LOCUS       DBMDSQ010000001        25203 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_695_length_25203_cov_2.386065, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000001 DBMDSQ010000000
VERSION     DBMDSQ010000001.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 25203)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 25203)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25203
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_695_length_25203_cov_2.386065"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(92..739)
                     /locus_tag="ACZPAX_00005"
     CDS             complement(92..739)
                     /locus_tag="ACZPAX_00005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1112310.1"
                     /translation="MLQTLERTNTFDARVLTASHAGALRELTGDTGLPLLPSLHPALE
                     EGQIVGDYAGQGELQAAAALLPLYADDPLPTSLRAAGYGADGQGAVLTPPVLRSDYTQ
                     LRHFLRLALRWATERYASYHVWAVLPLDLEHPEACDDLCAQYLSTGLTLRGMRPMAGA
                     DQMLIFSARGLAQWRDPLRRCHLADPALPRVLERGYAAADFGWGKDGLELVLRPV"
     gene            complement(994..1752)
                     /locus_tag="ACZPAX_00010"
     CDS             complement(994..1752)
                     /locus_tag="ACZPAX_00010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1112311.1"
                     /translation="MAWVTKDSTETYNQTPEPPREYTKTEKAANWWHYHWGIVVIVVL
                     CAFFGIWIIKDTVFQTRPDVQIAYVGTHDLPADTVTALQDALTPFCEDTNGDGKVVVQ
                     VDTYNVDFDADNVNTDAYSQMAGVTRLSAELSSGGKTYIFLLEDPAAFESSTGALQYL
                     DGTVPDDPDAANPDWTEMVYRWTDCPVLAGLELGDYDGYTLLDDATGTNQSVLATLYV
                     GRRGVWDEKQAATYEGCAELWDTLTAGAVSTAAE"
     gene            2043..2858
                     /locus_tag="ACZPAX_00015"
     CDS             2043..2858
                     /locus_tag="ACZPAX_00015"
                     /inference="COORDINATES: protein motif:HMM:NF016726.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HIR1112312.1"
                     /translation="MSNQPETNETPATGYTPAPRKVGLPRFFELLGRDLWAFYRASFL
                     CLLGFLPGTALAVFGQMGGSALLTVLGGLLGGLIGGPFLSGMIDTTLRALRDEPGYWW
                     HTYKRAWKQNWKQSLLPGALLGLFVGSWSWMLRAQAAAGNTSTMMWVASLAGIFVCTG
                     FFSWLLAQVPLVDLPLPQLAKNAGLMFFGFFPRTLAAALLLAVYWGLTLLYLPFTIIT
                     LLAFGFWLPVVIALMILYPGLDKVFKLEETLSARRDAEIEERIAESQPTFHNK"
     gene            2871..4184
                     /locus_tag="ACZPAX_00020"
     CDS             2871..4184
                     /locus_tag="ACZPAX_00020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1112313.1"
                     /translation="MERNTVLLRETEAFMRGELDNVTVSQNSIVLDLVQGSYVPYGCY
                     TSAPISMPVFDALRVSWNAASPRGTAVEAQARVLVDGNWTPWVGFGKWSPYLHRESPA
                     YQERGPVQRWPAHLQLDSKYATQAQLRIYLYSKDEKATPAVTLLGVSTHVVDVIPARG
                     RPVNARLHLMPYAAARRTPALRPWMDLANCLASLTNRWGADLLPEEFAQVLRDWRAPD
                     DCDPRNLSFAAAAAAQWGFPAWVAYGDLALLRAEARAGCGAVVGLQSSPEQIAAGAPE
                     RRFAAVRGFASIDGSPKVLLCDPWASENDFDCETDMPLDDFMVAWDNLALLMRQRKNN
                     TSPLGRTRCSVWLRPVGTDAPGVYHMYINGEEHLLADDFCARGGVLAYSLPDAQPHAT
                     TAHRQFTYVEPTQGGILLERGDTPRKYTVYAIDSAGGMLVGDVTV"
     gene            4195..4740
                     /gene="yfcE"
                     /locus_tag="ACZPAX_00025"
     CDS             4195..4740
                     /gene="yfcE"
                     /locus_tag="ACZPAX_00025"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015538185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HIR1112314.1"
                     /translation="MKLLIASDIHGAAGYCRQLLDAWDREGADRLLLLGDVLYHGPRN
                     DLPPDYAPKEVIALLNARKDKIFCVRGNCDTEVDQMVLEFPILADYAVFTAGRRLVYA
                     THGHVYNTAHLPPLQAGDILLHGHTHVPAWQAFGNDNYYLNPGSTSIPKENSAHGYMT
                     LKDGVFTWKTLDGEKYHEVKV"
     gene            4873..5706
                     /locus_tag="ACZPAX_00030"
     CDS             4873..5706
                     /locus_tag="ACZPAX_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007048366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MYG1 family protein"
                     /protein_id="HIR1112315.1"
                     /translation="MKIPAKGFTHGGKFHADDVFSTALLQILRPDIQVTRGFVVPDDF
                     DGIVYDVGGGMFDHHSEPRECRPNGVPYAAFGLLWRVVGAQLVGEHQARLMDENFIQP
                     LDLNDNTGEQNSLADAIGSFNPLWDSKDDPDECFWRAVPVAKQILENEIAAANAVNRA
                     DDTVRRAYANMRDGIVVLPAYMPWKNGLYKTDAIFVVYPSQRGGYSAQCVNDHRTKRS
                     KQPFPVAWAGKPEEQLRQISGLGLRFCHPSRFLITADDKATAIEACRRTLRAAGKKVS
                     E"
     gene            5703..6599
                     /locus_tag="ACZPAX_00035"
     CDS             5703..6599
                     /locus_tag="ACZPAX_00035"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR1112316.1"
                     /translation="MSAEGSEIYGPAPSTAADREPTPAPAWVYLLRCPDGSLYGGWTN
                     DLARRLKAHRSGKGGAKYTKSHGRDAVRLAYAEKCADKSAALKREAALKKLPKAEKEA
                     LAEKWAADNKITLRMATPDDAAAVCALYNWYVRHGVQTFQYTPSTVEEYRENIAHTLQ
                     NAPFLLAESADGRLQGFACAHLWHEREAYAWDVETTAYCAPDCIGQGVGGRLYRALIA
                     LLKKQGYYNAFALVTGSNRQSNNFHKALGFTKMGIEKRTGYKFGQWLDLDYWVLPLQD
                     GEGEPQPVRKQLTAEEIAEALK"
     gene            complement(6662..6982)
                     /gene="trxA"
                     /locus_tag="ACZPAX_00040"
     CDS             complement(6662..6982)
                     /gene="trxA"
                     /locus_tag="ACZPAX_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HIR1112317.1"
                     /translation="MSIKLTEQNFDTEVLQSAMPVVVDFWAEWCGPCKMMGPVLEEVA
                     AAHPTVKFGKLNTDENLKLAMARKIDAIPALLLFKDGREVSRSVGYRPAAELEAWLRE
                     NGAI"
     gene            complement(7038..9296)
                     /locus_tag="ACZPAX_00045"
     CDS             complement(7038..9296)
                     /locus_tag="ACZPAX_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009983302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HIR1112318.1"
                     /translation="MNVEELVSALTLEEKAGLCSGADFWHTKAVERLGIPAIKVSDGP
                     HGLRTQKEGSTDPNDAIEAVCFPAGCAAAASFDPALTRRMGAALGREARASGVQVLLG
                     PAVNIKRSPLCGRNFEYYSEDPYLAGELAAGFIQGVQSEGVGTSIKHFAANSQETRRL
                     SADSRVDERTLHEIYLPAFETAVKKARPWTVMCSYNRVNGDYASENKPLLTDILRDDW
                     GFGGFVMSDWGAVNDRVKGVAAGLELEMPGSGGVYDRKIIEAVQNGTLDIGVLDKAVA
                     RILNIVLRAADLAKLPAVFDHAADHKLAVELAKQSAVLLRNLGALPLHKGQKVAYIGA
                     FADHPRYQGGGSSHVNTTAISAMDAAIMNDRRVSYIEGFPADRDQRDEAEFLRAVTAA
                     EAADVAVIFAGLPDSFESEGADRRHMRLPDCQNNLIARVAAVQKNTVVVLHTGSPVEC
                     PWADDVSSVLCMYLAGEGVGEAADALLWGDANPCGRLPETWPLRLEDTPCYLDYPGDG
                     VTADYREGVYVGYRWYDARKMPVRWPFGHGLSYTGYVYRNAELSCDTMAADGEVTVRV
                     TVKNSGAMPGAEVVQLYVADATGTPVPGGRVPQALRGFAKVDLQPGEERVVTFTLTPR
                     DLSRYSAEIHDWYAAPGRYEVRIGHSSRDIRSTLPLHYAGAKLLPLTVDETTPLGILL
                     ADPRTAPIVRGMLDAQVQAMANGGGDGLMPPEAMIQMFDGLTLRNMINFGGPQAEERL
                     NAMLAKLKEAVQ"
     gene            9447..10109
                     /locus_tag="ACZPAX_00050"
     CDS             9447..10109
                     /locus_tag="ACZPAX_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007045703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR1112319.1"
                     /translation="MNNILIAEDEHLIARLIEMTLTRAGYTCTVAEDGRTAADLIEKN
                     NFDMALLDIMLPGLDGYALLESLRPQGVPVIFITAKSAVKDRVLGLRLGADDYITKPF
                     AAEELVARMESVLRRTGRGGRELRAYDVVLDTVDRVARQNGEPLDLRRREFDLLELLM
                     RNRGAALYRDVIFERVWGELPDTESLRTVDTHIARLRKKLGWTDKQIETVFKIGYRLR
                     KE"
     gene            10312..>11489
                     /locus_tag="ACZPAX_00055"
     CDS             10312..>11489
                     /locus_tag="ACZPAX_00055"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR1112320.1"
                     /translation="MRYYQKLVSLLVLVLAASFGVGGCVLLYSDFAVQRGRMATANAA
                     AHAQTCTLLQTEILDLQRRGTTMDDAALTARVEARDTPAALWRGDTLICATLPGLENF
                     SLENAATVTVRTEESVYAVYASDLQGGLRLVTAYDLTGLYRDRDAALTRFLLLEAAVL
                     AAAAAVTALLAARLTRPLAVLTDAGQQIAAGDYVRRTALHTGDEIETLSASFDKMADA
                     VQEKIADLEADVQKREDFVGAFTHELKTPMTSIIGYADILAAMQTDPDEQREAAAAIA
                     HEGRRLESLSHKLLALLGLAECPLSPAAVPLAALWPRLHAACPGVNLRTPAAPAPTVQ
                     GDADLLLDLLCNLVQNAAKASKPGAPVVVLCTADADTATLTVADRGCGIPPDQLARVT
                     "
     assembly_gap    11490..11499
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11500..11643
                     /locus_tag="ACZPAX_00060"
     CDS             <11500..11643
                     /locus_tag="ACZPAX_00060"
                     /inference="COORDINATES: protein motif:HMM:NF014567.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR1112321.1"
                     /translation="QGGSGLGLALCQRIAVAHDGTLKIESEPGKGTRVSVTLPLWKKE
                     ADA"
     gene            11640..12497
                     /locus_tag="ACZPAX_00065"
     CDS             11640..12497
                     /locus_tag="ACZPAX_00065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1112322.1"
                     /translation="MSLWRYPRTAAALCIAAVLACAAAPAAFLAASDAAYFSRVETVA
                     DPYRAPTPTADDYYILRQLAARGQDTTARQTTPQQEQDAAPQTPKVYIGASASIESMT
                     WADTAATTAAENALWQLAESSAVPRFWVEQTVGDTVDYGSYTDANGQYYNLNSAYCTT
                     DSLGFVTVRKFARENDTLFTRYSITMDGRTGMIVEVWFSLPAADAAETPLPGADALRS
                     FAAQAGLESLGDWAAPESSPYDCALYSANGGALITASTHPYTYTGYTAADGAERWYYS
                     LALQIAPQT"
     gene            12518..13954
                     /locus_tag="ACZPAX_00070"
     CDS             12518..13954
                     /locus_tag="ACZPAX_00070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1112323.1"
                     /translation="MKKLIPLILTAAMTLTLAACTAAPDPAPLATPTPEPTAAAAAPT
                     PEPMAAAAAPTPAPAADFYTLKSYNGSYDDGTAYYEFVQRLGCTLLLKTDYAAAVQSV
                     CCAVPGCGHDTDACPAYFPGRAGRYTVVAAGDTVYVWHTSFFYTDQSWDDFWAEKQAS
                     GIRNAAPYDTLTDDEFEADYRGIWAEQSTPPCLYAIDPAAGKTRTDLPLTYRDYTMDA
                     CDGSTLYGEQMIAPYSSQASAGKIGPTPACRIDLATGQAETFALEPTEHYLAGFDGAL
                     LTRRYVTDAPLPTDAEQYAAAIQSATVKIDRLDPRTGARTSLTERPYSDADYEKNGCF
                     IGVYNGKAYFEERTIIPGSGFRRTVLTAVDAEGRAETVWDPWPQAEWVLGDDGGRYIW
                     LYRDNYNTSYAACALLDTETGQITPVTQALQTGSGAVSLRGKAHDGRWLVVIGADSAG
                     RTTAYGLIDADQFAAGSTDWQPVAMWQG"
     gene            14131..15435
                     /locus_tag="ACZPAX_00075"
     CDS             14131..15435
                     /locus_tag="ACZPAX_00075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1112324.1"
                     /translation="MKKLIPLILAAALTLAACASASDETPLTTPTPAPEAPEPTSTPA
                     PATTDFYPLESYTTAFNTGDAYYELVPYPGHALLLKTDYATAAQTVCCTMPNCAHDSE
                     ACPAYFPGRARRYTVFTAGDTLYVYHTLFYRNTQTWEKYFDEYGKPQLEHPDQSFEGL
                     TADEITADFYGRWTEQTTPPCLYTVHPGGGKVRTDLPEEVRDYVMAYCDGAAVYGAAW
                     NDENGTTATGCRISLADGAVQTFPLLTQEQFLDVCGDALVTSHIVTDAPLPTDREQYQ
                     AAIQSAETEYDLYDPNDGTRTRLAAYPYDTSALLGVYGGKAYFEKRQPIPYAAYEPVA
                     VETLDIATGTTETIWTPEQGAQELPLGDAEGHSLRMSEGNVIVGQHTSLPVDFRAAAA
                     DGRWLVMTGKDETTNRENYALVEPEQFMAGSAEQETVTMWQG"
     gene            15551..16816
                     /locus_tag="ACZPAX_00080"
     CDS             15551..16816
                     /locus_tag="ACZPAX_00080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1112325.1"
                     /translation="MKKLIPLILTAALTLAACASAPDTASTPLANSTPEPTAATTPAP
                     TTAPFYPVANSYNNGDTYYAFVHRGEDSLLLKTDYAAATQTVNCAVPGCAHDSDACPA
                     YFTDDPGRNLVITDDPLRVCHVEDRGCLVQIYTVDPAEGKAMQEINGVGNCDIAYCDG
                     TALYGIDKTVLPSEATPACRIDLASGQLDRFTMLPSELMLGCYDDGLLTVHYVTDAPL
                     PKNGEEYAAAVQSATLEFDCWDPRTGTRTKLAERLYNPTDARFSGYDGTANGKLYFVD
                     YLPQSDGTDIKLSLTVVDPAMGTEEKMWDTWPDEHFGLSIGQRILPVHGDQSTRWLGM
                     CYSNSGTNREIPYFMDLETGKIKEVTQKRESLSYDRRVTVLAQTNDGRWLIETAADEQ
                     RSCLGMALITPEQLLAGSTDWQEITMWQG"
     gene            17006..18352
                     /gene="gdhA"
                     /locus_tag="ACZPAX_00085"
     CDS             17006..18352
                     /gene="gdhA"
                     /locus_tag="ACZPAX_00085"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007045710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HIR1112326.1"
                     /translation="MAIQNAYLQGVYEGLAKRNPEQKEFLQAVEEVLESLEPVVAAHP
                     EYEKAGLIERLVEPERIIMFRVPWVDDAGKVQVNRGYRVQFNSAIGPYKGGLRFHPSV
                     NLSILKFLGFEQCFKNSLTGLPMGGGKGGSDFDPHGKSDAEVMRFCQSFMTELYRHIG
                     PDTDVPAGDIGVGGREVGYLFGQYKRLQNEYTGVLTGKGIPFGGSLARTEATGYGLCY
                     FTKEMLADAGKSFEGQRVVISGSGNVATYANQKATQLGGKVIAMSDSNGYIVDENGID
                     YKVIKEIKEVKRARIKTYLEYVPTAKYVEGSQGIWTVPCDIALPCATQNELRLDGAKA
                     LVANGCYCVAEGANMPSTPEAIAYLQSHGILFAPAKASNAGGVATSGLEMSQNSLRLS
                     WTFEEVDERLHNIMVNIYKTAATTAEKYGMKGNLVAGANIAGFEKIASAMMSQGIV"
     gene            18471..18546
                     /locus_tag="ACZPAX_00090"
     tRNA            18471..18546
                     /locus_tag="ACZPAX_00090"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18504..18506,aa:Met,seq:cat)
     gene            complement(18753..18905)
                     /locus_tag="ACZPAX_00095"
     CDS             complement(18753..18905)
                     /locus_tag="ACZPAX_00095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1112327.1"
                     /translation="MQTPTLDAALLEVKQQIEKALRQYKNPPIPTKQEQIVVPNNSVL
                     VLVKAS"
     gene            19233..24371
                     /locus_tag="ACZPAX_00100"
     CDS             19233..24371
                     /locus_tag="ACZPAX_00100"
                     /inference="COORDINATES: protein motif:HMM:NF014149.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HIR1112328.1"
                     /translation="MRKAKRRCQQLVAAAMCVGMLAGMVPTQVFAEEAQPAAAIAQQE
                     QAPAPSANETETPAPTEEPAAEQTPAPTEEPAAEQTPAPTEEPAAEQTPAPTEEPAAK
                     QTPAPAEEPAAEQTPAPAEEPAAEETAAQAAEVMSAAVQDSENENFGKVLEDQDLVAV
                     RSGASLVESLSTGKQVKVTAEWISGSNSAVDGGGALQEGDAFFKRSRFTLYANVKFDA
                     AHDNTSALLVGSDAGANFRIVPCKTDGTAVLKVNNGTEHKLSKAMTAGEWNALALVYA
                     ENDTEGTVAVYLNGEEVLPATGIGFKLSEKSGIVGAVGATFGTGFMRTGLYDELVVTG
                     AADAEAAKVETAARKAAADAVVAVDGVVTVTGAEVEQAAQNVNGWTYKGLGMLNGNST
                     SNLLLDYKAESPDAYWAMMRYLFGGDYPLFSNIKMEMGNDGNNSTGAEACTMRYEDEE
                     ADASRSPGFVMAADAKTINPNVKVSILRWGCPNWVNAYKGTDWYAANYKWYKETIFDA
                     YEKYGYVVDYINPDTNETGNPDSGIIKYFANAIANETDFPEYFTEEAKQAYRSIKIVA
                     SDENKGLKIVPMMRGDKDLYDAVDAIGFHYRTNATDDYIKMADVDDKEVWYSEGCATF
                     GYSELQENKTAEYGAGTIGGYQSPLALLDSFPNAFVGSRRTMYMFQPAIGAFYEGIQY
                     GHKELLSARDPWSGYIHYDPVLYMLGHITKFAKTGWENASNTAGIWRVLPGATFASFG
                     ASSNEHATAGIDGNASYMTLAAPDKQNFSTVFVNNTRNEKTFAVKLSDMALKAEALHI
                     WTTATDSYMQKGEDAKIEGGIAYVTVPAYSVVTATTLDTEPERFPTEDGSGDYIQTAT
                     RAVLDTDATGKKADTADDYLYADNFDYKEEELNYIVDRGNEPRYMLDTHGAWIVENGR
                     LKQENAAGVTQWNGGDPATIVGDWRWMDYSASVDMELPNADANRYERLTIRAQTGMNW
                     NNSGYTLELNGAGSWKLFRIGSAVASGSVAKDDAGKYNVKLIGLGDTVYAYINGTLVT
                     SYTDANPMLSGRVKISSNWTQVYADNLEIRTVKGGIPYATAMIDGQDDGVVYEGAWAI
                     NNPGGGSADNWYRTMSEASTAGAAFTFAVDGSGFAIMGSNNGSAVIDVYVDGVMKVEN
                     AATKAAHTRGEAYILSDLAAGKHTVKVVLKSGTLKVDALNTIGNRLPAAEGAVTEVLT
                     KLPALESYVTGSGIGSLPAAVEVKKTDGTTATLPVDWNVDMNALNANAYGAAEIQGTV
                     KGAVNPLGEKLTVSVKIGEVIPGDTVVFIDSVAGDPAAVSGGTTESYTLYKKALGSKL
                     LNEAFDQRKTADNDWGLVDTDAGTKGYSSTADKTATGIYGRENKAGETLTYALTLPAG
                     SYILTSAHHEWWGQNRPMTAVVTDAEGNALSETATLNLNGSSGDILNKAVFKLDGQKT
                     VYYTVTATGSQAPVISWLGVQRQPAETKVVVTKGLTSVPEALQNTAFTTTEKIEKELV
                     KQLSDEISGCKVSIYDVKLMLDFGDGSLIEAEENSFPAGGMDVWFDLPDGVTAENFSQ
                     FDIEVAHMLCTRDHAGEVELLKDPELDAANGKLKVHVGSFSPFAIGWKAKPASGPVVT
                     PGTGSTGTTPGGSTGSNTGSNTNAGNSSNTAAGNAGGTAAAASGTAKPAGAADNKAAV
                     PAKSGLIPQTSDSFPLLPVAGIALVSLAAVGLLALLKKKKHETR"
     gene            complement(24498..>25203)
                     /locus_tag="ACZPAX_00105"
     CDS             complement(24498..>25203)
                     /locus_tag="ACZPAX_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005943364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carbohydrate-binding protein"
                     /protein_id="HIR1112329.1"
                     /translation="DDPIKAIQKVEEIGTPGQYIVIQFEDTMAPALVYVVKREINFHI
                     PFGEQAITYSPKSFSGCCHIIRARFATEAEIKARRNLAFNPYDEHGDTGFFPHASANV
                     ETRGEAVFAARNAVDGVYENDAHGTWPYQSWGINRDPNAALTLDFGRAVIVDELRLTL
                     RADFPHDSYWTQATVAFDDGSREVLQLTKTAAPQTFAITPRTAKSLKLFELKKAEDPS
                     PFPALTQLEAWGTEAP"
BASE COUNT         5057 a         8075 c         7668 g         4393 t
ORIGIN      
        1 cccctccgcg gcgggcagag gactgcccgc cctacaaggt gaaggtccgt agggcggcca
       61 gccctctggc cgccgccccc acaatattgc atcacaccgg cctcagcacc aactccaatc
      121 cgtctttccc ccagccgaaa tcggcggccg catacccgcg ctccaacacg cggggcagcg
      181 ccgggtcggc cagatggcag cggcgcagcg ggtcacgcca ttgggctaac ccccgcgctg
      241 aaaaaatcaa catctggtcg gccccggcca tcgggcgcat cccgcgcagg gtcagcccag
      301 tggagagata ctgcgcacac aggtcatcac acgcctccgg gtgttccaag tccagcggca
      361 gcacggccca gacgtggtag ctggcatagc gctcggtggc ccagcgcagc gccaaccgca
      421 gaaaatgccg cagctgcgtg taatcgctgc gcagcaccgg cggcgtcagc accgcgccct
      481 ggccgtcggc cccataaccg gcggcccgca ggctggtggg aagcgggtcg tccgcataga
      541 gtggcagcag cgcggcggcg gcctgcaatt ccccctgccc ggcgtaatct cccacgatct
      601 gaccttcctc cagcgccgga tgcaggctgg gcagcagcgg cagcccggta tccccggtca
      661 gttcccgcag agccccggcg tggctggcgg tcagtacgcg ggcgtcaaaa gtgttggtac
      721 gttccaaagt ttgcagcata agtaaaattc cctccgtaaa aagtcattac agaccttatt
      781 gtaaggtacg gcgggcataa aaaatgtcgc atcccggggt gggatgcggc attttttagc
      841 gatgctgagc tttgcgcttt tcgttgatca aatcctcaaa actgcgctgc tttgtaaaaa
      901 aacgcttgta cgggttttcc ggcagcgccg gggtgctggg cttgggctgc acctttttcc
      961 gcagatgccc cttcaggggc ggcagcgccc ggctcattcg gcggcggtgg aaacagcccc
     1021 tgcggtcagg gtgtcccaca gctcggcgca gccctcgtag gtggctgcct gcttttcatc
     1081 ccagacgccg cgccgcccca catacagggt ggccagcacg ctctggttcg tgccggtggc
     1141 gtcgtccaac agggtgtagc cgtcgtagtc gcctagttcc agcccggcca gcaccggaca
     1201 gtcggtccag cggtagacca tctcggtcca atcggggttg gcagcgtcgg ggtcatcggg
     1261 cacggtgccg tccaggtatt gcagcgcacc ggtgctgctc tcaaaggcgg cgggatcctc
     1321 cagcaggaag atataggtct tgccgccgct ggaaagctcg gcgctcagcc gggtgacacc
     1381 ggccatctgg ctgtaggcgt cagtgttcac attgtcggcg tcaaaatcca cgttgtaggt
     1441 gtccacctgc accacgacct tgccgtcgcc gttggtatcc tcgcagaagg gcgtcagcgc
     1501 gtcctgcaaa gccgtcacgg tgtcggcggg caggtcatgg gtgcccacat aagcgatctg
     1561 cacgtcgggc cgggtctgaa agacggtatc cttgatgatc cagatgccaa agaacgcgca
     1621 gagcaccacg attaccacga tgccccaatg gtagtgccac cagttggcgg ccttttcggt
     1681 tttggtgtac tcgcgcgggg gttccggcgt ctggttgtaa gtttcggtgc tgtctttcgt
     1741 gacccaagcc atagtgtgga tacactccct ttataatatc tttcctatta taccacaaat
     1801 cgaccgggaa aattgtaaac ttattttgac cacgccttgc aagaggagta aaaacagggt
     1861 atgataaact tataaatgta tagaggaaaa gcctcccttg tcaaagggag gtggacgccg
     1921 aaggcggccg gagggattcc gtgacggttg cctttatgcc cgcaggaatc ccccagtccg
     1981 cttacgcgga cagccccctt tgacaagggg gcctttacgc tacgaaaaaa gggagaacca
     2041 ccatgtccaa ccaacctgaa accaacgaaa cgccggccac cggctataca cctgcgccgc
     2101 gcaaggtcgg actgccgcgc ttttttgaac tgctcggccg cgacctgtgg gcgttctacc
     2161 gcgccagctt tctgtgcctg ctgggctttc tgcccggtac ggcgctggcg gttttcggcc
     2221 agatgggcgg ctcagcgctg ctgaccgtgc tgggcggcct gctgggcggc ctgatcggcg
     2281 ggccgttctt gtccggtatg atcgatacca cgctgcgcgc cttgcgcgac gagccgggct
     2341 actggtggca cacctacaag cgcgcctgga aacaaaactg gaagcagagt ctgctgcccg
     2401 gtgcgctgct gggcctgttt gtcggcagtt ggagctggat gctccgcgcc caggccgcgg
     2461 cgggcaacac cagcacgatg atgtgggtcg cctcgctggc gggcatcttt gtctgcacgg
     2521 gctttttcag ctggctgctg gcccaggtgc cgctggtgga tctgccgctg ccccagctag
     2581 ccaaaaatgc cgggctgatg ttcttcggct tcttcccacg cacgctggcg gcggcgctgc
     2641 tgctggccgt ctactggggg ctgacgctgc tctacctgcc gtttaccatc atcacattgc
     2701 tggccttcgg cttctggctg ccggtggtca ttgcgctgat gatcctgtac cctggcctcg
     2761 acaaggtatt caagctggaa gagaccctca gcgcccgccg cgacgccgaa atcgaggagc
     2821 gcatcgccga gagccagccg acgttccaca acaaataagg gggccgggct atggagagaa
     2881 acaccgttct gctgcgcgag accgaggcgt tcatgcgcgg cgaactggac aacgtgaccg
     2941 tctcacagaa cagtatcgta ctggatctgg tgcagggcag ctatgttccc tacggctgct
     3001 acaccagtgc gccgatttcg atgccggtgt tcgatgcgct gcgggtcagc tggaacgcgg
     3061 cctcgcccag gggcacggct gtggaggccc aggcccgcgt actggtggac ggcaactgga
     3121 cgccctgggt gggctttggc aaatggagcc cctacctgca ccgcgaaagc ccggcctatc
     3181 aggagcgcgg gccggtgcag cgctggcccg cccacctgca gctggacagc aagtatgcca
     3241 cccaggccca gctgcgcatc tacctgtatt ccaaggatga aaaggccacc cccgctgtta
     3301 cgctgctggg ggtcagcacc catgtggtgg atgtcatccc cgcccggggc cgcccggtca
     3361 acgcccggct gcacctgatg ccctatgctg ccgcccgccg cactcccgcg ctgcggccct
     3421 ggatggatct ggcaaactgc cttgccagcc tgacgaaccg ctggggcgcc gacctgctgc
     3481 ccgaggaatt tgcccaggtg ctgcgggact ggcgcgcccc cgacgactgc gacccccgca
     3541 acctgagctt tgccgctgcg gctgccgccc agtggggctt ccccgcctgg gtggcctacg
     3601 gcgatctggc cctgctgcgt gccgaggccc gggccgggtg cggcgcggtg gtggggctgc
     3661 aatcctcgcc ggaacagatc gccgccggtg cacccgagcg ccgctttgcc gccgtgcgcg
     3721 gctttgcgtc catcgatggc agcccgaaag tgctgctctg cgacccctgg gccagcgaaa
     3781 acgattttga ctgcgagacc gatatgccgc tggatgattt catggtcgcg tgggacaacc
     3841 tggccctgct gatgcgccag cgcaaaaaca atacctcgcc gctgggccgc acccggtgca
     3901 gcgtctggct gcgcccggtg ggcaccgatg cgcccggcgt ctaccatatg tacatcaacg
     3961 gcgaggagca cctgctggcc gatgattttt gcgccagggg cggcgtgttg gcatacagcc
     4021 tgcccgacgc ccagccccat gccaccaccg cccaccggca gttcacctat gtggagccga
     4081 cccagggcgg tatcctgctg gaacgcggcg acaccccgcg caagtacacg gtctacgcca
     4141 tcgactccgc cggcggtatg cttgttggcg acgtgacggt gtaaggagga cgctatgaaa
     4201 ctgttgattg catcggatat ccacggcgcg gcgggctatt gccgccagct gttggacgct
     4261 tgggaccgcg agggggccga ccgcctgctg ctgttgggcg atgtgctcta ccacggcccg
     4321 cgcaacgacc tgccgcccga ctacgcgccg aaagaggtca ttgccctgct gaacgcgcgg
     4381 aaggacaaaa ttttctgtgt gcgcggcaac tgcgacaccg aagtggacca gatggtgctg
     4441 gaatttccaa tcctggccga ttacgcggtg ttcacggcgg gccggcggct ggtttacgcc
     4501 acccacggcc acgtgtacaa caccgcgcac ctgccgccgc tgcaagcggg ggatatcctg
     4561 ctgcacggtc atacccatgt gcccgcatgg caggcgttcg gcaatgataa ctattacctc
     4621 aaccccggct ccacctccat ccccaaggag aacagcgccc acgggtatat gacgctgaaa
     4681 gacggcgtgt ttacgtggaa aacgctggac ggggagaagt atcacgaagt aaaagtgtga
     4741 ccgcaggggc cgggtatgcc cggcccctgc aaagaacaag gcgtagggag cggcgtcctc
     4801 gacgccccgc agagggggca ccccccctgc gaccccaccg cacatccatt tatagataga
     4861 ggagtaccac ccatgaaaat ccctgccaaa ggttttacgc atggcggaaa attccatgcg
     4921 gacgatgttt tttccaccgc gttgctgcaa attctccgcc cggatatcca ggtcacccgc
     4981 ggctttgtcg tgcccgatga cttcgacggc atcgtctacg acgtcggcgg cggtatgttc
     5041 gaccaccact ccgaaccgcg ggagtgccgt cccaacggcg tgccctacgc ggccttcggc
     5101 ctactgtggc gggtggtggg cgcccagctc gtgggcgagc accaggcccg cctgatggac
     5161 gaaaacttca tccagccgct tgacttaaac gacaacaccg gcgagcaaaa ctccctggct
     5221 gacgcgatcg gcagcttcaa cccgctgtgg gactcgaagg acgaccccga cgagtgcttc
     5281 tggcgcgctg tgccggtggc aaagcagatc ctcgaaaatg agatcgccgc cgccaacgcc
     5341 gtgaaccgcg ccgacgatac cgtccgcaga gcctacgcca atatgcgcga cggtatcgtc
     5401 gtactgcctg cctatatgcc atggaaaaac ggtctgtaca agaccgatgc aatttttgtt
     5461 gtctacccca gccagcgcgg cgggtacagc gcccagtgcg tcaacgacca ccgcaccaag
     5521 cgcagcaagc agccgttccc ggtggcctgg gccggtaagc cggaggaaca gctgcgccag
     5581 atcagcggcc tgggcctgag gttctgccac cccagccgtt tcctgattac cgccgacgac
     5641 aaggcaacgg ccattgaggc ctgccgccgc acactgcggg ccgccggaaa gaaggtaagc
     5701 gaatgagcgc agagggatcc gaaatctacg gaccggcccc cagcaccgcc gccgaccgcg
     5761 aaccgacccc ggcacccgcc tgggtctacc tgctgcgctg cccggacggc agcctgtacg
     5821 gcggctggac caatgatttg gcccgccgcc tgaaagccca ccgcagcggc aagggcgggg
     5881 ccaagtacac caaaagccat ggccgcgacg ccgtgcgcct ggcctacgcc gaaaaatgcg
     5941 ccgacaaaag tgcggctctg aagcgtgaag cagcgctgaa aaaactgccg aaggccgaga
     6001 aagaggccct ggccgaaaag tgggccgccg acaacaaaat taccctgcgc atggcgacgc
     6061 ccgacgatgc cgccgccgtc tgcgcactgt acaactggta cgtgcgccac ggcgtgcaga
     6121 ccttccagta cacgccctcc acggtggagg aataccgcga aaacattgcg cacacgctgc
     6181 aaaacgcgcc gtttctcctg gccgaaagcg ccgatggccg gctgcagggc ttcgcctgcg
     6241 cacacctctg gcacgagcgc gaggcctacg cctgggacgt tgagacgacg gcctactgtg
     6301 cgccggactg catcggccag ggcgtgggcg ggcggctcta ccgcgcgttg atcgccctgc
     6361 tgaaaaagca ggggtattat aacgcctttg cgctcgtaac cggcagcaac cgccagagca
     6421 acaacttcca caaggcgctg ggcttcacca aaatgggtat cgaaaagcgc accgggtaca
     6481 agttcggcca atggctggat ttggattatt gggtgctgcc gttacaggac ggtgagggcg
     6541 aaccccagcc ggtgagaaag cagctgaccg cagaagaaat tgcggaagct ttaaaataag
     6601 atgaaacaaa ccgcacctgc cctttttgac gaaggcaggt gcggctcttt ttatacaggt
     6661 gtcaaatcgc tccgttttcc cgcagccagg cttccagctc ggcagcgggg cggtagccga
     6721 cggaacggct gacctcccgg ccatccttaa acagcagcag cgccgggatg gcgtcgatct
     6781 tgcgggccat ggccagcttc aggttctcgt cggtgttcag cttaccgaat ttcacggtgg
     6841 ggtgcgcggc ggccacttcc tccagcacag ggcccatcat cttgcagggg ccgcaccact
     6901 cggcccagaa atccaccacg accggcatgg cgctctgcaa aacttcggtg tcaaaattct
     6961 gttcggtgag tttgatagac atagatatac cctttctttt tatggggcgg gccgtcaggg
     7021 acgccgcccc cctgcaatta ttgtacggct tcttttaatt ttgcaagcat ggcattcagc
     7081 cgttcctcgg cctgcggccc gccgaaattg atcatgttcc gcagggtcag gccgtcaaac
     7141 atctggatca tcgcctcggg cggcatcagg ccgtcgccgc cgccgttggc catggcctgg
     7201 acctgggcgt ccagcatccc gcgcacgatg ggcgcggtgc gcgggtccgc cagcagaatg
     7261 cccagcggcg tcgtctcatc cactgtcagt ggcagcagct tcgcccccgc ataatgcagc
     7321 ggcagcgtgg atcggatatc ccggctggaa tgtccgatgc gcacctcata tcgcccgggc
     7381 gcggcgtacc aatcgtgaat ttctgcgctg tatcggctta gatctcgtgg tgtcagggta
     7441 aacgtcacaa cgcgttcttc cccgggctgc aagtccacct tcgcaaaacc gcgcagggcc
     7501 tggggcacgc ggccgcccgg caccggcgtg ccggtggcgt cggccacata caactgcacg
     7561 acctctgcgc ccggcattgc gccgctgttt ttcacggtca cgcgcacggt cacttcgccg
     7621 tcggcggcca tcgtgtcaca gctcagctct gcattccggt agacgtatcc ggtgtagctc
     7681 agcccatggc cgaagggcca gcgcaccggc attttgcggg cgtcgtacca gcggtagcct
     7741 acatagacgc cctcgcggta gtcggcggtc acgccgtcgc cgggatagtc caggtagcag
     7801 ggcgtgtcct ccagccgcag gggccaggtc tccggcaggc ggccgcaggg gttggcgtcg
     7861 ccccacagca gggcatcggc ggcctcgccc acgccctcgc ccgccaggta catacacaaa
     7921 acagacgata catcgtccgc ccaggggcat tccaccgggg agcctgtgtg cagcacgacc
     7981 acggtgtttt tctgcacggc ggccacgcgg gcaatcaggt tgttctggca gtcgggcaga
     8041 cgcatatggc ggcggtccgc gccctcactc tcaaagctgt cgggcaggcc cgcaaaaatc
     8101 acggccacat cggcggcttc ggcggcggtc acggcccgca aaaactcagc ctcgtcccgc
     8161 tggtcgcggt ctgcggggaa accctcgata tagctgacgc ggcggtcgtt catgatggcg
     8221 gcgtccatcg cgctgatggc cgtcgtgttc acatggctgg atccgccgcc ctgatagcgc
     8281 ggatggtcgg caaacgcgcc gatgtaggcc accttctgcc ccttgtgcag cggcaacgca
     8341 cccaggttgc gcagcagcac ggcgctctgc ttcgccagct ccacggccag cttgtggtcc
     8401 gccgcgtggt caaacacggc gggcagcttt gccagatcag ccgcgcgcag cacaatgttc
     8461 aaaatgcggg ccacggcttt gtctaaaacg ccgatatcca gcgtgccgtt ctgcacagcc
     8521 tcgatgattt tgcggtcgta cacgccgccg gacccgggca tttccagctc caatccggct
     8581 gccacgccct tgacgcggtc gttcaccgcg ccccagtcgg acatgacaaa gccgccaaag
     8641 ccccaatcgt cccgcagaat atcggtcagc agcggcttgt tctcgctggc gtagtcgccg
     8701 ttgacgcggt tgtagctgca catcacggtc caaggccggg ccttttttac cgccgtctca
     8761 aaagcgggca ggtagatttc gtgcagcgtg cgctcgtcca cacggctgtc ggcggacagg
     8821 cggcgcgtct cctggctgtt ggcggcaaaa tgcttgatgg aggtgccgac cccctcgctc
     8881 tgcacgccct ggatgaaccc ggctgcaagc tcgcccgcca ggtagggatc ctcggagtaa
     8941 tactcaaaat tgcggccgca gagagggctg cgcttgatgt tcaccgcagg ccccagcaga
     9001 acctgcacgc cgctggcccg ggcctcccgc ccgagggctg cgcccatgcg ccgggtcagt
     9061 gccggatcga aggatgctgc cgccgcgcac cctgcgggga agcacacggc ctcaatggca
     9121 tcgttggggt cggtgctgcc ttctttttgg gtgcgcaggc cgtgggggcc atcgctgacc
     9181 ttgatagccg ggatgcccag ccgctccact gccttggtgt gccagaaatc cgcgccggag
     9241 cacagcccgg ccttttcttc cagcgtcagg gcgctgacaa gctcttcgac gttcatggtg
     9301 gttctccttt tatagtatgg gtggtttccc acattataac acgccgaatg tgcaaaaata
     9361 aataaaaaag tgtaaaatta tagtgcccgt gtaacaagta ggaaacaact ggggtttata
     9421 atgcacccaa acgagggaga tttgccatga acaacatact gattgccgag gatgagcacc
     9481 tgatcgcgcg gctcatcgag atgacactga cccgcgccgg gtacacctgc accgtggccg
     9541 aggatggccg cacggcggcg gatctgatcg agaaaaacaa cttcgatatg gcgctgctgg
     9601 atattatgct gcccgggctg gacggttacg ccctgctgga aagcctgcgg ccccagggcg
     9661 tgccggtcat cttcatcacg gcgaaaagcg cggtgaagga ccgggtgctg ggcctgcggc
     9721 tgggcgcgga tgattatatc accaagccct tcgcggcgga agagctcgtc gcccggatgg
     9781 aatcggtgct gcgccgcacc ggccgcggcg ggcgggagct gcgggcctat gatgtcgtgc
     9841 tggatacggt ggaccgggtg gccaggcaga acggcgagcc gctggacctg cggcggcggg
     9901 agtttgacct gctggaactg ctgatgcgca accggggcgc ggcgctctat cgtgatgtga
     9961 tttttgagcg cgtctggggc gagctgcccg acaccgagag cctgcgcacc gtggacaccc
    10021 acatcgcccg cctgcgcaaa aagctcggct ggacagataa acagattgag acggtgttta
    10081 agatcggcta ccgactgcgg aaggaatgac aaaggccccc ttgtcaaagc gggctggggg
    10141 atatctgtgg ccatgcggaa gagtgcgcgt acccgtgaag ctgcaagaat ccctccgacc
    10201 tcgctaacgc tcggccaccg ctccccttct gacgttttgc gccacctccc ccgagccggg
    10261 ggagtctgtc tttgacaagg gaggctttta cgaccacaaa aggagtaccc tatgcgctac
    10321 taccaaaaac ttgtctcgct gctcgtcctc gtccttgcgg cgtccttcgg cgtcggcggg
    10381 tgcgtgctgc tgtacagcga ttttgccgtg cagcgcggcc gcatggccac cgccaacgca
    10441 gcggcccacg cccagacctg taccttgctg caaacggaga tcctcgactt gcagcgccgc
    10501 ggcaccacca tggatgacgc tgccctgacc gcccgcgtcg aggcccggga cacccccgcc
    10561 gccctctggc gcggggatac cctcatctgt gccactctgc cggggctgga aaacttttcc
    10621 ctggaaaacg ccgccaccgt cacggtccgc accgaggaaa gtgtctatgc cgtctacgcc
    10681 agcgatttgc aaggcgggct gcggctggtc acggcctacg acctgaccgg cctgtaccgc
    10741 gaccgcgacg ccgccttaac gcgctttttg ctgctggaag ccgctgtgct ggccgcagcc
    10801 gccgccgtca ccgccctgct ggccgcccgc ctgacccgcc cgctggccgt gctgactgat
    10861 gccgggcagc agatcgccgc cggggactac gtccgccgca ccgccctgca taccggcgac
    10921 gagatcgaga cattgagcgc gagctttgac aagatggccg acgccgtgca ggagaaaatt
    10981 gccgacctgg aagccgacgt gcagaagcgg gaggactttg tcggggcgtt tacccacgag
    11041 ctgaaaaccc cgatgaccag catcatcggc tacgcggata tccttgccgc gatgcagacc
    11101 gaccccgatg aacagcgcga agctgcggcg gctatcgccc acgaggggcg gcggctggaa
    11161 tccctgagcc ataagctgct ggcgctgctg ggcctggccg aatgcccgct gtcccccgct
    11221 gccgtgccgc tggccgccct gtggccgcgg ctgcacgccg cctgcccggg cgtgaacctg
    11281 cgcacccccg ccgcgcccgc gcccaccgtg cagggcgacg cggatctgct gttggatctg
    11341 ctgtgcaacc tggtccagaa cgccgccaaa gccagcaaac ccggtgcacc ggtggtcgtg
    11401 ctctgcacag ccgacgccga caccgctacc ctgaccgtgg ccgaccgggg ctgcggcatc
    11461 ccgccggacc agctggcccg cgtgaccgan nnnnnnnnnc agggcggcag cggcctgggg
    11521 ctggcgctct gccagcgcat tgccgtcgcc cacgacggca cgctgaaaat cgagagtgaa
    11581 ccgggcaagg gcacgcgggt cagcgtgacc ctgcccctgt ggaaaaagga ggcagacgca
    11641 tgagcctgtg gagatacccg cgcaccgccg ccgcgctgtg catagccgcc gtgctggcct
    11701 gcgccgccgc gcccgccgca tttttggccg ccagtgacgc cgcctacttt agccgcgtgg
    11761 agacggtagc cgacccttac cgggccccca cccccacggc ggatgactat tacatcctgc
    11821 gccagctggc cgcccgcggg caggacacca ccgcccgcca gaccaccccg cagcaggaac
    11881 aggatgccgc cccgcagacg ccgaaggtgt acatcggcgc atcagccagc atcgagtcca
    11941 tgacctgggc ggacactgcc gccaccactg ccgccgagaa cgcactttgg cagctggcgg
    12001 aaagcagcgc agtgccccgg ttctgggtgg aacagaccgt gggcgacacc gtcgattacg
    12061 gcagctacac cgatgccaac ggccaatatt acaacctgaa cagcgcctac tgcaccaccg
    12121 acagcctggg ctttgtgacg gtgcgcaaat ttgcccggga aaacgatacg ctgttcaccc
    12181 ggtacagcat cacgatggat ggccgcaccg gcatgatcgt cgaggtgtgg ttctctctgc
    12241 ccgccgccga tgccgccgaa acacctctgc ccggcgcgga tgccctgcgc agctttgcgg
    12301 cccaggccgg gctggaaagc ctgggtgact gggccgcccc cgaaagcagc ccctacgact
    12361 gcgccctgta cagcgccaac ggcggcgcac tcatcacggc ctcgacccac ccatacacct
    12421 acaccggcta taccgccgcc gatggggcgg agcgctggta ttacagtttg gccttgcaga
    12481 tcgcaccgca gacgtagatt aaaaaaggag gtcccccatg aaaaaactca tccccctcat
    12541 cctcaccgct gcaatgaccc tgactctcgc ggcctgcacc gctgcccccg acccggcacc
    12601 cctcgccacc cccacgccgg agccgacggc agcggccgca gcccccacgc cggagccgat
    12661 ggcagcggcc gcagccccca cgcccgcccc tgccgccgat ttctacaccc tgaaaagcta
    12721 caacggctcc tatgatgacg gcaccgccta ctacgagttc gtgcagcggc tcggctgcac
    12781 gctgctgctc aaaaccgact acgccgcggc ggttcagtcc gtctgctgcg ccgtccccgg
    12841 ctgcggccac gataccgacg cctgcccggc ttatttcccg gggcgggccg gccgttatac
    12901 ggtggtcgcg gcgggggaca ccgtgtatgt ctggcacacc tcgtttttct acaccgacca
    12961 aagctgggac gacttttggg cagaaaaaca ggccagcggc atccgaaatg cagcgcccta
    13021 tgacaccctg accgacgatg aatttgaagc cgactaccgc ggcatctggg ccgagcagag
    13081 cacgccgccc tgcctttacg ccattgaccc cgccgcaggc aagacccgca ccgacttgcc
    13141 gctgacctac cgtgactaca cgatggatgc ctgcgatggc agcacgctct acggcgagca
    13201 gatgatcgcc ccgtacagct cgcaggcgtc tgccggaaaa atcgggccaa cgcccgcctg
    13261 ccgcatcgac cttgccaccg gacaggcgga gacctttgcg ctggagccca ccgagcatta
    13321 ccttgcgggc tttgacggcg cactgctgac ccgccgttat gtgaccgatg cgccgctgcc
    13381 caccgatgcg gagcagtatg ccgccgccat ccagagcgcc acggtgaaga tcgaccgcct
    13441 tgacccccgc acgggcgcgc gcaccagcct gaccgagcgc ccctacagtg atgccgacta
    13501 tgagaagaac ggctgcttta tcggtgtgta caacggcaag gcctactttg aggagcgcac
    13561 gatcataccg ggaagcggct tccgccgcac ggtcctgacg gcggtggacg ccgagggccg
    13621 cgccgaaacc gtctgggacc cgtggccgca ggcggaatgg gtgctgggcg atgacggcgg
    13681 ccgttatatc tggctgtaca gagataacta caatacaagc tacgccgcct gcgccctgct
    13741 ggatactgaa acagggcaga tcacaccggt gacgcaggcg ctgcagaccg gcagcggtgc
    13801 cgtcagcctg cgcggcaaag cgcacgatgg ccgctggctg gtcgtcatcg gcgcagacag
    13861 cgccggcagg accacggcct acggcctgat cgacgccgat cagttcgccg ccggcagcac
    13921 ggactggcag ccggtggcaa tgtggcaggg gtaacgtcgc aaagctcctc cggcgtcgct
    13981 ccgctcgcca cctcccctgt gaggggaggc tccccgcggg caaagcccgc gcaaaaaggc
    14041 tccccttcca ggggaactgg tcgagtttac gaggcctgag gggctttgca gccttgccat
    14101 cacagctcac atcaaaaaaa ggaggttccc atgaaaaaac tcatccccct catcctcgcc
    14161 gccgccctca ctcttgccgc ctgtgcatct gcctcggatg aaactccgct gactacccca
    14221 actcctgccc cggaagcgcc tgaacccacc tccaccccag cccccgcgac caccgacttc
    14281 tacccactgg aaagctacac tacggctttc aacaccggcg atgcttacta cgagcttgtc
    14341 ccatatcccg gtcatgccct gctgctgaaa accgactacg ccaccgccgc ccaaaccgtc
    14401 tgctgcacca tgccaaactg cgcccacgac agtgaagcct gcccggccta tttcccgggg
    14461 cgggcgcggc ggtacacagt ttttacggca ggagataccc tctatgtcta tcacacactg
    14521 ttctaccgca atacccaaac ttgggagaaa tactttgacg agtacggcaa gccccagtta
    14581 gaacacccgg accagagctt cgagggcttg accgccgatg agatcacggc ggatttctac
    14641 ggccgctgga ccgagcagac caccccgccc tgcctgtaca ccgtccaccc gggcggcggc
    14701 aaggtgcgca ccgacctgcc cgaggaagtg cgcgactacg tgatggccta ctgcgacggc
    14761 gcggcggtgt acggcgcagc gtggaacgat gagaacggca ccaccgccac cggctgccgc
    14821 atcagccttg ccgacggtgc ggtacaaacc ttcccgctgc tgacgcagga gcagtttttg
    14881 gatgtctgtg gtgacgcgct ggtgacgtcg cacatcgtta cagatgcgcc cctgcccact
    14941 gaccgggagc agtaccaggc tgccatccag agtgccgaga cggagtatga cctctatgac
    15001 ccgaacgacg gtacccgtac ccggctggct gcctaccctt acgacacgtc ggctttgctg
    15061 ggcgtgtacg gcggcaaggc gtattttgaa aagcgccagc cgatacccta cgccgcgtat
    15121 gagcctgtgg ccgtggagac actggacata gcgaccggca cgaccgagac aatatggacg
    15181 cccgaacagg gggcacagga gctgccgctg ggcgatgccg aaggccacag cctgcgaatg
    15241 agcgagggca acgtgattgt cggccagcac accagcctgc ccgttgattt ccgcgctgcc
    15301 gccgccgatg gccgctggct ggtaatgacc ggcaaggatg agacgaccaa ccgtgagaac
    15361 tacgccctgg tggagccaga gcagtttatg gccgggagcg ccgagcagga aaccgtgaca
    15421 atgtggcagg ggtaacgtcg caaagccctc gcaaaaaggc tacccttcca ggggaactgg
    15481 ccgagtttac gaggcctgag gggctttgca gccttgccat cacagctcac atcaaaaaaa
    15541 ggaggttccc atgaaaaaac tcatccccct catcctcacc gccgccctca cccttgccgc
    15601 ctgtgcatct gccccggaca cagcgtccac ccctctcgca aactcgaccc cggaaccgac
    15661 cgcagcaact acccctgccc caaccacagc ccccttctac ccggtggcca actcgtacaa
    15721 caatggcgac acctactatg cctttgtcca ccgcggcgag gacagtctgc tgctaaaaac
    15781 cgactatgcc gcagctacgc aaaccgtcaa ctgcgcggtt cccggctgtg cccatgacag
    15841 cgacgcctgc cccgcctatt tcacagacga ccccggtcgc aacttggtca tcacggacga
    15901 cccgctgcgc gtctgccatg tcgaggacag aggctgcctc gtgcaaatct acaccgtcga
    15961 ccccgccgag ggcaaggcca tgcaggaaat caatggggtg ggcaactgcg atattgccta
    16021 ctgcgatggc acggcgctgt acggcataga caagacggtg ctgccgagtg aggcaacgcc
    16081 tgcctgccgc atcgaccttg ccagtgggca gcttgacagg tttaccatgc tgccgtcgga
    16141 actgatgctg ggctgctatg acgacggcct gctgacggtc cattacgtga ccgacgcgcc
    16201 gctgcccaaa aacggcgaag agtatgccgc cgccgtccag agcgcgacgc ttgaatttga
    16261 ctgctgggac ccgcgcacag gcacacgcac caagctggcc gagcgcctct acaaccccac
    16321 agacgcgcgg ttcagcggct atgatggcac ggcaaacggc aagctttact ttgtggatta
    16381 tctcccgcag agcgatggca ccgatatcaa actgagcctg actgtcgttg acccagccat
    16441 gggcacagag gaaaaaatgt gggatacgtg gccggatgag cacttcgggc tgtccatagg
    16501 gcaaagaata ctgcccgtgc acggcgacca gagcacgcgg tggcttggga tgtgctactc
    16561 aaacagcgga acaaaccgcg aaatccccta ttttatggat ttggaaacgg gcaagattaa
    16621 agaggtaacg caaaaacgag agagcctgtc ctatgacaga agagtcaccg ttttggccca
    16681 aaccaacgat ggccgctggc ttatcgagac tgcggccgac gagcagcgaa gttgcctcgg
    16741 catggcactg atcaccccgg agcagctgct ggcgggcagc actgactggc aggaaataac
    16801 aatgtggcag ggctgatgca atacgaccag ccgtatgccc ttgtataatt atgcgcgaaa
    16861 acgctgattt gtccacctat taaacacaca ggtccgccaa cagaaaacaa aattttgaaa
    16921 agtaaacaaa aaactcttga aaaatttctt ttgtggtggt aacatgaagg cggtgtttga
    16981 atttatataa aggagtgttc tacttatggc aattcagaat gcttacctgc agggtgtata
    17041 tgagggcctt gcaaagcgta accctgagca gaaggagttt ttgcaggcgg tcgaggaagt
    17101 cctcgaaagc ctggagccgg tcgttgctgc tcatcccgag tatgagaagg ccggcctgat
    17161 cgagcgcctg gtcgagcccg agcgcatcat catgttccgt gtgccctggg tcgatgacgc
    17221 tggcaaggtc caggtcaacc gcggctaccg tgtgcagttc aactctgcca tcggtcccta
    17281 caaaggcggc ctgcgtttcc acccctctgt caacctgtct atcctgaagt tcctgggctt
    17341 tgagcagtgc ttcaagaaca gcctgaccgg cctgcccatg ggcggcggca agggcggttc
    17401 cgacttcgac ccccacggca agtctgatgc cgaggtcatg cgtttctgcc agagctttat
    17461 gaccgagctg taccgtcata tcggccccga caccgacgtg cccgccggtg atattggcgt
    17521 tggcggccgt gaggtcggct acctgtttgg ccagtacaag cgcctgcaga atgagtacac
    17581 cggcgtcctg accggcaagg gcatcccctt cggcggctct ctcgcccgta ccgaggccac
    17641 cggctacggc ctgtgctact tcaccaagga gatgctggcc gacgccggca agagctttga
    17701 gggccagcgc gttgtgatct ccggctccgg caatgtggcc acctacgcca accagaaggc
    17761 cacccagttg ggcggcaagg tcattgccat gtctgactcc aacggctaca tcgtggacga
    17821 gaacggcatt gactataagg tcatcaagga gatcaaagag gtcaagcgtg cccgcatcaa
    17881 gacctatctg gagtatgtcc cgactgctaa gtacgtcgag ggcagccagg gtatctggac
    17941 ggttccctgc gatatcgccc tgccctgcgc cacccagaac gagctgcgcc tggacggtgc
    18001 caaggctctg gttgccaatg gctgctactg cgtagccgag ggtgccaata tgccttccac
    18061 ccccgaggcc atcgcttacc tgcagagcca cggcatcctg tttgcccccg ccaaggcttc
    18121 caacgcgggt ggtgttgcca cctccggcct ggagatgagc cagaacagcc tgcgcctgtc
    18181 ctggaccttc gaggaggtcg atgagcgtct gcacaacatc atggtcaaca tctacaagac
    18241 tgccgccacc accgccgaga agtacggcat gaagggcaac ctggttgccg gtgccaacat
    18301 cgccggtttc gagaagatcg cctccgccat gatgagccag ggcattgtct gattggattt
    18361 gagcagtcag ccataaaaag gcacaaactt ttttcgtaaa acctattgac acacacgccg
    18421 tgctgtggta ttataatcaa gcaccgcaaa acagcgtgtg tgtcatatat cgcggagtag
    18481 agcagatggt agctcgtcgg gctcataacc cggaggtcgc aggttcaagt cctgtctccg
    18541 caaccaacaa aagctcgttg aaacgacaag tttcaacgag cttttttcta ctttatgaca
    18601 aaagtggtaa cgatctgata actttgaccc caaaatgacc ccaaaacggc aaaatgactg
    18661 cgcccggacg gctgaatttt tcaaccgtcc gggcgctttt tgtggataaa ttttgcggag
    18721 ttggcaggta aaaatacgag cggtcagtgt agtcagcttg ccttgacgag taccaacacg
    18781 ctgttgtttg gcacgacaat ctgttcctgt ttggtgggaa tgggcgggtt cttgtattgg
    18841 cgcagggctt tttcgatttg ctgcttgact tcgagcaggg cagcatccaa tgtaggggtc
    18901 tgcacttcaa tttttggaaa gtcaggaaca tgggcggtgc agccgtccgg ggataaggtg
    18961 cagagtacag gatatcccga acaaactgtc catctcaggg cgtttgttgt aaccttatac
    19021 tatcgcaggt tcaggcggga aaggcaatag aaattcttac acaaaaacag caataattgt
    19081 atgtcttgcg caaaatgtgt gcgcttttca taaacggaaa aatttgcagg ttcctcataa
    19141 aatcggacat gggagaaagc gcgcaactgc gctatgataa gagaaaccca ctgccatgaa
    19201 aagcccgatt tttaaagaga ggatgaaaaa ccatgagaaa agcaaagcgc agatgccaac
    19261 agctggttgc agcggcaatg tgcgtcggta tgctggccgg tatggtcccc acacaggtct
    19321 ttgccgagga agcacagccg gctgcagcaa tcgcgcagca ggagcaggcc cctgcaccgt
    19381 ccgccaacga gacggaaacc cctgccccga ctgaggaacc ggcggccgag cagacccctg
    19441 ccccgactga ggaaccggcg gccgagcaga cccctgcccc gaccgaggaa ccggcggccg
    19501 agcagacccc tgccccgact gaggaaccgg cggctaagca gacccccgcc ccggctgagg
    19561 aaccggcggc tgagcagacc cccgccccgg cggaagagcc tgcggccgag gagaccgctg
    19621 cacaagctgc cgaggtgatg agcgctgcgg tgcaggactc cgagaatgag aacttcggca
    19681 aggtgctgga ggatcaagac cttgttgcgg tgcgcagcgg cgcttctctg gtggagtcgc
    19741 tgtccaccgg caagcaggtc aaggtaaccg ctgaatggat cagcggcagc aactccgctg
    19801 tggacggcgg cggtgccctg caggaggggg acgccttctt caagcgcagc cgcttcactc
    19861 tgtatgcgaa tgtcaaattc gatgctgcgc atgacaacac ctcggcgctg cttgtcggca
    19921 gcgatgccgg tgcaaacttc cgcattgtgc cctgcaagac ggacggcaca gctgtgctga
    19981 aggtcaacaa cggtacggag cacaagctct ccaaggcgat gaccgccgga gaatggaacg
    20041 ccctggccct tgtctatgcc gagaatgaca ccgagggcac ggtggccgtc tatctgaacg
    20101 gcgaggaggt cctgcctgcc accggcatcg gcttcaagct cagcgagaag tccggcatcg
    20161 tgggcgccgt gggcgccacc ttcggcaccg gctttatgcg caccggcctg tatgacgagc
    20221 tggtcgtgac cggtgcggcg gacgccgagg ccgccaaggt cgagactgcc gcccgcaagg
    20281 cggcggccga tgcggttgtc gcggtggacg gcgttgtcac cgtcaccggt gcggaggtcg
    20341 agcaggccgc ccagaatgtc aacggctgga cctataaggg gctcggcatg ctgaacggca
    20401 actccaccag caacctgctg cttgactaca aggccgaaag ccctgacgcc tactgggcca
    20461 tgatgcgcta tctgttcggc ggtgactacc cgctgttcag caacatcaag atggagatgg
    20521 gcaacgacgg caacaactct accggtgccg aggcctgcac catgcggtac gaggacgagg
    20581 aggcagatgc ctcccgctcg cccggctttg tcatggccgc cgatgccaag acgatcaacc
    20641 cgaatgtcaa ggtgtcgatc ctgcgctggg gctgcccgaa ctgggtcaac gcctacaaag
    20701 gcacagactg gtatgccgcc aactacaaat ggtacaagga aacgatcttt gacgcctacg
    20761 agaagtacgg ctatgtcgtg gattacatca accccgacac gaacgagacc ggcaatcctg
    20821 acagcgggat catcaaatat ttcgccaacg ccattgccaa cgagacggat ttcccggaat
    20881 attttaccga ggaggccaag caggcctacc gcagcatcaa gatcgtggcc tcggatgaga
    20941 acaagggcct gaagatcgta cccatgatgc gcggtgacaa ggacctgtat gacgcggtgg
    21001 atgccatcgg cttccactac cgcaccaacg ccaccgatga ctatatcaag atggccgatg
    21061 tggatgacaa ggaggtctgg tacagcgagg gctgtgcgac cttcggttat tccgagctgc
    21121 aggagaacaa gaccgccgag tacggtgcag gcaccatcgg cggctaccaa agcccgctgg
    21181 cgctgctgga cagcttcccc aacgcctttg tcggctcccg ccggacgatg tacatgttcc
    21241 agccggccat cggcgccttc tacgagggca tccagtacgg ccataaggag ctgctctccg
    21301 cccgcgaccc gtggagcggc tatatccact atgaccctgt gctttacatg ctgggccata
    21361 tcaccaagtt tgccaagacc ggctgggaga atgcaagcaa caccgccggt atctggcgcg
    21421 tgctgccggg cgctaccttt gcgtccttcg gtgccagctc gaacgagcat gccaccgcag
    21481 gcattgacgg aaacgccagc tatatgacgc tggcagcacc cgacaagcag aacttctcca
    21541 cggtgttcgt caacaacacc cgcaacgaaa agacctttgc cgtcaagctg tccgacatgg
    21601 cgctgaaggc cgaggccctg cacatctgga cgaccgcgac cgacagctat atgcagaagg
    21661 gcgaggacgc caagatcgag ggcggcatcg cctatgtgac cgtaccggcc tacagtgttg
    21721 tgaccgccac tacgctggat actgagccgg agcgcttccc cactgaggac ggcagcggcg
    21781 attacatcca gactgcgacc cgcgcggtgc tggacaccga tgcgaccggc aagaaggctg
    21841 atacggccga tgactacctc tacgccgata acttcgacta taaggaggag gaactcaact
    21901 acatcgtgga ccgcggcaat gagccccgct acatgctgga cacccacggc gcatggatcg
    21961 tggagaacgg ccgcttaaag caggagaacg ccgccggcgt gacccagtgg aacggcggtg
    22021 acccggctac catcgtgggg gactggcgct ggatggacta cagcgcttcg gtggatatgg
    22081 agctgcccaa cgccgatgcc aaccggtatg agcggctgac catccgtgcg cagaccggca
    22141 tgaactggaa caacagcggc tacacgctgg agctcaacgg cgcgggcagc tggaagctgt
    22201 tccgcatcgg ctccgctgtg gccagcggca gtgttgccaa ggatgatgct ggcaagtaca
    22261 atgtaaagct catcggcttg ggcgacaccg tctatgccta tatcaacggc acgcttgtca
    22321 ccagctacac cgatgcaaat ccgatgctct ccggccgtgt gaagatcagc tccaactgga
    22381 cgcaggtcta tgcggacaat ctggaaatca ggaccgtcaa gggcggcatt ccctacgcca
    22441 ctgccatgat cgatggtcag gacgatggcg ttgtctatga gggtgcatgg gccatcaaca
    22501 acccgggcgg cggcagcgct gacaactggt accgcacgat gtctgaggcc agcaccgccg
    22561 gtgcggcgtt cacctttgcg gtggacggca gcggctttgc catcatgggc agcaacaacg
    22621 gctccgctgt gatcgatgtc tatgtggacg gcgtgatgaa ggttgaaaac gccgccacca
    22681 aggccgccca cacccgcggt gaggcgtaca ttctgtccga ccttgccgcc ggcaagcaca
    22741 ctgtcaaggt tgtgctgaaa tccggcacgc tcaaggtgga cgcgctgaac accatcggca
    22801 accgcctgcc tgccgccgag ggtgcagtta ccgaggtgct gaccaagctg cctgcgctgg
    22861 aaagctatgt caccggcagc ggcatcggca gcctgcccgc cgcggtcgag gtcaaaaaga
    22921 cggacggcac caccgcgacc ctgcctgtgg attggaatgt ggatatgaac gcactgaatg
    22981 ccaatgccta cggtgcggcc gagatccagg gcaccgtcaa aggggccgtc aacccgctgg
    23041 gcgagaagct gactgtcagt gtcaagatcg gcgaagttat ccccggcgat accgtggtat
    23101 tcatcgacag tgtggccggt gatccggcag ccgtcagcgg cggcacgacc gagagctata
    23161 cgctgtacaa aaaggctctg ggcagcaagc tgctgaatga agcctttgac cagcgcaaga
    23221 ccgccgataa tgactggggt cttgtcgata cggatgccgg taccaagggc tacagctcca
    23281 ccgctgacaa gaccgcgacc ggcatctacg gccgcgaaaa taaagcgggc gagaccctga
    23341 cctacgccct gaccctgcct gcaggcagct acatcctgac gagcgcccac catgaatggt
    23401 ggggccagaa ccgcccgatg accgccgtgg tcaccgatgc cgagggcaac gccctgagcg
    23461 agaccgcgac cctgaacctg aacggcagca gcggggatat cctcaacaag gctgtgttca
    23521 agttggacgg tcaaaaaacc gtttactaca cggtgaccgc caccggcagc caggcacctg
    23581 tcatcagctg gctgggcgtg cagcgtcagc ccgccgagac gaaggtcgtt gtgacgaagg
    23641 gcctgaccag cgtgccggag gcactgcaaa atacagcgtt tactacaacc gaaaaaattg
    23701 agaaagagct ggtaaagcag ctctccgatg agatcagcgg ctgcaaggtg agcatctacg
    23761 atgtgaagct gatgctggat ttcggtgacg gtagcctgat cgaagccgag gaaaacagct
    23821 tccctgcagg gggcatggat gtctggttcg acctgcccga tggtgtgacc gcagagaatt
    23881 tcagccagtt cgatattgaa gtggcgcaca tgctctgcac ccgcgaccat gccggtgagg
    23941 ttgagctgct gaaagacccg gagctggacg ctgcgaacgg caagctcaag gtgcatgtag
    24001 gctccttctc tccctttgcc atcggctgga aggccaagcc tgcctccgga ccggtggtga
    24061 cgccgggcac cggcagcacc ggcacgaccc ccggcggcag caccggtagc aacaccggca
    24121 gcaacacgaa tgccggcaac agcagcaata ccgcagcagg caatgcgggc ggcaccgccg
    24181 ctgctgcaag cggtaccgcc aagcctgcag gcgcagccga taacaaggct gcggtgcctg
    24241 ccaagagcgg gctgattccc cagacctcgg acagcttccc gctgctcccg gtcgcaggca
    24301 ttgcactggt cagtctggca gcggtcggcc tgctggccct gctgaaaaag aaaaagcacg
    24361 aaacgcgctg agcctctgac gatataagat cgtgtttgca cagtccccgc ccggaaacgg
    24421 gcggggattt gttgctgcta tgacaaaaag ccgcacacac tgcgaggtgt gtgcggcttt
    24481 ttgcggcttg gcgggggtta cggtgcctcg gtgccccatg cctcaagctg ggtcagcgcg
    24541 gggaacgggg acgggtcctc ggcctttttc agctcaaaca gcttcaggct ttttgcggtg
    24601 cgcggggtga tcgcaaaggt ctgcggggcg gcggtcttgg tcagctgcaa aacctcgcgg
    24661 ctgccgtcat caaacgccac ggtcgcctgc gtccagtagc tgtcgtgcgg gaaatccgcg
    24721 cgcagcgtca ggcgcagctc gtccacgatc acggcgcggc cgaaatccag cgtcagcgcg
    24781 gcattggggt cgcggttaat gccccagctc tggtacggcc aggtgccgtg ggcgtcattc
    24841 tcatacacgc cgtccacggc gttgcgggcg gcaaacacgg cctcgccgcg ggtctccaca
    24901 ttggcggacg catgggggaa gaagccggta tcaccgtgtt cgtcataggg gttgaacgca
    24961 aggttgcgcc gcgccttgat ctccgcctcg gtggcaaagc gggcgcggat gatgtggcag
    25021 cacccggaaa agcttttggg gctgtaggtg atggcctgct cgccgaacgg aatgtggaag
    25081 ttgatctccc gcttgacgac atagacgagc gccggggcca tcgtgtcctc aaactggatc
    25141 acgatatact gcccgggtgt gccgatctct tccactttct gaatagcctt gataggatca
    25201 tca
//
LOCUS       DBMDSQ010000002        23682 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_742_length_23682_cov_2.425088, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000002 DBMDSQ010000000
VERSION     DBMDSQ010000002.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 23682)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 23682)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23682
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_742_length_23682_cov_2.425088"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..350
                     /locus_tag="ACZPAX_00110"
     CDS             <1..350
                     /locus_tag="ACZPAX_00110"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007048402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR1112330.1"
                     /translation="VRMAVFSNKADEFARAVVARYFDASLFEIVRGALPDVPTKPAPE
                     GTRALMAALGVKPDGGVLYVGDSNVDVETAHNAGLPCCGALWGFRTREELTEAGAEYL
                     AEDAAALEKVILG"
     gene            866..2065
                     /locus_tag="ACZPAX_00115"
     CDS             866..2065
                     /locus_tag="ACZPAX_00115"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007048403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HIR1112331.1"
                     /translation="MIAQHYKDMLGAKSVIRQISEWSTARGKEIGYENVFDYSLGNPS
                     VPCPPQFTETCEDLLKNTDPVQLHGYTPTLTLPSTRKAVAESLNRRFGMDYTADHIFM
                     TTGAAGALAHAMRCVAVPGQNIVTFAPFFPEYKPYVEGAGLTLRVTPPRCADFQIDFD
                     AFAGLVDENTAAVLINSPNNPSGTAYSEATLRQLADFLTEASAKYGHHIFLLSDEPYR
                     EIAFGGAVTPYPAKFYDDTLTCYSFSKSLSIPGERMGYVAANPRCEGAKYIVPMCGQI
                     SRGTGHNCPSSLIQMAVERCLDVTSDLSVYETNMNLIYDELKALGFTVVKPDGTFYIF
                     PKALEDDAKVFCQKALQYDLALVPGDSFGCPGYFRMAYCIETEKVRRSFAALEKFVAE
                     EYGVTKH"
     gene            2075..3034
                     /locus_tag="ACZPAX_00120"
     CDS             2075..3034
                     /locus_tag="ACZPAX_00120"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HIR1112332.1"
                     /translation="MSILRRILPLLLVVTLLCSCVEMPNAAPDPTPVPTAQPTATPES
                     TPAPTPEPTMVPAMASATNNATKDAPDVKDPATWDDNAAETLASLVEQFGRWGLTLDA
                     KADYPYLLAVNREAGVVTVYTVDTATGQYAVPYMAMVCSAGNDTPTGYYATPVSYSWR
                     LLMGPSYGQYATRIYDGYLFHSVPYYSQHKDDVEYDEFNKLGTVASLGCIRLAVVDVK
                     WIYDNCPLGTPVVIYNDKDPGPMGKPGTIYTDPDDEDKRGWDPTDPDPVNPWDDSFES
                     GTAIRSQAAWDQWENEHENWMSSLTPTDLQGWSTDSKIEGTRG"
     gene            complement(3199..4176)
                     /locus_tag="ACZPAX_00125"
     CDS             complement(3199..4176)
                     /locus_tag="ACZPAX_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005921643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family putative permease subunit"
                     /protein_id="HIR1112333.1"
                     /translation="MPKQIPVYLFVGQLESGKTKFIQETMEDPNFDSGDKTLLLVCEE
                     GEVEYDPSKFAFGGVHVAQIEDKSELTQENLTALEKKSGCGRVIIEYNGMWLVQELYD
                     AMPDNWLVYQCLATADGTTIKTYAGDSAMRSLLLDKIRGSELLVVNRAEAVNDDESRQ
                     LIHKLVRQASRRCDIAYEFKDGSVAYDDIPDPLPFDIDAPVIEIPEEFFGVWYMDCMD
                     DPKKYDGKTVKYLAQVCQTPQAGKGAFVPGRFAMTCCVQDIQFVGMPCKYDDYKKLEQ
                     RSWINITAKVNVKYHPIYKGQTPDSTGPVLTAVTVEPGEKPAQDVVMFS"
     gene            complement(4426..5652)
                     /locus_tag="ACZPAX_00130"
     CDS             complement(4426..5652)
                     /locus_tag="ACZPAX_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015537192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CobW family GTP-binding protein"
                     /protein_id="HIR1112334.1"
                     /translation="MAENKTKIDIFSGFLGAGKTTLIKKLIKEAFVGEKIVLIENEFG
                     EIGIDGGFMREAGIQVNELNSGCICCSLVGDFREALKKVVETYHPDRILIEPSGVGKL
                     SDVTRAVEGVAESLPVILNSFVTVADVNKVKMYMKNFGEFYDDQVSHASCIILSRTGN
                     ASEEKIAAAVALLAEKNPTATIVTTDWTALTGAQIVSVMDGKRDLVAELLTEARAANV
                     AHADEDEHEHHHHHHHHDEDDHDEHECCHHHHHDDDDDDDEHDHECCHHDHDEDEHDH
                     ECCHHHHDDDEDEHEHEHHHEHHYDEHGVCSCGHHHHHHDHDADEVFTSWGKETAHKY
                     SREDLDKALHALDEGAYGMILRAKGIVDGGDTWYEFDMVPGEHEIRTCGPDVTGKLCV
                     IGSELKEHEIAELFHV"
     gene            5989..7776
                     /locus_tag="ACZPAX_00135"
     CDS             5989..7776
                     /locus_tag="ACZPAX_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015566070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase family protein P"
                     /protein_id="HIR1112335.1"
                     /translation="MTTNEKIAALRAAAKAAGANGVLIMTSDPHCSEYLPGYYNALPW
                     FSGFIGENSTLVVTLSGSALWCDGRFYVQADKQLAGSEIECMHAGSAGVPTVAEYLEN
                     HFGAGDTLLLDGSCVPATIAKEYIDALAKKGASLKSQDVASPIWDATGERPALPDTPC
                     ELLTPAQTGATAADRIAMVRAELQKAGATALAVTGLDCVGWLLNLRARDLPCTPLAVA
                     YALVTMDACTLFIAPGRLSDADAATLAESGVALRGYNELIDAVHALPAEEVFLVDEKA
                     TNYALYEALTAHKTVVGADPIFALKGIKNEVELKNIRECHIRDGIAVVRFQMDLEKAL
                     AEGKTLTEIDIDTMLQKRRAEMPGYFEDSFSTIAAYGTNAAMMHYHAEGDVNSVIEPR
                     GFLLVDNGGQYDCGTTDITRTYPVGPLTDNERRYYTWVLQSHIDMARAVFLDYCTGFA
                     LDTFARGPVWAHKVNYRCGTGHGVGFISGVHEGPQSLRPNNPVIFKPGMTITDEPGIY
                     ETDEVGIRIENELECVDLGDNQYGHWLGFAPLTLVPIATEPVLVDELSRDQINWLNDY
                     HAHVYEMLSPRLHEDEKVWLKEKCAPIGR"
     gene            7858..8004
                     /gene="scfA"
                     /locus_tag="ACZPAX_00140"
     CDS             7858..8004
                     /gene="scfA"
                     /locus_tag="ACZPAX_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015563834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="six-cysteine ranthipeptide SCIFF"
                     /protein_id="HIR1112336.1"
                     /translation="MKHIQTLETRDMAKSLLNGGCGECQTSCQSACKTSCGIANQPCE
                     KTDK"
     gene            8179..9639
                     /gene="scfB"
                     /locus_tag="ACZPAX_00145"
     CDS             8179..9639
                     /gene="scfB"
                     /locus_tag="ACZPAX_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0031179 - peptide modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioether cross-link-forming SCIFF peptide
                     maturase"
                     /protein_id="HIR1112337.1"
                     /translation="MIHQYKLSGYNIVLDVYSGSVHAVDDVAYDAIALIDQGSSREQA
                     AAALAKKYAGRADVTESDINDVLDDIDELTAAGQLFAPDAYADHAFDFKNRSNVVKAL
                     CLHVAHTCNLNCSYCFAAQGKFHGEAGLMSFETGKRALDFLVEHSGTRRNLEVDFFGG
                     EPLMNFEVCKQLVAYARSIEKEHNKNFRFTMTTNGIGITDEVIDWCNKECHNVVLSLD
                     GRKEVNDRFRVDLAGNGSYDRIVPKFQKLVKARGGQGYYMRGTFTHHNVDFTKDLFHM
                     ADDLGFTELSMEPVVAPPDSPEALTEEDLPKLFDQYELLANDMLRRQKAGKPITFYHY
                     ILDLKHGPCIYKRISGCGSGTEYMAVTPWGDLYPCHQFVGDPNYKLGNVWDGVTNTAL
                     RDEFKLCNVYARPDCKDCWARLYCAGGCAANALHATGDIHGTYEYGCKVFRKRMECAL
                     MMQVAQRLDPELAKNAVKFESDCDGCGEGNDGACEK"
     gene            9763..10971
                     /locus_tag="ACZPAX_00150"
     CDS             9763..10971
                     /locus_tag="ACZPAX_00150"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007491704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HIR1112338.1"
                     /translation="MPYDFTTIYDRHGKDALAVEGLGKPGFAPPAPKNGFSAIPMWVA
                     DMNFATVPTIPAAIIARASHPLYGYFLTQDAYYDAIIGWQRDRNGVEGLTKDAIGYEN
                     GVLGGVASALGCLAAPGDAVLLHSPTYIGFTHTLEDNGYRIVHSPLVQDENGTWRMDY
                     ADMDRKCKENHIHAAIFCSPHNPCGRVWERWEIEKAMDVYRENGVTVISDEIWSDIIL
                     GGHKHIPTQSVSEDASQRTIALYAPSKTFNLAGLIGSYHIIYNKTLRDRVAARSEKTH
                     YNEMNVLSMHALLGAYTPEGRAWVDELCAVLTENVDYACRYIAEHFPGVRVAKPEGTY
                     LLFLDCTDWCAAHGKTIDEVEQAGWDVGVAWQDGRMFHGPCAIRVNLALPKSLVVEAF
                     ERLDKYVFNA"
     gene            complement(10961..12373)
                     /locus_tag="ACZPAX_00155"
     CDS             complement(10961..12373)
                     /locus_tag="ACZPAX_00155"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013444.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR1112339.1"
                     /translation="MKNQTLQQQLQALSVARYPLHMPGHKRRVRPAPGLDCYAWDLTE
                     IDGADDLHDADGILADAMARTAALYGARRCWYLVGGSTVGLLAGIRALAPFGSTVIAA
                     RNCHKAVYHAIELGHLTVHYLTPPADPQFGVYGSVRPADVAAALDAEPGAKCVILTSP
                     TYEGVLSDVRSIAEICHARGVTLLVDEAHGAHYLPFAAQYGWQGGAVAAGADLVVQSA
                     HKTLPSLTQTAFLQLNGDLADPAEVERQLDVFETSSPSYPLMASLDGCTRWLAEEGSA
                     AFAAWRARLDRFDAAVKDLQKTKVMCFGRDALDAHPCFFAHDSGKILLQIGTEGAAYL
                     RADGFEPEMVCGPNVLAMTSPCDEENALDRLAAVLHAWEEQAVPPAPAAHILPPPGAA
                     RCTIAEALLRPAHTVPLADAAGETAAEYIWAYPPGVPLLAPGEEVTPEFLAACRELEA
                     AGTRLKHIGGSGKMELRVMR"
     gene            complement(12542..15037)
                     /locus_tag="ACZPAX_00160"
     CDS             complement(12542..15037)
                     /locus_tag="ACZPAX_00160"
                     /inference="COORDINATES: protein motif:HMM:NF024867.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR1112340.1"
                     /translation="MKPQKLILTAFGPYAGRTELDFSAFGGNGLFLIGGDTGAGKTAL
                     FDAITFALYGETSGENRKTTMLRSDFAAPDAETSVELTFTHRGRTYTVRRWPDQQRTA
                     KRGSGMVKVPAKAELIREPDEPVSGASAVTDAVVKLLGIDAKQFAQVSMLAQNDFTKL
                     LNAPSAERAAILRRIFDTADHQRVGQAAVDHARKADEECKRLDDVLLMHMGTLLGDGA
                     DEETAEDLREMQAERDPFTAGAAAELGKILLEADDANEKRQTNVMKDLDEKIARGDAG
                     LKIAEERAARRRQLAGLVAEEQRAADVEHQTDLVMTDLEKRTASLKENIAKNEADRAA
                     LSQAEADLLKTDHRIELAEGLSADCKRLLQNEADAEKAQHDAAARQAEYVAAQAALDA
                     AEEEYSTMQRQVNANRAGLLAKDLKNGKPCPVCGSTRHPKIAALPKDHITEKQLEERE
                     KELTAQRRSTAAASRTAGDAAAHANELQTALQRDVDGFFARRGDRYTGKPAAELTADE
                     LRDALTAQQKSLADGLRGLQADHLKLKQKTDRAKNLAKQAEILNRQLADLEKQSFAAN
                     RRAANAKAGHAAATARVQQMRETMPKRDNPDALTQLQDALARLRRDRAAAMEARDAAV
                     HRLHTNRAAIDALKKAMKESAVAREKRAMWDNLSKTINGNLAGKIKLPFEQYVQAFYF
                     DGVVEAANLRFTRMTDGQYRLLRRKSEAVGGKTALDLDVFDAYTGKTRPVGSLSGGES
                     FMAALSLALGISDTIQQNAGGVVIETLFIDEGFGSLDSDSLEKAVDTLAGLAGGDKLI
                     GVISHVEALQDRLTRQIHVTKTRAGSKAEIELS"
     gene            complement(15034..16167)
                     /locus_tag="ACZPAX_00165"
     CDS             complement(15034..16167)
                     /locus_tag="ACZPAX_00165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0004529 - DNA exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HIR1112341.1"
                     /translation="MKFLHLADLHLGKRVNGFDMLEDQRFILDQILTLCDKHGAEAVV
                     LAGDIYDTPVPPAAACTLLDWFLTQLATRKIPVLAVSGNHDSAERLDFASGLLAEQGV
                     YIAGRFTGAPRQVVLHDRHGAIEFTLLPFVRAATVRHYLPDADITDYDSAVGAALAVC
                     EPAAPRRVLVAHQMVISGVCPPQLSGSETAPLTVGTVDSIDAARFAGFCYAALGHIHR
                     AQRVGIDAVRYAGSPLCYHLDECGAQKSVTLVRIGKRGVEKIEPLPLMPRRAMRHLTG
                     PLAKLVEHPTDTEDYIWATLTDPTPLPDAMAQLRAAYPNAMRLDYQPQGAAALPADMT
                     QAVRGKPFAELFQDFFTKMNGRPLTTEEVLAVKKLREEASKIE"
     gene            complement(16309..16809)
                     /locus_tag="ACZPAX_00170"
     CDS             complement(16309..16809)
                     /locus_tag="ACZPAX_00170"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR1112342.1"
                     /translation="MNPTFKQVTKTRYVELVAELAQEIFVQHYTKLTPKVAAALADKY
                     QSDILTDDEIHSGVINYFLIYLGTEPVGYFALDLGPAGAMCLSRLFLKEKARGQGIGR
                     SIVAYAQKLAEGDGRSRLYMKVWARDLKAEGFAKKCRFRKAETAPMEVLPGTTLDITT
                     WEKLWR"
     gene            complement(16826..17158)
                     /locus_tag="ACZPAX_00175"
     CDS             complement(16826..17158)
                     /locus_tag="ACZPAX_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015537827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HIR1112343.1"
                     /translation="MTKWKKNLIFAAVVLAAAAVLWLATRSGGSGKTAVLRYGDPQVE
                     QRIDLRKNADYDIDTGLYTIHLHVENGGIAFVNSPCPDHTCEGFGVLKNQGDWAACMP
                     AKASLTIE"
     gene            complement(17155..18045)
                     /locus_tag="ACZPAX_00180"
     CDS             complement(17155..18045)
                     /locus_tag="ACZPAX_00180"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015537488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity
                     producing 5'-phosphomonoesters, hydrolytic mechanism
                     [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HIR1112344.1"
                     /translation="MLKVTLLGTGGTQPLPERALAAAAVSVGGHNILLDCGEGTQVGL
                     RRYGVSAYRLSAVLLTHYHGDHILGLPGLLQTLGSLNRTEPLTIYGPRGIEAVSRAIM
                     ALAGPQAYPVRWQQADAPFTVNELQITPFPLTHRVPCCGYALALPRAGKFDPARARAA
                     GIPIEYWGTLQKGENVGGFTPEQVLGAPRRGLKVVYATDSRPCPAIVEAARDADLLCM
                     DATYADDADLDKAKLYGHATCRETGETAAAAQVRRLWLTHYSAAVSDTAPGLAAAQAA
                     CPAAVAGYDGLTEELEFDKT"
     gene            18173..18661
                     /locus_tag="ACZPAX_00185"
     CDS             18173..18661
                     /locus_tag="ACZPAX_00185"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR1112345.1"
                     /translation="MTERELDILLREYSGAVRAVCRAILPGHPQDAEEAEADTFYKLW
                     RGARLPADEAHRRRLVVLTARQCAIDRYRALLRRGETLPLDDRDDAELAVALESHLES
                     RELMAQIMALPPPDGELFLRRYLYGETAAQLGARFAMPAATVRTRLHRARLALQKLLK
                     EG"
     gene            18665..19723
                     /locus_tag="ACZPAX_00190"
     CDS             18665..19723
                     /locus_tag="ACZPAX_00190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1112346.1"
                     /translation="MTELERWMNDHPPLADALTAEESAALRQKVLARRPKPRVLRGWR
                     LAAAAAALCLVAAGAVVLGLFHPMSAANETNALVQKYGEVLDEPLTAEINGRTVSVKA
                     LLRGENTLRVLYDVDNAQGMEPNDLVWDTGAFYLRAADNSGDKRLYIASNAQVGPCYG
                     YAIGSWATRNAVSEGGVLSCYVDIPLTQTATDTRTLYICDLTNHKASAHVKVMLPNMA
                     EAVTLNVKKTIALKGGHWFHYDMTEEEAEQYPDATFDIQEVRIEPLAVSVIGKNHGSL
                     PLGGTSRYDVQSRVKLFRADGTELTYGWDGEFYMGQYGRITKNSGKITLRVDSYDIID
                     PEAVAYVEIDGERFDVNR"
     gene            complement(19810..21000)
                     /locus_tag="ACZPAX_00195"
     CDS             complement(19810..21000)
                     /locus_tag="ACZPAX_00195"
                     /EC_number="7.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007048773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HIR1112347.1"
                     /translation="MGEIFKNIAEIFAKSGWAQIIFTEGGWKYAVMLAVACVLLYLAI
                     VKQFEPLLLLPIGFGMLMTNLPLDGIFHMDIFINETNHIDWALLGSSGGMVDYIYLGV
                     KLGIYPPLIFLGIGTMTDFEPLIARPSSLLLGAAAQLGIFFTFVGAKILGFTNQEAGA
                     IGIIGGADGPTAIYVTTKLAPHLLGSIAVAAYCYMALVPVIQPPIMKVLTTEKERQIV
                     MTAPRRVSKTEKILFPIIVTIIVALTLPDAAILVGCLMMGNLMKESGVVERITKTAGN
                     ELMNIITIFLGFSVGCTTNAATFLNIQTVEIIVLGIIAFGVGTAGGVILGKVMCVVTH
                     GKINPLIGSAGVSAVPMAARVSQKVGQEYNPRNYLLMHAMGPNVAGVIGSAVAAGILI
                     NMFG"
     gene            complement(21013..21423)
                     /locus_tag="ACZPAX_00200"
     CDS             complement(21013..21423)
                     /locus_tag="ACZPAX_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005941465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008948 - oxaloacetate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OadG family transporter subunit"
                     /protein_id="HIR1112348.1"
                     /translation="MPNLALLALEASDGTVVLTSITLVFAMLVALCLIITLEGKIFDN
                     INNKKNGKPAPKAPAAPAAKAAPAAKPAAPAAKADNGSIPGEIIAAISAAVASVCGGN
                     AVIRGIKRVSKPAAGSRRGAWGEAGVREHTTPFM"
     gene            21985..>23682
                     /locus_tag="ACZPAX_00205"
     CDS             21985..>23682
                     /locus_tag="ACZPAX_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_269836823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HIR1112349.1"
                     /translation="MFNSADVTWTLVAAFLVFFMQAGFALCEAGLTRAKNTGNILMKN
                     MMDFCIGTPCYWIVGFGIMFAGSGALIGGFDPFIRGSYDFGTLPVWVYAVFQTVFCAT
                     AATIVSGSMAERTKFSAYCCYSAAISLIVYPISGHWIWGGGWLAQLGFHDFAGSTAVH
                     FVGGVTACLGAWMLGPRIGKYNKAGTPRAIPGHNLTAMALGVFILWFCWFGFNGGSTV
                     SMTGDDTMISAGLICFNTNLAAALATVAALIVSWVRYGKPDVSLTFNGALAGLVAITA
                     GCDVVDPFGAAVIGIVAGVLCIFSVEFFDKIAKIDDPVGAVSVHCVNGCWGTLAVGLF
                     ATEGGLFYGGGFAKLGIQLLGVVSVAAWVLITMYIIFSIIKKTIGLRVSEKEELDGLD
                     IHEHGLTSAYAGFAISDPTYAELDVNENTDLGEDDITKASPEKIAAAVKVTQEALLPA
                     ELDSGMHKVSIIVQLAKFEALKKALNGIGVTGMTVTQVMGCGLQKGSGEKYRGAEVDA
                     TLLPKVKVEVVVSKIPVDKIIDTATKTLYTGHIGDGKIFVYNVAKVVKVRTGEQDYAA
                     "
BASE COUNT         4403 a         7319 c         7459 g         4501 t
ORIGIN      
        1 gggtgcggat ggccgtgttc tccaacaagg cggacgagtt tgcccgcgcg gtggtggccc
       61 ggtattttga cgccagcctg tttgaaatcg tgcgcggtgc actgcccgac gtaccgacaa
      121 agcccgcccc cgagggcacg cgggcgctga tggcagcgct tggcgtaaag cctgacggcg
      181 gcgtgctgta tgtgggtgac agcaatgtgg acgtggagac cgcccacaat gcaggtctgc
      241 cctgctgcgg cgcgctgtgg gggttccgga cccgcgagga gctgaccgaa gccggggccg
      301 aataccttgc cgaggacgcc gccgcactgg aaaaggtgat tttggggtga gggtgcccgg
      361 gagggatgaa tccctcccct acggattgac cgtaaaaggg atttgtcggt aagttgcggg
      421 ccgggcatgc ccggccccta caggggggcc ggaaggttta cggcaaggat tgtagggggc
      481 ggcatccccg acgccccgca ggggagcagc ctttcccgtg tctcatgtag ggcggccagc
      541 cctctggccg ccgtggccgt ttgcggcggc gggaagtacc cgggagggat gaatctctcc
      601 cctacggatt gaccgtaaaa gggatttgtc ggtaagttgc gggccgggca tgcccggccc
      661 ctacaaggtg gccggaaggt ttacggcaag gattgtaggg ggcggcgtcc tcgacgcccc
      721 gcaggggagc agcctttccc gtgtcacatg tagggcggcc agtcctctgg ccgccgtggc
      781 cgtttgcggc ggcgggaagt gcccagtagg gactgccgtt ccccctcccg catctcgaca
      841 aacattttgc aaaggagctg ttattatgat tgcacagcac tataaagata tgctgggggc
      901 caagtcggtt atccgccaaa tttctgagtg gagcaccgcg cggggcaagg aaatcggcta
      961 cgagaatgtc tttgactatt cgctggggaa tccgtccgtc ccctgcccgc cgcagttcac
     1021 cgagacctgc gaagacctgc tgaaaaacac cgacccggtc cagctgcatg gctacacgcc
     1081 gacgctgacg ctgccctcga cccggaaggc cgtggccgag agcctgaacc gccgctttgg
     1141 catggactac accgccgacc atatctttat gaccaccggc gcggcgggtg cgctggccca
     1201 cgccatgcgc tgcgtggctg tgcccgggca gaacatcgtg acctttgccc ccttctttcc
     1261 cgagtacaag ccctatgtcg agggcgcggg cctgacactg cgggtcacac cgccccggtg
     1321 cgcggatttc cagatcgact ttgatgcctt tgccgggctg gtggacgaaa acaccgccgc
     1381 cgtgctcatc aacagcccca acaaccccag cggcaccgcg tacagcgagg cgaccctgcg
     1441 gcagctggcg gactttttga ccgaggcgtc cgccaagtac ggccaccaca tcttcctgct
     1501 gtccgacgag ccgtaccgcg agatcgcgtt cggcggcgcc gtcacaccct accctgccaa
     1561 attctacgat gacacgctga cctgctactc cttctccaag agcttgagca tcccgggcga
     1621 gcgcatgggc tatgtggccg ccaacccccg gtgcgagggg gccaagtaca tcgtgccgat
     1681 gtgcggccaa atttcccgcg ggaccggcca caactgccct tcgtccctca tccagatggc
     1741 cgtggagcgc tgcctggacg tgaccagcga cctgtccgtg tatgagacaa acatgaacct
     1801 gatctacgac gagctgaaag ccctgggctt cacggtcgta aagcccgacg gtacgttcta
     1861 tatcttcccg aaagcgctgg aggacgacgc aaaggtgttc tgccagaagg cgctgcaata
     1921 tgacctggca ctggtccccg gcgacagctt cggctgcccg ggctacttcc gcatggcgta
     1981 ctgcatcgaa accgagaagg ttcgccgcag ctttgccgcg ctggaaaaat tcgtcgccga
     2041 ggagtacggc gtcaccaaac actaaggagc atttatgtcg attttacgcc gtattcttcc
     2101 gcttttgctg gttgtcaccc tgctgtgcag ctgtgtagaa atgcccaacg ccgcaccgga
     2161 ccccacccct gtgcccacgg cacagcccac cgccacgccg gaaagcaccc ccgcccccac
     2221 gccggagccg acgatggtgc ccgccatggc ctccgccacg aacaacgcca ccaaggacgc
     2281 ccccgacgtg aaggaccccg caacctggga tgacaacgcc gccgagacgc tggcgagcct
     2341 ggtggagcag ttcggtcgct ggggcctgac gctggacgcc aaggccgatt acccctatct
     2401 gctggcggtc aaccgtgagg cgggcgtcgt cacggtctac accgtagata ccgccaccgg
     2461 ccagtacgcc gtgccctaca tggccatggt gtgcagcgcg ggcaacgaca cccccaccgg
     2521 gtactacgcc acgccggtca gttacagctg gcggctgctg atgggcccga gctacggcca
     2581 gtacgccacc cgcatttacg acggctacct gttccacagc gtgccgtatt acagccagca
     2641 caaggacgac gtggagtacg acgagttcaa caagctgggc accgtcgcca gcctgggctg
     2701 catccggctg gccgtggtgg acgtgaagtg gatctatgac aactgcccgc tgggcacgcc
     2761 ggtcgtgatt tataacgaca aggaccccgg cccgatgggc aagcccggca cgatctacac
     2821 cgaccccgac gacgaggaca agcgcggctg ggaccccacc gatcccgacc cggtcaaccc
     2881 ctgggacgat agcttcgaga gcggcacggc catccgcagc caggccgcct gggatcagtg
     2941 ggagaacgag cacgagaatt ggatgtccag tttgacgccc accgatttgc aaggctggag
     3001 cacggattcc aagattgagg gaacgagagg gtaaaaaaga gaagccgcac cgtaggggcc
     3061 ggacatgtcc ggccctcaac ataaaatcag acggtcgtct gcgggaaggc tgcgggccgg
     3121 gcatgcccgg cccctacaag ccgacccaca aaaaagcacc tgctttctca cgaaggcagg
     3181 tgcttttttg tggtactttt agctgaacat caccacatcc tgggcgggct tctcgcccgg
     3241 ctccacagtg actgcggtca ggaccgggcc ggtagaatcc ggggtctggc ctttgtagat
     3301 gggatgatac ttgacgttga ccttagcggt aatgttgatc caactgcgct gttcgagctt
     3361 tttgtagtcg tcgtacttgc agggcatacc cacaaactgg atatcctgca cacagcaggt
     3421 catggcgaag cggccgggca cgaacgcgcc cttgccggcc tgcggggtct ggcagacctg
     3481 ggccagatat tttaccgtct tgccgtcata tttcttcggg tcgtccatgc agtccatgta
     3541 ccagacgccg aagaactcct cggggatttc gatgaccggg gcgtcaatat caaagggcag
     3601 cgggtcgggg atgtcgtcgt aggcaacgct gccgtccttg aactcgtagg cgatatcgca
     3661 gcgtcggctg gcctggcgta ccagtttgtg gataagctgg cggctctcgt cgtcgttgac
     3721 ggcctcggca cggttgacga ccaacagttc actgccgcga atcttatcca acagcaggct
     3781 gcgcatggcg ctgtcgcccg cgtaggtctt gatggtcgtg ccgtcggcag tcgcaaggca
     3841 ctggtagacg agccagttgt ccggcatggc gtcgtacaat tcctgcacga gccacatacc
     3901 attgtactcg atgatgacgc ggccgcagcc ggatttcttt tccagggcgg tcaggttctc
     3961 ctgggtcagc tcgctcttgt cctcgatctg ggcaacgtgt acgccgccga aagcgaactt
     4021 ggaggggtcg tactcgacct caccttcctc gcagaccagc agcagggtct tgtcgccgga
     4081 gtcgaagttg gggtcctcca tcgtttcctg gatgaatttc gtcttgccgc tttccagctg
     4141 gccgacaaag aggtagacag ggatttgttt aggcataggg gtgtcctttc gggagaaata
     4201 ttcgtagggg agggatttat ccctcccgag ccgtgcgcca caactgcggc gctccatcgc
     4261 agccgagttg taggggccgc atatatgcgg cccgcgcgtg tacggcagac ggtcgtttgc
     4321 ggcaaagctg cgggcggcat atatgccgcc cctacccggt ggtataaggc aaaggggcat
     4381 tatccgccgg gcctgcggga gggatgaatc cctccccctg caacatcaaa cgtggaacaa
     4441 ctccgcgatc tcgtgctctt tcagctcgga gccgatgacg cagagcttgc cagtgacgtc
     4501 ggggccgcag gtgcggatct catgctcgcc ggggaccatg tcgaactcgt accaggtgtc
     4561 gccgccgtcc acgatgccct tggcgcgcag gatcatgccg taagcgccct cgtccagggc
     4621 gtgcagggcc ttgtccaggt cctcacggct gtacttgtgg gccgtctcct tgccccagct
     4681 cgtgaacacc tcgtcggcgt cgtggtcatg gtggtggtga tgatgcccgc aggagcagac
     4741 accgtgctcg tcgtagtggt gctcatgatg atgctcgtgc tcatgctcgt cctcatcgtc
     4801 gtcatggtgg tgatggcagc actcgtggtc atgctcatcc tcatcatggt cgtggtggca
     4861 gcactcgtgg tcatgctcat catcgtcgtc atcatcgtca tgatggtgat ggtggcagca
     4921 ctcgtgctcg tcgtggtcat cctcatcatg gtgatggtgg tggtgatgat gctcgtgttc
     4981 gtcctcgtcc gcgtgggcca cgttggcagc gcgagcctcg gtcagcagct cggcgaccag
     5041 gtcgcgcttg ccgtccatga cggagacgat ctgtgcaccg gtcaaggcgg tccagtcggt
     5101 ggtcacgatg gtggcggtgg ggttcttctc ggccagcagg gcgacggcgg cagcgatttt
     5161 ctcctcgctg gcgttaccgg tgcggctcag gatgatgcag gacgcatggc tgacctggtc
     5221 gtcgtagaac tcgccaaagt ttttcatata cattttgacc ttgttcacgt cggcgacggt
     5281 gacaaagctg ttcaggatga ccggcaggct ctcggccacg ccctccaccg cgcgggtaac
     5341 gtcggacaat ttaccaacac ccgacggctc gatgaggatg cggtcggggt ggtaggtctc
     5401 gacgaccttt ttcagcgcct cgcggaagtc gccgaccagg ctgcagcaga tgcagccgga
     5461 gttcagctcg ttgacctgga tgccggcctc acgcatgaag ccgccgtcga tgccgatctc
     5521 gccgaactcg ttctcgatca ggacgatttt ctcgcccacg aacgcctctt tgatgagctt
     5581 tttgataagg gtagtcttgc cggcgccgag gaagccggag aaaatgtcga ttttggtttt
     5641 gttttcagcc atagttgggt acctctattc aagatgtgtt caaatgttca gatagttggc
     5701 aatctattac actaactgct aatactatta taacaaacgc gtcaaggggt tttgcgcctt
     5761 tttgcgccgt aatttttgaa ctttttgcaa aatttattgg attactgcct ctccgtaggg
     5821 gccggacagt ccggcccgcg gcacaccgga aactgcgcgc ttatgggaag gctgagggcc
     5881 gggcatgccc ggcccctaca actctataaa aattcctatt tattatttcc ccaaaaagtg
     5941 gtatgatata cctaacccac atttatatac aaaaaggagc gatccatttt gactaccaat
     6001 gaaaaaatcg ccgcgctgcg tgctgctgcc aaggccgcag gcgcaaacgg cgtgctcatc
     6061 atgaccagcg atccccattg cagtgagtat ctgcccggct actacaacgc actgccgtgg
     6121 ttttccggct ttatcggcga gaactcgacc cttgttgtga ccctgagcgg cagcgccctg
     6181 tggtgtgatg gccgcttcta cgtccaggcc gacaagcagc tggcaggcag tgagattgaa
     6241 tgtatgcacg ccggttccgc cggggtgccc accgtggcgg aatacctcga gaaccacttt
     6301 ggcgcgggtg acaccctgct gctggacggc agctgcgtac ccgccacgat tgccaaagag
     6361 tacatcgacg ccttggccaa gaagggtgcc tccctcaaga gccaggacgt ggcaagcccg
     6421 atctgggacg ccaccggcga gcgcccggcc ctgcccgaca ccccctgcga gctgctgacc
     6481 cccgcccaga ccggggccac cgccgcggac cgcatcgcca tggtccgcgc cgagctgcaa
     6541 aaggccgggg ccaccgccct ggccgtcacc ggcctggact gtgtgggctg gctgctgaac
     6601 ctgcgtgccc gtgacctgcc ctgcaccccg ctggccgtcg cctacgcgct ggtcacgatg
     6661 gacgcctgca cgctgtttat cgcgcctggc cgcctgtctg acgccgacgc cgccacgctg
     6721 gccgagagcg gcgtcgcgct gcgcggctac aatgaactga tcgacgccgt ccacgccctg
     6781 cccgccgagg aagtgttcct ggtggacgag aaggccacca actacgccct gtacgaggcc
     6841 ttgaccgccc acaagaccgt ggtcggtgcc gacccgatct ttgccctgaa gggcatcaag
     6901 aacgaggtcg agctgaagaa catccgcgag tgccacatcc gcgacggcat tgcggtcgtg
     6961 cggttccaga tggacctgga aaaagccctg gccgagggca agaccctgac cgagatcgac
     7021 attgacacga tgctgcaaaa gcgccgtgcc gagatgcccg gctactttga ggacagcttc
     7081 tccaccattg cagcctacgg taccaacgcc gccatgatgc actaccacgc cgagggcgac
     7141 gtgaacagcg tcattgagcc gcgcggtttc ctgctcgttg ataacggcgg ccagtatgac
     7201 tgcggcacca ccgacatcac ccgcacctac cccgtcggcc cgctgaccga caacgagcgc
     7261 cgctactaca cctgggtgct gcagagccac atcgacatgg cccgcgcggt attcctggac
     7321 tactgcaccg gctttgcgct ggacaccttc gcccgcggcc cggtctgggc gcacaaggtc
     7381 aactaccgct gcggcaccgg ccacggcgtc ggatttatct ccggcgtaca cgaaggcccc
     7441 cagagcctgc gcccgaacaa ccccgtcatc ttcaagcccg gcatgaccat cactgacgag
     7501 ccgggcatct acgagactga cgaggtcggc atccgcattg agaacgagct ggagtgcgtg
     7561 gacctgggcg acaaccagta cggccactgg ctgggctttg cgccgttgac actggtgccc
     7621 atcgccaccg agcctgtgct tgtggacgag ctgagccgcg accagatcaa ctggctgaac
     7681 gactaccacg cccacgtcta cgagatgctc agcccccgcc tgcacgagga cgagaaggtc
     7741 tggctgaagg agaagtgcgc accgatcggc cgttgatttt cgggttgcgc ttgacaaact
     7801 gcccgcttac gtgtattata gtagtaattc taaaatgtat aaaggggtga ctgttttatg
     7861 aagcacatcc agactctcga gacccgcgac atggctaaga gcctgctgaa cggtggctgc
     7921 ggcgagtgcc agacctcctg ccagagcgcc tgcaagacca gctgcggcat tgccaaccag
     7981 ccttgcgaga agaccgacaa gtaatttttg ctggctttga tgccggggtg ccgcatggca
     8041 gcccggcatt ttctatgata caaagggtgc ggcgggcgca tatagaatgc gcccctacaa
     8101 ggggaatgcc gtaggggcgg attccatatc cgcccgtggc cgtccgccgc attgcaccat
     8161 aacaaaacgg agaaaaccat gatccatcaa tacaaattaa gcggctacaa tatcgtgctg
     8221 gatgtgtaca gcggcagcgt acacgcggtg gacgatgtag cctatgacgc catcgccctg
     8281 atcgaccagg gcagcagccg ggagcaggcc gccgccgccc ttgccaaaaa atacgcaggc
     8341 cgcgccgacg tgaccgagag tgacatcaac gacgtgctgg acgatatcga cgagctgacc
     8401 gccgcaggcc agctgtttgc gcccgacgcc tatgctgacc acgcctttga cttcaaaaac
     8461 cgctccaacg tcgtcaaggc gctctgcctg cacgttgcgc acacctgcaa cctgaattgc
     8521 agctactgct ttgccgcgca gggcaagttc cacggcgagg ccggcctgat gagctttgag
     8581 accggcaagc gcgcgctgga cttcctcgtc gagcattccg gcacccgccg caacctcgag
     8641 gttgacttct tcggcggtga gccgctgatg aacttcgagg tctgcaaaca gctggtcgcc
     8701 tacgcccgca gcatcgaaaa agagcacaac aagaacttcc gcttcacgat gaccaccaac
     8761 ggcatcggca tcaccgatga agtcatcgac tggtgcaata aagaatgtca caacgttgtt
     8821 ttgtcgctag acggccgcaa ggaagtcaac gaccgtttcc gcgtggacct ggcgggcaac
     8881 ggcagctatg accgcatcgt gcccaagttc cagaagctgg tcaaggcacg cggcggccag
     8941 ggctactata tgcgcggcac ctttacgcat cacaacgtgg atttcacgaa agacctattc
     9001 cacatggctg acgatctcgg ctttacggag ctgtccatgg agccggtcgt ggccccgccg
     9061 gattcccccg aggccctgac cgaagaggat ctgcccaagc tgtttgacca gtacgagctg
     9121 ctggccaacg atatgctgcg ccgccagaaa gcgggcaagc ccatcacgtt ctaccattat
     9181 attctggacc tgaagcacgg gccctgcatt tacaagcgca tttcgggctg cggttccggc
     9241 accgagtaca tggccgtgac cccctggggc gacctgtacc cctgccacca gttcgtcggc
     9301 gacccgaact acaagctggg caacgtctgg gacggcgtga ccaacaccgc cctgcgcgac
     9361 gagttcaagc tgtgcaacgt ctacgcccgc cccgactgca aggactgctg ggcacgcctg
     9421 tactgtgcgg gcggctgcgc ggccaacgcc ctgcacgcca ccggcgacat ccacggcacc
     9481 tacgagtacg gctgcaaggt gttccgcaag cggatggagt gcgccctgat gatgcaggtc
     9541 gcccagcgcc tggaccccga gctggcgaag aacgccgtca agttcgagag cgactgcgac
     9601 ggatgcggcg agggcaacga cggggcgtgt gagaaataac gtaaaaattc aatagtacag
     9661 cgtaggggcg ggactctgct ccacccgggg aaccgtccac agccgcgaac ggacacgggc
     9721 ggagtagagc cccgccccta ctgacattat gaggaaaata ccatgcccta cgattttacc
     9781 accatttacg accgccacgg caaagatgca ctcgctgtgg agggcctggg caaacccggc
     9841 tttgcgccgc ccgcacccaa aaacggcttt tctgctatcc ccatgtgggt ggccgacatg
     9901 aactttgcca ccgtacccac catccccgcc gctatcattg cgcgcgcgtc ccacccgctg
     9961 tacggctatt tcctgacgca ggacgcctac tatgacgcca tcatcggctg gcagcgcgac
    10021 cgcaacggcg tcgaggggct gaccaaggac gccatcggtt acgaaaacgg tgtgctgggc
    10081 ggcgtggcgt cggcgctggg gtgcctggcc gcccccggcg acgcggtgct gctgcacagc
    10141 cccacctaca tcggcttcac gcacacgctg gaggataacg gctaccgcat cgtacacagc
    10201 ccgctggtgc aggacgagaa cggcacctgg cgaatggact acgccgacat ggaccgcaaa
    10261 tgcaaagaaa accacatcca tgcggcgatt ttctgctcgc cccacaaccc ctgcggccgc
    10321 gtgtgggagc ggtgggaaat tgaaaaggcc atggacgtct accgtgaaaa cggcgtgacc
    10381 gtcatctcgg acgagatctg gtccgatatc atcctgggcg gccacaagca catccccacc
    10441 cagtccgtca gtgaggatgc cagccagcgc acgatcgcgc tgtacgcccc cagcaagacc
    10501 ttcaacctgg ccgggctgat cggaagctac catatcatat acaacaaaac gctgcgggac
    10561 cgtgtggccg cccgcagcga aaaaacacat tataacgaga tgaatgtcct gtccatgcac
    10621 gcactgctgg gagcctacac ccccgagggc cgcgcctggg tggacgagct gtgcgccgtg
    10681 ctgaccgaga acgtagacta cgcctgccgg tacattgccg agcatttccc gggtgtgcgc
    10741 gtggccaagc ccgaagggac atacctgctg tttttggact gcaccgactg gtgcgccgcc
    10801 cacggcaaga ctatcgacga ggtggaacag gccggctggg acgtcggcgt ggcctggcag
    10861 gacggccgta tgttccacgg cccctgcgct atccgtgtga atctggccct gccgaaatcg
    10921 ctggtggttg aggcgtttga acggttggat aagtatgtgt ttaacgcata acacgcagct
    10981 ccatttttcc gctgccgccg atatgcttca gccgcgtccc tgccgcctcc aattcacggc
    11041 aggccgccaa aaattccggc gtcacttcct cgccaggggc cagcaagggc acaccgggcg
    11101 ggtaggccca gatgtactcc gctgctgtct cgcctgcggc atcggccagc ggcacggtat
    11161 gggccgggcg cagcagcgct tcggcgatcg tgcaccgcgc cgcgcccggg ggcggcaaaa
    11221 tgtgtgccgc cggggcaggc ggcacggcct gttcctccca ggcgtgcagc acagcggcca
    11281 gccgatccag ggcgttttcc tcgtcgcagg ggctggtcat tgccagtaca ttggggccgc
    11341 agaccatctc cggttcaaag ccgtcagccc gcaggtatgc cgcgccctct gtgccgattt
    11401 gcaataaaat tttaccgctg tcatgtgcaa aaaagcatgg atgtgcgtcc aacgcgtccc
    11461 tgccgaagca catgactttt gttttttgca ggtcttttac agcggcatca aagcggtcca
    11521 gccgggcacg ccaggccgca aaggccgcgc tgccctcctc ggccagccac cgcgtgcagc
    11581 cgtctaaact cgccatcagc gggtagctgg gcgagcttgt ctcgaacacg tccagctggc
    11641 gctccacctc ggcggggtcg gccaaatcgc cgttcagctg caaaaacgcg gtctgggtca
    11701 ggctgggcag cgtcttgtgg gcgctctgca cgaccaggtc agcccccgcc gccaccgcgc
    11761 cgccctgcca gccgtactgc gcggcaaagg gcagatagtg tgcaccgtgg gcctcgtcca
    11821 ccagcagcgt tacgccgcgg gcgtggcaga tttccgcgat ggatcgcaca tccgagagca
    11881 cgccctcgta ggtcgggctg gtcaggatga cacattttgc gcccggctcc gcgtccagcg
    11941 cggcggccac atcggcgggg cgcacgctgc cgtagacgcc gaactgcggg tctgccggcg
    12001 gcgtcagata atggaccgtc agatggccca gctcgatggc gtggtagacg gccttgtggc
    12061 agttgcgggc cgcgatgacc gtgctgccaa agggtgccag cgcgcgaatg cccgccagca
    12121 ggccgacggt ggagccgccc accagatacc agcaccgccg cgcgccgtac agcgccgccg
    12181 tgcgggccat ggcgtcagcc agaatgccgt cagcgtcgtg caggtcgtcc gcgccgtcga
    12241 tctcggtcag gtcccaggca tagcagtcca accccggcgc aggccgcacc cgccgcttat
    12301 gccccggcat atgcagcgga tagcgggcaa cggagagggc ttgtaattgt tgttggagcg
    12361 tttgattttt catatagttt ccttagtaaa acaaaagcct tctccctttg ggagaaggtg
    12421 gctgcgcgaa gcaaggccag atgaggggcg ggtgtgccct gccagcccgg taaaacacag
    12481 ccgcggcagt gcgcccctca tccgtccgct tcgcggacag cttcccccga gggggaagcc
    12541 tttagctcag ttcaatctcc gccttgctgc ccgcccgggt cttggtcaca tggatctggc
    12601 gggtcaggcg gtcttgcagg gcttcgacgt gggagatgac gccgatgagc ttatcgcccc
    12661 cggccaaacc ggccaacgta tccacggcct tttccaagct gtcagagtcc agcgagccga
    12721 agccctcgtc gatgaacagc gtctcgatga caacgccgcc tgcgttctgc tggatcgtgt
    12781 cgctgatacc taatgccaag ctcagcgcgg ccataaagct ctcgccgccg gacaggctcc
    12841 ccaccgggcg ggtcttgccg gtgtaggcgt cgaacacatc cagatccagc gcggtcttgc
    12901 cgcccacggc ctcgctcttg cggcgcagca gacggtactg accgtcggtc atgcgggtaa
    12961 agcgcaggtt tgcagcctcg accacgccgt caaaatagaa cgcctggacg tactgttcaa
    13021 agggcagttt gattttaccg gccaagtttc cgttgatggt tttggacagg ttatcccaca
    13081 tggcacgctt ttcgcgggcg acggcgctct ccttcatcgc ctttttcagc gcgtcgatgg
    13141 cggcgcggtt cgtgtgcagg cggtgtacgg cggcgtcccg ggcctccatg gcggcggcgc
    13201 ggtcgcggcg cagccgggcc agcgcgtcct gcagctgggt cagcgcatcg gggttgtccc
    13261 gcttgggcat cgtctcgcgc atctgctgca cccgggcggt ggcggcagcg tggcccgcct
    13321 tggcgttggc ggcgcggcgg ttggccgcaa agctctgttt ttccagatca gccagctggc
    13381 ggttcaaaat ttccgcctgc ttcgccagat ttttggcgcg gtcggtcttt tgcttcagct
    13441 tcaggtggtc ggcctgcagg ccgcgcagtc catcggccaa gctcttttgc tgggccgtca
    13501 gggcgtcgcg cagctcgtcg gcggtcagtt cggcggcggg cttgccggtg tagcggtctc
    13561 cgcggcgagc gaaaaatcca tctacatccc gctgcaaagc ggtctgcagt tcgttggcgt
    13621 gggccgcggc gtcaccggcc gtgcggctgg cggcggcagt gctgcggcgc tgggccgtca
    13681 gttctttctc gcgctcctcc agctgttttt cggtgatatg atccttcggc agcgccgcga
    13741 ttttggggtg ccgggtcgag ccgcagaccg ggcagggctt gccgtttttc aagtccttgg
    13801 ccagcaggcc cgcgcggttt gcgtttactt ggcgctgcat cgtgctgtat tcttcttcag
    13861 cggcatccag cgcagcctgg gcggccacgt actccgcctg gcgggcagcg gcgtcatgct
    13921 gggctttctc ggcgtcggcc tcgttttgca gcagccgctt acagtcggcg gaaagtcctt
    13981 ccgccagctc aatgcggtgg tcggtcttga gcaggtcagc ctcggcctgg ctcaaggccg
    14041 cgcggtcggc ctcgtttttg gcgatgttct ctttcaaact tgccgtgcgc ttctccaaat
    14101 cggtcatgac caggtcagtc tggtgctcca catcggcggc gcgttgttct tcggccacca
    14161 gccccgccag ctggcggcgg cgggcggcgc gttcctcggc gattttcagc ccggcatcgc
    14221 cccgggcgat cttctcatcc agatccttca tgacgttggt ctggcgtttt tcgttggcgt
    14281 cgtcggcttc cagcaaaatt tttcccagtt cagcggcggc accggcggtg aaggggtccc
    14341 gctcggcctg catttcccgc agatcctcgg cggtctcctc gtcggcgcca tcgcccagca
    14401 gtgtgcccat gtgcatcaaa agtacatcgt ccagccgctt gcattcctcg tccgccttgc
    14461 gggcgtggtc cacagcggcc tggcccacac gctgatgatc cgccgtatca aaaatgcggc
    14521 gcaggatggc cgcacgctcg gcggacggcg cgttgagcag cttggtaaag tcgttctgtg
    14581 ccagcatcga cacctgggca aactgtttgg cgtcgatgcc cagcagcttg acgacggcgt
    14641 cggtcacagc ggatgcgccg ctgaccggct cgtccggctc gcggatgagc tcggccttgg
    14701 cgggcacctt gaccatgccg ctgccgcgct tggctgtgcg ctgctggtcg ggccagcgac
    14761 gcaccgtgta agtacggccc cggtgggtga aggtaagctc cacagaggtc tctgcatcgg
    14821 gcgcagcgaa atcactgcgc agcatcgttg tttttctgtt ttcgcccgat gtttcgccgt
    14881 acagggcaaa cgtaatggca tcgaacaggg cggtcttgcc cgcgccggtg tcgccgccga
    14941 tcagaaacag tccgttgccg ccgaacgccg agaaatccag ctcggtgcgg cccgcgtagg
    15001 ggccgaaggc ggtcaagatc aacttctggg gtttcattcg atttttgacg cctcctcgcg
    15061 cagttttttc acagccagca cttcctcggt cgtcagcggg cgaccgttca tctttgtgaa
    15121 aaagtcctgg aacagctcgg caaagggctt gccgcgcacg gcctgggtca tatcggcggg
    15181 cagcgcggcc gcgccctggg gctggtagtc cagccgcatt gcgttggggt aggcggcgcg
    15241 cagctgggcc atagcgtccg gcagcggcgt cgggtcggtc agggtcgccc agatgtaatc
    15301 ctcggtgtcg gtggggtgct caacgagctt tgccagcggg ccggttaggt ggcgcatggc
    15361 gcggcgcggc atcaggggca gcggctcgat cttttccacg ccgcgcttgc cgatgcgcac
    15421 gagggtgacg gatttctgag ccccgcactc gtccaggtgg tagcacaacg gcgagcccgc
    15481 gtaccgcacg gcatcgatgc cgacgcgctg ggcgcggtgg atgtggccga gcgccgcata
    15541 gcagaagccc gcaaaccgcg ctgcatcgat agaatccacc gtgccgacgg tcaggggcgc
    15601 ggtctcgctg ccggagagct gcggcgggca cacgccggag atgaccatct ggtgcgctac
    15661 cagcacccgg cgcggggcag caggctcgca gacggccagc gctgcgccga cggcagagtc
    15721 gtagtcggtg atatcggcat cgggcaggta gtggcgcacc gtggcggccc gcacaaaggg
    15781 cagcagtgta aattcaatgg caccgtggcg gtcgtgcagc acgacctggc gcggcgcacc
    15841 ggtgaaacgc ccggcgatgt agacgccctg ttcggccagc aggccgctgg cgaagtcaag
    15901 gcgctcggcg ctgtcgtggt tgccggagac cgccagcacc ggaattttgc gggtggcgag
    15961 ctgggtcaag aaccagtcca gcagcgtgca ggcggcggcg ggcggcaccg gcgtgtcgta
    16021 gatatcgccc gccaggacca cagcctcggc gccgtgttta tcgcagaggg tcagaatctg
    16081 gtccaaaatg aaccgctgat cctccagcat atcaaacccg ttgacccgct tgccaaggtg
    16141 caggtcagcc aagtgtagga atttcatgat atatctccaa attttgcatt tgccgtgcgg
    16201 ctttgcggat attgggtgcc tgccggaatg ttcggggccg ggcgtacccg gcccctaccc
    16261 ggcagcctgc cgtttgcagg ggagcggcgt ctccgacgcc ccggcgcttt accgccacag
    16321 cttttcccag gtggtgatat ccagcgtggt gccgggcaaa acctccatgg gggcggtctc
    16381 ggccttgcgg aagcggcact tttttgcaaa gccctcggcc ttcaggtcgc gggcccagac
    16441 tttcatatac aggcggctgc ggccgtcgcc ctcggccagc ttttgggcgt aagccacgat
    16501 gctgcggcca atgccctggc cgcgcgcctt ttccttcaag aacaggcggc ttaaacacat
    16561 tgcgcccgcg ggccccaggt ccagcgcaaa atagcccacc ggctcggtgc cgaggtagat
    16621 cagaaaatag ttgatgacac cgctgtggat ctcgtcatcg gtcaggatgt cgctctggta
    16681 tttatcggcc agtgcggcgg cgaccttcgg cgtcagcttg gtatagtgct ggacgaaaat
    16741 ctcctgcgcc agctcggcca ccagctccac atagcgggtc ttggtcactt gcttgaatgt
    16801 tgggttcatg gcaactcctt ttatcttact ctatcgtcaa acttgccttt gccggcatac
    16861 acgcggccca atcgccctgg tttttcaaaa cgccgaagcc ctcgcaagtg tggtcggggc
    16921 aggggctgtt cacaaaggcg atgccgccgt tttccacgtg caggtgaatg gtgtacaggc
    16981 cggtgtcaat gtcataatcg gcgttcttcc gtaaatctat gcgctgttcg acctgtgggt
    17041 ctccatagcg cagcacggcc gttttgccgc tgccgcccga gcgggtggca agccacagca
    17101 ctgccgcagc ggccagaacg accgctgcga aaatcagatt ctttttccac tttgtcatgt
    17161 tttgtcaaat tccagttctt ccgtcaggcc gtcgtacccg gccacggcgg cggggcaggc
    17221 ggcctgggcg gcggccaggc cgggggcggt gtcgctgaca gcggcgctgt aatgggtcag
    17281 ccacagcctg cgcacctggg cggcagcggc ggtctcgccg gtctcgcggc aggtagcgtg
    17341 accatagagc ttcgccttgt cgaggtcggc atcgtcggcg taggtcgcgt ccatgcagag
    17401 caggtcggcg tcccgggctg cctccacaat ggccgggcag gggcggctgt cggtggcgta
    17461 gactactttc agcccgcgcc ggggtgcgcc caacacctgt tcgggcgtga agccgccgac
    17521 gttctccccc ttttgcaggg tgccccagta ttcaatcggg atgcccgccg ccctggcgcg
    17581 ggcggggtca aacttgcccg cgcggggcag cgccagcgcg taaccacagc agggcacgcg
    17641 gtgggtcagc gggaacggcg tgatttgcag ctcattcacg gtgaacggcg cgtcggcctg
    17701 ctgccagcgc accgggtagg cctgcggccc ggccagcgcc atgatggctc ggctcacagc
    17761 ctcgatgccc cgggggccgt agatggtcag cggctcggtg cggttcagac tgcccagcgt
    17821 ttgcaaaagc ccgggcagcc ccagaatgtg gtcgccgtgg tagtgggtga gcagcaccgc
    17881 cgaaagccga tacgcactaa cgccgtaccg ccgcaggccg acctgcgtgc cctcgccgca
    17941 gtcgagcaaa atgttatgcc cgcccaccga cactgccgcc gcggccagcg cccgttccgg
    18001 caatggctgc gtgccgccgg tgcccagcag ggtaactttc agcatagatg cacctctttc
    18061 caacattatc ccattattaa acgtattata gcatagattt cagtgtttcg caaatttttg
    18121 aaacacccca cccgattttg tcgtataatc ttgatgaaag ggggcggcgc ggatgacgga
    18181 gcgcgagttg gacatactcc tgcgggagta cagcggcgcc gtgcgggcgg tctgccgggc
    18241 aattttgccc ggccacccgc aggacgccga ggaggccgag gccgacacat tttacaagct
    18301 gtggcgcggc gcgcggctgc ctgcagacga ggcgcatcgg cggcggctgg tcgttttgac
    18361 ggcgcggcaa tgcgccattg accgctaccg cgccctgctg cgccggggcg agacgctgcc
    18421 gctggacgac cgcgatgacg ccgagctggc tgtggcgctg gaaagccatc ttgaaagccg
    18481 cgagctgatg gcgcagatca tggcgctgcc gccgccggac ggagagctgt ttctgcggcg
    18541 gtacttatac ggcgagacgg cggcccagct gggtgcgcgg tttgccatgc ccgccgccac
    18601 ggtgcgcacc cggctgcacc gcgcgcggct ggcgctgcaa aagctattga aggagggctg
    18661 acctatgacc gaactggaac gctggatgaa cgaccacccg ccgctggcgg acgccctgac
    18721 ggcggaggaa agcgcagcac tgcgccaaaa agtactggcc cgccgcccga agccccgggt
    18781 tttgcgcggc tggcggctgg cagccgcggc ggcagcgctc tgcttagtgg ccgcgggcgc
    18841 ggtggtgctg gggcttttcc accccatgag cgccgccaac gagacgaacg cgctggtgca
    18901 gaagtacggc gaggtgctgg acgaaccgct gaccgccgag atcaacggcc gcacggtcag
    18961 tgtaaaggcg ctgctgcgcg gcgaaaacac gctgcgagtg ctgtacgatg tggacaacgc
    19021 ccaagggatg gagccaaacg atttggtgtg ggacaccggc gcgttttacc tgcgtgcagc
    19081 ggacaacagc ggcgacaagc ggctctatat tgcaagcaac gcacaggtag ggccatgtta
    19141 tgggtacgcc attggttcgt gggcaacgcg caacgcggtc agcgaaggcg gcgttctctc
    19201 ctgctatgtg gatattccgt tgacccagac ggccaccgac acgcgaacgc tgtatatctg
    19261 cgatttgaca aatcacaagg cctctgcaca tgttaaggtc atgctgccaa acatggctga
    19321 agcagtgacg ctaaacgtaa agaaaaccat cgcgctgaag ggcgggcact ggttccatta
    19381 tgatatgacc gaggaagaag ccgagcaata tcccgacgca acgtttgaca ttcaagaggt
    19441 tcgtattgaa ccgctggcgg tcagcgtcat cggcaagaat cacggcagtc tgccgctggg
    19501 cgggacctcg cggtacgatg tgcagtcccg cgtgaagctg ttccgcgctg acggcacgga
    19561 actgacctac ggctgggacg gagagtttta tatggggcag tatggccgca tcaccaaaaa
    19621 ttccgggaag ataaccctgc gtgtggacag ctacgacatc atcgacccgg aagcggtggc
    19681 gtatgtcgag atcgacggag agcggtttga tgtgaaccgg taagccttcc ccccgagggg
    19741 gaagccccaa aaaaagcacc cgcatggcgc gaaccatgcg ggtgcttgct ttttgcagaa
    19801 ttatccgaat cagccgaaca tattgatcag gataccagcg gcaacggcgg agccgataac
    19861 accggcaacg ttggggccca tggcgtgcat gagcaggtag ttgcgggggt tgtactcctg
    19921 gccgaccttc tgggaaacac gggcggccat gggaacggcg gaaacaccgg cggagccgat
    19981 gagcgggttg atcttgccgt gggtgacaac gcacatgacc ttgcccagga taacaccgcc
    20041 ggcggtgccg acgccgaagg cgatgatgcc cagaacgatg atctcaacgg tctggatgtt
    20101 caggaaggtg gcggcgttgg tggtgcagcc gaccgagaag ccgaggaaga tcgtgatgat
    20161 gttcatcagc tcattgccgg cggtcttggt gatacgctca acaacgccgg actccttcat
    20221 caggttgccc atcatcaggc agccgaccag aatggcggcg tcaggcaggg tcagggcaac
    20281 gatgatggta acgatgatgg ggaacaggat cttctcggtc ttggagacgc ggcggggggc
    20341 agtcatgacg atctgacgct ctttttcagt ggtcaggacc ttcatgatgg gcggctggat
    20401 aaccggcacc agcgccatgt agcagtacgc agcaaccgcg atggagccga gcagatgcgg
    20461 ggccagcttg gtggtgacat agatggcggt ggggccgtcc gcgccgccga tgatgccgat
    20521 agcaccggct tcctgattgg tgaagcccag gatcttagcg ccgacaaagg tgaagaagat
    20581 acccagctgt gcggccgcgc ccagcagcag gctggacggg cgggcgatca gcggctcgaa
    20641 atcggtcatc gtgccgatgc cgaggaagat caacggcggg taaatgccca gcttgacgcc
    20701 taagtagata tagtcaacca taccgccgga ggaaccgagc agcgcccagt cgatatgatt
    20761 cgtctcgttg atgaagatgt ccatgtggaa gatgccgtca aggggcaggt tggtcatcag
    20821 cataccgaag ccgatgggca gcagcagcag cggctcaaac tgcttgacga tggccagata
    20881 cagcagcacg caggcaaccg ccagcatgac ggcgtacttc cagccgccct cggtgaagat
    20941 gatctgcgcc cagccggact tggcgaaaat ctccgcgatg ttcttaaaaa tttctcccat
    21001 ttccgtacct ccttacataa aaggcgtggt gtgctcgcgc acaccggcct cgccccacgc
    21061 accacggcgg gagccggcag cgggcttgga aacgcgcttg atgccgcgga tcacggcatt
    21121 gccgccgcac acagaggcca cggcagcgga gatggcggcg atgatctcgc cggggatgga
    21181 gccgttgtca gccttggctg caggggcagc gggcttggcg gcaggagcag ccttggcagc
    21241 gggggccgca ggggccttcg gcgcaggctt gccgttcttc ttattgttga tgttgtcgaa
    21301 gatcttgcct tccagcgtga tgataaggca cagggccacc agcatcgcga aaaccagcgt
    21361 aatgctggtc agaacaacgg tgccgtcgct ggcttccagg gcaagcagag ctaggttggg
    21421 catggatgat cctcctttaa ttggtaaacg gccggaaaaa ggctatttga tgatattata
    21481 gcacaaagag aatttttttg gtagattttt ttacaaaaaa atgaataaat ttagggaaat
    21541 gtacagatgg tgcatgtagg gggcggcaga tttcggcggg gtgagggcac cccgccctac
    21601 gcacgggttt gatgtggggt tgtagggtgg ccagccctct ggccgccgtg gccgtctgcg
    21661 gcggcgggaa ggtgtgcggg agggatgaat cccttccccg acggattgaa tcgtaaaaat
    21721 gatatggcgg taagctgcgg gccgggtatg cccggcccct acatgatgca caattttgca
    21781 agtggatttt gggctgatta cacagcactt tttacaaata tgaacaaaac atgccttttc
    21841 cctcttgacc aatatgcaac tctgtgatag cataagcgca acgaaagaac ggcaaaggcg
    21901 ccgggcagag tatttctgcc cggcgctttt tctgtttctt ttgcaagtat tctgtgcgaa
    21961 agtaagagag aggggtttca aggtatgttc aactcagcag atgtaacctg gacactggtt
    22021 gcggcgttcc tggtgttctt tatgcaggca ggctttgctt tgtgtgaagc aggcctgacc
    22081 cgcgctaaaa acaccggcaa tatcctgatg aaaaacatga tggatttttg catcggtaca
    22141 ccctgttact ggatcgtcgg tttcggcatc atgttcgccg gttccggcgc actcatcggc
    22201 gggtttgacc cgttcatccg cggcagctat gacttcggca cgctgccggt ctgggtctac
    22261 gcggtgttcc agaccgtgtt ctgcgccacc gccgccacca tcgtttccgg ctcgatggcc
    22321 gagcgcacga agttcagcgc ctactgctgc tactccgccg ccatcagcct gatcgtctac
    22381 cccatctccg gccactggat ctggggcggc ggctggctgg cgcagctcgg cttccatgac
    22441 ttcgccggtt ccacggcggt ccactttgtc ggcggcgtca cggcctgcct cggcgcctgg
    22501 atgctcggcc cccgcattgg caagtacaac aaggcgggca ccccgcgcgc catccccggc
    22561 cacaacctga ccgccatggc gctgggcgtt ttcatcctgt ggttctgctg gttcggcttc
    22621 aacggcggct ccaccgtcag catgaccggc gatgacacga tgatttctgc tggccttatc
    22681 tgcttcaaca ccaacctggc cgctgcgctg gccactgttg cggcgctgat cgtctcgtgg
    22741 gtgcggtacg gcaagcccga tgtgtccctg accttcaacg gcgcgctggc cggtctggtt
    22801 gccattactg cgggctgtga tgtggtcgat ccgttcggcg cagccgtcat cggcattgtg
    22861 gccggtgtgc tctgcatctt ctcggtcgag ttcttcgaca agatcgccaa gatcgatgac
    22921 ccggtcggtg cggtctccgt ccactgcgtc aacggctgct ggggcacgct ggctgtcggc
    22981 ctgtttgcca ccgagggcgg cctgttctac ggcggcggct ttgccaagct gggcatccag
    23041 ctgctgggtg ttgtcagcgt ggcggcctgg gtgctgatca cgatgtacat catcttctcg
    23101 atcatcaaaa agacgatcgg gctgcgcgtc tccgagaagg aggagctgga cggcctcgac
    23161 atccatgagc acggcctgac cagcgcctac gcaggctttg ccatcagcga cccgacctac
    23221 gccgagctgg acgtgaacga gaacaccgac ctgggcgagg acgacatcac caaggccagc
    23281 cccgagaaga tcgcggcggc cgtcaaggtc acgcaggaag ccctgctgcc cgccgagctg
    23341 gattccggta tgcacaaggt ttccatcatc gtgcagcttg ccaagtttga ggcgctgaag
    23401 aaggcgctga acggcatcgg cgtcaccggc atgaccgtga cccaggtcat gggctgcggc
    23461 ctgcagaagg gcagtggtga gaagtaccgc ggcgccgagg tggacgccac gctgctgccg
    23521 aaggtcaagg tcgaggtcgt tgtgagcaag atcccggtgg acaagatcat cgacaccgcc
    23581 accaagacgc tgtacaccgg ccacatcggc gacggcaaga tctttgtcta caatgtggca
    23641 aaggtcgtca aggtccgcac cggcgagcag gattacgccg cg
//
LOCUS       DBMDSQ010000003        23594 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_745_length_23594_cov_2.104967, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000003 DBMDSQ010000000
VERSION     DBMDSQ010000003.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 23594)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 23594)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23594
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_745_length_23594_cov_2.104967"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..728)
                     /locus_tag="ACZPAX_00210"
     CDS             complement(<1..728)
                     /locus_tag="ACZPAX_00210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1112350.1"
                     /translation="MEIAPMTKRDKILLYVVSMFAFIVLFVRFLLIPGIENHQQAAAD
                     LTEAQDAQIAMQDTILKAEFNANTKNTNWGELQSANALYYSILTSDELDTLVTGLELN
                     HSLQPTSLSIGQPATQNLSWYIASESAGQGAAPSAGTTAAVTADDVGTTAEGNAILQQ
                     FLAADLIPDYDQPGYFKTAAVTFSCTGSDTDFIAMLDDLAAGYPSVQLQTFTISTRSY
                     ANPDGTSEEGSVYAVSLNVILCDKG"
     gene            complement(742..1308)
                     /locus_tag="ACZPAX_00215"
     CDS             complement(742..1308)
                     /locus_tag="ACZPAX_00215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1112351.1"
                     /translation="MAMKQNNFYLRWQKAAGAAKTESRSKDLRAVVPGIAVVAVCMAG
                     WGVMVLHTSMLKSRRQNILNWCADNSATYLSSTLDAEEAAEFRSLTDYATGMTNLLNS
                     YPDVTSTLLHRIEAAGGASITIQFTSYNASTGELLFNANSSQVIDIPTYIRSLQNCGV
                     FSTVSYTGYTADDNGYSIDLRCILAAPQ"
     gene            complement(1344..2237)
                     /gene="pilM"
                     /locus_tag="ACZPAX_00220"
     CDS             complement(1344..2237)
                     /gene="pilM"
                     /locus_tag="ACZPAX_00220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilM"
                     /protein_id="HIR1112352.1"
                     /translation="MATLTSLYLCSRTVYAAVGSPTAGGARITAAAQMQLPEGCLING
                     VITNEADLADALKAFFAQNKLPASRVALIAGGSQFMHRILSLPAMSEKKRMAVLSHEL
                     STGGAETKAPLDDYMLLSRDAKTRTDTVLATRVEQSVIAGYDALAKAAGFKLTCIDLG
                     LAAPIKAVRTIPDLQQKTFVLLQFDDDTISATLFVRGQYSYSTRSRLFNPRGSVESGT
                     EIAQKLSGILQFHIANKSEHQIGTVYFAGSTAEDFAVCRPGCEALGLAVEIFPDSPTV
                     KLPSGTHLADVLYAAGNLIAR"
     gene            complement(2260..3471)
                     /locus_tag="ACZPAX_00225"
     CDS             complement(2260..3471)
                     /locus_tag="ACZPAX_00225"
                     /inference="COORDINATES: protein motif:HMM:NF012694.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HIR1112353.1"
                     /translation="MPRFRYTATNAQGKTVRGVTDAPSEEALYTKLRGDGLYMTDAQE
                     AQKSHSAFRPLKTAMLADFCRNLGTLLAAGVPLVRAIRIMADERNIDARQKALYEAIL
                     GELRKGVPLSDAMESCAPAFPNLLLAMIRSAEGTGSIDHACMRMATYYEREHKMNQQV
                     GNSLMYPIILGVLIVAVIAILMTFVLPQFKPMFDQMETLPFLTQLLFDISDFVGANWP
                     VLIVVVLLLILGGRILLAQPKVRRQWDRFILHAPVVGVLNRKICTARFASTLSNLYGS
                     GVPIITTLGAARDAIGNRWIESQFGDALDSIRAGHSLSQAIAGIDGFETKLSSSIAVG
                     EETGRLDSLLATISETLDYEAEMTTKRLVTLLEPVMIVIMGIVVGGVVAAVIVPIYQS
                     YGTIGSSSGAI"
     gene            complement(3540..4331)
                     /locus_tag="ACZPAX_00230"
     CDS             complement(3540..4331)
                     /locus_tag="ACZPAX_00230"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: protein motif:HMM:NF013632.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HIR1112354.1"
                     /translation="MLLLECLLGLIRFLLGAALFSFMNVVAWRLPRNMDPLKGRSICP
                     RCGRTLDAPDLVPVFSWLFLRGRCRHCGAHIPARYLLVEVLGGVLALGCTRQYGAALA
                     LPDGLFGMSWAALLALAVCGILLSIALIDAETQTIPDRLNFALAVCGVAGTALSPAGW
                     RGHIIGAVCVSVPMFLLCLAIDGAFGGGDIKLMAAAGLFLGWQNTLLAMFFGILGGGV
                     YGMYLLAAKKADKKDHFAFGPFLCVGIVLAMLFGEPVLGWYCQFL"
     gene            complement(4318..4797)
                     /locus_tag="ACZPAX_00235"
     CDS             complement(4318..4797)
                     /locus_tag="ACZPAX_00235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1112355.1"
                     /translation="MLAARLGAWLRKTGERWAKAAFSEKLAVALALAAVIYTVVTGAA
                     ELRYQARAREALAQVKAARLAAGAVSAQYYATGRPYADQTSPDGFADGVADAIETLGA
                     LPGTVTLLQIGGNGYTVEKLLYCENGMYAIYDAAEGYRVFRAEDRLQYSAEVGHAAS"
     gene            5082..15731
                     /locus_tag="ACZPAX_00240"
     CDS             5082..15731
                     /locus_tag="ACZPAX_00240"
                     /inference="COORDINATES: protein motif:HMM:NF019575.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HIR1112356.1"
                     /translation="MLHQPKNKKRTGGFTMVELMVVLAIMGILAALVGGGLIAYTRLA
                     RFEKNEANARTLFQAAQISLTRMDTAGELDAFCADVEDQGKAGTHFTDDVVITDAAGN
                     EVRRRTASELNNGIYALYYDKTDGTGQNELVRRLLDDYIYDASMFNAAICVEIDRASG
                     QVYSVFYDTNSDKLRFAADGVADATDITDRSYDHRRNDSLVGYYSAEDRVNVVELQQT
                     KLKVKNPRLSNGETLTLSWGGNSNLADMDTVYTAVAYRAEKNGLTGYKIADESKPLFS
                     IEIKRPNTEQQVLAELETTVYTYSADGTRTETKRTLRYPLSYNKGNFVLTLDAMADAS
                     LLRACEKDTSTADNDLYSIIRLLAEPENIIIRLQAAPRDEYADSYTASKSEDSNAENT
                     LFAIDGTKTDAELKYFRHLYNVRWAKNWGDAKATYTLKAQGMGANGLNWTGGGVTVYT
                     AAGVGKNPEAKVPSQTDPVAWPTIPALGENITLQSETTVAGTARVPILNLQLRGNSVA
                     AVDKTNENPLDRYVGLVGENNGTISHITLRDADVQVNTEIKALTTGQSVPEGGIRLTN
                     TTYLAELPEGDTARRDGVRAVGALCGVNTGTLQDCTLLHGTNNAYKAQVLATLHFDDD
                     TTEKTRIPDAANKYYEHEPQGIGGLVGVAIPKNDAGTKLENLTVGENVTVAGLLVDDD
                     TTDTTDEPGEAGEKARYTAVAADAGDTKTATRWRSVGVGGVFGTLDAANLKTDDKTVI
                     ANAANVTGSGFVGGIAGNLYSTDTAADGTIKTIVNRLASTGTVSAGVNYQGDNTQTKH
                     SLVLGQFFGGIAGYTQDVVLANCTSTARGTMTETDLRSQVDAGCASDGTLNDNSPLKG
                     DFVGGLVGFGKNILLQNSRTEKGYVLGSRFVGGLAGGFTGSTLHADSAANASDVFGNR
                     YVGGIVSVNGSGSIIQTSTNSGLVAGLGKNAAYVGGIVGVNDATWGAGSAAQAEEVQL
                     VNCTNSMSGDNATDSRRIRLLQALSTHAGITEYANYVGGLVGYNGTKGSVTWDRYGTP
                     TLGAILYGGTFVGGLAGYNDGNAAITNTSGKPLTIRGQIVADGDAVGGLIGLNTAPVL
                     PTATVAATRVEGVHFVGGVIGANMPVGSFTVQDTLGKAGALTTTVTAGRVRADGVAGG
                     IIGYNRLLAALPQDVTHAAMLPTFSDDNVLTDSTAVTSSEAAVCLENFTNTFNLEANA
                     YVGGILGYNDAATNLTIRNAVNGSSSNALAVGGLSMGTDDTAHRLYQGVKLLSLNQNY
                     EFGETRGYLAGGILGFAAKNTTLENCTSYGNIAHACAAGGLTGWNDGTISGGMTCASL
                     GSQQAGYLYLGGIAGVNTRSIANAAPDQGVVLRGNQVIGGIAGINMSGAAVTYDQAAT
                     PAASVVATYCAGGVAGVNCGTITLGSTTQLNFTVTATTYAGGIAGSNNARGSTRGLIT
                     GGVPIAQVTAATYAGGAAGINYADISNVNNQAPVRANDTLAGGIAGQNAAGGVIDGCR
                     DTNREVYATNGVAGGIAGTNAAGAAIRNAEITAGATITAANGAAGGFVSENSGLIENG
                     TVRGGTINFTGGTIGAVATYNRKGGTIRGTAVAQNAKVHLNGPATIVGGIAGRNEPGE
                     GTTDAAWIENAAVNAGALDLNGLTVNANLVTLGGAVGSNAGKVKGTNVTLSVTEGDKL
                     ERYQNLGGVAGQNDGTLDTCTYQGTLGLASAKADGDFAWGAASRDGNTIGGIAGVNNG
                     TVQNCAVPAIVLQARGSNNVSSLQTDAQKLDNATHVGGIAGRNNGTISASYVATDANG
                     KSIIAARYGFVGGVAGSNSGSITNSGSQQAQALVSKVNNEWLPLNTDDANTGINAMVA
                     ELTKTDGTYTGFKGVDTVGAHDYGYGSVYLTENKTTTSGGQWGTTTTTKAGLAANDLT
                     VVLRGNSNVDNNDSQRGSGYLGGITGYNSVTGAITDSATGRWFVYGDNISKASTIGGI
                     VGTNISSKDLTTLVNCAAVRRYTRLSSQKNDDNTTNEHTGAKATRIEVHVGGIIGHQG
                     NDRDDLWALSKCVNYGTVYNSRSNNIGGIVSYWTDYGGTIEYSFNFGDLLTNMNDNWD
                     SCGTMGGIAGYFDAPVKNTSANILHCQNHGSMKGTNNKSANDIGGIFGKVQMADKTDP
                     MTINVYDCVNGSTVEIKAQSMAVGIFGYLGPWDTRGSTIDNVIVNIDRCRNYCTNMNA
                     SYKVGIVGNRGGGGRSTQPTTLNNCFSLVGGDNWHPLAYDSPVTAVENIKGSNNYYID
                     AYSKDGKSAWNAQAWNERWLGHTSTNLTNFTWSGADSRFTESMETKNCGGRRLNLGKD
                     TTDDSYFAMLAVPNNTNDNTTYDLSRVVYNTAYIHVDATDTWKRTVETKDGDVLGNIL
                     LRFTETNDSIKGSSGQNIPSNGDITDEVIQNYYKYVLEANEPNQIPESITIQRSEETK
                     DGTGAVYGRYLVSWTAPKQDAAHGPAMYYKLEVYKTDASGKVTDATPIHTAMVYETKY
                     TFDNDPDRDAWTGNFVVRITPMNSKGGGTSRDSAVQTFAQTLPKPEIEVRLKRTGTGN
                     IYYQTVVLTNYDEYVRIANDQWKVDFNYVDGSSGSSGSFSKGKSEQTITNGLDNIRTL
                     TAIANPSTSTAPFMRSEQFSERTAIAGPFTNPDKYDEWNTGLAAKGNVTVTNPTVIGT
                     TVNDLTISLNLSYAPFVRDDNKMQEAQPIYRVMLVGKYVGDSETTASGTSLTGQYITL
                     AAQQTRVGAAATTVTFRNLPKEAFTSDYEDLKIVAAPVESGIGPSYSRWAATEEEVTE
                     AIAQRGTDPVAYYDGLEIVRKAGGDGYEYAHMTALYFVGKAYNTNDKDKSYNDVLAPS
                     QVKTLSTGVTTLQAPTLDQQLTKEEVRTAENKLYYTFSWTQTTDGTIPDTTVQKYTIE
                     LYGVQADGKQETIRLASGVDLADRVKFDNAGRYTLELCVDDDLAEGSKSWKYNNVILH
                     VARVSDTNAQIGLADEQSYAVMTRLDAPDAPGGFTRLYDENSADALTYSINWPAVVGA
                     DHYTLYAKRQNADTTWETVETWENITGTKTEIDMEQYQGQELRFYVIANRAAGDTTGF
                     DSPDGALCDPQAVKSRVTAPTVSSAVFAPAAPSQAEFLNGETLQMMVSGNTASSYFFT
                     GYLFKNVDDYQTIAALANDWQTTAALGKDKADKLAALQTALNEMLKNGDAICIIPESD
                     RQSGGGTPADGSGNSAAYNLSNGFTMKPEYSRYYLLPALRAMAETSSGMESSNWYYYV
                     GDYMNNPAAMQLPAIKLDAPAAKLDNVTVTHAAQLYDSGSYERPMGDPISLDITHFAV
                     QWKAANRYPSIGELAGGMVRNLTDHYKFTVTAKDGGENYTIEMWTYDADSEEKKDDGT
                     PEHLRGEIKTVKKTVTIDGNNYEKYLDPQIEKDADGNVTRTWYDLSLTPATKDGGSRD
                     TNNWVSQPVSLRGRITQKDSDAQPYYEVADAIATLQVVDDDGEPAYRVTLPDMQDILT
                     TGDKLHSFTDSILVETLVHSSGDGKTVGTPDAEAVKIGDETQTVELIAEEPTPDENTE
                     TTQPTETPAEPQNAPQPVELTDKAG"
     gene            15755..16549
                     /locus_tag="ACZPAX_00245"
     CDS             15755..16549
                     /locus_tag="ACZPAX_00245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1112357.1"
                     /translation="MRRKARDILRDQQGLSMILVLCIGALFVALSAALVYAASVLTAN
                     ANRQLLEQEAYQLATSFSDVLEGELNTPTSSFAAFVNDSFMTSTAYGGDIYGSPSQPY
                     SYTWKAADADTSGAEEITVTLRRRPGEDAEKLNLSGVGQSVSTKSGWRDQLTSWQSND
                     YLLTDLQLDVIVTATKAGESFAYTVTYDRQVHYPVSGYTFDDDTTVYTWSKGTNTFAS
                     PGLSSIDIKPEDAERIIVPHFDTTNPDKSTIHYTRGARQSTSTAQE"
     gene            16556..16948
                     /locus_tag="ACZPAX_00250"
     CDS             16556..16948
                     /locus_tag="ACZPAX_00250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1112358.1"
                     /translation="MKHKLQNQRGETIVEVIVSFMLLLMFTAMFVVSLRYARAMSQKA
                     ETLRETAYAFCAGLYPQEKQTPAWQTEKTGQSFTFSAGAGAGAFTVDGVELDQVETQA
                     EVSENGGTPTTETHTFHRYAKEAGEATP"
     gene            16945..17706
                     /locus_tag="ACZPAX_00255"
     CDS             16945..17706
                     /locus_tag="ACZPAX_00255"
                     /inference="COORDINATES: protein motif:HMM:NF019575.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus modification PilV family protein"
                     /protein_id="HIR1112359.1"
                     /translation="MKQLQNKRGTTLVELIVCMVLLSILTLAAVTLIRPSAQAYRDIQ
                     LQTRAQNLADALIETIRGEVLDANGYIRFTNGATDSANLDSVFDAQTSYSDGTALEFS
                     VYPNHVELIDKDLVPALKNSKGKDLLTQAQAEELNGYLHMRFYQQEQSDFAPLHKKDG
                     EKIAYAYTTAYPKESYMGLYISDLHFYARSWAQENDTDTPRITAMTVVITVAKRDSSG
                     NDVPLCTQKAIVPLPGEPVILYAPGTWNGKASSER"
     gene            17828..19045
                     /locus_tag="ACZPAX_00260"
     CDS             17828..19045
                     /locus_tag="ACZPAX_00260"
                     /inference="COORDINATES: protein motif:HMM:NF015649.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AfsR/SARP family transcriptional regulator"
                     /protein_id="HIR1112360.1"
                     /translation="MATIEIRMLGDFEIRVNGKPVLAVLGQSRKTTALVQYLVLQRGE
                     RVTHKVLTDTLWAGERSSNPDMALRAILHRFRNMIEAEGLDELRNCILTSRGCYQWNP
                     HLDCTVDVFRVGDLSEQARREEDPEKRAKLYEQIVDLYRGRLLPQSASEQWVEGVSVR
                     LHGQYRTAMLSLLELCKKWGESTRIISLCNHALELDPYEERLYLEEIVALEALGRHNE
                     AQKLARRGTAMGCLHHAIEPGRAGSAYRQMRQADRNMEGEVAKLVAALPDTENAGACI
                     CDFETFKEAYRVQRGVQARYGLPVFLAIMTVAPGQDAEPDETSTMVDQLGELIHTSLR
                     QCDVAARYTETQYVIMLCGSAAETGSSPLERIKASFYRVPAHGRYLLSYSLYVPEVKP
                     DGGRARRKRSKKQ"
     gene            19291..21363
                     /locus_tag="ACZPAX_00265"
     CDS             19291..21363
                     /locus_tag="ACZPAX_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007048239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HIR1112361.1"
                     /translation="MGYASKDIRNILVAGHAGCGKTTLTEALLYMSGATERMGRVEDG
                     TTASDFDPEEIRRKASLNSSVVPVEFEGVKYNLIDAPGLFDFETGAAEGIMAAESVLI
                     CVSGRSGVTVGAEKAYQMALKNNKARMIFVTKTDLENADYFKILEQMKIKFGPSVCPC
                     VVPVRLDDGTVAYINLFSQKAFKYEGGKQIQIDVPDIGHRFEGLIQAMSEAIAETDEA
                     LMEKFFEGEAFTTEEIVQGMAAGVRSGQITPVFCGSAVNGQALDMLIYNMNVLLPGAD
                     TASAVGEANGEPVEITADPAAPLCAYVFKTVADPFVGKLSFIKVLSGKLTATSNAINA
                     RTGQPERLGKTLTVCGKKQTDTPEIAAGDIGAVAKLATAKTGDTLCDPARVVALPAPV
                     YPISCYRMAVKVAKKGDEGKVGGALTRLIEEDPSIKFEVDPETKQQIISGLGTQHLEV
                     ALAKLKNKFGVEITLETPRVPYRESIRKSCKAQGRHKKQTGGHGQFGDVWIQFEPVQG
                     EAIEFAENVFGGSVPKNFFPAVEKGVRQAAEHGVLAGYPMVGMKATLLDGSYHPVDSS
                     EMAFIMAAKLAYKAAIPEAGPVLLEPIGTLKAHVPADNTGDIMGEVTKRRGRVLGMNP
                     DDDGLQVVEAEVPMAEMQDFTTFLRQLTQGRGYYTFEFVRYETLPQMLESKVIEKAKA
                     LGNFADED"
     gene            complement(21485..22474)
                     /locus_tag="ACZPAX_00270"
     CDS             complement(21485..22474)
                     /locus_tag="ACZPAX_00270"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR1112362.1"
                     /translation="MKASVIIPNLNGAGWLRDSIESVWAQTEQDFELIVIDNGSKDES
                     LEIARSYRGRDRYTLIENAENTGFSHAVNQGIAIAKGEYAVLFNNDAFAEPDWLAELI
                     RTADADPKIFAVSSLMLRFYEPELADDAGDYVTILGFACKRGDGLKASRYTKPCRIFS
                     ACGGAALYRKSILEEIGVFDELFFAYYEDVDLSWRANSLGYKNVYCPTAKCRHICGAT
                     TGAVRYNPFKSIQSGRNSILLPYKNQPLLMLVINFIPMLLGYLLKVLMFRLRGFGNDY
                     AKGFHEAWVALPKIQKPKFRWRNLPNYFWVEGSLIVGLFRYIVYRVQRALKIT"
     gene            complement(22484..22906)
                     /locus_tag="ACZPAX_00275"
     CDS             complement(22484..22906)
                     /locus_tag="ACZPAX_00275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1112363.1"
                     /translation="MLRYCKNCGLLLPHDADRCPQCGAPAPLAVRPAAQPGPAAPLYG
                     TPDDREAEPMAEAMSQGATTGTMILFAIPVVGFILALVWSFGGTHNEARKRLARAQVV
                     RTLVVAAAAALLVLVAALVFSAVVHSQLAYYYATPYYR"
     gene            complement(22917..23351)
                     /locus_tag="ACZPAX_00280"
     CDS             complement(22917..23351)
                     /locus_tag="ACZPAX_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HIR1112364.1"
                     /translation="MDDLDHKIIQLLAENARMPVKDIAQQVNLTSPAVSSRIHRLEQE
                     GIIGGYTVVLRHPDAPARVEALISVLVDAATRASFLAMIEQESQVLQCFRVTGSYNFI
                     IKVSCTDIDALEHLLTKMQKMGSTNTQIILNTQLDRNLTLAE"
BASE COUNT         5262 a         7188 c         7084 g         4060 t
ORIGIN      
        1 cctttgtcgc agagaatcac attcagggaa acggcataca cagagccttc ctccgacgtg
       61 ccgtcgggat tggcgtagct gcgggtggaa atggtaaagg tctgcagctg gaccgaagga
      121 taccctgcgg ccagatcgtc cagcatggcg atgaaatccg tatcgctgcc ggtgcagctg
      181 aaggtcacgg ctgccgtctt aaagtagccg ggctggtcat agtcagggat gaggtccgcc
      241 gccagaaact gctgcaaaat ggcgttgccc tcggcggtgg tgcccacatc gtccgccgtc
      301 acagcggcgg tggtgccggc actcggcgcg gcaccctggc ccgcgctctc gctggcaatg
      361 taccagctca aattctgggt ggcgggctgg ccgatgctca aggaggtcgg ctgcaagctg
      421 tggttcagtt ccaggcctgt caccagcgtg tccagctcgt cgctggtcag gatgctgtaa
      481 tacagggcat tggcggattg cagctcgccc cagttggtgt ttttggtgtt ggcgttgaac
      541 tcggccttta ggatggtatc ctgcatggcg atctgggcat cctgcgcctc ggtcaaatca
      601 gcagcggcct gctggtggtt ctcgatgcca gggatcagca aaaaccgcac aaacagcacg
      661 ataaaggcga acatcgacac gacatacagt aaaattttgt cgcgctttgt catcggtgca
      721 atttccatgg cgcgcccctc cttactgcgg cgctgccaga atgcagcgca ggtcgatgga
      781 atagccgttg tcatcggcgg tgtagccggt gtagctcacg gtggaaaaga ccccgcagtt
      841 ttgcagggag cggatgtagg tcgggatgtc aatgacctgg ctggaattgg cgttgaacag
      901 cagctcgccg gtggaagcat tgtagctggt gaactggatg gtgatggacg cgccgcccgc
      961 cgcctcgatg cggtggagca gcgtgctggt cacgtcgggg tagctgttca gcaggttggt
     1021 catgccagtg gcgtagtccg tcaggctgcg gaactccgcg gcctcctcgg cgtccagggt
     1081 cgaggaaagg taggtggcgg agttgtcggc gcaccagttc agaatattct gccgccggct
     1141 tttcagcatc gaggtgtgca gtaccatcac gccccagccc gccatgcaga ccgccaccac
     1201 ggcgatgccg ggcacaacgg cgcgcagatc cttgctgcgg ctttccgtct tggcggcacc
     1261 ggcagctttt tgccagcgca gataaaagtt gttttgtttc attgccataa tcgataaatc
     1321 cccaaggcta tttatataag gtatcagcgg gcgatgagat tgccggcggc gtacagcacg
     1381 tcggccaggt gggtgccgct tggcagcttg acagtgggtg aatccgggaa aatctccacc
     1441 gccaggccca gggcctcaca cccggggcgg cagacggcaa aatcctccgc tgtggaaccg
     1501 gcaaagtaga ctgtgccgat ctggtgctcg ctcttgttgg cgatgtggaa ctgcagaata
     1561 cccgagagct tctgggcaat ctcggtgccg gactcgaccg agccgcgcgg gttgaacagg
     1621 cggctgcggg tggagtagct gtactgcccc cgcacaaaca gggtcgcaga gatcgtatca
     1681 tcgtcaaatt gcagcagaac aaaggtcttt tgctgcaaat cgggaatcgt gcgcacggct
     1741 ttgatggggg cggccagccc cagatcaatg caggtcagct tgaagcccgc cgccttggca
     1801 agggcatcgt acccggcaat gacgctctgc tccacgcggg tggccagcac ggtatcggtg
     1861 cgggtcttgg catcgcggga aagcagcata taatcatcca gcggggcttt tgtctcggca
     1921 ccgccggtgg aaagctcgtg ggaaagcacg gccattcgct ttttttcgct catggcgggc
     1981 agcgacagga tgcggtgcat aaactggctg ccgcccgcaa tcagcgccac gcggcttgca
     2041 ggcagtttgt tctgggcgaa aaacgccttc agcgcgtcgg ccaggtcagc ctcattggtg
     2101 ataacaccgt tgatcaggca gccctcgggc agctgcatct gcgcggcggc ggtaatgcgc
     2161 gcaccgcccg ccgtaggact gcccaccgcc gcatacaccg tgcggctgca aagatacaaa
     2221 gaggtaagtg ttgccatgtg gacctccgaa aaaattagtt taaatcgccc cggacgagct
     2281 gccgatggtg ccgtaggact ggtagatggg cacgataacg gccgccacaa cgccgccgac
     2341 cacaatgccc atgatcacga tcatcacagg ctccagcagc gtgaccaggc gcttggttgt
     2401 catttcggcc tcatagtcca gcgtctcact gatggtcgcc agcaggctgt ccagcctgcc
     2461 ggtctcttcg ccgacggcga tggacgacga gagctttgtc tcaaagccgt caatgcccgc
     2521 gatcgcctgg gacaagctgt ggcccgcacg gatgctgtcc agcgcgtcgc cgaactggct
     2581 ctcgatccag cggttgccga tggcgtcccg cgccgcgccc aacgttgtaa tgatggggac
     2641 gccgctgccg taaagattcg acaaagtgct ggcaaaacgg gccgtgcaga tcttgcggtt
     2701 cagcacaccc acgaccggcg cgtgcaggat aaagcggtcc cactggcggc ggaccttcgg
     2761 ctgggccagc aaaatgcggc cgcccaaaat cagcagcagc acgaccacga taagcaccgg
     2821 ccagttcgca cccacaaagt cgctgatgtc aaacagcagc tgggtcaaaa acggcagcgt
     2881 ctccatctgg tcaaacatcg gcttgaactg cggcagcaca aatgtcatca aaatcgcaat
     2941 gactgccacg ataagcacgc ccaaaatgat ggggtacatc aggctgttgc ctacctgctg
     3001 gttcatcttg tgctcgcgct cgtagtaggt cgccatacgc atacaggcgt ggtcgatgct
     3061 gccggtgccc tcggcgctgc ggatcatcgc cagcagcagg ttcgggaacg ccggagcgca
     3121 ggactccatc gcgtcggaca gcgggacgcc cttgcgcagt tcgcccaaaa tcgcctcata
     3181 cagggctttt tggcgggcgt caatgttgcg ctcatccgcc atgatgcgga tagcgcgtac
     3241 cagcggcaca ccggcggcca gcagcgtgcc caggttgcgg cagaaatccg ccagcatggc
     3301 ggttttcagc gggcggaagg cggagtggct tttctgggcc tcctgcgcgt cggtcatgta
     3361 caggccgtcg ccgcgcaatt tggtgtacag cgcctcctcg ctgggggcgt cggtcacgcc
     3421 gcggacggtc ttgccctggg cattggtggc agtatagcgg aaacgtggca taggggagaa
     3481 acctccgaag gatagagtgg cagggggcgg cgtcctcgac gccccgcccc tgcgctgcat
     3541 tacaaaaatt gacaatacca ccccagcacc ggctcaccaa acagcatggc aagcacgatg
     3601 ccgacgcaga gaaacgggcc gaaggcgaaa tggtcttttt tatcggcttt tttggcggcc
     3661 agcaggtaca taccgtacac gccgccgccc aggatgccga aaaacatcgc cagcagggta
     3721 ttctgccagc ccaaaaacag cccggcggct gccatcagtt tgatatcgcc gccgccgaac
     3781 gcaccgtcaa tggcaaggca gagcaaaaac atcggcacgc tgacgcagac tgcaccgatg
     3841 atatgccccc gccacccggc gggggagagc gccgtgcctg ccacgccgca gaccgccagc
     3901 gcgaaattca gccgatcggg gatggtctgc gtctcggcat cgatgagcgc aatgcttaaa
     3961 agaatgccgc agaccgccag cgccagcaac gccgcccagc tcatgccgaa cagtccgtcg
     4021 ggcagagcaa gggccgcgcc gtactgccgg gtacacccca gcgccagcac gccgcccaaa
     4081 acttccacca gcagataccg cgccggaata tgtgcgccgc agtggcggca tcgcccgcgc
     4141 aggaacagcc agctgaacac cgggaccaga tccggcgcgt ccagcgtgcg gccgcaccgg
     4201 gggcagatgc tgcggccttt cagcgggtcc atgttccggg gcagccgcca ggccacaaca
     4261 ttcataaagc tgaacagcgc cgcgcccagc agaaaccgga tcagacccag caggcactca
     4321 agaagcagca tggccgacct ccgcgctgta ttgcagccga tcctccgcgc ggaacacgcg
     4381 gtagccctcg gcggcgtcgt agatggcgta cataccgttt tcgcagtaga gcagtttttc
     4441 caccgtgtag ccgttgccgc cgatctgcaa aagcgtcacg gtaccgggca gtgcgcccag
     4501 tgtctcaatg gcatcggcca caccgtcggc aaagccgtca gggctggtct ggtcggcata
     4561 ggggcggcct gtggcgtaat actgggccga tacggccccc gccgccagcc gagccgcctt
     4621 gacctgggcc agcgcctccc gggcgcgggc ctggtagcgc agctctgccg cgccggtgac
     4681 gaccgtatag ataaccgccg ccaatgccag cgcaacagcc agtttttccg agaaggcggc
     4741 cttggcccac cgttccccgg ttttgcgcag ccatgcgccc agtcgtgctg ccagcatcgt
     4801 ttcacccctg tactttatat aataacgcac tattcagtat accgtggaag gacactattt
     4861 gcaagaaaat ttgcaaaatt tccccatttt ttgttgcaaa gtaacgcaaa atgggacgaa
     4921 tccatgcgga tattgccgca gaccacaata tttggtgtga tttttttgaa atatcgcact
     4981 tccttgttgc gaaaacgtca caggcttagt ataataacat catatatagt ctgttattgt
     5041 gtctatctgt caggattttt ttagatatac agggagcatc tatgctgcac caacccaaaa
     5101 acaaaaagcg caccggcggt tttacgatgg tagagcttat ggtcgtgctg gccattatgg
     5161 gcatattggc tgcgctggtg ggcggcggcc ttatcgccta cactcgcctg gcccggtttg
     5221 agaaaaacga ggccaacgcc cgcacgctgt tccaggcggc gcaaatctcg ctgacccgca
     5281 tggatactgc gggcgagctg gatgcgttct gcgccgatgt cgaggatcag ggcaaggcgg
     5341 gcacgcactt taccgatgat gttgtcatca ccgatgccgc cgggaatgag gtgcgccgcc
     5401 gcacggccag tgagctgaac aacggcatct acgcgctgta ctatgataag accgacggca
     5461 cgggccagaa cgagctggtc cggcggctgc tggacgatta tatctacgat gcttcgatgt
     5521 ttaacgccgc catctgcgtg gagatcgacc gggccagcgg gcaggtgtac tcggtgttct
     5581 atgacacgaa ctccgacaag ctgcgctttg ccgcggacgg cgtggccgac gcaaccgata
     5641 tcaccgaccg cagctacgac caccgccgca atgacagcct ggtgggttac tactcggccg
     5701 aggaccgcgt caacgtggtg gaattgcagc agacgaagct gaaagtcaag aacccgcgcc
     5761 tgtccaacgg cgagacgctg accctgagct ggggcggcaa ctccaacctg gccgacatgg
     5821 acactgtcta cacggcagtg gcctaccggg ccgagaaaaa cggcctgacc ggctacaaaa
     5881 ttgccgatga atcgaagccg ctgttctcca tcgagatcaa gcgccccaac accgaacagc
     5941 aggtgctggc ggaactggaa accaccgtct acacctacag cgccgacggc acaaggaccg
     6001 agactaagcg caccctgcgc tatccgctga gctacaacaa gggcaacttt gtcctgacgc
     6061 tggacgccat ggccgatgcc tcgttgctgc gcgcctgtga gaaagatacc agcaccgccg
     6121 acaacgacct gtacagcatc atccgcttgc tggccgagcc ggagaatatc atcatccgtt
     6181 tgcaggctgc cccgcgggac gagtatgccg acagctacac cgccagcaag agcgaggact
     6241 ccaacgccga gaacacgctg tttgccatcg acggcaccaa aaccgacgca gagctgaaat
     6301 atttccgcca cctttacaat gtccgctggg ccaaaaactg gggcgatgcg aaagccacct
     6361 acaccctgaa agcccagggc atgggcgcca acggcctgaa ctggaccggc ggcggcgtca
     6421 ccgtctacac agcggcgggt gtgggcaaaa accccgaggc caaggtcccc agtcagaccg
     6481 acccggtggc ctggcccacc atccccgcgc tgggggaaaa tatcaccctg caaagcgaaa
     6541 cgactgtggc cggcacggcc cgcgtgccga ttctcaactt gcagctgcgc ggcaattcgg
     6601 tcgcggcagt tgacaagacg aatgagaacc cgctggaccg ctatgtaggc ctggtgggcg
     6661 agaacaatgg cacgatctcc cacattacgc tgcgcgacgc cgacgtgcag gtcaacaccg
     6721 aaatcaaggc gctgaccacc gggcagagcg tgcccgaggg cggtatccgc ctgacgaaca
     6781 cgacctactt ggccgaactg ccggaaggcg acaccgcccg ccgcgacggt gtgcgcgccg
     6841 tgggtgcgct gtgcggcgtc aacaccggca cactgcagga ctgcacgctg ctccacggca
     6901 ccaacaacgc ctacaaggcg caggtgctgg ccacgctgca ttttgacgac gatacgaccg
     6961 aaaagacccg catccctgac gctgcgaaca aatactacga gcacgagccg cagggcattg
     7021 gcggcctcgt gggcgttgcc atacccaaaa acgatgcagg caccaagctg gaaaacctga
     7081 ccgtgggcga gaacgttacg gtggcgggcc tgctggtgga cgacgatacc accgacacca
     7141 ccgacgaacc cggcgaagca ggggaaaagg cccgctatac cgctgtggcc gccgacgcgg
     7201 gcgacacgaa aacggccacc cgctggcgca gtgtcggcgt gggcggcgtg ttcggcacgc
     7261 tggacgcggc taacctgaaa accgacgata aaacggtcat tgccaatgca gccaatgtca
     7321 ccggcagtgg ctttgtgggc ggcattgcgg gcaacctgta cagcaccgat accgccgccg
     7381 acggcacgat aaagacaatt gtaaacaggc tcgccagcac cggcacggtc agcgcgggcg
     7441 tgaactatca gggcgacaac actcaaacca aacacagcct ggtgctgggc cagttcttcg
     7501 gcggcattgc gggctacacc caggatgtcg tgctggccaa ctgcaccagc accgcccgcg
     7561 gtacgatgac cgagaccgac ctgcgcagcc aggtggatgc aggctgcgcc agcgacggca
     7621 cgctgaacga caacagcccc ctcaagggcg atttcgtcgg cggccttgtg ggctttggca
     7681 aaaatatcct gctgcagaac agccgcaccg agaaaggcta tgtcctgggc agccgctttg
     7741 tcggcggcct ggcgggcggc ttcaccggca gcacgctcca cgcagacagc gccgccaacg
     7801 ccagtgatgt gttcggcaac cgctatgtgg gcggcatcgt gtcggtcaac ggaagcggca
     7861 gcatcatcca gaccagcacc aacagcggcc tcgttgcggg cctgggcaaa aatgcagcct
     7921 acgtcggcgg catcgtcggt gtcaacgacg ccacctgggg cgcgggcagc gccgcccagg
     7981 ccgaagaagt gcaacttgtc aactgcacca acagtatgtc cggcgacaat gccaccgaca
     8041 gccgccgcat ccgccttttg caggcattga gcacccacgc gggcatcacc gagtatgcca
     8101 actatgtggg cggcctggtg ggctataacg gcaccaaggg cagtgtgacc tgggaccggt
     8161 acggcacccc gacgctgggc gcgatcttgt acggcggcac cttcgtcggc ggcctggcgg
     8221 gctacaacga tgggaacgct gccatcacca acaccagcgg caagcccctg actatccgcg
     8281 gccagatcgt ggccgacggt gacgcggtgg gcggcctgat cggcctgaac accgcacccg
     8341 tcctgcccac cgccaccgtg gcggctaccc gcgtcgaggg cgtccacttt gtcggcggcg
     8401 tcatcggcgc aaatatgccg gtgggcagct tcaccgtgca ggacaccctc ggcaaggcgg
     8461 gcgcactgac caccacggtc acggcgggcc gtgtccgtgc ggacggcgtg gcaggcggca
     8521 tcatcggcta caaccgcctg ctggcagccc tgccgcagga cgtgacccat gcggccatgc
     8581 tgcccacctt cagcgacgac aatgtgctga ccgacagcac ggccgtgacc agcagcgaag
     8641 cggcggtctg ccttgaaaac ttcaccaata ccttcaacct cgaggccaac gcctacgtcg
     8701 gcggcatcct tggctataac gatgcagcga cgaatctgac catccgcaat gccgtcaacg
     8761 gcagcagcag caacgcgctg gccgtcggcg gtctgagcat gggcaccgac gacacagctc
     8821 accggctgta ccagggcgtc aagctgctga gcctgaacca gaactacgag ttcggcgaga
     8881 cgcgcggcta cctggcgggc ggcatcctcg gctttgccgc caaaaatacc acgctggaaa
     8941 actgtacgag ctacggcaac attgcccacg cctgcgcggc gggcggtctg accggctgga
     9001 acgacggcac gatcagcggc ggcatgacct gcgcttcgct gggcagccag caggcgggct
     9061 acctgtacct gggcggcatc gcgggcgtca acacccgcag catcgccaac gcggccccgg
     9121 atcagggcgt tgtgctgcgc ggcaatcagg tcatcggcgg tatcgcgggc atcaacatga
     9181 gcggcgcggc ggtcacgtat gaccaggccg ctacccccgc cgcgtcggtg gtcgctacct
     9241 actgcgcggg cggtgtggca ggtgtcaact gcggcaccat cacgctgggc agcactaccc
     9301 agctgaactt caccgtcacg gctacgacct acgcgggcgg catcgcgggc agcaacaacg
     9361 cccggggcag cacgcggggc cttatcaccg gcggcgtgcc gatagcccag gtcacggctg
     9421 cgacctacgc gggcggcgct gcgggcatca actatgccga tatcagcaat gtcaacaacc
     9481 aggccccggt gcgcgccaac gatacgctgg cgggcggcat tgcgggccag aacgcggcgg
     9541 gcggcgtcat tgacggctgc cgggatacga accgcgaagt ctacgccacc aacggcgtgg
     9601 cgggcggcat cgcgggcacc aacgcggcgg gtgcggccat ccgcaatgcg gaaatcacgg
     9661 cgggggccac catcaccgcc gccaacggcg cggcgggcgg ctttgtgtcg gagaacagcg
     9721 gcctgatcga gaacggcacg gtccgcggcg gtaccatcaa ctttaccggc ggcaccatcg
     9781 gcgcggtggc gacctacaac cgcaagggcg gcaccatccg cggcacggcg gtggcgcaga
     9841 acgccaaggt gcacctcaac ggcccggcca ccatcgtggg cggcattgcg ggccgcaatg
     9901 agccgggcga gggcacgacc gacgcggcat ggattgaaaa cgccgccgtc aacgcgggag
     9961 cactggactt aaacggcctg accgtcaacg caaacctcgt caccctgggc ggcgcagtgg
    10021 gcagcaacgc gggcaaggtc aagggcacca acgtcaccct cagcgtgacc gagggcgaca
    10081 aactggaacg atatcagaat ttgggcggcg tcgcaggcca aaacgacggt accctggata
    10141 cgtgtaccta tcagggcacg ctgggcctgg ccagcgccaa ggctgacggc gattttgcct
    10201 ggggtgcggc cagcagggac ggcaacacca tcggcggcat tgcgggtgtc aacaacggca
    10261 cggtgcagaa ctgcgcggtc ccggccatcg tattgcaggc gcgcggctcc aacaacgtaa
    10321 gctctttgca gaccgacgcg cagaagctgg acaatgccac ccacgtaggc ggcattgcgg
    10381 gccgcaacaa cggcaccatc agcgccagct atgtggcgac cgacgcaaac ggcaagagca
    10441 tcattgcggc gcggtacggc tttgtgggcg gcgtggcagg ctccaacagc ggcagcatca
    10501 caaacagcgg cagccagcag gcgcaggcgc ttgtcagcaa ggtcaacaac gagtggctcc
    10561 ccctgaacac cgacgacgcc aacacaggca tcaacgccat ggtggccgaa ctgacaaaga
    10621 cggacggtac ttacaccggc tttaaaggtg tggataccgt gggcgctcat gattacggct
    10681 atggcagtgt gtacctgacc gagaacaaaa cgaccacctc cggcggccag tgggggacga
    10741 cgaccaccac caaggcgggc ctggctgcca acgacctgac cgtcgtgctg cgcggcaata
    10801 gtaatgtgga taacaatgac agccagcgcg gcagcggcta cctgggcggc atcaccggct
    10861 ataactcagt gacgggggca atcaccgaca gcgctaccgg ccgttggttt gtctacggcg
    10921 ataacatcag caaggcatcc accatcggcg gcattgtcgg caccaatatt tccagcaaag
    10981 acctgaccac gctggtcaac tgcgcggcgg tgcgccggta tacacggctc agctcccaaa
    11041 aaaatgatga taacacgaca aatgaacata ctggcgctaa ggccacgcgc attgaggtcc
    11101 atgtcggcgg catcatcggc caccagggta acgaccgcga cgacctgtgg gcgctgagca
    11161 agtgcgtcaa ctacggcacg gtctacaaca gccgttccaa caacatcggc ggcatcgtct
    11221 cctactggac ggactatggc ggcacgatcg agtacagctt caacttcggc gacctgctga
    11281 caaatatgaa tgataattgg gacagctgcg gcaccatggg cggtatcgcg ggctactttg
    11341 atgcgcctgt caagaatacc tccgccaata tcctgcactg ccagaaccat ggctcaatga
    11401 agggaacgaa taataaaagt gcgaacgata tcggcggtat ctttggcaag gtgcagatgg
    11461 cagataagac cgaccccatg accatcaatg tatacgactg tgtcaatggc agtacggttg
    11521 aaatcaaagc acagtctatg gcggttggta ttttcggtta tctggggccg tgggatacgc
    11581 gcggctctac aatcgataat gttatcgtca atattgaccg ctgccgcaac tattgtacga
    11641 atatgaatgc cagctacaaa gtcggcatcg tcggcaaccg tggcggcggc ggcagaagta
    11701 cgcagcctac gacgctgaac aactgcttct cgctggtagg cggtgataac tggcacccgc
    11761 tggcctatga cagtcccgtt actgccgtag agaacatcaa gggttcaaat aactattata
    11821 ttgacgcata tagtaaggat ggcaagagcg cttggaacgc gcaagcctgg aatgaacgct
    11881 ggttgggtca cacaagtacc aatttgacaa actttacatg gtctggtgca gacagccgct
    11941 ttacggaaag catggaaacc aaaaactgcg gcggtcgtcg tttgaacctg ggcaaagaca
    12001 ccacagacga cagctatttt gccatgctgg cggtgccaaa caatacgaat gataacacaa
    12061 cgtatgacct gtcccgggtt gtctacaaca cggcttatat ccatgtagat gcaacggata
    12121 catggaaacg tacagtggaa acaaaagatg gcgatgtgtt gggcaatatc ctgctccgtt
    12181 ttaccgagac aaacgacagt atcaagggct caagcggcca gaacatcccc agcaatggcg
    12241 atattaccga tgaagtcatc caaaattatt ataagtatgt gttggaggcc aatgaaccga
    12301 accagatccc cgaatccatc acgatccagc gcagtgagga gactaaggac ggcaccggtg
    12361 cagtgtatgg ccgctatctc gtaagctgga ctgcccccaa gcaggacgcc gcccacggcc
    12421 cggccatgta ctataagctg gaagtgtaca agaccgacgc aagcggcaag gtgaccgatg
    12481 ccacccccat acacacggcc atggtctacg agacgaagta tacctttgat aacgacccgg
    12541 accgcgacgc ctggaccggc aactttgtcg tgcgcattac ccccatgaac agcaagggcg
    12601 gcggtacatc ccgcgattct gcggtgcaga ccttcgccca gacactgccc aagccggaaa
    12661 ttgaagtacg gctcaagcgt accggcacgg gcaacatata ctatcagaca gttgtgctga
    12721 caaactatga cgaatatgtc agaatcgcca atgaccagtg gaaagttgat tttaattatg
    12781 tggacggcag ctccggcagc tccggcagct ttagcaaagg caagagcgag cagaccatca
    12841 ccaacggctt ggacaatatc agaaccctga ctgctattgc taacccgagt accagcacag
    12901 cgccgttcat gcggtcggaa cagttcagcg agcgtaccgc cattgcaggc ccctttacaa
    12961 atccggataa gtacgatgag tggaacaccg gcctggcggc taagggcaat gtgaccgtca
    13021 caaatccgac tgttatagga acgacggtca atgacctgac tatcagcctg aatttgtcct
    13081 atgcaccgtt tgtgcgggat gataacaaga tgcaggaagc ccagcctatc taccgcgtca
    13141 tgctcgtcgg caaatacgtc ggcgacagtg agacgacggc cagcggcaca agcctgacag
    13201 gccagtatat cacgctggcc gcgcagcaga cccgcgttgg ggctgcggcc accaccgtga
    13261 ccttccgcaa tctgccgaag gaagccttca ccagcgacta cgaggatttg aaaatcgtcg
    13321 cggctcctgt cgaaagcggc atcggcccct cgtacagccg ttgggcagct accgaggagg
    13381 aagtcacgga ggcgattgcc cagcgcggca cagacccggt cgcctactat gacggcctgg
    13441 aaatcgtgcg caaggcaggc ggcgacggct acgagtacgc ccatatgacc gcgctgtact
    13501 ttgtaggcaa ggcgtacaat accaacgaca aagacaaatc ctataacgat gtccttgccc
    13561 ccagtcaggt caagacactg tcgaccggcg taaccaccct gcaagccccg acgctggacc
    13621 aacagctgac gaaggaagaa gtgcgtaccg ccgaaaacaa gctgtactat acctttagct
    13681 ggacccagac aactgacggc acgataccgg atacaacagt gcaaaagtac accatcgagc
    13741 tgtacggcgt gcaggccgac ggcaagcagg agaccatccg cctggcaagc ggcgtggatt
    13801 tggctgacag agtcaagttt gacaatgcag gccgctacac gctggaactc tgcgtggacg
    13861 acgacctggc cgagggcagc aagagctgga agtacaataa cgtcattctc catgtggccc
    13921 gcgtgtccga cacgaacgcg cagatcggcc tggcggacga gcagagctac gccgtcatga
    13981 cccggctgga tgcccccgac gcccccggcg gatttacccg cctgtatgac gaaaacagcg
    14041 ccgatgcact gacatacagc atcaactggc ctgcggtcgt gggtgcggat cattacaccc
    14101 tgtacgccaa gcgccagaac gccgatacca cctgggaaac cgtggagacc tgggagaata
    14161 tcacaggcac caagaccgaa atcgacatgg agcagtacca gggccaggaa ctgcgcttct
    14221 acgttattgc caaccgtgcg gcgggtgaca ccaccggctt tgacagcccg gacggcgcac
    14281 tctgcgaccc gcaggctgtg aagtcccgcg tgacggcccc gacggtgagc agtgcggtct
    14341 ttgccccggc tgcgcccagc caggcggagt tcctgaatgg cgaaacgctg caaatgatgg
    14401 tcagcggcaa cacggccagc agctacttct ttaccggcta tctgtttaaa aatgtggatg
    14461 actatcagac gattgccgcg ttggcaaatg actggcaaac taccgctgcc ttgggcaagg
    14521 ataaggcaga caagctggct gcattgcaga ctgcgctgaa tgaaatgctc aaaaacggtg
    14581 acgccatctg catcatcccc gaaagtgacc ggcagagcgg cggcggcacg cccgccgacg
    14641 gcagcggcaa cagcgcggcc tacaacctga gtaacggctt taccatgaag cccgagtatt
    14701 cccgctacta cctgctgcct gccctgcgcg ctatggcaga gaccagcagc ggcatggaat
    14761 cctccaactg gtactactac gtgggcgact atatgaacaa cccggcagcg atgcagctgc
    14821 ccgccatcaa gttggacgcc ccggcggcaa agctggataa tgtgaccgtg acccatgcgg
    14881 cccagctgta cgatagcggc agctacgagc gccccatggg cgaccccatc agcctggata
    14941 tcacccattt tgctgtgcag tggaaagcgg ccaaccgcta tccgtccatc ggcgagcttg
    15001 ccggcggtat ggtcagaaac ctgaccgacc actacaagtt caccgtgacg gcaaaagacg
    15061 gcggcgaaaa ctacacaatc gaaatgtgga cctatgacgc tgacagcgag gaaaagaagg
    15121 acgacggcac gccggagcac ctgcgcggcg aaatcaagac ggtaaagaaa accgtcacga
    15181 ttgacggcaa caactacgaa aaataccttg acccgcaaat cgaaaaggac gccgacggca
    15241 atgtgacccg cacctggtac gatttgagcc tgacgcccgc gacgaaggac ggcggaagcc
    15301 gcgataccaa caactgggtc agccagccgg taagcctgcg cggacgcatc acacagaaag
    15361 atagtgacgc acaaccttat tatgaggtcg cggacgccat tgccacactg caagtcgtgg
    15421 acgacgacgg cgaacccgcc taccgtgtga cgctgccgga tatgcaggat atcctcacga
    15481 ctggtgacaa actgcatagc tttacggaca gcattctggt tgagacactt gtacattcca
    15541 gcggtgacgg caagaccgtg ggcacgccag acgccgaagc tgtaaaaatc ggcgacgaga
    15601 cccagaccgt cgagctgatt gcagaagagc cgacgcccga cgaaaacacc gaaaccacac
    15661 aaccgaccga aacgcccgca gagccgcaaa acgcgccaca gcccgtggag ctgaccgaca
    15721 aggccgggta aactacaaca aaggaggtgg catcatgcgc cgaaaagcac gcgatatcct
    15781 gcgggaccag cagggccttt cgatgattct ggtgctctgc atcggtgcct tgtttgtggc
    15841 cctgtctgcg gcactggtgt acgcggcgtc cgtgttgacg gccaacgcca accgtcagct
    15901 tttggagcag gaggcctacc agctggccac cagtttttcc gacgtgctgg aaggggaact
    15961 gaacaccccc acaagctcct tcgccgcgtt cgtgaatgac agcttcatga cctccacggc
    16021 ctacggcggc gatatctacg gcagcccctc gcagccctac tcctacacat ggaaagctgc
    16081 cgatgccgac acgagcggtg cggaggaaat caccgtcacg ctgcgccgcc gcccgggcga
    16141 ggacgctgaa aagctgaacc tgagcggcgt gggccagtcc gtcagcacca aaagcggctg
    16201 gcgcgaccaa ctgaccagtt ggcagagcaa cgactatctg ctgaccgatc tgcagctgga
    16261 cgtgatcgtg accgccacca aggcggggga gagctttgcc tataccgtga cctatgaccg
    16321 gcaggtacac tacccggtgt ctggctacac atttgacgat gatacgacgg tttacacatg
    16381 gagcaagggc acaaacactt ttgccagccc cggcttgtca agtatcgata ttaaaccgga
    16441 ggacgcagag cgaatcatcg tgccccactt tgacaccacc aacccggata agtccaccat
    16501 ccattacacc cgcggtgccc gccagagcac cagcacagca caggagtgag ccgccatgaa
    16561 acacaaattg caaaatcagc gcggcgagac tattgtcgag gtcatcgtct cctttatgct
    16621 gctgttgatg ttcacagcca tgtttgtggt gtcgctgcgg tacgcccggg ccatgtccca
    16681 aaaggccgag acgctgcggg agacggctta cgctttctgc gccgggctgt acccgcagga
    16741 aaaacagacc cccgcctggc agaccgaaaa aaccgggcag agctttacct tcagcgcggg
    16801 cgcgggggct ggggcgttca ccgtggacgg tgtggaactg gaccaggtgg agacccaagc
    16861 cgaagtgagc gagaacggcg gcacgcccac caccgagacg cacaccttcc accgttacgc
    16921 caaggaggca ggggaggcaa cgccatgaag cagctgcaaa acaagcgcgg caccacgctg
    16981 gtggagctga tcgtctgtat ggtgctgctg tccatcctga cgctggcggc ggtcacgctg
    17041 atacgcccca gcgcccaggc ctaccgggat atccagctgc agacccgcgc ccagaatctg
    17101 gccgatgccc tcatcgagac gatacgcggc gaggtgctag acgccaacgg ctatatccgc
    17161 tttacaaacg gcgcgacgga cagcgcaaat ctggacagcg tgtttgacgc ccagaccagt
    17221 tacagcgacg gcacggcgct ggaattcagc gtctacccga accatgtgga gctgatcgac
    17281 aaggaccttg tacccgcact gaaaaacagc aagggcaagg acttgttgac ccaggcccag
    17341 gccgaggaac tgaacggcta cctgcatatg cggttttacc agcaggagca gagtgatttc
    17401 gcccccctgc acaagaagga cggcgagaag atcgcctacg cctacaccac cgcctacccg
    17461 aaggaatcct acatggggct gtacatcagc gacctgcact tttacgcccg cagctgggca
    17521 caggaaaacg acaccgacac cccgcgtatc acggcgatga cggtggtcat cacagtagcc
    17581 aagcgggaca gcagcggcaa tgatgtcccc ctgtgtaccc aaaaggccat tgtgccgctg
    17641 cccggcgaac cggtgatttt gtacgcgccc ggcacctgga atgggaaggc atcctcagaa
    17701 cgataagaga ctttttgcca tattataaca aaatgtgaca aaccactgga caaatgccgg
    17761 acggcttgtt atactatgaa acatagagac gtgcagcagg tcgctgccaa aataaagggg
    17821 gtacaggatg gcaaccattg aaatcagaat gttgggcgat ttcgagattc gcgtcaacgg
    17881 caaaccggta ttggcggtgc tggggcagtc gcgtaagact accgcgctgg tgcagtatct
    17941 ggtgctgcag cggggtgagc gcgtaaccca taaagtcctc accgatacgc tgtgggcggg
    18001 ggaacggtcc agtaacccgg atatggcgct gcgtgccatc ctgcaccgtt tccgcaatat
    18061 gatcgaggcc gagggcctgg acgagctgcg gaactgcatc ctgaccagcc gcggctgcta
    18121 ccagtggaac ccccacctgg actgcaccgt ggatgtgttc cgagtcggcg acctgtccga
    18181 gcaggcccgc cgcgaggagg accccgaaaa acgcgctaag ctgtatgagc agatcgttga
    18241 tctgtaccgc ggccgcctgc tgccccagtc tgcgtccgag cagtgggtcg agggcgtcag
    18301 cgtgcggctg catggccagt atcgcaccgc catgttgagc ctgctggagc tctgcaaaaa
    18361 gtggggcgaa agcacccgca ttatctcgct gtgcaaccac gccctggaac tggaccccta
    18421 cgaggagcgc ctgtatctgg aagaaatcgt cgcgctggaa gccctgggcc gccataacga
    18481 agcccagaaa ctggcccgcc gcggtactgc catgggctgc ctgcaccatg ccattgagcc
    18541 gggccgcgcc ggttcagctt accgccagat gcgccaggct gaccgcaata tggaaggcga
    18601 ggtcgccaag ctggtggctg ctctgcccga caccgagaac gccggggcct gcatctgcga
    18661 tttcgagacg ttcaaagaag cctaccgtgt gcagcgcggc gtgcaggccc gctacggcct
    18721 gccggtgttc ctggcaatca tgacggtggc ccccggtcag gatgccgaac ccgacgagac
    18781 ctccacgatg gtggaccagc tgggcgagct catccacacg agcctgcgcc agtgcgatgt
    18841 ggccgcccgc tataccgaga cgcagtatgt catcatgctg tgcggcagtg ccgccgagac
    18901 cgggtccagc ccgctggaac gcatcaaggc gtcgttctac cgcgtgcccg cccatggccg
    18961 ttacctgctc agctacagcc tgtacgtgcc cgaagtcaag cccgacggcg gccgtgcccg
    19021 ccgcaagcgc agcaaaaaac agtaaaatat ttccgcccag ccgcaaaaaa gtttcgcggc
    19081 tgggcggctt tttttgattt tttacacctt ccacaatctt ttgcctaaaa ttttttacaa
    19141 aaaatccgaa aaaactgttt aaatttccat gataatgtgg taaagtagta tcaaacaggc
    19201 gggagtgttc aaaaatcgct ccgccgcaaa gaatatctac gccgcagggc agttggaaat
    19261 gccccggcgc gaaaagaggt ttgacgatcc atgggctacg caagcaaaga catccgcaac
    19321 attctggtgg ccggccacgc cggctgcggc aaaacgacgc tgaccgaggc gctgctgtac
    19381 atgagcggcg cgaccgagcg tatgggccgc gtcgaggacg gcaccaccgc cagcgacttt
    19441 gaccccgagg agatccgccg caaggcatcg ctgaattcca gcgtcgtgcc ggttgagttc
    19501 gagggtgtca agtacaacct gatcgacgca ccgggtctgt ttgactttga gaccggcgct
    19561 gccgagggca tcatggctgc cgagagcgtg ctgatttgtg tttccggccg ttccggtgtc
    19621 accgtcggtg ccgagaaagc ctaccagatg gcgctgaaaa acaacaaggc gcggatgatt
    19681 tttgtcacca agaccgactt ggagaacgcc gactacttca agattctgga gcagatgaaa
    19741 attaagttcg gccccagtgt ctgcccctgc gttgtgccag tccgtctgga cgacggcact
    19801 gtcgcctaca tcaacctgtt cagccagaag gccttcaagt acgagggcgg caagcagatt
    19861 cagatcgacg tgcctgatat cggccaccgc tttgagggtc tgatccaggc catgagcgag
    19921 gccatcgccg aaaccgacga ggccctgatg gagaaattct tcgagggcga ggccttcacc
    19981 accgaggaga tcgtccaggg catggccgca ggcgtgcgca gcgggcaaat tacccctgtg
    20041 ttctgcggct ctgccgtcaa cggccaggca ctggatatgc tgatttataa catgaacgtg
    20101 ctgctgcccg gcgcggatac cgcctccgct gtgggtgagg cgaacggcga accggtggag
    20161 atcaccgccg accccgctgc cccgctgtgc gcctacgtgt tcaagactgt ggcggatccc
    20221 ttcgtcggca agctgagctt tatcaaggtt ctgtccggca agctgaccgc caccagcaac
    20281 gccatcaacg cccgcaccgg ccagccggag cgcctgggca agacactgac tgtctgcggc
    20341 aaaaagcaga ccgacacccc cgagatcgct gccggtgaca tcggcgcggt cgccaagctg
    20401 gctaccgcca agaccggcga taccctctgc gacccggccc gcgtcgtcgc gctgcccgcg
    20461 ccggtctacc ccatcagctg ctaccgcatg gccgtcaagg tcgccaagaa gggcgacgag
    20521 ggcaaggtcg gcggtgcgct gacccgcctc atcgaggagg acccgtctat caaatttgag
    20581 gtcgatcccg agacgaagca gcagatcatc agcggcctgg gcacccagca tctggaagtg
    20641 gcccttgcca agctgaagaa taagttcggc gtggagatca cgctggagac cccgcgcgtg
    20701 ccttaccgcg agtctatccg caagagctgc aaggcccagg gccgccacaa gaaacagacc
    20761 ggcggccacg gccagttcgg cgacgtctgg atccagttcg agcctgtgca gggcgaggcc
    20821 attgagtttg ccgagaacgt gttcggcggc agcgtgccga agaacttctt ccccgctgtt
    20881 gaaaagggcg tgcgccaggc ggccgagcac ggtgtgctgg ccggttatcc gatggtcggc
    20941 atgaaggcca ccctgctgga cggcagctat cacccggtcg attccagcga aatggcgttc
    21001 atcatggccg ctaagctggc ctacaaggcc gctatccccg aggccggccc tgtcctgctg
    21061 gagcccatcg gcacactgaa agctcatgtc ccggcggaca acaccggcga tatcatgggc
    21121 gaagtcacga agcgccgcgg ccgcgtgctg ggcatgaacc ccgatgacga cggcctgcag
    21181 gtcgttgagg cggaagtgcc catggccgag atgcaggact tcaccacctt cctgcgtcag
    21241 ctgacccagg gccgcgggta ctacacattc gagttcgtgc gatacgagac cctgccccag
    21301 atgctcgaga gcaaagtcat cgagaaggcc aaggcgctgg gcaactttgc agatgaggat
    21361 taaagcgtaa aaaataagca cctgctgacc ggtatgcgtg tgaccggtca gcaggtgttt
    21421 tgttgtatgg cgttgtaggg ggcggcgtcc tcgacgcccc gcgggcaaag catgaatccc
    21481 cctgtcaggt aatcttcagc gcccgttgca cccgatacac aatatatcgg aacagcccga
    21541 cgatcagact gccctccacc caaaaatagt tgggcaggtt gcgccagcgg aacttcggct
    21601 tctgtatctt cggcagggcg acccaggctt cgtgaaaacc cttggcgtag tcattgccga
    21661 acccgcgcag ccggaacatc aaaactttca gcagataccc cagcagcatc gggataaagt
    21721 tgatgaccag catcaaaagc ggctggtttt tgtagggcag caaaatactg ttgcgcccgc
    21781 tctggatgct cttgaacgga ttgtaccgca ccgcaccggt cgtcgcgccg cagatgtggc
    21841 ggcacttggc cgtggggcag tagacgttct tgtagcccag gctgttcgcg cgccagctca
    21901 ggtcaacatc ctcatagtag gcgaaaaaca gttcgtcaaa tacgccgatt tcctctaaaa
    21961 tcgatttgcg gtagagcgcc gcaccgccgc aggcactgaa aatgcggcag ggcttggtgt
    22021 agcggctggc tttcaggccg tcgccgcgct tgcaggcaaa acccaaaatc gtcacatagt
    22081 cgcccgcgtc gtcggccagc tccggctcat aaaagcgcag catcaggctg ctgacggcaa
    22141 aaatcttcgg gtcggcgtca gcggtcctga taagctccgc cagccagtcc ggctcggcaa
    22201 aggcgtcgtt gttgaacagc accgcgtact cgcccttggc aatcgcaatg ccctggttga
    22261 ctgcgtggga aaatccggtg ttctcggcgt tctcaatcag tgtgtagcgg tcacggccgc
    22321 ggtagctgcg ggcaatttcc aggctctcgt ctttagagcc gttgtcaatg acgatcagct
    22381 caaagtcctg ctcggtctgt gcccagaccg actcgatgga atcccgcagc cagcccgcgc
    22441 cgttcagatt cgggatgata acagatgctt tcatggcgtc tcctcagcgg tagtaagggg
    22501 tggcgtagta gtaggccagc tggctgtgca ccacggcgct gaacaccagc gcggccacca
    22561 gcaccagcag cgccgcggct gccgccacga ccagcgtgcg gacgacctgc gcccgggcca
    22621 gccgcttgcg ggcctcgttg tgggtgccgc caaagctcca caccagcgcc aggatgaacc
    22681 ccacgaccgg gatggcaaac aaaatcatcg tgccggtggt cgcgccctgg ctcatggctt
    22741 ctgccatcgg ctcggcctcg cggtcgtcgg gcgtgccata cagcggcgcg gcgggaccgg
    22801 gctgggcggc ggggcgtacc gccagcgggg ccggggcacc gcactggggg cagcggtcag
    22861 cgtcgtgcgg cagcagcagg ccgcagtttt tgcagtaacg taacatcttt acctcgttat
    22921 tcggccagcg tcagattgcg gtccagctgg gtgttcagga tgatctgcgt gttggtggaa
    22981 cccatcttct gcatcttggt cagcaggtgt tccagtgcgt caatgtcggt gcagctgacc
    23041 tttatgataa agttgtagct gccggtcacg cggaagcatt gcagcacctg ggattcctgc
    23101 tcgatcatcg ccagaaagct ggcgcgggtc gcggcgtcca ccagcaccga gatcagcgcc
    23161 tcgacccggg cgggggcgtc tgggtggcgc agcaccacgg tgtacccgcc gatgatgccc
    23221 tcctgttcca gccggtggat gcggctggaa acagccgggc tggtcagatt gacctgctgg
    23281 gcaatgtctt tgactggcat acgtgcattt tccgccagca gctgtataat tttgtggtcg
    23341 agatcatcca tgagttgact ccttgttttt cttttgtata atgcgcacaa aaaaataaag
    23401 tgaagtatac agtcattgtt atctttaaca aaattgaaaa agtatgcttt tttctgcgcc
    23461 tgctaaataa aattaagcta tcgtggtttt attataatct tttgaaaacc aaaatgcaag
    23521 tatgacattt gacattttaa gaaaacttgc tataatgata tttgtaatca accaagacat
    23581 tcactgacct ttat
//
LOCUS       DBMDSQ010000004        22093 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_797_length_22093_cov_1.935795, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000004 DBMDSQ010000000
VERSION     DBMDSQ010000004.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 22093)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 22093)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22093
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_797_length_22093_cov_1.935795"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1402
                     /locus_tag="ACZPAX_00285"
     CDS             <1..1402
                     /locus_tag="ACZPAX_00285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1112365.1"
                     /translation="KFRTLGRREKKSLVAVNLVVLLIVWPGIWGNDDLAVLSLARTLQ
                     PNSWQHFLTSGAFILSLMFVPMPGGVVLVQNLLISAIVGSFAAAAQDLTEKRLGHAVK
                     PAWFAIVYLPFLLPPVVMHTQQPFRTTWSGWTELFLVFMLVAMYLRGTKLNKKELCAI
                     VVLGTLAVSWRSECVYYLAAIPVLLLVMCARKLLRPTAVAAATVLVFAGYLACSRYTS
                     TLMGEAWQYKMIALCYQTASLVQDADPVADADELAAIDRVFDVTYCRENSNIHGPEMR
                     TNMTRETGVTEEDWSACQKAFFKLALKYPKSLLRERMGVFYNTLRQRKTGMSNQKIVF
                     SSSFLLYDAEPTKADQKEFFADSVAVQPLNKELRRAFIIKVANGDGIIDWTWWMLPPF
                     VLLFAAVIVLLVQRKWMLFFAAGTFFARIPLVFLTAPDTYFLYYLTPFIAGYAVAAAG
                     VLYAVMKHKSKRGRLL"
     gene            1399..3030
                     /locus_tag="ACZPAX_00290"
     CDS             1399..3030
                     /locus_tag="ACZPAX_00290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1112366.1"
                     /translation="MMKRKKHTLWLELGGAVLWCLVTLFTDKLFFWYDWHSWRFFAYK
                     ALFILAAFGVIHGVVTLVQKVREKDSFTLRWLRWTLPYLAVTLLLLLVLWPGIWGNDD
                     KTILDYARTLEPFAWHHFLTSVWFIISLMFLPMVGGIVLIQTILIAGIVGLFIAEAQT
                     LAEQRLQKPLKSGWFVLLYLPFLLPPVVMHDMQLFRMVYSNWVELLVLFLLVRWYLRN
                     TAIKRWELVLFAVLGAVAAAWRSEAIYYAAALTILLLILMRKGLLKPIAAGAASAVIV
                     VGALACSRYNASLMGNDLQYQTLALCSQAAALVQDADPVSDVEEFAMIDNVYSVQKCR
                     ENSNLHKSDLFGAVVQPNLTEEGWSACKKGIVKLALKYPKSLLRERLGMFRATMQADY
                     GGSRQKDFFGFAYVCYDLDGYYLTSIERAGKIAYQSPLAFPINQDLRKAVIGTMVYDT
                     ETPLGKLISTTWFMLPPLLLLFVEALVLAVRKKWFLFLVTGTLCLRVVLVFLTAPDSF
                     FMYYLTPYIAGYAIAAAGVVYEVMRRKLKVERNPG"
     gene            3030..4973
                     /locus_tag="ACZPAX_00295"
     CDS             3030..4973
                     /locus_tag="ACZPAX_00295"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015651.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HIR1112367.1"
                     /translation="MRTLYLLLALALVLVGHTFRLLRWEQFIRIYERPMRGRMLRGMA
                     GGYALNFVLPFHLGDVFRAVFTGRRMKSGIGFSLATVIMDRFLDVWFVALGFIGFWAA
                     GFGGEVVHRAARYYLVFSLVLAVGLVLVLLLRDWLKKICLAFCGIFNDTLKLDGMMFF
                     WSLINTFKDLGRVKIGRLVVNTLVMWAAYLGSYGALAAALTAAGQPMQLVDVFDLLFG
                     RNAADLAALLPGGMLGGAAPMVRGLLVLWSVLPLVIMWAATLLPETVRGAVNHATQAA
                     PVGENYLNLLPQADEHDRAAFLSKYFGLENKDYVKRFLQMNRDITILQDYSAGSNATT
                     MLCMDKETTFYRKYAFGKDGDKLAEQLDWLRAQQSRLPLCDILRSGEDDGYCWYDMTY
                     SPQAVGMFRYLHSNPVANSQRILREVLDTLEEKLYKPTACPADAEKIERYIETKVDGN
                     LKKLHDDRILRELMGYDTLRINGTTYRNLNQLGWMFDHERLRKLFAGDPVGTIHGDLT
                     IENIICRTDTDGWYLIDPNTGNLHESPFLDYGKLLQSLHGSYEFMMKTPRVTVQDDRI
                     DFQLTRSAAYDALLTAMLDDLKARYPREQVESILMHEVVHWLRLMPYKLAKDRKRAPM
                     FYAGLVMVANDVADFVQGEGKLC"
     gene            4967..5524
                     /locus_tag="ACZPAX_00300"
     CDS             4967..5524
                     /locus_tag="ACZPAX_00300"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR1112368.1"
                     /translation="MLICVDLDGTLLDTVPANAASYRAALEELGFTVTDEYYAERCNG
                     GHYTRFLPPLMGGTPSTADVERVHDRKKALYSDFLDVVRPNTALMEILRTMQAAGHDL
                     ACVTTGSKQNATEVLEHFGVRELFGLIVTGEDVEKQKPDPEGYCKAMAHFRVTPAETM
                     IFEDSGIGLEAAKASGATVFRVEQF"
     gene            complement(5744..6700)
                     /locus_tag="ACZPAX_00305"
     CDS             complement(5744..6700)
                     /locus_tag="ACZPAX_00305"
                     /EC_number="2.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HIR1112369.1"
                     /translation="MADIKKIATRVSYGNTLVELAQQGADNLVVFDADLAAATKTEIF
                     RKAYPERHFDCGIAEQNMVGVAAGMSTMGYVPFVSSFAMFVAGRGFEQIRNSIGYPHL
                     NVKIAATHAGLSVGEDGASHQCCEDIALMRSIPGMVVISPADDVEARAAVIAAYNYQG
                     PVYLRFSRLATPVFHDPETYEFEIGKGEKLTDGYDIAVISTGLMTNEALRAAVLAKRQ
                     GMNVRVINMPTIKPIDEEIILTAARECGRIITVEEHSVIGGLGEAVCAVVSEKLPCVV
                     RRIGVQDQFGHSGPANEVLRDYGLSAENIVNVIREMVRPENK"
     gene            complement(6693..7562)
                     /locus_tag="ACZPAX_00310"
     CDS             complement(6693..7562)
                     /locus_tag="ACZPAX_00310"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HIR1112370.1"
                     /translation="MTKLETLQLKLTATRVRMGVIEATHGAGCGHPGGSLSAADVLTY
                     LYFREMRIDPEQPQDPNRDRFVLSKGHCAPGLYATLAERGFFPVADLPTLRHSNSYLQ
                     GHPNMNTVPGVDMSTGSLGQGVSAACGMALAAKQSGSDINVYTLLGDGEIEEGECWEA
                     FMFANHYKLDNLCIMVDVNGLQIDGATRDVMNSEPLDKKMDAFGFNTIVCNGNSFSDL
                     EQAFKMFDLSHGSGKPTCFLLRTTKGLGVSYMENAVDWHGKAPNDEEYAQAMTELRAA
                     HASLEKEIEFNNG"
     gene            7921..8073
                     /locus_tag="ACZPAX_00315"
     CDS             7921..8073
                     /locus_tag="ACZPAX_00315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1112371.1"
                     /translation="MPNGIYIQTEYHGKLIRKIVCNGDERWFIGSNCAVTFLSMTDCM
                     AAIDRL"
     gene            8149..9705
                     /gene="cls"
                     /locus_tag="ACZPAX_00320"
     CDS             8149..9705
                     /gene="cls"
                     /locus_tag="ACZPAX_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009322559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HIR1112372.1"
                     /translation="MKKRNWRLMLRRVLNLVFSRLVITVTLVLLQVFWLFSVFHWLDA
                     YSTWLNGVGLALSVIMCLALIRQDSTAPEFKISWMIIFMLMPVQGGLLYLLWGNKRPA
                     LGLRHRLERAETAMAHTRREDPAAVAALRKQNPRAAETARYLHDYAPAPLCGDTAVKY
                     YPDGQSMFADMLPALQSAEHSIYVESFIIGMGEMWGQIHEILRQKAAAGLDVRVIYDD
                     AGCLSLLPHNYAEMMRADGIRAFSFNRCVPLLNLVMNNRDHRKIMVIDGKIGFTGGVN
                     LADEYINKLERFGYWKDSGVRLKGPGATALANIFLTFWKAQYPDEEIDMDRDLPLAAP
                     VKTDCLVQPFADSPVDREAVAKNVYLELINQAQRRLYICTPYLILDNDLLTCLRLAAK
                     RGVDVRIYTPGVPDKPTIYQLTRSYFPHLLRAGVKIYSYTPGFLHAKTWLVDDRIAAV
                     GTVNLDYRSLYLHFENSVLIYGGAVLEDVRKDLAEIERESKELTLGDCRTGFVGTMYS
                     AVLRLVAPLC"
     gene            complement(9752..10921)
                     /locus_tag="ACZPAX_00325"
     CDS             complement(9752..10921)
                     /locus_tag="ACZPAX_00325"
                     /inference="COORDINATES: protein motif:HMM:NF012311.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HIR1112373.1"
                     /translation="MKRFFLLTACLLALTACTAAQTAVPAASPAPTEAPAATPTTVPF
                     SVPAPYSDLSYLPELSWDAAHQKQTYQPGEYDRLSLNTYSFNDRTVLAGAEEETAALL
                     EAGRDPGLGVRSLQARSITGQGVNVAIIDQPLLTDHHEISDAIVDYYDAGGYTDEGTM
                     HGPAVASILAGKTIGVAPGAHIYYAVTPGTADSRPYADALHYILALNDTLPESKKIRA
                     VSVSANPGNANFFENAGLWQAALSDAEDAGLLVLTVQGASAGSARLVPGLAAFDPAQR
                     DTPAACRMGQPGAFLITPLARKNPNYVGVPCAYRTVAEEYISEQCGYRYDVQGGLSWG
                     IPYCVGVMALGWQVAPALTNEEMLALLVSTAAPTADGARMIDPVKFIEAVENRYT"
     gene            complement(10947..11324)
                     /locus_tag="ACZPAX_00330"
     CDS             complement(10947..11324)
                     /locus_tag="ACZPAX_00330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1112374.1"
                     /translation="MKCTKGIWLRCGLLPYRLRGQQLALALLLRDKPRTAAQLAAALT
                     EKGYPCDSQMLEKSLARLAKRGILSVSDGVYRCTSPKLDAAPYTENQLLDLVGMTQST
                     VNGTAFYTAAPPASVSSRGPSGS"
     gene            11544..11960
                     /locus_tag="ACZPAX_00335"
     CDS             11544..11960
                     /locus_tag="ACZPAX_00335"
                     /inference="COORDINATES: protein motif:HMM:NF016432.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HIR1112375.1"
                     /translation="MTYQEKMKWLGRYQAALSYQHMLEDEIEVLRSDAERVTTCMSGM
                     PGRGGPNVDRLPRAVERIEKAQKKLAQQLDSCLETRMEVMHSIMALSDTAEQEVLRRR
                     YVMGQSYTDIANAMGVVQRRVYQLHKAGVEKVELRS"
     gene            12103..12288
                     /locus_tag="ACZPAX_00340"
     CDS             12103..12288
                     /locus_tag="ACZPAX_00340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1112376.1"
                     /translation="MCEINEEHVLDELAEIAFATPGEEGGLPVKVADKLRALEMLYKH
                     LGLGDGAKDEGVVIVDE"
     gene            12322..13566
                     /locus_tag="ACZPAX_00345"
     CDS             12322..13566
                     /locus_tag="ACZPAX_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009324011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HIR1112377.1"
                     /translation="MKIRLKEKIPAVFWQVHQAIRRGEVTEVVAKGGRGSGKSSYLSM
                     ELVWQLLRHPDCHAVVLRKVGGTLRNSVYNQIVWAIGELGCAGYFRCTVSPMECTYLP
                     TGQKILFFGLDDPGKLKSLKLPFGAVGLCWFEELDQFDGPEEVRNVEQTVLRGGSWTL
                     TLKSFNPPAMARNWANRYALEQRPGKLVHHSTYRDLPQEMLGARFWADAEHLQRTNPA
                     AYRHEYGGEVVGSGAAVFENLQLRAVTDAEIERFDRVYYGVDWGWYPDPWAYNAVYYD
                     AARRTLVIFDELTRNRTPNRETAQLLVERGLTDPDSGTLTADAAEPKSCADYRAAGLP
                     CRAAQKGPGSVRQSMKWLQGLAAIVIDPVRCPATAAEFSEYEYDRDPRTGEVLAGYPD
                     VNNHHIDAVRYAVEGVWRRRGA"
     gene            13695..15026
                     /locus_tag="ACZPAX_00350"
     CDS             13695..15026
                     /locus_tag="ACZPAX_00350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1112378.1"
                     /translation="MRGEDELQSYLEQAFGKSDVTSVKMRAALREWLDLYYGTPRAGE
                     DAAPRLAALIVGKLCRTVFAEYETRLPENAGQPMRCSLAALNAIAKPAMQYALVGGEC
                     LLKPVPKDGAFDFVPIRRDCYVPLARDAHGRILAVGTMERHSCDGRQYALLERRTAGA
                     EGLTIETRLFELNGQALGRCVPLATLPACADLVPQLMLPGVQGVGLAVLRTPLMNCVD
                     GSADAVSIYAPAAGLLHALARCEEQLNTEFANGASRVFASEDLLRPDAQGRRALRDDL
                     FIGLPDDPANVGVTVYSPALREQSYLARKQDLLRGCESLLGLRRGILSETEANADPRT
                     ATEIAATSVDYDLTIRDLQGAWGAAVQDAMVLCGTLGALYGLEYHTDAVVPAIDWGDG
                     VLYDRARIWAEQREMVDAGLLRPELALAWYFDLPCGSEAELKEVREKFMPK"
     gene            15063..15221
                     /locus_tag="ACZPAX_00355"
     CDS             15063..15221
                     /locus_tag="ACZPAX_00355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1112379.1"
                     /translation="MDEQSRQTPAPYAAGTGSVPVAPDRAAFDRMGYRERLALKRENP
                     EVYQELKK"
     gene            15353..16207
                     /locus_tag="ACZPAX_00360"
     CDS             15353..16207
                     /locus_tag="ACZPAX_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007048289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N4-gp56 family major capsid protein"
                     /protein_id="HIR1112380.1"
                     /translation="MSDFITKLSDMIDPEVMGDMVSARIPKKLRVAPFAKIDDTLAGV
                     PGDTITVPAYTYIGDAADVAEGGEVAIEKMTTSTRKATIKKAMKGIGLTDEAVLSGYG
                     NPVGEANTQLALAIAAKIDSDCMDALQTASLVYDGSKENISYNAIVDAVDLFEEEMGC
                     SDKVLFIHPKQVTQLRKNPDFLSADKYTPGVSLTGEIGMIAGCRLVPSKKVPLTGGVY
                     ACPIVKLEADPEVDDEIPALTIYRKREVNIETERKPKTRTTEITADEFYVAVLSNEAK
                     VVLAKFKA"
     gene            16220..16591
                     /locus_tag="ACZPAX_00365"
     CDS             16220..16591
                     /locus_tag="ACZPAX_00365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HIR1112381.1"
                     /translation="MPDYTYYTEKYLGEDIPEAAFPRFARRAAEELARMQNIYPMAPC
                     KGIEPDTAYAMAVCSIADAMYEFAQEDEARGLAKVSVGSVSETYTAPPQLCAATLALR
                     AGHYRHEAGYYYEIGRWLPHA"
     gene            16584..16991
                     /locus_tag="ACZPAX_00370"
     CDS             16584..16991
                     /locus_tag="ACZPAX_00370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1112382.1"
                     /translation="MRKNRLCGETVTLYHPDLAAQTVCRRVLRGVSWQQGRREKPDAA
                     GTKQGTALLLVIPEAAGQYGADYTLDPGDRVLLGEGPGVAWADWPGFVPAAVAGLAVV
                     VYVLPLRLRGRVHHVEAGAWWNASGTGAHSLTR"
     gene            17166..17609
                     /locus_tag="ACZPAX_00375"
     CDS             17166..17609
                     /locus_tag="ACZPAX_00375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1112383.1"
                     /translation="MMQKLYAFLARAPALAGVTLAVGDAGPAPGTAGLWCKGVTVLEQ
                     RENLLGTVRQRCRAEFTLRLCLPLPPGDSATAAENAARLLALQTWVAAECAAGRAPVF
                     GNADPARETLRAEQGKLERADAGGTAVYSLRIRAEYTQLYTEETP"
     gene            17606..18082
                     /locus_tag="ACZPAX_00380"
     CDS             17606..18082
                     /locus_tag="ACZPAX_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007048285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1112384.1"
                     /translation="MKIERKYMAHYLNAAFGKGEADYTRLGQDLEEYAPELTANVEKK
                     SNILGQTSVVIDGYQKQGEVSPYYAEEGDPLFEKLQGIIDGDLVLDDLKTDIVEVKLW
                     SKDTAGAYPAVREECYIEIVSYGGDTTGYQIPFNIHYTGVKTKGTFDPASKKFTEA"
     gene            18086..18541
                     /locus_tag="ACZPAX_00385"
     CDS             18086..18541
                     /locus_tag="ACZPAX_00385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type proton ATPase subunit E"
                     /protein_id="HIR1112385.1"
                     /translation="MKKLNIDTGVEEFSVNGQGVLRFNPADPNLYHRFFAAGRTLNDY
                     DAALTKALQTVEGDEQACAAAGLRLLNEYDGKIKALLTGIFGPENDFDAILGGVNLAG
                     VGANGKRVVQNLLEALTPILREGAERRLKAAADTAEREAAAARAARGAV"
     gene            18538..19110
                     /locus_tag="ACZPAX_00390"
     CDS             18538..19110
                     /locus_tag="ACZPAX_00390"
                     /inference="COORDINATES: protein motif:HMM:NF018553.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gp15 family bacteriophage protein"
                     /protein_id="HIR1112386.1"
                     /translation="MTGPWTLPTQAALGGGAYVIRTDYRDILELLRWLGGQADPALDQ
                     AERWYVAMGLFYPEFDAMTQDVWPEATRFLAEFLAAGRREEPRPGPALMDWQQDAPLI
                     AAGIGRAAGQDVRTLPYLHWWSFLALFDAIGEGSFATVVAIRDKLRRGRRLENWELDF
                     YRTHRTLVELRPAATAEEQAEKQRLLAMLG"
     gene            19116..20642
                     /locus_tag="ACZPAX_00395"
     CDS             19116..20642
                     /locus_tag="ACZPAX_00395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_process: GO:0098003 - viral tail assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail family protein"
                     /protein_id="HIR1112387.1"
                     /translation="MNKTTNIIFDELSTLRLPSGSLQTALDKVAAALGSVGDAGQQAR
                     QVVDELRASLQAAAVQGVKSARSLAKFDEINRLSAPATEKTAASAKEKQAAQAAAKAA
                     KSTAGTTAGSTRRSGKAGTDAAEFSAVWQQVLTQLRGIWADFWVYLQGFFAPFVAAWQ
                     TVWAGLGAAVSSVWEPLWAELSTVATPAVELLDTVWQGLWQGVQNAWAVYGQPILEGL
                     AQGWQNVVSILSALWGGVVQPVFFQLFDLLAALWSAHLQPLWNELTACLGAVAALLLT
                     VWNTILAPLVQWLIATLAPVAVQVFTAVGIAVTGAAGIIADAITLALAVLRGLADFLT
                     AVLRGEWDAAWNAMAATVSTVWGRIVSIVQTAVSTVLGVVRSMVSAIAAAINGLLSAI
                     GRAKSMAGSVLSGAGQLLTAQAALPGLGYAASVPIPALAQGAVIPPNRRFLAMLGDQT
                     GGTNVEAPLSTIKQAVAETLAGWQGGDGQPINIYIGEELLDSVIANSQNRRALRSGGR
                     "
     gene            20646..20993
                     /locus_tag="ACZPAX_00400"
     CDS             20646..20993
                     /locus_tag="ACZPAX_00400"
                     /inference="COORDINATES: protein motif:HMM:NF042749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6711 family protein"
                     /protein_id="HIR1112388.1"
                     /translation="MDILVIDGVALPTPNEYKVQLSDLDSSGTGRTEDGILVRERVRG
                     GVAKISVSWAALSTADCARVLNATAPDSMQVAYFFGGTRTAKMYAGDRTADLKAAREG
                     RAVWEVAVNLIEY"
     gene            21051..>22093
                     /locus_tag="ACZPAX_00405"
     CDS             21051..>22093
                     /locus_tag="ACZPAX_00405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1112389.1"
                     /translation="MYPVSERYKTAIRARARTDRVTGTLTLTDGTVLALGVQDFMSGS
                     LTLDNQCVTGEELAFGCVYLGQAAFSLRTSLSRYAFYGAKLVLRYELQLPGGSWEAVP
                     LGVYTVAEAERKALYVSIKAYDNILPLQSRWDGTAIQGNACEMLAQIADGCGLELGQT
                     AEEIAALNPNAALACQLSAADGLTTWRDCVAAIAQLLGGFGTVDRAGRLVIRQFAKTS
                     CVSLGADARSEAGVSDFRCHYAALTVATQSGSYAAGGGQDTGLTMAIADMPLAEKGLP
                     DTRQGITDNLFAELRQLDYTPATVTMPGDPALEPGDRVALPQADGTAPEMLVTHFVWH
                     YHGRQTLKSVGRNP"
BASE COUNT         4211 a         6203 c         7547 g         4132 t
ORIGIN      
        1 gaaattcagg actcttggga ggagggagaa aaagtctctc gtggccgtga acttggtggt
       61 gctgctcatc gtctggcccg gtatctgggg caacgacgac ttggcggtgc tgtctctggc
      121 gcggacgctg cagccaaact cctggcaaca ctttctgacc agcggggcgt ttattctttc
      181 actgatgttc gtgccgatgc ccggcggcgt ggtgttggtg cagaatctgc ttattagcgc
      241 cattgtaggc agctttgcgg ctgcggcgca ggatctgacc gaaaagcggc tgggccatgc
      301 cgtgaagccg gcgtggttcg ccattgtcta cctgccgttt ctgcttccgc cggtcgttat
      361 gcacacgcag cagcccttcc gcaccacatg gtccggctgg acagagctgt tccttgtgtt
      421 tatgctggtg gcaatgtacc tgcggggcac aaagctgaac aagaaggaac tgtgcgccat
      481 cgttgtgctg ggcacgctgg cggtatcctg gcgcagcgag tgcgtctact acctggcggc
      541 catcccggta ttgctgctgg tgatgtgcgc ccggaagctg ctgcgcccca cggcggtggc
      601 ggcggccacc gtgctggtgt ttgccgggta tctggcctgc tcacgctaca caagcaccct
      661 gatgggtgag gcgtggcaat ataagatgat cgcgctgtgc taccagacgg cgtcgctggt
      721 gcaggatgct gacccggtgg cggatgcgga cgagctggcc gccatcgacc gagtgtttga
      781 tgtgacgtac tgccgcgaaa attccaatat ccacggcccg gaaatgcgca cgaatatgac
      841 gcgcgagaca ggcgtgaccg aggaggattg gagcgcctgt cagaaggcat ttttcaagct
      901 ggcgctgaaa taccccaaga gcctgctacg cgagcggatg ggcgtgttct acaacacgct
      961 gcgtcagcgc aagaccggta tgtcgaacca gaagatcgtg ttttcctcgt cgttcctgct
     1021 gtacgatgcc gagccgacaa aagccgacca aaaggagttc tttgcggaca gcgtggcggt
     1081 gcagcccttg aataaagaac tgcgcagggc gtttatcatc aaggtggcaa acggcgacgg
     1141 gataatcgac tggacctggt ggatgctgcc gccctttgtg ctgttgtttg cggcagttat
     1201 cgtgctgctg gtgcagcgga agtggatgct gtttttcgcg gcggggactt tctttgcgcg
     1261 gataccgctg gtattcctga ccgcgccgga tacctacttt ttgtactatc tgacgccgtt
     1321 tatcgccggt tacgccgtgg cggccgccgg ggtgctgtat gcggtgatga aacataaaag
     1381 caaaagagga cgtcttttat gatgaaaaga aagaagcaca ccctgtggct ggaactgggc
     1441 ggtgcggtac tgtggtgcct ggtgacgctg ttcacggata agctgttttt ctggtatgat
     1501 tggcacagct ggcgcttttt tgcctataag gcgctgttta ttctggcggc gttcggggtc
     1561 atccacggcg tcgtgacgct ggtgcagaag gtgcgcgaga aggacagctt tacgttgcga
     1621 tggctgcggt ggacactgcc gtatctggcg gtcacactgc tgcttctgct ggtgctgtgg
     1681 ccgggcatct ggggcaacga cgataaaacg attctggatt acgcgcgcac gttggagccg
     1741 tttgcgtggc atcacttttt gacgtcggtg tggtttatca tttcactgat gtttttgccg
     1801 atggtgggcg gcattgtgct gatccagacg atactcattg cgggaatcgt cgggctgttt
     1861 atcgcagagg cacagacgct ggctgaacag cggctgcaaa agcccctgaa atcgggctgg
     1921 tttgtgttgc tgtacctgcc gttcttgctg ccgccggtcg taatgcacga tatgcagctg
     1981 ttccgcatgg tctactcaaa ctgggtggag ctgctggtgc tgtttttgct ggtgcggtgg
     2041 tacctgcgta acacggcaat caagcgctgg gagcttgtac tttttgcggt tctgggcgcg
     2101 gtggctgcgg catggcgcag cgaggctatt tactatgcgg cggcactgac gatactgttg
     2161 ctgattttaa tgcgtaaggg gctgctgaaa cccatcgcgg caggggcggc cagcgcggtc
     2221 attgtggtgg gtgcgctggc ttgctcccgc tataatgcga gcctgatggg aaatgatttg
     2281 cagtaccaga cgttggcgct gtgcagccag gcggcggcac tggtgcagga tgcggacccg
     2341 gtttccgatg tggaagaatt tgccatgatc gacaacgtgt attccgtcca gaaatgccgg
     2401 gaaaacagca acctgcataa gagcgacctc tttggagcgg tcgtacagcc gaacctgacg
     2461 gaggagggct ggagcgcctg caagaaaggc attgtgaagc tggcgcttaa atatcccaaa
     2521 tcgctgctgc gggagcgcct gggcatgttc cgggcgacga tgcaggcgga ctacgggggt
     2581 tcccgccaga aggatttctt tggatttgct tatgtctgct acgatttgga tggctattac
     2641 ctgaccagca ttgagcgtgc cggaaaaatc gcctaccaga gtccgctggc atttcctatc
     2701 aaccaagacc tgcgcaaggc ggttattggc acgatggtgt atgacaccga gacgccgctg
     2761 ggcaagctga tttcgaccac atggttcatg ctgccgccgc tgctgctttt gtttgtggag
     2821 gcgctggtgc tggcggtacg aaaaaagtgg ttcctgttcc tggtgacagg cacactttgc
     2881 ctacgtgttg tgctggtgtt tttgacggca ccggactcgt tctttatgta ttatctgacg
     2941 ccctacattg caggctatgc aatcgcggcg gcgggcgttg tctatgaagt gatgcgccgg
     3001 aaattgaaag tggaaaggaa tcctgggtga tgaggacact gtatttactg ctggctttgg
     3061 cgctggtgct ggtgggccat accttccgcc tgctgcggtg ggagcagttc atccgcattt
     3121 atgagcggcc gatgcgtggg cggatgctgc gcggtatggc gggcggctat gcgctgaact
     3181 ttgtgctgcc gttccacctg ggcgatgtgt tccgcgcggt gttcaccggg cggaggatga
     3241 agagcggcat cgggttcagc ctggctaccg tcattatgga ccgttttctg gatgtgtggt
     3301 ttgtggcgct ggggttcatc ggtttctggg ctgccgggtt tggcggcgag gtcgtacacc
     3361 gcgcggcgcg gtattacttg gtgttttcgc tggtgctggc cgtggggctg gtgctggtgc
     3421 tgctgctgcg cgactggctg aaaaaaatct gcttggcgtt ctgcggtatc ttcaacgata
     3481 cgctgaaatt ggatggcatg atgttctttt ggagcctcat caacaccttc aaggacctgg
     3541 gccgcgttaa gatcggacgg cttgtggtga acacactggt catgtgggcg gcgtacctgg
     3601 gttcctatgg ggcgctggcg gcggcgctga cggcggcggg tcagccgatg cagctggtgg
     3661 atgtgtttga cctgctgttt ggccgcaacg cggcggattt ggcggcgctg ctgcccggcg
     3721 ggatgctggg cggtgcggcc ccgatggtgc gcggcctgtt ggtgctgtgg tcggtgctgc
     3781 cgctggtcat catgtgggcg gcgacgctgc tgcccgaaac ggtgcgcggc gcagtgaacc
     3841 acgctaccca ggcggccccg gtgggggaaa attacttaaa tctgctgccc caggccgacg
     3901 agcacgaccg cgcggcgttt ttgagcaagt atttcggcct ggaaaacaag gattatgtca
     3961 agcggttctt gcagatgaac cgggatatca cgattctgca ggactattct gcgggatcca
     4021 acgccacgac gatgctctgc atggacaagg aaacaacgtt ctaccgcaaa tatgcctttg
     4081 gcaaggacgg cgacaagctg gctgagcagc tggattggct gcgggcgcag cagagccgcc
     4141 tgccgctgtg tgatatcctg cgcagcggcg aggacgacgg ttattgctgg tatgacatga
     4201 catacagccc gcaggcggtg ggtatgttcc gctacctgca ctccaacccc gtggcgaata
     4261 gccagcgcat tttgcgggaa gtactggaca cgctggaaga aaagctctac aagcccacgg
     4321 cctgcccggc ggatgcggaa aaaattgagc ggtacatcga aacaaaagtg gacggaaacc
     4381 tgaaaaaact gcacgacgac cgcatcttgc gggaattgat gggctatgac acgctgcgca
     4441 tcaacggcac gacctaccgc aacctgaacc agctggggtg gatgttcgac catgagcggc
     4501 tgcgcaagct gtttgcgggg gacccggtgg gcaccatcca cggcgatttg accattgaaa
     4561 acatcatctg ccgcactgac acggacggat ggtacctcat cgacccgaac actggcaact
     4621 tgcacgaaag tccgttcctg gattatggca aactgctgca aagcctgcac ggcagctatg
     4681 agttcatgat gaaaacaccc cgggtgacgg tgcaggatga ccgcattgac ttccaactga
     4741 cgcggtcggc ggcctatgac gcgctgctga cggccatgct ggacgacctg aaagcccgct
     4801 acccgcggga gcaggtggag agcatcctga tgcacgaggt tgtccactgg ctgcggctga
     4861 tgccctacaa gctggcgaag gaccgcaaac gcgcgccgat gttctacgcc gggctggtga
     4921 tggtggccaa cgatgtggcc gattttgtac agggagaggg aaaactatgc tgatctgtgt
     4981 ggacctggac ggcacgctgc tggacaccgt acccgccaac gcggcgtcct accgcgcggc
     5041 gctggaagag ctgggcttta ccgtgaccga tgaatactac gccgagcgct gcaacggcgg
     5101 gcactacacg cggtttttgc cgccgctgat gggcggtaca cccagcaccg ccgatgtaga
     5161 gcgggtacac gaccgcaaaa aggcgctgta cagtgacttt ttggacgtgg tgcggcctaa
     5221 tacggcgttg atggagatac tgcgcacgat gcaggccgcc gggcatgacc tggcctgtgt
     5281 gaccaccgga agcaagcaga acgccaccga ggtgctggag catttcggtg tgcgggaact
     5341 gtttgggctg atcgtgaccg gcgaggacgt ggaaaagcag aagcccgacc ctgaaggata
     5401 ttgcaaggcg atggcacatt tccgcgtgac acctgccgag acgatgattt ttgaggattc
     5461 gggcattggg ctggaagcag ccaaggccag cggagcgacc gtatttaggg tggagcagtt
     5521 ctaagaagca gcattgcagg ggccgggtat gcccgccccg cgcgtggaag gcaagcggga
     5581 ggctgcggaa aggcccggca aagcccctcc gccttcgctc ggtacctccc ctgggagggg
     5641 aggtaccgag cgaaggcgga ggggcgtcga ggatgccccc ctacgacaaa aaacagccct
     5701 tgaggaactt atcctcaagg gctgttgggt tattttgggg aaattacttg ttttcggggc
     5761 ggaccatctc gcggatgacg ttgacgatgt tctcggcgga gaggccatag tcacgcagga
     5821 cctcgttcgc ggggccggag tggccgaact gatcctggac gccgatgcgg cgcacgacgc
     5881 agggcagctt ctcgctgacg acagcgcaga cagcctcgcc caggccgccg atgacgctgt
     5941 gctcctcgac ggtgatgatg cggccgcatt cgcgggcggc ggtcaggatg atctcctcat
     6001 cgatgggctt gatggtgggc atattgatga cgcggacgtt catgccctgg cgcttggcca
     6061 gcacggcggc gcgcagtgcc tcgttggtca tcaggccggt ggaaatgacg gcgatgtcgt
     6121 aaccgtcggt cagcttctcg cccttgccga tctcgaactc gtaggtctcc gggtcatgga
     6181 aaaccggcgt ggccaggcgg gaaaagcgca ggtagacagg gccctggtag ttgtaggcgg
     6241 cgatgacggc ggcgcgggcc tcgacatcgt cggccgggct gatgacgacc attccgggga
     6301 tggagcgcat cagggcgata tcctcgcagc actggtggga ggcgccgtcc tcaccgacag
     6361 acaggccggc gtgggtggcg gcaatcttca cgttcaggtg cgggtagccg atggagttgc
     6421 gaatctgctc aaagccgcgg cccgccacga acatggcgaa gctggaaaca aagggcacat
     6481 agcccatcgt ggacataccg gcggcgacgc ccaccatgtt ctgctcggca atgccgcagt
     6541 caaagtggcg ctcggggtaa gccttgcgga aaatctcggt cttggtggcg gcggccaggt
     6601 cggcgtcaaa aaccaccaga ttgtcagcgc cctgctgggc cagctctacc agcgtattgc
     6661 cgtagctgac gcgggtagca attttcttga tatcagccat tgttgaactc gatctccttt
     6721 tccagcgaag cgtgagcggc gcgcagctca gtcattgcct gtgcatattc ctcgtcgttg
     6781 ggggctttac cgtgccagtc aacggcattc tccatgtagc tgacgcccag acctttggtg
     6841 gtgcgcagca ggaagcaggt gggtttgccg ctgccgtggg acaggtcaaa catcttaaaa
     6901 gcctgctcca aatcggaaaa ggaattgccg ttgcagacga tggtgttgaa gccgaaggca
     6961 tccatcttct tgtccagcgg ctcagagttc atgacgtcgc gggtcgcgcc gtcgatctgc
     7021 aggccgttga catcgaccat gatgcacagg ttatccagct tgtagtggtt ggcaaacatg
     7081 aaagcctccc agcactcgcc ttcctcgatc tcgccgtcac ccagcagggt gtagacgttg
     7141 atgtcggagc cgctctgctt ggcggccagc gccatgccgc aggcggcgga aacgccctgg
     7201 cccaggctgc cggtgctcat atcgacgccg ggcacggtgt tcatgttggg gtggccctgc
     7261 agatagctgt tggaatggcg cagcgtgggc aggtcggcga ccgggaagaa gccgcgctcc
     7321 gccagcgtgg catacaggcc gggggcgcag tggcccttgc tcagcacaaa acgatcccgg
     7381 ttgggatcct ggggctgttc ggggtcaatg cgcatttcac ggaaatacag gtaagtaagg
     7441 acgtccgccg cggacaggct gccgcccgga tgaccgcacc cggcaccgtg ggtggcttcg
     7501 atcacaccca tgcgcacgcg ggtcgcagtc agtttcaact gcagcgtttc cagttttgtc
     7561 atagctaagc tcctccggtc actaatgctg aaattgggca gtattgagag atactgctac
     7621 acaatatact actatatatt atacacgttt cgtaaaatct cgcaacattt ttttacaaat
     7681 ttcatggaaa atcagcccct ttgcatccaa agtgcaaaaa gtgagggaca gcaaagcctt
     7741 ttgttatgcg gcctttgtgc gttgtgccaa aaaatggggc gaatcgtaca taaactacgg
     7801 cgaaaaatac cttgcgcccg gccggatttt atggcataat tggggtacag ccgcggtgcg
     7861 ggacgccatg atgtggcaca tccgcccggc ccacactact atatctggga gggaaagagc
     7921 gtgcccaatg gaatttatat ccagactgag taccacggca aacttatccg caaaatcgtc
     7981 tgtaatgggg acgagcgctg gtttatcggc tccaactgcg ccgtgacgtt tctgtctatg
     8041 acagactgca tggctgccat tgaccgtctg taagaaattc tgaatgcaag gccgcacgcc
     8101 gtccccctgt gggatgggtg cggtcttttt tgctattgag gtgtgcttat gaaaaaacgg
     8161 aattggcggc tgatgctgcg cagggtgctg aacctggtgt tcagccgcct ggtcatcacg
     8221 gtaacactgg tgctgctgca agtgttctgg ctgttctcgg tgttccactg gctcgacgcc
     8281 tattcgactt ggctcaacgg cgtggggctg gcgctcagcg tcattatgtg cctggcgctt
     8341 atccggcagg attccaccgc gccggagttt aaaatcagct ggatgatcat ttttatgctt
     8401 atgccggtac agggcgggct gctctacctg ctgtggggca acaagcgccc ggcgctgggc
     8461 ctgcgccacc ggctggaacg cgccgaaacc gccatggccc acacccggcg cgaggaccct
     8521 gccgctgtgg ctgccctgcg gaagcagaac ccccgcgcgg ccgagacagc gcgctatctg
     8581 cacgactacg cccccgcgcc gttgtgcggg gacacggcgg tgaaatacta ccccgatggc
     8641 cagagtatgt ttgccgatat gctgcccgcg ctgcaaagtg ccgagcacag catctatgtg
     8701 gagagcttta tcatcggcat gggcgagatg tgggggcaaa ttcacgagat cctgcgccag
     8761 aaagccgccg cagggctgga cgtgcgggtc atctacgacg atgcgggctg cctgagcctg
     8821 ctgccccaca actacgctga aatgatgcgg gccgacggca tccgggcgtt cagcttcaac
     8881 cgctgcgtgc cgctgctgaa tttggtcatg aacaaccgcg accaccgaaa gatcatggtc
     8941 attgacggca aaatcggctt taccggcggc gtgaacctgg cggacgagta tatcaacaaa
     9001 ttggagcggt tcggctactg gaaggacagc ggcgtgcggc tgaaaggccc cggtgcgact
     9061 gcgctggcga acatcttttt gacgttctgg aaagcgcagt accccgacga ggaaatcgac
     9121 atggaccgcg acctgccgct ggccgcgccg gtcaaaacgg actgcctggt gcagcctttc
     9181 gctgacagcc cggtggaccg cgaggccgtg gcgaaaaacg tctacctgga acttatcaac
     9241 caggcgcagc ggcggctgta catctgcacg ccgtacctga tactggataa cgacctgctg
     9301 acctgcctgc ggctggcagc caagcgcggc gtggatgtgc gcatctacac gcccggcgtg
     9361 cccgacaagc cgacaattta tcagctgacg cggagttatt tcccgcatct tttgcgggcg
     9421 ggggtaaaga tttacagcta cacgccgggc tttttgcacg ccaaaacctg gctggtggac
     9481 gaccgcattg cggcagtggg taccgtgaac ctggattatc gcagtttgta tttacacttt
     9541 gagaacagtg tgctgatcta cggcggggct gtgctggaag atgtgcggaa agatttggcg
     9601 gaaattgagc gggagagcaa agaattgacg ctgggggatt gccgcaccgg gtttgtgggg
     9661 acaatgtata gtgcggtgct gaggttggtg gcaccgttgt gctgaggaag aacgctttgc
     9721 aggggcgaac aatgttcgcc cctgcaattt tttacgtgta tcggttttct acggcttcta
     9781 taaattttac ggggtcgatc atgcgggcac cgtcggcggt tggggctgcg gtggagacaa
     9841 gcagggcaag catttcctca ttggtgaggg cgggggcgac ctgccagccc agcgccatta
     9901 ctccgacgca gtaggggatg ccccagctca agccgccctg cacgtcatat cggtagccgc
     9961 actgctcgga aatgtattcc tcggctacgg tgcggtaggc gcagggcacg cccacatagt
    10021 ttggattttt cctggcgagg ggggtgataa ggaaagcccc gggctggccc atccggcagg
    10081 ctgcgggggt atcccgctgg gctgggtcga aggcggcaag acccggcacg agacgcgcag
    10141 agcctgcgct tgcgccttgc acggtcagta ccagaaggcc cgcgtcctcg gcgtcggaaa
    10201 gagccgcctg ccagaggccg gcgttttcaa aaaaattggc attccccgga ttggcagaca
    10261 ccgacacggc acgaattttt ttgctttccg gcagggtatc atttaaggca aggatgtaat
    10321 gaagggcgtc ggcgtagggg cggctgtcgg ctgtgccggg cgtgaccgcg taatagatat
    10381 gtgcgcccgg cgcaacgccg atcgtttttc ccgccagaat gctggccacg gcggggccgt
    10441 gcatggtgcc ctcgtccgtg tagccgcctg cgtcgtaata gtcaacgatg gcgtcggaaa
    10501 tttcgtgatg gtcggtcagc agtggctggt cgatgatggc aacattgacg ccctgcccgg
    10561 tgatgctgcg ggcctgcagc gaccgcacac cgaggccggg gtctctgccc gcttccagta
    10621 atgcggcggt ttcctcctcg gccccggcaa ggacggtgcg gtcattgaaa gagtaggtgt
    10681 tgaggctgag gcgatcgtat tcgcccggtt ggtaggtctg cttttgatgc gcggcgtccc
    10741 aagacagctc cggcaggtag gataggtcgc tgtacggggc cgggacggag aagggcaccg
    10801 tggtgggtgt cgctgcgggt gcttcggtcg gcgcaggcga ggccgcgggg acggcagttt
    10861 gcgcggccgt gcaggcagtc agcgccagca gacaggcggt gaggaggaaa aagcgtttca
    10921 tggagagatc cctcgctata aaaaggtcag gagccgctgg ggccgcggga actgacgctt
    10981 gcaggcgggg cggcggtgta aaaggcggtg ccgtttacgg tgctctgtgt catgccgacg
    11041 aggtcgagca gctggttttc cgtgtagggg gcagcgtcca gcttagggga cgtgcagcgg
    11101 tagacgccgt cggagaccga gaggatacca cgctttgcca ggcgggccag cgatttttca
    11161 agcatctgtg agtcgcaggg atagcctttt tcggtgaggg ccgccgcgag ctgagcggcg
    11221 gtgcgcggct tatccctgag caggagggcc agggcgagct gctgcccccg gaggcggtat
    11281 gggagcagac cgcagcgaag ccaaatacct tttgtacact tcatgttgga acactccttt
    11341 ttctcgtagt atcgtcatcg tagcatggat aggagcgggt gtcaagcgcg ggaaatgaaa
    11401 aatggagtgg aagtcctttt tttggtacag ccaaaaagaa aatgcggaaa agctattgac
    11461 aacggatggt tgttgatata ggatggagat aaccaacaaa gcctgacagg gaagcgaaac
    11521 gtgcaaataa gggaggacgg acaatgacat accaagaaaa gatgaagtgg ctggggcggt
    11581 atcaggcggc gctgagctac cagcatatgc tggaggatga gatcgaggtc ctgcgcagtg
    11641 atgccgagcg tgtgaccacc tgcatgagcg ggatgcccgg gcgcggcggg ccgaacgtcg
    11701 accggctgcc gcgggcggtg gaacgcatcg aaaaggcgca gaaaaagctg gcgcagcagc
    11761 tggacagctg cctggaaacg cggatggaag tcatgcacag catcatggcc ttgagcgata
    11821 ccgccgagca ggaagtactg cgccgccgct atgtgatggg ccagagctac accgatattg
    11881 ccaatgcgat gggcgtagtg cagcgccggg tctaccagct gcacaaggcg ggtgtggaga
    11941 aggtggagct gcggagctga aaacatttca gtaaatttcg caaaggtttc gcggaatttc
    12001 attgcatttc acaactaaca gtggtaacat ggtagcatcg aaactcgtga gaggaaccaa
    12061 atcctcccgc gagttttttt atacccaaaa ggagggatgc cgttgtgcga aatcaatgag
    12121 gagcacgtcc tggacgaact ggcggagatc gcctttgcca cccccggcga ggagggcggc
    12181 ctgccggtca aggtggccga caagctgcgg gcgctggaga tgctgtacaa gcatctgggt
    12241 ctgggcgacg gcgcgaagga tgagggggtg gtcattgtgg atgagtaaag agagaagaaa
    12301 gaaaagagag ggggcgcggg ggtgaagatc cggctgaaag agaagatacc ggcggtgttc
    12361 tggcaggtgc atcaggcgat acggcgcggc gaggtgacgg aggtcgtggc caagggtggg
    12421 cgcgggtcgg gcaagtcaag ctatctttcg atggagctgg tatggcagct gctgcgccac
    12481 ccggactgcc acgccgtggt gctgcgcaaa gtgggcggca cactgcgcaa cagcgtgtac
    12541 aatcagatcg tatgggccat cggtgagctg ggctgcgcgg ggtatttccg ctgtacggtc
    12601 agcccgatgg agtgtaccta cctgcccacc gggcagaaga ttttgttctt cgggttggat
    12661 gacccgggca agctcaaaag tctcaagctg ccgtttggcg ctgtcgggct ttgttggttt
    12721 gaggagcttg accagttcga cggtcccgag gaagtgcgca acgtcgagca gactgtcctg
    12781 cgcggcggca gttggaccct gacgctgaaa agcttcaatc cgcctgccat ggcccggaac
    12841 tgggccaacc gctacgcgct ggagcagcgc cccggcaagc tggtccacca ctccacctac
    12901 cgcgatctgc cgcaggaaat gctgggcgca cggttctggg ccgacgccga acacctgcaa
    12961 aggacgaacc cggcggccta tcgccacgag tacggcggcg aggtcgtggg cagcggcgcg
    13021 gcggtgtttg agaacttgca gctgcgggcc gtgacggatg ccgagatcga gcggttcgac
    13081 cgggtgtact acggcgtgga ctggggctgg taccccgacc cctgggccta caatgcggtg
    13141 tattatgacg ccgcgcggcg cacgctggtg atttttgacg aactgacgcg gaaccgcacg
    13201 cccaaccgcg agacggccca gctgctggtg gagcgcggcc tgaccgaccc ggacagcggc
    13261 acactgacgg cggacgccgc cgagccgaaa tcctgcgccg actaccgcgc cgccggactg
    13321 ccctgccgcg cggcccagaa aggccccggc agtgtgcggc aaagcatgaa atggctgcaa
    13381 gggctggcgg ctatcgtcat tgacccggtg cgctgcccgg ccacggcggc ggagttcagc
    13441 gagtacgagt acgaccgcga cccccgcacc ggcgaggtgc tggcgggcta cccggatgtg
    13501 aacaaccacc acattgacgc ggtgcggtac gcagtggaag gggtgtggag gcggagaggg
    13561 gcgtgaaaag gcgcagagaa tgtaggggcc gcacatgtgc ggcccgcaag tgtaaggcag
    13621 acgaccgctt atgtaaaagc gtagggccgg gcatgcccgg cccctacgaa gcagggaagg
    13681 ggaggcaaag agatttgaga ggagaggatg aattgcaatc gtatctggaa caagcgtttg
    13741 ggaaaagtga cgttacatcg gtgaagatgc gggcggcact gcgggagtgg ctggacttgt
    13801 actacggcac gccccgcgca ggcgaggacg ctgcgccccg gctggcggcg ctgatcgtgg
    13861 gcaaattatg ccgcaccgtt tttgccgagt acgagacccg gctgcccgaa aacgccgggc
    13921 agcctatgcg gtgcagcctg gctgcactga acgccatcgc aaagcccgcc atgcagtacg
    13981 cactggtggg cggtgaatgc ctgctgaaac ctgtaccgaa ggacggcgca ttcgactttg
    14041 tacccatccg gcgcgactgc tatgtgccgc tggcgcggga tgcccacggg cgcattctgg
    14101 ccgtgggtac gatggagcgc cacagctgcg acggccgcca atatgcgctt ctggaacgcc
    14161 gcacggccgg ggctgagggt ctgaccatcg agacgcggct gtttgagctg aacggtcagg
    14221 cgctgggccg ctgcgtgccg ctggccacac tgcccgcctg tgccgacctg gtcccccagc
    14281 tgatgctgcc cggcgtgcag ggcgtggggc tggctgtgct gcgcaccccg ctgatgaact
    14341 gcgtggacgg cagcgccgac gcggtgagta tctacgcccc ggcggcgggc ctgctgcacg
    14401 ccctggcccg ctgcgaggaa cagctgaaca ccgaattcgc caacggcgcg tcccgggtgt
    14461 ttgcgtcgga ggacctgctg cggcccgatg cccaggggcg gcgcgcgctg cgagatgatc
    14521 tgttcatcgg cctgccggac gacccggcca atgtgggcgt gaccgtgtac agccccgccc
    14581 tgcgggagca aagctacctg gcccgcaagc aggatctttt gcgcgggtgt gagagtctgc
    14641 tggggctgcg gcgcggtatt ttgagcgaga ccgaagccaa cgccgacccg cgcacggcca
    14701 ccgaaattgc cgcaacgtcg gtggactacg atttgaccat ccgcgatttg cagggcgcgt
    14761 ggggcgctgc ggtgcaggac gccatggtgc tgtgcggcac gctgggagcc ctgtatgggc
    14821 tggaatatca taccgacgcc gttgtcccgg ctatcgactg gggcgacggc gttttgtatg
    14881 accgggcgcg catctgggcc gaacagcggg agatggtgga cgccggactt ttgcgccccg
    14941 agctggcgct ggcctggtat tttgacctgc cctgcggcag cgaggcagag ctgaaggagg
    15001 tgagggagaa gtttatgccg aagtgagaga agagagaaga aagaagaggg aggtgagaca
    15061 agatggacga gcagagcagg cagacccccg cgccctatgc ggcaggcacc ggcagtgtgc
    15121 cggtagcccc cgaccgtgcc gcgtttgacc ggatgggata ccgcgagcgc ttggcactga
    15181 agcgggaaaa cccggaggtg tatcaggaat tgaagaagta gggggccatc cccctgccgc
    15241 ggagggggat ggccccccct acaatgcgac cgaaaatgag gcagctatca aaaaggtgag
    15301 gaccgggtat gcccggccca taccatgtga ttatcaaaaa ggagaaaatt ttatgtctga
    15361 ttttattacc aaactgtccg atatgatcga cccggaagtc atgggggata tggtgtccgc
    15421 gcgcatcccg aagaagctgc gcgtcgcacc ctttgctaaa attgacgata cattggcggg
    15481 cgtgcccggc gataccatca ccgtgcccgc ctacacctac atcggcgacg ctgccgacgt
    15541 ggccgagggc ggcgaggtcg ccatcgagaa gatgacgacc tccacccgca aggctaccat
    15601 caagaaggcc atgaagggca tcggtctgac cgacgaggcg gtgctgtccg gctatggcaa
    15661 cccggtgggc gaggccaata cccagctggc gctggccatc gctgcgaaaa tcgacagcga
    15721 ctgcatggac gctttgcaga ccgcgagcct tgtctacgac ggcagcaagg agaatatcag
    15781 ctacaacgcc attgtggacg ccgtggatct gtttgaggag gagatgggct gttccgacaa
    15841 ggtgctgttc atccacccca agcaggtgac ccagctgcgc aagaatcccg actttttgag
    15901 cgccgacaag tacaccccgg gcgtcagcct gaccggcgag atcggcatga ttgcaggctg
    15961 ccgcctggtg cccagcaaga aggtcccgct caccggcggc gtctacgcct gccccatcgt
    16021 caagctggag gccgaccccg aggtcgatga cgagattccg gccctgacca tctaccgcaa
    16081 gcgtgaagtc aacatcgaga ccgagcgcaa gcccaagacc cgcaccaccg agatcacggc
    16141 tgatgaattc tacgtcgctg tgctctccaa cgaggccaag gtggtgctgg caaagttcaa
    16201 ggcgtaaggg ggcgggagcg tgccggatta cacctattac accgagaaat acctgggcga
    16261 ggatatccct gaggctgcct tcccgcggtt tgcccgccgt gcggcggaag aactggcccg
    16321 gatgcagaac atctacccca tggccccgtg caagggcatc gagccggaca cggcctacgc
    16381 catggcggtg tgcagcatcg cggatgcaat gtatgagttc gcgcaggagg acgaggcccg
    16441 gggcctggcg aaggtcagcg tgggcagcgt gagcgagacc tacaccgcac cgccccagct
    16501 gtgcgccgcc acgctggccc tgcgggcggg ccactaccgt cacgaagcgg gctattacta
    16561 cgagatcgga cggtggctgc cccatgcgta aaaaccggct ctgcggcgag accgtgacgc
    16621 tctaccaccc ggatctggcg gcgcagaccg tttgccgccg ggtgctgcgg ggcgtgagct
    16681 ggcagcaggg ccggcgcgaa aagcccgatg ctgccgggac aaagcaaggc acggcgctgc
    16741 tgctggtcat ccccgaagcg gcggggcagt acggcgcgga ctacacgctg gaccccggcg
    16801 accgggtgct tttgggcgag ggaccgggcg tggcctgggc cgattggccc ggctttgtgc
    16861 ccgctgctgt ggcggggctg gcggttgtgg tctatgtgct gccgctgcgc ctgcgcgggc
    16921 gggtgcatca tgtggaggcc ggggcctggt ggaacgccag cggcaccggg gcccacagct
    16981 tgacgaggtg aaaaaacctt tggcttctcc ctcgtgggag aagctgccgc ccgcaggcgg
    17041 ctgatgaggg gcgaaccggc cgctgctgca cgggaaaggg acgccgggtg gcaggctgcc
    17101 cctcatccgg ccctgcgggg ccacctttcc ccaaaggggg aaggcttaga gataggagga
    17161 ctaccatgat gcaaaaactc tacgcatttc tggcccgggc accggccctg gccggggtca
    17221 cgctggcggt gggggatgca ggtcccgcgc cgggcacggc ggggctgtgg tgcaagggcg
    17281 tcacggtgct ggaacagagg gaaaacctgc tgggcaccgt gcgccagcgc tgccgcgccg
    17341 agttcacgct gcgcctctgc ctgccgctgc cgcccgggga cagtgccacc gctgccgaaa
    17401 acgccgcccg cctgctggct ttgcagacct gggtggccgc cgagtgtgcg gcgggccgtg
    17461 ccccggtctt tggcaatgcc gacccggccc gggagaccct gcgggccgag cagggcaaac
    17521 tggaacgcgc cgacgcaggc ggcaccgccg tgtacagtct gcgcatccgc gccgaataca
    17581 cacaacttta tacggaggaa accccatgaa aattgaacga aaatacatgg cccactacct
    17641 gaacgccgcc tttggcaagg gcgaggccga ctacacccgc ctggggcagg acctggaaga
    17701 gtacgccccc gaactgactg ccaacgttga gaaaaagtcc aacatcctgg gccagacgtc
    17761 ggtggtcatc gacggctacc agaagcaggg tgaggtcagc ccctactacg ccgaggaggg
    17821 cgacccgctg tttgaaaaat tgcaggggat catcgacggc gacctggtac tggacgacct
    17881 gaaaaccgac atcgtcgagg tcaagctctg gagcaaggac acggccgggg cctaccccgc
    17941 cgtgcgcgag gaatgctaca tcgagatcgt cagctacggc ggcgatacca ccggctacca
    18001 gatccccttc aacatccatt acaccggcgt caagaccaag ggtacgtttg acccggcaag
    18061 caagaaattt acggaggcat gatgaatgaa aaagctgaac attgacaccg gcgtggagga
    18121 attttccgtc aatgggcagg gcgtactgcg gttcaacccg gctgacccga acctctacca
    18181 ccgctttttt gcggcgggcc ggacgctgaa cgactacgat gccgccctga cgaaggcctt
    18241 gcagactgtc gagggcgacg agcaggcctg cgccgcggcg gggctgcgcc tgctgaacga
    18301 gtacgacggc aaaatcaagg cgctgctgac cggcatcttt ggccccgaga acgactttga
    18361 cgcgattctg ggcggtgtga acctggcggg cgtgggggcc aatggcaagc gcgtggtgca
    18421 aaacctgctg gaagccctga cgccgattct gcgtgagggg gctgagcgcc gcctgaaagc
    18481 cgccgccgac actgcagaac gtgaggccgc tgccgcccgc gcggcccggg gcgcggtatg
    18541 acaggcccct ggacgctgcc gacgcaggcc gcgctgggcg gcggggcata tgtcatccgc
    18601 accgactacc gggacatttt ggaattgctg cgctggctgg gcggccaggc cgacccggcg
    18661 ctggatcagg ccgagcggtg gtacgtggcg atggggctgt tctacccgga atttgacgcc
    18721 atgacgcagg atgtttggcc ggaggcaacg cgctttttag cggagttcct ggcggcgggc
    18781 cgcagagagg aaccccgccc cggccccgcg ctgatggact ggcagcagga cgcgccgctg
    18841 attgcggccg gcattggccg ggcggcgggg caggatgtgc gcacgctgcc ctacctgcac
    18901 tggtggagct tccttgcgct gtttgacgcc atcggcgagg ggagctttgc cacggtggtg
    18961 gccatccggg acaagctgcg gcgcggcagg cggctggaaa actgggagct ggatttttac
    19021 cgcacccacc gcaccctggt ggaattgcgc ccggcggcca ccgcagagga gcaggctgag
    19081 aagcagcggc tgttggcaat gttggggtga aaggagtgaa caaaacgaca aacataattt
    19141 ttgacgaact atcgacgctg cggctgccgt ccggcagctt gcagacggcg ctggataagg
    19201 tggccgcggc gctgggcagc gtgggggatg ccgggcagca ggcgcggcag gtagtggacg
    19261 agctgcgggc atccttgcag gcagccgccg tgcagggcgt aaagtccgcc cgcagccttg
    19321 ccaaatttga tgagatcaac cgtctgtccg ccccggcaac ggaaaagacc gccgcttccg
    19381 ccaaagaaaa acaggcggcc caagccgccg ccaaagcggc aaaatccacc gccggcacca
    19441 cggcgggcag cacccgccgc agcggtaagg ctgggacgga tgcagccgag ttttccgccg
    19501 tgtggcagca ggtactcacc cagctgcgcg gcatctgggc ggatttttgg gtctacctgc
    19561 aaggcttttt cgcgccgttc gtcgcggcct ggcagacggt gtgggcgggc cttggcgcgg
    19621 cggtgagcag cgtttgggag ccgctctggg ccgagctttc caccgtggcc acgcctgcgg
    19681 tggagctgct ggacacagtc tggcaggggc tttggcaggg agtgcaaaac gcctgggccg
    19741 tttacggtca gccgattttg gaggggctgg cccaggggtg gcagaacgtg gtgagcatcc
    19801 tctcggcgct gtggggcggc gtggtgcagc ctgtattttt tcagctgttt gacctgctgg
    19861 cggcgctctg gtcggcgcat ctgcagccgc tttggaacga gctgaccgct tgcttgggtg
    19921 cggtggcagc gctgctgctg acggtctgga acacgatcct ggccccgctg gtgcagtggc
    19981 ttattgccac gctggccccg gtggccgtgc aggtgttcac ggcggtgggc attgccgtga
    20041 ccggcgcggc gggtatcatt gccgatgcca tcacccttgc gctggcggtg ctgcgcggcc
    20101 tggccgactt tttgaccgct gtgctgcggg gcgagtggga cgctgcctgg aacgccatgg
    20161 ccgcgaccgt ctcgaccgtg tggggacgca tcgtctccat cgtgcagacg gcggtctcca
    20221 cggtgctggg cgtcgtgcgc agcatggtca gcgccattgc ggcggccatc aatgggctgc
    20281 tgtcggccat cgggcgcgcc aagtcgatgg cgggcagtgt gctcagcggt gcggggcagc
    20341 tgcttacggc gcaggcggca ttgcccgggc tggggtatgc ggcgtcggtg cccatcccgg
    20401 cgctggcgca gggcgcagtc atcccgccca accgccgctt tctcgccatg ctgggcgacc
    20461 agaccggcgg cacgaacgtc gaagcgccgc tgtccaccat caagcaggcc gtggcggaaa
    20521 cgctggctgg gtggcagggc ggcgacggcc agcccatcaa catctatatt ggcgaggagc
    20581 tgctggacag cgtcatcgcc aacagccaga accgccgtgc cctgcgcagc ggtgggaggt
    20641 aactcatgga tattctggtc attgacggcg tggcgctgcc cacgcccaac gagtacaagg
    20701 tgcagctctc cgacctggac agcagcggca ccggccgtac agaagatgga attttggtgc
    20761 gcgagcgggt gcgcggcggc gtggcgaaaa tcagcgtcag ctgggccgca ttgagcacgg
    20821 cggactgcgc cagggtgctg aacgccaccg ccccggacag tatgcaggtg gcctactttt
    20881 ttggcgggac gcgcaccgcc aagatgtacg cgggcgaccg caccgccgac ctgaaggccg
    20941 cccgtgaggg ccgggccgtg tgggaggtgg cggtgaatct gatcgaatac tgattttgca
    21001 ggggcggatg caagcatccg cccctgcaac caaacgggag gtgaaccatc atgtaccctg
    21061 tatcggaacg atacaaaacc gccatccggg cgcgggcgcg gacggaccgg gtgaccggca
    21121 cgctgaccct gaccgacggc acggtgctgg cgctgggcgt gcaggacttc atgagcggat
    21181 cgctcacgct ggataaccag tgcgtcaccg gggaggaact ggccttcggc tgcgtctacc
    21241 tggggcaggc ggcgttcagc ctgcgcacga gtttgagccg ttacgccttt tacggtgcca
    21301 agcttgtgct gcggtacgaa ttgcagctgc ccggcggcag ctgggaggcc gtccctctgg
    21361 gcgtctacac ggtggccgag gccgaacgca aagcgctgta tgtgagcatc aaggcgtatg
    21421 acaacatcct gccgctgcaa agccgctggg acggcacggc catccagggc aatgcctgtg
    21481 agatgctggc gcagatcgcc gacggctgcg ggctggaact gggccagacg gcggaggaaa
    21541 tcgccgcgct gaaccccaac gctgccctgg cctgccagtt gagcgccgcc gacggcctga
    21601 cgacctggcg ggactgcgtg gcggccatcg cccagctgtt gggcggcttt ggcacggtgg
    21661 accgcgccgg acggctcgtt atccggcagt ttgccaagac aagctgcgtt tcgctggggg
    21721 ccgatgcccg cagtgaggcg ggcgtgtcgg acttccgctg ccactatgcg gcgctgaccg
    21781 tggcaacgca gagcggcagt tacgccgcag gcggcgggca ggacaccggc ctgacgatgg
    21841 cgattgccga tatgccgctg gccgagaagg gcctgcccga cacgcggcag ggcatcaccg
    21901 acaacctgtt tgccgagctg cggcagctgg actacacccc ggccaccgtg acgatgcccg
    21961 gcgacccggc gctggaaccg ggcgaccggg tggcgctgcc ccaggctgac ggtacggccc
    22021 ccgaaatgct ggtgacgcat tttgtctggc actaccatgg ccgccagacg ctgaaaagcg
    22081 tggggcgcaa ccc
//
LOCUS       DBMDSQ010000005        21021 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_844_length_21021_cov_1.989627, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000005 DBMDSQ010000000
VERSION     DBMDSQ010000005.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 21021)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 21021)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21021
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_844_length_21021_cov_1.989627"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(202..1524)
                     /locus_tag="ACZPAX_00410"
     CDS             complement(202..1524)
                     /locus_tag="ACZPAX_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR1112390.1"
                     /translation="MKKLMGILLATSMVFSLAACGSTADTAASTATASAATAEGSSDN
                     QAPDVKVGAIILGDETEGYSAAHINGIKEAAAELGMSDDQIVWKYKIAEGGVTADAAE
                     DLVGQGCNLIISNSYGHQTYMEEEAEKYPDINFVAMTGDFAAISGLDNFYNAFTAVYQ
                     SRYVSGVVAGMKVKDLVESGTLTPETQPDSFTADGKVKIGYVGAYNYAEVVSGYTAFF
                     LGVQSVYPDVQMEVMYTNSWFDIDKEGAAAEALIANGAVIIGQHADSTGAPAATQKLK
                     DSGKICYSIGYNIDMLATAPTAALTSATNVWKVYYKELFEGAQAGSIPQDWCKGYEDG
                     AVAITDLGPEVADGTAEKVAEVEAALKDGSLHVFDTSKFTVDGAEVTTAPIDLTYYDF
                     STGSPVAVYQGETKEAISDGYFHEGELRAAPTFSLRIDGIIEDADPVA"
     gene            complement(1849..3180)
                     /locus_tag="ACZPAX_00415"
     CDS             complement(1849..3180)
                     /locus_tag="ACZPAX_00415"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1112391.1"
                     /translation="MSKFIRELGKIAVPVTLQSMLQASFSIVDQIMIGQLGAVSIAAV
                     GLGGNYSMIFNVVSGSVGTVAGILIAQFLGAGDKKEAWSSLTLSTVISLCLAGVFLGV
                     SLGATAQILGLYTKDAAIIAEGCVYFKIIAFSFAPMGLSTVLSAWLRCRGHANIPFAA
                     SIGAVLSNVVLNYVLIFGKLGLPACGVRGAAIATLVSQLVNLALIATGFLHCLRQDGE
                     RVQPSLHFTKITRRDYLGMITPILVSQFLWSLGQNVESAVYGHLGTQNLAAYTLTCPI
                     QSLIVGALSGLSAAAGVLIGKRLGRKEYDAAYGDAKKIMVTGLVGAAVVSSLLVGLAG
                     QYVGLYRVEGEVRTLGHMLLVVFALYAPIKVENMILGGGILKSGGNTRMVMIIDIAGT
                     WGIGIPLCFFAAYVLHWGIVGVYTLLTAEELFRLVVSLVVFRKRKWMVSLA"
     gene            complement(3165..4190)
                     /locus_tag="ACZPAX_00420"
     CDS             complement(3165..4190)
                     /locus_tag="ACZPAX_00420"
                     /inference="COORDINATES: protein motif:HMM:NF012742.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR1112392.1"
                     /translation="MQENRIRIVDIAERLGLSTATVSNVIHGKTSKMSPRTAQRVWQE
                     LEAVGYIPDMAGVWLAQKSSRIIGVVVDDSPKYEGKVLEDGFVTASLNALSREANEKG
                     FFLMVKTTRTLDEVPAFASMWRMEGLILMGFCEADYAALRQAIRTPIVVYDGYMQGCP
                     GVVNLVIDHADGGRQAGQYLKAAGHTRALCLADNNICMDKERMDGFAAAFGPDRVTRW
                     QVPVQARPRQQFYEENFAALRTSGITAIFAVSDFYALECMRFLQAKGLQVPGDMAVVG
                     FDDSEASRTGYPSLTTVRQDAALRAGIAVASIEAMGRGQSCAEKIVLPVTLLPRESTG
                     GVSCQNL"
     gene            complement(4332..4922)
                     /locus_tag="ACZPAX_00425"
     CDS             complement(4332..4922)
                     /locus_tag="ACZPAX_00425"
                     /inference="COORDINATES: protein motif:HMM:TIGR04518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HIR1112393.1"
                     /translation="MCTVSEQTLSVLTPDYWRTARRELNNPRVLAFSGLVCAMAIVLE
                     SLPIYLMGPSLKIYFSFLAVSLGCSCYGPVVGMMAGAIIDSVGFLLSGYGEPYFPGYL
                     VTAVLSGLIYGVMLYRRKPTLPRIIVTRLIINYGSNVLLGSVWKAMLYGKGYLYYLTS
                     GAVKNTIMLPIEVFLTWVVLNAAAKQGLDRKFIHKR"
     regulatory      complement(5018..5131)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF01831"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="THF riboswitches regulate genes encoding folate
                     transporters and folate metabolism; They are most commonly
                     found in Clostridiales and Lactobacillales; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="tetrahydrofolate"
                     /db_xref="RFAM:RF01831"
     gene            complement(5206..6786)
                     /gene="asnB"
                     /locus_tag="ACZPAX_00430"
     CDS             complement(5206..6786)
                     /gene="asnB"
                     /locus_tag="ACZPAX_00430"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012102652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0070981 - L-asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HIR1112394.1"
                     /translation="MCCIMGYAGHDLPKETFTEYLLRTTSRGPDDQRVAETEFGLLGF
                     GRLAIMGLTPEGMQPFFRGADCVACNGELYGFRPVKAELEAEGYTFESDSDCEIILPL
                     YYKYGLDMFKHLDAEFAMILYDSRKKLLIAARDPIGIRPLFYGYSESGRIAFASEAKN
                     LVGLCKKIYPFPIGSYYCNGQFVRYCDIADTPAYSQDELPDVLTNIREKLVAGVDKRL
                     DADTPVGFLLSGGLDSSLVCAIAAKKLGKPIRTFAIGMSEDAIDLKYAKQVADYLHAD
                     HTEVIINKDIVLDALESVIAMLGTWDITTIRASVGMYLVCKYIHEHTDIRVLLTGEIS
                     DELFGYKYTDYAPSAAAFQAESQKRIRELYMYDVLRADRSISVNSLEARVPFGDLDFV
                     KYVMAIAPEKKLNTYGKGKYLLRKAFEGDWLPESILWREKAAFSDAVGHSMVDDLKEY
                     AETIYTDREFAEKCGKYTYGRPFTKESLLYREIFEKYYPGQAEMIVDYWMRNKAWPHC
                     DVNDPSARVLANYGASGQ"
     misc_binding    complement(6856..7118)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            7549..8784
                     /locus_tag="ACZPAX_00435"
     CDS             7549..8784
                     /locus_tag="ACZPAX_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HIR1112395.1"
                     /translation="MAKNAIVGQSGGPTSVINASLAGVYESCKRRGAGKVYGMLHGVA
                     GLLERRTCVLDDKLKTALDIELLKRTPSSYLGSCRYKLPDWHTAEGEAVYIQLFEILK
                     ELDIGYFFYIGGNDSMDTIGKLADYGAHIGSGIRFMGVPKTIDNDLMVTDHTPGYGSA
                     AKYIGVVMKEIIRDATVYGTKYVTIVEIMGRNAGWLTAAAALAKAEDCEGVDMICLPE
                     VPFNVDHFVEKVERMQKEKPSIVIAVSEGVKLEDGRYVCELADDVHAVDAFGHKALTG
                     TARFLANTVARRLDTKTRCIELSTLQRCAGHLTSRTDITEAYQVGGAAAKAAFEGHTG
                     EMVALDRISNAPYQCGTSLHPISEVANLEKKVPLEWVNADHTAMTDEFLAYALPLVQA
                     ELTPIYVEGLPHHIYLPEA"
     gene            complement(8945..9421)
                     /locus_tag="ACZPAX_00440"
     CDS             complement(8945..9421)
                     /locus_tag="ACZPAX_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005354158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease domain-containing protein"
                     /protein_id="HIR1112396.1"
                     /translation="MGSIKKAPLSKGAVADKATEGFQSISLARNRKESLLMTGKHNKE
                     LTPIARRLRKDMTKEERHLWYDFLRGYPIRFLRQKVVGKYVVDFYCAAANLVVELDGS
                     QHFEDAGRVCDAARTKFLQKYGLRVLRFANNDVMKNFEGVCTAIDKAVQERMELLP"
     gene            complement(9408..12353)
                     /locus_tag="ACZPAX_00445"
     CDS             complement(9408..12353)
                     /locus_tag="ACZPAX_00445"
                     /inference="COORDINATES: protein motif:HMM:NF021134.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HIR1112397.1"
                     /translation="MTKQREKQNITLWLLLGCGLALRLVLALVTEGYPYDMSCFVAWG
                     DKLAAEGPAAFYSEGYFADYPPGYLWVLGLVGAIRAALHIAYESKWTYFLLTLVPSLC
                     DCAGAALVYTTARRGRVGEHMALVLAAFTAFNPLLLFDTGVWKQIDGAFALPLLLCFL
                     LLEQRRYLPAAVWFGVALAIKPQALLFGPVLAACYLAAVALEEDKHRAFGRCFGGAAL
                     ALLPPLAAGLPFFGIAQLLPKLVEKYTGTMSGYPYATINAFNWMAALGGNWKGLGETV
                     LLGITWQQLGWFNILLVTAGLAYFAVRSARGGSFSPLLLAAYYGIGVFTLAHCMHERY
                     MVPGVLLTLLAAAHWNDIRLYAAGVGLSLTGFINLATVYSLTGSDDEWLTSATSSTVA
                     ILTGLVETVCFVLLLFAMWDITRHSHTLPLPEREPEQSPPQPAAQPKWTRREVGALLA
                     LTAATAVLSFAYLGSRTAPQNPLDATDTVLTETVTPQGDAVSLWVYPGISFGGSMTVT
                     DAARNVVFEKELNYGTCFSWTANDVAAASGQPLTVTVQNAQLFELAFRDAAGQLVPAA
                     GGGALLDEQAAVPDTISQLNSMYFDEIYHGRTGYEQLHKMPVYETTHPPLGKDLIMVG
                     IAMFGMTGFGWRFSGTLFGVLLVPLAWCFVRRLTRKPWVAAVSGVLLALDFMRFSQSR
                     LATIDVYGTFFILLGAYFMLWYCQQVLTAGVDSALLPMALGGLAFGLGCAAKWTGIYA
                     GAGLAVLYLGVLYARWRQKQPGFWAEFRTAAVGGVLFYVLLPLCIYIASYLPYWWRDP
                     SFSLGDWWQCQVSMYSYHSTLQATHPFESRWYTWLLGLRPVWYYRNGYLPYGTKASIV
                     GMAGPVIWLAGLAALVGLLWRQVSGRGSRQGAGVLILYGTQLIPWMLVSRCTFLYHYF
                     PSSMFCLAALALVLARMKRRDWARRIAGGLCVAAAVLFVLYYPALSGLPIPAWWADAL
                     EVLPSFGFY"
     gene            complement(12448..13845)
                     /gene="cysS"
                     /locus_tag="ACZPAX_00450"
     CDS             complement(12448..13845)
                     /gene="cysS"
                     /locus_tag="ACZPAX_00450"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015564660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HIR1112398.1"
                     /translation="MQIFNTMTRQKEEFTPQKEGEYRIYVCGPTVYNYIHIGNARPMI
                     VFDTLRRYLEYCGNKVYYVSNITDIDDKLIKKGQEEGTSMQEVARKFEAEYIKDSDGL
                     NVKTPTVRPRATEHIGEIIHIVKDLVESGHAYVARNGDVYFRVKSDPGYGKLSHLNLD
                     DLESGNRALRSQMDDDLKEDPADFAVWKAAKPGEPYWESPWGHGRPGWHIECSAMARK
                     HLGKTIDLHAGGQDLIFPHHENEIAQSECANGCTFSRYWMHNGFLNINNEKMSKSANN
                     FFTVREIADKYGYEPIRYFMLTAGYRMPLNYTVELIESCKNSLERMYTCRDNLDFAIE
                     HAHGTDTALTEKCAEARKKFKAAMDDDLNTPDALAAIFDLVKDINTLSDVSDKATLEE
                     AAKTMDELTGVLGLLYNRKKTDSIPAEVTALVEERAAAKKAKDWGRADAIRAQLTEMG
                     WSVTDTAQGPKIAKL"
     gene            complement(13919..15856)
                     /locus_tag="ACZPAX_00455"
     CDS             complement(13919..15856)
                     /locus_tag="ACZPAX_00455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1112399.1"
                     /translation="MAKLRTKLYTLYRRMTRLERAVLVLLCVFAVKFVCSTAAHFVPG
                     LQAADMLGSGRNPVDIWLLLLSCAAVLGVFCLYRRAARAVGAKLTKADVVILGICIAA
                     SAAFYLHAMVGRQSLYLWDNATYYNLQVRLESNFADGVFTGVGSTIYKTWFNDYAPLV
                     INLLAEPFFMFTPRTANTFALLCALLIPTLVYYSAWIFLTVFRQKMQPKAPRLFTALS
                     MAFVLLLPLLHIALYRGMPDLLGVAFAFMLFALGVGYDFSDPAPARLVSLAAFTGLLM
                     LTRRSYMFTVVAFYLLYGVWVLARAARAKQGSAALRFVKFAAASLVCVGVPLLPMFWR
                     IVRADYSDRYATYQTGGFLAELNNQRVYLGWLVFAIMLVGILYGLYKSKARSLSVLSA
                     VGAVLTVLLVTRVQNMDDHQSLAVAPFYLLGCFLCALLIANLPWAWPRRAVAAVLGVL
                     CALNFGACSQLLPVILPNAVNSGLYFYVDSPRADLAQVAEVNAWLRENCTGENSAYMI
                     CHGVVYSPDVFRVSALPDETIREILPYGVCNPGNDAFPKELFTAQVVLTCTPFDPNNH
                     TEKMDAAFLENQEKYAPFEIAAEFDMGNGYTITAYRRIKAPTVAELDTYRSYLAEEDA
                     RFPYNFSAVWDELAAQFANNG"
     gene            complement(15856..16431)
                     /gene="pgsA"
                     /locus_tag="ACZPAX_00460"
     CDS             complement(15856..16431)
                     /gene="pgsA"
                     /locus_tag="ACZPAX_00460"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015537256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HIR1112400.1"
                     /translation="MNLPNKLTMMRVVLVPVFMIFAALSHYGTADFNAAFMLTAGIIF
                     AVASFTDFLDGYLARKNNLVTDFGKFMDPLADKCLTTAAFIYMVTDGVCSPIVLAVIL
                     FREFAVAGVRMLAAETGTVIAANMWGKVKTVLQMLTIIFYYFGGALAGPTDVGVVSFV
                     TVVLCWLCGAATVLSGGIYLWQNRKLFMQAK"
     gene            complement(16435..17775)
                     /gene="rimO"
                     /locus_tag="ACZPAX_00465"
     CDS             complement(16435..17775)
                     /gene="rimO"
                     /locus_tag="ACZPAX_00465"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HIR1112401.1"
                     /translation="MKIAIISLGCPKNQVDADVFCHALIKDGHTTVGDPAEADIIIIN
                     TCGFIESAKTEAIENILMACQYKQQNPALKVIVTGCLAERYKQQIIQEIPEVDAVVGI
                     GSNAALPAIVRRLCADGAGQTESYGPKSDMPLGGARVISTPRHYAYLKIAEGCNNGCY
                     YCAIPLIRGALRSREINDCVAEARWLAGEGVRELILVAQDPTAYGEDWGKPGAICELL
                     DRLQEIDGIRWIRVLYAYPERISDEFIAALVRNTKVVPYLDLPIQHCDDAVLKAMNRR
                     GGRKEIEDAIARLRAAIPGITLRTTLIAGFPGETEEQYAELCDFVKTVKFDRLGCFAY
                     SAEENTVAAKMPNQLDEETKQRRADHIMELQAEVSAEREGEKVGSTYECICDGIDEET
                     GMYLLRTKADCPEIDGNVLTPADTPLETGAFYNVTITDSDTYDLYGYVEEKLED"
     gene            complement(17874..18491)
                     /locus_tag="ACZPAX_00470"
     CDS             complement(17874..18491)
                     /locus_tag="ACZPAX_00470"
                     /inference="COORDINATES: protein motif:HMM:NF014668.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HIR1112402.1"
                     /translation="MLRLTQIKETKKGRLALFFDGEFAFSLDENTFADAGLHQDDDLE
                     EWQIEELRKKSETRRALDKAMDYLALRDHAAGELYQKLCRKFDAHSAAYAVARAGELG
                     LLNDAGFARRRAAELLRKRKSRREILNDLSAKGIDRDTAAEVVKELFAEGEDGENPEL
                     ATARALVERQYAAKLAAGKRDQVAAALVRRGFSHAVIREVLSDDE"
     gene            complement(18495..19667)
                     /gene="recA"
                     /locus_tag="ACZPAX_00475"
     CDS             complement(18495..19667)
                     /gene="recA"
                     /locus_tag="ACZPAX_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HIR1112403.1"
                     /translation="MAAKKDTTPKTAGPAADKKAALETALAQIEKQFGKGAVMKLGAN
                     VTMQVDAIPTGSLGLDLALGIGGVPRGRIVEVYGPESSGKTTLALQILAEAQKMGGEV
                     AFIDVEHALDPTYAAALGVDIDSLLVSQPDTGEQAMEICEALVRSGAIDAVVVDSVAA
                     MVPRAEIEGEMGDSHVGLQARLMSQALRKLTGVIGKTNTVCIFINQLREKVGIVYGNP
                     EVTTGGRALKYYSSVRIDVRRIEGLKDSTGAFIGNRTRAKIVKNKVAPPFREAEFDIM
                     FGEGISKIGEILDLGVKLGVVQKSGAWFNYGEMRLGQGRDNAKQFLKDHPEVSDEIEK
                     IVRANADRLLAAGKKGTVKPLDPSEIVKPVAAGEAPAAPAAKTTGSEVDLDIMVDE"
     gene            complement(19801..20619)
                     /gene="prmC"
                     /locus_tag="ACZPAX_00480"
     CDS             complement(19801..20619)
                     /gene="prmC"
                     /locus_tag="ACZPAX_00480"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HIR1112404.1"
                     /translation="MNAAFQALCARLAAAGVPDARFDAAELYRFVTRRDPRLGDGPTE
                     AEAARLAALADRRAAREPLQYILGEWDFMDFSLKVGPGVLCPRADSEVVCETAIELLQ
                     GVPSPRVYDLCAGTGCLGLGIARHVPGADVTCVEKSPDAWPYLTANTADSGVRTMQAD
                     VFTYYKLLSEECADLIISNPPYLTGPEMGALMPETAHEPAMALDGGADGLDFYRLLTT
                     RYKAALRPGGWLVMEIGYAQAADVLALGEIHGWANGSCRKDYGGNDRVVLLQKN"
     gene            complement(20616..>21021)
                     /locus_tag="ACZPAX_00485"
     CDS             complement(20616..>21021)
                     /locus_tag="ACZPAX_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005934887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HIR1112405.1"
                     /translation="QYHGAEHKTIFCYEAGLPLTVENVRRQPRHHPRCGTSFMILVII
                     ISIFLYAVLPWTSTALRVVYKLCMFPLLVGISYEILKWAGRSDSLLSKIVSQPGLWMQ
                     RLTTFEPDDSMIEVAIAAVTPVLPKNQEEARW"
BASE COUNT         4227 a         6427 c         6268 g         4099 t
ORIGIN      
        1 ggccaacggt tcggggcatc gtggaggccg acccctacat cttcgtaggg gcggattcta
       61 tatccgcccg cggtacctgc cgtaagctgc acgggcgcat atggaatgcg cccctacggc
      121 ccgatttgca acgacagata taaccaccaa aagggaaggc tgcgtttcta ccgcagtctt
      181 cccttgatga ctcaatcaga atcaggcgac agggtcagca tcctcaatga tgccgtcgat
      241 gcgcagggag aaggtcgggg ctgcgcgcag ctcgccctcg tggaagtaac cgtcggagat
      301 agcctccttg gtctcaccct ggtacacggc aaccgggctg ccggtgctga aatcgtagta
      361 ggtcaggtcg atgggggcgg tggtgacctc cgcgccgtcg acggtgaact tgctggtgtc
      421 gaagacatgc aggctgccgt ccttcagcgc agcctcaacc tcggcgacct tctcggcggt
      481 gccgtcggca acttcggggc caaggtcggt gatggcgaca gcgccatcct catagccctt
      541 gcaccagtcc tgcgggatgc tgccggcctg cgcaccctcg aacagctcct tgtagtagac
      601 cttccagacg ttcgtcgcgg aggtcagggc agcggtgggg gcggtggcaa gcatatcgat
      661 gttgtagccg atggagtagc agatcttgcc ggagtctttc agcttctggg tggcagcggg
      721 ggcaccggtg gagtcagcgt gctggccgat gatgaccgca ccgttggcga tcagggcctc
      781 ggcggcagcg ccctccttgt cgatgtcgaa ccaggagttg gtgtacatga cttccatctg
      841 gacatcgggg taaacgctct ggacgcccag gaagaaggcg gtgtagccgg agacgacctc
      901 ggcgtagttg taagcgccca cgtagccgat cttgaccttg ccgtcagcgg tgaagctgtc
      961 gggctgggtc tcgggggtca gcgtgccgga ctcgaccagg tctttgacct tcatgccggc
     1021 gacgacaccg gagacgtagc ggctctggta aacggcggtg aaggcgttgt agaagttgtc
     1081 aaggccggag atggcggcga agtcgccggt catagccacg aagttgatat cagggtactt
     1141 ctcggcttcc tcctccatgt aggtctgatg gccgtaggag ttggagatga tcaggttgca
     1201 gccctggccg acaaggtcct cggctgcgtc ggcggtgacg ccgccctcag cgatcttgta
     1261 cttccagacg atctggtcat cggacatgcc cagctcggcg gcagcttcct tgatgccgtt
     1321 gatgtgcgca gcgctgtaac cttcggtctc atcgcccagg atgatggcac cgaccttgac
     1381 gtcgggggcc tggttgtcgc tgctgccctc agcagtagcg gcagaggcag tggcagtgct
     1441 ggcagcggtg tctgcagtgg aaccacaggc agccaggctg aacaccatag aggtggccag
     1501 cagaataccc atcaatttct tcatgtgtgt tgttcctccc tgttgtgtgt gcataaatag
     1561 caaaaatgcg tttcattttg tcggaacgtg ccggaaatca acgcattttg taaccttgca
     1621 tgacccattg tagacgcttt acaaaccgtt tgcaataatt tttaaatttt tgtgaaaagt
     1681 tcagcgaaaa gggtaaatgt tttgcccgcg tgcacacaac aacgggcaga acgcacaaca
     1741 aaaaaaagta aaagtcatac tttgttgtgc aaaagtatga ctttttgagg actttgtata
     1801 agtaccggaa ctattacgat actagggata ttttggcgtt tttgcttttt acgccaagct
     1861 caccatccac tttctcttac ggaacaccac cagcgacacc acaaggcgga acagttcctc
     1921 cgccgtcagc agcgtgtaca cgcccacaat gccccagtgc agtacatagg ccgcaaaaaa
     1981 gcacagcgga atgccgatgc cccaggtgcc cgctatgtcg atgatcatga ccatccgggt
     2041 gttgccgccg cttttcagga tgccgccgcc caaaatcatg ttttccactt taatgggcgc
     2101 atacagcgca aacaccacca gcagcatatg gcccagggtg cggacttcgc cttccacgcg
     2161 gtacagcccc acgtactgcc cggccagccc gaccagcagt gatgaaacca ccgctgcgcc
     2221 caccagcccg gtgaccatga tcttcttggc gtcgccgtag gctgcgtcat actccttgcg
     2281 gcccagccgc ttgccgatca gcacgcccgc cgccgcggaa agtccgctga gcgccccgac
     2341 gatgaggctt tgtatcgggc aggtcagcgt gtaggccgcc agattctgcg tgcccaggtg
     2401 tccatacacg gcggattcca cgttctggcc caggctccac aaaaactggc tgaccagaat
     2461 cggcgtgatc atgcccagat agtcccgccg ggtgattttg gtaaaatgca gcgagggctg
     2521 cacccgctcg ccgtcctgcc gcagacagtg taggaatccc gtcgcgatga gcgccagatt
     2581 taccagctgc gacaccagcg tggcaatcgc cgccccgcgc accccgcagg cgggcaggcc
     2641 cagcttgccg aaaatcagca catagttcag caccacgtta gaaagcaccg cgccgatgct
     2701 ggccgcgaag ggaatgttcg cgtgccccct gcagcgcagc caggcagaca agaccgtgga
     2761 aaggcccatg ggcgcgaagg agaacgcgat aattttaaag tagacgcatc cctcggcgat
     2821 gatggcagca tcctttgtgt acagccccag aatctgcgcc gtcgccccca gtgagacgcc
     2881 caggaacacc cccgccaggc acaggcttat cacggtgctc agcgtcaggc tgctccacgc
     2941 ctctttttta tcccctgcgc cgagaaattg ggcaatgaga atgcccgcca ccgtgcccac
     3001 cgagccgctg actacattga aaatcatcga atagttgccg cccagcccca cagccgcgat
     3061 gctgactgcc cccagctggc caatcataat ttggtccacg atagaaaacg acgcctgcag
     3121 catactttgc agtgtgaccg gtacagcaat tttgcccagt tcccttataa attttgacat
     3181 gatacgcctc ctgtgctctc cctcggcagc aatgtgaccg gcagtacgat cttttccgcg
     3241 cagctctggc cgcggcccat ggcctcgata ctggcgacgg caatgcccgc ccgcagcgcg
     3301 gcgtcctgcc gtaccgtcgt cagcgacggg tatccggtgc ggctggcctc gctgtcgtca
     3361 aagccaacaa cggccatatc ccccggcacc tgcagcccct tggcctgcaa aaagcgcata
     3421 cattccagcg cgtaaaaatc cgacactgcg aagatggccg tgatgccgga tgtgcgcaga
     3481 gctgcgaaat tttcttcata aaactgctgc cgtggccggg cctgcaccgg aacctgccag
     3541 cgggtcacac ggtcggggcc aaaggccgcc gcaaagccgt ccattcgttc cttgtccatg
     3601 cagatattgt tatctgccag gcagagtgcc cgggtgtggc ctgcggcttt caggtattgc
     3661 ccggcctgcc gcccgccgtc ggcgtggtcg atcacgagat tgaccacacc cggacagccc
     3721 tgcatatagc cgtcgtacac gacgatgggc gtgcggatgg cctggcgcag tgcggcatag
     3781 tcagcctcac agaagcccat cagaatcagc ccttccatcc gccacatcga cgcaaacgcc
     3841 gggacctcat ccagtgtccg ggtggtttta accatcaaga agaagccctt ttcgtttgcc
     3901 tcgcgggaga gtgcgttcag cgacgccgtg acgaagccat cctccaggac tttgccctcg
     3961 tacttggggc tgtcatcgac gaccacgccg atgatgcggg agcttttctg cgccagccag
     4021 acgcccgcca tatcggggat atagcccaca gcttccagct cctgccagac gcgctgggcc
     4081 gtgcgcggtg acattttgga cgtcttgccg tggatgacgt tggacactgt ggccgtgctc
     4141 agccccagcc gctctgcgat atccacaatg cggatacgat tttcctgcat ggtgcgcccc
     4201 ctctctgtac ttccattatg caacgcacta ccattatacg gcagaggccg ccccagggca
     4261 aaggttaaac gcgtaaccac gtacagaaaa tttccgttaa ttttggtgaa aacgccagca
     4321 ccgggcatac tttaccgctt atgaataaac ttcctgtcca atccctgctt ggcggcggcg
     4381 ttcaggacga cccaggtcag gaatacctcg atgggcagca tgatcgtgtt tttcaccgca
     4441 ccggaggtca ggtagtacag gtaccccttg ccgtagagca tggctttcca cacgctgccc
     4501 aacaagacat tgctgccgta gttgatgatg agccgcgtca caataatgcg cggcagcgtc
     4561 ggtttgcggc ggtagagcat aacgccgtag atcagccccg acagcaccgc cgtgacaagg
     4621 tagccgggaa aatacggctc gccgtagccg gagagcagaa agccgacgct gtcaatgatg
     4681 gcaccggcca tcatgcccac gaccgggccg tagcagctgc atcccagcga gacggccaaa
     4741 aaactaaagt agattttcag gctcggcccc atcaggtaga tgggcaggct ttccagcacg
     4801 atggccatag cgcagaccag gcccgagaac gccagcacgc gggggttgtt cagttcccgc
     4861 cgggcggtgc gccagtagtc gggggtgagg acggacaggg tttgttcgga tacagtgcac
     4921 ataaaaaatc ctcctttgtg cgtttgccgc acaagggagg aggcaatttt ggttgggacg
     4981 ggcacaacgc cgcatccgcc gttgcacacc atcatgcagc agtgggatgc ggaacccgca
     5041 ccgcgaacct tgcggtcact tcgtcctgcc acaactcccc gttggtcagg cacttcgggg
     5101 cagcgcccca cgcgcggatt cctactctgc gatatcgttt tttattatac acgggttttg
     5161 gggaaatgca aggggaagtt tttcttccgc tgagccttgg gaattttact gcccgctcgc
     5221 cccatagttt gccagcactc tcgcgctggg gtcgttgacg tcgcagtgag gccatgcctt
     5281 gttgcgcatc cagtaatcga caatcatctc cgcctggccg gggtagtact tctcaaaaat
     5341 ttcgcggtac agcaggcttt ccttggtaaa ggggcggcca taggtgtact tgccgcactt
     5401 ctcggcaaac tcgcggtcag tgtagatcgt ctcggcatac tccttcagat cgtccaccat
     5461 cgagtggccc accgcgtcgc tgaaggcggc cttctcgcgc cagaggatgc tctcgggaag
     5521 ccagtcgccc tcgaaagcct tgcgcagcag gtacttaccc ttgccgtagg tgttcagctt
     5581 cttctcgggg gcgatggcca tgacgtactt gacgaaatcc aagtcgccga aaggcacgcg
     5641 ggcttccagg ctgttgacgc tgatggaacg gtcggcgcgc agcacatcgt acatatacag
     5701 ctcgcggatg cgcttctggc tctcggcctg gaacgcggcg gcgctgggtg cgtagtcggt
     5761 gtacttgtag ccgaacagct catcggaaat ctcaccggtc agcagcacgc ggatgtcggt
     5821 gtgctcgtgg atgtacttgc agacgagata catacccacc gacgcgcgga tggtcgtgat
     5881 atcccaggtg cccagcatgg caatgacgct ttccagcgca tccaagacga tgtccttgtt
     5941 gatgatgacc tcggtgtgat cggcgtgcag gtagtcagcc acctgcttgg cgtatttcag
     6001 gtcgatggcg tcctcgctca tgccgatggc aaaggtgcgg atgggcttgc ccagcttttt
     6061 ggccgcaatg gcgcagacca ggctggaatc cagaccgccg gagagcagga acccgaccgg
     6121 cgtgtcggca tccagccgct tgtccacacc cgcgacgagc ttttcccgga tgttcgtcaa
     6181 tacatcggga agttcatcct ggctgtacgc gggggtgtcg gcgatatcgc agtaccgcac
     6241 gaactgacca ttgcagtaat agctgccgat ggggaacgga tagatttttt tgcatagccc
     6301 cacaaggttt tttgcctcgg acgcaaaggc aatgcggccg ctctcgctgt acccgtagaa
     6361 cagcgggcgg atgccgatgg ggtcgcgggc agcgatgagc agctttttcc ggctgtcgta
     6421 cagaatcatc gcaaactcag cgtccagatg cttgaacata tccaggccgt atttgtagta
     6481 gagcggcagg atgatctcgc agtcggagtc ggactcaaag gtgtagccct cggcttccag
     6541 ttcggctttg acggggcgga agccgtacag ctcgccgttg caggcaacgc aatccgcacc
     6601 gcggaagaag ggctgcatgc cctcgggcgt caagcccata atggcgagac ggccaaagcc
     6661 cagcagaccg aactcagtct cggccacacg ctggtcgtcc ggcccgcggc tggtggtacg
     6721 cagcagatac tccgtaaacg tttccttggg cagatcatgc ccggcataac ccataatgca
     6781 gcacattgtt tgtttctcct tttcggtctt acactttccg aatggtaaaa caaaaagaca
     6841 gcccattcgc cctgtcatta ggacgaatcg ctgtcttgat ccgcggtgcc acctaaatta
     6901 ccgcagccga aataagggca tacggtcact ttttccgtac acgttcacct ttgaaacgct
     6961 gccgctgtaa cgcacagcct gcggcgcacc ctactttttg catttcaggc tgcagctccg
     7021 gggtgttctt tcgcgccctg tcaccgtccc ggcttacacc aaatgccagg ctctctgtgc
     7081 gggtcttggg cggtactttt ccccatcaac gcttttgttg agtaaagaat aacacggtca
     7141 tgcagtcctg tcaagaataa atttcagatt tttgggcgtc cgccctgttt ttttcagcag
     7201 gtcggcggca cggcgggtgc gccgtgcttc gtcatcttgc acaactttgc cccggtttcg
     7261 caatttttgc aaggttgtcg aataattctt gcaaatattc gtgatgcaaa attccatact
     7321 atggaacgct attccatctg ccgaattttg ccgcgcaccg cagttccacc ctgcggaatc
     7381 gcatcgttct gccgcccgca ccttcatcta agcaaccgcc gcaatggcca aaaccgccgc
     7441 cgcatggtac actgaggatg acaatattcg tgtgctctgc gccgcgcctc taccctgcgc
     7501 gggcacgcca cccacaagct gcaggccctg cctgcaagga ggaaaactat ggcaaaaaat
     7561 gcgatcgtcg gtcagtccgg cggcccgact tccgtcatca acgccagtct cgcgggcgtg
     7621 tatgaaagct gcaagcgccg cggcgcgggc aaggtctacg gtatgctgca cggcgtggcc
     7681 ggcttgttag agcgccgcac ctgcgtactg gacgacaagc tgaaaaccgc actggacatt
     7741 gagcttttga agcgcacgcc gtccagctat ctgggttcct gccgctacaa gctgcccgac
     7801 tggcacaccg ccgagggcga ggccgtctac atccagctgt ttgagatcct gaaggagctg
     7861 gacatcggct acttcttcta catcggcggt aacgactcga tggacaccat cggcaagctg
     7921 gccgactacg gcgcacacat cggcagcggc atccgcttta tgggcgtgcc taagaccatc
     7981 gacaatgatc tgatggtcac cgaccacacc ccgggctacg gcagcgccgc caagtacatc
     8041 ggtgtcgtga tgaaggagat catccgcgat gcgacagtct acggcaccaa gtacgtgacc
     8101 attgttgaaa tcatgggccg caacgcgggc tggctgaccg ccgccgctgc attggccaag
     8161 gccgaggact gcgagggcgt ggacatgatc tgcctgcccg aagtgccctt caacgtggat
     8221 catttcgtgg agaaggtcga gcggatgcaa aaggagaagc cgtccatcgt catcgccgtg
     8281 tccgagggcg tcaagctcga ggatggccgc tacgtctgcg agctggccga cgatgtccac
     8341 gcggtggatg ctttcggcca caaggccctg accggcacgg cgcgtttcct ggcgaacacc
     8401 gttgcccgcc gtctggacac gaaaacacgc tgcatcgagc tttctaccct gcagcgctgc
     8461 gcgggtcatc tgacgagccg caccgatatc accgaggctt accaggtcgg tggtgccgcc
     8521 gccaaggccg cctttgaggg ccacaccggc gagatggtcg cgctggaccg catttccaac
     8581 gcgccgtacc agtgcggtac gagcctgcac cccatcagcg aggttgccaa cctggaaaag
     8641 aaggtgccgc tggagtgggt caacgccgac cacaccgcca tgacggatga gttcctggcc
     8701 tacgccctgc ccctggtcca ggccgagctg accccgatct acgtcgaggg cctgccgcat
     8761 catatctacc tgccggaggc gtaagtcact ttctttgaga agaaagtaac taaagaagct
     8821 taatcttaat acaataacgc cgcagcaacg ggaaatcact tccgttactg cggcgttttt
     8881 ctaaagcctc ccttgtcaaa gggaggtggc cgagcttcac gaggccggag ggattcctgc
     8941 tgccttacgg aagcagctcc atcctctcct gcaccgcttt gtcaatcgca gtgcatacgc
     9001 cttcaaaatt tttcatcaca tcattgtttg caaaccgtag cacccgcaga ccgtactttt
     9061 gcaaaaactt cgtgcgggcc gcatcgcata cacgtccggc atcctcaaaa tgctgcgaac
     9121 cgtccagttc caccaccaga tttgccgccg cacagtagaa atcaacaaca tacttgccga
     9181 caactttctg ccgcagaaag cggatgggat acccccggag aaagtcgtac caaaggtgac
     9241 gttcttcctt cgtcatatct ttgcgaagcc ttctggcaat gggagtcagc tctttgttgt
     9301 gcttgccggt cataagtaat gattcctttc ggtttctcgc aaggctgatg gactggaatc
     9361 cctcagtcgc cttatcggcg acagctccct ttgacaaggg agccttttta atagaaccca
     9421 aagctcggca gcacttccag ggcatccgcc caccaggcgg ggatgggcag gccggatagg
     9481 gccgggtagt agagcacaaa cagaacggcc gccgccacgc acaggccgcc cgcaatgcgc
     9541 ctggcccagt cgcggcgctt catgcgggcc agcaccaggg ccagcgccgc caggcagaac
     9601 atggaactgg ggaaataatg gtacaagaac gtacaccgcg acaccagcat ccaggggatc
     9661 agctgcgtgc cgtacaaaat cagcacgccc gcgccctggc ggctgccgcg cccgctgacc
     9721 tgccgccaca gcaggcccac cagcgccgcc agcccggcca gccagatgac cggccctgcc
     9781 atgcccacaa tgctggcctt ggtgccgtag ggcagatagc cgttgcggta gtaccacacc
     9841 gggcgcaggc ccagcagcca ggtgtaccag cggctctcaa acgggtgggt cgcctgcaaa
     9901 gtggaatggt aactatacat cgagacctgg cactgccacc aatcgcccag actgaacgaa
     9961 gggtcacgcc accagtacgg caggtaggac gcaatgtaga tgcagagcgg cagcagcaca
    10021 tagaacagta cgccgcccac cgccgccgtg cggaactctg cccagaagcc gggctgcttc
    10081 tgccgccagc gggcgtacag aacgcccagg tagagcaccg ccagcccggc cccggcgtag
    10141 atgccggtcc acttggccgc gcagcccagg ccgaaggcca gcccgcccag cgccatcggc
    10201 agaagggcgc tgtccacgcc tgccgtcagc acctgctggc agtaccacag catgaagtac
    10261 gcccccagca ggatgaaaaa cgtgccgtag acgtcaatcg ttgccaggcg gctttgggaa
    10321 aaacgcatga aatccagcgc cagcagcacg ccggagaccg ccgccaccca tggcttgcgg
    10381 gtcaggcggc gcacaaagca ccaggccagc ggcaccagca gcacgccgaa cagcgtaccg
    10441 gaaaaccgcc atccaaagcc ggtcatgccg aacatcgcga tgcccaccat gatcagatcc
    10501 ttgcccagcg gtgggtgggt cgtctcgtag acaggcatct tgtgcagctg ctcataaccg
    10561 gtacggccat ggtaaatctc gtcaaaatac atactgttca gctggctgat agtgtccggc
    10621 acagcggcct gctcatcaag cagcgcaccg ccgcccgctg ccggcaccag ctgccccgcc
    10681 gcgtcgcgga aggccagctc aaacagctgg gcgttctgca cggtcacggt caggggctgc
    10741 cctgacgcgg cggccacatc gttggccgtc cagctgaagc aggtgccgta gttcagttct
    10801 ttttcaaaga ccacattgcg cgcagcgtcg gtcacggtca tactgccgcc gaaggaaatg
    10861 cccgggtaca cccacagcga gaccgcgtcg ccctgcggcg tcaccgtctc ggtcagtacg
    10921 gtatcggtgg cgtccagcgg gttctgcggc gcggtgcggc tgcccagata ggcaaagctc
    10981 agcaccgccg tggccgccgt caatgccagc agtgcgccca cctcgcggcg ggtccatttc
    11041 ggctgtgcgg caggctgcgg cggggattgc tcgggttcac gctccggcag gggcagcgtg
    11101 tggctgtggc gggtgatatc ccacatggca aacagcagca gcacaaagca gaccgtctcg
    11161 accaggccgg tcaagatcgc caccgtggaa ctggtggcgc tggtcagcca ttcgtcgtcg
    11221 gagccggtca gcgagtagac cgtggccagg ttgatgaacc cggtcagcga caggcccacc
    11281 ccggccgcgt aaaggcggat atcgttccaa tgcgcagcgg ccagcagggt cagcagcacg
    11341 ccgggcacca tgtagcgctc atgcatacag tgggccagcg tgaacacacc gatgccatag
    11401 tacgccgcca gcagcagcgg cgagaagctg ccaccccggg cgctgcgcac cgcaaagtag
    11461 gccaggcccg ccgtgaccag caggatattg aaccacccca gctgctgcca ggtgatgccc
    11521 agcagtaccg tctcgcccag gcccttccag ttgccgccca gcgccgccat ccagttgaag
    11581 gcgttgatgg tggcgtaagg gtagccggac atcgtacctg tgtacttttc taccagcttg
    11641 gggagcaact gtgcgatgcc gaaaaacggc agccccgccg ccagcggggg cagcaatgcc
    11701 agcgccgcac cgccgaagca gcggccaaag gcgcggtgct tatcttcttc cagcgcgaca
    11761 gccgccaggt agcaggccgc cagcaccggc ccgaacagca gcgcctgcgg cttgatagcc
    11821 agcgccacgc cgaaccagac ggcggcgggc aggtagcggc gctgttccag cagcaaaaag
    11881 cagagcagca gcggcagtgc gaatgcgccg tcgatctgct tccagacgcc ggtgtcaaac
    11941 aatagcagcg ggttaaacgc tgtgaacgcc gccagcacca gcgccatgtg ctcccctacc
    12001 ctgccgcgcc gggctgtggt ataaaccagc gccgcccccg cgcagtcgca gagcgacggc
    12061 acaagcgtca gcagaaagta ggtccatttg gactcgtagg caatgtgcag cgcggcgcgg
    12121 atggccccca ccagccccag cacccacagg tagccgggcg ggtagtcggc aaagtagccc
    12181 tcgctgtaaa aggcggcggg gccctcggcg gccagcttgt caccccaggc cacaaagcag
    12241 ctcatgtcgt aggggtagcc ctcggtcaca agggccagca ccagccgcag tgccagcccg
    12301 cagcccagca acagccaaag ggttatgttt tgtttttcgc gttgtttcgt catatcatca
    12361 caaattcctg gtacaaaagc aggggccagc ccgcgtaccc gcgccctgcg gcgcagatgc
    12421 gcaggccgcc cctgcatctg tttttgctta cagttttgcg attttcgggc cctgggcagt
    12481 gtcggtgaca ctccagccca tctcggtcag ctgggcacgg atggcgtccg cacggcccca
    12541 gtccttggcc ttcttggcgg cggcgcgctc ctcgaccagc gccgtgacct cggcggggat
    12601 gctgtcggtc tttttgcggt tgtatagcag gcccagcacg ccggtcagct cgtccatcgt
    12661 cttggcggct tcttccagcg ttgccttgtc ggagacatcg gacagcgtgt tgatgtcctt
    12721 gaccaggtcg aaaatagcgg ccaaagcatc gggggtgttc aggtcgtcgt ccatggcagc
    12781 cttgaacttt ttacgcgctt ctgcgcactt ttccgtcagc gcagtgtcgg tgccgtgggc
    12841 gtgctcaatg gcgaaatcga ggttatcacg gcaggtgtac atacgctcaa ggctgttctt
    12901 gcagctctcg atcagctcga ccgtgtagtt cagcggcatg cgatagcccg ccgtcagcat
    12961 aaagtagcgg atcggctcat agccgtactt gtcggcgatc tcgcgcaccg tgaagaagtt
    13021 gttcgcgctc ttggacatct tctcgttgtt gatgttcagg aagccgttgt gcatccagta
    13081 gcggctgaag gtgcagccgt tggcgcactc gctctgggcg atctcgttct catgatgggg
    13141 gaagatcaga tcctggccgc cggcgtggag gtcgatggtc ttgcccaagt gcttgcgggc
    13201 catggcgctg cactcgatgt gccagccggg acgaccgtgc ccccaggggc tttcccagta
    13261 gggctcgccg ggcttggcag ccttccagac cgcgaaatcg gcgggatcct ccttcagatc
    13321 atcgtccatc tggctgcgca gggcacggtt gccgctttcg aggtcgtcga ggttcagatg
    13381 gctcagcttg ccgtagccgg ggtcagactt gacgcggaaa tagacgtcgc cgttgcgggc
    13441 cacataggcg tggccgctct cgaccagatc cttgacgatg tggatgatct cgccgatatg
    13501 ctcggtggcg cgggggcgca cggtgggggt cttgacgttc aaaccgtcag agtccttgat
    13561 gtactcggcc tcaaacttgc gggcaacctc ctgcatcgag gtcccctcct cctggccttt
    13621 cttgatgagt ttgtcatcaa tgtcggtgat gttggagacg tagtagacct tgttgccgca
    13681 gtattccagg taacggcgca gggtgtcgaa cacgatcatc gggcgcgcgt tgccgatgtg
    13741 gatataatta taaaccgtcg ggccgcagac atagatgcgg tattcgccct ccttctgggg
    13801 ggtgaactcc tctttctggc gggtcatggt gttgaagatc tgcatagctt gcacgttcct
    13861 tcctctatat agggatacct ttattatagc caacacacgg cgcggttgca agggggattc
    13921 agccgttatt tgcgaattgg gcggccagct cgtcccacac ggcgctgaaa ttgtaaggga
    13981 accgcgcgtc ctcctcggcc agatagctgc ggtaggtgtc cagctcggcc acagtcgggg
    14041 ccttgatgcg gcggtaggcc gtgatggtgt agccgttgcc catgtcaaac tccgctgcaa
    14101 tctcaaacgg cgcgtacttt tcctggtttt ccaggaaagc ggcgtccatc ttctcggtgt
    14161 ggttgttggg gtcaaacggc gtgcaggtca gcacgacctg cgccgtgaac agttccttcg
    14221 gaaaggcgtc gttgccgggg ttacagacac cgtagggcaa gatttcgcgg atcgtctcat
    14281 ccggcagcgc cgacacgcgg aatacatcgg ggctgtacac aacgccgtga caaatcatgt
    14341 aggcggagtt ctcgccggtg cagttttccc gcagccaggc gttgacctca gccacctggg
    14401 ccaggtcggc gcgagggctg tccacataga aatacagtcc gctgttgaca gcgtttggca
    14461 gaatgaccgg caaaagctgg ctgcacgcgc cgaagttgag ggcacacagc acgcccagca
    14521 ccgcagctac ggcgcggcgg ggccacgccc agggcaggtt ggcgatgagc agcgcacaga
    14581 ggaagcagcc cagcaggtag aacggtgcga cggccaggga ttgatggtcg tccatattct
    14641 gcacccgggt cacaagcagc acggtcagca ccgcgccgac agcggagagc accgacaggc
    14701 tgcgggcctt ggatttatac aatccgtata gtatgccaac aagcatgatt gcgaacacga
    14761 gccagcccag atagacgcgc tggttgttca gctcggccaa aaagccaccg gtctggtagg
    14821 tggcgtaacg gtcgctgtaa tctgcgcgga cgatacgcca gaacatgggc agcagcggca
    14881 cgccgacgca gaccagcgat gccgcagcaa atttcacaaa acgcagggcc gcactgccct
    14941 gtttggcgcg ggcggcccgg gccagcaccc agacaccgta gagcaaatag aacgccacaa
    15001 cggtaaacat atagctgcgg cgggtcagca tcagcagccc ggtgaaggcc gccaggctga
    15061 cgagccgtgc gggggccggg tcgctgaaat catacccgac gcccagcgca aacagcatga
    15121 aggcgaacgc cacacccagc aggtccggca taccacggta gagcgcaatg tgcaaaagcg
    15181 gcagcagcag cacaaacgcc atggacagcg ctgtgaagag ccgcggcgct ttcggctgca
    15241 ttttttgacg aaacaccgtc aagaaaatcc acgcactgta atagaccagc gtcgggatca
    15301 gcagcgcaca gagcagcgcg aaggtgttgg ccgtgcgggg cgtgaacatg aaaaacggct
    15361 cggccagcag gttgatgacc agcggcgcgt agtcgttgaa ccaggtttta taaatggtgg
    15421 aacctacgcc ggtgaacacg ccgtcggcga agttggattc cagccgcacc tgcaaattgt
    15481 agtaggtggc gttgtcccac aggtagaggc tttgacggcc caccatggcg tgcaggtaga
    15541 acgccgcgct ggccgcaatg cagataccga gaatgacgac atctgccttt gtcagctttg
    15601 cgccgacggc ccgcgccgcg cggcggtaga ggcagaacac gcccagtacc gccgcgcagg
    15661 agagcagcag cagccagata tccaccggat tacggccgct gcccagcata tccgcggctt
    15721 gcagccccgg gacaaaatgc gccgcggtgc tgcacacaaa cttgactgcg aacacacaca
    15781 acaaaaccag gacggcccgc tccaggcggg tcatcctgcg atacaacgta taaagtttcg
    15841 ttcggagttt tgccattatt ttgcctgcat gaacagcttg cggttctgcc acaggtaaat
    15901 gccgcccgaa agcacggtgg ccgcgccgca cagccagcag agcacgacgg tcacaaagct
    15961 gacaacaccc acatccgtcg gcccggccag cgcaccaccg aagtagtaga agatgatggt
    16021 cagcatctgc agaacggtct tgaccttgcc ccacatattg gcggcaatga ccgtgccggt
    16081 ctcggcggcc agcatacgca cgccggcaac cgcaaactca cggaacagga tgaccgccag
    16141 cacgatgggc gaacagacgc catccgtaac catataaatg aaagcggcag tagtcaggca
    16201 cttgtcggcc agggggtcca taaacttgcc gaaatcggtg acaaggttat tcttgcgggc
    16261 caggtagccg tccagaaaat ccgtgaagct ggccacggca aagataatgc ccgctgtcag
    16321 catgaatgcg gcgttgaaat ccgccgtgcc atagtgggac agtgccgcaa atatcatgaa
    16381 cacaggcacc agaaccacac gcatcatcgt caatttatta ggtagattca tacatcaatc
    16441 ctccagcttt tcttccacat agccgtacag gtcgtaggtg tcggaatcgg tgatggtcac
    16501 attgtagaac gcgccggtct ccagcggggt gtcggcagga gtcagcacat tgccgtcaat
    16561 ttcagggcag tcagccttgg tgcgcagcag gtacatcccg gtctcctcgt cgatgccgtc
    16621 gcagatgcac tcgtaggtgc tgccgacctt ctcgccctcg cgctcggcgc tgacttcggc
    16681 ctgcaattcc atgatgtggt cggcgcggcg ctgcttggtt tcctcgtcga gctggttggg
    16741 catcttcgcg gccacggtgt tttcctcggc cgagtaggca aagcagccca ggcggtcaaa
    16801 tttgacagtc ttgacaaagt cgcacaactc ggcgtactgc tcctcggtct cgccggggaa
    16861 gcccgcgatc agcgtggtgc gcagcgtgat accggggatg gcagcccgca gccgggcgat
    16921 ggcatcctcg atctccttgc ggccgccacg gcggttcatc gctttcagca cggcgtcgtc
    16981 gcagtgctgg atgggcaggt caagataggg cacaaccttg gtgttgcgga cgagggccgc
    17041 aataaattca tcgctgatac gttcaggata ggcgtacagc acgcgaatcc agcggatacc
    17101 gtcgatttcc tgcaagcggt ccagcagctc acagatggca ccgggtttgc cccagtcctc
    17161 accataagca gtggggtcct gggcaaccag aatcagctcc cgcacgccct cgccagccag
    17221 ccagcgggcc tcggccacac agtcgttgat ctcgcggctg cgcagcgcac cgcggatcag
    17281 cgggatggcg cagtagtagc agccgttgtt gcagccctcg gcaattttta agtacgcata
    17341 gtggcgcggg gtcgagatga cgcgggcacc gcccagcggc atatcgctct tggggccata
    17401 gctctcagtc tggcctgcgc cgtcggcaca caggcggcgc acaatggcgg gcagcgcggc
    17461 gttagagccg atgccgacga ccgcgtccac ctcggggatc tcctggatga tctgttgctt
    17521 gtagcgctcg gccaggcagc cggtgacgat gaccttgagg gccgggttct gctgcttgta
    17581 ctggcaggcc atcaggatat tctcgatggc ctcggtcttg gcggactcga taaatccgca
    17641 agtgttgatg atgatgatgt ccgcctcggc cgggtcgccc acggtggtgt ggccgtcctt
    17701 gatgagggca tggcagaaaa cgtcggcgtc cacctggttt ttcgggcagc cgagagagat
    17761 aatggcaatt ttcattgggt agtcctttta taatcagtat gatttttaaa tatccgggag
    17821 attgtaaggg gcgatgctcg catccgcccc ctacaaatta agacgcagcg aaattactcg
    17881 tcgtcggata agacctcacg tatgaccgcg tggctgaacc cgcgccggac cagagcggcg
    17941 gcgacctggt cgcgcttgcc cgcagccagc ttggcggcgt actgccgctc caccaatgcc
    18001 cgggcagtgg cgagttccgg gttttcgccg tcttcgccct cggcaaacaa ctccttcacc
    18061 acctcagcgg cggtgtcgcg gtcgatacct tttgccgaca aatcgtttaa aatttcacgg
    18121 cgggattttc gcttgcgcag cagctccgct gcacggcggc gggcaaaccc ggcatcgttc
    18181 aacaagccca gctcccccgc ccgcgccacg gcgtaggcgg cgctgtgggc gtcgaactta
    18241 cggcagagct tttggtacag ctcccccgcc gcatggtcgc gcagggccag gtaatccatg
    18301 gccttgtcca gtgcgcggcg ggtttcgctt ttcttgcgca gttcctcaat ttgccactct
    18361 tccaggtcat catcctgatg cagcccggca tcggcaaagg tgttttcatc cagcgagaat
    18421 gcaaattcac cgtcaaaaaa cagcgcaagt ctgcctttct ttgtctcttt gatttgcgtc
    18481 aatcgcagca tgggttattc atcgaccata atatcgaggt ctacctcgct gccggtggtt
    18541 ttggcagcgg gggcggcggg ggcttcaccg gcggcgaccg gcttgacgat ctcgctggga
    18601 tccagcggct tgacagtgcc cttcttgccg gcggccagca gacggtcggc gttggcgcgg
    18661 acgatctttt cgatctcgtc ggagacttcg gggtgatcct tcaggaactg ctttgcgttg
    18721 tcgcggccct ggcccaggcg catttcgccg taattgaacc acgcgccgct cttctgcacg
    18781 acgcccagct tgacgccgag gtcaaggatt tcgccgatct tggagatacc ttcgccgaac
    18841 atgatgtcaa actcggcctc acggaacggg ggtgcaacct tatttttgac gattttagcg
    18901 cgggtgcggt tgccgatgaa tgcgccggtg gagtccttca gaccctcgat acggcgcacg
    18961 tcgatacgca cgctggaata gtatttcagt gcacggccgc cggtggtgac ttcggggttg
    19021 ccatagacga tgccgacttt ttcgcgcagc tggttgatga agatgcagac ggtgttcgtc
    19081 tttccgataa cgccggtcag cttgcgcagg gcctggctca tcaggcgggc ctgcaggccg
    19141 acatggctgt cgcccatctc gccctcaatt tcggcgcggg gcaccatggc ggcaacggag
    19201 tcgacaacga ctgcgtcaat cgcgccggag cgcacaaggg cctcgcaaat ttccatggcc
    19261 tgttcaccgg tgtccggctg gctgaccagc aggctgtcga tatcgacacc caaggcggcg
    19321 gcgtaggtgg ggtcgagggc gtgctcaaca tcgatgaaag cgacctcgcc gcccatcttc
    19381 tgggcctcgg ccagaatttg cagtgccagc gtggttttac cggaagattc tgggccgtag
    19441 acctcaacga tacggccgcg gggcacgccg ccgatgccca gcgccaggtc gaggcccagg
    19501 ctgccggtgg ggatggcgtc gacctgcatc gtgacgttgg cgcccagctt catgacggca
    19561 cccttaccga actgcttttc gatctgcgcc agcgcggttt ccagcgcggc ctttttatcg
    19621 gcggcagggc ctgccgtctt gggggtggta tcttttttag ctgccatgtt gtgtttctcc
    19681 ttttatatgc aaagtaaggt tatgatttgc gtttgcctct attatatacg atttacgaac
    19741 agattacaac attttgttgt gcgttttgtg tcccatagac cggattttca ggcggttttt
    19801 tcaatttttt tgcagcaaaa caacccggtc gttgccgccg tagtccttgc ggcagctgcc
    19861 gttggcccag ccgtggatct cgcccagggc cagcacatcg gcggcctggg catagccaat
    19921 ctccatgacc agccagccgc ccgggcgcag tgcggccttg tagcgggtcg tcagcaagcg
    19981 gtaaaaatcc aggccgtccg cgccgccatc cagcgccatg gcgggttcgt gggccgtctc
    20041 tggcatcaac gcccccattt ccggcccggt caggtagggt gggttagaga tgatgagatc
    20101 agcgcactct tcggacagca acttataata ggtaaagacg tcggcctgca tagtacgcac
    20161 gccgctgtcc gccgtgttgg cggtcaaata gggccaggcg tcagggcttt tttccacgca
    20221 ggtcacgtcc gcgccgggga catggcgggc aatgcccagc cccaggcagc ccgtgcccgc
    20281 gcagaggtcg tagacccgcg gcgacggcac gccttgcagc agctcgatgg ccgtctcgca
    20341 gacgacctcg ctgtcagccc gggggcagag cacgccgggg ccgactttga gcgaaaagtc
    20401 cataaaatcc cactcgccta aaatatattg cagcggctcc cgcgcggcgc ggcggtcggc
    20461 cagcgcggcc aggcgggcgg cctcggcttc ggtagggccg tcgcccaggc gcgggtcacg
    20521 gcgggtcaca aagcggtaaa gttccgccgc gtcaaaccgg gcgtcgggca caccggcggc
    20581 ggcaagccga gcgcaaaggg cctgaaatgc agcgttcacc agcgggcttc ctcctgattc
    20641 ttgggcagaa ccggcgtgac ggcggcaatg gcaacctcga tcattgagtc gtcaggttca
    20701 aaggtcgtca gtcgctgcat ccacagcccg ggctgtgaga cgatcttgga cagcaggctg
    20761 tccgagcggc ccgcccattt cagaatttca tatgaaatac cgaccaaaag tgggaacatg
    20821 cacagcttgt acaccacgcg cagggccgtg ctggtccagg gcagcaccgc atacaaaaag
    20881 atgctgatga taatgaccaa gatcataaaa ctggtgccgc atcggggatg gtgcctgggc
    20941 tgcctgcgca cgttctccac cgtcaggggc aggcccgcct catagcagaa gatagtcttg
    21001 tgttccgccc cgtggtactg g
//
LOCUS       DBMDSQ010000006        18449 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_987_length_18449_cov_2.422498, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000006 DBMDSQ010000000
VERSION     DBMDSQ010000006.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 18449)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 18449)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18449
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_987_length_18449_cov_2.422498"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(249..1904)
                     /locus_tag="ACZPAX_00490"
     CDS             complement(249..1904)
                     /locus_tag="ACZPAX_00490"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005924376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese-dependent inorganic
                     diphosphatase"
                     /protein_id="HIR1112406.1"
                     /translation="MANTMRHINIIGHQNPDTDSICSALAYAWLKNRGSLTGLYEARR
                     AGHVNRETKFVLQHFGVEPPRLCTDVSPQIKDIDIRKQAGIDGEMSLRAAWNLMRDVE
                     IDTLCIVDDENELQGLITIKDIADANMDLFDTAVLAAANTRCTNVLETLEAELIVGNA
                     DAHIDSGVICIGTSPEIMEELVKPGDIVLVSNRYETQMCAIDCGAQAIIVCCGSAVPR
                     TIVARAQEKGCAVLATPYDTYAAARLISTAAPVRHFMRTKELLKFSVNTPIEDAKKVM
                     ASVRHRYFPILDENGKYCGVVSRRNLLNLHRKQLILVDHNERTQAVDGLEQADILEII
                     DHHRIGSLETTGPVYFRNVPVGCTATILYQMYGEQGLTPSREIAGLLLSAILSDTLVF
                     RSPTSTPQDEATAKALAEIAGEDIQIYAEQMFEAGADLTGRTAEDVFSSDFKAFSRGD
                     VKFGVGQSTYMTDKSRAAAEALVEPYLPEASRREGLPLIFYMFTDMKTQSTDLMFYGH
                     NAEEIIRDAFHVEPEGDIAKLPGVVSRKKQLIPPLLAALQARQ"
     gene            2096..3925
                     /gene="typA"
                     /locus_tag="ACZPAX_00495"
     CDS             2096..3925
                     /gene="typA"
                     /locus_tag="ACZPAX_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HIR1112407.1"
                     /translation="MIQENLRNVAIIAHVDHGKTTLVDQMLKQSGAFRANQQVAERVM
                     DSGDIERERGITILAKNCSCEYNGVKINIVDTPGHADFGGEVERVLKMVNGVLLLVDA
                     AEGCMPQTRFVLQKALQQNLSLVIAVNKIDRPDARIAEVIDEILELLMDLGATDEQLD
                     SPMVFCSGRNGTASYDWTDPSSGTDLKPLFDTILKYVQPPEGEPDEPLQMLVSSVDYN
                     DFVGRMGVGRVQNGVIKVGSPIQVCDWHNPDVHMSGRITKLFDFKANGREPIESAQAG
                     DIVAFAGLPDISIGNTICDPSKVQPLPFVKINDPTVEMTFSVNDSPFAGKEGKYVTSR
                     QIRDRLMRELLKDVALKVEDSATNDSFRVMGRGEMHLSILIETMRREGYELQVSPPHV
                     LTHEENGKVMEPMERVVIDVPEVYQGNVMTALGARKAILMNMQMMNSRSRIEFRMPSR
                     GLFGYRSQFLTDTHGEGIMNTIFDGYEEWCGDIQTRSTGSLISFETGDAVTYGLFNAQ
                     QRGTLIVNAGEKVYAGEVVGYTPTGEDITVNVCKTKHLTNTRASGSDDALRLVPVSKF
                     SLEGCLEFLAPDELLEVTPENLRIRKRILDHDLRMKAASKKNK"
     gene            complement(4069..5298)
                     /locus_tag="ACZPAX_00500"
     CDS             complement(4069..5298)
                     /locus_tag="ACZPAX_00500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1112408.1"
                     /translation="MKKLALVFAAALALTACTVAPTETATVPAADPPAEAAATEENAD
                     AEQFPVGELRLCDDRRCDTDTAYYTLYGHVDTTELTGLRLDYADGVQTKVTSLAIADA
                     LYSDLLVTNDNVVRVFLANADGSEFRFKSFYPDGRSEERTASQEITPMVYDEYAAYVL
                     RDNCVARLDWQTGEVTTWDTSIPQIDEILGAVGNKILLTRIVSDMPLPTDPEMYEAVI
                     QNSQREFDLYDIATNTLEKLFAEPRYPEDGGSWKIYRGCRGDILYFAQRKSDGDGYKE
                     ALLGYDLTTGTMQELYAEEATDCVGAFNSPKSFSRGGQLELVFLQSTPDALHIYNIAD
                     GQMYNVPCHDPTLNAAYGIEENYGRPIAVTGDGRVLVIDGYAQRGGYTVDAYGLIDLD
                     DYLAGSTDYQPVQMWAQ"
     gene            complement(5301..6533)
                     /locus_tag="ACZPAX_00505"
     CDS             complement(5301..6533)
                     /locus_tag="ACZPAX_00505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1112409.1"
                     /translation="MKKLALVFAAALALTACTAAPTETTTIPAADPPAEAAATEENAD
                     AEQFPVGELRLTAFDNTDGTALYGGFSSGNNTVWTVLRLDYATGVQSKLCDLTFTDGL
                     NMNCMITMGENEVAFCSDEDDTQHSYIIVRPDGTTDDHTISRAFTPTVYDEYAAYEVN
                     GTSCARLDLNTGEVTDFSAPLLQLASVEGVVDNRLLVTRIVSDTPLPAEGEDEEMREA
                     ILQNSLREYDLYDLATNTVEKLFDEPYYPEDGSSSWKTYEGCRGGTLYFVHTLWDKTT
                     PDNTDNTLLAYDLASGTWQELCTLTDKQGSWCPGFSKNGQLEYVLLWQSADTLTLYNL
                     ADGTTHDVPYQVPAGGQYGSGKNYGWPLDLTGDGRILVTDGYAQQGGYATDGYALVDV
                     DDYLAGSTDYQPVQMWEE"
     gene            6750..8084
                     /locus_tag="ACZPAX_00510"
     CDS             6750..8084
                     /locus_tag="ACZPAX_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010299609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HIR1112410.1"
                     /translation="MTAFTTFVEQFSDIVWNSFLLFLLVGTGIFFTIRLRGVQVRRFG
                     EGFRRVFGGFTLRGKKADAEGMSSFQALTTAIAAQVGTGNITGCATALVSGGPGALFW
                     TWMSAFFGMATIYAEAVLAQHYKTTVNGHVTGGPAYYIRAAFKGKLGKILATVFSVLI
                     ILALGFMGNMVQSNSIGDAFHNAFGVNRLVVGVVVAAIAAFIFLGGVQRIAAVTEKIV
                     PVMAAFYIVGCIVILVMNAKVLPNALKQVFVLAFQPQAMAGGLAGVTVQQAMRFGVAR
                     GLFSNEAGLGSTPHAHALAKVKQPQDQGAVAILGVFIDTFVVLTLTGLVLITSGLIPE
                     GLTGTALTQAAFSQAFGGFGPIFIAVCMFFFAFSTIIGWYFFGQSNFKTLFGEKMLPV
                     YSLIVVVFILVGSTLKVDLVWALADLFNGLMAVPNLLALLALSGVVVAIDRK"
     gene            complement(8397..9524)
                     /gene="nagA"
                     /locus_tag="ACZPAX_00515"
     CDS             complement(8397..9524)
                     /gene="nagA"
                     /locus_tag="ACZPAX_00515"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006875497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HIR1112411.1"
                     /translation="MIIKNAKVYSDGCRFVEKDLIIRNGRIVFGAAAQEGEEIIDAGG
                     AYALPGLVDIHFHGAVGHDFCDADETGLQAIADFEASKGVLAICPATMTFSEEILSGI
                     MDVAAAHKNDHGADLVGINMEGPYISPKKVGAQNPKYVMGADAAMFRRLQARSGGLIK
                     LVDVAPEEPGNLDFIKECRDEVRISIAHTCTSYDTAKAAFAAGATHMTHLYNAMPGIT
                     HREPGPIIAALEEGAEVELITDNVHIHPAMVRFTFNTFGDDHVILIADSMMACGLPDG
                     QYSLGGQAVTVRGPRATLTEQPGTIAGSATCLFDCMKRAVLEMGVPLESAVRAATLNP
                     ARSIGIDADYGSLDAGRIGNVVLVDDKLDILKVIRHGEVLC"
     gene            complement(9544..10272)
                     /gene="nagB"
                     /locus_tag="ACZPAX_00520"
     CDS             complement(9544..10272)
                     /gene="nagB"
                     /locus_tag="ACZPAX_00520"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005936840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HIR1112412.1"
                     /translation="MKIIRAKDYYDMSRKAANIISAQVIMKPNCVLGLATGGTPVGTY
                     KQLVEWYNKGDIDFSEVTTVNLDEYRGLPKEHPESYWSFMHRNLFDHVNIRPEAINLP
                     DGTNLDAAAECARYDAIIHSVGGVDLQLLGIGNDGHIGFNEPNEAFELGTHCVDLKEE
                     TIEANKRFFDGNADLVPKQAYTMGIKTIMQARKVLMVANGKGKADIVKKAFFGPVTPE
                     VPASILQMHPDFILVGDEEALSEI"
     gene            complement(10492..11982)
                     /locus_tag="ACZPAX_00525"
     CDS             complement(10492..11982)
                     /locus_tag="ACZPAX_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015537661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HIR1112413.1"
                     /translation="MMKYLQKLGKALMLPVAALPVCGILMGIGYALAPAVMGAEGPTS
                     GAAYTVGFLLIKAGGALIDNMAWLFAIGAAVGLSDDHDGTAGLAGLVSFLMMQQLLSP
                     AVVGAIRAAVGSTLEEGTVDYIAFSKIAGNSFIGILAAVIGAACYNKFKNTQLPDWLA
                     FFSGKRSVAIVTAVVSIVVSVVLLFVWPVIFGVLVALGNGIAKMDGIGAGIYAFLNRL
                     LIPTGLHHALNNVFWFDTIGLGDLSHYWAGDVTGQNGVNWSLGMYMSGFFPCMMFGIA
                     GAALAMVQTAKNKKAAIGLVVSAAICAFVCGVTEPFEFGFMFLCFPLYVVYAALYGIF
                     TIITYYTGFRAGFCFSAGATDLIFSASLPAHANTWMIIPLGIAAFVVFYLVFKFAIVK
                     FDLKTPGREDEDAEAAEANITLANNDFTAIASGVLAAVGGKGNVANVDYCATRLRFEV
                     KDSTAVNEKAVKAAGAAGVIRPSKTACQVVIGPKVQFVYDELKKML"
     gene            12390..13892
                     /gene="glpK"
                     /locus_tag="ACZPAX_00530"
     CDS             12390..13892
                     /gene="glpK"
                     /locus_tag="ACZPAX_00530"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014355664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HIR1112414.1"
                     /translation="MANQKYIMALDAGTTSNRCILFNARGEMCSVAQKEFTQYFPKPG
                     WVEHDANEIWTTQLGVALSAMNQIGASAEDIAAIGITNQRETTIVWDRDTGEPVCHAI
                     VWQCRRTSEYCDELKARGLTEKFREKTGLVIDAYFSATKLKWILDNVPGVRARAERGD
                     LLFGTVETWLIWKLTCGKIHVTDYSNASRTMLFNIHTLDWDDEILQILDIPRCMLPKP
                     VPNSEFYEYADPMHFGGEIKIAGAAGDQQAALFGQTCFTKGEAKSTFGTGGFLLMNTG
                     EKPVFSHNGLVTTVAWGLDGKVNYALEGSIFVAGAAIQWLRDELHLLEDARDSEYMAQ
                     KVKDTNGCYVVPAFTGLGAPHWDQYARGAIVGLTRGVNKNHIIRATLDSLCYQINDVL
                     TAMQADAGIPLTALKVDGGACANNYLMQTMADISALPVKRPCCVETTALGAAYLAGLA
                     VGYWQSTDDVLKNWSVDREFVPALTEAERTRRIQGWNKAVRCSYGWAKED"
     gene            14211..15647
                     /locus_tag="ACZPAX_00535"
     CDS             14211..15647
                     /locus_tag="ACZPAX_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004603121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR1112415.1"
                     /translation="MHDIIIIGAGVSGCACARELSRYNADILVLEKEEDVCCGTSKAN
                     SAIVHAGYDAANGSLMAQLNVEGSRRMPALAKELDFAYDQCGSLVVCLSEDDRPALQK
                     LYENGVANGVEGLRIVERDELVTMEPNIADTAVAALWAPTGGIVCPFGLTYALAENAA
                     KNGVRFRFDTKVTGLHPLPGGGWQVDTDRGSCQARCIVNAAGVYADTLHNLADAAHPM
                     KITARRGDYFLLDHTAGQHVRHTVFQLPGKYGKGVLVTPTVHGNLLVGPTAIDVDDKE
                     ATATTAAGLDEVRTKSGLAVKDIPLRQTITSFAGLRAHEARHDFFIGEIAPGFVDCAA
                     IESPGLSSAPAIGAMVAGIVRDSLQLKENPTFDPTRKGILDPKALDFAARAELIKQNP
                     AYGQIICRCESVTEGEIIDAIHRTPGARSLDGVKRRTRAGMGRCQAGFCSPRVMEILA
                     RELGVAQSEITKSGGDSKIIVGTNKDAF"
     gene            15664..16917
                     /locus_tag="ACZPAX_00540"
     CDS             15664..16917
                     /locus_tag="ACZPAX_00540"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013378410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR1112416.1"
                     /translation="MKNVDLVIIGGGPAGLAAAVAARKNGVQDILILERDSELGGILN
                     QCIHNGFGLHTFKEELTGPEYAARFIAQVQELGIEYKLNTMVLDLAADKTVTAMNKSD
                     GLFQLHPKAVILAMGCRERPRGALNIPGYRPAGIFTAGTAQRLVNMEGYLPGRRVVIL
                     GSGDIGLIMARRMTLEGAKVLCVAELMPYSGGLKRNIVQCLDDFDIPLKLSHTVVDIQ
                     GKERVTGITIAQVENGKPIPGTEEHYDCDTLLLSCGLLPENELSRAAGVALSPITGGP
                     SVNESLETNIPGVFAAGNVLHVHDLVDYVSEEAGAAGKHAAAYILKSEHAAGRTLPVH
                     CENGVRYTVPTAIRPDCAGDTVTLRFRVGGVYKNKKIAVYLGDTCIFSRRRPVLAPGE
                     METVRLKSDLLRGAGDAVTVTLEEG"
     gene            16920..17288
                     /locus_tag="ACZPAX_00545"
     CDS             16920..17288
                     /locus_tag="ACZPAX_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008399373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1667 domain-containing protein"
                     /protein_id="HIR1112417.1"
                     /translation="MEKRELTCIGCPMGCALTVELDGGAVVSVRGNTCPRGDAYARKE
                     VTAPTRIVTTTVRVTDGALAAVSCKTRGDIPKGKIFDIIRALKAVEVAAPVVIGQVIL
                     PNAADTGVDVIATKNVEKRG"
     gene            complement(17510..>18449)
                     /locus_tag="ACZPAX_00550"
     CDS             complement(17510..>18449)
                     /locus_tag="ACZPAX_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015536714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HIR1112418.1"
                     /translation="ADTIVFDKTGTLTHATPTVVQVVPFGTRTEDEVLQIAACLEEHY
                     PHSMANAVVQAAAAKGIRHDEMHSEVQYVVAHGIASQIGGEKAVIGSQHFVFEDEGCC
                     IPTAECDKFDALPPEYSHLYLAIGGVLAGVICIADPLREEAAEVLRQLRSLGIRKAVM
                     MTGDNDRTASVIANQVGVDRYYSEVLPEDKANFVEAEKAAGHTVIMLGDGINDSPALS
                     AADVGIAISDGAAIAREIADITIAADSLRELVILKSIANGLQKRTKANYRFIMSFNSS
                     LIVLGAMGILPPATSALLHNASTLGVSLKSMTNLLG"
BASE COUNT         3697 a         5376 c         5692 g         3684 t
ORIGIN      
        1 ataagaagtt tatcgccgcg ggggttcggg ggaacacccg actcaaaagt ggaggaaggt
       61 tcggcggcgt taagaacgga cagtaatact gcgtccgttt agccgccgaa gcttccgact
      121 tccgctttgg gggtctcggg ggcgtgcagc ccccgaggcg cgggttcgct gcgtcgcaac
      181 agcgagactt ttctttgtta ctttcttttg gtcacaaaag aaagtaagac atcccgggag
      241 actgccgttt actgccgcgc ctgcagcgcg gccagcagcg ggggaatcag ctgttttttg
      301 cggctgacga cgccgggcag cttggcaata tcgccttccg gctcaacatg gaacgcatcc
      361 cggatgatct cctcggcgtt gtggccgtag aacatcaaat ccgtactctg ggtcttcatg
      421 tcagtgaaca tatagaaaat cagcggcaga ccctcgcggc ggctcgcttc gggcagatac
      481 ggctccacca gcgcctcagc ggcggcgcgg gatttgtcgg tcatgtaggt ggactgaccg
      541 acgccgaact tgacgtcgcc gcggctgaac gccttgaagt ccgagctgaa cacatcctcg
      601 gctgtgcggc cggtcaggtc cgcgcccgct tcaaacatct gctcggcgta gatctggata
      661 tcctcgcccg caatctcggc cagcgccttg gcggtggcct cgtcctgcgg ggtggacgta
      721 ggcgagcgga acaccagtgt gtcggacaga atggccgaca gcagcaggcc cgcgatctcg
      781 cggctgggcg tcaggccctg ctcgccgtac atctggtaca aaatcgttgc cgtgcagccc
      841 accggcacat tgcggaaata gaccggcccg gtcgtttcca ggctgccgat gcggtgatgg
      901 tcaatgatct ccaaaatatc cgcctgttcc agaccgtcca cggcctgggt gcgttcgttg
      961 tggtcaacca aaatcagctg cttgcggtgc aggttcaaga ggttacggcg gctgacgacg
     1021 ccgcagtatt tgccgttctc gtccagaatc gggaagtagc ggtggcgcac gctggccatg
     1081 acctttttcg cgtcctcaat cggcgtattg acgctgaatt tcagcagctc tttcgtgcgc
     1141 ataaagtggc gcaccggcgc ggcggtggag atgagccgcg cggcggcgta ggtgtcgtag
     1201 ggggtcgcca gcacggcgca gcccttctcc tgggcgcggg ccacgatggt gcggggcacc
     1261 gccgagccgc agcagacgat gatggcctgt gcgccgcagt cgatggcgca catctgcgtc
     1321 tcgtaccggt tggagacaag cacgatgtcg ccgggcttga ccagttcttc catgatttcc
     1381 gggctggtgc cgatgcagat gacgccgctg tcaatgtggg cgtcggcgtt gccgacgatg
     1441 agctccgctt ccaacgtctc caacacattt gtgcagcggg tgttggcggc ggccagcacg
     1501 gccgtgtcaa acagatccat gtttgcgtcg gcgatgtctt tgatggtgat aaggccttgc
     1561 agctcgttct cgtcatcgac gatgcagagc gtgtcgattt ccacatcgcg catcaggttc
     1621 caggcggcgc gcaggctcat ttcgccgtca atgccggcct gcttgcggat gtcgatatct
     1681 ttaatttgcg ggctgacatc ggtacacagg cgcggcggct cgacgccgaa atgctgcaaa
     1741 acaaatttcg tctcgcgatt gacgtgcccg gcgcggcggg cctcatacag gccggtcagg
     1801 ctgccgcggt ttttcagcca cgcataggcc agtgcggagc agatggagtc cgtgtccggg
     1861 ttctggtgac caatgatatt gatgtggcgc atcgtatttg ccatagtggt tcccctcatt
     1921 gcatgattag tgtaaaatat aactataata ttctgtatta tagcacactt ttttgcgttt
     1981 tgcgattctt gacaaattga acataatagc gtattataaa atagtatgtg gatattgggg
     2041 ttatacccct gcaaatatca gtatatcggc atttttaatt tgagagggaa cgtatatgat
     2101 tcaggaaaat ctccgcaacg ttgcaatcat tgcgcacgtt gaccacggca aaacgacgct
     2161 ggtcgaccag atgctgaagc agtccggtgc tttccgtgcg aaccagcagg tcgccgagcg
     2221 cgtcatggac tccggcgata tcgaacgcga gcgcggcatc acgatccttg ccaagaactg
     2281 ctcctgcgag tacaacggcg ttaagatcaa catcgtcgac accccgggcc acgcagactt
     2341 cggcggcgag gtcgagcgcg ttttgaagat ggtcaacggc gttctgctgc tggtcgatgc
     2401 agccgagggc tgcatgcccc agacccgctt tgttctgcag aaggctttgc agcaaaacct
     2461 gtctctcgtc atcgcggtca acaagatcga ccgccccgac gcccgcattg cggaagtcat
     2521 cgacgagatt ctggagctgc tgatggacct gggtgctacc gacgagcagc tggacagccc
     2581 tatggtcttc tgctccggcc gtaacggcac ggccagctat gactggaccg atccttcctc
     2641 cggcacggac ctcaagcccc tgtttgacac catcctgaaa tatgtgcagc cgccggaggg
     2701 tgagcctgac gagccgctgc agatgctggt ttccagcgtt gactacaacg atttcgtcgg
     2761 ccgcatgggc gttggccgcg tgcagaacgg cgttatcaag gtcggcagcc ccatccaggt
     2821 ctgcgactgg cacaaccccg atgtgcatat gagcggccgc atcaccaagc tgttcgactt
     2881 caaggccaat ggccgtgagc cgatcgagag cgcccaggca ggcgatatcg tcgccttcgc
     2941 aggtctgccc gatatctcca tcggcaacac catctgcgac cccagcaagg tgcagcccct
     3001 gcccttcgtc aagatcaacg accccacggt cgagatgacc ttctcggtca acgattcccc
     3061 gttcgccggc aaggagggca agtacgtcac ctcccgccag atccgtgacc gcctgatgcg
     3121 cgagctgctg aaggatgtcg ccctgaaggt cgaggattcc gccaccaacg attccttccg
     3181 cgtcatgggc cgcggcgaaa tgcacctgtc catcctgatc gagacgatgc gccgcgaagg
     3241 ctatgagctg caggtcagcc ccccgcacgt tctgactcac gaggagaacg gcaaggtcat
     3301 ggagcctatg gagcgcgttg tcatcgacgt gcccgaggtc tatcagggca acgtcatgac
     3361 cgctctgggt gcccgcaagg ccatcctgat gaatatgcag atgatgaaca gccgttcccg
     3421 cattgagttc cgtatgccca gccgcggcct gttcggctat cgcagccagt tcctgaccga
     3481 tacccacggt gagggcatca tgaacacgat cttcgacggt tacgaggaat ggtgcggcga
     3541 tatccagacc cgctccaccg gctccctcat cagctttgag accggcgacg ccgtcaccta
     3601 cggcctgttc aacgcccagc agcgcggcac cctgatcgtc aacgccggcg agaaggtcta
     3661 cgcgggcgag gtcgtcggct acaccccgac cggcgaggat atcacggtca acgtctgcaa
     3721 gacgaagcac ctgaccaaca cccgcgcctc cggcagcgac gacgccctgc gtctggtgcc
     3781 ggtttccaag ttcagcctgg agggctgcct ggaattcctg gctcccgacg agctgctgga
     3841 agttaccccc gagaacctgc gcatccgcaa gcgcattctg gaccacgacc tgcgcatgaa
     3901 ggccgccagc aagaagaata agtaattttg cacgatacag caagagccgc ccccgagtgg
     3961 agcggccctt gcttttttat ttacgattct gcggtaaaac attgccgccc gcgcgtgtac
     4021 ggaaaacggt cgtttatggg aagtccacgg gcggcatata tgccgcccct actgtgccca
     4081 catctgcacg ggttggtagt cggtgctgcc tgccagataa tcatccaggt cgatcagtcc
     4141 ataggcatcc accgtgtagc cgccgcgttg ggcgtaaccg tcaatcacaa gcacacggcc
     4201 atccccggtc acggcgatgg ggcggccgta gttttcctcg ataccatagg cggcgttcag
     4261 cgtcgggtcg tggcagggta cgttgtacat ctggccgtcg gcgatgttat agatatgcag
     4321 tgcgtccggc gtgctttgca ggaacaccaa ttccaactgc ccgccgcggc taaagctctt
     4381 cgggctgtta aaggcaccca cacaatcggt ggcttcctct gcgtacaact cctgcatggt
     4441 gcctgtcgtg aggtcgtacc ccagcaaagc ctctttgtag ccgtcgccat ccgattttct
     4501 ctgcgcaaaa tacagtatat ctccgcggca gccccggtaa attttccagc tgccgccgtc
     4561 ctccggatag cgcggctcgg caaatagctt ttccagcgta tttgttgcta tatcgtataa
     4621 atcgaattct cgctgggagt tctgtatcac agcctcatac atttccggat ccgtaggcag
     4681 cggcatatca gagacgatgc gggtcaacaa tatcttgttc ccaaccgcac cgagtatctc
     4741 atctatctgc ggaatcgatg tgtcccacgt cgtaacttct ccggtttgcc agtccagccg
     4801 ggcgacacag ttgtcgcgca ggacataggc cgcgtactcg tcataaacca ttggcgtaat
     4861 ttcctggctg gcagtccgtt cttcactgcg gccatccggg taaaaactct taaagcgaaa
     4921 ctctgatccg tccgcattcg ccaaaaacac gcgaactaca ttatcgtttg tgacaagcaa
     4981 atcggagtac agggcatcgg caatagcaag cgacgttacc tttgtctgca cgccgtcggc
     5041 atagtccagc cttagccccg taagctcagt cgtgtctacg tggccataca gcgtgtaata
     5101 ggcagtgtcc gtgtcacacc gccggtcatc acacagccgc agttccccca ccgggaactg
     5161 ctccgcgtcc gcattttcct cggttgccgc cgcctcggcg ggcgggtcgg cggcagggac
     5221 ggttgctgtc tcggtcggcg caacggtgca ggcggtcagg gccagcgcag ccgcaaatac
     5281 aagagcaagt tttttcatga ctattcctcc cacatctgca caggttggta gtcggtgctg
     5341 cccgcaaggt aatcatcaac atcaaccagc gcgtatccat ccgtcgcata accgccctgc
     5401 tgggcgtagc cgtcggtcac tagaatgcgg ccgtcgcccg tcaggtctaa cggccagccg
     5461 tagtttttgc cgcttccgta ttgtcctccc gcaggtactt ggtacggtac atcgtgggtc
     5521 gtgccatcgg ccaggttgta cagcgtcagg gtgtccgccg actgccaaag cagcacatat
     5581 tccaactgtc cgtttttgga aaacccaggg caccagctgc cttgcttgtc ggtcagtgtg
     5641 catagctcct gccaggtacc gctggccagg tcgtaggcta acagtgtgtt gtcggtgttg
     5701 tctggggtgg ttttgtccca aagcgtatgc acaaaataca acgtgccgcc ccggcagcct
     5761 tcgtaagttt tccaggatga tgacccatcc tcgggatagt acggttcatc aaatagtttt
     5821 tcaaccgtgt ttgttgccaa gtcgtacaga tcgtactccc gcagggaatt ttgcaggata
     5881 gcttcgcgca tttcctcgtc ctcgccctcg gcaggaaggg gcgtatctga cacaatgcgg
     5941 gtgacaagca gccgattgtc caccacgccc tccaccgagg ccagctgcaa aagcggtgcg
     6001 ctaaagtccg ttacttcgcc ggtattcaag tccagccggg cgcagctagt gccgttgact
     6061 tcgtaggcgg catactcgtc atagacggtg ggggtgaacg cccggctgat ggtgtggtcg
     6121 tccgttgtgc cgtccgggcg gacaattatg tagctgtgct gcgtgtcatc ctcgtctgaa
     6181 cagaacgcga cctcgttttc gcccatagtg atcatgcagt tcatattgag gccatccgtg
     6241 aaggtaaggt cgcatagctt actctgcacg ccggtggcgt agtccagccg cagaaccgtc
     6301 cacactgtgt tgttcccgct gctgaaaccg ccgtacaagg ccgtgccatc cgtgttatca
     6361 aaggcagtca gccgcagctc ccccaccggg aactgctccg cgtccgcatt ttcctcggtt
     6421 gccgctgcct cggcgggcgg gtcggcggca gggatggttg ttgtctcggt cggcgcagcg
     6481 gtgcaggcgg tcagggccag cgcagccgca aatacaagag caagtttttt catggtgatt
     6541 cccctttctg gtttgatttg cgggtagtat accatggggt tgtgtcctac ttgttacaca
     6601 gaagggcgat tttggcagta tttacaaatt ttttttgtta aactgtgcgc cgaataaata
     6661 cacttgactt tacacaaaaa tcatagtata gtaataaggc acccgcattg cggcggggta
     6721 ttgggatgtg aaagaaatag ggggtcaata tgacggcatt taccacgttt gttgagcagt
     6781 tcagcgacat tgtctggaac tcgtttctgc tgtttttgct cgtaggcaca ggcattttct
     6841 ttacgattcg gctgcggggc gtgcaggtgc gccgctttgg cgagggcttc cgccgcgtgt
     6901 tcggcgggtt cacgctgcgc ggcaaaaagg cggacgccga gggcatgagc agctttcagg
     6961 cgctgacgac ggccattgcg gcgcaggtgg gcaccggcaa catcaccggc tgcgccacgg
     7021 cgctggtcag cggcggcccg ggtgcgctgt tctggacctg gatgtcagcg ttcttcggca
     7081 tggcgaccat ctatgccgag gccgtgctgg cgcagcacta caagacgacc gtgaacggcc
     7141 atgtgaccgg cggcccggcg tactacatcc gggcggcatt caagggcaag ctcggcaaga
     7201 ttctggcgac cgtgttctcg gtacttatca ttctggcgct gggcttcatg ggcaacatgg
     7261 tccagtctaa ctccatcggc gacgcattcc acaacgcctt tggcgtgaac cggctggtgg
     7321 tgggcgtcgt tgtggcggcc attgcggcgt tcatcttcct gggcggcgtg caaagaattg
     7381 ccgctgtaac ggaaaaaatc gtacctgtca tggcggcgtt ttacattgtg ggctgcatcg
     7441 ttatcctggt catgaacgcg aaggtcctgc ccaacgcatt gaagcaggtg tttgtgctgg
     7501 cattccagcc gcaggcgatg gcgggcggcc tggccggtgt gactgtgcag caggccatgc
     7561 gttttggcgt ggcccgcggc ctgttctcca acgaggcggg ccttggctcg acgccgcatg
     7621 cccacgcgct ggcaaaggtc aagcagccgc aggatcaggg cgcagtggca attctgggtg
     7681 tgtttatcga cacgttcgtt gtgctgacgc tgaccggcct ggtgctcatc acgtcggggc
     7741 tgatccctga gggactgacc ggcacggcgt tgacccaggc tgcgttcagc caggcgttcg
     7801 gcgggtttgg gccgattttc attgcggtgt gtatgttctt cttcgcgttt tcgacgatca
     7861 ttggctggta tttcttcggc cagtcgaact ttaaaacgct gttcggtgag aagatgctgc
     7921 cggtgtacag ccttatcgtg gtcgtattta ttttggttgg ctccacgctg aaagtagatt
     7981 tggtgtgggc gctggccgac ctgttcaatg gcctgatggc tgtgcccaac ctgctggccc
     8041 tgctggcgct gtcgggtgtg gtcgtggcga tcgaccggaa gtgagaacgg atggatagat
     8101 aggaataaag caaaaaatcc tgccgatgaa agtgtcggca ggattttttg tatgatggaa
     8161 gggatggtaa aggcaaacgg cccggggcgt cgaggacgcc gcccccccta caactaaagt
     8221 ggtgagttgc agcaagggca ctgtgcggcg gccagagggc tggccgccct acaactctac
     8281 atactttagt ttttccctaa agtgcgcggg ggaacacccg acttcctctt tggggttcct
     8341 aggggcgtgg gtacgctccg tcgaaaaata tctcccggga gactgccgtt tacactttag
     8401 cagagcacct cgccgtggcg aatcaccttc aggatatcca gcttatcgtc aacgaggaca
     8461 acattgccga tccgcccggc atccaggctg ccgtaatcgg catcgatgcc gatgctgcgg
     8521 gccgggttca gggtagcggc gcggacggca ctctcgaggg ggacgcccat ctccagcacc
     8581 gcacgcttca tgcagtcgaa caggcaggtc gcactgcccg caatcgtgcc gggctgctcg
     8641 gtcagggtcg cgcggggacc gcgcaccgtg acagcctggc cgcccaggct gtactggccg
     8701 tcgggcaggc cgcaggccat catgctgtcg gcaatcagga tcacatggtc gtcgccaaac
     8761 gtgttaaagg taaaccgcac catggccggg tggatgtgta cgttgtcggt gatcagctcg
     8821 acctcggcac cttcttccag cgcggcaatg atggggcccg gctcacggtg ggtgatgccc
     8881 ggcatcgcgt tgtacaggtg ggtcatgtgg gtggcaccgg cggcaaacgc ggccttggcc
     8941 gtgtcgtaac tcgtgcaggt gtgggcgatg gaaatacgca cctcgtcgcg gcattccttg
     9001 atgaaatcca ggttgcccgg ctcctcgggg gctacgtcca ccagcttgat cagcccgccg
     9061 ctgcgcgcct gcaaacggcg gaacatcgca gcgtccgcgc ccatgacgta cttcgggttc
     9121 tgggcaccga ccttcttcgg gctgatgtag gggccttcca tgttgatgcc caccaaatct
     9181 gcaccgtggt cgttcttgtg ggcggcggcc acatccatga tgccgctcag gatctcctcg
     9241 ctgaaagtca tcgtggcggg gcagatagcg agaacgccct tgctggcctc aaagtcggcg
     9301 atggcctgca ggcctgtctc gtcagcgtcg cagaaatcat ggccgaccgc gccgtgaaag
     9361 tggatatcca caagacccgg cagcgcgtag gcaccgcctg cgtcgatgat ttcctcgccc
     9421 tcctgggccg ccgcgccaaa cacgatacgg ccgttacgga tgatgaggtc tttttccaca
     9481 aagcggcagc cgtcgctgta cacttttgca tttttgatga tcatagcagt attcctccgc
     9541 tgatcagatc tcgctcaagg cttcctcgtc accgaccagg atgaagtcgg ggtgcatctg
     9601 caggatgctg gcggggacct ccggcgtgac agggccgaag aacgccttct tgacgatgtc
     9661 ggccttgccc ttgccgttgg cgaccatcag gaccttgcgg gcctgcatga tggtcttgat
     9721 gcccatggta taggcctgct tggggaccag gtcggcgttg ccgtcgaaga agcgcttgtt
     9781 ggcctcaatg gtctcctctt tcaggtcaac gcagtgggtg cccagctcga aggcctcgtt
     9841 cggctcgttg aagccgatgt ggccgtcgtt gccgatgccc agcagctgca ggtcaacgcc
     9901 gccgacactg tggatgatgg cgtcatagcg ggcacactcg gccgcagcgt cgaggttcgt
     9961 gccatcgggc aggttgatgg cctcggggcg gatgttcaca tggtcaaaca ggttgcggtg
    10021 catgaagctc caatagctct cggggtgctc cttcggcagg ccgcggtact cgtccaggtt
    10081 gacggtcgtg acctcggaga agtcgatatc gcccttgttg taccactcga ccagctgctt
    10141 gtaggtgccc acgggggtgc cgccggtagc caggcccagc acgcagttgg gcttcatgat
    10201 gacctgggcc gaaatgatgt tggcggcctt gcgggacata tcgtagtaat cttttgcgcg
    10261 gatgatcttc ataagacaat acctcctgtt ttttcggggc gcgttgcccc ggttctccta
    10321 tcttcattat accacgctgc gggtgggata ctataagcag aaattgcttt ttatcccaaa
    10381 attccgcagc agtttttgct atggtaaaaa taaaagccgc gggtgggctt gacgcccgcc
    10441 cgcggcggat ttgataaagg gaaccagacc tttacctggc agtgctgatt tttacagcat
    10501 cttcttcagc tcgtcgtaga caaactgaac cttgggtccg atgacgacct ggcaagcggt
    10561 cttgctgggg cggataacac cggcagcacc ggcagccttg acggccttct cgttgacagc
    10621 agtgctgtcc ttgacctcaa agcgcagacg agtggcgcag tagtcaacgt tggcaacgtt
    10681 gcccttgccg ccgacagcag ccagaacgcc ggaggcgatg gcggtgaagt cgttgttggc
    10741 gagggtgatg ttggcctcag cagcctcggc atcctcgtcc tcacggccgg gagtcttcag
    10801 gtcgaacttg acgatagcga acttgaagac caggtagaag acaacgaaag cggcgatgcc
    10861 cagagggatg atcatccagg tgttagcatg tgcgggcagg ctggcggaga agatcaggtc
    10921 agtagcaccg gcggagaagc agaagcctgc gcggaagccg gtgtagtagg tgatgatggt
    10981 gaagatgccg tacagagcag catagacaac atacaggggg aagcagagga acatgaagcc
    11041 gaactcgaac ggctcggtaa cgccgcagac gaaggcgcag atagcagcgg aaacaaccag
    11101 accgatagca gccttcttgt tcttggcagt ctggaccatg gccagagcag caccggcaat
    11161 gccgaacatc atgcagggga agaagccgga catgtacatg ccgaggctcc agttgacacc
    11221 gttctggccg gtgacgtcgc cagcccagta gtggctcagg tcgcccagac cgatggtgtc
    11281 gaaccagaac acgttgttca gggcgtgatg cagaccggtg gggatcagca gacggttcag
    11341 gaaagcgtag atgccggcgc cgatgccgtc catcttggca atgccgttgc cgagggcaac
    11401 cagaacgccg aagatgacag gccagacgaa cagcagaaca acggaaacca cgatagaaac
    11461 aacggcggtc acgatagcaa cgctgcgctt gccgctgaag aatgccagcc agtcaggcag
    11521 ctgggtgttc ttgaacttgt tgtagcaggc agcaccgatg acggcggcca ggataccgat
    11581 gaaggagttg ccggcgatct tgctgaaagc aatgtaatcg acggtgcctt cttccagggt
    11641 ggaaccaaca gcagcgcgga tggcgcccac aacagcgggg ctgagcagct gctgcatcat
    11701 caggaagctg accagaccgg ccagacctgc ggtaccgtca tggtcatcag acagaccgac
    11761 ggcagcgccg atggcgaaca gccatgccat gttgtcgatc agggcgccgc cggccttgat
    11821 cagcaggaaa cctacggtgt aagcagcacc ggaggtcggg ccctcggcac ccataacggc
    11881 gggagccaga gcgtaaccaa tgcccatcag gataccacag acgggcagag cagcaacggg
    11941 aagcataaga gctttgccga gcttctgcag atacttcatc ataaaagcat tcctccaaaa
    12001 attgtgagtg ggtctttccc aactcggtgc ggggccgcct ggttctgcga acagcgcact
    12061 ttgttaatct catcttaaca cattcttcac aaaaaagaaa gcgtttttcg cagaatcagc
    12121 gaaaagctga attttcgacg gattctttgt gcataattca aacagaccgt tgatttttgc
    12181 ccaaaaatgt taaaaaatca atgattatta agaaagcgat tttcatagca taggcgttca
    12241 gagggaagat ttttcacttt tggtgtcgcc aatgccaaaa atctgcacaa atatttggta
    12301 catttgccac gcgcataaaa gatgaacatc ctgtataata aaggcaaata tcggttttcc
    12361 attattataa aggataaagg agaattgcga tggctaacca aaagtacatt atggcgctgg
    12421 acgccggtac gacgtcgaac cgctgcatcc tctttaacgc ccggggcgaa atgtgcagtg
    12481 tggcacaaaa ggaatttacc cagtacttcc caaaaccggg ctgggtcgag catgacgcca
    12541 acgaaatctg gaccacccag ctcggcgtgg cgctgtcggc catgaaccag atcggcgcgt
    12601 cggcggagga catcgccgcc atcggcatca cgaaccagcg cgagacgacc atcgtctggg
    12661 atcgtgacac cggcgagccg gtctgccacg ccattgtctg gcagtgccgc cgcaccagcg
    12721 aatactgtga cgagctgaaa gcccgcggcc tgaccgaaaa gttccgcgaa aagaccggcc
    12781 ttgtcattga tgcctacttc tcggccacaa agctaaagtg gatactcgat aacgtccccg
    12841 gcgtgcgtgc gcgggccgag cggggcgatt tgctattcgg cacggtggag acctggctga
    12901 tctggaagct gacctgcggc aagatccacg tgaccgacta ctccaacgcc agccggacga
    12961 tgctgttcaa catccacacg ctggactggg acgacgagat tctgcaaatc ctcgatatac
    13021 cgcgctgtat gctgccgaag cctgtgccca acagcgaatt ttacgagtat gccgacccga
    13081 tgcactttgg cggcgagatc aaaattgcgg gcgcggcggg tgaccagcag gccgcactgt
    13141 tcggccagac ctgctttacc aagggcgagg cgaaaagcac cttcggcacc ggcggctttc
    13201 tactgatgaa caccggggaa aagccggtat tctcccacaa cggactggtc acgacggtgg
    13261 cgtggggact ggacggcaag gtaaattacg cgctggaagg ttctattttt gtggctggcg
    13321 cggccatcca gtggctgcgg gacgagctgc atctgctgga ggacgcccgc gacagcgagt
    13381 acatggccca gaaggtcaag gacacgaacg gctgttacgt tgtgcccgcg ttcaccggcc
    13441 tgggcgcacc ccactgggac cagtacgccc gcggggccat cgtggggctg acgcgcggcg
    13501 tgaacaaaaa ccacatcatc cgcgcgacac tggacagcct gtgctaccaa atcaacgacg
    13561 tgctgaccgc gatgcaggcg gatgccggca ttccgctgac agcgctgaag gtggacggcg
    13621 gcgcgtgcgc gaacaattat ttgatgcaga cgatggcgga catcagcgcc ctgccggtca
    13681 agcggccctg ctgcgtggag accacggcgc tgggcgcggc ctatctggcc gggctggcgg
    13741 tggggtactg gcagtccaca gacgacgtac tgaaaaactg gtcggtggat cgcgaatttg
    13801 tgcccgccct gaccgaagcc gagcgcacca ggcgcatcca gggctggaat aaggccgtgc
    13861 gctgcagcta cggctgggcg aaggaggatt gacccaaagc cttcctctta ggggggaggc
    13921 ggccccgcag ggccggatga ggggagaact tacggaagag gtcttttaaa aacgtgcctc
    13981 ccggtagggg ccggacatgt ccggccctct gccaaaaggc aaaattacgc ttgcggaaag
    14041 gcctgacctg cggcagtggc aaggcccccg ggcggatatg gaatccgccc ctacgctgta
    14101 ggggtcgatg cccgcatcga cccggagggc ttccagcacc gcacactctc tgcgggcgac
    14161 caatggtcgc ccctacatgc tctgcaaaca aacaacacga ggtacaatat atgcacgata
    14221 tcatcatcat tggcgcgggc gtgtccgggt gtgcctgcgc acgggaactt tcgcgctata
    14281 acgcggatat tctggtgctg gagaaagagg aggacgtctg ctgcggcacg tccaaggcca
    14341 acagtgccat cgttcacgcc gggtacgacg ctgcaaacgg cagcctgatg gcgcagctca
    14401 acgtcgaggg cagccgccgt atgcctgccc ttgccaagga gctggacttt gcctacgacc
    14461 agtgcggcag tcttgtcgtc tgcctgagtg aggacgaccg ccccgcgctg caaaaactct
    14521 acgagaacgg cgtcgccaac ggcgtggaag ggctgcgcat cgtcgagcgc gacgagttgg
    14581 tcacgatgga accgaacatt gccgacactg ccgtcgccgc cctgtgggca cccaccggcg
    14641 gcatcgtctg cccctttggc ctgacctacg ccctggccga aaacgcagcc aaaaacggcg
    14701 tgcggttccg gtttgacacc aaagtcaccg gtctgcaccc actgcccggg ggcggttggc
    14761 aggtcgatac cgaccgcggc agctgccagg cgcgctgcat cgtcaatgcg gcgggcgtct
    14821 acgccgacac gctgcacaac ctggccgacg ctgcccaccc gatgaaaatt accgcgcggc
    14881 gcggggatta cttcctgttg gaccacacgg ccggacaaca cgtgcggcac acggtgttcc
    14941 agctgccggg caagtacggc aagggcgtgc tggtcacgcc cacggtccac ggcaacctgc
    15001 tggtcggccc cacggccatc gatgtggacg acaaggaggc taccgccaca acggcggcgg
    15061 gcctggatga ggtgcgcaca aaatccgggc tggcggtcaa ggatatcccg ctgcgccaga
    15121 ccatcacgag ctttgcgggc ctgcgcgccc acgaggcacg gcatgacttt ttcatcggtg
    15181 agatcgcccc cggctttgtg gactgcgcgg ccatcgagtc cccgggcctg tccagcgccc
    15241 cggccatcgg cgcgatggtg gcgggcatcg tgcgggacag cttgcagcta aaggaaaacc
    15301 cgacttttga cccgacacgc aagggcattc tggaccccaa ggcgctggat tttgccgcgc
    15361 gggccgaact tattaaacaa aacccggcct acggccaaat catctgccga tgcgagagcg
    15421 tgaccgaggg cgaaatcatc gacgccatcc accgcacgcc cggcgcacgc agcctggacg
    15481 gtgtcaaacg gcgcacccgc gcgggcatgg gtcgctgcca ggcgggcttc tgcagccccc
    15541 gcgtcatgga gatattggcg cgggagctgg gcgtggccca aagtgaaatc acaaaatccg
    15601 gcggcgactc caaaatcatc gtcggcacaa ataaagatgc gttttgaaag gggggctgcg
    15661 gcaatgaaaa atgtagattt ggtcatcatc ggcggcggcc ctgccgggct ggcggcagcc
    15721 gtggcggcac gcaaaaacgg cgtgcaggac attttgattc tggagcgcga cagcgagttg
    15781 ggcggcatcc tgaaccagtg catccacaac ggttttgggc tgcatacctt caaagaagaa
    15841 ttgaccggcc ccgagtacgc cgcacggttc atcgcgcagg tgcaagagct gggcatcgaa
    15901 tataagctca acaccatggt gctggatctg gcggcggaca agaccgtgac ggccatgaac
    15961 aaatcggacg gcctgttcca gctgcacccc aaggccgtga ttctggccat gggctgccgt
    16021 gagcgcccgc gcggtgcgct gaacatcccg ggctaccgcc cggcgggcat ctttaccgca
    16081 ggcacggcgc agcggctcgt caacatggaa ggctatctcc ccgggcggcg ggtcgtgatc
    16141 ctgggcagcg gcgatatcgg gcttatcatg gcgcggcgca tgacgctgga gggcgcaaaa
    16201 gtcctctgcg tggccgaact gatgccctac tccggcgggt tgaagcgcaa tattgtacag
    16261 tgcctcgatg atttcgacat cccgttgaaa ctgagtcata ccgtggtgga tatccagggc
    16321 aaagagcgcg tgaccggcat cacgatcgcg caggtcgaaa acggcaagcc catccccggc
    16381 accgaggaac attacgactg tgacacgctg ctgctctcct gcgggctgct gcccgagaat
    16441 gagctgagcc gcgcggcggg ggtggcgctc agtcccatca ccggcggccc cagcgtgaac
    16501 gagagcctgg agacgaacat ccccggcgtg tttgcggcgg gcaacgtgct ccacgtacat
    16561 gacctggtgg actatgtctc cgaggaagcg ggggcagcgg gcaaacacgc cgccgcgtac
    16621 attctgaaaa gcgaacacgc agccgggcgt acactgcctg tgcactgtga aaacggtgtg
    16681 cgctacaccg tgcccacggc catccgcccc gactgcgcgg gtgacaccgt gacgctgcgg
    16741 ttccgggtgg gcggcgtgta caaaaacaaa aaaatcgcgg tctacctggg cgatacctgc
    16801 attttcagcc gccggcggcc tgtgctggcc cccggtgaga tggagactgt gcggctgaaa
    16861 tccgacctgc tgcgcggcgc aggcgacgcc gtgaccgtga ctttggagga gggctgacga
    16921 tggaaaaacg tgaattgacc tgcatcggct gcccgatggg ctgcgccctg acggtggagc
    16981 tggacggcgg cgcggtggtg tcggtgcgcg gcaacacctg cccgcggggc gacgcctacg
    17041 cccgcaagga agtgacggcc cccacccgca tcgtgaccac gacggtgcgc gtgacagacg
    17101 gcgcgttggc cgccgtgagc tgcaagaccc gcggcgatat cccgaagggc aagattttcg
    17161 atatcatccg tgcgttgaaa gcggtggagg tcgctgcgcc ggtggtgatc gggcaggtga
    17221 ttttgcccaa tgccgccgac accggcgtgg atgtgatcgc aaccaagaat gtggaaaaac
    17281 gaggataaca cagaaacccc gcccgggcgt gatagcccgg gcggggtggt ttttatggga
    17341 aaaatttttg gttgtgatgg tgggcaccgg ggcagagcgt gtaggggcga acattgttcg
    17401 cccgcagaac cacgccgcgg caaccctttt accgggaaac accggcacat ccgcccctca
    17461 tccggccctg cggggccacc ttccccctcg ggaggaaggc tggaaaattt taccccagca
    17521 aattcgtcat gctctttaag ctgacgccga gggtcgaggc gttgtgcagc agggcggagg
    17581 tggcgggagg caggatgccc atggcaccca gcacgatcag ggagctgttg aagctcatga
    17641 taaagcggta gttcgccttc gtgcgctttt gcaggccgtt ggcgatgctc ttcaaaatca
    17701 ccagctcgcg caggctgtcg gcggcaatcg tgatatccgc aatttcccgg gcaatggccg
    17761 cgccgtcgct gatggcaatg ccgacgtcag cggcggacag ggcaggggag tcgttgatgc
    17821 cgtcgcccag catgataacg gtgtgcccgg cggctttttc ggcctccaca aagttggctt
    17881 tgtcctcggg caagacctcg gagtagtagc ggtccacgcc gacctggttg gcaatcaccg
    17941 acgccgtgcg gtcgttgtcg ccggtcatca tgacggcctt gcggatgcct aagctgcgca
    18001 gttggcgcag cacctcggcg gcttcctcgc gcagggggtc ggcaatgcag atgacaccgg
    18061 ccagcacacc gccgatggca agatacaggt gggaatattc cggcggtaat gcgtcaaatt
    18121 tatcgcactc ggccgtcggg atgcagcagc cctcgtcctc aaagacaaag tgctggctgc
    18181 cgatgacggc cttctcgccg ccaatctggc tggcgatgcc gtgggcgacg acgtactgca
    18241 cctcgctgtg catctcgtcg tggcggatgc ccttcgcggc ggccgcctgg acgacggcgt
    18301 tcgccatcga gtgggggtag tgctcttcca ggcaggccgc gatctgcagg acttcgtcct
    18361 cagtgcgggt gccgaagggc acgacctgca cgacggtggg ggtcgcgtgg gtcagcgtgc
    18421 cggtcttgtc aaacacgatg gtgtcggcg
//
LOCUS       DBMDSQ010000007        18415 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_993_length_18415_cov_2.364038, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000007 DBMDSQ010000000
VERSION     DBMDSQ010000007.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 18415)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 18415)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18415
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_993_length_18415_cov_2.364038"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..223)
                     /locus_tag="ACZPAX_00555"
     CDS             complement(<1..223)
                     /locus_tag="ACZPAX_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005943364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding protein"
                     /protein_id="HIR1112419.1"
                     /translation="MELLKLKILDAEGQTLMTAPGAEQVSLVYMNAYKPGDRVALEIG
                     TPGQYIVIQFEDTMAPALVYVVKREINFHI"
     gene            complement(237..2057)
                     /locus_tag="ACZPAX_00560"
     CDS             complement(237..2057)
                     /locus_tag="ACZPAX_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005920150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III domain-containing protein"
                     /protein_id="HIR1112420.1"
                     /translation="MLMQRMTPRFDAMRPTLYPPYRDRAAWQALPGAARWQAAGQAAL
                     RDAAAVPQLPLSLWLQFTQNGDRARWEHAYFARRRTLCALAMAEAVTGDGSYLPAIAD
                     AAWAICEESAWQLPAHNSYIRDTPQLPLPDVTRPIVDLFAAETGALLATVYGLLGAAL
                     DEYAPGLAARLQHEVERRILTPYRTEHFWWMGRDDEPMCNWTPWCTQNVLLAAAQCAP
                     VEALPAYVGQAAYSLDCFLKDYGPDGCCSEGAQYYRHAALTMFNALDLLCKIAPGVFD
                     DIWAEPKIKNMAEYIVNMHIAGPYYLNFADCSPLAGARGVREYLFGKRVGSLPLMTLA
                     AHDWTETLRTPDPDRLHHPDDSEGINLFYHLQTALAEQEVTAFALRASPAAPGDVWYP
                     SVGILVCRRGAYALGAKAGSNADSHNHNDVGSVTLYKNGAPLLIDVGVETYSKKTFSP
                     QRYEIWTMQSGWHNLPEFDPDGAAYQQQPGAAFAAAGVAVADTLDSIAMDLAPAYGAV
                     PGLGYYRRAVRLTADGLTLCDETDYPGEVALTLMSVEKPAADGSTVQFGTLAAARADG
                     AVRIVTEAVAVTDARLRQAWPDTLYRTRLYFTGQLALTVF"
     gene            complement(2026..3309)
                     /locus_tag="ACZPAX_00565"
     CDS             complement(2026..3309)
                     /locus_tag="ACZPAX_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015565852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR1112421.1"
                     /translation="MTKIFLVDDEPLVLIGMQGMLNWAELGYTVVGTARNGAEALKAI
                     GETYPDIVVSDIRMPVMDGFTLAESCHKDGSALPVFIMLTSYEEFDYVKRSMRLGVVD
                     YLVKIDLTAESLTAALDKARAAVEKERRLQAPAAPAVSMDSYRDRLFLQLYGGLLTDR
                     AAFDRQCAELGLDFHAPQYVVALGSIRTPELSTEQLLTLSTGVTRMAAETLPKYRPCR
                     VIGMDLQHFSALLPLQEGEDPETALTPALQATGQILYNYFSSQIYWAVGRAVPDILQI
                     SQSRQDALAAQQLLRSESPLAFYHEKPNTPLDHRAQVVAQVQQYIRAHLAEKLTLNDV
                     AAVFNFSPNYLSQLFAQNSERGFVEYVTTARIAAAKELMAATDLKIYEVSSRVGFDSA
                     FYFSKVFKKLEGASPREYIQRMKGSYVDAKDDAAL"
     gene            complement(3401..5236)
                     /locus_tag="ACZPAX_00570"
     CDS             complement(3401..5236)
                     /locus_tag="ACZPAX_00570"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR1112422.1"
                     /translation="MGKFNLHRGSLQLRVLTLTLLFTLGVSIVITVSNMHQLNAEWER
                     TTLLNAEYALQTAAAAIYRDLDEVDDLASWCAYNPSMRTYLLTDSAGNNQALSMYPMI
                     SAKYNSMRTLPYIQRFMLINAAGRPMMFGTAATNVVALTPELLQTIPGYNEASYGWAY
                     ITRDPLALTTQAAQTIPICRALTLPGTDRTAHVCIFVSPSVITAPLRGFTLSEGGKLL
                     WEMGEHIYTVQNNTLTELDTDRTYYKDTRPFDAETLDSLTEVYERGSGVNTQLIVRYP
                     IGVHDLYLIEVLPNGPLQRQIPYLSASLIFSLAAILALGSALAFLLHRMIARPITALQ
                     SRIEAVGSGDFSADPAIEWDNELGDIGRGINKMSGNITALMDRRLEDEKQKQDLEYRM
                     LQNQINPHFIYNTLNSIKWMATIQHAPGIAEMVTALSRLLKSVSKGNERLVPLYEEFA
                     LLNDYFTIQQYRYGGTITLDVSYIEDENLTHSCLIPRFTLQPLVENAIFHGIEPKGSA
                     GEVTLRVERDAANGDVLILLSDDGVGMTPAQAAKALQEPGPEEAAAKYRHVGIWNVHR
                     RLQYSFGDAYGLRIESEPGVGTTVTVRLPDAKKASPAGEAVRKAD"
     assembly_gap    5476..5485
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5602..6939
                     /locus_tag="ACZPAX_00575"
     CDS             5602..6939
                     /locus_tag="ACZPAX_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015538150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR1112423.1"
                     /translation="MRKMQRFAALGLAGTMALSLAACGGSSASTTDSTPASTASSTEA
                     TAESTASGDSVTLNWALWDKDSTAYWAALADGYMASHPGVTIEMTDLGSTDYMTQLAT
                     QLAGGNGELDVLSIKDIPGYSNLINLGMLEPLSGKLTTDESKFNGVLDQLTAEDGNYY
                     AVPFRSDFWVVYYNKDIFDQAGIEYPTNDMTMADFDAKIREVYEKTGIYGNIYHTWRS
                     TTTLFGILDGKNTVIDGNYDFLKPYYEQVMAEQTDGVIPNYGEQKTANLHYSGAFENG
                     QAAMCNMGSWFIATLQKYNAEASANGVQPVNFGIVKYPHPDGAPAGSTLGTVTSLAVN
                     ANSEKKEAALDFVNWCASEEGAKAVAAVGTFPAVASDETNKIISSTEGFPSDENSLEA
                     LNTTAIYLEMPLSDKASEIETILNTEHDAIMTESETIDEGIANMNEQVQALLG"
     gene            7078..7995
                     /locus_tag="ACZPAX_00580"
     CDS             7078..7995
                     /locus_tag="ACZPAX_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015357942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR1112424.1"
                     /translation="MAETTQAVQVNPKRGMKKSTRDSLIAYSFIAPNFIGFCVFTLVP
                     MIFAIALSFCNWDGVHPVEFTGISNFVALLSDKTFKAAFVNTLVYAVGTVPLTLVCSL
                     GLAMLLNQKVKCRNFFRTVSFFPYVASLVAVAAVWNMIFSPSMGPVNQILAGLGVENL
                     PKWAAGKDTAMLTVILFSVWKNMGYYMVIYLAGLQGTNPELEEAAELDGANKWQIFWN
                     VTLPQLKPTTFFVIIMLTIASFKVYDQMYMITQGGPGNATMTLVYYIYNVAFVNTPKY
                     GYASAVSMVLFVLVLFVTIIQFKGSSNND"
     gene            8013..8876
                     /locus_tag="ACZPAX_00585"
     CDS             8013..8876
                     /locus_tag="ACZPAX_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015357943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR1112425.1"
                     /translation="MATTAVKEKTGSMKAQHIVSRVLIYALLIIVAALMLIPFLWMLS
                     ASFKLNKDVFTFPIEWIPSNPRPQNYVDIWTKIPLLTFIKNTAFLTIVVTILQVLTSS
                     FAAYAFAKLNFKGKNVLFLGYIATIAVPWQAYMVPQFMMMRSWHLNNTHLAIICLQAF
                     SAFGVFMMKQFYEGVPTELCEAARIDGLSEYGIYARIMLPLSLPAVSTLVIFTFVNTW
                     NDFLGPLIYLTKTELKTIQIGLRMFISQYSAEYGLIMAASVIALIPVLIVFLSLQKYF
                     VQGVASSGLKG"
     gene            9089..10252
                     /locus_tag="ACZPAX_00590"
     CDS             9089..10252
                     /locus_tag="ACZPAX_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015538153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HIR1112426.1"
                     /translation="MKTFPNKILTKSQAEQALDTAVALVRQNLPLYTDHCQNHSSVHD
                     IYPQCENNQWTCGFWPGEVWLSYERTGDAAFKNTALTLVDSFLYRIENKIEVDHHDMG
                     FLYSPSCVAAYKLTGSEKGRRAAILAADQLCTRFQEKGSFFQAWGPLGAKDNYRYIID
                     CLLNVPLLYWASETTGDAHYAELAHRHVTTCLANSIRPDGSTYHTFFMDPVTGAPVRG
                     ATCQGYKDDSCWARGQAWGVYGTAISYRYTKRPEYLEAFRRVLSYYLDRLPEDLIPYW
                     DMTFTEGDTEPRDSSSASIVACGLLEAAKYANPDQAAEYTLLAAQMLGSVAEHYAVRQ
                     GPQGIGLVRHGTYSKKSPYNTCTPEGVDECVSWGDYFYMEGLTRLTKDWTLYW"
     gene            10294..12006
                     /locus_tag="ACZPAX_00595"
     CDS             10294..12006
                     /locus_tag="ACZPAX_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015565858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HIR1112427.1"
                     /translation="MNLQTKQDFTALAMRFLSPLLPLYSPGGALLHLGDTGATYPQRT
                     IGMEAFSRPLWALAPLWAGGGRADAFLPIYQRGLVNGTDPDSPEYWGDPNDYDQLFVE
                     MAALACAILETPESVWNPLTDTEKAKLGKWLDTINHHDLPGCNWLLFRVLVNLALDSV
                     GMPCSMQQAAADMAEVDKWYVGDGWYSDGPAEVKPQKDYYNPWAIQYYTVLYSVFAAR
                     RDPARAALYRERAVQFGRQFAQWFDPDGAALPFGRSLGYRIGQSAFYAACIWAGLEPL
                     PLPVMKGIIVRNLAWWLARPIFDRDGVLTIGYGYPQMYMAEQYNAPGSPYWGLKVFLL
                     LALPDDHPFWACEAAPLPEELTRPGVIPQPSADLLLQRLPDGQLNAYSPANYEKGDHG
                     QFTEKYGKFVYSTRFGFSASRSYVQLEQTAPDSMLAFVIDGWTFVRRHSDKFVLLGDR
                     LLSEWRPFPGIKVTTELVPTAWGHTRTHTVESNIACTAYDCGFAVPKFAAGFAQSAAG
                     SEAEAKNAACRCVVKGAAGQGVVINAAPNTNLYDPNTVIPAVRYEIPIGTAVLNTRVE
                     SRHN"
     gene            12100..13848
                     /gene="uidA"
                     /locus_tag="ACZPAX_00600"
     CDS             12100..13848
                     /gene="uidA"
                     /locus_tag="ACZPAX_00600"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015538152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HIR1112428.1"
                     /translation="MYPQTNAVRSVRDLSGVWDFRFNANEPWQPIAVPASYNDQSPDP
                     EFRRHYGMAYYRTTFTVPEGARRVLRFDAVTHNAVVRLNGQEIVTHRGGFLPFEADIT
                     ALAKPGDTVTLEVEVDNRIGHGTLPVGNEGGTAFFGSDNAGIPAVEAGKARQQMQGVN
                     LPNFDFFNYAGITRPVRLYTTPAAYIRDITLVAGADGKLHYTVDTVGEGNVQLTVLDA
                     NGRAVADADGAAGTLQVKNARLWQPRPGTPYLYTALVRFGTDEYRQPFGFRSVEVRGH
                     QFLINGEPFYFKGPCKHEDSLFRGRGYDACVTVTDLKLYEWLNANCLRMSHYPYAEEV
                     YDLCDRLGIVVIDETPAVGIGAGAACDPYKTFPLKNYHSAVLKAMIDRDKNHPCVVLW
                     SLGNEPDTEHFPESARDYWRPLYEQAHAQDPQGRPVTMVCCQNDYTKDITTRTMDVVC
                     INRYYGWYNLSGDLDNASYAFRQELDFWKGIDKPLILSEYGADTVAGLHGVAPEMFTE
                     EFQVEYYKAINACLDSYAFVVGEWPWNFADFSTQQGPMRVGSCNRKGLFTRERTPKLA
                     AHYFRDRWAKKQPNDR"
     gene            13863..16625
                     /locus_tag="ACZPAX_00605"
     CDS             13863..16625
                     /locus_tag="ACZPAX_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HIR1112429.1"
                     /translation="MTPELKWLTDPTVFAVNRLDAHSDHVCYRSEAEIRLGGSSLRQS
                     LDGSWRFAYSACPAARPADFYREDADFSAFGTICVPGHIELQGYGQIQYTNTLYPWDG
                     RSYLRPPQIDWDNTPVGSYLREFDLDEGLRGQRVCVSFQGAEQALYVWCNGHFVGYAE
                     DSFTPSEFDLTPYIRQTGNRLCVEVYKHSSASWLEDQDFFRFSGLFRSVYLYAKPKVH
                     IRDIWLKAELAEGNTTGLLTPIVKLDGETDGARVHLRLTDGEEFALFDEDIDGSTIEL
                     SAIHPWSHETPVLYHALLTLYDSDGALQEVLPYDIGFRRFEMKDGIMCLNGSRIIFNG
                     VNRHEWNPEKGRAIDESDMHAAMAVFKRNNINAVRTCHYPNQSRWYDLCDKNGIYMID
                     ETNLESHGSWQKLGVVEPSWNVPGSLPEWRDCVVDRARSMFERDKNHTAVLIWSCGNE
                     SYAGEDIRAMAAFFRENDPGRLVHYEGVFQCRAFDDISDMESRMYASPASIREYLDAD
                     PKKPFILCEYMHDMGNSLGGMESYIALIDRYEKYQGGFIWDYMDQALWHTDAMGRRVL
                     GYGGDFNERTTDYNFSGNGIVYADGTEKPAMQEVRYWYDTPERRAVHDAHNKLAAERS
                     AAALAAAWQKRPTRPLTVTEGDGNIGVKGSGFEVLFSISEQGPVSLRKNGAEWLWRAP
                     RPAFWRASTDNDRGCSFPQRAAVWMGADVFVQRMGFTVQEKSAQCVRVQYRFGVPGVP
                     GAQVEMIYTVEAQGALRLDAVYHGVPGAPELPCFGIKFETAAPVVRTRWTGLSGETYP
                     DRCKGGVFGCHEEPPHIEPALVPQDCGLHMDTQQVNLQLADGKTLTLESTGEAFAFSA
                     LPNTAQQIEAAQHPCELPETGRTCVTVLGAARGVGGIDSWGTDVEAPYHVNGEQQHSV
                     SLRILL"
     gene            complement(16764..18200)
                     /locus_tag="ACZPAX_00610"
     CDS             complement(16764..18200)
                     /locus_tag="ACZPAX_00610"
                     /inference="COORDINATES: protein motif:HMM:NF012798.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIR1112430.1"
                     /translation="MKDTSKTSLRRGHNEGNIRQRTDGRWEVRLSAGIDYKTGKPRRT
                     STCCNTRQEAIAILQQQAHEVRTQGWRDPMSVTLGEWYEYWLETYMKDTVKQSTYASY
                     RSYLNKHFCVLGKILLKKLEPHTLQEFYNYKFREEGLSPKTLRNYHMALHKCLQQAVK
                     EHLLVYNPCDAVTLPSGEKPEVVVFTNDQQRALVQASYRHRYGVFIRLDLCTGLRMGE
                     LLALKWEDIDFSTAQLHVRRTINRLAKYEAHDGENKTEIVFGTPKTKNSRRTIPLTRT
                     MADELARWKQQQAQDKIRAGDKYTDYGFIVTNEFGHYFEQKTFKDYYDRLLKDADIGH
                     FTFHALRHTFATRALERGMDYKTLSAILGHYSVAFTMDTYVHSMDEHKRHEMDKMDDM
                     FGMQYSISVENQPYPVLCTLSPDGCTAHVPDFPKITAQAPTLDAALLEVKQQIQKALH
                     QYKNPPIPTKQDQIVVPTNSVLVLVKAS"
BASE COUNT         3581 a         5589 c         5560 g         3675 t
ORIGIN      
        1 gaatgtggaa gttgatctcc cgcttgacga catagacgag cgccggggcc atcgtgtcct
       61 caaactggat cacgatatac tgcccgggtg tgccgatctc cagtgccacg cggtcccccg
      121 gcttgtaggc gttcatgtac acaaggctga cctgctccgc accgggcgcg gtcatgaggg
      181 tctggccctc ggcgtcaagg atctttaact tcaaaagctc catgggtatc atcctttcag
      241 aaaacggtaa gggcaagctg cccggtgaaa tacaggcggg tgcggtagag ggtgtccggc
      301 caggcctgac gcagccgggc atcggtgacg gctaccgcct cggtcacgat gcgcacagcc
      361 ccatccgcac gggcggccgc taaggtgccg aactgcacgg tgctgccgtc tgcggcgggc
      421 ttttcaacgc tcatcagcgt caaggcgacc tcgccggggt agtcggtctc atcgcacagg
      481 gtcagcccat cggcagtcag gcgcacggcg cggcggtagt accccaaccc cggcacggcg
      541 ccgtaggcag gggcaaggtc cattgcgatg ctgtccaatg tgtcggccac ggcgaccccg
      601 gcagcggcaa aggccgcacc gggctgctgc tgatacgccg caccgtcggg gtcaaattcc
      661 ggcaggttgt gccagccgga ctgcatcgtc cagatctcat accgctgcgg gctgaaggtc
      721 tttttgctgt aggtctccac acccacatcg atcaggagcg gtgccccgtt tttatacagc
      781 gtcacgctgc ccacatcgtt gtggttgtgg ctgtcggcgt tgctgcccgc cttggcgccc
      841 agtgcataag cgccgcggcg gcagacgaga ataccgacgc tcggatacca gacatccccc
      901 ggcgcggcag gggatgcgcg cagggcaaag gcagtcacct cctgctcggc cagtgcggtc
      961 tgaagatggt aaaacaggtt gatgccctcg ctgtcatcgg ggtggtgcag tcggtcgggg
     1021 tcaggggtgc gcagcgtttc agtccagtcg tgggcggcca gcgtcatcag cggcaggctg
     1081 ccgacccgct tgccgaacag gtactcccgc acgccgcggg ccccggccag cgggctgcag
     1141 tcagcaaaat tcaggtagta cggcccggca atgtgcatgt tgacaatgta ctcggccatg
     1201 tttttgatct tcggctcagc ccagatgtcg tcaaagacgc cgggggcgat tttgcacaag
     1261 aggtccagcg cgttgaacat cgtcagcgcg gcgtggcggt agtactgtgc gccctcgctg
     1321 cagcagccgt ccgggccgta atccttcaaa aagcagtcaa ggctgtaggc ggcctgcccg
     1381 acataggcgg gcagcgcctc aaccggggcg cactgcgcgg cggccagcag gacattctgg
     1441 gtacaccacg gtgtccagtt gcacatcggc tcgtcatcgc ggcccatcca ccagaaatgc
     1501 tcggtacggt agggcgtcag gatgcggcgc tcgacctcat gctgcagccg tgccgccagc
     1561 ccgggcgcgt attcgtcaag ggcggcaccc agcaggccgt acacagtggc cagcagtgcg
     1621 ccggtctcgg cggcaaacag gtccacgatc ggccgcgtta catcgggcag cggcagctgg
     1681 ggcgtgtcgc ggatatagct gttgtgggcg ggcagctgcc acgcgctttc ctcgcagatc
     1741 gcccatgcgg catcggcgat agcgggcaga tagctgccgt ccccggtcac ggcctcggcc
     1801 attgccagcg cacagagggt gcggcggcgg gcaaaatagg cgtgttccca tcgggcgcgg
     1861 tctccgtttt gggtaaattg cagccacagc gagagcggca gctgcggcac tgcggcggca
     1921 tcgcgcaggg cggcctgtcc cgccgcctgc cagcgcgcag cgcccggcag ggcctgccac
     1981 gccgcgcggt cgcggtaagg cgggtaaagc gtggggcgca tcgcgtcaaa gcgcggcgtc
     2041 atcctttgca tcaacatacg atcccttcat tctctggata tattcgcggg ggctggctcc
     2101 ctccagcttt ttgaacacct tgctgaaata gaaggcgctg tcaaagccta cccggctgct
     2161 gacctcatag atcttcaggt cggtggcggc catcagctcc ttggcggcgg cgatgcgggc
     2221 ggtggtgaca tactccacaa agccgcgctc actgttttgt gcaaaaagct ggctcaggta
     2281 gttggggctg aagttgaaca ccgcagctac atcgttgagg gtcagctttt cggccagatg
     2341 tgcccggatg tactgctgca cctgcgccac gacctgtgcg cggtgatcca gcggggtgtt
     2401 gggcttctca tggtaaaagg caaggggaga ctcgctgcgc agcagctgct gggcggccag
     2461 tgcatcctgc cggctttggg agatctgcag aatgtccggc accgcgcggc ccacggccca
     2521 gtagatctgg gagctgaaat agttgtaaag gatctgcccg gtggcctgca gggcgggggt
     2581 cagcgcggtc tcggggtcct cgccctcctg caggggcagc agcgccgaaa aatgctgcag
     2641 atccatgccg atcacgcggc agggacggta tttgggcagc gtttcagccg ccatgcgggt
     2701 gacgccggtg ctcagggtga gcagctgctc ggtcgaaagc tccggggtgc ggatgctgcc
     2761 cagcgccacg acatactgcg gggcgtggaa gtccagcccc agctcggcac actggcggtc
     2821 aaaggcggcg cggtcggtca gcagaccgcc gtacagctgc aaaaacagcc ggtcgcggta
     2881 gctgtccatg ctcacggcgg gggcagcggg ggcctgcagc cgccgctcct tctccacagc
     2941 ggcgcgggcc ttgtccagcg cggcggtcag gctctcggca gtcaggtcga tcttgacaag
     3001 gtagtccacc acgcccagcc gcatgctgcg ctttacatag tcaaattcct catagctggt
     3061 cagcatgatg aataccggca gggcagagcc gtccttgtgg cagctctccg ccagcgtaaa
     3121 accatccatg accggcatac ggatgtcgga aacaacgatg tcggggtagg tctctccgat
     3181 ggccttgagg gcctcggccc cgttgcgggc ggtgccgacc acggtgtagc caagctcggc
     3241 ccaattcagc atgccctgca taccgatgag gaccagcggc tcatcatcga ccaagaaaat
     3301 ttttgtcacg gtaacacctc atttttgggg atgccttcct gttggggaga ggcaggggta
     3361 taaacccatg ggcgggcaga cgcggcaaag ccgggctgca tcagtctgct ttgcggacgg
     3421 cttcccctgc gggggaggcc tttttggcgt ccggcagccg caccgttacg gtcgtgccta
     3481 cgcccggttc actctcgatg cgcaggccgt aggcatcgcc gaagctgtat tgcagccggc
     3541 ggtgaacatt ccagatgccc acatggcggt atttggcggc ggcctcctcg gggccgggct
     3601 cctgcagggc tttggcggcc tgcgcagggg tcatgcccac accgtcatcg ctcaggagga
     3661 tcagcacatc gccgtttgca gcgtcccgct cgacccgcag ggtcacctca ccggcgctgc
     3721 ccttcggctc aatgccgtgg aagatggcgt tctcgaccag cggctgcagc gtaaagcggg
     3781 ggatcaggca gctgtgggta aggttttcgt cctcgatata gctgacatca agggtgatcg
     3841 tgccgccgta gcggtactgc tggatcgtga aatagtcgtt gagcagggca aactcctcat
     3901 acagcggcac aagccgctca ttgcccttgg aaacgctttt cagcaggcgg gagagcgccg
     3961 tgaccatctc ggcaatgccg ggcgcatgct ggatggtagc catccacttg atgctgttga
     4021 gggtattgta gatgaaatgc gggttgatct ggttttgcag catccggtat tcaaggtcct
     4081 gcttttgctt ttcgtcctca aggcggcggt ccatcagggc ggtgatgttg cccgacattt
     4141 tattgatgcc gcggccgatg tcgcccagct cgttgtccca ctcaatggcc gggtcagcgg
     4201 aaaaatcgcc gctgccgacc gcctcgatcc ggctttgcag cgccgtgatc gggcgggcga
     4261 tcatcctgtg cagaagaaat gccagcgcac tgcccagcgc cagaatggcc gccagactga
     4321 atatgagtga ggccgacaga tagggaatct gccgctgcag cggtccgttg ggcaggacct
     4381 cgatcaggta gagatcatgc accccgatgg gataccgcac gataagctgg gtgttgacgc
     4441 cgctgccgcg ctcgtatacc tcggtcaggc tgtcgagggt ctcggcgtca aaggggcggg
     4501 tgtccttata ataagtacgg tcggtgtcaa gctcggtcag ggtgttgttc tgcacggtgt
     4561 agatatgctc gcccatctcc cacagcagct tcccgccctc tgacagggta aagccgcgca
     4621 gcggcgccgt gatgacggaa ggggaaacaa agatgcagac atgcgctgtg cggtcggtgc
     4681 cgggcagggt cagggcgcgg cagatcggga tcgtctgcgc cgcctgagtg gtcagcgcca
     4741 acgggtcgcg ggtgatatac gcccagccgt agctggcctc gttgtagccg gggatcgtct
     4801 gcagcagctc cggggtcagc gccacgacat tggtggcagc cgtgccgaac atcatcggcc
     4861 gcccggcggc gttgatgagc atgaaccgct ggatatacgg cagcgtgcgc atggagttgt
     4921 atttggctga gatcatcggg tacatcgaaa gcgcctgatt gttgccggcg gaatcggtca
     4981 gcaggtaggt gcgcattgac ggattgtagg cgcaccagct tgccagatcg tccacctcgt
     5041 caaggtcgcg gtagatggcg gcggctgcag tctgcagggc atactcggca ttcagcaggg
     5101 tggtacgctc ccactcagcg ttgagctggt gcatgttgct gactgtgatg acgatcgaca
     5161 cgcccagcgt gaacagcagg gtcagcgtca gcacccgcag ctgcaggctg ccccgatgca
     5221 ggttgaattt gcccataaag gctccttgta tagtataata aggtgagaag cagctttttg
     5281 aatggtcctg tatatacata tataccctat cagacaggct gctgtcaaga ataaaactgt
     5341 acaaatatga caaaatagcg cgtatttgct tgtctataac tgtaagaaag atgaatattg
     5401 caggtttctt gaaatattac atgttttggc ggtctccttt gcggaactgc cgtaaatatt
     5461 gcaaaagtaa cccaannnnn nnnnnaatat accatcgctt tcggcgggca gcgggtgtat
     5521 gataatgaca acgaaaggga caggccctct gcagaaagcc ttcccgcaag taacgatttc
     5581 tttttaggag gagaaattag tatgcgtaaa atgcaaagat tcgcagccct tgggctggcc
     5641 ggcaccatgg ccctgagcct ggcagcctgc ggcggcagct ccgcttccac cacggactcc
     5701 acccctgcaa gcacggcatc ttccaccgag gccaccgcag agagcacggc ctccggcgac
     5761 agcgtcaccc tgaactgggc gctgtgggac aaggactcca ccgcctactg ggcagcgctg
     5821 gcagacggct acatggccag ccaccccggc gtcaccatcg agatgaccga ccttggctcc
     5881 accgattaca tgacccagct ggccacccag ctggccggcg gcaacggcga gctggatgtc
     5941 ctctccatca aggacatccc cggctactcc aacctgatca acctcggtat gctggagccg
     6001 ctgagcggca agctgaccac cgatgagagc aagttcaacg gcgttctcga tcagctgacc
     6061 gccgaggacg gcaactacta cgctgttccg ttccgctctg acttctgggt cgtctactac
     6121 aacaaggaca tcttcgatca ggccggcatc gagtacccca ccaacgatat gacgatggcc
     6181 gactttgacg ccaagatccg cgaggtctac gaaaagaccg gcatctacgg caacatctac
     6241 cacacctggc gctccaccac gacgctgttc ggcattctgg acggcaagaa caccgtcatt
     6301 gacggcaact acgacttcct gaagccctac tacgagcagg tcatggccga gcagactgac
     6361 ggcgtcatcc ccaactacgg cgagcagaag accgccaacc tgcactactc cggcgcgttt
     6421 gagaacggtc aggccgctat gtgcaacatg ggctcctggt tcatcgctac cctgcagaag
     6481 tacaacgccg aggcttccgc caacggtgtc cagcccgtca acttcggcat cgtcaagtat
     6541 cctcatccgg acggcgcacc tgccggctcc acgctgggca ctgtgaccag cctggccgtc
     6601 aacgccaact ccgagaagaa agaggctgcg cttgacttcg tgaactggtg cgcttctgag
     6661 gaaggcgcca aggccgttgc cgcagtcggc accttccccg ccgttgccag cgacgagacc
     6721 aacaagatca tctcctccac cgagggcttc ccctctgacg agaactccct cgaggcactg
     6781 aacaccaccg ccatctacct tgagatgccg ctgtccgaca aggcctccga gatcgagacc
     6841 atcctgaaca ccgagcatga cgccatcatg accgagagcg agaccatcga cgagggcatc
     6901 gccaacatga acgagcaggt tcaggcactg ctgggctgat ttgagtgatc acggcccctg
     6961 caagaaacat ttgcaatctt ccaacttttt cataagtttt ccttcttctt cgtaaagggg
     7021 cgggcgggcg cgcctgcccc ttctcttatg cagctaaccc ccgaggaggt atgatccatg
     7081 gccgaaacca cacaggctgt acaggtgaac ccaaagcgcg gcatgaaaaa atccacgcgg
     7141 gacagcctga tcgcctacag cttcatcgca ccgaacttca tcggcttctg cgtctttacg
     7201 ctggtgccca tgatcttcgc catcgcgctt tccttctgca actgggatgg tgttcatccg
     7261 gtagagttca ccggcatcag caactttgtc gcgctgctca gtgataagac cttcaaggcg
     7321 gcgttcgtca atacgctggt ctacgccgtc ggcaccgtgc cgctgacgct ggtctgcagc
     7381 cttggtctgg ccatgctgct gaaccagaag gtcaagtgcc gcaacttctt ccgcacggtc
     7441 tcgttcttcc cgtatgttgc ctcgctggtc gcggtcgcgg ccgtctggaa catgatcttc
     7501 agcccctcga tgggcccggt caaccagatc cttgcgggcc tcggcgtgga gaacctgccc
     7561 aagtgggccg ccggcaagga caccgctatg ctgacggtca tcctcttcag cgtatggaag
     7621 aacatgggct actacatggt catctacctt gccggtctgc agggcacgaa ccccgagctg
     7681 gaggaggctg ctgagctgga cggcgccaac aagtggcaga tcttctggaa tgtcacgctg
     7741 ccgcagctca agcccacgac cttctttgtc atcatcatgc tgaccatcgc ctccttcaag
     7801 gtgtatgacc agatgtatat gatcactcag ggcggcccgg gcaatgccac gatgaccctt
     7861 gtctactaca tttataatgt tgcctttgtc aacacaccca agtacggcta tgcaagcgcc
     7921 gtctcgatgg tgctgtttgt actggtgctg tttgtcacca tcattcagtt caagggctcg
     7981 tccaacaacg actaaggaaa ggaggataca ccatggctac taccgctgta aaagaaaaga
     8041 ccggctcgat gaaggcgcag catatcgtca gccgcgtgct gatctacgcg ctgcttatca
     8101 ttgtggcggc actgatgctc atcccgttcc tgtggatgct gtctgcatcc tttaagctga
     8161 ataaggatgt tttcaccttc cccattgagt ggattccctc caacccgcgc ccgcagaact
     8221 atgtggatat ttggaccaag atccccctgc tgacctttat caaaaacacc gccttcctga
     8281 ccattgtggt cacgattttg caggtgctga cctcctcgtt tgccgcctat gcgttcgcca
     8341 agctgaactt caagggcaag aatgtgctgt tcctcggcta tatcgcaacc atcgcggtgc
     8401 cctggcaggc ctacatggtg ccccagttca tgatgatgcg ttcctggcat ctgaacaaca
     8461 cccaccttgc catcatctgt ttgcaggcgt tcagcgcgtt cggcgtcttt atgatgaagc
     8521 agttctatga gggcgtcccc accgagctgt gcgaggccgc gcgtatcgac ggcctgtcgg
     8581 agtacggcat ctacgcccgc atcatgctgc cgctgtcgct gccggctgtg tccacgctgg
     8641 tcatcttcac ctttgtgaac acatggaacg acttcctcgg ccccctgatc tacctgacca
     8701 agacggagct gaaaaccatc caaatcggcc tgcgcatgtt catcagccag tattccgcag
     8761 agtacggcct gatcatggcg gcctcggtca ttgcgctgat cccggtgctg atcgtgttcc
     8821 tctccctgca gaaatatttc gtgcagggcg ttgcatccag cggcctgaag ggctgataca
     8881 ctacccattt gtacggggcg caaggcggaa agccctgcgc cccttttttt aagataaaag
     8941 ataaaaatta tggtggcgcg ttccgctgcg ctccacgcaa aaaaacaaaa ccgcgagccc
     9001 cgcattttgt tttcgcccaa ggcgaaaact ccaccttatt tattatctat tatcttttat
     9061 ctattctcta agaaaaaaga ggtttcccat gaaaacattc cccaataaaa tcctgacgaa
     9121 gtctcaggcc gaacaggcgc tggataccgc cgtggctctt gtgcggcaga atctgccgct
     9181 gtatacagac cattgccaga accattccag tgttcatgac atctatcccc aatgcgagaa
     9241 caaccagtgg acctgcggct tctggccggg cgaggtctgg ctcagctatg agcgcaccgg
     9301 tgatgcggcg ttcaaaaata ccgcgctgac gctggtggac agctttctgt accggattga
     9361 aaacaagatc gaggtagacc accacgatat gggctttctg tacagcccgt cctgcgtggc
     9421 agcctacaag ctgaccggca gcgaaaaggg ccgccgcgcc gccattctgg ccgctgacca
     9481 gctctgcacc cgctttcagg agaagggcag cttctttcag gcatggggtc cgctgggcgc
     9541 taaagacaac taccgctata tcattgactg cctgctcaat gtgccgctgc tctactgggc
     9601 cagcgagacc acgggcgatg cccattatgc cgagctggcg caccgccatg tcacgacctg
     9661 tctggccaac tccatccggc cggacggctc gacctaccac accttcttta tggaccccgt
     9721 gaccggcgcg ccggtgcgcg gcgcgacctg tcagggctac aaggatgaca gctgctgggc
     9781 gcgcgggcag gcgtggggcg tatacggcac ggccatcagc taccgctata ccaagcgccc
     9841 ggagtatctg gaggccttcc gccgggtgct gtcctattac cttgaccgcc tgcccgagga
     9901 tctgatccct tactgggata tgacctttac cgagggcgac accgagccgc gggactcctc
     9961 ctcggcatcc atcgtggcct gcggcctgct ggaggcggcc aagtacgcca accccgatca
    10021 ggctgcggag tacacgctgc tggcggccca gatgctgggc agcgtggccg agcattatgc
    10081 cgtcaggcag gggccgcagg gcatcggcct tgtgcgccac ggcacctaca gcaaaaagag
    10141 cccctacaac acctgcacgc cggagggcgt ggacgagtgc gtgagctggg gcgattactt
    10201 ctacatggaa ggtctgaccc ggctgacgaa ggattggacc ctttactggt gacatagggg
    10261 ccgggtgtgc cggcctctac aagaggtgat cctatgaact tacagacaaa acaagacttc
    10321 accgcccttg caatgcggtt tctcagcccc cttctgccgc tctattcccc gggcggggcg
    10381 ctgctgcatc tgggcgatac cggcgccacc tacccgcagc gcaccatcgg gatggaggct
    10441 ttctcccgcc cgctgtgggc gctggcaccg ctctgggcgg gcggcggcag ggccgatgcg
    10501 ttcctgccga tctaccagag ggggcttgtc aacggcaccg acccagacag ccccgaatac
    10561 tggggcgacc ccaacgatta cgaccagctt tttgtcgaga tggcggcgct ggcctgcgct
    10621 atactggaga cacccgagag tgtctggaac ccgctgacgg acaccgaaaa ggcaaagctg
    10681 ggcaagtggc tcgacacgat caatcatcac gacctgcccg gctgcaactg gctgctcttc
    10741 cgggtgctgg tcaatctggc gctggattcc gtgggtatgc cctgcagtat gcagcaggcc
    10801 gccgccgaca tggccgaggt ggacaagtgg tatgtcgggg acggctggta ctccgacggg
    10861 ccggccgaag tcaagccgca gaaggactac tataaccctt gggccataca gtattacacg
    10921 gtgctgtaca gcgtgtttgc cgcccggcgc gaccccgccc gcgcagcgct ctaccgtgag
    10981 cgcgccgtgc agttcggccg gcagttcgcc caatggtttg acccggacgg cgcggcgctg
    11041 ccctttggcc gcagcctcgg ctaccgcatc gggcagagcg cgttctacgc cgcctgcatc
    11101 tgggcggggc tggagccgct gcccctgccc gtcatgaagg gcatcatcgt ccgcaatctg
    11161 gcgtggtggc tggcccggcc catctttgac cgggacggtg tgctgacgat cggctacggc
    11221 tacccgcaga tgtacatggc cgagcagtac aacgcccccg gcagcccgta ctggggcctc
    11281 aaggtctttt tgctgctcgc tttgcccgac gatcatccgt tctgggcctg cgaggcagcc
    11341 cccctgccgg aggaactgac ccgtcccggc gtgatccccc agccaagcgc cgacctgctg
    11401 ctgcagcgcc tgccggacgg ccagttgaac gcctattccc cggcaaatta tgaaaagggc
    11461 gaccacggcc aatttaccga aaagtacggc aagttcgtct acagcacgcg gttcggtttt
    11521 tcggccagcc gcagctatgt gcagctggag cagaccgcgc ccgacagcat gctggccttt
    11581 gtcatagacg gctggacctt tgtacggcgg cacagcgaca agtttgtcct tttgggggat
    11641 cggctgctga gcgagtggcg gcccttcccg ggcatcaagg tcacgaccga gctggtcccc
    11701 accgcgtggg ggcacacccg cacccatacg gtcgagagca acatcgcctg cacggcctac
    11761 gactgcggct ttgccgtgcc gaagttcgcc gccggctttg cacagtcggc ggccggcagt
    11821 gaggccgagg cgaaaaacgc cgcctgccgc tgcgtggtaa aaggcgcagc agggcagggg
    11881 gtcgtgatca acgccgcccc caacacgaat ctgtacgacc ccaacacggt catccccgcc
    11941 gtgcggtatg agatacccat cggcaccgca gtgctgaaca cccgggtgga aagccggcat
    12001 aactaaaagc ttgggccgct gccgtgcagg gaccatcata aagcttttgc aaaaggcgaa
    12061 tcatcgctgc tttttgtatc atagagagag ggttttacta tgtatccgca aaccaatgct
    12121 gtccgcagcg tccgggatct ttccggcgtg tgggatttcc gtttcaatgc caatgagcca
    12181 tggcagccca tcgcggtgcc tgcgtcctac aacgaccaga gccccgaccc tgagttccgc
    12241 cgccactacg gcatggccta ctaccgcacc accttcaccg tgcccgaggg cgcgcgccgt
    12301 gtgctgcggt ttgatgccgt gacccacaat gccgttgtgc ggctcaacgg tcaggagatc
    12361 gtcacccatc gcggcggttt cctgcccttt gaggccgata tcacggcgct ggccaagccc
    12421 ggcgacaccg tgacccttga ggtcgaggtc gataaccgga tcggccacgg cacgctgccg
    12481 gtcggcaatg agggcggcac tgccttcttc ggctccgaca atgccggtat cccggcagtc
    12541 gaggccggca aggcccgcca gcagatgcag ggtgtcaacc tgccgaactt cgacttcttc
    12601 aactatgcgg gcatcacccg ccccgtccgc ctgtacacga ccccggcggc ttatatccgc
    12661 gacatcacgc tggtggccgg tgctgacggc aagctgcact acaccgtgga cacagtgggt
    12721 gagggcaatg tgcagctgac cgtgctggac gccaacggcc gcgctgttgc cgatgcggac
    12781 ggtgctgcgg gtacgctgca ggtcaaaaac gcccgcctgt ggcagccccg ccccggcacg
    12841 ccctacctgt acacagcgct ggtgcggttc ggcaccgatg aataccgcca gccgttcggc
    12901 ttccgcagtg tcgaggtgcg cgggcatcag ttcctgatca acggcgagcc gttttatttc
    12961 aaaggcccct gcaagcatga ggattcttta ttccgcggcc gcgggtatga cgcctgcgtg
    13021 accgtgaccg atctcaagct ttatgagtgg ctcaacgcca actgcctgcg gatgagccat
    13081 tacccctacg ccgaggaggt ctacgacctc tgcgaccggc tgggcattgt ggttattgat
    13141 gagacgccgg ctgtcggcat cggcgcgggt gcggcgtgcg acccgtacaa gaccttcccg
    13201 ctgaaaaact accacagcgc cgtgctgaag gccatgatcg accgcgacaa aaaccatccc
    13261 tgcgtggtgc tgtggagcct tggcaacgaa ccggacaccg agcatttccc cgagagtgcc
    13321 cgcgattact ggcgcccgct gtatgagcag gcccacgcgc aggacccgca gggccgcccc
    13381 gtcacgatgg tctgctgcca gaacgactac accaaggaca tcacgacccg cacgatggat
    13441 gtcgtctgca tcaaccgcta ctacggctgg tataatttaa gcggcgatct cgacaatgcg
    13501 tcctacgcgt tccggcagga gctggacttc tggaagggga tcgacaagcc gctgatcctg
    13561 tccgagtacg gcgcggatac ggtcgcgggc ctgcacggcg ttgcccccga gatgttcacc
    13621 gaggaatttc aggtcgaata ctacaaggcc atcaacgcat gtctggacag ctacgccttt
    13681 gttgtgggcg aatggccgtg gaactttgca gacttctcga cccagcaggg cccgatgcgt
    13741 gtcggcagct gcaaccgcaa gggtctgttc acccgtgagc gcacccccaa gctggcggcg
    13801 cactatttcc gtgaccgctg ggcaaaaaag cagcccaatg accgataaaa gaggatgaca
    13861 ctatgacacc cgaactgaaa tggctgaccg acccgaccgt ctttgccgtg aaccggctgg
    13921 atgcacactc agaccatgtt tgctaccgca gcgaggcgga gatacgactg ggcggcagct
    13981 cgctgcgcca aagtctggac ggcagctggc gctttgccta cagcgcctgc cccgccgccc
    14041 gcccggcgga tttctaccgc gaggacgcgg atttctctgc tttcggcaca atctgcgtgc
    14101 ccgggcatat cgagctgcag ggctacgggc agatccagta caccaacacc ctctacccgt
    14161 gggacggccg cagctatctg cgcccgccgc agatcgactg ggacaacact cctgtcggca
    14221 gctatctgcg ggagtttgac ttggacgagg ggctgcgcgg gcagcgcgtc tgcgtcagtt
    14281 ttcagggtgc cgagcaggca ctgtatgtct ggtgcaacgg ccacttcgtc ggctacgccg
    14341 aggacagctt taccccctcg gaatttgacc tgacaccgta tatcaggcag actggcaacc
    14401 gcctgtgcgt cgaggtgtac aagcacagca gcgcctcctg gctggaggat caggactttt
    14461 tccgcttctc gggcctgttc cgctcggtct atctctacgc caagcccaag gtgcatatcc
    14521 gtgatatctg gctcaaggcg gagctggccg agggcaacac caccggcctt ctgaccccca
    14581 tcgtcaagct ggacggcgag acggacggcg cgcgggtaca cctgcgcctg accgacggcg
    14641 aggagttcgc cctctttgat gaggacattg acggcagcac catcgagctg agcgccatcc
    14701 acccgtggag ccatgagaca ccggtgctgt accatgcctt gctgaccctg tatgactccg
    14761 acggtgcctt gcaggaggtc ttgccctacg acatcggctt ccgccgcttt gagatgaagg
    14821 acggcatcat gtgcttaaac ggcagccgca tcatcttcaa cggtgtcaac cgccatgagt
    14881 ggaatcccga gaagggccgc gccattgacg agagtgatat gcacgccgcg atggccgtct
    14941 ttaagcgcaa caacatcaac gctgtgcgta cctgccacta ccccaaccag agccggtggt
    15001 atgacctctg cgataaaaac ggcatctata tgatagacga gaccaacctt gaaagccacg
    15061 gcagctggca gaagctgggt gtggtggagc cgagctggaa tgtccccggc agcctgcccg
    15121 aatggcggga ctgcgtggtc gatcgcgcgc gcagcatgtt tgagcgcgac aaaaaccaca
    15181 cggcagtgct gatctggtcc tgcggcaacg agagctatgc cggcgaggat atccgcgcga
    15241 tggcggcgtt tttccgggaa aatgatcccg gccgccttgt gcattacgag ggcgtgttcc
    15301 agtgccgtgc gtttgacgac atcagcgata tggaaagccg gatgtacgcc agccccgcct
    15361 ccatcaggga atatctggac gccgacccca agaagccgtt tatcctctgc gagtatatgc
    15421 acgacatggg caactcgctc ggcgggatgg aaagctacat tgcgctgatc gaccggtacg
    15481 aaaagtatca gggcggcttc atctgggact acatggatca ggcgctctgg cataccgatg
    15541 caatgggccg ccgcgtactg ggctacggcg gagattttaa cgagcgcacg accgattaca
    15601 acttcagcgg caacggcatc gtctatgcgg acggcaccga aaagcctgcc atgcaggagg
    15661 tccgctactg gtacgacacc ccagaacgcc gcgctgtgca cgatgcgcac aataagctgg
    15721 cagccgagcg cagtgccgcc gcactggccg ccgcatggca aaagcgccca acccggcccc
    15781 tgaccgtcac cgagggcgac ggcaacatag gcgtaaaggg cagcggcttt gaagtgctgt
    15841 tctccatttc cgagcagggt cccgtttcgc tgcgcaaaaa cggcgccgag tggctgtggc
    15901 gcgccccgcg cccggcattc tggcgagcct ctaccgacaa cgaccgcggc tgcagtttcc
    15961 cgcagcgcgc cgccgtctgg atgggtgccg acgtctttgt gcagagaatg ggctttaccg
    16021 tgcaggaaaa atccgcgcag tgtgtgcggg tgcagtaccg ttttggtgtg cccggcgtac
    16081 cgggcgcgca ggtcgagatg atctacacgg tcgaagcaca gggtgcgctg cggctggatg
    16141 ccgtttacca cggcgtaccc ggcgcaccgg agctgccctg cttcggcatc aagtttgaaa
    16201 cggctgctcc cgttgtgcgc acccgttgga cggggctttc aggcgaaacc taccccgacc
    16261 gctgcaaggg cggggtgttc ggctgccacg aggaaccccc gcacattgaa ccggcgctgg
    16321 tgccgcagga ttgcggtttg cacatggata cccagcaggt aaacctgcag ttggctgacg
    16381 gcaagaccct gacgttggaa tccaccggcg aggcgtttgc cttcagcgcg ctgcccaaca
    16441 cggcgcagca gatcgaggct gcgcagcacc cctgtgagct gcccgaaacc gggcgcacct
    16501 gcgttaccgt gctgggcgca gcgcgcggcg tcggcggcat tgacagctgg ggtaccgatg
    16561 tggaggcacc ataccatgtc aacggcgaac agcagcacag cgtcagcctg aggatcctgc
    16621 tgtaataaaa tagcaaagct catagaaaat ctgagggtct gctctgaaaa gcgcccggac
    16681 ggctgaattt ttcaaccgtc cgggcgcttt ttgtggataa aatttgcgaa gttggcaggt
    16741 aaaaatagga gcggtcagtg tagtcagctc gctttgacga gtaccagcac gctgttggtt
    16801 ggcactacga tttggtcttg tttggtggga atgggtgggt tcttatattg gtgtagggct
    16861 ttttggattt gctgtttgac ctccagcagg gcggcgtcta gtgttggcgc ttgtgcggtg
    16921 attttgggga agtccggcac atgggcagtg cagccgtccg gggatagtgt gcaaagcacc
    16981 ggataaggct ggttctccac agagatactg tactgcatcc cgaacatgtc atccatcttg
    17041 tccatttcgt gccgcttatg ttcgtccatg ctgtggacat aggtgtccat tgtgaatgct
    17101 acggagtaat gaccgaggat ggccgacagc gttttgtagt ccatgccgcg ctccagcgca
    17161 cgggtggcga aggtgtggcg cagggcgtgg aaggtaaagt gtccgatgtc ggcatccttc
    17221 aacaggcgat cgtagtaatc cttgaaagtt ttctgctcaa agtagtgacc gaattcgttg
    17281 gtaacgatga agccatagtc ggtgtactta tcccctgccc gaatcttatc ctgcgcttgc
    17341 tgttgtttcc agcgggcaag ctcgtctgcc attgtgcggg tcagcggaat cgtgcggcga
    17401 gagtttttcg ttttgggtgt accgaatacg atttcagttt tgttttcgcc gtcgtgggct
    17461 tcgtacttgg caaggcggtt aatcgtgcgc ctgacatgaa gctgcgcggt ggaaaagtcg
    17521 atgtcctccc actttagtgc cagcaactcc cccatgcgta atccggtaca caagtctaag
    17581 cggataaaca cgccgtagcg gtggcggtag ctggcctgca ccagcgcccg ctgctggtcg
    17641 ttggtaaaga ctacgacttc gggtttctct ccgctgggca gggtcacggc atcgcaggga
    17701 ttataaacca gtagatgctc ttttacagct tgttgtaagc acttgtgtaa tgccatgtgg
    17761 tagttgcgaa gcgtcttggg cgacagccct tcttcgcgga atttgtagtt ataaaattcc
    17821 tgcagggtat gaggttcaag cttcttgagc aggatttttc ccagtacaca aaagtgcttg
    17881 ttgagataac tgcggtagct ggcgtaggtg gactgcttga cggtatcttt catgtaagtc
    17941 tccagccagt attcgtacca ttcgccaaga gtcaccgaca taggatcgcg ccagccctgt
    18001 gtgcggactt cgtgggcttg ctgctgcagg attgcgattg cctcttgccg cgtgttgcag
    18061 caggtggatg tgcgtctggg cttaccggtt ttgtagtcga ttccggcgga tagccgcacc
    18121 tcccaccgcc cgtctgttcg ctgacggatg ttgccctcgt tatgcccgcg gcgcagggat
    18181 gttttagatg tgtctttcac ggtgcatcgt ctcctttcgt gacacaaaga ctaccacagg
    18241 tacgaccaca aagctattca tcagacccga caatcttcgg gttctcgttt tgggctttat
    18301 tgaccttttg ctgctccagc cagcccagaa gttcatcccg ccgtaccagc agtcggtttc
    18361 caatgtggaa cgccgggaaa ctcgcgtcgt gaaatatgcg gtaggtggtc gagcg
//
LOCUS       DBMDSQ010000008        18311 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_995_length_18311_cov_2.351345, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000008 DBMDSQ010000000
VERSION     DBMDSQ010000008.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 18311)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 18311)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18311
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_995_length_18311_cov_2.351345"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..799
                     /locus_tag="ACZPAX_00615"
     CDS             <1..799
                     /locus_tag="ACZPAX_00615"
                     /inference="COORDINATES: protein motif:HMM:NF037639.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HIR1112431.1"
                     /translation="ITITYTATVQKDAIVAGSGNVNSAKLQYSRTTDTTKAEEGENEP
                     YEIEDQSVVYTFSTSLLKKGEGGTYDGTAMPGVTFALYKKVENNEWSDQDKVTIAGKE
                     YNKNTDVISNAVALGLTENEEETWIKIPYADRAEDGSTDTNGKLTYSGLPKGTYRFVE
                     TKTLDGYNLLSEPVDAKLNQQYSTVWTTSTTYVGGKLEKNKYSSTTYTGDAASTTDKP
                     ITIINRSGFQLPVTGGFGTLLFSGIGVLLVLAGVAVLFSMKKKNDRA"
     gene            1145..2023
                     /locus_tag="ACZPAX_00620"
     CDS             1145..2023
                     /locus_tag="ACZPAX_00620"
                     /inference="COORDINATES: protein motif:HMM:NF033745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HIR1112432.1"
                     /translation="MDTTGKLKLGFAALMILSGIGVAMYPVVSNAVAQRHASAVIDSY
                     DDTVQSMDTEKLDAAKEAARSYNEQLSNAVGRDAAGEAADTGTSYVDLMDVGESLGYI
                     KIPKIDVNLPIYEGTSDEVLLKGVGHLEGSSYPLGGDSTHSVLTGHRGLAEAVLFTDL
                     DKMQEGDKFYLHVMDEVLAYQVDQVKVVEPEHTEDLEIIPGGDYCTLVTCTPYAINTH
                     RMLVRGVRVPYNGEDEDADTPQAVQYQALNTGNIVKRVVDAWPWISVTGSIIIGGEAL
                     LLLLLLHRCKKREEED"
     gene            2023..3027
                     /locus_tag="ACZPAX_00625"
     CDS             2023..3027
                     /locus_tag="ACZPAX_00625"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:NF033745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HIR1112433.1"
                     /translation="MKRLLLQIFAWLAILVGAGLMLQPTVSQYLTRRKSTAAVETFVQ
                     SAAESAAVAADDPAAENTRPYADLYDALQTYNAEIYAAGQSGLKDPFAYETPAFDLTA
                     YGLPDDMIAALWVPRLDLDLPVYLGASTENMARGGALLGQTSMPLGGANTNTVIAAHR
                     GYYGAEMLRNVQQIQIGDKITLTTPWETLTYRVCELKIIQPDDINAILIQPGRDLLTL
                     TTCHPYTQNTQRYLVIAERDPDAAPATHEEDLAECAGSWDAAPRQVTVESPDGTAAVE
                     EIAPESISPLPNEGSGEDAGGAYSNWMIWLENNALWAGIVLIAAVVGCGLILRRHRKD
                     "
     gene            3031..3987
                     /locus_tag="ACZPAX_00630"
     CDS             3031..3987
                     /locus_tag="ACZPAX_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007782011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR1112434.1"
                     /translation="MQTREKALAVTAEVGKAVLGKPEAILKIMMAMLARGHVLVEDIP
                     GVGKTTLAQAFARAMQLTQNRVQFTPDVLPSDIVGFSLYQKETGKFVYHPGAVACNLF
                     LADEINRTSARTQSALLEVMEEGAVTVDGVTRKLPQPFTVMATENPVGSIGTQMLPES
                     QLDRFMVCVVMGYPDTEAEMEILTRQPRHGLLDRVQPVMDQNDLLTMQAEVDNIYMSE
                     EILRYIVELSQATRKDARLALGLSPRASLAVAGMARAAAYLSGREFVVPQDVTAIFAD
                     TAHHRLSLTEQARTGGETVENVIADVLHTVPRPRPEKPTHGR"
     gene            3977..6898
                     /locus_tag="ACZPAX_00635"
     CDS             3977..6898
                     /locus_tag="ACZPAX_00635"
                     /inference="COORDINATES: protein motif:HMM:NF013961.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HIR1112435.1"
                     /translation="MAGKVGCGLLLAVLFCAAAVSRAPFVVFLLALAMVFTAFLWWDA
                     RTLRRQMTAALQLPAKPVRPGEAFAVTVHLQNAGRRTVPELRAVLRAEDAESGTAIEL
                     PCAAMLAPGGRADLRVTLRAAKSGLWRFKLQKLTVRDHLGLFAADCPPDGQTWELCVL
                     PPAEGEADGAGNTPQNRAEQESRAGQDIADGVYDLRPYRVGDPLKQIHWKLTAKVDEL
                     TVREPLGTTYRADPAGTLLAEDGGTPPPVLHFGRAAVTRLRTLTRRKAADPDTGLEFV
                     DRVLLPQQGAAARPVLRGCVDFSILLLLTLGLLAAVNSAFSLALPAWIWPGAAALCAL
                     WLALCYAPLPAAAKRGAVLALAVGYLVLLFLVQRDFLRGMDAFANAVKLCLNTRFNAD
                     FSVGEAGACGLFVLLALVPITAFFAAVTVWRSDALLLDLVLLPAVVLVLLAGAGGGAV
                     GWLLLLLGCVGAWAAARSVRRRRLWGKKDSTVYTANVENFLTTQKTTAAGMMLVCAVL
                     FLPALALRPGLALQGLEDLLVTTDEKPTETVYLRGFIGAEYDGHSWQPADAEQFDNAA
                     ASWKTDGDSRLAIANLPFLRAAYDGAAPQQMTVQRLHAGDTYTFAPYNAYFNDYYRLD
                     GDGAAAGQTAQDDTFYYFPRGEAKTLLTARADADPSVLDRLESAYAAYAESRYLATPD
                     GADYGTLQEFCDAAEKEHKLTGDADAIRTFVRSYLNEHCTYTSDPPQPAEGADPVLYF
                     LNESHAGSSTQFASSAVVLCRMLGLPARYVVGYAAPQSLFAERDVGWQAVLQDDNAHA
                     WAEVYLAGQGWTPLETTPGMVSELTEDQLAANANAVPTADDAETPLPAATPAPEADPT
                     APQARAFWWLLPLAAVLAALAAWGIARARRTPADTVRAEFRALYKKMCRCGLGEGVSS
                     DQEEFKQFLWEHTKTAAPQDVETLLALVQTAQFAPGPVSAQTAQQVRGLCRILRKNLH
                     R"
     gene            7016..8032
                     /locus_tag="ACZPAX_00640"
     CDS             7016..8032
                     /locus_tag="ACZPAX_00640"
                     /inference="COORDINATES: protein motif:HMM:NF012742.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR1112436.1"
                     /translation="MARLPKNDKVVDIGTIAAALGVSKTTVSRAISGNGRVGAATRQR
                     VQDYVKEHNYTPSVMARGLAMRCSYNISLVISKQFSDFEMPFLRKTMRSVYQVAEDCD
                     YDVLLTLVGENETRPVERLLDNRKIDGLILTRTLERDPLIPLLKSRKLPFVAIGRPED
                     PTVMSVDHDQIGGCRELTSLLLMKGMKRIALLGGTMLYTVNMSRLEGFKQAYDMMNQK
                     IDESLLFLELETDDLRMQAVSQALERKPDCILCMDDRLTLLAMNMLKQQGVRVPQDIR
                     VASLYDSSALAESSPAITAVQFDAYALGRKATQQLLQVLKGREVETRVELGYQVSMRE
                     STKT"
     gene            8501..9871
                     /locus_tag="ACZPAX_00645"
     CDS             8501..9871
                     /locus_tag="ACZPAX_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013977853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR1112437.1"
                     /translation="MKAKKTLSVTLAAAMAAGLLAGCGGSASTATSTAEAASTADSTS
                     TAASTEAEAPAAAGKVYYLNFKPEQDEAWQDLAKKYTEETGVPVTVVTAASGQYETTL
                     MSEMAKSDAPTLFQVNGPVGLANWKDYCYDLSGTKIAGDLTSDTYALKDGDQMLGIGY
                     VIESYGLITNKTLLEKAGYTVDDIKSFDDLKKVAEDITARKDELGFSAFTSAGMDGSS
                     DWRYKTHLANLPIYFEYQEDGIDNTDAIKGTYLDNYKNIFDLYINNSTCDPTELAGKT
                     GDDSRNEFLNEEAVFYQNGSWEYTNLVGDGKPFTDDDLTMLPIYIGVGDEANQGLCTG
                     TENYWCVNKEASEDDINATLDFMYWCVSSDEGTKAMANDMGFVIPFKNAVESPNVFVK
                     ADKEMTAAGKTPVAWNFSTMPSENWKNGVGSALTAYAAGTGSWDDVVTAFVDGWASEA
                     ALNAAA"
     gene            10069..10920
                     /locus_tag="ACZPAX_00650"
     CDS             10069..10920
                     /locus_tag="ACZPAX_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004054335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR1112438.1"
                     /translation="MEKAIKRYWPVFVLPTLIAFIIGFVWPFIWGTGLSFCKFTTVKN
                     VQFAGLSNYKMILMDDTFTHAFWFTAAFTVVSTVLVNVLAFAIALLLTRGMRGTNAFR
                     TIFFLPNLIGGIVLGYIWQILLNGLLSAWGKSLLALNATYGFWGLIVLMCWQQIGYMM
                     IIYIAGLQNVPTDLIEAARIDGATSSQILFKIKVPMVMPSVTICTFLTLTNSFKLFDQ
                     NLSLTAGEPAKQSMMLAYNIYDTFYARSGPQWKGIGQAKAVVFFLFVVVISLLQLHFT
                     RSKEVQA"
     gene            10920..11768
                     /locus_tag="ACZPAX_00655"
     CDS             10920..11768
                     /locus_tag="ACZPAX_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004603665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR1112439.1"
                     /translation="MGATKKDRRINAVLSVLLTILCIAWMYPVFMILMNSLKTERAIT
                     TTHAFELPNAETFVGLKNYIYGIQEMDFLSSFWYSLVITVSSVALILLCCSMCAWYIT
                     RVQGVLGKGMYYLCVFSMIVPFQMVMFTLAKTADTLKLNNPYNICIIYLGFGAGLAVF
                     MFTGFVKSMPIAIEEAAMIDGCNPLQIFFKVVCPILKPTMISTAILETMWVWNDYLLP
                     TLVLDIKTYRTIPMAIQYFRGSYGKVEMAPMMACIMITVLPVIVLYLLCQKYIIDGVV
                     AGAVKG"
     gene            11801..12805
                     /locus_tag="ACZPAX_00660"
     CDS             11801..12805
                     /locus_tag="ACZPAX_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009062892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR1112440.1"
                     /translation="MTIKDIARESGFAIGTVSRALNNAPGVSAETRQKILEVVNRHNY
                     EPNANAKHLKQQANDGIALIVKGTQNMLFADILEQLQSVIEESGYVATVYYIDEEANE
                     VAQARRICAERKPYGILFLGSNLEYFTPDLEDLGIPCLLLTNSAATLRIANLSSVSVD
                     DMAASDRMIEYLYKQGHRRIGIIGGKPNLSRPSLARLAGCQSAMLRLGLPFEMEKQYA
                     YARFSLRSGYEAMEYLLDHSPDITTVFAMSDLMALGAIRALQDHGLRVPQDISVAGYD
                     GIALGQYSIPRLTTIRQDMEQLAQRSAKILLHCIEEPGRAVHEVVPFDLIEGESVGKV
                     "
     gene            13034..14515
                     /gene="malQ"
                     /locus_tag="ACZPAX_00665"
     CDS             13034..14515
                     /gene="malQ"
                     /locus_tag="ACZPAX_00665"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007048709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HIR1112441.1"
                     /translation="MRASGILMPISSLPSAHGIGAMGAEARRFVEFLSKAGQAYWQIL
                     PICPTSYGDSPYQSFSTFAGNPYFIDLDELAKRDLLLPEEYENIDWESAPDTINYGAL
                     YEKRYKVLRCAANRLLAKPNAAYRKFVKENAFWLPDYALFMALKDAHGGACWLEWEEP
                     LRRREPEALKAAREQYADDVAFWQVVQYLFYAQWNALKGYANRMKIDIIGDLPIYVAL
                     DSVDVWSCPQEFQLDENLLPTEVAGCPPDGFSATGQLWGNPLFDWDAMAKTGYAWWVR
                     RIRHMCSIYDVVRIDHFRGFAGYYAIPYGDKTAEHGRWREGPGYALFAAIKKKLGSPR
                     IIAEDLGFLTEDVNALLKECGYPGMKVLEFAFDSRDGGDYRPHSYQNNCIAYVGTHDN
                     EPVNGWMETAAPEDVERAVRYLNLTKKEGYHWGMMRGVWSSVADLAVVQAQDVLGLGH
                     ESRMNTPSTLGGNWCWRAEPRVFNSRLARRLHDLMELYGRLPE"
     gene            14679..15854
                     /locus_tag="ACZPAX_00670"
     CDS             14679..15854
                     /locus_tag="ACZPAX_00670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1112442.1"
                     /translation="MKVCQKCHTPARYDTQKFCSVCGTPFPDMTPAEPAAPARQPAQP
                     VQPAPQAPVQPPVQPPVQPYAQQPQNPFGAAAPQPEQPEQPKKSKLSIIIAAVVVVVV
                     VAAAAAVFFLRGSKDNAATLPAAEPVATEAADAAAPQTPAPADSAAAAQTPAPAVTPQ
                     PAQTPAPAAAVATDTQVVLRNAAANGTITVDGKAVDFAYVGNDAVIPRSSLSDVCQVR
                     IVADNGNGGYKTAAVWYNKDYGNELTFGADYGAYTDCDATGLAKPGDKVVDVLTWAFY
                     RSFQEAINEGNSSLLRYSTAGNSTRCAAEIRNYTGNTYDLGNFQAVSDPASIKYTDGQ
                     VIYNGHFVANYTDSGSSYTADHHRTLRLVWEDGIWKVDAFSLLPDGAYSATTYATLP"
     gene            15875..16897
                     /locus_tag="ACZPAX_00675"
     CDS             15875..16897
                     /locus_tag="ACZPAX_00675"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024109.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HIR1112443.1"
                     /translation="MKNTKLKLAAASLALLLAGCGASATVEPQPTPAAALAPTAAPAV
                     TEAPAATPEPIPPESYDQRDETFRARHDGTEVTVHATVTTPAEGAGWPLVVLCHGFTG
                     SRGGDGHFGPLAARLAHAGIASIAMDFPGCGESEEPFTAYTLTNIRADMTAAIAYMGV
                     TYGADTTRLGLVGHSMGGRAVSLYLDGRDYPVTAAALWSPADNTGLDGLEFLAHDADE
                     RAALRQTADENGSVELPQWGVAAGAALFDEMAASDPCGVLAEYPGALLAAFTGGDAEL
                     LSEDTVALVQNTLATRDLPFVDMTNDFPDANHNYQAAEGSAASDAEIAARIENATAEF
                     LVSALR"
     gene            complement(16901..17548)
                     /locus_tag="ACZPAX_00680"
     CDS             complement(16901..17548)
                     /locus_tag="ACZPAX_00680"
                     /inference="COORDINATES: protein motif:HMM:NF015431.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HIR1112444.1"
                     /translation="MGRGGGLVLLGGIRKLAAQTVPDLMQKFLATSGNEHTRFELHTE
                     SSFTADLLQAVAEERLDMAFVSHPGDDTVFENVAFARSPFVVVTPPNHPLAQKNSVTL
                     RETLPYPFVYFSKRGGLRRLIDALFQKIGATPKIAYETEEDTVVAGMAAAGFGIAIVP
                     DHPVLRCMDLKIIPLTDPDPGRTAYLCRKRGALQPAAAQRFFAFCQAQLSQGASL"
     gene            17576..17860
                     /locus_tag="ACZPAX_00685"
     CDS             17576..17860
                     /locus_tag="ACZPAX_00685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1112445.1"
                     /translation="MAGAGAGTIYPACLPTALKRFPDKAGSISSLVQAGASCGPFIMS
                     PIAQWLIDTYGAQMACRILGRPMRTAAPAREMENPPTTYLHCPEIVLYYF"
     gene            17896..>18311
                     /locus_tag="ACZPAX_00690"
     CDS             17896..>18311
                     /locus_tag="ACZPAX_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005360849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR1112446.1"
                     /translation="MPKYSVKKPFTILVAVILVLVLGFVSLTGIQTDLLPAMNLPYLL
                     VMTTYPGASPEQVEADVTQPLENALSVLNGVKNVTSQSNENYSLIFLEYQDDTDMDSA
                     MVKTSTAVNQLGDALPDMASTPTLMEISPDMMATQYV"
BASE COUNT         3722 a         5609 c         5590 g         3390 t
ORIGIN      
        1 gattaccatt acctatactg ctactgtaca gaaagatgct attgttgctg gtagcggtaa
       61 cgtgaacagc gcaaagttgc agtactcccg cacgacggat acgactaaag cggaagaagg
      121 cgaaaatgag ccttacgaaa tcgaggacca gagcgttgtt tataccttct cgaccagcct
      181 tctgaaaaaa ggcgaaggcg gtacttacga cggtacggcc atgccgggcg ttacctttgc
      241 gctgtataaa aaagtggaaa ataatgaatg gagcgaccaa gataaagtta caatcgcagg
      301 caaggaatac aataaaaaca ccgatgtgat tagcaatgca gttgcacttg gactgacaga
      361 aaatgaggaa gaaacgtgga ttaaaattcc ctatgcagat agagcggaag atggttctac
      421 ggatactaac ggcaaactga cctactctgg cctgccgaaa ggtacttaca gatttgttga
      481 aaccaagacg ctggatggtt acaacctgct gtccgagcct gttgatgcaa agttgaatca
      541 gcagtactcc actgtgtgga ctacgagcac gacctatgta ggcggaaaac tggaaaagaa
      601 caagtactcg agtacaacct atactgggga tgcggcatct actacggata aacccattac
      661 catcatcaac cgcagcggct tccagctgcc tgtcaccggt ggttttggca cactcctgtt
      721 cagcggtatc ggcgtgctgc tggtgctggc cggtgtggcg gtgctgttca gcatgaaaaa
      781 gaagaacgac cgcgcttaaa accgcatgat gcaaacacca aaagataatt agaagggtaa
      841 acccataggg gaagggccgg gattggctct tcccctatgg tggttatggg ggtataatat
      901 aaggcaaagg ggatatggcg gggagaaatc cctccggccg ccgatggcgg ccacctccct
      961 ttgacaaggg aggcagaatc cctccggccg ccgatggcgg ccaccgctcc ccttccggcg
     1021 cttcgcgcca cctcccccat cccgggggag tctgtctttg acaagggcgg cttgcgggcc
     1081 gatgcaggca tcggcccctg caaattgcca cagatatttt ccccatgaac agaaaggcac
     1141 acctatggat accacaggca aattaaaact tggatttgcg gcgctgatga ttctgtcggg
     1201 catcggcgtg gcgatgtacc cggtcgtcag caacgcggtg gcacagcgcc atgcgtcggc
     1261 ggtcattgac agctacgacg ataccgtcca atccatggat acagaaaagc tcgatgctgc
     1321 aaaagaagcg gcccgcagct acaacgaaca gctcagcaat gccgtgggcc gcgacgcggc
     1381 gggcgaagcc gccgataccg gcacaagcta tgtggacctg atggacgtgg gcgagagcct
     1441 gggctacata aaaatcccca aaatcgacgt caatctgccg atttatgagg gcaccagcga
     1501 cgaggtgctg ctcaagggcg tcggccatct ggaagggtcg agctatccgc ttggcggcga
     1561 cagcacccac agcgtgctga caggccaccg cggcctggcc gaggccgtcc tgtttaccga
     1621 tttggacaaa atgcaggaag gtgacaaatt ttacctgcat gtcatggacg aagtcctagc
     1681 ctaccaggtt gaccaggtca aggtcgtgga gccggaacac accgaggatt tggaaatcat
     1741 ccccggcggg gactactgca cccttgtcac ctgcacgccc tatgcaatca acacccaccg
     1801 tatgctggtg cgcggcgtgc gcgtgcccta taacggcgag gacgaggacg ccgacacccc
     1861 gcaggccgtg cagtaccagg cattgaacac cggcaacatc gtcaagcgcg tggtggacgc
     1921 ctggccgtgg atcagcgtga ccggcagtat tatcatcggc ggcgaggcac tgctgctgct
     1981 tttgctgctg caccgctgca aaaagcgcga ggaggaagac tgatgaaacg cctgctgctg
     2041 caaatttttg cgtggcttgc catactggtg ggcgcggggc tgatgctgca gccgacggtc
     2101 agccaatacc tgacccgccg caaaagcacc gccgccgtgg aaacctttgt gcagtctgcc
     2161 gccgagtccg ccgccgtggc tgctgacgac ccggctgccg aaaacacgcg cccctacgca
     2221 gatttatacg acgcactgca aacctacaac gcggaaatct acgccgcggg gcaaagcggg
     2281 ctgaaggacc cctttgccta cgagacgccc gcctttgacc tgacggccta cggcctgccc
     2341 gatgatatga tcgccgcgct gtgggtgccg cgcctggatt tagatttgcc ggtgtatctg
     2401 ggggccagca ccgaaaacat ggcccggggc ggcgcactgc tggggcagac ctcgatgccg
     2461 ctgggcggcg cgaacaccaa cacggtcatt gccgcccacc gcggctacta cggcgcggag
     2521 atgctgcgca acgtgcagca gatacagatc ggcgacaaaa tcaccctgac cacgccgtgg
     2581 gagactctga cctaccgcgt gtgtgaactg aaaattatac agccggacga cataaatgcg
     2641 atactgatac agccgggacg ggatctgttg accttgacga cctgccaccc ctacacccag
     2701 aacacccagc gctacctggt cattgccgag cgcgaccccg acgccgcccc cgccacccat
     2761 gaggaagatt tggccgagtg cgcgggcagc tgggacgccg cgccccgcca ggtgacagtg
     2821 gaaagccccg acggcaccgc cgccgtggag gaaatcgcgc ccgagagcat cagcccgctg
     2881 ccgaacgagg gcagcggcga ggatgcgggc ggggcgtact ccaactggat gatctggctg
     2941 gaaaacaacg ccctgtgggc aggaattgtg ctgattgccg ccgtggtggg atgcgggctg
     3001 atcctgcgcc gccacagaaa ggactgaccg atgcagacac gagaaaaagc gctggccgtg
     3061 acggccgagg tgggcaaggc cgtactgggc aagcccgaag caattttgaa aatcatgatg
     3121 gcaatgctgg cccgcggcca tgtgctggtg gaggatatcc ccggtgtggg caaaaccaca
     3181 ctggcccagg cgtttgcccg cgctatgcag ctgacccaga accgcgtaca gttcacaccg
     3241 gacgtattac cgtctgatat tgtggggttt tcgctgtacc aaaaagagac cggcaagttc
     3301 gtttaccacc ccggcgcggt ggcctgcaac ctgtttttgg ccgatgagat caaccgtacc
     3361 agcgcccgca cccaatccgc cctgctggaa gtcatggagg aaggtgcggt gacggtggac
     3421 ggcgtgaccc gcaagctgcc ccagcccttc accgtcatgg cgacggaaaa cccggttggc
     3481 tcgatcggta cgcagatgct gccggagtcg cagctcgacc gttttatggt ctgcgtggtc
     3541 atgggctacc ccgacaccga ggccgaaatg gagattttga cccgccagcc gcgccacggt
     3601 ctgctggacc gcgtgcagcc ggtcatggac cagaacgacc tgctgaccat gcaggccgag
     3661 gtggacaaca tttatatgag cgaggaaatt ttgcggtata tcgtagagct ttcccaggcg
     3721 acccgcaagg acgcacggct ggcgctgggg cttagccccc gtgcgtcgct ggccgtggcc
     3781 ggtatggcgc gggctgcggc gtacctctcg gggcgggagt ttgtcgtgcc ccaggatgtg
     3841 accgcgattt ttgccgacac cgcgcaccac cgcctgagcc tgaccgagca ggcccgcacc
     3901 ggcggggaga cggtggaaaa cgtgatcgcc gacgtgctgc acactgtgcc gcgcccccgc
     3961 ccggagaaac cgacccatgg caggtaaggt gggctgcggc ctgctgctgg ccgtgctgtt
     4021 ttgcgccgcc gcggtcagcc gtgcgccgtt tgtggtgttc ctgctggcgc tggcgatggt
     4081 gttcaccgcg tttttgtggt gggacgcccg cacgctgcgg cggcagatga cggcggcctt
     4141 gcagctgccc gccaaaccgg tgcgccccgg cgaggcattt gccgtgaccg tacacctgca
     4201 aaacgccggg cggcgcaccg tgcctgaact gcgggccgtg ctgcgggccg aggatgccga
     4261 gagcggtacg gccatcgagc taccctgtgc cgccatgctg gccccgggcg gccgcgccga
     4321 cctgcgggtg acgctgcggg ccgccaaaag cgggctgtgg cggttcaaac tgcaaaagct
     4381 gacggtgcgg gaccacttgg gcctgtttgc ggcggactgc ccgccggacg gccaaacgtg
     4441 ggagctgtgc gtgctgccgc ccgccgaggg agaggccgac ggcgcgggca ataccccgca
     4501 gaaccgcgcc gagcaggaga gccgcgcggg acaggacatt gccgacggcg tctacgacct
     4561 gcgcccctac cgtgtggggg atcccttaaa acaaatccac tggaaattga cggccaaggt
     4621 ggacgagctg accgtgcggg agccgctggg cacgacttac cgtgccgacc cggccggcac
     4681 gctgctggcc gaggatggcg gcaccccgcc gcccgtgctg cattttgggc gggcggctgt
     4741 gacccggctg cgcaccctga cccgccgcaa ggccgccgac ccggacaccg ggctggaatt
     4801 tgtggaccgc gtgctgctgc cccagcaggg tgcagcggca cggcctgttc tgcgtggctg
     4861 cgtggatttc tcgatacttt tgctgctgac gctggggctg ctggcggcgg tgaatagcgc
     4921 gttttcgctg gcgctgcccg cctggatctg gcccggcgcg gcggcgctgt gcgccttgtg
     4981 gctggcgctg tgctatgcgc cgctgcctgc cgctgccaag cgcggggctg tgctggcgct
     5041 ggccgtgggg tatctggtgc tgctgttttt ggtgcagcgg gacttcctgc gcggcatgga
     5101 tgcctttgcc aacgccgtga agctatgcct gaatacacgt ttcaacgcgg atttttccgt
     5161 gggtgaggcg ggtgcgtgcg ggctgtttgt gctgctggcg cttgtgccca tcacggcgtt
     5221 ttttgccgct gtgacggtct ggcgcagcga cgcgctgctt ttggatttgg tattgctgcc
     5281 tgccgtggtg cttgtgctgc tggcgggcgc gggcggcgga gctgtgggct ggctgctgct
     5341 gctcctgggc tgcgtcggcg cgtgggcggc ggcgcggtcg gtgcggcgca ggcggctgtg
     5401 gggcaaaaaa gattccaccg tttatactgc gaatgtggaa aacttcctca cgacccagaa
     5461 aaccaccgcc gccgggatga tgctggtctg cgcggtgctg ttcctgcctg cgctggcact
     5521 gcgcccgggg ctggccctgc aagggctgga ggatttactc gtcacgaccg acgagaagcc
     5581 gacggagacg gtctacctgc gcgggtttat cggcgcggaa tacgatgggc atagctggca
     5641 gcctgcggac gccgagcagt tcgacaatgc cgccgccagc tggaaaaccg acggtgacag
     5701 ccgtttggca atcgcgaacc tgccgtttct gcgggccgct tacgatggtg ccgcaccgca
     5761 gcagatgacc gtgcagcggc tgcacgccgg cgatacctac acttttgcac cctataacgc
     5821 ctattttaac gactactacc gcctggacgg cgacggcgcg gcagcaggcc agaccgcgca
     5881 ggacgacacg ttctactact tcccgcgcgg ggaggcaaaa acgctgctga ccgcccgtgc
     5941 cgacgcggat cccagtgtgc tggaccggct ggaaagcgcc tacgcggcct acgccgagag
     6001 ccgctacctg gcgacgcccg acggcgcaga ctacggcaca ctgcaagaat tttgcgatgc
     6061 agcggaaaaa gaacacaaac tgaccggcga cgccgacgcg atacgaacct ttgtgcgcag
     6121 ctacctgaac gagcactgca cctacaccag tgacccgccc cagccagccg aaggggctga
     6181 cccggtgttg tatttcctga acgagagtca tgcgggcagc agcacgcagt ttgcgtcgtc
     6241 ggcggtggtg ctctgccgga tgttgggcct gcccgcccgc tatgtggtgg gctatgctgc
     6301 cccacaaagc ctgtttgccg agcgtgacgt cggctggcag gccgttttgc aggacgacaa
     6361 cgcccacgcc tgggccgagg tgtaccttgc cgggcagggc tggaccccgc tggaaacgac
     6421 ccccggcatg gtcagcgaac tgaccgagga ccagctggcc gccaacgcga acgctgtgcc
     6481 gactgccgat gatgccgaga ccccgctgcc cgccgcaacg cccgcgccgg aggcggaccc
     6541 gaccgccccg caggcccgag ctttttggtg gctgctgccg ctggcggcgg tgctggccgc
     6601 gctggccgcg tggggcattg cccgtgcacg gcgcaccccg gccgatactg tgcgggcgga
     6661 gttccgcgcg ctgtacaaaa agatgtgccg ctgcggcctg ggcgaagggg tatcctctga
     6721 tcaggaagag tttaaacaat ttttatggga acacaccaaa acggcggccc cgcaggatgt
     6781 ggaaacgctg ctggcgcttg tgcagacggc gcagtttgcg ccggggccgg tcagcgcgca
     6841 aaccgcgcaa caggtacgcg gcttgtgccg catactgcgc aaaaatttac acaggtaatt
     6901 gccgggaaat atgtgatatt ctccaggggc gagaagtaac aagagcaaat aacgccttga
     6961 cgcggcgctt caaacctatt attatagtag tgaaatgcaa tagggagatg aaatcatggc
     7021 gcgattgccg aaaaatgaca aagttgtgga cattggaacg atcgctgcgg ctttgggagt
     7081 gtcaaaaaca acggtatccc gggcaatatc cggcaatggc cgtgtgggcg cagcaacccg
     7141 tcagcgcgta caggactacg tgaaggagca taactacacc cccagcgtga tggcacgcgg
     7201 gctggcgatg cgctgctcgt acaacatttc gctggtgatc tccaagcagt tctctgattt
     7261 tgagatgccg tttttgcgca agacgatgcg ttcggtctat caggtggcgg aggattgcga
     7321 ttacgatgtg ctgctgaccc tggtaggcga gaacgagaca cgcccagtgg agcgcctttt
     7381 ggacaaccgc aagatcgacg gcctgattct gacccgcacg ctggaacgcg acccgctgat
     7441 cccgctgctg aaaagccgca agctgccctt tgtagccatc ggccgccccg aggatccgac
     7501 cgtgatgtcg gtggatcatg accagatcgg cggctgccgg gagctgacca gcctgctgct
     7561 gatgaagggc atgaagcgga tcgccctgct gggcggcacg atgctgtaca ccgtcaatat
     7621 gtcgcgcctg gaaggtttca agcaggccta tgacatgatg aaccagaaaa tcgacgagag
     7681 cctgctgttt ttggagctgg aaaccgacga cttgcggatg caggccgtga gccaggcgtt
     7741 ggagcgcaag ccggactgta tcctgtgcat ggacgaccgg ctgaccctgc tggcgatgaa
     7801 tatgctcaag cagcagggtg tgcgggtgcc gcaggatatc cgcgtggcgt cgctgtatga
     7861 cagcagcgcg ctggctgagt cgtcgcccgc catcacggcg gtgcagtttg acgcctacgc
     7921 cctgggccgc aaagcgaccc agcagctttt gcaggtgctc aaaggccgtg aggtggagac
     7981 ccgcgtggag ctgggctacc aggtcagtat gcgggaatcg acgaaaacct gagatagggt
     8041 atataataag tatattaggc agcacgccgt ggaaacggtg cgctgccttt ttgtaggggc
     8101 ggcatctatg ccgcccctac aactccacaa aaaaatgaat cgcgtaatgg cggcaaacgc
     8161 accgggaggg atgaatccct ccccccacag ggggatagag tcgagaaaaa tagaagcgga
     8221 tggaggtgcg atttgccgtg aaaactgcgc aacatatgga aacggttgcg caacaaagga
     8281 accgtttccg acaaaaagcg acctgttttt tgaaatattt ccacaaaaac aggcattcta
     8341 atttgtgcaa ggttccaatc ttggataaaa tcaccaaaaa acgattgatt tttcgctatt
     8401 tatggtgtat tataatatca atccgaaacg gaaacgtttc cgaaaaagaa aacgcaatca
     8461 ctactgcaac tcttgttttt ctttagaagg aggaaaaaac atgaaagcga aaaagactct
     8521 ctccgtgacc ctggccgccg cgatggcagc cggtctgctg gcaggctgcg gcggcagcgc
     8581 ctccacggcc acctctaccg cagaggccgc ttctaccgcg gactccacct ccactgctgc
     8641 ttctaccgag gctgaggccc ccgcagcagc cggcaaggtc tactacctga acttcaagcc
     8701 cgagcaggat gaagcctggc aggatctggc caagaagtac accgaggaga ccggtgttcc
     8761 cgtcaccgtc gtgaccgccg cttccggcca gtatgagacc accctgatga gcgagatggc
     8821 caagagcgat gcgcccaccc tgttccaggt caacggccct gtcggcctgg ccaactggaa
     8881 ggactactgc tacgacctgt ccggcaccaa gatcgccggt gatctgacca gcgacaccta
     8941 cgccctgaaa gacggcgacc agatgctggg catcggttac gttatcgaga gctacggcct
     9001 gatcaccaat aagaccctgc tggagaaggc cggctacacc gttgacgaca tcaagagctt
     9061 cgacgacctg aagaaggtgg ccgaggacat caccgcccgc aaggacgagt tgggcttctc
     9121 cgccttcacc tccgctggta tggacggctc ctctgactgg cgctacaaga cccatctggc
     9181 caacctgccc atctacttcg agtatcagga ggacggcatc gacaacaccg acgccatcaa
     9241 gggcacctat ctcgacaact acaagaatat cttcgacctg tacatcaaca actccacctg
     9301 cgatcctact gaactggctg gcaagaccgg cgacgacagc cgcaacgagt tcctgaacga
     9361 agaggccgtt ttctaccaga acggttcctg ggagtacacc aacctcgtcg gcgacggcaa
     9421 gcccttcacc gatgacgatc tgaccatgct gcccatctac attggcgtcg gcgatgaagc
     9481 caaccagggc ctgtgcaccg gcaccgagaa ctactggtgc gtcaacaaag aagccagcga
     9541 ggacgacatc aacgcaacgc tcgacttcat gtactggtgc gtttcctctg acgagggtac
     9601 caaggccatg gctaacgaca tgggcttcgt tatccccttc aagaatgctg tcgagtcccc
     9661 caacgtcttc gtcaaggccg ataaggaaat gaccgccgcc ggcaagaccc ctgtggcctg
     9721 gaacttctcc accatgcctt ccgagaactg gaagaacggt gtcggctctg ctctgaccgc
     9781 ctacgccgcc ggtaccggca gctgggatga cgttgtcacc gcctttgtcg atggctgggc
     9841 ttctgaggct gccctgaacg cagctgcgta agtagtgggt acgcaactgt aacgcattac
     9901 aattctttgc aaacgttttt ctcctaaaac ctcctaaaca tttctcttaa actgttttct
     9961 ccagtttttc tccttatcct ctttcatcac cggtcagcgg ggcgggcgga ttcgcccgcc
    10021 ccgctccatt tttgcaacag gaaatctaaa caaagggggg gcttttccgt ggaaaaagcc
    10081 attaaacgct actggccagt ctttgtactg cccaccctca ttgcctttat catcggcttt
    10141 gtgtggcctt ttatctgggg caccgggctg tcgttctgca aattcacgac ggtcaaaaac
    10201 gtccagttcg cggggctgag caactacaaa atgattctta tggatgacac cttcacccat
    10261 gcattctggt tcacggcggc gttcaccgtt gtatccaccg tgctcgtcaa tgtgctggca
    10321 ttcgccattg cgctgctgct cacccgcggt atgcgcggta ccaacgcgtt ccgtaccatc
    10381 ttcttcctgc cgaacctcat cggcggcatc gtgctgggct acatctggca gatcctgctc
    10441 aacggcctgc tgtccgcctg gggcaagtcg ctgctggcgt tgaacgccac ctacggcttc
    10501 tggggcctta tcgtcctgat gtgctggcag cagatcggct acatgatgat catttacatc
    10561 gccggcttac agaacgtgcc caccgacctc atcgaggccg cacgcatcga cggtgcgacc
    10621 tccagccaga ttctctttaa aatcaaggtc cccatggtca tgcccagcgt gacgatttgc
    10681 acgttcctga cgctgaccaa ctctttcaag ctgtttgacc agaacctgtc cctgacggca
    10741 ggcgagcccg ccaaacagtc catgatgctg gcctacaaca tctacgatac cttctatgcc
    10801 cgctctggac cccagtggaa gggcatcggc caggcgaagg ccgtcgtatt cttcctgttt
    10861 gtggttgtca tctcgctgct gcagctgcat tttacccgct ctaaggaggt gcaggcataa
    10921 tgggtgctac caaaaaagac cgccgcatca atgcggtgct gtccgtcctg ctgaccatcc
    10981 tgtgcattgc ctggatgtac ccggtgttca tgatcctgat gaactcctta aagacagagc
    11041 gtgccatcac cacgacccac gcctttgaac tgccgaacgc cgaaaccttc gtcgggctga
    11101 aaaactacat ttacggcatt caggaaatgg actttttgtc ctctttctgg tatagcttag
    11161 tcatcacggt ctcgtctgtc gcactgattc tgctgtgctg ctcgatgtgc gcatggtata
    11221 tcacccgcgt gcagggcgtg ctcggcaagg ggatgtacta cctgtgcgtg ttcagcatga
    11281 tcgttccctt ccagatggtc atgttcacgc tggccaagac cgcggatact ttaaagctga
    11341 ataaccccta caacatctgc atcatctatc tgggcttcgg cgcgggcctg gcagtgttca
    11401 tgttcaccgg ctttgtcaag agcatgccca tcgccattga ggaggccgcc atgatcgacg
    11461 gctgcaatcc cctccagatc ttcttcaagg tcgtctgccc gattctgaag ccaacgatga
    11521 tctccaccgc catcttggaa acgatgtggg tctggaacga ctacctgctg cccacgttgg
    11581 tgctggatat caagacctac cgcaccatcc ccatggcgat ccagtatttc cgcggcagct
    11641 acggtaaggt ggaaatggcc cccatgatgg cctgcatcat gattacggtg ctgccggtca
    11701 tcgtgctgta cctgctctgc cagaaatata tcattgacgg cgttgtggcc ggtgcggtca
    11761 aagggtaaac tcagccgaac cagaaaagag gtccggtttt atgaccatca aagatattgc
    11821 gcgggaatcc ggctttgcca ttggtacggt gtcgcgggcg ctgaacaacg cccccggcgt
    11881 cagcgccgag acgcggcaga agatcctgga agtcgtgaac cgccacaact atgaacccaa
    11941 cgcaaacgca aagcatttaa aacagcaggc caatgacggc attgccctga ttgtcaaagg
    12001 cacgcagaat atgctgttcg ccgatatttt agagcagctg cagagcgtga ttgaagagag
    12061 cggctacgtg gcgaccgtct attacatcga cgaggaagcc aacgaggtcg cccaggcccg
    12121 gcgcatctgt gcggagcgta agccctacgg catcctgttt ctggggtcga acctggagta
    12181 tttcacccct gatctggaag atttgggcat cccctgcctg ctgctgacca acagcgcggc
    12241 cacgctgcgc atcgccaacc tttccagcgt gtcggtcgat gatatggcgg cgtccgaccg
    12301 gatgatcgag tacctgtaca agcagggcca tcggcgcatc ggcatcatcg gcggcaagcc
    12361 gaacctgtcc cgcccgagcc ttgcgcgtct ggcaggctgc cagagcgcca tgctgcggct
    12421 gggcctgccc tttgagatgg aaaagcagta cgcctacgca cggttctcgc tgcgcagcgg
    12481 gtacgaggcg atggagtatc tgctggacca cagccccgat atcacgacag tgttcgccat
    12541 gtcggacctg atggcgctgg gagccatccg tgctttgcag gatcacgggc tgcgggtgcc
    12601 gcaggatatc tcggtagccg ggtacgacgg cattgccctg gggcagtaca gcattccgcg
    12661 cctgacgacc atccggcagg atatggagca gctggcccag cgcagtgcaa aaattttgct
    12721 gcactgcatt gaagaacccg gccgcgccgt acacgaagtt gtcccctttg atttgatcga
    12781 aggggagagc gtgggcaagg tttgacgact ttgcaaaaac agcgccgcca tgtaggggcc
    12841 gcacatgtgc ggcccgcggc cttgccgtag gtgcgcgttt gccggaaggc tgagggccgg
    12901 gcatacccgg cccctgtggt gcttgttgca gggggcgatg cctggcatcg ccctgcgggc
    12961 ggcatcgttg caggggggcg gcgtccccgg cacccacccc gcgtttacat tcaaaagaaa
    13021 gaggttttct ctgatgagag caagcggcat tctgatgcct atttccagcc tgccctcggc
    13081 ccatggtatt ggcgctatgg gtgcggaggc acgccgattt gtggagttcc tctccaaggc
    13141 cgggcaggca tattggcaga tcttacccat ctgccccacc agctacggcg acagccccta
    13201 tcagtccttc tcgacgtttg cgggcaaccc gtattttatc gacctcgacg agcttgccaa
    13261 acgcgacctg cttctgcccg aggaatatga gaacatcgat tgggaatccg cccccgatac
    13321 catcaactac ggtgctctgt acgaaaaacg ttacaaggtg ctgcgctgcg cggcaaaccg
    13381 tttgctggca aagcccaacg ccgcctaccg caagttcgtg aaggaaaacg ccttctggct
    13441 gccggactac gcgctgttca tggcactgaa ggacgcccac ggcggggcct gttggcttga
    13501 gtgggaagag ccgctgcgcc gccgcgagcc ggaggccctg aaggccgccc gcgagcagta
    13561 cgccgacgat gtggctttct ggcaggtggt gcagtacctg ttctacgccc agtggaacgc
    13621 gctgaaaggc tacgccaacc gcatgaagat cgacattatc ggcgatctgc cgatttacgt
    13681 cgcgctggac agtgtcgatg tgtggagctg cccgcaggag ttccagctgg acgaaaacct
    13741 gctgcccacc gaggtggcag gctgcccgcc agacggcttc tcggccaccg gccagctgtg
    13801 gggcaacccg ttgtttgact gggacgccat ggccaagacg ggttatgcct ggtgggtgcg
    13861 ccgcatccgc catatgtgca gcatctatga tgtggtgcgc atcgaccact tccgcggttt
    13921 cgcgggatat tacgccatcc cctacggcga caagaccgcc gagcatggcc gctggcgcga
    13981 aggccccggc tatgcgctgt ttgccgccat caagaaaaag ctgggcagcc cccgcattat
    14041 cgcggaggat ctgggctttt tgaccgagga tgtcaacgcc ttgctgaaag agtgcggcta
    14101 ccccggcatg aaggtgttgg agttcgcctt tgacagccgc gacggcggcg actaccgccc
    14161 ccacagctac cagaacaact gcatcgccta cgtgggcacc cacgacaacg agccggtcaa
    14221 cggctggatg gagaccgccg cgcccgagga tgtggagcgc gccgtgcgct acctgaacct
    14281 gacgaagaaa gagggctacc actggggcat gatgcgcggc gtctggtcga gcgtggccga
    14341 cctggccgtt gtgcaggcgc aggatgtgct gggccttggg catgagtccc gcatgaacac
    14401 gccgtcgact ttgggcggca actggtgctg gcgcgccgag ccgcgggtgt tcaactcccg
    14461 cctggcccgc cgcctgcacg acctgatgga actgtacggc agactgcccg aataaacaaa
    14521 cgaccgccgc agcgcgtgaa aagcgccgcg gcgttttttt gccctcccgg ggggaggctt
    14581 tatgcagtgt ggccacaaat acaatatttt cccattgacc ttacaggccg gatacagtat
    14641 tctattagta gtataaattc taaagaggtg ttttcccgat gaaagtgtgt cagaaatgcc
    14701 atacgcccgc caggtatgat acgcagaaat tctgctcggt ctgcggcacg ccgttcccgg
    14761 atatgacgcc cgcggaaccc gccgccccgg cccggcagcc ggcccagccg gtgcagcccg
    14821 cgccccaggc accggtgcag ccgcctgtcc agcccccggt gcagccctac gcccaacagc
    14881 cgcagaaccc cttcggcgca gcggccccgc agcccgaaca gcccgagcag ccgaaaaaga
    14941 gcaagctgtc catcattatt gcggcagtcg tggtcgtagt cgttgtggct gccgctgcgg
    15001 cggtgttctt cctgcgcggc agcaaggaca atgcagccac cctgcctgcc gccgagccag
    15061 tcgccaccga agctgccgac gcggcggcgc cccagacgcc cgccccggcg gacagcgccg
    15121 cagcagccca gacacccgcg cccgctgtta cgccccagcc tgcccagacc ccggcccctg
    15181 cggcggcggt cgccaccgat acccaggtcg tgctgcgcaa cgcggcggcc aacggcacca
    15241 ttacggtgga cggtaaggcg gtggactttg cgtacgtggg caacgacgca gtcatccccc
    15301 gcagcagcct gtccgacgtc tgccaggtgc gcatcgtggc cgacaacggc aacggcggct
    15361 acaagaccgc cgccgtctgg tacaacaagg actacggcaa tgagctgacc ttcggcgcag
    15421 actacggcgc ttacaccgac tgcgacgcca ccggcctggc aaagcccggc gacaaggtcg
    15481 tggatgtgct gacctgggcg ttctaccgct ccttccagga ggctatcaac gagggaaatt
    15541 cctcgctgct gcgctattcc acggcgggca actcgacccg ctgcgctgcg gagattcgca
    15601 actacaccgg caacacctac gacctgggca acttccaggc agtcagcgac ccggcctcca
    15661 tcaaatacac cgatggccag gtcatctaca acggccactt tgtcgccaac tacacggaca
    15721 gcggcagcag ctacacagcg gaccatcacc gcacgctgcg ccttgtgtgg gaggacggca
    15781 tctggaaggt ggacgccttt tcgctgctgc ccgacggcgc gtacagcgcc accacctacg
    15841 cgaccctgcc gtaaccaccc catagaaggt acgcatgaaa aatacgaagt tgaaacttgc
    15901 ggcggcgtcg ctggccctgc tgctggcggg ctgcggcgca tcggccacgg tggaaccgca
    15961 gccgacgccc gccgcagcct tggcccccac ggcagccccg gcagtgaccg aagcccccgc
    16021 cgccacgccg gagccgatcc cgcccgagag ctatgaccag cgggacgaga cgttccgcgc
    16081 ccggcacgac ggcacggaag tgacggtaca cgctaccgtg accacgcctg ccgagggcgc
    16141 aggctggccg ctggtggtgc tctgccacgg ctttaccggc agccggggcg gcgacggcca
    16201 ttttggcccg ctggccgcac ggctggccca tgcaggcatt gccagcatcg ccatggactt
    16261 tcccggctgc ggcgagagcg aggaaccgtt cacggcctac acccttacga acatccgcgc
    16321 tgacatgacc gccgccattg cctatatggg ggtgacctac ggtgcggaca cgacccggct
    16381 ggggctggtg ggccacagca tgggcggccg cgccgtcagc ttgtacctgg acggccggga
    16441 ctaccctgtg accgccgctg ccctgtggag cccggcggac aataccgggc tggatgggct
    16501 ggaatttctg gcccatgatg ccgacgagcg ggcggccctg cgccagaccg ccgacgaaaa
    16561 cggcagcgtc gagctgcccc agtggggcgt ggccgccggt gcggccctgt ttgacgagat
    16621 ggccgccagc gacccctgcg gcgtgctggc cgagtatccg ggggcgctgc tggcggcttt
    16681 caccggcggc gacgccgagc tgttgagcga ggataccgtg gccctggtgc aaaacaccct
    16741 ggcaacgcgg gacctgccgt ttgtcgatat gacaaacgat ttccccgatg ccaaccacaa
    16801 ctaccaggcc gccgagggca gcgccgccag tgatgcggaa attgccgccc gcattgaaaa
    16861 cgccacggca gagtttttgg tttcggccct gcgctgaccg ttacagcgac gcgccctggc
    16921 tgagctgcgc ctggcagaag gcgaagaacc gctgcgccgc tgcgggctgc aaggccccgc
    16981 gcttgcggca gagataggct gtgcgccccg ggtcggggtc ggtcagcgga atgatcttta
    17041 aatccatgca gcgcagaaca gggtggtcgg gcacgatggc aatgccaaag cccgccgccg
    17101 ccataccggc tacgacggta tcttcctccg tctcgtaggc gattttcggc gttgcgccga
    17161 ttttttggaa cagggcatcg atgaggcggc gcaggccgcc gcgcttggaa aaatacacaa
    17221 acggataggg cagcgtctcc cgcagggtga cgctgttttt ttgtgccagc gggtggtttg
    17281 gcggcgtcac gacaacgaag ggcgaccggg caaaggcgac gttttcaaac accgtgtcgt
    17341 cgccggggtg gctgacgaaa gccatgtcga gccgttcctc ggccacggct tgcagcagat
    17401 cggcggtgaa gctgctctcg gtgtgtagct caaagcgggt atgttcgttg ccggatgtgg
    17461 caagaaattt ttgcatcaag tccggcacag tctgggccgc cagcttgcgg atgccgccca
    17521 gcagcaccag cccgccgccg cggcccatct cctgtaaatc ccgcgtcccg gcgtcatggc
    17581 gggggcgggc gcaggcacga tctacccggc ctgcctgcct acggccctca agcggttccc
    17641 cgacaaggcc ggttctatct ccagccttgt gcaggcgggc gcatcctgcg gccccttcat
    17701 catgtcgccc attgcccagt ggctcattga cacctacggt gcgcagatgg cctgccgcat
    17761 cctgggcagg ccgatgcgca cagcggcccc tgcaagggaa atggaaaatc cgccgacgac
    17821 atatttacac tgccctgaaa ttgtgctata ctatttctga ccgaccggtc ggtcgaaatg
    17881 aaagggaacg cagctatgcc gaaatatagt gtaaaaaaac catttacgat actggtggcg
    17941 gtcatactgg tgctggtgtt gggctttgtc agtctgaccg gcatccagac cgacctgctc
    18001 cccgcgatga acctgcccta cctgctggtc atgaccacct accccggtgc cagccctgag
    18061 caggtcgagg ccgacgtgac ccagccgctg gaaaacgcac tctcggtgct caacggcgtc
    18121 aagaatgtca cgagccaaag caacgagaat tacagcctga tcttcctgga ataccaggat
    18181 gacaccgaca tggacagcgc catggtcaag accagcacgg ccgtgaacca gctgggcgac
    18241 gcccttccgg atatggcgtc cactccgacg cttatggaaa tcagccctga catgatggcg
    18301 acccagtatg t
//
LOCUS       DBMDSQ010000009        17921 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_1018_length_17921_cov_2.563075, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000009 DBMDSQ010000000
VERSION     DBMDSQ010000009.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 17921)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 17921)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17921
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1018_length_17921_cov_2.563075"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..417)
                     /locus_tag="ACZPAX_00695"
     CDS             complement(<1..417)
                     /locus_tag="ACZPAX_00695"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015537079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR1112447.1"
                     /translation="MYKTVLFDLDGTLLNTIDDLADSANRVCAAHGWPQHTAAEYCYF
                     VGNGIPKLVERFSPADCRSPAQLAATLAEFDKVYGAHMHDKTAPYPGIPTLLARLKAA
                     GVRMAVFSNKADEFARAVVARYFDASLFEIVRGALPD"
     gene            589..>1227
                     /locus_tag="ACZPAX_00700"
     CDS             589..>1227
                     /locus_tag="ACZPAX_00700"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007048401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HIR1112448.1"
                     /translation="MELFLDLLRSVVYGVIEGITEWLPISSTGHMILAEQVLKFSLSA
                     EFMEMFRVVIQLGAIMAVVVLYFKKLWPFCGDNGCDSGLAKHIRWPVLRLWLKIIVAC
                     LPAAVLGLLLDDWMDAHFYNSVVVAVMLIVYGIAFILIERRPRVPATTKLSRITYKQA
                     IIIGAWQVLALIPGTSRSGATIIGGLLCGMSRACASQFTFFLAIPVMAGAPCL"
     assembly_gap    1228..1237
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1238..1472
                     /locus_tag="ACZPAX_00705"
     CDS             <1238..1472
                     /locus_tag="ACZPAX_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007048401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HIR1112449.1"
                     /translation="ASGLKLVKFLVGGGVFTVAEVGALLVGCIVAFVVSIMAIRFLMD
                     YVKKHTFTIFGWYRIALGILVLGIWALQSFVLA"
     gene            complement(1652..2650)
                     /locus_tag="ACZPAX_00710"
     CDS             complement(1652..2650)
                     /locus_tag="ACZPAX_00710"
                     /inference="COORDINATES: protein motif:HMM:TIGR01167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HIR1112450.1"
                     /translation="MAGGFCGEVTNDVLDLTNCFVYDTAISVTADNNAVLNSVGTFAG
                     GLYTNTHTTPPYYATVTTNNCYYGKVTLTPGSNPDTIKDTSTEKTAEEFANGTVANRL
                     GEAFTNGNGFPILGSSPADYTAVDAALAEANELDRTLYTDLSAVDTAVANVVRGYDRT
                     RQSDVDAMAQAILNALEHLTRKGADYTAVDAAITKANALNKDDYTNFTAVQAAIDAVD
                     RTKDITQQSEVDAMAADIQRALAALEKKPAPTAAPTAVPTAAPTQAPQESTAPAATQA
                     PTPAPTTAPTAAPTATPAPQAVSAIPATGDGANIALLWVMLVVSGGAALWIGRKKG"
     gene            complement(2897..3106)
                     /locus_tag="ACZPAX_00715"
                     /pseudo
     CDS             complement(2897..3106)
                     /locus_tag="ACZPAX_00715"
                     /inference="COORDINATES: protein motif:HMM:NF019217.6"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GLUG motif-containing protein"
     gene            3107..3313
                     /locus_tag="ACZPAX_00720"
     CDS             3107..3313
                     /locus_tag="ACZPAX_00720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1112451.1"
                     /translation="MIIAIIVTRKTGTSIVTVPDRVPSCALQINICSLLKVVPVEVAD
                     TAANIGIGITVGGFAAIDVIRQRL"
     gene            complement(3662..5113)
                     /locus_tag="ACZPAX_00725"
     CDS             complement(3662..5113)
                     /locus_tag="ACZPAX_00725"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR1112452.1"
                     /translation="MTDEQRREKQPLSMTRRYRRGLILTGVLCVLAMLAAATVLFQRS
                     YTDRVDDYLAQLGGSYAAAYKAQPTHDAKTLVSLLPKPGVDAPRFTLIGGDGTVLFDS
                     KTSALVYDAANTVYAVMDSHLPSHADRPEFQQAMADGTASVTRESETVRRETHYYAVR
                     IDTPDGAQVLRVGEDIANIWGLSTDTLPLLCAAMVLILAAATLFSWWLTRRMVQPINH
                     LAEHLDTIEADVPYQELIPLARTIQTDRKLREDNETMRREFTANVSHELKTPLTSISG
                     YAELIETGMAKPADVPTFAARIHKEAQRMIALVSDILQLSELDSTQASHSRETVTEFA
                     PVDLAALVKETAQNMTVNARRAYVTLQYDVRPATVRGSRDQLSELAQNLCDNAIRYNR
                     PGGHVELRCGVGNDGCPYFEVEDNGIGIPQDSQTRVFERFYRVDKSRSKATGGTGLGL
                     AIVKHIALLHDAKIDLQSQVGTGTTIRVTFPKG"
     gene            complement(5106..5798)
                     /locus_tag="ACZPAX_00730"
     CDS             complement(5106..5798)
                     /locus_tag="ACZPAX_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007048666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR1112453.1"
                     /translation="MIYIVEDDSSIRELEQYALQSNGYDARGCEDAASFWAALRDTRP
                     ELVILDVMLPDEDGYQILTKLRANTATASIPVIMVTAKTSEIDVVKGLDHGADDYLCK
                     PFGIMEFISRVKAVLRRAGAAAPAPAASTLHFGEIVLDDMARTVRVGGAPVELTFKEY
                     ALLHLFLEHPEEVLARERIMKDVWDTDDLLESRTIDMHVRTLRQKLGAAGEAIRTVRK
                     VGYKLSTEGSDD"
     gene            complement(5946..7739)
                     /locus_tag="ACZPAX_00735"
     CDS             complement(5946..7739)
                     /locus_tag="ACZPAX_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015564098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HIR1112454.1"
                     /translation="MTIFNVFTLMGGIAMFLYGMDLMGKALEQTAGSKLQGILSTMTS
                     SPIRGLLLGMAVTAVIQSSGATTVMAVGFVNSGLMELHQAISVIMGANVGTTVTGWLL
                     SLSGLEGDSFITQMMNPNAWSPILGFIGIWLYMIGKDRKRGVGKILLGFSILMAGMNT
                     MSHAMSPLADEPWFMDLFLSFKNPILGVIAGAVLTAVLQSSSASVGILQALTSTGAVT
                     YSAAVPIIMGQNIGTTVTALISSAGANKNAKRTAFVHLYFNVIGTILFLCGFYGLNAL
                     IQFSFFNETANTFGIAIVHTVFNVVTTAILLPFNRLLEKLAILTVPDSPADKKQENTL
                     LDERLLTTPSVAVGRAMLTGSDMAEICRTALLQAMSTTRVWNDAIADEVLRKEDAVDH
                     YEDVLGTYLVKLSAKHLSGEDNRTINTLLHTIGDFERVSDHAVNIIKTAEEIRTKNIK
                     FSEEALNDLSVLEAAVQDIVNRTVDAFQKGDTYAAGKIEPLEQVVDGLVREVKTRHIA
                     RLQAGVCTIEYGFVLDDLLTNYERIADHCSNIAVAMIEVAADKFDTHEYLANVKHGGS
                     VKFEQRYEKYRGRYTFPPEAYSEPAENQAEG"
     gene            8730..9695
                     /locus_tag="ACZPAX_00740"
     CDS             8730..9695
                     /locus_tag="ACZPAX_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007048670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HIR1112455.1"
                     /translation="MKRTSAIAAFTAAALALSLAACGSSDSAADTAAESTAASTEAVS
                     SEAASEAAPAESTGDFDTDQDITVISREDGSGTRGAFIELTGVEEKNADGKKVDNTTE
                     AAAIQSSTNGVMTAVANDETAIGYISLGSLNDTVKAVTVGGVAASAETVKDGSYVLAR
                     PFNIVTNGDATDPVAVDFIAYCLSKDGQALATDKGFIGEDGADFTSAQPAGKITVGGS
                     TSVSPLMEKLIEAYKTVNPNAELELLTTDSTTGVSGALDGTYTIGMASRELKDSETEA
                     GAQATVLALDGIAVVVNPANTTEDLTVDQIKGIYTGELTTWADVQ"
     gene            9824..10717
                     /gene="pstC"
                     /locus_tag="ACZPAX_00745"
     CDS             9824..10717
                     /gene="pstC"
                     /locus_tag="ACZPAX_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007048671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HIR1112456.1"
                     /translation="MTSKLKEGVMQVVFTLCACVSILAVALICIFLFANGVPTLFKIG
                     PLKFLLGTTWKPGNDIFGILPMILGSIYVTAGAILVGVPIGLLTAIYMSRFASPRINK
                     IMLPAVQLLAGIPSVVYGFFGMIVLVPAVQAMVKTPFFKTVLHVKSGKGMSLFTAAVL
                     LGIMILPTIITVSKTSLDAVPNSYYEGSLALGATHERSVFCAVLPAARSGVLSAVILG
                     IGRAIGETMAVVMVAGNQTWMPKGLFQGLRTMTSNIVIEMGYAADLHREALIATGVVL
                     FVFILLINLCFSLVKGGSDHA"
     gene            10773..11618
                     /gene="pstA"
                     /locus_tag="ACZPAX_00750"
     CDS             10773..11618
                     /gene="pstA"
                     /locus_tag="ACZPAX_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007048672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HIR1112457.1"
                     /translation="MPGGGQNRRAARAQAAALRWLVRIAAFLTAAVLVFLIGYILVMG
                     IPNLKPELFAWEYNSENVSLMPALINTVLMTAFALLIATPLGIFAAIWLVEYARRGSK
                     LVKVVRVTTETLQGIPSIVYGLFGMLFFVTQLHWGYSLIAGAFTLAIMVLPVIMRTTE
                     EALIAVPDSYREGSFGLGAGKLRTVFTIVLPSAMPGILSGVILATGRIVGETAALIYT
                     ASSVAKIPTSVFSSTRTLAVHMYLLSNEGLYIGQTYGTAVVLLVLVLVINCLSGFLAG
                     KLAKK"
     gene            11813..12565
                     /gene="pstB"
                     /locus_tag="ACZPAX_00755"
     CDS             11813..12565
                     /gene="pstB"
                     /locus_tag="ACZPAX_00755"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015557761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HIR1112458.1"
                     /translation="MNKFEVRGMDLYYGSFHALKNINIDIPEKEITAFIGPSGCGKST
                     FLKSLNRMNDLVEGCRISGDIKLDGVDIYKDKMDVNVLRKRVGMVFQKPNPFPMSIYD
                     NIAYGPRTHGIRNKAQLDEIVEKSLRGAAIWDEVKDRLNKSALGMSGGQQQRLCIARA
                     LAVEPEVLLMDEPTSALDPISTSKVEELATELKENYTIVMVTHNMQQAARISDKTAFF
                     LLGELVEMGPTERVFATPVDKRTEDYISGRFG"
     gene            12577..13251
                     /gene="phoU"
                     /locus_tag="ACZPAX_00760"
     CDS             12577..13251
                     /gene="phoU"
                     /locus_tag="ACZPAX_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007048674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HIR1112459.1"
                     /translation="MGTSVRKMYDAELLQLDTMLARMGHAAGGAIESAMTALRTGDTE
                     LARSVIARDSEIDSAEHEIEHRCLTLFLRQQPVAGDLRKVSTALKMVTDIERIADQAS
                     DIAEITLHLHPEGARTVGVLDDLYAMGDIALRMVKDSIAAYVQVDLSTAAQVIARDDE
                     CDAMFSRISQEIAAYIAAHPDDASTALDLFMIDKYLERLGDHAVNIAEWVEFLKTGVH
                     KSRKIV"
     gene            complement(13561..15012)
                     /locus_tag="ACZPAX_00765"
     CDS             complement(13561..15012)
                     /locus_tag="ACZPAX_00765"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005931089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HIR1112460.1"
                     /translation="MTSKREKLQYAYVFLTDFVTTGISVVLAWLITDNVFNKLQDYAT
                     FDWIQALCLLLLAFVLTFLCFDQDQNIVTRSKGDEIILALKFNVVLGVVNAACLVLAK
                     ATLLDSRYFAVMVPIVNVVLMPITHGLLKKFLLKLQHSRGSESLVGFVTTCDRAEKLI
                     AEIQGDWSRRIIGVALLEATPETVGTRVAGVEVKATFDDFMAWLRREALDEVYVDVPM
                     DSGESFIPYLEEMESMGLTVHFRLPLLDHIEARCCDETSSARLSRTLGRCAGGNIVTM
                     GTVEMKLRDRVAKRAMDIAGAIVGCAISIPIIAVVAIPLKIESPGPLFFKQKRVGLNG
                     RYFYIHKLRSMYMDAEERKKELMAKNEMNGLMFKMEDDPRVTKVGKFIRKTSIDELPQ
                     FFDVLRGDMSLVGTRPPTVDEYKQYESHHKRRLSMKPGITGLWQVSGRSNIEDFEEVV
                     KLDVTYIDNWSLWNDVKILFKTVYVVFAGRGAK"
     gene            complement(15256..17172)
                     /locus_tag="ACZPAX_00770"
     CDS             complement(15256..17172)
                     /locus_tag="ACZPAX_00770"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015565919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HIR1112461.1"
                     /translation="MTREQVEKEMAQYRTIFTVVRLLEAAQVGSDKTLSDYCKCYDFW
                     QKHTQCQNCISAQVLRDHTQRTKLEYMGSQVFQVTAVYREVDGQPCVMELIQQLDSET
                     LIDPENSNRLMDSVTGYHFKLYHDALTGGYNRRYFEDVLKDKTDPAGVAVLDLDDFKL
                     YNDTYGHHAGDLALRTAMEVVRANIRKSDSLIRYGGDEFLLVLPGIERGAFNAKLQRI
                     CKKIHAATVPGYSCLQLSVSIGGVMSQPDETVEHAVGRADKLMYQAKNHKNTVVTEDT
                     AAPRASTAAGRENQTRQNILIVDDSEMNRAILSEILGSDYNILEAANGKECLAMLEQY
                     DTGIALILLDIVMPVMDGFEVLAAMNRNHWIEDIPVIMISSEDSDPVVRHAYEMGVSD
                     YVSRPFDASIVYRRVFNTIKLYAKQRRLISLVTSQTREKEKNTQMMVSILSEVVEFRN
                     GESGLHVQHIGTLTQRLLEHLTQKTDKYDLPPEKQELIVLASALHDIGKIAIDDKILN
                     KPGRLTQEEFNIMKTHTVIGANMLDKLGIYRSEALVRTAYEICRWHHERWDGRGYPDG
                     LSGDRIPISAQVVSMADVYDALVSKRVYKAAYAPDTAVQMILHGDCGTFNPLLLECLV
                     EIQDVLKADVTEAE"
     gene            17320..>17921
                     /locus_tag="ACZPAX_00775"
     CDS             17320..>17921
                     /locus_tag="ACZPAX_00775"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014675.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 10 protein"
                     /protein_id="HIR1112462.1"
                     /translation="MRRVVFYLFIIAVFLAAPLAALPRRGETAPAATPAPTATAEPTA
                     AAPYRAVWVSYLEWQQVDFSATETFSDDIAVILDNIRNLGATVVLAQVRPFGDALYPS
                     SYYPFSHLCTGQQGRDPGYDPLALLVQAAHDRGLALEAWVNPYRIQAGLTPALCDASP
                     AKLHPDWVKYTDTGAYLDPANTAVRQDIADAVGELCANYDV"
BASE COUNT         3452 a         5410 c         5366 g         3683 t
ORIGIN      
        1 gtcgggcagg gcaccgcgca cgatttcaaa caggctggcg tcaaaatacc gggccaccac
       61 cgcgcgggca aactcgtccg ccttgttgga gaacacggcc atccgcaccc cggcggcttt
      121 cagccgggcc agcagtgtcg gtatgccggg gtagggcgcc gtcttgtcgt gcatatgcgc
      181 gccgtatact ttgtcaaact cggccagcgt ggcggccagc tgggccgggc tgcggcagtc
      241 ggcggggctg aaccgctcca ccagcttggg gatgccgttg cccacaaagt agcagtactc
      301 ggccgccgtg tgctggggcc agccgtgggc cgcgcagacc cggttggccg agtcggccag
      361 atcgtcgatg gtattcaaaa gggtgccgtc caaatcaaac agtacggttt tatacatttt
      421 ccaaaacctc tttgcacaag aaatcactct tattatagtt gcatttgacg aaaacagcaa
      481 cccaccgcgc ccgtttaccg taaaaaagat gtaaaatctc aaaaaaatgt tcccattttc
      541 ttgttttatg gtatgatagt aggtagcaaa taaggaggct tcccacttat ggaattgttt
      601 ttggacctgc tgcgctcggt cgtctacggc gtgatcgagg gcattaccga atggctgccc
      661 atctccagca ccggccacat gattttggcc gagcaggtgc tcaagttcag cctgagcgcg
      721 gaatttatgg agatgttccg cgtcgtcatc cagctgggcg cgatcatggc ggtcgtggtg
      781 ctgtatttca aaaaactgtg gccgttctgc ggcgacaacg gctgtgattc cggcctggca
      841 aagcacattc gctggcctgt gctgcgcctg tggctgaaaa tcattgttgc ctgcctgccc
      901 gctgccgtgc tgggcctgct gctggacgac tggatggacg cgcattttta caacagcgtc
      961 gtcgtggcgg tcatgctcat cgtctacggc atcgcgttta tcctcatcga gcgccgcccc
     1021 cgcgtgcccg ccaccaccaa attgagccgc atcacctaca aacaggcgat catcatcggc
     1081 gcatggcagg tgctggcgct catccccggc acgtcccgca gcggcgcgac catcattggc
     1141 ggcctgctct gcggcatgag ccgcgcctgc gcgtcgcagt tcaccttctt cctggcaatc
     1201 ccggtcatgg cgggtgcccc gtgtctcnnn nnnnnnntgc gtcgggcctc aagctcgtca
     1261 agttcctggt gggcggcggc gtgttcaccg ttgccgaggt cggtgccctg ctggtgggct
     1321 gcatcgtggc gtttgtcgtg tccatcatgg caatccgctt cctgatggat tacgtcaaga
     1381 agcacacctt cacgatcttc ggctggtacc gcatcgccct gggcatcctg gttctgggca
     1441 tctgggcgct gcagagcttc gtgctggcgt aaaatacaca atagaaaatc cgctgcttcc
     1501 ccatgccggg gaagcagcgg attttttgtg gcggttgcgc ggtaggggcc gggcatgccc
     1561 ggcccgcggc tttcccgcag gcgcgatttt acctttctgt tgcgggccgg acatgtccgg
     1621 cccctacaac tttatggatt tttcgggaac gttacccttt cttgcgcccg atccacaggg
     1681 ccgcgccgcc gctcactacc agcatcaccc acagcagggc aatattcgcg ccatccccgg
     1741 tagcgggaat cgcgcttacc gcctgcgggg caggggtggc tgtgggcgca gcggtgggag
     1801 ccgtagtcgg tgcaggcgtg ggggcctggg tcgcagccgg ggcggtgctc tcctgcgggg
     1861 cctgggtggg ggccgcggta ggcaccgctg tgggggctgc cgtcggcgcg ggcttcttct
     1921 ccaacgcggc aagggcacgc tggatgtcgg cggccatggc gtccacttcg ctttgctggg
     1981 taatatcctt cgtgcggtca acggcatcaa tggccgcctg caccgccgta aagttggtgt
     2041 aatcgtcctt gttcagggcg ttggccttgg tgatggcggc atccacggcg gtgtagtccg
     2101 cacctttccg cgtcagatgc tccaacgcgt tcaggatagc ctgcgccatg gcgtctacgt
     2161 cggactggcg ggtgcggtca tagccgcgca cgacattggc aacggcggta tccacggcag
     2221 aaaggtcggt gtacagcgtg cggtcaagct cgttggcctc ggcaagagcg gcgtcgactg
     2281 cggtataatc ggcggggctg gaacccagaa tggggaaacc gttgccgttc gtgaaagcct
     2341 cgcccagccg gtttgcaact gtgccgttgg cgaattcttc cgcggtcttt tcggtggaag
     2401 tatcttttat ggtgtcgggg ttgctgccgg gggtaagagt aactttgccg tagtagcagt
     2461 tattagtagt tacggttgcg taatacggcg gggtggtatg tgtattggta tataggccgc
     2521 cagcaaaagt accaacactg ttaaggactg cattattgtc ggcagtaacg gaaatagcag
     2581 tgtcgtaaac gaagcagttt gtcaggtcga gaacatcatt agtgacctcg ccgcagaagc
     2641 cgccagccat actgaaccca ttggagctag gagctgagac attgacagtg gagccagcta
     2701 cgtaacaatc tacaaatttg ctggcgtcaa caccctcgta atcatcgtcc tgctcgccaa
     2761 caaagccgcc gctgtatgag gttgatgtaa cgatattgtt gttagcggca cattttgaaa
     2821 aagtgctgcc aatagaatat ccaacaaggc cgcctgcaca gttgttattg ttataagaag
     2881 tattgctgct tatcttggaa ttggagacgg agcagtttgt aatttcggaa gcataaacag
     2941 caccgacaag accgccagcg aaagcgtagt tgccattacc tgccgcagaa aacgttacat
     3001 tttctatatg cagattgctg acagaaccat tccacacgaa accaaaaata ccagcggagg
     3061 tataaccgtt gtctgttagg cctgaggaaa tggcattgca aatcacatga ttatcgccat
     3121 catagtgacc agaaaaacag gcaccagcat tgtcactgta cccgataggg ttccaagctg
     3181 cgccctgcaa atcaatatct gcagtctgct taaagtagta cccgtggaag ttgccgatac
     3241 cgccgcaaac atcggcatcg gcatcacagt cggcggcttt gccgctattg atgtaatccg
     3301 ccagcgcctt taaatcggcg gcggtactga taagccaggg gtcgtcctgc gtgccgctgc
     3361 ccccgctcaa aaattcattg gggggggggt gacggcgaaa gccgagatgg gcagcgcggt
     3421 gaagcagagc accagcgcca gcagcagact taggaacttg ctgttgtgtt tcatggggtc
     3481 gcgtcctttc ctgtaataaa atactatata aagtcatttt aatacgcaca tgggcaaaat
     3541 gcaagatggg tagggaaatt ttggggaatg agaaagaaaa aaccggaacg gctgggggcc
     3601 gtcccggtgg tgggggtgtt aaggggatag gggcttttgc aatccagcct ttgttgtagc
     3661 cttacccctt cgggaacgtc acccgtatcg tcgtgccggt cccgacctgg ctttgcaggt
     3721 ctatcttcgc atcatgcagc agggcaatgt gcttgacgat cgccaatccc aggcccgtgc
     3781 cgccggtggc tttggaacgg ctcttgtcca cgcggtagaa ccgctcaaac acgcgggtct
     3841 ggctgtcctg cgggatgccg atgccgttgt cctcgacctc aaaatagggg cagccgtcat
     3901 tgcctacgcc gcagcgcagc tccacatggc cgcccgggcg gttgtagcgg atggcattgt
     3961 cgcacaaatt ctgcgcaagc tcgctcaact ggtcgcggct gccgcgcacg gtggcgggcc
     4021 tgacgtcgta ttgcagcgtt acataggccc ggcgggcgtt caccgtcatg ttctgggccg
     4081 tctccttgac cagcgcggcc agatccaccg gggcaaactc ggtcacggtc tcacggctgt
     4141 ggctggcctg ggtgctgtcc agctcgctca gctgcaaaat atcgctgacc agcgcgatca
     4201 tccgctgtgc ttccttgtgg atgcgcgcgg cgaacgtcgg cacgtcggcg ggctttgcca
     4261 tgccggtttc gatcagctcg gcatagccgg aaatgctcgt cagcggggtt ttcagctcat
     4321 ggctgacatt ggcggtaaac tcccggcgca tcgtctcgtt gtcctcccgc agcttgcggt
     4381 cggtctggat ggtccgtgcc agggggataa gctcctgata gggcacatcg gcctcgatgg
     4441 tgtccaggtg ctcggccagg tggttgatag gctgcaccat gcggcgggtc agccaccagc
     4501 tgaacagcgt tgcagcggcc aaaatcagaa ccatggccgc gcagagcagc ggcagggtgt
     4561 cggtgctcag gccccagatg ttggcaatat cctcgcccac gcgcagcacc tgcgcaccgt
     4621 cgggcgtgtc gatgcgcacg gcgtagtagt gggtctcccg gcgcacggtc tcactctcac
     4681 gggtgacgct ggcggtgccg tcggccatgg cctgctggaa ctccggccgg tcggcgtggc
     4741 tgggcaggtg ggagtccatg acggcgtaca cggtgtttgc ggcatcgtag acaagggccg
     4801 aggtcttgct gtcaaacagc acggtgccgt cgccgccgat aagcgtaaag cgcggcgcat
     4861 caacgcccgg tttcggcagc aggctgacca gcgttttggc gtcgtgggtg ggttgggcct
     4921 tgtaggcagc ggcgtagctg ccgcccagct gggccaggta gtcgtccacg cggtcggtgt
     4981 agctgcgctg gaacagaacg gtggccgccg ccagcatggc cagtacgcag agcacgccgg
     5041 tcaggatcag gccgcgccga tagcggcggg tcatgctcag gggctgtttt tcgcggcgct
     5101 gctcgtcagt catcactgcc ctccgtgctc agcttgtagc ccaccttgcg cacggtgcgg
     5161 atagcctcgc ccgccgcgcc cagcttttgg cgcagcgtgc gcacgtgcat atcgatggtg
     5221 cgggattcca gcagatcatc ggtgtcccag acgtccttca tgatgcgttc acgggccagg
     5281 acttcctccg ggtgctccaa aaacagatgc agcagcgcat attctttaaa ggtcagctct
     5341 accggcgcac cgccgacgcg gaccgtgcgg gccatgtcgt ccagtacgat ttcgccaaaa
     5401 tgcagcgtcg atgcagcggg cgcgggggcg gccgctcccg cgcggcgcag cacagccttg
     5461 acgcgggaga tgaactccat gatgccgaag ggcttgcaca ggtagtcgtc cgcgccgtgg
     5521 tccagcccct tgacgacgtc gatctcgctg gtcttggccg tgaccataat gaccgggatg
     5581 gacgccgtgg cggtgttggc gcggagcttg gtcaaaatct ggtagccgtc ctcgtcaggc
     5641 agcatgacgt ccagaatgac cagctcgggc cgggtatcgc gcagtgcagc ccaaaagctg
     5701 gccgcgtcct cgcagccgcg ggcgtcatag ccgttggact gcagcgcgta ttgctccagc
     5761 tcacggatgc tggaatcgtc ctcgacgata tagatcatag taaatactcc ttactataaa
     5821 cgtagggacc gggcatgccc ggcccgcggc tttgcgtggc cgtccgataa cgggcggctg
     5881 cttttctgtt gcgggcgggg cataccccgc ccctacaaaa aggcttcccc ctcggggaaa
     5941 ggctttcagc cttcggcctg attctccgca ggctcggaat atgcctccgg cgggaaggtg
     6001 tagcggccac ggtatttctc gtagcgctgc tcaaatttta cgctgccgcc gtgcttgacg
     6061 ttggcaagat actcgtgggt gtcgaacttg tccgccgcaa cctcgatcat cgcgaccgca
     6121 atgttcgagc agtggtcggc gatgcgctcg tagttcgtca gcagatcatc cagcacaaag
     6181 ccgtactcga tggtgcagac accggcctgc aaacgggcaa tgtggcgggt cttgacctcg
     6241 cgcaccaggc cgtccacgac ctgctccagc ggctcgatct tgccggcagc gtaggtgtcg
     6301 cccttctgga atgcgtctac cgtgcggttg acgatatcct gcacggcggc ttcaagaacg
     6361 gacaaatcgt tgagggcttc ctcgctgaat ttgatgtttt tcgtgcggat ctcctcggcc
     6421 gttttgatga tgttcacggc gtggtcggac acacgctcaa aatcgccgat ggtgtgcagc
     6481 agcgtgttga tggtgcggtt atcctcgccc gaaagatgct tggcgctcag cttgaccagg
     6541 taggtgccca gcacgtcctc ataatggtca acagcgtcct ctttgcgcag gacttcatcc
     6601 gcaatggcgt cgttccagac gcgggtggtg gacatggcct gcaaaagcgc ggtgcggcag
     6661 atttccgcca tatcgctgcc ggtcagcatg gcgcggccaa cagccacaga gggggtggtg
     6721 agcagacgct cgtccagcag ggtgttctcc tgctttttgt cggcggggga gtcgggcacg
     6781 gtcaggatgg ccagcttttc cagcaggcgg ttgaagggca gcaaaatagc ggtcgttacc
     6841 acgttgaaaa ccgtgtgaac gatggcgatg ccgaaggtgt tggcggtctc gttaaagaaa
     6901 gagaattgga tgagcgcgtt cagcccatag aacccgcaca ggaagaggat cgtgccgata
     6961 acgttaaaat agagatggac gaaggcggtg cgcttggcgt ttttgttggc accggcggag
     7021 gaaatcagcg ctgtgacggt ggtgccgatg ttctggccca tgatgatggg cacggcggcg
     7081 gagtaggtga cggcgccggt gctggtcaac gcctgcaaga taccgacgga ggccgacgag
     7141 gactgcaaaa cagccgtcag cactgcgccc gcgataacgc cgaggatggg atttttgaag
     7201 ctgaggaaca ggtccatgaa ccacggctcg tcggccagcg gggacatggc atgggacatc
     7261 gtgttcatgc cggccatcaa aatagagaag cccagcagga ttttaccgac gccgcgcttg
     7321 cggtccttgc cgatcatata cagccagatg ccgataaagc ccagaatcgg gctccaggca
     7381 ttggggttca tcatctgggt gatgaagctg tcaccctcga ggcccgacag cgacagcagc
     7441 cagccggtga cggtggtgcc gacattcgcg cccataatga cgctgatggc ctggtgcagt
     7501 tccatcaaac cggagttgac gaaaccaacg gccataacgg tggtggcacc gctggactgg
     7561 atcacggcgg tgacggccat gccgagcagc aggccgcgga tggggctgct ggtcatggtg
     7621 gacaggatac cctgtaattt gctgccggcg gtctgctcca gcgctttccc catcaggtcc
     7681 atgccataca ggaacatggc aatgccgccc atcagggtga acacattaaa aatggtcatc
     7741 cggtgttaca catccattct cttattttgt ttacactata ccgccttcta ttataatgta
     7801 tgcgcgcagg ctacaccacc acaggaaagt aaatgttgtg taaaatttgt gtaaagccgc
     7861 agggagcggc agtctcccgg aaggttgttc tttcttttgg cagcaaaaga aagaaccaaa
     7921 gaaaactgcc gctgtttcga cgcagcggac ccgcgaccta ggggctgcac gcccctagga
     7981 accccaaagc ggaggtcgga agtttcggcg gctaaacgga cgcagtatta ctgtccgttc
     8041 ttaacgctgc cgaaccttcc tccactttaa attcgggtgt tgcggggttg cgagcgaggg
     8101 gccacagttg agcggagcga aactggggta gtgagcaatg cttgcccctg cggggcaagc
     8161 aaccgcacga gcgggccagc tttgctggct ggcggcgctc gttgcgcgcc gccaagcgcc
     8221 ccgaaccccc gcagttgtaa acttccgcct atttgtaggg gccgcatata tgcggcccgc
     8281 caacctgccg ccacatcccg tttccccatc accaccctgt aggggccggg catgcccggc
     8341 ccgcaactta ccgctaaatc ccttctacgg tttaatccgt aggggaggga ttcatccctc
     8401 ccgggcacct tcccgccgcc gcaaacggcc acggcggcca gagggctggc cgccctacag
     8461 ccccacttaa aaatccgtgc gtagggcggg gtgccctcac cccgccgcct ctcttcccat
     8521 cccgcacccc ccactgtttt tgcatattct ttacaaactt taccgttccc acacaaaatc
     8581 ggggacatct ttgcaaaacc tttaccgcga ttgtaccgta ccatggtcga tacccgcaaa
     8641 aaaaggcggt acaatagggt cgttccgatg agaacacacc caaaacacta taccaaaaac
     8701 aatattgggg aaaagggaga atactttaca tgaaacgtac ttctgcaatc gctgctttta
     8761 ccgctgccgc tctggcactg agccttgccg cctgcggttc ctctgattcc gccgctgaca
     8821 ccgccgccga gagcacggcg gcttccaccg aggctgtctc ctcggaggct gcttccgagg
     8881 ctgcccctgc cgagagcacc ggcgacttcg acaccgacca ggatatcacc gtcatctccc
     8941 gagaggacgg ctccggcacc cgcggcgcct tcattgagct gaccggcgtt gaggagaaga
     9001 acgccgacgg caagaaggtt gacaacacca ccgaggcggc cgccatccag tccagcacca
     9061 acggcgtcat gaccgctgtt gccaacgatg agaccgccat cggctacatc agccttggct
     9121 ctctgaacga caccgtcaag gccgtcacgg tcggcggtgt ggccgcctcc gccgagaccg
     9181 tcaaggacgg cagctatgtg ctggcccgtc cgttcaacat cgtcaccaac ggcgatgcca
     9241 ccgaccccgt ggccgtggac ttcatcgcct actgcctgag caaggacggt caggcgctcg
     9301 ccaccgataa gggctttatc ggcgaggacg gcgcggactt cacctccgcc cagcctgccg
     9361 gcaagatcac cgtcggcggc tccacctccg tctctccgct gatggaaaag ctgatcgagg
     9421 cctacaagac cgtcaacccc aacgccgagc ttgagctgct caccaccgac tccaccaccg
     9481 gtgtttccgg cgcgctggac ggcacctaca ccatcggcat ggccagccgt gagctgaagg
     9541 actccgagac cgaggccggt gcccaggcta ccgttctggc actggacggc atcgccgtcg
     9601 tcgtcaaccc tgccaacacc accgaggatc tgaccgttga ccagatcaag ggcatctaca
     9661 ccggcgaact gaccacctgg gccgatgtgc agtaaagtat agcagcaccg taggggcggg
     9721 gcatgccccg cccgcagcgt acccatgacc gcgcggttat gggtaggccg cgggccggac
     9781 atgtccggcc cctacatggc atataaccaa aggagtatag gaaatgacct ccaagttaaa
     9841 agaaggcgtc atgcaggtgg tgttcacact ctgcgcctgt gtatctattc tggcagtggc
     9901 gctcatctgc atcttcctgt ttgcgaacgg cgtgcctact ttgttcaaaa tcggcccgct
     9961 caagttcctg ctgggcacca cttggaagcc cggcaacgat atcttcggca tcctgccgat
    10021 gatcctgggc agcatctacg tcacggcggg ggctatcctc gtcggcgtgc ccatcggcct
    10081 gctgaccgcc atttatatgt ccaggtttgc ctcgccccgc atcaacaaaa tcatgctccc
    10141 ggcggtgcag ctgctggcgg gtatcccgtc ggtcgtgtac ggcttcttcg gtatgatcgt
    10201 gctggtgccc gccgtgcagg ccatggtcaa gacgcccttc ttcaagacgg tgctccacgt
    10261 caaaagcggc aagggcatga gccttttcac cgccgctgtg ctgctgggca tcatgatcct
    10321 gcccaccatc atcacggtca gcaagacgag cctggacgcc gttcccaaca gctactatga
    10381 gggcagcctg gccctgggcg cgacccatga gcgcagcgtt ttctgcgcgg tgctgcccgc
    10441 cgccaggtcc ggcgtgctgt cggcggtcat tctcggcatc ggccgtgcca tcggcgagac
    10501 gatggcggtc gtcatggtcg cgggcaacca gacatggatg cccaagggcc tgttccaggg
    10561 cctgcgcacg atgacctcca acatcgtcat cgagatgggc tacgccgccg acctccaccg
    10621 cgaggcgctg atcgccaccg gcgttgtgct gttcgtcttt atcctgctca tcaacctctg
    10681 cttcagcctt gtgaaagggg gcagcgacca tgcctgagat tgccgcaacg caaacggccg
    10741 ccgatatgac ctacgcaaaa ccccatgagt ttatgcccgg cggcgggcag aaccgccgcg
    10801 ctgcccgcgc gcaggctgcc gcgctgcggt ggctggtgcg gatcgccgcc ttcctgacgg
    10861 ccgccgtgct ggtgttcctg atcggctaca tcctcgtcat gggcatcccc aacctcaagc
    10921 ccgagctttt cgcatgggag tataactccg agaatgtctc gctgatgccc gcgcttatca
    10981 ataccgtgct gatgaccgcc ttcgcactgc ttatcgccac gccgctgggc atctttgccg
    11041 ccatctggct ggtcgagtac gcccgccgcg gcagcaagtt ggtcaaggtc gtgcgcgtca
    11101 ccaccgagac cttgcagggc atcccgtcca tcgtctatgg cctgttcggt atgctgttct
    11161 tcgtcaccca gctgcactgg ggctactcgc tgatcgccgg tgcgtttacg ctggccatca
    11221 tggtattgcc cgtcatcatg cgcacgaccg aggaagcgct catcgccgtg cccgactcct
    11281 accgcgaggg cagcttcggc ctcggcgcgg gcaagctgcg cacggtgttc accatcgtgc
    11341 tgcccagcgc catgcccggt attttgtccg gtgtcattct ggcgacgggc cgcatcgtgg
    11401 gcgagactgc cgcgctgatc tacactgcca gctcggtcgc caagatcccc accagcgtat
    11461 tcagctcgac ccgcacgctg gccgtacata tgtacctgct ctccaacgag ggtctctaca
    11521 tcggccagac ctacggcacc gccgttgtgc tgctggtgct ggtgctggtc atcaactgcc
    11581 tgtccggctt tctggccggc aagttggcga agaagtaaag ccgcctgcgg ggcgtcgagg
    11641 acgccgcccc ctgcatacgc agcagcttgt aggggccggg catgcccggc cctctgcaga
    11701 cccattacca ctctgcttcc gtaaggctga gggccggaca tatccggccc ctacggctcg
    11761 gcaaaggcat atatgcggtc tataaacaaa gatacagcaa agaggtcatt tcatgaacaa
    11821 atttgaagtc cgcggcatgg atttgtacta tggcagcttc catgcgctga aaaatatcaa
    11881 catcgacatc ccggaaaagg agatcactgc cttcatcggg ccttccggct gcggtaaatc
    11941 cacgtttttg aagagcttga accggatgaa cgacctggtc gagggctgcc gcatctccgg
    12001 cgatatcaaa ctggacggcg tggacatcta taaggacaag atggacgtca acgtcctgcg
    12061 caagcgcgtc ggcatggtgt tccagaagcc gaacccgttc ccgatgtcga tctatgacaa
    12121 cattgcctac ggcccgcgca cccacggcat ccgcaataag gcgcagctgg acgagatcgt
    12181 cgaaaaatcc ctgcgcggcg cggccatctg ggacgaggtc aaggaccgcc tgaacaagag
    12241 cgccctcggt atgtcgggcg gccagcagca gcgcctgtgc attgcccgcg ccctggcggt
    12301 cgagccggag gtcctgctca tggacgaacc caccagcgcc ctggacccca tctcgacctc
    12361 gaaagtcgaa gaactggcca ccgagctgaa agagaactac accatcgtca tggtcaccca
    12421 caatatgcag caggccgcgc gtatcagcga taagacggcg tttttcctgc tgggcgaact
    12481 ggtggaaatg ggcccgaccg agcgcgtctt tgccacgccg gtggacaaac gcaccgagga
    12541 ttacatctcg ggccgtttcg gttgaggagg tttacaatgg gtacaagtgt acgcaaaatg
    12601 tacgatgcgg aattgctgca gctggacacg atgctggccc gcatgggcca cgcggcgggc
    12661 ggcgccatcg agagcgccat gaccgccctg cgcaccggcg acaccgagct ggcccgcagc
    12721 gtgatcgccc gggacagcga gatcgactcc gccgagcatg agatcgagca ccgctgctta
    12781 acgcttttcc tgcggcagca gccggtggcg ggcgacctgc gcaaggtgtc cacggcctta
    12841 aaaatggtca cggatatcga gcgcattgcc gaccaggcca gcgatattgc cgagatcacg
    12901 ctgcacctcc acccggaggg tgcgcgcacc gtcggtgtgc tggatgattt gtacgccatg
    12961 ggcgatatcg ccctgcggat ggtcaaggat tccattgccg cttacgtgca ggtggacctg
    13021 tccaccgcag cccaggtcat tgcccgcgac gacgagtgtg acgcgatgtt cagccgcatt
    13081 tcccaggaaa ttgccgccta catcgccgcc caccccgatg acgccagcac ggcgctggat
    13141 ctgttcatga tcgacaaata cctagagcgc ctgggtgacc acgcggtcaa tatcgccgag
    13201 tgggtcgagt tcttaaagac cggcgttcac aaatcccgca agatcgtgta acgcggggca
    13261 aaacaagcca ttcccttatt ttcctatacc aaccaaaatg gggcctgccg caaagcagac
    13321 cccattttgg cgttgaggga aaaggcttcc cccatggggg aagctgtcac cgaaggtgac
    13381 ggatgagggc aagccttgcc gaggctgcgc gcaaacagac tgtggccgtg aacttccccc
    13441 tcatccgttt gaggtcgggg ccgcagacat cttcccccaa agagggaaag cacagactgc
    13501 caaagacttc cccgcaaaaa atcccgggca gctcacggtg agccgcccgg gattgtgctt
    13561 ttatttcgcg ccgcgccccg cgaaaaccac gtacacggtc ttgaacaaaa tctttacgtc
    13621 gttccagagc gaccagttat caatgtaggt cacatccagc ttgacgactt cctcaaagtc
    13681 ctcgatgttg ctgcggccgc tgacctgcca caggccggtg atgcccggct tcatcgacag
    13741 gcggcgcttg tggtggctct cgtactgctt gtactcatcc accgtcggcg ggcgggtgcc
    13801 cacgaggctc atgtcgccgc gcagcacatc aaagaactgc ggcagctcgt cgatgctcgt
    13861 cttgcggatg aacttgccca ccttcgtcac gcgggggtcg tcctccattt tgaacatcag
    13921 gccgttcatt tcgttcttgg ccatcagctc tttcttgcgt tcctcggcat ccatgtacat
    13981 actgcgcagc ttgtggatgt agaaataccg gccgttcagg cccacgcgct tctgcttgaa
    14041 gaagagcggc cctgggcttt cgatcttcag cgggatggcc accaccgcaa tgatgggaat
    14101 gctgatggcg cagcccacaa tggcaccggc aatgtccatg gcgcgcttgg caacgcggtc
    14161 ccgcagcttc atttccacgg tgcccatcgt cacgatgttg ccccccgcgc aacggcccag
    14221 cgtgcggctc agccgtgccg agctcgtctc gtcgcagcag cgcgcctcga tatggtccag
    14281 cagcggcagc cggaaatgca cggtcaagcc catgctttcc atctcttcca ggtaggggat
    14341 gaagctctcg ccgctgtcca tcggcacgtc cacatagacc tcgtccagcg cctcgcgccg
    14401 cagccaggcc atgaaatcat caaacgttgc cttgacctcg accccggcca ccctggtgcc
    14461 gacggtctcg ggtgtggctt ccagcagggc tacgccgatg atacggcggg accagtcgcc
    14521 ctggatctcg gcgatgagct tttcggcgcg gtcacaggtc gtcacaaagc ccacaaggct
    14581 ctccgacccg cggctgtgct gcaatttcag caggaacttc ttcagcagcc catgggtgat
    14641 gggcatgagc accacgttca caatggggac catgaccgca aaatagcggg agtccagcag
    14701 cgtggctttc gccagcacca ggcaggccgc attgacgacg ccgagcacga cgttgaattt
    14761 cagtgccagg atgatttcgt cgcccttgga gcgggtcacg atgttctgat cctggtcaaa
    14821 gcacaaaaac gtcagcacaa aggccagcag cagcaggcaa agcgcctgga tccagtcaaa
    14881 ggttgcgtaa tcctgcagct tgttgaacac gttatccgtg ataagccatg ccagcacaac
    14941 actgatgccc gtggtcacaa aatcggtgag aaacacatac gcgtactgta atttttcacg
    15001 tttgctcgtc atatcggtga agattcctct cgtatatagt ggcagatgcc gcgcatgata
    15061 gcggcggcat acaatgcaat gacacacata tcactgtatt ttatcacaaa acgagacaat
    15121 tttcaacata gttccgctga agcgtaataa atctgtaaga aatcgggaaa taagcggcgt
    15181 gtgtttgtag ggggcggagc agaactccgc ccctatcgtg ttgtaaggtg gccggtcctc
    15241 tggccgccgc aggcattatt ctgcctcggt aacatccgct ttcagcacgt cctgtatctc
    15301 caccagacat tccagcagca ggggattgaa cgtgccgcag tcgccgtgca aaatcatctg
    15361 cacagcggtg tcgggggcgt aggcggcctt gtacacgcgc ttgctcacca gcgcgtcgta
    15421 cacgtcggcc atcgagacga cctgcgccga aatcgggatg cggtccccgg acaggccgtc
    15481 agggtagccg cggccgtccc aacgctcgtg atgccaacgg cagatttcat acgcggtgcg
    15541 caccagtgct tcgctgcggt atatgcccag tttgtccagc atatttgcgc cgatgaccgt
    15601 gtgggtcttc atgatgttga actcctcctg cgtcaggcgg ccgggcttgt tcaaaatctt
    15661 gtcgtctatc gcgattttgc cgatgtcgtg cagcgccgat gccagcacga tcagctcctg
    15721 cttctcgggc ggcaggtcgt acttgtcggt tttctgggtc aggtgctcca acaggcgctg
    15781 ggtcagcgtg ccgatgtgct gcacgtgcag gccgctctcg ccgttgcgga actcaacgac
    15841 ctcgctcaaa atcgagacca tcatctgggt gttcttctct ttctcgcggg tctggctcgt
    15901 caccagcgag atgagccgcc gctgcttggc gtagagcttg atggtgttaa agacgcgccg
    15961 gtagacgatg cttgcgtcaa aggggcggct gacatagtcc gagacgccca tctcgtaggc
    16021 gtgacgcacg accgggtcgg agtcctcgct ggaaatcatg atgaccggga tatcctcgat
    16081 ccagtggttg cggttcatgg cagccagcac ttcaaagccg tccatcaccg gcatgacgat
    16141 gtccagcaga atcagcgcaa tgccggtgtc gtactgctcc agcatggcca ggcattcctt
    16201 gccgttggcg gcctccaata tattgtagtc gctgcccaga atctccgaca aaatcgcgcg
    16261 gttcatctcg gaatcatcaa cgatcaggat gttctggcgg gtctggttct cgcgcccggc
    16321 ggcggtgctg gccctggggg cggcggtgtc ctcggtcacg accgtgtttt tgtggttctt
    16381 ggcctggtac atcagcttgt cggcgcggcc aacggcgtgc tccaccgttt cgtccggctg
    16441 ggacatcacg ccgccgatgc tgaccgaaag ctgcagacag ctgtaccccg gcacggtggc
    16501 ggcgtggatt ttcttgcaga tgcgctgtaa ttttgcgttg aacgcgcccc gctcaatgcc
    16561 cggcagcacc agcagaaatt cgtcgccgcc gtagcggatc aggctgtcgc ttttgcgaat
    16621 gttggcgcgc acgacctcca tggcggtgcg cagggccaga tcgcccgcgt ggtggccgta
    16681 ggtatcgttg tagagcttga agtcgtccag gtccagcacg gccacaccgg cagggtcggt
    16741 cttgtccttg agcacatcct caaaataacg gcggttgtag ccgccggtca gggcatcgtg
    16801 gtagagcttg aagtggtagc cggtcacgct gtccatcagg cggttgctgt tttcggggtc
    16861 gataagggtt tcgctgtcca gctgctggat cagctccatc acgcagggct ggccgtccac
    16921 ctcacggtag accgccgtga cctgaaagac ctggctgccc atgtattcca gcttggtgcg
    16981 ctgggtgtgg tcacgcagga cctgcgccga gatacagttc tggcactggg tgtgcttctg
    17041 ccaaaagtcg tagcatttgc agtagtcgct gagggtcttg tcactgccga cctgcgcagc
    17101 ctccaacagg cgcacgaccg taaaaatcgt gcggtattgg gccatttcct tttcgacctg
    17161 ttctctggtc atattctgcc gccttttgtg gatttattgt gttatatact acagcagtat
    17221 aacatcgtgc ggcgaaaaat gtcaatttat ttgtggcgat gtcgtacata accgtgggca
    17281 gcgccgcata ggcttgtacc gtgagggggg ggacgaccta tgcgccgagt tgtattctat
    17341 ctatttataa tagctgtttt tctggcggcc ccgctggcag cgctgccccg gcggggggag
    17401 actgcgcccg ctgccacccc tgcgcccacg gccacggcag agccaaccgc cgccgcgccc
    17461 taccgcgccg tgtgggtcag ttacctggaa tggcagcagg tggatttttc cgcgacggag
    17521 acgttttctg acgatatagc ggtcatactg gacaatatcc ggaacctggg ggccactgtc
    17581 gtgctggcgc aggtgcggcc ctttggggat gcactctacc ccagcagcta ctacccattc
    17641 agccaccttt gcaccgggca gcaggggcgg gaccccggct atgacccgct ggccctgctg
    17701 gtgcaggccg cccacgaccg ggggcttgca ctggaagcct gggtcaaccc ctaccgcatc
    17761 caggctgggc tgaccccggc gctctgcgac gccagcccag ccaagctgca ccccgattgg
    17821 gtcaaataca ccgataccgg cgcctacctg gacccggcca acacggcggt gcggcaggac
    17881 atagccgatg ctgtgggaga gctgtgcgcc aactatgatg t
//
LOCUS       DBMDSQ010000010        17333 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_1052_length_17333_cov_2.300429, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000010 DBMDSQ010000000
VERSION     DBMDSQ010000010.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 17333)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 17333)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17333
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1052_length_17333_cov_2.300429"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            425..1204
                     /locus_tag="ACZPAX_00780"
     CDS             425..1204
                     /locus_tag="ACZPAX_00780"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007048019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HIR1112463.1"
                     /translation="MILALNIGNSNITFGGYTPDGKLVFSSRLFADTALSSDELLYKI
                     VNMLALYGAEPQQITAVIFSSVVPALTPRLREALRKMCECQIMEVGPGLKSGVRIRMD
                     NPAQLGGELLCAIVGALQHCTPPCVVVNFDTATTLLAVDTTGALVGGAILPGPQCSLG
                     ALIRNTAQLPQVELEARPRKLLGTNTADCLHSGIVYGTAAMLDGMVAQIRGMLGASEA
                     PVVATGTLPDSVRAACTTEIHYRETLILEGLYVIWKKNARK"
     gene            1393..3279
                     /locus_tag="ACZPAX_00785"
     CDS             1393..3279
                     /locus_tag="ACZPAX_00785"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013080.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HIR1112464.1"
                     /translation="MQHFKQINWKKFFLQRVLPCLLAVAVGFISRYQLELSDGVTPSF
                     LELQWPLYAPLNALTAFCLTLILFAVLGKWSCATGISGAVFTVIALINYYTRDLHGSA
                     LMPQDILNLGTAAEVMGSYTLKITKDVVTIALLYLPILAIAFVQAQLVKGAPKHASWK
                     MRGVRVAGSALGVFLVMFFGYFGPVTIKPKTTYGWAWQNTYYTYGYLAGTIEATSLMS
                     DPIIEPENYTDTAVQTAAQKAGDYVAPASPESAEDYPDVVLILSESFYDFDLVTDLQA
                     DTDIMPVTKNLPNSVYGHTISPHVGGGTNSTEYEMLTSNSLILMPSITPFNWLNLYNA
                     NSVVSYLKGLGYSTMAAHPYTNSNYRRDSAWLAMGFDETHFQDDFTSQEYYGSRPYQT
                     DSATYRDWEKMYEAMPEDKPRFSFLVSIQSHGDYDMNDASLDIVHAGTDYGEYDELMN
                     EYLSCIKMTDAAVQELCDYFTEQYEKTGRKVIVAMAGDHAPSFVKHVADASFAATNND
                     LQILERSTPFFIWANYPLEHTAAATSTTDPLNRMDMVMLAPTLLQQAGLPLSDYYKYL
                     LEMKHNTPVVTAANDYMKVDGTTAEYGTDPALDEWAKGYLALEYNNIGVHAKRDQSIF
                     TPAE"
     gene            3295..4005
                     /locus_tag="ACZPAX_00790"
     CDS             3295..4005
                     /locus_tag="ACZPAX_00790"
                     /inference="COORDINATES: protein motif:HMM:NF019575.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HIR1112465.1"
                     /translation="MIGLRRTLRDRRGFTLVELVVVLVILGITASFAVPALTGYIDTS
                     KEKKAVSEAQACVMAATRLGAAQYAKVQQHNVNVSLNSGAGAAKDDSLDNWAPVDGQS
                     APEITGGDVALTEGSGQYLLHVTDAPAGSFPGDVSAFTTAAEVDGSVKTLTFNSNGQL
                     LYLVYTSTDGIQVVYTNSGSKASVKNDDDFVAVPTPKATDAPTGQPDEEKSYTGTDKE
                     NKVHKNWHQVGKNKYTIE"
     gene            complement(4156..4905)
                     /locus_tag="ACZPAX_00795"
     CDS             complement(4156..4905)
                     /locus_tag="ACZPAX_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007048768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HIR1112466.1"
                     /translation="MIQNTILSALTARKSVRVFTGDPITADERGAILHAAFQAPTAGN
                     QQLYTILDITDPALKATLADLCDHQPFIAKAPLVLVFLADCRRWLNAYHAAGIEDVRK
                     PGAGDLMLAMADTCIAAQNAVVAAESMGIGSCYIGDVIENVEAMRDALHLPDFVVPAC
                     MLVFGRPTEQQQRRPKPARFAEAAVVCENTYRDRTPDELRADFTARAAANGQPDYDFD
                     KAVTAFCNRKYASDFSQEMTRSAEIYWKEFE"
     gene            complement(5026..6456)
                     /locus_tag="ACZPAX_00800"
     CDS             complement(5026..6456)
                     /locus_tag="ACZPAX_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007048767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140327 - flippase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HIR1112467.1"
                     /translation="MPSPQKKYTTLVSNTLLFAISNFSSKLLSFFIRPYLSYALDSPD
                     VMGVSSLLQQATNLLIPVVSLGVAYAVIRFGLDKETDKSSVFVNGAATISLGFVVLLL
                     AMPLVRLIPNAAEYLGFLYLCVLASCLRTLCTQFIRARMLNRLVAIDGVLTTLSLLLY
                     YLLFLSVFKMGATGFLLANALADLTSMVFVFIAGGCWRYFKPKAFNTALWKDMLRYCL
                     PMIPASISFWIINASDMFFVQGMCENYGGRSGNHWVGLLSAGYFLPQIITILGSIFYE
                     AWQLSAVTEETDREAFFSKIFRVYASVLFCCVAGIIMLCQVLMRVFKAEYFDAWTFVP
                     FLTLCSMLTCLNQFMNSIYVVYKRSTGSLFTMLAGAVLNLILNYLFILHWGPWGVTLA
                     SFLSLMLVFLLRAYSTRGLLEIDFHPAWLVLNLALVLGEIWCMMNLERWALPVVGITA
                     VVCVMNIREVLSMLDTLLAKFVKKKK"
     gene            complement(6671..7237)
                     /locus_tag="ACZPAX_00805"
     CDS             complement(6671..7237)
                     /locus_tag="ACZPAX_00805"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005938909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HIR1112468.1"
                     /translation="MAAQKVQQRTAGGIAYTLTRRRVRNINLRVRADGSVAASASVRV
                     PVSYVDAFVAARADWVRSAQARAAVRREREEAERPELPAKADALAHMQALCRVYYPQF
                     AASCPGGRMPKIAVRDMTTRWGSCSLRTGTLAFARRLCVMPLAAQEYVVVHEFCHFAH
                     PDHSPAFWAAVARVMPDYKQREKLLKMR"
     gene            7426..8757
                     /locus_tag="ACZPAX_00810"
     CDS             7426..8757
                     /locus_tag="ACZPAX_00810"
                     /inference="COORDINATES: protein motif:HMM:NF012415.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HIR1112469.1"
                     /translation="MKSTHQTLLALSVSCVLAASLAGCGVQITSVALDLPDSLEKGAV
                     ITAEPEYIFDGATPESAALAKKLDALGIGYVSSDPTVVMVDDFGNLAALKAGTAEVAL
                     SSQDGTLTASKTIEVVVTPTGITTTDTLTLTTGETAALETAVAPEDATHVAITYTSSD
                     DAIATVSDAGEVTAVAAGEADVTAAVDGTELAAVCQVTVLPAIESVELSDTALTLQPE
                     ETAQLTYTVAPEEALADNVTYTSSDETVATVDAEGNVTAIADGTATITVDVNGVTAEC
                     EVTVATKAASTVGNSNNGSSAASSGQTQDSSAASSSSFEYGAIPFSNANDGTWWYIDQ
                     ADSPYWAVLNNINAMRAAGGLSALSASSSLDSIAYSRCDYQLKNNTLSHDGAQTPEIL
                     AQSQKSASEVCSGWQNSPSHYAVIMNPNFTQIGICCYFEIGGKTIWCCTFG"
     gene            complement(9077..9151)
                     /locus_tag="ACZPAX_00815"
     tRNA            complement(9077..9151)
                     /locus_tag="ACZPAX_00815"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9116..9118),aa:Glu,seq:ttc)
     gene            complement(9243..9319)
                     /locus_tag="ACZPAX_00820"
     tRNA            complement(9243..9319)
                     /locus_tag="ACZPAX_00820"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9283..9285),aa:Met,seq:cat)
     gene            complement(9492..10799)
                     /gene="serS"
                     /locus_tag="ACZPAX_00825"
     CDS             complement(9492..10799)
                     /gene="serS"
                     /locus_tag="ACZPAX_00825"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007048479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HIR1112470.1"
                     /translation="MLDIRFVRENPDAVKENIKKKFQDAKLPLVDEVIKLDAEYRAAI
                     GEASDLRANRNKLSKQIGALMGQAKKDPSKAAEAEEIKKQVTAQADRLKELEAKETEL
                     QAKIQEIMYTIPQMIDPSVPIGPDDSHNVEVQRFGEPVVPDYPIPYHVDIMESFDGID
                     LDAAGRVSGNGFYYLLGDIARLHEAVLAYARDFMIDKGFTYVIPPFMMHGDVVKGVMS
                     FPEMDAMMYKIEGEDLYLIGTSEHTMIGRFIDQTLDGAKLPLTLTSYSPCFRKEKGAH
                     GIEERGIYRIHQFEKQEMIVVCKPEDSMDWYDKLWKNSVELFRSMEIPVRQLECCSGD
                     LADLKVKSCDIEAWSPRQQKYFEVCSCSNLGDAQARRLRIRFKDENGKTQLAHTLNNT
                     CVAPPRMLIAFLENHLQADGTVTIPEVLRPYMGGKAVLVPNKK"
     gene            complement(11126..12004)
                     /locus_tag="ACZPAX_00830"
     CDS             complement(11126..12004)
                     /locus_tag="ACZPAX_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007048480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HIR1112471.1"
                     /translation="MAKKKLGGLGKGLDSLFAELPDISDEPSSSTLPLREIEPDPEQP
                     RKRFDEDALNQLADSIAENGLLQPIAVRPKKVGTGYIIIAGERRWRAARLAGLDEVPV
                     IIKDVTDEQAAALALIENLQREDLDPIEVAEGCRQLIDKYGLTQESAAKKLGKSRSAV
                     TNSLRLLALPEDVRRMVSAGKLSFGHAKVLLGLPSAELMQQAAAEVVAQNLNVRQTEA
                     LCKKLAKPAKPPKEKDNFTRPKRAVEVEAALKEVTGSEVHVEYKDGKGSLKIDFYSDD
                     MLQKFVSLLGMYDPEG"
     gene            complement(12017..12781)
                     /locus_tag="ACZPAX_00835"
     CDS             complement(12017..12781)
                     /locus_tag="ACZPAX_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007048481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HIR1112472.1"
                     /translation="MAKVIAIANQKGGVGKTTTAVNLSACVAALGKKVLIVDLDPQGN
                     TTSGYGISKNKVEADTYTCLIDDEDVRGAIVKTEYNADLLPSNTQLAGAPIELVSVPR
                     REMRLRTVLAPVVPLYDYIFIDCAPSLDLLTVNALCAADTVIIPMQCEFFALEGLSEL
                     MTTLKTVRKKYNRYLDIEGVLFTMYSGRLNLTMQVVAQVKKYFGDKVYRAVIPRTVRL
                     SEAPSFGMPINFYEPNGKGCDAYMELAREFLANNER"
     gene            complement(13072..13938)
                     /locus_tag="ACZPAX_00840"
     CDS             complement(13072..13938)
                     /locus_tag="ACZPAX_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007048482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HIR1112473.1"
                     /translation="MPKNDIQKQGRILMLPVDCIEPSPYQARTAFDEPEIAALAVSIL
                     QNGLLQPISVRRVGIRKYQLVAGERRLRACRLAKLDKVPAILADYDDSESAALGLLEN
                     LQRSQLDPFDTARGIKEVIRLWGCTQAEAARRLGLSQPALANKLRLLTLTPEQQQLCT
                     ANHLSERHARAALRLPEGRRTAALEKMARDKMSVRQADKLVDQMLTAPNAPSPCRRTI
                     PMVRDVRFFVNTLQHAVDLMTQKGIAATTHCDKHDGCLEYIVRIPVSSDGTVGKQEDA
                     PKKEDSEFAVKV"
     gene            complement(14119..14823)
                     /gene="rsmG"
                     /locus_tag="ACZPAX_00845"
     CDS             complement(14119..14823)
                     /gene="rsmG"
                     /locus_tag="ACZPAX_00845"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007048483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HIR1112474.1"
                     /translation="MIDKNRLATKCSTWNITLTPTQLDLLDKYAELLVSYNEKVNLTA
                     ITSPEGIEDRHFADSLLFAAQPEVQGKMVDVGTGAGFPGIVAKIYKPDLQLTLMEPTG
                     KRVDFLKYVCAELGLTGVEFAKERAEEAARKIWREQFDIASARAVAALPVLSEYCLPL
                     VKVGGRFIAMKGPEADAEAKAAANALKKLGGRYEETRQFTLPDGSARGLVFCKKISQT
                     PTVYPRNGGKIAKKPL"
     gene            complement(14994..16862)
                     /gene="mnmG"
                     /locus_tag="ACZPAX_00850"
     CDS             complement(14994..16862)
                     /gene="mnmG"
                     /locus_tag="ACZPAX_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005936991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HIR1112475.1"
                     /translation="MDKLGTYDVIVIGAGHAGIEAAHAAAKLGARTAVFTLSLDFIGN
                     MPCNPSIGGTAKGHLVREVDALGGLMGIAADATYLQSRMLNRGKGPAVHSLRVQTDRK
                     RYHIWMKHALEKTANLDIHQGEIVGVDVQDGKVCGVYTNLHGYYGCKAVVVATGTNLG
                     GRIFVGDAHYDSGPDGTHAATELTKCLVAHGFTLRRFKTGTPARVHRRSIDFSKLECQ
                     PGDPDDELQPFSFMTRVPMHNKVNCYIAYTNPATHKVILDNLHRSPLYGGDIQGVGPR
                     YCPSIEDKVVRFKEKERHPVFVEPCGEDTEEMYLQGLSSSLPEAVQNEMYRTIAGFEN
                     LEIMRPAYAIEYDCVDPTTLKPTLESKVVSGIYGAGQFNGTSGYEEAAAQGLLAGLNA
                     ARHALGKEELVLARHTSYLGTLVDDLVTKGVMDPYRMMTSRSEYRLSLRQDNADERLT
                     PIGREYGLVDDERWAMFCHDREVKQTELHRLETTSIKLADLRAAAPEGAELGEIGGTA
                     IELMRRPYINYELVAKVIGRGEGVTTAMAQRIETEIRYAGYIAREERIIRDIQRHENV
                     KIPEDFDYAPIETMTLESREKLAKIRPRTLAQAGRIPGVSPSDLAQLSIVILKDSK"
     gene            complement(16875..17093)
                     /locus_tag="ACZPAX_00855"
     CDS             complement(16875..17093)
                     /locus_tag="ACZPAX_00855"
                     /inference="COORDINATES: protein motif:HMM:NF016370.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease domain-containing protein"
                     /protein_id="HIR1112476.1"
                     /translation="MIGNFIVDFYIPSCKLVIELDGAQHTEPQAAEYDAHRTAYLQSI
                     GCRVLRYGNNEMDTNFAGVCEDILQKIG"
BASE COUNT         3459 a         5156 c         5084 g         3634 t
ORIGIN      
        1 taaaggcaat gtgcggcgga gggtcaagcc ccctccctac aatatggaag attaaattgc
       61 agtaacggca aggtggaggg ccgggcatgc ccggccccta cagggggacg gaaggtaagg
      121 ttggcgatag tggcagggtg cgcgggcggc atgtatgccg cccctacatg ggcgacctgg
      181 cgggagtaag gggcaataaa ggcaatgtgc agcggagggt caagtcccct ccctacaata
      241 tggaagatta aattgcagta acggcaaggt ggagggccgg gcatgcccgg cccctaccgg
      301 gtggtaaatg ggtaagtata cgctatatgt ttcaacattg ttcggctaac gttgtggaaa
      361 agtcttgcaa tttgtacctt attataatag tataaagaca atatgtttga aatgaggttt
      421 tcccatgatc ctggcattga atattggcaa ctccaacatt acgttcggcg ggtatacgcc
      481 ggacggcaag ctggtgtttt cgtcgcggct gtttgcggac accgcactga gcagcgatga
      541 gctgttatat aaaatcgtaa atatgctggc cctgtatggg gcggagccgc agcagatcac
      601 agcggtcatc ttttccagcg tggtgccggc gctgacgccg cgcctgcgcg aggccctacg
      661 caagatgtgc gagtgccaga tcatggaggt cggccccggg ctgaaaagcg gggtgcgtat
      721 ccgcatggat aaccccgccc agctgggcgg cgagctgctg tgcgccatcg tgggtgcgct
      781 gcagcactgc acgccacctt gtgtggtggt gaactttgac accgccacca cgctgctggc
      841 cgtggatacc accggtgcgc tggtgggcgg cgcgattttg ccagggccgc agtgctcgct
      901 gggggcgctg atccgcaaca cggcgcagct gccccaggtg gagctggagg cccgcccccg
      961 caagctgctg ggaaccaaca cggcggactg cctgcacagc ggcatcgtgt acggcacggc
     1021 ggccatgctg gacggcatgg tggcgcagat acgcggtatg ctgggggcca gcgaggcccc
     1081 ggtcgtggcg acaggcacgc tgccggacag tgtgcgtgcg gcctgcacga ccgaaataca
     1141 ctaccgtgag acgctgattc tggaaggttt gtatgtgatt tggaagaaga atgcgcggaa
     1201 gtgagttata aggctaaggc tggcggagca gagccccgcc cctacggttc gctaaaaggg
     1261 taagctgcaa taatgataag gctgagggcc gggcataccc ggcccctact ttatttttat
     1321 aaatgggtat gttataatgc actatgtata tttctgtaca aattcataga gaaaggtcat
     1381 ttatcatcac ccatgcaaca ttttaaacaa atcaactgga agaaattttt cttacagagg
     1441 gtactcccct gcctgctggc tgtggcggtc gggtttatct cccgctatca gctggaatta
     1501 tccgacggcg tcacgcccag ctttctggaa ttgcagtggc cgctctacgc gccgctgaac
     1561 gccctgacgg ccttctgcct gacgctgatc ctgtttgccg tgctgggcaa gtggagctgc
     1621 gccaccggca tttccggcgc agtgttcacc gttatcgcgc tcatcaacta ctatacccgc
     1681 gacctgcacg gttcagcgct gatgccccag gacatcttaa acctgggcac cgccgctgag
     1741 gtcatgggca gctacacgct gaaaatcacg aaagacgttg tgaccatcgc cctgctctac
     1801 ctgccgattc tggcaatcgc ctttgtgcag gcacagctgg tcaagggcgc ccccaagcac
     1861 gccagctgga agatgcgcgg cgtgcgcgtg gcgggcagtg cgctgggcgt gttcctcgtc
     1921 atgttcttcg gctacttcgg gcctgtgacc atcaagccca agaccaccta cggctgggcg
     1981 tggcagaaca cctactacac ctacggctac ctggccggaa ccatcgaggc cacgagcctg
     2041 atgtccgacc ccatcatcga accagaaaac tacactgaca ctgccgtgca gaccgccgca
     2101 cagaaagccg gtgactacgt ggcccccgcc agccccgaga gcgccgagga ctaccccgac
     2161 gtcgtgctga tcctttccga gagcttctac gactttgacc ttgtgaccga cctgcaggcc
     2221 gacaccgaca ttatgccggt cacgaaaaac ctgcccaact cggtctacgg ccacacgatc
     2281 agcccccacg tgggcggcgg caccaactcg accgagtatg agatgctgac ctccaactcg
     2341 ctgatcctga tgccgagcat cacgccgttc aactggctga acctgtacaa cgccaacagc
     2401 gttgtcagct acctgaaggg cctgggctac tccacgatgg ccgcccaccc ctacactaat
     2461 tccaactacc gccgcgactc tgcctggctg gccatgggct ttgacgagac gcacttccag
     2521 gacgacttca cgagccagga atactatggc agccgcccct accagacgga ctccgccacc
     2581 taccgggatt gggagaagat gtacgaggcc atgcccgagg acaagccccg gttcagcttc
     2641 ttagtctcca tccagagcca cggcgactac gatatgaacg atgcttcgct ggatattgtc
     2701 cacgcgggca ccgactacgg cgagtacgac gagctgatga acgagtacct gagctgcatc
     2761 aagatgaccg acgccgccgt gcaggagctg tgcgactatt tcaccgagca gtacgaaaag
     2821 accggccgca aggtcattgt tgctatggcc ggcgaccacg cgcccagctt tgtcaagcat
     2881 gtggctgacg cgagctttgc cgccaccaac aacgatttgc agattttgga gcgcagcacg
     2941 ccgttcttca tctgggcgaa ctacccgctg gagcacacgg ccgccgccac cagcaccacc
     3001 gacccgctga accgcatgga catggtgatg ctggccccca ccctgttgca gcaggctggg
     3061 ctgccgctga gcgattatta taagtatctg ctggaaatga agcacaacac gccggtcgta
     3121 accgccgcca acgactacat gaaggtggac ggcaccacag ccgagtacgg cacagacccc
     3181 gcgctggacg agtgggccaa gggctacctg gcgctggaat acaacaacat tggtgtgcac
     3241 gccaagcgtg accagagcat ttttaccccc gcagaataac tgggaggaat ccgtatgata
     3301 ggtcttagac gaaccctgcg cgaccgccgc ggctttacgc tggtggagtt ggtggtggtg
     3361 ctggtgattt tgggcataac ggcgagcttt gccgtgcccg cgctgaccgg gtacattgac
     3421 accagcaagg agaaaaaggc cgtcagcgag gcgcaggcgt gcgtgatggc ggcgacccgg
     3481 ttgggtgcgg cccagtatgc caaggtgcag cagcacaatg taaatgtatc gctcaacagc
     3541 ggcgcaggcg cggcaaagga tgacagcctg gacaactggg cacctgtgga cgggcagtcg
     3601 gcacccgaaa tcaccggcgg tgacgtggcc ctgaccgaag gcagcggcca atatttgctg
     3661 cacgtaacgg acgcaccggc aggcagcttt cccggggatg tttcggcctt tacaacggcg
     3721 gccgaggtgg acggcagcgt caaaacgctg accttcaaca gcaacggcca gctgctctac
     3781 ctggtctaca ccagcaccga cggcatccag gtcgtctaca cgaacagcgg cagcaaggcc
     3841 agtgtgaaga acgatgatga ttttgtagcc gttccgacgc cgaaagcgac ggatgccccc
     3901 acaggccagc cagacgaaga aaaatcttat acaggaaccg acaaagaaaa taaagtccac
     3961 aaaaattggc accaggtcgg caaaaataag tacaccatag aataactctg tctaacgcca
     4021 caacgcccct tgcatggctg tcatgcaagg ggcgttgttt atttttctta ttttccaggg
     4081 tacggttcag cctgcggcaa acgcgccact catccggccc tttgggccac ctttccccta
     4141 aagggggaag gctttctact caaactcttt ccagtaaatt tctgcgcttc tggtcatttc
     4201 ctgggaaaag tcgctggcgt atttgcggtt gcaaaaggcg gtgacggctt tatcaaagtc
     4261 gtagtcgggc tggccgttgg cggcggcgcg ggcggtgaag tcggcgcgca gctcgtcggg
     4321 ggtgcggtcg cggtaggtat tctcgcagac gacggcggcc tcggcaaagc gggcgggctt
     4381 ggggcggcgc tgctgctgtt cggtcgggcg gccaaacacc agcatacagg cgggcaccac
     4441 aaaatccggc aggtgcagcg cgtcccgcat ggcctcgacg ttctcgatga cgtcgccaat
     4501 gtagcagctg ccgatgccca tagattccgc ggcgacgaca gcgttctgcg cggcgatgca
     4561 ggtgtcggcc atagccagca tcaaatcgcc cgcgccgggc ttgcgcacgt cctcgatgcc
     4621 tgccgcatgg taggcgttca gccagcggcg gcagtcggcc aggaacacca gtaccaacgg
     4681 cgctttggcg atgaacggct ggtggtcgca caggtccgcc agcgtggctt tcagcgccgg
     4741 gtcggtgata tccagaatcg tgtacagctg ctggttgccc gccgtgggtg cctggaacgc
     4801 tgcgtgtaaa atggccccgc gctcgtcggc ggtgatgggg tcgccggtga acacccggac
     4861 gcttttgcgg gcggtgaggg cggataaaat cgtattttgg atcatagaaa atctcctttt
     4921 ttataaacca gcaggggcaa ggcatacctt gcccccaacc ttgcctttat gctgtgctta
     4981 cggcaaagtt gagggccggg catacccggc ccctacaatc tctgcttatt ttttcttctt
     5041 cacaaacttc gccagcagcg tatccagcat gctcagcacc tcgcggatgt tcatgacgca
     5101 gaccaccgcg gtgatgccta ccaccggcag ggcccagcgc tccaggttca tcatgcacca
     5161 gatctcgccc agcaccagcg ccaggttcag caccagccac gcggggtgga agtctatttc
     5221 cagcagcccg cgtgtggagt aggcgcgcag caggaacacc agcatcaggc tcaggaaact
     5281 ggccagggtg acgccccacg gcccccaatg cagaatgaac aggtagttca gaatcaggtt
     5341 cagcaccgcg cccgccagca tcgtgaacag cgacccggtc gaccgcttat acacgacgta
     5401 gatgctgttc ataaactggt tcaggcaggt cagcatcgag cagagcgtca ggaacggcac
     5461 gaacgtccag gcgtcgaaat attcggcctt gaacacccgc atgagcacct ggcacagcat
     5521 gatgataccc gccacgcagc agaacaaaac gctggcatac acgcggaaaa ttttggagaa
     5581 aaacgcctcg cggtcggtct cctcggtcac ggcggagagc tgccatgcct cgtagaagat
     5641 gctgcccagg atcgtgatga tctgcggcag gaagtacccc gccgacagca ggccgaccca
     5701 gtggttgccg ctgcggccgc catagttctc gcacatgccc tgcacaaaga acatatcgct
     5761 ggcgttgatg atccaaaagc tgatggacgc cgggatcatc ggcaggcagt agcgcagcat
     5821 atccttccac agcgccgtgt tgaacgcttt gggtttgaag taccgccagc atccgcccgc
     5881 gatgaacaca aacaccatgg atgtcaaatc ggccagcgcg tttgccagca aaaagccggt
     5941 cgcgcccatt ttaaacacgc tgaggaacaa gagataatac agcagcagac tcagcgtcgt
     6001 cagcacgccg tctatcgcca ccagccggtt cagcatccgc gcgcggatga actgggtaca
     6061 cagcgtgcgc aggcagcttg ccagcacgca aaggtacaaa aagcccagat attccgccgc
     6121 attgggtatc agccgcacca gcggcatggc caacagcaac accacaaaac ccaggctgat
     6181 cgtagccgcg ccgttgacga agacgctgga tttgtcggtt tccttatcca ggccaaaccg
     6241 gatgaccgcg taggccacgc ccaggctgac caccgggatg agcaggttgg tggcctgctg
     6301 caaaaggctc gacacgccca tgacgtccgg cgagtccagc gcgtagctca agtaaggccg
     6361 gatgaagaag ctgagcagct tggagctgaa gttggagatg gcaaacaaca gcgtattgct
     6421 gacaagcgtc gtgtattttt tctgtggaga tggcaagata ggacctccct tttggatgtt
     6481 attggattgt aagggcgcgg catccccgcc gccgagcttt ggcttccccc tggggggaag
     6541 ctgccgcctg caggcggctg atgaggggca ggtctgccgc tgttgcccct ttcccgggcg
     6601 gcatccataa acgcgcccct cctccggcct tacggctacc ttccccccag ggggaaggct
     6661 ttcccggccg tcaccgcatt ttcaacaact tttccctctg tttataatcc ggcatgaccc
     6721 ttgctacggc cgcccaaaac gccgggctgt gatccgggtg ggcaaaatgg caaaattcat
     6781 gcacgaccac atattcctgc gccgccagcg gcatcacaca gagccgccgc gcaaaggcca
     6841 gcgtgccggt gcgcaggctg cacgaccccc agcgggtggt catgtcccgc acggcgattt
     6901 tgggcatacg tccgcccggg cagctggccg cgaactgcgg atagtatacc cggcaaagcg
     6961 cctgcatatg ggccagtgcg tctgcctttg cgggcagctc ggggcgctcg gcctcctcgc
     7021 gctcacgccg gacggcggcc cgggcctgtg cgctgcgcac ccaatcggcc cgtgcggcca
     7081 caaaggcatc cacatagctt accggcaccc gcaccgacgc actggccgcc acggaaccat
     7141 cggcccgcac gcgcaggttg atattgcgca cccgccgccg cgtcagtgtg taggcaatgc
     7201 cccccgccgt gcgttgctgc accttttgtg ccgccaaact gccgccccct tttttataaa
     7261 gaatatatgc aatattgtac acgcatttgc gccaaagtgc aagcaaaaag gcggacacgc
     7321 cgcccgcctg aaattttttc gcatttgtct taacttttcg ccgccgtgcc cctatataga
     7381 gagtgaacct actgataagc tgcaaacaga aaggacgtat ttgctatgaa atctactcat
     7441 caaacgttac ttgcactctc cgtttcctgc gtgctggcgg ccagtctggc aggctgcggc
     7501 gtccagatca ccagcgtagc gctggacctg ccggacagcc tggaaaaagg cgcggtcatc
     7561 accgccgaac ccgaatacat ttttgacggt gcaacgcccg agagcgccgc ccttgccaaa
     7621 aagctggacg cactgggcat aggctatgtg tccagcgatc ccaccgtcgt aatggtagac
     7681 gacttcggca accttgccgc gctcaaggcg ggcacggccg aggtagcgct gtccagccag
     7741 gacggcaccc tcactgccag caagacgatt gaggtcgttg tcacgccgac gggcattacc
     7801 accacggaca cgctgaccct gaccactggt gaaaccgccg cgctggaaac cgccgtggcc
     7861 ccggaggacg cgacccatgt tgccattact tacacttctt ccgacgatgc cattgccacg
     7921 gtcagcgacg cgggcgaggt cacagccgtg gcggcgggcg aagcggatgt caccgccgct
     7981 gtggacggca cggagcttgc ggctgtctgc caggtcacgg ttctgcctgc gattgaaagc
     8041 gtcgaattga gcgacaccgc gctgaccctg cagcccgagg aaacggcgca gcttacctat
     8101 accgtagccc ccgaagaagc ccttgccgac aatgttactt ataccagcag tgacgaaacc
     8161 gttgccacgg tggacgcgga gggcaatgtc accgctattg ctgacggtac ggccactatt
     8221 acagttgatg taaacggtgt gacggctgaa tgtgaagtca cggtcgccac caaggccgcc
     8281 agcacagtgg gcaacagcaa taatggttct tccgccgctt ccagcggtca gacacaggat
     8341 tctagtgccg catcttcttc cagttttgaa tatggcgcaa ttccgtttag taatgcgaac
     8401 gatggaacgt ggtggtacat cgaccaagcc gattccccct attgggctgt actcaacaat
     8461 atcaatgcca tgcgcgcggc tggtggtctt tctgctttgt ccgccagtag cagtcttgac
     8521 agcattgctt atagtagatg tgactaccag ttaaaaaata atacgttgtc ccacgatgga
     8581 gcgcagaccc ccgaaattct cgctcaaagt caaaagagtg catccgaagt ttgtagcggt
     8641 tggcaaaaca gccccagcca ttacgccgtt attatgaacc ccaattttac gcaaattggt
     8701 atctgctgtt attttgagat tggcggtaag accatttggt gctgcacgtt tgggtaagag
     8761 gaatatcctg attttaaatc tagtataact ataccgcaag taatttcaaa aattcactat
     8821 atatcatcat atttttagca gagcaaaaat aaaaagcacc aaaatctaac aaaacgctaa
     8881 attctggtgc tttttggtga ccctcccgtc catctcccgc gccctaaaaa cagcccgcag
     8941 ggctgttttt gccctgcggg acgcggacgc agggcccggg cttttcgatt cccgtatggg
     9001 tcaccattta cgcccgacac ctgttgaaaa gtaaatataa agaaaaaagc actacctcaa
     9061 atgaagtagt gctttttggt gacccgtacg ggaatcgaac ccatgttaca gccgtgaaag
     9121 ggccgtgtct taaccgcttg accaacgggc catataatac aagatttcgc taaaacagca
     9181 agcaacttat cggctactat tgcaaccgac ccccaagtat tttagcgaaa ccttgtatca
     9241 tgtggtagcg gtaactggat ttgaaccggt gacacttcgg gtatgaaccg aatgctctag
     9301 ccaactgagc tataccgcca catttgtgag ctgcgctctc gagaacgcta tattattata
     9361 gccgacagtg ccccctatgt caagtagttt tccccgaaaa aattcaaaaa atacaaaaaa
     9421 aatcccacat tgcgggtgca atgcgggaaa aactctaaag gcttccccaa aaggggaagc
     9481 ctctttcaaa attacttctt gttgggcacc aacacggcct tgccgcccat gtaggggcgc
     9541 aggacctcgg ggatggtgac ggtgccgtca gcctgcaggt ggttctccag gaaggcaatc
     9601 agcatacgcg gcggggccac gcaggtgttg ttcagcgtgt gggccagctg ggtcttgcca
     9661 ttctcatcct taaagcggat gcgcaggcgg cgggcctggg cgtcgcccag gttggagcag
     9721 gagcagacct caaagtactt ctgctggcgc gggctccaag cctcgatatc gcagctcttg
     9781 accttcagat ctgccagatc gccggagcag cactccagct ggcggacagg gatctccatg
     9841 gagcggaaca gctcgacgga gttcttccac agcttatcgt accaatccat gctgtcctcg
     9901 ggcttgcaga cgacgatcat ctcctgcttc tcgaactggt ggatgcggta gatgccgcgc
     9961 tcctcgatgc cgtgggcgcc cttctccttg cggaagcagg ggctgtagct ggtcagggtc
    10021 aggggcagct tggcgccgtc cagcgtctgg tcgatgaagc ggccaatcat ggtgtgctcg
    10081 gacgtgccga tcaggtacag atcctcgccc tcgattttgt acatcatagc gtccatttca
    10141 gggaaggaca taacaccctt gaccacatcg ccgtgcatca tgaacggcgg gatgacatag
    10201 gtaaagccct tgtcgatcat gaaatcgcgg gcgtaggcca gcacagcctc atgcaggcgg
    10261 gcaatgtcgc ccagcaggta atagaagccg ttgccggaga cgcggccggc ggcgtcgagg
    10321 tcgatgccgt cgaagctctc cataatgtca acatggtacg gaatggggta gtcaggcacg
    10381 accggctcgc cgaagcgctg gacctcgacg ttatggctgt cgtcggggcc gatgggcacg
    10441 ctggggtcga tcatctgggg gatcgtgtac atgatctcct gaatcttggc ctgcagctca
    10501 gtctccttgg cttccagctc cttcaggcgg tcagcctggg cggtgacctg cttcttgatt
    10561 tcctcggcct cggcggcctt gctggggtcc ttcttggcct ggcccatcag tgcgccgatc
    10621 tgcttggaca gcttgttgcg gtttgcgcgc aggtcgctgg cctcgccgat ggcggcgcgg
    10681 tactcggcgt cgagcttgat gacctcatcg acgaggggca gcttggcgtc ctggaacttt
    10741 ttcttgatgt tttccttgac agcgtccggg ttttcacgga caaaacgaat gtctaacata
    10801 gtggtactcc tctattcaat atttgctttt gtaggggccg gacatatccg gccctcggct
    10861 ttacaaataa atagcatttt atgggtaggc tgcgggcggg gcataccccg cccctacaag
    10921 gctttcccct cggggctgcg cccgcaggcg gctgatgagg gaaaagttca tcttcattgc
    10981 gcgtcagcgg gtcattgccg gaaacttgcc cctcatccgg cctgcggcca ccttcagtct
    11041 acgcgctaag agccgcctac ggcggttgcg ctccgacacg cgcctgcggg cgcagcccca
    11101 agggggaagg ctttataacg gctgctcacc cttccgggtc atacattccc agcaggctca
    11161 cgaacttttg cagcatatcg tcgctgtaaa agtcgatttt cagcgagcct tttccgtcct
    11221 tgtattccac gtggacctcg ctgccggtca cctctttcag ggcggcttcg acctcgacgg
    11281 cacgcttcgg gcgggtgaag ttatcctttt ccttcggcgg cttggcgggc ttggccagct
    11341 ttttgcacag ggcctcggtc tggcgcacat tcaaattctg tgcgacgacc tcggcggcgg
    11401 cctgctgcat cagctcggcg ctgggcaggc ccagcagcac ctttgcgtgg ccgaagctca
    11461 gcttgcccgc gctgaccatg cggcgcacat cctcgggaag ggccagcagg cgcaggctgt
    11521 tggtgacggc gctgcggctt ttgcccagct ttttggcggc gctctcctgg gtcaggccgt
    11581 acttgtcgat gagttgtcgg cagccctcgg ccacctcgat ggggtccagg tcttcgcgct
    11641 gcaggttttc gatcagtgcc agcgcggcgg cctgctcgtc ggtcacatcc ttgatgatga
    11701 ccgggacctc gtccaggccc gccagccggg cggcacgcca gcggcgctcg cccgcaatga
    11761 tgatgtaccc ggtgcccacc tttttaggcc gcaccgcgat gggctgcagc aggccgtttt
    11821 cggcgatgct gtcggccagc tgattcagtg catcctcgtc aaagcgtttg cggggctgtt
    11881 cggggtccgg ctcgatttcc cgcagcggca gggtcgagct ggacggctca tcgctgatat
    11941 cgggcagttc ggcaaacagg ctgtccagcc ccttgccaag cccgcccagc tttttttttg
    12001 ccatgggcgt ccctccttac cgttcgttgt tggctaaaaa ttcccgcgcc agttccatgt
    12061 aggcgtcaca gcccttgccg ttcggctcat agaaattgat gggcatacca aagctgggtg
    12121 cctcgctcag gcgcacggtg cgggggatga cggcgcggta gaccttgtcg ccaaagtact
    12181 ttttgacctg cgcgacgacc tgcatcgtca ggttcagccg cccggaatac atcgtgaaga
    12241 gcacgccctc gatgtccagg tagcggttgt actttttgcg cacggtcttt aaggtcgtca
    12301 tcagctcgct caatccttcc agcgcgaaga actcgcactg catcgggatg atgaccgtgt
    12361 ccgccgcgca gagggcgttg acggtcagca ggtcgagcga cggtgcgcag tcgatgaaga
    12421 tgtagtcgta gagcggcacg accggtgcta agacggtgcg cagacgcatc tcacgccggg
    12481 gcaccgagac aagctcgatg ggagcacccg ccagctgggt gttggagggt aaaagatccg
    12541 cgttgtactc ggttttcacg atggccccgc ggacgtcctc gtcgtcgatc aggcaggtgt
    12601 aggtgtcggc ctcgaccttg tttttggaaa ttccgtaccc ggaggtggtg ttgccctggg
    12661 ggtccagatc cacgatcagg accttcttgc ccagcgccgc cacacaggca ctcaggttga
    12721 cggcggtggt ggttttgcca acgccgccct tctggtttgc tattgcgatg acttttgcca
    12781 cgccgtacct cccctatttt tgtgtttttt gggcggcaca gggccgcttg gtattattat
    12841 agcacaaaaa ttggggataa aaaaggggtt aaacagaaaa aagccccggt aaatgttcca
    12901 cgtggaacat ttaccggggc aaaggcttct cccttgggag aagctgtctg cgaagcagac
    12961 tgatgagggg cggccttggc tctactgccc tctttaccga gcggcaccct tacgccgccc
    13021 ctcatccggc ctttggccac cttccctcta agggggggag gcttttcttc ctcacacctt
    13081 caccgcaaac tcgctgtcct ccttcttggg ggcgtcctcc tgcttgccta cggtcccgtc
    13141 actgctgacc ggtatcctca ctatgtactc caaacatccg tcatgcttat cgcagtgcgt
    13201 cgtggcggct atccccttct gggtcatcaa atctaccgcg tgctgcagcg tgtttacaaa
    13261 gaaccgcaca tcccgcacca tggggatcgt gcggcggcag gggctggggg cgtttggcgc
    13321 ggtcaacatc tgatccacga gcttgtccgc ctggcgcaca ctcattttgt cccgcgccat
    13381 cttttccagc gcggccgtgc ggcggccctc gggcaggcgc agggcggcgc gggcgtggcg
    13441 ctcactcaaa tggttggccg tgcaaagctg ctgctgttcc ggcgtcaggg tcagcagccg
    13501 cagtttgttg gcaagggctg gctggctcaa acccagccgc cgcgcggcct cggcctgggt
    13561 gcagccccac aggcggatga cctccttgat gccccgcgcc gtgtcaaacg gatccagctg
    13621 gctgcgctgc aaattttcca aaaggcccag cgcggcggac tcgctgtcgt cgtagtcggc
    13681 cagaatcgca gggaccttgt ccagcttggc caggcggcag gcacgcaggc ggcgttcccc
    13741 cgccacaagc tggtatttgc ggataccgac ccgccgcacg ctgatgggct gcagcaggcc
    13801 gttttgcaaa atgctgaccg ccagcgcagc gatttccggc tcatcaaagg cggtgcgggc
    13861 ctggtagggc gacggctcga tgcaatccac cggcagcatt aaaatacggc cctgcttttg
    13921 gatatcattc tttggcaatt tcggttcctc cccctgggtt tactgtatgc ctgccgcaaa
    13981 gctgagggcc gggtatgccc ggcccctaca aaagcggccc ataaaaatat gctatgcttt
    14041 tttctacaaa aagcatagca tattttatac gcccgggaaa gaaaaatcgt tcgcccggat
    14101 atccggtttt tcgcggtgtt acaacggttt ctttgcgatt tttccgccat ttcgggggta
    14161 aaccgtcgga gtttgcgata tttttttgca gaaaaccagt ccccgcgcac tgccgtccgg
    14221 cagggtgaac tgccgggttt cctcatatcg gccgcccagc tttttcaggg cgttggccgc
    14281 agccttggcc tcggcatcgg cttcggggcc cttcatggcg atgaaccgcc cgcccacctt
    14341 gaccagcggc aggcagtatt cgctcagcac cggcagggcc gccacggcac gggcactggc
    14401 aatgtcgaac tgctcacgcc agatcttgcg ggcggcttcc tcggcgcgct ccttggcaaa
    14461 ctcgaccccg gtcaggccca gttcagcaca gacgtatttc aaaaagtcca cccgcttgcc
    14521 ggtgggttcc atcagggtca gctgcaaatc gggcttgtag atttttgcca cgatgcccgg
    14581 gaaccctgcc ccggtgccga cgtccaccat cttgccctgc acctcgggct gggccgcaaa
    14641 cagcagactg tccgcaaagt ggcggtcctc gatgccctcg gggctggtga tggcggtcag
    14701 attgaccttc tcgttatagc tgaccagcag ctcggcgtac ttgtccagca agtccagctg
    14761 ggtgggggtc agggtgatgt tccatgtgga acatttggtg gctaatctgt ttttatcaat
    14821 cattttttca tctccttgca caaaggcttc ttcctttggg agaagctgcc gcccgcaggc
    14881 ggctgatgag ggcagactgt atgtggctgc ccggttgcgg gtgaaatcga tatactcggc
    14941 cctcatccgc cctcgggcgg ggcctcgggc accttccccc agagggggaa ggctcattta
    15001 ctatccttca aaatcacaat actcagctgc gccagatcac tgggtgatac cccgggtatc
    15061 cgcccggcct gggctaaggt acgggggcgg atttttgcca gcttctcgcg ggattccagc
    15121 gtcatcgtct cgatgggcgc atagtcgaaa tcctcgggaa ttttcacgtt ttcatgccgc
    15181 tggatgtccc ggataatgcg ttcctcgcgg gcaatgtacc cggcgtagcg tatttccgtc
    15241 tcgatgcgct gggccatggc ggtggtcaca ccctcgccgc ggccgatgac cttggcgacc
    15301 aactcatagt tgatgtaggg gcggcgcatc agctcgatgg ccgtgccgcc gatttctccc
    15361 agctcggcac cttcgggggc agccgcgcgc aggtcggcca atttgatgct tgtcgtctcc
    15421 agccggtgca gttcggtctg cttgacctcg cggtcatggc agaacatggc ccaccgctca
    15481 tcgtccacca gaccgtattc cctgccaatg ggcgtcaggc gctcgtcggc gttgtcctgg
    15541 cgcaggctca ggcggtactc gctgcggctc gtcatcatgc ggtaggggtc catcacgccc
    15601 ttggtgacca ggtcgtccac cagcgtgccc aggtagctcg tgtgccgcgc cagcacaagt
    15661 tcttctttgc ccagcgcgtg gcgggcggcg tttaagccgg ccagcagacc ctgggcggcg
    15721 gcttcttcgt aaccggaggt gccgttgaac tgccccgcgc cgtagatgcc actgacgacc
    15781 ttgctttcca gcgtaggctt cagggtggtg gggtctacgc agtcatactc gatggcgtag
    15841 gccgggcgca tgatctcgag attttcaaac ccggcgatcg tgcggtacat ctcgttctgc
    15901 acggcctcgg gcaggctgga cgaaaggccc tgcaggtaca tttcctcggt gtcctcgccg
    15961 cagggctcca caaaaaccgg gtggcgctcc ttctctttga aacggacgac cttatcctca
    16021 atcgaagggc agtagcgcgg gccgacgccc tgaatgtcac cgccgtagag cggcgagcgg
    16081 tgcaggttgt cgagaatgac cttgtgggtc gcggggtttg tgtaggcgat gtagcagttg
    16141 accttgttgt gcatcggcac gcgggtcata aagctgaagg gctgcaattc gtcgtctggg
    16201 tcgccgggct ggcattcgag cttcgagaag tcgatgctgc ggcggtggac gcgggcgggc
    16261 gtgccggtct tgaagcggcg cagcgtgaag ccgtgcgcca ccagacattt tgtcagctcg
    16321 gtggcggcat gggtgccatc gggaccgctg tcatagtggg catcgccgac gaaaatgcgg
    16381 ccgcccaggt tggtgccggt ggccacgacg acggccttgc agccgtagta gccgtgcagg
    16441 ttggtgtaca caccgcagac cttgccgtcc tgtacatcta cgccgacgat ctcaccctgg
    16501 tggatatcga ggttggcggt cttttccagc gcgtgtttca tccagatatg gtagcgcttg
    16561 cggtcggtct gcacgcgcag gctgtgtacg gcagggcctt tgccgcggtt cagcatccgg
    16621 ctttgcaggt aggtggcgtc cgcggcaatg cccatcagcc cgcccagggc atcgacctcg
    16681 cggacgagat ggcccttggc cgtgccgccg atggacggat tgcagggcat attgccgata
    16741 aaatccagtg acagcgtaaa caccgcagtc cttgccccca gtttcgctgc cgcgtgcgcc
    16801 gcttcaatac cggcgtgccc cgcgccgatg acgatgacgt cgtaggttcc tagtttgtcc
    16861 atatactttc cttcttaccc tatcttttgc agaatatcct cgcagactcc tgcgaaattt
    16921 gtatccattt cattgttgcc atagcgcagc acgcggcagc caatgctttg cagatacgcc
    16981 gtgcgatgtg cgtcatactc ggcagcctgc ggttcggtat gctgtgcgcc atccagctct
    17041 attaccagtt tacacgatgg aatataaaaa tcaactataa aattgccaat catttttttg
    17101 acggtggacc gtttgcggga gatttttcag aaagtcatat caaagatgcc gttcctgcca
    17161 ggtcatttct ttgcggaggg ttcgggcatt tttgcgtaac ggatttatgg ttttgatgcc
    17221 tttcatagtt tgcctccact ttctaaaggc ttctcccttt gggagaagct gccgcccgca
    17281 ggcggctgat gaggggcggc tttaactcta ttgtcccttt accgagttac cgc
//
LOCUS       DBMDSQ010000011        17233 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_1058_length_17233_cov_2.105709, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000011 DBMDSQ010000000
VERSION     DBMDSQ010000011.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 17233)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 17233)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17233
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1058_length_17233_cov_2.105709"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..977
                     /locus_tag="ACZPAX_00860"
     CDS             <1..977
                     /locus_tag="ACZPAX_00860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0009987 - cellular process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HIR1112477.1"
                     /translation="LVRENKELTEQCEEANSALRVKGRAHDQARQQVLADRDKVISDA
                     ETEAARIRQQAKDDADKIIKDTNLKAEAIDQLAREQAIAQARQMVQSATDETREIQNA
                     HRLRLQDLKSRITEMEKARGEMMDYLAKVIGDLQKAQDYASRTAPLIVHKEDLEEADA
                     EPQLDLSALKVDAAASALRDSEPEPDAPAQPAAGETTNRVVAPGMDDDEPRTNTVVTP
                     SPDYFDTEPKAQPGDPNEEVEIPGAIFSYPILRQQGEPILDEGAPPAPVPHKPVMPSF
                     TLADDDEDGGDAVPASAPAPMPAPAGPQTKRRRKAIMALRALRKKLGA"
     gene            1007..2728
                     /locus_tag="ACZPAX_00865"
     CDS             1007..2728
                     /locus_tag="ACZPAX_00865"
                     /inference="COORDINATES: protein motif:HMM:NF028007.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HIR1112478.1"
                     /translation="MRLHKLWCCLLAVLALAALCAFGAAADTTVGVTGAGTFKMEQAY
                     VNVPELDVYFYALNGDGDPYSSIQVQAAGPELTLGDRKLEVRSVGIANDPICYIVALD
                     NSSGISPADFYTMLGGVRKLIAVMGENDQLMLYTTAGAAECVLPATSDKDLMYKALAH
                     IQQAEGSMDTAQLVSAVYSEIQSDYQALAPRKAAMIVTDAGQVLSNMALFGTLASDVG
                     DQIGMAAYVYLMTEEPKFFETLAQAADGKLVLCEASTLGDELKKKQAYFATALEIRTE
                     VPESMYGERLETLTLAMPQLGSAIKNSQTVYMGYKLTKPQVTNVETLRRDKLRLTFNQ
                     PIHENADKIQLYEIRSKDIWNWRVQVKSVEIAEDGRTATLEIEPLYKGEYTVALNRVS
                     SKMSAANVSSNRQTATFKVVVWPRDKEFYFARFRVPLLLATVLLLTLFGSWYGVRRRD
                     RAAEKEAEAEHLLAGAGEPDALPRRWVTLFWSQRPSIAESRWAGMVESSLIIGSDAAQ
                     CDLCIPDRKLSPQHCALAAQGDSLLVQTLTDRAKVYVNGERIEGEHRLQNNDTLRIGK
                     TTIRLVL"
     gene            2804..3289
                     /locus_tag="ACZPAX_00870"
     CDS             2804..3289
                     /locus_tag="ACZPAX_00870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HIR1112479.1"
                     /translation="MKLSKCESGHFYDSDKYPECPYCNTELLKDHSIVHEGEAEQPAA
                     VDKAAPAGSVAGWLVVLDGVAKGRDLRLGIGRSFLGVDADGTPVTLSADAPLSARQAA
                     VVYDAEKNAFTLLPGSSQELCYLGEDALLEPQPLTGGEVLRLGGAALQFVPFCGADFH
                     W"
     gene            3302..4819
                     /locus_tag="ACZPAX_00875"
     CDS             3302..4819
                     /locus_tag="ACZPAX_00875"
                     /inference="COORDINATES: protein motif:HMM:NF012235.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HIR1112480.1"
                     /translation="MTSEIVILITELRNKQRITLPAGSYRFGRSAQCEYVLRRNSVGD
                     NQFTVSFENGQWVVTDDGSDYRTWFNNRYLDSGETSVLQSGDVIGLNTDGDADTQEIT
                     FRVEEITNVQGDGAAPIKEQTSDAVLREVDVRRKKRVLIGRGDDCDIKLVSDRVSRHH
                     CEILYKDGHYELHDLGSTNGTYVDGMRVTRTVLRNGAVINVPAQVFAFTGGMLHYHAH
                     QSGISIQLVNVYKTVKNANTGRPLNIVDGTSFQVEPNSFVVLVGGSGTGKSSLLTCIT
                     GTAPCTAGSVRFDGLDTRSNRNAFEAALGYVPQKDIMHDNLTVEQSLTYTAKLRIAHD
                     ATRAEIAAAVAHAIEAVDLQGREKTFISRLSGGQKKRVSIAMELLANPRLLILDEPTS
                     GLSPDLDRSMMELCRRLSHQNCTVLMVTHNMSNINLCDKIAFLGVGGVLCYYGAPEKL
                     NDYFDVEMTSDIFEKLRDPEQIELYREKYYTTPEFNRLLAACPEAAQEADKRCSQ"
     gene            4807..5640
                     /locus_tag="ACZPAX_00880"
     CDS             4807..5640
                     /locus_tag="ACZPAX_00880"
                     /inference="COORDINATES: protein motif:HMM:NF024110.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1112481.1"
                     /translation="MQPVKFLKFLRQLGVLIQRNLQLIWNDKLLLASLALQSPFMALV
                     VKLTADPNCFTSNLVNIGSRTALFILAAMATFMGTLNSYREICKEREIILREASVGVS
                     LQAVVLSKAAVLLLVEIAQAAILALGFVSIVHVPQNHLLLETDMEIFVTLLLTMFSSS
                     CVGLLISALFTNGESAILAVLVLMIGQVVFSNVMFTVTGAAATISNIIVCRWGMGALG
                     ASTDLNSRMAWLQAGLDGPMYDATVENLTHSWQMLGLISVVCLVAAWLVLQIAFDRKK
                     A"
     gene            complement(5813..6757)
                     /locus_tag="ACZPAX_00885"
     CDS             complement(5813..6757)
                     /locus_tag="ACZPAX_00885"
                     /inference="COORDINATES: protein motif:HMM:NF018271.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease"
                     /protein_id="HIR1112482.1"
                     /translation="MPPSEFVVKTVYWFFLYGCIGWGVEVVYAAIKEHKLVNRGFLCG
                     PICPIYGFGMVGLVYSVSLIPMPSSGSMSAVAIFFIGMILTTAIELVGGWALFKVYRI
                     RWWDYSNMKFNLGGYICPQFSLLWGLGSVLMIKVVHPLLARGSNPMPFKAMLILDIVL
                     FIFFVVDVIVSTAAAIGLNKYLREIDELRARLRVTSDKLTTVLGTSAMTADTILDEQK
                     LQLALAKLEGRENAAELRAEVTARVGELREKLTAADHDFIGAHRLLRAFPDMKSLNYA
                     DTLAATRAATQRLRELAAAAKAAAKETAANAAEKIKKA"
     gene            complement(6744..7478)
                     /gene="trmB"
                     /locus_tag="ACZPAX_00890"
     CDS             complement(6744..7478)
                     /gene="trmB"
                     /locus_tag="ACZPAX_00890"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006735376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HIR1112483.1"
                     /translation="MRMRFKPYARPELLACDFHVHEPLTHGGHWHELYARPDQPWHLE
                     LGCGKGGFLAQIAPAHPDINYLGIDITDKVLILAKRKVEAAYAERGIPADNVKIASLD
                     IERLGNAFTPDDRVSRIYINFCNPWSKNAGSNKHRLTHPRQLLQYRSLMDEGAEIWFK
                     TDDDDLFRDSLSYFPAAGFEITWQTFDLHKNEPDWNLRTEHEGMFSEQGIPIKALIAR
                     KGPDSAVTWVEPKALAKQQEGEDAAE"
     gene            complement(7493..8611)
                     /locus_tag="ACZPAX_00895"
     CDS             complement(7493..8611)
                     /locus_tag="ACZPAX_00895"
                     /inference="COORDINATES: protein motif:HMM:NF019585.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA deacylase
                     activity [Evidence IEA];
                     GO_function: GO:0002196 - Ser-tRNA(Ala) deacylase activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HIR1112484.1"
                     /translation="MQTFTAQVLACTPVQGGFSVVLDRTAFFPEGGGQPCDTGTLGAA
                     RVLAVHTDGATITHTTDAPLTVGGTVEGCIDWPARLDAMQQHTGEHILSGTLHRLFGA
                     ENVGFHIGTPYVRMDTSIPLTAAQLAQAEAEANAAVRADTPVRCWVPDPETLAHTEYR
                     SKKALDGDVRLVEAGGDCCACCGTHLARTGEVGLIKIISYAHYKSGMRLAVACGQRAY
                     DAVAGIWADTEAAGRLLSSPVGSLAPALERLQNGETTLKARLAALQNTLADAYAAAAE
                     PGQPAVLWVDGADGDGLRRVAMFITAKTGAACCTLAPGGQGLAYALASAPGGDLRETC
                     KALNAAYQGRGGGKPGFCQGSLASGSFEQVKDFLLNTL"
     gene            8800..10035
                     /locus_tag="ACZPAX_00900"
     CDS             8800..10035
                     /locus_tag="ACZPAX_00900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1112485.1"
                     /translation="MALALPALLLAGCSLADVQATVLPAENTATIETAPTAAPQSLTV
                     YASASLAPAARAYADAQGVALTETDDAGAADLLLVDHASGGSLLDVTSDTLLAAAAAR
                     ADITDNANTLPLGRSLYGYWASKDALTALLGDDAVTALQGATWDEWSDFVEILSAWLA
                     APKAAVVTLSGQDYTLPETKPDAVKATGVFAQPADRAAGYSAAILAADGTYTADALTG
                     PLNGVYSAVVLEWDNMADSEANAVFRRAKLTDLLAEYGSDACQGMVLVPFKCNFDESD
                     LTTEEYNLDGLLDYPVLADVGCIAINAGTSADGLKAAKGAALWLYSNGEGEDALTETL
                     GVITPWNTASDATALGAMQVEQAGIGILPGTALDAATADALTANELALQGSESRTKAE
                     RTAFVKAALAALGAGETAE"
     gene            10094..11614
                     /gene="malQ"
                     /locus_tag="ACZPAX_00905"
     CDS             10094..11614
                     /gene="malQ"
                     /locus_tag="ACZPAX_00905"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007048709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HIR1112486.1"
                     /translation="MEFKRCSGILMPISSLPGGYGIGSMGKPARDFVDFLTKAGQTIW
                     QILPVGPTGYADSPYQSCSAFAGNPYFIDLDLLAADGLLTKKDYANINWGGDAAKVDY
                     GTLYEKRFTVLRKAYHNFMRQRPVPGYDTPYPDDWYRFQFLSSDWLPDYCLYMAIKED
                     NGMVDWQQWPRALRVREPEAMARFRAEHAEEIEFWAFLQYEFDRQWRSLHAYAKSKGI
                     AIMGDIPIYVAADSADAWAGRELFETDAEGKPRRVAGCPPDYFAADGQLWGNPLYDWN
                     YHRSTGYAWWIRRIRHALSIYDILRIDHFRGFDTYWAIPAGETTARNGRWEQGPRMDL
                     FRALHNALGSLPIVAEDLGEMFDSVRQLLAESGFPGMKVLQFAFTGEDSVDLPHNYPR
                     NCVAYPGTHDNNTLSGWFGEELTPAQRKQVEEYFVLTKQEGIVRGMLRGVQASTAALA
                     IIPMADWLEEPAASRMNTPGMAQGNWQWRIDAKKLTPALAKEMRTMAVRYFRHTDK"
     gene            11768..12559
                     /locus_tag="ACZPAX_00910"
     CDS             11768..12559
                     /locus_tag="ACZPAX_00910"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HIR1112487.1"
                     /translation="MKQDYTIAELQAEMTALQQVFDNVTLADPRMGLLDPATLQPLNK
                     VAAMPTLDSTGRGMQVVKGNDGLETVLAQGINVEGTPCMLLMQMPLPRSLQAEGAEDS
                     FIRTLSQMQDDLRRDYVTGAYNAVYLNEQYRPIAERAAMSGTPVGVVMVRVNEYWQLR
                     ERESDSAANCCLNMAAGILQLAVGPDHDKAVLARLNDGFFAIVTIGTPAARMAQTVRE
                     AMNDSRREFNISLSRRGSFTVEIASAEWGETASWDMTLSLAQQRL"
     gene            12629..13060
                     /locus_tag="ACZPAX_00915"
     CDS             12629..13060
                     /locus_tag="ACZPAX_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1112488.1"
                     /translation="MQTDNEKMLQEIVQNTEMGKNTLDELMGLTHDQKLKDEMMRQKR
                     EYRRFNQAAHTALDAIGAEAHGQSAAARMSVSMGIRTRTMMDKSTRNLATMLAEGSGQ
                     GVLDCKRAEKDYPTASPGAMQLCHELGCFQSEAEQTWRGFV"
     gene            complement(13209..14249)
                     /locus_tag="ACZPAX_00920"
     CDS             complement(13209..14249)
                     /locus_tag="ACZPAX_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015565970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HIR1112489.1"
                     /translation="MENIRAFLKRKDVIISAHRYGIDAMGAMAQGLFASLLIGTIIKT
                     LGQQTGIAFLVTAGGYAAAVAGPAMAASIGYALHAPQLVLFSLIAVGGAANELGGAGG
                     PLAVYLIAIAAAEIGKLVSKETKVDILVTPAVTILVGVGLSALCAPAIGAAASAVGSV
                     IMWATELQPLLMGILVSVLVGMALTLPISSAAICAALGLTGLAGGAAVAGCCAQMVGF
                     AVMSYKENGIGGLVSQGLGTSMLQMPNILRNPRTWIPPTLAAAITGPIATCLFHLEMN
                     GPAVSSGMGTCGLVGQIGVYTGWVESGKVITAFDWAGMVLVCFILPAVLSVIFCEVLR
                     KKDWIKEGDLKL"
     gene            14392..15147
                     /locus_tag="ACZPAX_00925"
     CDS             14392..15147
                     /locus_tag="ACZPAX_00925"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012958.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR1112490.1"
                     /translation="MVETFTVHITPFGLDRNTFVYLPDGWQTSGKRYPVVYMFDGHNL
                     FFDSTATYGTCWGLKEYMDAHGEAIIVAPECNHEGNARLEEYSPYDFTWQENAIHGRG
                     KAYLDWMVNELKPLIDAKYPTLPDRGNTAIGGSSMGGLMSLYGVTAHNATFSRAACLS
                     PSVRFSFAEVKADIKKAKLAPGTRVYISWGEREGKGRHALAQYTSKALEVTNLLTAQG
                     ASVYPYCQLDGKHCEADWRRQLGRCFKFLFGWR"
     gene            15151..15546
                     /locus_tag="ACZPAX_00930"
     CDS             15151..15546
                     /locus_tag="ACZPAX_00930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1112491.1"
                     /translation="MAKSHKKKKRPAPPAPAAPAAKPAPAPDTPPRKTRLLSALCYLN
                     VLILIPACCKQRHDAFVKFHLNQGLVVLALATVCACVGFLPHCSELGLTLTLLVDALS
                     LVGLVQTLRGLQTPLPLIWRITKSFHPFT"
     gene            15563..16117
                     /locus_tag="ACZPAX_00935"
     CDS             15563..16117
                     /locus_tag="ACZPAX_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1 family glyoxalase III"
                     /protein_id="HIR1112492.1"
                     /translation="MSKAVIFFAPGLEECEGLLCVDILRRAGVEVTIAAVGGERIVKS
                     ARQVNVVADALAEELDYAAFDACILPGGIPGVPNLKADATVRKVCTDFAAQGKIVAAI
                     CAAPTALAAFGVLGGKKATVYPGMDADLTAAGASYTGLPITIDGNIITGEALGAAIPF
                     ALALARTLAGADAANKVKTAIVYQ"
     gene            complement(16274..17209)
                     /locus_tag="ACZPAX_00940"
     CDS             complement(16274..17209)
                     /locus_tag="ACZPAX_00940"
                     /inference="COORDINATES: protein motif:HMM:NF014427.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HIR1112493.1"
                     /translation="MKNTKSVHPHKLAAAAVILACMLGAGLTGCSTTTKRAAPQRTAA
                     SEAATPETAPTATPAPTAAATPAPTEAPIVPTSVTLEQEGGILNKGMTWTLHATVLPE
                     DAADKTLHWTSSDEGVATIDDNGIITALAPGQTTITAASTAGEASTTYNLVVQEVVKC
                     SYCGLTGHTTDNCTAARQAAIVQQQQQAAAQAAVEQAAAQPAEAAQPAAEQPAAAAPA
                     QDYAVTAKGDRIYPEGSPDIPEGGVVQRPDGSFYVKGPRNVIFGYEEGDPDPNMPAWT
                     GLCVFCGDDDHDVYHCPILDAQENPDAFLIEDWEK"
BASE COUNT         3364 a         5348 c         5459 g         3062 t
ORIGIN      
        1 accttgtgcg ggaaaataag gaactgaccg agcagtgcga ggaggcaaac tccgccctgc
       61 gggtcaaggg ccgtgcccat gaccaggccc gccagcaggt gttggccgac cgcgacaagg
      121 tcatctctga tgccgagacc gaggccgccc gcatccgtca gcaggccaag gatgacgctg
      181 acaagatcat caaggacacg aacctgaaag ccgaggccat cgaccagctg gcccgggaac
      241 aggccattgc ccaggcgcgc cagatggtgc agtccgccac cgatgaaacg cgggagatac
      301 agaacgccca ccgcctgcgg ctgcaagacc tgaagagccg catcaccgag atggagaaag
      361 cccgcggtga gatgatggac tacctggcaa aagtcattgg ggatctgcag aaggcccagg
      421 actacgccag ccgcaccgcg ccgctgatcg tccacaaaga ggatttggag gaggccgacg
      481 ccgagccgca gctcgatttg tcggccctca aggtggatgc ggcggcctcg gccctgcggg
      541 acagcgagcc ggaacccgat gccccggccc agcccgccgc tggcgagacg acgaaccgcg
      601 tggtggcccc cggcatggac gacgatgagc cgcgcacgaa caccgttgtg acgccgtcgc
      661 ccgactattt tgacaccgag ccgaaagccc agcccggcga ccccaacgag gaagttgaga
      721 tacccggcgc aattttctcc tatccgattt tgcgccagca gggcgagccg attctggacg
      781 agggtgcgcc ccccgcacca gtgccccaca agccggtcat gcccagcttt acgctggcag
      841 acgacgatga ggacgggggg gacgccgtgc ccgcctcggc cccggccccc atgcccgccc
      901 ccgccggccc ccagaccaaa cgccgccgca aggctatcat ggcgctgcgt gctttacgaa
      961 aaaagcttgg cgcgtaggcg ctgtcaatca gaaaagaggg atgcccgtga gactgcataa
     1021 actctggtgc tgcctgctgg ctgtgctggc cctggcggca ctgtgcgcct ttggcgcggc
     1081 ggcggatacc accgttggcg tgaccggcgc gggcaccttt aaaatggaac aggcctacgt
     1141 caacgtgccg gagctggatg tgtatttcta cgcactgaac ggcgacggcg acccctattc
     1201 ctccatccag gtgcaggccg ccgggccgga actgacgctg ggcgaccgca agctcgaggt
     1261 acggtcggta ggcattgcca atgacccaat ttgctatatc gtcgcgctgg ataacagcag
     1321 cggcatcagc ccagcagact tttacacaat gctgggcggc gtgcgcaagc tcatcgcggt
     1381 gatgggcgag aatgaccagc tgatgctgta cacgacggcg ggggcggccg aatgcgtgct
     1441 gcccgccaca tcggacaaag acctgatgta caaggcactg gcacatatcc agcaggctga
     1501 gggcagtatg gatacagcgc agcttgtctc ggccgtatac agtgagatac agagcgacta
     1561 ccaggcgctg gccccccgta aagcggccat gatcgtgacc gatgccgggc aggtgctgtc
     1621 caatatggcg ctgttcggca cgctggccag cgatgtcggc gaccaaatcg gcatggcggc
     1681 ctacgtctac ctgatgaccg aagaaccgaa gttttttgaa acattggcac aggcggcaga
     1741 cggtaagctg gtgctgtgtg aagcatcgac gctgggggat gaactgaaga aaaaacaggc
     1801 ctattttgcc acagcactgg aaatccgcac cgaggtcccc gagagtatgt acggcgagcg
     1861 attggaaacg ctgacgctgg ccatgcctca gttgggcagt gccatcaaga acagccagac
     1921 cgtttacatg ggctacaaac tcacaaaacc ccaggtcacg aacgtggaga cgctgcggcg
     1981 cgacaagctg cgcctgacct tcaaccagcc catccacgag aatgccgaca agatacaact
     2041 ttacgagata cgctccaagg atatctggaa ctggcgcgtg caggtcaaat cggtagaaat
     2101 tgccgaggac ggccgcaccg caacgctgga gatcgagccg ctgtacaagg gcgaatacac
     2161 cgtggcgctg aaccgggtct ccagcaagat gagcgccgcc aacgtcagca gcaaccggca
     2221 gacggcgacg tttaaagtgg tcgtctggcc gcgggataag gaattttatt ttgcgcggtt
     2281 ccgagtgccg ctgctgctgg ccaccgtgct gctgctgacg ctgttcggca gctggtacgg
     2341 cgtgcgccgc cgtgaccgcg ccgccgaaaa agaggccgag gccgaacacc tgctggccgg
     2401 ggcgggggag ccggacgccc tgccgcgccg ctgggtcacg ctgttctgga gccagcggcc
     2461 ctccatcgcc gagagccgct gggccggcat ggtggagagc agccttatca tcggcagcga
     2521 tgcagctcag tgcgatttgt gcatcccgga tcgcaagctc tcgccgcagc attgcgcgct
     2581 ggcggcccag ggagactcgc tgctggtgca gacgctgacc gaccgggcca aggtgtatgt
     2641 gaacggtgaa cgcattgagg gcgaacaccg cctgcaaaat aacgataccc tgcgcatcgg
     2701 caagacgacg atacggttag ttttgtaggg aggcttgctc cctccgcggg gcgtcgagag
     2761 cgccgccccc ctgcatcgaa aaaccacaca aaggagcctt cttatgaagc tatccaaatg
     2821 cgaaagcggc catttttacg acagcgacaa ataccccgaa tgcccctatt gcaacaccga
     2881 actgctgaaa gaccattcca tcgtccatga gggcgaggcc gagcagcccg ccgccgtgga
     2941 caaggcggcc ccggcggggt cggtggccgg ctggctggtg gtgctggacg gcgtggccaa
     3001 gggccgtgac ctgcggctgg gcatcggccg cagctttctg ggggtggacg ctgacggcac
     3061 gccagttacc ttaagcgccg acgcgccctt gagcgcccgc caggccgccg tggtctacga
     3121 cgccgagaag aacgccttca cgctgctgcc cggttcctcc caggaactct gctacctggg
     3181 cgaggatgcc ctgctggaac cccagccgct gaccggcggg gaagtgctgc gcctgggcgg
     3241 tgccgcgctg caatttgtgc cgttttgcgg tgcagacttc cattggtaag ggggctgcgc
     3301 catgacaagt gagatcgtca ttttaattac ggaactgcgc aacaaacagc gtatcacact
     3361 gcctgcgggc agctaccgct ttggccgcag cgcccagtgc gagtacgtgc tgcgccgcaa
     3421 cagtgtgggc gataaccagt tcaccgtctc cttcgagaac ggccagtggg tcgtcaccga
     3481 cgacggcagc gactaccgca cctggttcaa caatcgctat ttggactccg gcgagacgag
     3541 cgtcctgcag agcggcgatg tcatcggttt gaacaccgac ggcgacgccg acacgcagga
     3601 aatcaccttc cgcgtggaag aaatcaccaa cgtgcagggc gacggcgcgg ccccgataaa
     3661 agaacagacc tccgacgccg tcctgcggga ggtggacgtc cgccgcaaga agcgggtgct
     3721 catcggccgc ggcgatgact gcgacatcaa gctcgtcagc gaccgcgtct cccgccacca
     3781 ctgcgagatt ctgtacaaag acggccacta cgaactgcac gacctcggct cgaccaacgg
     3841 cacttatgtg gacggtatgc gtgtgacccg cacggtgctg cgcaacggcg cggtcatcaa
     3901 cgttcctgcg caggttttcg cctttaccgg cgggatgctg cactaccacg cgcaccagag
     3961 cggcattagt atccaacttg taaatgtgta caaaacggta aaaaatgcca acacaggccg
     4021 cccgctgaac atcgtggacg gaacgagctt tcaggtggag ccgaacagct ttgtggtgct
     4081 ggtgggcggg tccggcacgg gcaaatccag cctgctgacc tgcatcaccg gcacagcccc
     4141 ctgcacggcg ggcagcgtgc ggtttgatgg gttggacacc cgctccaacc gcaacgcctt
     4201 tgaggccgcg ctgggctatg tgccgcaaaa agacatcatg catgacaacc tgaccgtgga
     4261 gcaaagcctg acctacacgg ccaagctgcg cattgcccac gacgcgaccc gcgccgaaat
     4321 cgccgccgct gtggcccacg ccatcgaggc ggtcgatttg cagggccgcg agaaaacgtt
     4381 tatctcccgc ctgtccggcg gccagaaaaa gcgtgtctcg attgcgatgg agctgctggc
     4441 caacccgcgg ctgctgattc tggacgagcc gacgagcggc ctttcccctg atttggaccg
     4501 cagcatgatg gagttgtgcc gccgtttgag ccaccagaac tgcacagtgc tgatggtcac
     4561 acacaatatg tccaacatca acctatgtga caaaatcgca tttctgggcg tgggcggcgt
     4621 gctgtgctac tacggtgcgc ccgagaagct gaacgactat tttgatgtgg aaatgaccag
     4681 cgatatcttt gaaaagctgc gcgatccgga gcaaattgaa ctgtaccgcg aaaaatatta
     4741 cacgaccccc gagttcaacc gcctgctggc ggcctgcccg gaggcagcgc aggaggcgga
     4801 taaacgatgc agccagtaaa atttttgaag ttcctgcgcc agcttggggt gctgatccag
     4861 cgcaatctgc agctgatttg gaacgacaag ctgctgctgg caagcctggc gctgcaatcg
     4921 ccgtttatgg cgctggtcgt caagctgacg gcggacccca actgctttac ctcaaacctt
     4981 gtcaacatcg gcagccgcac ggcgctgttc attctggccg ccatggcgac gtttatgggc
     5041 acgctgaact cctaccgcga aatttgtaag gagcgggaga tcatcctgcg cgaagcctcg
     5101 gtcggcgtca gcctgcaggc cgttgttttg agcaaggcgg cggtgctgct gctggtggaa
     5161 attgcccagg cggccattct ggcgctgggg tttgtgtcca tcgtccatgt gccgcagaac
     5221 cacctgctgc tggaaaccga catggagatt tttgtcacgc tgctgctgac gatgttctcg
     5281 tccagctgcg ttggcctgct gatttcggcg ctgttcacca acggtgaaag cgcgatcctg
     5341 gctgtgctgg tgctgatgat tgggcaggtc gtgttcagca atgttatgtt cactgtgacc
     5401 ggtgcggcgg cgacgatttc caacatcatc gtctgccgct ggggcatggg tgcgttgggc
     5461 gcatccaccg acctgaacag ccgcatggcc tggctgcaag ccgggctgga cggcccgatg
     5521 tacgacgcga ctgttgaaaa cctgacccac tcctggcaga tgctggggtt gatttcggta
     5581 gtctgccttg tggcggcctg gctggtattg cagattgcgt tcgacaggaa gaaggcataa
     5641 gaggcggtgc gggcggaatc tctccgacct cgctttaaca agggaggctt taatggcccc
     5701 cttgtcaaag gggctgtccg cgaagcggac tgggggattc cggccgcttg ctttagtaca
     5761 acataaacca acgccccgag cttttaacag ctcggggcgt atttgttgaa aattatgcct
     5821 ttttgatttt ttccgcagca ttcgcggcgg tctctttggc ggcggccttt gcagcggcgg
     5881 ccagttcacg caggcgctgg gtggcggcgc gggtcgcggc cagcgtgtcg gcgtagttca
     5941 ggctcttcat atccgggaaa gcccgcagca gacggtgcgc accgataaag tcgtggtcgg
     6001 cggcggtcag cttctcccgc agttcgccca cgcgggcggt gacttcggcc cgcagctcgg
     6061 cagcgttctc gcggccctcc agtttggcaa gggccagctg caatttctgc tcgtccagaa
     6121 tcgtgtctgc ggtcatggca ctggtgccca gcacggtggt cagtttgtcg ctggtcacac
     6181 gcaggcgggc acgcagctca tcgatttccc gcaggtactt gttcaggccg atggcggcgg
     6241 cggtggaaac aatgacgtcc accacaaaga aaatgaacag cacaatgtcc aaaatcagca
     6301 tggccttgaa cggcatcggg ttggagccgc gggccagcag cgggtgtacg actttgatca
     6361 tcagcacgct gcccagcccc cacaaaagcg agaattgcgg gcagatgtag ccgcccaggt
     6421 tgaacttcat gttagagtag tcccaccagc ggatgcggta gactttgaac agtgcccagc
     6481 cgcccaccag ctcaatggcg gtggtcagaa tcatgccgat aaagaagatg gcaacggcgc
     6541 tcatgctgcc gctgcttggc atcgggatca ggctgacgct gtacaccagc cctaccatgc
     6601 cgaagccgta aatcgggcag atcggcccgc agaggaaccc gcggttgacg agcttgtgct
     6661 ctttgatggc ggcgtaaacg acctcgacgc cccagccgat gcagccgtac aggaaaaacc
     6721 agtagacggt cttaacgaca aattcactcg gcggcatctt cgccctcctg ctgtttggca
     6781 agagctttcg gctccaccca ggtcacggcg ctgtcggggc ctttgcgggc aatcagcgcc
     6841 ttgatgggga tgccctgttc gctgaacata ccctcgtgct cggtgcgcag attccagtcc
     6901 ggctcgtttt tgtgcaggtc aaaggtctgc caggtaattt caaacccggc ggcggggaag
     6961 taagagagac tgtcccggaa caggtcgtcg tcgtcggtct tgaaccaaat ctccgcgccc
     7021 tcatccatca ggctgcggta ctgcaaaagc tggcgcgggt gggtcaggcg gtgcttattg
     7081 ctgccggcgt tcttgctcca cgggttgcag aaattgatgt agatgcgcga aacacggtcg
     7141 tctggcgtga aggcattgcc caggcgctca atgtccaggc tggcaatttt cacgttgtcg
     7201 gcgggaatac cgcgctctgc ataggcggcc tcgaccttgc gcttggcgag gatcagcacc
     7261 ttgtcggtga tgtcgatgcc gaggtaattg atatccgggt gggccggggc gatctgggcc
     7321 aaaaatccgc ccttgccgca gcccagctcc aggtgccagg gctggtcggg ccgggcgtac
     7381 agctcgtgcc agtggccgcc gtgggtcagc ggctcgtgaa cgtggaaatc gcaggccagc
     7441 agctccggcc gggcataggg tttaaaacgc attcgcatgg tggggtgtct ccttacaatg
     7501 tattcaacaa aaaatctttc acttgttcaa aactgccgct cgccaggctg ccttggcaaa
     7561 agccaggttt gccgccgccg cggccctggt aggctgcgtt cagcgctttg caggtctcgc
     7621 gcagatcgcc gcccggggca gaggccagcg cataggcaag gccctgcccg ccgggggcca
     7681 aagtgcagca ggccgcgccg gtcttggctg tgatgaacat tgccacacgg cgcaggccgt
     7741 cgccgtcggc accgtccacc cacagcacgg cgggctggcc cggttccgct gcggcggcgt
     7801 aagcgtctgc cagcgtgttt tgcagcgcgg ccaggcgggc tttcagtgtc gtctcaccgt
     7861 tttgcagccg ttcaagtgcc ggagccaggg agccaacggg cgaacttagc agccgcccgg
     7921 cggcctcggt gtcggcccag atgcctgcaa cggcgtcata ggcgcgctgc ccgcaggcca
     7981 cggccagccg cataccggat ttgtagtggg catacgagat aatcttgatc agcccgacct
     8041 cgccggtgcg ggccaggtgc gtgccgcagc aggcgcagca gtcgccgccg gcctccacca
     8101 gccgcacgtc gccgtccagc gcttttttgc tgcggtactc ggtgtgggcc agcgtttccg
     8161 ggtcgggtac ccagcagcgc accggggtgt cggcacgcac agcggcgttg gcttcagcct
     8221 cggcctgggc cagctgcgcg gcggtgagcg gaatgctcgt gtccatccgc acatagggcg
     8281 tgccgatgtg gaacccgacg ttctcggccc cgaacaatcg gtgcagcgta ccgcttaaaa
     8341 tatgctcacc ggtgtgctgc tgcatcgcgt ccagccgtgc gggccagtcg atgcagccct
     8401 ccacggtgcc gcccacagtc agcggggcgt cggtggtgtg ggtgatggtg gcaccgtcgg
     8461 tatgcacggc cagcacacgc gctgcgccca acgtaccggt gtcgcagggc tggccgccgc
     8521 cctcggggaa gaaggccgtg cggtccagca cgaccgaaaa gccgccctgc accggggtac
     8581 aggccagcac ctgcgcggtg aacgtttgca cataggaatc tgtttcgtat aatttttgcg
     8641 tcacgatagg cagaaccttt caaaattttg cgattttttt tattataaca taaaacaggt
     8701 gtggaaaaaa gttatcttat cctatataat aaaggatagc ctgaaatttt acaaccattt
     8761 atgagggaaa actgcgtaat gcaaactagg aaaactgctg tggcgctggc attgcccgcg
     8821 ctgctgctgg cggggtgctc gctggccgac gtacaggcca ccgtgctgcc cgccgaaaat
     8881 accgcgacca ttgagacggc tcccacagcc gcgccgcaat cgctgactgt ctatgcgtcg
     8941 gcgtcgctgg ccccggcggc cagggcctac gccgacgccc agggggtagc gctgaccgag
     9001 acggacgacg ccggcgcggc cgacctgctg ctggtggatc acgcctccgg cggcagcctg
     9061 ctggatgtga ccagcgacac gctgttggcc gcagctgccg cccgggctga catcaccgac
     9121 aacgccaaca ccctgccgct gggccgcagc ctgtacggct actgggccag caaggacgcg
     9181 ctgaccgccc tgttgggcga tgacgccgtg acggcgctgc agggggcgac ctgggacgag
     9241 tggagcgact tcgtcgagat actctccgcc tggctggctg cgccgaaagc tgctgtggta
     9301 acgctctccg gccaggacta caccctgccc gagacaaagc ccgacgccgt gaaagccacc
     9361 ggcgtgtttg cccagcctgc cgaccgcgcc gccgggtaca gcgccgccat cctggctgcc
     9421 gacggcacct acactgccga cgccctgaca ggcccgctga acggtgtgta cagcgccgtg
     9481 gtgctggaat gggacaacat ggccgacagc gaggccaatg cggtgttccg ccgtgccaaa
     9541 ctgaccgacc tgctggccga gtacggcagc gacgcctgcc agggcatggt gctggtgccg
     9601 ttcaagtgca attttgacga gagcgatctg accaccgagg agtacaacct ggatggtctg
     9661 ctggattacc cggtgttggc tgacgttggc tgcattgcca tcaacgcagg taccagcgct
     9721 gacggcctga aagccgccaa gggtgccgcg ctgtggctct actccaacgg cgagggcgag
     9781 gacgccctga ctgagacgct gggggtcatc accccctgga acacggcgtc ggatgcaacg
     9841 gcgctggggg ccatgcaggt ggaacaggcg ggtataggca ttttgcccgg caccgcgctg
     9901 gacgccgcaa ctgccgacgc cctgaccgcc aatgaactgg ccctgcaagg cagcgagagc
     9961 cgcaccaaag ccgagcgcac agcgtttgta aaggcggcgc tggcagcgct gggggcaggg
    10021 gagacggcgg agtaataaac cccatgcctt ccccccaagg gggaagcctt tatcgaagga
    10081 aaagagggac atcatggaat ttaaacgttg cagtggtatt ttgatgccga tttccagcct
    10141 gcccggcggg tatggcattg gcagcatggg caaaccggct cgtgattttg tggactttct
    10201 cactaaggcg gggcaaacga tctggcagat tctgccggtc ggccccacgg gctacgccga
    10261 cagtccctac cagagctgct cggcctttgc gggcaacccc tattttatcg atctggatct
    10321 gctggccgcc gacggcctgc tgaccaaaaa agactacgcg aacatcaact ggggcggcga
    10381 tgcagccaag gtcgattacg gcaccctgta tgaaaagcgc tttaccgtgc tgcgcaaggc
    10441 ctaccacaac tttatgcggc agcgcccggt ccccggctat gacacccctt acccggacga
    10501 ttggtaccgt ttccagtttt tgagcagcga ttggctgccc gattactgtc tgtacatggc
    10561 gatcaaagag gacaacggca tggtggactg gcaacagtgg ccccgcgcct tgcgcgtccg
    10621 tgagccggaa gccatggccc ggttcagggc cgagcacgcc gaggaaatcg agttctgggc
    10681 atttttgcag tacgagttcg accgccagtg gcgcagcctg cacgcctatg caaaatccaa
    10741 gggcattgcc atcatgggcg atatccccat ctacgtcgca gcggattccg ccgacgcctg
    10801 ggcaggccgc gagctgttcg agactgacgc cgagggcaag ccccgccgcg tggcaggctg
    10861 cccgcctgac tactttgccg ccgacggcca gctgtggggc aacccgctct acgactggaa
    10921 ctaccaccgc agcaccggct atgcctggtg gatccgccgc atccgccatg cgctgtccat
    10981 ctatgacatc ctgcgcatcg accacttccg cgggttcgac acctactggg ccatcccggc
    11041 aggggagacg accgcccgca atggccgctg ggagcagggt ccccgcatgg atctgttccg
    11101 cgcactgcac aacgccctgg gcagcctgcc catcgtggcc gaggacctgg gcgagatgtt
    11161 cgactcggtc cgccagctgc tggcggagag tggcttcccc ggcatgaaag tattgcagtt
    11221 cgcctttacc ggcgaggaca gcgtggatct gccccacaac tacccgcgca actgcgtggc
    11281 ctaccccggc acccacgaca acaacaccct ctccggctgg ttcggcgagg aactgacccc
    11341 tgcccagcgc aagcaggtgg aggaatactt cgtcctgacc aagcaggagg gcattgtgcg
    11401 cggtatgctg cgcggcgtgc aggccagcac ggcggcgctg gcgattatcc cgatggccga
    11461 ctggctggag gaacccgctg ccagccgtat gaacaccccg ggcatggccc agggcaactg
    11521 gcagtggcgc atcgacgcca aaaagctgac ccccgcgctg gcaaaggaaa tgcgcaccat
    11581 ggcggtgcgg tacttccgtc ataccgataa ataatttgtg tagttcgcat ggtatttttc
    11641 gcctggccga aaaatctttg tgcaatgcaa ggctaggaaa attgccggca ttgtggtaaa
    11701 ctatataagt tacgagagag aagctgtata ggccacaatg ccgaaacaca cggcagaagg
    11761 aaacaaaatg aaacaggact acaccattgc cgaattgcag gcagaaatga ccgccctgca
    11821 gcaggttttt gacaatgtta cgctggcaga cccgcgcatg ggtctgttgg accccgccac
    11881 cctgcagccg ctgaacaagg tggccgccat gcctacgctg gacagcaccg gccgcggtat
    11941 gcaggtcgtg aagggtaatg acggcctgga aaccgtgctg gcccagggca tcaacgtgga
    12001 gggcaccccc tgtatgctgc tgatgcagat gccgctgccc cgcagcctgc aggccgaagg
    12061 agcggaggat tccttcatcc gcaccttgag ccagatgcag gacgacctgc gccgcgacta
    12121 cgtgaccggc gcgtacaacg cagtctacct gaacgagcag taccgcccca ttgccgagcg
    12181 tgctgccatg agcggcacgc cggtcggcgt cgtgatggtc cgcgtcaacg agtattggca
    12241 actgcgcgag cgtgaaagcg actccgccgc caactgctgc ctgaacatgg cggcgggtat
    12301 cttacagctg gctgtcggcc ctgaccacga caaggccgtg ctggcgcgcc tgaacgacgg
    12361 tttcttcgct atcgtcacca ttggcacgcc cgccgccagg atggcccaga ccgtgcgcga
    12421 ggcgatgaac gattcccgcc gggagttcaa catttcgctg tcccgccgcg gcagcttcac
    12481 ggtggagatc gcctccgccg agtggggcga gacggcgtcc tgggacatga ccctctcgtt
    12541 ggcacagcag cgcctgtaac tgaataaacg atcccccttg tgtacatact ggcattgacc
    12601 gccaaacgta cacaaggggg attttgttat gcagaccgat aatgaaaaaa tgctgcagga
    12661 aattgtccag aacaccgaga tgggcaagaa cacgctggat gagctgatgg gcctgaccca
    12721 cgaccagaaa ttgaaggatg agatgatgcg ccagaagcgg gagtaccggc gcttcaacca
    12781 ggcggcgcac acggcgctgg atgccatcgg ggccgaggcc cacggccaga gcgccgctgc
    12841 caggatgagc gtgagcatgg gcatccgcac ccggacgatg atggacaaat cgacccgcaa
    12901 cctggccaca atgctggccg agggcagcgg ccagggcgtg ctggactgca agcgcgccga
    12961 gaaagactac cccacggcca gccccggggc catgcagctc tgccatgaac tgggctgctt
    13021 ccaaagcgag gccgagcaga cctggcgcgg tttcgtgtaa aaagccttcc ccctcttggg
    13081 ggaaggtgga cgtaggccgg atgaggggcg ggcctgccgt tacaacccgt ttatgggcaa
    13141 taatggcaaa cccacccctc atcagtctgc ttcgcagaca gcttccccct cgggggaagc
    13201 ctctttactt acaatttcag atcgccctct ttgatccagt ccttcttccg cagcacctcg
    13261 cagaagatga cgctgagcac agcgggcaga atgaagcaga ccagcaccat gccggcccag
    13321 tcaaaggcgg tgatgacctt gccgctctcg acccagccgg tgtacacgcc gatctgaccg
    13381 accaggccac aggtgcccat gccgctggac acggccgggc cgttcatttc cagatggaac
    13441 aggcaggtag caatggggcc ggtgatggcc gctgccagcg tcggcgggat ccaggtgcgg
    13501 gggttgcgca ggatgttggg catctgcagc atgctggtgc ccaggccctg gctgaccagg
    13561 ccgccgatgc cgttttcttt atagctcatc acggcaaagc ccaccatctg ggcgcagcag
    13621 ccggctacgg ccgcaccgcc cgcaaggccg gtcaggccca gcgccgcgca gatggccgcg
    13681 ctggaaatcg gcagcgtcag cgccatgccg acgaggaccg agacgagaat gcccatcagc
    13741 agcggctgca attcggtcgc ccacatgatg acgctgccca cggcggacgc cgccgcgccg
    13801 atggccgggg cacacagtgc gctcagacct acgccgacca aaatcgtcac agccggggtg
    13861 acgaggatat cgaccttcgt ctccttgctg accagcttgc cgatctccgc cgccgcaatg
    13921 gcgatcaggt agacggccag cgggccgcct gcaccgccca gctcattggc ggcgccgccg
    13981 acggcaatca ggctgaacaa aaccagctgc ggcgcgtgca gcgcgtagcc gatggatgcg
    14041 gccatggccg ggcctgcgac cgccgccgca tagccgcccg ccgtgaccaa gaatgcaatg
    14101 ccggtctgct ggcccagcgt cttgatgatg gtgccgatca gcaggctggc gaacagcccc
    14161 tgcgccatcg cgcccatggc gtcgatgccg tagcggtgcg ccgagataat aacgtccttg
    14221 cgctttaaaa atgcgcggat attttccatt ttacacactc ctcttctttt gttaaaagta
    14281 gagtaacatt ttgccgcgca gaatacaacc caccccgcac aatttttgtt gaaaaagccg
    14341 ggcaaatgcg ctacaataat agaaaatttg tgtaaaaggg ggagataccc tatggtcgaa
    14401 accttcaccg tccatatcac acccttcggg ctggatcgca acaccttcgt ctacctgccc
    14461 gacggctggc agacctccgg caaacgctac ccggtcgtct atatgtttga tgggcataac
    14521 ctgttttttg attccacggc cacctacggc acctgctggg ggctgaaaga atacatggac
    14581 gcccacggcg aggccatcat cgtcgcgccc gagtgcaacc acgagggcaa cgcccggctg
    14641 gaagagtaca gcccctacga cttcacctgg caggaaaacg ccatccacgg ccggggcaag
    14701 gcgtatctgg actggatggt caacgagctg aaacccctca tcgacgcgaa ataccccacg
    14761 ctgcccgacc gcggcaacac ggctatcggc gggtcgagta tgggcgggtt gatgagcctg
    14821 tacggcgtca cggcccacaa cgcgacgttc agccgcgcgg cctgcctgtc accgtcggtg
    14881 cggttcagct ttgccgaagt caaggccgac atcaaaaagg caaagctggc ccccggcacg
    14941 cgggtctaca tcagctgggg cgagcgcgag ggcaagggcc gccacgcgct ggcccagtac
    15001 acgtccaaag cgctggaagt caccaacctg ctgactgcgc agggcgcgtc ggtctacccc
    15061 tactgccagc tggacggcaa acactgcgag gccgactggc ggcggcagct gggccgctgc
    15121 ttcaaattcc tgtttggatg gaggtaaccc gtggcaaaat cacacaaaaa gaaaaagcgc
    15181 cccgcgcccc ctgcgcccgc cgccccggcg gccaaacccg cccccgcgcc ggataccccg
    15241 ccgcgcaaga cccggctgct gtcggcgctg tgctatttaa atgtattgat cctgataccc
    15301 gcctgttgca aacagcggca cgatgccttt gtaaagttcc acctgaacca gggccttgtg
    15361 gtgctggcgc tggccacagt ctgcgcctgc gttggctttc tgccccattg cagcgagctg
    15421 ggcctgacgc tgacgctgct ggtagatgcc ctgtccctcg tcgggctggt gcagaccctg
    15481 cgcggcctgc aaaccccgct gccgctgatc tggcgcatca caaagtcgtt tcacccgttt
    15541 acttaaacaa ggagtgtgta ttatgtctaa agctgtcatc ttttttgccc cggggctgga
    15601 ggagtgcgag gggctgctct gcgttgatat cctgcgccgc gcgggggtcg aagtgaccat
    15661 cgcggcggtg ggcggggagc gcatcgtcaa gagcgcccgg caggtcaacg ttgtggcgga
    15721 cgccctggcc gaggaactgg actacgcggc gtttgacgcc tgcatcctgc ccggcggcat
    15781 ccccggcgtg ccgaacctga aagccgacgc cacggtgcgc aaggtctgta cagactttgc
    15841 cgcgcagggc aaaatcgtgg cggccatctg cgccgcgccc acggcgctgg cggcgtttgg
    15901 cgtgctgggc ggcaaaaaag ccacggtcta cccgggcatg gacgccgacc tgaccgccgc
    15961 cggggccagc tacaccggcc tgcccatcac catcgacgga aacatcatca ccggtgaagc
    16021 cctgggcgcg gcgatcccct ttgccctggc cctggcccgg acgctggccg gggccgatgc
    16081 ggcgaataag gtaaagaccg cgatcgtgta tcaataagaa aatggcttct ccctcgggag
    16141 aagctgtccg cgaagcggac tgatgagggg cggctttgcc atgattgccc atgactgggt
    16201 tgacgtggca agtccgcccc tcatccggcc ttcggccacc ttccccccag ggggaaggct
    16261 tataaaaaac gcattacttc tcccaatctt cgatcaagaa cgcgtccggg ttttcctggg
    16321 cgtccagaat ggggcagtgg tagacgtcgt ggtcgtcgtc gccgcagaac acgcacaggc
    16381 cggtccatgc tggcatattg gggtcggggt cgccttcctc gtagccaaag atcacattgc
    16441 gggggccttt tacatagaac gagccgtcgg ggcgctgcac cacgccgccc tcggggatgt
    16501 cggggctgcc ctcggggtag atgcggtcgc ccttggcggt cacggcgtag tcctgggcgg
    16561 gggctgctgc agcgggctgt tcggcggcag gctgggctgc ctccgccggc tgagcggcgg
    16621 cttgctcgac ggctgcctgg gcggcggcct gctgttgctg ctgcacaatg gcggcctgcc
    16681 tggcggcagt gcagttgtcg gtggtgtggc cggtcaggcc gcaatagctg cacttgacga
    16741 cctcctgcac gacgaggttg taggtggtgg acgcctcgcc cgccgtgctg gcggcggtga
    16801 tggtcgtctg gcccggggcc agcgccgtga taatgccgtt gtcgtcgatg gtggcaacgc
    16861 cctcgtcgct ggacgtccag tgcagggtct tgtcggcggc atcctcgggc aggacggtgg
    16921 cgtgcagcgt ccaggtcatg cctttgttca aaatgccgcc ctcctgttcc agcgtcacgc
    16981 tggtgggcac gatgggcgct tccgtcgggg ccggggtggc cgccgcggtg ggggcaggcg
    17041 tggcagtggg ggcggtttcc ggcgtggcgg cttcactggc ggcagtgcgc tgtggggccg
    17101 cccgcttggt ggtggtgctg cagccggtca gcccggcacc tagcatacag gcaaggatga
    17161 ccgccgcagc ggccagcttg tggggatgaa ccgatttcgt gtttttcata gatgcattct
    17221 cctttgtggt tcg
//
LOCUS       DBMDSQ010000012        17229 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_1059_length_17229_cov_2.269967, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000012 DBMDSQ010000000
VERSION     DBMDSQ010000012.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 17229)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 17229)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17229
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1059_length_17229_cov_2.269967"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(16..465)
                     /locus_tag="ACZPAX_00945"
     CDS             complement(16..465)
                     /locus_tag="ACZPAX_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005920660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NanQ anomerase/TabA/YiaL family protein"
                     /protein_id="HIR1112494.1"
                     /translation="MVVDSFDYIACYKGLHPNLDTAIEWLNSHTLDALENGKTIIDGD
                     KVFVNVMDADLRDAEGAAFEYHRRYADLQIDLTGGEYLGWASEGKDEGAFDETADCGF
                     KSAPDHCGITLGDGRFAIFFPGELHKPSCKTPDCTHVRKAVVKILMK"
     gene            complement(477..1406)
                     /locus_tag="ACZPAX_00950"
     CDS             complement(477..1406)
                     /locus_tag="ACZPAX_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005944592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HIR1112495.1"
                     /translation="MKDITKYRGIIPAFYACYAPDGSISTERVKALTRHLIAKGVKGV
                     YVGGSSGECIYQHPDERKAVLEAVMSEAKGKITVIAHVACNNTADSVELAAHAEKCGV
                     DAIASIPPIYFHLPNDAIAAYWNAMSAAAPNTEFVIYNIPQLAGTALTMPLLQTMLKN
                     PNVIAVKNSSMPTQDIQLFKDAGIQARGEDGFAVFNGPDEQFVSGRVMGADGGIGGTY
                     AVMPELFIRMNELIEKGDIPTARAIQYKADRIIYKMCEAKGNLYAVMKEILRRMYGLE
                     LGGVRAPLANLAPEDEAVVAEAQKMIEEAIAAL"
     gene            complement(1679..2647)
                     /locus_tag="ACZPAX_00955"
     CDS             complement(1679..2647)
                     /locus_tag="ACZPAX_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005920663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR1112496.1"
                     /translation="MEATKTAAPATEQTKKKKKKVSPYMVFSTIVIILLAIAFAFPLY
                     WIITGSFKTQVSINATTPEWWPSEWVATNYAKLFSRQKAPLWEFAVPFSSNFSKDGKD
                     IIWWSGPQVPAAIRWLINTVFMAVASMILTCITSAMAGYALAKKRFVGRSVLFTLIVC
                     AMALPKQVILIPLLREMSSLNLYNTIWAVIFPIVGWPFGVFLMKQFSEGIPGEMLEAA
                     RIDGASEATTFTRIALPIIKPGIGALAIFTFINSWNDYFMQLIMLSSTSKLTISLGIA
                     KLQAENSTDYGLIMAGAALAAVPIIIVFLAFQKYFTKGITMGAVKG"
     gene            complement(2664..3542)
                     /locus_tag="ACZPAX_00960"
     CDS             complement(2664..3542)
                     /locus_tag="ACZPAX_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006875291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR1112497.1"
                     /translation="MSRALVRQETIAGYLFLLPSLVFFIGFVIYPMVMCVFTSFFDST
                     MNRADVFVGFQNYINLFHDSVFLGALRNTLIIVIVSVPITCIFSLWVASAIVNMPPWA
                     TSLFRVIFYLPVVTGSVAVTVVWKWMFNNYYGIFNYLGKSMGILDQNINWLGDARYAL
                     GCIILILLTTSVGQPIVLYVSALGNVDQSLVEAAEVDGATKLQAFWKIKWPQIMPTTL
                     YILVITTINSFQCFALIQLLTSGGPNHSTDTIMYYIYYTAFKLYKYGYGNAMGVILAL
                     MIAVLSAVQFKVTQSD"
     gene            complement(3712..5067)
                     /locus_tag="ACZPAX_00965"
     CDS             complement(3712..5067)
                     /locus_tag="ACZPAX_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016323728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR1112498.1"
                     /translation="MKKQVSAILAAGMAASLLVGCGGGSTSTAPAESTSTAASTEAAA
                     PAESTEAGQAADITLWTYPIGKWGDSESVDALLADFNAQYPDIHVTVEYLDYTNGDDQ
                     VNTAIEGGSAPDLVMEGPERLVANWGAKGLMVDLSDLWTDDQKADISASVESACKDSS
                     GAYYEFPLCMTAHCMAINKTVFEAADAMQYVDAENHTWTTDNFLKAVQAVYDSGKTDV
                     GAIYCSGQGGDQGTRAIINNMYGGTFTDAAHTKYTADSAENVKAIETLHNQDGINFDA
                     SINGGEEITLFRNGTLQMAFCWNIAQQTNSDNSAAGTTNNGDDILFMAFPTESGDPAL
                     CGGIWGFGIFDNGDENKIAASKTFINFMANSDETAKAVKTSTYFSVKNSLSDLYADDE
                     VMNEYQKLMPYLGDYYQVTPGWAEARTAWWNMLQQVGEGADVTTAVTEFCTTANAAAA
                     G"
     gene            5614..6282
                     /locus_tag="ACZPAX_00970"
     CDS             5614..6282
                     /locus_tag="ACZPAX_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009323437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HIR1112499.1"
                     /translation="MTYENMTRRRVLCYGDSNTYGYDPVRDGRYGDDERYPMVLQNLL
                     GDGWSVVEEGLPGRTAVFDDAITEGMNGLALITPILMSHAPLDTVTIMLGTNDSKNRF
                     DCSSYLISLGIVRLVKKALHTECWRDNANPDVLVIVPPSITPEYDKLKFREEMGPGCH
                     ERCAGIAAQLKPMLEGMAHVRFLDANTLPGAGCSDVDGMHMTLEAHKVLAKALQKELT
                     GSTL"
     gene            6356..7201
                     /locus_tag="ACZPAX_00975"
     CDS             6356..7201
                     /locus_tag="ACZPAX_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007048642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HIR1112500.1"
                     /translation="MVEQSFWEMLRTKRDSLTKSGIIVADYLLQHAEDAQYLSISSLA
                     KACGVAEATIFRFCRSLGFDGYNEMKIALAKATATALPVTLKLEPGVDTQTLCTHAYN
                     TAIEALNGTRSALDPDAIDRAATLLQRARQVYCFGQGGSQVLANDIWARFSTITTKFH
                     TAGDSHMQAITASLMGPEDVVLFVSYSGATRDMMDTLHLAKENGAKVILITHYDDAPG
                     VALADVVLLCGAKESPLDSGSMPVKLAVLFVANVLVLRYTLDNQELANLSLTRTSHAL
                     ANKLL"
     gene            complement(7290..7514)
                     /locus_tag="ACZPAX_00980"
     CDS             complement(7290..7514)
                     /locus_tag="ACZPAX_00980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1112501.1"
                     /translation="MFTLFVILVFGWLFFKSVGLAFHLTWGLAKIVATLLFVVALPLL
                     VVFAVLGALLPLLVPVALVAAAYGILKNYA"
     gene            complement(7699..8208)
                     /locus_tag="ACZPAX_00985"
     CDS             complement(7699..8208)
                     /locus_tag="ACZPAX_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006573797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1112502.1"
                     /translation="MGRTSTKENKTSYQLIREELGLTREKASELLETIAPERIEKIEN
                     ERTVPRPDEVLTMAEKYKRPSLCNYYCSRQCPIGEQYVPQVPMRELSAIVLEMLASLN
                     AMNKEKERLIEIAADGHISNDEIDDFIHIRKELERISVTVETLQLWTERMLANGQIDA
                     EIYKEKMAE"
     gene            8457..9101
                     /locus_tag="ACZPAX_00990"
     CDS             8457..9101
                     /locus_tag="ACZPAX_00990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HIR1112503.1"
                     /translation="MNWIDKLERRCGGRGIPYLINGLMLGQLAAGLIILLFSWQFAYQ
                     IDLSRAYLAGGQVWRLVTFLFQPIWLGSALGVLNLVFYFWVGNALTRVWGDFRMTLYI
                     ALGMLGAWISCFLTGYASCEPIFMSLLLAYAWLWPNQTVLLMGIIPFKIKYLGWYEMA
                     WWAVSFFRGGFPTKVCLVLGLAGFWAFYGREVFDWCVDTVKSWKRRRDWENRNR"
     gene            9139..9462
                     /locus_tag="ACZPAX_00995"
     CDS             9139..9462
                     /locus_tag="ACZPAX_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HIR1112504.1"
                     /translation="MKLITAIVNKEDSKNVCNELLKNKFYVTRLATTGGFLMAGNMTL
                     LICTDDARVDDCIGIISKCCKQRTEIVPGTATLGLGIESTMPMEVTVGGATVMVTNVE
                     RFEKL"
     gene            9462..10388
                     /locus_tag="ACZPAX_01000"
     CDS             9462..10388
                     /locus_tag="ACZPAX_01000"
                     /inference="COORDINATES: protein motif:HMM:NF024575.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1112505.1"
                     /translation="MLERLAGNDALKSELGAALRGGRLPHAVLLVGEPGCGTGFAARC
                     LAADYLYPQGGPHAEAVLKKEDTECLVLQGEGASGQIPVKKVREAREAIQRSALSTDA
                     AGRVLFIYGAQNLNGTQGSAANALLKIIEEPPEGVLFLLTAPNAAVVLPTIRSRCAAY
                     TIAPVPAADCAARLRAERLPAKAADELAFLYEGHIGTALKSWNDQTTKAALGMAKTLC
                     GYAAQGDTYRALALLTKYERDKEGFAALLWQLDQLCSAVLRRPAYGQEQCGGLTPDDA
                     AKILRADAGARRSLNGNGNLRLNVAVLAGEIT"
     gene            10420..11259
                     /locus_tag="ACZPAX_01005"
     CDS             10420..11259
                     /locus_tag="ACZPAX_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009323023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PSP1 domain-containing protein"
                     /protein_id="HIR1112506.1"
                     /translation="MKVVSVKFKENGRAYYFDPGAFKIKEGDFVVVTTARGTECGEVV
                     RAAHEVPGFSREVKPVIRVADAVDVRRMRQNRADVQHAYEICEQRIAAHNLKMKLVDA
                     EYTLDRSKLVFYFTADNRVDFRELVKDLAAQFHTRIELRQIGVRDESKMLGGLGLCGQ
                     PFCCSRFLKNFQPVSIKMAKEQGLSLNPAKISGSCGRLMCCLAYEQKSYEYLNSITPQ
                     VGSIVRTPDGEGTVIETNVVAGTLKVRSNVEILAPRIYKRDECAYIRGGKRAPQAPDK
                     EGE"
     gene            11457..11624
                     /locus_tag="ACZPAX_01010"
     CDS             11457..11624
                     /locus_tag="ACZPAX_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005929040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HIR1112507.1"
                     /translation="MAHTVSSECVACGACVDTCPVGAITMEDKAVVDAASCVDCGACE
                     GVCPTGAIQAE"
     gene            complement(11762..12505)
                     /gene="aroD"
                     /locus_tag="ACZPAX_01015"
     CDS             complement(11762..12505)
                     /gene="aroD"
                     /locus_tag="ACZPAX_01015"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HIR1112508.1"
                     /translation="MLTLRGLTFDAAAPRIIVPLFARSLGALRPLAAAADADPAAEVV
                     ELRLDPLPEVDFSAALALVRSLTDKPLIVTIRTKAEGGELALMPDAYAAHCRALLAQG
                     GVDALDIEWKACGPHRDALRNAAHAAGAVALYSEHHFDGTPATDAMADTLAGMADAGA
                     DIAKLAVMPHTAADAARLLEATARAHAARPGVPLITMSMGAIGAVTRFCGGAFGSAAT
                     FGVTSGTTASAPGQPSAEVLKEKLFVSGQ"
     gene            12633..13505
                     /locus_tag="ACZPAX_01020"
     CDS             12633..13505
                     /locus_tag="ACZPAX_01020"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013862.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HIR1112509.1"
                     /translation="MSKITIPGNSILLLEGGSLRGLYTAGVLDTFMENDLYFPAVAGV
                     SAGSLNAMNYVSRQPGRSASINLRYRHDPRYFGPKAALRGGSVFGLQFMLHDVKKEVP
                     FDEEMFRNGGMRMIAVATNVATGKPAYFEKGKTDFDFNEAVRASASLPLASTPVMLDG
                     KPYLDGGCSCPIALNWALNQGFEKIVVITTRQKGFRKTMPSQRMVELYDDFYGDKPLF
                     LADMLTQEMRYNTLMDQLDELEESGRVHCIRPNDPITIGRFEGDENKLLDLYNRGHRE
                     GREKLEELRTYLEK"
     gene            13715..14371
                     /locus_tag="ACZPAX_01025"
     CDS             13715..14371
                     /locus_tag="ACZPAX_01025"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR1112510.1"
                     /translation="MVKGVIFDMDGLMFDTERLWDKMWEPACKALGLPLPENLAAFSA
                     SCRGLAGDNLRSQVAKYIPGDPQRLLDKVWQMADERFAQGVPCKKGLKELLTALDDRG
                     MPRIVASSSPRTMIEMNLQNTGTARYFHDVVSGTEVKHSKPAPDTFLLAAEKLHLDPK
                     DCLVLEDSFNGVRAGRAAGCVTVMVPDMMQPTEEIMQLYDRKCEDLLEVRDLLVEGKL
                     "
     gene            14518..15129
                     /locus_tag="ACZPAX_01030"
     CDS             14518..15129
                     /locus_tag="ACZPAX_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005921554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HIR1112511.1"
                     /translation="MKNRTNIRWLTQLALLVAILLVLNYTPLGYLQIGPLSASLLTVP
                     VAIGAMTMGPTAGAILGGVFGATSFIQAVEGKSAMGAALFQVSPFGTFVVCFVARVLV
                     GLCAGLIFAALRKAMPKNEKLCCFLGGLAAPVLNTVFFMGFLVLIFYNCEYVQNLANT
                     LGAANAFMFIVLLVGVQAIFEWAICCVVAGAVSLPVRKYLKMN"
     gene            complement(15234..15968)
                     /locus_tag="ACZPAX_01035"
     CDS             complement(15234..15968)
                     /locus_tag="ACZPAX_01035"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015537103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin--[biotin carboxyl-carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HIR1112512.1"
                     /translation="MNESVIYLPEVDSTNLWAKHNLDKFGPIGAVYTTHQTAGRGRLG
                     RDWLDAPRKGLYYTAVIKTDLVQPATLPLFASLAVCDALRERYHIDCQIKWPNDLLLS
                     GKKIVGILCEVGPDGHSIVVGIGINLAQPQEYFDAAGLPHGTSLALQGVSVDMDTDPE
                     WLAQYMTDFGFDRALYTYESEGFAPYRERYKACCINLGRRVTYDGGAGIATDVDADGR
                     LIVHDESGDTRVFTGEVSVKGIYGAV"
     gene            complement(15965..16450)
                     /locus_tag="ACZPAX_01040"
     CDS             complement(15965..16450)
                     /locus_tag="ACZPAX_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005937569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HIR1112513.1"
                     /translation="MCKILVVSDVHGRLRDLRWLLNNETADAMFYLGDGLYDLNAALE
                     LRKEPVPYPIYRVAGNCDMNYNEPSEGMVPFGGVLFFYTHGHHYGVKMGSERLAECAG
                     ERGADVALFGHTHRRELVRGVGTAATVFNPGSLRDGGSYGVVTITNGKCSFTWKRVPE
                     F"
     gene            complement(16619..>17229)
                     /gene="rsmI"
                     /locus_tag="ACZPAX_01045"
     CDS             complement(16619..>17229)
                     /gene="rsmI"
                     /locus_tag="ACZPAX_01045"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009323020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HIR1112514.1"
                     /translation="NCALCSDAGMPCISDPGEQIVREAHERGIKVVPIPAASACVTAL
                     AASGQPTARFVFEGFLPVPKRDRRERLAFLQNETRTMIFYEAPHKLRGTLDDLAAAFG
                     AGRSVSLCRELTKLHEEIKKTTLGEAVAYYKENDPRGEYVLVLAGADPAAVEQAAAPT
                     LEEAVAAARALQANGLPPAAAAKQAAAGTPFSKGEIYKQLIQ"
BASE COUNT         3476 a         4751 c         5483 g         3519 t
ORIGIN      
        1 cttggggaat gcaaattatt tcatcaaaat tttgacgacg gcttttctta cgtgggtgca
       61 gtcgggcgtt ttgcagctgg gtttgtgcag ctcgccgggg aagaagatgg cgaaacggcc
      121 atcgcccagc gttatgccgc agtgatcggg ggcggacttg aaaccgcagt cggcggtctc
      181 gtcaaaggcg ccctcgtcct tgccctcgga tgcccagccc aggtactcgc cgccggtcag
      241 gtcgatttgc agatcggcgt agcggcggtg gtattcaaac gctgcgccct cggcgtcgcg
      301 caggtcggca tccatcacgt tgacaaaaac cttgtcgccg tcaatgatgg tcttgccgtt
      361 ttccagcgca tccagcgtgt ggctgttcag ccattcgatg gcggtgtcca ggttcgggtg
      421 caggcccttg taacaggcaa tgtaatcaaa cgaatctacg accatttttt taccccttac
      481 agtgctgcaa tggcttcttc gatcatcttc tgcgcttcgg cgacgacggc ctcatcctcg
      541 ggggcgaggt tggccagcgg ggcgcggacg ccgcccagct ccaggccgta catccggcgc
      601 agaatctcct tcatgaccgc gtacaggttg cccttggcct cgcacatctt gtagatgatg
      661 cgatcagcct tgtactggat ggcgcgggca gtggggatgt cgcccttctc gatcagctcg
      721 ttcatgcgga tgaacagttc gggcatgacg gcgtaggtgc cgccgatgcc gccgtcagcg
      781 cccatgacgc ggccggagac gaactgttcg tcgggaccgt tgaacacggc gaagccatcc
      841 tcgccgcggg cctggatacc ggcatccttg aacagctgga tatcctgggt gggcatggag
      901 ctgttcttga cggcgatgac gttggggttc ttgagcatgg tctgcagcag gggcatggtc
      961 agcgccgtgc cggccagctg cggaatgttg tagatgacga actcggtgtt gggggcggcg
     1021 gcgctcatgg cgttccagta ggcggcaatg gcgtcgttgg gcaggtggaa gtagatgggc
     1081 gggatggacg cgatggcgtc aacgccgcac ttctcggcat gggcggccag ctcgacggaa
     1141 tcggcggtat tgttgcaggc aacgtgggca atgacggtga tcttgccctt ggcctcgctc
     1201 atgacggcct caagaacggc tttgcgctcg tccggatgct ggtagatgca ctcgccggag
     1261 gaaccgccga cgtagacgcc cttgacgccc ttggcaatca ggtggcgggt cagcgccttg
     1321 acgcgctcgg tggagataga gccgtccggt gcgtagcagg catagaaggc ggggataata
     1381 ccgcggtact tggtaatgtc tttcataaac aaaacacttc ctgttcataa atagctgatg
     1441 ttacgttgaa ttttgaagga gaagggtggt gcggatatgt gggatgcggc gacacagcgt
     1501 gtcaaaaaat cacattcttg gtttatggaa tgtgtaggga cgaccatcgg tcgcccgcca
     1561 acttggcgca gcatcgcggt ttccgggtcg gttttcatac aaggcaaatg ggcacgggcg
     1621 aacaatgctc gcccctacaa gagcttttcg acaaccggaa cttccggttg tcggattttt
     1681 agcccttaac agcacccatc gtgatgccct tggtaaagta cttctggaag gcaaggaaca
     1741 cgatgatgat gggcacggcg gccagcgcgg cgccggccat gatcaggccg tagtcggtgg
     1801 agttctcggc ctgcagcttg gcgataccaa gggagatcgt cagcttgctg gtgctggaca
     1861 gcatgatcaa ctgcatgaag tagtcgttcc aggagttgat gaaggtgaag atggccagcg
     1921 caccgatgcc gggcttgatg atgggcagcg cgatgcgggt aaaggtcgtg gcttcgctgg
     1981 caccgtcgat gcgggcggct tccagcatct caccggggat gccctcgctg aactgcttca
     2041 tcaggaacac gccgaaaggc cagccgacga tggggaagat gaccgcccag atcgtgttgt
     2101 acaggttcag gctgctcatc tcgcgcagca gggggatcag gatgacctgc ttgggcagcg
     2161 ccatggcgca gacgatcagg gtgaacagaa cgctgcggcc gacgaagcgc tttttcgcca
     2221 gggcataacc ggccatggcg gaggtgatgc aggtcaggat catcgaggca acggccataa
     2281 agactgtgtt gatgagccag cggatggcgg cgggtacctg ggggccggac caccagatga
     2341 tgtccttgcc gtctttgctg aagttgctgc tgaacggcac tgcaaactcc cacagggggg
     2401 ctttctggcg gctgaacagc ttggcgtagt tcgtggcgac ccactcactg ggccaccatt
     2461 cgggcgtggt ggcgttgatg gacacctgcg tcttgaagga gccggtgatg atccagtaca
     2521 gcgggaaggc aaaggcaatc gccagcagga tgatgacgat ggtgctgaac accatgtagg
     2581 gggaaacttt cttctttttc tttttggtct gctcagtggc aggcgcagcg gttttcgttg
     2641 cttccatgtc aaatttcctc ctttcagtca gactgcgtga ccttgaactg cacagcggac
     2701 agcacggcaa tcatcagggc caggataacg cccatcgcgt tgccgtagcc gtacttgtac
     2761 agcttgaagg cggtgtagta gatgtagtac atgatggtat cggtggagtg attcgggccg
     2821 ccggaagtca acagctggat cagcgcgaag cactggaagg agttgatggt ggtgatgacc
     2881 aggatgtaca gtgtggtggg catgatctgc ggccacttga tcttccagaa ggcctgcagc
     2941 ttggtggcac cgtccacctc ggcagcctcg accaaagact gatcgacgtt gcccagggcg
     3001 gaaacgtaca gaacgatggg ctggccgacg gaggtggtga gcaggatcag gatgatgcag
     3061 cccagggcgt atcgggcgtc gcccagccag tttatgttct ggtcaaggat gcccatggat
     3121 ttgcccaggt agttgaagat gccgtagtag ttgttgaaca tccacttcca cacgacggtg
     3181 acggcgacgg agccggtgac gacgggcaga tagaagatga cgcggaacag cgaggtcgcc
     3241 cacggcggca tattgacgat ggcggatgcc acccacagcg agaagatgca ggtgatgggt
     3301 accgacacga tcacgatgat gagcgtgttg cgcaaggcac ccaggaacac gctgtcgtgg
     3361 aacaggttga tgtagttctg gaagccgaca aagacgtcgg cacggttcat ggtggaatca
     3421 aagaagctgg tgaatacgca catcaccatg gggtagatga cgaagccgat aaagaacacc
     3481 agactgggca gcaggaacag ataaccggcg atggtctcct gccggacaag cgcacggctc
     3541 atcttaggct cgctcttttt ggcggctgtg tttgccaaga ggatcgctcc cttctaaatt
     3601 taaaattcac aggtgagttt caataaaagc ggcgtcccac cgctgcgttt gaggtcagag
     3661 gtcggacgcc gtgggggtgt tacaaaggaa gaaggaggtt ttcagccgtt atcagccggc
     3721 agcggctgcg tttgcggtgg tgcagaactc ggtaacggcg gtggtcacgt cagcgccctc
     3781 gccaacctgc tgcagcatat tccaccaagc ggtacgagcc tcggcccagc cgggagtgac
     3841 ctgatagtag tcgcccaggt aaggcatgag cttctggtac tcgttcatga cttcatcgtc
     3901 ggcgtacagg tcagacaggg agttcttcac agagaagtaa gtagaggtct tgacggcctt
     3961 ggcggtctcg tcggagttgg ccatgaagtt gatgaaggtc ttggaagcgg cgatcttgtt
     4021 ctcgtcgccg ttgtcgaaga tgccgaagcc ccagatgccg ccgcacagag cgggatcgcc
     4081 ggactcagtg gggaacgcca tgaacaggat gtcgtcaccg ttgttggtgg tgccggccgc
     4141 gctgttgtcg ctgttggtct gctgggcaat gttccagcag aaagccatct gcagggtgcc
     4201 gttgcggaag agggtgatct cctcgccgcc gttgatggaa gcgtcgaagt tgatgccgtc
     4261 ctggttgtgc agggtctcaa tggccttgac gttctcagcg gagtcagcgg tgtacttggt
     4321 gtgggctgca tcggtgaagg tgccgccgta catattgttg atgatggcgc gggtgccctg
     4381 gtcgccgccc tggccgctgc agtagatcgc gccgacgtcg gtcttgccgg agtcgtagac
     4441 ggcctgcaca gccttcagga agttgtcggt ggtccaggtg tgattctcag catccacgta
     4501 ctgcatggcg tcggcagcct caaacacggt cttgttgatg gccatgcagt gggcggtcat
     4561 gcagagcggg aactcgtagt aagcaccgga ggaatccttg caggcggact caacgctggc
     4621 gctgatgtcg gccttctggt catcggtcca caggtcgctc aagtcgacca tcaggccctt
     4681 ggcaccccag ttggcgacca ggcgctcggg gccttccata acgaggtcgg gtgcgctgcc
     4741 gccctcgatg gcggtgttga cctggtcgtc gccgttggtg tagtcaaggt actcgaccgt
     4801 gacgtggatg tcgggatact gggcgttgaa gtcagccagc agtgcatcga cggactcgga
     4861 gtcgccccac ttgccgatgg ggtaggtcca cagggtaata tcagcggcct ggccggcctc
     4921 ggtggactct gcgggagcgg cggcctcggt ggaggcggcg gtggaagtgc tctcggcagg
     4981 ggcagtggag gtggaaccgc cgccgcagcc taccaacagg ctggcggcca taccggcggc
     5041 caaaatcgcg gatacttgct ttttcatacg ttgttctcct tttttgcctt tcggccttgt
     5101 tgggggcatc atcgttctgt gtccctcgtg atgtctctat tttagaaaac ttttttcatt
     5161 ttgtaaagat gtgaaatagc caagaaaaac ggccgtttct gtataagttg cacaaaatta
     5221 gctgcataca gtgcaaaatg cgcgcagaaa tacagcgatt tcgtgggttt gcggatgaaa
     5281 agtatttctt ttgcagttat tacaaaatga aaacaagttc caaaaatagg aggcttcctc
     5341 tgcgcaccgg cggaaaaaca gcaggcaaaa aaggcgatca ctcataagat ttatggaaaa
     5401 tcgcctttga ggcggccttg gaatactcct ttccctgcga aaatgaaaaa tgtcaaatga
     5461 ggcattcgtt tcgaggaact ttgcctattt tttgcacaaa tgcacaaaaa taggccgcaa
     5521 aaactggaag atagtcactt gcaacgaagg cattttccct atataatgaa ataaaaaaat
     5581 aacttttcat ttttaagaaa gggagcctct tttatgactt atgaaaacat gacccgccgc
     5641 cgcgtgcttt gctacggcga ttccaacacc tacggctatg acccggtcag agatggacgt
     5701 tacggcgacg acgagcgcta ccccatggtg ctgcaaaatc tgctgggcga cggctggtcg
     5761 gtggttgagg aaggcctgcc cggccgcacg gccgtgtttg acgatgccat caccgagggg
     5821 atgaacggcc tggcgctcat cacgccgatt ttgatgagcc acgcgccgct ggacaccgtg
     5881 accatcatgc tgggcacgaa cgacagcaag aaccggttcg actgcagctc ttatttgatc
     5941 tcgctgggca ttgtgcggct ggtgaaaaag gcgctgcaca ctgagtgctg gcgcgacaac
     6001 gccaacccgg atgtgctggt catcgtgccg ccgtccatca cgccggagta cgacaagctg
     6061 aaattccgcg aggaaatggg ccccggctgc cacgaacgct gcgcgggcat cgccgcccag
     6121 ctgaaaccga tgctcgaggg aatggcccat gtgcggttcc tggatgcgaa caccctgccc
     6181 ggggcgggat gctcggatgt ggatgggatg cacatgacgc tggaggccca caaagttctg
     6241 gccaaagcct tgcaaaagga actgacgggc agtacactat aaagataggg gcggagcatt
     6301 gccctgcggc agcaatctat tatcagaaaa atacaccgga aaggatccat ccaccatggt
     6361 tgagcagagt ttttgggaga tgctgcgcac caagcgcgac agcctgacaa aatcgggcat
     6421 catcgtcgcg gattacttat tgcagcacgc agaggacgca caatacctgt ccatctcgtc
     6481 gctggcgaag gcctgcggcg tggcggaggc tacgattttc cgattctgcc gctcgctggg
     6541 gtttgatggg tacaatgaaa tgaagatcgc gctggccaag gcgacggcca cggccctgcc
     6601 ggtcacgctg aagctggagc ccggcgtgga cacccagacc ctttgcaccc acgcctacaa
     6661 tacggccatc gaggcgctga acggcacccg cagcgccctg gaccccgacg ccatcgaccg
     6721 tgcggccact cttttgcagc gggcacggca ggtgtattgc ttcggccagg gcggcagcca
     6781 ggtgttggca aatgacatct gggcgcggtt ttccacgatt accaccaagt tccacacggc
     6841 gggggacagc catatgcagg caatcaccgc cagcctgatg gggccggaag atgtggtgct
     6901 gttcgtctcc tactccggcg caacgcggga catgatggac acactgcacc tggccaagga
     6961 gaacggcgca aaggtgattt tgataaccca ttacgacgac gcgcccggtg tggcgctggc
     7021 agacgtggtg ctgctgtgcg gtgccaagga aagcccgctg gactccggca gtatgccggt
     7081 caagctggcc gtgctgtttg tggcaaacgt gttggtgctg cgctacacgc tggacaacca
     7141 ggaactggcg aatctgtccc tgacaagaac gagccacgcg ctggcgaata agctgctgta
     7201 aaagaaagag ccttccccca agggagaagc catgcaaaca ataaccgccc ggcatcattg
     7261 cgatgccggg cggttccttt tttgtcaggt caggcgtaat tttttaaaat cccataggcg
     7321 gcggcgacaa gggcgacagg caccagcagg ggcagcaggg cgccaagcac cgcaaacacc
     7381 accagcagcg gcagggccac cacgaacagc agcgtcgcca caatttttgc cagtccccag
     7441 gtcaggtgaa acgccagccc caccgacttg aagaacagcc agccgaaaac caaaatcaca
     7501 aacagcgtaa acatcctcta tttctccttt tttataagta agcatttcag gtacagggtg
     7561 tcgctccatc accttgtact acgattttac cacgcgggtt attgcgcaaa cagggaaaat
     7621 ttagaaaaat agggggcgtt ttttactgtg gagagaagga aaagcgggtc gtcggggggg
     7681 ggcgacctct gcagctgatt actccgccat cttctcctta tagatctccg cgtcgatctg
     7741 accgttggca agcatccgct cggtccagag ctgcagcgtc tccacggtga cggagatacg
     7801 ctccaactct ttgcggatgt ggatgaaatc gtcgatctca tcattggaga tatgcccatc
     7861 agcggcaatt tcgatgaggc gttccttttc cttgttcatc gcattcagcg aggccagcat
     7921 ttccagcaca atggccgaaa gctcccgcat gggcacctgg ggcacatact gctccccgat
     7981 ggggcactgg cgggaacagt aatagttgca cagtgacggc cgtttgtact tttcggccat
     8041 cgtcagcacc tcgtcagggc gggggacagt gcgctcgttc tcgatttttt caatgcgctc
     8101 gggcgcaatg gtctccagca gctcactggc cttttcccgg gtcaggccca gttcctcgcg
     8161 gatcagctgg tagcttgtct tgttctcttt ggtggatgta cgtcccatat gcggctcctt
     8221 tttccttagg caacatcgca caattcccgc ctgacggctt aagcaccgct ccggcatctg
     8281 ccgcacctcg ttccccgtat cggcacttag aattatgcgg tattgcactt ataaaaataa
     8341 gcctagcata ttttacgccc cgccgcaagg gattcgacag ggaatttgtt gcactttggc
     8401 cgcagaattg ctataatata ttctgtaaat ctatatacac ggaggaactc ttccccatga
     8461 attggatcga taaactcgaa cgccgctgcg gcgggcgcgg catcccctac cttatcaacg
     8521 gcctgatgct gggccagctg gcggcggggc tgatcatcct gctgttcagc tggcagtttg
     8581 cctaccagat cgacctcagc cgcgcgtatc tcgcgggcgg gcaggtctgg cggctcgtca
     8641 cgtttctgtt tcagcccatc tggctgggca gtgcgctggg cgtgctgaac ctggtgttct
     8701 acttctgggt cggcaacgct ctgacccgcg tctggggcga cttccgcatg acgctctata
     8761 tcgccctggg tatgctgggc gcgtggatca gctgcttttt gaccgggtac gcttcctgcg
     8821 agccgatctt tatgagcctg ctgctggcct acgcttggct gtggcccaac cagaccgtgc
     8881 tgctgatggg cattatcccc ttcaaaatca agtatctggg ctggtatgag atggcctggt
     8941 gggcggtcag cttcttccgg ggcggcttcc ccacaaaagt gtgcctggtg ctggggctgg
     9001 ctggcttttg ggcgttctac ggccgtgagg tctttgactg gtgcgtggac accgtgaaaa
     9061 gctggaaacg ccgccgcgac tgggaaaacc gcaaccgctg aaatctgtaa tttttaacat
     9121 agaaaggtcg agtatactat gaaactcatc actgcgatcg tcaacaaaga ggattccaaa
     9181 aacgtctgta acgagctgtt gaaaaataag ttctacgtca cccgcctggc gaccaccggc
     9241 ggcttcctga tggcgggcaa catgacgctg ctcatctgca ccgacgacgc ccgcgttgac
     9301 gactgcatcg gtatcatctc caagtgctgc aagcagcgca ccgaaatcgt ccccggcacg
     9361 gccacgctgg gtttgggcat cgagagcacg atgccgatgg aagtcaccgt cggcggcgcg
     9421 accgtcatgg tcaccaacgt cgagcgtttt gaaaagctgt gatgctggaa cgtctggccg
     9481 gcaatgatgc gctgaaatcc gaactgggcg ctgccctgcg cggcggccgg ctgccccacg
     9541 cggtactgct ggtgggcgag ccgggctgcg gcacgggctt tgccgcgcgc tgcctggcgg
     9601 cggattatct ctacccgcag ggtggccccc acgccgaggc cgtgctgaaa aaagaggaca
     9661 ccgagtgcct ggtcttgcag ggcgagggtg ccagcgggca gatcccggtc aaaaaagtgc
     9721 gtgaggcccg cgaggctatc cagcggtcgg cgctttccac cgacgcggcg ggccgcgtgc
     9781 tgtttattta tggggcgcag aatttgaacg gaacccaggg ttctgcggcc aatgcacttt
     9841 tgaaaattat tgaggagcct ccggaaggtg tattgtttct tttgacagca cctaacgcgg
     9901 cggtggtgct gccgacgatt cgcagtcggt gcgcggcgta cacgattgcg ccggtgcctg
     9961 cggcggactg cgcggcccgg ctgcgcgctg agcgcttacc cgccaaggcg gcggacgagc
    10021 tggcctttct gtacgagggg catatcggca cggcgctgaa aagctggaac gaccagacga
    10081 cgaaagccgc gctgggcatg gcgaaaacgc tgtgcggcta cgctgcccag ggcgacacct
    10141 accgggcgct ggctctgctg acaaagtatg agcgcgacaa ggagggcttt gcggccctgc
    10201 tgtggcagct ggaccagctg tgcagcgccg tcctgcgccg cccggcctac ggccaggaac
    10261 agtgcggcgg cctgacaccg gatgacgcgg caaagatttt gcgcgccgac gccggggcac
    10321 gccggagcct gaacggcaac ggcaacctgc gcctgaatgt agccgtgctg gcgggggaga
    10381 taacgtaaaa aagacttcat tatataaaag gacaaaccaa tgaaagtagt atccgtcaaa
    10441 tttaaagaga acggccgggc gtattatttt gacccgggtg cgtttaagat caaggagggc
    10501 gactttgttg tcgtcaccac ggcccgcggt accgagtgcg gcgaggtcgt ccgcgccgcc
    10561 catgaggtgc cggggttctc ccgcgaggtc aagccggtca tccgcgtggc cgacgccgtg
    10621 gatgtgcgcc gtatgcgcca gaaccgcgcc gatgtgcagc acgcctacga aatctgcgag
    10681 cagcgtatcg ccgcccacaa cctgaaaatg aagttggtgg acgcagaata tacgctggat
    10741 cgcagcaaac tggtgttcta tttcaccgcc gacaaccgcg tggacttccg cgaattggtc
    10801 aaggatctgg ccgcgcagtt ccacacccgt atcgagctgc gccagattgg cgtgcgcgac
    10861 gagagcaaaa tgctgggcgg tttggggctg tgcggccagc cgttctgctg cagccgtttc
    10921 ctcaagaact tccagccggt gtccatcaag atggccaagg agcagggcct gagcctgaac
    10981 cccgcaaaaa tcagcgggtc atgcgggcgc ctgatgtgct gtttggctta tgaacaaaag
    11041 agttacgaat atctgaattc gattacgccg caggtgggca gcatcgtccg cacgccggac
    11101 ggcgagggca ctgtcatcga gacgaacgtt gtggcgggca cgctgaaagt acgttccaat
    11161 gttgaaattt tagctccgcg catctataag agggacgaat gcgcctacat ccggggcggc
    11221 aaacgcgccc cgcaggcccc ggataaagag ggcgaataat ggactataag tacggttagt
    11281 tgcggcttac cgtcgaaaaa tttcaagata tggcacttgc caaaatccaa acaatgtggt
    11341 acactaagta caacgcagta ggcagagcaa accgactgtg tgctgccccg gggagcagcc
    11401 gtgcagggcc gggacggtac aaaatataga ttcagcacgg agataaggag ttcacgatgg
    11461 ctcataccgt ttccagcgag tgcgttgctt gcggcgcttg tgttgacacc tgccccgttg
    11521 gtgcaatcac catggaggac aaggctgttg tcgatgcagc ttcctgcgtt gattgcggcg
    11581 cttgcgaggg cgtttgcccc accggtgcta tccaggctga gtaagctaca ttagcacaaa
    11641 tacagcgcgt tctctcaggc aaagagagaa cgcgcttttt gttttgtata gagcccgcgc
    11701 gtgtacggtg gacagccatt tgcgggaaag ttgcgggcgg catataggcc gcccctgcaa
    11761 atcattggcc ggagacaaat agtttttctt ttaaaacctc cgccgagggc tggccggggg
    11821 cgctggcggt ggtaccagag gtcacgccga aggtggcggc gctgccaaag gccccgccgc
    11881 aaaaccgtgt gacggccccg atcgcgccca tgctcatcgt gatgagcggc acgcccgggc
    11941 gggccgcgtg ggcgcgggct gtggcttcca gcaggcgggc agcgtcggcg gcggtgtggg
    12001 gcatcacggc cagcttggca atgtcggctc cggcgtcggc catacccgcc agcgtgtcgg
    12061 ccatggcgtc ggtggcgggc gtgccgtcaa aatggtgctc gctgtaaagt gccaccgcac
    12121 ccgccgcgtg ggcggcgttg cgcagggcgt cgcggtgggg gccgcaggcc ttccactcaa
    12181 tgtccagtgc gtccacgccg ccctgggcca gcagtgcgcg gcagtgggcg gcgtaggcgt
    12241 cgggcatcag ggccagctcc ccgccctcgg ctttcgtgcg gatcgtcacg atcagcggct
    12301 tgtccgtcag gctgcgcacc agcgccagcg cggcggaaaa atcgacctcg ggcagggggt
    12361 ccaggcgcag ttccaccacc tcggcagcgg ggtctgcgtc ggcggccgcg gccagcgggc
    12421 gcagggcacc caggctgcgg gcaaacagcg gcacaatgat acgcggtgcg gcggcgtcga
    12481 aagtcagacc gcgaagggtc agcataaaaa tcatcctctc ggggcagttt tttctatttt
    12541 agcacaattt ggccgaagtg taaaacaacc gcgctatttt gcttgcacaa cccctgtcaa
    12601 tcgggtaaaa tgctgaatat gaggtgagtt ttatgagcaa aatcacgatt ccgggcaact
    12661 ccatcctgct gttggagggt ggttcgctgc gcggtttgta caccgcgggc gtgctggata
    12721 ccttcatgga gaacgacctg tatttcccgg cggtggcggg cgtgtccgcc ggttcgctga
    12781 acgcaatgaa ctatgtctcc cgccagccgg ggcggtcggc cagtatcaac ctgcgctacc
    12841 gccacgaccc gcgctacttt ggccccaagg cggcgctgcg cggcggcagc gtgtttggcc
    12901 tgcaatttat gctgcacgac gtcaaaaaag aagtgccgtt cgacgaggag atgttccgga
    12961 acggtggtat gcggatgatt gcggtggcca cgaatgtagc gaccggcaag cccgcttatt
    13021 ttgagaaagg caagaccgat tttgacttca atgaggccgt gcgcgcgtcg gccagcctgc
    13081 cgctggcgtc cacgccggtc atgctggacg gcaagcccta cctggacggc ggctgctcct
    13141 gccccatcgc gctgaactgg gcgctgaacc agggctttga gaaaatcgtc gtcatcacca
    13201 cgcggcagaa ggggttccgt aagacgatgc ccagccagcg gatggtggag ctgtacgacg
    13261 atttttacgg cgataaaccg ttgttcttgg ccgatatgct gacccaggag atgcgctata
    13321 ataccctgat ggatcagctg gacgagttgg aagagagcgg ccgcgtacac tgcatccgtc
    13381 ccaatgaccc catcaccatc ggccgctttg agggcgacga aaacaagctg ctggacctct
    13441 acaaccgcgg ccaccgcgag gggcgggaaa agctggaaga attgcggacg tatttggaaa
    13501 agtgattttg ccttccccct tgggggaagg tggccgaagg ccggatgaag ggcgcgtttg
    13561 ccgatatgac ccagtaaggg tgcagtaagg caaggctgcc cctcatcaga ctgcttcgca
    13621 gtcagcttcc cccttggggg aagccttatt cttgcaatgg cggtcaaagt gcgttacaaa
    13681 aggtacaatt atgacataca cagaggagac accgatggtt aaaggcgtta tttttgatat
    13741 ggacggcctg atgtttgaca cggagcggct gtgggataaa atgtgggaac ctgcctgcaa
    13801 ggcgctgggg ctgccgctgc cggagaatct ggcggcgttc agcgccagct gccgcgggct
    13861 ggcgggggat aacctgcgca gccaggtggc aaaatacatc cccggcgacc cgcagcggct
    13921 gctggacaag gtctggcaga tggctgacga acggtttgcc cagggcgtgc cctgcaaaaa
    13981 gggcctgaaa gagctgctga ccgcgctgga tgataggggg atgccgcgca tcgtggcaag
    14041 ctccagcccg cgcacgatga tcgagatgaa tttgcagaac accggcacgg cgcggtattt
    14101 ccacgacgtt gtctccggca cggaggtcaa gcacagcaag cccgcgcccg acacgttttt
    14161 gctggcggcg gaaaagctgc acctcgaccc gaaggactgc ctggtgctgg aggacagctt
    14221 caacggtgtg cgtgcgggcc gcgcggcggg ctgcgtgacc gtgatggtgc cggacatgat
    14281 gcagccgacc gaggagatca tgcaactgta cgaccgcaaa tgtgaggacc tgctggaagt
    14341 cagagatttg ctggttgagg ggaagctgta ataagccttt tccccccgag gggggccaaa
    14401 aagcgcttgc cttttccttc tgtttgcggt atactgaatg tcggcggccc gggacgggcc
    14461 gtacatttgc gctgtactcc cgaattgtag gagacggcag caggaggaaa attgaatatg
    14521 aaaaaccgta ccaacatccg ttggctgacc cagcttgcac tgctggtggc cattctgctt
    14581 gtgctgaact atacgccgct gggctatctg cagatcggcc cgctgtcggc gtcgctgctg
    14641 acggtgccgg tggcaatcgg cgccatgacg atgggcccca cggcgggcgc catcctgggc
    14701 ggcgtgttcg gcgcgaccag ctttatccag gcggtcgagg gcaagagcgc catgggcgcg
    14761 gccctgtttc aggtcagccc gttcggcacc tttgtggtct gctttgtggc ccgcgtgctg
    14821 gtgggcctgt gcgccggcct gatttttgcc gccctgcgca aagcgatgcc gaagaacgag
    14881 aagctctgct gcttcctggg cggcctggct gcgccggtgc tgaacaccgt gttcttcatg
    14941 ggctttttgg tgctgatttt ttataactgc gagtacgtgc agaacctggc aaatacgctg
    15001 ggcgcagcca atgcgtttat gttcattgtg ctgcttgtgg gcgtgcaggc aatttttgag
    15061 tgggccatct gctgcgtcgt ggccggggcg gtttccctgc cggtgcggaa atatttgaag
    15121 atgaattaag aatttataat aaaaaggcga tggaacgtga aatgttccat cgccttttat
    15181 ttgtagagat gtaggggccg cgcatacccg gcccctacgg tgtggtgcca gtgtcacacc
    15241 gccccgtaaa tgccctttac gctgacttcg ccggtaaata cgcgggtgtc gccggattcg
    15301 tcgtggacga tgaggcggcc gtcggcgtcc acatcggtgg cgatgcctgc gccgccgtcg
    15361 taggtgacgc ggcggcccag gttgatgcag caggccttgt agcgctcacg gtagggggca
    15421 aagccctcgc tctcgtaggt gtacagtgcg cggtcaaagc cgaaatccgt catgtactgt
    15481 gccagccact cagggtcggt gtccatgtcc accgatacgc cctgcaaggc aaggctcgtg
    15541 ccgtggggca ggcccgcggc gtcgaaatac tcctgcggct gggccaggtt gatgccgatg
    15601 cccaccacaa tgctgtggcc gtcggggccg acctcgcaca aaatgccgac aatcttcttg
    15661 ccgctcagca gcaggtcgtt gggccatttg atctggcagt caatgtgata ccgctcccgc
    15721 agggcgtcgc agacggccag gctggcaaac agcggcaaag tggcgggctg gaccaggtca
    15781 gtcttgatga ccgccgtgta atacaggccc ttgcggggtg cgtcgagcca atcgcggccc
    15841 aggcggccgc gcccggcagt ctggtgggtg gtgtacaccg cgccgatggg gccgaacttg
    15901 tccaggttat gcttggccca caggttggta ctgtctactt ccggcagata gataacgctc
    15961 tcgttcaaaa ttcaggcacc ctcttccagg taaaactgca tttgccgttg gtgatggtaa
    16021 cgacgccgta gctgccgccg tcgcgcaggc tgccggggtt aaagacggtg gccgccgtgc
    16081 ccacgccgcg caccagctcg cgccggtggg tgtggccgaa caaggcgaca tccgcgccac
    16141 gctccccggc acactcggcc aggcgttcgc tgcccatctt gacgccgtaa tggtggccat
    16201 gggtgtagaa gaacagcacc ccaccgaaag gaaccattcc ctcgctgggt tcgttgtagt
    16261 tcatatcaca gttgcccgcc acgcggtaga tggggtaggg gactggctct ttgcgcagct
    16321 ccagcgcggc gttcagatcg tacagaccgt cgccgaggta gaacatagcg tcggcggtct
    16381 cgttattcaa aagccaacga aggtcgcgca ggcggccatg gacgtcggag acgactaaga
    16441 ttttacacat aggaaactcc tgttgaaaaa ggcttctccc ttgggagaag ctgtctgcga
    16501 agcagactga tgaggggcag gcttgccagg ctgcgctttt acggcgcagc gtgggcatac
    16561 gcgcccctca tccggcctcg cgggctcggc caccttcccc ccataggggg aaggcttttt
    16621 attggataag ctgcttataa atttcacctt tactaaacgg tgtcccggcg gcggcctgct
    16681 tggcggcggc agcggggggc aggccgtttg cctgcaaggc gcgggcagcg gcgacggctt
    16741 cttccaaagt cggcgcagcg gcctgctcaa cggcggcggg gtcggcaccg gccaggacca
    16801 gcacatactc gccgcggggg tcgttttctt tatagtaagc cacggcctca ccaagggtcg
    16861 tttttttgat ctcttcgtgc agcttggtca gctcgcggca gagcgagacg gagcgtcctg
    16921 cgccaaacgc cgcggccaaa tcgtccagcg tgccgcgcag cttgtgcggg gcctcgtaga
    16981 agatcatggt gcgggtctcg ttctgcaaaa aggcaaggcg ctcgcggcgg tcgcgcttgg
    17041 gcacaggcaa aaatccctcg aacacaaagc gggccgtggg ctggccgctg gctgccagtg
    17101 ccgtgacgca ggctgacgcg gccgggatgg gcacgacctt gatgccgcgc tcgtgggcct
    17161 cgcgaacgat ctgctcgccg gggtcgctga tgcagggcat accggcatca ctgcaaaggg
    17221 cgcagtttt
//
LOCUS       DBMDSQ010000013        16409 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_1124_length_16409_cov_2.129323, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000013 DBMDSQ010000000
VERSION     DBMDSQ010000013.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 16409)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 16409)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16409
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1124_length_16409_cov_2.129323"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="ACZPAX_01050"
     CDS             <1..231
                     /locus_tag="ACZPAX_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007488234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HIR1112515.1"
                     /translation="KEAFEFIDHLQIFSLLANVADVKSLVIHPATTTHAQLSPEELAD
                     VGIQENTIRLSIGTEHVDDIIADLDQAFAAVE"
     gene            480..1307
                     /locus_tag="ACZPAX_01055"
     CDS             480..1307
                     /locus_tag="ACZPAX_01055"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018214128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HIR1112516.1"
                     /translation="MMQIIEITDLSIPELAPYTKLTESQLRNKLEPEKGIFIAESPKV
                     IGTALDAGCEPLSFLMERRQIDGPAAGVLARCPDATVYTADRSVLQVLTGYVLTRGVL
                     CAMRRPAPHTAEELCRNAHRVAVLEGIVDSTNIGAIFRGAAALGMDAVLLSPSCCDPL
                     CRRAVRVSMGTVFQVPWAVLGDSPADWPEGGMARLHAMGFKTAAMALDNRAVSIDDPA
                     LHTEDKLAIVLGTEGDGLAHNTIAHCDYTVMIPMQHGVDSLNVAAAGAVAFWELRKR"
     gene            1494..2861
                     /locus_tag="ACZPAX_01060"
     CDS             1494..2861
                     /locus_tag="ACZPAX_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR1112517.1"
                     /translation="MGFLKKFTKQEISWMMYDWANSAHSVIVVTILPIFFDTVAGYTA
                     DSVSSMSTWGFATSLAMAIVALSAPFLGVFGDIRGMRKKLFLVFLLLGIASVGGLAFT
                     PFMDFTASPEAAGRVAAMVLVLYITSTIGFDASCIYYDAFLTDVTTEERMDSVSTLGY
                     GFGYIGGSTIPLLIFLIMNAVGVPMLTCLGFIFGLTAVWWLVFSLPLLKNCEQTTGKP
                     YKKGAVGAGIKSVFTTLKEIGADKPMLVYIVAYFFYIDGVHTIISMSTSYGTNLGLDS
                     AGMLLALLLVQVLGLPFCLLYMKLAEKFGARTMVGVGICVYMFICVFAFFMNSLWQFW
                     MLAVLCATSQGGIQALSRSMFGKLIPDKDRSGEFFGFFDIFGKFSSIMGPALVGFVSA
                     FAANKMLADQGLTAATATAEQIDAINKAAGPYGILSIILIIIVGAVLYFGVLPKTMTN
                     DERKI"
     gene            2999..3931
                     /locus_tag="ACZPAX_01065"
     CDS             2999..3931
                     /locus_tag="ACZPAX_01065"
                     /inference="COORDINATES: protein motif:HMM:NF013078.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HIR1112518.1"
                     /translation="MPEGYTHVRTAAKAAVAIHYKIQCPAAFAAGANGPDSFFCYEVW
                     KKRAKRHYDLPGLGNRMHEEKTGAFLRSLCANVKTRPQVEYTLGFLSHYATDTVVHPF
                     VCAMCEKGQPYGKKGGHGYLEIALDSTLHAEDTGSSLVPVEDVSPLPTGEELADITAL
                     LHTCLLETYGEDIPVEYLADAFWHTYKLRGIFPSKHGVRRAFFWLVEPLFGGRGFITG
                     HVSPRQLDKHLPDEWTDPFTGEHHDDGLFALLPRAVLRSEQFMGAALLYWMKKLTPAE
                     FAEKIGSMSYLQGIPTPVSDPDAQNTTTEKEISV"
     gene            3928..4398
                     /locus_tag="ACZPAX_01070"
     CDS             3928..4398
                     /locus_tag="ACZPAX_01070"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR1112519.1"
                     /translation="MIRITMQNGKTIDIELDHSAAPITCENFEKLVKDGFYNGLTFHR
                     VISGFMIQGGDPQGTGMGGPGWHIKGEFLQNGVANPIKHTRGVISMARSMDPNSAGSQ
                     FFIMHQDAPHLDGSYAAFGHVVKGIEVVDEIAAVATDWNDKPRTPVVMEKVEIV"
     gene            4573..5556
                     /locus_tag="ACZPAX_01075"
     CDS             4573..5556
                     /locus_tag="ACZPAX_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012451776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent 4,6-dehydratase LegB"
                     /protein_id="HIR1112520.1"
                     /translation="MAHTVMVTGADGFIGSHLTEELVKRGEKVRAFCLYNSFGSLGWI
                     DTLPPEIRNEIDVFMGDVRDPNGVRTAMRGQERVFHLAALIAIPFSYHSPDSYVDTNI
                     KGTLNVLNAARELGTQRVLVTSTSEVYGTAQYVPIDEKHPFQGQSPYSATKIGADRLA
                     ESFYRSFDLPVTIVRPFNTYGPRQSGRAIIPTIITQLLAGQTEIKLGSLTPTRDFNYV
                     KDTANGFMTIADCDAAIGQELNIATGVEHSIGDLANELIAQINPNAKIVCEAERLRPE
                     KSEVNRLLGDSTKLRELTGWAPQYTFEQGLAATIEFLRGNLDQYKVGQYIL"
     gene            5663..7345
                     /locus_tag="ACZPAX_01080"
     CDS             5663..7345
                     /locus_tag="ACZPAX_01080"
                     /inference="COORDINATES: protein motif:HMM:NF021436.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2142 domain-containing protein"
                     /protein_id="HIR1112521.1"
                     /translation="MSKVSKKKLLSPTVQHPAARWAFVLVLCAALGIVASYYWRVFYG
                     LDARGLAPLAVTGFCVGLAVLAGTAALLLRRVKDFAKRGAACILLCGILFAFANPPLQ
                     TPDETDHYLRTYAISMGHFDFDAERGYPEDVNELVAAFPGAWVNAHTSAGVGTDPDTH
                     AEQAYNTAGYALKQYGKDGRVESIWDSFTQYHNWESRDSAADSVTEPISFLILPFLPG
                     ALGMALARLLGFGALGCLYGGRLANLLAYAALCYAALRTAHKCKPAFLCIMLLPMSLY
                     MGASLSYDATLLGCYYVMLALLTRAEWDDRTALWYALACVFANGTKPYINLLWVVLPF
                     VVRRSEWKVRFSRALYAVLTAAGALALTFGVEQYGTLLRHNYGAIARQGGTTVNGGAQ
                     LLFVLKNPLRYIAVLLGTLYENDGFIGQLGLFGWKDMPVAFISLTAPLVLLAAALLCS
                     PSADALGRRRTGWLSAFGLVYAVGAMTAMYITYTPVGMVRIVGLQTRYFLPVWLLAVL
                     AAAALARRALAPRLTAEKAAVISVRLCGWYAFAGAVLLFQHYFVGPVYTIYK"
     gene            7392..9386
                     /locus_tag="ACZPAX_01085"
     CDS             7392..9386
                     /locus_tag="ACZPAX_01085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1112522.1"
                     /translation="MLHLLYALALLLLLSGACAILGEKSNLSPALLPLPVLSGAVVVL
                     YLCGIAGVLRAGAVLVLLALAAVWVLGLVQLRLAGVFKAWQSALYAPGFALFLGGAAF
                     IWVLFCVQKPMFTQWDEFTAWGLAPKMVVERGAFYVADPVNLKASFTYPATSLITFLF
                     QPFGHWAEWACLAAIDTLALTCLAAAAALPRERWACGVLVFAAGFLLPLFFSATPTGS
                     YATQYVNAMADLPLAMLFGGVFCLYYAVGREKRTFWLTALPLAVLTLTKDICFAYGLI
                     AAFLIGLDLLFAADGSVKKAFPKALLKAGGLAVVVLAAFLSWGRYTAAVTPTADTAAS
                     VGSEGLSYGAVLVGGVKQLLGIGRTEKFAQIMAAMGSAFFTRRICLLGGGAIAVAAIT
                     MVAAAAWLAAEKGPARRRVLAAHLGFAFCFAALYLFHLILYNYNFSDIEGLALKDYDR
                     YLAPYYQAWMLAMLCLLARSAKEQLGQLALGGAAAVIMAVFCWRGIPAAGFWTGANSL
                     YTLRADVQNRADTMNTVLNWPDRVLVISQGDDATRWYYYRYELTAQVVNGFGGFYGRL
                     GETQDRWDSDFMNLVESENWTLYDYKAVCVPDTLVAYMAEKDCDYILIDRADDYLQRE
                     FSPLFEGGLTNDMPATLYHFEGADADVPFTLAAVAESGVA"
     gene            9387..10010
                     /locus_tag="ACZPAX_01090"
     CDS             9387..10010
                     /locus_tag="ACZPAX_01090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1112523.1"
                     /translation="MKKPLFKARQLLVLAYLLAAVFWVVRYLVGCGVMLNYKMQGKMP
                     QTHVDAADLVTESFAPYSSNEWWTPPDDDPAWYLSTDSDPHIYWQGQGYIETVVLDAA
                     HRLPPGGVALYYLKPGQTDYTEAQKVYATVTAPGVYTFDLGGRWVTGLRIDPDSVGGV
                     PTLFTGIELNPARAWYTRFIPTAGWWLVLTFGPCIAAAFVSLFIRKK"
     gene            10176..12272
                     /locus_tag="ACZPAX_01095"
     CDS             10176..12272
                     /locus_tag="ACZPAX_01095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1112524.1"
                     /translation="MELLCVLAAVAALFGGCTVLTLKCRVPASVAPLTALSLIVAVLT
                     LAAMAGVLYPITWAVYALCLAGGVWVLATRKNHAGAAQKLFTPGSVLFWGMALAFTVY
                     FFVRQPMATDFDELSLWATAVKITKVNNDLYATAELGTPWAATQNPGLPLLAYFFQFL
                     GDYADWKIYVCYDILYFSVFAAVVGAIPRSKWRVAVPMAAALWCVPFFFTNYNHTIYL
                     TTTYMTSYGDVPAGLVFGGAVAAWLALRQENAPKWAVLPILALSANIKANTFVLALVA
                     AGLVAVDEWLFADDGDFKAGLLPRTGFSLACFAAPMAIYYLWNVRYVGWLVSRSASDS
                     GVGETSAPLSAVVVNGIKILLGQSVEGFYAEREAQFRTAMADMGHQFWTSDGKLSMIG
                     QGRNVVALIAIVFVVAILAAASRRLKARIAVIGVLSGVCFLGYNLMLALSYGFIFKDF
                     QAEQLTDYNRYIYSYYIGWFILALACLSVALLPQITVEGGADGPTAVFAATRRQPRLV
                     FELFVLALACGMLFRQSQLILPQLSVLGFANSEFTDRRAERAEAELVCSYLAPEDRVF
                     YVGQGDNGEGWFSAVFDFYPILVDYSGTVTTDPDTGETKMIGGGGELGLPELQPAEGV
                     KNTYYHAFTAEELDSIVRGNGCTVLYIQTLDDIFVQSYADLFTDKLAAAQSGETLLYR
                     VTDAGFAPMQMEVSAP"
     gene            12269..12904
                     /locus_tag="ACZPAX_01100"
     CDS             12269..12904
                     /locus_tag="ACZPAX_01100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1112525.1"
                     /translation="MKAMKNWFARRGALPITAYLLALAAWLVLGAAHFGSDALAKAQG
                     RLAEETMAVTDWQLVGLTQNEDGTLTTVDGDPQMILENVDGRVVRTISYTAQFDGEAR
                     EMCLYYTTKVGEDYSADRRVFPQSVGEGRYIYTLPRTSLAALRLDPCSPEENKTVTLA
                     VRDITLNAADTLPAVWQYFVPTWYQAFCLVLYPALAAAAVSMIFAVLKKEK"
     gene            13154..14662
                     /locus_tag="ACZPAX_01105"
     CDS             13154..14662
                     /locus_tag="ACZPAX_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009324856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HIR1112526.1"
                     /translation="MSEKEKILIADDSAMNRAILTEMLGDGYEILEAENGRQAVSIMQ
                     TNVDIDLLLLDIMMPEMDGFDVLAMMNKYHWIDDIPVIMISAENASSYVERAYDLGAT
                     DYISRPFDMAIVRRRVINTLMLYAKQKRLVRLVAEQVYEKEKSSSTMINILSHIVEFR
                     NGESGLHVLHIQTATDILLRTLVRKNDKYNLNAADISLISTASALHDIGKINIPASIL
                     NKPGKLTKEEFDTMKAHTTTGAEILDKLPFQQESPLVKTAYAICRWHHERWDGRGYPD
                     GLKGEDIPIAAQVVAMADVYDALTSERCYKKAFGHDKAMEMILNGECGQFNPLLLECL
                     TDAGARLHAELSLASSGGNRRNYLAEAQHISEVLLHEKSLPGQDHILRTLSVLRTKAN
                     FFAENARCVQFDYSEATGLVRFSPWAVEHMQVPEELHLPDEADRSGFAIADIDRIRTA
                     LRATTVNRPHAELSMLLPIDGELRWHHVSLCALWSDEIPPLYIGAVGQANPV"
     gene            14743..15087
                     /locus_tag="ACZPAX_01110"
     CDS             14743..15087
                     /locus_tag="ACZPAX_01110"
                     /inference="COORDINATES: protein motif:HMM:NF013767.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_function: GO:0043424 - protein histidine kinase binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HIR1112527.1"
                     /translation="MTLQEFYARVGGDYNATLSRLPSEALVKKFIMKYPGDPSFAQLK
                     DALAAQDWELAFRASHTLKGVAQNLGMDRLYKAAAALCDAVRGPKPLEDESLWPPVLA
                     AHEEVLAAIREL"
     gene            15308..>16409
                     /locus_tag="ACZPAX_01115"
     CDS             15308..>16409
                     /locus_tag="ACZPAX_01115"
                     /inference="COORDINATES: protein motif:HMM:NF012707.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HIR1112528.1"
                     /translation="MDEDGHRSGYSYEVLQRIAQHENVQFEYLAYDCDANEAADMLER
                     GEIDLLPTLRKTDERAERFDFSNEPISNVATMLTVKAGNRSIVAGNYDTFDGMVVGMS
                     RSGNGRNESFKAYAEKNGFTYTPVYFDTDEELSAALRSGTITAAVSNRMRQTSNEWVI
                     DTFDNEDVYIAVRKGDASTLRLVNDALIRMDRDDPTWRTTLFDKYNKNIHTSNKLYLT
                     AAEENYITAHNAADKEFTVLANPDRYPYSYLDKDGKLTGIMVDLFKLVAGRARLHYKF
                     LTPADRTEYKQILNDRAADFVIDLASDFSTAEDCGYKLTDSYLSAEFSWVLLRSHDGD
                     LRRVAVAYNFNTDVLELPGLDDCATVEYMDSFD"
BASE COUNT         3064 a         4993 c         4965 g         3387 t
ORIGIN      
        1 aaggaagcct ttgaattcat cgaccatctg cagatcttct ctctgctggc caacgttgcc
       61 gacgtgaaga gcctggtcat ccacccggcc accaccaccc acgcccagct ttcccccgag
      121 gagctggctg acgtcggcat ccaggagaac acgatccgcc tgtccatcgg caccgagcat
      181 gtcgatgata tcatcgctga tctcgatcag gcctttgccg ccgtggagta atccggcaaa
      241 gcatttttag agggtatata gggatttgaa tacgaccgcc gcccatggga tgcaagccct
      301 gcgggcggcg gttttgtttg caatttgctt taaaatattg tatgcttgtt ataaaaaccg
      361 cgaggcagag ttgtaagagc cgcacatgtg cggcccttga ccttgccttt actgcgcacc
      421 tgccttacac ggcagggccg ggtatacccg gtccctacac ctcaacgaag gaaaaagcca
      481 tcatgcagat tattgaaatt acagatttgt ccatccccga gcttgcccct tacaccaagc
      541 tgaccgaatc ccagctgcgc aacaagctgg aacccgaaaa gggtattttc atcgccgaaa
      601 gccccaaggt catcggtacg gcgttggatg ccgggtgcga gccgctctcg tttttgatgg
      661 agcgccgcca gattgacggc ccggctgccg gggtactggc ccgctgcccg gatgcgaccg
      721 tctacaccgc cgaccgcagc gtgttacagg tgctgaccgg ctatgtgctg acccggggcg
      781 tgctgtgcgc catgcgccgc cctgcgcccc acacggccga ggagctgtgc aggaacgccc
      841 accgcgtcgc cgttttggaa ggtattgtgg actctaccaa catcggcgcg attttccggg
      901 gcgcggcagc tctgggcatg gacgctgtgc tgttgtcgcc gtcctgctgc gacccgctgt
      961 gccgccgcgc cgtgcgtgtg agcatgggca cggtgttcca ggtgccgtgg gccgttttag
     1021 gcgacagccc tgcggactgg cccgagggcg gcatggcccg gctgcacgcc atgggcttta
     1081 agaccgccgc tatggcgctg gacaaccgcg ctgtcagcat cgacgacccg gcgctgcata
     1141 ccgaggacaa actcgccatt gtattgggca ccgagggtga cggcctggcc cacaacacga
     1201 ttgcccactg cgactacacc gtcatgatcc cgatgcagca cggagtagac tcgctgaatg
     1261 tggcggccgc cggagccgtg gcgttttggg agcttaggaa aagataattc cctaaggctt
     1321 cccccttggg ggaagccttc tcatcgtcaa actgcctcaa aaatatgacg aaagttttac
     1381 gccctgttga ccgaaacgtc aagggggcgt gttgttttta tgtttccaaa atgttacaat
     1441 atttatacaa gcgtgcgcaa tgcgtgcaat aacgaaacgg aggggtttct cccatgggct
     1501 tcctgaaaaa atttaccaag caggagatca gctggatgat gtacgactgg gccaacagcg
     1561 cccactcggt catcgtcgtg accatcctgc ccatcttctt tgacacagtc gcgggctaca
     1621 cggcggacag tgtttccagt atgtccacct ggggctttgc cacgagcctt gccatggcca
     1681 tcgtcgccct cagcgcaccg tttttgggcg tgttcggcga tatccgcgga atgcgcaaaa
     1741 agctgttcct tgtgttcctg ctgcttggca tcgcttcggt agggggattg gccttcaccc
     1801 cgtttatgga cttcaccgcc tcgcccgaag ctgcgggccg ggtcgcggcg atggttctgg
     1861 tgctctacat cacaagtacc atcggctttg atgcgtcctg catctactac gatgcgtttt
     1921 tgaccgacgt cacgaccgag gagcgtatgg actccgtttc caccttgggt tacggtttcg
     1981 gctacatcgg cggctcgacg attccgctgc tgattttcct catcatgaac gccgtcggcg
     2041 tgccgatgct gacctgcctt ggcttcatct tcggcctgac cgccgtgtgg tggctcgtgt
     2101 tcagcctccc gcttttgaaa aactgcgagc agaccaccgg caagccctac aagaagggcg
     2161 cggtcggcgc aggcatcaag agcgtgttta cgaccctgaa ggagatcggc gcggataagc
     2221 cgatgcttgt ctacattgta gcgtactttt tctacattga cggcgttcac accatcatca
     2281 gtatgtccac aagctacggc accaacctgg ggctggattc tgccggtatg ctgctggctc
     2341 tgctgctggt gcaggtgctg ggcctgccgt tctgcctttt gtacatgaag ctggcggaga
     2401 aattcggcgc gcgcacgatg gtgggcgtgg gcatctgcgt gtatatgttt atctgcgtgt
     2461 ttgcgttctt tatgaacagc ctgtggcagt tctggatgct ggccgttctg tgcgccacca
     2521 gccagggcgg catccaggcg ctgtcccgct cgatgtttgg caagctgatc cccgacaagg
     2581 accgcagcgg tgagtttttc ggcttctttg atattttcgg taagttctcg tccatcatgg
     2641 ggcctgcgct ggtggggttt gtcagcgcct ttgccgccaa caaaatgctg gctgaccagg
     2701 gcctgaccgc cgccacagcc accgccgagc agatcgacgc catcaacaag gctgccggcc
     2761 cgtatggtat tttgtccatc atcctgatta ttattgtcgg tgctgtgctg tatttcggcg
     2821 tgctgcccaa gacgatgacg aatgatgaga ggaaaatcta aaaaagataa aaaataagag
     2881 ataagagata aaagataata aaattggtgg gatgcgtcgc tgatgctccg caaatttgac
     2941 attttccccg ccccgcgtta caatattttc tacgctcacc aacgaaaggg gaatcccgat
     3001 gccggaaggc tatacccatg tgcgcaccgc cgcaaaagcg gcggtggcga tccattacaa
     3061 aatccagtgc ccggcggcgt tcgcggccgg tgccaacggc ccggacagct ttttctgcta
     3121 tgaggtctgg aaaaaacgcg caaaacgcca ctacgacctg ccgggcctcg gcaaccgtat
     3181 gcacgaggaa aagaccggcg cgttcctgcg cagcctgtgc gccaacgtca agacccgccc
     3241 ccaggtggag tatacgctgg gctttttgag ccactacgcc accgacaccg tcgtgcatcc
     3301 cttcgtctgc gccatgtgcg aaaaaggaca gccatacggc aaaaagggcg gccacggcta
     3361 tctggagatt gcactggact ccaccctgca cgctgaggat accggctcaa gccttgtgcc
     3421 cgtcgaggac gtcagtcccc tgcccaccgg cgaggagctg gccgacatta cggcgctgct
     3481 gcacacctgc ctgctggaaa cctacggtga agatatcccg gtggagtacc tggccgatgc
     3541 gttctggcac acctacaagc tgcgaggcat ctttcccagt aagcacggcg tgcgccgggc
     3601 gttcttctgg ctcgtggagc cgctgttcgg cgggcgtgga ttcatcaccg gccatgtatc
     3661 gccccggcag ctggacaaac acctgcccga cgagtggact gaccccttca ccggcgagca
     3721 ccacgacgac ggcctgtttg cgctgctacc ccgcgcggtt ttgcgcagcg aacagtttat
     3781 gggcgcggct ttgctctact ggatgaagaa gctgaccccc gccgaatttg ccgaaaagat
     3841 cggcagcatg agctatttgc agggcatccc caccccggtg agcgaccctg acgcacaaaa
     3901 taccacaact gaaaaggaga tttccgtatg atccgtatca ccatgcagaa cggcaagacc
     3961 attgacattg agctggacca tagcgccgcc cccatcacct gcgagaactt tgaaaagctc
     4021 gtcaaggacg gtttttacaa cggcctgacg ttccaccgcg ttatttccgg ctttatgatc
     4081 cagggcggcg acccccaggg caccggcatg ggcggcccgg gctggcacat caagggtgag
     4141 tttttgcaga acggcgttgc caaccccatc aagcacaccc gcggcgtcat cagcatggcc
     4201 cgctccatgg atccaaactc cgccggcagc cagttcttca tcatgcacca ggacgccccg
     4261 cacctggacg gcagctacgc tgctttcggc cacgtggtga agggcattga ggtcgtcgat
     4321 gagattgccg ccgtcgccac cgactggaac gacaagcccc gcaccccggt cgtgatggaa
     4381 aaggtcgaga tcgtctaatt taccacgcgg tagcacccgg atgttcgcgc atccgggtgt
     4441 tttgtttggg caatcgcgcg cctaatcgcg cgcctttaga ataatagttg aaatcttccc
     4501 aaaaatcggt tatactattt tgtaataaag ttgaaatatg gggcattgtc ccctttgata
     4561 aaggagcaca aaatggcaca taccgttatg gtcaccggtg cagacggctt tattggcagc
     4621 catctgactg aagaactcgt caagcgcggc gaaaaggtcc gcgcattctg cctgtacaat
     4681 tccttcggct cgctgggctg gatcgacacg ctgccgccgg agatccgcaa tgaaattgat
     4741 gtctttatgg gcgacgtgcg cgaccccaac ggcgtgcgta ccgccatgcg cgggcaggag
     4801 cgggtgttcc acctggcagc gcttatcgct atccctttct cctaccacag cccggacagc
     4861 tatgtggaca ccaacatcaa gggcacgctg aacgtgctga acgccgcccg tgagctgggc
     4921 acccagcgcg tgcttgtcac ctccaccagc gaggtctacg gcacggcgca gtatgtgccc
     4981 atcgacgaga agcacccctt ccagggccaa agcccctact ccgccaccaa aattggtgcg
     5041 gaccgcctgg ctgagagctt ctaccgcagc tttgacctgc ctgtcaccat cgtgcgcccc
     5101 ttcaacacct atggcccgcg ccagagcggc cgcgccatca tcccgacgat tatcacacag
     5161 ctgctggcag gccagaccga aatcaaactc ggcagcctga ccccgacccg cgacttcaac
     5221 tatgtcaagg acacggccaa cggctttatg acgattgccg actgcgacgc cgccatcggc
     5281 caggagctga acattgccac cggcgtggag cactccatcg gtgacctggc caacgagctg
     5341 attgcccaga tcaacccgaa tgcgaagatc gtctgcgagg ccgagcgcct gcgccccgaa
     5401 aagagcgaag tcaaccgcct gctgggcgat tccaccaagc tgcgcgagct gaccggctgg
     5461 gcaccgcagt acacctttga gcagggcctg gccgccacca tcgagttcct gcgcggcaac
     5521 ctggatcagt acaaggtcgg ccagtacatc ctgtaatgca ggggccgggt atacccggcc
     5581 ttcgaccttg ccgcaccggg cactttgcgg cgcggcaaac agccgggcga ggtatgcctc
     5641 gcccctacca aaatctaagt ttatgtcaaa agtttccaag aaaaagttac tttcacccac
     5701 cgtgcagcac cccgccgccc ggtgggcttt cgtgttggtg ctgtgcgccg cgctgggcat
     5761 cgttgccagc tactactggc gcgtattcta cgggctggac gcccggggcc ttgcgccgct
     5821 ggccgtaacc ggcttttgtg tagggttggc tgtgctggcg ggcacagcgg cactgctgct
     5881 gcgccgggtc aaagattttg ccaaacgggg cgccgcctgc attttgctgt gcggcatact
     5941 gtttgccttt gcaaacccgc cgctgcagac ccccgatgag acagaccatt atctgcgcac
     6001 ctatgcgatt tccatggggc actttgactt tgacgccgag cgcggctacc ccgaggacgt
     6061 gaacgagctg gtcgcggcgt ttcccggcgc atgggtcaac gcccatacct cggccggcgt
     6121 cggcacggac cccgataccc acgccgagca ggcctacaac acggcaggct atgcgctgaa
     6181 gcaatacggc aaggacggcc gggtcgagag catctgggac agctttacgc agtatcacaa
     6241 ctgggaaagc cgcgattctg cggcagattc cgtaactgag cccatcagct ttttgatttt
     6301 gccgtttttg cccggggcac tgggcatggc gctggcgcgg ctgctgggat ttggtgcgct
     6361 gggctgcctg tacggcgggc gactggccaa cctgctggcc tacgcggcgc tgtgctatgc
     6421 ggcgctgcgc actgcgcaca agtgcaagcc tgcttttttg tgtataatgc tgctgccaat
     6481 gtcgctctac atgggcgcat cgttaagtta tgacgccacg ctgctgggct gctactacgt
     6541 catgctggcg ctgctgaccc gcgccgagtg ggacgaccgc acggcgctgt ggtatgcgct
     6601 ggcctgcgta tttgccaacg gcaccaagcc ctacatcaac ctgctgtggg tcgtgctgcc
     6661 ctttgtggtg cgcagatccg agtggaaggt gcggttcagc cgcgccttgt acgccgtgct
     6721 gacagcggcg ggtgcgctgg cactgacctt tggcgttgag cagtacggca cacttttgcg
     6781 ccacaattac ggcgcgattg cccggcaggg cggcaccacc gtgaacggcg gtgcgcagct
     6841 tttgtttgtg ctgaagaatc ccttgcgtta tatcgcggtt ttgctgggta cactgtatga
     6901 gaacgacggt tttatcggcc aactgggctt gttcggctgg aaggatatgc cggtggcatt
     6961 tatcagcctg accgcgccgc tggtgctgct ggcggcggcg ctgctctgct cgccgtctgc
     7021 tgatgccctg ggccgccgcc gcaccggctg gctgtcggcg ttcggtctcg tctacgccgt
     7081 gggcgcgatg acggccatgt atattaccta cacccccgtg ggcatggtgc gcatcgtcgg
     7141 cctgcagacg cggtatttcc tgccggtctg gctgctggcg gtgctggctg ctgccgcgct
     7201 ggcccgccgt gcgcttgccc cgcgcttgac ggcagaaaag gccgccgtca tcagcgtccg
     7261 cctgtgcggg tggtatgcgt ttgcaggggc agtgctcttg ttccagcact actttgtggg
     7321 gccggtgtat acgatttata agtaaaaggg cttcccccaa gggggaaggc attccacgaa
     7381 aggaggctcc catgctgcat cttctctacg cccttgcgct tttgctttta ctgtccggcg
     7441 cttgtgcgat actgggcgaa aaatccaatc tctcccccgc cctgctgccg ctgccggtgt
     7501 tgagcggcgc ggtggtcgtg ctgtacctct gcggtatcgc gggcgtcctg cgggctgggg
     7561 cggtgctggt gctactggcg ctggccgccg tgtgggtgct gggccttgtg cagctgcgcc
     7621 tcgccggggt gttcaaagcg tggcagagcg ccctgtacgc gccgggcttt gcgctttttt
     7681 taggcggtgc tgcgtttatc tgggtgctgt tctgcgtaca gaagcccatg tttacccaat
     7741 gggacgagtt cacagcctgg ggcctggccc ccaagatggt cgtggagcgc ggcgcgttct
     7801 acgtggctga cccggtcaac ctgaaagcca gtttcaccta cccggccacg agcctgatta
     7861 cgttcttgtt ccagcctttc ggccactggg ccgagtgggc ctgcctggcc gccattgaca
     7921 cgctggcgct gacctgcctg gccgccgctg ccgccctgcc gcgggagcgc tgggcctgcg
     7981 gcgtgctggt gtttgccgct gggttcctgc tgccgctctt cttctcggcc acgcccaccg
     8041 gcagctacgc cacccagtac gtcaacgcca tggccgacct gccgctggca atgctgtttg
     8101 gcggcgtgtt ctgcctgtat tacgcggtgg ggcgcgaaaa gcgcaccttc tggctcacgg
     8161 cgctgccgct ggccgtgctg acactgacga aagatatctg ctttgcctac ggcctgatcg
     8221 cggcgttttt gattggcctt gacctgctgt ttgcagcgga cgggtctgtt aaaaaagcct
     8281 tcccgaaagc cctgctcaag gcgggcgggc tggccgtcgt cgtgctggcg gcgttcctca
     8341 gctgggggcg ctacacagcg gctgtcaccc cgacggcgga caccgccgcc agcgtcggca
     8401 gcgaggggct gagctacggc gcggtcctcg tgggcggcgt caagcaactg ctgggcatcg
     8461 gccgcaccga gaagtttgcc cagatcatgg ccgccatggg cagcgcgttc ttcacccggc
     8521 gcatctgcct tttgggcggc ggcgccattg ccgtggcggc tatcacgatg gtggccgcag
     8581 ccgcctggct ggccgccgag aaaggccccg cccgccgccg cgtgcttgcg gcgcacctgg
     8641 ggtttgcgtt ctgctttgcg gcgctgtatc tgttccactt gattctctac aactacaact
     8701 tttccgatat tgagggcctg gcgctgaagg actacgaccg ctacctggcc ccttactacc
     8761 aggcgtggat gctggcgatg ctctgcctgt tggcccgcag cgcaaaggag cagctgggcc
     8821 agctggcgct gggcggtgcg gcggcggtca tcatggccgt gttctgctgg cgcggcatcc
     8881 ctgcggccgg gttctggacc ggggccaaca gcctgtatac cctgcgcgcc gacgtgcaaa
     8941 accgcgctga cacgatgaat actgtgctca actggcccga ccgcgtgctg gtcatcagcc
     9001 agggcgacga cgccacgcgg tggtactact accgctatga gctgaccgcc caggtcgtga
     9061 acggttttgg cggcttctat ggccgcctgg gcgagacgca ggaccgctgg gacagcgatt
     9121 ttatgaacct ggtggagagc gaaaactgga cgctgtacga ctacaaggcc gtctgcgtgc
     9181 cggacacgct ggttgcctac atggccgaga aggactgcga ttatatctta atcgaccgcg
     9241 ccgacgacta tttgcagcgg gagttcagcc ccctgtttga gggtggcctg accaacgata
     9301 tgcccgcgac gctctaccat tttgagggcg cggacgccga cgtgccgttc acgctggcgg
     9361 ctgtggcaga aagcggggtg gcgtaaatga aaaaaccgct ttttaaggca cggcagctgc
     9421 tggtgctggc ttatctgctg gcggcggtgt tctgggtggt gcggtatctc gtcggctgcg
     9481 gcgtgatgct gaactacaaa atgcagggca aaatgcccca gacccatgtg gacgccgctg
     9541 accttgtgac cgagagcttc gcgccctact cctccaacga gtggtggacg ccgccggatg
     9601 atgacccggc gtggtatctt tccaccgaca gtgacccgca catttactgg caggggcagg
     9661 gatacatcga gacagtggtg ctggacgcag cgcaccgact gcccccgggc ggcgtggcgc
     9721 tgtattacct gaaacccggc cagactgact acaccgaggc gcagaaagtc tacgccaccg
     9781 tgaccgcccc cggcgtgtat acgtttgatc tgggcggcag gtgggtaacg gggctgcgca
     9841 tcgacccgga cagtgtgggt ggcgtaccga cgctgtttac cggcattgaa ctgaaccctg
     9901 cccgggcgtg gtacacccgg tttatcccca ctgccggctg gtggctggtg ctgacgtttg
     9961 ggccatgtat cgccgccgct ttcgtttccc tttttataag gaaaaaataa agcacaccgt
    10021 agggccgggt atgcccggcc ctcagccaaa aggcaaaatc gcttttgggg gaaagctgcg
    10081 ggccgcacat gtgcggcccc tgcatccctg cgcacttgta ggggggcggc gtcctcgacg
    10141 cccctaccat catcccatat gaaggagtct gtcctatgga actgctctgc gtactggctg
    10201 ccgtggcggc gctgtttggc ggctgcacgg tacttacgct gaaatgccgc gtcccggcgt
    10261 cggtggcacc gctgacggca ctgtccctca tcgtggcggt gctgacgctg gccgccatgg
    10321 cgggggtgct ctaccccatc acctgggcgg tgtatgcgct ctgcctggcg ggcggcgtgt
    10381 gggtgctggc cacccgcaaa aaccacgctg gagcggcgca aaagctgttc accccgggca
    10441 gtgtgctgtt ctggggcatg gcgctggcgt tcaccgtcta cttttttgtg cgccagccga
    10501 tggccaccga ttttgacgag ctgagccttt gggccaccgc tgtcaaaatc acgaaggtca
    10561 acaacgacct gtacgccacg gcagaattgg gcaccccctg ggccgcgacc cagaatcccg
    10621 gcctgccgct gctggcgtat ttcttccagt ttttgggcga ttatgcagat tggaaaattt
    10681 acgtctgtta tgacattttg tatttttctg tatttgccgc cgttgtcggg gctatcccgc
    10741 gcagcaaatg gcgtgtggcc gtgccgatgg ccgccgcgct gtggtgcgtg ccgttcttct
    10801 tcacgaacta caaccacact atctacctga ccaccaccta catgaccagc tacggcgatg
    10861 tacccgcggg ccttgtgttc ggcggggcgg tggccgcatg gctggcgctg cgccaagaaa
    10921 acgcccccaa gtgggccgtg ctgccgattc tggcgctgtc tgccaacatc aaggccaata
    10981 cgtttgtatt ggcgctagtg gcagcggggc tggtggctgt ggacgagtgg ctgtttgccg
    11041 acgacggcga tttcaaggcc ggtctgctcc cccgcaccgg gttcagcctg gcctgttttg
    11101 ccgcaccgat ggcaatttac tatctgtgga atgtgcgcta tgtgggctgg ctggtcagcc
    11161 gcagcgccag tgattccggt gtcggcgaga cgagcgcccc gctgtccgcc gtcgtggtca
    11221 acggcatcaa gatcctgctg gggcagtccg tcgaaggctt ttacgccgag cgcgaggccc
    11281 agttccgcac tgcgatggcc gacatgggcc accagttctg gaccagtgac ggcaagctga
    11341 gcatgatcgg gcagggccgc aatgtggtcg cgctgattgc catcgtgttt gttgtagcga
    11401 ttctggcggc agcatcgcgg cggctcaagg cgcgtatcgc cgtcatcggc gtgctgtccg
    11461 gcgtctgctt tttgggctac aatctcatgc tggcactgag ttacggcttc atcttcaagg
    11521 atttccaggc cgagcagctg accgactaca accgctacat ttacagctat tacatcggct
    11581 ggttcatcct tgcgctggcc tgcctgtcgg tggcgctgct gccgcagatc accgtcgagg
    11641 gcggggccga tggcccgaca gcggtttttg ctgcgacccg ccgccagccg cgccttgtgt
    11701 ttgagctgtt tgtgctggcg ctggcctgcg ggatgctgtt ccgccagagc cagctgattt
    11761 tgccgcagct gagtgtgctg ggctttgcga acagcgagtt caccgaccgc cgcgccgagc
    11821 gtgccgaggc cgagctggtg tgcagctatc ttgcccccga ggatcgcgtc ttttatgtcg
    11881 ggcagggcga caacggcgag ggctggttca gcgccgtgtt tgacttctac ccgattttgg
    11941 tggactattc cggcaccgtg accacggacc ccgacaccgg cgaaaccaag atgatcggcg
    12001 gcggcggcga gctaggcctg cccgagctgc agcccgccga gggtgtgaaa aacacctact
    12061 accacgcctt tacagccgag gagctggata gcatcgtgcg cggcaacggc tgcacggtgc
    12121 tgtatatcca gacgctggat gatatttttg tgcaaagcta cgccgacctg ttcaccgaca
    12181 agctggcggc ggcccagagc ggcgagacgc tgctctaccg cgtgaccgat gcgggctttg
    12241 ccccgatgca gatggaggtg agcgccccgt gaaagcgatg aaaaactggt ttgcgcggcg
    12301 cggggcactg cccatcaccg cgtacctgct ggcgctggcc gcctggctgg tgctgggcgc
    12361 ggctcacttt ggcagcgatg cgctggcgaa ggcccagggc cgcctggccg aggaaacgat
    12421 ggccgtgacc gactggcagc tggtggggct gacccaaaat gaggacggca cgctgaccac
    12481 cgtggacggc gacccgcaga tgattttgga aaatgtcgat ggccgcgtgg tgcgcaccat
    12541 cagctacacc gcgcaatttg acggcgaagc gcgggaaatg tgcctgtact acaccacaaa
    12601 agtcggcgaa gattactctg ccgaccgccg ggtgtttccc cagagcgtgg gcgagggacg
    12661 gtacatctac acgctgcccc gcaccagcct ggccgcgctg cggctggacc cctgcagccc
    12721 cgaggaaaac aagaccgtga cgctggcggt gcgtgatatc accttaaacg ccgccgacac
    12781 gctgcccgcc gtgtggcagt attttgtgcc gacatggtac caggcgtttt gtctggtgct
    12841 ctacccggcc ttggcggccg ccgccgtgag catgattttt gcggtactca aaaaagaaaa
    12901 ataattacgg aatgagcaga tgtttcactt tgggtggggc atctgctttt ttggtggggt
    12961 agagaatgca ggggccgggt atgcccggcc cctacaatct ttttggcaaa gcctttcatt
    13021 ttgcaccgtc tttttcgacg attttgcacg aatctgttgc ttttggcatt cgtgttatat
    13081 tataatagtt aacgtatggg agaatgcgct ttttcgggcg ttttctccgg tcttatgtat
    13141 aggtaaaggg accatgagcg aaaaagaaaa gatcctgata gcggacgatt ccgcaatgaa
    13201 ccgcgctatc ctgacggaga tgctgggcga cggttacgaa atccttgaag ccgagaacgg
    13261 ccgccaggcg gtgtccatca tgcagaccaa cgtcgacatt gacctgctgc tactggatat
    13321 catgatgccc gaaatggacg gctttgatgt gttggccatg atgaataaat atcactggat
    13381 cgacgatatc ccggtcatta tgatctcggc cgagaacgca tccagctatg tggagcgtgc
    13441 ctacgatctg ggcgcgactg actacatcag ccgcccgttt gatatggcca tcgtgcgccg
    13501 ccgcgtcatc aacaccctga tgctctacgc caagcagaag cgcctggtgc ggctggtggc
    13561 cgagcaggtc tacgaaaaag aaaagagcag ctcgacgatg atcaacatcc tgagccatat
    13621 cgtcgagttc cgcaacggtg agagcggcct gcacgtgctg cacatccaga cggcgaccga
    13681 catcctgctg cgcacgctgg tccggaaaaa cgacaaatac aatctgaacg ccgccgatat
    13741 ctcgctgatc agcacagcgt cggcgctgca cgacatcggc aagatcaaca tccctgcgtc
    13801 gattctgaac aagcccggca aactgacgaa ggaagagttt gacacgatga aggcccacac
    13861 caccaccggt gcggaaattc tcgacaaact ccccttccag caggagtcgc cgctggtcaa
    13921 gacagcctac gccatctgcc gctggcatca cgagcgctgg gacggccgcg gctaccctga
    13981 cggcctgaag ggtgaggata ttcccattgc cgcgcaggtc gtggcaatgg ccgacgtcta
    14041 cgacgccctg acgagcgagc gctgctacaa aaaagccttt ggccatgaca aggccatgga
    14101 gatgattttg aacggcgagt gcgggcagtt caacccgctg ctgctggaat gcctgacgga
    14161 tgcgggtgcg cggctgcacg ccgagctgag cctggcatcc tccggcggaa accgccgcaa
    14221 ctacctggcc gaggcacagc atatctcgga ggtgctgctg cacgagaaat cgctgcccgg
    14281 gcaggatcac atcctgcgca cgctgtccgt gctgcgcacc aaggcgaatt tctttgccga
    14341 aaacgcccgc tgtgtgcagt ttgattacag cgaggccacc ggcctggtgc ggttctcacc
    14401 ctgggcggtg gagcatatgc aggtgccgga agagctgcat ctgccggacg aagccgatcg
    14461 atccggcttt gccatcgcgg atattgatcg tatccgtacc gcgctgcggg ccacgacagt
    14521 gaaccgcccc cacgctgagc tttcgatgct gctgcccatt gacggcgagc tgcgctggca
    14581 tcatgtgtcg ctctgcgccc tgtggagcga tgagataccg cccctttaca tcggcgcggt
    14641 gggccaggcc aaccccgtgt aacaaggggc agcgcaacgc ctgtgtcact gcggaattta
    14701 ccgctgaaga ataaaagtga aaatgagaaa ggcagaaatg atatgacgtt acaggaattt
    14761 tatgcccgcg tcggcggcga ttacaatgcc accctgagcc gtctgcccag cgaggccctt
    14821 gtgaagaagt ttatcatgaa ataccccggc gaccccagct ttgcccagct gaaggacgcg
    14881 ctggccgccc aggattggga gctggctttc cgcgccagcc acacactgaa gggcgtggcc
    14941 cagaacttag ggatggaccg cctgtacaag gctgccgcgg ccctgtgcga cgccgtgcgc
    15001 ggccccaagc cgctggaaga tgagagcctt tggccgcccg tccttgctgc ccacgaggaa
    15061 gtgctggcgg caatccgcga actgtaaagc cccccttgca ggtcggccct gcagaatact
    15121 atgtgcggcg tctctgccgc tgcacaggaa accggagtgc ccctgccatg tataataaaa
    15181 aggagaacct ccccactctg cggcgcacgg tctgctgcct gctgcttacc agtatgctgt
    15241 tggcagcgat gacacgcccc ggcgcaccct gcgcgtaggc ttttacacct acacgggcta
    15301 ccatgtcatg gacgaggacg gccaccgcag cggctattcc tacgaggtac tgcagcgcat
    15361 tgcccagcat gagaatgtac agtttgagta tctggcttac gactgtgatg ccaacgaggc
    15421 cgccgatatg ctggagcgcg gcgagattga cctgctgccc acgctgcgca agaccgacga
    15481 gcgcgccgag cgcttcgact tctccaacga gccgatctcc aatgtagcca caatgctgac
    15541 cgtcaaggct ggcaaccgca gcatcgtggc gggcaactat gatacgtttg acggcatggt
    15601 cgtcggtatg agccgcagcg gcaacggccg caacgagagt ttcaaggcct acgctgagaa
    15661 aaacggcttt acctacacgc cggtctattt tgacaccgat gaggaacttt ccgccgcact
    15721 gcgcagcggc accattacgg cggcagtctc caaccggatg cgccagacct ccaacgaatg
    15781 ggtcatcgac acctttgaca atgaggacgt ctacattgcc gtgcgcaaag gcgatgcttc
    15841 gaccctgcgg cttgtcaacg acgcgttgat ccgtatggac cgtgacgacc ccacctggcg
    15901 cacgaccctg tttgacaagt acaacaagaa catccacacc agcaacaagc tctacctgac
    15961 cgctgctgag gaaaactaca tcacggccca caatgcagcg gacaaggagt tcaccgtgct
    16021 ggccaacccg gaccgctacc cctactccta tctggacaag gacggcaagc tgaccggcat
    16081 catggtagac ctctttaagc tcgtggcggg ccgcgcccgg ctgcactaca agttcttgac
    16141 accggctgac cgcaccgagt acaagcagat tctcaatgac cgcgcggcgg attttgtcat
    16201 tgatttggcc agcgattttt ccaccgccga ggattgcggc tacaaactga cggacagcta
    16261 cctctcggcg gagttttcct gggtgttgct gcgcagccac gacggcgacc tgcgcagggt
    16321 ggccgtggcc tacaacttca acaccgacgt gctggagctg cccggcctgg acgactgcgc
    16381 cactgtcgag tacatggact ccttcgatg
//
LOCUS       DBMDSQ010000014        16335 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_1132_length_16335_cov_2.135456, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000014 DBMDSQ010000000
VERSION     DBMDSQ010000014.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 16335)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 16335)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16335
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1132_length_16335_cov_2.135456"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            101..943
                     /locus_tag="ACZPAX_01120"
     CDS             101..943
                     /locus_tag="ACZPAX_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002609003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HIR1112529.1"
                     /translation="MFTIIKRRELNPTVTELWIHAPLIAKKAKAGQFIIVRAKEDSER
                     IPLTIAGFDREAGTVSIIFQVVGAGTMQLNSLKEGEAVHDFVGPLGKATEIEGLKNVC
                     VVGGGVGCAIALPIAQALHEQGTKVTGIVGFRNKDLVILEDEFRACCDEFIIMTDDGS
                     YGEKGVVTAPLEQRIVDGANFDEVITIGPLIMMKFVVKTTKPHGVKTVVSMNPIMVDG
                     TGMCGGCRLTVGGQTKFACVDGPDFDGFEVDFDEAMHRGTMYKPFEAHAREAECNLLK
                     QEVQ"
     gene            946..2343
                     /gene="gltA"
                     /locus_tag="ACZPAX_01125"
     CDS             946..2343
                     /gene="gltA"
                     /locus_tag="ACZPAX_01125"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HIR1112530.1"
                     /translation="MPNMDPKKCPMPSQEPNVRNKNFKEVALGYTEEMAVNEAKRCLQ
                     CKNHPCRSGCPVEIDIPGFIKHVAEGDFEAAYNVIAQSSALPAVCGRVCPQEHQCEGK
                     CVRGIKGEAVGIGRLERFVADWYRNNVHTKPTAPASNGHKVAVIGAGPSGLTVAGDLA
                     KLGYKVTVYEALHVAGGVLMYGIPEFRLPKDIVQHEVEGLKELGVDIETNMVIGKVLT
                     IDELMNDYSFEAVYVASGAGLPRFMGIPGESLNGVYSANEYLTRVNLMKAYKEDSRTP
                     IMKSKSVAVVGGGNVAMDAARCAKRLGAENVYIVYRRGMAELPARKEEVEHAEEEGIV
                     FKTLTNPTEVLGDENGWVKGMTCVEMELGEPDASGRRRPIVKEGSEFVLDVDTMIMAL
                     GTSPNPLIRSTTPGLDADKHGCLITNGPDGLTSRPMVYAGGDAVTGAATVILAMGAGK
                     EAARAIDAAIKAKEQ"
     gene            2629..3768
                     /locus_tag="ACZPAX_01130"
     CDS             2629..3768
                     /locus_tag="ACZPAX_01130"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HIR1112531.1"
                     /translation="MYHPFFQFVLPRLGQAALDILLYLNFLQLPPLQLAAQWPVLYYG
                     LPLVLMALLAYISRDPLGLGIVFVGHLVTFYCIGTGIGLVLRRVGGAPLTVWHIFWLG
                     GITPWLLTGFIMWWGRRRALRLCTTKYNLTTQKALPNGQLRIVQISDVHPRACAAMDR
                     TRIPELREKVAACQPDLLVLTGDIFDEYTEKEDFDAFCQFFGEIDAPLGKYYVLGNHD
                     LFHHWREPSFGRADLECAFAAAGVRILEDTAITLSCGVRIVGRKDYLYTNGSRFTAAQ
                     LMPTGPDDRYTVWLDHEPRDFKNAAAAGADLILSGHTHGGQVWPAGAVGMAAKNERNY
                     GKKHITDRCDAIVSGGTGTWGYKFRTQGRTEIVCVTITTEKETSQ"
     gene            3765..5102
                     /locus_tag="ACZPAX_01135"
     CDS             3765..5102
                     /locus_tag="ACZPAX_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019162555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HIR1112532.1"
                     /translation="MNKRERLGSRLGFIMLSAGCAIGCGNVWKFPWMCGQNGGGSFML
                     LYILCLLVLGLPAMVMELAVGRAAQASPIYMYQRLEKPGHKWGVFGVFSLIGNIAIMA
                     FYTVVTGWILYYFVKFLTGDNADFGFAQMLADPKVNVTYLFAVVVAAFVLLSFNLQGG
                     LERVTKYMMTALLVLMLVLAIHSLTFAGAAEGLRFYLVPDFSKINGSVIVAAMNQAFF
                     SLSVGMGGMAIFGSYIGKERSLMGESLHIIALDTFVAVLAGIIIFPACFTYGLEVTAG
                     PSLLFDTMAGVFNNMAGGRWWGALFFLFMVFAALSTVLGVCENILAMVRELTGWTRPK
                     GCVVCGAGIFLLALTTALGYSVLHFQPFAPGSAWLDFWDFIVSNNVLPLGTLVLALFC
                     CNRLGWGWENFVNEANTGKGLKVQPWMKPVFRYFVPCAIAFIYVYGMVTFHWR"
     gene            complement(5172..8204)
                     /locus_tag="ACZPAX_01140"
     CDS             complement(5172..8204)
                     /locus_tag="ACZPAX_01140"
                     /inference="COORDINATES: protein motif:HMM:NF024486.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HIR1112533.1"
                     /translation="MNNVTRSIFRAIHEGKWLSIEYKNQQTQQTKYWVAVKGLNPRTR
                     TLTVDGLHLKLLTVQDLTIHIDRIQAAEVVDGSWCPVNETLVADIRDNPGKYTALFAN
                     SANLRVLDYLAACNRLDATPYQTNYSLIHAIDADRFDEGAEDTIELGDDQFRELVQRF
                     QRRVDDRRQNAARPSLPQLGLNLISINTNKGLYVLAYRPLRLDVRRRTLRADADPVIC
                     REFTINGAKLSIHQFLDADDHALLDDFYKNQETIKDRITRGNPQVRGVDDMPYLIAIG
                     RDCPVDLEHEYRGILNMFEDPSGETVTAPLRAFFGEMTARPRRRKSYPLALLNNKVNL
                     DQLLAINNAMRYPLAYVQGPPGTGKTNTIVNTLTTAFFNERTVLFASYNNHPIDGVVE
                     KLQQIDYHGHTVPFPILRLGNAEKTAEALRTIAKLYDQCIQLPVPEKLLDKNHADRTA
                     RAKQLTELLERYERVLDLRERKETIERLLEARSQMNFRFELEAGQLPQVNRELAAYGD
                     IDTADAMALLDRNEGELLRYLYYTSVQYIRRLAEPKYEDLMEIVRSADNDKEKAAAFN
                     KYISEPENLKKLLRVFPIVATTCISAHRLGDPEPSFDMVIMDEASQCNTAMSLVPILR
                     GRSLMLVGDPQQLSPVILLDPADNKALRRKYSVTEEYDYIENSIYKCFLACDAVSDET
                     LLSYHYRCSPRIIEFNNRKYYNHKLHIASQEHNPEPLVYVDVPDNTTDERNTAPQEVR
                     RIDAYLSRHPDKQVGIITPFARQRAAIEAMLRANHYDNASCGTVHAFQGDEKDVVIFS
                     LALTDQTREKTYAWLKTNKELINVATSRARDQLVILSSQKELDRLHAGAVDDDIYELV
                     QYVRTNGVSDVTEKPAASRALGIKPYSTRTEDAFLENLNHALDNAFLDGSRCTVRKEV
                     PIAQVFENNPSYTDLFYTGRFDFVVYQREGRRLMPILAIELDGREHRDDPAVQRRDRK
                     KEQICREHGFELIRVENSYARRYNYIKSILINYFRKVS"
     gene            8519..9340
                     /locus_tag="ACZPAX_01145"
     CDS             8519..9340
                     /locus_tag="ACZPAX_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015564709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding periplasmic protein"
                     /protein_id="HIR1112534.1"
                     /translation="MKHHRPRLLFLLAVLCLAVCLLAGCAARAATADPDADLPTIVIG
                     SDSYPPFNYLSADGQPTGIDVELATEAFRRMGYKAQFVTIDWVDKKELVENGTVDCLW
                     GSFSIDGREDEYRWAGPYMVSRQVVAVMPGSDIETLSDLAGKTVAVQATTKPEALFLD
                     GGDPRIPEIRALLSMQKRELIYAYLSKGYADAVAAHETSILQFMSDFGIEYRILDEPL
                     QVVGLGVAFALNDDRGLDTALNAALADMRTDGTTRAILAKYLPDPDKYLEVDYGR"
     gene            9330..11504
                     /locus_tag="ACZPAX_01150"
     CDS             9330..11504
                     /locus_tag="ACZPAX_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005922102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR1112535.1"
                     /translation="MAAKRLPRLNGFWLLSVLIGVLLLVSISFYSANTDIATAEDALS
                     TRVDYIKEQCADYSSLNLASEAKSLLRVTESAQQCARDIWYLTEKPDDLYPAAEDVLP
                     HFVENHYLTGLLLLSRDGQITKSYCKEEALLPLLNKVLTTDSLLDVADHPKKTYAVRL
                     ACGDDSFLDIAASAREGADCIVVAFYHTPAEYVHTYKLSYQHLVSGFHIATDGTLAVA
                     SGNKIIAANDESLLGLSTDEVAPLQLIKQRGVYDRMVYVRDTGMGTSGYFGYTEKGRD
                     CYVYAFLPEAAVFTTTPKNIIFVVISYTLIILLIELVRRRTMHSYQEEKLLQEKAYQK
                     SLEKAARKAEAANVAKTEFLQRMSHDIRTPINGIRGMVEIGDHYADDMTKQAECRRKI
                     WDASGFLLELVNEVLDMGKLESGEVVLEHRPFNMVKLLTGLQDVLEKQAAGRGIRILC
                     DDIDLPHPDLIGSPIHVKRLMMNIMSNAVKYNKDNGTITMTLREERCDGKTAWVKFTC
                     ADTGIGMSEEFQKHIFEPFTQEVLDARSTYGGTGLGMPIAKSLVDKMGGTITFESRQG
                     VGTTYYITLPLEIDPNAAPQPETEKPADDATLQGVNILLAEDNDLNLEIAQFLLENAG
                     ATVTAARNGQEAVDIFAASRPGEFDAILMDVMMPVMDGYTATRTIRRLDRPDAGRIPI
                     LATTANAFAEDRRRACEAGMNEHLTKPLETELVIKTLAKYRK"
     gene            complement(11591..12178)
                     /locus_tag="ACZPAX_01155"
     CDS             complement(11591..12178)
                     /locus_tag="ACZPAX_01155"
                     /inference="COORDINATES: protein motif:HMM:NF012655.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR1112536.1"
                     /translation="MQNDQQAARKAAVYEAALQLIGQGVSPAALKIQQLADAAGIGKG
                     TVYEYFSSKEEILQGLAEYCFARENERVRAMFADCTTLAGLEERITGYLQDIAASRMG
                     SYKMIAETLGMPRRTGAMPACMAELKEIVSELLARLQAAGEIAPELGMEYCYMSLLSA
                     VVGGLMGLCYAADHDRTVEDLMDNLRLMIRRTLGK"
     gene            complement(12168..>16335)
                     /locus_tag="ACZPAX_01160"
     CDS             complement(12168..>16335)
                     /locus_tag="ACZPAX_01160"
                     /inference="COORDINATES: protein motif:HMM:NF013069.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR1112537.1"
                     /translation="DSAMVKASTAVNQLGDALPDMASTPTLMEISPDMMATQYVAVDY
                     DGMDIYELSDYVSENVLPQLERVDGVASVSTTGLVEKSVQITLDQDKIDAVNDKLLVK
                     VSDRLADAKKELDDNKKKINDGLKELDDAQAQLDAGKSELNTQKADVTTQLRDAITEL
                     DKQIPELEKKIADLKTDIDDANRKLEDAKNADTTLPEVTLPIDDALFASCKQILAAWD
                     SQYNANELPANLAEAKGSTAKMGAMVNAIERATQNINSTAAAENLLESGMDVAQSAAY
                     LDAQYTALTGRIQALDAEIAGLEDDKAAAEGDEAQQQAIQAQIDAKRAEQAGYQKILD
                     DNAPRRARLTELNGCLTTLSTTSTALNNATVLLQARQQAEAGLNEGMKPLQQTLNETV
                     QALNDQLTKAQEILTQLNDQRAKMESALASLGDPAVDPAMADAAVQLLLSGSEAQLQL
                     GEFQISSGRTQLEAGKTQLEAAQEEYDSAREEALKNANLDQLLNMATLTQLIAAQNFS
                     MPAGYIDGGAGDDNRYIIKVGDAFADTDELKNMVLCNIDGIGDVRLADVAEVEMTDNA
                     DDAYAKVDKNRAVLLSIYKSSTASTSTVSNAANAAMEEMMAETEGLHLTPIMDQGDYI
                     KLIVNSVLSNLIEGAILAIIVLALFLKDLRPTIVVAISMPLSVLFAIVWMYFTGITFN
                     ILSLSGLALGVGMLVDNSVVVIENIYRLRGRGVPAPRASVQGARQVAGSIVSSTLTTI
                     CVFLPLVFTTGMVRELLSDMAWTITFSLLASLIVALTVVPCAGSTVLKKQKDIKHPWF
                     DKALNGYEKLLRFCLRRKAVPLTLAIVLLGVSIWRVATMGVVMIPDMSSNQLTVTVNV
                     PDDTAKEDAFTTADSVMDAIMTVDGVDTVGAMSGSPNSMVMMAGSSAATDNTAFTYYV
                     LLGDKAPKAKVVEQEIYDKTADLNCEIDVNSNGMMDMSALSGSGIEVDLYGNDLDDLS
                     AAGKQVIEAMNGIDGIVNPTDGQDAGDAEVNITIDKDKAMRMGLTVAQVYQQIAAKLT
                     TDTTATTLKAGGTSYTVTIVDKTDTPTLDDIFNMEFETTTTDEDGNTVKETHTLGEFA
                     TRTTTAGYTEISRENGSRQLSITSETAEGYNTTLISRELEPKLAELQLPDGVTAELAG
                     ETTQVTEMLGQMAKMLALALLFVYFVMVAQFQSLLSPFIVLFTIPLAFTGGMLGLMAT
                     GEQLSLISLMGFLVLMGVVVNNGIVFVDYANQLRIGGLERSDALVATGRTRMRPILMT
                     TLTTVLAMVTMLFGTDMASEMSTGMAIVIIGGLSYATLMTLFIVPVLYDLFYRKPPVN
                     IDVGDDGMDDLPDDAAEFAAEFAARRAAENAAKAPAEEKRRPDLSRAAHAPAENETPN
                     EEKEADHDAE"
BASE COUNT         3001 a         4936 c         4973 g         3425 t
ORIGIN      
        1 tctcttctca ttctaacaga tatttcatct ttacattttc gcggaaatac gctacaatat
       61 aaatgtataa atataaaaac aaagagaagg agagatcgct ttgtttacta tcatcaaacg
      121 gcgggagctg aaccccaccg tgaccgaact ctggatccat gcgccgctca tcgccaaaaa
      181 ggcgaaggcc ggccagttta tcattgtccg cgcaaaggag gactccgagc gtatccccct
      241 gaccatcgca ggctttgacc gcgaagctgg caccgtttcc atcattttcc aggtcgtcgg
      301 cgcaggtacc atgcagctca actccctcaa agagggcgaa gccgtgcatg actttgtggg
      361 acccttgggc aaggccaccg agatcgaggg tctgaaaaac gtctgcgttg tcggcggcgg
      421 cgtgggctgc gccatcgcgc tgcccatcgc ccaggcactg cacgagcagg gcaccaaggt
      481 caccggcatt gtcggcttcc gcaacaagga tctggtcatt ctcgaggatg agttccgcgc
      541 ctgctgcgat gagtttatca tcatgacaga cgacggctcc tacggcgaaa agggtgttgt
      601 cactgcgccg ctggagcagc gcattgtgga cggcgcaaac tttgacgagg tcattaccat
      661 cggacccctg attatgatga aattcgtcgt caagacgacg aagccccatg gcgtcaagac
      721 tgtcgtctcg atgaacccca tcatggtcga tggcaccggt atgtgcggcg gctgccgcct
      781 gaccgtgggc ggccagacca agtttgcctg cgtggacggt cccgactttg acggctttga
      841 ggtcgatttt gacgaagcca tgcaccgcgg cacgatgtac aagccctttg aggcacacgc
      901 ccgcgaggcc gagtgcaatt tgctgaagca ggaggtgcag tgattatgcc gaacatggat
      961 ccgaaaaagt gccccatgcc cagccaggag ccgaacgtcc gcaacaaaaa ctttaaagag
     1021 gtcgcccttg gctacaccga ggagatggcc gtgaacgagg ccaagcgctg cctgcagtgc
     1081 aagaaccacc cctgccgcag cggctgcccc gttgaaattg atattcccgg cttcatcaaa
     1141 cacgttgccg agggcgactt tgaggccgcc tacaacgtca ttgcgcagtc cagcgcgctg
     1201 cccgccgtct gcggacgtgt ctgcccgcag gagcaccagt gcgaaggcaa gtgcgtgcgc
     1261 ggcatcaagg gcgaggccgt cggcatcggt cgtctcgagc gctttgtcgc cgactggtac
     1321 cgcaacaacg tacataccaa gcccaccgcc cccgcctcca acggccacaa ggtcgctgtc
     1381 atcggcgctg gcccgtccgg cctgaccgtc gcgggcgacc tggccaagct cggctacaag
     1441 gtcaccgtct acgaggccct gcacgttgcg ggcggcgtgt tgatgtacgg catccccgag
     1501 ttccgtctgc ccaaggacat cgtccagcac gaggtcgagg gcctgaagga gctgggcgtt
     1561 gacattgaaa ccaatatggt catcggcaag gtgctgacca ttgacgagct gatgaatgac
     1621 tacagctttg aggctgtcta tgtcgcgtcc ggtgccggtc tgccccgctt tatgggcatc
     1681 cctggcgaga gcctgaacgg cgtttactcc gccaacgagt acctgacccg cgtgaacctg
     1741 atgaaggcgt acaaggagga ttcccgcacg cccatcatga agagcaagtc tgtcgccgtt
     1801 gtcggcggcg gcaatgtcgc catggacgcc gcccgctgcg ccaagcgctt gggtgccgag
     1861 aatgtgtaca tcgtctaccg ccgcggcatg gccgagctgc ctgcccgtaa agaagaggtc
     1921 gagcacgccg aggaggaggg catcgtcttt aagacgctga cgaaccccac cgaggtgctg
     1981 ggcgacgaga acggctgggt caagggtatg acctgcgttg agatggaact gggtgagccg
     2041 gacgcctccg gccgccgccg ccccatcgtc aaggaaggca gcgagttcgt tctggacgtt
     2101 gatacaatga tcatggcgct gggcaccagc cccaacccgc tgatccgctc caccacgccg
     2161 ggtctggacg ccgacaagca cggctgcctg atcaccaacg gtcccgacgg cctgacgagc
     2221 cgcccgatgg tctacgccgg cggcgacgcc gttaccggtg ccgccacggt cattctggcc
     2281 atgggtgccg gtaaagaggc tgcaagagcg attgacgcgg cgattaaggc gaaggaacag
     2341 taaaaaataa aattcagccc ccgtgccgcg aggtgcgggg aaatttttgt taggaaaggc
     2401 ttccccgtgg ggctgcgccc gcaggcgcgt gtcggagtgc aaccgccgta gacggcactt
     2461 ggcacggaga ctgaagctgg ccgcgaagcg gactgatggg gggcgaactt tgccatacta
     2521 tcccctgtat gggtaatcac ggtatctccg cccctcatcc gtctgcgggc gggaccgcag
     2581 acaccttccc ccaaggggga aggcattctc acaacaaagg ctgacaccat gtatcatcca
     2641 ttcttccaat ttgttcttcc ccggctgggg caggcggcgc tggatatttt gctgtatctc
     2701 aactttttac agctgccgcc gctgcagctt gcggcccagt ggccggtgct gtactacggc
     2761 ctgccgctgg tgctgatggc gctgctggct tatatcagcc gggacccgct gggccttggc
     2821 atcgtctttg tcgggcatct cgtcaccttc tactgcatcg gcaccggcat cgggctggtg
     2881 ctgcggcgtg tcggcggtgc gccgctgacc gtttggcata ttttttggct gggcggcata
     2941 acgccgtggc tgctgaccgg gtttatcatg tggtggggcc gccgccgggc gctgcggctg
     3001 tgtaccacaa aatataacct gacaacgcag aaagccttgc ccaatggtca gctgcgcatt
     3061 gtacagatca gcgacgtaca cccccgggcc tgcgcagcca tggaccgcac ccgcatcccg
     3121 gaactgcggg agaaagtcgc ggcctgccag ccggatctgc tggtgctgac cggcgacatc
     3181 ttcgacgagt acaccgagaa agaggatttc gacgccttct gccagttctt cggcgagatc
     3241 gatgccccct tgggcaaata ctatgtgctg ggcaaccacg acctgtttca ccactggcgg
     3301 gagccgagtt ttggccgcgc cgacctggag tgcgcctttg ccgcagcggg tgtccgcatt
     3361 ctggaggaca ccgccatcac gctgtcctgc ggcgtgcgca tcgtgggccg caaggactac
     3421 ctgtacacca acggcagccg ctttacggct gcacagctga tgcccaccgg cccggacgac
     3481 cgctacacgg tctggcttga ccacgagccg cgggacttta agaacgccgc cgcagcgggg
     3541 gccgatctga tcctgagcgg ccacacccac ggcgggcagg tctggcctgc gggcgcggtg
     3601 ggcatggctg ccaaaaacga gcgcaactac ggcaaaaaac atatcacaga ccgctgcgat
     3661 gccatcgtca gcggcggcac cggcacctgg ggctacaagt tccgcaccca gggccgcacc
     3721 gagatcgtct gcgtaaccat cacgactgaa aaggaaacga gccaatgaac aaacgcgagc
     3781 gcctgggcag ccgcctgggc tttatcatgc tgtccgccgg gtgcgccatc ggctgcggca
     3841 atgtctggaa attcccctgg atgtgcggcc agaacggcgg cggcagcttt atgctgctgt
     3901 acatcctctg ccttttggtg ctgggcctgc ccgccatggt catggagctg gccgtgggcc
     3961 gcgcggcgca ggcaagcccc atttatatgt accagcgtct ggaaaagccc ggccacaaat
     4021 ggggcgtgtt cggcgtgttc agcctgatcg gcaacattgc catcatggcg ttttacacgg
     4081 tcgtcaccgg ctggattttg tactattttg tcaaattcct gactggggac aacgccgatt
     4141 tcggctttgc gcagatgctc gccgacccga aagtcaacgt gacctatctg tttgcggtgg
     4201 tcgtggcggc gttcgtactg ctcagcttca acttgcaggg tgggttggaa cgcgtgacca
     4261 agtacatgat gacagcgctg ctggtgctga tgctggtgct ggccatccac agcctgacct
     4321 ttgccggagc cgccgagggg ctgcggttct accttgtgcc ggatttttcc aaaatcaacg
     4381 gcagcgtcat cgtggcggcc atgaaccagg cgtttttttc gctgtccgtc ggcatgggcg
     4441 gcatggcaat cttcggcagc tacatcggca aggagcgctc cctcatgggc gagtcgctgc
     4501 acatcatcgc gctggacacc tttgtggcgg tgctggcggg catcatcatc ttcccggcct
     4561 gcttcaccta cgggctggaa gtcactgccg ggccaagcct gctgtttgac acaatggccg
     4621 gtgttttcaa caacatggcc gggggccgct ggtggggcgc actgttcttt ctgttcatgg
     4681 tgtttgcggc gctgtctacc gtgctgggcg tctgcgagaa catcctggcc atggtgcgcg
     4741 agctgaccgg ctggacacgc ccgaaaggct gtgtggtttg cggcgcgggt atctttctgt
     4801 tggcgctgac gacagcgctg gggtacagcg tactgcactt ccagcccttt gcgccgggca
     4861 gcgcatggct ggacttctgg gatttcatcg tcagcaacaa cgttctgccg ctgggaacgc
     4921 tggttttagc tctcttctgc tgcaaccgcc tcggctgggg ctgggagaat tttgtaaacg
     4981 aggccaacac cggcaaaggg ctgaaagtgc agccctggat gaaacctgtg ttccggtatt
     5041 ttgtgccctg tgccattgcg tttatttatg tgtacggcat ggtcacgttt cattggcggt
     5101 aagccgcagc aaaaaaagac ctgcgtcctg atgggatgca ggtctttttt gtgcagggct
     5161 gggggcgggc gttacgacac tttcctgaag taatttatca aaatactttt aatataatta
     5221 taccgtctcg catagctgtt ctccacgcgg atcagctcaa acccgtgctc acggcagatc
     5281 tgctccttct tgcggtcacg gcgctggacg gcggggtcgt cgcggtgctc gcggccgtcc
     5341 agctcaatgg ccagaatcgg catcaggcgg cggccctccc gctggtagac gacaaaatca
     5401 aaccgcccgg tgtaaaacag atcggtgtag ctggggttat tctcaaacac ctgggcaatg
     5461 gggacttcct tgcgcacggt gcagcggctg ccgtccagga aagcgttgtc cagcgcgtgg
     5521 ttcaggttct ccaaaaaggc gtcctcggtc ctggtgctgt acggcttgat gcccagtgcc
     5581 cggctggcgg cgggcttctc ggtcacgtcg gacacgccgt tggtgcgcac gtactgcacc
     5641 aactcgtaga tatcatcatc caccgcgccc gcgtgcaggc ggtccagttc cttctggctg
     5701 gacaggatga caagctggtc ccgcgcgcgg gaggtcgcca cgttgataag ctccttgttg
     5761 gtttttagcc aggcgtaggt cttttcccgg gtctggtcgg tcagcgccag cgagaagatg
     5821 accacatcct tctcgtcgcc ctggaacgcg tgtaccgtgc cgcagctggc gttgtcgtag
     5881 tggttggccc gcagcatcgc ctcaatggcg gcacgctgcc gcgcaaacgg cgtgatgatg
     5941 cccacctgct tatccgggtg gcggctcaag taggcgtcga tgcggcgcac ctcctgcggg
     6001 gcagtgttgc gctcgtcggt agtgttgtcg ggcacatcca catagaccag cggctcgggg
     6061 ttgtgctcct ggctggcaat gtgcagcttg tggttgtagt atttgcggtt gttgaactca
     6121 atgatgcgcg ggctgcaccg gtagtggtag cttaacagcg tctcgtcgct gacggcatcg
     6181 caggccagga aacacttgta aatcgagttt tcgatgtagt cgtattcctc tgtcacgctg
     6241 tacttgcggc gcagggcctt gttgtcggcg gggtccagca gaataaccgg ggaaagctgc
     6301 tgcgggtcgc cgaccagcat cagcgaccgg ccgcgcagaa tcggcaccag cgacatggcc
     6361 gtgttgcact ggctggcttc gtccataatg accatatcaa agctcggctc ggggtcgccc
     6421 aggcggtggg cggagatgca ggtcgttgcc acgatgggga aaacgcgcag cagctttttc
     6481 aggttttccg gctcggagat gtacttgttg aaagccgctg ccttctcctt atcgttgtcg
     6541 gcgctgcgga caatctccat caaatcctca tatttcggtt cggccaggcg gcggatgtac
     6601 tgcaccgacg tgtagtagag gtagcgcaaa agttcgccct cgttgcggtc cagcagggcc
     6661 atggcgtcgg cggtgtcaat atcgccgtag gcggccagtt cgcggttgac ttggggcagc
     6721 tgcccggcct ccagctcaaa gcggaagttc atctggctgc gggcttccag caggcgctcg
     6781 atggtctcct tgcgctcccg caggtcaagc acccgctcat agcgttccag cagctcggtc
     6841 agctgtttgg cgcgggctgt gcggtcggcg tggtttttgt ccagcagctt ttccggcacc
     6901 gggagctgga tacactggtc atacaatttg gcgatggtcc gcagcgcttc ggcggtcttt
     6961 tcggcgttgc caagccgcaa aatcgggaag ggcaccgtat ggccgtggta gtcgatctgc
     7021 tgcaattttt ccaccacgcc gtcaatcggg tgattgttgt aactggcaaa aagcaccgtg
     7081 cgctcgttga aaaacgccgt agtcagcgtg ttgacgatgg tgttggtctt gccggtgccc
     7141 ggcggcccct gcacataggc caggggatag cgcatggcat tgttgatggc aagcagctgg
     7201 tccaggttga ccttgttgtt cagcagcgcc agcgggtagg atttgcggcg tcggggccgg
     7261 gccgtcatct cgccaaagaa agcccgcagc ggtgcggtga ccgtctcgcc ggagggatcc
     7321 tcgaacatat tgaggatgcc gcgatactcg tgctccaaat ccaccgggca gtcgcgccca
     7381 atggcgatca ggtagggcat atcgtccacg ccgcgcacct gcgggttgcc ccgggtgata
     7441 cgatccttga tggtctcctg atttttgtaa aagtcgtcca gcagcgcgtg gtcgtcggcg
     7501 tccagaaatt ggtggatgct cagctttgcg ccgttgatgg tgaactcccg gcagatgacc
     7561 ggatcggcgt cggcccgcag cgtgcggcgg cgcacatcca gccgcagcgg gcgataagcc
     7621 agcacataca gccctttgtt ggtgtttatg ctgataaggt tcagccccag ctgcggcaga
     7681 ctgggccggg cggcgttctg cctgcggtca tccacccggc gctggaaccg ctgcaccagc
     7741 tcccggaact ggtcgtcccc cagctcgatg gtgtcctcgg ccccctcgtc aaagcgatcc
     7801 gcgtcgatgg cgtggatgag gctgtaattg gtctggtagg gcgtggcatc cagccggttg
     7861 caggcggcca ggtagtccag cacccgcaga ttggccgagt ttgcgaacag cgctgtgtat
     7921 ttgccgggat tatcccggat atcggcaacg agggtctcat tcaccgggca ccaggagccg
     7981 tccacaactt cggctgcctg gatgcggtca atgtggatgg tcaggtcctg gacggtcagc
     8041 agtttcaggt gcaggccgtc caccgtcagc gtgcgggtgc gggggttcag ccccttgacg
     8101 gcgacccagt attttgtctg ctgggtctgc tggtttttgt actcgatgct gagccacttg
     8161 ccctcatgga tggcgcggaa aatgctgcgt gtgacgttgt tcatcttccg ctcctttccg
     8221 ctgtgatggc aaaattttac attgttattg ctattataac atcacacccg caccggggca
     8281 actgttttgc cgaaacaaac gcggctgatc ggcacaggcg acgcatatcc tggtatgatg
     8341 tggtttgcaa aggttgcagg gcggccagtc ccctggccgc cgcaaagttt cttctatttt
     8401 ttgtcaaatc atgcagctgc ctttcttgac tgtatcggca tccgatgcta caataaatat
     8461 tattttccca aagtgttact ctctgtaata ttccaacaaa tatagaggat atttgcgtat
     8521 gaagcaccac cgccccagac tgctgttttt gctggccgtg ctctgcctgg ccgtctgcct
     8581 gctggcgggc tgcgccgcgc gggccgccac agccgacccg gatgctgacc tgcccaccat
     8641 cgtcatcggc agtgacagct acccgccctt caactacctg agcgccgacg gccaacccac
     8701 cggcatcgat gtggagcttg ccaccgaggc attccgccgc atgggctaca aggcgcagtt
     8761 cgtcaccatc gactgggtcg acaaaaaaga gttggtagag aatggcaccg tcgactgcct
     8821 gtggggcagc ttttccatcg acgggcgcga ggatgagtac cgctgggccg ggccgtacat
     8881 ggtcagccgc caggtcgtgg ccgtcatgcc gggcagtgat attgagaccc tgtccgacct
     8941 ggcgggcaag accgtcgccg tgcaggccac caccaagccg gaagcgctgt ttctggatgg
     9001 cggcgatccc cgcatccccg agatccgcgc cctgctctcg atgcagaaac gcgagctgat
     9061 ctacgcctat ctgagcaagg gctacgccga cgctgtggcc gcccatgaga cctcgatttt
     9121 gcagttcatg tcggatttcg gcatcgagta ccgcatcctg gacgagcctt tgcaggtggt
     9181 ggggctgggc gtggcctttg ccctgaacga tgaccgcggg ctagataccg cgctgaacgc
     9241 tgccctggcc gatatgcgca ccgacggcac gacccgggcc atcctggcta agtacctgcc
     9301 cgacccggac aaatatctgg aggtggacta tggccgctaa acgtctgccc cgtctcaatg
     9361 ggttctggct gctgtccgtg ctgatcgggg tgctgctgct cgtctccatc tctttttact
     9421 cggccaacac cgacatcgcc acagccgagg atgcgctgtc cacccgcgtg gattacatca
     9481 aggaacagtg tgccgattac agcagcctga atctggcatc cgaggccaag agcctgctgc
     9541 gcgtgaccga aagcgcccag cagtgcgccc gggatatctg gtacctcacc gaaaagcccg
     9601 acgaccttta ccccgctgca gaggacgtct taccgcactt cgtcgagaac cactacctga
     9661 ccgggctgct gcttctctcc cgggatggac agatcaccaa aagctactgc aaggaggagg
     9721 ccctgctgcc cctgttgaac aaggtgctga cgaccgattc ccttttggat gtggccgacc
     9781 acccgaaaaa gacctacgct gtgcgcctcg cctgcgggga tgactccttt ttggatattg
     9841 ccgcctcggc ccgtgagggg gccgactgca tcgttgttgc gttctaccac acccccgccg
     9901 agtacgtcca cacttacaaa ttgtcgtacc agcatttggt ttccggcttc catatcgcta
     9961 cggacggcac gctggccgtg gccagcggca ataaaatcat tgccgccaac gatgaaagcc
    10021 tgctggggct ttccaccgac gaggtcgcac cgctgcagct catcaagcag cgcggcgtct
    10081 atgacaggat ggtctatgta cgtgacaccg gcatgggcac cagcggctac ttcggctaca
    10141 ccgagaaggg ccgcgactgc tacgtctacg cctttttgcc ggaggccgcc gtctttacca
    10201 caacgcccaa gaacatcatc tttgtagtga tttcctacac cctcattatt cttttgattg
    10261 aactggtgcg ccgccgcacg atgcactcct atcaggagga aaagctcctg caggagaaag
    10321 cctaccagaa atcgctggaa aaggccgccc gcaaggctga ggccgccaac gttgccaaga
    10381 ccgagttctt gcagcggatg agccatgaca tccgcacgcc catcaacggc atccggggca
    10441 tggtggagat tggcgaccac tacgccgacg atatgaccaa acaggccgag tgccgccgca
    10501 aaatctggga cgcctcgggc tttttgctgg agctcgtcaa tgaagttttg gacatgggca
    10561 agctggaatc cggtgaggtc gtgctcgaac accggccttt caacatggtc aagctgctga
    10621 ccgggttgca ggatgtgctg gaaaagcagg ccgccgggcg cggcatccgc atactctgcg
    10681 acgacatcga cctgccccac cccgatctca tcggcagccc gatccacgta aagcggctga
    10741 tgatgaacat tatgagcaac gccgtcaagt acaacaagga caacggcacc atcactatga
    10801 ccctgcggga ggaacgctgc gacggcaaga ccgcctgggt caagttcacc tgcgccgaca
    10861 ccggcatcgg catgagcgag gaattccaga agcacatctt tgagccgttc acccaggagg
    10921 ttctggacgc ccgctcgacc tacggcggta ccgggctggg tatgcccatc gccaagagcc
    10981 tggtggacaa gatgggcggc accatcacgt ttgagagccg gcagggcgtc ggtacgacct
    11041 actacatcac gctgccgctg gaaatcgacc ccaacgccgc gccccagccc gagacggaaa
    11101 agcccgccga cgacgccacc ttgcagggtg tgaacatcct gctggccgag gacaacgacc
    11161 tgaacctgga aattgcccag ttcctattgg aaaacgccgg ggccaccgtc accgctgccc
    11221 gcaatgggca ggaggccgtg gacatttttg ccgcatcccg gcccggtgag tttgacgcga
    11281 ttttaatgga tgtgatgatg ccggtgatgg acggctacac agctacgcga accattcgca
    11341 ggctggaccg cccagacgcc gggcgcatcc cgattttagc tacgactgcc aacgcctttg
    11401 ccgaggaccg ccgccgtgcc tgcgaggccg gtatgaacga gcacctgacc aagcccctgg
    11461 aaaccgagct ggtcataaaa acgctggcga aatatcgcaa ataatccaaa acgcctgtgc
    11521 accgcgcttt gctatgcggt acacaggcgt ttttaggtaa ggctttcccc tcgggggaag
    11581 gctgcagttt ttattttcct aaggtccttc gtatcatcag ccgcaggtta tccatcaggt
    11641 cttccacggt gcggtcgtgg tcagcggcat agcacaggcc catcaggccg cctacaacgg
    11701 cggaaagcag cgacatatag caatactcca tgccaagctc cggagcgatt tcccccgccg
    11761 cctgcagccg cgccagcagt tcacttacga tttctttcag ttcggccatg caggcgggca
    11821 tagcacccgt cctgcggggc attcccagcg tctcggcaat catcttgtag ctgcccatcc
    11881 ggctggcggc aatgtcctgt aaatagccgg taatgcgttc ttccagcccg gccagcgtcg
    11941 tacagtcggc gaacatggca cggacacgct cgttttcccg ggcaaagcag tactcggcca
    12001 agccctgcaa aatttcctct ttggaggaaa aatactcgta caccgtgccc ttgccgatac
    12061 cggcggcatc ggcaagctgc tgtattttca gcgcggcagg gctgacgccc tggccgataa
    12121 gctgcagcgc agcctcatac acggcggcct tgcgggcggc ttgctggtca ttctgcatcg
    12181 tggtccgcct ccttttcctc gttgggcgtc tcgttttccg ccggggcgtg ggccgcgcgg
    12241 ctcagatccg ggcggcgctt ttcttcggcg ggcgctttgg ccgcattctc ggcggcacgg
    12301 cgggcggcga attcggccgc aaactcagcg gcatcgtcgg gcaggtcatc catgccgtca
    12361 tcaccgacgt cgatattcac cggcggcttg cggtagaaca gatcatacag cacaggcacg
    12421 atgaacagcg tcatcagcgt ggcgtagctc agaccgccaa taatgacgat agccatgccg
    12481 gtgctcatct cgctggccat atcggtgccg aacagcatcg tgaccatggc aagcacggtg
    12541 gtcagcgtcg tcatcaggat cgggcgcata cgggtccggc cggtagcgac aagggcatcg
    12601 ctgcgctcca acccgccgat gcgcagctgg ttggcgtagt caacgaacac aattccgttg
    12661 ttgacgacga cacccatcaa taccagaaag cccatcagcg aaatgagcga cagctgctcg
    12721 ccggtggcca tcaggcccag cataccgccg gtgaaggcca gcggaatcgt gaacagaacg
    12781 atgaacggcg acagcaggct ttggaactgc gcgaccatga caaagtagac gaatagcagc
    12841 gccagcgcca gcatcttcgc catctggccc agcatctcgg tgacctgggt cgtctcgccc
    12901 gccagctcgg cggtcacacc gtcgggcagc tgcaattcgg ccagcttggg ttccagctcg
    12961 cgggaaatca gcgttgtgtt gtacccttcc gccgtctcgc tggtgatgga cagctgacgg
    13021 gagccgttct cgcggctgat ctcagtgtag cccgccgtgg tggtacgggt tgcgaactcg
    13081 cccagggtgt gggtctcctt tacggtgttg ccatcctcgt cggtggtggt cgtctcaaac
    13141 tccatgttga agatatcgtc cagcgtcggc gtgtcggtct tgtccacaat ggtcacggtg
    13201 tagctggtgc cgcccgcttt caaggtcgtg gcggtggtgt cggtggtcag cttggcggcg
    13261 atctgctggt agacctgcgc cacagtcagg cccatgcgca tggccttgtc cttgtcgatg
    13321 gtgatgttca cctcggcatc gccggcgtcc tggccatcgg tagggttgac gatgccgtcg
    13381 atgccgttca tggcctcgat gacctgtttg cccgcggcgc tcagatcatc caggtcgttg
    13441 ccgtagagat cgacctcgat gccggagccg gacagggcgc tcatatccat catgccgttg
    13501 gaattcacgt cgatctcaca gttcagatcg gccgtcttgt cgtagatttc ctgctccacg
    13561 accttggcct tcggggcctt gtcgcccagc aggacatagt aagtaaacgc cgtgttgtcg
    13621 gtagcggcgc tgctgcccgc catcatgacc atgctgttgg ggctgccgga cattgcgccc
    13681 acggtgtcca cgccgtccac cgtcatgatg gcgtccatga cgctgtccgc cgttgtgaag
    13741 gcatcctcct tggcggtgtc gtcgggcacg ttgaccgtga cggtcagctg gttgctgctc
    13801 atatccggga tcatgacaac gcccatcgtc gccacgcgcc agatggagac acccagcagc
    13861 acgattgcca gcgtcagcgg cacagccttg cgacgcaggc agaagcgcag cagtttttca
    13921 tagccgttca gcgccttgtc gaaccaaggg tgcttgatat ccttctgctt tttcagcacc
    13981 gtggaacccg cgcagggcac gacggtcagc gccacgataa gggacgccag caaactgaag
    14041 gtaatcgtcc acgccatatc gctgagcagt tcgcgcacca tgccggtggt gaacaccagc
    14101 ggcaggaata cgcagatggt cgtcagcgta ctggacacga tggacccggc cacctgccgt
    14161 gcgccctgca ccgacgcgcg gggggcaggc acgccgcgcc cgcgcaggcg gtagatgttc
    14221 tcgatgacaa cgaccgagtt atcgacaagc ataccgacgc ccagcgccag gccggacagg
    14281 gacagaatat taaacgtgat gccggtaaag tacatccata cgatggcaaa cagcacgctc
    14341 agcggcatac tgatggcgac aacgatggtg gggcgcaggt ctttcaggaa cagcgccagc
    14401 acgatgatcg cgaggatcgc gccctcgata aggttcgaga gcacgctgtt gacgataagc
    14461 ttgatgtagt cgccctggtc catgatgggc gtcaggtgca gaccctcggt ctcggccatc
    14521 atttcttcca tggcggcgtt ggccgcgttg gagacggtgg aggtggaggc cgtcgagctc
    14581 ttgtagatag acagcagtac cgcgcggttt ttgtccacct tggcgtaggc gtcgtcggcg
    14641 ttgtcggtca tttcgacctc ggccacgtcg gccaggcgca catccccgat gccgtcaata
    14701 ttgcacagca ccatgttttt cagctcgtcg gtgtcggcaa aggcgtcgcc cactttgata
    14761 atgtagcggt tgtcgtcccc tgcgccgccg tcgatataac cggcgggcat cgagaagttc
    14821 tgggccgcaa tcagctgggt cagtgtcgcc atgttcagca gctgatccag gttcgcgttt
    14881 ttcagcgctt cctcgcgggc ggagtcgtac tcctcctgcg cggcttccag ctgggtctta
    14941 ccggcttcca gctgggtacg gccgctggat atctggaact cgcccagctg cagctgggcc
    15001 tcgctgccgc tcaaaagcag ctgcacggcc gcatcggcca tggcggggtc cacggcgggg
    15061 tcacccaggc ttgccagcgc ggattccatc ttggcgcgtt ggtcgttgag ctgggtcagt
    15121 atttcctggg ctttggtcag ctggtcattc agcgcctgca cggtctcgtt caaggtctgc
    15181 tgcagcggct tcatgccctc gttcagcccg gcctccgcct gctggcgggc ctgcagcaat
    15241 accgtcgcgt tattcagcgc cgtgctggtc gtggacaggg tcgtcaggca gccgttcaac
    15301 tcggtcaggc gggcgcggcg gggggcattg tcgtccagaa ttttctggta acccgcctgc
    15361 tcggcgcgct tggcgtcgat ttgcgcctgg atcgcctgct gctgggcctc gtcaccctcg
    15421 gcagcggcct tgtcatcctc cagcccggcg atttccgcat ccaacgcctg gatgcgcccg
    15481 gtcagcgccg tgtactgggc gtcaaggtag gccgcgctct gggccacgtc catgccggat
    15541 tccagcaggt tttccgcggc agcggtgctg ttgatgttct gtgtggcgcg ctcgatggcg
    15601 ttgaccattg cgcccatctt ggccgtgctg cccttggcct cggccaggtt ggcgggcagc
    15661 tcattggcgt tatattggga gtcccacgca gccaaaatct gcttgcagct ggcaaacagc
    15721 gcgtcgtcga tgggcagcgt gacctccggc agggtggtgt cggcgttctt ggcgtcctcg
    15781 agcttgcggt tcgcgtcgtc gatatcggtt ttcaaatcgg cgattttctt ttccagctca
    15841 gggatttgtt tgtccagctc ggtgatggcg tcccgcagct gggtcgtcac atcggctttc
    15901 tgggtgttca gctcggattt gcccgcgtcc agctgggcct gggcgtcgtc gagttctttc
    15961 aggccgtcgt tgattttctt cttgttatcg tccagttctt ttttcgcgtc ggccaggcgg
    16021 tcactgacct tgaccagcag tttgtcattc actgcgtcga ttttatcctg gtccagcgtg
    16081 atttgcacag acttttccac aagtcctgtg gttgaaacac ttgccacgcc gtccacgcgt
    16141 tccagctggg gcagcacgtt ctcgctgaca taatcggaca actcgtagat atccatgcca
    16201 tcataatcga ccgccacata ctgggtcgcc atcatgtcag ggctgatttc cataagcgtc
    16261 ggagtggacg ccatatccgg aagggcgtcg cccagctggt tcacggccgt gctggccttg
    16321 accatggcgc tgtcc
//
LOCUS       DBMDSQ010000015        15933 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_1170_length_15933_cov_2.536635, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000015 DBMDSQ010000000
VERSION     DBMDSQ010000015.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 15933)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 15933)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15933
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1170_length_15933_cov_2.536635"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(204..1595)
                     /gene="mnmE"
                     /locus_tag="ACZPAX_01165"
     CDS             complement(204..1595)
                     /gene="mnmE"
                     /locus_tag="ACZPAX_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005929161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HIR1112538.1"
                     /translation="MEHLHTTICALATPPGEGGIATVRVSGPDAYGIVGKIFAPVRQG
                     KSVADAKGYTAMLGHYLLHGAEMDECVALFFRAPHSYTGEDVIELSVHGGTAMADGLL
                     EALITAGCAPAGPGEFTRRALENGRMSLTQAEAVMEVIAANGRQGAALAKSALDGRLA
                     KRIGCIQTALQTLNAHLTAWVDYPEEDVPELSDEAFVQSLTEQKAELDALINGYSAGA
                     VLRHGVDCVLLGRPNVGKSTLLNLLAGFDRAIVTPVAGTTRDIVEQAVQLGSVRLNLF
                     DTAGVREVGVDGDAIEAEGIRRSWKKLDEAGLVLAVFDAAQPLSEDDLELARKCRGRP
                     AIAVLNKQDLAGKQDVPRGTLEPYFKKIVPMCAKDAVGLQALTDAVADLLGTAQLDPG
                     AAQLCSARQLSAATRARDALAEAICARQDGFGLDAAAVCLTDALQALCDLTGEDATEA
                     TIDEVFETFCVGK"
     gene            complement(1708..4284)
                     /gene="gyrA"
                     /locus_tag="ACZPAX_01170"
     CDS             complement(1708..4284)
                     /gene="gyrA"
                     /locus_tag="ACZPAX_01170"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015537092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HIR1112539.1"
                     /translation="MEENIIMVPGSGTKVIVRDVKQEIETAFLDYSMSVIVARALPDV
                     RDGLKPVHRRILYTMHERGNDPQHPYRKSADTVGAVLGSYHPHGDASVYDAMVRLAQD
                     FSLRYPLVDGQGNFGSVDGDPPAAYRYTEARMSKMACEMLTDIEKETIDWDPNFDETK
                     KEPHVLPSRFPNLLVNGSQGIAVGMATNIPPHNLREIVNGMVALMDDPEIDLAGLMEY
                     VKGPDFPTGGIIMGRSGIRAAYATGRGKITLRGRAEIVEKKNGRYEILISEIPYMVNK
                     TRLIESIADLVKDKRIEGISDLNDESSSRTGMKIVIEIKKDANPQVVLNQLYRYTQLQ
                     DTVGVIMLALDDGVPKIMRLKTMMERYIEFQMQVIRRRTAFDLRKAKEREHILEGLHK
                     AVDIVDEIIATIRACKGGFAEARQAVMDNFGFDEPQADAIVKLQLGRLAGLEILKIEQ
                     ELGELREAIADYEDILANDEHVKRIVKTDLTTLADKYGDERRTSIEAVSGEVDIEDLI
                     PEETCVFTLTHEGYIKRTTLDTYQAQNRGGRGVQGMTQKDEDFTEEMYVGSTHDYMLF
                     LTNQGRVYRLKGYQVPEGSRTAKGSHIVNLLQLQEGEKVTLMLQQKADVDEDNTYATM
                     ITKQGLIKRTPLSHFRNIRKAGLIAIALNEGDSLVWSHLTKGDDEIIVATHDGAAIHF
                     TEDDARAMGRTGHGVRVIKLREGDYVVGAGVCRPGATVLTISEEGKGRRSRIDDYRIT
                     KRGGLGIRNYSNGNVAGIKIVDDTDDLILISQNGILIRIHASDINIQSRYGSGVRVMR
                     LVEDDKVAVVARVDRDNEAETAKIEDTGETDPTPEELAAIEAEELAQEAAEDAADTND
                     EE"
     gene            complement(4406..4867)
                     /locus_tag="ACZPAX_01175"
     CDS             complement(4406..4867)
                     /locus_tag="ACZPAX_01175"
                     /inference="COORDINATES: protein motif:HMM:NF013921.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HIR1112540.1"
                     /translation="MDYTILCPRKNPRLQNYDYRRENYYFVTVCAHHKQCVFGAAGKP
                     NVVGQMAAQGIKDISRHFPGVGVDEYVVMPNHVHMILVFPQEQCNLSAVVGSYKSYVS
                     KMFHVEHPGVTLWQRSFYDHIIRDENAYCEIKRYIQENPLKWDLDEYNPHA"
     gene            complement(4951..6933)
                     /gene="gyrB"
                     /locus_tag="ACZPAX_01180"
     CDS             complement(4951..6933)
                     /gene="gyrB"
                     /locus_tag="ACZPAX_01180"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015537091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HIR1112541.1"
                     /translation="MAEEIITETNRETATDHAYGGAQIQVLEGLEAVRKRPGMYIGST
                     GPSGLHHLVYEIVDNAIDEALAGYCTHIEVTIKPGNIIEVSDNGRGIPVDIQPKLGIP
                     AVTVVYTVLHAGGKFGGEDSGYKVAGGLHGVGASVVNALSEWLTVRVRRDGVEYEQSF
                     KRGKPDGDLKKIGAAASTGTTVTFKPDPEMFQETTLYRYETLLKRLREEAFLNAGVKI
                     TLTDERPDADRPQDEQNGEMRAETMCYEGGIRSFVSYLCERRDLTPLHPQVMYMKGEG
                     QGAVAEVALQYYDNSYNELLLSFANNVHTPEGGTHEEGFKLALTRVLNEYGRAKGILK
                     DKDENLSGPDAREGIIAVVSVKLQEAQFEGQTKAKLGNTFIKGLVNNVVYKALTEFFE
                     ENPAVAKAILEKATQAARARAAAKKARELVRRKSALESNRMPGKLADCHEKDPAKTEL
                     FIVEGDSAGGSAKMGRDSNIQAILPLWGKMLNVEKARADRIYGNDKLMPVVTALGTGI
                     GDEFDISKVRYHKIFIMADADVDGSHICTLMLTFFFRYMRPLIDNGYVYVAQPPLFKL
                     QKGQTVKYAYNDEEMKVLSAEMPGAKVNRYKGLGEMNPDQLWETTMNPDNRVIVQIKI
                     EDAERADEAFSILMGEQVEPRRQFIEKNAKYANLDV"
     gene            complement(7088..7348)
                     /gene="remB"
                     /locus_tag="ACZPAX_01185"
     CDS             complement(7088..7348)
                     /gene="remB"
                     /locus_tag="ACZPAX_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007048461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular matrix regulator RemB"
                     /protein_id="HIR1112542.1"
                     /translation="MYFHAGQDFVLNTRDVIGVFDLDTAGASRRTEEYFRHAENEGAV
                     VDLCAPGTLPKSFMVTDFPDETVYISQLSPAALKKRAEKLEF"
     gene            complement(7425..8519)
                     /gene="recF"
                     /locus_tag="ACZPAX_01190"
     CDS             complement(7425..8519)
                     /gene="recF"
                     /locus_tag="ACZPAX_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005929121.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HIR1112543.1"
                     /translation="MRLTHLELTNHRNIVHAELNPDPHLTVLCGPNGQGKTNLLEAVW
                     LLTGGKSFRGSKDAELIQRGCDFSVIEGTFETQDVEKSIRLTVGSKGSQRPGRTGKLN
                     GADIGRAAALAGNFQAVVFEPDLLSLVKGGPDGRRRFLDSALCQVYRPYLVALRRYMR
                     LTAQKNALLKSYDITPNGGVLLDTYDEQLAEYGALIMDHRCKFLVAAAPIAAQNYKDI
                     SHGAETLELHYQMCTPAPTADALLEKLHAMRPAELRAGFCLAGPHREDLEILLDGQPA
                     RVYGSQGQQRSCVLAMKLAEATVVGDLFGEHPVLLLDDVLSELDDERQTYLLTRMGEH
                     QTIVTTCDTAAFARTNGKIVMVKGGTVAEP"
     gene            complement(8519..8734)
                     /locus_tag="ACZPAX_01195"
     CDS             complement(8519..8734)
                     /locus_tag="ACZPAX_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005924246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HIR1112544.1"
                     /translation="MEKIRINTEFIKLDALLKFAGLCETGGEAKELIQGGAVKVNGEV
                     CTMRGKKCRAGDTVELDGQTVQVAEGA"
     gene            complement(8737..9846)
                     /gene="dnaN"
                     /locus_tag="ACZPAX_01200"
     CDS             complement(8737..9846)
                     /gene="dnaN"
                     /locus_tag="ACZPAX_01200"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007048464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HIR1112545.1"
                     /translation="MKFECEKTLLASAIDGVSRAITNRAAIPVLEGIYMKAEGFNLTL
                     TGYDMEMGITTTIECNVLVPGETVLDAKLLSAMINRMPAGDVCIELNDEGQAKISGGV
                     AEFEIPALNASDYPSLPVTGADNTMTVKCGMLREMIEKTIYAVSQDDKKPAHTGELFV
                     IEPGSLTIVALDGYRLAIIQRDVECTRDIRIIIPAKTLQELLKIMGGADDDVKIDANR
                     RYVVFTSNGYTIMSRLIEGDFLNYESVIPKDKKTRITVDCKTFIDTIERASLIITERL
                     KNPLRISFGEDRVTVRCQTPLGKVVDEFAPVQMEGDAVEIGFNNRYLLDALRYSKCEK
                     MVLEINGPLSPVKLLPEDGKDFIYLVLPVRFKNEG"
     gene            complement(10345..11667)
                     /gene="dnaA"
                     /locus_tag="ACZPAX_01205"
     CDS             complement(10345..11667)
                     /gene="dnaA"
                     /locus_tag="ACZPAX_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007048467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HIR1112546.1"
                     /translation="MDSINEVLEAVKAYCKEKTSDATYAFYIKDIKAVSFENSNTITL
                     ELRSEFVMKIVSDRYTALLKEAFKAILGFDVEILYVAAPPEDGEKADGSADQRDEKSL
                     PSGRYDFTFENFIKGPSNQFAFAAAQAVAANPSGAYNPLFIYGPSGLGKTHLLNAIQF
                     EIKKNHPDFNIVYVDCEMFTNETISAIKTATMEQFRQKYRKADVLLIDDIQFLAGKES
                     TQEEFFHTFNTLHNDGRQIVIASDRPAKEIKSLEERLRTRFEWGLTADIQPPDFETRV
                     AIVKRKAELLNLDLPNDVAEYIANHLKQNIRQLEGAVKKLNAYYMLEGIEPCIGVTTT
                     AIKDTLNDSQPIPVTIEKILNEVARTYNVMPADIRGKKRNANVSAARQMTMYIIREVT
                     GMSMEAIGQEFQRDHSTVVYSIKTMEENINRDQHLKEMCSDIMKNVRT"
     gene            12087..12221
                     /gene="rpmH"
                     /locus_tag="ACZPAX_01210"
     CDS             12087..12221
                     /gene="rpmH"
                     /locus_tag="ACZPAX_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005924258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HIR1112547.1"
                     /translation="MKRTFQPKKRQRSRVHGFLNRMATKNGRKVIARRRAKGRKSLTV
                     "
     gene            12512..12874
                     /gene="rnpA"
                     /locus_tag="ACZPAX_01215"
     CDS             12512..12874
                     /gene="rnpA"
                     /locus_tag="ACZPAX_01215"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007048470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HIR1112548.1"
                     /translation="MRYRTLNKNWQFNRVYGRGKSYVHPHCVLYVAKNRVGYTRIGLT
                     ATKKVGHAVQRNRARRVMRAALAEHLSQNIGGYDIILVARGQTPRLKSTQLSKTLGKL
                     FTKAGLPDKAAPAVPAAE"
     gene            12871..13131
                     /gene="yidD"
                     /locus_tag="ACZPAX_01220"
     CDS             12871..13131
                     /gene="yidD"
                     /locus_tag="ACZPAX_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005929149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HIR1112549.1"
                     /translation="MITRFLIKLLRGYKRYISPHLGHHCRFVPTCSEYAMQALATHGL
                     VKGLLLTTWRLLRCQPFAKFGYDPVPPKGRWVSPERRLTRSR"
     gene            13365..14387
                     /locus_tag="ACZPAX_01225"
     CDS             13365..14387
                     /locus_tag="ACZPAX_01225"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HIR1112550.1"
                     /translation="MNFMYFLAILIGPLVKILYSFIGNYAVTMVVATLLLKLLLFPLS
                     IHQQKSSAKMAVFQPLMNEIQQKYKNDPQKQQEELMKLQQEHGFNPMGGCLPMLLTML
                     VLFGFLGVVYYPVHYIFGVDNTAVKAACEAIGLATTNASTMQTALIQAIHNGAVIDPS
                     IIPASVVSEIQNFNTSFFGMDMCDVPGFRLVPIAIFPAIAAVTMFISYFVTQKLSGMD
                     AQMQGSMKVMMLVMNLMFVTFCFNAPVGFSLYYGVSNLLQIGQSYITYKIYSPEKFKA
                     QYEAELAAKRAERKKKRTVTVEQNGKQVEKEVTLGEANKLRLERARQLEAELYKDERT
                     TPLNKD"
     gene            14553..15704
                     /gene="jag"
                     /locus_tag="ACZPAX_01230"
     CDS             14553..15704
                     /gene="jag"
                     /locus_tag="ACZPAX_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015565054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HIR1112551.1"
                     /translation="MRSMEMSAKTVEAAVQAACEALGVDRDDINVSYEVLEFPTRKLF
                     KTIPAKVLVKVEEPEAAPAPAAEEKPAEVSTKPAEVVEISDETAPEIEKAVVEDMVEA
                     AELAPVAEDDVEIPLDIAADPRLQAAVDYLTPIFNLMGVENFSFTAVKKGAATILKVS
                     GEHMGALIGRRGETMESLSYLASLVVNRMEGPYIKLGLDVGGYRNKREDDLSALAKRI
                     ADRVIRTGCYYEMEPMNPYERHIIHTAIADIEGVRSESKGEGPARHVVLYSTDPNASN
                     LPDRDNARNQRGGRRDGGRDPRRDGNRGYRGNGPRGPRSGRDGRDGGRGGYRGNRGDR
                     GPRYGGPRSSVPARDFADRPRDPDAKPMAPKTTERIHDGDDFAFGKIEF"
BASE COUNT         3131 a         4762 c         4483 g         3557 t
ORIGIN      
        1 cggccacctt cccccaaagg gggaaggctc tccacaaaag gcttctcccc ctgggagaag
       61 ctgccgcccg caggcggctg atgaggggcg gcttaactct attgtccctt taccgagttg
      121 ccgccggaaa ctcgcccctc attcgacctc ggtcggagcc tcggccacct tcccccgcag
      181 ggggaaggct ccttattatt tacttacttg cccacgcaga atgtttcaaa cacttcatca
      241 atcgttgcct ctgtcgcgtc ttcaccggtc aaatcgcaca acgcctgcag ggcgtccgtc
      301 aggcagacgg cggcggcgtc cagcccaaag ccgtcctgcc ttgcgcagat ggcttcggcc
      361 agtgcatccc gcgcgcgggt cgcggctgaa agctgccgcg cgctgcaaag ctgcgctgca
      421 cccgggtcca gctgtgccgt gcctaacaga tctgccacgg cgtccgtcaa agcctgcaag
      481 cccacggcgt ctttggcgca catggggacg attttcttaa aatacggctc caatgttcca
      541 cgtggaacat cctgctttcc cgccaggtcc tgtttattca gcacggcaat ggccgggcgg
      601 ccccggcatt tgcgggccag ctccaaatca tcttcggaga gcggctgcgc ggcatcgaac
      661 accgccagca ccagcccggc ttcgtccagc tttttccagc tgcggcggat gccttcggcc
      721 tctatcgcgt caccatcaac accaacctcc cgcacaccgg cggtgtcaaa caggttcaac
      781 cgcacgctgc ccagctgcac ggcctgctcg acaatatccc gggtcgtgcc tgccaccggc
      841 gtgacgatgg cccggtcgaa cccggcaagc aggttcagca gcgtgctttt gccgacattg
      901 ggtcgcccca gtaacacgca gtccacgccg tggcgcagca ctgcgcccgc actgtaccca
      961 ttgatcagcg cgtccaactc ggccttctgt tctgtcaagc tttgcacaaa cgcctcgtcg
     1021 gacagttcgg ggacatcctc ctcggggtaa tcgacccagg cggtcaggtg ggcgttcagc
     1081 gtttgcagtg cagtctggat gcagccaatg cgttttgcca gccgcccgtc cagtgcggat
     1141 tttgccagcg ccgccccctg ccggccgttc gccgcgataa cttccatcac ggcctcggcc
     1201 tgggtcagcg acatacgccc attttccagt gcgcggcggg tgaactcacc ggggcctgca
     1261 ggcgcacacc ccgccgtgat cagcgcttcc agcaagccgt ccgccatagc cgtgccgccg
     1321 tggaccgaca actctatcac atcctcgccg gtgtaggagt gcggtgcgcg gaaaaacagt
     1381 gccacgcact cgtccatctc cgcaccgtgc agcaggtaat ggcccagcat ggcggtgtag
     1441 cccttggcgt cggcaacgct cttgccctgc cgcaccggcg caaaaatctt gccgacgatg
     1501 ccgtaggcgt ccggcccgga gacgcggacg gtggcaatgc cgccctcccc tggcggcgtc
     1561 gccagggcac agatggtcgt atgcagatgt tccatgtggt actcctttat ttttctttta
     1621 ccaaaaagga gaggccaacc cggcctcccc ttcacggttt gctattttgg agtttctttg
     1681 cttactttct ttgcaaagaa agtagggtta ttcttcgtcg ttggtatcgg ctgcgtcctc
     1741 tgcggcctcc tgggcaagct cctccgcctc aatggctgcc agctcctcag gggtcgggtc
     1801 agtctcgccg gtatcctcga ttttcgcggt ctcggcttcg ttgtcgcggt cgacacgggc
     1861 aaccacggcc accttgtcat cctcgaccag gcgcatgacg cggacaccgc tgccgtagcg
     1921 gctctggatg ttgatgtccg acgcatggat gcggatcagg atgccgttct ggctgatcaa
     1981 aattagatca tcggtgtcgt ccacgatctt gatgcccgcc acattgccgt tggagtagtt
     2041 gcggatgccc aagccgccgc gcttcgtgat gcggtaatcg tcgatgcggc tgcggcggcc
     2101 cttgccttct tcgctgatgg tcagcacagt ggcacccggg cggcagacgc cggcgccgac
     2161 gacatagtcg ccctcgcgca gtttgataac gcggacgccg tggccggtac ggcccatggc
     2221 gcgggcatca tcctcggtaa agtggatggc cgcgccgtcg tgggtggcga caataatttc
     2281 atcgtcgccc ttcgtcaggt ggctccaaac caagctgtcg ccctcattca gggcaatggc
     2341 aatcaggccc gccttgcgga tgttgcggaa gtgggacagc ggtgtgcgct tgataagacc
     2401 ctgcttcgtg atcatcgtcg cgtaggtgtt gtcctcgtcc acgtcggcct tctgctgcag
     2461 catcagcgtg accttctcgc cctcctgcag ctgcagcagg ttcacgatat ggctgccctt
     2521 ggcggtgcgg ctgccctcgg gtacctggta gcccttcagg cggtagacac ggccctgatt
     2581 cgtcaggaac agcatatagt catgcgttga gccgacatac atttcctcgg tgaagtcctc
     2641 gtccttctgg gtcatgccct gcacaccgcg gccgccgcgg ttctgtgcct ggtaggtatc
     2701 cagcgtggtg cgcttgatat acccctcgtg ggtcagcgta aagacgcagg tttcctcggg
     2761 gatcagatcc tcgatgtcga cctcgccgga cacagcctcg atggaagtgc ggcgctcgtc
     2821 gccgtacttg tcggccagcg tggtcaggtc ggtcttaacg atgcgcttga cgtgctcgtc
     2881 attggcgagg atatcctcat aatcggcgat ggcctcgcgc agctcgccca actcctgctc
     2941 gattttcagg atttccagac cggccaagcg gcccaactgc agcttgacga ttgcgtccgc
     3001 ctgcggctcg tcgaagccga agttatccat gacagcctgg cgtgcctcgg caaagccgcc
     3061 cttgcaggcg cggatggtcg cgataatttc atcgacgatg tcgacggcct tgtgtaggcc
     3121 ttcaaggatg tgctcgcgct ccttcgcctt gcgcaaatca aaagcggttc tgcggcggat
     3181 gacctgcatc tggaactcga tgtagcgctc catcatggtc ttgagtctca tgattttggg
     3241 cacaccgtcg tccagcgcca gcataatgac acccacggtg tcctgcagct gggtgtagcg
     3301 gtagagctgg ttcagcacga cctgggggtt cgcgtccttc ttgatttcaa taacgatctt
     3361 catgccggta cggctggagg attcgtcgtt caggtcgctg atgccctcaa tgcgcttatc
     3421 cttcacaagg tccgcaatgg actcgatgag gcgggtcttg ttgaccatgt agggaatttc
     3481 gctgattagg atttcatagc ggccgttctt cttctcgacg atttcggcgc ggccgcgcag
     3541 ggtgattttg ccgcggcccg tggcgtaggc tgcgcggatg ccgctgcgtc ccatgatgat
     3601 gccgccggtc gggaagtcgg gacctttgac gtattccatc agcccggcca ggtcgatttc
     3661 ggggtcgtcc atcagggcga ccatgccgtt gacgatctcg cgcaggttgt ggggcgggat
     3721 gttcgtagcc ataccgaccg caatgccctg gctgccgttg accagcaggt tcgggaagcg
     3781 gctgggcagg acgtggggtt ccttcttggt ttcatcaaag ttgggatccc agtcgatggt
     3841 ctccttctcg atatccgtca gcatctcgca ggccatcttg gacatacggg cctcggtgta
     3901 acggtaagca gccgggggat cgccgtcaac ggaaccgaag ttgccctgac catcaaccag
     3961 cgggtagcgc aggctgaaat cctgtgccag acggaccatg gcatcataga ccgacgcatc
     4021 gccgtggggg tggtacgagc caagcaccgc accgacggta tcggcagatt tgcggtaggg
     4081 gtgctgcggg tcgttgccgc gctcgtgcat cgtgtacagg atgcggcggt ggacgggttt
     4141 caggccgtcg cgcacatcgg gcaaagcgcg cgccacgata acggacatcg agtagtccag
     4201 gaacgccgtc tcgatctcct gtttcacatc gcgcacaatg accttagtac cggaccccgg
     4261 caccatgatg atgttttctt ccattgtatc accttaactc aatcttactt gaacttacta
     4321 attttactgc accgtagggg ccgggcatgc ccggccctca actttgcgtt attgcccaca
     4381 aacggatcgt tgcttgtacg ctgcgctatg cgtgtgggtt atattcatcc aaatcccatt
     4441 tcaacggatt ttcctgaata tatcttttaa tttcacaata cgcgttttca tcacgaataa
     4501 tatgatcata aaatgaacgc tgccacaatg tcacacccgg atgttccaca tggaacattt
     4561 tggacacata cgacttgtag cttcccacta cggcggacaa attacattgt tcctgtggaa
     4621 aaaccaatat catatgaaca tgatttggca ttaccacata ttcatccaca cccacaccgg
     4681 gaaaatgtct ggaaatatcc tttattcctt gtgcggccat ttgtccgacg acatttggtt
     4741 ttcctgccgc gccaaacaca cattgtttat gatgcgcaca tacggtcaca aaataataat
     4801 tttctcttct ataatcataa ttttgcaaac ggggattttt gcgcgggcat aaaatcgtat
     4861 aatccatgat tcatacacca taaacggggc ggtggttttt atgcggcggg ccgggcatgc
     4921 ccggccccta cacgggcggc gtatgatgta tcacacatcc aaattcgcat acttggcgtt
     4981 cttttcaatg aactggcggc ggggttcgac ctgctcaccc atcaggatgc tgaatgcttc
     5041 gtcggcgcgc tcggcgtctt caattttaat ttggacaatg acgcggttat cggggttcat
     5101 ggtggtctcc cacagctggt cggggttcat ctcgcccaag cctttgtagc ggttgacctt
     5161 cgcgccgggc atttcggcgc tcaacacctt catttcttcg tcgttgtagg cgtacttgac
     5221 ggtctgaccc ttctgcagct tgaacagcgg cggctgggcg acatagacat agccgttgtc
     5281 gatcagcggg cgcatatagc ggaagaagaa ggtcagcatc agtgtgcaga tgtgcgaacc
     5341 gtcgacatcc gcatcggcca tgatgaagat cttgtggtag cgcactttac tgatatcaaa
     5401 ctcgtcgcca atgccggtgc ccagcgcggt cacaacaggc atcagtttat cgttgccgta
     5461 gatgcggtcg gcgcgggcct tttcgacgtt cagcatcttg ccccacaaag gcaggatggc
     5521 ctgaatgttg gagtctcggc ccatcttggc cgagccgcct gcagaatcgc cctccacgat
     5581 gaagagttcg gtcttggcgg ggtctttttc gtggcagtca gccagcttgc cgggcatacg
     5641 gttggattcc agcgcgctct tgcggcggac cagctcgcgg gcctttttgg cggcggcgcg
     5701 ggcgcgggca gcctgggtgg ccttctccaa aattgccttg gcaacggcgg ggttttcctc
     5761 aaagaactct gtcagcgcct tgtagacgac gttgttgacc aggcccttga tgaaggtgtt
     5821 gcccagcttt gccttggtct ggccctcaaa ctgggcctcc tgcagcttga cgctgacgac
     5881 ggcgatgatg ccctcgcggg cgtcggggcc ggacaggttc tcgtccttat ccttcaaaat
     5941 ccccttggcg cggccgtact cgttcagcac gcgggtcagg gccagcttga agccttcctc
     6001 gtgggtgccg ccctcggggg tatggacgtt gttggcaaag ctcaacagca gctcgttata
     6061 gctgttgtcg tagtattgca gggcgacctc ggccacagcg ccctggccct cgcccttcat
     6121 atacatgacc tgggggtgca gcggcgttaa gtcgcggcgc tcgcacaggt agctgacaaa
     6181 ggaacggata ccgccctcgt agcacattgt ctcggcgcgc atttcgccgt tctgctcatc
     6241 ctgcgggcgg tcggcgtcgg ggcgctcatc ggtcagggtg atcttgacac cggcgttcag
     6301 gaacgcttcc tcccgcaggc gcttcagcag cgtttcatag cggtagagcg tcgtttcctg
     6361 gaacatctcc gggtcgggct tgaaggtgac tgtcgttccg gtagaagccg ccgcgccgat
     6421 ttttttcagg tcgccgtcgg gcttgccgcg cttgaagctc tgctcatact ctaccccatc
     6481 gcggcgcacg cggacggtca gccactcaga caaagcgttg acgaccgacg cgccgacgcc
     6541 gtgcagaccg ccggcgacct tgtagccgga gtcctcgccg ccgaacttgc cgccggcgtg
     6601 cagcacagtg taaacgacgg tgacagccgg gatgcccagc ttgggctgga tatcgaccgg
     6661 gatgccgcgg ccattgtccg atacttcgat gatgttgccg ggcttgatgg tgacttcgat
     6721 gtgggtgcag tagcccgcca gcgcctcgtc gatggcgtta tcgacgatct catagacgag
     6781 gtggtgcaga ccggaaggcc cggtggagcc gatatacata ccggggcgct tgcgcacagc
     6841 ctccaggcct tccaggacct ggatctgcgc accgccgtag gcatggtcgg tagccgtctc
     6901 gcggttggtt tcggtgatga tttcttctgc catgaaatga tagcctcctg ttgtgggtgg
     6961 gtatctctta ttttgcaatg taggggccgg gcatgcccgg ccctccgcct tgcgttattg
     7021 cccttttatc gggcggtact ttttatgttg cgggccgggc atgcccggcc cctgcggtcg
     7081 tggttcttta aaactctaat ttttccgccc tcttcttcaa tgcggcgggc gaaagctggc
     7141 tgatatacac ggtctcatcg gggaaatcgg tgaccataaa acttttgggc agggtgccgg
     7201 gggcgcaaag gtccacgacc gcaccctcgt tctcggcgtg gcggaaatat tcttccgtcc
     7261 gccgcgacgc cccggctgtg tccagatcaa acaccccgat gacgtcccgg gtgttcagta
     7321 caaaatcctg ccctgcgtga aagtacattt cctgcacctc tctgtagggg ctgggtatgc
     7381 ccggccctcc actctgcgtc attgcccgtc agcgggcaac caccctacgg ttctgctacg
     7441 gttccgccct tgaccatcac aattttcccg ttggtgcgtg caaacgcggc ggtgtcgcag
     7501 gtcgtcacga tggtttggtg ctcgcccatg cgggtcagca ggtaggtctg gcgctcgtcg
     7561 tccagctcgc tcaaaacgtc atccagcaat agtaccggat gctcgccgaa caagtccccc
     7621 actaccgtgg cctcagccag cttcatagcc agtacgcagc tgcgctgctg gccctggctg
     7681 ccgtagaccc gggcgggctg accgtccagc agtatctcca agtcctcccg gtgcgggcct
     7741 gccagacaga accctgcccg cagctccgca gggcgcatgg cgtgcagctt ttccagcagc
     7801 gcgtcggcgg tgggggccgg tgtacacatc tggtaatgca gttccagcgt ctcggctccg
     7861 tgggagatat ccttataatt ctgcgcggca atgggcgcgg ccgccaccag gaatttgcag
     7921 cggtgatcca tgataagtgc gccgtactca gccagctgct catcgtaggt atccagcagc
     7981 acgccgccgt ttggcgtgat atcgtagctt ttcagcagtg catttttctg ggccgtcagc
     8041 cgcatatacc gccgcagcgc taccaggtac ggcctgtaca cctggcacag cgccgagtcc
     8101 aggaaccgcc gccgcccatc cggcccgcct ttgaccaggc tcaacaggtc cggctcaaac
     8161 acgacggctt gaaaattccc ggccagtgcc gccgcgcggc caatgtccgc gccgttcaat
     8221 ttgccggtgc ggccgggtcg ctggctgccc ttgctgccca cagtcaggcg gatggatttt
     8281 tccacgtcct gtgtctcaaa cgtcccctct atgaccgaga agtcacagcc gcgctggatg
     8341 agttcggcgt ccttcgaccc gcggaagctc ttgccgccgg tcagcagcca aacggcctcc
     8401 agcaggttcg tcttgccctg accgttgggg ccgcacagca cggtcaaatg cgggtccggg
     8461 tttagttctg catggacaat attgcgatgg ttcgttaatt ccaggtgcgt taagcgcatt
     8521 acgcgccctc cgcgacctgc acggtctggc cgtccagctc gacggtatcg cctgcgcggc
     8581 actttttgcc gcgcatcgtg cagacctcgc cgttgacctt gacggccccg ccctggatca
     8641 gctcctttgc ctcgccgcct gtctcgcaca ggcctgcgaa tttgagcagg gcgtccagtt
     8701 tgataaattc agtgttgata cgaatttttt ccataatcag ccctcgttct taaagcgcac
     8761 cggcaaaacc agatagataa aatccttgcc gtcctcgggc aggagcttga ccgggctcag
     8821 ggggccgttg atctccagca ccatcttctc gcatttggag tagcgcagcg cgtccagcag
     8881 gtagcggtta ttgaagccga tctccaccgc gtcgccctcc atctgcaccg gggcaaactc
     8941 gtccacgacc ttgcccagcg gcgtctggca gcgcacggtc acgcggtcct cgccaaagct
     9001 gatgcgcagc gggttcttca ggcgctcggt gataatcagc gacgcgcgct cgatcgtatc
     9061 gataaaagtc ttgcaatcca cggtgatacg cgttttctta tccttcggga taacgctctc
     9121 atagttgagg aagtcgccct cgatcaggcg gctcatgatg gtatagccgt tggaggtgaa
     9181 cacgacatag cggcggttgg cgtcgatctt cacatcgtcg tccgcgccgc ccatgatctt
     9241 cagcagttcc tgcagcgtct tggcggggat gatgatgcgg atatcgcggg tacactcaac
     9301 atcgcgctgg atgatggcca aacggtagcc gtccagggca acgatggtca agctgcctgg
     9361 ctcaatgacg aacaactcgc cggtgtgggc gggtttcttg tcgtcctggc tgacggcgta
     9421 gatggttttc tcgatcatct cgcgcagcat accgcacttg acggtcatgg tgttgtcggc
     9481 gccggtcaca ggcaggctgg ggtagtcgct ggcatttagg gcagggattt caaattctgc
     9541 cacaccaccg ctgatttttg cctggccttc gtcgttcagc tcaatgcaga catcgcctgc
     9601 gggcatacgg ttgatcatgg cgctgagcag ttttgcgtcc agtacggttt caccaggtac
     9661 aagtacgttg cattcaatgg tggtcgtgat gcccatttcc atatcgtagc cggtcaaggt
     9721 caggttaaag ccctcggctt tcatgtagat gccttccagc acagggatgg ccgcgcggtt
     9781 ggtgatcgcg cgggagacgc cgtcaatggc actggcaagc agggtctttt cgcattcaaa
     9841 tttcatggtt tcttctcttt ccggcttttt tggaaaaaag cctcggcaaa aaacgtaaaa
     9901 tttactctac aatttgtata gttttggttt tccacaaaat ggttgccgtt tggcccggtt
     9961 gtagagcagt tgtagcaggt tcctatcagc agaaactatt aactttggta gtagcagtag
    10021 taggggctgt tagtttggtg gaaaactccg cggactgtga taacggcggc aaaaaccggc
    10081 cgtgaattgt tttgaacagc ctgtggacat ccttgtggaa aactcaaaac ccgcgctttt
    10141 tcaacacgcg gcgtggagaa ctgtctgtta aaaactaaaa actctgtgga aaaagttact
    10201 ttctttgcaa agaaagtaac caaagaaact ccaaacattc aaaccgtaaa gttatcgccg
    10261 ataaaacgtt tatttttatt cagcgatccc tttgcgggaa caaatattga aagttctttg
    10321 cctactttct ttcaagaaag taggctaggt gcggacgttt ttcatgatgt cgctgcacat
    10381 ctctttcagg tgctggtcgc ggttgatgtt ttcctccatc gttttgatgg aatataccac
    10441 cgtggaatga tcgcgctgga actcctggcc gatggcctcc atgctcatgc cggtcacttc
    10501 ccggataatg tacatcgtca tctgccgcgc cgcgctcaca ttcgcattgc gcttcttgcc
    10561 ccgaatatcc gccggcatca cattgtaggt gcgggcaacc tcgttgagga tcttttcaat
    10621 cgtcaccggg atcggctgac tgtcattcag cgtgtccttg atggccgtcg tcgtcacacc
    10681 gatgcagggc tcaatgccct ccagcatata gtaggcgttc agtttcttga ccgcgccctc
    10741 cagctggcgg atgttctgct tcaggtgatt tgcaatgtac tcggccacat cgttgggcag
    10801 gtcaagattg agcagctcgg ctttgcgttt gacgatggcg acacgggtct caaaatccgg
    10861 cggctgaatg tcggcggtca ggccccactc aaaccgggtg cgcagacgct cctccagcga
    10921 tttgatctcc ttcgccgggc ggtccgacgc gatgacgatt tgccggccat cgttgtgcag
    10981 cgtgttgaag gtgtggaaaa actcctcctg cgtgctctcc ttgccggcca ggaactggat
    11041 gtcgtcgatg agcagcacat ccgctttgcg gtatttctga cggaactgct ccatcgtcgc
    11101 cgtcttgatg gcggaaatcg tctcgttggt gaacatttca caatccacgt aaacgatgtt
    11161 gaaatccggg tgatttttct tgatctcaaa ctggatggcg ttcaaaagat gggtcttgcc
    11221 caggcctgac ggcccataga tgaacagcgg gttgtatgcg ccggaagggt tcgccgccac
    11281 ggcctgcgcg gccgcaaacg caaactggtt cgacggtccc ttaatgaaat tctcaaaggt
    11341 gaaatcatac cggccgctgg gcagcgattt ctcgtcccgc tggtccgcac tgccatcggc
    11401 cttctcgccg tcctccggcg gggccgccac atagagaatt tccacatcaa agccgagaat
    11461 ggccttgaac gcttctttca gcaacgccgt gtagcgatcg gatacgatct tcatcacaaa
    11521 ttcactgcgc agttccagcg tgatggtgtt ggaattttca aagctcacgg ccttgatatc
    11581 cttgatgtaa aatgcgtagg tagcgtccga ggtcttttcc ttgcagtagg ccttgaccgc
    11641 ttccaatacc tcattgatgg aatccatgaa tgtcgatact ccccacttcc tgtttatgat
    11701 ggaacgccct gtgcggaaaa cagggcgcaa aaatactgaa tacagtatac cacaaaatcc
    11761 ggttttaaac aacagctata tataataaag tagaaacttt tttgtaaggc ggaattgttg
    11821 aaaattttaa gcgcggagga cggtaaaatt ttttgctgga aagttttcca caagaactgc
    11881 atttttcaac agtaaatagt acaaaatgta gtggataact ctttgtagca gcaattcttt
    11941 gacgcgggag cgaaaaattc tgcccaaaac ctcttgacaa ataggtggtt tatcgcgtat
    12001 aatctaactc tgcaaggctg ccccagtgta tgggcgaagc cctgagcaaa cagcccatac
    12061 cgggatttca acaggaggaa tgaaaaatga agcgtacttt ccagcccaag aagcgtcagc
    12121 gcagccgcgt ccacggtttc ctgaaccgca tggctaccaa gaatggccgc aaggtcattg
    12181 cccgccgccg tgcgaagggc cgtaagagcc tgactgtctg attctatcag agatgaaatg
    12241 aacaaaaggg ttgctgattg taaaatcagc aacccttttt tcaaataatg gctttacaga
    12301 aaacgggatt cattataata tagaagaata ggaaaaaata aaatgccttc ccccttgggg
    12361 gaaggtgtcc gcggccccga ccgcggacgg atgagggaaa agtgccccgc agctgttcgg
    12421 tacagggccg gcagggacag gcacgcccct catcagccgc ctgcaggcgg ctgcttcccc
    12481 cctgaggggg aagcctttag gaaggagacc tatgcgttac cgcaccttaa ataaaaattg
    12541 gcagttcaac cgggtgtatg gccggggcaa aagctatgtg catccgcact gcgttttgta
    12601 cgtggctaaa aaccgggtgg gctatacccg catcgggctg accgcaacca aaaaggtcgg
    12661 ccatgccgtg cagcgcaacc gcgcccgccg cgttatgcgc gccgccctgg ccgaacactt
    12721 gtcgcaaaat atcggcggat atgatataat actcgtagcc cgcggccaga ccccgcgctt
    12781 aaaaagcacc cagctgagca aaacgctggg caagctcttc accaaggcgg gtctgcccga
    12841 taaggctgcc cccgccgtgc cggctgccga atgatcacac gctttctcat caaacttttg
    12901 cgcggctaca agcggtatat cagcccacac ctcgggcacc actgccgctt tgtgcccacc
    12961 tgcagcgagt acgccatgca ggcgctggcc acccacggcc tggtcaaagg tctgcttttg
    13021 accacctggc ggcttttgcg ctgccagcca tttgcgaaat tcggctatga cccggtaccc
    13081 ccgaagggga gatgggtcag cccggaacgc aggctgacgc gcagccgctg aaaggcttct
    13141 cctttggggg agaagctgtc cgcaaagcgg actgatgagg ggagaacttt ccattgccgc
    13201 cccgcaaagg ggtggccggg caagcccgcc cctcatccgg ccttcggcca ccttctccca
    13261 agggagaagg cttctcaaca ttgcttctca tgtctttgca ctgcgcagca gagacctaca
    13321 taaaaataat ggtcccgggc gcagacgggg cagaaggaaa ggctatgaac tttatgtact
    13381 tcctggccat attgattggc ccgctggtga agatcctgta cagcttcatc ggcaactacg
    13441 cggtaacgat ggtcgttgcc accctactcc taaaactgct gctcttcccc ctgagcatcc
    13501 atcagcagaa atcctcggca aagatggctg tttttcagcc gctgatgaat gagatccagc
    13561 agaagtacaa aaatgacccc cagaaacagc aggaggagct gatgaagctc cagcaggagc
    13621 acggctttaa ccccatgggc ggctgcctgc ccatgctgct gacgatgctg gtgctgttcg
    13681 gcttcctggg cgttgtgtac taccccgtac actacatctt cggtgtcgat aacaccgccg
    13741 tcaaggctgc gtgcgaggcc atcggcctgg caaccaccaa tgcgtccacg atgcagaccg
    13801 cgctgatcca ggccatccac aacggcgctg tgatcgaccc gtccatcatc cccgccagcg
    13861 tggtttctga gatccaaaac tttaacacca gcttcttcgg catggatatg tgtgatgtcc
    13921 ccggcttccg cctggtgccc atcgccatct tcccggccat cgcagcggtc acgatgttca
    13981 tcagctactt cgtcacccag aagctctccg gtatggatgc ccagatgcag ggcagcatga
    14041 aggtcatgat gctggtcatg aacctgatgt tcgtcacctt ctgctttaac gcccccgttg
    14101 gcttctcgct gtactacggc gtgtccaacc tgctgcagat cggccagtcc tatatcacct
    14161 acaagattta cagccccgag aagttcaagg cccagtatga ggccgagctg gccgccaagc
    14221 gtgccgagcg taagaagaag cgcaccgtca cggtcgaaca gaacggcaag caggtcgaga
    14281 aggaagttac cctgggcgag gccaacaagc tgcgcctgga acgtgcccgc cagctggaag
    14341 ccgagctcta caaagacgag cgcaccaccc cgctgaataa agactaagaa aagacgccgc
    14401 gcaatcgtag ggacgagcat tgctcgcccg cgccgttggc ggctgtgagg gagtgtatcc
    14461 cctgccgccg taggggcgca ttctatatgc gcccgccccg ctcccctttg tgatgaacat
    14521 aacgataaga aataagaagg aggttccctt ttatgcgcag tatggaaatg agcgccaaga
    14581 ccgttgaagc cgccgtgcag gccgcctgcg aagccctggg cgttgaccgt gacgatatca
    14641 atgtcagcta tgaagtgctg gaatttccca cccgcaagct gttcaaaacc atccctgcca
    14701 aggttctggt aaaggtcgag gaacccgagg ccgccccggc cccggctgct gaggaaaagc
    14761 ctgccgaagt ttccaccaag ccggcagaag ttgttgaaat ctccgacgag accgcccccg
    14821 aaatcgagaa agcagtcgtt gaggatatgg tcgaggccgc cgagctggcc ccggttgccg
    14881 aggacgacgt tgagatccct ctcgatatcg ccgctgaccc gcgcctgcag gctgcggttg
    14941 actacctgac tcccatcttc aacctgatgg gtgtggaaaa cttcagcttc accgccgtta
    15001 agaagggcgc tgccaccatc ctgaaagtct ccggcgagca catgggcgca ctcatcggcc
    15061 gccgcggtga gacgatggag agcctgtcct acctcgccag cctcgttgtg aaccgcatgg
    15121 aaggccccta tatcaagctg ggcctggatg tcggcggcta ccgcaacaag cgcgaggacg
    15181 acctctctgc ccttgccaag cgcattgcag accgtgtcat ccgcaccggc tgctactacg
    15241 agatggagcc gatgaacccc tacgagcgcc acatcatcca caccgccatc gccgatatcg
    15301 agggcgtgcg cagcgagagc aagggcgaag gcccggcccg ccatgtggtg ctttactcca
    15361 ccgaccccaa cgccagcaac ctgcccgatc gcgataacgc ccgcaaccag cgcggtggcc
    15421 gccgtgacgg cggtcgtgac ccccgccgtg atggaaaccg cggctaccgc ggcaacggcc
    15481 ccagaggccc ccgcagcggc cgtgacggcc gtgatggcgg gcgcggcggc taccgcggca
    15541 atcgcggcga ccgtggcccg cgctacggcg gcccgcgttc cagtgtgccc gcccgcgact
    15601 ttgccgaccg cccgcgtgac ccggatgcca agccgatggc ccccaaaacc accgagcgca
    15661 tccacgacgg tgacgatttc gcgtttggta agatcgagtt ttaaacgaaa ttgttgaaaa
    15721 agcagcctga ctgtaaagcc gggctgcttt ttttggtggg tggcaaggcg gaggggcttt
    15781 gcccgcgcct gattgtggac aactgcccta ctttccacac cgttttccac aaccgtgttg
    15841 aaaactctta tttttccacc acccacaacc gggtatacat tagggtcaaa atccccaatt
    15901 attaacaata tatagacata cccggttgct tat
//
LOCUS       DBMDSQ010000016        15846 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_1175_length_15846_cov_1.678882, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000016 DBMDSQ010000000
VERSION     DBMDSQ010000016.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 15846)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 15846)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15846
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1175_length_15846_cov_1.678882"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            4..276
                     /locus_tag="ACZPAX_01235"
     CDS             4..276
                     /locus_tag="ACZPAX_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030527 - structural constituent of
                     chromatin [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HIR1112552.1"
                     /translation="MTKVELIAAVANEANLTKKDAEAAVNSTLNAITNALKEGDKVTL
                     VGFGTFEVRERPARKGRNPQTGEEITIEASKLPAFKAGKALKDSVQ"
     gene            356..601
                     /locus_tag="ACZPAX_01240"
     CDS             356..601
                     /locus_tag="ACZPAX_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015558083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HIR1112553.1"
                     /translation="MRLDKYLKVSRVIKRRTLANEVADAGRVLVNGKPAKASYTVKVG
                     DIIEVTFGNRPVKIRVLSDVEQKGKDVAREMFEVIEG"
     gene            687..1001
                     /locus_tag="ACZPAX_01245"
     CDS             687..1001
                     /locus_tag="ACZPAX_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YabP/YqfC family sporulation protein"
                     /protein_id="HIR1112554.1"
                     /translation="MPETKLEPRTPRAEPAPQQPKSHGLALKDRRHLAVTGVSRVISC
                     DENTAVLETPLGNLTIGGQELQIGELSTQSGQVQLSGKIEYLQYAENQQSGGGLLARL
                     FR"
     gene            1001..1480
                     /locus_tag="ACZPAX_01250"
     CDS             1001..1480
                     /locus_tag="ACZPAX_01250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1112555.1"
                     /translation="MAPAPAPWAVLGDALWCLGLGCLLAAARDALRLALGEGKLRCFL
                     RDAAFFAAAAVLICGFAAGTSAGRLARWYMSAAVLLGALCWRWAVQPAVHRAASMLAV
                     LLLGPLHAAHKMLLQPLQDRAAQARTARKSRKTAKKSRKTQNSAEKQLQKPSIILYN"
     gene            1529..1828
                     /locus_tag="ACZPAX_01255"
     CDS             1529..1828
                     /locus_tag="ACZPAX_01255"
                     /inference="COORDINATES: protein motif:HMM:NF016839.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsB family cell division protein"
                     /protein_id="HIR1112556.1"
                     /translation="MNKRKGLSSQGFLPWWITVPVFLIICGSLFVNLVQYQVSIASKQ
                     QELQSVQNQLSAQLTENAELSGTLEQGDSAIIERYAREQGYAKPNERVFVDISGK"
     gene            1901..2383
                     /locus_tag="ACZPAX_01260"
     CDS             1901..2383
                     /locus_tag="ACZPAX_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HIR1112557.1"
                     /translation="MALQVGDIVEGKVTGIKPFGAFVSLPEGKTGLVHISEVSYEFVQ
                     DLSTVLSDGQTVSVKVISIAPDGKIALSIKRTQPAPERGQRPPRGEHNGNGGDRRSAP
                     RPKREEKPRVWQPKPAAPQGEMSFEDMMARYKTRSEEKIADLKRVTENHRGGYSRRRG
                     "
     gene            2480..3619
                     /locus_tag="ACZPAX_01265"
     CDS             2480..3619
                     /locus_tag="ACZPAX_01265"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing class I SAM-dependent
                     RNA methyltransferase"
                     /protein_id="HIR1112558.1"
                     /translation="MSTLYTMVAPCFFGTESTLNFEVKRLGAQNIQVTDGRVAFQGGA
                     DVIAAANLNLRTAERVLLLLATYKATTFDELFDGCYNIAWEELLPANAAFPVKGSSLS
                     SQLSSVPACQIIVKKAIVKRLMQGHKVSTLPEDGALYKVRFALRKDTVEIYLDTSGDG
                     LHKRGYRKNATLAPIKETLAAAIADLGRVRRDSLVQDPFCGSGTLVSEAAQKALNLAP
                     GLRRRFAAEHFDFVPADIWAEQRQKALSEVRKDAAFEGIGYDIDPAAVALANANAKLA
                     GVGDRCRFETADVKDFRALDSATVLTNPPYGERLGDASEAAGLAKTLGQVWQAHPAQG
                     LYAITADADFEQHFGKKAARRRKIYNGMIPCQLYMYFEQPKRERK"
     gene            3623..4447
                     /locus_tag="ACZPAX_01270"
     CDS             3623..4447
                     /locus_tag="ACZPAX_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HIR1112559.1"
                     /translation="MEDLKLAILIDADNISPKYVKVILDEAATFGVAACKRIYGDWSD
                     VRLKSWKDALLNNSIIPIQQYSYTTGKNATDSAMIIDAMDLLYSGNLDGFCIVSSDSD
                     FTRLAARLREAGKLVIGMGESKALGPFVKACDQFKYLDLILDHGGDEDAAPAAPAAET
                     SARANAGDDAAINRDSIGRIIHGLIDEMDDGTGWVRTSRVGDQLIKRYPDFDVRNFGS
                     KSLNKFLASLPELEVEERILANGNPIQFVHVKPTPKPAKKPPQRRTVKRTTRKKKQ"
     gene            complement(4491..5849)
                     /locus_tag="ACZPAX_01275"
     CDS             complement(4491..5849)
                     /locus_tag="ACZPAX_01275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HIR1112560.1"
                     /translation="MKKVLSPLLSALLLAGCAAGTDVPTVQPTAAPETPDTAQSSGTA
                     ETAGAAAAPQGLIPTDAPVLDYDDIRLIAYVREFQTYPDTDYYTMCRDGKWGLMRSDG
                     TEVLPCRAPYPLFECGWNAHHWHGYTDGMTWEEIEPLQEQLNAQLKETGDGVLCDAHD
                     GGGYRMFVYLQDASVYVYAGSIGPGEFAAPTDEALCLYSGRADGIVPTVSGTTTHESD
                     DWYNFVSDGVYVYRDKNGTAANSCTYSAADFFFDAPLAPAQRDGRWVYLDTTGREATG
                     LCYDAVYDFDIYSDTPLTAARAAPLLSGYAVVCRDGQFGLLDGSGAEFVPCTYDGLVW
                     DGSTAWVKQNDGWHEYTIPGVAKPDPLDTLSGDIVAPDTRPTRTDTVYFRADADSSNR
                     LNLRTGPGTEYDIIDRISSSTLRVYGYASAAPGWALVRYDPLYGMPHFGWVSTSYLLP
                     AE"
     gene            complement(5955..6848)
                     /locus_tag="ACZPAX_01280"
     CDS             complement(5955..6848)
                     /locus_tag="ACZPAX_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009324390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HIR1112561.1"
                     /translation="MPILNSYYQEVYRSPVVRLDGYSGRHGLEAGILAYLDFGGATGT
                     SKDGLAETMLAFATERGTLQPGMPVVEASSGSFGAALAVSCATTGHPCILVVPSNLPI
                     ARRKHLQDLGARIIACSSGGRRAMDRIAADTAKRYGGYYTHYFSNDDNPEYHRRVTGP
                     QILKHAGDAIDAIVIGVGSGGTITGVAEYIKAWNSMVRIVAVEPAECAAISGGFIGQH
                     GIAGIGPGFVPENYNPYVVDTVLTVTTADAERAAREVLFFDGVPACTSAGATLAAAVQ
                     LMGMGKAKRPLCVFAGRRTYE"
     gene            7238..7636
                     /locus_tag="ACZPAX_01285"
     CDS             7238..7636
                     /locus_tag="ACZPAX_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015536880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HIR1112562.1"
                     /translation="MAGKPQKNLAYSVLLDFYGPVLTDKQRVILTEYYDEDLSLAEIA
                     ENLGITRQGVRDAIKHGEAALDELEQKLGNARRHTQMRADLDRLRVLVTEIRCCNSGS
                     FTPDPRINHDTQEMLTILQRLDTQEDADGL"
     gene            7626..8975
                     /gene="ffh"
                     /locus_tag="ACZPAX_01290"
     CDS             7626..8975
                     /gene="ffh"
                     /locus_tag="ACZPAX_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HIR1112563.1"
                     /translation="MAFESLTERLNGVFKKLRGKGKLNEADIKAAMREVRMALLEADV
                     NYKVAKDFCAQVSERAMGQEVMESLTPAQQVVKIVNEELTNLMGGNEPKYLRLKNKGQ
                     TVLMMCGLQGNGKTTHAAKLGKYYRSQGRRPLLVACDIYRPAAIEQLKVVGEQAGVPV
                     FTLGTEKPEIIAQKAMSYAKDHGNDVVILDTAGRLQIDDQLMDELVRIKQAVEVDETL
                     LVVDAMAGQEAVNVAKTFNEKVGISGVILTKTDGDTRGGAALSVLAVTGMPIYFQGTG
                     EKLEDLEPFYPARMANRILGMGDVLSLIEKAQSVQNDKEAEAAAKRLMENKFDMNDLL
                     TQFQQVKKMGGASALLAMMPGGNQIDPDSLDEKALPRIEAIIYSMTPAERAKPDIINP
                     KRKRRIAAGSGTSVEDVNKLLRQFEMMQKMMKKVKRNPKGFMRSLGNMGGFKGFGRK"
     gene            9074..9313
                     /gene="rpsP"
                     /locus_tag="ACZPAX_01295"
     CDS             9074..9313
                     /gene="rpsP"
                     /locus_tag="ACZPAX_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HIR1112564.1"
                     /translation="MVKIRLRRMGAKKAPFYRVVVADSRFARDGRFIEEIGYYDPTKE
                     PSVVKIDAEKAKQWLDNGAQPTDTVREILKKAAVL"
     gene            9345..9578
                     /locus_tag="ACZPAX_01300"
     CDS             9345..9578
                     /locus_tag="ACZPAX_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015565586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HIR1112565.1"
                     /translation="MQELLLAVARGLVEDKEAVQVTVDPMREDGTVVYHLKVAEADMG
                     RVIGKQGRIAKAIRVVMRAAAVRQGEKVIVEID"
     gene            9640..10170
                     /gene="rimM"
                     /locus_tag="ACZPAX_01305"
     CDS             9640..10170
                     /gene="rimM"
                     /locus_tag="ACZPAX_01305"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HIR1112566.1"
                     /translation="MQNYLPACKIVSTHGVRGEMKALPLCDGAAFLAKFKRLFTSADG
                     AGETRVLGVRAQGNVILLRLDGVTDMDAARAQVGRTYYLAKADAKLPRGRYFIDDLLG
                     CDVVDADTDRVYGQLTNVDRPAAQDIYTVTDGAGEEHMLPAVPEFVKKIDIDARKIFV
                     TPIEGMFTDAVNGDED"
     gene            10175..11404
                     /gene="trmD"
                     /locus_tag="ACZPAX_01310"
     CDS             10175..11404
                     /gene="trmD"
                     /locus_tag="ACZPAX_01310"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005944688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HIR1112567.1"
                     /translation="MRIDIVTLFPELCDSFLSASILGRARAKNLFEAHCHQIRDYTKN
                     KQKQTDDYPYGGGCGMVLYAQPIADCLRAVQAQCAGQGRQKPHVVFLTAAGRPYNEEK
                     ARELASYDAVTLVCGHYEGIDQRVIDAFGDEEISIGDYVLTGGELASLVVADSVLRLQ
                     PGVLAEEKGYQDESYWDGLLEYPQFTRPEVWEGRAVPPVLLTGDHKKIDEWRGAQSRE
                     RTRERRPDLYDAWCESHPLTELPKWKRGENMRLVKNDEQLALCAALMAEGRRTVCAPV
                     CSEEYLAKMTPEFWLPELADKKRNGWAFYLHYTKDAADGMVGVCHKTGELGHLFVTES
                     ARGKGYGAKMLDFARKKLAEHPHPTFGVLSTNTRAIALYKRMGWRLTGTVLNVYDPEN
                     DAFTEKYCEEWQMRYQG"
     gene            11656..11919
                     /locus_tag="ACZPAX_01315"
     CDS             11656..11919
                     /locus_tag="ACZPAX_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005352659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HIR1112568.1"
                     /translation="MYVKEVTVENQVGLHARPATFFIQKANEYKSSIWVEKEERRVNA
                     KSLLGVLSLGIVGGTTIRIIADGADEQAAVDGLVKLVSSGFAE"
     gene            11983..13833
                     /gene="uvrC"
                     /locus_tag="ACZPAX_01320"
     CDS             11983..13833
                     /gene="uvrC"
                     /locus_tag="ACZPAX_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HIR1112569.1"
                     /translation="MTKAELYKKACMLPLLPGVYIIRDKTDTIIYIGKAKRLKTRVSQ
                     YFREGVPHDAKVSQMIAHAFTFDVIVCQSEFEALVLEASQIKAHTPKYNILLKDDKGY
                     SYVKVTRGAWPRISAALQKDDDDADYIGPFTSSFAVREMVETAQDCFLLPRCNKNFPQ
                     DFRKGRPCLNAHIGKCMAVCSGKITCEAYNDAVQGALRMIRYGKKDIVKQLREKMEAA
                     SERLDFETAALLRDQIMAIDRVAAGQKVVMENDTEMDVIALAGTTHAVCAAVLRYRDG
                     RLTDKREFLFRDTTDINAVREEFLPQYYLDGGAIPKTIAVDALPPDADALSEALNQAR
                     GTKVELYVPQRGDVAKLVTMAYTNAVERLGRESGRYTREEKLLEEAAQVLGLKKPPRV
                     IESYDISNWGDGSSVCGMVVFRDGRPHRAGYRRFKMKTVAGTDDYASLAETLSRRAAE
                     YEMARRGEKPDGPQNQFATLPDLLLIDGGRGQVGAVKQALRGTALEHVPTFGMVKDDH
                     HRTRAIVDDEGGEIAINRHRNIFTFVTNIQDETHRYANDYRKRAMKKKSYAATLTAIP
                     GVGEKTSAALLAHFKTVSAVKAASISDLEEVKGISHAKAEILYNALRNGV"
     gene            13873..>15846
                     /locus_tag="ACZPAX_01325"
     CDS             13873..>15846
                     /locus_tag="ACZPAX_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1112570.1"
                     /translation="MLLLTLLLSLALAAFFTLLPARVLHRRTTRADLYVGAGVAVLVW
                     LWAACVWSKPGLTVDFDGFRLAALLAMQLLACGVVTGFRLRQMLPTKLRTPAAVGLLA
                     AAALGLELFVGNLNWLATHGYTPIDLRPYLTADADPTAPIVLNDENTTLQFIGLDFPI
                     YNLQLDGLVSLADGDTPEQKNVRLNLNVSATDEAATVSRQSWNWPVAAASARSWARSL
                     DLSGKADSLTLTASGFNGEYRSYPLNAQLNTVYANARRPLDFSVLRFAVVLALLLAGF
                     ALRPASVLWRDAYAAHERKYRPAVLAVELALCAAAFLAPFGDRFNAGVATSFYNTPDW
                     SGTSRIDFTMHINDWASNTAAQYGALAHSFLQGRLDLEKDPPAAMADLANPYDTAARQ
                     DAAPDALWDVAYYNGRYYVYFGVVPCLLFQLPFEALTGIRDLPPSLPMIFLAWLYIFA
                     VFGFIRQAVRRWFPNASAAACLLTAAGAASGSQIYYLLHRPSVYEYAILSGAAFVLLA
                     LWQWLCAANAPETKRKTILFHLAFGSLCMALVAGCRPQMVLFAVLALPILRPRYIIQK
                     RLRSRAGAGECAAFLLPVVLVAVSLMWYNAARFGSPFDFGANYNLTSNDMTRRGFAVG
                     RIAPAVVTFLAGIPGVQTVFPYITATKMQTNYMG"
BASE COUNT         3311 a         4907 c         4734 g         2894 t
ORIGIN      
        1 accatgacga aagttgaact gatcgccgct gttgcgaacg aagctaacct gaccaagaag
       61 gacgccgagg ctgctgtcaa cagcaccctg aacgccatca ccaacgccct gaaggagggc
      121 gacaaggtca cgctggtcgg cttcggtacc tttgaggtcc gcgagcgccc cgcacgcaag
      181 ggccgcaacc cccagaccgg cgaggaaatc accattgagg cttccaagct gcccgctttc
      241 aaggccggca aggccctgaa ggactccgtc cagtaatcgt aaattgactg gcccgcccca
      301 ccgcgtcttt gcggcggggc ggttttgtat caaaccgaaa ggaaacatac ctaccatgcg
      361 tctggataaa tacctgaaag taagccgcgt catcaagcgc cgcaccctcg ccaacgaggt
      421 cgccgacgcg ggccgcgtgc tcgtcaacgg caagccggcc aaggccagct ataccgtcaa
      481 ggtcggtgat atcattgaag tgacctttgg caaccgcccg gtgaaaatcc gcgttctctc
      541 cgacgtggag cagaagggca aggacgtcgc ccgggaaatg tttgaggtca tcgaagggtg
      601 agcgggcttt gccgccttgc cgttggttct actttcccct gtacctgcat atagtaccac
      661 tgtaaccaaa aaggggagga accatcatgc cggaaacaaa actggaacct cgcacacccc
      721 gtgcggagcc tgcgccgcag cagccaaaaa gccacggcct ggcgctgaaa gaccgccgcc
      781 acctggctgt gaccggcgtc agccgggtca tcagctgcga cgaaaacacc gccgtgctgg
      841 aaacgccgct gggtaacctg accatcggcg ggcaggagct gcaaattggc gaattgtcca
      901 cccagagcgg ccaggtgcag ctttccggca aaatcgaata cctgcaatac gccgagaacc
      961 agcaatcggg cggcggcctg ctggcccggc tgttccgctg atggcccccg cgcctgcccc
     1021 ctgggccgtg ctgggggatg ccctgtggtg cctggggctg ggctgcctgc tggccgcagc
     1081 gcgggatgct ctgcgcctgg cgctggggga aggaaaactc cgctgctttt tgcgggatgc
     1141 cgcctttttc gccgcagcgg cggtgctcat ctgtggcttt gcggccggga ccagcgcagg
     1201 cagattggcc cgctggtaca tgagcgccgc agtgctgctg ggggcgctgt gctggcgctg
     1261 ggccgtgcag cccgccgtac accgggcggc gtcaatgctg gcggtgctgc tgctcggccc
     1321 gctgcacgca gcccacaaaa tgctgctgca gccgctgcag gatcgcgccg cacaggcccg
     1381 cacggcgcgc aaaagccgca aaactgcaaa aaaatcccgg aaaacccaaa attccgccga
     1441 aaaacagttg caaaagccgt cgataatatt atataattaa ctatgcgtaa atttgtgtgc
     1501 atttttatgg gaaagggggc accctacaat gaacaaacgc aaagggctgt cctcgcaggg
     1561 attcctgccg tggtggatca ccgtgccggt gtttctgatt atctgcggtt cgctgttcgt
     1621 caatctcgtg cagtatcagg tgtctattgc gtccaaacag caggagctgc aatccgtgca
     1681 gaaccaactc agcgcacaac ttaccgaaaa cgctgaactt tccggcacgc tggagcaggg
     1741 cgacagcgcc atcattgagc gctatgcccg cgagcagggc tacgccaagc ccaacgagcg
     1801 cgtgtttgtg gatatcagcg gcaaataaat cgtgttacca gcgggggcgc tttgcggcaa
     1861 ccccctgtaa taaaaatcat ttttaaaggg gtatcatagt ttggcattac aagttgggga
     1921 tattgtcgag ggcaaggtca ctggtatcaa gccgttcggc gcgttcgttt ctctgccgga
     1981 gggcaagact ggtcttgtcc acatctccga ggtttcctac gagttcgtac aggatctttc
     2041 cacggtgctc agcgatgggc agactgtttc cgtcaaggtg atttcgattg cacctgacgg
     2101 caaaattgca ctgtccatca aacgcaccca gcccgcaccg gagcgcggcc agcgcccgcc
     2161 gcgcggtgag cataacggca acggcggcga ccgccgcagt gcgccgcgcc ccaagcgcga
     2221 ggaaaagccc cgcgtatggc agcccaaacc cgctgcccca cagggcgaaa tgagctttga
     2281 ggacatgatg gcccgctaca agacccgcag cgaggaaaag atcgcggacc tcaagcgcgt
     2341 gactgaaaat caccgcgggg gctactcccg ccgccgcggc tgattttggc tgcccagcta
     2401 ccagaacgaa cggccctatc ctgacggctg tcagccgccg ggctggggtc tttttgcaat
     2461 tttacaagaa aggacaccta tgtctacact ctataccatg gtcgcaccgt gcttcttcgg
     2521 cacggagtcg acgctgaatt ttgaggtcaa gcgcctgggc gcacagaaca tccaggtcac
     2581 ggatggccgc gtggcgttcc agggcggggc agacgtgatc gccgccgcca acctgaactt
     2641 gcgcacggcc gagcgcgtcc tgctgctgct ggctacctac aaagccacga cctttgatga
     2701 actgttcgac ggctgctaca atatcgcgtg ggaggaactg ctccctgcca acgccgcctt
     2761 cccggtcaag gggtccagcc tgtccagcca gctttccagc gtgcccgcct gccagatcat
     2821 cgtcaaaaag gccatcgtca agcggctgat gcagggccat aaggtcagca ccctgcccga
     2881 ggacggcgcc ctgtacaagg ttcgattcgc cctgcgcaag gacaccgtcg agatttacct
     2941 cgacacctcc ggcgacggcc tgcacaagcg cggctaccgt aaaaacgcga cgctggcccc
     3001 catcaaggag acgctggccg ccgccattgc cgacctgggc cgtgtgcgcc gtgactctct
     3061 cgtgcaggat cccttctgcg gttccggcac gctggtcagc gaggctgcgc agaaagcgct
     3121 gaatctggcc cccggcctgc gccgccgctt tgccgccgaa catttcgact ttgtccccgc
     3181 tgatatctgg gccgaacagc gacagaaagc cctctccgag gtccgcaagg atgctgcttt
     3241 tgagggcatc ggctacgata tcgaccccgc tgccgtggcc ctggccaacg ccaacgcaaa
     3301 gctggcgggc gtgggggacc gctgccgctt tgaaaccgcc gatgtcaagg acttccgcgc
     3361 cctcgacagt gccaccgtcc tgacgaaccc gccctacggc gagcgcctgg gcgacgccag
     3421 tgaggccgca ggccttgcca aaacgctggg ccaggtctgg caggcccacc cggcacaggg
     3481 gctgtacgcc atcacggcgg acgcagattt tgaacagcac ttcggcaaaa aggccgcccg
     3541 tcgccgcaag atctacaacg gcatgatccc gtgtcagctc tatatgtact ttgagcagcc
     3601 caagcgcgag aggaagtaag ccatggaaga cctgaaactg gcaatcctca tcgacgcgga
     3661 caatatttcg cccaagtacg tcaaagtgat tctcgatgag gccgcgacct tcggcgtcgc
     3721 cgcttgcaag cgcatctacg gcgactggtc cgacgttcgg ctgaaaagct ggaaggacgc
     3781 gctgctgaac aattctatca tccccatcca gcagtacagc tacaccaccg gcaaaaatgc
     3841 gacggattcc gctatgatca ttgacgcgat ggacttgctg tacagcggca atctcgacgg
     3901 cttctgcatc gtctcgtcgg acagtgactt tacccgcctg gccgcccgcc tgcgcgaggc
     3961 cggcaagctg gtcatcggca tgggcgagag caaggctctc ggccccttcg tcaaggcctg
     4021 cgaccagttc aaatatctgg atctgattct cgaccacggc ggggacgagg acgccgcccc
     4081 ggcagccccc gccgccgaga caagcgcccg cgccaacgcg ggggacgatg ccgccatcaa
     4141 ccgagattcc atcggccgca tcatccacgg cctgattgat gaaatggacg acggcaccgg
     4201 ctgggtgcgc acctcccgcg tgggcgacca gctcatcaag cgctacccgg acttcgacgt
     4261 gcgcaacttc ggctccaaga gcctgaacaa gttcttagcg tccctgccgg aactggaagt
     4321 cgaggagcgc atcctcgcca acggaaatcc catccagttt gtccacgtca agcccacccc
     4381 gaaacccgcc aagaaaccgc cccagcgccg caccgtaaag cgcacaacgc ggaagaaaaa
     4441 acagtaaaag aagcagcccc ctgaacgcat cacgctcagg gggcattttt ctattccgca
     4501 ggcagcaaat acgacgtgct cacccagccg aaatggggca tcccatacag cggatcatac
     4561 cgcaccagtg cccagccggg ggcagcgctg gcgtacccat acacccgcag cgtgctggaa
     4621 gatatccggt cgatgatatc atattccgtc cctggcccgg tgcgcagatt caaacggttg
     4681 ctgctatcgg cgtccgcccg aaaatacaca gtatccgtcc gagtgggccg cgtatccggg
     4741 gcgacgatat ccccggacag cgtatccagc gggtcgggct ttgccacacc gggaattgtg
     4801 tactcgtgcc agccgtcgtt ctgcttgacc caggctgtgc tgccgtccca cacaagaccg
     4861 tcataggtgc aggggacaaa ctcagccccg ctgccatcca acaggccaaa ctgaccatcc
     4921 cggcacacga ccgcatagcc gctcagcagc ggtgcggcgc gggcggcggt cagcggtgtg
     4981 tcggagtaaa tgtcaaaatc gtacacggca tcatagcata agcctgtcgc ctcgcggccc
     5041 gttgtgtcga gatataccca gcggccatcc cgttgggcag gggccagcgg tgcgtcaaag
     5101 aagaaatcag ccgcgctgta tgtgcagcta ttagccgctg tgccgttctt gtcccgatac
     5161 acatagaccc cgtcgctgac gaaattgtac caatcgtcgc tctcgtgggt ggtcgtgccg
     5221 ctgaccgtgg ggacgatgcc gtcggcgcgg ccactgtaca ggcatagggc ctcgtcggtg
     5281 ggggcggcga actcgccggg accgatgctg cctgcgtaga cgtacacgct ggcatcctgc
     5341 aaatagacaa acatccgata gccgccgccg tcgtgggcgt cgcagagcac accgtcgccg
     5401 gtctccttca gctgggcgtt caactgctct tgcagcggct cgatttcctc ccaggtcatg
     5461 ccgtctgtgt aaccatgcca gtgatgggcg ttccagccgc attcaaacag cgggtacggt
     5521 gcgcggcagg gaagaacctc ggtgccatcg ctgcgcatca ggccccattt gccgtccctg
     5581 cacatggtat agtagtcggt atcaggatag gtctggaact cccgcacata ggcaatgagc
     5641 cggatgtcgt cgtagtccag cacgggcgca tcggtcggga taagaccctg gggcgctgcc
     5701 gcggccccgg cggtttccgc agttcccgag gactgtgccg tgtcgggcgt ttcgggcgcg
     5761 gcggtgggct gcacggttgg cacatctgtc ccggcggcac agcctgccag cagcaaagcg
     5821 gataacaggg gagacaaaac ttttttcacg gcattcaccg cctttttttt cttttttctt
     5881 tagtatagca caagcgcccc gcaccgggaa atacagtgca gggcgcttgt gcaaatttta
     5941 caaatattac aaaattattc gtaggtacga cgcccggcaa acacgcacaa ggggcgcttg
     6001 gccttgccca tgcccatcag ctgcacggcg gcggccaggg tggccccggc gctggtgcag
     6061 gcggggacac cgtcaaagaa cagcacctcc cgcgcggcgc gctcggcgtc ggcagtggtc
     6121 acagtcagca cggtatccac gacgtaaggg ttgtagtttt ccggcacaaa gcccgggccg
     6181 atgcccgcaa tgccgtgctg cccgatgaac ccgccggaga tggcggcaca ctcggcaggc
     6241 tccaccgcga cgatgcgcac catgctgttc catgccttga tgtactcggc cacgccggtg
     6301 atagtgccgc cgctgcctac gccgatgacg atggcgtcaa tggcgtcgcc tgcgtgcttc
     6361 aaaatctgcg gcccggtcac gcggcggtga tattcggggt tgtcgtcgtt ggaaaaatag
     6421 tgggtgtagt acccgccgta ccgcttggca gtgtcggcgg caatgcggtc catggcccgg
     6481 cggccgccgc tgctgcacgc aatgatacgc gcacccaagt cttgtaaatg cttgcgccgc
     6541 gcaatgggca ggttgctggg cacgacgagg atgcaggggt ggccggtcgt ggcacagctg
     6601 accgccagcg ccgcgccgaa gctgccggag ctggcttcca ctaccggcat accgggctgc
     6661 aaagtgccgc gctcggtggc aaaggcgagc atcgtctcgg ccaggccgtc cttcgaggtg
     6721 cccgttgccc cgccgaaatc gagataggct aggatgcccg cttccagccc atggcggccg
     6781 gaatagccgt ccagccgcac cacgggggaa cggtagactt cctgataata gctgttcagg
     6841 atgggcatag ggggcacctc actttcaaaa aacgacaaca tttctattat agcatagttc
     6901 agggtggttt gctatggatg gagagaaaaa tattatgacc aaagggaaaa gcccctcagg
     6961 cctcgcaggc tcggctagct ccccacttcg tggggcatct tttgcgcggg caaagcccgc
     7021 gagaaagcct cctcgctttg cggggaggtg acgagcgcag caaagtcgga ggggcattct
     7081 ccgtttgcca tcacggcgct gctaaaaaat ctgccgtatt tcttcaaaaa atcgctccac
     7141 agacttgact tccggcatcg gttctgttat aattcatagg ctgatgtaaa gaaaattact
     7201 ttacacagct ttcacaagca gcaaaggggg cgcgggcatg gccggcaagc cgcagaaaaa
     7261 ccttgcctac tccgtcctgc tggacttcta cggcccggtc ttgacggaca aacagcgcgt
     7321 catcctgacg gaatactatg acgaggatct gtccttagcc gaaatcgccg agaacctcgg
     7381 catcacgcgg cagggcgtgc gggacgccat caagcatggc gaggcagcgc tggacgagct
     7441 ggagcaaaag cttggcaatg cgcgccgcca cacccagatg cgggccgatt tggatcgtct
     7501 gcgggtgctc gtgacagaaa tccgctgctg caactccggc tccttcacgc cggatccgcg
     7561 catcaaccac gatacgcagg aaatgcttac gatcttacaa cgcttggaca cgcaggagga
     7621 tgccgatggc ctttgagtct ttaacagagc gcttgaatgg cgttttcaaa aaactgcgcg
     7681 gcaagggtaa gctgaacgag gccgatatca aggccgccat gcgggaagtc cggatggcgc
     7741 tgctggaagc cgacgtcaac tacaaggtgg cgaaagactt ctgcgcccag gtctccgagc
     7801 gtgccatggg ccaggaggtc atggagagcc tgacccccgc ccagcaggtc gtcaagatcg
     7861 tcaacgagga actgaccaac ctcatgggcg gcaatgagcc gaagtatctg cgcctgaaaa
     7921 acaagggcca gaccgtgctg atgatgtgcg gcctgcaggg caacggcaag accacccacg
     7981 ccgcaaagct cggcaaatat tatcgcagcc agggccgccg cccgctgttg gtcgcctgcg
     8041 atatctaccg ccccgctgcc attgagcagt tgaaggtggt gggcgagcag gccggtgtgc
     8101 cggtgttcac cctcggcacc gaaaagcccg agatcatcgc gcagaaggca atgtcttatg
     8161 cgaaggatca cggcaatgat gttgtcattc tcgataccgc aggccgtctg cagatcgatg
     8221 accagctcat ggatgagctg gtgcgcatta agcaggcggt cgaggtcgat gaaactttgc
     8281 tggtcgtgga cgccatggcc ggtcaggaag ccgtcaacgt ggcaaagacc ttcaatgaga
     8341 aggtcggcat cagtggtgtg attctgacca agaccgacgg cgacacccgc ggcggcgcgg
     8401 cgctttcggt gttggccgtg accggtatgc ccatctattt ccagggtacc ggcgaaaagc
     8461 tcgaggatct tgagccgttc tacccggcac gcatggcaaa ccgcatcctt ggcatgggag
     8521 atgtgctttc cctgattgag aaagcccaga gtgtccagaa cgacaaggaa gccgaggccg
     8581 ctgccaagcg cctgatggag aacaagttcg acatgaacga cctgctgacc cagttccagc
     8641 aggtgaaaaa gatgggcggt gcgtcggcgc tgttggccat gatgcccggc ggcaatcaga
     8701 tcgatcccga ctccctcgat gaaaaggcac tgccccgcat cgaggccatc atctactcaa
     8761 tgaccccggc ggaacgcgct aaacccgaca tcatcaaccc caagcgcaag cgccgcattg
     8821 cagcaggttc cggcacctcg gtggaggacg tgaacaagct gctgcgtcag tttgaaatga
     8881 tgcagaagat gatgaaaaag gtcaagcgca accccaaggg cttcatgcgc agcctgggta
     8941 acatgggcgg cttcaaaggc tttggccgca aataatttta ggtatgaccc gctcggcttt
     9001 acggcccacg ggatgtacaa tatattaaag atgccccgca agggccgtaa acaatttttg
     9061 gaggaatatt actatggtta agattcgtct gcgccgcatg ggcgccaaga aggctccttt
     9121 ctaccgcgtt gttgttgctg acagccgctt cgcccgtgat ggccgtttca tcgaggaaat
     9181 cggctactac gatccgacca aggagccttc tgtcgtcaag atcgacgctg agaaggccaa
     9241 gcagtggctg gacaacggtg ctcagcccac tgacaccgtc cgcgagatcc tgaagaaggc
     9301 cgccgttctg taatttgttc gctttttagg gagggaacgc cacaatgcag gaactcttat
     9361 tagccgttgc ccgcggcctt gtcgaggaca aggaggccgt gcaggtgact gttgatccca
     9421 tgcgcgagga tggcactgtt gtctaccacc tcaaggtggc cgaggccgat atgggccgcg
     9481 tcatcggcaa gcagggccgc atcgccaagg cgattcgcgt tgtcatgcgt gccgctgccg
     9541 tccgtcaggg cgaaaaagtc atcgtcgaga tcgactaaga cccgaaccaa agcactttgc
     9601 cgcagggtaa agtgcttttt ctgtatcagg aggaacccta tgcaaaacta tcttcccgcc
     9661 tgtaaaatcg tctccaccca tggcgtgcgc ggcgaaatga aagccctgcc gctgtgcgac
     9721 ggcgcggcgt tcctggcaaa gttcaagcgc ctgttcacca gcgctgacgg cgcaggggag
     9781 acccgcgtgc tgggcgtgcg tgcccagggc aacgtcatcc tgctgcggct ggacggcgtc
     9841 accgatatgg acgccgcccg cgcccaggtg ggccgcacct actaccttgc caaggccgat
     9901 gccaaactgc cccggggccg ctatttcatt gatgacctgc tgggctgtga cgttgtggat
     9961 gccgacactg accgcgtcta cggccagctg acgaatgtgg accgccccgc cgcccaggat
    10021 atctatactg tgaccgatgg ggcaggcgag gagcatatgc tgcccgcagt gccggagttc
    10081 gtcaaaaaaa tagacattga cgcgcgtaaa attttcgtga cgcccatcga gggtatgttc
    10141 accgatgccg tcaacggcga tgaggactga gccgatgcgc attgatattg taacgctgtt
    10201 cccggaactg tgcgacagct tcctttcggc cagcatcctg ggccgcgccc gggccaagaa
    10261 tctgttcgag gcccactgcc accagatccg cgattacacg aaaaacaaac agaagcagac
    10321 cgacgactac ccctacggcg gcggttgcgg catggtgctc tacgcccagc ccatcgccga
    10381 ctgcctgcgg gctgtgcagg cccagtgcgc cgggcagggg cggcaaaagc cccatgtcgt
    10441 gttcctgacc gccgcaggcc gtccctataa cgaggaaaag gctcgcgagc tggcaagcta
    10501 tgacgcggtg accctcgtct gcggccacta tgagggcata gaccagcgcg tcattgacgc
    10561 cttcggcgat gaggaaatct ccatcggcga ctacgtgctg accggcggtg agctggcaag
    10621 ccttgtcgtg gccgacagcg tgctgcgcct gcagcccggc gttctggcag aggaaaaggg
    10681 ctaccaggac gagagctact gggatggact gctcgaatac ccgcagttta cccgccccga
    10741 agtgtgggag ggccgcgctg tgccgccggt gctgctgacc ggcgaccaca aaaaaatcga
    10801 cgaatggcgc ggtgcccaga gccgggagcg cacccgcgaa cgccgccccg acctgtacga
    10861 cgcctggtgc gagagtcacc cgctgacgga gcttcccaaa tggaagcgcg gcgaaaatat
    10921 gcgccttgtg aaaaatgacg aacagctggc cctctgcgcg gccctcatgg ctgagggccg
    10981 ccgcacagtt tgtgctccgg tctgcagcga ggaatatctt gcaaaaatga cgccggaatt
    11041 ctggctgccc gagctggcag acaaaaaaag gaacggctgg gcgttctatc tgcactacac
    11101 caaggatgcc gccgacggca tggtcggcgt ctgccataag accggtgagt tggggcactt
    11161 gtttgtgacc gagagtgccc ggggcaaggg atacggtgcc aagatgctgg attttgcccg
    11221 caagaagctg gcggagcacc cgcatccgac tttcggcgtt ttgtccacca acacccgcgc
    11281 cattgcgctg tacaaacgca tgggctggcg gctgaccggc accgtgctga atgtgtacga
    11341 cccggaaaac gacgcgttta cggaaaaata ctgcgaagaa tggcagatgc gctaccaggg
    11401 ctgaccgttg tttttgtcgc caatccacaa tgttgttgaa aataaactgg taaagttgca
    11461 caaacgcgcg ggcgcagatt tggcggaatt acagaatcct gccaaatttg caaaattcta
    11521 ctgtacaaaa tcgacgaaat aggataaaat agagacaatc aaggcggcgt attaacacac
    11581 gcaatagacc gcctggaagt cgcccacggt agatacgcaa gggcatatta ttttcaaaag
    11641 taggagagaa taactatgta cgttaaagaa gttaccgttg agaatcaggt tggtctgcac
    11701 gcacgtccgg ctaccttctt catccagaag gccaacgagt ataagtcctc catttgggtc
    11761 gagaaagagg agcgccgcgt caacgcgaag tctctgctcg gcgtcctgag cctgggcatc
    11821 gttggcggca ccaccatccg catcattgct gatggtgccg atgagcaggc tgcagtagac
    11881 ggcctggtca agctggtcag ctctggcttt gccgagtaat ctattccgga acaatcagcg
    11941 gtggggcgac ccgccgcttt ttttgtatac gaaaggctcc ctatgacaaa agctgaactc
    12001 tacaaaaaag cctgtatgct gccgcttttg cccggtgtct acatcatccg ggacaaaacg
    12061 gacaccatca tctatatcgg caaggccaag cggctcaaga cccgcgtgtc gcagtatttc
    12121 cgtgagggtg tgccccacga cgccaaggtc agccagatga ttgcccacgc cttcacgttt
    12181 gacgtgatcg tgtgtcaaag cgagtttgag gcactggtgc tggaagcaag ccagatcaag
    12241 gcccacacgc cgaaatacaa catcctgctc aaggacgaca aggggtacag ctatgtcaag
    12301 gtcacgcgtg gcgcgtggcc gcgcatctcc gccgcgctgc aaaaggacga tgacgacgcc
    12361 gactacatcg gccccttcac ctcgtccttt gccgtgcggg agatggtcga gaccgcgcag
    12421 gactgctttc tgctgccgcg ctgcaacaaa aacttcccgc aggatttccg caaggggcga
    12481 ccctgcctca acgcccacat cggcaagtgc atggccgtgt gcagcgggaa aattacctgc
    12541 gaagcctaca acgacgccgt gcagggcgca ctgcggatga tccgctacgg caaaaaggat
    12601 atcgtcaagc agctgcggga gaagatggaa gctgcgtccg aacgccttga ctttgagacg
    12661 gcggcgctgc tgcgcgacca gatcatggct attgaccgcg tggcggcagg gcagaaggtg
    12721 gtcatggaaa atgacaccga aatggacgtc atcgcgctgg cgggcacgac acacgcagtc
    12781 tgcgccgccg tgctgcgcta ccgtgacggc cgcctgaccg acaagcggga atttctcttc
    12841 cgcgacacga ccgatatcaa cgccgtgcgg gaagaatttt taccacagta ttacctggac
    12901 ggcggggcca tccccaagac gatcgcggtg gatgccttgc cgcccgacgc cgacgcgctg
    12961 agcgaagcgc tgaaccaggc ccgcggcaca aaagtggaac tctacgtgcc ccagcgcggc
    13021 gatgtagcca agctcgtgac gatggcctac accaacgccg tggagcgcct gggccgcgag
    13081 agcggccgct atacccgcga ggagaagctg ctggaagaag ccgcccaggt gctgggctta
    13141 aaaaagccgc cccgtgtcat cgagagctac gatatttcca actggggcga cgggtcgagc
    13201 gtctgcggca tggtggtgtt ccgggacggc aggccccacc gcgcgggcta ccgccgcttt
    13261 aaaatgaaaa ccgttgcggg cacggacgac tacgcctcgc tggcggagac gctctcccgc
    13321 cgcgcggcag agtacgagat ggcccgccgc ggcgaaaagc cggacggtcc gcagaaccag
    13381 ttcgcaacat tgcccgacct gctgctcatc gacggcggcc ggggccaagt gggcgcggtc
    13441 aagcaggctt tgcgcggtac ggcgctggag catgtaccga ccttcggcat ggtcaaggac
    13501 gaccaccacc gcacccgcgc catcgtggac gacgagggcg gcgaaatcgc catcaaccgc
    13561 caccgcaaca tttttacctt tgttacgaac atccaggacg agacgcaccg ctatgccaac
    13621 gactaccgca aacgcgccat gaagaaaaag tcctacgccg ccacgctcac ggccatcccc
    13681 ggtgtgggcg aaaagacgag cgccgcgctg ctggcacatt ttaaaactgt ctcggcggtc
    13741 aaagcggcca gtatttccga tttggaggaa gtcaaaggca tcagccacgc caaagcggaa
    13801 atactgtaca acgccctgcg gaatggggta taatgtaata acgatacaaa aatccaggat
    13861 ggtttcaaac ctatgctgct tctaacactt cttctttccc tcgccctcgc ggcctttttc
    13921 actttactgc ctgcccgcgt ccttcaccgt cgcaccaccc gcgccgacct gtacgtcggg
    13981 gcgggggtcg cggtgctggt ctggctgtgg gcggcctgtg tctggtccaa gcccgggttg
    14041 accgtcgact ttgacggctt ccgcctggcg gcgctgcttg ccatgcagct gctcgcctgc
    14101 ggggtggtca caggcttccg cctgcggcag atgctgccca ccaaactgcg caccccggcg
    14161 gcggtcggcc tgctggcggc agcagcactg gggctggaac ttttcgtcgg caacctcaac
    14221 tggcttgcca cccacggcta cacgcccatc gacctgcggc cctacctgac cgccgacgcc
    14281 gaccccaccg cgccgatcgt actgaacgac gagaacacaa cactgcaatt catcggcttg
    14341 gatttcccga tttataattt gcagctggac ggccttgtct cactggccga cggcgacacg
    14401 ccggagcaga aaaacgtgcg gctgaacctg aacgtttccg ctaccgatga agccgccaca
    14461 gtctcccgcc agagttggaa ctggccggtg gccgcggcgt cggcccgcag ctgggcccgc
    14521 agcctggatc tctccggcaa ggcagacagc ctgacgctga cggcctcggg ctttaacgga
    14581 gagtaccgga gctacccact gaacgcccag ctgaacaccg tttacgccaa cgcccgccgc
    14641 ccgctggatt tctctgtcct gcgtttcgcg gttgtgctgg cgctgctgct ggcgggcttt
    14701 gccctgcgcc cggcaagcgt cctctggcgc gacgcctacg ccgcccatga gcgcaaatac
    14761 cgcccggcgg tgctggctgt cgagctggcc ctctgcgcgg cggcgttcct ggcccccttc
    14821 ggcgaccgct tcaacgcggg cgtcgccacg agcttttaca acacgcccga ctggtcgggt
    14881 acgagccgca tcgacttcac catgcacatc aacgactggg cgtccaacac ggccgcccag
    14941 tacggcgcac tggctcacag ctttttgcag gggaggctcg atttggaaaa agacccgccc
    15001 gcggctatgg ctgaccttgc caacccctac gacaccgccg cccggcagga cgccgcgccg
    15061 gatgccctgt gggatgtggc ctactacaat ggccgctact acgtctattt tggcgttgta
    15121 ccgtgccttt tgttccagct gccctttgag gcactgaccg gcatccggga tctgccgcca
    15181 agcttgccga tgattttcct cgcctggctg tacatttttg cggtgttcgg tttcatcagg
    15241 caggccgtgc gccgctggtt cccgaacgcc agtgccgccg cctgcctgct gacagcggcg
    15301 ggcgcagcct cgggcagcca aatctactat ctgctgcacc gcccctcggt ctatgaatac
    15361 gccattttaa gcggtgctgc gtttgttctg ctggcactgt ggcagtggct ttgcgccgcc
    15421 aacgcgcccg aaacaaagcg caagaccatc ctgttccact tggccttcgg cagcctgtgc
    15481 atggcgctgg tggccggctg ccgcccgcag atggtgcttt ttgccgtgct ggccctgccg
    15541 attttacggc cgcgctatat catccaaaag cggctgcgca gccgcgccgg ggcgggggag
    15601 tgcgctgcat tcctgctgcc ggtcgtgctg gtggccgtga gccttatgtg gtacaacgcc
    15661 gcacggttcg gctcgccctt tgactttggt gccaactaca acctgaccag caatgatatg
    15721 acccgccgcg gttttgcggt ggggcgcatc gccccggcgg tcgtgacctt cttggcgggc
    15781 atccccggcg tgcagaccgt gttcccctat atcacggcca ccaaaatgca gacgaactac
    15841 atgggc
//
LOCUS       DBMDSQ010000017        15208 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_1232_length_15208_cov_2.264579, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000017 DBMDSQ010000000
VERSION     DBMDSQ010000017.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 15208)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 15208)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15208
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1232_length_15208_cov_2.264579"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1086)
                     /locus_tag="ACZPAX_01330"
     CDS             complement(<1..1086)
                     /locus_tag="ACZPAX_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005944102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type ATPase actuator domain-containing
                     protein"
                     /protein_id="HIR1112571.1"
                     /translation="MKWNILHESRGRMRLHLNKKRMSLAEADQLQDYLTALPGVRTAA
                     VYERTCDVVVVYTADRAGVVKGLAAFKFDVEALAEVPANSPRAVNREYQEKLVNMTIF
                     HFARKLFLPAPLRMAYTLVRSVPYVFRGLRCLWRRKLEVEVLDALSIGVSMLRGDFST
                     AGSVMFLLRLGELLEEWTRKKSLGDLARCMSLNVDRVWQQTAEGEVLVPISQVRAGDA
                     IVVHTGSVIPLDGKVLDGEASVNQASLTGEAEPVRKSNGAVVYAGTVVEEGQITVTVE
                     QQAGNGRYDQIVKMIENSEKLKSASETRAAALADKLVPYSLLGTAVTYALTRNVTRAI
                     SILMVDFSCALKLSMPLAVLSAMRECGS"
     gene            complement(1291..1557)
                     /locus_tag="ACZPAX_01335"
     CDS             complement(1291..1557)
                     /locus_tag="ACZPAX_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005944105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6110 family protein"
                     /protein_id="HIR1112572.1"
                     /translation="MNWAKIGLFAGGALFGSAGIELLTSKDAKKVYTQCTAAVLRMKD
                     QVMKTATSLQENCGDILADAKEINEQRAAEDDAVVEDAADKAAE"
     gene            complement(1990..3003)
                     /locus_tag="ACZPAX_01340"
     CDS             complement(1990..3003)
                     /locus_tag="ACZPAX_01340"
                     /inference="COORDINATES: protein motif:HMM:NF024878.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HIR1112573.1"
                     /translation="MANQTQRPDTALHNTSHLMQYHDLIESIVAAMDARDAYTAKHSD
                     RVADMVLVLATALDLSEDETTLLHMAAHLHDIGKIAVPDSVLRKAGPLTSPEWEEMRR
                     HPITGYEILRKVDDFKEIAVLVRHHHERWDGRGYPDGLRGADIPLGSRILAVADSIDA
                     MMSSRSYRPAMTSAACRREIERNSGIMYDPSVAAAALEHWDELVSRYAEADTPQTPYF
                     DRMQLEHTRQAHDYLLTHQGSRITLPELAARFHLSQSSLKICFKALYGVPVASYLRGL
                     RMDTAAQLLQESDLPVAEIAHRVGYEDPSRFAAAFRRHTGRRPTELRRVACPTVQRPA
                     SGV"
     gene            3506..4801
                     /gene="eno"
                     /locus_tag="ACZPAX_01345"
     CDS             3506..4801
                     /gene="eno"
                     /locus_tag="ACZPAX_01345"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015565412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HIR1112574.1"
                     /translation="MSYLEIEKVIGREIIDSRGNPTVEAEVWLADGTIGRGAAPSGAS
                     TGEFEALELRDGDKSRFGGKGVSKAVENINTTINEALVGMDASDIYAVDAAMLKADAT
                     KDKSNLGANAILAVSIAAARAAAISLDVPLYRFLGGVSGNKLPVPMMNILNGGAHAAN
                     TVDVQEFMIMPAGAPSFKEGLRWCTEVFHALQALLKSKGLATSVGDEGGFAPDLASDE
                     EAIQYILEAIEKAGYVPGKDFVLAMDAASSEWKGSKKGEYKLPKCGKVFTSEELVEHW
                     KRLVEKYPIYSIEDGLDEEDWEGWQHMTKELGGKVQLVGDDLFVTNTERLSKGISLGC
                     GNSILIKLNQIGSVSETLEAIKMAHKAGYTAIASHRSGETEDTTIADLAVALNTCQIK
                     TGAPSRSERVAKYNQLLRIEEELGGAAQYPGFGAFNIKK"
     gene            5025..7277
                     /gene="pflB"
                     /locus_tag="ACZPAX_01350"
     CDS             5025..7277
                     /gene="pflB"
                     /locus_tag="ACZPAX_01350"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005943931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HIR1112575.1"
                     /translation="MTDFKQWEGFEGRIWKEEVNTRDFIQKNYTPYDGDESFLAGPTD
                     ATNKLWGALQKLQKAERAKGGVLDMETEVVSGLTAYGPGYIDPELKDLEKVVGLQTDK
                     PLKRAFMPYGGIKMAEQACTTYGYQPSEKLHEIFTKYTRTHNQAVFDAYTPEMKKARH
                     SHIVTGLPDTYGRGRIVGDYRRVALYGVDYLIKAKQNDFANCGDGTMTEEVVRQREEI
                     AMQISALKGMKEMAAAYGFDISQPAANAKEAVQWLYFGYLAAIKTQNGAAMSVGRVST
                     FLDIYIQRDLDNGTLTETEAQELIDHMVMKFRMVKFARIPSYNQLFSGDPVWATLEVG
                     GIGMDGRSMVTKNDFRFLHTLENMGPAPEPNMTVLYSSALPKTFKDYASKISIDTSSV
                     QYENDDVMKPVWGDDYSICCCVSATQTGKEMQFFGARANLAKCLLYAINGGVDEKLGE
                     QVGPAYAPITAEYLDYNEVMAKYDVMMDWLAGLYVNILNLIQYMHDKYYYEAAEMALI
                     DTEVRRTFATGIAGFSHVVDSLSAIKYAKVKCIRNEQGLVTDYEIEGDFPKYGNDDDR
                     ADDIAVELLRSFLEKIKRRHTYRNSEPTTSILTITSNVVYGKATGAMPDGRKAYTPFA
                     PGGNPSYGAETHGLLASLNSVAKLPYHWALDGISNTQTINPDALGHSEGERVTNLVQV
                     MDGYFDQGAHHLNVNVFGTEKLLDAMEHPEKEEYANFTIRVSGYAVKFIDLTKEQQLD
                     VIARTCHKVL"
     gene            7586..8347
                     /gene="pflA"
                     /locus_tag="ACZPAX_01355"
     CDS             7586..8347
                     /gene="pflA"
                     /locus_tag="ACZPAX_01355"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HIR1112576.1"
                     /translation="MSETLGYIHSVETFGLVDGPGVRYIIFLQGCAMRCQYCHNPETW
                     AFTKDTAKTPQEAFTAAYRYRNYWRSNGGLTISGGEPLLQMDFVSEVFRLARAKKVQT
                     ALDTSAQPFAPDNADWMARFDRLLENTDLVILDLKEMDDEKHKKLTGHSNKNILAMAQ
                     YVASKGVHLWIRHVLVPGLTDDADGLRALDAFIKTLPTVRRVEILPYHTLGLFKWQNL
                     GIPYPLDGVRVPTAEEIAAAEQLLHVQDYPDAPKG"
     gene            complement(8868..9857)
                     /locus_tag="ACZPAX_01360"
     CDS             complement(8868..9857)
                     /locus_tag="ACZPAX_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008680803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR1112577.1"
                     /translation="MYQASQNRYCVLPYRRCGDSGLLLPAVSLGLWHNFGDTAPYENM
                     RALCRTAFDNGITHFDLANNYGPEPGAAERNFGKILRDDLGVYRDELIISTKAGFEMW
                     DGPYGNWGSRKYLLASLDQSLRRMGLDYVDIFYHHRMDPNTPLEETMGALAQAVRSGK
                     ALYVGLSNYDGPTLEKAAAILDELHVPFVINQNRYSIFDRTVENNGLKETAAKLHKGI
                     ITFSPLAQGLLTNRYLDGIPADSRVHTDGRFLKEKDITPEKIAKIRALNEIAAARGQT
                     LAEMALAWLLSHEEITTVLIGASKTQQILDNIKAVQNMKFTTEELAEIDRISK"
     gene            10017..10475
                     /locus_tag="ACZPAX_01365"
     CDS             10017..10475
                     /locus_tag="ACZPAX_01365"
                     /inference="COORDINATES: protein motif:HMM:NF024212.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIR1112578.1"
                     /translation="MQEKQRQVYDLLEITNRQFNECNALYHAVAQRYGLSDAAFWVLY
                     ALRTTHEPQTQNRMAAEWGQPKQTLNSAVAAMVKKGLVELCPGNGAHSGKIVTLTDEG
                     RALAARTVEPVIAAEQQAMTRQGLAEVEQNCRLTQRYLECLRQEFEKIAR"
     gene            10575..11921
                     /locus_tag="ACZPAX_01370"
     CDS             10575..11921
                     /locus_tag="ACZPAX_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005937496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1112579.1"
                     /translation="MSKSNRIQLSDHFTTGRLLKFTASPIIMMIFTSIYTIVDGFFVS
                     NYAGKTAFTALNLIYPYLQMLGCLGFMIGTGGSALVAMTLGAGDEKRANRLFSMLAVA
                     TAVLGVLFSAFGLALLEPVAVLLGATPELLPSCLLYGRILLTFQTAFMLQYLFQSFFI
                     AAEKPKLGLFFTVAAGATNMVLDFVLVGVAGLGLAGAAAATVISQMVGGVAPIFYFIK
                     RRPGCRLYFTKPLFSLRELFKACTNGASELMTNISMSLVSALYNIQLLKLFGADGVAA
                     YGVLMYVGFVFAAVFIGYSQGTAPIVSYHFGADNKDELKNLLRKSAGIIAVAGVAMLA
                     SSELLAGPLAKIFVGYDAGLLALTIHGMKLYSISFILSGFNIFGSAFFTALNNGAVSA
                     AISFLRTLLFQIVSILTLPLLIGVDGIWLAVVAAETMALVCTAGFVVWGRRKYGYM"
     gene            complement(12176..12748)
                     /locus_tag="ACZPAX_01375"
     CDS             complement(12176..12748)
                     /locus_tag="ACZPAX_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007048258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ANTAR domain-containing response regulator"
                     /protein_id="HIR1112580.1"
                     /translation="MAKALIVSAGNNANEYLARHIGELGYTRPTIVASGGEARRRMDS
                     NDFEVVIINTPLPDEFGHELGTAAVEKTDAGVILLAKTGTAEQIADKLQDFGVLVLGK
                     PFTAIQFRQAVQIAASNYKRLAVLRAENAKLLDKIAQLRLVDRAKCYLIEKKGMTETE
                     AHRLIEKGAMDTRRSRGEVAQEILEDEEEE"
     gene            complement(12826..14136)
                     /locus_tag="ACZPAX_01380"
     CDS             complement(12826..14136)
                     /locus_tag="ACZPAX_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007048257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HIR1112581.1"
                     /translation="MKRTAQDILNFVEDNDVKFAKLTFCDIFGNQKNVSLFASELPRA
                     FAEGVSFDGSSIAGFMNVEESDLVLWPDPDTATVLPWRPTEGRVIRMYCDITLPNGKP
                     FEGNCRGYLQSVVKRARAMGLTCNVGCECEFYLFETDENGNPTRIPLDMGGYFDIPPL
                     DKGENIRREICFAIEEMGLHPEHSHHESGHGQNEVCFRYTTALKSADNLNTFKSTVKA
                     IAARNGLFASFMPKPLANQPGSGLHVNVSLLRDGKNLFDGDFTPDSEAGHFVAGILAH
                     ARELTAFCNPVPNSYARLGANEAPLYVSWSRQNRSQLVRLPSAHGEFCRVELRGPDPA
                     GNPYLVIGLILAAGLDGIQRKLPLPEAVNRNLFHPSAAAGLESLPHTLREAITVAGQS
                     EFISAELPVALQNKYFEYQTQLCHDAESAPDTDSWERAHSFLII"
     gene            complement(14392..>15208)
                     /locus_tag="ACZPAX_01385"
     CDS             complement(14392..>15208)
                     /locus_tag="ACZPAX_01385"
                     /EC_number="6.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007048255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine synthetase III family protein"
                     /protein_id="HIR1112582.1"
                     /translation="SKDTTDRNRTSPFAFTGNKFEFRMPGSAENLSDANTILNTAVAK
                     SLKEFVAETADAADFECAAAAWVKKTLNAHRRVIFNGNGYSDAWEQEAERRGLPNRKC
                     TPDAMIALKDDKNIALMEEFGVLTKTEMLSRYEVEMEHYSKILNIEARTMLKIANKQL
                     IPAATAYMGEVASSAAAKTAAVEGISTKAETKVLTRLSKYTDEMSDAADTLTEVTDKV
                     SAMDDEAAKAHAFHDEVIPAMDALRAAADEAESLVDEDYWPLPCYSRMLFYTE"
BASE COUNT         2961 a         4499 c         4657 g         3091 t
ORIGIN      
        1 gctgccgcac tcacgcatgg cggacagcac ggccagcggc atagacagct tgagcgcgca
       61 ggagaaatcg accatcagaa tcgagatcgc acgggtcacg ttgcgggtca gcgcgtaggt
      121 cacggcggtg cccagcagac tgtacggcac cagcttgtcc gccagggccg cagcgcgggt
      181 ctcgctggca gacttcagct tctcggagtt ctcgatcatc ttgacgatct ggtcatagcg
      241 gccgttgccg gcctgctgct ccacggtcac ggtgatctgg ccttcctcga cgacggtgcc
      301 ggcgtagacg acggcaccgt tcgacttgcg cacaggctcg gcctcaccgg tcagcgatgc
      361 ctggttgacg ctggcctcgc cgtccagcac tttgccgtcc agcgggatga cactgccggt
      421 gtgtacgacg atggcgtcgc ctgcgcggac ctgggaaatc ggcaccagca cttcgccctc
      481 ggcggtctgc tgccagacgc ggtcgacatt cagcgacata cagcgggcca ggtcgcccag
      541 ggacttcttg cgggtccatt cttccagcag ttcacccagg cgcagcagga acatgaccga
      601 cccggcggtc gagaagtcgc cgcgcagcat cgaaacaccg atggacagcg cgtcgagcac
      661 ttcgacttcc agcttgcgcc gccacaggca gcgcagaccg cggaaaacgt agggcacgct
      721 gcgcaccagt gtgtaggcca tgcgcagcgg tgcaggcagg aacagcttgc gcgcaaagtg
      781 gaagatcgtc atattgacga gcttttcctg atattcgcgg ttgacggcgc gggggctgtt
      841 ggccgggact tcggccaggg cctccacatc gaacttgaaa gcggccagcc ccttgacgac
      901 cccggcgcgg tcagcggtgt agacgaccac gacgtcacag gtgcgctcat acacggcggc
      961 ggtgcgcacg ccgggcaggg cagtcaggta gtcctgcagc tggtctgcct cggctaaaga
     1021 catccgcttt ttgttgaggt gcaggcgcat ccgcccgcgg ctctcgtgca gaatattcca
     1081 tttcataagc tactctccca agaaataaaa aaggcttccc cggtaggtgg ggaaggcttt
     1141 tttagcaagc gttaaaacgc agtagaggtg taggggcgca tatatgcgcc ccgcagccta
     1201 cccgctagta gtcgtccgcc gtgtggttgc gggcggccta tatgccgccc ctacatggcg
     1261 tcggctgcat ttgcagtaat ctgtcaaaaa ttactcggcg gccttgtcgg cggcgtcctc
     1321 aacaacagcg tcatcctcgg cggcgcgctg ctcgttgatc tccttggcgt cggccaggat
     1381 gtcaccgcag ttctcctgca ggctggtggc ggtcttcatg acctggtcct tcatgcgcag
     1441 cacggcggcg gtgcactggg tgtacacctt cttcgcgtcc ttgctggtca ggagctcaat
     1501 accggcggaa ccaaacagtg cgccgcctgc aaacaaacca atctttgccc aattcatagt
     1561 tcaaatcccc tttacaaaaa gttatggtaa taccgcataa ttctaagtgc cgatacggcg
     1621 agcgaggtgc ggcagctgct aagccaaaag cgcagataat actttgtgct gcaaaggtga
     1681 gcgccgccag tggcggatgc agcgaaccga agcaggggca gcggtcgcag agtgcgaggg
     1741 gcttttgccc cgaagcacga tgcgggtacc gcaacccgac attatcgagc attttggcaa
     1801 cgcagatgcc gtgccgcagc cgccggagcg gtgcttaagc cgtcaggcgg gaattgtgcg
     1861 gtgttgcctt atataaattg tgccctaaag ggcgaacctg taaggtttaa gcataatttt
     1921 acaacagtta gttatgactg agttagacca aaaagacggg tgatgtgcag attggatttt
     1981 atgtgtggat cacacaccgc tggcgggccg ctggaccgtc gggcacgcta cccgccgcag
     2041 ctctgtgggg cggcggccgg tgtggcggcg gaaggccgcg gcaaaccggc tggggtcctc
     2101 gtagccgaca cggtgcgcga tctcggccac cggcaggtcg ctctcctgca aaagctgggc
     2161 ggcggtgtcc atgcgcagcc cgcgcaggta gctggccacc ggcacgccgt acagcgcctt
     2221 aaagcagatc ttcagcgagc tttgggaaag atggaaccgc gcggccagct cgggcagggt
     2281 gatacggctg ccctggtggg tcagcaggta gtcgtgggcc tggcgggtgt gctccagctg
     2341 catccggtca aagtagggcg tctggggtgt gtcggcctcg gcatagcggc tgaccagctc
     2401 gtcccaatgc tccagcgcgg cggcggccac gctggggtca tacataatgc cgctgttgcg
     2461 ttcaatttcg cggcggcagg cggcgctggt catggccggg cggtaactgc ggctgctcat
     2521 catggcgtcg atggaatccg ccaccgccag aatgcgcgac cccagcggga tatcggcgcc
     2581 gcgcaggccg tctgggtagc cgcggccgtc ccaccgctca tgatggtggc gcaccagcac
     2641 ggcgatctct ttgaaatcat cgaccttgcg cagaatttca tacccggtga tggggtggcg
     2701 gcgcatttcc tcccactcgg ggctggtcaa aggcccggcc ttgcgcagca cggaatccgg
     2761 cacggcaatc ttgccgatat cgtgcaggtg ggcggccatg tgaaggagcg tcgtctcatc
     2821 ctcgctcaaa tccagggcgg tggccagcac cagtaccatg tcggctaccc ggtcggagtg
     2881 cttggcggtg taggcatcgc gggcgtccat ggcggcgacg atgctctcga tcaggtcatg
     2941 atattgcatc agatggcttg tattgtgcag cgcggtgtcc ggccgctgtg tctggtttgc
     3001 catagccgcg tctcccttcc agtagttgtg gttagtatgg attaactgtt agtttagact
     3061 atcttactgc gcggcgcaaa atttgtcaag tgcgcggcaa acacattgga aaaagagtgt
     3121 ggttgtgata caatgcccgg cctgcgacct tgaggcggct gcccatttat ctgttgccat
     3181 tgtagggcgg ggtcttgacc cctccggggc ggcctggggg caggccgccc tacaatctaa
     3241 gaggaaaccg catgccaact tgcggggcgt aggggacgcc gccccctaca actagcgtga
     3301 tacagggcaa aatgttcgct gcgccaaatt tactatcggt ttcagctgtc tgatgtgccg
     3361 gacggctgtt tttttatacc ccttgacaaa tgcggttagt ctatgctatc ttatggctgg
     3421 ttagaaagca ctaaccaagt ggtatcgctt tgtccccttc cgggcatact gtaaaaagta
     3481 ttttatagag aaaggacttt gcatcatgag ctatctggaa attgagaaag tcattggccg
     3541 cgagatcatc gactcccgcg gcaaccccac tgttgaggcc gaggtttggc tggcggacgg
     3601 caccatcggc cgcggtgctg cccccagcgg cgcttccacc ggcgaatttg aggccctgga
     3661 gctgcgcgac ggcgacaaga gccgtttcgg cggcaagggc gtcagcaagg ccgtggagaa
     3721 catcaacacc accatcaacg aggcccttgt cggcatggac gcctctgaca tctacgccgt
     3781 cgatgccgct atgctgaaag ccgacgccac caaggataag tccaacctcg gcgcaaacgc
     3841 catcctggcc gtctccatcg ccgcggcccg cgcagccgcc atctctctgg atgtgcccct
     3901 gtaccgtttc ctgggcggcg tgtccggcaa taagttgcct gtgcccatga tgaacattct
     3961 gaacggcggc gcacacgcgg ccaacaccgt ggatgtgcag gaattcatga tcatgcccgc
     4021 cggtgccccc agcttcaaag agggcctgcg ctggtgcacc gaggtgttcc acgccttgca
     4081 ggcactgctg aagagcaagg gcttggctac ctccgtcggt gacgagggcg gctttgcgcc
     4141 tgatttggcc agcgacgagg aggccatcca gtatatcctc gaggctattg agaaggccgg
     4201 ttacgtgccc ggcaaggact tcgtcctggc tatggatgcc gcctccagcg agtggaaggg
     4261 aagcaagaag ggcgagtaca agctgcccaa gtgcggcaag gtctttacca gtgaggaact
     4321 ggtcgagcac tggaagcggc tggtcgagaa atatccgatc tactccatcg aggacggcct
     4381 cgacgaggag gactgggaag gctggcagca catgaccaag gagctgggcg gcaaggtgca
     4441 gctggttggc gacgacttgt ttgtcaccaa caccgagcgc ctgtccaagg gcattagcct
     4501 gggctgcggc aactccatcc tcatcaagct gaaccagatc ggttctgtct ctgagacgct
     4561 ggaggccatc aagatggccc acaaggccgg ttacaccgcc attgcctccc accgctccgg
     4621 cgagaccgag gacaccacca ttgccgacct ggccgttgcg ctgaacacct gccagatcaa
     4681 gaccggtgcc cccagccgca gtgagcgcgt tgccaagtac aaccagctgc tccgcatcga
     4741 ggaagagctt ggcggcgctg cgcagtaccc gggatttggg gcgtttaata ttaaaaagta
     4801 aataacaggg aaaaatcttc gggcttgaca aaaagcgtgc ggtaggggtg gagcagagcc
     4861 ccgcccctac gcatctgtag aaacaatcgc gcgtttttgg cgcaagaagc tttgtaaaat
     4921 ttctgtaatg ttgccgagac aacatacgca aaccctgatt ttttggtata gtatctttgc
     4981 cgggtgggaa aacccggctg tccataactt aaaagaggtg cataatgaca gatttcaaac
     5041 agtgggaagg ctttgaaggc cgcatctgga aagaggaagt caacacgcgt gacttcatcc
     5101 agaagaacta caccccttat gacggcgatg agagcttcct cgccggcccg accgacgcta
     5161 ccaacaagct gtggggcgct ctgcagaaat tgcagaaggc cgagcgcgcc aagggcggcg
     5221 ttctggacat ggagaccgaa gtcgtctccg gtctgaccgc ttacggcccc ggctacatcg
     5281 accccgagct gaaggacctt gaaaaggtcg tcggcctgca gaccgacaag cccctaaagc
     5341 gcgccttcat gccctacggc ggcatcaaga tggcggaaca ggcctgcacc acctacggct
     5401 accagcccag cgaaaagctg cacgagattt tcaccaagta cacccgcacc cataatcagg
     5461 ctgtgtttga tgcctacacc cccgagatga agaaggcacg ccacagccac atcgtcaccg
     5521 gtctgcccga cacctacggt cgcggccgta tcgtcggcga ctaccgccgc gtagccctgt
     5581 acggcgtcga ttacctcatc aaggccaagc agaacgactt tgccaactgc ggcgacggca
     5641 cgatgaccga ggaggtcgtc cgccagcgcg aagaaattgc aatgcagatt tctgccctga
     5701 aaggcatgaa ggagatggct gccgcctacg gcttcgacat ctcccagcct gctgccaacg
     5761 ccaaggaggc tgtccagtgg ctgtacttcg gctatctggc cgccatcaag acccagaacg
     5821 gcgcggctat gtccgtcggc cgcgtgtcca ccttcctgga tatctacatc cagcgcgacc
     5881 tcgacaacgg caccctgacc gagaccgagg cgcaggagct gatcgaccac atggtcatga
     5941 agttccgtat ggtcaagttc gcccgcatcc ccagctacaa ccagctgttc agcggcgacc
     6001 ccgtctgggc gaccctcgag gtcggcggca tcggcatgga tggccgctcc atggtcacca
     6061 agaacgactt ccgcttcctg cacaccctcg agaacatggg ccccgccccc gagccgaaca
     6121 tgacggtgct gtactccagc gccctgccca agaccttcaa ggattatgcg tccaagatct
     6181 ccatcgacac ctccagtgtc cagtatgaga acgacgatgt catgaagcct gtctggggcg
     6241 atgactactc catctgctgc tgcgtttctg ccacccagac cggcaaggag atgcagttct
     6301 tcggtgcccg cgcgaacctt gccaagtgcc tgctgtacgc catcaacggc ggcgtcgatg
     6361 agaaactcgg cgagcaggtc ggccccgctt acgcgcccat caccgctgag tatctcgact
     6421 acaatgaggt catggccaag tacgatgtca tgatggactg gctggctggc ctgtacgtca
     6481 acatcctgaa cctcatccag tatatgcacg acaagtacta ctatgaggct gccgagatgg
     6541 ccctgatcga caccgaggtc cgccgcacct tcgcgaccgg cattgcgggc ttcagccatg
     6601 tggtcgactc tctgtccgcc atcaagtatg ccaaggtcaa gtgcatccgc aatgagcagg
     6661 gcctcgtcac cgattacgag atcgagggcg acttcccgaa gtacggcaac gatgatgacc
     6721 gcgccgacga tatcgccgtc gagctgctgc gcagcttcct ggaaaagatc aagcgccgcc
     6781 acacctaccg caactctgag ccgacgacct ccatcctgac catcacctcc aacgtcgtct
     6841 acggcaaggc caccggcgct atgcccgacg gccgcaaggc atacactccg tttgcccccg
     6901 gcggcaaccc gagctacggc gctgagaccc acggcctgct ggcgtccctc aactccgttg
     6961 ccaagctgcc ctaccactgg gcgctggacg gcatctccaa cacccagacc atcaaccctg
     7021 atgccctggg ccacagcgag ggcgagcgcg tgaccaacct tgtgcaggtt atggacggct
     7081 actttgacca gggtgcccac catctgaatg tcaacgtctt tggcaccgag aagctgctgg
     7141 acgccatgga gcaccccgag aaggaggagt acgccaactt caccatccgt gtctccggct
     7201 acgcggtcaa gttcatcgac ctgaccaagg agcagcagct ggatgttatt gcgcgtacct
     7261 gccataaggt cctgtgattt tctaagctga ataaggcccg cagctgcggc tgcgggcttt
     7321 tttgtggtat actgtgttat accacccggt aggggccggg catgcccggc ccgcaggttt
     7381 acggcgctgc ccctttcacg gttcgcatcg cagggagggg tcttgaccct ccgcggcggc
     7441 cagagggctg gccgccctac aagcgcccgc aaaaggaatg cgtaggggag ggatttatcc
     7501 ctcccgggac gtgtgcggca gccgcggggc gtcgaggacg ccgcccccta ccaattcatt
     7561 ttcccgataa acggaggcac actttatgtc cgaaacctta ggctacatcc attctgttga
     7621 gaccttcggc cttgtcgatg gccccggcgt gcggtatatc atctttttgc aggggtgtgc
     7681 catgcgctgc caatactgcc acaaccccga gacatgggcc tttaccaaag ataccgccaa
     7741 gaccccgcag gaggccttta ctgccgccta ccgctaccgc aactactggc gcagcaacgg
     7801 cggcctgacg atcagcggcg gcgagccgct gctgcagatg gattttgtca gcgaggtgtt
     7861 ccggttggca cgcgctaaaa aggtgcagac cgcgctggac accagcgccc agccctttgc
     7921 gcccgacaat gccgactgga tggcccgctt tgaccgcctg ctggaaaaca ctgacctcgt
     7981 cattctggat ttgaaagaaa tggacgatga aaagcacaaa aagctgaccg gccacagcaa
     8041 caaaaatatt ctggcgatgg cgcagtatgt ggcgtccaag ggcgtgcatt tgtggatacg
     8101 ccatgtgctg gtgcccggcc tgaccgatga cgccgacggc ctgcgcgcgc tggatgcatt
     8161 cattaaaacg ctgcccaccg tgcgtcgggt ggaaattctg ccctaccaca cgctggggct
     8221 gttcaagtgg cagaatttgg ggattcccta tccgctggac ggcgtgcgcg tgcccacggc
     8281 ggaggaaatc gctgcggcgg agcagctgct gcacgtacag gattaccccg atgccccgaa
     8341 aggctaattt ctacaaaaat agtgggaatt atttttgaaa aacccttgac accagcgctt
     8401 ccatatggta aagtatactc gtggttagga actgctaacc ataaccaacc tcgaaccgtc
     8461 aaccggctgt ttgcctctcc ggtacggttc ctgccaataa caggctgcgc ctccgttttg
     8521 gcgttttgct ctgcgccttt ttagcggtct gcttattgcg ttttgccctc atcgttctct
     8581 gctccggacg atgggggatt ttttattgat gtacccccct tagagggcgc accaataaaa
     8641 aacaaccgcg tacagcggtt gttccgctta cacccgcccg cgaacgcggg caaggaaagg
     8701 agacggcccg gcccgcaagc ttgcgccaaa tccctttttg cggttcgcat tgtaggggag
     8761 aaattcatcc ctcccgggca cttcccgctg ccgcaaacga ccacggcggc cagaggactg
     8821 gccgccctac aacaaaaacg gcttcccctt gggggggaag ccgttttcta tttgctgatc
     8881 ctgtcgatct ccgccaattc ttcggttgta aacttcatgt tctgcacggc cttgatgtta
     8941 tccaaaatct gctgcgtctt ggacgcgccg atcaaaacgg tcgtgatctc ctcgtggctc
     9001 agcagccagg ccagcgccat ttcggccagg gtctggccgc gggcggcggc gatctcattc
     9061 aacgcgcgaa tctttgcgat tttttccggt gtgatgtcct tctccttcaa aaaacgtccg
     9121 tcggtgtgta cgcggctgtc ggccgggatg ccgtccaggt agcggttcgt cagcaagccc
     9181 tgcgccagcg ggctgaaggt gatgatgccc ttgtgcagct tcgcggcggt ctctttcagc
     9241 ccattgtttt ccaccgtgcg gtcgaaaatc gaatagcggt tctggttgat gacgaacggc
     9301 acgtgcagct cgtccagaat ggccgctgcc ttttccagtg tcgggccgtc gtagttggac
     9361 aggcccacat acagcgcttt gccgctgcgc acggcctgtg ccagcgcacc cattgtctct
     9421 tccagcgggg tgttcggatc catacggtgg tggtagaaaa tgtccacata gtccagcccc
     9481 atgcggcgca ggctttggtc aaggctggcc aacaggtact tgcggctgcc ccagttgccg
     9541 taggggccgt cccacatctc gaacccggct ttggtggaga tgatcagctc gtcgcggtag
     9601 acgccgaggt cgtcccgcag aattttaccg aaattccgct cggccgcgcc cggctcgggg
     9661 ccgtagttgt tggcaaggtc aaaatgggtg atgccgttgt cgaacgccgt gcggcagagc
     9721 gcgcgcatat tttcgtaggg tgccgtgtcg ccaaaattat gccacagccc cagtgagacg
     9781 gcgggcagca gcaggccgct atcgccgcag cggcggtaag gcaggacaca gtagcggttc
     9841 tgagatgcct gatacatggt aaaaaccccc ttttatactc ctattataaa agcagaaaaa
     9901 cggacacaca atggcaaagg tgtacaaaaa actgcgttaa aatttgtagg cttgacataa
     9961 atccgatttc ggattataat aaaaatccga aatcggattc tgaaaagagg tgccctatgc
    10021 aggagaaaca gcggcaggta tatgatctgc ttgaaataac gaaccgccag ttcaatgagt
    10081 gcaacgctct gtaccacgcg gtggcgcagc ggtacggctt gtcggacgca gcattctggg
    10141 tgctgtatgc gctgcgcact acgcatgagc cgcagaccca gaaccgtatg gcggcagagt
    10201 gggggcagcc caagcagacg ctgaactctg ccgttgctgc catggtgaaa aaggggctgg
    10261 tggagctttg cccgggaaat ggcgcacaca gcggtaagat cgtgaccctg acggacgagg
    10321 gccgtgccct ggccgcccgc acggtggagc cggtcatcgc agccgagcag caggccatga
    10381 cccgtcaggg tcttgctgag gtcgagcaga actgccgact gacccagcgg tatctggaat
    10441 gcttacggca ggagtttgaa aaaattgcgc ggtaggggcc gggtatgccc ggcccgcagg
    10501 ctgtcgaaaa acgcatgttt gggcgaccaa tggtcgcccc tacaagcgtg tttaccgtaa
    10561 aaacaggagc atttatgtca aaatcaaacc ggatccaatt atctgaccac tttaccaccg
    10621 ggcggctgct gaaattcacg gcatcgccca ttatcatgat gatctttacg tcgatttaca
    10681 ccatcgtgga tggcttcttt gtctcgaact atgcgggtaa aacggcgttt acggcgctga
    10741 acctcatcta cccgtatctt cagatgctgg gctgcctcgg ctttatgatc ggcacgggcg
    10801 gcagcgccct tgttgccatg acgctgggcg ccggcgatga aaagcgcgca aaccggctgt
    10861 tcagtatgct ggcggttgca accgccgtgc tgggtgtgct gttcagcgct ttcggtcttg
    10921 cactgctgga accggtagcc gtgctgctgg gcgcaacgcc ggaactgctg cccagctgcc
    10981 tgctgtatgg ccgcattctg ctgacgttcc agacggcgtt catgctgcaa tacctgttcc
    11041 aaagcttctt tattgccgcg gaaaagccca agctgggcct gttcttcacg gtggcagcgg
    11101 gtgcgaccaa tatggtgctg gacttcgtcc ttgtaggtgt ggcggggctg ggcctggccg
    11161 gcgcggcggc ggctaccgtc atcagccaga tggtcggcgg cgtggccccc atcttctact
    11221 ttatcaagcg acgccccggc tgccgcttat atttcaccaa gccactgttc agtctgcggg
    11281 agctgttcaa ggcctgcaca aacggcgcga gcgagctgat gacgaacatt tcgatgtcgc
    11341 tggtcagcgc actgtataac atccagctgc tcaagctgtt cggtgcggac ggcgtcgcgg
    11401 cctacggcgt gcttatgtat gtgggctttg tgttcgcggc ggtgttcatc ggctattctc
    11461 agggcacagc gcccattgtc agctaccact ttggcgcgga caataaggac gaactgaaaa
    11521 atctgctgcg caaaagtgcg ggcatcattg cggtggccgg ggtggcgatg ctggcttcca
    11581 gtgagctgct ggccgggccg ctggcaaaaa tctttgtcgg ctacgatgcg ggcctgctgg
    11641 cgctgacgat acacggcatg aagctgtact ctatcagctt tattctgtcc gggttcaaca
    11701 ttttcggctc ggcgtttttc acggcgctga acaacggcgc ggtgtcggcg gccatcagct
    11761 ttttgcgcac gctgctgttc cagattgtgt cgattttgac cctgccgctc ctcattggtg
    11821 tggacggcat ctggctggcg gtcgtggccg ctgaaaccat ggcgctggtg tgtacggcgg
    11881 ggtttgttgt gtggggacgc cggaaatatg ggtatatgta aaaaagagga cgcgaaagcg
    11941 tcctcttttt tacgccaaaa ggcttccccc ctcggggctg cgcccgcagg cgcgtgtcga
    12001 agcgcaaccg ccggaggcgg ctcttagcgc ggagactgaa gctgccgcaa agcggacgga
    12061 tgtggggcga gcttgccttg ttgccctttt gcggctcaat atcggcacac tcgcccctca
    12121 tccggcctcg ggcggggcct cggccacctt cccccgcggg ggaaggctac ctttcttatt
    12181 cctcctcctc atcctccaaa atctcctgcg cgacctcgcc gcggctgcgg cgggtatcca
    12241 ttgcgccttt ctcgatgagg cggtgggcct cggtctcggt catgcccttt ttctcgatca
    12301 gatagcactt tgcgcggtca acgagccgca gctgggcgat cttatccagc agcttggcgt
    12361 tctcggcgcg cagcacggcc aaacgcttgt agttgctggc cgcgatctgt acggcctgcc
    12421 ggaactggat cgccgtgaac ggcttgccca gtaccagcac accgaaatcc tgcaatttgt
    12481 cggcgatctg ctcggcggtg ccggtcttgg ccagcagaat gacgccggcg tcggttttct
    12541 cgacggcggc ggtgcccagc tcatggccga actcgtcggg caggggcgtg ttgatgatga
    12601 cgacctcaaa gtcgttggaa tccatgcggc ggcgtgcctc gcccccgctg gcgacaatgg
    12661 tggggcgggt gtagcccagc tccccgatat gccgcgctaa atattcattg gcgttgttcc
    12721 cggccgaaac gatcaatgct ttggccatgg gccgccctcc cttcctgata aatttttgtg
    12781 cctgccgtgt gcgggcggcg gaaaatttcc gggtgggtcg tagggtcaaa taatcaggaa
    12841 gctatgcgct ctttcccagc tgtcggtgtc gggggcagat tcggcgtcgt ggcagagctg
    12901 ggtctggtac tcaaaatatt tgttttgcag cgcgaccggc agctcggcgc tgataaactc
    12961 gctctggcct gctacggtga tggcctcgcg cagggtgtga ggcagacttt ccagaccggc
    13021 agcagcgctg gggtggaaca ggttgcggtt cacggcctca ggcaggggca gcttgcgctg
    13081 gatgccgtcc aggccggctg ccagaatcag cccgatgacc agatacgggt tgcccgcagg
    13141 gtccgggccg cgcagctcca cgcggcagaa ctcgccgtgg gcgctgggca gccgcaccag
    13201 ctggctgcgg ttctggcggc tccagctcac gtagagcggg gcctcgttgg cacccagccg
    13261 cgcgtaggag ttggggaccg ggttgcagaa ggccgtcagc tcccgtgcgt gggcaaggat
    13321 gcccgccaca aaatgcccgg cctcgctgtc gggggtgaaa tcaccgtcaa acaggttctt
    13381 gccgtcccgc agcagcgaga cattgacgtg gaggccgctg cccggctggt tggcaagggg
    13441 cttgggcata aagctggcaa acaggccgtt gcgggccgca atggctttga ccgtgctctt
    13501 gaaagtattc agattatcgg cggatttcag cgccgtggtg tagcggaagc agacttcgtt
    13561 ctggccgtgg ccgctctcgt ggtggctgtg ctcggggtgc aggcccattt cctcgatggc
    13621 aaagcagatc tcccggcgga tgttctcgcc cttgtccagc ggcggaatgt caaaatagcc
    13681 gcccatatcc agcgggatgc gggtggggtt gccgttctcg tccgtctcaa acagatagaa
    13741 ctcgcactcg cagcccacat tgcaggtcag gcccatggcg cgggcgcgct tgacgacgga
    13801 ttgcaggtag ccgcggcagt tgccctcgaa cggtttgccg ttgggcagcg tgatatcgca
    13861 gtacatacgg atgacgcggc cctcggtagg ccgccagggc agcacggtgg cggtgtcagg
    13921 gtcgggccag agcaccaggt cgctctcttc cacgttcatg aacccggcga tggacgagcc
    13981 gtcgaagctg acgccctcgg caaaagcgcg gggcagttca ctggcaaaga gggaaacatt
    14041 tttctggttg ccgaagatat cgcagaacgt cagtttggca aacttgacgt cgttgtcctc
    14101 aacaaaattg agaatgtcct gtgcggtgcg tttcataggg gacctcctgt aaaagccttc
    14161 ccccaagggg gaagccacac atactcgaaa ggcccacctt gtgcgaggcg ggccttccga
    14221 gctggtgtaa gaaataggaa aaggagaatg gcgtgatgtg ggcgggctgc tgcttgcggg
    14281 aaagcagccc atcccgtagg ggcgcattct atatgcgccc gtcaacaggc agccccttgc
    14341 gggcggatat ggaatccgcc cctacagagc cgcgcggaga atgcccttac attactccgt
    14401 atagaacagc atacgagagt agcagggcag cggccagtaa tcctcgtcga ccaggctctc
    14461 ggcctcgtcg gcggcggcac gcagggcgtc catagcggga atgacctcgt cgtggaaggc
    14521 gtgtgccttg gcggcctcgt cgtccatggc gctgaccttg tcggtgactt cggtcagggt
    14581 gtcggcagcg tcggacattt cgtcggtgta cttgctcagg cgggtcagga ctttcgtctc
    14641 ggccttggtg gagataccct cgactgcggc ggtcttggcg gcggcgctgg atgcgacctc
    14701 ccccatgtaa gcggtggcag cggggatcag ctgcttgttg gcgatcttga gcatcgtgcg
    14761 ggcctcgatg ttgaggatct tggagtaatg ctccatctcg acctcgtagc ggctcagcat
    14821 ctcggtcttg gtcaggacgc cgaactcctc catcagggcg atgttcttgt cgtccttcag
    14881 ggcaatcata gcatcggggg tgcacttgcg gttcggcaaa ccgcggcgct cggcctcctg
    14941 ctcccacgca tcgctgtagc cgttgccgtt gaagatcacg cggcggtggg cgttcagtgt
    15001 tttcttgacc caggcggcgg cagcgcactc aaaatcggcg gcgtcggcgg tctcggccac
    15061 gaactccttc aggctcttgg ccacggcggt gttcaggatg gtgttcgcgt cggacaggtt
    15121 ctcggcagag ccgggcatac ggaactcaaa cttgttgccg gtgaaggcga agggggaggt
    15181 acggttgcgg tcggtggtgt ccttgctg
//
LOCUS       DBMDSQ010000018        14696 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_1281_length_14696_cov_2.185543, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000018 DBMDSQ010000000
VERSION     DBMDSQ010000018.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 14696)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 14696)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14696
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1281_length_14696_cov_2.185543"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            17..670
                     /locus_tag="ACZPAX_01390"
     CDS             17..670
                     /locus_tag="ACZPAX_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007045703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR1112583.1"
                     /translation="MLPILIVDDEPSIAQLIAMTLEPLGQPLIIAHDGERAADLLETQ
                     KFDLVILDVMLPGVDGFALMDYIAPTGTPVVFLTAKNAVADRVRGLKLGAYDYIVKPF
                     APPELLARVEGLLRHTGRRAATLNVFDAVIDPDDRTVTQHGQPVELTPREFDLLLALV
                     RAQGQALYRETLYERVWGLDAEPTPRALDLHIHRLRKKLNWANKIVTVPKIGYRLIK"
     gene            734..1984
                     /locus_tag="ACZPAX_01395"
     CDS             734..1984
                     /locus_tag="ACZPAX_01395"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR1112584.1"
                     /translation="MSRSIRLALVLFSLALTLTGSALACRTFRGQLDALETQAMLQHQ
                     RTVCGVESDFTAACAAARAVASTDNFTTPARIGDSYTRAQQLILGASTPAFALLRDGT
                     VEYSNTGLSGAALQQAAADTDTILLRDRQLLLASVLNTNQNIILVSAVDAAEAYTARD
                     QLLLSWLAVQALVTLAALAAGWHYGKVQELNAHQSRFVADLTHELKTPLTSMIGYADL
                     LRSGTLDDARRRTAADAIYHESTRLESLSQQLLALQGLQKNDIALQAVDVAAVFHDVA
                     RSLPNAPLTLDAPAGATVQADRVLLADLLRNLAVNALHASTPGNTVRLTCTDAGNGWR
                     LAVIDTGCGIPAEAVPHLTEPFYRVDKARARADGGSGIGLALCAEIAAAFGTKLNFAS
                     EVGAGTTVSLTLPKGGPRHEKAVS"
     gene            1965..2834
                     /locus_tag="ACZPAX_01400"
     CDS             1965..2834
                     /locus_tag="ACZPAX_01400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1112585.1"
                     /translation="MKKLSLNAKFITTSLFCLALLAVPALAVQGQALYYAAPQPRPQV
                     PGVMSDEVRLDPLAGALYRYGVQGGSSTPQSYDITRDVFTVLRGKLRQAAARSLLTDD
                     QLTYLLAQLDAGADTTPFQQSLTEGEPNVTYGMVKFTGDLTQVYFDYFIPRDVHETES
                     GGTACVMDTETYLVIDYLPDNGPIVDLSIHDENYPAGEDLDTVTQNFIAQLNTTIPGQ
                     WQQTTTPEDDPTYQRYIYAHDSGVLRVTSHHIHQQDFSLTVMLEPEVYGSLYPLNNLA
                     HDPYGQTYTAPLK"
     gene            complement(2956..3546)
                     /locus_tag="ACZPAX_01405"
     CDS             complement(2956..3546)
                     /locus_tag="ACZPAX_01405"
                     /EC_number="2.7.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007048003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0050145 - nucleoside monophosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HIR1112586.1"
                     /translation="MKRIITIGREFGAGGGELGRRLAREMNIAYYDRDIILKTAKASA
                     HLTPEQVRRWDERVPHEFGFTQSLFNFYNRPLSEELWDAQVRAIREIADKESCVIVGR
                     NADYILREFDHCLRVFVHADRNWRLLHMRKLMPDTPFDQLEADMDSADRARRNYCKQH
                     TGQVYGDSRNYDLTLCTSKLGLDKALEVLLCAAKEL"
     gene            complement(3691..3996)
                     /locus_tag="ACZPAX_01410"
     CDS             complement(3691..3996)
                     /locus_tag="ACZPAX_01410"
                     /inference="COORDINATES: protein motif:HMM:NF012698.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HIR1112587.1"
                     /translation="MKTAQDVFYKDLEVNLAAHRVSREGHELCLTPREFALLAVLLTH
                     CDKALSRSQLLEAAWGYAYIGESRTVDVHINRLRRKLGLENDIQTVYKVGYRLNSAS"
     gene            4402..6294
                     /gene="ftsH"
                     /locus_tag="ACZPAX_01415"
     CDS             4402..6294
                     /gene="ftsH"
                     /locus_tag="ACZPAX_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007048408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HIR1112588.1"
                     /translation="MKETTQPPKDPKKKLWIGYAVILSIVILSNIFLFPALMQASVKS
                     VNYSTFLQMLEDKELSTVQIEDQQIYFVDKNENSYKTNAIAQDNNLVARLEDAGVEFG
                     TVYQSPTIWDSLLNLVISFLPMILLFWFVNRWASKKMQEMGGAGGNAMLFGGGKSGAK
                     QYVVDDETGIKFNDVAGEDEAKESLQEIVDFLHNPKKYEDIGAKMPKGVLLVGPPGTG
                     KTLLARAVAGEAGVPFFSIAGSEFVEMFVGMGASKVRDLFKQAGEKAPCIVFIDEIDT
                     IGKKRDGGANLGGNDEREQTLNQLLTEMDGFDAAKGVVILAATNRPESLDPALTRPGR
                     FDRRVPVELPDLKGRESILRLHAKKVKLGPDCDFGVVARMTPGASGAELANIVNEAAL
                     CAVRHRRKAVTQFDLQEAVDTILAGAQKKNKILNNKEKCIVSYHEVGHALVAALQTNS
                     APVQKITIVPRTSGALGFTMQVEDGDHTLMTKEEILNKIATLTGGRAAEELIFHSITT
                     GASNDIEQATKLARALVTRYGMTEDFDMVALETVNNAYLGGDASLACSEQTAAKVDAK
                     VVEIVQAEHRKAYQLLSDNKRKLDEIAQYLYEKETISGEEFMRILNAQPQLPAAPLAN
                     STKAAE"
     gene            6390..6629
                     /locus_tag="ACZPAX_01420"
     CDS             6390..6629
                     /locus_tag="ACZPAX_01420"
                     /inference="COORDINATES: protein motif:HMM:NF012759.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HIR1112589.1"
                     /translation="MTFEELQDLLAEQFSCDTGELGRGVTLADLGADHEDMVELAWAL
                     GEAIGEEIDESELSDEATIGELWRFIRDKAENAEL"
     gene            6842..8389
                     /locus_tag="ACZPAX_01425"
     CDS             6842..8389
                     /locus_tag="ACZPAX_01425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1112590.1"
                     /translation="MTNTKSKHLLAVAALLAAALAMMLFYTTQKQGYHVDELYTYELT
                     NYPGSFYALSDGYMDTWHDGSFYAGVLSAGSPADYRIPWNNQKADVHPPLYYCLIYTA
                     ELIFPHLGLPWVGLLPNYVCLLAGGVLLYLAARRLTGRFWPAWCAAACWLLSVGVQGM
                     AVFTRMYSLMMLEGIALLYCHVVLWQALQQGRKPSRAVWLGLFAATLAGALTQYFFLV
                     FCFFLCGLFGLWLLVTRRFKTAGRYAAAEFAALGAAFLAFPTMKTHIFSGSRGKAAFS
                     SVFDLSALADWAASLGRVFRLLAAQFGGLLLWAVVLGAAALVLWRRGLRLRGSGLFAA
                     GLLLASCGYIILIDKAAPFEADRYYVIVYAAVVLAVSVVLAKLAANKDALLALALVPI
                     LAAHFLDPNGYLFEQYAPRTPALAETASLPAVVLNKAGYEVAPDLFITEFAQREAVYQ
                     AGGRDDAASLQNAAATHDLSGGFVLYGYIYNADDLLTLAESTLPIAHAELLTDTARCP
                     VYYIKLK"
     gene            8409..10184
                     /locus_tag="ACZPAX_01430"
     CDS             8409..10184
                     /locus_tag="ACZPAX_01430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1112591.1"
                     /translation="MPSRKKLSPLALVALLFFACTIVYGLLSSYPRQLAVYSDELRYL
                     DVARSLLAGHGLRVRNMPSDYQKILYPLCILPALLLKSTAAQITAIGWLNAAYMASAV
                     FPAYALAKTMRMNPHRTVFLVGVTAVLPTMSAASTFMSETVFLPLSLWQVYFFLRAML
                     AAPRARIGWCAAAGAFCYLLYLNKEVALYYLIAWVLVRTWVWWHDKASWKAELAATAA
                     LLGSFLACFLLAKATLFRGLGNSYNQTSWLNAEQWRFLPFALACDALFTVLAFWVFPV
                     LLPICGLHRPRKGSDARRTQLPLFLLLSLAIGVAVIAWSITVREDLHSPSPRQHMRYL
                     EPLLIPLLAVVLDTLDEPLTQMRRRVLAALTALWGVGFVLVCRAIGAGAGDNTLLQWF
                     DFIADRLDRLPALGLNGWLAVWRVVIAAGAAVLGVLLVRRKYRRPLAVAALALCVLCY
                     AGEWRINRWTYAIPAETAARASALNDALQSLDGTVLFLPNGIRQRDSQLIETYISRDF
                     YIVEYETLQASGQLADGVLDLTAEAVGPEYPGHPYTDLTAADWLLVADGVPVDTAALE
                     HSGIPCPDGYTLYRNPTPGLVAFLV"
     gene            10269..10787
                     /locus_tag="ACZPAX_01435"
     CDS             10269..10787
                     /locus_tag="ACZPAX_01435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1112592.1"
                     /translation="MGLINTIQTKTEQARAKERIRPLLESRMMRDILLEISQRGTEKL
                     DPQSVPLDQRPLASKRAAECGTAGLQQSVIREILLDETGIVLYAGSDEVKFLYGDYNY
                     ENIDDTDCLPAVAQYLVQHLLGYYNITRTFYSVSKHNGRSVAEKTRQVFIKRHHEDTP
                     FDPQPTRKQKLF"
     gene            complement(10883..13156)
                     /locus_tag="ACZPAX_01440"
     CDS             complement(10883..13156)
                     /locus_tag="ACZPAX_01440"
                     /inference="COORDINATES: protein motif:HMM:NF018884.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HIR1112593.1"
                     /translation="MAVLSGERLHTNMDKLLPGARSAALLWQGAALAGGLALGAGQVY
                     GGAAPFGLALVISCPPAYCLAAAVGTLAAGIAFQPALLGIKLGAAAVAAATVRRLIDE
                     RPKAGLLAGCLTLAAAQMVQIILLGGLVNFSQTVTVGCTALLAAGLGCAFAHFPAREP
                     RGVCLWLAAVTACLQRCAVGPLAPGLALAAGAGLCAAIGGTLEQTAVLSIALAAAITA
                     SGPTLAFAALAVAMGSLAAACLCPGERWRCAGVFTAGCTVGALAAPDAAGALPLAVSA
                     GVGIAAAMAVPGGVMRKIFPPPAPPVQAQGLSGAARKLASVADTLSDIADTVNAVCQR
                     QMPPKGESFDFVVEQVARTTCQSCTRRNRCWVRGYATAMDGLYHLKPILEGQGRVEVQ
                     DLPGQLSVCIHPADLCTAANHGYRLWRSRRQTRARASMLRTALTEQYSALAGALAQLA
                     GKLGQAGLPDPRREAKVAQLFADLGLDALECSVTADLAGRLTASVTICRTHFTQDEVR
                     GLTDEVSRICRRDMDTPEITHCRTVTMLTFGERPLFTVEFGAAAHAASGQPVSGDALD
                     QFCDTGGRAQMLLCDGMGTGRAAAVDGQMAAKLTAQLLRAGFAAESAARLVNVALGLK
                     GAEQEAGATLDLLTVDLYTGRAGLFKAGAAPSFLVRGGVPRMLDGASLPMGVLDSLVG
                     RSTTFALDAGDWVVLVSDGALTDGSDWLMQQLQLCAKLGHTPKQAAETVADAAACRAG
                     EKRDDITVAVLAMGKRA"
     gene            complement(13521..>14696)
                     /locus_tag="ACZPAX_01445"
     CDS             complement(13521..>14696)
                     /locus_tag="ACZPAX_01445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043163 - cell envelope organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HIR1112594.1"
                     /translation="YYVLSVVGILAKRMLFSALATAYVRRHDIVPRTLIIGSGELAHR
                     FYNEVFREKKTMAMRYSGYLAAAPCADLPDYCGTVADLFEVVRTQKIEMLVLAQDVQD
                     AEEVRRIMLLADNFHLRVAAVPVYNDFVTARSELDSIGGMRYMDLRLMNTCDIMGVNI
                     VVTDMDKTLRLIEEKLEDWRGKYICVANVHTTVTAHDDPDYQKVQNGAVMALPDGGPL
                     SKYSRQQGYVDAARVTGPDLMKELLKESKTKHYRHYFYGSTQKTLDVLREKVTENYPG
                     AVIAGMYSPPFRPLTPEEDEDVVRMINEAKPDFVWVGLGAPKQERWMAVHEDRVQALM
                     VGVGAAFDYEAGNIRRAPQWMQKHNLEWLYRLMQDPKRLFKRYFVTNTKYLWWTWRQ"
BASE COUNT         2722 a         5017 c         4188 g         2769 t
ORIGIN      
        1 gaaggaggta ttccccatgc tgccaattct gatcgtcgac gacgagccga gcattgccca
       61 gctgattgcc atgacgctgg aaccgctggg ccagccgctc atcattgcgc acgacggtga
      121 acgcgccgcc gatctgctgg aaacgcaaaa atttgacctg gtcatcctcg atgtcatgct
      181 gccgggcgtc gacggttttg cgctgatgga ttacatcgcg cccaccggca cgccggtcgt
      241 atttttaaca gccaaaaacg ccgtggccga ccgggtgcgt gggctgaaac tgggcgcata
      301 tgactacatc gtcaagccct ttgctccgcc ggaactgctg gcccgggtgg aggggctttt
      361 gcgccatacc ggccgccgcg cggccacgct gaacgtgttc gacgcggtca tcgaccccga
      421 cgaccgcacc gtgacccagc acggccagcc ggtggagctg accccgcggg agtttgacct
      481 tctgctggcc ctcgtccgcg cccaggggca ggcgctttac cgcgagacgc tttacgagcg
      541 ggtctggggc cttgacgccg agccgacgcc ccgcgccctc gacctgcaca tccaccgcct
      601 gcgcaaaaaa ctaaactggg ccaataagat cgtcaccgtg cccaaaatcg ggtatcgctt
      661 aataaaatga cgtttctccg taggggccgg gcatacccgg cccctacatg cccaccccga
      721 agggaggttc cccatgtccc gctccatccg tctcgccctt gtccttttct cccttgctct
      781 caccctcacg ggcagtgcgc tggcctgccg caccttccgc ggccagctcg acgcactgga
      841 aacccaggcc atgctccaac accagcgcac cgtctgcggc gtggaaagcg acttcaccgc
      901 cgcctgcgcc gctgcccgcg cggtcgcctc taccgacaac ttcaccaccc ctgcccgcat
      961 cggcgacagc tacactcgtg ctcagcagct catcctcggc gccagcacgc ccgcctttgc
     1021 acttctgcgt gacggcacag tcgaatactc caacaccggc ctgtccggcg ccgcgctcca
     1081 acaggccgct gcggacaccg acacgatcct gctgcgcgac cgccagctgc tgttggccag
     1141 cgtgctgaac acgaaccaga acatcattct tgtctctgct gtcgacgccg ccgaagccta
     1201 cacagcccgc gaccagctgc tcctcagctg gctggccgtg caggcgcttg tgacgctggc
     1261 cgccctggca gcgggctggc attacggcaa agtgcaagag ctgaacgccc accaaagccg
     1321 ctttgtggct gacctgaccc acgagctgaa aaccccgctg accagcatga ttggctatgc
     1381 ggaccttttg cgcagcggca cgttagatga cgcccgccga cgcacagccg ccgacgccat
     1441 ctaccacgag tccacccggc tcgagagcct gagccagcag ctattggccc tgcaagggct
     1501 gcaaaagaac gatatagcgc tgcaagccgt tgacgttgcc gccgtgttcc acgatgttgc
     1561 ccgcagcctg cccaatgcac cgctcaccct tgatgccccc gccggggcca cggtacaggc
     1621 cgaccgggtg ctgttggcgg acctgttgcg caacctggcc gtgaacgccc tgcacgccag
     1681 cacccccggc aataccgtgc gcctgacctg caccgacgcc gggaacggct ggcggctggc
     1741 cgtcattgac accggctgcg gcatccctgc cgaggccgtc ccccacctga cggagccgtt
     1801 ttaccgcgtt gacaaagccc gcgcccgggc cgacggcggc agcggcatcg gcctggccct
     1861 ctgcgccgaa attgccgcag cctttggcac gaagctcaac tttgccagcg aggtcggcgc
     1921 aggcacgacc gtctccctca ccctgccgaa aggaggcccc cgccatgaaa aagctgtctc
     1981 ttaacgcaaa attcatcacc acatcgctgt tctgtctggc actgctggcg gtgcccgcgc
     2041 tggccgtgca ggggcaggcg ctttactacg ccgcgcccca gccccgcccg caggtgcccg
     2101 gcgtcatgag cgacgaggtt cggctcgacc cgctggccgg ggcactctac cgctacggcg
     2161 tgcagggcgg ctccagcact ccgcagagct acgatatcac cagggacgtg ttcacggtcc
     2221 tccgcggcaa gctgcggcaa gccgctgccc gcagtctgct gaccgacgac cagcttacct
     2281 atctgctggc ccagctggac gcgggcgcgg acaccacccc tttccagcaa agccttaccg
     2341 agggcgaacc caacgtcacc tacggcatgg taaagtttac cggtgacctg acacaggtct
     2401 actttgacta ctttatcccc agggatgtac acgaaaccga gagcggcggc accgcctgcg
     2461 tcatggacac cgagacttac ctcgtcatcg actacctgcc cgacaacggc ccgattgtgg
     2521 acctgtccat ccacgacgaa aactaccccg ccggggagga tctggacacc gtgacgcaga
     2581 atttcattgc ccagctgaac accacgattc ccggccagtg gcagcagacc acgacccccg
     2641 aggacgaccc gacttaccag cgctacattt acgcccatga ttccggcgtg ctgcgggtga
     2701 cgtcgcacca catccaccag caggatttca gcctgaccgt catgctggag cctgaggtct
     2761 acggcagcct gtaccccctg aacaacctgg cccacgaccc ctacggccag acctacaccg
     2821 cacccctaaa ataaaaagca caaaaaacgc cctgcaccgc aaaaatacgg tgcagggtta
     2881 tttttatctt tcagtatctt gtaggggccg ggcattcccg gcccgcacgc ttatgggggc
     2941 tgcccctttc tccctttaca gttccttcgc cgcacacagc agcacttcca gggccttatc
     3001 cagccctagc ttgctggtgc agagcgtcag atcatagttg cggctgtcgc catagacctg
     3061 gccggtatgc tgcttgcagt agttgcggcg ggcgcggtca gcgctgtcca tatccgcttc
     3121 cagctggtca aacggcgtgt ccggcatcag cttacgcata tgcagcagac gccagttgcg
     3181 gtcagcgtgt acaaacaccc gcaggcagtg gtcaaactcc cgcaggatat agtctgcatt
     3241 gcggccgacg ataacacagc tctccttatc cgcaatttcc cggatggccc gcacctgggc
     3301 gtcccacagt tcctcgctca aggggcggtt gtagaagttg aacaggctct gggtaaagcc
     3361 gaactcgtgg ggtacgcgct cgtcccagcg gcgcacctgc tcgggtgtca ggtgggcgct
     3421 ggccttggcg gtctttaaaa tgatatcgcg gtcatagtag gcaatattca tctcccgcgc
     3481 caggcgtcgg cccagctcgc cgccgcccgc accaaactcg cgtccgatgg taatgatacg
     3541 cttcatggtc gtttcctcct tacagcgttt acagcggggc cattgcaggg gcggatgcaa
     3601 gcatcgccct ctgcaagcct acggcaccac tgcaatttca ggatttctta ttactcctat
     3661 tgtaccaaat cccgcgggaa atgcaagggt tcagcttgca ctattcagcc gatatcccac
     3721 tttatatacg gtctggatgt cattttccag tcccagcttg cggcgcaggc ggttgatatg
     3781 tacatccacg gtgcggctct cgccgatgta agcgtacccc caggcggcct ccaacagctg
     3841 gctgcggctc agcgccttgt cgcagtgggt cagcagcaca gccagcagtg caaattcccg
     3901 gggcgtcagg cacagttcgt gcccctcgcg gctgacccgg tgcgccgcga ggttcacttc
     3961 caaatcttta taaaagacat cttgtgcggt tttcataacg aacatcctcc ttccgaacag
     4021 gacagctttt tgcgattcct cacatctatt ataacgacag atgtttccaa cttgttacaa
     4081 gattcgcgaa atttaaggca aattcaaaga ttctgtttaa attttagaag tttttatttc
     4141 tctgcgttcc cattttccac atttcaacac ttccctctca tcgtactatt ttgtaccttt
     4201 cccctgggtt ttccaccatt tcaacacagt tttccacatt tccacagtgg aatcctgtcg
     4261 aaaacctgtg caaaacgcgg aaaacccgtc ccagccccac cgttatgcgg attttttgcg
     4321 tttcgttcaa agttgcttca tacgctgttc attttttcag tccatgctat acttgtcaca
     4381 aaagaaaagg aggctacccc catgaaagag acgacacagc cgccgaaaga cccaaagaag
     4441 aaactttgga tcggctacgc ggtcattctt tccatcgtca ttttaagcaa tatcttcctc
     4501 tttcctgccc tgatgcaggc cagcgtcaag agcgtaaatt acagtacctt cctgcaaatg
     4561 ctggaggata aggagctttc caccgtccag atcgaggatc agcaaattta ctttgtggac
     4621 aaaaacgaaa actcctacaa gaccaacgcc atcgcgcagg ataacaacct tgtcgcccgg
     4681 ctggaggatg cgggtgttga gttcggtaca gtctatcaga gccccaccat ctgggattcg
     4741 ctgctgaacc tcgtcatttc cttcctgccg atgattttgc tgttctggtt cgtcaaccgc
     4801 tgggcatcca aaaagatgca ggagatgggc ggcgcaggcg gcaacgccat gttgttcggc
     4861 ggcggcaaat ccggcgcaaa acagtacgtc gtggacgacg agaccggcat caaattcaac
     4921 gatgtagcgg gcgaggacga agccaaggag agcctgcagg agatcgtaga cttcctgcac
     4981 aaccctaaaa aatacgagga tatcggtgcc aaaatgccca agggcgtgct gctcgtaggc
     5041 cctccgggca ccggcaaaac gctgttggcc cgcgccgtcg ccggcgaggc cggcgtgccg
     5101 ttcttctcca tcgcgggcag tgagttcgtc gagatgttcg tcggcatggg tgcgtcgaaa
     5161 gtccgcgacc tgttcaagca ggcaggcgaa aaagcgccct gcatcgtctt tatcgacgag
     5221 atcgatacca ttggcaaaaa gcgggacggc ggagcgaacc tcggcggcaa cgacgagcgc
     5281 gagcaaacgc tgaatcagct gctgaccgag atggacggct tcgacgccgc caagggcgtc
     5341 gtcatcctgg ccgccaccaa ccgcccggag tccctcgacc ctgcgctgac ccgccccggc
     5401 cgttttgacc gccgcgtgcc cgtcgagctg cccgatttaa agggccgcga gagcatcctg
     5461 cgcctgcacg cgaaaaaggt caagctcggc cctgactgcg atttcggcgt cgtcgcccgc
     5521 atgacccccg gtgcctccgg tgccgagctg gccaacatcg tgaacgaggc cgccctctgc
     5581 gctgtgcgcc accgccgcaa ggctgtgacc cagtttgact tgcaggaggc cgtggacacg
     5641 atcctggccg gtgcccagaa gaaaaataag atcctcaaca acaaagaaaa gtgcatcgtc
     5701 tcctaccacg aggtcggcca cgcccttgtg gccgccttgc agaccaacag cgccccggtg
     5761 cagaaaatca ccatcgtacc gcgcacgtcg ggtgcgctgg gcttcaccat gcaggtggag
     5821 gacggcgacc ataccctgat gacgaaagag gaaatcctca acaaaatcgc caccctgacc
     5881 ggcggccgcg cagccgagga actgattttc cactccatca ccaccggtgc atccaatgat
     5941 atcgagcagg ccaccaagct ggcccgcgcc ctcgtcaccc gctacggcat gaccgaggac
     6001 ttcgacatgg tcgccctgga gaccgtgaac aacgcctacc tcggcggcga cgcaagcctt
     6061 gcctgcagcg aacagaccgc agccaaagtc gatgccaaag tcgtggagat cgtgcaggcc
     6121 gagcaccgca aagcctacca gcttttgagc gacaacaagc gtaaactgga cgagattgcg
     6181 cagtatttgt acgagaaaga aacgatttcc ggcgaggagt tcatgcgcat tttgaacgca
     6241 cagccgcagt taccggcggc tcctttggca aattccacaa aagcagctga ataattttgt
     6301 gcagttgttg tccgtttgcc ccttgacgga actgccgtgg gcttgtatga taagaacaca
     6361 aaacatccca gtgagagggg gaaacaagcg tgacctttga agaattgcag gacctgctgg
     6421 ccgagcagtt cagctgtgat accggtgagc tgggccgcgg cgtgacgctg gccgacctgg
     6481 gcgctgacca cgaggacatg gtagagcttg cctgggcgct gggcgaggcc atcggcgaag
     6541 agatcgacga aagcgaactg agcgacgaag ccaccatcgg tgagctgtgg cgcttcatcc
     6601 gcgacaaagc agaaaacgca gaactgtaaa ccctaaaaaa ggcctgcccc accggcaggc
     6661 ttttttggta tgctatccta taaaatatcc ctatcaaaac ccggcctttt gccttatatc
     6721 gaatgcaggg gcgaacattg ttcgcccgcc agcttgaccc ggcaatgctt ttccgggaaa
     6781 gcctcgggcg ggcaatgctc gctcctacac aaacccaccc atacaagaaa gaccaccatc
     6841 catgaccaac accaaatcca aacatctcct tgcggtcgcg gcactgctgg ccgccgccct
     6901 ggcaatgatg ctgttctaca ccacccaaaa acagggctac catgttgacg aactctacac
     6961 ctacgagctg acgaactatc ccggcagttt ctatgcgttg agcgatggct atatggacac
     7021 ctggcacgac ggcagttttt acgccggggt tctctctgcg ggcagccccg cagattaccg
     7081 catcccctgg aacaaccaaa aagccgacgt ccacccgcca ctctactact gcttgatcta
     7141 cacagccgag ctgattttcc cgcatctggg gctgccatgg gtggggcttc tgcctaacta
     7201 tgtctgcctg ctagcgggcg gcgtgctgct gtacctggcg gctcgtcggc tgactgggcg
     7261 gttctggccc gcctggtgcg cggcagcttg ctggctgctc agcgtgggcg tgcagggcat
     7321 ggcggtcttt acccggatgt acagcctgat gatgctggaa ggcatcgcac ttctctattg
     7381 ccatgtcgtg ctgtggcagg ctttgcagca gggccgcaaa ccgtcccgcg ccgtctggct
     7441 ggggcttttt gccgccacgc tggccggggc gctgacccag tatttcttcc tggtgttctg
     7501 ctttttcctc tgcggactgt tcggcctttg gctgcttgtc acgcggcggt tcaaaacagc
     7561 cgggcgctat gcggcggccg agtttgccgc gctgggggcc gcgttcctgg cctttcccac
     7621 catgaaaacc catatcttct ccggctcccg cggtaaggcg gcgttttcca gcgtgttcga
     7681 cctttccgct ctggccgatt gggccgccag cctgggccgg gtgtttcgcc tgctggccgc
     7741 ccagttcggc gggctgctgc tttgggccgt tgtgctgggt gcggccgccc tggtgctgtg
     7801 gcggcgtggg ctgcgcctgc gcggtagcgg cctgtttgcc gctgggctgc tgctggcatc
     7861 ctgcggctac atcattctta ttgacaaggc cgcgccgttt gaggctgacc gctactatgt
     7921 catcgtctac gctgccgtcg tgctggctgt ctccgtcgtg ctggcaaagc tggccgccaa
     7981 caaagacgca ctgctggcgc tggcgcttgt cccgattctg gccgcgcatt tcctcgaccc
     8041 caacggctat ttgtttgagc agtacgcccc gcgcacccca gccctggccg agaccgctag
     8101 cctgcccgcc gtggtgttga acaaagccgg gtacgaggtc gcgcccgacc tctttattac
     8161 cgagtttgcc cagcgggagg ccgtctatca ggccggcggc cgggatgacg cggccagcct
     8221 gcaaaacgcc gctgccaccc acgacctgag cggtggcttt gtgctgtacg gctacattta
     8281 taatgccgac gacctgctga ccctggctga atccactctc cccattgccc acgccgagct
     8341 attgaccgac accgcccgat gcccggtgta ttatatcaag ttaaaataat tcactaaagg
     8401 atttttccat gccatcacgt aaaaaactct cccccctcgc cctggtagcg ctgctgttct
     8461 ttgcctgcac catcgtgtac ggcctgctca gcagctaccc gcggcagctg gccgtgtaca
     8521 gcgacgagct gcgctatctg gatgtagccc gcagcctgct ggccgggcat gggctgcgcg
     8581 tgcgcaatat gcccagcgac taccagaaaa ttctctatcc gctctgcatc ctgcctgcgc
     8641 tgctgctcaa atccaccgcc gcgcagatca cggccatcgg ctggctgaac gccgcctaca
     8701 tggccagcgc ggttttcccg gcctatgcgc tggcaaaaac catgcggatg aacccccacc
     8761 gcacggtgtt cctggtgggc gtcacggcgg tgcttcccac gatgtccgcg gcctccacct
     8821 tcatgagcga gacggtcttt ctgccgctct cgctgtggca ggtgtatttc ttcctgcggg
     8881 ccatgctggc tgcgccccgg gcgcgcattg gctggtgcgc ggcagcgggc gcattctgct
     8941 acctgctcta cttaaacaag gaagtggcac tctactacct gatcgcctgg gtgctcgtcc
     9001 gcacctgggt ctggtggcac gacaaagcca gctggaaagc tgagcttgcc gccaccgccg
     9061 cgctgctggg cagttttctc gcctgctttt tgctggcgaa ggccacgttg ttccgggggc
     9121 tgggcaactc ctacaaccag acaagctggc tgaacgccga gcagtggcgt tttctgccct
     9181 ttgccctcgc ctgcgacgcg ctgttcacgg tgctggcgtt ctgggtgttc ccggtgctgc
     9241 tgccgatctg cgggctgcac cgcccccgca agggcagcga cgcccgccgt acccagctgc
     9301 cgctgtttct gttgctgtct cttgccatcg gtgtggcggt catcgcctgg tccatcaccg
     9361 tgcgggagga cctgcactcc ccctcgcccc ggcagcatat gcggtacctg gagccgctgc
     9421 tcatcccgct gctggccgtg gtgctggaca cactggacga acccctgacc caaatgcgcc
     9481 gccgcgtgct ggccgcgctg accgccctct ggggcgtcgg cttcgtgctg gtctgccggg
     9541 ccatcggtgc gggcgcgggg gacaacaccc ttttgcagtg gtttgacttc atcgccgatc
     9601 gtctcgaccg cctgcccgcc ctggggctga acggctggct ggccgtctgg cgcgtcgtca
     9661 ttgctgcagg cgcggccgtg ctgggcgtgc tgctggtgcg ccgcaaatac cggcgcccgc
     9721 ttgccgttgc cgcgttggcg ctctgcgtgc tgtgctatgc gggcgagtgg cgcatcaacc
     9781 gctggaccta cgccatcccc gccgaaactg ccgcccgggc ctcagccctg aacgacgcgc
     9841 tgcaatcgct ggacggcact gtgctgtttc tccccaacgg catccgccag cgggacagtc
     9901 agctcatcga aacctacatt tcccgtgatt tctacattgt ggaatacgag acgctgcaag
     9961 cctccggcca gctggcagac ggcgtgctgg acctgactgc cgaggccgtc ggtcccgagt
    10021 acccgggcca cccctacacc gacctgaccg ccgccgactg gctgctggta gccgatggcg
    10081 tacctgtgga caccgccgcg ctggagcaca gcggcatccc ctgcccggac ggctataccc
    10141 tctaccgcaa ccccacgccg gggctggtcg ctttcctcgt gtaattttta caatccagcc
    10201 tgttgccatt ggggcgcgaa gcatggtata ctgtatgtgt atcgtccatt ttcgcaagga
    10261 ggcgccctat gggcctgatc aacaccatcc agacaaaaac cgaacaagcc cgcgcgaagg
    10321 agcgcatccg cccgctgctg gaaagccgca tgatgcgcga tattctgctt gagatcagcc
    10381 agcgcggcac tgaaaagctc gacccgcaga gcgtcccgct ggatcagcgc cccttggcct
    10441 ccaaacgcgc cgccgagtgc ggcaccgccg ggttgcagca gagcgttatc cgggaaatat
    10501 tgctcgacga gaccggcatc gtgctctatg ctggcagcga cgaggtcaag ttcctctacg
    10561 gcgactacaa ttacgagaac atcgacgaca ccgactgcct ccccgccgtg gcgcagtatc
    10621 tggtgcagca cctgctgggg tattacaaca tcacccggac gttctactcg gtgtccaagc
    10681 acaacggccg cagcgtagcc gaaaagaccc gccaggtctt tatcaaacgc caccacgagg
    10741 acaccccctt cgacccccag cccacccgga aacaaaaatt attttaaaac gcaccgcgcc
    10801 ccatgccagc aacgcatgga gcgcggtttt gttatacgtc tttccccatt gtctgttcct
    10861 gcgggcgggg ccacgcccgc ccctacgccc tcttccccat cgccagcacc gccacggtaa
    10921 tatcatcccg cttttccccg gcgcggcagg cggcggcatc cgctaccgtc tcggcggcct
    10981 gcttgggggt gtggcccagc ttggcgcaga gctgtaactg ctgcatcagc caatcgctgc
    11041 cgtctgtcag ggcaccgtcg ctgaccagca ccacccagtc ccccgcatcc agcgcaaagg
    11101 tcgtggagcg gcctaccaaa ctgtccagca cgcccatggg caggctggca ccgtccagca
    11161 tccggggcac gccgccccgc accagaaagc tcggcgcagc cccggccttg aacagcccgg
    11221 cccgcccggt gtacaggtcc accgtcagca ggtccagcgt cgccccggcc tcctgctcgg
    11281 cccctttcag ccccagcgcc acattcacaa gccgcgcggc gctctcagcg gcaaagcccg
    11341 cccgcagcag ctgggctgtc agtttggcgg ccatctggcc gtccacggcg gcggcgcggc
    11401 ctgtgcccat gccgtcgcac aaaagcatct gcgcccggcc gccggtatcg cagaactggt
    11461 ccagcgcgtc gccgctgacc ggctggccgc tggccgcgtg ggccgccgcg ccgaattcca
    11521 ccgtgaacaa gggccgctcc ccaaaggtca gcatcgtgac ggtgcggcag tgggtgatct
    11581 ccggcgtgtc catatcgcgg cggcagatgc ggctcacctc gtcggtcagg ccgcgcacct
    11641 cgtcctgggt aaagtgagtg cggcagatcg ttacgctggc ggtcagccgc ccggccaggt
    11701 cggcggtcac gctgcattcc agcgcgtcca gccccaggtc cgcaaacagt tgggccactt
    11761 ttgcctcccg ccgggggtcc ggcagcccgg cctggcccag tttgcccgcc agctgggcca
    11821 gcgccccggc cagggcgctg tactgctcgg tcagggcggt gcgcagcatc gacgcccgcg
    11881 cccgggtctg gcggcggctg cgccacagcc ggtagccgtg gttggccgcc gtacacagat
    11941 ctgccgggtg gatgcagact gacagctgcc ccggcaggtc ctgcacctca acgcggccct
    12001 ggccctccag aatcggcttt agatggtaca gcccatccat ggccgtggcg tagccccgca
    12061 cccagcagcg attacgccgg gtgcagctct ggcaggtggt gcgggccacc tgctccacca
    12121 caaaatcaaa gctctcgccc ttgggcggca tctgccgctg gcagactgcg ttgaccgtgt
    12181 cggcaatatc acttaaggtg tcggccacgc tggccagctt gcgggccgcg ccgctcaacc
    12241 cctgggcctg caccggcggt gcgggcggcg gaaaaatttt gcgcatgacc ccgcccggca
    12301 cagccatggc cgccgctatg cccaccccgg cactgacggc cagcggcagc gcaccggcgg
    12361 catccggtgc ggccaatgcc cccaccgtgc agcccgccgt gaacaccccg gcacagcgcc
    12421 aacgctcgcc cgggcagagg caggccgcgg ccaggctgcc cattgccacg gccagcgccg
    12481 caaaagccag tgtcggccct gatgccgtga tggccgccgc caacgcaatg cttaaaaccg
    12541 ccgtttgttc cagcgtgccg ccgatggctg cgcacagccc cgccccggcc gccagcgcca
    12601 ggcccggagc caaaggcccg acggcacagc gctgtaagca ggccgtgacc gctgccagcc
    12661 acaggcagac gccccgcggc tcccgcgcgg gaaaatgcgc gaacgcacag cccagccccg
    12721 ccgcaagcag ggccgtgcac cccaccgtga ccgtctggga aaaatttaca aggcccccca
    12781 gcagaatgat ttgcaccatc tgcgccgccg ccagggtcag gcagcccgcc agcagcccgg
    12841 ccttggggcg ctcatcgata agccgccgca ccgtggccgc agctaccgcc gccgcgccca
    12901 gtttgatgcc cagcaaggcg ggctggaacg ctatccccgc cgccagcgtg cccaccgctg
    12961 ccgccaggca gtaggccggc gggcagctga tgaccagcgc cagcccaaag ggggccgccc
    13021 cgccgtagac ctgcccggcc cccagcgcca gcccacccgc cagcgccgcg ccctgccaca
    13081 gcagcgccgc gctgcgcgca ccgggcagca gcttgtccat attggtatgt aagcgttccc
    13141 cactcaaaac agccatcgtt gtttccctcc gcaaatatac agtgtatgtc cggacgcttt
    13201 gcactatacc acgccccgcc gccgcatttt gccgcagagc gcagaaaccc gaaaacaacg
    13261 tataaacaaa caaaaaagag caccgcagcc cataaaagac tgcgatgctc ttgtgtaaag
    13321 gcttccccct tgggggaagc tgtcgccaaa ggcgactgat gaggggcagg tttgccgatg
    13381 caacccgtct accgggcagt cgcggcaaac ttgcccctca tccggccctt cgggccacct
    13441 tcagtctacg cgctgagagc cttcttcggc ggttgcgctc cgacacgcgc ctgcgggcgc
    13501 agtccccggg agaaggcttt tcactgccgc cacgtccacc acaaatactt ggtgttcgtg
    13561 acaaaatacc gcttgaacag ccgcttgggg tcctgcatca ggcggtagag ccattccagg
    13621 ttgtgcttct gcatccactg cggggcacgg cggatattgc ccgcctcgta gtcgaaggcg
    13681 gcgcccacgc ccaccatcag cgcctgcacg cggtcctcat gtacggccat ccagcgctcc
    13741 tgtttcggcg cgcccaggcc cacccagaca aaatcgggct tggcctcatt gatcatccgc
    13801 accacgtcct cgtcctcctc gggcgtcagc gggcggaatg gcggggagta cattcccgca
    13861 atgacggctc cgggataatt ctccgtgact ttttcccgca gcacatccag cgttttctgc
    13921 gtggaaccgt agaaataatg gcggtaatgc ttggtcttgc tctccttcag caactccttc
    13981 atcaggtcgg ggccggtcac gcgggccgcg tccacatacc cctgctggcg gctgtatttg
    14041 gagagcgggc cgccgtccgg cagggccatg actgcgccgt tctgcacctt ctggtagtcg
    14101 gggtcatcgt gggctgtcac agtggtgtgg acatttgcca cgcagatgta tttcccgcgc
    14161 caatcttcca gtttttcctc gatgaggcgc agggtcttgt ccatatcggt cacgacgatg
    14221 ttcacgccca tgatgtcgca ggtgttcatc aaccgcaggt ccatgtaccg cataccgccg
    14281 atgctgtcca gttcgctgcg ggcggtcaca aagtcgttgt acaccggcac cgccgccacc
    14341 cgcaggtgga agttatcggc cagcagcatg atacggcgca cttcctcggc gtcctgcaca
    14401 tcctgggcca gcaccagcat ttcaattttc tgggtgcgca cgacctcaaa caggtcggcc
    14461 accgtgccgc agtagtcggg caggtcggcg cagggggccg ccgccaggta gccgctgtac
    14521 cgcatggcca tcgttttctt ttcccggaaa acttcgttgt agaaccggtg agccagctcg
    14581 ccgctgccga tgatcagcgt gcggggcaca atgtcatgcc gccgcacata ggcggtcgcc
    14641 agggcgctga acagcatccg cttggccagg atgcccacca ccgacaagac ataata
//
LOCUS       DBMDSQ010000019        14644 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_1287_length_14644_cov_2.014715, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000019 DBMDSQ010000000
VERSION     DBMDSQ010000019.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 14644)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 14644)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14644
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1287_length_14644_cov_2.014715"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..683)
                     /locus_tag="ACZPAX_01450"
     CDS             complement(<1..683)
                     /locus_tag="ACZPAX_01450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1112595.1"
                     /translation="MKKCAVILAPLIFLTACASAPAGSITPTPDPTATPVITPAPTEA
                     PTATPSPTMAVYEEVSFDDWRGDAGCRMPDYTFSAPHKPLLQQGEKVVWPTEGVTLRY
                     SADSLEEVAEIFHTTVENIKELNPDWQDNYSRNGGRYWDLKVQAEPYTVPENNVVTVT
                     VNAPWVEGYYDRTEVSDVPASLDKQARAALASAYYFQYHWFGMHAGFMPFESVEGLPG
                     WLQGYATDGA"
     gene            complement(813..2567)
                     /locus_tag="ACZPAX_01455"
     CDS             complement(813..2567)
                     /locus_tag="ACZPAX_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005921891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990112 - RQC complex [Evidence IEA];
                     GO_function: GO:0001968 - fibronectin binding [Evidence
                     IEA];
                     GO_process: GO:0072344 - rescue of stalled ribosome
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rqc2 family fibronectin-binding protein"
                     /protein_id="HIR1112596.1"
                     /translation="MSLDAATLALVAGELKTTLLDAKIDKIFEPTRDEVLMTLRTRTE
                     THRLLLSARSGSARVCLTKESFENPLTPPGFCMLLRKHLTGGRLIELRREPGDRIVYF
                     DFRCTNEMGDLVTNTLAVELMGRYSNLVLVQDGRIIDALKRVDFDDSEVRQLLPGLAY
                     TLPPKPARPDFLETSAASIVAAACEKDLPVAQALGKTVAGVGPVVVREAVCRALGETP
                     ALACDLTAEEKAKLAAAIDALKDEHAHGGTPTAVRLPQADGAPKPVEFSFFVPQQYGS
                     EAILTQYPSYSEMLEDYYATKDRAERLRQKSRELYKAVHNMYDRSVRKQAARKEELSQ
                     SAKADTLRLYGELLQANLWAIHKGDRQVTVQNYYTGEDVTIKLDPRLGGNENAQKYFR
                     DYKKKQTAHTMLQKLLVEGEAEIEYLRTVLYEVESAPGEMALNEIRTELKSQGYLKYY
                     KQRDRKQKPADFLRYTSSDGFEILVGRNNLQNDKLTLHTARGKDLWFHVQKAPGSHCV
                     VMSRGEDIPDTTRQEAAELAVLHSSQNGGAKVAVDTTEVKNIWKANGAKPGMVLYEVY
                     TTVYVTPRAGLEEQLKRK"
     gene            complement(2704..3459)
                     /locus_tag="ACZPAX_01460"
     CDS             complement(2704..3459)
                     /locus_tag="ACZPAX_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HIR1112597.1"
                     /translation="MSVSVQQILGLLQAVAPPALALDWDNVGLLVDAGTPVDGVLTTL
                     DITPAVVREAVENDCQLIVSHHPVIFHPIKSLHADDVPALLMKNGISAICMHTNLDAA
                     PGGVNDTLCDILGIAAEGRESFAEGCGRIGTTMPTTVEALAKFCADTLHSGVKYVNGQ
                     KPVTCLAEVSGGGGSYLQEAIDLGADCLVTGEAAHHIALLAKEKGVGLVVAGHWGTEH
                     AVADVLAAHIAKQFPQLTVAHAEADKDPYSYLL"
     gene            complement(3543..4217)
                     /locus_tag="ACZPAX_01465"
     CDS             complement(3543..4217)
                     /locus_tag="ACZPAX_01465"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005921895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR1112598.1"
                     /translation="MHTCKVPQLDARLAAAYAFVRAGRVAADIGCDHGKLSAALAGSG
                     RCPLVLACDLRPGPLEKARVTCAPYGDKVQFRLGSGLAVLAPGEAEDIIIAGMGAETI
                     IEILGAAPWVFDARYNLVLVPATKHSILRRWLARRGFALRGETLCQAAGRWYAVMNAQ
                     YTGDAREPDGLYCLTGLTQGQPGCADYYAVQNAKLKKYCLGLSDGAERDAVGELIAEM
                     DTLAAR"
     gene            complement(4204..4410)
                     /locus_tag="ACZPAX_01470"
     CDS             complement(4204..4410)
                     /locus_tag="ACZPAX_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HIR1112599.1"
                     /translation="MTITKNTIIAEILNNDPTQGCVPIFLATGMHCLGCMASHGETVE
                     EACAVHGVDPDELVAELNDYFAHL"
     gene            complement(4441..6690)
                     /locus_tag="ACZPAX_01475"
     CDS             complement(4441..6690)
                     /locus_tag="ACZPAX_01475"
                     /inference="COORDINATES: protein motif:HMM:NF021436.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2142 domain-containing protein"
                     /protein_id="HIR1112600.1"
                     /translation="MKKQLSLRRVVGSIAAAVLAAAAVWLLCRWVGYDLQLRVGNQPV
                     YEIVNDDYTQVIDLPEEGFDQAIPLQAGESLYGVRLRFSTHGQLYRAGMVMVDLCDAA
                     GNELRQAAGNYANVFDDNFTGFSFGAPYTAEQAETVYLHIYNAVEWEGPLGLWASETA
                     VKDLPLTADGKSQNATLALQYMTDYSGSWPTQLANELAPLLAGGAFAAVLLAGLQLPL
                     AVTVAVAGLLCGLAFVRVTPALVAPDEYTHLAAAYELASRLGGEQPADADGNLLVREC
                     DAPYFGTRTGEIGILAYKAEALAIEQEPGGPDELTAASTAKAGQGSGNYLPQALGLRL
                     ARSSGAGFYVMLRQARIFNLVFYLTLAVLAVALAPAGVRGLLACVALLPMPLQLAASL
                     SPDASVLGMVFCYMALCLRLRRKRAVWWEKILLIALGGAVGPAKAIYLPVVLLCFIIP
                     VENLMGSTEFVKGPFGARVRSGRLIQAAALILAGCLWISANLGELVYAARDISLAVLV
                     VVVAALAAAVGLVLWLYDKVHTDPKKLCLFRCGLGCAVVLAVVGGVFALSRMGGGLEP
                     DQLLMTNPNGDSIWTFSLGYICRNLPATTKLLLRTIPEQGALWLQGILGTTLGEPIVY
                     RIDVSWLLGVGLVLALLAAALPVQGTEAPLGRRTKIGVVGIILCVVASSLVVALNWTP
                     INYQTLFGLQGRYWLPILPLVLVLCKCGRNITLRHDTSRGAALAVTACTLLTLLQGFG
                     LYASWQPVS"
     gene            complement(6763..8181)
                     /locus_tag="ACZPAX_01480"
     CDS             complement(6763..8181)
                     /locus_tag="ACZPAX_01480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1112601.1"
                     /translation="MLTKQNARRAAWLVGIAAIALVLCSKSSPLYVLNDWMDANIFFT
                     MGKSMFRGKVLYRDVFDHKGPVLYFLYGLASLIDYTGFTGVLVVEIIAFALFLHCGLR
                     TAELLAGRALHPAWAVLPAAGIAASRAFAHGGSAEELLLPFLAFALYGAVKTLQDTHP
                     MPLRTVALHGFLAGCALWLKYTVLGFYLAWVVVLAVVYLWCGRAAQLGKSVAAYLGGM
                     ALATLPWVIYFGVNSALGDWFTCYFYDNLFLYKGEGGGALALAQHLWWAVRDALPAAV
                     LLTMFLIWAAAARKPSAVAAVAALAAGLALTSLMGGYLVYYGLVLAVFAPLGIVPLAT
                     LWGTRRNCGLLWLAAGAAWCFAFSPNHALRFRDADTMPQTRFAAKINGASLLNYGTLD
                     GGFYTTAGVLPPCKYFCVTNMPLDDQWSDQQAVLVNSAVGYVAALTGDLGGDFPQYKV
                     IDQCSYNGGEGEVTWYLYQLQR"
     gene            complement(8269..8547)
                     /locus_tag="ACZPAX_01485"
     CDS             complement(8269..8547)
                     /locus_tag="ACZPAX_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009064159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain containing protein"
                     /protein_id="HIR1112602.1"
                     /translation="MFEDKTLVCKDCGKEFVWTAGEQEFYASRGFENQPQRCKPCRDA
                     RKNATRGERQMFDATCASCGKACKVPFQPREDRPVYCSECFAKMKENG"
     gene            complement(8686..9252)
                     /locus_tag="ACZPAX_01490"
     CDS             complement(8686..9252)
                     /locus_tag="ACZPAX_01490"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR1112603.1"
                     /translation="MPLPKTTIEQDLPVRAQQGDENALAALIARMMPAIRSGAAACTA
                     PGLDFEDAVQEGLIGLFHAVRGFDAASGETFTAYAAASIRHAQQDARKAALRKKHAPL
                     NYSVPLPDAEESTDTRPGPEEIAISGEQYADTMRRMQTELSVLERRVIMAGLEGKTPA
                     QTAQELGLPAKNVANALARARRKLRGGP"
     gene            complement(9487..10506)
                     /gene="mreB"
                     /locus_tag="ACZPAX_01495"
     CDS             complement(9487..10506)
                     /gene="mreB"
                     /locus_tag="ACZPAX_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HIR1112604.1"
                     /translation="MFTKDIGIDLGTANTLVYMKGRGIIMREPSVVAVDPRSDELRVR
                     SVGHEAKAVIGRAPGSIVAVRPLKDGVIADFDITAAMLQSFIRQACGNSIFAHPRVVI
                     CVPSGVTEVERRAVRQAAAKAGARQVTVIEEPMAAAIGSGLPTTDAVGSMIVDIGGGT
                     AEVAVISLSGIVASRSVRCAGDALDQSIIAFIKRKYNLLVGERTAEQIKIEIGSACPQ
                     DPETSMEIKGRNLVDGLPKDILIRSEEVRDAMSECLLRIVEAIKDTLECTPPELSSDI
                     IDRGIMLSGGGALLRGLDTLIQNETGIDVHIAESPLDCVALGAGAVLDNPDLAGRRKE
                     EMSYL"
     gene            complement(10589..11143)
                     /locus_tag="ACZPAX_01500"
     CDS             complement(10589..11143)
                     /locus_tag="ACZPAX_01500"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009146 - purine nucleoside triphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HIR1112605.1"
                     /translation="MALILASGSPRRRELMSLITPDYTVITSDVDETKIAADSPAHLA
                     KALATAKARAVAEEHPDDIVCGFDTVVECEGEVFGKPKDEADAVRMLRALSGRAHRVH
                     TGVCICRGRRAAATVETTSVHFSSIEEDDLRIYVRTPEPYDKAGAYAIQGHAALWCAG
                     IEGCYYNIMGLPVHRTAQLLREFQ"
     gene            complement(11217..11660)
                     /gene="dut"
                     /locus_tag="ACZPAX_01505"
     CDS             complement(11217..11660)
                     /gene="dut"
                     /locus_tag="ACZPAX_01505"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015564727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HIR1112606.1"
                     /translation="MTPIVKYKKLDPRAVVPTYATPGAAAADLCAVLDEPLTLTPMQR
                     TLVPTGLAIELPGPECVALVYARSGLSIKHGLCMANGVGVIDSDYRGELRVPMVNLSN
                     EPYTIQPGERVAQLCIAPVWQAAFTSADELTDTQRGAGGFGSTGK"
     gene            complement(11810..13885)
                     /locus_tag="ACZPAX_01510"
     CDS             complement(11810..13885)
                     /locus_tag="ACZPAX_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HIR1112607.1"
                     /translation="MSRPEILAPAGNREMLGAAVFSGADAVYLGLTGFNARRTAGNFT
                     PDELREAVAFCHARGVKVHVTLNTLVYDRELAGLADAVRAVAEAGADAVIADDLATAQ
                     LVKSIAPTLHLHGSTQMSVHTPEGAKELAALGYDRVILARELSLDEIRAVCEASPIEV
                     EVFVHGALCMSVSGQCMMSAFLGGRSGNRGACAGPCRLPFDASAGLKPGQPGKACHLS
                     LKDMDYIPHLRELMDAGVASVKIEGRLRTPEYAAAVVTACRAVCAGQPYDEKLVRDIF
                     SRSGFTDGYLTNRNDGKMFGVRTEADAAATRAATPKARELFRRELQRVPVHYEVSGGV
                     EDGGIKLTAWDDDGHKAVVYSADEPQPAQKDPLPGIERALGKTGGTPFVMADLRTEAG
                     EGGLGFLPGSAWNELRREALDKLLEKRSKVRPHATQDITLPAYPAHTVGQAPVLAARF
                     ANAAQCPADAVEKLKYLIFPIAEAESIPEAWREKTLLELPRVMFGKLEQKTAARLDAL
                     QDAGFAGAVVNNPAQLRYTDGWTLYGGLGLNVTNPMSAARYKSLGIEGMLLQPETALT
                     AMQAVAPGVPTAALCYGHLPLMLTRACPLRNVRDCGKCQGGGTLRDRKGRDFTVTCSA
                     PGGAGVRTVYNPVPLYMGERLSEMPVDVAVAAFTIETPARVSQILALLLDARPFDNEF
                     TRGLYYTNN"
     gene            complement(13882..14253)
                     /locus_tag="ACZPAX_01515"
     CDS             complement(13882..14253)
                     /locus_tag="ACZPAX_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HIR1112608.1"
                     /translation="MATSKVRLNICGSSYVVNTSESEDYMQNLADRLNLDMNELMASS
                     NSVSITTAAVMTALNYRDELEKASGSADNMRRQIKDYLEDAASAKMAAEEARRENASL
                     KRRIDELERRLRRSQFPVEDE"
BASE COUNT         2764 a         4648 c         4491 g         2741 t
ORIGIN      
        1 gcaccgtccg tcgcatagcc ctgaagccat cccggcaaac cctccacaga ctcgaagggc
       61 ataaagcccg catgcatccc aaaccaatgg tactggaagt aatacgccga ggccagcgca
      121 gcccgggcct gcttatccag cgaagcagga acatcgctga cttctgtgcg gtcgtagtag
      181 ccttctaccc acggcgcatt gactgtgacg gtgacgacat tgttctcagg cactgtgtag
      241 ggctcagctt gcactttcaa gtcccagtag cgtccgccgt tgcggctata gttatcctgc
      301 caatccgggt tcagttcttt tatgttttcc accgttgtgt ggaaaatctc cgccacttct
      361 tccagcgaat ccgccgagta acggagcgtc acgccctcgg tgggccagac aactttttcg
      421 ccctgctgca gcagtggttt atgcggcgca ctgaatgtgt agtccggcat ccggcagccc
      481 gcatcgcccc gccagtcatc gaaagagact tcctcgtaca ccgccatcgt cggggacgga
      541 gtggctgtcg gcgcctcggt aggtgccggg gttatgacag gtgttgcggt cgggtcaggc
      601 gttggggtga tgctgcccgc cggggcagaa gcgcacgcag taagaaaaat aagaggtgca
      661 agaattacag cgcatttttt cataacgtac ctcatcgaaa gatgccctgt aggggccgca
      721 catgtgcggc ccgcagctgt atggcagacg actgcttacg ggaaagccga aggccgggcc
      781 tgcccggccc ctaccgtgcg ctgatggaat attcacttcc gtttcagctg ctcctccagc
      841 ccggcacgcg gggtcacgta gacggtggtg tagacctcgt acaggaccat gccgggcttg
      901 gcgccgttgg ctttccagat gttcttgacc tcggtggtat ccactgccac cttcgcgccg
      961 ccattctgac tggaatggag caccgccaac tcggcggcct cctgcctggt cgtgtcgggg
     1021 atatcctctc ccctgctcat gacaacgcag tggctgccgg gggctttctg cacatggaac
     1081 cacagatcct tgccgcgggc ggtgtgcagg gtcagcttgt cgttctgcaa gttgttccgg
     1141 cccaccagaa tttcaaaccc atcgctggac gtgtagcgca gaaaatccgc aggcttctgc
     1201 ttgcggtcgc gctgcttgta gtatttcagg tagccctggc tcttgagctc ggtccggatc
     1261 tcattcagtg ccatctcgcc cggggcactc tccacctcgt acaggaccgt gcgcaggtac
     1321 tcgatctcgg cctcgccctc caccagcagc ttttgtagca tggtgtgggc agtctgcttc
     1381 tttttgtaat cgcggaaata cttctgcgcg ttctcgttgc cgcccagacg cggatcgagt
     1441 ttgatggtca catcctcgcc ggtgtagtag ttctgcaccg tgacctggcg gtcgcccttg
     1501 tggatggccc agaggttggc ctgcaaaagc tcaccgtaca gccgcagcgt gtcggcctta
     1561 gcggattggc tcagttcctc tttgcgggct gcctgtttgc ggacggaacg atcgtacata
     1621 ttatggacgg ccttgtacag ctcgcggctc ttctggcgca ggcgctcggc gcggtccttg
     1681 gtggcgtagt aatcctcgag catttcgctg taactcgggt actgggtcag aatcgcctcg
     1741 ctgccgtact gctgcgggac aaagaagctg aactccaccg gtttgggcgc accgtccgcc
     1801 tggggcagcc gcaccgccgt cggcgtgccg ccgtgggcgt gctcgtcctt cagtgcatcg
     1861 atggccgccg ccagctttgc tttttcctcg gcggtcaaat cacaggctag cgccggtgtt
     1921 tcgcccagtg cgcggcagac agcctcccgc acgacgaccg ggccaacgcc cgccaccgtt
     1981 ttgcccaacg cctgggccac cggcagatcc ttctcgcagg cggccgccac aatagacgca
     2041 gcgcttgtct cgaggaagtc cggccgcgcg ggcttgggcg gcagcgtgta ggccaggccg
     2101 ggcagcagct ggcggacctc gctgtcgtca aagtccacac gcttcagcgc atcgatgata
     2161 cggccatcct gtacgagcac gaggttggag taacggccca tcagctccac ggccagcgtg
     2221 ttggtgacca ggtcgcccat ctcgttggtg cagcggaagt caaaatacac gatgcggtcg
     2281 cccggctcgc ggcgcagctc gatgagccgc ccgccggtca ggtgcttgcg cagcagcata
     2341 cagaaacccg gcggcgtgag cggattttcg aagctttcct tcgtcaggca aacgcgggcc
     2401 gaaccgctgc gggccgacag cagcaagcgg tgggtctcgg tgcgggtgcg cagcgtcatc
     2461 agcacctcgt cgcgggtcgg ctcaaaaatt ttgtcgatct tcgcgtcgag cagggtcgtt
     2521 ttcagttcac cggcgaccag cgccagcgtt gcggcatcta gtgacatagg gagaaacctc
     2581 cgagatacta taaggtaagc gagaagagcg aaaagagaag agatcctgac gcgagggaaa
     2641 agctgtatgc cgggcttttg ccacgccctg tctttctccc tcttctttct tctctttttt
     2701 ctactataat aagtaagaat acgggtcttt gtcggcctcg gcatgggcga cggtcagctg
     2761 gggaaactgc ttggcaatgt gggcggccag cacgtcggcg acggcgtgct cggtgcccca
     2821 gtggcccgcc acgaccaggc cgacgccctt ttcctttgcc agcagggcga tatggtgcgc
     2881 agcctcgccg gtgacaaggc agtccgcacc caggtcgatg gcctcctgta agtagctgcc
     2941 gcccccgccg ctgacctcag ccaggcaggt aacgggcttc tggccgttca catacttgac
     3001 gccgctgtgc agcgtgtcgg cgcagaactt tgccagcgct tccacagtgg ttggcatcgt
     3061 ggtaccgatg cggccgcagc cctcggcaaa gctttcacgc ccttcggcgg caatgccgag
     3121 gatatcacaa agggtatcgt tgacaccgcc cggtgcggca tccagattcg tgtgcataca
     3181 gatggcagag atgccatttt tcatcagcag cgcgggtaca tcgtcggcgt gcaggctttt
     3241 gatggggtgg aaaatgaccg ggtgatggct gacgatgagc tggcagtcgt tttccacagc
     3301 ctcccgcacg acggcggggg taatgtccag cgtggtcagc accccgtcca ccggcgtgcc
     3361 tgcatcgacg agcagtccga cgttatccca atccagcgcc agtgcgggcg gcgcgaccgc
     3421 ctgcagcagg cccaaaatct gctgtacact taccgacatg atgatttcct cctgtttcaa
     3481 gcattactgt gcaactgtcg tcgcagcatt gtagggggca aacattgttc gcccgcgggc
     3541 gtttaccgtg ctgccaacgt atccatttcc gcgataagct cccctaccgc gtcacgctct
     3601 gcgccgtccg acaagcccaa acagtacttt ttcagcttcg cgttctgcac ggcgtaatag
     3661 tccgcgcaac cgggctgccc ctgtgtcaga ccggtcaggc agtacagccc atccggttcg
     3721 cgggcatcgc ccgtgtactg cgcgttcatc acagcgtacc accgccccgc cgcctggcag
     3781 agcgtttcgc cccgcagggc aaaaccgcgc cgggctagcc accgccgcaa gatgctgtgc
     3841 ttggtggcgg gcaccagcac tagattgtac cgtgcatcaa acacccaggg agcggccccc
     3901 aatatttcta tgatcgtctc cgcgcccatg cctgcgatga tgatgtcctc ggcctcgccg
     3961 ggggccagca ctgccagccc gctgcccagg cggaactgca ccttgtcccc atagggggca
     4021 caggtcacgc gggctttttc cagcgggccg gggcgaaggt cacaagccaa aacaagcggg
     4081 cagcgaccgc tgcccgccag tgcggcgctt aatttgccgt ggtcacagcc gatatccgct
     4141 gccacgcgcc ccgcccgtac aaaggcatag gcggcggcca agcgggcatc cagctggggt
     4201 accttacagg tgtgcaaagt agtcgttcag ctcggcgacg agctcgtcgg gatcaacacc
     4261 gtggacagcg caggcttcct caacggtctc accgtgggag gccatgcagc ccaggcagtg
     4321 cataccggtg gccaggaaga tgggcacgca gccctgggtg gggtcgttgt tcaggatctc
     4381 ggcgatgatg gtattcttgg taatggtcat gagagtgttc tccttttaga atggtttgat
     4441 ttatgaaacc ggctgccagc tggcgtacag gccaaagcct tgcagcaggg tcaacagtgt
     4501 gcaggcggta acggccagcg ccgccccgcg ggaagtatcg tgccgcagtg taatgttgcg
     4561 gccgcatttg cagagcacca gcaccaacgg caggataggc agccagtagc ggccctgcaa
     4621 gccaaaaagc gtttggtagt tgatgggcgt ccagttcagc gccacgacga gcgacgacgc
     4681 cacgacgcag aggatgatgc ccaccacgcc gatcttggtg cggcggccca ggggggcttc
     4741 cgtcccctgc accggcagcg ccgctgccag cagcgccagc accaggccga cgcccagcag
     4801 ccagctgaca tcgatgcggt agacgatcgg ctcacccagc gtcgtgccca ggataccctg
     4861 cagccagagt gcaccctgct cagggatcgt gcgcagcaag agcttcgtcg tggcgggcag
     4921 gttgcggcag atatagccca gcgagaaggt ccagatggaa tcgccgttgg ggttggtcat
     4981 cagcagctgg tcaggttcca gcccgccgcc catgcgggaa agggcaaaca ccccgccgac
     5041 gacagccagc accaccgcgc agcccaggcc gcagcggaag aggcacagct ttttcgggtc
     5101 ggtgtgtact ttgtcataca gccaaagcac cagcccgacc gcagcggcca gcgccgcgac
     5161 gacgacgacg agcaccgcaa ggcttatatc gcgggcagcg tagaccagtt cgcccaagtt
     5221 ggcggagatc cacaggcaac ccgccagtat gagggcggcc gcctggatca gccgcccgga
     5281 gcgcaccctt gccccgaacg gtcctttgac aaattccgtc gaccccatga gattttccac
     5341 cgggataatg aagcagagca gcacgaccgg cagataaatt gccttggccg ggccgaccgc
     5401 cccgccgagc gcaatgagca ggattttttc ccaccacaca gcccgcttcc ggcgcagccg
     5461 caggcagagc gccatgtagc agaacaccat gcccagcacc gacgcatcgg gcgaaaggct
     5521 ggccgctaat tgcagcggca tcggcagcag cgccacgcag gccagcagcc cgcgcacccc
     5581 tgccggggcc agcgccacgg ccagcacagc cagcgtcaga tagaaaacaa ggttgaagat
     5641 acgggcctgg cgcagcatga cgtagaagcc cgccccgctg ctgcgggcca gccgcaggcc
     5701 cagcgcctgg ggcaggtagt taccactccc ctgcccggcc ttggccgtgc tggcggcggt
     5761 cagttcatcg gggccgcccg gctcctgttc gatggccagc gcctcggcct tgtaggcgag
     5821 aatgccgatc tcgccggtgc gggtgccaaa gtagggcgcg tcacactcgc ggaccagcag
     5881 attgccgtcg gcatcggcgg gctgttcacc acccaggcgg ctggcaagct cataggcagc
     5941 ggccaggtgg gtgtattcat cgggggcgac cagcgcgggc gtgacccgca caaacgccag
     6001 cccgcagagc aggcccgcca ccgcgaccgt gacagccagc ggcagctgca agcccgccaa
     6061 cagcacggcg gcaaaggcgc cccccgccag cagcggggcc agctcgttgg ccaactgcgt
     6121 cggccagctg ccggagtaat cggtcatata ttgcagcgcc agcgtggcgt tctggctttt
     6181 gccgtcggcg gtcagcggca ggtccttcac cgccgtctcg ctggcccaga ggcccagcgg
     6241 gccttcccac tccacggcgt tataaatgtg caggtagacc gtctcagcct gctccgccgt
     6301 gtagggggca ccgaaggaaa acccggtaaa gttatcgtca aacacgttgg cataattgcc
     6361 cgcagcctgg cgcagctcat tcccggcggc gtcgcacaga tcgaccatga ccatgcccgc
     6421 gcggtagagc tgcccgtggg tgctgaaccg cagccgcacg ccgtagagcg actccccggc
     6481 ttgcagggga atggcctggt caaatccttc ctcgggcagg tcgatgacct gggtgtagtc
     6541 atcgttgacg atctcataga ccggctggtt gccgacccgc agctgcaggt cgtacccgac
     6601 ccagcggcag agcagccaga ccgcagccgc cgccagcact gcagccgcga tactgccgac
     6661 gacgcggcgc agggagagtt gttttttcat aagcacaatg cctttctggc aggatgtaca
     6721 ggaatcagtg ttattataca gcatataggc aaaaaacgca aatcaccgct gcagctgata
     6781 gagataccag gtcacttcac cctcgccgcc gttgtagctg cactggtcga tgaccttgta
     6841 ctgcgggaaa tcgccgccca aatcaccggt cagcgcggcg acatagccaa ctgcgctgtt
     6901 caccagcacg gcctgctggt cactccactg gtcgtccagc ggcatattcg tcacgcagaa
     6961 atacttgcag ggcggaagca cccccgccgt cgtgtaaaag ccgccgtcca gcgtgccgta
     7021 gttgagcagc gatgcaccgt tgattttcgc agcaaagcga gtctggggca tcgtatcggc
     7081 atcacggaac cgcaaggcgt ggttggggct gaaggcaaag caccaggccg cccccgctgc
     7141 cagccacaga agaccgcaat ttctgcgcgt gccccaaagc gtcgccagcg gcacgatgcc
     7201 caacggcgcg aacacggcca gcacaaggcc gtagtagacg aggtagccgc ccatcaggct
     7261 tgtaagcgcc agccccgcag ccagtgcggc cacagccgcc accgcgctgg gcttgcgcgc
     7321 cgctgcggcc cagatcaaaa acattgtcag cagcaccgcc gcaggcaggg cgtcccgcac
     7381 ggcccaccaa aggtgctggg ccaaagccag tgccccgccg ccctctcctt tataaaggaa
     7441 gaggttatca taaaagtagc aggtgaacca gtcccccagc gcactgttta cgccgaagta
     7501 gatgacccac ggcagggtcg ccagcgccat gccgccgagg taggcggcca cgcttttgcc
     7561 cagctgtgcc gcccgcccac accataggta gacgacggcc agcaccacca cccaggcgag
     7621 gtagaacccc agcaccgtat acttgagcca gagggcacat ccggccagaa aaccgtgcag
     7681 cgccacggtg cgcagcggca tcgggtgggt gtcctgcagc gttttcaccg caccgtacag
     7741 cgcaaacgcc agaaacggca gcagcagttc ctcggcgctg ccgccgtggg caaaggcccg
     7801 gctggcggca atgcccgccg cgggcagcac ggcccaggcc gggtgcagtg cccgcccggc
     7861 cagcagttcc gctgtgcgca gaccgcagtg caaaaaaagc gcaaaggcga tgatttccac
     7921 caccagcacc ccggtgaagc cggtatagtc tatcaacgat gcaagaccgt acaagaagta
     7981 cagcaccggg cctttgtggt cgaacacatc ccgataaagc accttgccgc ggaacatcga
     8041 cttgcccatc gtgaagaaga tgttcgcgtc catccagtcg ttgaggacgt agagcggcga
     8101 ggatttgctg cacagcacca gggcaatggc tgcgataccc accagccacg ccgcgcgccg
     8161 tgcgttttgt tttgtaagca taggcagacc cttaacaaca aaccacgccc cggcggtaag
     8221 gccggggcgc ggaatggaac aattcaacta tttacacaag cgcgtaaatc agccgttctc
     8281 cttcatctta gcgaagcact cgctgcagta gaccgggcgg tcctcacgag gctggaaggg
     8341 aaccttgcag gccttgccac agctggcgca ggttgcgtcg aacatctggc gctcaccgcg
     8401 ggttgcgttc ttgcgggcat cgcggcaggg cttgcagcgc tggggctggt tctcaaaacc
     8461 acggctggcg tagaactcct gctcgccggc ggtccaaacg aactccttgc cgcaatcctt
     8521 gcataccaaa gtcttgtctt cgaacatagt gggtatctcc tctgtgttta ttttgcccgc
     8581 ggaagaatct aaaacaacgc atatggaatg gaccaactga actgatttta ggcgctactg
     8641 ggggcagatt gatatatgat gcaggcaaat cacctgaatc caaagttagg ggccgccgcg
     8701 cagcttgcgg cgggcgcggg ccagggcatt ggccacattt ttggcgggca ggcccaattc
     8761 ctgcgcggtc tgcgcggggg tcttgccctc cagcccggcc atgataacgc ggcgttccag
     8821 cacggaaagt tccgtctgca tccggcgcat cgtgtcggca tattgttcgc cggaaatcgc
     8881 gatctcttca ggacccgggc gggtgtcggt gctttcctcg gcgtcgggca ggggcacgct
     8941 gtaattcagc ggcgcgtgct ttttgcgcag cgcggctttg cgggcgtcct gctgggcgtg
     9001 gcggatgctg gccgccgcat aggcggtgaa agtctcgccg ctggcagcat cgaacccgcg
     9061 cactgcgtgg aacaggccga tcagcccttc ctgtacagca tcctcaaagt caagccccgg
     9121 ggcggtgcag gccgccgcgc cgctgcggat ggccggcatc atgcgggcaa tcagcgcagc
     9181 aagcgcattt tcgtccccct gctgtgcgcg cacaggcagg tcttgctcga tcgtggtctt
     9241 gggcagcggc atggactttt cctccgggaa gatgcgaaca atttaataat agcgggaaac
     9301 tgcgcatttg tcaatgtttt tttcgtcggt tcggcaggaa aaaggaaagg cttcccccct
     9361 tagggggaag ctggccgcga agcggactga tgaggggcag gtttgccatt attgcgctat
     9421 aacggcaaca ccgcccctca tccggcccgc gggccacctt cttcccaggg gagaaggctg
     9481 gagattttac agatagctca tctcttcttt ccgtcgcccg gccaggtcgg ggttatccag
     9541 cacggcaccg gcgcccaggg ccacacagtc cagtgggctt tcggcaatgt ggacatcgat
     9601 gccggtctcg ttctggatca gcgtatccag cccgcgcagc agcgcaccgc cgcccgagag
     9661 catgatgccc cggtcgatga tatcactgga cagttccggc ggcgtgcatt ccagtgtgtc
     9721 cttgatggcc tcgacaatgc gcagcagaca ttcactcatg gcgtcgcgga cttcctcgga
     9781 ccggatcagg atatccttcg gcaggccgtc cacgaggttg cggcctttga tctccatgct
     9841 ggtttccggg tcctgcgggc aggcagagcc gatctcgatt ttgatctgct cggccgtgcg
     9901 ctcgcccacc aggaggttat acttgcgctt gatgaaggca atgatgctct gatccagcgc
     9961 gtcgcccgcg cagcgcacgc tgcggctggc cacgatgccc gacaggctga tgaccgccac
    10021 ttccgccgtg ccgccgccga tatccacgat catgctgcca acagcgtcag tggtgggcag
    10081 cccgctgccg atggcagcgg ccatgggttc ctcaatgact gtgacctggc gtgcgcccgc
    10141 cttggctgct gcctggcgca cggcgcggcg ctcgacctcg gtgacgccgg acggcacgca
    10201 gatgaccacc cggggatggg caaaaatgct gttgccgcag gcctggcgga tgaagctttg
    10261 cagcatggct gcggtgatgt cgaaatcggc aatgacgccg tccttcagcg gacgcacggc
    10321 cacgatggac ccgggtgcgc ggccgatgac agcctttgcc tcatgcccga cgctgcgcac
    10381 ccgcagttcg tcgctgcgcg ggtcgacggc gacgacgctt ggctcccgca tgatgatgcc
    10441 ccggcccttc atgtagacca gcgtgttggc ggtgcccaga tcaatgccga tatccttggt
    10501 aaacatagcg tgtattctcc cgaaaaagcg tgtaaaaatg ttgcagaact tacccataca
    10561 gtctccccca ttccggggct gtgcgggttt actgaaattc gcgcagcagt tgtgccgtgc
    10621 ggtggaccgg cagccccatg atgttgtagt agcacccttc gatgcccgcg caccacagcg
    10681 ccgcgtgccc ctggatggca taggccccgg ccttatcgta gggttccggc gtgcggacat
    10741 aaatccgcag gtcgtcctcc tcgatggacg aaaaatggac ggatgttgtc tccaccgtag
    10801 cggcagcgcg gcggccccgg cagatgcaga cgccagtatg tacgcggtgc gcacgacctg
    10861 acagggcgcg cagcatccgc acggcgtcgg cctcgtcttt cggcttgccg aacacttcgc
    10921 cctcgcactc gaccaccgtg tcaaagccgc agacgatatc gtcggggtgt tcctcggcca
    10981 cagcgcgggc cttggcggta gccagggcct tggccaggtg tgccggggag tccgcggcaa
    11041 ttttcgtctc gtcgacgtcg ctggtgataa cggtatagtc cggcgtgata aggctcatca
    11101 gttcccgccg acgcgggctg ccggatgcaa ggatcaaagc catggggtgc ttcctttctg
    11161 gtttattata gtaggagttg gatgtcgaag tgtggcaaag tgggggtgga aaattattat
    11221 tttccggtcg acccaaagcc gccggcaccg cgctgggtgt cggtcagctc gtcggcagag
    11281 gtgaaggccg cctgccagac cggggcaatg cagagctggg ccacgcgctc gccgggctgg
    11341 atggtgtagg gttcattgga cagattcacc atcggcacgc ggagttcgcc gcggtagtcg
    11401 ctgtcaatga cgccgacgcc gtttgccatg cacagaccgt gcttgatgga caggccgctg
    11461 cgggcataga cgagggcgac gcactcgggg ccgggcagct cgatggcaag gccggtgggc
    11521 acgagggtac gctgcatggg tgtcagcgtc agcggttcat ccagcaccgc gcacagatcg
    11581 gccgccgccg caccgggggt ggcgtaggtt ggcacgaccg cgcggggatc gagtttttta
    11641 tattttacaa taggggtcat gggaacctcc aaaaaagata atagataaaa gataagagat
    11701 aaaaaagcag gtggattgcg gcactgcgtg ccgctcaaaa ttttattttt gccccgcagg
    11761 caaaaactcc acatcattta ttatctagta tctagtatct tttatctatt taattattcg
    11821 tatagtatag ccctctcgta aactcattgt caaacgggcg ggcgtccaaa agcagcgcca
    11881 aaatctggga aacgcgggcc ggggtctcga tggtaaaggc ggcaaccgcc acatccaccg
    11941 gcatttcgga caggcgctcg cccatataaa gcggcacggg gttgtacacc gtgcgcacac
    12001 ccgccccgcc gggggccgag caggtcacgg tgaagtcgcg gcccttgcgg tcgcgcaacg
    12061 tgccgccgcc ctggcatttg ccgcagtccc gcacgttgcg cagcggacac gcccgggtca
    12121 gcatcagcgg caggtggccg tagcacagcg ccgccgtggg cacgcccgga gccacagcct
    12181 gcatggccgt cagtgcggtc tcgggctgca aaagcatccc ctcgatgccc aggctcttat
    12241 accgcgcggc gctcatcggg ttcgtcacgt tcagacccag gccgccgtac agcgtccagc
    12301 cgtcggtgta gcgcagctgt gcggggttat tgaccaccgc gcccgcaaaa cctgcatcct
    12361 gcaaggcgtc cagccgggcg gcggtttttt gttccagctt gccgaacatg acgcggggca
    12421 gttccagcag cgttttctcc cgccatgcct cgggaatgct ctcggcctcg gcaatcggga
    12481 aaatcaggta tttcagcttt tccacagcat ccgccgggca ctgggccgca ttggcaaacc
    12541 gcgcggccag taccggggcc tgcccgacag tgtgcgccgg gtaggcgggc agcgtgatat
    12601 cctgcgtggc gtggggtcgg actttgctgc gcttttccag cagcttatcc aatgcttcgc
    12661 ggcgcagctc gttccaggca gaaccgggca gaaagcctaa gcccccctct cctgcttcgg
    12721 tgcgcaggtc ggccatgaca aagggcgtgc cgccggtctt gcccagtgcg cgctcaatgc
    12781 cgggcagcgg gtccttctgg gcaggctgcg gctcatcggc gctgtacacg accgccttat
    12841 ggccgtcgtc gtcccaggcc gtcagcttga tgccgccgtc ctcgacgccg ccgctgacct
    12901 cgtaatgcac cggcacgcgc tgcaattcac ggcggaacaa ctcgcgggcc ttgggcgtcg
    12961 ctgcgcgggt cgccgcggcg tccgcctcgg tgcgcacgcc gaacattttg ccgtcgttgc
    13021 ggttcgtcag gtagccgtcc gtgaaacccg agcgggagaa gatatcccgc acgagctttt
    13081 catcgtaagg ctggcccgca cagaccgcac ggcaggccgt gacgaccgcc gccgcatact
    13141 cgggcgtgcg caggcggccc tcaattttca cgctggcgac acccgcgtcc atcagctccc
    13201 gcaagtgcgg gatgtagtcc atatctttca ggctcaaatg gcaggccttg ccgggctggc
    13261 cgggtttcag gcctgcgctg gcgtcaaagg gcaggcggca gggcccggcg caggcgcccc
    13321 ggttgccgct gcggccgccc aaaaaggcgc tcatcatgca ctggccggac acactcatgc
    13381 agagtgcgcc gtggacgaac acctccacct caatggggct ggcctcgcag acggcccgga
    13441 tttcatcgag ggacaactcc cgcgcgagaa tgacgcggtc ataccccagg gcggccaact
    13501 ctttggcccc ttccggcgtg tggacgctca tctgggtcga gccgtgcagg tgcagcgtcg
    13561 gtgcaatgct cttgaccagc tgcgccgtgg caagatcgtc ggcaatcacg gcatccgcgc
    13621 ccgcttcggc cacagcccgc accgcgtcgg ccaggcccgc cagttcgcgg tcatagacca
    13681 gcgtgttcag cgtgacatgg accttgacgc cgcgcgcatg gcagaaggcc acagcctccc
    13741 gcagttcatc gggggtaaag ttgcccgccg tgcggcgtgc attaaagccc gtcagcccca
    13801 gataaacagc atcagcgccg ctgaaaacag cggcgccaag catttcgcgg ttgcctgccg
    13861 gggccaggat ctccgggcgg ctcattcgtc ctccaccggg aactggctgc ggcgcaggcg
    13921 gcgttccagc tcatcgatgc ggcgcttcag cgaggcattc tcacggcggg cttcctcggc
    13981 agccatcttg gcgctggccg cgtcctcgag ataatccttg atctggcggc gcatattgtc
    14041 cgcgctgccg ctggcctttt ccagctcgtc gcggtagttc agcgccgtca tgaccgccgc
    14101 cgtggtgata gacacagagt tcgaggacgc catcagctcg ttcatgtcca ggttcagcct
    14161 gtcggcgagg ttctgcatat agtcctcgct ctcgctcgtg ttgactacat agctggaccc
    14221 gcagatgttc agccgcactt ttgaggtagc catacccata atcatactcc ttccgatcct
    14281 gcctgtgccc ggctttggcg gcccgggcgc ataaatccac aattattata atacaaatcg
    14341 gcggtttaca aaagcccttt ttgaggaaaa tgtataaaaa agatgcaaac gggcaatttt
    14401 cccttgcgca atgagccaaa tatcgttata atcaatagtg tggtttattg accccaccca
    14461 aaaacagtcg caagaatctg ttatcccagt gattttatca cagcgaagaa ggagagaacg
    14521 attttgaaat acacaaagcg tgaatttgag aagctgctca aagataagct gatgagcgag
    14581 tgcaacgtca ctttggacac tgcctctgct gaccagattt atcgctgcct ggctatgatc
    14641 accc
//
LOCUS       DBMDSQ010000020        14630 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_1291_length_14630_cov_2.247505, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000020 DBMDSQ010000000
VERSION     DBMDSQ010000020.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 14630)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 14630)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14630
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1291_length_14630_cov_2.247505"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..785
                     /locus_tag="ACZPAX_01520"
     CDS             <1..785
                     /locus_tag="ACZPAX_01520"
                     /inference="COORDINATES: protein motif:HMM:NF012464.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HIR1112609.1"
                     /translation="EIQALHFNTIITENPNWTLYKIYSDIASGTRKSGRSGYCEMLRD
                     AKRHRFDYIFVKSLSRFGRDTLEALSTIRRLKAMNIGLISEVERINTLKIDDATLSIL
                     FAVAQEESATKSENIKFGIHQRMREGKALLNHTRFLGYTKDAEGQLVIVPEEAEIVRK
                     IFSLYLEGNGVRKIKRYLEEHGIRTVTGKSEWSTSTIDRMLSNEKYMGTLLLQKTYTP
                     DFLTGKQVKNRGELIMYMVENAHEAIVEKKVFEAVQAKKKKR"
     gene            1199..2845
                     /locus_tag="ACZPAX_01525"
     CDS             1199..2845
                     /locus_tag="ACZPAX_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008399865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding domain-containing protein"
                     /protein_id="HIR1112610.1"
                     /translation="MTEQELRKMIAAGENTRQEFKSWVKCGNYKQRKELAVKSTVALA
                     NAVGGVFLFGVEDDGTITGCSESDPQALMEAIYDMTRPSLFTEIEAVETSDGVVLVVS
                     VDKVGSPVATTSGIYYKRLGKVTKPFYPANDVYSPADNLDFSSKIVEGASEGDIDLLE
                     VYRLKEKLRIRDSASALPALADTTFLDNLKLIRLEKDEVRVTVAGLLFVGKTTSISRL
                     LPQAEVIYLHYSDEAQTEYDNRMDMQEPLISILDKLTERIRTYNHLTNVQVGLYRLEV
                     YDFSEAVFQEALLNALAHRSYEETAPIYVKHYPTKIVIENPGGFPEDINESNIITHQS
                     IPRNKLIAMTLQHLKYVQRSGQGVDIMYQSMLTEGKPYPEYTSTPNSVRLTLRSTMEN
                     QNFVHFIAEMQDKRSKMFTLSELMILHYLREHQKITLKQAAKTIQESDNNAHKVLNSL
                     RDEGLLELNGKTYMLTLKVYETVKTDVAYVQDKTVNQIQAKGRIVEYLQLKPWITNQK
                     AQELCGFNKNQTYYILRQMCKDGILQPVGKCRGTKYKINK"
     gene            complement(3116..3754)
                     /locus_tag="ACZPAX_01530"
     CDS             complement(3116..3754)
                     /locus_tag="ACZPAX_01530"
                     /inference="COORDINATES: protein motif:HMM:NF024296.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy0128 family protein"
                     /protein_id="HIR1112611.1"
                     /translation="MSFGKTTLRRWLPAAALALALLCPLPVFAAQAGAEIPVTVRTDG
                     AACDTVYTVEITPLDNAPTPAQTSVQVKGGGTVYFTGLTFDAPGDYRYKLAQVKGCAA
                     DTTYDGRTYTVTVRVMTAADGSLDTELWAVRSGRTAKAAGVVFTNRYDPPAPAATATP
                     APSAAPMPRPIKNTARPVGALPRTGDAFPLEALAALLCAGVVGFGTAWNKRR"
     gene            complement(3751..4518)
                     /gene="srtB"
                     /locus_tag="ACZPAX_01535"
     CDS             complement(3751..4518)
                     /gene="srtB"
                     /locus_tag="ACZPAX_01535"
                     /EC_number="3.4.22.71"
                     /inference="COORDINATES: protein motif:HMM:TIGR03064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0016485 - protein processing [Evidence
                     IEA];
                     GO_process: GO:0045184 - establishment of protein
                     localization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class B sortase"
                     /protein_id="HIR1112612.1"
                     /translation="MLSAAAHLARLGSAVVSALAAVLMAVLLAFGGFALWQDAAVSRG
                     AFASADLLQYKPADKNTAAPTLAELQALNPDVCGWLTIDGTHIDYPLVQGATNMDYIN
                     RDVYGEFSLSGAVFLDSRSKADFSDGYSIVYAHHMENGAMFGDISKFVQADYFAAHPS
                     GSLILPDAAYEIELFVCLEADAYDAEIYAVERYTADTAALLDAVQARAVQQRALDVGS
                     QDRLIALSTCAGAETNGRVVLLGRLRPATLDEGDETQ"
     gene            complement(4518..5606)
                     /locus_tag="ACZPAX_01540"
     CDS             complement(4518..5606)
                     /locus_tag="ACZPAX_01540"
                     /inference="COORDINATES: protein motif:HMM:NF049299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF7601 domain-containing protein"
                     /protein_id="HIR1112613.1"
                     /translation="MNTIKLSAALTAAVLAGALSTAAYAADAERPASRITVSGGSIIL
                     KKAINLDKAPGAGGIKGTITFNVAAGQDGDLPQAHTADEMLGTADQLASTTATAEFTA
                     DAQGKANTESNVTVDFNMDKFTKPGIYYYRLTEQAHGISGLSTAPLYLLKVSVANESE
                     AEPDGQHFKLEYAILAEKETGTKAELVTNEYTTHSLTVTKRLAGDFASYGDSFDFTLH
                     ITDPDTENHMASVTLRTGPADDELSGPGTVYTLENGKKEIKVTLKGNEQLEVTGLPEG
                     ATYTIDESGTDAAKYTSTTWVFPGGTGKGTSLGQQTIGTADAAITVTNIRDAVTPTGL
                     LLDAAPYGAMLALAVGSGAVVFRKRRCD"
     gene            complement(5678..6193)
                     /gene="lepB"
                     /locus_tag="ACZPAX_01545"
     CDS             complement(5678..6193)
                     /gene="lepB"
                     /locus_tag="ACZPAX_01545"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HIR1112614.1"
                     /translation="MEPQTRRRAAWADIREFLVRAGALAALLVMLFGVVFGLYIQPDA
                     AMHPHLKPGDLLLFYRLPRSCTAGEVVVFTKDGQRRTGRVAARGGDTVEVTDAAALVI
                     NGSTVAEPDIYEETPKYDSNVTYPLTLADGEYFILCDARAGARDSRWFGPVQAGEIQG
                     RVIAVLRRNGI"
     gene            complement(6197..9052)
                     /locus_tag="ACZPAX_01550"
     CDS             complement(6197..9052)
                     /locus_tag="ACZPAX_01550"
                     /inference="COORDINATES: protein motif:HMM:NF024296.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF7601 domain-containing protein"
                     /protein_id="HIR1112615.1"
                     /translation="MRKRLLAVFLACCMAASTASTVFAADDLPALTELPAVAAPADPD
                     PPAAEEVVVETEVPDPTAEPAPETTEPPPDSAAEPTPEPVPTEAPAPEPTAETTPEPT
                     AEPAPEPTEPPTPDPTAAPTQAPAGPEPTAAPTPQPTVPPAPTASPTPLPTASPVSQQ
                     TAEETEYAAVLAAPIPEPTAVPLVVDTLEVEDTISTDGCFTAVVNGSAERQDVTYTWY
                     RSHDGQNWEQVTPRICTGSEWNITEGSEHKLNAAIDSCIANVGPTERLYYKVEVTGFN
                     GTKAAEARVPYHLQLQNGSFESPSLHSMAVEHTRTGVLSYAPEDKEQTIKSHFAQLAD
                     TTQGLVWHTTGLYYHWTDEEETRADKTQWANYIEIVDGTNHNYNDDLENNDPRVSYNT
                     RSAQDGNQFAELNCEAYGALYQDVLTVPGSTLNWSLAHKGRNGADTMVLLIAPVTVAE
                     AITKTLTAAAQNEAKDSVRNALNDLVEYNGQKVPISTFMVGGEMTDGIANWATHKGTY
                     QVPGGQYVSRFFFLAVQTQDDKRTEGNLLDNVWFSTDPAPAVAGRANLTIRKTITGGL
                     TVEELTAARANLTFTVANQNGTVATIHGADMTVDSADPTRSRYTLTDLPLTSDDGSVN
                     YTYTVEETGHDAPAGLLYLGTQAAVNGDNEQTGTSLADITLTTSADTTAEFRNIYARP
                     TGSLHLTKAVADEAQQAEADAVTNTFTVSSLPIGSYTLQFDDGTTETRELTAGGALTI
                     ALKGLTGVTLKDIPVNRYTVTETAHPDLANYYCTTTADDAAAEVQVMSGQTAEVTITN
                     SYAPFLTLTITKQVTGGLGDARREFPFTATVAGQAITAASAYVTKGEGAALTGSGFTL
                     RHRGSITIGHLRPGDAFTVTETDNTGYETSFDLDGSVEVGASGGGTLGAQSATLTCVN
                     NKDGAPPTGFGPDTAPAVWMLLCALLGLVCLRRRKGA"
     gene            complement(9235..10914)
                     /locus_tag="ACZPAX_01555"
     CDS             complement(9235..10914)
                     /locus_tag="ACZPAX_01555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1112616.1"
                     /translation="MRKHYERILHDSESRVQRYLDIQILDENSPFCGGFPKADSLAEP
                     KTAIYRATTMTACYLNPESRYYLDAELGRRLALALAFVRRSQRPGGYFDLINCNFFSG
                     PDTAFCTKRLLPAYVYLCKVVDGALPAAPEAKAAAAELKPKYEAIIRDAADALCHCGF
                     HTPNHRWAIASVLMLCAKLFDKPECRTAAEAILKEGNDCNEDGEYAERSAGNYNRINN
                     DAMIMLAVATGDESYYAPVLRNLEMMLTYIDPDDSIFTNNSTRWDMGKKIYPREYYLE
                     YLYMGYTCRKPELLDAANAIMQMVERHALRSFDCLINLMLLPELAALEHEGSATPTNY
                     HKYYEGSGIVRCRRGSWSYTILNNSPSFLFFQHGDFTLTVRIGASFCEHRNFVPAALA
                     PKDGGYALHQTMTGWYYLPFAEKPATSDWWQMDNAHREKLYGPDMVFDVQVNEVENGL
                     DIHIVNSGIDRAPLRVELAFNAGCRVENEHFMLEGNDGGGVVVKDGTLVASKGVNGIS
                     VGPCFAEHNYTAGKEGSMGRMDGCFTAYLTAYSTFDRTISLRAVPSKYDQA"
     gene            complement(10928..11884)
                     /locus_tag="ACZPAX_01560"
     CDS             complement(10928..11884)
                     /locus_tag="ACZPAX_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009322429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HIR1112617.1"
                     /translation="MRYCIGIDLGGTNIAGGIADLDGKLLLTDSIKTGLPCPASDIAD
                     RIALLCRRMATALGLALSDAAWVGIGAPGSVDVDTGTVLYANNLEFDNVPLGQMIHER
                     TGLPVYVDNDANVAAWGEYCAGAGKGSSSMVMVTLGTGVGGGVVLNGKLLTGCNGAAA
                     ELGHFVIDMHGKECNCGMRGCFEQYGSVTALICQAREAMAAHKESKLWALAENEEANV
                     NGKVILAAYDAGDETAVQVVNTYVHYLCVGVTSIINIFQPEVLCLGGGISRRSDVLVE
                     PIRAFNAAHGYGRSCPKQPRIMSAELFGDAGIIGAALLGKGK"
     gene            complement(11907..13076)
                     /locus_tag="ACZPAX_01565"
     CDS             complement(11907..13076)
                     /locus_tag="ACZPAX_01565"
                     /inference="COORDINATES: protein motif:HMM:NF019310.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR1112618.1"
                     /translation="MTKKWSSRLTFFAFVVLMVGLGSNDSLRGIFSTIFQEHFSLTTT
                     QLGLIVTASYIGNLVFLLVGGNLSTRFSKKRVLQVLILIWMAALALFAFTSNYTVLLI
                     GMALALGSSTLLNTTMNLITPLLFATAPGFFVNFLFFTQEIGTSGSQYILGSHADGFA
                     FWQHTNLVLLILGAVAFVLLLFCNVPEEAAAPADTPAQKGGYDWRIIVPYVLVFGFYF
                     IAEHGVMNWMVAYGVDGLGLPQASAAKYLSVFFGGMMIGRLCLSPLVDKLGALKSLAA
                     FGGVSCVLYLIGSLGGAVTMPVWAISGLSFSILYPTLVMSIRLYFPAQQVSGAAGTIL
                     SIASLADILFNVGFGKLVDMTGYAVSIRVLPLSALAFFIVFILFTKFCKPTQKLK"
     gene            complement(13076..14164)
                     /gene="bglB"
                     /locus_tag="ACZPAX_01570"
     CDS             complement(13076..14164)
                     /gene="bglB"
                     /locus_tag="ACZPAX_01570"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004556 - alpha-amylase activity [Evidence
                     IEA];
                     GO_process: GO:0009313 - oligosaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase BglB"
                     /protein_id="HIR1112619.1"
                     /translation="MQKADIENKLELCIESFKSVLYERDETFLENMAKSNMDQGNLAR
                     YMLWEWTQGVGLYGLWKLFEKKHDQRCLDVLTEYYDARIAEGLPSKNINTMAPIFALS
                     YLYEYTKNEKYGDICKEWAEWAMHGLPRAGEGGFQHVTSDRVNDGELWDDTLFMTVLP
                     LANIGRILGRQDYIDEAVYQYLVHTKYLADKKTGLWYHGFCFNGCHNFAEAFWGRGNS
                     WVTAAIPLLLEIVPVGNPDRRILENALLRQIESLAKYQDEGGMWHTLIDDPTSYLEAS
                     ATSGFGFGILKAVEQGIVPESYRANAMRALQPVLDCIDEKGVVQQVSYGTPMGRESKD
                     FYKQIPLHPMPYGQAMAILFLMEAEAQV"
     gene            14340..>14630
                     /locus_tag="ACZPAX_01575"
     CDS             14340..>14630
                     /locus_tag="ACZPAX_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009322425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1112620.1"
                     /translation="MYLDDALLYNQGYMISINDLDSPLFYLFDYDSRSFKINMSFQHF
                     HQFHEIHILLSGRASHLIEGDYYTLQPKYMTGLLDINAGRKAARFVRFCDAFN"
BASE COUNT         2950 a         4143 c         4348 g         3189 t
ORIGIN      
        1 ttgaaattca agcactccat ttcaatacaa ttatcactga aaacccaaat tggacgcttt
       61 ataaaattta cagcgatatt gcgtcaggca cacgaaagtc tggccgcagc gggtactgcg
      121 aaatgttacg cgatgcgaag cgacatcgtt ttgattatat attcgtcaag tcattgagcc
      181 gcttcgggcg agatacgctt gaagctctat caaccataag gaggctgaaa gctatgaata
      241 ttggacttat ctctgaagtt gaacgaatca acaccttaaa gatagatgat gccacgctct
      301 ctattttatt tgccgttgca caggaagaaa gcgccacaaa gagcgagaat atcaaatttg
      361 gtattcatca acgtatgcgc gaaggaaaag cacttttgaa tcacacccgc tttttaggtt
      421 ataccaagga tgccgaaggg caacttgtaa ttgtgcccga agaagccgag atcgtccgta
      481 agatattttc gctgtattta gaaggcaacg gtgtccgcaa aattaagcgg tacttggagg
      541 aacacggtat aagaacggtt acaggaaaat ccgaatggag tacatcaacg attgaccgta
      601 tgctaagcaa tgagaaatat atgggcactc tgttactgca aaagacctac acaccggatt
      661 ttttgactgg gaaacaggtg aagaatcgtg gagaactgat tatgtacatg gtagaaaatg
      721 cacatgaggc gattgttgag aagaaagtat ttgaggcggt acaagcaaaa aagaaaaagc
      781 gataattcat agtttgttct caacttctat tccctgatgt gctatactat tattgagcca
      841 agattcttcc cctccttggc tcaccagatc cgctaggctc cgataggata aaagcaaatg
      901 agaaggttgt tgcaaaagga ggggatgcac tgatgtctaa aattatcgta cgaattcaac
      961 tttttctcga taaggtgttc ggtcataaat ataccggttt tactataaag gctccattca
     1021 tcaacttctc atttgcgcct acgaaactta tacagattgc cctaacagac actgactttt
     1081 tcatagggtg gtttatcaca tagagataag gcaacttgcg taagtgagtt gccttatttg
     1141 ttttatatgc tataatatgg ttatcatctt tttttgcttc tgagggagag gagaagccat
     1201 gacagaacaa gagcttcgaa aaatgattgc cgctggcgaa aacacccgac aagagtttaa
     1261 gtcatgggtc aaatgcggca attacaagca gcgaaaagaa cttgcggtga aaagcacggt
     1321 tgccctagcc aatgcagtgg gcggcgtttt tctgtttggt gtcgaagatg acggaaccat
     1381 tactggatgt tcagaaagcg atccgcaggc tttgatggaa gctatctacg atatgactcg
     1441 tcccagccta tttaccgaaa ttgaagcggt agaaacatct gacggggttg ttttggttgt
     1501 gtctgttgat aaggtaggct cccctgtggc aaccaccagc ggtatttact ataagcgact
     1561 aggaaaagta actaaaccat tttacccagc aaacgacgta tactcacccg ctgataatct
     1621 ggatttttca tccaaaatcg tagagggtgc ttccgaaggt gacatagatt tgctggaagt
     1681 atatcgctta aaggaaaaat tgcggattcg cgattcagct tccgcgttgc ccgccctggc
     1741 agatacaact tttctggata acttgaagct cattcgcctt gaaaaagacg aagtgcgtgt
     1801 aactgtggct ggtttactgt ttgtgggtaa gaccacatct atttcacggt tgttgccgca
     1861 ggcggaggta atttatctcc attacagcga cgaagcacag acagagtacg acaaccgaat
     1921 ggatatgcaa gagccactta tttcaatttt agataagctg acggaacgta tccgaaccta
     1981 taaccacttg accaatgtgc aggttgggct gtatcgtctt gaggtttatg atttttcgga
     2041 agccgttttt caagaagcgt tgttgaacgc cttggcgcat cggtcatacg aagagaccgc
     2101 cccaatttat gtcaagcatt atccgacaaa aatcgtgata gaaaaccctg gcggttttcc
     2161 agaagacatc aacgagagca atattatcac acatcagtct attccacgca acaaactgat
     2221 tgctatgacg ctccaacatc tgaaatatgt gcagcgttca ggacagggcg tagatattat
     2281 gtaccagagt atgctgacag aaggcaagcc ttacccggag tataccagta cgccaaattc
     2341 tgtaaggctg accttgcgaa gcacaatgga gaatcaaaac tttgtgcact tcatcgcgga
     2401 aatgcaggat aagcgttcaa aaatgtttac cctgtcggaa ctgatgattc tgcactatct
     2461 acgtgaacat caaaaaatca ccttaaagca agcagcaaaa accatccaag aatccgataa
     2521 taacgcccat aaagtattga atagcctgcg agacgaggga ctcttggaat tgaatggaaa
     2581 gacctatatg ctgacactca aggtctacga aacggtaaaa accgacgtag cttatgttca
     2641 ggataaaaca gtcaatcaaa ttcaggctaa ggggcgaatc gtggagtatt tgcaacttaa
     2701 gccatggatc acgaaccaga aagcgcagga gttgtgtggc tttaataaaa accaaaccta
     2761 ttacattctg cggcagatgt gcaaagatgg catattacag cctgttggca aatgccgcgg
     2821 aacaaagtac aaaatcaaca aatgataatt tacgctgact ttttgaattt acgctgactt
     2881 ttacgctgac tttttgaatt tacgctgact tttacgctga aaaaatgctg cctttttatc
     2941 tgtgatgtat tttcatcatt tatagtcttc ccattgtaac gggtatcttt tttgtaacat
     3001 agaaacaagg tcacttgcat ttgcaagtga ccttttttgt ttattatagt caggtttccc
     3061 ctgcaaagca agtgccccgg cagccgcaaa ctgccggggc cttccttatt acatactatc
     3121 tgcgtttgtt ccaggccgtg ccaaagccca ctacgcccgc gcagagcagc gcggccagtg
     3181 cctccagcgg gaaagcatcc ccggtccggg gcagggctcc caccgggcgg gccgtgtttt
     3241 ttatggggcg cggcatcggc gcggcgctcg gcgcaggtgt ggccgtggct gccggggcgg
     3301 gcgggtcata gcgattggtg aacaccaccc ccgccgcttt agcagttctg ccgctgcgca
     3361 ccgcccacag ttcggtatca aggctaccgt cggcggcggt catcacccgc accgtcacag
     3421 tgtaggtgcg gccgtcgtag gtcgtatcgg cggcgcagcc ttttacctgg gccagcttat
     3481 agcggtaatc ccccggcgcg tcaaaggtca gaccggtaaa atacaccgtg ccgccgccct
     3541 tgacctgcac gctggtctgg gccggtgtcg gggcgttatc cagcggggtg atttccaccg
     3601 tgtacaccgt gtcgcaggct gcgccgtcgg tgcgcaccgt caccgggatc tcggcccccg
     3661 cctgtgccgc aaagaccggc agcgggcaga gcagcgccag cgccagcgcc gccgcgggca
     3721 gccagcgccg cagggtcgtt ttgccaaaac tcattgtgtt tcatctcctt catccaaagt
     3781 tgccgggcgc agccgtccca gcagcaccac acggccgttc gtctcggccc cggcgcaggt
     3841 agacagggcg atcagcctgt cctgcgagcc cacatccagc gcccgctgct gcacagcccg
     3901 ggcctgcacc gcgtcaagca aggcggcagt gtcggcggtg tagcgctcca cagcgtaaat
     3961 ctccgcgtcg taggcgtctg cctccaggca gacaaacagc tcgatctcat acgctgcatc
     4021 gggcagtatc agactgccgg aagggtgcgc cgcaaagtag tctgcctgca caaactttga
     4081 tatatcgccg aacattgcgc cattctccat atggtgggcg tagacaatgg aatacccatc
     4141 gctgaaatcc gccttgctgc ggctgtccag aaacaccgcc cccgacagcg aaaattcgcc
     4201 gtagacgtcg cggttgatgt agtccatatt cgtcgcgccc tgcacaagcg ggtagtcgat
     4261 gtgggtgccg tcgatggtca gccagccgca gacgtcgggg ttcagcgcct gcaattcggc
     4321 cagtgtcggg gcagcagtgt tcttgtcggc gggcttatat tgcagcaggt ccgcgctggc
     4381 aaatgcgccc cggctgacgg cagcgtcctg ccagagggcg aacccgccga aagccagcag
     4441 caccgccatc agcacggctg ccagggcgct gaccaccgca ctgcccagcc gggccaggtg
     4501 tgccgctgcg gaaagcatca atcacagcgg cgcttgcgga acaccaccgc accgctgccc
     4561 accgccaggg ccagcatagc gccgtagggc gcagcatcca acagcaggcc ggtgggcgtg
     4621 accgcgtcac ggatgttcgt gaccgtgatg gccgcgtctg ccgtgccgat ggtctgttgg
     4681 cccaggctgg ttcctttccc ggtgccgccg gggaacaccc aggtggtcga ggtatatttc
     4741 gccgcgtcgg tgccgctctc atcgatggta taggtcgcgc cctcgggcag gccggtcact
     4801 tccaactgct cgttgccctt gagggtcacc ttgatctcct tcttgccgtt ctccagggta
     4861 tacaccgtgc cggggccgct cagctcgtcg tctgccgggc cggtgcgcag cgtcaccgac
     4921 gccatatggt tttcggtgtc aggatctgtg atgtgcagcg taaagtcaaa gctgtcgcca
     4981 tagctggcaa agtcacccgc cagccgcttg gtcacggtca ggctgtgggt cgtgtactcg
     5041 ttggtgacaa gctcggcttt tgtgccggtc tctttctcgg ccaggatggc atactccagc
     5101 ttgaagtgct gtccatccgg ctcggcctcg ctctcattgg cgacgctgac cttcagcagg
     5161 tagagcggcg cggtggacag gccgctgatg ccgtgggcct gctcggtcag acggtagtag
     5221 tagatgccgg gctttgtgaa cttgtccata ttgaaatcaa cggtcacatt gctctcggtg
     5281 ttggcttttc cttgggcatc ggcggtaaat tccgccgtgg ccgtggtact ggcaagctga
     5341 tccgccgtgc cgagcatttc gtccgcggtg tgggcctggg gcaggtcgcc atcctggcct
     5401 gcggccacgt tgaaagtgat cgtgcccttg atgccgcccg cgccgggggc tttgtccagg
     5461 ttgatggcct ttttaaggat gatgctgccg ccgctgaccg tgatgcggct ggcaggtctt
     5521 tcggcatcgg ctgcataggc cgccgtggac agcgccccgg ccagcacagc agcggtgaga
     5581 gcggcagaaa gtttgatggt gttcatggta agtacctcgt ttttatgtga tggatggctc
     5641 ggaaggaaag gcggacggca acggcaaggc cgccccttta tatcccattc cgccgcaata
     5701 cagcgatcac gcggccctgt atctcccctg cctgcaccgg gccgaaccag cggctgtcgc
     5761 gggcgcctgc gcgggcgtcg cataatataa aatattcgcc gtcggccagg gtcagcgggt
     5821 aggtcacgtt gctgtcatat ttcggcgttt cttcgtagat atcaggctct gccaccgtgc
     5881 tgccgttgat gacaagcgcc gctgcgtcgg tcacttccac cgtgtcgccg ccccgggccg
     5941 cgacccggcc tgtgcggcgc tggccatcct tggtaaagac aacgacctcc cctgccgtgc
     6001 agctgcgggg cagccggtaa aacagcagca ggtcaccggg cttcaaatgc ggatgcatcg
     6061 cggcatcggg ctggatgtac agcccgaaca cgacgccgaa cagcatgacc agcagcgcgg
     6121 ccagcgcccc ggcccgcaca agaaactccc ggatatccgc ccaggctgcc cggcgccggg
     6181 tctgcggctc cataggtcac gcccccttgc ggcggcgcag gcagaccaag cccagcaggg
     6241 cgcagagcag catccagact gccggggccg tgtccgggcc aaatcccgtg ggcggtgcgc
     6301 cgtccttgtt gttgacgcag gtaagcgtgg cgctctgtgc gcccagggtt ccccctccgc
     6361 tggcgccgac ctcgacgctg ccgtcaaggt cgaagctcgt ctcgtaaccg gtgttgtcgg
     6421 tctcggtcac ggtgaaggca tcccccgggc gcaggtggcc gatggtgatg ctgccccggt
     6481 ggcgcagcgt gaagccgctc ccggtcaggg ccgcgccttc ccctttcgtc acataggcgc
     6541 tggcggcggt gatggcctgg ccggcgaccg tggccgtgaa gggaaactca cggcgggcgt
     6601 cgcccagacc gcctgtcacc tgcttggtga tggtcagcgt caggaacggc gcatagctgt
     6661 tcgtgatggt cacctctgcc gtctggccgg acatgacctg cacttcggct gcggcatcgt
     6721 cggcggtggt cgtgcagtag tagtttgcca ggtcggggtg ggccgtctcg gtcacggtat
     6781 agcggttgac cgggatgtct ttcagcgtca cgccggtgag gcccttcagc gcaatggtca
     6841 gcgcgccgcc cgccgttagt tcccgcgttt cggtcgtacc atcatcaaat tgcagtgtat
     6901 agctgccgat gggcaggctg ctgaccgtga aggtgttcgt cactgcgtcg gcttccgctt
     6961 gctgcgcctc gtcggccaca gccttggtca ggtgcaggct gccggtgggg cgggcgtaga
     7021 tgttgcggaa ctctgcggtc gtgtcggcgc ttgtggtcag cgtaatgtcc gccaggcttg
     7081 tgcctgtctg ctcattgtca ccgttcaccg ccgcctgggt gcccaggtag agcaggcccg
     7141 ccggggcatc gtggccagtc tcttccacgg tgtaggtgta gtttacgctg ccgtcgtcgc
     7201 tggtcagcgg caggtcggtc agggtatagc ggctgcgggt ggggtcagca ctgtccacgg
     7261 tcatatccgc accgtggatc gtcgcgaccg taccgttctg gttcgccacg gtgaacgtca
     7321 ggtttgcccg cgcggcggtc agctcctcga cagtcaggcc gcccgttatg gttttgcgga
     7381 tggtcagatt tgcgcggccc gccaccgccg gggcagggtc ggtgctgaac cagacgttgt
     7441 ccagcagatt gccctcggtg cgcttgtcgt cctgggtttg caccgccagg aaaaagaagc
     7501 ggctcacata ctgcccgcca gggacctggt atgtgccctt gtgtgttgcc cagttggcga
     7561 tgccatcggt catttcgccg ccgaccataa aagtgctgat gggtaccttc tggccgttgt
     7621 actcgaccag atcgttcagg gcattgcgca cactgtcttt agcctcgttc tgggcggcgg
     7681 cagtcagcgt tttggtgatt gcttcggcca ccgtgaccgg cgcgatcagc agcaccatcg
     7741 tgtccgcgcc gttgcggccc ttgtgggcca gcgaccagtt cagcgtggag ccgggcacgg
     7801 tcagcacatc ctgatagagc gcaccgtagg cttcgcagtt cagctcggcg aactgattgc
     7861 cgtcctgggc gctgcgggtg ttgtagctga ccctggggtc gttgttttct aagtcgtcat
     7921 tatagttgtg gttggttcca tctacaattt ctatgtagtt ggcccactgg gttttatcgg
     7981 cgcgtgtttc ttcctcgtcc gtccagtggt agtaaaggcc tgtcgtgtgc catacaagcc
     8041 cctgtgtggt gtccgccaac tgggcaaaat ggctttttat ggtctgttct ttgtcctccg
     8101 gtgcatagga aagcacgccg gtccgggtat gctccaccgc catgctgtgc aggctcgggc
     8161 tttcaaagct gccgttttgc agctgcaaat ggtagggcac acgggcttcg gccgccttag
     8221 taccgttgaa gccggtgact tccactttgt aatacagccg ctcggtaggg ccgacattgg
     8281 cgatgcagga atcaatggcg gcgttcagct tgtgctcgct gccctcggtg atgttccact
     8341 cgctgccggt acagatgcgc ggcgtgacct gctcccagtt ctggccatcg tggctgcgat
     8401 accaggtgta ggtcacgtcc tgccgttccg cgctgccgtt gacgacagcg gtaaagcagc
     8461 cgtctgtgct gatggtgtcc tctacttcca gcgtgtccac tacaaggggc acggccgtcg
     8521 gctctggtat aggcgctgcc agcacggcgg catattccgt ctcctccgca gtctgctgtg
     8581 aaaccgggct ggccgtgggc agcggtgtcg ggctggccgt gggcgcgggg ggcacggtag
     8641 gctgcggcgt tggggccgcc gtcggctccg ggccggccgg ggcctgggtt ggcgcggcgg
     8701 tggggtccgg ggtgggcggt tccgtcggtt cgggggcagg ttccgccgtc ggctcgggtg
     8761 ttgtctcggc tgtcggctcc ggggcagggg cctcggtggg cacgggttcc ggggtcggct
     8821 cggcagcaga gtccggcggg ggttcggtgg tttccggagc cggttccgcc gtggggtcgg
     8881 gcacttcggt ctcgaccacg acttcttccg ccgcaggcgg gtcagggtca gccggggcag
     8941 ccacggcggg cagctcggtc agggcgggca gatcgtccgc cgcgaacact gtgctggccg
     9001 tgcttgccgc catacagcag gccagaaaca cagccaacag acgttttctc atatgctgtt
     9061 cctcctcaca cagtctccca tgggggttgt cccccacagg gatcgctttg gagtatacag
     9121 cccccgcccc gcaaaatgcg tcgaggtgcg tggatgtgca aaaggttata ttttccagca
     9181 aaaagaaaaa gcacctgcca aaactggcag gtgctctggt aaattatgca gtttttatgc
     9241 ctggtcgtac ttgctgggca cggcgcgcag ggagatcgtg cggtcaaagg tgctgtatgc
     9301 ggtcaggtag gcggtaaagc agccgtccat ccggcccatg ctgccctcct tgccggcggt
     9361 gtagttatgc tcggcaaagc aggggccgac cgaaataccg ttcacgccct tggatgcgac
     9421 cagcgtgccg tccttgacga cgacaccgcc gccgtcattg ccctcgagca tgaaatgctc
     9481 gttctcgacg cggcagcccg cgttgaaggc cagctccacc cgcagcgggg cgcggtcgat
     9541 accggagttg acgatgtgga tgtccaggcc gttttcgacc tcgttcacct gcacgtcaaa
     9601 caccatgtcg gggccgtaga gcttttcgcg gtgggcgttg tccatctgcc accaatcgct
     9661 ggtggcgggc ttttcggcaa agggcaggta ataccagccg gtcatggtct ggtgcagggc
     9721 atagccaccg tccttggggg ccagcgctgc ggggacaaag ttgcggtgct cgcagaagct
     9781 cgcgccgatg cgcacggtca gcgtgaagtc gccgtgctgg aagaacaaaa agctcgggct
     9841 gttgttcaag atcgtgtagg accagcttcc gcggcggcag cgcacaatgc ccgacccttc
     9901 gtaatattta tggtagttcg tcggggtcgc gctgccctcg tgctccagcg cggccagttc
     9961 gggcagcagc atcaggttga tgaggcagtc aaagctgcgc agggcatggc gctccaccat
    10021 ctgcataatg gcgttggcgg cgtccagcag ttcgggcttg cggcaggtat agcccatgta
    10081 gaggtactcg aggtaatact cccgcgggta gatctttttg cccatgtccc agcgggtgga
    10141 gttgttggta aaaatgctgt cgtcggggtc gatgtaggtc agcatcattt ccaggttgcg
    10201 cagcaccggc gcatagtagc tctcgtcgcc ggtggccacg gccagcatga tcatggcatc
    10261 gttgttgatg cggttgtagt tgccggccga acgctcggcg tactcgccgt cctcgttgca
    10321 gtcgttgccc tctttcagaa tggcctcggc ggcggtgcgg cattcgggct tatcaaacag
    10381 cttggcgcag agcatcagca ccgacgcgat ggcccagcgg tggttcgggg tgtggaagcc
    10441 gcagtggcac agggcatcgg ctgcatcgcg gatgatggcc tcatacttag gcttcagctc
    10501 tgcggcagcg gccttggctt cgggcgcggc gggcagtgcg ccgtccacga ccttgcacag
    10561 gtagacatag gcgggcagca gccgcttggt gcagaaagcc gtatccgggc cggagaagaa
    10621 gttgcagttg atgaggtcaa agtaaccgcc gggccgctgg gagcggcgca caaaagccag
    10681 cgccagtgcc aggcggcggc ccagctcggc gtccaggtag taacggctct cggggttcag
    10741 gtagcaggcc gtcattgtcg tggcgcggta gatggccgtt ttcggctcgg ccaggctgtc
    10801 cgccttgggg aaaccgccgc agaaggggct gttctcgtcc aaaatctgga tatcgagata
    10861 gcgctgcacg cggctttcgc tgtcgtgcag gatacgttca tagtgtttgc gcataggtcc
    10921 gctctcctta tttgcctttg cccagcaggg ccgcgccgat gatgcccgcg tcgccgaaca
    10981 gctcggccga catgatgcgg ggctgcttgg ggcagctgcg gccatagccg tgtgcggcat
    11041 tgaacgcccg gatcggctcg accagtacgt cgctgcggcg gctgatgccg ccgcccaggc
    11101 agagcacctc gggctggaag atgttgatga tgctcgtcac gccgacgcag aggtagtgta
    11161 cataggtgtt gaccacctgc acggcggtct cgtcgcccgc gtcgtaggca gccaggatga
    11221 ccttgccatt gacgttggct tcctcgtttt ctgccagcgc ccaaagcttg ctctccttat
    11281 gggcggccat ggcctcccgc gcctggcaga tgagcgccgt gacgctgccg tactgctcaa
    11341 agcagccgcg cataccgcag ttgcactctt tcccgtgcat atcaatcaca aagtggccca
    11401 gttcggccgc tgcgccgttg cagccggtca gcagcttgcc gttcagcact acgccgccgc
    11461 cgacaccggt gcccagcgtg accatgacca tgctgctgct ccctttgcct gcacccgcgc
    11521 agtactcgcc ccaggcggca acgttggcgt cgttatccac atagacgggc aggccggtgc
    11581 gctcgtggat catctggccc agcgggacgt tgtcaaattc cagattgttg gcatacagca
    11641 cggtgccggt gtccacatcg acactgccgg gcgcaccgat gcccacccag gcggcatcac
    11701 tcagggccaa gcccagggca gtcgccatgc ggcggcagag cagcgcgata cggtcggcga
    11761 tgtccgacgc cgggcagggc agcccggtct tgatgctgtc ggtcagcagc agtttcccgt
    11821 ccaggtcggc aatgccgcct gcaatgttgg tgccgcccag atcgattccg atacaataac
    11881 gcataatcta cctcgcttct ttcactttat ttcaatttct gcgtcggttt gcagaatttc
    11941 gtaaacagta taaacacgat gaaaaatgcc agtgcggaca gcggcaggac ccggatgctg
    12001 acggcatagc ccgtcatatc caccagcttg ccgaagccga cgttgaacag gatatccgcc
    12061 agcgacgcga tgcttaaaat cgtgcccgcc gcgccggaga cctgctgcgc cgggaaatag
    12121 agccggatgc tcatgaccag cgtggggtac aaaatggaga aggacaagcc gctgatggcc
    12181 cagacgggca tcgtcaccgc gccgcccagc gagccgatca gatacagcac acaggacacg
    12241 ccgccgaaag ccgccaggct tttgagggcc cccagcttat ccaccagcgg ggacaggcag
    12301 agccgcccga tcatcatacc gccaaagaac accgaaagat acttggccgc cgaggcctgg
    12361 ggcaggccca gaccatctac gccgtaggcg accatccagt tcatgacgcc gtgctcggcg
    12421 ataaagtaga acccgaacac cagcacatag ggcacgatga tacgccagtc atagccgccc
    12481 ttttgtgccg gggtatccgc cggggcggcg gcttcctccg gcacattgca gaaaagcagc
    12541 agcacaaagg ccaccgcgcc caggatcagc agcaccagat ttgtgtgctg ccagaaggca
    12601 aagccatcgg cgtggctgcc caaaatatac tggctgccgc tggtgccgat ttcctgggtg
    12661 aagaacagga agttgacgaa gaaccccggc gctgtggcaa acagcagcgg cgtgatgagg
    12721 ttcatcgtgg tattcaataa ggtggaagaa cccagcgcca gcgccatgcc gatgagcagc
    12781 accgtgtaat tgctggtaaa ggcaaacagc gccagggcgg ccatccaaat caggatcagc
    12841 acctgcagca cgcgcttttt ggaaaagcgg gtgctcaggt tgccgcccac cagcaaaaac
    12901 accaggttgc cgatgtagct tgccgtgacg atcagcccca gctgggtcgt cgtcagggag
    12961 aaatgctctt ggaagatcgt ggaaaaaatt ccgcgcaggc tgtcattgct gccaagcccc
    13021 accatgagca ccacaaaggc aaagaaggta aggcggctgc tccatttttt tgtcatcaga
    13081 cctgggcctc agcttccatc aggaacagaa ttgccatggc ctggccgtag ggcatcgggt
    13141 gcagggggat ctgcttgtag aagtccttgc tctcgcggcc catcggggtg ccgtaggaca
    13201 cctgctgcac aacgcccttt tcatcgatgc agtccaacac aggctgcaag gcgcgcatgg
    13261 cattggcgcg gtagctttcg ggcacgatgc cctgctcgac ggctttcagg atgccgaagc
    13321 cgaagccgct ggtggcgctg gcctccaggt aggaggtcgg gtcgtcgatc agggtgtgcc
    13381 acataccgcc ctcgtcctgg tacttggcca ggctctcgat ctggcgcagc agcgcgttct
    13441 caaggatgcg gcggtcgggg ttgcctacgg ggacgatttc cagcagcagg gggatggcag
    13501 ccgtcaccca gctgttgccg cggccccaga aagcctcggc aaagttatgg cagccattaa
    13561 agcagaaacc atgataccac agaccggtct ttttgtcagc cagatatttg gtgtggacaa
    13621 ggtactggta gacagcctcg tcaatgtagt cctggcggcc cagaatgcgg ccgatattgg
    13681 ccagcggcag cacagtcatg aacagcgtat cgtcccacag ctcgccatcg ttgacgcgat
    13741 cggatgtgac atgctggaag ccgccctcgc ccgcgcgggg caggccgtgc atggcccact
    13801 cggcccactc tttgcagata tcgccgtact tttcattctt ggtgtactca tacaggtagc
    13861 tcagggcaaa aatgggggcc atcgtgttga tgttcttgct gggcaggccc tcggcaatgc
    13921 gggcgtcgta gtactctgtc agcacatcca ggcagcgctg gtcgtgcttt ttctcaaaca
    13981 gcttccacag gccgtacagg cccacgccct gagtccactc ccacagcata tagcgggcca
    14041 gattgccctg atccatgttg gattttgcca tgttctccag gaaagtctcg tccctttcat
    14101 acaggacaga tttgaagctc tcaatgcaaa gctccagttt gttttcaata tcagctttct
    14161 gcatggtggt tttctccttt ttattataat aggtagacat aacgttctaa cttgcctaaa
    14221 gtatagcata gggacaaagg caaaaaccac tctgattttg tctttatcgc tgaaatatat
    14281 tgcacttttt gcgagtgcga gtataataga gacaggaaca tttttgacgg aggctgccta
    14341 tgtatctgga cgacgcattg ctgtacaatc aggggtacat gatttcgatc aacgacctgg
    14401 attcgccgct gttttacctg ttcgactacg attcgcggtc gttcaagatc aacatgagtt
    14461 tccagcattt ccaccagttt cacgagatac acattctgtt aagcggccgt gcatcacatc
    14521 tcatcgaggg cgactattac accctgcagc ccaagtacat gaccggtctg ctcgacatca
    14581 acgccggccg caaggccgcc cgcttcgtgc gcttctgcga tgccttcaac
//
LOCUS       DBMDSQ010000021        14568 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_1300_length_14568_cov_2.469344, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000021 DBMDSQ010000000
VERSION     DBMDSQ010000021.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 14568)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 14568)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14568
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1300_length_14568_cov_2.469344"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1497)
                     /locus_tag="ACZPAX_01580"
     CDS             complement(<1..1497)
                     /locus_tag="ACZPAX_01580"
                     /inference="COORDINATES: protein motif:HMM:NF013357.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH25 family lysozyme"
                     /protein_id="HIR1112621.1"
                     /translation="MPEERKVYRSPARAAAKARPAKTAAPRPPQPPKKPKRRSAKRRR
                     SMLVLGLCLLCLVVVLVVSVVLVRCTAEPEGPAAPDFGTPADAWQKNELGYYFNTSGQ
                     AMPAAVLKGMDVSKFQGEVDWEKAKAAGIDFAIIRCGYGGEWDGQEENWAQDDTYWRR
                     NADECTRLGIPFGTYLYSYATTVEEARSEADHVARLLGLTAPPQEGLDDYTAAPYQLS
                     YPVYYDLEDKYISGVFPAEMAELTEAFFSRLEEHGYTGKQGLYASLNWVRGRFSDPGF
                     DQWRDNLWIARFADELGYNGTYDMWQSTYSAPGADYGVQSETVDLDFVMRPFTFTGVS
                     ACNGKTAAPVLLNDTRTDELHMDGKDAYATLATNEPGEDEGGRRVYWATSDKNIATVD
                     KNGTVRARTDSGECTITATLADGTESISCLVRIGDITVPVFATAGLHGDRTTLADAAA
                     LKASTPDSILLDAGGALHGTQSASLTGGMDMLSGFSAAGYDLQAMALDS"
     gene            complement(1791..3110)
                     /locus_tag="ACZPAX_01585"
     CDS             complement(1791..3110)
                     /locus_tag="ACZPAX_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012058731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1112622.1"
                     /translation="MTKGAPLGHLFLYAVPLLLGNWLQLAYNAVDSIIAGRFIGQDAL
                     AAEGVAGPVMNLVILAISGLCIGAGVLMSEAFGAKRTDRLQETLATTLLFGAVLCCGA
                     AALGCVLTPWILRALAVPDAIFGITAIYLRITFLGAPFTFFYNALAAGLKSVGDSKTP
                     LKFLAFSAVLNAVLDLILIGGLGFGIVCSAVTTVAAEAASAVLAAVYMARRVPELCPG
                     RGQWRIRRDLLGPILRYGSVTALQQAVQPICKVLIQGQVNALGVEAIAAFNAVTRVDD
                     FAFTPEQSIATAITTYIAQNRGAQQNERIRRGFAVGLRLELGYWLLVGCVTLAFRGPI
                     LSLFVAGEGAADVVALGSEYLGHMAVFYALPALTNGFQGFYRGMGKMTTTLIGTCMQA
                     GLRAAAAAVLAPRVGLQGIAFACAFGWCVMLAFEVPYYFWTCKKKGL"
     gene            complement(3132..4697)
                     /locus_tag="ACZPAX_01590"
     CDS             complement(3132..4697)
                     /locus_tag="ACZPAX_01590"
                     /inference="COORDINATES: protein motif:HMM:NF025622.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase domain-containing protein"
                     /protein_id="HIR1112623.1"
                     /translation="MDWQKKVSLWCAKNFRPLVVALLVGCGVHYTIYANQLGTMDAVH
                     IGALYIADGWPEHLYWETEQGRWALRLVDMLRGGINQPALSALLMLFLYALAGVLLTD
                     LFGVRSRTAKYLIPLTLVCAPYVAEIEFFHYCSVSYALSFLLAVLAVQSAVCGVRFAW
                     LAGTVCMAFSLGMYQANLGTAAGLCVMLLLLAVLRRPGAWKATGLTALRMVLMGGSGA
                     VLYMLILKVFLRLYDVGLSGVNGINAVGLDTLRSLPLGLKNAYFDFYAYFFTHGIAQN
                     HYGQIAGYLLLFVLAALAGLRWLIVLHDRKAAAAAVVLVALLPAAANVTDVINTGATV
                     ALRMAGSLAIVVPFAIAVIDTAPALTERAFSWGMALCTAFCAVLLRGFAVQVNNDAAV
                     MLKQKTTVVNLANRVCTRLEENADYQNGAEVVILGEPKRGAYPEESPLKPMAGELAQF
                     GPLSYDPTFNAHGWYVLVWDELGVQLNECADETVRAISNSDAFKAMPNYPADGCIQTI
                     DGVVTLKVADFPF"
     gene            complement(4701..5645)
                     /locus_tag="ACZPAX_01595"
     CDS             complement(4701..5645)
                     /locus_tag="ACZPAX_01595"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013977129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR1112624.1"
                     /translation="MLISLIVPCYNEEEAMPLFYKEASRVAAEMKTSHGADFEFIFVD
                     DGSRDGTLRVARELHAQDDRVRYVSFSRNFGKEAGIYAGLQAARGDYVATMDADLQDP
                     PALLPQMLDTLLTGEYDCAATRRTTRKGEPPLRSWFARKFYQIINKMSDTEIVDGARD
                     FRLMSRKMTNAVLSMAEYNRFSKGIFSWVGFKTKWFDYENIERVAGTTKWNFWGLFKY
                     SIEGIVGFSTTPLLMAAGVGVLFCLLAFIGIIFVIVRALMFGDPTSGWPSLVCIILLC
                     SGVQLFCTGIVGEYLAKTYLEVKHRPIYIVAETEEDKK"
     gene            complement(5905..8448)
                     /gene="leuS"
                     /locus_tag="ACZPAX_01600"
     CDS             complement(5905..8448)
                     /gene="leuS"
                     /locus_tag="ACZPAX_01600"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005944342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HIR1112625.1"
                     /translation="MKYDFKTVEAKWQKVWEDEDTFHAEIDHSKPKFYALVEFPYPSA
                     AGLHVGHPRSYTALDIVARKKRQCGYNVLYPMGWDAFGLPTENYALKNHINPEIVTAQ
                     NVARFKSQLKALGLSFDWDREINTTDPEYYKWTQWIFIQLFKKGLAYKKETTVNYCTG
                     CKVVLANEEVVNGVCERCGSPVVQKNKSQWMLKITAYADRLINDLDDVDYIDRVKTQQ
                     RNWIGRSHGAEINFDTTAGDTLTVYTTRADTLFGATYMVISPEHAFIKKWVDAGLIKN
                     ADAVAAYQEEAARKSDFERTELNKEKTGVVIEGVKGINPVNGKEIPIFISDYVLATYG
                     TGAIMAVPAHDTRDWEFAKKFGLPIIEVVKGTVPSDLDKEAFTDVATGTLVNSDFLNG
                     LSVEDAKNKMYAWLEENGKGHKQVNFKLRDWVFSRQRYWGEPIPMVYCDKCGWVPLPE
                     SELPLRLPEIKDFEPGENGESPLARHTEWVSTTCPCCGAPAKRETDTMPQWAGSSWYF
                     LRYCDPKNHDAIASKEALEYWSPVDWYNGGMEHTTLHLLYSRFWHKFLYDIGVVPTKE
                     PYQKRTSHGMILGLNPHAFENQPDAERKRLLAEYGDENGARKALVEKYGEMAEHPIVK
                     MSKSLGNVVNPDDVVNEYGADTLRLYEMFIGDFEKAAPWNTSSIKGCKRFLDKIWSMS
                     EKLVPGEGVRPALEAVANRTIKKVGEDIDALKANTAIAQLMIYVNALSDNGGATKAEY
                     ELLLQLLNPFAPHMTEELWQQLGHTEQLAYHAWPTYDEAKCVEQTIEIAVQVNGKVKA
                     RIKVAAAIESADAIAAAKDEPAVAEAIAGKTIAKEIYVKGKLVNIAVKG"
     gene            complement(8717..9091)
                     /gene="rsfS"
                     /locus_tag="ACZPAX_01605"
     CDS             complement(8717..9091)
                     /gene="rsfS"
                     /locus_tag="ACZPAX_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015536738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HIR1112626.1"
                     /translation="MESKELAIRLARALDAKKAVNVHILAVEDLTTVTEYFVIATGMS
                     TTHVGALADEAEFQLDREGVKVLRTEGHDGKRWVLLDYGSVIVHVFTQEAHDYYDLEH
                     LWADATVVPASEWMPDEGADEQ"
     gene            complement(9113..11044)
                     /locus_tag="ACZPAX_01610"
     CDS             complement(9113..11044)
                     /locus_tag="ACZPAX_01610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HIR1112627.1"
                     /translation="MSEPRKLYTAGATGSSEQEQYEAARRRAQQLAEQNARRRTVQQP
                     MRTAQGRHISQRPAARPEPEPIREPIQEPVQQPVQQAAPQPDARRELTYAQRQAMAYR
                     AAAERKYGDTLQFQVQRPRRTVNRPQAAQTAAPQQAQQAANRLYDQPAAASRQPAAQR
                     PRAQVQKMDLRAAQGSKPMYDIEAAGGIQLEDAAPRRAKRAGAPKTAAGGNGGRKPPR
                     DTGDAPRKSKGKKSGKKGGDGKGGNGKKKKKIRWWQILLITLLVIALIFGGAFALIMG
                     SIAPAGGSIKLNQLINTPKEFQGKELNFLMVGIDRSSQKEGDDLNDAGVDDGNTDMIL
                     YVHFNNETGEMKMLQIPRDTMVTTDRSVSGNYRINNIAKTQAADSNSMNMAALCEQIS
                     NMYQLPIDGYMTIRLEMLVQLVDSFGGIELYVPQEMDYKGSHLDQGYQTLNGDACEFL
                     LRTRHIYPDGDIGRLNMQRQFYSALFRRLKSIGNIWDVAKLTPAVLNYMETSLSASQL
                     VSFAVSMLKIDSSKIMICQMPIKMAGKYNGQAVVYPARQEDADILNQYFRENTGFVDA
                     SELQLCDDASVLGFDLSGYTATDANVQYMGQLNQEAADAQQNQNLDGSHTVEYVDSES
                     TDTADSSSDSTESESQPAA"
     gene            complement(11075..11641)
                     /gene="yqeK"
                     /locus_tag="ACZPAX_01615"
     CDS             complement(11075..11641)
                     /gene="yqeK"
                     /locus_tag="ACZPAX_01615"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005932887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HIR1112628.1"
                     /translation="MDYDEAKKLVKSRLGEKRWTHTKNVKKMAVKLAKRWGADPEKAA
                     MAAILHDSAKELPKQELLQIFADNAIIAENAPARPSPVWHGIAAAILAQTQWGITDPE
                     ILSAIRCHTTGKADMSLLDKIIYLADMTSAERDWPGVDELRKLEMQDLDRALCDALKR
                     SIDFVEEKSGTLDTESVAAYEYAKARLK"
     gene            complement(11767..12366)
                     /gene="nadD"
                     /locus_tag="ACZPAX_01620"
     CDS             complement(11767..12366)
                     /gene="nadD"
                     /locus_tag="ACZPAX_01620"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015536736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HIR1112629.1"
                     /translation="MKILLYGGTFDPPHNGHKNNLRAALERVRPDRAIVMPAGTPPHK
                     HASATPAELRLQMCQCFRALSPAVEVSDWEIRQGGRSYTVHTLEMLRGANPGAELYLC
                     VGSDMLLTFGEWYRWQDMLKMAALVVESRTGTDDAALHAAAAKLEQAGGTVLFAQAES
                     YPCASSDIRSGKIPPEKWPEVLPPEVLAVIRQNRLYGSL"
     gene            complement(12363..12665)
                     /locus_tag="ACZPAX_01625"
     CDS             complement(12363..12665)
                     /locus_tag="ACZPAX_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YhbY family RNA-binding protein"
                     /protein_id="HIR1112630.1"
                     /translation="MSLTSKQRAALRGAANTMDPVYQIGKGEIDETLIQGIADCIAAR
                     ELIKVKVLENSEYDPRQAADILCEAVGCECVQVIGRKFVLFKQKKKESAYAAVLAK"
     gene            complement(12811..13440)
                     /gene="rdgB"
                     /locus_tag="ACZPAX_01630"
     CDS             complement(12811..13440)
                     /gene="rdgB"
                     /locus_tag="ACZPAX_01630"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005932884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035870 - dITP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0036222 - XTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HIR1112631.1"
                     /translation="MIVCAASNNAGKLKELRRILERMGHEVKSLRELGIALDPEETGT
                     TFAENARIKAEAFCKASGLPTVADDSGLCTDALDGAPGVYSARYAGHHGDDDANNAKL
                     LQEMADVPAEQRGAKFVSAVCFMLPDGRALEVEGECPGRIAFSLQNGDYGFGYDPLFI
                     PDEYGAPDGKHPNAEARSYAQLTPDEKDAISHRGRAMEKLEEKLPAFLK"
     gene            complement(13437..14435)
                     /gene="ftsY"
                     /locus_tag="ACZPAX_01635"
     CDS             complement(13437..14435)
                     /gene="ftsY"
                     /locus_tag="ACZPAX_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006523622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HIR1112632.1"
                     /translation="MGFFNFFKRDISEEEFQEQQKVKRGLEKTRTGFFQNIVNTLTHA
                     QINDDLYDQLEEQLILADVGPACAVRLVDELHDEVEEKHLKTGQEALDTLRGIICQEL
                     TPRYPMELDGKPAVILVIGVNGVGKTTTIAKLAALYKKQGRRVMLAAGDTFRAAASEQ
                     LELWADRAGVPLVQAGMGADPAAVIFDAVKSANARGYDMVIADTAGRLHNKKGLMDEL
                     SKISRSVKKASPEASLEVLLVLDAITGQNAISQAEEFCKAASATGVVLTKLDGTPRGG
                     CAVSVWENLGLPIRFIGVGEKIDDLMEFDAQTFVESLLPESALQKEEQKEEGEGEA"
BASE COUNT         2558 a         4526 c         4487 g         2997 t
ORIGIN      
        1 gctgtccagc gccatggcct gcaggtcgta cccggctgcc gagaagccgg acagcatatc
       61 cataccgccg gtcaggctgg cgctctgggt accgtgcagc gccccgcccg catccagcaa
      121 aatcgaatcc ggggtcgagg ccttcagcgc ggcggcgtcc gccagcgtcg tgcggtcgcc
      181 atgcagccct gctgtggcaa agaccgggac cgtaatgtcc ccgatgcgca ccaggcagga
      241 aatgctctcg gtaccgtcgg ccagcgtggc cgtgatggta cactcgccgc tgtcggtgcg
      301 ggcgcggacc gtgccgtttt tgtccacggt ggcaatgttt ttgtcactcg tcgcccagta
      361 gaccctgcgg ccgccttcgt cctcgcccgg ctcgttcgtc gccagcgtgg cataggcgtc
      421 cttgccatcc atgtgcagct catcggtgcg ggtgtcgttc agcagcaccg gcgctgctgt
      481 cttgccgttg caggccgaca cgcctgtaaa tgtaaacggc cgcatgacga aatccaggtc
      541 caccgtctcg ctctgcacac cgtagtccgc gccaggggcg ctgtacgtcg actgccacat
      601 atcgtaggtg ccgttgtagc ccagctcgtc ggcaaagcgg gcgatccaga gattgtcgcg
      661 ccattggtcg aagccgggat ctgaaaagcg gccccgcacc cagttcagcg aggcataaag
      721 cccctgcttg ccggtgtagc cgtgctcttc cagccgggag aagaatgcct cggtcagttc
      781 ggccatctcg gcggggaaaa caccgctgat gtacttatct tccaggtcgt agtagaccgg
      841 gtagctcagc tggtagggcg cggcggtgta gtcgtccaga ccttcctgcg gcggggcggt
      901 caggcccagc agccgggcaa cgtggtcggc ctcactgcgg gcctcctcca ccgtcgtcgc
      961 gtaggagtag agataggtgc cgaaggggat gcccagccgg gtacactcgt cagcgttgcg
     1021 ccgccagtag gtgtcgtcct gggcccagtt ttcctcctgg ccgtcccact cgccgccgta
     1081 accgcagcgg atgatggcaa agtcgatgcc ggcggccttg gccttttccc agtccacctc
     1141 gccctggaac ttggagacgt ccatgccctt gagcacggct gcgggcatgg cctggccgct
     1201 ggtgttgaag tagtagccca gctcgttttt ctgccaggcg tcggcgggcg tgccaaagtc
     1261 cggcgcggcg gggccttccg gctcggccgt gcagcgcacc agcaccacag acaccaccag
     1321 caccacaacc aggcagagca aacacagccc cagtaccagc atactgcggc ggcgtttggc
     1381 actgcggcgc tttggctttt tgggcggctg cggcgggcgc ggggccgccg ttttggccgg
     1441 tctggctttg gcggcagcgc gggcggggct gcggtagact ttgcgttctt ctggcacggc
     1501 ggtttacctc tctccgggta gattttatgc agggatagct attattagtg tagcattttg
     1561 tcgaagaaat tacaatcagc cgcagcagat attggggaaa ttttacacaa aaaccccgtc
     1621 gtggatacgg cggggtcgta ggggggcggc gtcctcgacg ccccgcaaat cagtagatgt
     1681 tacacggggt caaacgctgt tcgtccgcgc cacaaaaggc aaacggccgc ccgcctttca
     1741 cgcgcgggcc gggcatgccc ggcccctaca ggagctgcac tgccgagact tcacagcccc
     1801 ttctttttac acgtccaaaa ataatacggc acctcaaacg ccagcatgac gcaccagccg
     1861 aaggcgcagg caaaggcgat gccctgcagc cccacacgcg gggccagcac agcggccgcg
     1921 gcggcgcgca gacccgcctg catacaggtc ccgatcagcg tcgtcgtcat cttgcccatg
     1981 ccgcggtaaa atccctggaa accgttcgtc agcgcgggca gagcatagaa caccgccatg
     2041 tgccccaggt actcgctgcc cagggccacc acgtcggcgg ccccctcccc cgccacaaac
     2101 agcgagagga tcggcccgcg gaatgccagc gtcacgcagc ccaccagcag ccagtaaccc
     2161 agttccagcc gcaggcccac ggcaaaaccg cggcggatgc gctcgttctg ctgtgcgccc
     2221 cggttctggg caatgtaggt cgtaatagcc gtcgcgatgc tctgctccgg cgtaaaggcg
     2281 aaatcgtcca cccgcgtgac cgcgttgaac gccgcaatgg cttcgacgcc cagcgcgttg
     2341 acctgcccct ggatcagcac cttgcaaatc ggctgcaccg cctgctgcaa agccgtgacc
     2401 gagccatagc gcaggatcgg ccccagcagg tcgcggcgga tgcgccactg cccgcgccca
     2461 gggcaaagct ccggcacgcg gcgggccatg taaacggctg ccagcacggc gctggcagcc
     2521 tcggccgcca cggtggtgac ggcgctgcac acgatgccga agcccagccc acctatcaga
     2581 atcaggtcga gcacggcgtt caacacggcg gagaacgcta aaaatttcag cggcgttttg
     2641 ctgtcgccga cgcttttcag ccccgccgcc agcgcgttat agaaaaacgt gaacggtgcg
     2701 cccaaaaacg tgatgcgcag atagatggct gtgatgccga aaatcgcatc cggcacggcc
     2761 agtgcgcgga gtatccacgg cgtcagtaca cagcccagcg ccgccgcacc gcagcagagc
     2821 accgccccga acagcagcgt cgtcgccagc gtttcttgca gccggtcggt tcgtttggcc
     2881 ccgaacgcct cactcatcag cacgcccgcg ccgatacaca gcccgctgat ggccaaaatt
     2941 acaaggttca tgaccggccc ggccacgccc tcggcggcca gggcatcctg cccgatgaac
     3001 cgccccgcga tgatggaatc caccgcattg tacgccagct gcagccagtt ccccagcagc
     3061 agcggcaccg catacagaaa gaggtgcccc agcggggcac ccttggtcat atcacgcatc
     3121 attatcagct ctcaaaacgg aaaatctgcc accttcagcg tgacaacgcc gtcgatggtc
     3181 tggatacagc catctgccgg gtagttgggc atggccttga acgcgtcgga gttgctgatg
     3241 gctcggacag tctcgtccgc gcattcgttg agctggacgc ccagctcgtc ccacaccagc
     3301 acataccagc cgtgggcgtt gaacgtcggg tcgtaggaga gcgggccgaa ctgcgccaat
     3361 tccccggcca taggcttgag cggggattcc tccgggtacg cgccgcgctt cggctcgccc
     3421 aggatcacga cctcggcgcc gttctggtag tcggcgtttt cttccagccg ggtacacacg
     3481 cggttggcga ggttcacaac ggtggtcttt tgtttcagca tgaccgccgc gtcgttgttg
     3541 acctgcacgg caaacccacg cagcagcacc gcacagaacg ccgtgcagag cgccatcccc
     3601 caggagaacg cccgttccgt caacgcggga gccgtgtcga tcacggcgat ggcaaacggc
     3661 accacgatgg ccagcgagcc cgccatccgc agcgccacag tggccccggt gttgataacg
     3721 tcggtcacat tggctgcggc gggcagcagg gccaccagca ccaccgccgc tgcggcggcc
     3781 ttacggtcat gcagcacgat gagccaccgc agcccggcca gtgccgccag cacaaacagc
     3841 agcaggtagc ctgcgatctg cccatagtgg ttttgggcaa tgccgtgggt gaagaagtaa
     3901 gcgtaaaaat caaagtaggc gtttttcagg cccagcggca gactgcgcag cgtgtccagc
     3961 cccacagcgt tgatgccgtt cacaccggac aggcccacat cgtacagccg caggaacacc
     4021 tttaaaatca gcatatacag caccgcgccg ctgccgccca tcagcaccat gcgtagagca
     4081 gtcaggccgg tggctttcca cgcgccgggg cggcgcagaa ccgccagaag cagcagcatg
     4141 acgcacaatc ctgccgccgt acccaggttg gcctggtaca tacccagcga gaacgccata
     4201 cagaccgtgc ccgccagcca ggcaaagcgt acaccgcaga cagcggactg caccgccagc
     4261 acagccagca ggaaggacag cgcataggac acgctgcaat agtggaaaaa ctcgatctcc
     4321 gccacatagg gcgcacagac cagcgtgagg ggaatcagat attttgccgt gcggctgcgc
     4381 acgccgaaca aatccgtcag cagcacaccc gccagcgcgt agaggaacag catcagcaat
     4441 gcagaaagtg caggctggtt gatgccgccg cgcagcatat ccaccaggcg cagtgcccaa
     4501 cggccctgct cggtttccca atacaggtgc tcgggccagc cgtcggcaat gtacagtgcg
     4561 ccgatgtgta cggcatccat cgtgcccagc tggttagcgt agatcgtgta atgtacgccg
     4621 cagccgacca gcagcgccac gaccagcggg cggaagtttt tggcacacca caggcttact
     4681 tttttctgcc agtccatcgt ttatttttta tcctcttcgg tctcggccac aatgtagatg
     4741 gggcggtgct tgacctctaa gtaggttttg gccaggtact cgcccacgat gccggtgcag
     4801 aacagctgga cgccgctgca cagcagaatg atgcagacaa ggcttggcca gccggaggtc
     4861 gggtcgccga acatcagcgc ccggacgatg acaaaaataa tgccgatgaa cgccagcagg
     4921 cagaacagaa cgcccacgcc cgccgccatc agcagcggcg tggtcgagaa gccgacaata
     4981 ccctcgatgg agtatttgaa caggccccag aaattccact tggtcgtgcc ggccacgcgc
     5041 tcaatgttct cgtagtcgaa ccatttggtc ttaaagccga cccagctgaa aatgcctttg
     5101 ctgaaccggt tatactcggc catgctcagc acggcattgg tcatcttgcg gctcatcagg
     5161 cggaaatccc gcgcaccatc gacaatctct gtatcagaca tcttgttgat gatctggtag
     5221 aatttgcggg cgaaccagct gcgcagcggc ggctcgccct tgcgggtcgt acggcgggtg
     5281 gcggcgcagt cgtattcgcc ggtcagcagc gtgtccagca tctgcggcag cagcgcaggc
     5341 gggtcctgca ggtcggcgtc catcgtggcg acgtaatcac ccctggcggc ctgcagaccg
     5401 gcgtaaatgc cggcctcctt gccaaagttg cggctgaacg agacatagcg cacccgatcg
     5461 tcctgagcgt gcagttcgcg ggccacacgc agcgtaccgt cgcgggagcc gtcatccaca
     5521 aagatgaact caaaatccgc gccgtggctg gttttcattt ccgccgccac gcgggaggct
     5581 tctttataaa aaagcggcat cgcttcttct tcattgtaac agggtacgat caaactgatg
     5641 agcatggtgt gacctgcttt cgtcaaaata ttgtatcttt tattatagcg tagtttggcg
     5701 ggtttgtaaa caatggatgt ggcggggttc ctaaagcctt ggccccacag ggccggatga
     5761 ggggcgcgtg tgccgcagcc gcccagtagc gggttgtaag gcaaggccgc ctctcatcag
     5821 tcagcggtcg gggccgctga ctgaatggct tctgcaacac aaaaaaggca gagctttcgc
     5881 tctgccttgg gttcgtgcta aaaatcagcc tttgaccgcg atattgacca gcttgccctt
     5941 gacgtagatt tccttggcaa tggtcttgcc ggcgatggct tcggccacgg cgggctcgtc
     6001 cttggcggca gcaatggcgt ccgcgctctc gatggcggcg gccaccttga tgcgcgcctt
     6061 gaccttgcca ttgacctgca cggcaatttc aatcgtctgc tccacgcact tggcctcgtc
     6121 gtaggtgggc caggcgtggt aggccagctg ctcggtgtgg cccagctgct gccacagctc
     6181 ctcggtcatg tggggggcaa acgggttcag cagctgcaac agcagctcgt actcggcctt
     6241 ggtcgcacca ccgttatcgc tcagcgcgtt gacatagatc atcagctggg cgatggcggt
     6301 gttggccttc agtgcgtcga tatcctcgcc gaccttcttg atggtgcggt tggcaacggc
     6361 ctccagcgca gggcggacgc cctcgccggg aacgagcttc tcgctcatgg accagatctt
     6421 atccaggaag cgcttgcagc ccttgatgga ggaggtgttc cacggagcgg ccttctcgaa
     6481 gtcgccgatg aacatctcat acaggcgcag ggtgtcggca ccgtactcgt tgacaacatc
     6541 gtcggggttg acgacgttgc ccagggactt ggacatcttg acgatcgggt gctcggccat
     6601 ctcgccgtat ttctcgacca gggccttgcg tgcgccattc tcgtcgccgt actcggccag
     6661 caggcgcttg cgctcggcgt cgggctggtt ctcaaacgca tgggggttca ggcccagaat
     6721 catgccgtga gaggtacgct tctggtacgg ctccttggtg gggaccacgc cgatatcata
     6781 caagaattta tgccagaaac ggctgtacag caggtgcagg gtggtgtgct ccataccgcc
     6841 gttgtaccag tccaccgggc tccaatactc caaagcctct ttgctggcga tggcgtcgtg
     6901 gttcttcggg tcgcagtagc gcaggaagta ccagctggaa ccggcccact ggggcatggt
     6961 gtcggtctcg cgcttggcgg gggcaccgca gcaggggcag gtcgtgctga cccactcggt
     7021 gtggcgggcc agcgggcttt cgccgttctc gcccggctca aagtccttga tctcgggcag
     7081 gcgcaggggc agctcgctct cgggcagggg cacccagccg cacttgtcgc agtagaccat
     7141 ggggataggc tcgccccagt agcgctggcg gctgaacacc cagtcgcgca gcttgaagtt
     7201 gacctgcttg tggcccttgc cgttctcctc gagccaggcg tacatcttgt tcttggcatc
     7261 ctcaacagac aggccgttca ggaagtcgga gttgacgagc gtgccggtgg ccacatcggt
     7321 gaaggcctcc ttgtcgaggt cgctgggcac agtgcccttg acgacctcga tgatgggcag
     7381 gccgaacttt ttggcaaact cccagtcgcg ggtgtcgtgg gcgggcacgg ccatgatggc
     7441 gccggtgccg taggttgcca ggacgtagtc ggagataaag atggggatct ccttgccgtt
     7501 gacagggttg atgcccttga cgccctcgat gacgacaccg gtcttttcct tgttcagctc
     7561 ggtgcgctcg aagtcggact tgcgggcggc ttcctcctgg taggcagcaa cagcgtcggc
     7621 gttcttgatg agacccgcat cgacccactt tttgatgaaa gcgtgctcgg ggctgatgac
     7681 catgtaggtc gcaccgaaca gcgtatccgc gcgggtggtg tagacggtca gggtatcacc
     7741 ggcggtggtg tcgaagttga tctcggcgcc gtggctgcgg ccgatccagt tgcgctgctg
     7801 agtcttgacg cggtcaatgt aatccacatc gtccaggtcg ttgatgaggc ggtcagcgta
     7861 ggcggtgatt ttcagcatcc actggctctt gttcttctgc acaacggggc tgccgcagcg
     7921 ctcgcagacg ccgttgacaa cttcctcgtt ggccagaacg accttgcagc cggtgcagta
     7981 gttgacggtc gtttccttct tataagccag accctttttg aacagctgaa tgaagatcca
     8041 ctgcgtccac ttataatact cggggtcggt ggtgttgatt tcacggtccc agtcgaagga
     8101 caggcccagg gctttcagct ggctcttgaa gcgggccacg ttctgggcgg tgacgatctc
     8161 ggggttgata tggtttttca gggcgtagtt ttcagtgggc agaccgaaag cgtcccagcc
     8221 catggggtac aggacgttat agccgcactg gcgctttttg cgcgcgacga tatccagcgc
     8281 ggtgtagctg cgcggatggc cgacgtgcag accggcggca gaaggatacg ggaactcgac
     8341 cagagcatag aacttcggct tggaatggtc gatttcagcg tggaaggtat cctcgtcctc
     8401 ccacaccttc tgccatttgg cctcaacggt tttaaagtcg tacttcataa atttctcccc
     8461 acagataaat ttttgatggg tcgtaacctt cttaaagcct tcccccgagg ggctgcgccc
     8521 gcaggcgcgt gtcggagcgc aaccgccgaa ggcggctctt agcgcgtaga ctgaaggtgg
     8581 cgcgcttgtg cgccgaatga ggggtgggtt tgccgcagtt gcccgtttcc aggtcggctg
     8641 ccgcagggct gcccctcatc agtcagcggt cggggccgct gacagcttcc cccgaggagg
     8701 aagccgttcc gcatttttac tgctcgtccg caccttcgtc cggcatccac tcgctggcgg
     8761 gcacgaccgt ggcgtcggcc cagaggtgct cgaggtcgta gtagtcgtgg gcctcctgcg
     8821 taaagacatg gacgatgacg ctgccgtagt ccagcagcac ccagcgcttg ccgtcgtggc
     8881 cctcggtgcg cagcaccttg acgccctcgc ggtccagctg gaactcggct tcatcggcca
     8941 gggcgcccac atgggtggtg gacataccgg tggcaatgac aaaatactcg gtaacggtgg
     9001 tcaggtcctc aacagccagg atgtggacgt tgacggcctt cttcgcgtcc agcgcacgcg
     9061 ccagacgaat agcaagttct ttgctttcca tagtgtaaac tcctagtgta aattacgcgg
     9121 cgggctggct ttcgctctcg gtggaatcgc tgctgctgtc cgcggtgtcg gtggactcgg
     9181 aatcaacata ctccaccgtg tgcgagccgt ccaggttctg gttctgctgg gcgtctgcgg
     9241 cctcctggtt cagctggccc atatactgca cgtttgcgtc ggttgccgtg tagccgctca
     9301 aatcaaagcc cagcacagac gcatcgtcgc agagctgcag ctcgctggca tccacaaagc
     9361 cggtattctc gcggaaatac tggttcagaa tgtcggcgtc ctcctggcgg gccgggtaga
     9421 caacggcctg tccgttgtac tttccggcca tcttaatggg catctggcag atcatgatct
     9481 tgctggagtc gatcttcagc atactgaccg caaagctgac aagctgcgag gcgctcagac
     9541 tagtctccat atagttcagc acagcagggg tcagcttggc gacatcccag atgttgccga
     9601 tgctcttcag gcggcggaac aatgccgaat agaactgccg ctgcatgttc aggcggccaa
     9661 tgtcgccgtc cgggtaaatg tggcgggtgc gcagcaggaa ctcgcaggca tcgccgttca
     9721 gcgtctggta gccctgatcc aggtggctgc ctttataatc catctcctgc gggacgtaca
     9781 gctcgatgcc gccgaacgaa tcgaccagct gcaccagcat ttccagacgg atggtcatat
     9841 agccatcgat cggcagctgg tacatattcg aaatctgctc acacagggcg gccatgttca
     9901 tgctgttgct gtccgcggcc tgcgtcttag cgatgttatt gatgcggtag ttgcccgaca
     9961 cgctgcggtc ggtcgtgacc atcgtgtcgc gggggatctg cagcatcttc atctcgcccg
    10021 tctcgttgtt gaaatggacg tagaggatca tatcggtgtt gccgtcatcg acgcctgcat
    10081 cattgaggtc gtcgccctcc ttctgggacg agcggtcgat gccgaccatc aggaagttca
    10141 gctccttgcc ctggaattcc ttcggcgtgt tgatgagctg attcagcttg atgctgccgc
    10201 ctgccggggc gatcgatccc ataataagcg cgaacgcgcc gccgaagatc agcgcaatga
    10261 ccagcagcgt gatgagcagg atctgccacc agcggatctt tttcttcttt ttgccattgc
    10321 cgcccttgcc gtcgccgcct tttttgccgg actttttccc tttggacttg cgcggggcgt
    10381 cgccggtatc acgcgggggc ttgcggccgc cattgccgcc tgcggcggtt ttgggcgcac
    10441 cggcgcgttt ggcgcggcgc ggtgcggcgt cttccagctg gatgccgcct gcggcctcga
    10501 tgtcatacat cggcttgctg ccctgggcgg cgcgcaggtc cattttctgc acttgggcgc
    10561 gggggcgctg agcggcgggc tgccgcgagg ctgccgcagg ctggtcgtac aggcggtttg
    10621 cggcctgctg tgcctgctgc ggggccgccg tctgggctgc ctgcgggcgg ttcacggtac
    10681 ggcgggggcg ctgcacctgg aactgcaagg tatcgccgta tttgcgctca gctgcggcgc
    10741 ggtaggccat ggcctggcgc tgggcatagg tcagctcacg ccgggcgtca ggctggggcg
    10801 cggcttgctg gacaggctgc tgcaccggct cctgtatggg ttcccggatc ggttccggct
    10861 cgggccgggc cgccgggcgc tggctgatgt gccgcccctg ggccgtgcgc atgggctgct
    10921 gcaccgtgcg gcggcgggca ttctgttcgg ccaactgctg ggcgcggcgg cgggcagcct
    10981 cgtactgctc ctgctcggag cttccggtcg cgccggcggt atacagctta cgcggttcgc
    11041 tcattgtttt tcctccctgc tgtatggagt gtttttactt tagccgtgct tttgcatact
    11101 cgtaagcggc tacgctttca gtgtccagcg tgccgctctt ttcttccaca aaatcgatgc
    11161 tccgttttaa cgcgtcgcaa agggcccggt ccaggtcctg catttccagt ttgcgcagtt
    11221 catccacgcc gggccagtcg cgctcggcgc tggtcatatc ggccaagtag atgatcttat
    11281 cgagcaggga catatccgct ttgccggtgg tatggcaacg gattgcggac aaaatctccg
    11341 ggtcggtaat gccccactgg gtctgggcca ggatggcggc ggctatgccg tgccacaccg
    11401 gcgaagggcg tgcaggggca ttttctgcta ttatagcatt atcggcgaaa atctgcaaca
    11461 attcttgttt aggcagttcc ttggcggaat catgcaaaat tgccgccata gcggcctttt
    11521 ccgggtcagc accccagcgt ttggcaagtt ttactgccat tttttttaca ttcttcgtgt
    11581 gggtccagcg tttttcgccg agtctggact tcacaagctt cttggcttcg tcgtaatcca
    11641 tgctatatat tcccctctcc ttggcggtag gggccgggca tgcccggccc tcggccttgc
    11701 ctctattgcc cttgaacggg ttatgatttt tttgctgcgg gccgggcatg cccggcccct
    11761 acagtgctac aagctgccgt aaagccggtt ttggcggata accgccaaca cttccggcgg
    11821 caaaacctcc ggccactttt ccggcggtat cttcccgctg cggatatcgc tggacgcaca
    11881 ggggtagctt tcggcctggg caaacaacac cgtgccgccc gcctgttcca gcttggcagc
    11941 agcggcgtgc agggcggcat cgtccgtgcc ggtacggctt tccaccacca gcgcggccat
    12001 ttttaacata tcctgccagc ggtaccactc gccgaacgtc agcagcatat cgctgccgac
    12061 gcaaagatac agttccgcgc cggggttggc gccccgcagc atttccagcg tgtggacggt
    12121 gtagctgcgg ccgccctggc gtatctccca atcgctgact tccactgccg gggacagcgc
    12181 cctaaagcac tggcacatct gcaaacgcag ttccgccggg gtggcgctgg cgtgtttgtg
    12241 gggcggcgtg cctgccggca tgacgatggc ccggtcgggc cgcacccgtt ccagcgcagc
    12301 ccgcaggttg ttcttatggc cgttgtgggg cgggtcgaag gtgccgccgt agagcagtat
    12361 cttcattttg ccagcacagc ggcgtaggcg ctttcctttt tcttttgctt gaacaggacg
    12421 aacttgcggc caatgacctg cacgcactcg cagccgacgg cctcacagag gatatcggcg
    12481 gcctggcggg ggtcatattc gctgttttcc aagactttga ccttgataag ctcgcgtgcg
    12541 gcgatgcagt cggcaatccc ttggatcagg gtctcgtcga tctcgccctt gccgatctgg
    12601 tagacagggt ccatggtgtt ggctgcgccg cgcagtgcag cgcgctgttt gctggttaaa
    12661 gacatagggt tgctccttta taattatcgt atatatcgta tcgcggtagg ggcagggcac
    12721 gcccggccct cggccttgcc ttgcctgccc ttgaacgggt agtatttttc atgttgaggg
    12781 tcgggcatgc ccgaccccta cggactgcta ttacttcaaa aacgcgggca gcttttcttc
    12841 cagcttctcc atcgcccggc ctctatggct gatggcgtcc ttctcgtctg gggtaagctg
    12901 ggcatagctg cgggcctcgg cgttggggtg cttgccatcg ggtgcgccat actcgtcagg
    12961 gatgaacagc gggtcgtagc caaagccgta atcgccgttc tgcaggctga atgcaatgcg
    13021 gcccgggcac tcgccctcga cttccagtgc gcggccatcg ggcagcataa agcagaccgc
    13081 cgagacaaac tttgcgccgc gctgttcggc gggcacatcg gccatttctt gcagcagctt
    13141 tgcgttgttg gcgtcgtcat cgccgtggtg cccggcatag cgggcgctgt acacgccggg
    13201 cgcaccgtcc aatgcatcgg tgcaaaggcc cgagtcatcg gccacggtgg gcaggccgct
    13261 ggccttgcag aaggcctcag ccttgatgcg ggcgttttcg gcaaacgtcg tgccggtctc
    13321 ctcggggtcc agggcgatgc caagctcccg cagactcttg acctcatgcc ccatgcgttc
    13381 caaaatgcgg cgcagctctt tcagcttgcc cgcattgttg ctggcggcgc agacgatcat
    13441 gcttcgccct ccccttcttc tttctgttcc tctttctgca gggccgattc cggcagcagg
    13501 ctttccacaa aggtctgggc gtcaaactcc atcaggtcgt cgattttctc gccgacaccg
    13561 atgaaccgga tgggcaggcc cagattctcc cacaccgaaa ccgcgcagcc gccgcggggc
    13621 gtgccgtcca gcttggtcag caccacgccg gtggcgctgg cagccttgca gaactcctcg
    13681 gcctggctga tggcgttctg gccggtgatg gcgtccagca ccagcagcac ctccaggctg
    13741 gcctcggggc tggccttttt gacgctgcgg gagatcttgg acagctcgtc catcaggccc
    13801 tttttgttgt gcaggcggcc tgcggtatcg gcaatgacca tgtcatagcc gcgggcgttc
    13861 gcactcttga ccgcatcaaa gatgacggcg gcgggatccg cgcccatgcc tgcctgcacc
    13921 agcggcacac cggcgcggtc agcccagagt tccagctgct cgctggcagc ggcgcggaag
    13981 gtatcgcccg cagccagcat cacgcggcgg ccctgctttt tgtacagcgc ggccagcttg
    14041 gcgatggtcg tggtcttgcc cacaccgttg acgccgatga cgagaatgac ggcgggcttg
    14101 ccgtccagct ccatcgggta gcggggggtc agctcctggc agatgatgcc gcgcagggtg
    14161 tccagcgcct cctggccggt tttcaggtgc ttttcctcga cctcgtcgtg cagctcatcc
    14221 accaggcgca cagcgcaggc ggggccgacg tcggccagaa tcaactgctc ttccagctgg
    14281 tcgtacaggt cgtcgttgat ctgcgcgtgg gtcagcgtgt tgacgatgtt ctggaaaaag
    14341 ccggtgcggg tcttttccag cccgcgcttg accttttgct gttcctgaaa ttcttcttct
    14401 gaaatatcac gtttgaaaaa gttgaaaaag cccatggcgg cctccttttt ttagggcaat
    14461 accgcacaat tctaagtgcc gatacggcga gcgaggtgcg gcagctgcta agccaaaagc
    14521 gcagataata ctttgtgtat tatcgagcat tttggcaacg cagatgcc
//
LOCUS       DBMDSQ010000022        14453 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_1314_length_14453_cov_2.171962, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000022 DBMDSQ010000000
VERSION     DBMDSQ010000022.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 14453)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 14453)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14453
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1314_length_14453_cov_2.171962"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..612)
                     /locus_tag="ACZPAX_01640"
     CDS             complement(<1..612)
                     /locus_tag="ACZPAX_01640"
                     /inference="COORDINATES: protein motif:HMM:NF013429.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HIR1112633.1"
                     /translation="MANYQLAISNIAWLKDDDEAVYTAMQQAGFTGLELAPTRIFSEA
                     PYENLTSALLFGGYLKNRWGFSVPSLQSIWYGQKGNIFNPADTEPLLDYTAQAFQFAH
                     SLNCPSLVFGCPKNRMRPLGGNDAAAEAFFMQAGNLAARYGVHLALEANPPMYTNYLN
                     DTADAFALVKRLDNPGLSVNFDLSTVLAQGEKLQTFIDDMQYVS"
     gene            complement(616..1542)
                     /locus_tag="ACZPAX_01645"
     CDS             complement(616..1542)
                     /locus_tag="ACZPAX_01645"
                     /inference="COORDINATES: protein motif:HMM:NF016228.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar nucleotide-binding protein"
                     /protein_id="HIR1112634.1"
                     /translation="MSCDLLVGSTGFVGGNLAAKHTFTAACHSSDIAAQYGTRPDLCV
                     YAGVPAAMFLANADPEADLAVMRAARENIRRIAPQQLVLISSIAVLADSRGVYEDSPA
                     QPAENLPAYGKNRLQLEQWVREDFPDALIVRLPALYGIGIKKNFLFDLHTITPAMLKP
                     EKYDELAAKSPLVQSAYTLADNGFYKLNGTVDAAALKAFFAGNDFNALAFTDSRSRYQ
                     FYNLRRLWPDLEKARAADLKLLHLCTPPITAGEVYTAVTGRNWHNELAKPPFDYDLRS
                     RHADILGGSGDYLCTKQQELDDITRFLRDWRD"
     gene            complement(1729..2376)
                     /gene="hisH"
                     /locus_tag="ACZPAX_01650"
     CDS             complement(1729..2376)
                     /gene="hisH"
                     /locus_tag="ACZPAX_01650"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HIR1112635.1"
                     /translation="MKVTIIDYGLSNLLSVQHAFAHFGAETLLTSKPEDVLAADALVL
                     PGVGAFQDGMAGLEKLGLVEPIRQKAAAGTPLLGICLGMQMLFDESEEFGLHKGLGLI
                     PGRVVKIPDTDADGCPQRVPHISWNPLYPGGSRADFGGTVLAAVQPGEECYFIHSYEA
                     KPTDETDCLAFTVYGGRRICAAATHGSVLGCQFHPEKSGEVGLKIIEEFMKSADR"
     gene            complement(2373..3140)
                     /gene="hisF"
                     /locus_tag="ACZPAX_01655"
     CDS             complement(2373..3140)
                     /gene="hisF"
                     /locus_tag="ACZPAX_01655"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HIR1112636.1"
                     /translation="MLKKIRLIARLDVKDTNLVKGIQLEGLRKLGDPNAFAKEYYEQG
                     IDELLYIDIVASLYNRNNLSDIVRKTVDDVYIPVCVGGGLRSVEDVRHILAMGADKVA
                     INTAAIKRPELITEVAEAFGSQCMVLSIQAKRSRTWPGKWEAYYDNGRAHSGYDVVEW
                     AKRGVALGAGEILLTSVDNEGLQQGMDLELIEAVTKAVNVPVICGGGVGCGDDIVDAA
                     NAGADAVACAAVLHYKKETVQELKDDIRAGGVEVRRV"
     gene            complement(3185..4558)
                     /locus_tag="ACZPAX_01660"
     CDS             complement(3185..4558)
                     /locus_tag="ACZPAX_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl sugar amidotransferase"
                     /protein_id="HIR1112637.1"
                     /translation="MEKIPRHVDIDYSKYAPDIPEEQLEAYYGLPKHVQFCNECVMSN
                     QKPNSCYEFEHTIKSIKKTMVIQDDGVCDACHACHNKANGHVDWAEREKELRELCDQY
                     RKNDGSYDCLVPGSGGKDSFYAAHLLKYKYGMHPLTVTWAPHIYTPWGWENMQAWIHA
                     GFDNYLCTPNGMTHRLLTRLATENLFHPFQPFILGQKQLAPKMAAKFGIPLVFYGENE
                     AEFGNPIADNNSALRDEHFFAVNDYDHIYLGGVSLRQLEEDYKVDKADLAIYLPSETS
                     NLEKNHIQVRYLGYYEKWHPQGAYYYSVEHGGFRPAPERTQGTYSKYNSIDDKIDDFF
                     YYTTYIKYGIGRTTYDAAQEIRNEEITLDEGKALCKKFDGEYPDRFEKEIFKYLSLDR
                     QHFPWASQLFEQPKMDRDYFMDLADRFRSPHIWKWEDGMWKLRHTPYEGDSEVLWGDP
                     RGTHHEI"
     gene            complement(4650..5507)
                     /locus_tag="ACZPAX_01665"
     CDS             complement(4650..5507)
                     /locus_tag="ACZPAX_01665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1112638.1"
                     /translation="MTRPAEHPGAARLSVMVQLVGSVCAVLLALSLALAATLLREGHF
                     AACIENSGYLDTLHTAVLDTCGGYAAAAGTTADPLADIITPEAVRAGVLHRADGLWHG
                     STVDETDPFNDLIVTYQDNVAPEPAVYDGYRILQYNCQMEWKSAMATPFSAAISMVLQ
                     YRTVVAVTMYLSALLLVGCIALQYRLAARWRVFCRGLLHMADACLLAGAVLAGWLGFG
                     SGYRSWTAVGDIGYTLFCRWFGALAPAVLGCAALVAAIPTGLAFWVCVQYHRAKAQRP
                     SCKTEKPVL"
     gene            complement(5504..6208)
                     /locus_tag="ACZPAX_01670"
     CDS             complement(5504..6208)
                     /locus_tag="ACZPAX_01670"
                     /inference="COORDINATES: protein motif:HMM:TIGR01076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class D sortase"
                     /protein_id="HIR1112639.1"
                     /translation="MKETHSGKAVSSHSKRKNVVLSLVFCVAAVCVALLVLVGPGHSL
                     AGAAQRSGVDAALPASIDTTTSDNTAQPTEAPTAPPQPAAVNINDFGGYPSAGDALGE
                     IKISGTSVDCTLYYGDEDAQLDAGAGIYPGSKIPGEGGTILVAGHTATFFRDFEHAQN
                     GAQITVTTAYGTYVYEITDMQVATDTDTSAYDLDAAEENIILYTCYPLGQTALTDLRY
                     FIYGRFVSGPALEGLS"
     gene            complement(6226..7176)
                     /locus_tag="ACZPAX_01675"
     CDS             complement(6226..7176)
                     /locus_tag="ACZPAX_01675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1112640.1"
                     /translation="MKKFLAMLCAVALAALCAVSAFAEATPGQAANELATNILNTANK
                     GIMVPEAVEMTEHIAANPTIPVTKEQANAAMGVMNAVGNTVVKKVATGSKVSNAISFE
                     SYKENGVVVNSLTRKDDKTLVLDASWSGNEAQPEMGVMVAVYGLESRAGYQLVYVADD
                     GTRTTYDTQLSSANNAVVFWIPHFSTYEIVRVRDYVEPSDDSQTDADRAAQAAAAAAE
                     AARVDAQFYTCPACGYHNWTGTVGGYKCDHCGHIEAKDLSSYPNVKGTATLAAASSTK
                     AVGTNPIKATGADMNLMVLAVLALAAAMVGGFACVARKHK"
     gene            complement(7385..8947)
                     /locus_tag="ACZPAX_01680"
     CDS             complement(7385..8947)
                     /locus_tag="ACZPAX_01680"
                     /inference="COORDINATES: protein motif:HMM:NF016800.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HIR1112641.1"
                     /translation="MHLPERPLRCAPAAIAGNVLTLAALLLLPLYMPSGYVGLVDAKF
                     HLLLWLAGAGAALLVWRLWLGLRGRAPAVRLDLATALPLPVLCLSYTVAWFFAEDRAL
                     ALWGLAGRYNGLLMLLACTVLYFVVRLVGGGVPSVWFGRLLAGAGCAVTLLSWLNFFM
                     ADPLDAYYSFLPGRGGLFLGTVGNINFYGALLDICLPIAVWELLTAPDRDTARRWGAV
                     SVCLGTGLIAAGSDAAWLGAVCAVWALCMARRVTAGRLARLLFAGGAWALCAAAAGLL
                     ARWLPIRTEWRTLSGLVTQLFPALAVAGVLFALGALLRRWPTARSWRAARVLGVLTVL
                     LAAELFVAANFTSILPESLAVSLRFDDQWGSNRGFAWKRLWTVYKDDLTPLQMVFGLG
                     GDAANARLNIDDYSVKYMTLLNGDVFDSAHNEYLQHLLCGGAVGLAAWLAFLAGHIRR
                     GLRTAPGLALAVFGYAVQAFFSISMPGVLPLVFVLAALCVKSQPLAARGEYRCLLGLA
                     LAAPPVMLLAAL"
     gene            complement(8972..10129)
                     /locus_tag="ACZPAX_01685"
     CDS             complement(8972..10129)
                     /locus_tag="ACZPAX_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014183171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HIR1112642.1"
                     /translation="MTTLIRGGLIHDAVHRGAYIADILLAGGKIAGIGTDLTAPADAE
                     IFDADGLDVYPGFVDAHTHIGLDGYGIGYEGCDYNEMNDIWTPQLRAIDGINPRDPSL
                     AIARKAGVTCVCTGPGSANVLGGTFLAMKTVGDRIDNMIVKDPVAMKCAFGENPKRCY
                     RDKCDSTRMSTAAFLRGALMQAKDYGARKQAANGDVTKMPAYNQKLEALLPVLAGEIP
                     LKAHAHQANDIFTALRIAHEFGLRITLEHVTEGHLIVDELAKEKDVPMAVGPSLTHAS
                     KFELQNKSWATPGVLAKAGCHVSIITDNSVIPQQYLPLCAGMAVKAGMDEFDALKAIT
                     INPAEHIGVADRVGTLEVGKDADVVITNGCPFEVMTETRAVFIDGNRVDTL"
     gene            complement(10247..10549)
                     /locus_tag="ACZPAX_01690"
     CDS             complement(10247..10549)
                     /locus_tag="ACZPAX_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005354918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HIR1112643.1"
                     /translation="MVRHVILWKLKDMPDAEKAAVKADIKAGLEGLVGKIPGLLEVHV
                     YTDALPSSANADLMLDTTFTDAAALKGYSTHPAHVAVADGKVRPYTAVRTCLDFNV"
     gene            complement(10549..11670)
                     /locus_tag="ACZPAX_01695"
     CDS             complement(10549..11670)
                     /locus_tag="ACZPAX_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl sugar amidotransferase"
                     /protein_id="HIR1112644.1"
                     /translation="MRYCKKCTMPDTRPGITFDAEGVCSACRHYENRKNVDWDARFKE
                     FEAYCDKYRGMNGPGGYDCAVAVSGGKDSHFQVWMLKEVMHMNPILFSVEDNFPMTQA
                     GIHNLKNISEEFGCTIISCKPNIKVQKVLMRKFFEKYGKPTWYVDRLIYTFPLHMAAK
                     FNTPMLCYGENVSFEYGGNADKETYSAMDQIENGVAVGMPMDELLGDGVTEKDLSLTL
                     APSAEERAKLDPFYMSYFVPWNSYKNYQIAKAHGFHDLTHEWDRTHHAENFDQIDSRA
                     YLVHSWLKYPKFGHAAATDYTARYIRYGMMTREEAIPIIKERDGKLDPLCVRDFCDFC
                     GYTETEFWAIMDKFYNRDLFEKNEYDEWQLKHPIWEENK"
     gene            complement(11784..13412)
                     /locus_tag="ACZPAX_01700"
     CDS             complement(11784..13412)
                     /locus_tag="ACZPAX_01700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1112645.1"
                     /translation="MKQQTKGGALHKVWQALPWLWMAAAYLFDLWYQLVPGKWIVDSD
                     LASEMILSDLLNKEGTIISHNWFYSTELKVVNLQWFYRLGLLIFPNDWHLARTFGMAI
                     TLALFAAAMLFFVKCAGLGRAGLWMVGTLLWPFGQHYLVYAIYGGYYLVYTFFYMLVL
                     ALVLRSLNADKKHCALQWVLACVITAVAGMNGVKQLMVFHAPLCLAAAILLVLALHSC
                     GKTDWKAALDVCRKEVRLLAASLVTAVAAAAGYFVSNAVLSRMYDFKSYNFIVWNRDE
                     DWFTLDRILMDFFHEFGYENGSGVFHFGGIAAAVGLLLGCWMFFCIVRLLLRLDKLER
                     NDKLLVLLLVAMLAVCGVAYTYFHEYYLYFWLMNMPVAIAVMAVEIKTEDFHILGARQ
                     LLGVGLAACFTLCAVSTVRQEQEHPYLAHKGLNTAAEWLVDNGYTQGYSTFWNGNAMT
                     ELTSGKLEVWTLQSLDRDDVPNWLQPKSHLTTDPEHPFLLIDTETDGPAENAKLIQYG
                     DCTEVYNDGRYVIYDFADADALHAAAQAAADAKQ"
     gene            complement(13634..>14453)
                     /gene="gpmI"
                     /locus_tag="ACZPAX_01705"
     CDS             complement(13634..>14453)
                     /gene="gpmI"
                     /locus_tag="ACZPAX_01705"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005922312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HIR1112646.1"
                     /translation="VVTCEGGRVEDGDTVIFMNFRPDRARQMTRIFCDDAFTGFERRG
                     GRKQVNYVCMAEYDATMPNCEVAYPPVELKNTLGEYLAANGKTQLRIAETEKYAHVTF
                     FFNGGVEQPCEGEDRKVIPSPKVATYDLKPEMSAYEVADECKARIESGKYDVIILNFA
                     NCDMVGHTGVFDAAVKAVEAVDKCVGEVTDAVLNAGGVVFLTADHGNAEKMKNPDGSP
                     FTAHTTNVVPFAVIGAGDVKLREGGCLADIAPTMLPYIGLPVPAEMTGKSIIVE"
BASE COUNT         2757 a         4359 c         4490 g         2847 t
ORIGIN      
        1 gctgacatac tgcatatcat cgatgaaagt ttgcagtttc tcgccctggg ccagcacagt
       61 ggacaaatca aagttgaccg acaggccggg gttatccagc cgcttgacca acgcgaaagc
      121 gtccgccgtg tcgttcagat agttcgtgta catcggcggg ttggcctcca gtgccagatg
      181 cacaccgtac cgcgccgcca ggttgcccgc ctgcatgaag aacgcctcgg cggcggcatc
      241 gttgccgccc agcggacgca tacggttttt ggggcagccg aacaccagcg aggggcagtt
      301 caggctgtgg gcaaactgga atgcctgggc cgtgtaatcc aacagcggct cggtgtcggc
      361 gggattgaag atattgccct tctggccgta ccagatgctt tgcaggctgg gcaccgagaa
      421 gccccagcgg tttttgagat agccgccgaa cagcagcgca ctggtcagat tttcatacgg
      481 tgcttcggag aaaatgcggg tgggggccag ctccagcccg gtgaagcccg cctgctgcat
      541 ggcggtgtag acggcctcgt cgtcgtcctt gagccacgca atgtttgaga tggcaagctg
      601 atagttcgcc atgggtcaat ccctccaatc gcgcagaaag cgcgttatat cgtcaagttc
      661 ctgttgtttg gtacacaggt agtccccgct gccgcccaaa atgtccgcat ggcggctgcg
      721 cagatcgtag tcaaacggcg gcttggcaag ctcgttgtgc cagttcctgc cggtgacggc
      781 ggtgtagacc tcccccgccg tgatgggcgg cgtacagagg tgcagcagct tcaagtcggc
      841 ggcgcgggct ttctcaagat ccggccagag ccgcctcagg ttgtaaaact ggtagcggct
      901 gcgggaatcg gtgaaggcca gtgcgttaaa atcgttgccc gcaaaaaacg ctttgagcgc
      961 tgcggcatcc actgtgccgt tcagcttgta aaagccgttg tccgccagcg tgtaggcgga
     1021 ttgcaccaac ggcgattttg ccgccagttc gtcgtatttt tccggtttca acatagccgg
     1081 ggtgatggtg tgcaggtcaa agaggaagtt ctttttgatg ccgatgccgt agagcgcggg
     1141 cagccgcacg atgagtgcat cggggaaatc ctcccgcacc cactgctcta attgcaggcg
     1201 gtttttgccg taggcaggca ggttttcagc aggctgggcg gggctgtcct catagacgcc
     1261 gcggctgtct gccagcacgg caatactgga aatcaggacg agctgctgcg gcgcgattct
     1321 tcggatattt tcccgcgcgg cacgcatgac agccaagtct gcctccgggt ccgcgttggc
     1381 caaaaacata gcggccggca cgcccgcata gacgcagaga tccgggcggg tgccgtactg
     1441 cgcggcgata tcgctgctgt ggcaggccgc ggtgaaggta tgcttcgccg ccaggttgcc
     1501 ccccacaaag ccggtggagc cgacaagcag atcacaagac atggctgatt cccctttgca
     1561 catttataca agataattat accgtattgc gggggaaaaa gcaacgccct gtgcaggatt
     1621 gcacagggcg ttgcagatga ttgctctgta ggggccggac atgtccggcc ctccacggta
     1681 cggcaggggg ctcttacggt aaagccgcgg gccgggcata cccggcccct acctatctgc
     1741 cgacttcata aactcctcta taatcttcaa ccctacttcc cccgattttt ccgggtggaa
     1801 ctggcagccc aggacgctgc cgtgggtggc ggcggcacag attcggcggc cgccgtagac
     1861 cgtgaaggcc aggcagtccg tttcgtcggt gggcttggcc tcgtagctgt ggataaagta
     1921 gcactcctcg ccgggctgca ccgccgccaa caccgtgccg ccaaaatcgg cacggctgcc
     1981 gccagggtac agcgggttcc agctgatgtg cggcacccgc tgggggcagc cgtcggcgtc
     2041 ggtgtcgggg attttcacca cgcggcccgg gatcagcccc agacctttgt gcagaccgaa
     2101 ctcctccgac tcgtcaaaca gcatctgcat tcccaggcag atgcccagca gcggcgtacc
     2161 ggcagcggct ttttggcgga tcggctccac caggcccagt ttttccagcc cggccatgcc
     2221 atcctggaat gcgcccacgc cgggcagcac cagcgcgtcg gcggccagca catcctccgg
     2281 cttgctcgtc agcagtgtct ccgcgccaaa atgcgcgaag gcgtgctgca cgctgagcag
     2341 gttggacagg ccgtagtcaa taatcgtcac tttcataccc ggcgcacctc caccccgccc
     2401 gcgcggatgt catccttcaa ctcctgcacg gtttcttttt tataatgcag caccgcagcg
     2461 caggccacgg cgtccgcgcc cgcgttggcg gcatcgacga tatcatcgcc gcagcccaca
     2521 ccgccgccgc agatgaccgg cacgttcaca gccttggtca cggcctcgat gagttccagg
     2581 tccataccct gctgcaagcc ctcgttgtcg acgctggtca gcaggatctc gcccgcgccc
     2641 agcgccacgc cgcgcttggc ccactcgacg acgtcatacc cggagtgggc gcggccattg
     2701 tcgtagtagg cctcccactt gccgggccag gtgcggctgc gcttggcctg gatggacagc
     2761 accatgcact gactgccgaa agcctcagcc acctccgtga tgagttcggg ccgcttgatg
     2821 gccgccgtgt tgatggcgac cttgtccgca cccatcgcca ggatgtggcg cacatcctcg
     2881 acgctgcgca agccgccgcc cacgcagacc gggatataca catcgtcgac ggttttgcgc
     2941 acgatatcac tcagattgtt gcggttgtac aggcttgcca cgatatctat atagagcagt
     3001 tcgtcgatgc cctgctcata gtactctttc gcaaaggcgt tggggtcgcc cagcttgcgc
     3061 agcccttcca actggatgcc tttgacaagg ttggtgtctt tgacgtcaag acgggcgatc
     3121 agtcgtattt ttttcagcat aagtgaacct cgttaaagcc ttctcctgag ggagaagccc
     3181 tttgtcaaat ctcgtgatgc gtgcctctcg ggtcgcccca cagaacctcg ctgtcgccct
     3241 catagggggt gtggcgcagc ttccacatac cgtcctccca cttccagatg tggggagagc
     3301 ggaagcggtc ggccaggtcc atgaagtaat cccggtccat cttgggctgc tcaaacagct
     3361 ggctggccca ggggaagtgc tggcgatcca gcgacagata cttgaaaatt tccttttcaa
     3421 agcggtcggg gtactcgccg tcgaacttct tgcagagcgc cttgccttcg tccagcgtga
     3481 tttcctcgtt gcggatctcc tgcgctgcat cgtaggtcgt gcggccgatg ccgtacttga
     3541 tgtaggtggt gtagtagaag aaatcgtcga ttttgtcgtc gatggagttg tacttggagt
     3601 aggttccctg ggtgcgctcc ggcgcggggc ggaagccgcc atgctcgaca ctgtaatagt
     3661 acgcgccctg cgggtgccac ttttcgtagt agcccagata ccgcacctgg atgtggtttt
     3721 tctcgaggtt ggacgtctca ctcggcagat aaatcgccag gtcggcctta tcgaccttat
     3781 agtcctcctc cagctggcgc aggctgacgc cgcccaggta gatatggtcg taatcgttga
     3841 cggcgaagaa gtgctcatcg cgcagggcgg agttgttgtc ggcaatgggg ttgccgaact
     3901 cggcctcgtt ctcgccgtag aacaccagcg ggatgccgaa cttcgcggcc atcttggggg
     3961 caagctgctt ctggcccagg atgaacggct ggaatgggtg gaacaggttc tcggtggcaa
     4021 ggcgggtcag caggcggtgg gtcataccgt tgggggtgca caggtagttg tcaaagcccg
     4081 catggatcca ggcctgcata ttctcccagc cccagggggt gtagatatgc ggggcccagg
     4141 tcacagtcag cgggtgcatg ccgtacttgt atttcagcag atgggcggcg tagaagctgt
     4201 ccttgccgcc ggagccgggc accaggcagt cgtagctgcc gtcgttcttg cggtactggt
     4261 cgcagagttc gcgcagttcc ttctcgcgct cggcccagtc gacgtgacca ttggccttgt
     4321 tatggcaggc atggcaggca tcgcagacgc cgtcgtcctg aatgaccatc gtctttttga
     4381 tggatttgat ggtgtgctca aactcatagc aggagttggg cttttggttg ctcatgacgc
     4441 actcgttgca gaactgcacg tgcttgggca gaccgtagta ggcttcgagc tgctcctccg
     4501 ggatatcggg ggcgtacttg ctgtaatcaa tgtccacatg gcggggaatc ttttccatat
     4561 tctttacact ccaatcggct ctgtatcaaa atgaaaaggc gccgtccgat agcaatgcta
     4621 tcagacggcg cctttgccgt atggatgtat tataacacag gtttttcggt tttgcaagag
     4681 gggcgctgcg ctttcgcgcg gtgatattgc acgcagaccc agaaagccag cccggtcggg
     4741 atggccgcca ccagcgcggc gcagcccagc acagccgggg ccagcgcacc gaaccagcgg
     4801 cagaacagcg tatagccgat atcccccact gctgtccagc tgcgatagcc gctgccgaag
     4861 cccagccacc cggccagcac tgcgcccgcc agcaggcagg catcggccat atgcagcagg
     4921 ccgcggcaga atacccgcca tctcgccgcc aggcggtatt gcagcgcaat acaccccacc
     4981 agcagcagcg ccgagaggta catcgtcacg gccacgacgg tgcggtattg cagcaccata
     5041 ctaatagccg ccgaaaacgg cgttgccatg gcgcttttcc attccatctg gcagttgtac
     5101 tgcaagatgc ggtagccatc gtagacggcg ggttccgggg cgacgttgtc ctggtaggtc
     5161 acgatgaggt cattgaaggg gtccgtctca tcgacggtgc tgccgtgcca caggccgtcg
     5221 gcccggtgca acaccccggc gcggacagcc tccggcgtaa tgatgtccgc cagcgggtca
     5281 gcggtcgtac ccgctgccgc agcgtaccca ccgcaggtgt ccagcacggc ggtgtgaagg
     5341 gtgtccaggt agccactgtt ttcgatgcag gcggcaaagt gcccctcccg cagcagcgtg
     5401 gcggccagag ccaggctcag ggccagcagc accgcgcaga cgctgcccac cagctgcacc
     5461 atgaccgaga gccgcgccgc gcccgggtgt tctgcggggc gggtcaagac agaccctcca
     5521 gcgcagggcc gctgacgaag cggccgtaga tgaagtagcg cagatcggtc agcgcggtct
     5581 ggcccagcgg gtagcaggtg tagaggatga tgttttcctc cgccgcgtcg aggtcgtagg
     5641 ccgaagtgtc ggtgtcggtc gcaacctgca tatcggtgat ttcgtagacg taggtaccgt
     5701 aggcggtggt gacggtgatc tgcgcgccgt tctgggcatg ctcaaaatcg cggaagaagg
     5761 tcgccgtgtg tccggccacc agaattgtac cgccctcgcc ggggattttg ctgcccgggt
     5821 agatgcccgc gcccgcgtcc agctgggcat cctcgtcgcc atagtacagc gtgcagtcca
     5881 ccgacgtgcc ggagattttg atttcgccca aagcgtcccc cgcactgggg tagccgccga
     5941 aatcattgat gttcaccgcc gcaggctgcg gcggtgccgt gggggcctcg gtgggctggg
     6001 cggtgttgtc cgaagttgtc gtatcgatgg aggcgggcag cgcagcgtcc acgccggagc
     6061 gctgggccgc gcccgccagg ctgtgcccgg ggccaaccaa aaccagcagt gccacgcaaa
     6121 ctgcggccac acaaaaaaca agagaaagaa caacgttctt cctcttgctg tgtgatgaca
     6181 ctgctttgcc ggaatgtgtt tccttcataa gcagtttatc cttttttact tgtgcttgcg
     6241 ggcaacgcag gcgaagccgc cgaccatagc cgcagccaga gccagaacgg ccaggaccat
     6301 caggttcatg tcagcgccgg tagccttgat ggggttggtg cccacagcct tggtagagga
     6361 agcggcggcc agggtggcag tgcccttgac gttggggtag ctgctcaggt ccttggcctc
     6421 gatgtggccg cagtggtcgc acttgtagcc gcccacagtg ccggtccagt tgtggtagcc
     6481 gcaggccggg caggtgtaga actgggcgtc aacgcgggca gcctcggcgg cagcagcggc
     6541 tgcctgggca gcgcggtcag cgtcggtctg gctgtcgtcg ctgggctcaa catagtcgcg
     6601 gacgcggacg atctcatagg tggagaagtg ggggatccag aatacgaccg cattgttggc
     6661 ggaagacagc tgggtgtcgt aggtggtgcg ggtgccgtcg tcagcgacgt aaaccagctg
     6721 gtagcccgcg cgggactcca ggccgtacac ggcgaccata acgcccatct cgggctgggc
     6781 ctcattgccg gaccaggaag catccagcac gagggtcttg tcgtccttgc gggtcaggct
     6841 attgacgaca acgccgttct ccttgtagga ctcaaagctg atggcattgg aaactttgct
     6901 gccggtggca accttcttaa cgacggtatt gccgacagcg ttcataacgc ccatagccgc
     6961 gttggcctgc tctttggtga cagggatggt gggattagca gcgatgtgct cggtcatctc
     7021 aacagcctcg ggaaccatga tgcccttgtt ggcggtgttc aggatgttgg tggccagctc
     7081 gttggcggcc tggccggggg tagcctctgc aaaagcggag acggcgcaca gagccgccag
     7141 cgccacagcg cagagcatgg caaggaattt cttcatttgg gtaggactcc tctcaatagt
     7201 gtttgtttac tttgcagtaa atttcaagac tattgaaagt ataccccccc ccattagctt
     7261 ttgtcaattt acattttcac caataatcca cattttcttt cacacttttg tacactattt
     7321 agggggtcac agcgttttca acgcagagcc ccgcccctac cgcacatttt tcgatacgat
     7381 ttttttatag cgccgccagc agcattaccg gcggggcggc cagggccagg cccagcaggc
     7441 atcggtactc tccccgggca gccaggggct gcgactttac acagagggcc gccaacacaa
     7501 acaccagcgg cagcacaccg ggcatactga tactgaaaaa cgcctgcacc gcatacccga
     7561 aaactgccag tgccagcccg ggcgcagtcc gcagcccacg gcggatatgc ccggccagaa
     7621 atgccagcca ggcagccagg cccaccgcac cgccgcaaag caggtgctgc aaatactcgt
     7681 tgtgggccga gtcaaaaacg tcgccgttga gcagcgtcat atatttgaca gaataatcat
     7741 caatgttcag ccgcgcgttg gctgcgtcgc cgcccaggcc gaacaccatc tgcagcggcg
     7801 tcaaatcgtc cttgtagacg gtccaaaggc gcttccacgc aaagccgcgg ttggaacccc
     7861 actggtcgtc aaaccgcagc gagacagcaa gggattcggg cagaatgctc gtgaaatttg
     7921 ccgctacaaa cagctcagcc gccagtaaaa cggtcaggac acccagcacc cgcgcggctc
     7981 gccagctgcg ggccgtgggc cagcggcgca gcagtgcgcc cagcgcaaaa agcacccctg
     8041 ccaccgccag cgcggggaac agctgtgtga caagcccgga cagggtgcgc cattcggtgc
     8101 ggatgggcag ccagcgggcc agcagacccg ccgcagcggc gcagagcgcc caggcccctc
     8161 ccgcgaagag cagccgggcc agccgccccg ccgtgacacg ccgtgccatg cagagcgccc
     8221 acaccgcgca aacggccccg agccaggcgg cgtcgctgcc cgccgcaatc agcccggtgc
     8281 ccaggcaaac ggacaccgcg ccccaccgcc gggcggtgtc ccggtcgggg gcagtcagca
     8341 gctcccacac agcgatgggc aggcagatat ccaacagtgc accgtaaaaa ttgatgttgc
     8401 ccactgtgcc cagaaatagc ccgcccctac cgggcagaaa gctgtaatag gcgtccagcg
     8461 gatctgccat gaaaaagttc agccagctca acagcgtgac ggcgcagccc gcgcccgcca
     8521 gcaaccgccc gaaccacacc gagggcacac cgccacccac cagccgcacc acaaagtaaa
     8581 gcaccgtgca ggcaagcagc atcagcaggc cgttgtaccg tcccgccagc ccccacaggg
     8641 ccagggcgcg atcctcggca aagaaccagg ccaccgtgta gctcaaacag agcaccggca
     8701 acggcaatgc ggttgccaaa tccagccgaa cggcaggggc acggccgcgc aggcccagcc
     8761 acagccgcca gaccagcagc gcagcgcccg cccccgccag ccagagcagc aggtgaaatt
     8821 ttgcatccac aaggcccaca tagccgcttg gcatatacag cggaagcagc aaaagcgccg
     8881 ccaacgtcag cacattcccg gcaatggctg cgggggcgca gcgcagcggg cgttcaggca
     8941 ggtgcataca aaagttcctt tcagcgttat tttacagggt atcgacgcga ttgccgtcga
     9001 tgaaaacagc ccgcgtctcg gtcatgacct cgaaggggca gccgttcgtg atgacgacgt
     9061 ccgcatcctt tccgacttcc agcgtgccga cgcggtccgc cacgccgata tgctccgccg
     9121 gattgatggt gatggctttc agcgcatcaa actcatccat gcccgccttg acggccatac
     9181 cggcgcagag cggcagatat tgctgcggga tgaccgagtt atcggtgatg atggagacat
     9241 ggcagcccgc tttcgccagc acaccgggcg tggcccaaga tttgttctgc aattcaaact
     9301 tgctggcgtg ggtcaggctg ggaccgacgg ccatgggcac gtctttttct tttgccagct
     9361 cgtccacgat gaggtggccc tcggtgacat gttccagcgt gatgcgcagg ccgaactcat
     9421 gggcaatgcg cagcgcggta aagatatcgt ttgcctggtg ggcgtgggct ttcaggggga
     9481 tctcccctgc cagcaccggc agcagggcct cgagcttctg gttgtaggcg ggcatcttgg
     9541 tcacatcgcc gtttgcggcc tgcttgcgtg cgccgtagtc cttggcctgc atcagcgcac
     9601 cgcgcaggaa cgccgccgtg gacatacggg tcgagtcgca cttgtcgcgg tagcagcgct
     9661 tggggttctc accgaaggcg catttcatgg caacagggtc tttgacgatc atgttatcga
     9721 tgcggtcgcc gacggttttc atggccaaaa acgtgccgcc cagcacattg gcgctgcccg
     9781 ggccggtgca gacgcaggtg acgcccgcct tgcgggcaat ggccagcgag gggtcacggg
     9841 ggttgatgcc gtcgatggct cggagctgcg gcgtccagat atcgttcatt tcgttgtaat
     9901 cgcagccctc gtacccgatg ccgtagccgt ccaggccaat gtgagtatgg gcatcgacga
     9961 agccggggta gacgtcgaga ccgtccgcgt cgaagatttc cgcgtccgcc ggggcggtca
    10021 gatcggtgcc gatgccggca attttgccgc ccgccagcag gatatcggcg atatacgccc
    10081 cgcggtggac cgcgtcatgg ataaggccgc cgcggatgag ggtggtcatg gttattcctc
    10141 cgttataccg taggggcgca ttctatatgc gcccgcagag tccgcggctg cggaagcgtc
    10201 cacgggcgga tatagaatcc gcccctacat tttattttct gcttacttac acattaaaat
    10261 ccaaacacgt ccgtaccgcc gtgtatggtc ttactttacc gtcggccaca gcgacgtggg
    10321 ccgggtgggt ggagtagcct ttcagtgcgg cggcatcggt gaaagtcgtg tccagcatca
    10381 ggtctgcatt ggcactgctg ggcagggcgt cggtgtagac atggacttcc agcagaccgg
    10441 ggatcttacc caccaaacct tccagcccgg ctttgatgtc tgccttgacg gcggccttct
    10501 ctgcgtcggg catatccttc agtttccaca aaatgacatg gcgtaccatt acttgttctc
    10561 ctcccagatg ggatgtttga gctgccactc gtcgtactcg ttcttctcga acaggtcacg
    10621 gttgtagaac ttgtccatga tggcccagaa ctccgtctcg gtgtagccgc agaaatcgca
    10681 gaaatcacgc acgcagagcg gatccagctt gccatcgcgt tccttgatga tggggatagc
    10741 ttcctcgcgg gtcatcatgc cgtagcggat gtagcgggca gtatagtcgg tggcggcggc
    10801 gtggccgaac ttggggtatt tcagccagga atgcaccagg tacgcgcggg agtcgatctg
    10861 gtcgaagttc tcggcgtggt gggtgcggtc ccactcgtgg gtcaggtcgt ggaagccgtg
    10921 ggccttggcg atctggtagt tcttgtagct gttccagggc acaaagtaac tcatgtagaa
    10981 cgggtcgagc ttggcgcgct cctcggcgct gggggccagc gtcaaggaca ggtccttctc
    11041 ggtcacgccg tcgcccagca gctcgtccat gggcatgccc acagcgacgc cgttctcgat
    11101 ctgatccatg gcggagtagg tctccttgtc ggcgttgccg ccgtactcaa agctgacgtt
    11161 ctcgccgtag cagagcatag gggtgttgaa cttggccgcc atgtgcagcg ggaaggtgta
    11221 gatcaggcgg tcaacgtacc aggtcggctt gccgtatttc tcaaagaatt tgcgcatcag
    11281 gaccttctgg accttgatgt taggcttgca ggagatgatg gtgcagccga actcctcgct
    11341 gatgttcttg aggttgtgga tacccgcctg cgtcatgggg aagttgtcct cgacgctgaa
    11401 caggatgggg ttcatgtgca tgacttcctt gagcatccag acctggaagt ggctgtcctt
    11461 gccgccggag acggcgacgg cacagtcata gccgccgggg ccgttcatgc cgcggtattt
    11521 gtcgcagtag gcctcaaact ccttgaagcg ggcgtcccag tcgacgtttt tgcggttctc
    11581 gtagtggcgg caggcgctgc acacgccctc ggcgtcaaag gtgataccgg gccgggtgtc
    11641 gggcatcgta cattttttgc agtaacgcat atgaaatctc cttaatttat atatagtatc
    11701 gggttcggtt gcaggggggc ggcgtcctcg acgccccgcg ggcggttgca agcatccgcc
    11761 cctatataac atggtgtctt gtcttattgt tttgcatccg ctgccgcctg ggccgctgcg
    11821 tgcaaggcgt ccgcatcggc gaaatcgtag atgacatagc ggccatcgtt gtacacctcg
    11881 gtgcagtcac cgtactggat tagcttggcg ttctctgccg ggccgtccgt ctcggtgtcg
    11941 atgagcagga aggggtgctc cgggtcggtg gtcaggtggc tcttgggctg cagccagttg
    12001 ggcacatcgt cgcggtccag cgattgcagc gtccagactt ccagcttgcc gctggtcaac
    12061 tcggtcatgg cgttgccgtt ccagaatgtg gaatagccct gggtatagcc gttgtccacc
    12121 agccattcgg cggcggtgtt cagccccttg tgggccaggt aggggtgttc ctgctcctgg
    12181 cgtaccgtgc tgacggcgca gagcgtaaag caggccgcca gccccacgcc cagcagctgc
    12241 cgggcaccca ggatgtggaa gtcctcggtc ttgatctcca ccgccatcac ggcgatggcc
    12301 accggcatat tcatgagcca gaagtagagg taatactcgt ggaagtaggt gtaggccacg
    12361 ccgcagaccg ccagcatggc gacgagcagc agcaccagca gcttgtcgtt gcgttccagc
    12421 ttgtccagcc gcagcagcag ccgcacgatg caaaagaaca tccagcagcc cagcagcagg
    12481 cccacggccg ccgcaatgcc gccgaagtgg aacacaccgg agccgttctc gtagccaaac
    12541 tcatggaaga agtccatcag gatgcggtcg agggtgaacc aatcctcgtc gcggttccag
    12601 acgatgaagt tgtaggattt gaagtcatac atccgggaca aaaccgcgtt gctgacgaag
    12661 taccccgccg cagcggccac cgccgtgacc agcgacgccg caagcaagcg aacttctttg
    12721 cggcagacgt ccagggcggc cttccagtcg gtcttgccgc agctgtgcag tgccagcacc
    12781 aacagaatgg ccgctgccag acagagcggc gcgtggaaca ccatgagctg cttgacgccg
    12841 ttcatgccgg ccaccgccgt gatgacgcaa gccagcaccc attgcagggc gcagtgcttt
    12901 ttatcggcgt tcaggctgcg cagcaccagc gccagtacca gcatataaaa gaacgtataa
    12961 acgagataat agccgccgta gatcgcgtag accaggtaat gctgtccgaa gggccacagc
    13021 agcgtgccga ccatccacag gcccgcgcgc cccagccccg cgcatttgac gaaaaagagc
    13081 atggccgccg cgaacagcgc cagcgtgatg gccatgccaa acgtgcgggc caggtgccag
    13141 tcattgggga aaatcagcag tcccaggcgg tagaaccatt gcaggttgac gactttcagc
    13201 tcggtggagt agaaccagtt atggctgatg atggttccct ctttgttgag caggtcggag
    13261 aggatcattt ccgaggcaag gtcggaatcg acgatccatt tgccgggcac cagctgatac
    13321 cacaggtcaa acaggtacgc tgccgccatc cacagccacg gcagcgcctg ccagaccttg
    13381 tgcagcgccc cgccctttgt ttgttgtttc atgcagtttc tcctaaatcg gatatgttca
    13441 gttaagataa ctataccgca ttttggggaa aatagcaagg gtgcgcgatg catttgtaga
    13501 ggccgcacat ctgtggcccg cgtgtgtagg gcgaatggtt tcttatggga aagctgcggg
    13561 ccgggcatgc ccggccccta caaacacaaa aggcggcggg ccaaacggcc cgccgcgggg
    13621 atgcgttgtt aaattattcc acaataatgc tcttgccggt catttccgcg gggacgggca
    13681 ggccgatata gggcagcatg gtgggggcga tgtctgccag gcagccgccc tcgcgcagct
    13741 tcacgtcgcc agcgccgatg accgcgaagg gcacgacatt ggtggtgtgc gcggtgaagg
    13801 gagaaccgtc ggggttcttc atcttctcgg cgttgccgtg gtcggcggtc aggaatacca
    13861 caccgcctgc gttcagtacg gcgtcggtga cttcgccgac gcacttgtca acagcctcaa
    13921 cggccttgac agcggcatca aacacgccgg tgtggccgac catatcgcag ttggcgaagt
    13981 tcaggatgat gacatcatac ttgccgctct cgatgcgggc cttgcactcg tcggcgacct
    14041 cataggcgct catctcgggc ttcaggtcgt aggtggcaac cttcgggctg gggatgacct
    14101 tgcgatcctc gccctcgcag ggctgctcga cgccgccgtt gaagaagaag gtcacatggg
    14161 catatttctc ggtctcggca atgcgcagct gggtcttgcc gttggcggcc aggtactcgc
    14221 ccagggtgtt cttcagctcg accggcggat aggcaacttc gcagttgggc atcgtggcgt
    14281 cgtactcggc catgcagacg tagttgacct gcttgcggcc gccgcggcgc tcaaagccgg
    14341 tgaaggcgtc atcgcagaag atgcgggtca tctggcgggc acggtcgggg cggaagttca
    14401 tgaagatgac ggtgtcgcca tcctcgacgc ggccgccctc gcaggtgaca aca
//
LOCUS       DBMDSQ010000023        14246 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_1334_length_14246_cov_2.217391, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000023 DBMDSQ010000000
VERSION     DBMDSQ010000023.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 14246)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 14246)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14246
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1334_length_14246_cov_2.217391"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(15..1547)
                     /locus_tag="ACZPAX_01710"
     CDS             complement(15..1547)
                     /locus_tag="ACZPAX_01710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1112647.1"
                     /translation="MRTKRTLLNMAYALGSSLLLLLLGLVTRRLLVDNFGPQITTASQ
                     VVSQLFNFFSIAEFGVGSVISYRLYEQIAAKNEEKISKYMSMYKWAYRVVGMVIAGLA
                     LIGAAVLRWIMPDVPAATAYTVYGLNVVSTLCSYFLITRRLMYTCTQQGYRCTQIDFC
                     CNVATSLAKIAVSLWFPNYALYFSVTIFFNVMANLIIARRFKKDFPYVHDVKVTVNDF
                     KELGIFHDLRYFLVHRLSNTIYGSSDTIVTSRMGGSTQTTFLGNYNTISTSATDLGNK
                     VMDSFAAAIGNIVYDKSAAANDHDKQVFWGMDLFSYLFGSFVATAYFCLFQPFMGAWM
                     GEKWLLPLSFVLVFCLNEYVGWNHRMLGSYRAVLGHFEEDQWFMVASATVNLALSFIL
                     FPLYDITGALIATVVAHCIMWAGRIRVVFRQYMCGGLGHYLAVQALHLVTLAACMGGS
                     YALCARMPGGWLGLAPRVLVVLVVPNLLNLAVYGWGKDAAYLRQYGAKVLRKLKRKGD
                     AA"
     gene            complement(1765..2754)
                     /locus_tag="ACZPAX_01715"
     CDS             complement(1765..2754)
                     /locus_tag="ACZPAX_01715"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR1112648.1"
                     /translation="MPRFSIIVPVYNVQNYLAACVKSVAEQAGERDWECILVDDGSTD
                     MSGTMCDAFAAEIPGMQVVHRKNGGLAAARNTGLKHAQGEWVLFLDSDDAMAPGLLAA
                     LRTELAAHPEYDWFIGKHLEWQPDGSLTEHAGLHLVPGMFDSSDYAARLKALYGAGHW
                     SVWKYCIRRSFLAESGVRFLPDCVWAEDWPFDLELLIKCERLYFLDVVFTHYRVGRQG
                     SLLTDAKNLPKRFKGLAAAQRRLAKLSASGAVDAETYAVMENAAADVFWPQARTASVK
                     DAAIRKACLPYIQQLRPLYACGTEVKTRRDWRAFQKLMQTFGPKFTLWAASRR"
     gene            complement(2926..3876)
                     /locus_tag="ACZPAX_01720"
     CDS             complement(2926..3876)
                     /locus_tag="ACZPAX_01720"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013681.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008417 - fucosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043413 - obsolete macromolecule
                     glycosylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-1,2-fucosyltransferase"
                     /protein_id="HIR1112649.1"
                     /translation="MIYAELAGGLGNQMFIYAFARALGLRCGEGVTLLDRQDWRDGAP
                     AHTVCALGALNISPEVEILADSGFAKAHLPRQNTAKALMIKYEQRRGLLARDWHGFEA
                     TAAPLLNALGLHFATDGYTPARRGKSKDFLAWGYFQSEKYFDDFADVVKTELRSKAVP
                     VGEYADRIRAAAWPVCVHLRRGDYQKPENAILQVCTPAYYARAAARVKAARPEAELFV
                     FSDDIAWAQEHLDTAGLPAVFMPQGAAVDDLALMQLCHGFVVSNSTYSWWAQYLAEAP
                     DKQVWAPDRWYAHTKDSALYLDGWKLIKASPLGEAGREAD"
     gene            complement(3873..4979)
                     /locus_tag="ACZPAX_01725"
     CDS             complement(3873..4979)
                     /locus_tag="ACZPAX_01725"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR1112650.1"
                     /translation="MRILMISNHLGVQSGVQRYVQNLLLNLDTTKYHIDLFVGQCPAD
                     QASTAPALEARGVHIIAVPDNKKDRIRALAAHLRTHKDYDIIHYHTASKIGAPVCGMM
                     RLLCPRAKIVVHSHIVYPPLTLTWRAAHGVYQLFADYFLGCGVAAGRFVFGDHIDEKP
                     NFSVACNAVDAGRFHPDAAARAATRASYGIADTDRLAGFVGRLNHQKNPLFLMEVFAA
                     MAAQDVRWKLLLVGTGEKEPEIRAAAEAHGLADRVIFAGVQSDVPAFMNAFDLFLLPS
                     NFEGSPVTLVEAQGCGVPCLASTNVPDDGSVTELVHFLPLAAPLTEWAAKADEIAVGG
                     DHADHWQTLAAAGYELKTAAHRMEQLYDKLGGRA"
     gene            complement(4992..5900)
                     /locus_tag="ACZPAX_01730"
     CDS             complement(4992..5900)
                     /locus_tag="ACZPAX_01730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1112651.1"
                     /translation="MDNDNILLQKAADIYRGLRFKMKYRGVDILPAGAGNDLARELLE
                     SGQPFLFGRCGATEMRTVADYLQNGGKNYADRTREDIRNLSGVFPTDDATLEKFCRLY
                     VKCAQNAELLALWNVGAEREVIRGCDATRFTELRALEPYYHAKPWSAALAGRRVLVVH
                     PFRKTILAQYARRAQLFPGKNVLPEFASLTVVQAVQGLGGQDTGYASWFDALAAMERE
                     MDAADYDVAIVGAGAYGLPLAAHARDTGHAAIQMSGATQLLFGIKGKRWDSHPIIGKL
                     YNDAWVRPDETEGITHKEKVEGGSYW"
     gene            complement(5893..7005)
                     /locus_tag="ACZPAX_01735"
     CDS             complement(5893..7005)
                     /locus_tag="ACZPAX_01735"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR1112652.1"
                     /translation="MKTIVIPQFYRGPSGQKGLYNRQEVGLARAFAALGCRAVVLYPE
                     PGAKAPKVETPEPNVKICYLPAVAFGTQALYKSWQILLDEQADAVHVMGDNSLGVPGL
                     YRFCEKHGIVFYSQLGALKSTSNHAIVRGVMNLLLHRNLTVYRKTPTYAKTPAVAAEL
                     ESLGVPCRGLMPVGLDTAIIPTIPGSKAMIRRELNINEQVKVLAFVGRLDAYKQPLDL
                     IPLLQAAPDWYAVIIGQGAQSDELARRLTEAGLMGRCRLIPQLPNAQVHIYYHACNAF
                     VNLNENEIFGMSLLEAMYAGCPPVARHAPGPDLIIENGVSGYLCDTVAEMAAALDKTT
                     DAMGHAAQNRVNEHFLWQNSAELALTLLPKKGKTNG"
     gene            complement(7006..8013)
                     /locus_tag="ACZPAX_01740"
     CDS             complement(7006..8013)
                     /locus_tag="ACZPAX_01740"
                     /inference="COORDINATES: protein motif:HMM:NF048899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine inositolphosphorylceramide
                     transferase family protein"
                     /protein_id="HIR1112653.1"
                     /translation="MNQFYRALHMPPKRLLRKVMGEKEVYTIAVRPRPTAEGTEPLPA
                     LGAGPYTMLPGREGWWFADPLLCHRGGKRWLFAEAMDLSTGKGRLEVCELHDDGSIDG
                     WRVALEEDFHLSFPMVFDWNGEVWMIPETGHDHSLRLYRCRVFPTKWELVQRFATDEE
                     LCDAILVNRRAEALTILCSETKPDNQLYVRYRRYTLCHAVQETAALPLAEADTLPAAA
                     PAGPFELLPDETYNLQHRDFDFVSRNAGPLFVLGEQVIHPTQVSTSVDYGVYLQFLAR
                     RGSSEVPLCAATPQNVEITGLNAADVIGVHTYCRDDVLEVIDARYLTRIPKPAKPEAE
                     V"
     gene            complement(8015..9184)
                     /locus_tag="ACZPAX_01745"
     CDS             complement(8015..9184)
                     /locus_tag="ACZPAX_01745"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR1112654.1"
                     /translation="MKILLVHCHYRLPGGEDAVFAAERELLERHGHTVLVYERSNEEA
                     AHGAAKALLPLHAVWNRRAAREVKDLIKREGVEAVHIHNTLLLLSPAVVRAAKSCGVP
                     VVQTLHNFRLFCPNGILLRDGQVCEECPHHGLHCALKHSCYRGSKAQTLVVVAAYWLH
                     RVLGTWRGVTMAAPTEFDREKLLEFNKIYPMFDANRLVVKPNPVCVPNGPVTPWDERK
                     RQFVFAGRLEELKGIRTVIEAWRILDKNAPELLVAGDGPLADWAKAQKLPKVRFLGQL
                     PRNELHRIIGESRAVVGASLCYESFALVPAEAHALGTPVLASGIGNMGASVRPGFDGL
                     CFAPGDAAALAGAVRAMSAMQFDCAAIAAATRRRFGEEENYKTLMRLYGRSAAEE"
     gene            complement(9376..10167)
                     /locus_tag="ACZPAX_01750"
     CDS             complement(9376..10167)
                     /locus_tag="ACZPAX_01750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HIR1112655.1"
                     /translation="MGLFHQNFDKPGPGVSPDAPRKKGAARFFEILGRDFSTIWMAGL
                     LALISSLPFALLLWFAVSTHSLIPMLAAGILGGMIAAPQIVGLNDTILRSLRDEPGFW
                     WATYRCAWKRNVKASLLPGAVCGLLLAMEIFTAFHLDISQSVVPAVAVLVALILLAGI
                     AQYIYAQVALVEVSFGGLLKNALMLFLGYLPRSALGVLWQGIYWAVVALFWPVSSFAV
                     ILCSLWLPCLLNLMAIYPALDKSFDLERTIKAMRDAQLNSENEDK"
     gene            complement(10202..10885)
                     /locus_tag="ACZPAX_01755"
     CDS             complement(10202..10885)
                     /locus_tag="ACZPAX_01755"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005929046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HIR1112656.1"
                     /translation="MGVEILDNGTRVLTAPGAMFGTDALLLGRFAQPRRDERALDLCS
                     GCGIVALLWHDAGHRGPCTALEFDPAASALCGQAAAENGAEHITPVCADLRQFCTAGP
                     EQGQYDFAACNPPYFAAGPRSPDKRRAEARHTDSCTTADAVDCARRALRDGGRFLLCQ
                     RPEQLAEVLSTLRAARLEPKRLAFVKNRPDAAPWLFLVEAQKGRKTGLRVEPDVLITS
                     GAAMYGPRE"
     gene            complement(10886..11425)
                     /locus_tag="ACZPAX_01760"
     CDS             complement(10886..11425)
                     /locus_tag="ACZPAX_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013484841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HIR1112657.1"
                     /translation="MAHTETMLESKEIFNGRVIRVTVDKVQLEDGTTSTREIVHHHGG
                     ACILPVDADGNVTMVRQFRYAFGEEIWELPAGKLEAGEDPFEAAKRELSEECGLTADN
                     FIDLGVVYATVGYDSEKIYLWAATGLHEVKQHLDAGEFLDVVKMPFDKALGLVMDGTI
                     KDSKTQVALLKYAQLRDKA"
     gene            complement(11458..12906)
                     /locus_tag="ACZPAX_01765"
     CDS             complement(11458..12906)
                     /locus_tag="ACZPAX_01765"
                     /inference="COORDINATES: protein motif:HMM:NF024949.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1112658.1"
                     /translation="MTELIQSLKAAVARYPGTMAELARRAAIDRSTLYKILGGQRTPR
                     REQLERLTDALELTEEEKTTLLVQYKQRGRGTDPKMRAALNQLLEAAFRVEDYTRREG
                     NITNEAARQMSVAGQYVEGTRAVTAAAAALVAEHMLSGDTRPLLLSPFTNPVLDRVLV
                     NRFSAPDGQTASLSQLLIFVEGSDISREKAGNIATLTRTLPLLFLPKLNYDARVVRCV
                     MSEPSPGTLMPVYLLLPTAALMMDRLGQKGYIITDREAVENLRLAFSRKYIDAVPVLK
                     LATGTHGFMESVQLYSKLFAETRKCCMVRYQPPFSLFADEKIALRVVRQDAAVQPCLP
                     ALLDYLNTWNQQTPDLYFCEEGLLQFVRTGQMFDLPPALYEPPDVETRRELLLRLRRA
                     AASDKQTLRIVESAQLPLTPSMSVNAFRGRGVVFCQAVLEPGKMYCREYLMEDPQMTE
                     AVLNYLEEGKTGDRVRSQKYTVDFIDYCLRLL"
     gene            13132..14118
                     /locus_tag="ACZPAX_01770"
     CDS             13132..14118
                     /locus_tag="ACZPAX_01770"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012770.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR1112659.1"
                     /translation="MKKHPLLTAAAAAGTVVLGAAYGIYRVAFYQKPTTAVPSPADMP
                     TREVYRKAMCPDADALARDTFTPVHITAQDGTPLAARYYHHNDGAPLAIIFHGYRGYA
                     VRDGLGGYTLCTKLGYNVLLPDQRAHGYSGGHTITMGVKERYDARDWAVWARSHFGPE
                     VPIFLMGVSMGAATVLLAAGLNLPDNVCGIVADCGYTSPREITRKCLPEYLPGFPLGP
                     TYAIGRLGAILFGHFDPEDADCTDAAAHSKVPIFFVHGDADGFVPYEMGRRNYEVCRA
                     KGKRFLTVHGAEHAVSYYHDNTAYTKECTDFLQDCLTRRHFAWPKEAPDDAR"
BASE COUNT         2638 a         4757 c         4176 g         2675 t
ORIGIN      
        1 catcccgatt tttgtcatgc agcatccccc tttcgtttca gcttgcgcag caccttcgcg
       61 ccgtactggc gcaggtaggc ggcgtctttg ccccagccgt agacggccag gttcagcagg
      121 ttgggcacca ccagcaccac cagcacccgg ggtgccaggc ccagccagcc gccgggcatt
      181 ctggcgcaca gcgcgtagct gccgcccatg caggctgcca gcgtgaccag atgcagcgcc
      241 tgcacggcca ggtaatgccc cagcccgccg cacatatact ggcggaacac gacccggatg
      301 cgcccggccc acatgatgca gtgggccacc accgtggcga tcagtgcgcc ggtgatatca
      361 tacagcggga acagaataaa gctcagcgcc aggttcaccg tggccgacgc caccatgaac
      421 cactggtcct cctcaaaatg gccaagcacc gcgcggtagg accccagcat ccggtggttc
      481 cagccgacgt actcattcag gcagaacacc agcacaaagc tcaggggcag cagccatttt
      541 tcgcccatcc acgcgcccat aaacggctgg aacaggcaga aatacgcggt tgccacaaag
      601 ctgccgaaca ggtagctgaa cagatccatg ccccagaaca cctgcttgtc gtggtcgttg
      661 gcggcggcgg atttgtcgta gacaatgttg ccgatggccg ccgcaaagct gtccatgacc
      721 ttgttgccca ggtcggtggc ggaggtggaa atggtattgt agttgcccaa aaacgtcgtc
      781 tgggtcgagc cgcccatgcg ggacgtcacg atggtatccg acgagccgta gatcgtgttg
      841 gagagacggt gtaccaaaaa ataccgcaaa tcgtggaaaa tacccagctc tttaaagtcg
      901 ttgaccgtga cttttacgtc atgcacatag gggaagtcct ttttgaaccg ccgcgcaatg
      961 atcaggtttg ccatgacgtt aaagaagatc gtcacgctga aatacagcgc atagttcggg
     1021 aaccacagcg agacggcaat tttcgccaga ctcgtcgcca cgttgcagca aaagtcaatc
     1081 tgggtgcagc ggtagccctg ctgggtgcag gtgtacatca ggcggcgggt tatcaaaaag
     1141 tagctgcaca gcgtcgaaac cacgttcagc ccgtacacgg tgtaggccgt ggcggcgggc
     1201 acatcgggca tgatccaccg cagcacagcc gcgccgatga gtgccagccc cgcaatgacc
     1261 atgccgacga cgcggtacgc ccatttgtac atcgacatgt acttgctgat tttttcctca
     1321 tttttcgcgg cgatctgctc atacagccgg tagctgataa cgctgccgac gccgaactct
     1381 gcgatcgaga aaaagttgaa cagctggctg acgacctggg acgccgtggt gatctgcgga
     1441 ccgaagttat cgaccagcag ccgccgtgtg accagcccca acagcaacag cagcaggctg
     1501 gagccaaggg cgtaagccat atttaataag gtacgtttgg tacgcaaggc agttcctttc
     1561 acaaaagcct tcccccgagg gggaaggtgg cccggagggc cggatgaggg gagagtatga
     1621 gttgtaagcc gttaatgggc ggtgatggcg aggttagccc tcatcagtcg cctacggcga
     1681 cagcttcagt ctccgcgtca agagccgcct acggcggtta cgcttcgaca cgcgcctgcg
     1741 ggcgcagccc cttgagggaa gcctttagcg gcggctcgcc gcccacaggg taaatttcgg
     1801 gccgaaggtc tgcatcagct tttggaacgc ccgccagtcc cggcgggtct ttacctcggt
     1861 gccgcaggca tacaatggcc gcagctgctg gatgtagggc aggcaagcct tgcggatggc
     1921 ggcatccttc accgacgccg tgcgggcctg cggccagaac acatcggccg ccgcgttctc
     1981 catgacggca taggtctcgg catccaccgc gccgctggcc gagagttttg ccagccgcct
     2041 ctgggccgcc gccagcccct tgaaccgctt gggcaggttt ttcgcatcag tcagcaggct
     2101 gccctgccgc cccacgcggt aatgggtgaa caccacatcc aaaaagtaca gccgttcgca
     2161 ctttatcagc aattccaaat caaaaggcca gtcctcggcc caaacgcagt caggcaaaaa
     2221 cctcacgccg ctttcggcca aaaacgaccg ccggatgcag tatttccaca ccgaccagtg
     2281 ccccgcgccg tacagcgctt tcaatcgggc ggcgtagtcg ctgctgtcaa acatcccggg
     2341 caccagatgc agccccgcgt gttcggtcag gctgccgtcg ggctgccatt ccagatgctt
     2401 gccgatgaac caatcgtact ccgggtgggc ggccagctcg gtgcgcagcg ccgccagcag
     2461 cccgggggcc atggcgtcgt cgctgtccaa aaacagcacc cactcgccct gggcgtgctt
     2521 tagtccggtg ttgcgggcag cggccaggcc tccgtttttc cggtggacga cctgcatacc
     2581 ggggatctcg gcggcgaacg cgtcgcacat tgtcccgctc atatcggtgg atccatcgtc
     2641 cacaaggatg cactcccaat cgcgctcccc cgcctgctcc gccacgctct tgacacacgc
     2701 cgcgaggtaa ttctgcacat tatacaccgg tacaataata gaaaatcgcg gcataacgtt
     2761 ctccttctct atgccttcct ccgtcggggg aaggtggccc cgcagggccg gatgaggggc
     2821 agccttgccc cggcagcccc cttgccttgt tgtaggggcc gatgctcaca tcggcccgcg
     2881 cagcactgtc ccaacagcgg ccaaatgcgg ttcacgcgcc cctcatcagt ccgcttcgcg
     2941 gccagcttcc cccaaggggg aagcctttat aagtttccac ccatccagat acagcgcact
     3001 atccttcgtg tgcgcgtacc acctgtccgg ggcccagacc tgcttatccg gggcctcagc
     3061 caggtattgc gcccaccagc tgtatgtgga gttgctcacc acaaacccat ggcagagctg
     3121 catcagcgcc aggtcgtcca ccgccgcgcc ctggggcata aacactgcgg gcaaccccgc
     3181 cgtgtccaga tgctcctgcg cccaggcgat atcgtccgag aacacgaaca gctccgcctc
     3241 ggggcgggcc gctttcaccc gcgccgctgc ccgggcgtaa tacgcaggcg tgcagacctg
     3301 caagatggcg ttctccggct tctggtagtc accgcgccgc agatgtacgc agaccggcca
     3361 ggccgcggcg cgtatcctgt cggcgtactc ccccaccggg actgctttgc tgcgcagctc
     3421 ggtttttaca acatcagcaa aatcgtcaaa atatttctca ctctgaaagt acccccacgc
     3481 gaggaaatct ttactttttc cccgccgcgc cggcgtgtag ccgtcggtgg caaagtgcag
     3541 gcccagcgcg ttcagcagcg gtgcggcggt ggcctcaaag ccgtgccaat cccgggccag
     3601 caggccgcgg cgctgctcat actttatcat cagggccttg gccgtgttct gccggggcag
     3661 atgcgccttg gcaaagccgg aatcggccag aatttctacc tcggggctga tgttcagtgc
     3721 ccccagtgcg cagaccgtgt gggcgggcgc accgtcgcgc cagtcctgcc ggtccagcag
     3781 ggtcacaccc tcgccgcagc gcaggcccag cgcccgcgca aaggcgtaaa tgaacatctg
     3841 attgcccagg cccccggcca gctccgcgta aatcatgctc gccctcccag cttgtcgtac
     3901 agctgctcca tgcggtgggc ggcggttttc agctcgtagc ctgccgccgc cagcgtctgc
     3961 cagtggtcgg cgtggtcgcc gcccacggca atttcatcag ccttggccgc ccactcggtc
     4021 aggggggcgg ccagcggcag aaaatgcacc agctccgtca ccgagccgtc atccggcaca
     4081 ttggtcgatg ccagacacgg cacgccgcag ccctgcgcct ctaccagcgt gaccgggctg
     4141 ccctcaaaat tgctgggcag caaaaacagg tcaaaagcgt tcataaaggc gggcacatcg
     4201 ctctgcacgc ccgcaaaaat cacgcggtcg gccagaccgt gggcctcggc ggcggcgcgt
     4261 atttccggct ctttctcgcc ggtgccgaca agcagcagtt tccaccgcac atcctgcgcg
     4321 gccatagccg caaaaacctc catcaaaaac agcgggtttt tctggtggtt cagccgcccc
     4381 acaaacccgg ccaggcggtc ggtgtcggca atgccgtagc tggcccgcgt ggcagcccgc
     4441 gcggcggcgt ccgggtggaa ccgcccggca tccacggcgt tgcaggccac ggaaaagttt
     4501 ggtttttcgt cgatatggtc gccaaacaca aatcgccccg ccgccacgcc gcagccgagg
     4561 aaataatccg caaacagctg gtagacgccg tgggcggccc gccaggtcag cgtcagcggc
     4621 gggtagacaa tgtggctgtg taccacgatc ttggcccggg ggcagagcag ccgcatcatg
     4681 ccgcagaccg gtgcgccgat cttgctggcc gtgtggtagt ggatgatatc gtagtccttg
     4741 tgggtgcgca ggtgcgccgc cagtgcccgg atgcggtcct ttttattgtc cggcaccgca
     4801 atgatatgca cgccgcgcgc ttccagcgcg ggggcggtgc tggcctggtc cgccgggcac
     4861 tgccccacga acaggtcgat atggtatttc gttgtatcga ggttcagcag cagattttgc
     4921 acataccgct gcaccccgct ctgcacccca aggtggttgc tgatcattaa gatacgcata
     4981 aagttctccg attaccagta gctcccgcct tcgacctttt ctttatgggt gatgccctcc
     5041 gtctcgtcgg ggcgcaccca ggcatcgttg tacagcttgc caatgatggg gtggctgtcc
     5101 caccgtttgc ccttgatgcc aaacaaaagc tgcgtggcac cgctcatttg gatggcggcg
     5161 tggccggtgt cccgggcgtg ggccgccagc ggcaggccgt aggctcccgc gccgacgatg
     5221 gccacatcgt agtcggcggc gtccatctcg cgctccatgg cggccagggc gtcaaaccag
     5281 ctggcatagc cggtgtcctg cccacccagg ccctgcacgg cctgcaccac ggtcagcgac
     5341 gcaaactccg gcaggacatt cttgccgggg aacagctgcg cccgccgggc atactgcgct
     5401 aaaatcgtct tgcggaaggg atgcaccacc aacacccgcc tgcccgccag tgccgcgctc
     5461 cacggcttgg cgtggtagta gggttccagt gcgcgcagtt cggtaaagcg ggtggcatcg
     5521 cagccgcgga tgacctcccg ctcggccccc acgttccaca gggccagcag ctccgcattc
     5581 tgggcacatt tgacgtagag acggcagaat ttttccagcg tcgcatcatc cgtggggaac
     5641 acgccagaca aattgcggat atcctcccgc gtgcggtcgg cataattttt gccgccgttc
     5701 tgcaaatagt cggccacggt gcgcatttcg gtcgcgccgc agcggccaaa caaaaacggc
     5761 tggccgcttt ccagcagttc ccgcgccagg tcgttgccgg ccccggcggg taagatatcc
     5821 acgccgcggt atttcatctt gaaccgcaga ccgcggtaaa tgtcggcggc cttttgcagc
     5881 agaatgttgt cgttatccat tggttttccc ctttttcggc agcagggtca gggccagttc
     5941 ggcgctgttc tgccacaaaa agtgttcgtt cacgcggttc tgcgccgcat ggcccatggc
     6001 gtcggtcgtc ttgtccagcg cggcggccat ctcagcgaca gtatcacaca gatacccgct
     6061 gacgccgttt tcaataatta aatccggccc cggcgcatgg cgggccaccg gcgggcagcc
     6121 cgcgtacata gcctccaaca ggctcatgcc gaaaatctcg ttttcgttca ggttcacaaa
     6181 agcgttgcag gcatgataat aaatatgcac ctgcgcgttg ggcagctggg ggatcaagcg
     6241 gcagcggccc atcaacccgg cctcggtcag gcggcgtgcc agctcatcgc tctgcgcccc
     6301 ctgcccaata atgactgcgt accagtcggg cgcggcctgc agcagcggta ttaggtcaag
     6361 cggctgcttg taggcgtcca gccgccccac aaaggccagc accttgacct gctcgttgat
     6421 gttcagctcc cggcgtatca tggccttgct gcccggtatc gtcgggatga tggccgtgtc
     6481 cagccccacc ggcatcagcc cgcggcaggg cacgcccagg ctttccagct cggcggccac
     6541 agccggggtc ttggcatagg tgggtgtctt gcggtacacc gttaaatttc tgtgcagcag
     6601 caggttcatc accccgcgca caatagcgtg gttggaggta cttttcaacg cccccagctg
     6661 gctgtaaaac acgatgccgt gcttctcgca aaagcggtac agccccggca cgcccaggga
     6721 gttatccccc atgacgtgta cggcatcggc ctgttcgtcc agcaagatct gccagctttt
     6781 gtataatgcc tgggtgccga aggccacggc gggcaggtag cagattttca cgttcggctc
     6841 gggtgtctcc accttgggcg cttttgcgcc cggttcgggg tagagcacca cggcacggca
     6901 gcccagcgcg gcaaacgccc gggccaggcc aacctcctgt cggttgtaca gccctttctg
     6961 gccggagggg ccgcggtaaa actgcggtat gacaatggtt ttcatttaca cttctgcctc
     7021 cggctttgcg ggcttgggga tgcgcgtcag ataccgcgca tcgatgactt ccagcacatc
     7081 atcccggcaa taggtgtgta cgccgatgac atccgccgcg ttcagtccgg tgatttccac
     7141 attctggggc gtcgccgcgc agagcggcac ctcagagctg ccgcgccgcg cgagaaattg
     7201 caggtaaacg ccgtagtcca cgctggtgct gacctgcgtg gggtggatga cctgctcgcc
     7261 cagcacaaac agcggccctg cattgcggct gacaaaatca aaatctcggt gctgcaaatt
     7321 gtatgtctcg tcgggcaaaa gttcaaaggg gcctgcgggc gcggcagcgg gcagggtgtc
     7381 tgcctcagcc aacggcaggg cggcagtctc ctgcacggca tggcacagcg tgtagcggcg
     7441 gtagcgcaca tacagctggt tgtcgggctt tgtctcgctg cacaaaatcg tcagtgcctc
     7501 ggctcgacga ttcaccaaaa tcgcgtcgca cagttcctca tcggtggcaa accgctggac
     7561 cagctcccat tttgtgggga acacccggca gcggtaaagc cgcaggctgt ggtcgtgccc
     7621 ggtctcgggg atcatccaga cctcgccgtt ccagtcaaac accatcggga aggaaaggtg
     7681 gaaatcctcc tccagtgcca cacgccagcc gtcaatgctg ccgtcgtcgt gcagttcgca
     7741 cacttccagc cggcccttgc cggtggaaag atccatcgcc tcggcaaaca gccagcgctt
     7801 gccgccccgg tggcagagca gcgggtcggc gaaccaccag ccctcgcggc cgggcagcat
     7861 cgtgtagggg ccggcaccca gggcgggcag cggctcggtg ccctcggccg tggggcgggg
     7921 gcgcactgca atcgtataca cttctttttc gcccatcact ttgcgcagca gccgtttcgg
     7981 cggcatatgc agggcgcggt aaaactggtt catgtcattc ctcggcggca ctgcggccgt
     8041 acaatctcat caacgtcttg taattttctt cctcgccaaa gcggcggcgg gtggcggcgg
     8101 cgatggccgc gcagtcaaac tgcatggcac tcatggcccg cacggccccg gccagcgcgg
     8161 cggcatcccc cggcgcaaaa cacaggccgt caaacccggg ccgcaccgac gcccccatat
     8221 tgccgatgcc gcttgccagc accggcgtac ccagggcgtg ggcctcggcg ggcaccaggg
     8281 caaagctctc atagcacaag ctggcaccca ccacagcgcg gctctcgccg atgatgcggt
     8341 gcagctcgtt ccggggcagc tggcccagaa aacgcacctt cggcagcttc tgcgctttcg
     8401 cccagtcggc cagcgggccg tcgcccgcca ccagcagttc cggcgcgttt ttatcgagga
     8461 tacgccacgc ttcgataacg gtgcggatgc ccttcaattc ttccagccgc ccggcaaaca
     8521 caaactgccg tttgcgctcg tcccagggcg tcaccgggcc gttcggcacg cagaccgggt
     8581 tcggcttgac gaccaggcgg tttgcgtcga acatcgggta gattttgttg aattccagca
     8641 gcttttcgcg gtcgaactcg gtgggggcgg ccatcgtcac gccgcgccag gtccccagga
     8701 cccggtgcag ccagtaggct gccacgacca ccagcgtctg cgccttgctg ccccggtagc
     8761 agctgtgctt caaagcgcag tgcaggccgt ggtgggggca ctcctcgcag acctggccgt
     8821 cccgcagtaa aatcccgttg gggcagaaca ggcgaaaatt gtgcagcgtc tgcaccaccg
     8881 gcacgccgca gcttttggcg gcccgcacca cagcggggct gagcagcaag agcgtgttgt
     8941 ggatatgcac ggcctcgacg ccctcgcgtt ttatcaggtc tttcacctcg cgggcagcgc
     9001 gacggttcca gacggcgtgc agcggcagca gcgccttggc cgcgccgtgg gcggcttcct
     9061 cgttgctgcg ctcatacacc agcacggtat ggccgtggcg ttccagcaat tcccgctccg
     9121 ccgcaaaaac ggcatcctcg ccgccgggca gccggtaatg gcagtgtacc agcagaattt
     9181 tcacgctgtc gcctcctttt ccgcataata agcctattat gtcattatac cctttttata
     9241 aagaaggtgc aagatgttcc cgccgctttc gcagcccgcc gtccatgtag gggctgggca
     9301 cgcccggccc ccagccttcc cataaccgcc cctacaaaca aaggggaacg cgaccccgcg
     9361 ttccccttgc aatttttatt tgtcctcatt ctcgctattc agctgggcgt cccgcatggc
     9421 cttgatggtt ctctccaaat caaagctttt atccagcgcg gggtaaatcg ccatcaagtt
     9481 caaaaggcag ggcagccaca ggctgcacag gatcaccgca aagctgctga caggccagaa
     9541 cagcgccacc actgcccagt aaatgccctg ccacagaaca cccagcgcac tgcggggcag
     9601 gtagcccaaa aacagcatca gcgcgttttt cagcagaccg ccaaagctga cctcgaccag
     9661 cgccacctgg gcatagatgt actgtgcaat gcccgccagc agaatcagcg ccaccagcac
     9721 ggccaccgca ggcacaacgc tctgggagat atccaggtgg aatgccgtga aaatttccat
     9781 cgccagcagc agcccgcaga cagcgcccgg cagcagcgac gccttgacgt tgcgcttcca
     9841 ggcgcagcgg taggtcgccc accagaagcc cggctcatcg cgcaggctgc gcagaatcgt
     9901 gtcgttcagg ccgacgatct gcggtgcggc gatcatgccg cccagaatgc ccgccgccag
     9961 catcgggatc agggagtggg tggacacggc gaaccacagc agcagcgcga agggcaggct
    10021 gctgataagc gccagcagac ccgccatcca tatggtggaa aagtcgcggc ccagaatctc
    10081 aaagaagcgg gcagcaccct tttttcgcgg tgcatcgggc gaaacgcccg gccccggttt
    10141 gtcaaaattc tgatgaaaaa gtcccatgac ggtattctcc tagttttgta attttttagt
    10201 gttattctcg cggcccatac atcgccgcac cgctcgttat cagcacatcc ggctccacgc
    10261 gcaacccggt cttgcggccc ttctgtgcct cgaccaaaaa cagccagggc gcggcgtcgg
    10321 ggcggttctt cacaaaggcc agccgcttgg gttccagccg cgcggcacgc agggtgctca
    10381 atacctcggc cagctgctcg ggccgctggc acagcaggaa ccgtcccccg tcccgcaagg
    10441 cgcggcgggc gcagtccacg gcgtctgccg tcgtgcagct gtcggtgtgg cgggcctcgg
    10501 cccggcgctt gtcggggctg cgcggccccg ccgcaaagta gggcgggttg caggctgcaa
    10561 aatcatactg cccctgctcc ggccctgccg tgcagaactg ccgtaaatcc gcgcagaccg
    10621 gcgtgatatg ctcagcgccg ttctccgcag ccgcctggcc gcagagtgcg cttgccgccg
    10681 ggtcaaattc cagcgccgtg catggtcccc ggtgcccggc atcgtgccac agcagcgcca
    10741 caatgccgca cccgctgcac aggtccagcg cccgctcgtc ccgccggggc tgggcgaacc
    10801 gccccagcag cagcgcgtcg gtgccaaaca tggctcccgg cgcggtcagt acgcgggtgc
    10861 cgttgtccag tatttcaacg cccatttacg ctttatcgcg cagctgcgcg tatttcagca
    10921 gggcgacctg ggtcttgctg tccttgatgg taccgtccat caccaggccc agggccttgt
    10981 cgaacggcat cttcaccaca tccaagaact ccccggcgtc caggtgctgc ttgacctcgt
    11041 gcaggccggt ggcagcccac aggtagattt tttcgctgtc atagcccacg gtggcgtaga
    11101 cgacacccag gtcgatgaag ttgtccgccg tcaggccgca ctcctcactc agttcgcgct
    11161 tggcggcctc aaaggggtcc tcgcccgctt ccagtttgcc ggcgggcagt tcccagattt
    11221 cctcgccaaa ggcatagcgg aactggcgca ccatcgtcac gtttccatct gcatccaccg
    11281 gcaggatgca cgccccgccg tggtgatgga cgatctctcg ggtgctggtg gtgccgtcct
    11341 ccagctgcac cttatccacc gtcacgcgga tgacccggcc gttgaatatc tccttgcttt
    11401 ccagcatagt ctctgtatgt gccatggctt gtctcctttt ttgcatcttt cctaatctta
    11461 caacaaccgc aggcaataat caataaaatc caccgtatat ttttggctgc gcacccggtc
    11521 gccggttttg ccctcctcca gataattcag cactgcctcg gtcatctggg gatcctccat
    11581 cagatattcg cggcagtaca ttttgcccgg ttccagcaca gcctggcaga acaccacccc
    11641 gcgcccgcgg aaagcgttca cgctcatcga cggtgtcagc ggcagctggg ccgattccac
    11701 aatgcgcagc gtctgcttgt cggaggccgc cgcccggcgc agccgcagca gcagctcccg
    11761 gcgcgtctcg acgtccggcg gttcgtacag agccgggggc aggtcaaaca tctgcccggt
    11821 gcgcacaaac tgcaaaagtc cttcctcgca gaaatacagg tccggcgtct gctggttcca
    11881 ggtgttcagg taatccagca gcgcgggcag gcagggctgc acggctgcgt cctgccgcac
    11941 gacccgcagc gcgatctttt catcggcaaa caggctgaac ggcggctggt agcgcaccat
    12001 gcagcacttg cgcgtctcgg caaacagttt gctgtacaac tgcacggact ccataaaacc
    12061 gtgcgtcccg gtcgccagtt tcagcaccgg cactgcgtcg atgtactttc ggctgaacgc
    12121 caggcgcagg ttttccacgg cctcccggtc ggtgatgata tatcctttct gccccaagcg
    12181 gtccatcatc aaggcggccg tgggcagcag cagatacacc ggcatcagcg taccggggct
    12241 gggttcgctc atcacacacc gtacaacgcg ggcatcgtag ttgagcttgg gcagaaacaa
    12301 gagcggcagc gtgcgggtca gcgtggcgat attcccggct ttctcccggg aaatgtcgct
    12361 tccctcgaca aaaatcagca gctgggacag cgacgccgtc tgcccgtcgg gcgcggagaa
    12421 gcggttcacc agcacccggt ccagcaccgg gttcgtgaat ggcgagagca gcagcggccg
    12481 cgtatcaccg gatagcatat gctcggccac cagcgccgct gcggcagccg tcaccgcgcg
    12541 ggtaccctcc acatactgtc ctgcaacgct catctgccgc gcggcctcgt tggtgatgtt
    12601 tccctcgcgc cgggtgtaat cctcgacccg gaacgccgct tccagcagtt ggttcaacgc
    12661 agcgcgcatc ttggggtccg tgccgcggcc acgctgcttg tactgcacca gcagggtggt
    12721 tttttcttcc tcggtcaatt ccagtgcgtc ggtcaggcgc tccagctgtt cgcgccgagg
    12781 cgtgcgctgc ccgccgagta tcttatataa agtagagcgg tcgatggccg cccggcgcgc
    12841 cagttccgcc atcgtgcccg gataccgcgc caccgccgcc ttcaggcttt ggatcagttc
    12901 agtcataggt gggtcctttt tgttgcatcg tcaatccgtg tatagcaggg gtcgatgctc
    12961 gtcatcgccc tgcccctgca cttctacccg gaaagcattt tcttctacac acagatacta
    13021 taccgcgaaa acacttttgt ggcaagtgta aacttttttg gggaatcatt gcggttttgt
    13081 ggcacatttg ctataataaa cacaaattcc cgccaaagtg aggtgtcctg catgaaaaaa
    13141 catccgttgc ttactgcggc cgccgcagcc ggtactgtcg tgctcggcgc agcctacggc
    13201 atctaccgcg tcgcctttta ccaaaagccc accaccgccg tgccctcccc cgccgatatg
    13261 cccacgcggg aggtctaccg caaggccatg tgccccgacg ccgacgcgct ggcgcgggac
    13321 accttcacgc cggtacatat caccgcgcag gacggcacac cgctggccgc acgatactac
    13381 caccacaacg acggtgcccc gctggctatc atcttccacg gctaccgggg ctatgccgtg
    13441 cgggacggcc tgggcggcta caccctctgc accaagctgg gttataatgt tctgctgccc
    13501 gaccagcgcg cccacgggta cagcggcggc cacaccatta ccatgggcgt gaaggagcgc
    13561 tacgacgccc gggactgggc tgtctgggca cgcagccact ttggcccgga agtccccatc
    13621 tttttgatgg gcgtctcgat gggcgcagcc accgtgctgc tggccgccgg gctgaatctg
    13681 ccggacaacg tctgcggcat cgtagccgac tgcggctaca ccagcccgcg cgagatcacc
    13741 cgcaaatgcc tgccggagta cctacccggc ttccctctcg gccccaccta cgccatcggg
    13801 cggctggggg ccatcctgtt cggccatttt gaccccgagg atgctgactg caccgatgcc
    13861 gcggcccaca gcaaagtgcc gatcttcttc gtccacggcg atgccgacgg ctttgttccc
    13921 tacgagatgg gccgccgcaa ctacgaggtc tgccgcgcca agggcaagcg ctttttgacg
    13981 gtgcacggtg ccgagcacgc cgtcagctac taccacgaca acaccgccta cacgaaagaa
    14041 tgcaccgact tcctgcaaga ctgcctcacc cgccgccact tcgcctggcc gaaggaagcc
    14101 cccgacgacg cgcgataacc gcattgccac cccgtagggc ggtcagtccc ctcgccgctc
    14161 ctctcatttg tagggggcgg cgtcctcgac gccccgcata tcgtccgctc tgttaccccc
    14221 gtttttctaa aaaatcaaac atagag
//
LOCUS       DBMDSQ010000024        14025 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_1349_length_14025_cov_2.118357, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000024 DBMDSQ010000000
VERSION     DBMDSQ010000024.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 14025)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 14025)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14025
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1349_length_14025_cov_2.118357"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            460..1401
                     /locus_tag="ACZPAX_01775"
     CDS             460..1401
                     /locus_tag="ACZPAX_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007048396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HIR1112660.1"
                     /translation="MMKIYRTQDKQLTRVDDMSEGAWICLTSPTDEEVRRVAATLDIE
                     PTDIVAATDPEESARISLEDGYTVIIVDIPIKVDGASEGVYTTIPLGILLTQELIVTV
                     CSADTAVVGDFAACRVKGFSTRKKMRFVYQLLYRAATMYQQELRLIDRRRQAIEKNLA
                     GELKDSDLMELHALESTLVYFATSLRANATVLDRLTRYKRLEQYPDDRELLDDVIVEI
                     RQAIEMTSIYRDDIKGTRELFSSILDNRLNNAMKYLTSITLLMAVPTVISGLYGMNVQ
                     SDGMPFANTTAGFAIVLALTLALCVFAAWVLHKKHML"
     gene            1519..2079
                     /locus_tag="ACZPAX_01780"
     CDS             1519..2079
                     /locus_tag="ACZPAX_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016321342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR1112661.1"
                     /translation="MEDALIVDLYWARDEQALAETAAKFGTYCRKIADNILHSAHDAE
                     ECENDTWLAAWNSMPTNRPARLAPYLGRITRNLALDRFDKASAQKRGSGQTCAPLDEL
                     AECVAAPGGVEDSFDAAETGRIISDFLRTLPEETRNIFLRRYWYCDATADIAARYGLT
                     ESKVRVTLHRTRGKLAAYLQERGAAL"
     gene            2076..2675
                     /locus_tag="ACZPAX_01785"
     CDS             2076..2675
                     /locus_tag="ACZPAX_01785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1112662.1"
                     /translation="MTERELFEAIGHVDDDLILAADAPLKKRRKPPVYWRQLVSVAAC
                     ACIFLGGVAVWQSDGFRKANMTSEAAAFADDAPEEAAEIAPAPDAPMLSAAALQADNG
                     DAAAKEGAAKDSAESSIALGLARCVQIDGVRYFESPKVIDFIANRDPDGVISDSVGRD
                     AFPETDRCSNFGTGYSYWVTGDNAVVVEIDGEYCEFEAN"
     gene            complement(2885..4123)
                     /locus_tag="ACZPAX_01790"
     CDS             complement(2885..4123)
                     /locus_tag="ACZPAX_01790"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007048262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HIR1112663.1"
                     /translation="MPKIYFHVDVNSAFLSWSALKSLQDGGTVDLRTIPAVVGGDEAK
                     RHGVVLAKSAPAKKFGIQTGESLFAARAKCPGLTIVPPDFDFYVKNSKALIAILNDYT
                     PDVEQYSIDEAFLDMTGTEALFGPPLTAAHTIRRRVRRELGFTVNVGIAPNRLLAKMA
                     SDFEKPDKVHTLYRYEVEKKMWPLPVGDLFGVGPSAAKRLNGCGVYTIGDFAKLDRET
                     AVGLFGTRGDTLWNYANGRESDPVTKQGSRDNSYGNSVTMPRDLPRPEDADATMLALC
                     DSVGRRLRADGKTARVVTVQLVDNAFRRTSHQTTLPNPTNSTDRLYHVATELMRQMWP
                     ARPVRLVGVSAEKTSEDNFEQMDLFTDAARIDKQEKLDRAADALRKKFGGAAVTRAKL
                     LTPETRKPEALSAAKERDKK"
     gene            4286..5272
                     /gene="argF"
                     /locus_tag="ACZPAX_01795"
     CDS             4286..5272
                     /gene="argF"
                     /locus_tag="ACZPAX_01795"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004603950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - L-arginine biosynthetic process
                     via ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HIR1112664.1"
                     /translation="MDLVGHDFLTLLDFTPAEIGGLLDLAAELKAKKKAGIPHDTLRG
                     KNVALIFEKTSTRTRCSFEVAAHDLGMGTTYLDPTGSQIGKKESIADTAQVLSGMFEG
                     IEYRGYGQAIVEELAKYASVPVWNGLTNEYHPTQILADFLTLREHFGKLQGLKFAYMG
                     DARYNMGNSLMIGCAKMGLHFVACAPKAYWPNPELVAKCREFARLSGGSITLSEDTDA
                     VAGADVVYTDVWVSMGEPVEVWEERINALAPYQVNAQLMAKAKGTAVFMHCLPAYHDH
                     KTAVGKEMGEKFGRDAMEVTDEVFNGPQSIVFQEAENRMHTIKAVMAATLGA"
     gene            complement(5541..7262)
                     /locus_tag="ACZPAX_01800"
     CDS             complement(5541..7262)
                     /locus_tag="ACZPAX_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018211528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR1112665.1"
                     /translation="MKNKRIIAAAMAAGMAVSLAACGSSASTTDTNGSADTATTEAAD
                     AATGEGTGFTVQLGPNPETLDPALNAAVDGGNTLITIEEPLLIIDENNEIQPGQAESY
                     EVSDDGLVWTFHLRDGLKWSDGSDLTAADFEYSFKRLACPDTAAPYAETVVGMIDGYQ
                     DAIGNPDADGNTTTDPDWDALNVVASEDGKDLTITLSYPCSYFDKLAAFVATSPVQQA
                     TVEANGDAWCTEPDTYVCNGPYMITDWIPSERIVLSKNPNYVGGWDSSKIVSDTITLL
                     LLEDSSAAYAAYNSGEAQLVKDVPTDEIPSLTRAEDGGDFYLDEIMGTYYISLNDQKE
                     PFTDPNVRKAMSLAIDRDYVANTIMQGIYTPATALVGPGIVDNEGYFMDNANGGKPYI
                     GDDYEANLEEAKQLMAEAGYPDGEGFPVVEYSANDAGYHIPVAEYVQQAWGELGITVN
                     INKVEWASFLPMRRAGDYDISRNGWSMDYNDPSNMLELFTTNNGNNDGKYANPDFDKV
                     IEESRVADKATHFEKLHEAEDMLMADAACIPVAYYNDFWLQSPSLKGTWHSPYGYWYL
                     QYGYVEE"
     gene            complement(7473..9200)
                     /locus_tag="ACZPAX_01805"
     CDS             complement(7473..9200)
                     /locus_tag="ACZPAX_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014794298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR1112666.1"
                     /translation="MKMKNVAAVAMAGCMAASLAACGGSASTAASTETSTAASTEAAT
                     GEESTGTANGFTVEYGPNPETLDPALNSAIDGANTLITVFEPLLLIDQNNEVIPGQAE
                     SYTVSDDGLVWTFTMRDGLKWSDGSDLTAKDFEYSFKRLAAPDTAAPYAETVVGMIDG
                     YEDAVGNPDADGNMTTEPDFDALNVVASEDGKTLTVTLSYPCAYFDKLAAFAAMSPVQ
                     QATVEANGDAWCTQPDTYVCNGPYMITDWVPSERIVLSKNPNYVGGWDSSKIVSDTIT
                     LLLLEDSSAAYAAYNSGEAQLIKDVPTDEIPSLTKAEDGGDFYVDTILGTYYISLNDQ
                     REPFTDPNVRKALSLAIDRDYVANTIMQGTYTPAYNLVGPGIVDENGMFYDNANGGKT
                     YISDDYEANLEEAKQLLADAGYPNGEGFPTIEYSTNDAGYHTPVAEYVQQAWGELGIT
                     VNINKVEWASFTPLRRAGDYDVSRNGWVMDYNDPSNMIELFTTTNGNNDGKYSNPEFD
                     AAIEASKVADKSVHFQKLHEAEDILMADAACIPVAYYNDFWLQSPSLKGTWHSPYGYW
                     YLQYGYVEQ"
     gene            complement(9561..10550)
                     /locus_tag="ACZPAX_01810"
     CDS             complement(9561..10550)
                     /locus_tag="ACZPAX_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018305960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1112667.1"
                     /translation="MSENLVEVKHLQQYFPAGGVGKNKQYVQAVDDVSFAIRKGETLG
                     LVGESGCGKTTTGRTLLRLYEPTDGTIIYDGKVLFDKKKKIAVDMLPYRRRMQIVFQD
                     PYASLDPRMTIGDIVGEGIDIHHLCANAKERHDKIISLLERVGLNSEHANRYPHEFSG
                     GQRQRVGIARALAVDPEFIVCDEPVSALDVSIQAQVVNMFEDLQQEMGLTYLFIAHDL
                     SVVKHISNRIGVMYLGKLVELADSFELIAHSVHPYTRSLISAIPVADPVMARQSHRIV
                     LQGDVPSPLNPPSGCRFRTRCPYADERCAAEVPEFKEVSSGHWAACHHLDKVK"
     gene            complement(10547..11548)
                     /locus_tag="ACZPAX_01815"
     CDS             complement(10547..11548)
                     /locus_tag="ACZPAX_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015537840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1112668.1"
                     /translation="MSEKILEIKDERLSFFTPAGEVKALNGVSFSMNQGDVLGIVGES
                     GSGKSVTAYSIMGLTAYPGRLVGGTVWFNGHQIDKMTEKEFRKIRGNEVSIIFQDPMT
                     SLNPVYTIGNQIVEVILLHTKKTKQEAWARAKELLELVGINEPDRRLKQYPHELSGGM
                     RQRVMIAIALACEPKLLIADEPTTALDVTIQAQILELMNDLRHKLGMSIIMITHDLGV
                     VAQMCEKIAVMYAGHIVEYGTTDEIFYNPQHEYTKGLINSIPKLNAEEKERLVPIEGQ
                     PVDLLNPPAGCPFAPRCKSCMKVCLNKMPPKTELSDTHYTYCWLRQKEEFEKGGKAE"
     gene            complement(11568..12617)
                     /locus_tag="ACZPAX_01820"
     CDS             complement(11568..12617)
                     /locus_tag="ACZPAX_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005923262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1112669.1"
                     /translation="MENLKKNPLSLQLNVEDFAPASNDEKKSLVVMRESVNFWKDGMR
                     RLKKNKIAMVSLVVILLIAFMAYILPSLWPYSYEQQIKGSNNLAPFEYSAAEQKLIDQ
                     GENVFPHILGTDRMGRDFAVRVMMGTRVSLSVGLLASVLVLLIGATYGAISAFAGGWV
                     DNIMMRITDILYTIPDILLIILLAMAIKEPLEALATKPGFGWMQTLGPNMVSIFIIFA
                     LLYWVGMARIVRSQVLTLKESEYVTAARALGASGGRIIKKHLLTNCMGTLIVTTTLQI
                     PSSIFTESYLSFLGLGVAAPMPSLGSLATDAVKGMNTYPVLLIAPALMISVMILVFNL
                     FGDGLRDAFDPKLKS"
     gene            complement(12633..13550)
                     /locus_tag="ACZPAX_01825"
     CDS             complement(12633..13550)
                     /locus_tag="ACZPAX_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014794302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1112670.1"
                     /translation="MAKYIVKRVAMGIFSVFIVATLTFFIMNLVPGGPFVAEKSISKA
                     AQQALAEKYGLDKPLGVQYLNYMNSLVHGDMGLSLKQRGRTVNQIIFSKLPVSARTAG
                     LAVLVALCVGIPLGCLSAYNRGKWADNLIIVFATCGIAIPSFISSVVLLYTFGMNLKI
                     LPTVGLNEPAAYIMPVTALAIYPTAYITRLMRSSLLDVMGQDYMRTARAKGVSSVKIL
                     FKHALRNAILPVVTYVGPMLAGLMTGSFVVEKIFSIPGLGRDFVSAITNRDYMMIMGT
                     TILLATLVIVANVIVDILYKIIDPRIKLK"
     gene            13938..14014
                     /locus_tag="ACZPAX_01830"
     tRNA            13938..14014
                     /locus_tag="ACZPAX_01830"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13972..13974,aa:Arg,seq:acg)
BASE COUNT         2777 a         3884 c         4452 g         2912 t
ORIGIN      
        1 tatgaattac ttgtaaattt ggaattgatt atcgtgggtt tccaaaccca cgttcaaggt
       61 tccacaagtt tcagttttgt tcaattttca aggtcctgct gtgctgtcgc gtctccgtga
      121 cagcttgtat attatagcct gtccaatcaa aaatgtcaat agggttttta aaagtttttt
      181 ggattttttt caggaggggc atttgagggg aaattgggtg gtataggcaa ggcggagggc
      241 cgggcatgcc cggcccctac ggcgcaacag atgggggaaa gttggtgatg cgggcagggt
      301 gcgcgggcgg catatatgcc gcccctacgg gtgggccgtt gggtgagtgg ttgccgtaaa
      361 gggtttggtt gcggggtgca tcgggggaaa atatctgctt gcgcatcgtg cggaaaagct
      421 ttatactgat actataataa tgtatgggtg gaacgagcca tgatgaagat ttaccgcacg
      481 caggacaagc agctgacgcg ggtcgatgat atgagcgagg gggcgtggat ctgcctgacc
      541 agccccaccg atgaggaagt gcgccgggta gcggcaacac tggacatcga gcctaccgat
      601 atcgtcgcgg caaccgaccc cgaggaatcg gcgcgtatca gcctggagga cggctacacc
      661 gtcatcatcg tggacatccc catcaaggtg gatggcgcga gcgagggcgt gtacaccacc
      721 atccccctgg gcatcctgct gacgcaggag ctgatcgtca cggtctgctc cgctgatacc
      781 gccgtggtcg gcgatttcgc cgcctgccgt gtcaaggggt tctcaacgcg gaagaagatg
      841 cggttcgtct accagctttt gtaccgcgcc gccactatgt atcagcagga actgcggctc
      901 atcgaccgcc gccgccaggc cattgagaag aatcttgccg gagagctgaa ggacagcgac
      961 ctgatggagc tgcacgcgct ggaatccacg ctggtctact ttgccaccag tctgcgcgcc
     1021 aacgccacgg tgctggaccg cctgacccgc tacaagcggc tggagcagta ccccgacgac
     1081 cgcgaattgc tggacgacgt tatcgtcgaa attcgccagg ccattgagat gaccagcatc
     1141 taccgcgacg atatcaaggg cacacgagaa ctgttcagct cgattttgga caaccgcctg
     1201 aacaacgcca tgaaatacct gaccagcatc accctgttga tggccgtgcc caccgttatc
     1261 agcggtttgt acggcatgaa cgtccagtcc gacggtatgc cgtttgccaa caccaccgcc
     1321 gggtttgcca ttgtgctggc gctgacgctg gctttgtgcg tgtttgcggc gtgggtgctg
     1381 cacaaaaaac atatgctgta aggcgggcac acacaagtgt acccccacaa aaatgtaacg
     1441 cctgccgcaa aaagcaggcg ttttgtgtaa catttgcagg gcaaatccct accttaatat
     1501 ataaaggagg tcaaaaccat ggaggacgct ttgatcgtgg acctgtactg ggcccgggac
     1561 gagcaggcac tggccgagac ggcggccaag ttcggcacgt actgccgcaa aattgccgac
     1621 aacattctgc acagcgccca tgacgccgaa gaatgcgaga acgacacctg gctggccgcg
     1681 tggaacagta tgcccaccaa ccgccccgcg cggctggcgc cgtacctggg gcgcatcacg
     1741 cggaatctgg cgctggatcg ctttgacaag gcatcggccc agaagcgtgg cagcggacag
     1801 acctgtgccc cgctggacga gctggccgag tgcgtcgccg cgccgggcgg cgtggaggac
     1861 agttttgacg ccgccgaaac cggacggatc atcagcgatt ttctgcgcac tttgccggaa
     1921 gaaacacgca atatattttt gcgccgctat tggtattgcg acgccacggc agatattgcc
     1981 gcgcggtacg gtctgactga gagcaaggtg cgggtgacgc tgcaccgcac gcgcggcaag
     2041 ctggccgcat acttacagga aaggggtgct gcgctgtgac agaacgtgaa ttgtttgagg
     2101 ccatcggcca tgtggacgat gacctgattt tggccgccga cgcgccgttg aaaaagcgcc
     2161 gcaaaccgcc ggtatactgg cggcaactgg tgtcggtggc ggcgtgcgcg tgcatcttcc
     2221 tgggcggcgt ggccgtgtgg cagagtgacg gattccgcaa agccaacatg accagcgaag
     2281 ccgctgcctt tgccgacgac gccccggagg aagccgccga gattgccccc gcaccggatg
     2341 caccaatgct gagcgccgct gcgctgcagg cagacaatgg ggacgcggcc gccaaggagg
     2401 gggcagcaaa ggattctgcc gagtcgtcta tcgcactggg cctggcacgg tgcgtgcaga
     2461 tcgacggcgt gcgttatttt gagtcgccga aggtgattga tttcatcgct aaccgcgacc
     2521 cggacggcgt gatctccgac agcgtcggca gagacgcatt tcctgagaca gacaggtgct
     2581 cgaattttgg cactgggtat tcgtactggg tgaccgggga caacgccgtg gtcgtggaga
     2641 tcgacgggga atattgcgag tttgaggcga attgagggtt ctacgcgcct ccccctttgg
     2701 gggaaggtgg ccccgcaggg ccggatgagg ggcgcgtgta ccgcggcacc ccgatagcgg
     2761 acggcacggc aaggctgccc ctcatccgtc agcggtcggg gccgctgaca gcttccccct
     2821 cgggggaagc catgctcaca caaagtacaa atgacccatg agaggcagtc accgtctcca
     2881 tgggtcattt tttatcccgc tctttggcgg cgctcagggc ttcgggtttg cgggtctcgg
     2941 gggtcagcag tttggcgcgg gtcacggcgg caccgccgaa ctttttccgc agggcgtctg
     3001 cggcgcggtc gagcttttcc tgcttgtcga tgcgggcggc atccgtgaac aggtccatct
     3061 gctcaaagtt gtcctcgctg gttttttcgg cgctgacacc caccagccgc accgggcgcg
     3121 cgggccacat ctggcgcatc aactcggtgg cgacgtgata gagccggtcg gtggagttcg
     3181 tcggattggg cagtgtcgtc tggtggctgg tgcggcgaaa ggcgttatcc accagctgca
     3241 ccgtcaccac gcgggcggtc ttgccgtcgg cccgcaggcg gcggcccacc gaatcacaga
     3301 gcgccagcat cgtggcgtcg gcgtcctcgg gccggggcaa gtcccggggc atcgttacgc
     3361 tgttgccgta gctgttgtca cggctgccct gcttcgtcac cgggtcagac tcgcggccgt
     3421 tggcgtagtt ccacagcgta tcgccccggg tgccgaacag ccccaccgcc gtctcgcggt
     3481 cgagcttggc gaaatcgccg atggtgtaga ccccgcaacc gttcagccgc ttggccgcac
     3541 tggggccgac accgaacaga tcgcccaccg gcagcggcca catcttcttt tcgacctcgt
     3601 atctatataa ggtatggacc ttgtcgggct tttcaaagtc ggaggccatt ttggcaagca
     3661 ggcggttggg cgcgatgccg acattgaccg tgaagcccag ctcccggcgc acacggcggc
     3721 ggatggtgtg ggcggcggtc agcggcgggc cgaacagcgc ctcggtgccg gtcatgtcaa
     3781 ggaaggcctc gtcgatgctg tattgttcaa catccggggt gtagtcgttc aaaatagcaa
     3841 tcagtgcctt gctgtttttg acgtagaagt caaagtccgg cggcacgatg gtcagccccg
     3901 ggcacttggc gcgggcggcg aaaaggctct cccctgtctg gatgccgaat tttttcgcag
     3961 gggcgctttt ggccagcaca acgccgtggc gcttggcctc gtcgccgccc acaacggcgg
     4021 ggatggtgcg caggtcgacg gttccgccgt cctgcagcga tttaagcgcc gaccatgaga
     4081 ggaaggccga gttgacatcg acatggaagt agattttggg catgggtgag gcggcctcct
     4141 ttccattctc tttgccttct ccccgagggg gaagccttta taaagattat accacaaact
     4201 cgctcttgca catagcctgc aaacgtggta taatcatatg tatatttata cagataatac
     4261 gtatatttac aaaaggagca accatatgga cctcgtagga catgattttt tgacgctgtt
     4321 ggactttacc ccggctgaga ttggcggcct gctggacctg gcggccgagc tgaaagccaa
     4381 gaaaaaggcg ggcattcccc acgatacgct gcgcggcaaa aacgtggcgc tgatttttga
     4441 aaagacctcc acccgcaccc gctgctcgtt cgaggtcgcg gcccacgact tagggatggg
     4501 caccacctac ctggacccca ccggcagcca gatcggcaaa aaggagtcca tcgccgacac
     4561 ggcgcaggtg ctctccggta tgtttgaggg catcgagtac cgcggctacg gccaggccat
     4621 tgtggaagag cttgccaagt acgccagcgt gccggtgtgg aacggcctga cgaacgagta
     4681 ccaccccacc cagatcctgg ccgacttttt gacgctgcgt gaacatttcg gcaagctgca
     4741 gggcctgaaa tttgcctaca tgggcgacgc ccgctacaac atgggcaaca gcctgatgat
     4801 cggctgcgcg aagatgggcc tgcattttgt ggcctgcgca cccaaagcct actggccgaa
     4861 ccccgagctg gtcgccaaat gccgggagtt tgcccgcctg agcggcggca gcatcacact
     4921 ttccgaggat accgacgctg tggccggggc cgatgtggtc tacactgatg tgtgggtcag
     4981 catgggtgag ccggtggaag tgtgggagga gcgtatcaat gccctggccc cctaccaagt
     5041 caacgcgcag ctgatggcga aggccaaggg caccgccgtg ttcatgcact gcctgcccgc
     5101 ctaccacgac cacaagaccg ccgtgggcaa ggaaatgggc gagaaatttg gccgcgacgc
     5161 catggaagtg accgacgagg tattcaacgg cccgcagtcc atcgtattcc aggaggccga
     5221 gaacagaatg cataccatca aagccgtgat ggcggcaacg ctgggggcgt gagtcccaag
     5281 ccttcccctg tgggggaagg tggccccgca gggccggatg aggggcaaag tttgccgtgg
     5341 caacccgatt gcgggcgaca atggtaaggt ttttcctcat cagtcagcgg gcggggccgc
     5401 tgacagcttc agtctccgcg ctaagagccg cctacggcgg ttgcgctccg acacgcgcct
     5461 gcgggcgcag ccccaagggg gaagccagac acagaaaaag gcacgtccaa cgcaggacgt
     5521 gcctttccat ttataaagaa ttactcctca acataaccat actgcaggta ccagtagccg
     5581 taggggctgt gccaggtgcc cttcagagag gggctctgca gccagaagtc gttgtagtag
     5641 gcaacgggga tgcaggcggc gtcggccatc agcatgtcct cggcctcgtg cagcttctca
     5701 aagtgggtag ccttgtcggc aacgcgggac tcctcgatga ccttgtcgaa gtcggggttg
     5761 gcgtacttgc cgtcgttgtt gccgttgttg gtggtgaaca gctccagcat gttggagggg
     5821 tcgttgtagt ccatggacca gccgttgcgg ctgatgtcat agtcaccggc gcggcgcatc
     5881 ggcaggaagc tggcccactc gaccttgttg atgttgacgg tgatgcccag ctcgccccag
     5941 gcctgctgga cgtactcagc aacggggatg tggtagccgg cgtcgttggc gctgtactca
     6001 acaaccggga agccctcgcc gtccgggtag ccggcctcgg ccatcagctg cttagcctcc
     6061 tcgaggttgg cctcgtagtc gtcgccgatg tagggcttgc cgccgttggc attgtccatg
     6121 aagtagccct cgttgtccac gatgccgggg ccaaccagcg cagtggcggg ggtgtagatg
     6181 ccctgcatga tggtgttggc aacgtagtcg cggtcaatgg ccagagacat agccttgcgg
     6241 acattggggt cggtgaacgg ctccttctga tcgttcaggg agatgtagta ggtgcccatg
     6301 atctcatcga gatagaagtc gccgccgtcc tcggccctgg tcaggctggg gatctcatcg
     6361 gtggggacgt ccttgacgag ctgtgcctca ccgctgttgt aggcggcgta ggcagcgctg
     6421 gaatcctcga gcagaagcag ggtgatggtg tcggaaacga tcttggagga atcccagccg
     6481 ccgacgtagt tcgggttctt ggacagaaca atacgctcgg aggggatcca atcggtgatc
     6541 atgtaggggc cgttgcagac gtaggtgtcg ggctcggtgc accaggcgtc gccgttggcc
     6601 tccacagtgg cctgctgcac gggtgaagtg gcgacgaaag cagccagctt gtcaaagtag
     6661 gagcagggat agctcagggt gatggtcagg tccttaccgt cctcgctggc aacgacgttc
     6721 agggcgtccc agtcgggatc ggtggtggtg ttgccgtccg catcggggtt gccgatggca
     6781 tcctgatagc cgtcgatcat gccgacaacc gtctcggcgt agggggcggc ggtgtcgggg
     6841 caggccagac gcttgaagct gtactcgaag tcggcagcgg tcaaatcgga accgtcgctc
     6901 cacttcaggc cgtcgcgcag gtggaaggtc cagaccaagc cgtcatcgct gacttcgtag
     6961 ctctcggcct gaccgggctg gatttcgttg ttctcgtcga tgatgagcag gggctcctcg
     7021 atggtgatga gggtgttgcc gccgtcgacg gcagcgttca gggccgggtc cagggtctcg
     7081 gggttggggc cgagctggac ggtgaagccg gtgccctcgc cggtggcggc gtcagcagcc
     7141 tcggtggtgg cagtatcggc gctgccgttg gtgtcagtgg tgcttgcgga agaaccgcag
     7201 gctgccagag aaactgccat gccggcagcc atcgccgcgg cgatgattct cttgttcttc
     7261 atgttgcttg tatcctcctg atggatcttt cttacactcg ttgtctgtcc gtgacaggcg
     7321 aacattgact attatagtac gcgcaacaca ggattgcaag ttcggcaacg atatgttact
     7381 gttttgtaac ggttttgcct tccccaaggg gaagcctcac caacaaaaaa aggcacggcc
     7441 ttttaacagg tcgtgccttt tataaaggtt agttactgct caacataacc gtactgcagg
     7501 taccagtagc cgtaggggct gtgccaggtg cccttcagag aggggctctg cagccagaag
     7561 tcgttgtaat acgcaacggg gatgcaggcg gcgtcggcca tcaggatgtc ctcggcctcg
     7621 tgcagcttct ggaagtggac gctcttatcg gcgaccttgg aggcttcgat ggcggcatcg
     7681 aactcggggt tgctgtactt accatcgttg ttgccgttgg tggtggtgaa cagctcgatc
     7741 atgttggaag gatcgttgta gtccatgacc cagccgttac ggctgacgtc gtagtcgccg
     7801 gcgcggcgca acggggtgaa gctggcccac tcgaccttgt tgatgttgac ggtgatgccc
     7861 agctcgcccc aggcctgctg gacgtactca gcaacggggg tgtggtaacc ggcgtcgttg
     7921 gtgctgtact cgatggtggg gaagccctcg ccgttcgggt aaccggcatc ggccagcagc
     7981 tgcttggcct cctcgaggtt ggcctcgtag tcgtcgctga tgtaggtctt gccgccgttg
     8041 gcgttgtcgt agaacatgcc gttctcatcg acgatgccgg ggccgaccag gttgtaagcg
     8101 ggggtgtagg tgccctgcat gatggtgttg gcaacgtagt cacggtcaat ggccagagac
     8161 agagccttgc ggacattggg gtcggtgaac ggctcgcgct ggtcgttcag ggagatgtag
     8221 taggtgccca ggatggtgtc aacatagaag tcgccgccgt cctcagcctt ggtcaggctg
     8281 gggatctcat cggtgggaac atccttgatg agctgtgcct caccgctgtt gtaagcggcg
     8341 taagcagcgc tggaatcctc cagcaggagc agggtgatgg tgtcggaaac gatcttggag
     8401 gaatcccagc cgccgacata gttcgggttc ttggacagaa caatacgctc ggaggggacc
     8461 caatcggtga tcatgtaggg gccgttgcag acgtaggtgt cgggctgcgt gcaccatgca
     8521 tcgccgttgg cctcaacagt ggcctgctgc acagggctca tggcggcaaa ggcagccagc
     8581 ttgtcaaagt aagcgcaggg gtagctcagg gtgacggtca gggtcttgcc atcctcgctg
     8641 gcaacgacat tcagggcgtc gaagtcaggc tcggtggtca tgttgccgtc tgcatcgggg
     8701 ttgccgacgg catcctcata gccgtcgatc atgccgacaa cggtctcagc atagggggca
     8761 gcggtgtcgg gcgcggccag acgcttgaag gagtactcaa agtccttcgc ggtcaggtcg
     8821 gagccatcgc tccacttcag gccgtcacgc atcgtgaagg tccagaccag gccgtcatcg
     8881 ctgacagtgt agctctcagc ctggccggga atgacctcgt tgttctgatc aatgagcagc
     8941 agcggctcaa agacagtgat cagcgtgttg gcgccgtcga ttgcgctgtt cagcgcgggg
     9001 tccagagtct cggggttagg tccgtactcg acagtgaaac cgttggcagt gccagtgctc
     9061 tcttcgccgg tggcagcttc ggtggatgca gcggtgctgg tctcggtgga ggctgcggtg
     9121 ctggcagagc cgccgcaggc agcgagagaa gcagccatgc agccggccat agccacggca
     9181 gcaacgttct tcatcttcat gtgaaatacc ctccaaattc attatacttt atacccaatc
     9241 cccctcgggg gcagcgcccc caacggagtg aatatttata ctttaccact ctaaagcggc
     9301 atttgcaagt gttctgtaat ttttgtaact gttttgatag aaaatccgtt tgtatctccg
     9361 ccgcggacaa ttctgtgtgt cgcagataga tgtctctcta ctgcatacag ttttgcttaa
     9421 aacgcaaggc ggccacggct gaccgccttg catatgtata aatttcaggc cgagtgaagt
     9481 cagtcggcgg acagaatcgt ccaaaggccc tcacggccgc cgtccagcgg gttgcagggg
     9541 cgtcgaggac gcccccctac ttattttacc ttatccagat ggtggcaggc tgcccaatgg
     9601 ccgctgctga cctccttgaa ctccggcacc tcggcagcgc agcgctcatc ggcgtagggg
     9661 cagcgggtgc ggaaacggca gccgctcggc gggttcagcg ggctgggcac atcgccctgc
     9721 agcacaatgc ggtggctctg gcgggccatc acggggtcag ccaccggaat ggccgagatc
     9781 aggctgcggg tgtaggggtg gacgctgtgg gcgatcagct caaagctgtc ggccagctcg
     9841 accagcttgc ccagatacat aacgccgatg cggttggaaa tgtgcttgac aacgctcagg
     9901 tcgtgcgcaa taaagaggta ggtcagcccc atctcctgct gcagatcctc aaacatattg
     9961 acgacctgtg cctgaattga gacatccagc gcggagacag gctcgtcgca gacgatgaac
    10021 tcagggtcca cagccagcgc acgggcgatg ccgacacgct gacgctgacc gccggagaac
    10081 tcgtgcgggt agcggttggc gtgctcactg ttcaggccga cacgctccag cagggagatg
    10141 atcttgtcgt ggcgctcctt ggcgttggcg cacaggtggt ggatgtcgat gccctcaccg
    10201 acgatatcac cgatggtcat gcgggggtcc aggctcgcgt aggggtcctg gaacacgatc
    10261 tgcatacggc ggcggtaggg cagcatatcc accgcgatct tctttttctt gtcaaacagc
    10321 accttgccgt catagatgat ggtgccgtcc gtcggttcat acaaacgcag cagggtgcgg
    10381 ccagtggtgg tcttgccgca gccggattcg ccgaccagac ccagggtctc gcccttgcgg
    10441 atggcaaagc tgacgtcatc gacagcctgc acatactgct tgttcttgcc cacgccgccg
    10501 gccgggaagt actgctgcaa atgctttact tcaacaagat tctcgctcat tcggctttgc
    10561 cccctttctc aaattcttct ttctggcgca gccagcaata ggtgtaatgg gtgtcgctca
    10621 attcggtttt gggcggcatt ttattcaggc agaccttcat gcagctcttg cagcgcggcg
    10681 cgaaggggca gcccgccggc gggttgagca ggtcaacggg ctggccctcg atgggcacca
    10741 aacgttcctt ctcctcggcg ttcagcttgg ggatggagtt aatcaggccc ttggtgtact
    10801 cgtgctgggg gttgtagaaa atctcgtcgg tggtgccgta ctcgacgatg tgaccggcgt
    10861 acatgacggc gatcttctcg cacatctggg cgacaacacc caggtcatgg gtgatcatga
    10921 tgatggacat acccagcttg tggcgcaggt cgttcatcag ctcaaggatc tgcgcctgga
    10981 tcgtgacgtc cagcgcggtg gtcggctcgt cagcgatcag cagcttcggc tcgcaggcca
    11041 gcgcaatggc gatcataaca cgctggcgca taccgccgga cagctcgtgg gggtactgct
    11101 tcaggcggcg gtccggctcg ttgatgccga ccagctccag cagctcctta gcgcgggccc
    11161 aggcttcctg cttggtcttt ttggtgtgca acaggatgac ctcgacgatc tgattgccga
    11221 tggtgtacac cgggttcaga ctcgtcatag ggtcctggaa gatgatggag acctcgttgc
    11281 cgcggatctt gcggaactcc ttctcggtca ttttgtcgat ctggtggccg ttgaaccaca
    11341 ccgtgccgcc gaccagacgg ccggggtaag cggtcaggcc catgatggaa tacgccgtga
    11401 cagacttgcc ggagccggac tcgccgacga tgcccagcac gtcgccctgg ttcatgctga
    11461 aggacacgcc gttcagcgct ttgacttcgc ccgcgggggt gaagaaggac aggcgttcgt
    11521 ccttgatttc aagaattttc tcgctcatgt ttgcttctcc tcccctctca gctcttcagt
    11581 ttcgggtcaa atgcgtcgcg caggccatcg ccgaacaagt tgaaaaccag aatcataacg
    11641 ctgatcatca gcgcgggggc aatcagcagc accgggtagg tgttcatgcc cttgacggcg
    11701 tcggttgcca gagagcccaa ggacggcata ggcgcagcaa cgcccaggcc gaggaagctc
    11761 aggtagctct cggtgaagat ggaggacggg atctgcaggg tggtggtgac gatcagggta
    11821 cccatgcagt tggtcagcag gtgctttttg atgatacggc cgccggatgc gcccagtgcg
    11881 cgggcagcgg tgacatactc gctctctttc agcgtcagga cctgggagcg gacgatacgc
    11941 gccatgccga cccaatacag cagcgcgaag atgatgaaga tcgacaccat attggggccg
    12001 agggtctgca tccagccaaa gccgggcttg gtcgccagcg cttccagcgg ttccttgatg
    12061 gccatggcca gcaggataat gagcaggatg tcggggatgg tgtagagaat atcggtaatg
    12121 cgcatcatga tgttatcgac ccagccgccc gcaaatgcgg agatagcgcc gtaggtcgca
    12181 ccgatgagca ggaccagcac agaggccagc aggccgacgg acagggagac gcgggtgccc
    12241 atcatgacac gcacggcaaa gtcacggccc atacggtcgg tgcccagaat gtgggggaat
    12301 acgttctcgc cctggtcgat gagcttctgc tccgcggcag aatactcaaa cggagccagg
    12361 ttgttgctgc ccttgatctg ctgctcgtag ctgtacggcc agaggctggg cagaatgtag
    12421 gccatgaagg caatcagcag gatgaccacc aggctgacca tggcgatctt gtttttcttc
    12481 aggcggcgca tgccgtcctt ccagaagttg acgctctcac gcatgacaac gaggctcttc
    12541 ttctcgtcgt tggatgcggg ggcgaaatct tccacgttga gttgcaggct caacggattc
    12601 tttttcagat tttccatttt gtctgctcct ccttacttca gcttgatgcg cgggtcgatg
    12661 atcttgtaca ggatgtcaac gatgacgttg gcaacaatga cgagggtcgc cagcagaatc
    12721 gtggtgccca tgatcatcat gtagtcacgg ttggtgatgg cagacacgaa gtcacggccc
    12781 aggccgggga tggagaagat cttctcgacg acgaaggaac cggtcatcag acctgccagc
    12841 atggggccga cataggtgac aacaggcagg atggcgttgc gcagggcgtg cttgaacagg
    12901 atcttcacgc tggaaacgcc cttggcgcgg gcggtgcgca tgtaatcctg acccatgacg
    12961 tccagcagac tggaacgcat caggcgggtg atgtaagcgg tggggtaaat agccagcgcc
    13021 gtcacgggca tgatgtaggc cgccggctca ttcaaaccga cggtgggcaa aattttcaga
    13081 ttcatgccga aggtgtaaag cagcacgacg ctggaaataa agctcgggat agcgatgccg
    13141 caggtggcga agacaataat aaggttgtca gcccatttcc ctcggttata cgcagacaga
    13201 cagcccagcg gaatgccgac gcacagggcg accagaacgg ccagaccggc ggtgcgggca
    13261 gacacaggca gcttggagaa gatgatctgg ttgacggtac ggccgcgctg tttcaggctc
    13321 aggcccatat cgccgtggac aaggctgttc atgtagttga ggtactgcac acccaggggc
    13381 ttgtccaggc cgtatttttc ggccagagcc tgctgggctg ctttactgat ggacttttcc
    13441 gccacgaacg ggccgccggg gaccaggttc atgatgaaaa aggtaagggt cgcgacgatg
    13501 aacacgctga aaatgcccat tgccacgcgc ttgacgatgt acttcgccat ggtttagtgc
    13561 cactccgttt cttaatggaa tacaaattgg ggtcgattct ggtttatctg tttaatgttc
    13621 tgtactgtgc acgcgcattt gtgcgtacac tgtggacacg aaagagaata aaaaagccga
    13681 tggcggctgc gattgcaaac accatcgttc gtaatccaaa atctatgaat attataaaat
    13741 gttgcaaagt atttgtcaat tctctgtcgc gctcctggca acgacaaaca gccgaaaagc
    13801 cgcgcgttta gctgcgaatt ttcggtaaag atgcccattt gcagattttt ttgagttttt
    13861 ttgaaaaacc gcttgacttt ccccaccgcc ggcgctataa taaaacacgt tctccggcga
    13921 gaacgccaag agaatatgcg ccggtagctc agctggatag agtgtctgac tacgaatcag
    13981 aaggtcgggg gttcgagtcc cttccggcgc accaagttaa aagct
//
LOCUS       DBMDSQ010000025        13782 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_1374_length_13782_cov_2.150793, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000025 DBMDSQ010000000
VERSION     DBMDSQ010000025.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 13782)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 13782)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13782
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1374_length_13782_cov_2.150793"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(127..3684)
                     /gene="smc"
                     /locus_tag="ACZPAX_01835"
     CDS             complement(127..3684)
                     /gene="smc"
                     /locus_tag="ACZPAX_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015536734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HIR1112671.1"
                     /translation="MRLKELEIQGFKSFPDKTKISIGQGITGVVGPNGSGKSNISDSI
                     RWVLGETSSKQLRGSGKMEDVIFGGTQTRGAMGYASVALTIDNSDHGLDMDADEVTIG
                     RRYYRSGESEYSINGQSVRLKDVYELLLDTGIGRDGYAIVGQGRIAEIVGAKSAERRE
                     IFEEASGIAKYRYRKNEAERRLDAAEGNLERLRDILGELEKRVGPLKRDSEKAQQFLE
                     LSEKRKSLEITLWVDAIRRANDTLRDQQRKYEAAEADYDRLSRQLDAFDEKNADLREE
                     TQQLMLKVEQANADIRAITEANAGSESEIAVLKNESEHSRFRIDEANTELERAGQGRE
                     SIDREAADHRAAIDALKDGVAALDTRVAELREALHALEEKAAASGERRDVIDAAMARL
                     QDTATAAKVRAASAQSAGEAAANRLAEARQQAETLQTDAAATDEERRRAERRFKDAQE
                     AVTRNDNIKAGLKLKLDSRRRQQAEAADNLQKADREKSAAAQRIHILEDLERNMDGYQ
                     QSVKAVMRAAGGGRLRGIIGPVAGIITVEKGYETAIETALGFALQNIVVEDQGCARAA
                     IGFLKDERAGRATFLPLDTVQGSRFTGRLTGTAEVAADLVKTNPKYQHIIDNLLGRII
                     VVEDLTEASAVARNLGYRNRIVTLDGQVINAGGSFTGGSTARSVGVFSRKQELDELRT
                     RLKKLEEKRTEAEKELAARKAEVDNLSAQLAGAEGEGMNAATEHVRAGLELERLTKAA
                     AQYEETARNIEAEIANQQAAVTQNETARAEAEKAQADAEAEMVRYTAELAALGESTGS
                     LTAEREKITGELSEKQMQRLADEKDISLHEAALEGLQSRTGEAEARARELHAAIDAAK
                     AKIEANTLKIAEMERTRGENQQRIAAAEETIRSANAARMEKEAAIAKLGQDNRALTDE
                     RERMSGEMARLAERRTAAETELNDTNSKLWEEYQLTESEARSQCVPFESLTELRRSVT
                     EVRGKIRGLGNVNVGAIEEYKEVKERYDFLKAQVTDVEKAKSELTKMIAELCSEMEEL
                     FTTSFKQINTHFQQIFRELFGGGHARLYLSDEANVLESGIEIEVSPPGKVIKNLSALS
                     GGEQALVAISIYFAILNVNPAPFCFLDEIEAALDDVNVARYAQYLRRMTDHTQFIVIT
                     HRRGTMEAADVLYGVTMQEDGVSKILRLDLDNVSADLIT"
     gene            complement(3768..4433)
                     /gene="rnc"
                     /locus_tag="ACZPAX_01840"
     CDS             complement(3768..4433)
                     /gene="rnc"
                     /locus_tag="ACZPAX_01840"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HIR1112672.1"
                     /translation="MAHKPEELQEVLHYTFKNPALLRIALTHTSFANESKVATTHNER
                     LEFLGDSVLSVVVADYLFHQSGRPEGELTRMRASLVSEDALFQFAQEIHLGEYLRLGH
                     GEDLGGGRNRPSVVSDAFEAVIAALYLDGGMETARNFILPFITEGKTAEDDYKTRLQE
                     VVQQDPSAVLKYEVTGETGPDHDKQFTVCVWRNGRLLAEGKGRSKKAAEQHAAKVALE
                     KLQ"
     gene            complement(4575..5603)
                     /gene="plsX"
                     /locus_tag="ACZPAX_01845"
     CDS             complement(4575..5603)
                     /gene="plsX"
                     /locus_tag="ACZPAX_01845"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009322479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HIR1112673.1"
                     /translation="MKILVDVMGGDNAPLCVLQGASQAAAEFGDGMTLTLLGEEKAIR
                     DCAAENKIDLAPFEIINCTENIDMHDDPVKAVRHKKDSSLVKGLTMLKNGEGDAFVSA
                     GSTGALHVGTSLIVRTVKGVKRPALATPMPGAKQNFLLLDCGANVECRPEMLNAFGTM
                     GSVYAEKVMGRTAPKVALVNNGAEDTKGTPTYREAHQLLKANPCIHFAGNIEPRYIMD
                     GEVDVVVCDGFVGNVVLKLTEGVAKTLLGMLKKIFLQNLITKLSYLGIKGGLGELKRM
                     MDSEEVGGAPLLGAAKPVIKAHGSSHAKGIKNAIRQAKLCVANDLCGTMEKALGEVAA
                     KKEEEADA"
     gene            complement(5600..6910)
                     /gene="trmFO"
                     /locus_tag="ACZPAX_01850"
     CDS             complement(5600..6910)
                     /gene="trmFO"
                     /locus_tag="ACZPAX_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002847051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HIR1112674.1"
                     /translation="MQLNIIGAGLAGCEAALWLADRGVQVDLYEQKPTKFSPAHKSEG
                     FAELICSNSLKAERPDSASGLLKIEMKMMGSHLLDAAETARVAAGGALAVDRDVFSTA
                     VTEMVEKHPNITVHREEVTALDESAPVLVASGPLTEGALAQAVAALTGDHRLSFYDAV
                     APIVTAESLDYDKVFAASRYGRGEADYLNCPFNKEEYENFHAALIAAERAPLHDFDGD
                     LTVYEGCMPIEVMAARGADTIRFGPLRPVGLRDPRTGHRPWAAVQLRAENTARTLYNL
                     VGFQTNLKWGEQKRVFSMIPGLEHAEFVRYGVMHRNTFLESPKVLTKQQFLADHPNVF
                     FAGQITGFEGYMESAASGLLAAHQILARLQGCELPPPPAATMCGALLDYITTPNKDFQ
                     PMGANMGILPRTEEINAIRDKRERYMALSQNAQDAMRAWTEEYK"
     gene            complement(6957..9041)
                     /gene="topA"
                     /locus_tag="ACZPAX_01855"
     CDS             complement(6957..9041)
                     /gene="topA"
                     /locus_tag="ACZPAX_01855"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HIR1112675.1"
                     /translation="MPKLVIVESPAKAKTIGKYLGRGYKVTASMGHVRDLPASTLGID
                     VENGYTPKYITIKGKQKLVKELKAEAKKCDGVLLATDPDREGEAISWHLANILGLDPA
                     APNRVTFDEITKKGVKEGMAHPRAINIDLFNAQQARRELDRLVGYKLSPFLWRKVRKG
                     LSAGRVQSVAVRLIRDREIEIENFKPDEYWNIDALLHAQGDKSEFTARLAATADGKKL
                     TVTNKAQADGILAALDGKDYTITKIEKGKRRRQPAPPFITSTLQQDASRAFGFSATRT
                     MRAAQTLYEGVDIAGHGTVGLITYMRTDSLRIAAEAQAAAKTFIADRWGENYVCKTAR
                     KWKSRSATAAQDAHEAIRPSMPELTPDEVEQSISGDTAKLYRLIWSRFMASQMADCIQ
                     DTVSASITAGEYLFRASGFRVAFDGFTALYEESTDDTKKKETALPPLEEGQKLALKKL
                     TADQKFTQPPPLYTEATLIHALEENGIGRPSTYAPIITTIVDRGYVEKDQKKLKTTPL
                     GQAVNTVMMEQFPNIVDVKFSADMEKKLDVVEAGQADWVKTIDDFYQGFSKSLEQAEK
                     NMEGKRIKVEDIPTDEICEKCGRPMVIKSGRYGKFVACSGFPECRNAHPLVKDTGGLC
                     PLDGGHMLVRKSAKGRVYYGCSNYPKCNYMTWDEPVPEKCPQCGSTLFKKKGQLYCAK
                     EGCGFVKAIEKK"
     gene            complement(9187..10287)
                     /locus_tag="ACZPAX_01860"
     CDS             complement(9187..10287)
                     /locus_tag="ACZPAX_01860"
                     /inference="COORDINATES: protein motif:HMM:NF014533.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HIR1112676.1"
                     /translation="MDKTLAWLWLADAVGSGCQYARELLDLYPDPAELYDILRVGTEA
                     PPACLSAHALAQLRDTTPFDYEERLDHCLLSGIDILTPDEGTYPDRLRDLPDLPLVLY
                     VTGSTACLNGGRYAGMVGTRRPSAYGRQAAFDLSLAMAQAGVVLVSGMADGLDSEAHR
                     AAVQAEVPTVAFLGTAIDKTYPAANAKLRTAIEKGGGAVCSEYPPGYTGRTTGTFLAR
                     NRLIAAQSEVLCVAEARTRSGTLNTVGHAERLGRPVLAVPGSIYSALSEGTNELLCTH
                     RAEPLCKAADALDILGIGAETADPAQQRFDPAAVSPDAQAVYAVLKPTPQGIDALCAA
                     ARLPAGRVLAACTELELMGGAQAQPGRRYIAL"
     gene            complement(10341..11108)
                     /locus_tag="ACZPAX_01865"
     CDS             complement(10341..11108)
                     /locus_tag="ACZPAX_01865"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HIR1112677.1"
                     /translation="METITSRDNAKIKYACAVRDTEKQRAADGVFFAEGPKLCLELAK
                     SCTPRVVYATAAALAKTPELARFDPAEIAPHVAEKLSGTKSNQGVFALFETPVPPADI
                     LNTARRILALEGVQDPGNVGTLLRSAAAFGFDAVVLGPGCASPLSPKTLRSSMGAAGR
                     LPTLHTEDLPAALQKLRARGITTLATALYRSRPLDEAGNDFPGGLCVVIGSEGQGLTD
                     ATVEACDMAVRIPMTDRVESLNAAVAGSVLLWHFRGV"
     gene            complement(11385..11738)
                     /gene="rplT"
                     /locus_tag="ACZPAX_01870"
     CDS             complement(11385..11738)
                     /gene="rplT"
                     /locus_tag="ACZPAX_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009984034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HIR1112678.1"
                     /translation="MARIKGATMTHKRRAKTLKLAKGYYGAKSKHFRMAKQAVMKSGN
                     YAFVGRKQKKRQFRNLWITRINNAVRAQGMNYSTFMNGLKKAGVELNRKMLSEMAIHD
                     PASFNALVETAKKAL"
     gene            complement(11797..12003)
                     /gene="rpmI"
                     /locus_tag="ACZPAX_01875"
     CDS             complement(11797..12003)
                     /gene="rpmI"
                     /locus_tag="ACZPAX_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HIR1112679.1"
                     /translation="MAKMKLKTLSGAKKRFKMTASGKIKRNASKRRHILTKKTTKLTR
                     GLRMPKYVDKTNEAAVKKMMPYGG"
     gene            complement(12033..12632)
                     /gene="infC"
                     /locus_tag="ACZPAX_01880"
     CDS             complement(12033..12632)
                     /gene="infC"
                     /locus_tag="ACZPAX_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005935558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HIR1112680.1"
                     /translation="MFGGCFYGSRFYHLSYTCGRCVTIASNSKSNELEINEQIRDKEI
                     RLIGADGAQMGIMSPRDALKMAIDKDLDLVKVAPQAKPPVCKILDYGKYRFEMQKKEK
                     EAKKNQKVVELKEIRLSLNIDTNDFNTKVNQAAKFLQQGHKLKVSIRFRGREMAHTSL
                     GLDVHKRFAEALVGKAVIDKQPKLEGRSMMMFMSPVPNK"
     gene            complement(12954..>13782)
                     /locus_tag="ACZPAX_01885"
     CDS             complement(12954..>13782)
                     /locus_tag="ACZPAX_01885"
                     /inference="COORDINATES: protein motif:HMM:NF024908.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1112681.1"
                     /translation="VGVSVTAVRQWLRIYEAEGPDGLAPRTTSRHLTAEEKQAAVAAY
                     LAGEGSLFAICKKYGIRSTQNLQKMVETVQAGGTLRGYHGASRHKGTAYTTAADREKI
                     VRECLQNGCDYGVAALKYNVDYKALVRWVGQYRAGGAAALHDARHSSEGTSRAKGRER
                     TVPPMQRLVIVLDCLENEKNYGAMAQKYAVAYHQVYRWVQRYLRDGWRGMNDKRGPTD
                     DPGPEREWARVAPANEDAAEKWVARCAGVPDALPALLRRAGCSWCVIAKILFDLARM"
BASE COUNT         2189 a         4267 c         4253 g         3073 t
ORIGIN      
        1 gcagctgcta agccaaaagc gcagataata ctttgtgtat tatcgagcat tttggcaacg
       61 cagatgccgt gccgcagccg ccggagcggt gcttaagccg ttaggcggga attgtgcggt
      121 gttgccttag gtaatcaaat ccgcactgac attatccaga tccagccgca gaatcttcga
      181 tacgccgtct tcctgcatgg tgacgccgta caaaacatcg gcggcttcca ttgttccgcg
      241 gcggtgggtg atgacgatga actgggtgtg gtcggtcatg cggcgcaggt attgtgcgta
      301 ccgcgcgacg ttgacgtcgt ccagtgcggc ctcgatctca tccaggaagc agaacggcgc
      361 ggggttgacg ttgaggatgg caaaatagat cgatatagcg acaagtgcct gctcgccgcc
      421 cgaaagagca gacaggtttt tgatgacctt gcccgggggc gagacctcga tctcgatgcc
      481 ggattccagc acgttcgcct cgtcggacag gtagagccgc gcgtggccgc cgccgaacag
      541 ttcccggaaa atctgctgga agtgggtgtt gatctgcttg aagctcgtcg tgaacagctc
      601 ttccatctcg ctgcacagct cggcgatcat cttggtcagt tcgctcttgg ccttttccac
      661 gtcggtgacc tgggctttca ggaagtcata gcgctccttg acctctttgt attcctcgat
      721 cgcgccaacg ttgacattgc ccaagccgcg aattttgccg cgcacctcag tcacgctgcg
      781 gcgcagctcg gtcaggctct cgaagggcac acactggctg cgggcttcgc tctcggtcag
      841 ctggtattcc tcccacagct tgctgttggt gtcgttcagc tcggtctcgg cggcggtgcg
      901 gcgctcggcc aggcgggcca tttcgccgct catacgctct cgctcgtcgg tcagggcacg
      961 gttgtcctgg cccaatttgg cgatggcggc ttccttctcc atgcgggccg cattggcgga
     1021 acggatggtt tcctccgccg ctgcgattct ctgctggttt tcgccgcggg tgcgctccat
     1081 ctcggcgatt ttcaaggtgt tcgcctcgat tttcgccttg gccgcgtcga tggcggcgtg
     1141 cagctcccgg gcgcgggcct cggcctcgcc ggtacggctc tgcaagccct ccaaagcggc
     1201 ctcatgcagg ctgatgtcct tctcgtcggc cagtcgctgc atctgctttt cagagagctc
     1261 gccggtgatc ttttcgcgtt ctgccgtgag acttccggtg ctttccccca gagccgccag
     1321 ctcggcggtg taccggacca tctcggcttc ggcgtcggcc tgggcctttt ctgcctcagc
     1381 gcgggctgtc tcgttctggg tcacggcggc ctgctggttc gcaatttcgg cttcgatgtt
     1441 gcgggccgtc tcctcgtact gggcggcagc tttcgtcagg cgttccagtt ccagcccggc
     1501 gcggacgtgt tcggtggcgg cgttcatgcc ctcgccctcg gcacccgcca gctgggcact
     1561 gaggttatct acctcggcct tgcgggcggc cagttccttt tcggcttcgg tgcgcttttc
     1621 ttccagcttt ttcagccggg tgcgcagctc gtccagctcc tgtttgcggc tgaacacgcc
     1681 gacgctgcgg gccgtggagc cgccggtgaa ggagccgccc gcgttgatga cctggccgtc
     1741 cagcgtgacg atgcggttgc ggtagccgag gttccgcgcc acggcggagg cctcggtcag
     1801 gtcctcgacc acgatgatgc ggcccagtaa attgtcgatg atatgctgat attttgggtt
     1861 tgtcttaacg agatcggcgg cgacttccgc cgtgccggtc aggcggcctg taaagcggct
     1921 gccctgcacg gtgtccagcg gcagaaacgt cgcgcggcct gcgcgctcgt ccttcaaaaa
     1981 gccgatggcg gcgcgtgcac agccctgatc ctcgaccaca atgttttgca gcgcaaagcc
     2041 gagggccgtc tcgatggctg tctcatagcc tttttcgacg gtgatgatgc ccgccaccgg
     2101 gccgatgatg ccccgcagac gcccgccgcc tgcggcacgc atgacggcct tgacgctctg
     2161 ctggtagccg tccatgttgc gctccagatc ctctaaaata tggatgcgct gggcagcagc
     2221 ggatttctcg cggtcagcct tttgcaggtt gtcggcagct tcggcctgct ggcggcggcg
     2281 gctgtcgagc ttgagtttca ggcccgcctt gatgttgtcg ttgcgggtca cggcctcctg
     2341 ggcgtctttg aagcggcgct cggcgcggcg gcgttcctcg tcggtggcag cagcgtcggt
     2401 ctgcaaagtc tcggcctgct gcctggcttc ggccaggcgg ttggcggcgg cctcccctgc
     2461 cgactgtgcc gacgcggcgc ggaccttggc ggcggtggcg gtgtcctgca ggcgggccat
     2521 agcggcgtca atgacgtcgc ggcgctcgcc gcttgcggcg gccttttctt ccagcgcgtg
     2581 cagggcctcg cgcagctcgg ccacgcgggt gtccagcgca gccacgccgt cctttaaagc
     2641 gtcgatggcg gcgcggtggt cggcggcctc gcggtcaatg ctttcccggc cctggcccgc
     2701 gcgttccagc tcggtatttg cctcgtcgat gcggaaccgg ctgtgctcgc tctcgttttt
     2761 caagacggcg atctcgctct cgctgccggc gttggcctcg gtaatggcgc ggatatcggc
     2821 attggcctgc tcgactttca gcatgagctg ctgcgtttct tcgcgcagat ccgcgttttt
     2881 ttcatcaaag gcgtccagct ggcggctcag acggtcgtag tcggcctctg cggcctcgta
     2941 cttgcgctgc tgatcgcgca gtgtatcgtt ggcgcggcgg atggcatcca cccacagcgt
     3001 aatttccaga ctcttgcgct tctcgctcaa ttccagaaac tgctgggcct tctcgctgtc
     3061 gcgcttcagc gggccgacgc gcttttccag ttcgcccaaa atgtcgcgca ggcgctctaa
     3121 gttgccctcg gcggcgtcca ggcggcgctc ggcctcattt ttacggtagc ggtatttggc
     3181 gatgccggag gcttcctcaa aaatttcacg gcgctctgcg gattttgcgc caacgatctc
     3241 ggcaatgcgg ccctggccga cgatggcgta gccgtcacgg ccaatgccgg tgtccagcag
     3301 cagctcgtac acgtccttta agcggacgct ctggccgttg atggagtatt cactttcgcc
     3361 gctccggtag tagcggcggc cgatggtgac ttcgtcggcg tccatgtcca ggccgtggtc
     3421 gctgttgtcg atggtcagtg cgaccgacgc atagcccatg gcaccgcggg tctgggtgcc
     3481 gccgaagatg acgtcctcca tcttgcccga accgcgcagc tgtttggagc tggtctcgcc
     3541 cagcacccag cggatggagt cggagatatt cgatttgccc gagccgttgg gtccgacaac
     3601 gccggtaatg ccctgcccga tcgagatctt cgtcttatcg gggaaggact taaagccttg
     3661 gatttccagt tctttcaggc gcattgaagt agtgttacct ttcgattctg tctggtcgta
     3721 ggggccgggc atgcccggcc cctacagagc atcattaaac ggcaatatta ctgcagtttt
     3781 tccagcgcca cttttgcggc gtgctgttcg gcggctttct tgctgcggcc tttgccctcg
     3841 gccaaaagcc ggccgttgcg ccagacgcag acggtgaact gcttgtcgtg gtcggggccg
     3901 gtctcgccgg tgacttcgta tttcagcacg gcgctggggt cctgctgcac gacctcctgc
     3961 aaacgggtct tgtagtcgtc ctcggcggtc ttgccctcgg tgatgaaggg caggatgaag
     4021 ttgcgggcgg tctccatgcc gccgtccaga tacagtgcgg caatgacggc ctcgaacgcg
     4081 tcagacacga cgctgggccg gttacggccg ccgcccaggt cctcgccgtg gcccaggcgc
     4141 aggtactcgc ccaggtggat ctcctgggca aactggaaca gcgcgtcctc gctgacaagg
     4201 cttgcgcgca tccgggtcaa ttcgccctcg gggcggccgg actggtggaa caggtagtcc
     4261 gccacgacga cggacagcac gctgtcgccc agaaattcca gccgctcgtt gtgggtggta
     4321 gcgaccttgc tctcattggc aaagctggtg tgggtcaatg caatgcgcag cagcgcagga
     4381 tttttgaatg tatagtgcag gacttcctgc aattcttccg gcttgtgagc cataattatc
     4441 acctttccgg cttttgtagg gtcgtagggg ccgggcatgc ccggccctca accttgcctt
     4501 gctgtcccat gaaggggtta tcatttttat gctgcgggcc gggcatgccc ggcccctaca
     4561 aggcggcttt acttttaagc gtctgcttct tcttccttct tcgcggccac ctcgcccagc
     4621 gccttctcca tcgtgccgca caggtcgttg gctacacaga gcttggcctg gcggatggcg
     4681 ttcttgatgc ccttggcgtg gctggaacca tgagccttga tgaccggctt ggccgcgccc
     4741 agcagcggtg cgccgccgac ttcctcggag tccatcatcc gcttgagttc acccaggccg
     4801 cccttgatgc ccaggtagct gagcttcgtg atgaggttct gcaggaagat ttttttcagc
     4861 atccccagca gcgttttggc aacgccctca gtcaatttca agaccacatt accgacaaag
     4921 ccgtcgcaga ccacgacgtc cacctcgccg tccatgatgt agcgcggctc gatgttgccg
     4981 gcaaaatgga tgcaggggtt cgctttcagc agctggtgcg cctcgcggta ggtgggggtg
     5041 cccttggtgt cctcagcgcc gttgttgacc agtgcgactt tgggcgctgt gcggcccatg
     5101 accttttccg cgtagacgct gcccatcgtg ccgaaagcat tcagcatctc ggggcggcac
     5161 tcgacgttcg cgccgcagtc cagcagcagg aaattctgct ttgcgccggg catcggggtg
     5221 gccagtgccg ggcgcttgac gcccttgacg gtgcgcacga tgaggctggt gccgacgtgc
     5281 agcgcaccgg tggaaccggc ggagacaaag gcatcgccct ctccgttttt cagcatggtc
     5341 aggcccttca caaggctgga atccttcttg tggcgcacag ccttgaccgg gtcgtcgtgc
     5401 atatcgatgt tttcggtgca gttgatgatt tcaaacggtg caaggtcgat tttattttct
     5461 gccgcgcagt cgcggatggc tttttcctcg cccagcagcg tgagagtcat gccgtcgccg
     5521 aactctgcag ccgcctggct tgcaccctgc aatacacaca gcggggcgtt gtcaccgccc
     5581 atgacatcca ctaaaatttt catttgtatt cctccgtcca tgcacgcatg gcatcttggg
     5641 cgttctgcga gagcgccatg tagcgctccc gcttatcgcg gatagcgttg atctcctcgg
     5701 tgcggggcag gatgcccatg tttgcaccca tcggctggaa gtctttgttc ggcgtcgtga
     5761 tgtagtcgag cagtgcgccg cacatcgtcg ccgccggggg cggcggcagt tcgcatcctt
     5821 gcaagcgggc caaaatctgg tgggccgcca gcaggccgct ggccgcgctc tccatatagc
     5881 cctcaaagcc ggtgatctgc ccggcaaaga acacgttggg gtggtcagcc aggaattgct
     5941 gcttggtcag caccttgggg ctttccaaaa acgtgttgcg gtgcatgacg ccatagcgca
     6001 caaactcggc gtgttccagg ccggggatca tgctgaacac ccgcttctgc tcaccccact
     6061 ttaagttcgt ctggaaccca acgaggttgt agagcgtgcg ggccgtgttt tctgcccgca
     6121 gctgcacggc ggcccagggg cggtggccgg tgcgcgggtc ccgcaggccg acgggtcgca
     6181 acggcccaaa gcggatcgta tccgcgccgc gggcggccat gacctcaatc ggcatacagc
     6241 cctcgtagac ggtcaggtct ccgtcaaagt cgtggagcgg cgcacgctcg gcggcgatga
     6301 gcgccgcgtg gaagttctca tactcttcct tattaaaggg gcagttgagg tagtcggcct
     6361 cgccgcggcc atagcgggat gcagcaaaga ccttgtcata gtccaggctc tcggcggtca
     6421 cgatgggcgc aacggcgtcg tagaaactca gccgatggtc gccggtcagc gccgccacgg
     6481 cctgggccag cgccccctcg gtcagcgggc cgctggcgac gagcaccggg gcgctctcgt
     6541 ccagagccgt gacttcctcg cgatggaccg tgatgttcgg gtgcttttcc accatttccg
     6601 tcacggcggt ggaaaacaca tcgcggtcga cggccagcgc accgcctgcc gccacgcggg
     6661 cggtctcggc ggcgtccaac aggtggctgc ccatcatctt catctcgatt ttgagcaggc
     6721 cggatgcgga atccgggcgc tcggctttca aggagttaga gcagatgagt tccgcaaacc
     6781 cctcgctttt atgcgcgggg gaaaattttg tgggtttctg ctcgtacagg tcgacctgca
     6841 cgccgcggtc cgccagccac aacgccgcct cgcaccccgc caggccggca ccgataatgt
     6901 taagttgcat acttgtcctc tctcaggctc ccctttgcgg ggaggcttta aagcggtcat
     6961 tttttctcaa tcgccttcac aaacccgcag ccctctttgg cgcagtacag ctggcccttc
     7021 tttttaaaca gggtgctgcc gcactggggg catttttcgg gcacaggctc gtcccaggtc
     7081 atatagttgc acttggggta gttgctgcag ccgtagtaca cgcggccctt ggcgctcttg
     7141 cgcaccagca tatggccgcc gtccagtggg cacaggccgc cagtgtcctt gaccagcggg
     7201 tgggcgttgc ggcactcggg gaagcccgag cacgccacaa acttgccgta ccggcccgat
     7261 ttgatgacca tggggcggcc gcatttttcg cagatctcat cggtcgggat atcctcgacc
     7321 ttgatgcgct tgccctccat gttcttctcg gcctgttcca ggctcttgga aaatccctgg
     7381 tagaaatcgt cgatggtctt gacccaatcg gcctgaccag cttcgacgac gtcgagcttt
     7441 ttctccatgt cagcgctgaa tttcacgtcc acgatattcg ggaactgctc catcatgacc
     7501 gtgttgacgg cctggcccag cggggtggtt ttcagctttt tctggtcctt ctccacatag
     7561 ccgcggtcga cgatggtcgt gatgatgggc gcgtaggtgg acgggcggcc gatgccattt
     7621 tcctccagcg catggatgag cgtcgcctcg gtgtagagcg gcggcggctg ggtgaatttc
     7681 tggtccgccg tcagtttttt cagcgccagt ttctggccct cttccaacgg gggcagggcc
     7741 gtttccttct tcttcgtgtc gtcggtggat tcctcgtaca gcgcggtgaa gccgtcgaac
     7801 gcgacccgga agcccgacgc gcggaacagg tactcccccg ccgtgatgga tgcggacacc
     7861 gtatcctgga tgcagtcggc catctggctt gccataaagc ggctccaaat caggcggtaa
     7921 agcttggcgg tgtcgccgct gatgctctgc tcgacctcgt cgggtgtcag ctccggcata
     7981 gacggacgaa tggcttcgtg ggcgtcctgg gcggcggtgg cggaacggct cttccacttg
     8041 cgggcagtct tgcagacgta attctcgccc cagcggtccg caataaaggt cttggccgcg
     8101 gcctgggcct cggcggcgat gcgcagagag tcagtacgca tataggtgat aaggcccaca
     8161 gtgccgtggc ccgcgatgtc cacgccttcg tacaatgtct gggcagcgcg catcgtgcgg
     8221 gtggccgaga agccaaacgc gcggctggcg tcctgctgca aagtcgaggt gataaagggc
     8281 ggcgcgggct ggcggcggcg cttgcctttt tcaattttgg taatggtgta atccttgccg
     8341 tccagcgcgg ccaggatgcc gtcggcctgg gccttgttcg tcaccgtcag ctttttgccg
     8401 tcggcggtgg cggccaggcg ggcggtgaac tcactcttgt cgccctgggc gtggagcagc
     8461 gcgtcgatgt tccaatactc gtcgggcttg aaattctcaa tttcaatttc ccggtcgcgg
     8521 atcaggcgca cagccacact ctgcacgcgg cctgcggaca ggcccttgcg gaccttgcgc
     8581 cacaaaaacg ggctgagctt gtagcccacc aggcgatcca gctcgcgccg ggcctgctgg
     8641 gcgttgaaca ggtcaatgtt gatggcgcgg gggtgggcca tgccctcctt gacgcctttt
     8701 ttggtgattt catcaaaggt cacgcggttg ggcgcggcag gatccagccc cagaatgttc
     8761 gccaggtgcc agctgatggc ctcgccctcg cggtccgggt cggttgccag cagcacgccg
     8821 tcgcactttt tggcttcggc cttcagctct ttgacgagtt tctgcttgcc cttgatggtg
     8881 atatatttcg gtgtatagcc attttcgaca tcaatgccca gcgtgctggc gggcaggtcg
     8941 cgcacatggc ccatgctggc tgtgacctta tagccacggc ccagatactt gccaatggtt
     9001 ttcgccttgg caggggattc cacgatgact aatttcggca taagctacct caatgaaaaa
     9061 gtattttaga taatagataa gagataaaag ataaaaaata ttgtggatcg caggccgttg
     9121 gcctgctcaa aattgtgcgc tgtgcgcact ccttttttta ttatctatta tcttttatct
     9181 attatcttat agcgcaatat atctgcgtcc cggctgggcc tgtgcaccgc ccataagttc
     9241 cagctccgta caggccgcca gcacgcggcc ggcgggcagg cgggcggcgg cacacagggc
     9301 gtcgatgccc tggggcgtgg gcttcaaaac ggcgtagacc gcctgcgcgt cggggctgac
     9361 ggcggcgggg tcaaaccgct gctgtgccgg gtcggcggtc tctgcgccaa tccccagtat
     9421 gtccagcgcg tcggcggctt tgcacagcgg ctcggcgcgg tgggtacaca gcagttcgtt
     9481 cgtgccctcg ctcaacgcac tgtaaatgct gccgggcacg gccagcaccg ggcggcccag
     9541 ccgctcggcg tggcccacgg tgttcagcgt accgctgcgg gtgcgggcct cggccacgca
     9601 gagtacctcg ctctgggcgg caatcagccg attgcgggcc aagaatgtgc cggtcgtgcg
     9661 gcctgtgtag ccgggcgggt actcgctgca gaccgcaccg ccgccctttt cgatggcggt
     9721 gcgcagcttg gcgttggcgg cggggtaggt cttatcgatg gccgtgccca gaaacgccac
     9781 ggtgggcacc tccgcctgca cggcggcgcg gtgggcttcg ctgtccaggc cgtccgccat
     9841 gccgctgacc agcaccacgc ccgcctgtgc cattgccaga gacaggtcaa acgccgcctg
     9901 ccgcccgtag gcgctggggc ggcgggtgcc caccatgccc gcgtaccgcc cgccgttcag
     9961 gcaggcagtg ctgccggtca cgtaaagtac cagcggcaaa tcgggcagat cacgcaagcg
    10021 gtccgggtag gtgccctcgt cgggcgtcag gatatcgatg cccgacaaca ggcagtggtc
    10081 gagccgttcc tcatagtcaa acggcgtcgt atcccgcagc tgtgccagcg cgtgggcgct
    10141 caaacaggcg ggcggggcct cggtcccgac acgcaggata tcgtacagtt ccgccgggtc
    10201 cgggtacagg tccagcagtt cccgcgcata ttggcacccc gagccgacgg catccgccag
    10261 ccagagccaa gccagtgttt tgtccatggc ggatgccccc ttttattctc tcggctgcat
    10321 agcggcgggc agaggactgc ttacacgccc ctgaaatgcc acaacagcac gctgcccgcc
    10381 acggcggcgt tcaaactctc gacacggtcg gtcatcggga tgcgcacggc catgtcgcag
    10441 gcttcgacgg tggcatccgt caggccctgg ccctcgctgc cgatgacgac gcacagcccg
    10501 cccgggaagt cgtttccggc ctcgtccagc gggcgggaac ggtacagcgc cgtcgccagc
    10561 gtcgtgatgc cccgggcacg cagcttttgc agcgccgcgg gtaaatcctc ggtgtgcagc
    10621 gtcggcagcc gccctgccgc gcccatcgag gaccgcagcg ttttgggcga caaaggcgac
    10681 gcgcagcccg gtcccagcac gacggcgtca aagccgaagg ccgcggcgct gcgcagcagc
    10741 gtacccacgt tgccggggtc ctgcacgccc tccagcgcca ggatgcggcg ggccgtgttc
    10801 aaaatgtcag cgggcggcac gggcgtctca aacagcgcaa acacgccctg gttggacttg
    10861 gtaccgctga gcttttccgc cacatggggc gcgatctcgg cggggtcgaa tcttgccagt
    10921 tccggcgttt tggccagcgc cgccgccgtg gcatacacca cgcgcggcgt gcagcttttc
    10981 gccagttcta ggcagagttt tggcccttcg gcaaagaaca cgccgtcggc ggcacgctgc
    11041 ttttcggtat cgcgcactgc acaggcgtat ttgatcttgg cgttatcgcg gctggtgatc
    11101 gtttccattg tagcgcccct tttgtataaa atcaactaaa ttgtgtgaat aaagagaaac
    11161 agcagtctgc cggaaggccg tccctcatcc gtcggcggtc ggggccgccg acaccttccc
    11221 ccgaagggga aggctccaca gcctcaagcc ttctcccctt gggggagaag gtggcccgca
    11281 ggccggatga ggggagagct ttccttggtt gcccgctttt ttgccatttt cccctttaaa
    11341 cacaaaagga ctcgcgcgtg tgcgcgagtc tttttagcaa gctattacag agccttcttg
    11401 gcggtctcga ccagcgcatt gaagctggcg ggatcgtgga tggccatctc agacagcatc
    11461 ttgcggttca gctcgacgcc ggccttcttc aggccgttca tgaaggtgga gtagttcata
    11521 ccctgagcac gaacagcgtt gttgatgcgg gtgatccaca ggttgcggaa ctggcgcttc
    11581 ttctgcttac ggccaacgaa agcgtagttg ccgctcttca taacggcctg cttggccatg
    11641 cggaagtgct tggacttagc accgtagtaa ccctttgcga gcttgagagt cttagcacga
    11701 cgcttgtgcg tcatggtagc gcctttaata cgagccatgt tttatatctc ctttgtctat
    11761 aactggtagt aaactgacct ttaaatgtcg tagcgattag ccaccgtagg gcatcatttt
    11821 cttgacagca gcctcgttgg tcttgtcaac gtacttgggc atacgcaggc cacgagtgag
    11881 cttggtggtc ttcttggtca gaatgtgacg gcgcttggac gcattgcgct tgatcttgcc
    11941 ggaagcagtc atcttgaagc gcttcttggc gccggaaagg gtcttgagct tcattttagc
    12001 cattgttgtt tcctcctaga gagtttactt tcttacttgt tgggaacggg ggacatgaac
    12061 atcatcatgc tgcggccctc aagcttgggc tgcttgtcga tgacggcctt gcccaccaac
    12121 gcctcggcaa aacgcttatg gacgtccaga cctaagctgg tatgcgccat ttcgcggccg
    12181 cggaaccgga tgctcacctt gagcttgtgg ccctgctgca ggaacttagc agcctgattc
    12241 accttggtgt tgaagtcgtt ggtgtcgatg ttcagagaca ggcggatttc cttgagttca
    12301 accactttct ggtttttctt ggcttccttt tcctttttct gcatctcgaa ccggtattta
    12361 ccgtaatcaa gaattttgca caccggcggc ttggcctgcg gtgcgacctt gaccagatca
    12421 aggtccttgt cgatagccat tttcagtgca tcgcgggggc tcataatgcc catctgtgca
    12481 ccgtccgcgc cgatcaagcg gatctctttg tcgcggatct gctcattgat ctccagttcg
    12541 ttggatttgc tgttgctggc gattgtaaca cacctcccac aagtgtatga taaatgataa
    12601 aagcggctgc cataaaagca gccgccgaac atacgaagtt gcgcacctag tgcgtatcat
    12661 ggtatgaccc ggggttttga caaaccctgc aaggtgagcc gacggcctac tgccggttgc
    12721 tgcttttttt acatcgatta gtttaccaca aaagcaaggg cgtgtcaata gggccgccga
    12781 aagttttttg cgtatttatt cctctcgtac agatgcacgg ccaaagcgcc gccgccctgt
    12841 aggggccgca tatatgcggc ccgcagcttt cccccagatg ccccgctgcc ttacacccgc
    12901 gggccgggca tgcccggccc ctacggctct acaatgtttt tcatcgtaca gcactacatc
    12961 ctcgccagat caaacaaaat ttttgcaatc acgcaccaac tgcacccggc gcggcgcagc
    13021 agggcgggca gggcgtccgg cacgcccgca caccgggcca cccatttttc tgcggcgtcc
    13081 tcattggcgg gggccacgcg cgcccactcc ctctccgggc cggggtcatc ggtggggccg
    13141 cgcttgtcgt tcatgccgcg ccagccgtcg cgcaggtagc gctgcaccca gcggtagacc
    13201 tggtggtagg ccacggcgta tttctgcgcc atagcgccgt aatttttctc gttttccagg
    13261 cagtccagca caatgaccag ccgctgcatc ggcggcacag tgcgctcacg ccccttggcg
    13321 cggcttgtgc cctcgctgct gtggcgggcg tcgtgcaaag cggccgcccc gcccgcgcgg
    13381 tactgcccca cccaccgtac aagcgcctta tagtccacgt tatattttaa cgcagccacg
    13441 ccgtaatcgc agccgttttg caggcattcc cgcacgatct tttcccggtc ggcggccgtg
    13501 gtgtaggccg tgcccttgtg gcgcgacgcc ccatggtagc cgcgcagtgt gccgcctgcc
    13561 tgcacagtct cgaccatttt ttgcaggttt tgggtactgc ggatgccgta ttttttgcag
    13621 atagcaaaca gcgagccttc ccccgccagg taggcggcga cggcggcctg tttttcctcg
    13681 gcggtcaggt ggcgggacgt cgtgcgcggg gccagaccgt ccgggccttc ggcctcatag
    13741 atgcgcagcc attggcgcac cgccgtgaca ctgacgccga cg
//
LOCUS       DBMDSQ010000026        13715 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_1380_length_13715_cov_2.330836, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000026 DBMDSQ010000000
VERSION     DBMDSQ010000026.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 13715)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 13715)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13715
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1380_length_13715_cov_2.330836"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            58..459
                     /locus_tag="ACZPAX_01890"
     CDS             58..459
                     /locus_tag="ACZPAX_01890"
                     /inference="COORDINATES: protein motif:HMM:NF012738.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR1112682.1"
                     /translation="MKTVTNLPFCMFMMMGYYSSVPRELEEAAQVDGCTLLGAVFRVL
                     IPVMLPSIVATGAYAFINAWNVYVYATAFITDKAKYTLPLALNVFQGEYGTDYGKLAA
                     GCVIALIPVVVIFAFIQKHLSGGATSGAVKG"
     gene            499..1887
                     /locus_tag="ACZPAX_01895"
     CDS             499..1887
                     /locus_tag="ACZPAX_01895"
                     /inference="COORDINATES: protein motif:HMM:NF024808.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR1112683.1"
                     /translation="MKATRTTAAVLSAAMAATLLAGCGGSSATSTAASTSTESTAEST
                     STAAAAPTGEKQTLEYWYHSADPVTDAYFEDYFDKINAEQDQYEIKYTSFSFADFQEK
                     FQMAVSTDTMPDVVSLGFSNVATFTAQDSLLPLDDYLDQIETLDKIDSSMVDSMRNIG
                     GGTLYGIPYAYNQEVAWYNTAKFKEKGIDAPPATQKEFLEDCEKYADPANSSYFYSLR
                     GVRPYDSLVAWLWTYTDGLGYDGSWFDENGKCILGDAKFAEALDTYANLYKNNEVSGD
                     SINNNFSQIVAEFGSGVSMYIIHNSSSESTHLTNLGEGNFAAARVLANDEGHYFASGL
                     QPNVYCITNQGDDHDYSGAVWLMNVLLDAECEGGMCQAIGRVPCNKDVEEQDWYKNDP
                     EMMLYASYLEDENYKQIQNPYWLTEFSTFISDDMTADFQAVLMGNMTAQDCVSGWAAK
                     IDEYQAAYEAQA"
     gene            1969..3981
                     /locus_tag="ACZPAX_01900"
     CDS             1969..3981
                     /locus_tag="ACZPAX_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-alpha-glucoside-1,2-lyase-like
                     domain-containing protein"
                     /protein_id="HIR1112684.1"
                     /translation="MADTVILFQDDFQSFPIGPLPFDREHSAMGEYHYSPLPGYRGQW
                     YDPIANCNYRGPSWLVTATDGVHYMEQTRVRNPLTHGVCPVLATGEERWQDYTFTVTL
                     RTLCSNEEAGVLFRYQTSLMHYAFFMNDNKVQFWRIEKKERTMLAEAPYEYNCDQYYT
                     MRVTCKGNTFTGSINGEKLLTVQDDRYTYGKVALAAFMPTQYTNVLITTTPEEAARIV
                     ECEDAEQARLAEKRSHYPRPKLYKVIDLKDFGASRQIRFGHLMGGDDWYFVMAQHQKR
                     VFKERYCNISCLTAVNVETGEVLWQVGEPSTLAVNQDTTCDLPFQIYDINNDGYDEVI
                     FARDFKVMILDGRTGKLLRSMDTPYNDDPADEILGIEFQKHAFDRLNVDAIRIVNVSG
                     KEHASDILIKDRYARLWVYDENFNLLWKFTHNNTGHFPYAYDFNGDGKDEIFSCYNMI
                     SSDGKLQWELPIDTDHTDEIVIGKFDPDIDEELLAIVSGWEGFMIVDKHGHILARDIN
                     GHGQRISVANYCPNMRGLQIATTTYWENQGIIYLYDCKGHEIWHMEPSSNGNVVAPVN
                     WKGDGTELILLNGNVKYGGMLDGDGDRVVLFPDDGHPDQCAEVLNLTGDPRDEIILWD
                     AHKMYIYTQDRPAPDGPVYHPEKYPEYSASNYRGEFSFAHWDKAGD"
     gene            4007..5224
                     /locus_tag="ACZPAX_01905"
     CDS             4007..5224
                     /locus_tag="ACZPAX_01905"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019113.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88/105 protein"
                     /protein_id="HIR1112685.1"
                     /translation="MLYERYLRAYAEQFRKTDRNMWNYEDGCVLIGLRALYQATGDAF
                     YFDVLNSFMERYIEPDGTIRRYVAEEYNLDYIPAGCALYTLYDETGESRYRKAIETLE
                     EQLRHQPRTPCGSFWHKGIYPNQVWLDGLYMGLPFHAAYALRFGDAATLDDIMLQFRN
                     ARKYLYDGKTGLYYHAWNETKDVFWADPKTGLSQNFWTRAIGWYLMAMADIYAMLPDS
                     RADLQKELAALWKEAIDGMLRWQEPDSGLFYQLPALPDVKGNYLETSGSLMVAYSLLK
                     GARLGVLAEPKYRAAGEKILVGIELRQFTVHDGKVELHGICKGAGLGPAGNLRRNGTV
                     EYYLSEDVVHDEQKGVGVCMMAYAEYLQAKQAGALTDDFPKVEIFNKGYDPIMPGDPA
                     FIAFEKNRVGKTG"
     gene            5230..6657
                     /locus_tag="ACZPAX_01910"
     CDS             5230..6657
                     /locus_tag="ACZPAX_01910"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HIR1112686.1"
                     /translation="MQFSIAKPEEVGVSSAALSQTLDYLAQRRVALHSLLVMRHDALI
                     CEGYAAPFTKDTLHRMYSETKSLVSLAVGVMCAKGYLDLDDHIVDHFAEKLPLGGPSP
                     ELARLTIRDMLRMATPYNGTTYKRRPTKDWVGSFFTARTDHEPGSFFCYDTSSSHTLC
                     ALVEKRMGRPLLDVLRREALDAIGFSEQSYCIRDPMGISQGGSGLMATPRDMLALLRL
                     VAQGGQWDGQQLLPQDYLQAATAWQIDNAENGGSGGWDFAQGYGYQFWRLTHNAWSMY
                     GMGGQLAICLPDQDMLLVTTADTQPLAGGVQTILDAFWNCLLPGVADAALPANPAAYA
                     ELTRKLSTMQLPIVENLAAPDTELCCATVQMGLNAPGLTALQLQENALVLHYGGKTCT
                     LPFRTGALVQSHLWDDPALPCVIAAGWRAPDSLLFRVHLLGERLGSLSLQLKLRPGGA
                     TLALCSHEEHPDPDFSGTAEGVTAV"
     gene            6936..7757
                     /gene="thiM"
                     /locus_tag="ACZPAX_01915"
     CDS             6936..7757
                     /gene="thiM"
                     /locus_tag="ACZPAX_01915"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005920416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HIR1112687.1"
                     /translation="MLGTCIENVRKNVPLVHNITNYVTVNDVANILLACGGSPIMSDE
                     PEDVEDITTICGGLNINIGTLNQRSIAGMFRAGARANALGHVVLLDPVGAGASALRTN
                     TAVELMEKVKFTVIRGNISEIKTLALGSGTTKGVDADVADAVTDANLDNAVAFVKDFA
                     AKSGAIVAVTGAIDLVSDGKTCYVIRNGRPEMGKITGTGCQLSGMMTAFVVANPDNRL
                     EAAAAAVCAMGLAGEIGWSRMQDGDGNSTYRNRIIDAIYNMDGATLDGGAKYELR"
     gene            7744..8385
                     /gene="thiE"
                     /locus_tag="ACZPAX_01920"
     CDS             7744..8385
                     /gene="thiE"
                     /locus_tag="ACZPAX_01920"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016324155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HIR1112688.1"
                     /translation="MNCDKKDLLLYAVTDRHWLDGRRLIDVVRESLDGGVTMVQLREK
                     TLEEGKFLEEAKELQALCRERGVPFLVNDNVEIAREMNADGVHVGQSDMEALDVRAIL
                     GPDKILGVSAQTVEQAVLAEKHGADYLGVGAVFPTGSKDDADDVSYDTLKAICQAVSI
                     PVVAIGGITQQNVEKLAGSGICGVAVISAIYAAKNIGQASKDLKAATEKMLHD"
     gene            8378..9022
                     /locus_tag="ACZPAX_01925"
     CDS             8378..9022
                     /locus_tag="ACZPAX_01925"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR1112689.1"
                     /translation="MTKGAIFDLDGVLLDSMGIWNDLGARYLRSRGLMPEPGLNEILF
                     SMSMEQGAEYLHTHYPLAQSAAEIEAGIETMLRDFYYNEVPAKPGAAALLAALAAKGI
                     PMAAATSSPRGHVTHALQRLGLLGYFAQIFTTGEVGVSKHEPTIYLLAAQALGSAPGE
                     TTVFEDSLYALKTAKAAGFYTVGVYDADGESDQAGLKAAADLYVKELGEAAGRW"
     gene            9138..9935
                     /gene="thiD"
                     /locus_tag="ACZPAX_01930"
     CDS             9138..9935
                     /gene="thiD"
                     /locus_tag="ACZPAX_01930"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008979896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HIR1112690.1"
                     /translation="MKTALTIAGSDSCGGAGIQADIKTMTANGVYAMSAITALTAQNT
                     TGVTDILESTPHFLAEQLDAVFTDITPDAVKIGMVSSAELIETIAAKLKQYGAKHIVV
                     DPVMVATSGSKLLRDDAVSALCANLLPMAEVLTPNIPEAEILADMPIKNAADMEKAAQ
                     TISEKYGCAVLCKGGHQINDADDLLWRGGAGKWFRGKRIDNPNTHGTGCTLSSAIASN
                     LAKGYDLDASVERAKAYISGALAAMLDLGRGSGPMDHMFALKGEFTE"
     gene            9932..11098
                     /gene="cytX"
                     /locus_tag="ACZPAX_01935"
     CDS             9932..11098
                     /gene="cytX"
                     /locus_tag="ACZPAX_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014117449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative hydroxymethylpyrimidine transporter
                     CytX"
                     /protein_id="HIR1112691.1"
                     /translation="MKKTSTFQNGLIWFGAGVSLAEILTGTSFAPLGMAKGFAAILIG
                     HIIGCAMMLLAGLIGGRTGRSAMETVKMSFGQKGGLLFAFLNVLQLVGWTAIMIYDGA
                     LAVGGIFAIGRWVWCLVIGALIVLWIAVGITDLGWINKITMAALFILTLVLCKVIFFS
                     GNAMVGVGGESLTFGAAVELAVAMPLSWLPLISDYTRDAEKPTQATWVSVLVYGAVSC
                     WMYVIGMGAAIFTGEYDIAVIMVKAGLGIAALVILVFSTVTTTFLDAWSAGISAESLS
                     AKLDGKKTAIVVTVIGTLGAILFPMDDITGFLYLIGSVFAPMIAVQIADHFILKRDRF
                     AAAADARNLVIWLVGFIAYRLLMGVDTPVGYTLPDMLLTVAICVIAEKVKPTKA"
     gene            11617..>13715
                     /locus_tag="ACZPAX_01940"
     CDS             11617..>13715
                     /locus_tag="ACZPAX_01940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1112692.1"
                     /translation="MTKKFSVLQRAAALLAALCLLAGLALPVCAEGTDDALSAQNVET
                     IQQGNDPTVETLPGEQTDEDKTAPDGAAAATPTPTPVATPTATPAPTPTAAPAATPTP
                     APTATSTATPTPTPTAMPTATPTATPTAKPTPKPTPTVTPIPDDEENSESEEKFVTFA
                     KVNAVAEGGKPVDGTTYTFYFAPPADWGEEVEFRGKRGGGNQDNDTEIIRPMSRVPGY
                     TASNKRPIYVVSLRYQEGNNGAECTHGGYVYIKFISKSGEHTIQMNGPNNWLSIDAMG
                     DHIFDAGGLEDKTYTTWNPNCLKPYAEVAQQIPYTNQKISFQNKTENELTNIKVTFYD
                     DKYVAFGKPQTIESLEAGKVSEGIEIPDDSCRYVGFTDASGKPIGKTYYNFYNDDVAS
                     EEVGTFQYNAETNYCFIYKGASDVQWGEPNTTRIYFDATFSDMSYSGDSGTTADGMPG
                     NDGKLYYILTGEDKTEQTGEMTRLEDRSESRHLWYVNAPDGYTKVQFSDTKTPPAGSD
                     INGRATDTLTWGNLEEPCFYADTGDDITYVGGYRGGYWGEKSDIRDAEAGKKKVDQNR
                     EPVVNLEKKEFTQQANTKYISTTLYDYYTDYELNGNNRAKNNCTEYSNQRSYVTFEQF
                     DRALSDYYSEYATEKNTPIQYPLYTGHFQPSNLGDNNRFANIANNLGLYGWGLKLGER
                     IIVPPKYRKILPPVGYY"
BASE COUNT         3027 a         4052 c         4018 g         2618 t
ORIGIN      
        1 tcctgcagga tgctgacgac atctttatcc agggcttgcg cgactggaag gtgaaagatg
       61 aaaacggtaa cgaaccttcc cttctgtatg ttcatgatga tgggttatta cagctcagtc
      121 ccgcgtgaac tggaagaggc cgcccaagtg gacggctgca ccctgctggg ggcggtgttc
      181 cgcgtcctga tcccggtcat gctgcccagc atcgttgcca ccggtgcata cgcattcatc
      241 aatgcgtgga acgtttacgt ctacgccacc gcgttcatca ccgataaagc caagtacacc
      301 ctgcccctgg cgctgaacgt cttccagggc gaatacggca ccgactacgg caagctcgcc
      361 gccggatgcg tcatcgcact gatccccgtt gttgtaattt ttgccttcat ccagaagcac
      421 ctctccggcg gcgcgacctc cggtgcggtc aagggataag gcgtagaatt agatttcatt
      481 tataggagga aaacacaaat gaaagcaacc cgtaccaccg ctgcggtcct gagcgctgcc
      541 atggccgcta ccctgctggc cggctgcggc ggaagctccg ccaccagcac cgccgcctcg
      601 acctcgaccg agagcactgc cgaaagcacc tctaccgctg cggctgcccc caccggcgag
      661 aaacagaccc tggagtattg gtatcactcc gctgaccctg tgaccgacgc ttattttgag
      721 gattatttcg acaaaatcaa cgccgagcag gaccagtacg agatcaagta caccagcttc
      781 tccttcgccg acttccagga gaagttccag atggctgtct ccaccgatac gatgcccgac
      841 gtcgtcagcc tgggcttctc caacgtggcg accttcacag cacaggattc cctgctgccg
      901 ctggatgatt acctcgacca gatcgagacc ctcgacaaaa tcgacagcag catggtagac
      961 agtatgcgca acatcggcgg cggcacgctg tacggcatcc cctatgcata caaccaggag
     1021 gttgcctggt acaataccgc caagttcaag gagaagggca tcgatgctcc gcccgctacc
     1081 cagaaggagt tcctggagga ctgcgagaag tatgctgacc ccgcaaactc ctcttacttc
     1141 tactctctgc gcggcgtgcg tccttacgac agcctggttg cctggctgtg gacctacacc
     1201 gacggcctgg gctatgacgg ctcctggttc gacgagaacg gcaagtgcat cctgggcgac
     1261 gccaagttcg ccgaagccct ggacacctac gccaacctct acaagaacaa cgaagtctcc
     1321 ggcgactcca tcaacaacaa cttctcccag atcgttgcag agttcggttc cggcgtctcc
     1381 atgtacatca tccacaactc ttcttccgag tctacccacc tgaccaactt gggtgagggc
     1441 aacttcgcag ccgcccgcgt gctggccaac gatgagggcc actacttcgc ctccggtctg
     1501 cagcccaacg tctattgcat caccaaccag ggcgacgacc acgactactc cggtgctgtt
     1561 tggctgatga acgtgctgct ggacgccgag tgcgagggcg gcatgtgcca ggctatcggc
     1621 cgtgtgccct gcaacaagga cgttgaagag caggattggt acaagaatga ccccgagatg
     1681 atgctgtacg ccagctacct ggaggacgaa aactacaagc agatccagaa cccgtactgg
     1741 ctgactgagt tctccacctt catctccgac gatatgaccg ctgacttcca ggccgttctg
     1801 atgggcaaca tgaccgctca ggattgcgtc tccggttggg ccgccaagat cgacgagtat
     1861 caggctgcct acgaagcaca ggcataattg aacatcggcc gcaagtaaag gagcatgaaa
     1921 aagcctttat ttgcggcttt tttagagaaa atcaataggg aggttgccat ggcagataca
     1981 gttattttat tccaggatga tttccagtct ttcccgatcg gtccgctgcc ctttgaccgc
     2041 gaacattccg caatgggtga gtatcattac agcccgctgc caggttaccg agggcagtgg
     2101 tatgacccca tcgcaaactg caactatcgc ggccccagct ggctcgtcac cgccaccgac
     2161 ggcgtacact acatggagca gacccgcgtg cgcaatccgc tgacccacgg cgtctgcccg
     2221 gtgctggcta ccggcgagga gcgctggcag gactacacct tcactgtcac actgcgcacc
     2281 ctttgcagca acgaggaagc cggtgtgctg ttccgctacc agacgtcgct gatgcactac
     2341 gccttcttta tgaatgacaa caaggtgcag ttctggcgca tcgagaaaaa agagcgcacg
     2401 atgctggccg aggcccccta cgagtacaac tgcgaccagt actacacgat gcgcgtgacc
     2461 tgtaagggca ataccttcac cggatccatc aacggcgaaa agctgctgac tgtgcaggac
     2521 gaccgctaca cctacggcaa ggtggcgctg gcggccttta tgcccaccca gtacacgaac
     2581 gtgctcatca ccaccacgcc ggaggaggcc gcccgcatcg tcgagtgcga ggatgccgag
     2641 caggcccgcc tggccgaaaa gcgcagccac tacccccgcc ccaagctgta caaggtcatc
     2701 gacctcaagg acttcggagc gtcgcggcag attcgcttcg gccatctgat gggcggcgac
     2761 gactggtact tcgtcatggc acagcaccag aagcgcgtgt tcaaggagcg ctactgcaac
     2821 atcagctgcc tgacagccgt caatgtcgag accggcgagg tgctgtggca ggtgggtgag
     2881 ccttcgaccc tggcggtgaa ccaggacacg acctgcgacc tgccgttcca aatctacgat
     2941 atcaacaacg atggctacga cgaggtcatt tttgcccgag atttcaaggt tatgatcctg
     3001 gacggccgca ccggtaagct gctgcgcagc atggatacgc cctacaacga cgacccggcg
     3061 gatgagattc tgggcatcga gttccagaag cacgcatttg accggctgaa cgttgatgct
     3121 atccgcattg tgaacgtctc cggcaaggag catgccagcg atatcctcat caaggaccgt
     3181 tacgcccgcc tgtgggtgta cgacgagaac ttcaacctac tgtggaagtt cacccacaac
     3241 aacaccggcc acttccccta tgcctacgac ttcaacggcg acggcaagga tgaaattttc
     3301 agctgctaca acatgatcag cagcgacggc aaattgcagt gggagctgcc catcgacacc
     3361 gatcacacgg atgaaatcgt cattggcaag tttgaccctg atatcgatga ggagctgctg
     3421 gctattgtct ccggctggga gggtttcatg atcgtggaca agcacggtca catcctggcc
     3481 cgggatatca acggccacgg ccagcgcatc agcgtagcca actactgccc gaatatgcgc
     3541 ggtctgcaga ttgccaccac cacctactgg gagaaccagg gcatcatcta cctgtacgac
     3601 tgcaagggcc atgagatttg gcacatggag ccttcctcca acggtaatgt tgtggcgcct
     3661 gtcaactgga agggcgacgg taccgagctg attttgctca acggcaacgt caagtacggc
     3721 ggcatgctgg acggtgacgg cgaccgggtc gtgctcttcc ccgacgacgg ccaccccgac
     3781 cagtgcgccg aggtgctgaa cctgaccggc gacccgcggg atgaaatcat cctgtgggat
     3841 gcccacaaga tgtacattta tacccaggac cgccccgcac ccgatgggcc ggtctatcac
     3901 ccggaaaaat accccgagta cagtgcctcc aactaccggg gcgagttctc ctttgcgcac
     3961 tgggacaagg ccggggattg atattacttc tacttaggag cctactatgt tatacgaacg
     4021 atacctgcgg gcctacgcag agcaattccg caagacagac cggaatatgt ggaactacga
     4081 ggatggctgt gtgctgatcg gcctgcgtgc gctgtaccag gcgaccggtg atgcgtttta
     4141 ctttgatgtg ctgaacagct ttatggagcg atacatcgag ccggacggta ccattcgccg
     4201 ctatgtggcc gaggaataca acctggacta catcccggcg ggctgcgcgc tgtataccct
     4261 gtatgacgag accggcgagt cgcgctaccg caaggccatt gaaacgctgg aagaacagct
     4321 gcgccaccag ccgcgcaccc cctgcggcag tttctggcac aagggcatct accccaacca
     4381 ggtctggctc gatggtctgt acatgggtct gccgttccat gcggcttacg ccctgcggtt
     4441 tggcgatgcg gccacgctgg atgacatcat gctgcaattc cgcaacgcac gcaagtatct
     4501 ctacgacgga aagacgggtc tatactacca cgcctggaac gagacaaagg atgtgttctg
     4561 ggctgacccc aagaccggcc tgtcccaaaa tttctggacg cgggccatcg gctggtattt
     4621 gatggcgatg gcggatatct acgccatgct gcccgacagc cgcgccgacc tgcaaaagga
     4681 gctggccgcc ctctggaaag aggccatcga cggcatgttg cgctggcagg agccggacag
     4741 cggcctgttc taccagctgc ctgccctgcc cgatgtgaag ggaaactacc tggagaccag
     4801 cggttccctg atggtggcgt acagcctgct gaagggcgcg cgcctaggcg tgctggccga
     4861 gccgaaatac cgtgcggcag gggagaagat tttggtcggc atcgagctgc ggcagttcac
     4921 cgtccacgac ggtaaggtcg agctgcacgg catctgcaag ggtgcgggcc tgggcccggc
     4981 gggcaacctg cgccgaaacg gcaccgtgga atactacctg tccgaggatg tggtccacga
     5041 cgagcagaag ggcgtcggcg tctgcatgat ggcctacgcc gagtatctgc aggccaaaca
     5101 ggccggtgcg ctgaccgacg acttccccaa agtggagatt ttcaacaagg ggtacgaccc
     5161 catcatgccc ggcgacccgg cgtttatcgc ctttgagaag aaccgcgtgg gcaaaacagg
     5221 gtgagctgta tgcagttttc catagcaaag ccggaggaag ttggcgtgtc cagtgcggcc
     5281 ctcagccaaa cgcttgatta tctggctcag cgtcgggtgg cgctgcacag cctgctggtc
     5341 atgcggcatg acgcgctgat ctgcgaaggg tacgcggcac cattcacaaa agatacgctg
     5401 catcgtatgt acagcgagac gaaaagcctt gtgtcgctgg ctgtcggcgt tatgtgcgcc
     5461 aaagggtatc tcgatttgga cgaccatatc gtggatcact ttgccgaaaa gctccccctg
     5521 ggcggcccta gccccgagct ggcacggctg accatccggg atatgctgcg catggcaacg
     5581 ccgtacaacg gcacgaccta caaacggcgg cccactaagg attgggtggg gtcgttcttc
     5641 accgcgcgga ccgaccacga gccgggcagt ttcttctgct atgataccag cagcagccat
     5701 acgttgtgcg ccctggtgga aaagcggatg ggccgccccc tactggatgt gctgcgccgt
     5761 gaggcactgg atgccatcgg cttttcagag caaagctatt gcatccggga tcccatgggc
     5821 ataagccagg gcgggtccgg cctgatggct accccccggg atatgctggc gctgctccgc
     5881 cttgttgcgc agggtgggca gtgggatggc caacagcttt taccccaaga ctatttgcag
     5941 gctgccacag cctggcaaat cgacaatgct gagaacggcg gcagcggcgg ctgggatttt
     6001 gcccaaggct acggctacca gttctggcgg ctgacccata acgcatggtc tatgtatggt
     6061 atgggcggcc agctggccat ctgcctgccg gatcaggata tgctccttgt gacgacggcc
     6121 gatacccagc ccctggcggg cggcgtgcag acgattctgg atgccttctg gaactgcctg
     6181 ctgcccggcg tggctgatgc tgcgctgccc gcaaaccctg cggcttatgc cgaactgacg
     6241 agaaagttat ccaccatgca gctgccgatt gtggaaaacc tggcggcccc cgacaccgag
     6301 ctttgctgtg cgacggtgca gatgggcctc aacgctcccg gcctgacggc cctgcagctg
     6361 caagaaaacg cactggtgct gcactacggc ggcaagacct gcacgctgcc tttccggacg
     6421 ggggcattgg tgcaaagcca tctgtgggac gaccccgcgc tgccctgtgt tatcgccgcc
     6481 ggctggcgtg ctccggactc cctccttttc cgggtgcatc tgctgggcga gcgactgggc
     6541 agcctgagtt tgcagctaaa gctgcgcccc ggcggtgcga ccctggctct gtgcagccat
     6601 gaggaacacc ccgaccccga tttcagcggc actgcggaag gtgtaaccgc agtgtgaacc
     6661 taagcgggcc gcctgctgcc atgcgcagcg ggcggctcgc gttattttta caggcacggc
     6721 ccttgcaaag tgcggcggaa tgtgctatgc tatttttgta caaaagggga gcttgcgtca
     6781 agcgggctga gagtgggctg tatcgccctg acccgtaacc tgatttggat aatgccaacg
     6841 tagggagata cgcgcacgac cactctttgt gagtttttat gcggatatgt ctttgcaggc
     6901 gtatccgcat tttacttgca aggagtgttt tttgtatgct gggtacctgt attgaaaatg
     6961 tacggaagaa cgtgccattg gtgcataaca tcacgaatta tgtcaccgtc aatgatgtgg
     7021 cgaacatcct gctggcctgc ggcggcagcc ccatcatgtc cgacgagccg gaggatgtcg
     7081 aggatatcac caccatctgc ggcgggctga acatcaacat cggcacgctg aaccagcgca
     7141 gcatcgcggg gatgttccgc gccggggcca gggccaacgc cctgggccat gtcgtgctgc
     7201 tggatcccgt cggcgcgggc gcgtcggcac tgcgcaccaa cacggctgtg gagctgatgg
     7261 aaaaggtcaa gttcaccgtt atccgcggga atatttccga gatcaagacg ctggcgctgg
     7321 gcagcggcac gaccaagggc gtggacgccg acgtggccga cgctgtgacc gatgcaaacc
     7381 tggacaatgc cgttgcattt gtcaaggatt ttgccgccaa gagcggggcc atcgttgctg
     7441 tgaccggggc aatcgacctg gtcagcgacg gcaagacctg ctatgtcatc cgcaatggcc
     7501 gccccgagat gggcaagatc accggcacgg gctgccagct ctccggcatg atgacggctt
     7561 tcgtcgtcgc caaccccgac aaccggcttg aggccgccgc cgctgccgtc tgcgccatgg
     7621 gcctggcggg cgagatcggc tggagccgta tgcaggacgg cgacggcaac tccacctacc
     7681 gcaaccgcat tatcgacgcc atctacaaca tggacggcgc aacgctggac gggggcgcaa
     7741 agtatgaact gcgataaaaa ggatctgctg ctctacgctg tcaccgaccg ccattggctg
     7801 gacggccgca ggctcatcga cgttgtgcgc gagagcctgg acggcggcgt gacgatggtg
     7861 cagctgcggg agaaaacgct ggaagaggga aaattcctcg aagaagcgaa ggaattgcag
     7921 gcgctttgcc gcgaacgcgg cgtgccgttc cttgtgaatg acaacgtgga aattgcccgc
     7981 gagatgaacg ccgacggcgt ccacgtcggc cagagcgata tggaggcact ggacgtccgc
     8041 gccattctcg gccccgacaa gatcctcggc gtgtctgcgc agactgttga acaggccgtc
     8101 ctggccgaaa agcacggcgc ggactacctt ggcgtcggcg cggtgttccc gaccggctcc
     8161 aaggacgatg ccgacgacgt gagctacgac acgctgaaag ccatctgcca agctgtctcc
     8221 atcccggtcg ttgccatcgg cggtattaca cagcagaacg ttgaaaagct ggcgggcagc
     8281 ggcatctgcg gcgtggcggt catcagcgcc atctacgcgg ccaaaaacat cgggcaggca
     8341 tcgaaggatc tgaaagccgc cacggagaaa atgctgcatg actaagggcg caatttttga
     8401 tttggacggc gtgctgctcg actccatggg catctggaac gacctgggtg cgcggtatct
     8461 gcgcagccgg ggcctgatgc ccgagccggg cctcaacgag attttgttct cgatgagcat
     8521 ggagcagggc gcggaatacc tgcacaccca ctacccgctg gcccagtccg ctgccgaaat
     8581 tgaagcgggc attgagacga tgctgcgcga tttttactac aacgaggtcc ccgccaaacc
     8641 cggcgcggcg gcgctgctgg ccgcgctggc ggcaaagggc atcccgatgg cggcggcgac
     8701 ctccagcccg cgcgggcatg tgacccacgc tttgcagcgg ctggggctgc tcggctattt
     8761 tgcgcagatc ttcaccaccg gtgaggtcgg cgtcagcaag cacgagccga cgatttacct
     8821 gctggccgcg caggccctgg gcagcgcacc gggggagacg actgtgtttg aggacagcct
     8881 ctacgcgctg aaaaccgcca aagccgcagg gttttacacg gtcggtgtct atgacgccga
     8941 cggcgaaagc gaccaggccg ggctgaaagc tgcggcagat ttgtatgtga aggagctggg
     9001 ggaggctgcc gggaggtggt aaagcctctc ggcctcgtaa actcgccctg ccacctaaca
     9061 aaaacgcggt acaccggggg caccaccttg tatcgcgcaa taaaacattg ctgtatatat
     9121 aggaaggaag tatcattatg aaaacagcat tgaccattgc aggcagtgat tcctgcggag
     9181 gcgccggtat ccaggcagac atcaagacca tgaccgccaa cggcgtttac gccatgagtg
     9241 cgatcacggc cctgaccgcc cagaacacta ccggcgtgac ggacatcctt gaatcgaccc
     9301 cgcattttct ggcggaacag ctcgacgcgg tgttcaccga tatcaccccc gacgccgtca
     9361 agatcgggat ggtctcctcg gcggagctca tcgagacgat cgccgccaag ctgaaacagt
     9421 acggcgcaaa gcatatcgtc gtggaccccg tcatggtcgc aacgtccggt tccaaactcc
     9481 tgcgggacga cgccgtcagc gccctgtgcg caaacctgct gccgatggct gaggtcctga
     9541 cgcccaacat ccccgaagcc gagatcctgg ccgatatgcc gatcaaaaac gccgccgaca
     9601 tggaaaaggc cgcgcagacc atcagcgaaa aatacggctg tgcggtgctc tgcaagggcg
     9661 gccaccagat caacgatgcc gacgacctgc tgtggcgcgg cggcgcgggc aaatggttcc
     9721 gcggcaagcg catcgataac cccaacaccc acggcaccgg ctgcacgttg tccagcgcca
     9781 ttgcgtccaa cctggcaaaa ggctatgacc tcgacgcttc ggtcgagcgc gcaaaggcct
     9841 atatctccgg cgcactggcc gccatgctgg acctgggccg cggctccggc ccgatggacc
     9901 atatgtttgc gctgaaaggg gagttcaccg aatgaaaaag acttccactt tccaaaacgg
     9961 cctgatttgg ttcggcgcgg gcgtctcgct ggcagaaatc ctcacaggta cgtcctttgc
    10021 accgctgggc atggcaaagg gctttgcggc tatcctcatt ggccacatca tcggctgtgc
    10081 catgatgctg ctggcgggcc tcatcggcgg ccgcaccggc cgcagcgcca tggagaccgt
    10141 caagatgagc ttcggccaga agggcggcct gctgttcgcg ttcctgaacg tcctgcagct
    10201 cgtcggctgg acggccatca tgatttacga cggcgcactg gcggtcggcg gcatttttgc
    10261 gatcggccgc tgggtgtggt gcctggtcat cggggcgctg atcgtgctgt ggatcgctgt
    10321 gggcatcacc gatttgggct ggatcaacaa aattacgatg gccgctttgt ttattttgac
    10381 gttggttttg tgcaaggtca tcttcttctc cggcaacgcc atggtcggcg tcggcggcga
    10441 gagcctgacc tttggcgcgg cggtggagct ggccgtggcg atgccgctgt cctggctgcc
    10501 gcttatcagc gactacaccc gcgacgccga aaagcccacc caggccacct gggtcagcgt
    10561 gctggtctac ggcgcggtgt cctgctggat gtacgtcatc ggcatgggtg cggcgatttt
    10621 caccggcgag tatgacattg ccgtcatcat ggtcaaggcg gggctgggca ttgccgcgct
    10681 ggtcattctg gtgttttcca ccgttaccac gacatttttg gatgcgtgga gcgcgggcat
    10741 ttccgccgag agcctgtccg ccaaactgga cggcaaaaaa acggccatcg tcgtgacggt
    10801 catcggcacg ctgggggcta ttctgttccc gatggacgat atcacgggct ttttgtacct
    10861 gatcggctcg gtctttgcgc cgatgatcgc ggtgcagatc gccgaccact ttatcttaaa
    10921 gcgcgaccgc tttgccgcgg ccgccgacgc gcgcaatctg gtcatctggc tggtgggctt
    10981 catcgcctac cgcctgctga tgggcgttga caccccggta ggctacaccc tgcccgatat
    11041 gctgctgacc gtggcaattt gcgttattgc cgagaaggtc aagccgacga aggcgtaaat
    11101 ccatagttta tagtgcgtgg gggttctgtc catcagaacg cccacgcttt tttgcgtatc
    11161 ccaggcaaag cgaaggccgg agggggcttt gccgccgtgc ctgacttgcc cgctttcccc
    11221 gccgatagca gccacgaaac agattttgcg caatatacgc gcaacaaatg cgcaataaat
    11281 ccgacaaaac ctgccaagtt tgcctaactg cgcaactgat tcgacacgag ttgtacaaga
    11341 ccaactgacg aagtgtacaa aaatatgact gaatcttttt gcaaaacgcc gaaaatgagc
    11401 aaatcaagca ggatatattg cgatttcgca acaagagtgc tattctttta tataagaaat
    11461 gtaaaaacca ggtaggcagc ggcagaaaaa ggctgctgtg tatttttaac gaggttgcgc
    11521 accagcggtt gcgcaacggg aaaggggccg gcccctttcc tattccacag cgggctgtgg
    11581 aataataagc tctgtcaggt aagggagggc aggctgatga caaagaaatt ttctgtactg
    11641 cagcgcgcag cggcattgct ggccgctctg tgcctgctgg cgggtttggc tctgcccgtc
    11701 tgcgcggagg gcaccgacga cgccctttct gcgcagaatg tggaaacgat acagcaaggg
    11761 aacgacccca ccgtcgaaac cctgccgggt gagcagaccg acgaggacaa gaccgccccg
    11821 gacggcgcgg ctgccgcgac cccgaccccc acgccggtgg caacgcccac cgcgaccccg
    11881 gcccccacgc cgacggcagc gcccgccgcg accccgaccc ccgcgccgac ggcaacgtcc
    11941 accgcgaccc cgacccccac gccgacggcg atgcccactg cgaccccgac ggcgacaccc
    12001 accgcgaaac cgaccccgaa accgacgccc accgtgacgc cgataccgga tgacgaggag
    12061 aacagcgaaa gcgaagaaaa atttgtaacc ttcgcgaaag tcaatgcagt tgcggagggc
    12121 ggcaaaccgg tagacggcac gacgtacacc ttctactttg ccccgcctgc cgactggggc
    12181 gaagaggttg aattcagagg caagcgcggc gggggtaatc aagacaatga tactgagata
    12241 atccgcccaa tgagccgagt cccaggctat acggccagta ataaacgccc aatttatgtc
    12301 gtgagtctgc gataccaaga gggcaacaat ggggcggaat gtacacatgg cggttatgta
    12361 tatataaagt ttatatcaaa atcaggtgag cataccattc aaatgaatgg gcctaacaac
    12421 tggctgtcta ttgatgccat gggggaccat atctttgacg cgggcggcct ggaggataaa
    12481 acatacacca catggaaccc caactgcctg aaaccctacg ccgaggtcgc ccagcagatt
    12541 ccctacacca accagaaaat ctcgtttcaa aacaaaacgg agaacgaact caccaatatc
    12601 aaagtgacgt tctacgatga taaatatgtg gcattcggca agccgcagac cattgaatcg
    12661 cttgaagcag gcaaagtctc tgagggaatc gagattccgg atgattcttg ccgctatgtc
    12721 ggcttcaccg atgcaagcgg caaacccatc ggcaaaacgt actataactt ctacaacgat
    12781 gacgttgctt cggaagaggt aggtacattc cagtataacg ccgaaacgaa ttactgcttt
    12841 atctacaagg gggcgtctga cgtccagtgg ggcgagccca acacaacacg catttacttt
    12901 gacgcgacgt tctcggatat gtcgtatagt ggagattccg gaacaacggc agacggaatg
    12961 ccgggaaacg acggaaagct ttactatatc ctgaccgggg aggacaaaac ggagcagaca
    13021 ggtgagatga cccggctgga ggatcgttcc gagtcacgac acctttggta tgtaaatgcc
    13081 ccggacggct acacgaaagt acagttctcc gatacaaaaa ccccgccggc gggcagcgat
    13141 atcaatggcc gtgcaacgga cacgctgaca tgggggaacc tggaagagcc gtgcttctat
    13201 gcggacaccg gcgatgacat tacctacgtt ggtgggtatc gtggaggcta ttggggtgaa
    13261 aaatctgata tccgcgatgc ggaagcgggc aagaagaagg tcgaccagaa cagggaaccg
    13321 gtcgttaatc ttgaaaagaa ggaatttacc cagcaagcta acacaaagta tatcagtaca
    13381 acgctgtatg actactacac ggattacgag ctgaacggca ataaccgagc aaaaaataac
    13441 tgcacagaat atagcaacca gcgcagttat gtcacgtttg aacagtttga ccgtgcccta
    13501 agtgattatt acagcgagta tgccactgaa aagaatacgc caatccagta cccgctttat
    13561 accggtcatt tccagccaag caatctgggt gataataata ggtttgcaaa cattgcaaat
    13621 aacctgggac tgtacggctg gggcttgaaa ctgggggagc gtatcattgt gcctcccaaa
    13681 tacaggaaga ttctgcctcc tgtgggctat tattg
//
LOCUS       DBMDSQ010000027        13135 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_1440_length_13135_cov_2.236305, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000027 DBMDSQ010000000
VERSION     DBMDSQ010000027.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 13135)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 13135)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13135
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1440_length_13135_cov_2.236305"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..309)
                     /locus_tag="ACZPAX_01945"
     CDS             complement(<1..309)
                     /locus_tag="ACZPAX_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1112693.1"
                     /translation="MTKNKQQKNALLTILCVLALAAFGAAMVWLHYQQLCSPGGGDDP
                     YTSDLGQHLAFAQRGMVYSTVSLLIGPAYNLAGRMGIAVLLAVFHLAAVAVFACGLRA
                     A"
     gene            complement(306..1529)
                     /locus_tag="ACZPAX_01950"
     CDS             complement(306..1529)
                     /locus_tag="ACZPAX_01950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1112694.1"
                     /translation="MAQNKKQRLLWGLGLLGTAVFTLLMGWLHYQQLLSTGYYYSDIP
                     AHMAGHAMGNTGFSLVTVLLQGVFAIGGNLGTAVFLAVVNLLTLLIFAWAIRKAVPQV
                     CPGAALLWSLAANLCQAVWMPNGGYWYFGAITGTIYHNTTYIMLQPLALIAFFLFLSL
                     AEHHGQLQWRSWAALTVLLTVATAIKPSYLFAFAPALLVALVVDLIRTHGKALQWEVW
                     LGCTVLPGILLCIIQAKVLFTGGDDSGMALIFTTDFDPEKVLWGVFNYSAKHGLVRSL
                     VFVGAVVLLLFRQWRGAYAYKFSLLLFAVSLAEGICLTETGSRMYDGNLWWGAFICYD
                     ILLLESLIQLLRRLHKKPRTPVETGTSVLCCGALAWHIASGIVFLGLMLAGISYAVMI
                     GTESYLLQEMWGIVL"
     gene            complement(1707..3461)
                     /locus_tag="ACZPAX_01955"
     CDS             complement(1707..3461)
                     /locus_tag="ACZPAX_01955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1112695.1"
                     /translation="MKLQKLTEKRWFWPLVCAVSVVFGWWYLSIVTCAPLGGDDELIN
                     LQNYYYITHTSFGQSVLDYIGDLWTQFSLQQGRFRPFSSPPVRGLTSWFLGDLVGYRL
                     YILAWTYADILLTGWLVGKASHNKKLGIACICLLPMMFSVWQDSTGNSLYSYGALVQS
                     TLLPALVAGLAVLRLQDTGHKRWAVLAGYCAFQCCATFEIGFTYIVPIFGLAWLYTDK
                     ARDALRLSIPVFVGECVTLAFNLGARLVNTLQEMGVLAGDAQPIGGVSPNFDIPAVLK
                     TWAMQMSAGFPLNALFAGKVRPGTIYPVDILCGVMVAGAAVAALAALHELPNRKQNLL
                     LFLTGLAMLSAPSLLIGLSPKYQQAGQIDWRHGYIPQTVESYGVGLMALALLVLLLRW
                     ARSKTWWPKSRAVLYCLLAVGMAGTVVWQRAATRSAYAEGGRAYTVFGETVAAGAADA
                     AGTEMPVVTDYMIWGGNEVAENAFFLRYGEQDADAHALQVWRTEDHADETAYRLGFTL
                     GQTNRYDIGWCGLGSGNALDELTDVVVCLPAGTAQQATLCYTTADGEEVRTEVYAEKT
                     ATLVQLEGTVLAESIRLE"
     gene            complement(3465..5249)
                     /locus_tag="ACZPAX_01960"
     CDS             complement(3465..5249)
                     /locus_tag="ACZPAX_01960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1112696.1"
                     /translation="MPKSKQNLAREILTVALLLAAVAASEYWFFQLWRLDWSVPMLYG
                     GDGIYWVGQVQRSYGELSGSLGWPFYEAAGKYDPNYDLIYDIFVWFVGLFTKDTGTVF
                     NLYVLVIPFANALAAYAVFRMVGMRRWLSFAFGITFGMTPYVQQRMAGHMMLAACEFV
                     PFSVLLCLWCAEDEGFNRPGKGFFKNKRNWLALAMAWGIANNGAAYYPYFTCFFLCVT
                     ALCLILRGHKWSAGVPCLVTIAEIVAWMIPDFFPMVLGILSGQGSTLTNGVYRSPVGA
                     DIYSLRISSLLLSPNGFGLQKLANWMGRYFHILATDEGPMYNENGYGYLGVVGIFGFL
                     ALLLMLLRNWDWKAGRTEKPQLGDRLWLLSRLNVMALLLASIAGFGSIIGIFVRFIRG
                     YNRISPYIAFFALLAVGLTAEMRLTRRAGKDRKKLILVMVVLLAYGYWEQQGLFSPKY
                     EEVQMSWHQDAAFMAEVEDAAGEGAMLFQLPYMKNFENGPLNNMWDYTLLRGPLHSKT
                     LKFSYGAGYGTENDAWYQATSELEPDAMVAELRTQGMAGIYLDLDGYTEDEQQPTLAA
                     LIQAAGCAEDDVIISESGKLCYIPLGQE"
     gene            complement(5300..6040)
                     /locus_tag="ACZPAX_01965"
     CDS             complement(5300..6040)
                     /locus_tag="ACZPAX_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1112697.1"
                     /translation="MPIIQVEDVSMRFNLAQEKTETLKEYTVKLLKHQLFFNEFYALQ
                     DVSFSIEPGESVALIGRNGSGKSTMLKLIAGVMYPTRGTVAVNGEIAPLIELGAGFDL
                     DLTARENVFLNGAVLGHDRTYMEEHFKNIIDFAELWDFVDVPVKNYSSGMIARLGFSI
                     ATEVKADILACDEILSVGDFMFQQKCHQRMEQMLSGGTTLLFVSHDINQVKQLCKRAI
                     WIDHGHIRGDGPAEEVCNAYVEAMQRGE"
     gene            complement(6053..6850)
                     /locus_tag="ACZPAX_01970"
     CDS             complement(6053..6850)
                     /locus_tag="ACZPAX_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1112698.1"
                     /translation="MQKSTGQFHNSWRNFSKYRPLIRELVTRDLKVKYRRSVLGYVWS
                     ILNPLLMMLLQTLVFSYMFRFDIPNFPLYLICGNTLFNFFNESTNMGMGSVLGNSSLI
                     KKVYVPKFIFPISRVVSSFVNLLFSLAAIILVMLITRSPFYWTILLVWAPLLLLLAFC
                     CGMAVLLSALAVYFRDLQYLYGIVTMAWMYATPLFYPISALPPFLIPIIKLNPLYHYI
                     NCMRCLVMYGTVPGPNTWIACIGSAAVMLAVGLAAFRKLQRNFILYL"
     gene            complement(6916..8181)
                     /locus_tag="ACZPAX_01975"
     CDS             complement(6916..8181)
                     /locus_tag="ACZPAX_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005930493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HIR1112699.1"
                     /translation="MPNENEHPRYTDPDHPYSGSGLRYQNNYNRNEYHAGDGPFQFPW
                     WAIVIGFVVWWPLGFIFIGLNAAMKNGKLGSFEQKARTRTQSYRSGPSVQEGQFRPIY
                     AQQDKESAGKTAAAPRDAEKKRKGMALANGLTAAGVLLLLTALAALPGGTYWLPDALT
                     GDLEYIRWFLADMMPVFVSATAGAGCLFGAHHIRTGRRLRKKIDNIVGDAQYMYIKDI
                     ADAIPCSYEKCCKHLENCIDDGVFGPEAYLDMRRKCLVVTGKPPEPTPKKAKKPKVEK
                     ADPMPEKDQYQKILDELRRVNDAIPDEEMTDKISRLEAVSAKIFEQAKSDPDKLPQMR
                     KFMDYYLPTSLKLLNTYAELDNQGVEGENISESKRRIELTMDTLVKAFENQLDRLFAS
                     DALDVSADIDVMQNMLRADGLTDDAPFKM"
     gene            complement(8193..9350)
                     /locus_tag="ACZPAX_01980"
     CDS             complement(8193..9350)
                     /locus_tag="ACZPAX_01980"
                     /inference="COORDINATES: protein motif:HMM:NF012863.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HIR1112700.1"
                     /translation="MPTPQNREPGRRPRNPDTERYTMQGDWAVPNQHPTAPPQRRAPT
                     QEQRRAAARRRREIRRRRRRLAVLGTVGGVLVLSGIITVLLPKSMKGETEAAPLPTAD
                     PAGTRLVAPLPYAGAGDSGSQNAQTLNWGTVGPQRQTADTGYTYTVLPAKPAALPEFG
                     RVDTSWFADAAFLGDSLTAGFCANEYNIDVGGALICGYEGISPNTVVNRTTVTSPDRG
                     EEVPLDVLAAAQPAKLYILIGTNALVQPGNDDSFLAYYGKMLDDLRTALPDTTFYVQS
                     VLTATQEKVSDSLPGLAPDRLAAINAAIQQLCAERGCYYLDLNAEFADDAGYLQTDFS
                     QPDGVHLTVSGYSKWVSYLCTHVPYSKNNPYQAGSTYYLSDDMKNLLNDIP"
     gene            9577..11238
                     /locus_tag="ACZPAX_01985"
     CDS             9577..11238
                     /locus_tag="ACZPAX_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HIR1112701.1"
                     /translation="MADEKFSNFLTDIIDADLAEGKVDVVHTRFPPEPNGYLHIGSAK
                     AIFINYTIAKHYGGLFNLRFDDTNPAREGDEYVQSILQDLKWLGAEPNGGIFYGSDYF
                     ERCYEYAEQLIKEGKAYVDDLTREQMQEYRGNDAGKPSRPSPYRDRTPEENLDLFRRM
                     RAGEFADGEKTLRAKIDLASPNMNMRDPTIYRIKHVEHHRQGDKWCIYPMYDFAHPIQ
                     DAIEGITFSLCSLEFENHRPLYEWVITNLFGKEFPKQREFARLNVTNTVMSKRYLREL
                     VEKNIVDGWDDPRMPTLSGLRRRGYTPTSIFKFVKDAGISKADNLVDMRQLEAVLRAE
                     LELNAQRRVAVLDPVKLIIDNYPADQCEYFDLPNNPNRDANDSTTRKVAFTKELWIEN
                     SDFFEVPPPKFKRLTLGSEVRLMGAYLVRCTGVDKDENGKVVAIHAEADLETRNGNPA
                     DGRKVRGTIHWVSCAHCLDAEVELYDKLFTESNMNSIPDDADFKDYLNPDSVKVLQGA
                     KLEESLKDAKPGDRFQFVRNGYFTPDSKHEGVYNRIVTLKDSFKL"
     gene            complement(11288..12574)
                     /locus_tag="ACZPAX_01990"
     CDS             complement(11288..12574)
                     /locus_tag="ACZPAX_01990"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1112702.1"
                     /translation="MFQQLYNTADTLIVGNFVGTNALAAVGATSSFVNLLVGLFVGLC
                     SGAGVVIAQSWGAHDADAVDRQVHTALVLSVVIGALLTVLGLLTAEPMMHLMGTPAEI
                     LDDAALYLRIYFLGMIPQMLYNMGTNILRAIGDSKRPLYFLMAASLVNIALDVVFIAV
                     FGWGVAGAAWATVLSQVASAALTLRCIVGSQGMPWHVRPEKLRLHGDVFAAICTIGVP
                     AAAQSAMYNISNIFIQSSMNSFGTSTIAAWGVYGKIDFVFWMTINSLGIAITTFAGQN
                     FGARQYDRVRRGVRVCLAMAAGITLLISVTFYNLAEPLFRLFSQDDAVVAVGVGMMHV
                     LTPVYITYICIEVLAGALRGCGDVRIPTLITVFFVCGLRMLWLSVAVPIRHEVATVEL
                     SYPITWTLASALFIVYYLKGGWMQRCAEKWEKKQKA"
     gene            12737..>13135
                     /locus_tag="ACZPAX_01995"
     CDS             12737..>13135
                     /locus_tag="ACZPAX_01995"
                     /inference="COORDINATES: protein motif:HMM:NF024702.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat protein"
                     /protein_id="HIR1112703.1"
                     /translation="MTVFWEPCAGGVRVLRLLGDTPCPAVPGVIGGLPVVEIGPYCFA
                     EKPVRPGAQCTGTDTHEITGNFVEEVTLPDTVRVLHSAAFYNCRKLRRVTLGAGVASL
                     GSDLFTNCRQLRVFTLRAAADAPTGLKKLLG"
BASE COUNT         2733 a         4023 c         3944 g         2435 t
ORIGIN      
        1 cgcggcccgc aggccgcagg caaacaccgc caccgccgcc aagtggaaca ccgccaacag
       61 cacggcgatc cccatgcgcc cagccagatt gtacgccggg ccgatgagca gcgacacggt
      121 cgagtagacc atgccccgct gggcaaacgc cagatgctgc cctaaatccg atgtgtaggg
      181 gtcatccccg ccgccgggcg aacaaagctg ctggtagtgc agccacacca tggctgcgcc
      241 gaacgctgcc agcgccagca cgcataaaat cgtcagcagg gcgtttttct gttgcttgtt
      301 cttcgtcaca gtacaatgcc ccacatttct tgcagcaggt agctttcggt gccgatcatg
      361 accgcatagg agataccggc cagcatcagc cccaggaaca caatgccgct ggcaatatgc
      421 caggccagcg ccccgcagca caaaaccgac gtccctgtct ccaccggggt gcgcggcttt
      481 ttgtgcaggc ggcgcagcag ctgaatcagc gattccagca gcaggatgtc atagcagatg
      541 aacgcgcccc accacaggtt gccgtcgtac atacggctgc cggtctcggt caggcagatg
      601 ccctcggcca gcgagacggc aaacagaagc aggctgaact tataggcgta agcgccgcgc
      661 cactgccgga acagcagcaa aaccaccgca cccacgaaca ccagtgagcg caccaggccg
      721 tgcttggcgc tgtaattaaa gacaccccac agcacttttt cggggtcgaa gtcggtggtg
      781 aagatcagtg ccatgccgga atcatccccg ccggtgaaca gcaccttagc ctgaatgatg
      841 cagagcagaa tgcccggcag caccgtacag ccgagccaga cttcccattg gagggcctta
      901 ccgtgggtgc ggatcaggtc aacgaccagc gccacaagca gggctggggc aaaggcgaaa
      961 aggtagctgg gcttgatagc cgttgccacc gtaagcagca ccgtcaacgc ggcccaactg
     1021 cgccattgca gctgaccatg gtgctctgcc agcgagagga agaggaaaaa cgcgataagc
     1081 gccaacggtt gcagcataat gtaggtagtg ttgtggtaga tcgtgccggt gatggcacca
     1141 aagtaccagt agccgccgtt tggcatccag accgcctgac agaggttcgc agccagcgac
     1201 cagagcagcg ctgcgccagg gcagacctgc ggcacagcct tgcggatggc ccaggcaaat
     1261 atcagcagcg tcagcaggtt gacgacagcc aaaaacaccg ccgtgcccag gttgccgccg
     1321 atggcgaaca cgccctgcag caggaccgtg accagcgaaa agccggtatt gcccatggca
     1381 tggcccgcca tgtgggccgg gatatcagag taatagtacc cggtggagag cagctgttgg
     1441 tagtgcagcc agcccatcag cagggtaaag accgctgtgc ccaacagccc caggccccac
     1501 agcagccgct gtttcttgtt ttgtgccaaa gcggcacctc cttggtgtgt aatggcttcc
     1561 cccatggggg aagctgtcag cggcttgacc gctgactgat gaggggcggc cttgccgatg
     1621 cagaccgttc acgggttgct gtggcacacg cgcccctcat ccggcgctgc ggcgccacct
     1681 tcccccaaaa ggggaaggca aagaaattac tccagccgaa tactctccgc cagcactgtg
     1741 ccttccaact gcaccagggt ggcggttttt tcggcgtata cctcggttcg tacttcttcg
     1801 ccgtccgctg tggtgtagca aagcgtggcc tgctgcgccg tacctgcggg caggcatacg
     1861 accacatccg tcagctcatc cagcgcattg ccgctgccaa ggccgcacca gccgatgtca
     1921 tagcggttgg tctggcccag ggtaaagccc aggcggtagg cagtctcatc ggcgtggtcc
     1981 tcggtacgcc agacctgcaa ggcgtgggcg tcggcgtcct gctctccata gcgcaggaag
     2041 aacgcgttct cggcgacctc gttgccgccc caaatcatgt aatccgtcac aacgggcatc
     2101 tcggtccctg cggcgtcagc cgcaccggcg gctaccgtct cgccaaagac cgtgtaggcg
     2161 cggccaccct cggcgtaggc tgagcgggtc gcggcgcgct gccagaccac cgtgcccgcc
     2221 atgccgacag ccagcaggca gtagagcacc gcgcggctct tgggccacca ggttttgctc
     2281 cgtgcccagc gcagcagcag caccagcagc gccagggcca tcaggccgac gccgtagctt
     2341 tccaccgtct gcgggatgta gccgtgccgc caatcgatct gccccgcctg ctgatattta
     2401 ggcgaaagcc cgatgagcag cgacggtgcg gacagcatgg caaggccggt caagaacagc
     2461 agcaggtttt gcttgcggtt tggcagctca tgcagggcag ccagcgccgc caccgcagcc
     2521 ccggccacca tgacgccgca gaggatatcc accgggtaga tcgtgccggg gcggaccttg
     2581 cccgcgaaca gtgcattgag cgggaagccc gccgacatct gcatggccca ggtcttgagc
     2641 accgccggga tgtcaaagtt cggcgataca ccgccgattg gctgggcatc cccggccaga
     2701 acgcccatct cctgcaaggt gttgacgagc cgcgccccca ggttgaaggc cagcgtcacg
     2761 cactcgccca caaacaccgg aatgctcagc cgcagggcgt cgcgggcctt gtcggtgtag
     2821 agccaggcca ggccgaaaat cggcacaata taggtaaaac caatttcaaa cgtggcacag
     2881 cactggaacg cgcagtaccc cgccaacacg gcccaccgct tgtgcccggt atcctgcaaa
     2941 cgcagcacag ccagccctgc caccagtgcg ggcagcagcg tactttgcac cagcgcaccg
     3001 taggaataca ggctgttgcc ggtggagtcc tgccagacac tgaacatcat gggcagcagg
     3061 cagatacagg cgatgcccag ctttttgttg tggctggcct tgcccacgag ccagccggtc
     3121 agcaggatat ccgcgtaggt ccaggccaga atgtagagcc gatagcccac cagatcaccc
     3181 aaaaaccagc tcgtcagacc gcgcaccggc ggcgaggaga agggccggaa ccgcccctgc
     3241 tgcaagctga actgggtcca caggtcgccg atataatcca gcacgctctg cccaaaggat
     3301 gtgtgggtaa tataatagta gttttgcagg ttgatgagtt catcgtcgcc gcccaggggc
     3361 gcacaggtca cgatgctgag ataccaccag ccgaacacga ccgacacagc gcagaccagc
     3421 ggccagaacc atcgtttttc cgtcagtttt tgcagtttca ttgctcactc ctgccccagc
     3481 gggatatagc agagcttgcc gctctcgctg atgatgacgt cgtcctcggc acagcccgcc
     3541 gcctgaatca gcgctgccag ggtgggctgc tgctcgtcct cggtgtagcc gtccagatcc
     3601 aggtagatgc ccgccatgcc ctgggtgcgc agctcggcca ccatggcgtc cggctccaac
     3661 tcgcttgtgg cctgatacca ggcgtcattc tcggtgccgt agcccgcgcc gtagctgaat
     3721 ttcagcgtct tgctgtgcag cgggccgcgc agcagcgtgt agtcccacat attgttcagc
     3781 gggccgttct caaagttttt catgtacggc agctggaaga gcatagcccc ctccccggcc
     3841 gcgtcctcga cctcggccat aaaggccgcg tcctggtgcc agctcatttg cacttcctca
     3901 tatttcgggc tgaaaaggcc ctgctgctcc cagtagccgt aggccagcag caccaccata
     3961 acgaggatga gctttttgcg gtcttttccg gcgcgccggg tcagccgcat ttccgccgtc
     4021 aagccgacgg ccagcagtgc gaagaacgca atgtaagggc tgatgcggtt gtacccgcgg
     4081 atgaaccgca caaagatacc gatgatgctg ccaaaaccgg caatgctggc taacagcagc
     4141 gccataacgt tcagacggct cagcagccag aggcggtcgc ccagctgcgg cttctcggtg
     4201 cggccggcct tccagtccca gttgcgcagc agcatgagca gcagcgccaa aaatccgaaa
     4261 atgcccacga cgccgagata gccgtagccg ttctcgttgt acatcgggcc ttcatcggta
     4321 gccaggatgt ggaagtagcg gcccatccag ttggcgagct tttgcaggcc gaaaccgttg
     4381 ggcgacaaca gcaggctgct gatgcgcagg ctgtaaatgt ccgcgccgac cgggctgcgg
     4441 tatacgccgt tggtcagggt gctgccctgg ccgctgagga tgcccagcac catggggaag
     4501 aaatcgggta tcatccaggc gacgatctcc gcgatcgtga ccaggcaggg cacacccgcg
     4561 ctccacttgt ggccgcgcaa aatcaggcag agcgccgtga cgcagaggaa aaagcaggtg
     4621 aaatagggat aataggccgc gccgttgttg gcgatgcccc aggccatagc cagggccagc
     4681 cagttgcgct tattcttgaa aaagcccttg cccgggcggt tgaagccttc atcctcggca
     4741 caccagaggc agagcagcac cgagaagggt acgaactcgc aggcggccag catcatatgc
     4801 ccggccatcc gctgctgcac gtagggcgtc ataccgaagg tgatgccaaa ggcaaagctg
     4861 agccagcggc gcatgcccac catgcggaac acggcgtagg ccgccagcgc gttggcaaag
     4921 gggatgacca gcacatacag gttgaacacg gtgccggtat ctttcgtaaa caggccaacg
     4981 aaccagacga agatatcata aatcaggtcg tagttcgggt catatttgcc cgcggcctca
     5041 tagaacggcc agcccaggga gccggacaac tcgccgtagc tgcgctgcac ctgacccacc
     5101 cagtagatgc cgtcgccgcc gtagagcatg ggcaccgacc aatcgagccg ccacagctgg
     5161 aaaaaccagt attcgctggc cgccaccgcc gccagcagca gcgcgacggt cagaatttcc
     5221 ctggccaggt tttgttttga ttttggcatg gggtctcctt taaaagttct gccttccccc
     5281 tggggggaaa cctttggggt cattcccctc tctgcatagc ctccacatag gcgttgcaga
     5341 cttcctccgc cgggccgtcg ccgcggatgt ggccatggtc tatccagatg gcgcgtttgc
     5401 agagctgctt gacctggttg atatcatggc tgacaaaaag cagcgttgta ccgccggata
     5461 acatctgttc catccgctgg tggcactttt gctggaacat aaagtcaccc accgacagaa
     5521 tctcatcgca ggccaagata tccgccttga cctcggtcgc aatggagaat cccagccgcg
     5581 cgatcatgcc gctggaatag tttttcaccg gcacatcgac aaaatcccac agttcagcaa
     5641 agtcgatgat atttttgaag tgctcttcca tataagtgcg gtcgtggccc agcaccgcac
     5701 cgttcaaaaa cacgttttcc cgcgcagtca aatcgaggtc aaagcccgcg cccagctcga
     5761 tcagcggcgc aatttcgccg ttgacggcga cggtacccct ggttgggtac atgacgcccg
     5821 caataagttt gagcatcgtg cttttgccgc tgccgttgcg gccaatcagc gctacggatt
     5881 cgccgggttc gatgctgaac gagacgtctt gcagcgcata aaattcattg aaaaaaagct
     5941 gatgtttcag cagcttgacg gtatattcct tgagggtctc ggttttctcc tgcgccaggt
     6001 taaagcgcat cgagacatcc tccacctgaa tgatgggcat gggggaaact ccttacagat
     6061 acagaataaa gttgcgctgc aatttgcgga aagccgccag gcctacagcc agcatgacgg
     6121 ctgcgctgcc gatgcaggca atccaggtgt tggggccggg cacggtgccg tacatgacca
     6181 ggcaccgcat acagttgatg tagtggtaca gcgggttcag cttaatgatg gggatcaaaa
     6241 acggcggcag cgccgagatc gggtaaaaca gcggcgtcgc gtacatccag gccatcgtca
     6301 cgatgccgta gagatactgc aaatcgcgga agtagacagc cagcgccgag agcagtacgg
     6361 ccatgccgca gcagaacgcc agcagcagca aaagcggcgc ccagacgagc agaatcgtcc
     6421 agtaaaacgg cgaccgggtg atgagcatga ccaggataat ggccgccagg ctgaacagca
     6481 ggttgacaaa gctggacacc acgcggctga tggggaaaat aaacttgggc acgtacactt
     6541 ttttgataag ggacgaattt cccagcacgc tgcccatacc catgttcgtg ctctcgttaa
     6601 aaaagttgaa cagagtgttg ccgcagatga ggtaaagcgg gaagttcggg atatcgaacc
     6661 ggaacatata ggagaaaacg agggtctgca gcagcatcat gagcagcggg tttaagatgc
     6721 tccacacata gccgagcacg ctgcggcggt atttgacctt gaggtcgcgt gtgaccagct
     6781 cccggatgag cggccgatat ttggaaaagt tgcgccagct gttatggaac tggccggtgg
     6841 atttttgcat agggatattg gctccttagt agtatgggga taatgtctcc ctttgacaag
     6901 ggaggctttt ggtggttaca tcttaaacgg tgcatcatcg gtcaggccgt cggcccgcag
     6961 catattctgc atcacatcga tgtcggcgct gacgtcgagg gcgtcgctgg cgaacaggcg
     7021 gtcgagctgg ttttcaaacg ctttgaccag cgtatccatc gtcagctcaa tgcggcgctt
     7081 cgactccgaa atgttctcac cttccacgcc ctggttgtcc agctcagcgt aggtgttcag
     7141 cagctttaac gacgtaggaa ggtaataatc catgaacttg cgcatctggg gcagcttgtc
     7201 ggggtcggac ttggcctgct caaaaatttt ggccgagacc gcttccagac ggctgatctt
     7261 gtcggtcatc tcctcgtcgg ggatggcgtc gttgacccgg cgcagctcgt cgaggatttt
     7321 ctggtactgg tccttctcgg gcatcggatc tgctttttcg accttgggtt tcttcgcctt
     7381 tttcggggtc ggctcggggg gcttgccggt cacgacgaga catttgcggc gcatatccag
     7441 gtaggcttcg gggccgaaca cgccgtcgtc gatgcagttt tccaggtgct tgcagcattt
     7501 ttcatagctg cacgggatgg cgtcggcgat atccttaatg tacatatact gcgcatcacc
     7561 gacaatattg tcgatctttt tgcgcaggcg gcggccggtg cggatgtggt gtgcgccgaa
     7621 caggcagccc gcgcccgccg tggccgagac aaacacaggc atcatatccg ccaagaacca
     7681 gcggatgtac tcgagatccc ccgtcagagc gtcgggcagc cagtaggtac ctccgggcag
     7741 cgccgccagc gcggtcagca gcagcaaaac gcccgccgcg gtcaagccgt tggcaagggc
     7801 catgcccttg cgctttttct cggcatcccg aggtgcggcg gcagttttgc ccgcgctctc
     7861 cttatcctgc tgggcgtaga tagggcggaa ctgtccctcc tgcaccgatg ggccgctgcg
     7921 gtagctctgg gtccgggtac gggctttctg ctcaaagctg cccagcttac cgtttttcat
     7981 agcggcattc aggccgatga agataaagcc cagcggccac cacaccacaa agccaatgac
     8041 gatggcccac caggggaact ggaacgggcc gtccccggcg tggtattcat tgcggttgta
     8101 gttgttctgg tagcgcaggc cgctgccgga gtagggatgg tccgggtccg tgtagcgcgg
     8161 atgttcgttt tcgttgggca tgggtggctc ctttacggaa tatcgttcag caaattcttc
     8221 atatcatcgc ttaaatagta ggtgcttccg gcctggtagg ggttgttctt gctgtatggg
     8281 acgtgggtac acaggtagct gacccacttg ctgtacccgg acaccgtcag gtgtacgccg
     8341 tcgggctgag agaaatcggt ctgcaaatac ccggcatcgt cggcgaattc tgcgttcagg
     8401 tcgaggtaat aacagccgcg ctcggcgcaa agctgctgga tggccgcgtt gatggccgcc
     8461 agccgatccg gtgccaggcc cgggaggctg tcgctgacct tttcctgtgt ggcagtcagc
     8521 accgactgca catagaacgt cgtgtcaggc agcgccgtgc gcaggtcgtc cagcattttt
     8581 ccgtagtagg ccaaaaagct gtcatcgttg ccgggctgga cgagggcgtt tgtaccgatc
     8641 aaaatataca gcttggcggg ttgggcggcg gccagcacat ccagcgggac ttcctcgccc
     8701 cggtcggggc tggtgacggt ggtacggttg acaacggtgt tggggctgat gccctcatac
     8761 ccacagatca gcgcaccacc cacatcaatg ttgtactcat tggcgcagaa gccagcggtg
     8821 agcgagtcac ccaggaaggc tgcgtcggcg aaccaactgg tatccacccg gccaaactcc
     8881 ggcagggcgg cgggctttgc gggcagcacc gtgtaggtgt agccggtatc agcggtctgg
     8941 cgctgcgggc cgacggtgcc ccagttcagc gtctgggcgt tctggctgcc gctgtccccc
     9001 gccccagcgt agggcagcgg ggccaccagg cgggtgcctg cggggtctgc ggtgggcaga
     9061 ggcgcggctt cggtctcgcc cttcatgctt ttgggcagca gcaccgtgat gataccggac
     9121 agcaccagca cgccgcccac cgtgcccagc acggccagcc tgcggcgtcg gcggcgaatt
     9181 tcccgccgtc ggcgggcggc ggcgcggcgc tgttcctgcg tgggggcgcg ccgctggggc
     9241 ggggccgtgg ggtgctggtt gggcacagcc caatcgccct gcatcgtgta gcgttctgta
     9301 tcggggttgc gcgggcggcg gcccggctcc ctgttttgcg gggtcggcat agcggatgcc
     9361 ccctttcatg gcaacatagt aaatcagtat tgattatagc acaaaccttt tataaataac
     9421 agtaggcgcg cagaagtttt taccccttgc taaatgcagt tgtacacggt ataatagtaa
     9481 aatagttaaa aatagcattg ccgggcgctt gtgggggtcg atgcgagcat ccacccctgc
     9541 ggtgtggcgc tgcggcatat aatttggggt gtaatcatgg cagacgaaaa attttccaac
     9601 tttttgacgg atatcatcga cgccgacctc gctgagggca aggtcgatgt tgtgcatacc
     9661 cgcttcccgc cggagccgaa cggctacctg cacatcggtt ccgccaaggc gatcttcatc
     9721 aactatacca tcgccaagca ctacggcggc ctgttcaacc tgcgctttga cgacacgaac
     9781 cccgcccgcg agggtgacga gtacgtgcag agtatcctgc aggacctgaa atggctgggt
     9841 gctgagccga acggcggcat tttctacggc agtgattatt ttgagcgctg ctacgagtac
     9901 gccgagcagc tcatcaagga gggcaaggcc tatgtggacg acctgacccg cgagcagatg
     9961 caggagtacc gcggcaacga cgccggcaag cccagccgcc ccagccccta ccgcgaccgc
    10021 acgccggagg aaaacctcga cctcttccgc cgtatgcgcg cgggcgagtt tgccgacggc
    10081 gaaaagacgc tgcgcgccaa gatcgacctg gcaagcccga acatgaatat gcgcgacccg
    10141 accatctacc gcatcaagca cgtcgagcat caccgccagg gcgacaagtg gtgcatctat
    10201 ccgatgtacg acttcgcaca cccgatccag gacgccatcg agggtatcac cttcagcctg
    10261 tgcagcctgg aatttgagaa ccaccgtccc ctgtacgagt gggtcatcac caatctgttc
    10321 ggcaaagagt tcccgaagca gcgcgaattt gcccgcctga acgtgacgaa caccgtcatg
    10381 agcaaacgct acctgcgtga gctggtggag aaaaacatcg tggacggctg ggacgacccc
    10441 cgtatgccca ccctgtccgg tctgcgccgc cgcggctaca cgccgacgag catcttcaag
    10501 ttcgttaagg acgccggcat ctccaaggcg gataacctcg tcgatatgcg ccagctggag
    10561 gccgtgctgc gtgccgagct ggagctgaac gcccagcgcc gcgtggccgt gctggacccc
    10621 gtcaagctca tcatcgacaa ctaccccgcc gaccagtgcg agtattttga cctgcccaac
    10681 aacccgaacc gcgacgccaa cgactcaacg acccgcaagg tcgccttcac caaggagctg
    10741 tggatcgaga acagcgactt ctttgaggtg ccgccgccga agttcaagcg cctgaccctg
    10801 ggcagcgaag tccgcctgat gggtgcgtac ctggtccgct gcaccggcgt ggacaaggac
    10861 gagaacggca aggtcgtggc gatccacgcc gaggctgacc tggagacccg caacggcaac
    10921 cccgccgacg gccgcaaagt gcgcggcacc atccactggg tcagctgcgc ccactgcctg
    10981 gacgccgagg ttgagctgta cgacaaactg ttcaccgagt ccaacatgaa cagcatcccc
    11041 gacgatgccg acttcaagga ttacctgaac ccggacagcg tcaaggtact gcagggtgcg
    11101 aagctcgagg agagcctgaa ggacgccaag cccggcgacc gcttccagtt tgtgcgcaac
    11161 ggctacttta cgccggacag caagcacgag ggcgtctaca accgcattgt cacactgaag
    11221 gatagcttta agctgtaatt tgtaaaaatg acccccatcg gaaattttcc cgatgggggt
    11281 tttgttgtta cgctttctgt tttttctccc acttctctgc gcagcgctgc atccagccgc
    11341 ccttgaggta gtacacaata aacagcgcgg atgccagcgt ccaggtgatg ggataactca
    11401 actccaccgt ggcgacctcg tggcggatcg gcacggccac agacagccac agcatccgca
    11461 ggccgcagac aaaaaacacc gtgatcagcg tcgggatgcg cacatcgccg cagccgcgca
    11521 gcgccccggc caacacctcg atgcagatat aggtgatgta caccggcgtc aggacgtgca
    11581 tcatgccgac gcccacggcc acgaccgcgt catcctggct gaacagccgg aacagcggct
    11641 ccgccaggtt gtagaacgta acgctgatga gcagcgtgat gcccgccgcc atggcaaggc
    11701 agacccgcac gccgcgccgc acgcggtcgt actgccgtgc gccaaagttc tgccccgcaa
    11761 aggtcgtgat ggcgatgccc agcgagttga tcgtcatcca gaacacaaaa tcgatcttgc
    11821 cgtagacacc ccacgccgca atcgtgctgg tgccgaagct gttcatgctg ctctggatga
    11881 aaatgttgct gatgttgtac atggcgctct gggctgcagc gggcacgccg atggtgcaga
    11941 tggccgcgaa cacatcgccg tgcagccgca gtttttcggg ccggacgtgc cagggcatcc
    12001 cctgggagcc gacgatgcac cgcagcgtca gcgccgcgct ggctacctgg ctgagtaccg
    12061 tggcccacgc cgcgcccgca acgccccagc cgaacaccgc gataaacaca acgtccagcg
    12121 cgatattgac gagcgacgcc gccatcaaaa agtacagcgg ccgcttggaa tcgccgatgg
    12181 cacgcaggat attggtaccc atgttgtaga gcatctgggg gatcatgccc aaaaagtaga
    12241 tgcgcagata cagcgctgca tcgtccagta tttccgcagg cgtacccatc aggtgcatca
    12301 tcggctcagc ggtcagcagg cccagcaccg tcagcagcgc accgatgacc acgctgagca
    12361 ccagcgccgt gtgtacctgg cggtcaacgg cgtcggcatc gtgggcaccc cagctttggg
    12421 caatgacaac gcccgcgccg ctgcacaggc cgacgaacag gcccaccaac agattgacaa
    12481 aagagctggt tgcgcccacc gctgccagcg cgttggtgcc cacaaagttg ccgacgatca
    12541 gagtatccgc cgtgttgtac agctgctgga acagcgtacc gaactagatg ggcagaaaga
    12601 acaacagcag ctgtttgggg atgctgccgg tcaaaatatc cgtttgcttg acacttctca
    12661 tagatttctc tcacttttct gtttctcgcg tccctatttt atgctataat ggcggtaatg
    12721 taaagggggc ttttttgtga cggttttctg ggagccatgc gcgggcggtg tacgggtgct
    12781 gcgcctgctg ggggataccc cctgccccgc cgtacccggc gttattggcg gtctgcccgt
    12841 tgtggagatc ggcccctact gctttgcgga aaagcctgtc aggcccgggg cgcaatgcac
    12901 cggcaccgac acccacgaga tcaccggcaa ttttgtggag gaagtcactc tgccggacac
    12961 ggtgcgggtc ttgcacagcg cagcatttta caactgccgc aagctgcgcc gggtcacgct
    13021 gggggccggg gtggcatcct tgggcagcga tttgttcacg aactgccgcc agttacgggt
    13081 gtttacgctg cgggccgcag cggacgcccc caccgggctg aaaaagctgc tgggg
//
LOCUS       DBMDSQ010000028        13113 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_1443_length_13113_cov_1.950430, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000028 DBMDSQ010000000
VERSION     DBMDSQ010000028.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 13113)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 13113)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13113
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1443_length_13113_cov_1.950430"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..835
                     /locus_tag="ACZPAX_02000"
     CDS             <1..835
                     /locus_tag="ACZPAX_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004066990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HIR1112704.1"
                     /translation="DLMTAVGTIIGNNCLSANIACLYGPGNNIHRTPYGGRNFEYYSE
                     DGFLSGMMSAYEVAAIQEKGVHVVMKHFALNDCEQDRIGLGVWINEQAAREVYLKAFQ
                     APIEVGNGNGVMIAYTRWGAVWSGGNAGLVNGILRGEWGCDGMVITDNVLNVYVNGPD
                     GVLAGVSIYDAMMPYVTDKLPEYKNDGVIVSAMREACHHNLYAIANSCGMNGVGANTT
                     IKLTRPTVITMVIIITCAAAFFCLLGIVLWIFGVRKLRKTEEYKAYKSFLKDQKAAKK
                     A"
     gene            complement(1042..2391)
                     /locus_tag="ACZPAX_02005"
     CDS             complement(1042..2391)
                     /locus_tag="ACZPAX_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005933394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1112705.1"
                     /translation="MVSNKSDFTQGNILHKLTLFMLPILGALVLQAAYGAVDLLVVGH
                     FGTTAGLSGVSTGSQVLNLVTFVVTQLAMGVTVLIGRYLGEKHPEQIGAVLGGAAFVF
                     SILAAVLFVVMVVFAQPIAVLMQAPEAALGQTVNYVRICGCGIFFIVAYNLLSAAFRG
                     LGDSRSPLIFVLVACIVNIVGDLVLVAGFGLDAAGAALATVLAQAISVVCALVMLRRK
                     DLSFTVRRADVRLNPQCRKFLSIGLPLALQEFLTQFSFLALCAFVNRLGLEASSGYGV
                     ACKIVNFSMLIPSALMQSMASFVAQNVGAGNQRRAKQSMYTGLGIGLVIGCAVFALVF
                     WKGDVLAGLFTKNAAVVANAFAYLKGFAPETIGTAILFTMLGYFNGNNQTVFTMAQGI
                     IQTLLVRLPLAYYMSIQPNANLTNIGIAAPVSTGVGVLLCVGFYVWWNRKLAKQSKV"
     gene            complement(2378..2833)
                     /locus_tag="ACZPAX_02010"
     CDS             complement(2378..2833)
                     /locus_tag="ACZPAX_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005933396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIR1112706.1"
                     /translation="MTVKLIKQMIDACRLGKRACDMMPPLPKDVLPSYIHVLDTVHTL
                     EAASGPVRVSDISDALGLPRPGVTRTVKEMEAKGYLTKSASPDDGRVTYITATEAGRQ
                     LCDKYDTQYFSELLPALQDIPEEDAACMIRTIETLYQVLAERRNNVGKQ"
     gene            3148..3711
                     /locus_tag="ACZPAX_02015"
     CDS             3148..3711
                     /locus_tag="ACZPAX_02015"
                     /inference="COORDINATES: protein motif:HMM:NF024183.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HIR1112707.1"
                     /translation="MAFFDDLSKKITDASTKTIQKGKDLTDVARLNSQISAEENRINT
                     LYGMIGKQYMELHRKDAEDAFAGLVASVAEAEQNIAACRAKITELRAVPHCPNCGAEL
                     VKGAAFCGICGTPIPVAPAPAPVAVPAPQQPVQAAPVAPAPAVPVEAPTTPVQSAPAA
                     PAVKFCPNCGVKMPADAQFCAECGTRF"
     gene            3736..4149
                     /locus_tag="ACZPAX_02020"
     CDS             3736..4149
                     /locus_tag="ACZPAX_02020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1112708.1"
                     /translation="MQKAKLGISVGLLGAAIYLTALSGSLLGLLVLAGYVLLVEDNQW
                     LRLSAVKAVAVYLFFTFVIQAVGYVPDVLGIISDAGNLFNAYVSVPVLNDIVTLVKDL
                     IYLAEKIFLMVLGVKALNQGTVAVPFVDKLVVKYL"
     gene            4435..6093
                     /locus_tag="ACZPAX_02025"
     CDS             4435..6093
                     /locus_tag="ACZPAX_02025"
                     /inference="COORDINATES: protein motif:HMM:NF024637.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HIR1112709.1"
                     /translation="MFCGNCGKPIAYANAKFCPACGAPVRVQTDAANEPPKVPVPPRP
                     EEPETPAVPVVEPTPATVGQDAPIPPQPAAATPEKPQAATVSEPEYPDDDATRIVTPA
                     PEPDEDEGATRIFGFDEPAAPIAEQPAAAPQPETPPAPEATAPLFASAAPSDPNPVSV
                     GPNSNAAPIPPQSAAATPEPPQAAPAPNPAPAQSPFPQSVQPTQPITPNPIWQTPPEP
                     EVFSGAKKEKKPGNGPLIFVIVLAVLILAGGGLYFANDYFMLGLEKIPQKLGLVEEIV
                     PDETTGETALPAAEAPTGGSNADTPAAEPTAQPAQTPAPAATAAPTTAPQAVAATADS
                     VVLHGVPDGATITINGQNAAATVQNGDAALSKTALGDTAQLRVIANQGGNWRAAAIWY
                     TADQGSEITLSDSDFLYSGSEDGYLEPDDAFLENVALNYYKAFLNAVNDHDLSEMRFS
                     TARNTDEQSSHVFSDLNAGNTYDTSTASCTLDGSSITWGTYSLTVNVSYMCVREDRDT
                     AVREINSNHRTICMVWQDGMWQVDDIAFLSDDDFAAHKYADLNK"
     gene            6107..8308
                     /locus_tag="ACZPAX_02030"
     CDS             6107..8308
                     /locus_tag="ACZPAX_02030"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HIR1112710.1"
                     /translation="MDFMLGSDYENIRPLDAKGGMGDLFRAHKKGLDVEVVIKRIKRK
                     YIGRLDQQNEANILKGLKHQYLPRIYDVIPGEDGCLYTIMDYIPGVDLQQYVKQNGRV
                     PQPLAHRWACQLCEVVAYLHAQTPPILHCDIKPHNIMITGQGDICLIDFNTSLVFQGD
                     DTVVGVTKGYAAPEQYLAAPTGAVGQPAAPTVSPTAATVMAAPTEYAAPAPLSSAPSA
                     APSNPLVRRSSQPSFSAAVTASAGAYGPLSVRTDVYAIGATMYYMVTAATPERSLDPV
                     TDVTAHRPAISRTMVSIIRRAMSKQQKNRFASADEMLRALQDVDKLDRRYRHFVAARR
                     ATVGVLCAAWLASAGLTLYGANLLRGERINNYLEQVAEGRAQDEDGQYAAADATLRAA
                     VDTMPRRVEGYTALATVFYHRGDYQSAVDLAEGALTSEMLDPAKDTAADGEFYCILAN
                     CYYELGQYAESVTAYQKAIRRDDTNARYYRGLAMAQAKTGDESAAIATLNTAQALTLG
                     DADRDGVLAELYALQGDTAQAWTMYRQVLDAETDNNALSRAYLAAAQLYTSGGVVQTD
                     HLAEVIPLLEEAAQRLPTGYTLAHRQLLATAYGEAAAAWPDTAADSLQKGIAVLQQIE
                     ADGQGGLTTGLSLYAMQHKAGDDTAAEATLLNLQQAYPSDYRPDMELAFLYADMQSKK
                     DAAARDYTAVAACYDTAKQKYQQAAANGANDMSMTELENLMAQLRTAGWLN"
     gene            8320..8460
                     /locus_tag="ACZPAX_02035"
     CDS             8320..8460
                     /locus_tag="ACZPAX_02035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1112711.1"
                     /translation="MLTTGQIVFAVGLAGLAASLIATIVTTKTYHKNEKKLREDIWRE
                     YK"
     gene            8601..12929
                     /gene="essC"
                     /locus_tag="ACZPAX_02040"
     CDS             8601..12929
                     /gene="essC"
                     /locus_tag="ACZPAX_02040"
                     /inference="COORDINATES: protein motif:HMM:TIGR03928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EssC"
                     /protein_id="HIR1112712.1"
                     /translation="MLTLLSVYSESARQDFLLPAAENVTLCARADRFGLGRDIALTLS
                     AAGGWHFTGPCTASLRHVGIPYAGQPVQAGDSLAYCLSGRVALVLLVRQQTSPFAAYD
                     RYRVRPGSIYIGSAADCGLQFTFSSGGQQYITQYHAELRFDGTSFGIYDRSRNGVFVN
                     GRRVRGSAALAFGDEVDIWGLALVILNGGLAVRRTPGLTVAPGVLTPGDQPAVPLGAA
                     LPQTVCHRAPRSLAPLVAAPITPEAAPAGDAGFLSAILPGRRAEQQTAHTAWLARVAA
                     QLQDRCDANTRVMHAQYPSAAHCLQDALAGRHLWQRNPAQADFLTCRAGLGDVPSGAA
                     LALPADAPAELYELQKKYAVLHNVPVALDLAREPLVGLVGGPNRAGAWTMLQSLAAQL
                     AVQCCYTDVKFAFLLRADAADRGDFCRWLPHVRAENGLRLAACGREEARTVCAALAAA
                     LRVPDRPHYVVFVEETALLAGESLAAALLAPAADSGVTAVFLADTAAELPNACGCILE
                     QTTSGAGVLRVHGITTPLTALDAADPAQLDAAVRRLARTVVSDAPAAQALPERVDFFA
                     LYGVHSPAELRAADRWQAARPWETLSVPIGVAADGLCALDAHEQAHGPHGLVAGTTGS
                     GKSETLQTYILSLAVAFSPADAAFFLIDYKGGGMANLFAGLPHVQGAVSNLSGALVRR
                     AMAAIESEIRRRQRIFERCSVNSINQYTPLCKNGEAETPVPHLFIIVDEFAELKREQP
                     EFMRKLISVAQVGRSLGVHLILATQRPAGNVDENILSNTRFRLCLRVQDKQNSMEVLH
                     RPDAAALTGAGRGYLQVGSDEVFTLFQSGWSGAPAAQTAPLAKRIDTTGRPVQSATKA
                     APAPGAATQLSATVDYLRTEAERLHLPALPPLWLPPLPARLVLPAAPQWPEQPARWEL
                     SAIVGRIDDPENQCQLPLVVNFTAGGHHALVGAPGSGKSTFVETLLHALARRYTPDAL
                     NIYALDCTSRRTAAFAACPQVGAVLDATDLSRVGRLFALLDGLLTERQTRLQGGNYTQ
                     YVAAHDVRGCPAVLLVVEDIAAFRERTACAYDDALLRLAREGAACGIYLFVTGSGFGM
                     QDIPARLADALRTVLVLELPDRARYGETLRCRMPQGLPTEAAPGRGLTVLGGRVVEFQ
                     TALAVDAPDDYARADALRRESEALRTAWPGRPARRVPGIPAAPTWRDLSAHEDYAAIQ
                     TAGVCLPFGYDLETAALWGVDLQTTFCFAAAGRAGSGRGNALKILMRSAAAQKARIFV
                     LSSQNDGLHAEADTLGAEYATGADAVQTLLATTIGPEFGRRSQLQKTLPGDPDARWAA
                     MRQEPLWLVVLLDLTDLLRLQTPAGRYCQAVLVNLISRGAGNHIVFAAALDPADAPDS
                     EAYRAFVRHCTGVWLGGGAAQQTLWDFAPLPYRELAAPEKPGVGLVPPTAGHGCQKIL
                     IPQAKG"
BASE COUNT         2486 a         4420 c         3993 g         2214 t
ORIGIN      
        1 cgacctgatg actgcggtcg gcacgatcat cggcaacaac tgcctgagcg ccaacattgc
       61 ctgcctgtac ggcccgggca acaacatcca ccgcacccct tacggcggcc gcaactttga
      121 gtattactcc gaggacggct tcctctccgg tatgatgagc gcctacgagg ttgccgccat
      181 ccaggaaaag ggcgttcacg tcgtcatgaa gcactttgcg ctgaacgact gcgagcagga
      241 tcgcatcggt ctgggcgtct ggatcaacga gcaggctgcc cgcgaagtct acctgaaagc
      301 cttccaggct cccatcgagg tgggcaacgg caacggcgtt atgattgctt acacccgctg
      361 gggcgcggtc tggtccggcg gcaacgcggg cttggtcaac ggcatcctgc gcggcgagtg
      421 gggctgcgat ggtatggtca ttaccgacaa cgtgctgaac gtctacgtca acggcccgga
      481 cggcgtgctg gcgggcgtgt ccatctacga cgctatgatg ccctatgtca ccgacaaact
      541 gcctgagtac aagaacgacg gtgtcatcgt cagcgccatg cgtgaggcct gccatcacaa
      601 cctgtacgcc atcgccaaca gctgcggtat gaacggtgtc ggcgcaaaca cgacgatcaa
      661 gctgacccgc ccgacggtta ttacgatggt cattatcatc acctgcgcgg cagcgttctt
      721 ctgcctgctg ggcatcgtgc tgtggatctt cggcgtaaga aaactgcgca agaccgaaga
      781 atacaaggca tacaagagct ttttgaaaga ccagaaggcc gcgaaaaaag cgtaatccgc
      841 gctgaatagc gcagaaaaac actacccgag gggattgaac ccttcaggtg gtgttttttg
      901 gtggggcggc tcggggaaaa atccccccag cccgcttaac gcgggcagcc ccttttgaca
      961 aggaaacctt tttaaagcct cccttgtcaa agggaggtgg acgccgtgga cggccggagg
     1021 gattcttctg ggggccgacc cttacacctt cgactgcttc gccagcttcc ggttccacca
     1081 cacgtagaaa ccgacacaga gcagcacgcc tacccctgtc gagacagggg cggcaatgcc
     1141 gatgttcgtc aagttcgcgt tgggctggat gctcatgtaa taggccagcg gcaggcgcac
     1201 cagcagcgtt tggatgatgc cctgcgccat cgtgaatacg gtctgattgt tgccgttaaa
     1261 gtaacccagc atcgtgaaaa gaatcgctgt tccgatggtt tccggtgcaa accccttcaa
     1321 ataggcaaag gcgtttgcca cgaccgccgc gttcttggtg aacagcccgg ccagcacatc
     1381 acccttccag aacaccagcg caaacaccgc gcagccgatg accagcccga tgccaaggcc
     1441 ggtgtacatc gactgcttgg cccgcctctg gttgcccgcg ccgacgttct gtgccacgaa
     1501 ggacgccatc gactgcatga gcgcactggg gatgagcatg gaaaaattga cgattttgca
     1561 cgcaacaccg tacccggacg atgcctccag tcccagtcga ttgacgaagg cacagagtgc
     1621 caaaaaggag aactgggtca aaaattcctg caaagccagc ggcaacccga tggacaaaaa
     1681 cttgcggcac tggggattca gccgcacgtc ggctcggcgc accgtgaaag acaggtcctt
     1741 ccgccgcagc atgaccagcg cacagacgac actgatggcc tgggccagca cggtagccag
     1801 tgccgcaccc gccgcgtcca ggccgaaccc ggccacgagt acaagatcac ccacgatgtt
     1861 gacgatgcag gcgacgagaa cgaaaatcag cggcgaacgg ctgtcgccca ggccgcggaa
     1921 cgcagcggac aataaattgt acgccacaat aaagaaaatg ccgcacccgc agatgcgcac
     1981 gtagttcacg gtctggccca gcgcggcctc gggggcctgc atcagtacgg cgatgggctg
     2041 tgcaaacacg accatgacga cgaacagcac ggcggccaga atcgagaaaa cgaacgcggc
     2101 accgcccaaa acagcgccga tttgttcggg gtgcttctcg ccaaggtagc gcccgatgag
     2161 cacggtcacg cccatggcaa gctgcgtgac gacgaaagtg acgaggttga ggacctggct
     2221 gccggtggat acgccggaca ggcccgccgt ggtgccaaaa tgcccgacga cgagcagatc
     2281 gaccgcgccg taggcggcct gcaggaccag cgcacccaga atgggcagca tgaacagggt
     2341 cagcttgtgc aggatgttcc cctgggtaaa atcagattta ttgcttacca acgttatttc
     2401 tcctttcggc taagacctga tacagcgtct cgatagtgcg aatcatgcag gcggcatcct
     2461 cctccgggat gtcctgcaag gcaggtaaaa gctcggaaaa gtactgtgta tcgtacttat
     2521 cacacagctg gcggcccgct tcggtggcgg tgatgtaggt gacgcggccg tcgtcggggc
     2581 tggccgattt ggtcaggtag cctttagcct ccatctcctt gacggtacgg gtcacgccgg
     2641 gccgtggcag gcccagcgcg tcgctgatgt cggacacgcg cacaggcccg ctcgccgctt
     2701 ccagcgtgtg gacagtgtcc agaacatgaa tatacgacgg cagaacgtct tttggcagcg
     2761 gcggcatcat gtcgcacgcc cgcttgccca aacggcacgc gtcgatcatt tgcttgatga
     2821 gcttgacggt catagggggg ctccttctgt gtagattgat gattaccaaa ggtaattacc
     2881 aaagataatt atagacggcg ttttcgattt gtcaagaggc agttgacatt ttgtcgtggg
     2941 taggcatact tgagctagat aacgctaact gtgtagagcg ttgcaggcga tggcatgatg
     3001 aagatgccca tcgtcaaaat ggagcttggg gccgtataaa atcaactgca tttcccctcg
     3061 agaaaatccc aataagcatt gacaaaataa ggcgcaattt gtattataat aggtaaagag
     3121 aatttccaga aagggcggga aaatgacatg gctttttttg atgatctgag caagaaaatc
     3181 accgatgcca gcacgaaaac gatccagaag ggcaaggact tgacggacgt cgcgcggctc
     3241 aactcgcaga tttcggcgga ggaaaaccgc atcaatacgc tgtacggcat gatcggcaag
     3301 cagtacatgg agctgcaccg caaggacgcc gaggatgcct ttgcggggct ggtcgcctct
     3361 gtggccgagg ctgaacaaaa tatcgcagcc tgccgtgcaa aaatcacgga actgcgcgcc
     3421 gtgccccact gcccgaactg cggtgccgag ctggtcaagg gcgctgcgtt ctgcggcatc
     3481 tgcggtacac ccatccccgt tgccccggcc cctgcgccgg tggctgtgcc cgccccgcag
     3541 cagcctgtcc aggccgcccc ggtggccccg gcacccgctg tcccggtgga ggcaccgacg
     3601 actccggtgc agtccgcacc tgcggctccg gctgtcaaat tctgccccaa ctgcggcgtg
     3661 aaaatgcccg ccgatgcgca gttctgcgcc gagtgcggca cccgcttcta agctagagaa
     3721 aagaggaaaa taacgatgca aaaagcaaaa ttgggcattt cggtgggcct gctgggcgcc
     3781 gccatctacc tgaccgccct gtccggcagc ctgctgggtc tgctggtgct ggccggttac
     3841 gtgttgctgg ttgaggataa ccagtggctg cgcctgtccg ctgtcaaggc ggtggccgtc
     3901 tacctgttct ttacctttgt cattcaggcc gtgggctatg tgccggacgt gctgggcatc
     3961 atcagcgacg ctggcaatct gttcaacgcc tacgtcagcg tgcctgtgct gaacgatatc
     4021 gtgaccctgg tcaaagatct gatttacctg gccgagaaga tcttcctgat ggttttgggc
     4081 gtgaaagcgc tgaaccaggg caccgtggcg gttcccttcg tggacaagct ggtcgtcaag
     4141 tacctgtaag ccgcctttac ttacgccatt cattatatcc atatccatcc atcatccggg
     4201 ccgtttcaaa ttcctgggcg gcccgggaaa gcgtccgttg acctgcatcc agctgggaag
     4261 atcgcctgcc acgcaggaca acgatcttgc cgaggggcag ggcagtgggc gttttttgtt
     4321 ttgagaatcc gtaggggcgc acctgtgtgt gcgcccggtt cccggggcgt cgggggacgc
     4381 cgacccctac atatgtgcgc ccggaaaatt gtattgataa aaggggagac cgttatgttc
     4441 tgtggaaatt gtggcaagcc gattgcctat gccaatgcga aattctgccc ggcctgcggt
     4501 gcgccggtgc gcgtccagac cgacgcggcc aatgagccgc ccaaggtgcc cgtgcccccg
     4561 cgcccggaag agccggaaac gcccgccgtg ccggtagttg aaccgacccc ggcaaccgta
     4621 ggacaggatg cccctatccc gccgcagcct gctgcagcca ctccggaaaa accgcaggct
     4681 gccaccgtgt ctgaaccaga atacccggac gacgacgcga cccgcattgt gaccccagcc
     4741 ccggaacccg acgaggacga gggtgccacc cgcatcttcg gcttcgacga acccgccgca
     4801 cccatcgccg aacagcccgc tgctgcgccc cagcccgaaa cgcccccggc ccccgaagcc
     4861 accgccccac tgttcgcatc ggccgcgccg tctgacccca accctgtgag cgtagggccg
     4921 aatagtaacg ctgcccccat cccgccgcag tctgccgcag ccaccccgga acccccgcag
     4981 gccgcccctg caccaaaccc cgcgcctgcc caatctccct tcccgcagtc tgtccagccc
     5041 actcagccca tcacccccaa ccccatctgg cagactccgc cggaacccga ggttttttcc
     5101 ggcgccaaaa aagagaaaaa gcccggcaac ggtccgctca tcttcgtcat cgtgctggct
     5161 gtgctcatcc tggcgggcgg cgggctctac tttgccaacg actacttcat gctgggcctg
     5221 gaaaaaattc cccagaaact cggcttagtt gaggagattg tccccgacga gaccaccggc
     5281 gaaaccgcct tgcctgccgc cgaggccccg acagggggga gcaacgccga cacccccgcc
     5341 gccgaaccca ccgcacagcc cgcccagacc ccggcccccg ccgccacggc ggcccccacc
     5401 acagcgccac aggccgtcgc cgcgaccgct gacagcgtgg tgctgcacgg cgtgcccgac
     5461 ggtgcgacca tcaccatcaa cggccaaaac gccgccgcca ccgtgcagaa cggcgacgcc
     5521 gccctgagca agaccgcact gggcgacacg gcccagctgc gcgtcatcgc gaaccagggc
     5581 ggcaactggc gtgccgccgc gatctggtac accgccgacc agggcagtga aatcaccttg
     5641 tccgacagcg actttttgta cagcggcagc gaggacggct acctggaacc agacgacgct
     5701 ttcctggaaa acgtcgcgct gaactactac aaagccttcc tcaacgccgt taacgaccac
     5761 gatttgagcg agatgcgctt ctcgaccgcg cgcaacaccg acgagcagag cagccacgtg
     5821 ttcagcgacc tgaacgccgg caacacctac gacacctcca ccgcgtcctg cacgctggac
     5881 ggcagctcca tcacctgggg cacgtacagc ctgaccgtca acgtcagcta tatgtgcgtg
     5941 cgcgaggatc gcgacaccgc tgtgcgggag atcaacagca accaccgcac catctgcatg
     6001 gtctggcagg acggcatgtg gcaggtcgat gatatcgctt tcttgagcga cgacgacttt
     6061 gccgcccaca aatacgccga tttgaacaag taaaagggga aaccacatgg actttatgct
     6121 gggcagcgac tatgaaaata tccgcccgct ggacgccaag ggcggcatgg gcgacctgtt
     6181 ccgcgcccac aaaaagggcc tggacgtcga ggtcgtcatc aagcgcatca agcgcaaata
     6241 catcggccgc ctggatcagc agaacgaggc caatatttta aagggcctca agcatcaata
     6301 cctgccccgc atctacgacg ttatcccggg cgaggatggg tgcctgtaca ccatcatgga
     6361 ctacatccct ggtgtggatc tgcagcagta tgtcaagcag aatggccgcg tgccgcagcc
     6421 gttggcccat cgctgggcct gccagctctg cgaggtcgtg gcctacctgc acgcccagac
     6481 gccgccgatt ctgcactgcg atatcaagcc gcacaacatc atgatcaccg ggcaggggga
     6541 catctgcctg atcgacttca acacctcgct cgtgttccag ggcgacgaca ccgtcgtcgg
     6601 cgtgaccaag ggctacgccg cgcccgagca atacttggcc gcgcccaccg gggcagtggg
     6661 gcagcccgcc gccccgaccg tatcccccac ggcggccacc gttatggccg ccccgacgga
     6721 gtatgccgcg cctgcgccgc tgtcgtcggc cccctccgcc gccccctcta atccattggt
     6781 gcgccgcagc tcccagccgt ccttctcggc ggcggtcacg gccagcgccg gggcctatgg
     6841 cccgctgagc gtgcgcaccg acgtgtacgc catcggcgca acgatgtact acatggtcac
     6901 agccgccacg ccggagcgtt cgctcgaccc ggtcaccgac gtgaccgccc atcgcccggc
     6961 catcagccgc acgatggtca gcattatccg ccgcgccatg tccaagcagc agaaaaaccg
     7021 ctttgccagc gccgacgaga tgctgcgcgc cttgcaggat gtggacaaac ttgaccgccg
     7081 ctaccgccat tttgtggcgg cgcggcgtgc cacggtggga gtgctgtgcg ctgcctggct
     7141 tgccagcgcc ggcctgacgc tgtacggcgc gaatctgctg cggggggagc gcatcaacaa
     7201 ttacttggag caggtcgccg agggccgtgc ccaggacgag gatgggcagt acgccgccgc
     7261 ggacgcgacc ctgcgcgccg ccgtggacac gatgccccgc cgcgtagaag ggtacaccgc
     7321 gctggcgacg gtgttctacc accgaggcga ctaccagagc gccgttgacc tggccgaggg
     7381 tgcgctgact tccgaaatgc tggatcccgc caaggacacc gctgccgacg gcgagttcta
     7441 ctgcattctg gccaactgtt actacgagct gggccagtac gccgagtctg tgaccgctta
     7501 ccagaaggcc atccggcgcg acgatacgaa cgcccggtac taccgggggc tggcaatggc
     7561 ccaggccaag accggcgacg agagcgcggc catcgccacg ctgaacactg cccaggccct
     7621 gacgctgggc gacgccgacc gcgacggcgt gctggccgag ctgtacgccc tgcaaggcga
     7681 caccgcccag gcgtggacga tgtatcgcca agttttggac gccgagaccg acaataatgc
     7741 cctgagccgc gcgtatctgg cagcggcgca gctttacacc agcggcggcg tggtgcagac
     7801 tgaccacctt gccgaggtca tccccctgtt agaggaagcc gcccagcgcc tacccaccgg
     7861 ctacacgctg gcccaccgcc agctgttggc gaccgcctac ggcgaagccg ccgcagcctg
     7921 gccggacacc gccgccgatt ccctgcaaaa gggcattgcg gtcttgcagc agatcgaggc
     7981 cgacggccag ggcggcctga ccacgggcct gagcctgtac gccatgcagc acaaggccgg
     8041 ggacgacacc gccgccgagg ccacgctgct gaacttgcag caggcctacc ccagcgacta
     8101 ccgccccgac atggagcttg cgttcctgta cgccgatatg cagagcaaaa aggacgctgc
     8161 ggcgcgggat tacaccgccg tggccgcctg ctacgacacg gcgaagcaga aataccagca
     8221 ggccgcggcg aacggcgcga atgatatgtc gatgaccgaa ctggaaaacc tgatggccca
     8281 gctgcgcacc gcaggctggc tgaactaagg aggaccgcca tgctgacaac cggacagatt
     8341 gtgtttgcgg tggggctggc gggcctggcc gcctcgctga ttgccaccat cgtgaccaca
     8401 aaaacctatc ataagaacga gaaaaaactg agggaagata tctggcggga atataaataa
     8461 ggtgcgcccc aaaacatcgc ggccatgcag gggccgggcg cttggcggcg cacaacgagc
     8521 gccgccagtt tgcttcgcaa ccccgcaacg ccgcccccta caatgctccc cacaaaaatc
     8581 cacacattcg aggtcctacc atgctgactc tcttatcggt atacagtgaa tccgcccggc
     8641 aggacttttt gctgcctgct gctgaaaatg tcaccctctg cgcccgcgcc gaccgcttcg
     8701 gcctggggcg ggacatcgcc ttgacattgt ctgcggcggg cggctggcat tttaccggcc
     8761 cctgcaccgc cagcctgcgc catgtgggca taccctacgc gggccagccc gtgcaagcag
     8821 gggacagcct tgcctactgc ctttccgggc gggtggcgct cgtgctactg gtccgacagc
     8881 agacgtcgcc ctttgccgcc tatgaccgtt accgcgtgcg cccgggcagc atctacatcg
     8941 gcagcgcggc ggactgcggg ctgcagttca ccttttccag cggcgggcag cagtacatca
     9001 cccagtacca tgccgagctt cgttttgacg gtacatcgtt cggtatttac gaccgcagcc
     9061 gcaacggcgt ctttgtcaat ggccgccgtg tgcgcgggtc ggcggcgctg gccttcggcg
     9121 atgaggtcga tatctggggc ctcgcgctcg tgattttaaa cggcgggctg gcggtgcggc
     9181 gcacccccgg cctgaccgtg gcccctggcg tgctcacccc cggggaccag cccgcagtgc
     9241 cgctgggcgc ggctctgccc caaaccgtct gccaccgtgc cccccgcagc cttgcgccgc
     9301 tggtcgccgc gcccatcacg ccggaggccg cccctgccgg ggatgcgggc tttttgtctg
     9361 ccattttgcc gggccgccgc gccgagcagc agaccgccca caccgcctgg ctggcccggg
     9421 tggccgcgca gttgcaagac cgctgcgacg ccaacacccg cgtgatgcac gcgcagtacc
     9481 cctcggccgc gcactgcctg caagacgcac tggccgggcg gcatctgtgg cagcgcaacc
     9541 ccgcccaggc agactttttg acctgccgcg ccgggctggg ggacgtgccc tccggggccg
     9601 cactggccct gcccgccgac gcccccgccg agctatacga actgcagaaa aaatacgctg
     9661 tgctgcataa tgtgcccgtc gccctcgacc ttgcgcggga gccgctggtg ggcctggtgg
     9721 gcggcccaaa ccgcgccggg gcttggacca tgctgcaaag cctggcggcc cagctggctg
     9781 tgcagtgctg ctatacggat gtaaaattcg cctttctgct gcgggccgat gccgccgacc
     9841 gaggggattt ctgccgctgg ctgccccatg tccgcgccga aaatggcctg cgcctggccg
     9901 cctgcgggcg agaggaagcc cgcaccgtct gcgctgcact ggccgccgcc ctgcgcgtgc
     9961 cggaccgccc ccactatgtc gtttttgtcg aggagactgc cctgctggcg ggggaatccc
    10021 tcgccgccgc actcttagcc cctgccgcag attccggcgt tacagcggta tttttggccg
    10081 acaccgctgc cgaactgccc aacgcctgcg gctgcattct cgaacagacc acctccggcg
    10141 cgggcgtgct gcgtgtccac ggcatcacta cgccgctgac cgcgctggat gccgccgacc
    10201 cggcccagct ggacgccgct gtgcgccgcc tggcccgcac tgtcgtcagc gatgcccccg
    10261 ccgcgcaggc cctgcccgag cgcgttgact tttttgcgct ctacggcgtg cacagccccg
    10321 ccgaactccg cgccgccgac cgctggcagg cagcccgccc ctgggagacg ctgtccgtgc
    10381 ccatcggcgt ggcagcggat ggtctgtgcg ctttggacgc acacgaacag gcccacggcc
    10441 cccacggcct ggtggccggc acgaccggta gcggcaaatc cgagacgctg caaacctaca
    10501 ttctctcgct ggccgtggcc ttcagccctg cggatgccgc cttcttcctc atcgactaca
    10561 agggcggcgg catggcaaat ctcttcgccg ggctgcccca tgtgcagggg gcggtctcca
    10621 acctgtccgg ggcgctggtg cgccgtgcca tggcggccat cgagagcgag atacgccgac
    10681 ggcagcggat ttttgaacgg tgcagcgtca acagtatcaa ccaatatacg ccgctctgca
    10741 aaaacggcga ggccgagaca ccagtgccgc acctctttat cattgtggac gagtttgccg
    10801 agctcaagcg cgagcagcct gagtttatgc gcaaactcat cagcgtggcg caggtgggcc
    10861 gcagcctggg cgtacatttg atcttagcca cccagcgccc tgcaggcaat gtggacgaaa
    10921 atattttgag caacacccgc ttccgcctat gtctgcgtgt gcaggacaaa cagaacagta
    10981 tggaggtgct ccatcgcccc gacgcggcgg ccctgaccgg ggccgggcgc gggtatctgc
    11041 aggtgggcag cgacgaggtg tttaccttgt tccagtccgg gtggagtggt gcgcccgccg
    11101 cgcagaccgc cccgctggcc aagcgcattg acacgacagg ccgcccggtg cagtccgcca
    11161 caaaggcggc ccccgctccc ggcgcggcca cccagctttc cgccacggtg gactatctgc
    11221 gcaccgaggc tgagcgtctg cacctgcctg cattgccgcc gctctggctg ccgccgctgc
    11281 ccgcccggct ggtgctgcct gccgccccgc aatggccaga acagcctgcg cggtgggagc
    11341 tttctgccat tgtgggccgc atcgacgacc ccgaaaacca gtgtcagctg ccgctggtcg
    11401 tcaactttac ggcgggcggc caccacgcgc tggtgggcgc accgggcagc ggcaaaagca
    11461 cttttgtcga gacactgctc cacgccctcg cgcggcggta cacgccagac gccctcaata
    11521 tttacgcgct ggattgcaca tcccgccgca cagcggcctt cgcggcctgc ccccaggtgg
    11581 gcgcggtgct ggacgccacg gatctgtccc gcgtcgggcg gctcttcgcg ctgctggacg
    11641 gcctgttgac cgagcgccag acccgcttgc agggcggtaa ttacacccag tacgtggccg
    11701 cccacgacgt tcgcggttgc cccgccgtgc tgctggtggt ggaggacata gccgctttcc
    11761 gcgagcggac ggcctgcgcc tacgatgacg ctctgctgcg cctggcgcgg gagggtgcgg
    11821 cctgcggcat ctacctgttt gttaccggca gcgggttcgg gatgcaggac atcccggccc
    11881 ggctggccga tgccctgcgc accgtgctgg tgttggaact gccagaccgc gcccgctatg
    11941 gcgagaccct gcgctgccgc atgccccagg gcctgcccac cgaggcggcc cccggccgcg
    12001 gcctgactgt gctgggcggc cgcgtggtgg agttccagac cgccctggcc gtcgacgccc
    12061 ccgacgatta cgcccgcgcc gatgcgctgc ggcgggaatc cgaagccctg cgcaccgcct
    12121 ggcccggcag acccgcccgc cgtgtgccgg gcatccccgc cgcgcccacc tggcgcgacc
    12181 tgagcgccca cgaggactac gccgccatac agacggcggg ggtgtgcctg ccctttgggt
    12241 acgacctcga aacggcggcg ctctggggtg tcgatttgca gactaccttc tgctttgccg
    12301 ctgcgggtcg cgcgggttcg ggccggggga acgcactaaa aatcctcatg cgctctgccg
    12361 ctgcacaaaa ggcaagaatt ttcgtattgt cgtcgcaaaa tgacggcctg cacgccgaag
    12421 ccgacaccct gggcgcggag tatgccactg gtgcggacgc cgtgcaaacg ctgctggcaa
    12481 cgaccatcgg ccccgagttt ggccgccgca gtcagctgca aaaaacgctg cccggcgacc
    12541 ccgacgcccg ctgggccgcg atgcgccaag agccgctgtg gctcgtcgtg ctgctggatt
    12601 tgaccgatct gctgcggctg caaacgccag cgggccgcta ctgccaggct gtgctagtga
    12661 acctcatcag ccgcggcgcg ggcaaccaca ttgtgtttgc ggcagcgctt gaccccgccg
    12721 atgcacccga cagcgaggcg taccgcgcct ttgtccggca ctgcaccggc gtctggctgg
    12781 gtggcggtgc ggcccagcaa acgctgtggg actttgcgcc gctgccttac cgcgagctgg
    12841 ccgcgccgga gaaaccgggc gtcggccttg tgccgcccac agccggacat ggctgtcaaa
    12901 aaatcctgat tccacaggcg aaagggtgaa cctattatga tccgcgtcgt tgtctacttg
    12961 agcggaaaag ccgagtgcca actgctggag acccagctgc gccagctcct atcccgccgc
    13021 agcaatgagg aactggactg ccgtttttat gcagaccccg ccgccgcccg cgcggccctg
    13081 ctggccgaga gcatttcctg ggcgataaaa cca
//
LOCUS       DBMDSQ010000029        13054 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_1451_length_13054_cov_2.056203, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000029 DBMDSQ010000000
VERSION     DBMDSQ010000029.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 13054)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 13054)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13054
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1451_length_13054_cov_2.056203"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(101..562)
                     /locus_tag="ACZPAX_02045"
     CDS             complement(101..562)
                     /locus_tag="ACZPAX_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HIR1112713.1"
                     /translation="MPVWTHYFAHFVVAVIATIAFGITFQVPRRHYLAAGITGAVGWM
                     VYIFGVELLSMSPAIATLVATLPLTGCARFFSVTHKAPVTIFLLPGIFPLVPGAGIYY
                     TAYYFLQGEQELFASKGGETFKVALALALGIALICSLPLPGGHEKIKRPSP"
     gene            complement(562..1335)
                     /locus_tag="ACZPAX_02050"
     CDS             complement(562..1335)
                     /locus_tag="ACZPAX_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015536905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HIR1112714.1"
                     /translation="MAEDLFDRALELVMDGGQTLLANGGEVFRVQQTMEIMAASLGVQ
                     DFHVYVLTNGIFASAHLPGGESVTLVRHVPTVSIHLGRVEAVNELSRELAAGKLGVVE
                     AEQKLAAARALPRVTPRREVLACIAGAAGFAYLFGGTLADVPVAAVAGLLEALVCLHF
                     GRHSINRMFTDIVAAFCCTVWAIAVQAVVPAVSANAAIIGALMVLTPGVALTMGVRDI
                     LNGDYLSGSIRLLDALLIAGSIAGGVVLGWIVARGLGVA"
     gene            complement(1328..2089)
                     /locus_tag="ACZPAX_02055"
     CDS             complement(1328..2089)
                     /locus_tag="ACZPAX_02055"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HIR1112715.1"
                     /translation="MKRLLITICGRAGSKGFKNKNLKNFCGKPLVYYSLSAAELFIRN
                     HPELEVDIALNTDSEDLAKLVAAEYPEVIYLPRGAELGGDRVPKVEVYKDSFARMEAR
                     TGKPYDAMIDLDITSPLRTEQDIENAFAKAQEREDLQIIFSVCEARRNPWFNMFKIVG
                     DHAEMVMESQFTGRQQAPEVYDVNASIYVIRRAFLVDNPDPILWHSKFGVSVMMDTGI
                     IDIDSEHDYLLMEAIAQHLYAHYPEFNAVRENIRG"
     gene            complement(2091..3041)
                     /locus_tag="ACZPAX_02060"
     CDS             complement(2091..3041)
                     /locus_tag="ACZPAX_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR1112716.1"
                     /translation="MRTITALFVGLGSIGTRHLKNLANLCADRGWALQADALRSDLSR
                     PLRDGVAELLHAQYTDMADAPAHYDLVFITNPTSLHADALTQVKGRGEALFIEKPIFS
                     AEQTGLALANYLPTGQKAYVAAPMRWCGVMLALKDRLPALHPYCARVICSSYLPDWRP
                     GVDYRTVYSAHKALGGGVTIDLIHEWDYLVELFGVPEKLYNFKGTYSDLEIDSDDLSV
                     YIAKYPTLLAEVHLDYFGRGYRRSIELFCHDGSYLADFGAGTLTLPDGTVQHYEEDVN
                     RRYEREMEYFVDYALTGSGESCNPPALALKVLKLTLGENV"
     gene            complement(3106..4083)
                     /locus_tag="ACZPAX_02065"
     CDS             complement(3106..4083)
                     /locus_tag="ACZPAX_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR1112717.1"
                     /translation="MKLLIVGLGSMGKRRARLTKGIDDAIQIVGVDTAEGRRDEAKNL
                     GLADAAYATIAEAVAAEHPDAALVCTAPLSHAAVIGELLDNDLPVFTELNLVSDGYAE
                     NMAKAAAKNLPLFLSSTMLYRRETQYIKRQVTEFGKPVHYIYHIGQYLPDWHPWENYK
                     NFFVGNARTGGVREIFGIDLPWLLDAFGDVESVTVQKDTLSDLRLPYPDSVTLLLRHK
                     GGVQGVLAADVVSRKAVRSFECFGDGIHLFWEGNPKALYEFRDGDKQPVDTYASFEHD
                     SRYSDNIVENAYVDELTNFFGVLKGEESPKWSFEKDLKAIALMDQIENS"
     gene            complement(4080..5288)
                     /gene="neuC"
                     /locus_tag="ACZPAX_02070"
     CDS             complement(4080..5288)
                     /gene="neuC"
                     /locus_tag="ACZPAX_02070"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0006047 - UDP-N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HIR1112718.1"
                     /translation="MKKLRCDMRTICIVTATRAEYGLLRPVVQKVAASDELDLQLVVT
                     GAHLCPRLGETVHEIENDGFPIAARLPIFTDDVDEPVACTIARTINVFDSYFAAHRPD
                     AVLLLGDRFEIFAVAAAAAARHIPIAHISGGDVTLGAADEYYRHCISKMAAVHFPSCA
                     DSAARLVRMGEAPDTVFCVGGLGDENIRTLPKMSREELCKSTGFDLMQPFALVTYHPE
                     TAPDAGSPAVQVRALCSAMDKVDSVFWLITGSNADAGGQVCTEMMQAFAASHPGRAGF
                     VQSLGLRRYLSAMDCAALVAGNSSSGVVETPTFKVPTVNIGKRQAGRMVCANVLCCAP
                     DAAAIEATMRKALSPSFAPLAASAVSPYGGGDTSGKICAVLQHFDFTKSKVFYDGPVP
                     EFDPQRSVLV"
     gene            complement(5269..6264)
                     /gene="neuB"
                     /locus_tag="ACZPAX_02075"
     CDS             complement(5269..6264)
                     /gene="neuB"
                     /locus_tag="ACZPAX_02075"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HIR1112719.1"
                     /translation="MPVTIIAEAGVNHNGSLEMAKEMARAAKECGADIVKYQTAVPEL
                     VVSKFAEKAEYQKQTTDAAESQLEMIRKLHFSFEEHKELKEYCDSIGIQYLSAPFDIP
                     SVRFLGTLGLPLIKMPSGEITNLPYLEEMAKLHTPVLLSTGMSTLDEITDALGVLDDG
                     GCPEVTVLHCNTQYPTPYEDANLTAMLELYEQFGLPVGLSDHTPGWECDVAAAVLGAQ
                     VIEKHFTLDKSLPGPDQKASLDPAEFKAMVNAVRHVEAALGDGHKHLTASEAPNKAIA
                     RKSIVAAREIKAGEVFTEDNLTTKRPGDGISPMRWHEVLGQTAKRDFAEDEKIEV"
     gene            complement(6401..7450)
                     /locus_tag="ACZPAX_02080"
     CDS             complement(6401..7450)
                     /locus_tag="ACZPAX_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HIR1112720.1"
                     /translation="MIQVEELFIEPTATVLETLRKLDVTGQRILFIAPEGKLKAVITD
                     GDIRKFFLRGGTPDQTVDHAANYHPLSVPIAERGRAREILQKNCIDALPVLNKRGVIT
                     DIIFAHGLNVDNRKQVDIPVVMMAGGLGTRLYPYTKILPKPLIPVGEQPIAELIMDRF
                     RDFGCHEFTMIVNYKKGMIKSYFGELEKDYRVDFADEDVFMGTGGGLCLLKGKIDTPF
                     FFTNCDTLLDVDFGDIYEYHKSHGNLVTMICAFKHYTVPYGVVELGEDGSIAAMREKP
                     ELDFLTNTGVYVVEPRVVEEMHDGEFIGFPDVIERYRRAGEKVGVYPISESSWMDMGQ
                     LEELEKMRRKLENQQ"
     gene            complement(7660..8823)
                     /locus_tag="ACZPAX_02085"
     CDS             complement(7660..8823)
                     /locus_tag="ACZPAX_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LegC family aminotransferase"
                     /protein_id="HIR1112721.1"
                     /translation="MSGFIPLSVPNFGVREAELAGQAITSGWVSTSGGKVTEFEEALA
                     AYVGMPRAVACNSGTSALHLAAMAVGITRGDEVIVPTLTFIAAVNPLTRYVGAEPIFI
                     GCDDSLCIDPDAVEDFCANHCELRGDKLYNKKTGAHVKALEVVHVFGNMADMPRLTAI
                     AKRYHLILIEDATEALGTRCTTGPFAGKFAGTIGDIGCYSFNGNKIITTGAGGMVVSN
                     HPDWAEHAKHLSTQAKADVLQFLHDEVGYNYRMTNVQACLGLAQLERLEDFIAHKKAL
                     YDRYAAALDGVKGLRILPFREEECRSNHWFFSLYLEDSGLDRDTVIEKLQAQQIQTRP
                     VWALIHEQADYPRNEAYGLDKALAYRKYIVNLPCSTNLSFEDCDRVIEAVLGL"
     gene            complement(8968..9456)
                     /locus_tag="ACZPAX_02090"
     CDS             complement(8968..9456)
                     /locus_tag="ACZPAX_02090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1112722.1"
                     /translation="MAAGPAADFFHAERPLVFVPIIKESIAGCKASFCVQEDGTVYDY
                     EAETKTESGIVKTITFVNPDGFSEFTVMANKTVTVTIDGKEYALSAADIGKGFEIAKK
                     DFCDWKGVSFKGIEGVYTTLTKELYDKAANGQKLEGTYAPTASSAKTAAPCTPAHGAA
                     AF"
     gene            9424..10512
                     /locus_tag="ACZPAX_02095"
     CDS             9424..10512
                     /locus_tag="ACZPAX_02095"
                     /inference="COORDINATES: protein motif:HMM:NF024136.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3810 domain-containing protein"
                     /protein_id="HIR1112723.1"
                     /translation="MKKICRWACRHRAGLLALAVGFAAWGLFYIARRSTAAMDWWLTY
                     VSMPVKRGLSFIVDPLPFSACELGATVLIVGALVLLARAVRRRRLAAWALHMAVLLVW
                     GYAGVCALWGTQYYGTNFAARAGMTAPPISTGELAAVTDYFADKVNAAAPLVQRDESG
                     VFATDKTEIFAGCAHLYDPLTERWPFLAGPERRPKPAVYSKLMSAWGFTGYLCPLVGE
                     STLNVDSPAVFLPVTIAHELAHQRGVAAEQEANFVGVMAATASANADYAYSGWLFGYL
                     HLSNALYEADPALAAASYQTLCAEARADLAANNAYWKQWEGPVKQTGEKVYTTFLQGY
                     GQDLGMRSYGACVDLLVEEFLTNTTSCD"
     gene            10709..12829
                     /locus_tag="ACZPAX_02100"
     CDS             10709..12829
                     /locus_tag="ACZPAX_02100"
                     /inference="COORDINATES: protein motif:HMM:NF013357.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH25 family lysozyme"
                     /protein_id="HIR1112724.1"
                     /translation="MFNKKNPMRYFSGSTFARLPLYKLAAGGTAVALTASIGLGAFAA
                     GVTPFNLPTATPAPVVTATPETAATPTPSPSPTPSPTPVPDRTLTMEATVVQQDVGVT
                     LYVDPLPEATATPEPTALPSASPKAGASAASGAKPTATPKATATPTPTPTAAPTEDDE
                     QEKTPLLGVEGTITLTDAKGNATDYALDPETGTALAEDVEPGDYTLALQPIEGYTTPE
                     AQTVTVKEKVVYKADVQAVKEKIVQASQVNESAEDSGVSNAGSAPIVEEVTDTVTYAE
                     SDKTETGTKTTYTAKLSSSGHLLLADGTETPYLPVYQDGTRDLIGATRDPNYAANTNT
                     SAWLPGAADSIQLLGAANTRMAWAHRADEPAVQEGDEAASIAEETPAPTEAPTPEPTA
                     EPTAEPTAEPTAEPTAEPTPEVTATPEPTAAPTNTPAPTEAPTATPDPTAGWPDNIEA
                     KDLASYGFDVTSSDGTVYQYTGWQEIDGVTYYYDPTTHQPVTGQQVIQGDVYTFGADG
                     ALNRTARGIDVSKFQGSIDWNAVKADGITFAIIRCGYRGYGSGALVEDSTYRANIQGA
                     INAGLKVGVYFYSQAINEQEAVEEASMVLSLVGGYSLPLGVYYDTESVAGGRANAISA
                     AERTACAVAFCETIRNSGYSAGVYSYASWFYNALNFANISKYNIWIAQYRDTLSFSYK
                     YNIWQYTGSGSVKGISRPVDMNIG"
BASE COUNT         2530 a         4084 c         3969 g         2471 t
ORIGIN      
        1 ggactgatga ggggcgggct ggccgttact gcgctttacc atgcaataac ggctgacccg
       61 cccctcatcc ggccatcgct tcgctcggcc accttctccc tcagggagaa ggccttttta
      121 ttttctcatg cccgcccggc agcggcaagc tgcatatcag cgcaataccc agggccagcg
      181 ccagggcaac cttgaacgtc tcgccgcctt tgctggcaaa caattcctgc tcgccctgca
      241 aaaaatagta ggccgtgtag tagatgcccg cgccgggcac aagcgggaag atgcccggca
      301 gcagaaaaat cgtcaccggg gctttgtgcg tgacggaaaa gaaccgcgca cacccggtca
      361 gcggcagggt agccaccagc gtggcgatgg ccgggctcat gctcagcagc tccacgccga
      421 agatgtagac catccagccc accgcgccgg tgatgccggc ggccaggtaa tgccgccgcg
      481 gtacctgaaa cgtgatgcca aaggcgatcg tggcgatgac cgccaccaca aaatgtgcga
      541 aataatgtgt ccagaccggc atcaggccac ccccaatccg cgggcgacga tccagcccag
      601 cactacgccg cccgcaatgc tgcccgcaat cagcagcgcg tccagcaggc ggatggagcc
      661 ggacaggtag tcgccgttta aaatgtcacg cacgcccatc gtcagggcga cgccgggggt
      721 cagcaccatc agcgcaccga tgatggccgc gttggcgctg acggcaggca ccacggcctg
      781 caccgcaatg gcccagaccg tgcagcagaa cgccgccacg atatccgtaa acatccggtt
      841 gatgctgtgg cggccaaaat gcaggcagac gagcgcttcc aacaggcccg ccacggcggc
      901 taccggcacg tcggccagtg tgccgccgaa cagataggcg aaccccgccg ccccggcgat
      961 gcaggccagc acctcccgcc ggggcgtgac ccggggcagt gcacgggctg ccgccagctt
     1021 ttgctcggct tcaacgacgc cgagcttgcc ggcagccaat tcgcgggaaa gctcgttcac
     1081 agcctcgacc cggcccagat ggatggatac ggtgggcacg tgccgcacca gcgtgacaga
     1141 ctccccgccg ggcaggtggg ccgatgcaaa aatgccgttg gtcagcacat agacgtgaaa
     1201 atcctgcaca ccaaggctgg cggccataat ttccatcgtc tgctgcacgc ggaacacctc
     1261 gccgccgttg gcaagcaggg tctgcccgcc gtccatcacc agctccagcg cacggtcaaa
     1321 caggtcctca gccacgaata ttctcccgca cagcgttaaa ttccgggtaa tgggcgtaga
     1381 ggtgctgcgc gatggcctcc atcagcagat agtcatgctc ggagtcgata tcgatgatgc
     1441 cggtgtccat catgacgctg acaccgaact tggaatgcca caggatgggg tccgggttgt
     1501 ccaccaaaaa tgcgcggcgg ataacataaa tcgacgcatt gacgtcgtaa acctcgggcg
     1561 cttgctggcg gccggtgaat tggctctcca tgaccatttc ggcgtggtcg cccacgattt
     1621 taaacatatt gaaccacggg ttgcggcggg cctcgcagac gctgaagatg atttgcaggt
     1681 cctcgcgctc ctgcgccttg gcaaaggcgt tctcaatgtc ctgctcggtg cgcagcgggc
     1741 tggtgatatc caggtcgatc atggcgtcgt agggcttgcc ggtgcgcgct tccatgcggg
     1801 cgaagctgtc cttgtagacc tcgaccttcg gtacacggtc gccgcccagc tcggccccgc
     1861 ggggcaggta gatgacctct gggtattcgg cggccacgag cttggccaga tcctcactgt
     1921 cggtgttcag cgcaatgtct acttccagct cggggtggtt cctgatgaag agctcggcgg
     1981 cgctcaaaga gtagtaaacc aacggtttgc cgcagaaatt tttcaagttt ttgtttttga
     2041 agcccttgct gcctgcgcgg ccgcagatgg tgatgagcag acgtttcatg ttacacattc
     2101 tcccccagtg ttaatttcag caccttcagt gccagtgcgg gcgggttgca gctctcgccg
     2161 ctgcctgtca gcgcgtagtc cacaaaatat tccatctccc gctcatagcg gcggttgacg
     2221 tcctcctcgt aatgctgcac ggtgccgtcg ggcagggtca gcgtgcccgc gccaaagtcg
     2281 gccaaataac tgccgtcatg gcagaaaagc tcgatagaac ggcgatatcc gcgcccaaag
     2341 tagtcgaggt gcacctcggc cagcagcgta ggatacttgg cgatgtagac cgagagatcg
     2401 tcggaatcga tttccaaatc cgagtaggtc cctttgaaat tgtacagctt ttccggcacg
     2461 ccgaacagct ccaccagata gtcccactcg tgaatcaggt cgatggtgac gccgccgccc
     2521 agtgccttgt gggcgctgta cacggtacgg tagtccacgc cgggccgcca gtcaggcaga
     2581 tagctcgagc agatgacccg ggcgcagtag gggtgcaggg cgggcaggcg gtctttcagc
     2641 gccagcatca caccgcacca ccgcatgggc gcggccacgt aggccttttg cccggtgggc
     2701 agatagttgg ctaaagcaag accggtttgc tcggcagaaa aaatcggctt ttcgatgaaa
     2761 agcgcctcgc cgcggccctt gacctgggtc aatgcgtcgg cgtgcaggct cgttgggttc
     2821 gtgataaaga ccaggtcgta gtgcgcgggc gcgtcggcca tgtcggtgta ctgggcgtgc
     2881 agcagctcgg ccacaccgtc ccgcagcggg cgggaaaggt cgctgcgcag tgcgtcggcc
     2941 tgcaaggccc aaccgcggtc agcgcatagg ttggcaagat ttttcaaatg gcgcgtgccg
     3001 atggagccca gccccacaaa cagcgcggtg atggtacgca taggcgttcc cctttgtctt
     3061 gatttagatg caatgtaggg gccgggtatg cccggcccta ccgggttacg aattttcaat
     3121 ctggtccatc aatgcaatgg ccttcaaatc cttctcaaag ctccacttag ggctttcctc
     3181 gcccttcaga acgccgaaaa agttcgtcag ttcgtccaca taggcgttct cgacgatgtt
     3241 gtcactgtat cggctgtcat gctcaaagct ggcgtaggtg tccaccggct gcttgtcgcc
     3301 atcgcggaat tcatacagcg ccttggggtt gccctcccag aaaaggtgga tgccgtcgcc
     3361 gaagcactca aagctgcgga cggccttgcg gctgaccacg tcggcggcca gcacgccctg
     3421 cacgccgccc ttatggcgca gcagcagcgt tacgctgtcg gggtagggga gccttaagtc
     3481 actcaaggtg tccttctgca cggtgacgga ttccacatcg ccgaaggcat ccagcagcca
     3541 aggcaggtcg atgccgaaaa tctcccgcac gccgccggtg cgggcgttgc cgacgaagaa
     3601 attcttgtaa ttctcccacg ggtgccagtc gggcagatac tgcccgatgt ggtagatata
     3661 gtggacaggc ttgccaaatt cagtgacctg acgtttaata tactgcgtct cgcggcggta
     3721 cagcatcgtc gaggagagaa acagcggcag gtttttggcg gcggcttttg ccatgttctc
     3781 ggcgtagccg tcgctgacca ggttcagctc ggtaaagacc ggcaggtcgt tgtccagcag
     3841 ctcgccgatg acggcggcgt gggacagcgg tgcggtgcag accagcgcgg cgtcggggtg
     3901 ctcggcggca acggcctcgg cgatcgtcgc ataggcggcg tccgccagac ccaggttctt
     3961 ggcctcgtcg cggcggccct cggcggtgtc cacgccaacg atctgaatgg cgtcgtcgat
     4021 gcccttggtc agccgggcgc ggcgtttgcc catgctgcct aaaccgacga tgagaagttt
     4081 cataccaaaa cgctcctttg ggggtcaaat tccggcacag ggccgtcata aaaaaccttg
     4141 gattttgtaa agtcaaaatg ctgcaataca gcgcagattt ttccactggt gtcgccgccg
     4201 ccgtaagggc tgacggcgct tgccgccaac ggggcgaagg agggggagag ggctttgcgc
     4261 atcgtggcct cgatggccgc tgcgtcgggg gcgcagcaga ggacgtttgc gcagaccatg
     4321 cgcccggcct gccgcttgcc gatgttgacg gtgggcacct taaaagtcgg tgtctcgacg
     4381 acgcccgatg acgagttccc cgccaccagg gcggcgcagt ccatggcgga aagataccgc
     4441 cgcaggccca ggctttgcac aaagcccgcg cggcccgggt gcgaggcggc gaacgcctgc
     4501 atcatctcgg tgcagacctg cccgcctgcg tcggcgttcg acccggtgat aagccagaac
     4561 acgctgtcca ccttgtccat ggcgctgcac agcgctcgca cctgcacggc gggcgacccg
     4621 gcgtcggggg cagtctcggg gtggtaggtc acaagcgcga agggctgcat caaatcaaac
     4681 ccggtggatt tgcacaattc ttcgcggctc attttgggca aggtgcggat gttttcatcc
     4741 cccagcccgc cgacgcagaa cacggtgtcg ggcgcttcgc ccatgcgcac gagccgcgcg
     4801 gcgctgtcgg cgcaggacgg aaaatgcacc gccgccattt tggaaatgca gtggcggtag
     4861 tattcgtcgg ctgcgcccaa ggtcacgtcg ccgcccgaaa tgtgggcaat gggaatatgc
     4921 cgcgcggcgg ccgcggcggc cacagcgaaa atttcaaagc ggtcgcccag cagcagcacc
     4981 gcgtcggggc ggtgggcggc aaaatagctg tcaaaaacgt tgatagtgcg tgcaatcgtg
     5041 caggcaaccg gctcgtcgac gtcgtcggta aaaattggca ggcgtgcggc gatgggaaaa
     5101 ccgtcgtttt cgatttcgtg tacggtttcg cccagccgcg ggcagaggtg cgcgcccgtc
     5161 acgacaagct gcaagtcgag ttcgtcggac gccgcgacct tctgtacgac aggccgcagc
     5221 agcccatatt ccgcccgcgt ggcggtaacg atgcagatgg tgcgcatatc acacctcaat
     5281 tttttcatcc tccgcaaaat cgcgcttggc ggtctggccc agcacttcat gccagcgcat
     5341 gggggaaatg ccatctcccg ggcgcttggt ggtcaggttg tcctcggtga agacctcgcc
     5401 cgccttgatt tcccgcgcgg cgacgatgct cttgcgggca atggccttgt tgggggcctc
     5461 gctggcggtc aggtgcttgt ggccgtcacc cagggcggcc tccacatggc gcacggcgtt
     5521 caccatggcc ttgaactctg ccgggtccag gctggccttc tggtcggggc cgggcaggga
     5581 tttgtccagc gtgaagtgtt tttcaatgac ctgcgcaccc agcacggcgg cggccacgtc
     5641 gcactcccag cccggcgtat ggtcggacag gcccaccggc agaccaaact gctcgtacag
     5701 ttccagcatg gcggtcaggt tggcatcctc gtagggggtg gggtactggg tgttgcagtg
     5761 cagcaccgtt acctcggggc agccgccgtc gtccagcacg cccagtgcat cggtgatttc
     5821 atccagcgtg ctcatgccgg tggaaagcag cacaggtgtg tgtaatttcg ccatttcttc
     5881 caggtacggc aggttcgtga tctcgcccga cggcatcttg atgagcggca ggcccagcgt
     5941 gcccaaaaac cgcacgctgg ggatatcgaa cggtgcggac agatactgga tgccgatgga
     6001 atcacagtat tctttcagct ccttgtgctc ctcaaacgag aaatgcagct tgcggatcat
     6061 ttccagctgg ctctcggcgg cgtcggtggt ctgcttctga tactcggcct tctctgcaaa
     6121 tttgctcacg accagctcgg gcacggcggt ctggtacttg acgatatccg cgccgcactc
     6181 cttggcagcg cgggccattt ccttggccat ttccaggctg ccgttgtggt tgaccccggc
     6241 ctcagcaatg atggttacag gcattttaaa actccttccg ctgtggcttc tccccttggg
     6301 gagaagctgt ccgcaaagca gactgatgag ggggccttcc ggcaggctgc ccctcatccg
     6361 gcgctgcgcg ccaccttccc ccaaagggga aggcttttat ttactgctga ttttccagct
     6421 ttcgtctcat tttctccagt tcttccagct ggcccatgtc catccagctg ctctcgctga
     6481 tgggatagac gccgaccttt tccccggccc ggcggtagcg ctcgatcacg tcgggaaagc
     6541 cgatgaactc accgtcgtgc atttcctcca ccacgcgcgg ctccaccacg taaacgccgg
     6601 tgttcgtcaa aaagtccaac tcgggcttct cgcgcatggc ggcaatgctg ccgtcctcgc
     6661 ccagctccac cacgccgtag ggcacggtgt aatgcttgaa cgcgcagatc atcgtgacaa
     6721 gatttccgtg agacttatga tactcgtaaa tatcgccgaa gtccacatcc agcagcgtgt
     6781 cgcagttggt gaaaaagaac ggcgtgtcga tttttccttt cagcaggcac agcccgccgc
     6841 cggtacccat gaacacatcc tcatcggcaa aatccacgcg gtaatccttt tccagctcgc
     6901 caaaatagga cttgatcatg ccctttttgt agttcacgat cattgtgaac tcgtggcagc
     6961 cgaagtcccg gaaccgatcc atgataagct ccgcaatggg ctgctcgccc accggaatca
     7021 gcggcttggg caggattttg gtgtaggggt acagccgcgt gcccaggccg cccgccatca
     7081 tcacgaccgg gatatcgacc tgcttgcggt tgtccacatt cagcccgtgg gcaaagatga
     7141 tatcggtgat gacgccgcgc ttatttaaaa ccggcagtgc gtcgatgcag tttttttgca
     7201 gaatctcccg cgcccggccg cgctcagcta tcggcacgct cagcgggtgg tagttggcgg
     7261 cgtggtcaac ggtctggtcg ggtgtgccgc cgcgcagaaa aaacttgcgg atatcaccgt
     7321 cggtgatgac ggctttcagc ttgccctccg gcgcaatgaa caaaatgcgc tggccggtca
     7381 cgtccagctt gcgcagcgtt tccagcaccg tggcggtcgg ctcaataaag agttcttcta
     7441 cttgtatcaa gggtggatcc cccttttacg tttatttaaa gccttccccc ttggggaaag
     7501 gtggcccaaa gggccggatg aggagcggat gtgccgcagc agaccgtgga tgggaaatag
     7561 cggtaaggct gcccctcatc agtcaccttc ggtgacagct tctcccaaag gggggaagcc
     7621 ttggcggggc gttgaggatg ccgcccccta caaaacgtct tacagcccca gcaccgcttc
     7681 gatcacgcgg tcgcagtcct caaagctcaa gttggtggag cagggcagat tgacaatata
     7741 cttgcggtag gccagggcct tgtccagacc gtaagcctcg ttgcgggggt agtcggcctg
     7801 ctcgtggatc agcgcccaca ccgggcgggt ctgaatctgc tgggcctgca atttctcaat
     7861 gacggtgtcg cggtccaggc cggaatcttc cagatacagg ctgaagaacc aatggttgga
     7921 gcggcattcc tcctcacgga aaggcagaat gcgcagcccc ttcacgccgt ccagtgcggc
     7981 tgcatagcgg tcatacagcg cctttttgtg ggcgataaaa tcttccaaac gctccagctg
     8041 ggccaggccc aggcaggcct gcacgttggt catgcggtag ttgtagccca cctcatcgtg
     8101 caaaaattgc agtacatcgg ccttggcctg ggtggacaga tgcttggcgt gctcggccca
     8161 gtccgggtgg ttggacacga ccatgccgcc tgcgccggtg gtgatgatct tgttgccgtt
     8221 gaagctgtaa cagccgatgt cgccgatggt gcccgcaaac ttaccggcaa acggcccggt
     8281 ggtgcagcgg gtgcccaggg cctcggtggc atcctcgatc agaatcaggt gatagcgctt
     8341 ggcaatggcc gtcaggcggg gcatatcggc catgttgccg aaaacatgga cgacttccag
     8401 cgccttgacg tgggcaccgg tctttttgtt gtacagctta tcgccgcgca gctcgcagtg
     8461 gttagcacag aaatcctcca ccgcgtcggg gtcaatgcac agggagtcgt cgcagccaat
     8521 aaaaatcggc tcggccccca catagcgggt cagcgggttg acggcggcga tgaaggtcag
     8581 cgtcggcaca atgacctcat ccccgcgggt gatgccgacg gccatagcgg ccagatgcag
     8641 cgcgctggta ccggagttgc aggccacggc gcggggcatg cccacgtaag cggccagcgc
     8701 ttcctcaaac tccgtgactt tgccgccgct ggtggagacc caccccgacg tgatagcctg
     8761 tccggccagc tcggcctcgc gcacgccgaa attcggaaca gaaagaggaa taaatccaga
     8821 cataacaatg cctccataat acagcagtcg caccggaatt gcccggcgga cagaattcta
     8881 caaatattct attttattat accgctttta cggggagaga gcaagaagaa aatgggctgg
     8941 aaaagcctcc cttgccaaag ggaggcttta aaacgcagcc gccccatgcg cgggtgtgca
     9001 tggggcggca gtcttggcgg aagaagcggt cggggcgtag gtgccttcca gcttttggcc
     9061 gttcgccgcc ttgtcgtaca gttccttggt cagggtcgtg taaacgccct caatgccctt
     9121 gaaggaaacg cccttccaat cgcagaaatc tttcttggca atctcaaaac ccttgccgat
     9181 atctgcggcg ctgagcgcgt attccttgcc atcgatggtg acagtaacgg ttttgtttgc
     9241 cataacggta aactcgctga aaccgtcggg gttgacgaag gtgatggtct tgacgatgcc
     9301 gctctcggtt ttcgtctcgg cctcataatc gtagacagtg ccgtcctctt gaacacaaaa
     9361 agacgccttg cacccggcaa tgctttcctt tataatgggt acaaaaacca gggggcgttc
     9421 tgcatgaaaa aaatctgccg ctgggcctgc cgccaccgcg cgggcctgct ggcccttgcc
     9481 gtgggcttcg cggcgtgggg gctgttctat atcgcccgcc gcagtaccgc cgctatggac
     9541 tggtggctca catatgtttc gatgcccgtc aagcgcggcc tttcctttat agtagacccg
     9601 ctgccgttca gcgcgtgcga gctgggcgca acggtgctca tcgtcggggc gctggtgctg
     9661 ctggcccgcg ccgtgcgccg ccgcaggctg gcggcctggg cgctgcatat ggccgtgctg
     9721 ctggtctggg ggtacgcggg ggtctgcgca ctgtggggca cgcagtatta tggcacgaat
     9781 tttgccgccc gcgcgggcat gaccgccccg cccatcagca ccggggaact ggccgctgtg
     9841 accgactact ttgccgacaa agtcaacgcg gcggccccgc tggtgcagcg ggacgaaagc
     9901 ggcgtgtttg ccaccgacaa aaccgaaatc tttgccgggt gcgcccatct gtacgacccg
     9961 ctgaccgagc gctggccgtt tttggccggg ccggagcgcc gccccaagcc cgccgtctac
    10021 tccaaactca tgagcgcctg gggcttcacc ggatatctct gcccgctggt gggggagagc
    10081 accctcaacg tggacagccc ggcggtgttc ctgccggtga cgatcgccca cgaactggcc
    10141 caccagcgcg gcgtagcggc cgagcaggag gcaaactttg tcggcgtcat ggcggccacg
    10201 gccagcgcca acgccgacta cgcctactcc ggctggctgt tcggctacct gcacctgtcc
    10261 aacgcgctgt acgaggctga ccccgcgctg gcagcggcca gctaccagac cctttgcgcc
    10321 gaggcccgcg ccgacctggc cgccaacaac gcctactgga agcagtggga aggcccggtc
    10381 aagcagacgg gcgaaaaagt ttacacgacc tttttgcagg ggtacggcca ggacctgggt
    10441 atgcgcagct atggcgcgtg cgtggatctg cttgtggaag aatttttgac caatacaact
    10501 tcttgcgatt gacagtgccc cgcaccgcta gatgcccgga aagttgtttc cacaatatca
    10561 caattcttaa caaacggccc acccggatgt tgaaaatttc ggcgtttttc agcacatttt
    10621 tcgtccctcg cccccttgcg gcgggggctt ttttcatggt atgatttatg gtgtagcaat
    10681 ttagaatttt caactgtgag gaacaggaat gtttaataaa aagaatccca tgcggtattt
    10741 ctcaggcagc acgtttgcgc ggctgccgct gtataagctg gcggcgggcg gcacggctgt
    10801 cgcgctgacg gccagcatcg gcctgggcgc gttcgcggcg ggcgttacgc cgttcaacct
    10861 gcccacggcc accccggccc cggtcgtgac cgctacaccg gaaacggcag ccacgcccac
    10921 gccgtcgccg tcccccacgc ccagccctac gccggtgccg gaccgcaccc tgaccatgga
    10981 ggccaccgtc gtgcagcagg acgtcggcgt gacgctctac gtggacccgc tgcccgaagc
    11041 cacagcaacg ccggaaccca cggctctgcc gtctgcgtcg cccaaggctg gcgcatcggc
    11101 ggcgtccggg gctaaaccca cggccacgcc aaaggcgacc gccaccccga ccccgacgcc
    11161 caccgccgcc ccgaccgagg acgacgagca ggaaaagacc ccgctgctgg gcgttgaggg
    11221 caccatcacc ctgaccgacg ccaagggcaa cgccaccgac tacgcgcttg accccgagac
    11281 cggcaccgcc ctggccgagg acgtggaacc cggcgactac acgctggccc tgcagcccat
    11341 cgaaggctac accacccccg aggcccagac cgtaactgtg aaggaaaagg tcgtctacaa
    11401 ggcggatgta caggctgtca aggaaaagat cgtccaggca agccaggtca acgagagtgc
    11461 cgaggactcc ggcgtcagca acgcaggttc cgcgcccatc gtcgaggaag tgactgacac
    11521 ggttacatat gccgagagcg acaagaccga gaccggcacc aaaactactt acacggccaa
    11581 gttgagcagc agcggccacc tgctgctggc cgacggcacc gagaccccct atctgcccgt
    11641 ctaccaggac ggcacgcggg atctgatcgg cgcgacccgc gacccgaact acgcggccaa
    11701 cacgaacact tctgcctggc tgcccggcgc agcggattcc attcagctgc tgggcgcggc
    11761 caacacccgc atggcgtggg cacaccgcgc cgatgaaccc gccgtgcagg agggggatga
    11821 ggcggcctcc atcgccgagg agacgcccgc tcccaccgag gctcccactc cggaaccgac
    11881 ggcagagcct actgccgagc ccaccgcaga gccgacggca gaaccaaccg ccgagcctac
    11941 accggaagtg acggcgacgc ccgaaccgac ggccgccccc acgaacaccc cggccccgac
    12001 cgaggccccc accgccacgc cggatcccac ggcgggctgg cctgacaata tcgaggccaa
    12061 ggatctggcg agctacggct ttgacgtgac cagctccgac ggcaccgtgt accagtacac
    12121 cggatggcag gaaattgacg gcgttacgta ctattatgac ccgacgaccc accagcccgt
    12181 caccggacag caggtcatcc agggcgacgt ttacaccttc ggggcggacg gtgcgctgaa
    12241 ccgcacggcc cgcggcatcg acgtttccaa gttccagggc agcattgatt ggaacgccgt
    12301 caaggccgac ggcatcacgt ttgccatcat ccgctgcggc taccgcggct acggcagcgg
    12361 cgcactggtc gaggattcga cctaccgcgc caatatccag ggcgctatca acgcgggcct
    12421 gaaagtcggc gtctactttt acagccaggc catcaacgag caggaagccg ttgaggaggc
    12481 cagcatggtg ttgagcctgg tgggcgggta cagcctgccg ctgggcgtct actacgacac
    12541 cgagagcgtt gcgggcggcc gcgccaacgc catcagcgcc gccgagcgca cggcctgcgc
    12601 cgtcgctttc tgcgagacga tccgcaactc cgggtacagc gcgggcgttt actcctacgc
    12661 aagctggttc tacaacgcgc tgaactttgc gaacatcagc aagtacaaca tttggatcgc
    12721 ccagtaccgc gatacgttga gtttcagcta caagtacaac atctggcagt acaccggctc
    12781 cggcagtgtc aagggtatct cccggccagt ggatatgaac attggataaa accaaataga
    12841 atagaacttc cccctgcttg cgcagcactg cgcaagcagg gggaagttct ttgataaacg
    12901 gatcgccctt gtgtttgtga tgaacgcaag ggtattttta ctttgtgtag cagctcatct
    12961 ttgcgctgct gccgcctctt gtctggccgc tgccgtggat gatgtacttg taggtacaca
    13021 gctcagccaa ccccatgggg ccgcgggcgt gcag
//
LOCUS       DBMDSQ010000030        12992 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_1465_length_12992_cov_2.427275, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000030 DBMDSQ010000000
VERSION     DBMDSQ010000030.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 12992)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 12992)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12992
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1465_length_12992_cov_2.427275"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            100..291
                     /locus_tag="ACZPAX_02105"
                     /pseudo
     CDS             100..291
                     /locus_tag="ACZPAX_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005927358.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(701..789)
                     /locus_tag="ACZPAX_02110"
     tRNA            complement(701..789)
                     /locus_tag="ACZPAX_02110"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(752..754),aa:Ser,seq:cga)
     gene            1203..2384
                     /locus_tag="ACZPAX_02115"
     CDS             1203..2384
                     /locus_tag="ACZPAX_02115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1112725.1"
                     /translation="MKKTLKHLLPALFLAAVMALLTGCTKSVDLLQYADVTFDGASGN
                     ATATVTLDYNGLGKELFGKGKDQDIWDEAQTELSLMGHVNYTVTPQTGLSNGDTVTLT
                     VSADDAFLKNHEITLSETSKTLTVSGLKEPETIDPFEDSLLNIAVEGETVSGKLNILI
                     RGCAPHLGVTVSSDLPADDPRSALTYSVATESPEGGYAQGDVVTITATPKFSSDFTNN
                     YILSRDSLEVSLENVPHYLNSADEVTPDLLAQLKPLVENELNSSVDYGMTYTSIWAAD
                     GSSPVSSESSASGPRWTGTASLLVDKDSDIYNGSYYGDNMLVFPYEIAYNVFSGEGSG
                     TAVGGIYLLNVKVNADGSYDLSEVSTGTTSYYTDMDAFLAKEVNNRSEWYDITTAPFN
                     W"
     gene            complement(2647..3492)
                     /locus_tag="ACZPAX_02120"
     CDS             complement(2647..3492)
                     /locus_tag="ACZPAX_02120"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR1112726.1"
                     /translation="MPEQPLVSVIVPIYNAQDHIARCVESIRRQTYKNLEILLLNDGS
                     KDVSLEVCKMYANVDPRIVLIDKANSGVAATRNMGLREAKGKYLQFVDADDTIQPYAT
                     EMLVQRAEENGADLVIAHYNRITPPRPRAEDDPRPERPTKVQTYGFLMEGPMTKEEFA
                     AGLMQEPASFYYGVMWNKLYRADIVRAHDDIICAEDMDWSEDLYFNLSYIRYAEKFYA
                     LSTPIYNYFNTPGSAVHTALNPVNVVATRATLFAYYKELYEHLGLYEEYKPQIYKYLV
                     AVAEA"
     gene            3726..5216
                     /locus_tag="ACZPAX_02125"
     CDS             3726..5216
                     /locus_tag="ACZPAX_02125"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: protein motif:HMM:NF014165.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HIR1112727.1"
                     /translation="MKDSIIRLRALEKQLYAYLYALTVIDFDAETIAPEGSADGRAEA
                     TEVLSRANFDLLVNEKTAALLKEAAADAETEQEKAEVRNLQRQYDEIAKIPADEYAAF
                     TKLCSQSVPAWVKAKRTNDFSIFAPYLEKIVAARRAQAHYFAPDRDPYEVLLDRYEKG
                     LTIAQCDEFFATLRETIVPLLADIQARGSAVRTDFLDQEWPIDAQRKVSEKIMQLWGL
                     DPAHSHLSESEHPFTTEFWRGDVRITTHYMARDMFSNLYSVAHEGGHALYELNINPDY
                     DYTAVTHGATMGIHESQSRLFENIVGRSRAFVNYLYPTLKELFPAQLADVSAEEIWRA
                     VNRAEPSLIRTEADELTYALHIMVRYELEKALIQGTLAVTDLPAAWNAKYKEYLGVDV
                     PDNAHGCLQDIHWAMGDIGYFPSYALGSAYGAQAIDDLRKTNDFDAQWAKGDMEPLKA
                     ALKERVWQWGSMKEPQWIVESLCGGKFDPHHFTEYLKKKYTELYNL"
     gene            complement(5256..5819)
                     /locus_tag="ACZPAX_02130"
     CDS             complement(5256..5819)
                     /locus_tag="ACZPAX_02130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1112728.1"
                     /translation="MENVKTNALAASYRADLLYYIERAKLGKCPPHWQAYCFGEIADA
                     KGTDNYPADGDALLAELRQLVDTVPQITNRDENVAEIAAYRGRMLFYFDRDYYTLAEL
                     VHLPGKDKYSACVYIDADGSSSDRTAYAKNIAVQVDEWRRQNDIPFDKSTIAARPADT
                     DGGEFDTMDEALRYLYTCLNFPDSVLC"
     gene            complement(6059..6583)
                     /locus_tag="ACZPAX_02135"
     CDS             complement(6059..6583)
                     /locus_tag="ACZPAX_02135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1112729.1"
                     /translation="MRKLIAVVLGAALLAGCAAAPAASESAAAASGTAQTTPAPTEEP
                     TAAEDPAAYDTTGEQIDVLDQLVDFGADTAGGSLKTAKAAAVLVEYLSCSDLDADVMA
                     DWCTGLTDDQKELLDLNWPGILSEAEAICGDPAGEADELITAGVTTDFENMALTEVPD
                     KLVTVNTVLTGKAG"
     gene            complement(6614..6997)
                     /locus_tag="ACZPAX_02140"
     CDS             complement(6614..6997)
                     /locus_tag="ACZPAX_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003401429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HIR1112730.1"
                     /translation="MHPNEHCNCRYADVGCDKMEEVMDQISGKWKMRILFMVGAHGTL
                     RYGELRKLLDGVTHKMLSNQLKELAADGLVERIDYNEVPPRVEYRLTPNGEALMPIFD
                     DIQRYIRGHACNDCCGGGAKHACCE"
     gene            7229..8527
                     /locus_tag="ACZPAX_02145"
     CDS             7229..8527
                     /locus_tag="ACZPAX_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007048011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HIR1112731.1"
                     /translation="MYDDLVEFLQESVTPFHAAATAESWLRAAGFTRLEEADYWNLEP
                     GKGYYTTRNGSSVVAWRVPDHAIGGWRIVASHSDSPTWKIKTDIVENDGCRRLSVEGY
                     GGMIMSTWLDRPLTVGGRVIVKTEDGIESRLVCIDRDLLVIPSLAIHFQRDINKGHTF
                     NPQVDMQPLWGPAGSRTLTDLVAEELGVDTADILDSDLQLVTRQAPTQIGPDGEFFMA
                     PRIDDLECAATTLLGFLDAAAETDSACAPVWAMLDNEEVGSSSRMGAESSYLRDVLDR
                     IVDAIPHSGQAMHRAMANSFMLSADNAHATHPNFPQKSDPCAPVRLGGGVVLKYNASQ
                     KYTTNAVSGAIFRAICEKADVPVQVFTNRADEAGGSTLGNLQSHTLPIPMADIGLAQL
                     AMHSAVETASVADAEAMTKAVAAFYRVHLRALGDGTYTLA"
     gene            8524..9444
                     /gene="gluQRS"
                     /locus_tag="ACZPAX_02150"
     CDS             8524..9444
                     /gene="gluQRS"
                     /locus_tag="ACZPAX_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014116157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HIR1112732.1"
                     /translation="MITGRYAPSPSGRMHLGNLMCCLLAWLSAKSKGGQVLLRIEDLD
                     TQRCPRAYADAIIDDLAWLGLAADGPEPPIYQSERSAIYQQYFDELARRGLVYPCFCS
                     RSQLHAASAPHRSDGQVVYAGTCRGLTPAEIAARTKTRAPAWRVQVPDEVIAFEDGHM
                     GHYAENLARDCGDFYLRRADGVFAYQLAVVVDDALMGVDEVVRGSDLLSSTPRQLWLY
                     RELGLTAPKFYHLPLLLAPDGRRLSKRDGDQSLENLREKYTAGEIIGKLAFACNLQPK
                     PTPTTPQELARTFDWARVPKSDILLPEKLF"
     gene            9635..12235
                     /locus_tag="ACZPAX_02155"
     CDS             9635..12235
                     /locus_tag="ACZPAX_02155"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007048013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0140358 - P-type transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HIR1112733.1"
                     /translation="MQRYDITGMSCAACASHVEKAVAAVPGVASVAVSLLTNSMQVDY
                     APDADPDATAKRIIKAVDAAGYGASLATAESENAELEDTETPKLKKRLIASLCFLVPL
                     MYVSMGHMIGLPLGSVFHGGGWHAILFAVTQLALTLPVCWINRAFFISGWKGIKTRAP
                     GMDTLVALGAGASLAYGLFAIVMICVGLYTGNDDLVMQYRHDLYFESAAMILTLITVG
                     KTLEAYSKGKTTDALKSLMKLAPQTACVLRDGEQVTVPVSEVNVGDLFLVRPGESIPV
                     DGTVAEGISTVNEAALTGESMPVDKFPGSPVSAATINQNGALTCRAERVGQDTTLSQV
                     IRLVRDAAATKAPLAKTADKVSGIFVPTVICIATATFLIWLLLGQTFTFALARGISVL
                     VISCPCALGLATPVAIMVGSGVGAKNGILFKTAASLETTGYTDTVLLDKTGTVTTGEP
                     SVVKIVGTGNVPEKFLLSLAAGLELRSEHPLARAILERADKDKLSYTTVADFEAVPGK
                     GLQGKVAGKVIAGGNANFIREHCELPDDLTQAGDAMSADGITPLYFSLAGKAAGVIGV
                     ADVVKKTSKSAIDQMKALGLKVVLLTGDNAATARHIGATIGLDADHVIAGVLPAGKEA
                     EVRALQAQGRVAMVGDGINDAPALTRAETGIAIGAGADVALDAADVVLVRSDLADVPA
                     AVRLSRAVVRNIHQNLFWAFFYNAICIPLAAGVFTGIGITLNPMIASAAMSLSSVCVV
                     TNALRLNAFDPRNAAHDAPPKRKAPARKPISEPTTCPTGSCPVQPAEINDKTEEITMN
                     KTIHIEGMMCGHCEAAVKKALEALDGVAGATVSHEQGTAIVALTHDVADAELKAAVEA
                     KDYTVTGIDA"
     gene            12232..12516
                     /locus_tag="ACZPAX_02160"
     CDS             12232..12516
                     /locus_tag="ACZPAX_02160"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004603659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HIR1112734.1"
                     /translation="MSTLRRRWRFTGTVQGVGFRYYARAAALHLGLTGWVANNWDGSV
                     TLEAQGDRAALDALVPLIERTNRWARIENIETAILPLKEHEYGFEERSGE"
     gene            12719..>12992
                     /gene="rsmI"
                     /locus_tag="ACZPAX_02165"
     CDS             12719..>12992
                     /gene="rsmI"
                     /locus_tag="ACZPAX_02165"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006735234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0070677 - rRNA
                     (cytosine-2'-O-)-methyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HIR1112735.1"
                     /translation="MPGTLYLVATPIGNLDDMPPRVAATFGMADFIAAEDTRVTLRLL
                     NHLGLKKPMVSYYEHALHHGEAILQRIEAGENCALCSDAGMPCISDP"
BASE COUNT         2626 a         4172 c         3702 g         2492 t
ORIGIN      
        1 atttttcggt ttggaaaagg atgaaaaaat cagactgaaa acctcaaaac ccttgatcct
       61 acggggcttt tccggtttgt cgtaattata ccgtaagggc actcctgcgc cagcctattg
      121 ctgaaagagg gcgtaccgat gaagcagatt caagagtggc tgggtcacag cgatatttcc
      181 accacggcga atatctacgc gcatctggat tcgcaatcca agaacctgag cgcccggacg
      241 atggcaaaca cgctgacact gcccgaagcg cagcccataa agaagtggta atttgaacag
      301 cggcggcatc tgagaaatca ggtgccgcct ttttgctgtc aatttgtacc caagctgcgg
      361 gaagaatctt gtgcagttct gtgaatagct atttgccgcg attgctggta tgatttgtac
      421 cgaacccgaa aatcactact tgaaaataaa ggggagatgc ttataagaaa agcaacggaa
      481 ttttgcaaac tagcgacaaa aaatcactac ccaatgtaga atcgagtagt gatacttggc
      541 gcagacggtg ggattctccc gcgcccttgc aacagtccgc aggactgttg cagcccggcg
      601 ggacgcaaag cgccgggcac gggctgttcg aatcccacct tgcaaccctt gcatagtttg
      661 ccgcaaaaat aaaaagcagc accccgcagg gtgctgctct tggcgcagac ggtgggattc
      721 gaacccacgt ggcattgctg ccaaattgat ttcgagtcaa tctcgttacg accgcttcga
      781 tacgtctgcc tatttagttt ttatcgcacc gccccgcaca gcgtttggaa aaatgcgaga
      841 tttcgatgca agataacgat gaaaggttgg gtacgcgaca cccgataaag cccgccgtta
      901 tgggatttcc tgcggacaag gtgacctgcc ggatgcaaac tttcgagtgc tccctcttac
      961 gaccacttga gtacatctgc ctgtgtactt taccgcaccc acacagggat acttatagta
     1021 ttataccaac tccccgcccg gttgtcaatc atccgccaag gttttctgcc atttctgcca
     1081 ttcatacgct ttcgatgacc attcatcctg ctttcccccg cagtgacccc acccgtgcta
     1141 tactgtgtac agttcaagca aaacttctcc actccccccc acaaagaaaa ggatggataa
     1201 ccatgaaaaa gacactcaaa catctgctcc ctgcgctgtt cctggcggcg gtcatggctt
     1261 tgctgaccgg ctgcaccaaa tccgtggacc tgctgcaata cgccgacgtc accttcgacg
     1321 gtgccagcgg caacgccaca gccaccgtaa cgctcgacta caacggcttg ggcaaagagc
     1381 tgtttggcaa aggcaaggac caggacattt gggacgaagc ccagaccgag ttgagcctga
     1441 tgggccacgt caactacacc gtcaccccgc agaccgggct gtccaacggc gacaccgtga
     1501 ccctgaccgt aagcgccgac gacgccttcc tgaagaacca cgaaatcacc ttgagcgaga
     1561 ccagcaagac cctgaccgtc tccggcctga aggagcctga gacgatcgac ccgttcgagg
     1621 acagtctgct gaacatcgcc gttgaaggtg aaaccgtgag cggcaagctc aacatcctta
     1681 tccgcggctg cgccccgcac ctgggcgtca cggtctccag cgacctgccc gccgacgacc
     1741 ctcgttcggc gctgacctac tccgtcgcga ccgaaagccc cgagggcggt tatgcccagg
     1801 gcgatgtcgt caccatcacg gccacgccca aattcagcag cgacttcacc aataactaca
     1861 ttctctcccg cgacagcctt gaagtttccc tggaaaacgt tccccactac ctcaactccg
     1921 ccgacgaagt gacccccgac ctgctggccc agctgaagcc cctggtcgag aacgagctga
     1981 acagcagcgt cgactacggc atgacctata ccagcatctg ggccgccgac ggcagctctc
     2041 cggtttccag cgagtccagc gccagcggtc cccgctggac cggcacagcc tcgctgctgg
     2101 tggacaagga cagcgacatc tacaacggca gctactacgg tgacaatatg ctggttttcc
     2161 cctacgagat tgcctacaac gttttctccg gcgagggttc cggcacggcg gtcggcggta
     2221 tctacctgct gaacgtcaag gtcaacgcgg acggcagcta tgatctgagc gaagtctcca
     2281 ccggcacgac cagttactac actgacatgg acgctttcct ggccaaggaa gtcaacaacc
     2341 gcagcgagtg gtacgacatc accaccgcgc ccttcaactg gtaacaactc tacgctccgc
     2401 agattgtgcc gcacagcttt gcgtcagcga caagatcgtc tccaagcggg agactgcccg
     2461 cagcctgccc gacatcaccc tgttggagcc gccggccgcc ggggccatgg gaagctgtac
     2521 tggtactgca gccggcacgg attatttgag caaaaggtat aacaaaagcg agcagccacg
     2581 caggctgctc gcttttattt tgtggccgag ggccgggtat gcccggcccc tacacacggt
     2641 attcttttac gcttccgcca cggccaccag gtatttgtaa atctgcggct tgtattcctc
     2701 gtacaggccc aggtgctcgt acagttcctt atagtaggca aacagcgtgg cgcgtgtggc
     2761 aacaacgttg accgggttca gcgctgtgtg gaccgcactg ccgggcgtat taaaataatt
     2821 gtaaatcggc gtggagaggg cgtagaactt ttccgcatag cggatgtagc tcaaattgaa
     2881 atacagatcc tccgaccagt ccatatcctc ggcgcagatg atatcatcgt gggcgcgcac
     2941 gatatcggcg cggtagagct tattccacat aacaccataa taaaagctgg ccggttcctg
     3001 catcaggccc gcggcgaact cttccttggt catggggcct tccatcaaaa agccgtaggt
     3061 ctgcaccttg gtggggcgtt cggggcgggg gtcatcctcg gcccgggggc ggggcggcgt
     3121 gatgcggtta tagtgggcaa tgaccaggtc agccccgttt tcctcggcgc gctgcaccag
     3181 catttccgtc gcgtagggct ggatggtgtc atccgcatcc acaaattgca ggtactttcc
     3241 cttggcctcc cgcaggccca tgttgcgggt ggcggccacg ccgctgttgg ccttgtcgat
     3301 aaggacgatg cgcgggtcaa cgttggcgta cattttgcag acttccaggc tgacgtcctt
     3361 actgccatcg ttcagcagca ggatttccag gtttttgtag gtctggcggc ggatgctttc
     3421 cacgcagcgt gcaatatgat cctgggcatt gtagatcggc acaatgacac tgacgagcgg
     3481 ctgttccggc atggcgggcg gcctcctttt tacttagtat acccacaata ccataatttt
     3541 gcccggttgg caatggaaaa aatccgccgg gtatggtatg atgaaggtaa aggcttttcc
     3601 cgagggagaa gctgtccgca aagcggactg atgaggggcg cgtttcccac ggctgcccct
     3661 catccggcct gcggccacct tccccaaggg ggaaggcatt tctgttgaaa cgagggacac
     3721 cacacatgaa agactccatc atccgcctgc gtgcgctgga aaagcagctg tacgcgtatc
     3781 tctacgccct gaccgtcatt gactttgatg ccgagaccat cgcacccgag ggttcggccg
     3841 acggccgtgc cgaggctacc gaggtgctga gccgcgccaa ctttgacctg ctggtcaacg
     3901 aaaagacagc tgccctttta aaagaagcag ccgccgatgc cgagaccgag caggaaaaag
     3961 ccgaggtgcg caatttgcag cgccagtacg acgaaattgc caagattccc gccgacgaat
     4021 acgccgcctt caccaagctg tgcagccaga gcgtgcccgc ctgggtcaag gccaagcgca
     4081 ccaacgactt cagcatcttt gcgccgtatc tggaaaagat cgtcgccgcc cgccgcgccc
     4141 aggcccacta ctttgcgcct gaccgcgacc cctacgaagt gctgctggac cgctacgaga
     4201 agggcctgac catcgcccag tgcgacgaat tttttgccac cctgcgggag acgatcgtgc
     4261 cgctgctggc ggacatccag gcccggggca gcgccgtccg caccgacttt ctcgaccagg
     4321 aatggcccat cgacgcccag cgcaaggtca gcgagaagat catgcagctg tgggggctgg
     4381 accccgccca cagccacctg tccgagagcg agcatccctt caccaccgag ttctggcgcg
     4441 gcgatgtgcg catcacgacc cattacatgg cgcgggatat gttctccaac ctgtacagcg
     4501 tcgcccacga gggcggccat gcgctgtatg agctgaatat caacccggac tacgactaca
     4561 ccgccgtgac tcacggcgca accatgggca tccacgagag ccagtcccgc ctgtttgaga
     4621 acatcgtggg ccgctcccgc gcctttgtca actacctgta cccgacgctg aaagagctgt
     4681 tccccgccca gctggccgac gtctccgccg aggagatctg gcgcgccgtc aaccgtgccg
     4741 agcccagcct gatccgtacc gaagcggacg agctgaccta cgcgctgcac atcatggtgc
     4801 ggtacgagct ggaaaaggct ttgatccagg gcacgctggc cgtgaccgac ctgcccgccg
     4861 cctggaacgc caagtacaaa gagtacctgg gcgtcgatgt gcccgacaac gcccacggct
     4921 gtttgcagga catccactgg gccatgggcg acatcggtta ttttccgagt tatgccctgg
     4981 gcagtgccta cggcgcacag gccattgacg acctgcgcaa gacgaacgac tttgatgccc
     5041 agtgggccaa gggcgacatg gagccgctga aagccgcgct gaaggagcgc gtctggcagt
     5101 ggggcagcat gaaggaaccc cagtggatcg tcgagagtct gtgcggcggc aagttcgacc
     5161 cgcaccattt caccgagtat ttaaagaaga aatatacgga gctgtataac ctgtaattga
     5221 aagagccttt tccctaagag gaagcctttt ttatttcagc acagcacact gtccgggaaa
     5281 ttcaggcagg tgtacagata ccgcagggcc tcgtccatgg tgtcaaactc gccgccgtcg
     5341 gtgtccgccg ggcgggcggc gatggtggac ttatcaaacg ggatatcgtt ctgtctgcgc
     5401 cattcatcca cctgcacggc aatgtttttc gcgtaggcag tgcggtcgct gctgctgccg
     5461 tcggcatcaa tgtagacgca ggcgctgtac ttgtccttgc cgggcaggtg taccagctcc
     5521 gccagcgtgt agtagtcgcg gtcaaagtag aacagcatcc tgccgcggta ggcggcaatt
     5581 tccgccacgt tttcgtcacg gttcgtgatc tggggtacgg tgtccaccag ctggcgcagt
     5641 tcggccaaca gtgcgtcacc atcggcgggg tagttgtctg tgcccttggc atcggcaatt
     5701 tcgccgaagc aataggcctg ccagtggggc gggcacttgc ccagtttggc gcgctcgata
     5761 tagtagagca ggtccgcacg gtaagaggct gcaagagcat tggtttttac gttttccata
     5821 agttttctcc taaaaatctg aaagtagttt tgtaccgcag cgctcgcgca cttcctccta
     5881 gtaagtgcgc ggcgctgcct ttttgttttt cctgacagcc ctatcgtatc acactccgct
     5941 gtcgaatctt gccacattat aagaccgaac gccgttttat tttggggaca gttgtcctgt
     6001 gatgtcaaaa aacaaaatgc agaggcataa aacgcacctc tgcatttttg tttgtctatc
     6061 agcccgcctt gccggtcagc acggtgttca ccgtcacgag cttatcgggc acctcggtca
     6121 gggccatgtt ttcaaaatcc gtcgtcacac ctgcagtgat cagttcatca gcctcgcccg
     6181 cagggtcacc gcagatcgcc tcggcctcgc tcaaaatgcc gggccagttc aggtccagca
     6241 gctccttctg gtcgtcggtc aggcccgtgc accagtcagc catcacatcg gcatccaaat
     6301 ccgagcagct cagatattct accagcacgg cagcggcttt ggcagtcttc aggctgccgc
     6361 ctgctgtgtc cgcgccaaaa tccaccagct gatccaacac atcgatctgc tcgccggtcg
     6421 tatcatacgc cgcagggtcc tcggccgcgg tcggctcctc ggtgggagcc ggggtagttt
     6481 gcgccgtgcc ggacgcagct gccgccgatt ccgatgctgc cggggcagcg gcacagccag
     6541 ccagcagggc agcccccagc acaacagcaa tcaatttacg cataaacgat tccccctacg
     6601 cgtttatttc atattattca cagcaggcgt gctttgcgcc gcccccgcag caatcattgc
     6661 aggcgtggcc gcggatatac cgctggatat cgtcaaaaat cggcataagc gcctctccgt
     6721 tgggggtcag gcggtactcg acccggggcg ggacctcatt atagtcgatg cgctccacca
     6781 ggccgtccgc agccagctct tttaactggt tcgagagcat cttgtgggtc acaccgtcca
     6841 gcagcttgcg cagctcgcca tagcgcagcg tgccgtgggc acctaccata aagagtatgc
     6901 gcattttcca cttgccgctg atctggtcca tgacctcttc catcttgtca cagcccacat
     6961 cggcatagcg gcagttgcag tgttcgttcg ggtgcatagg ggttcactct ccttttgggt
     7021 agcggtttcc aaaaagtgcg tacttgtcaa aaccgaccat ttcgctatac tgagtataga
     7081 acaattttcg caggtttgca acttgtttct tacaggcttt tgcggtaata tagttgtgtg
     7141 caactcaagc gtagggcggc atccccatgc cgtcccacgg tatcaccgcc aaccgtgcgg
     7201 aatattgttc aagaggagaa accgacctat gtacgatgat ctcgtagaat ttttgcagga
     7261 atccgtcacc ccgttccacg ccgctgccac ggccgaaagc tggctgcgcg ccgccggttt
     7321 tacccggctg gaagaagccg actactggaa cctggaaccc ggcaagggct actacaccac
     7381 ccgcaacggc tcgtccgtgg tggcctggcg cgtgccggac cacgccatcg gcggctggcg
     7441 catcgtggcc agccacagcg actcccccac ttggaagatc aagaccgaca tcgttgagaa
     7501 tgacggctgc cgccgcctga gcgtcgaagg ctacggcggg atgatcatgt ccacctggct
     7561 ggaccgcccg ctgaccgtcg gcggccgcgt cattgtcaag accgaggacg gcatcgagag
     7621 ccgccttgtc tgcattgacc gggatctcct cgtcatcccc tcgctggcca tccacttcca
     7681 gcgcgacatc aacaaaggcc acaccttcaa tccgcaggtc gatatgcagc cgctgtgggg
     7741 ccccgcaggc agccgcaccc tgaccgactt ggtagcggag gaactgggcg tggacactgc
     7801 ggacatcctg gacagcgatt tgcagcttgt gacccgccag gccccgaccc agatcggccc
     7861 cgacggtgag ttcttcatgg ccccgcgcat cgatgatctg gaatgcgccg ccacgacgct
     7921 gctgggtttt ctggatgcag ctgccgagac agacagtgcc tgcgccccgg tctgggcgat
     7981 gctcgacaat gaggaagtcg gctcgtcgag ccggatgggc gccgagagca gctacctgcg
     8041 cgacgtactg gaccgcattg ttgacgccat cccccacagc gggcaggcca tgcaccgcgc
     8101 catggcaaac agctttatgc tgagcgccga caacgcccac gccacccatc cgaactttcc
     8161 gcagaagtct gacccctgcg ccccggtacg cctgggcggc ggcgtggtgc tgaagtacaa
     8221 cgccagccag aagtacacga ccaacgccgt cagcggcgcg attttccgcg ccatctgcga
     8281 gaaggccgac gtgccggtgc aagtctttac caaccgcgcc gacgaggccg gcggctcgac
     8341 gctgggcaat ttgcagagcc acacgctgcc catcccgatg gcggatatcg gcctggctca
     8401 gctggcgatg cacagcgccg tggagacagc cagtgtcgcc gacgccgagg ccatgaccaa
     8461 ggccgtggcc gccttctacc gtgtccatct gcgtgccctg ggcgacggca cctacacgct
     8521 ggcatgatta ccggacggta cgcacccagc cccagcggcc ggatgcacct gggcaatttg
     8581 atgtgctgcc tgctggcctg gctgagcgcc aaaagcaagg gcggccaggt gctgctgcgc
     8641 attgaggacc tggacaccca gcgctgcccc cgcgcctacg ccgacgctat catcgacgat
     8701 ttggcctggc tgggcctggc cgccgacggc cccgagccgc caatctatca gagcgaacgc
     8761 agcgccatct accagcagta ttttgacgag cttgcccgcc gggggctggt ttacccctgc
     8821 ttttgcagcc gcagccagct ccacgctgcc agtgcgcccc accgcagcga cggccaggtc
     8881 gtctacgccg ggacctgccg cgggctgacc cccgccgaaa tcgccgcgcg caccaagacc
     8941 cgcgcccccg cctggcgcgt gcaggtgccc gatgaggtca tcgcctttga ggacggccat
     9001 atgggccatt acgcggaaaa ccttgcccgc gactgcggcg acttttacct gcgccgggcc
     9061 gacggtgtgt ttgcctacca gctggccgtc gtcgtggacg atgcactgat gggcgtggac
     9121 gaggtcgtgc gcggcagtga cctcttatcc agcacgccgc gccagctctg gctttaccgg
     9181 gagctggggc tgaccgcgcc gaaattctac catctgccgc tgcttttggc accggacggt
     9241 cgccgtctct caaagcgcga cggcgaccag agcctcgaaa acctgcgcga gaaatacact
     9301 gccggggaaa tcatcggcaa gctggccttt gcctgtaatt tgcagcccaa gcccaccccc
     9361 acgaccccgc aggaattagc ccgcactttt gattgggcta gggtgccaaa aagcgacata
     9421 cttttaccgg aaaagctgtt ttaatataag gtagatacct aaaggcttcc ccccttgggg
     9481 gaagccttct gcgaagcaga ctgatgaggg gcagccttgc cgctgtcgcc ctcttccccg
     9541 ggcggctatg gcaaccgcgc ccctcatccg gcctgcggcc accttccccc aagggggaag
     9601 gcatttacca tttcaccaaa aggagacctt ccccatgcaa cgctatgaca tcaccggcat
     9661 gagctgcgcg gcctgcgcaa gccatgtgga aaaagccgtg gcggctgtgc cgggggttgc
     9721 atccgtcgct gtcagcctgc tgaccaactc catgcaggtc gattacgccc ccgatgccga
     9781 ccctgacgca accgcaaaac gcatcatcaa ggcggtggat gccgccgggt acggcgcatc
     9841 cctcgccacc gccgaaagcg agaacgccga gttggaggac accgagaccc cgaagctgaa
     9901 aaaacgcctg attgccagtc tgtgttttct cgttcccctc atgtatgtct cgatgggtca
     9961 catgatcggc ctgccgctgg gcagcgtgtt ccacggcggc ggttggcacg ccattctgtt
    10021 tgcagtcacc cagctggccc tgaccctgcc cgtctgctgg atcaaccgtg ccttcttcat
    10081 ctcgggctgg aagggcatca agacccgcgc ccccggcatg gacacgctgg tggccctcgg
    10141 cgcgggcgcg tcgctggcct acggcctgtt cgccatcgtc atgatctgcg tcggcctgta
    10201 caccggcaat gacgatttgg tcatgcagta ccgccacgac ctctattttg aatcagcggc
    10261 catgatcctg accctgatca ccgtgggcaa aacgctggaa gcatacagca agggcaagac
    10321 caccgacgcg ctgaaaagcc tgatgaagct ggccccgcag accgcctgtg tgctgcgcga
    10381 cggcgagcag gtcacagtcc cggtcagcga ggtcaatgtc ggcgacctgt tcctggttcg
    10441 ccccggcgag agcatcccgg tggacggcac ggtcgccgag ggcatttcca ctgtgaacga
    10501 ggccgccctg accggcgaga gcatgccggt ggacaagttt cccggctcgc ctgtcagcgc
    10561 cgccaccatc aaccagaacg gtgccctgac ctgccgtgcc gagcgcgtcg gccaggacac
    10621 gacgctgtcc caggtcatcc gcctggtgcg cgacgccgcc gctaccaagg ccccgcttgc
    10681 caagacggcc gataaagttt ccggcatctt cgttcccacc gtcatctgca ttgccaccgc
    10741 aacgttcctg atttggctgc tgctgggcca gaccttcacg ttcgcgctgg cacgcggtat
    10801 cagcgtgctg gtcatcagct gcccctgtgc cctgggcctg gccaccccgg tcgccatcat
    10861 ggtgggcagc ggcgtcggtg ccaagaacgg cattctgttc aagaccgccg ccagcctcga
    10921 aaccaccggc tacaccgaca ctgtgctgct ggataagacc ggtaccgtca ccaccggtga
    10981 gccgagcgtc gtcaaaatcg ttggcaccgg caacgtgccc gaaaagttcc tgctcagcct
    11041 ggccgccggc ctggaactgc gcagcgaaca cccgctggcc cgcgccattt tggaacgtgc
    11101 tgacaaggat aagctctcct acaccaccgt cgccgacttt gaggctgtcc ccggcaaggg
    11161 tttacagggc aaagttgcgg gcaaagtcat cgcgggcggc aacgcaaact tcatccgcga
    11221 gcactgcgag ctgcctgacg atctgaccca ggcgggcgac gccatgagcg ccgacggcat
    11281 cacgccgctg tatttctcgc tggccggcaa ggcggcgggc gtcatcggcg tggccgacgt
    11341 cgtcaagaaa acctcgaaat ctgccatcga ccagatgaag gctctgggcc tgaaggtcgt
    11401 gctgctgacc ggcgacaatg ccgccacggc ccgccacatc ggcgcaacga tcggcctgga
    11461 cgccgaccac gtcattgcag gcgttctgcc tgcgggcaag gaagccgaag tccgcgccct
    11521 gcaggcgcag ggccgcgttg ccatggtcgg cgacggcatc aacgatgccc ccgccttgac
    11581 ccgcgccgag accggcatcg ccatcggtgc cggtgcggac gtggcgctgg acgccgccga
    11641 cgttgtgctg gtgcgcagcg atctggccga cgtgcccgcc gcggtgcgcc tgtcccgcgc
    11701 ggtggtgcgc aacatccacc agaacctgtt ctgggcgttc ttctacaacg ccatctgcat
    11761 tccgctggcg gcgggcgtgt tcaccggtat cggcatcacg ctgaacccga tgatcgcctc
    11821 ggcggccatg agcctttcca gcgtctgcgt tgtcaccaac gcgctgcggc tcaacgcctt
    11881 tgacccgcgc aatgccgccc acgacgcccc gccgaagcgc aaggccccgg cccgcaaacc
    11941 catttctgaa cccaccacct gccccaccgg cagctgccct gtgcagcctg ccgaaatcaa
    12001 cgataaaacg gaggaaatca ccatgaacaa gacgattcac attgaaggca tgatgtgcgg
    12061 ccactgcgag gccgctgtga agaaggccct ggaagccctg gacggcgttg ccggcgccac
    12121 ggtcagccac gagcagggca cggccatcgt cgccctgacc catgacgtgg ccgacgccga
    12181 gctgaaggcc gccgtcgagg ccaaggacta caccgttacc ggcatcgacg catgagcacc
    12241 cttcgccgcc gctggcggtt caccggcacg gtgcagggag tgggctttcg ctattacgcc
    12301 cgcgccgccg cactgcacct ggggctgacc ggctgggtcg ccaacaactg ggacggcagt
    12361 gtcacgctgg aagcccaggg cgaccgcgcc gcgctggacg cgctggtccc cttgatcgag
    12421 cgcaccaacc gctgggcccg cattgagaac atcgagaccg ccatcctccc cctcaaggag
    12481 catgagtacg ggtttgagga acggagcggg gaataaattt acgggcagcg ctgccgaatg
    12541 gcagcgctgc ccgctttttt gcttgcactt acccgctgtt acggctataa taatattatg
    12601 gcaataccgc acaattcccg cctgacggct taagcaccgc tccggcggct acggcacggc
    12661 atctgcgttg ccaaaatgct cgataagacc tatatcaata tcaaaagggg ttttcgtttt
    12721 gcccggaact ttatatcttg ttgctacgcc tatcggcaat ctggacgata tgccgccccg
    12781 cgtggctgcc accttcggca tggcggattt catcgccgcc gaggataccc gcgtgaccct
    12841 gcggctgctg aaccatctgg ggctgaaaaa gccgatggtc agctactatg aacacgcgct
    12901 ccaccacggc gaggccattt tgcagcgcat tgaagcgggc gaaaactgcg ccctgtgcag
    12961 cgatgccgga atgccctgca tcagcgaccc cg
//
LOCUS       DBMDSQ010000031        12857 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_1491_length_12857_cov_1.801192, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000031 DBMDSQ010000000
VERSION     DBMDSQ010000031.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 12857)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 12857)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12857
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1491_length_12857_cov_1.801192"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..300
                     /locus_tag="ACZPAX_02170"
     CDS             <1..300
                     /locus_tag="ACZPAX_02170"
                     /inference="COORDINATES: protein motif:HMM:NF013540.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR1112736.1"
                     /translation="GRDSLISDNIKRLRENMGLSQAALARKLGVTRASVNAWEMELSS
                     PTAVYLVEMAQLFHTTTDDILGLESEEKISLRGMNEQEKRLVYDLVDYISGHHKP"
     gene            complement(423..1451)
                     /gene="gap"
                     /locus_tag="ACZPAX_02175"
     CDS             complement(423..1451)
                     /gene="gap"
                     /locus_tag="ACZPAX_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015518656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HIR1112737.1"
                     /translation="MAVKVAINGFGRIGRLAFRQMFEADGYEVVAINDLTSPAMLAHL
                     LKYDTAQGSFLGKIGENKHTVEAGEDYIVVDGKKITIYAIKDAKDCPWGELGIDVVLE
                     CTGFYTSKAKAEAHIQAGARKVVISAPAGNDLKTIVYSVNEKTLTADDQVISAASCTT
                     NCLAPMADTLNKAFPIVSGIMTTVHAYTGDQMILDGPQRKGDLRRARAGAQNIVPNST
                     GAAKAIGLVIPELNGKLIGSAQRVPVPTGSTTILVAVVKGKDVTKEAINAAMKAASSE
                     SFGYNEDPIVSSDVIGMRYGSLFDATQTMVAKIDDDTYQVQVVSWYDNENSYTSQMVR
                     TIKYFAEL"
     gene            1688..2266
                     /locus_tag="ACZPAX_02180"
     CDS             1688..2266
                     /locus_tag="ACZPAX_02180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1112738.1"
                     /translation="MPFFGPTRAALAAAAARGADIIPSLRLVLTAEALTAPPPAHALE
                     LNAELDALCAAARELAVCRAGWLYFCGTDAPLPAAVPRGLLQGTVLTFLRGVLRSEGR
                     AVVRLLPQGDSAVLALQGGSPARMPGDLPALLHRCGPYVTAAGARYAATVRLALSPTL
                     PLTPLSDADTLVLDRYSPPRVYLQGFCVEDVE"
     gene            complement(2373..3197)
                     /locus_tag="ACZPAX_02185"
     CDS             complement(2373..3197)
                     /locus_tag="ACZPAX_02185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1112739.1"
                     /translation="MKNKHPNAIVVLALGFAMAGLHLAGLLLWSDAETGFATAGSVWL
                     RYIPWLAALVLPYLPARRAANQPTGLQDTCPPLGLCMVLAGAALVTSGVAMGPVAKYA
                     VQYPEMFSTDYPTWAVWADLLLPVLCGAWLLYYGGRAFKGFGMQRQKLGSPLFAGTVP
                     LYFLWKLIWRFQFTPASVYRMPCALRVLSAAAALLFAVVLIKVFLVPGLPCGHTLYAA
                     GTGAYLLCTGLELTQTLFEAAHNMLTLPDLAAGLGIGLFGLCGLFCAWEACGKEME"
     gene            3310..4320
                     /locus_tag="ACZPAX_02190"
     CDS             3310..4320
                     /locus_tag="ACZPAX_02190"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030435 - sporulation resulting in
                     formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HIR1112740.1"
                     /translation="MKKTFRRAVVFGLLALVAPFAALPLAKPATTDLQSTTPPPEVLP
                     EPAATPLPVYTAAPMQRSFNASAPILICDDGENTSVSVQDFLIGTAACELPPDWPDDA
                     ILAQMVAAHSYALSLGGAAFSCNSAQCAGWTTAEVLRARWNGEFSTYYSRLAALADEA
                     AGAVLCWEGSPAAACYHSISAGQTEASQNVWLTAVPYLQGVSSPWDADAPGFETAVTY
                     SAEQVYSVLLALGLDADEIPNAPADWFGGGVSDAAGYVAQMPLCGQVFTGTKLRNAFS
                     LRSAAFTVAYDPGENAFVFTTHGYGHGVGLSQYGAKAMAEDGKDWREILEWYFPGCEV
                     VE"
     gene            complement(4466..5635)
                     /locus_tag="ACZPAX_02195"
     CDS             complement(4466..5635)
                     /locus_tag="ACZPAX_02195"
                     /inference="COORDINATES: protein motif:HMM:NF025159.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4179 domain-containing protein"
                     /protein_id="HIR1112741.1"
                     /translation="MRNKNFDADLLKTLNEQPMPAAPLTDDEFARVLDAACAKLPQAK
                     KTVKRRFPWGRVAAAAAACVAVGLGSVNFAAPAVAEQLPVVGSLFSWLNRGGQEDYTS
                     LHSEQLNKYAETVETTAESAGSPYTLTLGQVFNDGDWLRISLMLTAEDDSLAGFNAIG
                     PSEDAVEKALQNGGGQYGTLVLDNGTELNGGVTFEKRDDRTFVTGINYELFLAPDDLA
                     GHTATLTLSDLVACNKSILETDGQSYWHYDYADQTPLPGTYTLTFTIPEVSDAGVRTM
                     DKPMEQNGITLQSVTATPAATKVLLSFPAEQHWVNVRLFTEDGTELGHERGEGGWWTD
                     GAWTADPADFDHPEQCTKASYDYFAAVPENCRSLTVKVYDFDTDAELTTFTVELP"
     gene            complement(5628..6182)
                     /locus_tag="ACZPAX_02200"
     CDS             complement(5628..6182)
                     /locus_tag="ACZPAX_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR1112742.1"
                     /translation="MKDRELTRLLQTQPEEGLEAAMLDYAPLVKAVLNRILPQNPCDR
                     EECMADVFVSLWRNAAKLERTATPLRPWLIVTARNKGIDRYNALRRSETVTLDDGLAE
                     TLGELAEFDRATADAADLVGALVAAMEPPDRDIFLRKYYLLQSGKEIAAALDMSVESV
                     NTRLSRGRDRLRRQLTEKGVYTHA"
     gene            complement(6463..>6659)
                     /locus_tag="ACZPAX_02205"
     CDS             complement(6463..>6659)
                     /locus_tag="ACZPAX_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005934700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR1112743.1"
                     /translation="APEALPFVFERFYKEDRSRGMNTRGSGLGLHICKILINLSGGQI
                     HAESEEGKWCRFVFTLPAGQ"
     assembly_gap    6660..6669
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6670..7907)
                     /locus_tag="ACZPAX_02210"
     CDS             complement(<6670..7907)
                     /locus_tag="ACZPAX_02210"
                     /inference="COORDINATES: protein motif:HMM:NF012722.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HIR1112744.1"
                     /translation="MSRRNTISREFFATIAAVLVLGLSVMCAIQTALSAAYFISERKS
                     SLTDVLNGAAALSERFADEGSIVTKPLQDDDIMERAQSGFELFNTASGALIFIADGNG
                     NILLHTGNDAFTGGSVPTEYIDDLNEGRDIFETGTLDGVYNAKYYTAGRRITVGGQDG
                     YLFAASPMAALGGYMSDMLAMFGISAAVILILCSILCWVLARRITGPIEDISEAARRL
                     GSGDFTARAPVDGCVELADFATTFNNMAARLQTIDNSRGQFMGNIAHELRTPMTTIKG
                     FIDGMLDGTIPQEETKHYLGIVSQETGRLARLVQNMLDITKLEAGEVPIHAQTYDLWK
                     TVTDVVLSDEQRIEDGKIDIQGLGGDPLPIYADPDFVHQVVYNIVDNAIKFTPAGGTI
                     SFAAERHGNMVEMRIENTGAG"
     gene            complement(7904..8593)
                     /locus_tag="ACZPAX_02215"
     CDS             complement(7904..8593)
                     /locus_tag="ACZPAX_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015564480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR1112745.1"
                     /translation="MANEKILVVDDDKNICELLRLYLVKEGYNVTMVHDGAAALAEFD
                     KLHPDLVLLDVMMPVMDGWEVCRKIRAKDNTPIIMLTAKGETYDKVLGLELGADDYIV
                     KPFDAKEVTARIKAVLRRSSAKEEENKGVYDFDNLHLDMNRYELKVKGKVVEAPPKEL
                     ELLACLAGHPNRVYTRDQLLDEVWGFEYYGDSRTIDVHVKRLREKLEGASEQWSLKTV
                     WGVGYKFEVKE"
     gene            8899..10029
                     /gene="ychF"
                     /locus_tag="ACZPAX_02220"
     CDS             8899..10029
                     /gene="ychF"
                     /locus_tag="ACZPAX_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005921447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HIR1112746.1"
                     /translation="MKLGIVGLPNVGKSTLFNALTSTQNAQAANYPFCTIEPNSGIVP
                     VPDPRLDKLAEIWQTDKKTPAIVEFVDIAGLVKGASQGAGLGNKFLGHIRECDAIVHV
                     VRCFDDDNIIHVVEDVNKPESVDPIRDIDAIDLELILADLEVVSNRLGRQQKAAKTGN
                     KAAAAEAAWLADLAAWLEGGKPARSFAFDESDDAVKATRKELGLLSAKPVIYACNVGE
                     DDLLGGLDENPYYPLVAARAKEENAQAIPICAKTEEDIAGSTQEEKIAFLQEMGIEST
                     GLDKLIKASYELLGLISFLTDGKKECRAWTIKRGTKAPQAAGKIHSDFERGFIRAQVI
                     AYKDLEDADFNYAAVKAKGLQRTEGKEYVVNDGDVIEFLFNV"
     gene            10043..10726
                     /locus_tag="ACZPAX_02225"
     CDS             10043..10726
                     /locus_tag="ACZPAX_02225"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005921445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HIR1112747.1"
                     /translation="MIIGIMGAMPDEVDQLCARLENVTRETYAGVEYHCGTLQGKQVV
                     VCCAGMGKANAASTVQVLCTKYGIDKLIFSGIAGNMTSKIGIGDVCIGETVVYHDAEL
                     SMIAQSAPFQTEYHGDPALVRAALDACAACGVKAIAGKIATGDTFVGDAETKKAIEEK
                     CHPDCVEMEGAAVSQVAGRNGVPCVILRAMSDNADESGYEVLVVKQFSITEYVKTATE
                     IVARMIESV"
     gene            complement(10907..12094)
                     /locus_tag="ACZPAX_02230"
     CDS             complement(10907..12094)
                     /locus_tag="ACZPAX_02230"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015564688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIR1112748.1"
                     /translation="MQFTELTNVSPEIIRATQAMGFTDMTEIQEKAIPLMLGGHDMIA
                     KAPTGTGKTVAFGIPILSRVDPGSLKPQAVILSPTRELAQQIAQDLQNLAQFLPAIKI
                     VCVYGGAGMDKQQRQLKQGCQIVVATPGRLMDHYRHHAIDVSDVKTVVLDEADEMLNM
                     GFYKDVKYIIDLMKHRESLSMFSATISREVLDIGWLYQHNAAEVSVQPVEDSSPKIAQ
                     YKLLTTGRDKLADAAQIIIDEGYKRVMIFCNTKYNTGMLANQLARLNFNVDCLHGDLS
                     QAERNRIMTRFKAGEIDVLVATDVAARGIDVSDVDAVINYDVPEENEHYTHRIGRTGR
                     ARKQGASYLFYTKEEQKRVDQLLRLTRNTDDCKAVKFDFNHEHLKVEEARQEDKFQIK
                     AYF"
     gene            complement(12190..12774)
                     /locus_tag="ACZPAX_02235"
     CDS             complement(12190..12774)
                     /locus_tag="ACZPAX_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RICIN domain-containing protein"
                     /protein_id="HIR1112749.1"
                     /translation="MKKSAQTVIRPDACYTIAAANGRVLEVADFNTENGASVRLWSYE
                     GQPWQQWFFVEAGENEYRIKNRFTGKVMDLALSGVENGTWVHQWSATSGAGQRWQIVD
                     AGGGKVKLRNVLADKVIDLVGMRVENGTQAQIWQDVYGENQLWKLDPVPARLLDKEPP
                     APKPGTRKAAPKTTRTQTEKKTSGKAARRTSKNK"
BASE COUNT         2423 a         3915 c         3914 g         2595 t
ORIGIN      
        1 ggacgtgata gcttgatttc tgacaacatc aaaaggttgc gggaaaacat ggggctctcg
       61 caggcggcgc tggcccgcaa gctgggggta acgcgcgcca gcgtcaatgc gtgggaaatg
      121 gagttatcct cccccacagc agtctacctt gtggaaatgg cacagctgtt ccacacaact
      181 accgatgaca tcctcggcct ggaaagcgaa gaaaaaatca gcctgcgcgg tatgaacgaa
      241 caggaaaagc gcctcgtcta cgacttggtc gactatatca gcgggcacca caaaccataa
      301 ccccgccccg ggcgcacaca caggtgcgcc cttacatccg ctaataacaa aaaccgcccc
      361 ttccggtttg acccggaagg ggcggtttca gctattcaat tcgcagttaa gctactcgca
      421 gattacagct cagcgaagta cttaatggtg cggaccatct gagaggtgta ggagttctcg
      481 ttgtcgtacc aagaaacgac ctgaacctga taggtgtcgt cgtcgatctt agcgaccatg
      541 gtctgggtag catcgaacag ggagccatag cgcataccga taacatcgga agagacgatc
      601 ggatcctcgt tgtagccgaa gctctcagaa gaagcagcct tcatagcggc gttgatagcc
      661 tccttggtga cgtccttgcc cttgacaacg gcaaccagaa tggtggtgga gccggtgggg
      721 acgggaacgc gctgagcaga gccaatcagc ttgccgttca gctcggggat gaccaggccg
      781 atggccttgg cagcgccggt ggagttagga acgatgttct gagcaccagc acgagcgcgg
      841 cgcaggtcgc ccttgcgctg cgggccgtcc aggatcatct ggtcgccggt gtaagcatga
      901 acggtggtca tgatgccgga gacgatgggg aaggccttgt tcagggtgtc ggccatgggg
      961 gccaggcagt tggtggtgca ggaagcagcg gagatgacct gatcgtcagc ggtcagggtc
     1021 ttctcgttga cggagtagac gatggtcttc aggtcgttgc cggcgggagc agagataacg
     1081 accttgcggg cgccagcctg gatgtgagcc tcagccttag ccttgctggt gtagaagccg
     1141 gtgcactcca gaacgacgtc gatgcccagc tcgccccagg ggcaatcctt agcatcctta
     1201 attgcgtaga tggtgatctt cttgccgtcg acgacgatgt aatcctcgcc agcctcaacg
     1261 gtgtgcttgt tctcgccgat cttgcccagg aaagaaccct gtgcagtgtc gtacttcagc
     1321 aggtgagcca gcatagcggg gctggtcaga tcgttgatgg cgacaacttc gtagccgtca
     1381 gcctcaaaca tctgacggaa agccagacgg ccgatacggc caaaaccatt gatagcaact
     1441 ttaacagcca tagtgaaatc ctcccaagat ttgtcttttt caccttatga gcggtttccc
     1501 gctcgtcggt atgatactat tgtaccctat ttttcacaat tctgcaaggg ggtctgttat
     1561 tcttttaaca cgatttttca aaaaatccac agaaattttg aaatcccagc catttccagc
     1621 ccggtttggg ggtcggttgg ggcagactaa gggtaaattt cgcaccggat ggggagaggt
     1681 gagtgctatg ccatttttcg gcccgacgcg ggcggcgctg gcggcggctg cggcccgcgg
     1741 ggcggatatt ataccctcgc tgcggctggt gttgactgcc gaggccctga ctgcgccccc
     1801 gcccgcccat gcgctggagc tgaacgcaga attggacgcg ctgtgtgccg ccgcgcggga
     1861 gctggccgtc tgccgcgccg ggtggctgta tttctgcggc acagatgccc cgctgcccgc
     1921 cgccgtgccc cgcgggcttt tgcagggcac ggtgctgacg tttttgcgcg gggtgctgcg
     1981 cagcgagggc cgggccgtgg tgcggctgct gccccagggt gacagcgccg tgctggcctt
     2041 gcagggcggc agccccgcgc ggatgcccgg cgacctgccc gcgcttttgc accgctgcgg
     2101 gccgtatgtg accgccgccg gggcgcggta cgccgcaacg gtgcggctgg cactctcccc
     2161 caccctgccg ctgacgccgc tgtccgatgc ggatacactg gtattggacc gatacagccc
     2221 gccccgggtg tatttacagg ggttttgtgt ggaggatgtg gaatagcggc cgcgcaaaaa
     2281 gcccctcagg cctcgcaggc tcgaccggcc tccccttaca ggggaggtag ccgagcgcca
     2341 gcgatggtcg gaggggcttt gcagcttttc ctttattcca tttctttccc gcaggcttcc
     2401 cacgcacaaa acagtccgca caggccaaac aacccaatac ccaatcctgc cgccaagtcg
     2461 ggcagggtca gcatattgtg ggctgcttca aacagggtct gggtcagttc cagcccggtg
     2521 cagagcagat atgccccggt gcccgccgcg tacagcgtgt ggccgcaggg caaccccggc
     2581 acaaggaaca ctttgatcaa caccacggca aacagcagcg ctgctgccgc gctgagcacc
     2641 cgcagtgcgc agggcatacg gtagaccgac gcgggcgtga actggaaccg ccaaatcaat
     2701 ttccacaaaa aatagagcgg caccgtgccg gcaaacagcg ggctgcccag cttttgccgc
     2761 tgcatcccaa aacccttgaa cgcccggccg ccgtaataca gcagccatgc gccgcacaaa
     2821 accggcagca acaaatcggc ccagaccgcc caggtcgggt agtcggtgga gaacatttcc
     2881 gggtactgca ccgcgtattt tgccaccggc cccatggcca cgccgcttgt gaccagcgcc
     2941 gccccggcca gcaccataca cagccccaac ggcgggcagg tgtcctgcaa accggtcggc
     3001 tggtttgccg cacggcgggc gggcagatag ggcagcacca gcgccgccag ccaggggatg
     3061 taccgcagcc agaccgagcc cgccgtggca aagccggttt cggcgtcgga ccagagcagc
     3121 aggccggcca ggtgcagccc cgccatagca aaacccaggg ccagcaccac aatggcgttg
     3181 ggatgtttat ttttcatgaa agttcctcat ttcatacagg gcgcggccgc cgcatatata
     3241 taggacaagc cgcgcgggat attcctattg taccgccccg cgccgcaaat tgcaaggagg
     3301 gcacgcccct tgaaaaaaac atttcgccgg gccgtagtgt tcggcctgct ggcccttgtc
     3361 gcgccctttg ccgccctgcc gctggccaag cccgccacca ccgatttaca gagtaccacc
     3421 ccgccgcccg aggttctgcc cgaacctgcc gccacgccgc tgccagtgta caccgccgcc
     3481 cccatgcagc gcagctttaa cgcatctgcg cccattttga tttgtgacga tggggagaac
     3541 acttcggttt ccgtgcagga ttttctcatc ggcacagccg cctgtgagct gccgcccgac
     3601 tggcccgacg acgcgatttt agcccagatg gtggccgccc acagctacgc gctgagcctg
     3661 ggcggcgcgg cgttcagctg caacagcgcc cagtgcgcgg gctggacgac ggccgaggtg
     3721 ctgcgggcgc ggtggaacgg cgaattttct acgtattata gtaggctggc cgcactggca
     3781 gacgaagccg ccggggcagt gctctgctgg gagggctccc ccgccgctgc gtgctaccac
     3841 agcatcagcg ccgggcagac cgaggcaagc caaaacgtct ggctgaccgc cgtgccgtac
     3901 ttacaggggg tgtcgtcgcc ctgggacgcc gacgcgccgg ggtttgagac tgccgtcacg
     3961 tacagtgccg agcaggtgta cagcgtcctg ctggcgctgg ggctggatgc cgacgagata
     4021 ccaaacgccc ccgccgattg gttcggcggg ggcgtatcgg acgcagcggg ctatgtggcg
     4081 cagatgccgc tctgcgggca ggtgttcacc gggacaaagc tgcgcaacgc gttcagcctg
     4141 cgatccgcgg cgttcacggt ggcgtatgat cccggggaga atgcgtttgt ttttacgacc
     4201 cacggctacg gccacggcgt cgggttgagc cagtacggtg caaaggccat ggccgaggac
     4261 ggcaaggatt ggcgggagat tttggagtgg tactttccgg ggtgtgaggt ggtggagtaa
     4321 ccccctgcct tcccccttgg gtgaaggtgg ccccgcaggg ccggatgagg ggcgtgcgtg
     4381 tcgtcattgc cccacaggac tcaatcgggc aaggccgccc ctcatcagtc cgcttcgcgg
     4441 acagtttccc ccgaggggga agcctttacg gcagctccac ggtaaaggtc gtcagctctg
     4501 catcggtgtc aaaatcgtag acctttaccg tcaggctgcg gcagttttcc ggcacggcag
     4561 caaagtagtc atagctggcc ttggtgcatt gttccgggtg gtcgaagtcc gccgggtcgg
     4621 ccgtccatgc gccgtccgtc caccagccgc cctcgccgcg ctcgtgcccc agctccgtgc
     4681 catcctcggt gaacagcctc acattcaccc aatgctgttc cgccgggaag ctcagcagca
     4741 ctttcgtcgc ggcgggcgtg gccgtgacgc tttgcagggt gatgccgttt tgttccatgg
     4801 gtttatccat cgtgcgcacg cccgcgtcgc tgacttccgg gatcgtaaac gtcagcgtgt
     4861 aggtgccggg cagcggggtc tggtctgcgt agtcatagtg ccaataactc tggccatcgg
     4921 tttcgagaat actcttgttg caggccacga ggtcggacag cgtcagcgtt gccgtgtgcc
     4981 cggccaaatc atcgggtgca aggaacagct cataattgat gcccgtcaca aaggtgcggt
     5041 catcgcgttt ttcaaaggta acaccgccgt tcaattccgt gccgttgtcc agcaccagcg
     5101 tgccgtactg cccgccgccg ttctgcagcg ccttttcaac ggcatcctcg ctggggccga
     5161 tggcgttgaa cccggccaga ctatcgtcct cggcggtcag catcaggctg atgcgcagcc
     5221 agtcgccatc gttaaacacc tgccccagtg tcagggtgta ggggctgccg gcgctctctg
     5281 cggtggtttc caccgtctcg gcgtatttgt tcagctgctc gctgtgcagc gaggtgtagt
     5341 cctcctgtcc gccgcggttc agccagctga acaggctgcc caccacaggc agctgttcgg
     5401 ccacggcggg ggcggcaaag ttgacactgc ccagccccac ggccacgcag gctgcggccg
     5461 ctgcggctac ccgcccccac gggaaacggc gcttgacagt cttttttgcc tgcggcagct
     5521 tggcacaggc cgcgtccagc acgcgggcaa attcatcatc ggtcagcggg gcggcgggca
     5581 tcggctgctc attcagcgtt ttcaacaaat cggcatcaaa atttttgtta cgcatgggtg
     5641 tacactccct tctcggtcag ttggcggcgc aggcggtcac gcccgcggct caggcgggtg
     5701 ttgacgctct caacactcat atctagcgcg gcggcgatct ccttgccgga ttgcagcaga
     5761 taatacttgc gcagaaagat gtcccggtcc ggcggttcca tggcggctac cagcgccccg
     5821 acaaggtcgg cggcatcggc ggtggcgcgg tcaaactcag ccagctcgcc gagcgtttcg
     5881 gccaggccgt cgtccagcgt caccgtctcg ctgcggcgca gggcgttgta gcggtcgatg
     5941 cccttgttgc gcgccgtgac aatgagccac ggccgcagcg gcgtggcggt gcgctccagc
     6001 ttggccgcat tgcgccagag ggagacgaac acgtcggcca tacattcctc ccggtcgcag
     6061 ggattctgcg gcaggatgcg gttcagcacc gcctttacca gcggcgcgta gtcgagcatg
     6121 gctgcctcca gaccctcctc cggctgtgtc tgcagcaatc gggtcaattc ccggtctttc
     6181 atgttggtac ctcctttttg tgggttctga ctatatagac ccgggcggga ggagaaatct
     6241 tacagggttt ccaaaaaagc cttcccactt tgggggaagg tggccccgca gggccggatg
     6301 aggggcgagt ccgccgcgat tgcccgttcc cgggttgccg tggcacacct gcccctcatc
     6361 agtcagcggg cggggccgct gacagcttcc cccgaggggg aagcctttct cacgcacaaa
     6421 aaagcagacg ccgcttttgt gcagcgtctg ttatgcggtt gcttattgcc ctgccggcag
     6481 ggtgaacacg aaccggcacc atttgccttc ctcactctca gcgtggatct ggccgccgga
     6541 caggtttatc aaaatcttgc agatgtgcag tcccaggccg ctgccgcggg tgttcatgcc
     6601 gcgggagcga tcctccttgt agaaccgctc aaacacaaag ggcagggcct ccggcgcaan
     6661 nnnnnnnnnc ccgcgccggt attctcaata cgcatctcca ccatgttgcc gtggcgctcg
     6721 gcggcaaagc tgatggtgcc gcccgccggg gtgaacttga tggcattgtc tacgatgtta
     6781 tacacgacct gatgcacaaa atccgggtcg gcatagatcg gcagcgggtc gccgcccagc
     6841 ccctgtatgt cgattttgcc gtcctcaatg cgctgctcgt cggacagcac cacgtcggtg
     6901 acagttttcc acagatcgta agtctgggcg tggataggca cctcgcccgc ctcgagcttg
     6961 gtaatgtcca gcatattctg caccagccgc gccaggcggc ccgtctcctg cgagacgatc
     7021 cccaggtaat gcttggtttc ttcctgcgga atcgtgccgt ccaacatgcc gtcgatgaag
     7081 cccttgatgg tcgtcatcgg ggtacgcagc tcgtgagcaa tgttgcccat gaactgcccg
     7141 cgggaattgt cgatggtttg cagccgcgcg gccatattat taaaagtagt cgcaaagtcg
     7201 gccagctcca cacaaccgtc caccggggcg cgggccgtga agtcgccgct gcccagccgc
     7261 cgcgcagcct cgctgatatc ctcgatcggg ccggtgatgc ggcgggccag cacccaacac
     7321 aggatgctgc acaaaatcag aatgaccgct gccgagatgc cgaacatcgc cagcatatcg
     7381 ctcatgtagc cgcccagcgc tgccatgggg ctggcggcaa acaggtagcc gtcctgcccg
     7441 cccactgtga tgcgccgccc ggcggtgtag tattttgcgt tgtacacgcc gtccagcgtg
     7501 ccggtctcaa agatgtcgcg gccctcgttc aggtcgtcta tgtactcggt tggcacgctg
     7561 ccgccggtga aagcgtcgtt gccggtgtgc agtaaaatgt tgccgttgcc gtccgcaatg
     7621 aaaatcagcg ccccggatgc cgtgttgaac agttcaaacc cactttgggc gcgctccata
     7681 atatcgtcat cctgcaaagg ctttgtgacg atggagcctt cgtcggcaaa ccgctcactc
     7741 aatgcggccg cgccattgag cacatcggtc agcgaacttt tgcgctcgct gatgaagtac
     7801 gccgccgaga gtgctgtctg gatggcgcac atcacgctca gccccagcac aagcacggcg
     7861 gcgatggtgg caaaaaattc gcggctgatc gtgttgcggc ggctcattcc ttgacctcga
     7921 acttatagcc gacgccccag acggttttca ggctccactg ctcggacgca ccttccagct
     7981 tctcgcgcag acgcttgaca tggacgtcga tggtgcggga gtcgccgtaa tactcaaagc
     8041 cccacacctc gtccagcagc tggtcgcggg tgtacacgcg gttggggtgg cccgccaggc
     8101 aggccagcag ttccagctcc ttgggcgggg cctcgacgac cttgcccttg actttcagct
     8161 cgtagcggtt catatcgagg tgcaggttgt cgaagtcgta gacgcccttg ttttcttcct
     8221 ccttggcgga ggagcggcgc agcacggcct tgatgcgggc cgtgacctct tttgcgtcaa
     8281 agggcttgac gatatagtca tcagcgccca gttccaggcc cagcaccttg tcatacgtct
     8341 cgcctttggc ggtcagcatg atgatggggg tgttgtcctt ggcacggatt ttacggcaga
     8401 cctcccagcc gtccatcacc ggcatcatga cgtccagcag caccagatcg gggtgcagct
     8461 tgtcgaactc ggccagggcg gccgcgccgt catggaccat cgttacgttg tagccttcct
     8521 tgaccaggta caggcggagc agttcgcaga tatttttatc gtcatcgacg accagaattt
     8581 tctcatttgc catcttggtt tcctccctaa gccgccaggc ggcagtttat aagattatta
     8641 taccgcataa ttgtggacaa aaaaagaaca gatgaccgtc tttgtgcaaa tttttcgtga
     8701 cagaacaaat ttatggtatg cccccctgcc gggcgttctc aatagtgatt tggctgcgcc
     8761 gccagaaatc gccaaaagcc gccgcaagcc cgttgaaagc ggtgcatttc tgtgttataa
     8821 tttaaatagt atgtcatgat tcaggggcaa caccccccgc gataaaaagc aacgacaaca
     8881 aaggagatat agaacatcat gaaattgggc atcgtcggcc ttccaaatgt gggcaaatcc
     8941 acccttttca acgcgctgac cagcacgcag aacgcgcagg cagccaacta tcctttctgc
     9001 accattgagc cgaactccgg catcgtgccg gtgccggatc cgcggctgga caagctggcc
     9061 gagatctggc agaccgataa aaagacgcct gccatcgttg agtttgtcga cattgcaggt
     9121 ctggtcaagg gtgccagcca gggcgcgggc ctgggcaaca agttcctggg ccacatccgc
     9181 gagtgtgacg ccatcgtcca tgtcgtgcgc tgctttgatg atgacaacat catccacgtc
     9241 gtcgaggacg tcaacaagcc cgagagcgtt gaccccatcc gcgacattga tgccatcgac
     9301 ctggaactga ttctggccga cctggaagtc gtcagcaacc gcctgggccg ccagcagaaa
     9361 gccgccaaga ccggcaacaa ggccgccgca gctgaggccg cctggctggc cgacctggcc
     9421 gcctggcttg agggcggcaa gcccgcccgc agctttgcct ttgacgagag cgacgatgcc
     9481 gtgaaggcca cccgcaagga gctgggcctg ctctccgcaa agccggtcat ctacgcctgc
     9541 aatgtgggtg aggacgacct gctgggcggc ctcgacgaga acccctacta tcctctggtc
     9601 gccgcccgcg ccaaggagga aaacgcccag gctatcccca tctgcgccaa gaccgaggag
     9661 gacattgccg gttccacgca ggaggaaaaa atcgcgttcc tgcaggaaat gggcatcgag
     9721 tccacgggcc tcgacaagct catcaaggcc agctatgagc tgctgggcct catcagcttt
     9781 ttgacggacg gcaaaaagga gtgccgcgcc tggaccatca agcgcggcac caaggccccg
     9841 caggctgccg gtaagattca cagcgacttc gagcgcggct tcatccgcgc ccaagtcatt
     9901 gcctacaagg atctggagga cgccgacttc aactacgccg ccgtcaaggc caagggcctg
     9961 cagcgcaccg agggcaagga atacgtcgtg aacgacggcg atgtcatcga gtttttgttt
    10021 aatgtataag gagaatccga ccatgattat cggcattatg ggcgccatgc ccgacgaagt
    10081 ggaccaactg tgtgcccggc tggagaatgt gaccagggag acctacgccg gggtggagta
    10141 ccactgcggc accttgcagg gcaagcaggt cgttgtctgc tgcgcaggca tgggcaaggc
    10201 caacgccgcg tccaccgtgc aggtgctgtg cacgaaatat ggcattgaca agttgatttt
    10261 tagcggcatt gcgggcaaca tgaccagcaa aatcggcatc ggcgatgtct gcatcggtga
    10321 gacggtcgtc taccacgacg ccgagctgag tatgattgcc cagagtgcac cgttccagac
    10381 cgagtaccac ggcgaccctg cgctggtcag ggccgcgctg gacgcctgtg ctgcctgcgg
    10441 cgtgaaggct attgcgggca agatcgccac cggcgacacc ttcgtcggcg acgccgagac
    10501 gaaaaaagcc atcgaggaaa agtgtcatcc cgactgtgtc gagatggagg gcgcggcggt
    10561 cagccaggtg gcgggccgca acggcgtgcc ctgcgtgatt ctgcgtgcca tgagcgacaa
    10621 cgccgacgag agcggctatg aggtgctggt ggtcaagcag ttcagcatca ccgagtacgt
    10681 taagacggca acggaaatcg tcgcgcggat gatcgagagc gtgtaaggtt ctacaaaaag
    10741 gcacctgcct gtgtggaata ggcaggtgcg ttttttgttg taggtagcgg cgtcctcgac
    10801 gccccgcgga agggcaaggc ccctctctct acaatgccaa agataaaggg gctatccccc
    10861 tcacgttgcg ggccgggtat gcccgatccc tacggctttt ctttcctcaa aaatacgcct
    10921 tgatctggaa cttatcctcc tgcctggcct cttccacctt cagatgctcg tggttgaagt
    10981 caaacttcac ggccttgcag tcgtcggtgt tgcgggtcag gcgcagcagc tgatccacac
    11041 gcttctgctc ctctttcgtg tagaacaggt agcttgcgcc ctgcttgcgg gcgcggcccg
    11101 tgcggccaat gcggtgggtg tagtgctcgt tttcctcggg cacatcgtag ttgatgaccg
    11161 catccacgtc ggaaacgtcg atgccgcggg cggccacatc ggtggcgacc agcacatcga
    11221 tttccccggc cttgaaccgc gtcatgatgc ggttgcgctc ggcctgggac aaatcgccgt
    11281 gcagacagtc cacattgaaa ttcagccggg ccagctggtt ggccagcata ccggtgttgt
    11341 acttggtgtt gcagaagatc atgacccgct tgtagccctc gtcaatgatg atctgggcgg
    11401 cgtcggccag cttgtcgcgg ccggtggtca gcagcttgta ctgggcaatt ttcgggctgg
    11461 aatcctccac cggctgcacg ctgacctcag cggcgttgtg ctggtagagc cagccaatgt
    11521 ccagcacctc acggctgatg gtggccgaga acatcgacag gctctcgcgg tgcttcatca
    11581 ggtcgatgat gtacttgaca tccttgtaga agcccatgtt cagcatctcg tcggcctcgt
    11641 ccagcacgac ggtcttgacg tcggacacat caatggcgtg gtggcggtag tgatccatca
    11701 ggcggcccgg cgtggcaaca acgatctggc agccctgttt gagctgacgc tgctgcttgt
    11761 ccatgcccgc gccgccgtag acgcagacga ttttgatggc aggcagaaac tgcgccaggt
    11821 tttgcaggtc ctgggcgatc tgctgggcca gctcacgcgt ggggcttagg atgacggcct
    11881 gcggcttgag gctgccgggg tcaaccctgg acaggatggg gataccgaac gccacggttt
    11941 tgccggtccc cgtgggggcc ttggcaatca tatcgtgccc gcccagcatc agcggaatgg
    12001 ccttttcctg aatttcggtc atatcggtaa agcccatcgc ctgcgtagcg cggatgatct
    12061 cgggcgaaac attggtaagt tcggtaaact gcatggttgg tactgccttt cggtttttct
    12121 ttttattata acaacatttt tccgttcccg caacagaaaa aaagcttccc ccgcagggga
    12181 aggcttttct cacttgttct tacttgtccg ccgggcggct ttgccggacg ttttcttctc
    12241 cgtctgggtg cgggtcgtct tgggggcggc tttcctggtg ccgggcttcg gggccggtgg
    12301 ttccttgtcc agcaggcgcg cgggcacggg atccagcttc cacagctggt tctcaccgta
    12361 aacgtcctgc cagatctggg cctgggtgcc gttttccacg cgcatgccca ccaggtcgat
    12421 gaccttatcg gccagcacat tgcgcagttt taccttgccg ccgccggcat ccacgatttg
    12481 ccagcgctgg cctgcgccgg atgtggccga ccactggtgt acccaggtgc cgttctcgac
    12541 gccggaaagc gccaggtcca tgaccttgcc ggtaaagcgg ttcttgatgc gatattcatt
    12601 ttccccggcc tctacaaaaa accattgctg ccagggctgg ccctcgtaac tccacaggcg
    12661 caccgaagca ccgttctcgg tgttgaaatc agccacctcc agcacgcggc cgttggcagc
    12721 agcgatggta tagcaggcat ccgggcggat gacagtttgg gcggatttct tcatggggat
    12781 tcctcctttg caagctgcgg gtgcagcttc tttttctcat tatagcatgg ttacgccgcg
    12841 cggtttagct aaacttt
//
LOCUS       DBMDSQ010000032        12062 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_1609_length_12062_cov_1.818243, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000032 DBMDSQ010000000
VERSION     DBMDSQ010000032.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 12062)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 12062)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12062
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1609_length_12062_cov_1.818243"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(272..1636)
                     /locus_tag="ACZPAX_02240"
     CDS             complement(272..1636)
                     /locus_tag="ACZPAX_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HIR1112750.1"
                     /translation="MEWILYVLLALAVAGDILLAVILAKVLQKNNETQQKIDTADDFV
                     RAMTPVLQSETDLLAEQLRAMQGESARTTSATLRDFSAVLAENQRQSAATNTARLEAI
                     DRAGAARQKAANDALLAQLTMMETRLKNLEDSNAVRMDSVRGAVVQGLNTIRADNNKK
                     LDEIRGTVEEKLQDTLQQRINDSFKTVSAQLEQVYKGLGEMQNLAADVGSLKQVLSGV
                     KTRGILGEVQLGAILEQILAPGQYACNVATVPGSANRVEYAVKLPGQSGTVWLPIDAK
                     FPGDTYAHLQAAQERGDAAAVAAMRKALNNVLRAEAKDIHDKYIEVPYTTSFGILFLP
                     FEGLYAEVVSSGVTELLQREYQITVAGPSTMAALLNALQMGFRTLAIQKRSGEVWTIL
                     GAVKTEFEKFGTGLQQMQRHLNQTGSDLEELIGPRSRAITRKLESVQQLDPDTATALL
                     GIEE"
     gene            complement(1646..2032)
                     /locus_tag="ACZPAX_02245"
     CDS             complement(1646..2032)
                     /locus_tag="ACZPAX_02245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HIR1112751.1"
                     /translation="MPTTLLIIYRILGVALFALAAAAIVEKYRTVRGAALLPARVLEC
                     RKAASTSPNRSAGGYRYLVELHANGQRLELETNDSFWFKHDKTVGKAIQVWYNPNARC
                     VERKSWETEIFSAVLAVIGAALLLIR"
     gene            2237..2539
                     /locus_tag="ACZPAX_02250"
     CDS             2237..2539
                     /locus_tag="ACZPAX_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007488616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YerC/YecD family TrpR-related protein"
                     /protein_id="HIR1112752.1"
                     /translation="MQQNERNTALYEAILRLNTLDECIRFFDDLCAVTELRTMEQRYA
                     VAGCLLQGKVYSEIRRETGASSATVSRVNRMLNYGTGSVAESVREDLAAQGIATEK"
     gene            2552..3601
                     /locus_tag="ACZPAX_02255"
     CDS             2552..3601
                     /locus_tag="ACZPAX_02255"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF008277.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HIR1112753.1"
                     /translation="MSVSIRDFGKSTTGETVKLAVLKNDFIEVQLLSYAAIIHRIIVP
                     NRKGNPVDVVLGYGTAADYELNTDSMGAAVGRFANRIAGAQFPLYEEIVHVTANQHGN
                     CLHSGLHGFSHSMFDVELTPGETDSVTMTAVSPAGTDGFPGNMTLTIQYSLAKSGLVI
                     RYGATTDAPTVCNMTNHSYFNLNGHASGSALGHRLTIDADYYLEADPYSIPTGRKLPV
                     GGTPMDFTEEKTLGLDIGADFAPLKECSGYDQCYIIRDSGLRHAAWAVGPETGIRMEV
                     LTTLPGMHLYSANFLAPVTDGKDGAHYAARDAVCFETEDFPDAPNHPNFPDTTLLPDQ
                     PYSSTTIYRFDLAEM"
     gene            complement(3857..4663)
                     /locus_tag="ACZPAX_02260"
     CDS             complement(3857..4663)
                     /locus_tag="ACZPAX_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007045864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1112754.1"
                     /translation="MLELKNIGKTFNSGTVNEKIALEGVNLHLDDGDFATIVGSNGAG
                     KSTLFNAIAGEFIADTGTITLAGKDITFLPDYKRSKAIGRMFQDPLRGTAPHMTIEEN
                     LALAFLRASGHTSPFSRITKKDRNLFAEKLSELGLGLEDRMKQPVGLLSGGQRQALTL
                     LMATLVTPKLLLLDEHTAALDPATADKVLKLTKKIVAENHLTCLMITHNMHDALTLGN
                     RTLMMDHGHIVLDIGGEERAHTTVDGLLDRFAENVGHALDNDRILLSKEA"
     gene            complement(4656..5573)
                     /locus_tag="ACZPAX_02265"
     CDS             complement(4656..5573)
                     /locus_tag="ACZPAX_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007045863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1112755.1"
                     /translation="MFTLATVLSALELGFIYALVALALFLSFRVLNIADMTTDGAFTL
                     GCAVSATAAVAGHPFLGLPLALLAGAAAGFVTAFLQTRLAVPSILAGIITNTGLYTVN
                     LAVMGFSSNVPMLKTTTIFTVAQSILGENAPYKLLVAALVTVVVCVLLILFLGTRMGL
                     SIRATGDNPDMVRASSINTAFTITVGLCISNAMTALSGAVLAQYQRSADINLGTGMVV
                     IGLASLIIGETLFGRSGMWVKAVAAVIGSVIYRFIIALALRANVPSECLKLISAVIVA
                     LAIAAPAIRSKAAFRRRRANAEKEGARHA"
     gene            complement(5720..6760)
                     /locus_tag="ACZPAX_02270"
     CDS             complement(5720..6760)
                     /locus_tag="ACZPAX_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007489481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR1112756.1"
                     /translation="MKKFVAVLSAAAMMTSLAACGSTADTTSTAASSSAAVSTESTAA
                     EATTDAKSYKIAVVQQLDHASLDEIRTAVEAELDAKAAEKGITIEYKDFNGQNDATTL
                     NQIGTQVVSDGYDAIIPIATLAAQCMATAAESTKTPVIYAAISDPAAADLTDIDYVTG
                     TSDALNTQSIMDMMFAVQPDIQTVGLLYSNSEANSTTPIAEAKAYLDAKGIAYVEKTG
                     NTNDEIMTAASSMVGQVDAVFTPTDNVVMAAAAAVSETLTKGGIPFYTGADSFVTAGA
                     FATCGVNYTELGTYTADMALDILETGTVPEYHVMDGGIITVNTETADALGIDYSAFND
                     LAGQVVEVTTAE"
     gene            complement(7271..9586)
                     /locus_tag="ACZPAX_02275"
     CDS             complement(7271..9586)
                     /locus_tag="ACZPAX_02275"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HIR1112757.1"
                     /translation="MNEVEIKKEEQTTEQSKPNFWRRLPRAGRIAMIAAAVVAVLAAV
                     AGLYINGKLDLLRYNDGSVEEMGDIDAGEDQDLDGTGLAHTEKEMTMPEGSPFSDDNV
                     LNILLIGTDERTEAVNDADAFTHLNQLDGTEDTTEFSDDARADSMILVSLDIKDHIIR
                     LVSIERGTGVPILLDGYEDQYDWITHTFRYGGPKLTMKTVEDCFNVQVDHYVRVNFNS
                     FVQIVNAVGGVDIDISELEAKALNWEVPSNSMLIVNHVDPGLNHFDGYTALQYARLRK
                     IDNDWKRVERQRTVIQAVLDQVKNASVVELDSLLNTVLPLIQTNFTKSEIAALLVQLP
                     GFLGCDVQQMSMPLQGTYGIRTGMDNRLMYDPDWVVNIKALQDFLYNDKTAEEVAAAT
                     PETAAAEAAGELVIATRETADVTDPVEEYNAKNLHRFDMTYPLDASDFGKDDYRVYVA
                     DTDNLRGSELRGALIERLYTSGVRVVAVPDGAAAGVLLDNYLQTGNTESLDGYLAVLP
                     AADRDSARTLWEGIYRSYPGVFHAAGLGADARSATVGKALSVLANASYNYPETEIAEA
                     VKALRSGTTADAVYWFKSAMASYPRQMERFFGDRYAAAARLYYAVQGTLNVADDSELP
                     LYDAKQLLKTYKNDAILIFTDEASAVQTEGGLAAELQAQLDKQKYDEGEDHDMVCAIA
                     TVYGTWSNAGNFTPDDAETAWDADSLTEYLGSDALRGKDMVLALDGEDSPYLNRQCLL
                     KDAGAPVGELVQKLFVLDKNNMASPETAQAE"
     gene            complement(9593..10915)
                     /locus_tag="ACZPAX_02280"
     CDS             complement(9593..10915)
                     /locus_tag="ACZPAX_02280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1112758.1"
                     /translation="MADTKQQKPPFPLRALAAFAVTLAALFVLLLAAYALPGDRVRDH
                     VYESAQTIAGEGLYPEYFGFKLFQMDNYTDTIMLTEAAAADEAPPLRAMMTNTAYNVD
                     NFETLADDLQWYIEKDWAAGAQRTDAPELEPFSYARYWHGYLIWLRPLLLVTTITGVR
                     VVQYVVLAALFAAVVWLLRRRCGLRAAFWFVVSQLLVTVFWVPHQVQYFTCFAAAYAG
                     CVWVLAKPRRSDSVCLALLVLGVVTAFCDLLVTPILTLGLPLVCWLLQPQQRLRAGVP
                     QCGIVVGGSLSWGVGYLLCWASKWVLAGLVTGQNVIADALHQVGVRTAADSWHGMELT
                     WGNIIRFVHETLAEKHLFWPLVLVAVLLAALFVASIRDKQQLVRALPLLLCTLMTPAW
                     FIVLRTHSIQHGWFTWRALGLTIFAGAAFLYYCCDIRAAGRRILHRGK"
     gene            complement(10996..12060)
                     /locus_tag="ACZPAX_02285"
     CDS             complement(10996..12060)
                     /locus_tag="ACZPAX_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007045857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0015648 - lipid-linked peptidoglycan
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HIR1112759.1"
                     /translation="MALLSYGLIMLFSAGYAVALYRRGDAYTYIRPQLLFAALGVAAM
                     YAASLVDYHIWHKLAWPMLGLSLVLLTVVLFMPEYNGCKRWIVLPGLGTLQPSEIAKF
                     SVVLVFSHIISLNHDRMKTFSTGVLPFALILGTVTVLMLLEPHLSGTLLILSIGAVLM
                     FVGGTGLQWFGLAGGLGVAAIAAAVAALPELVPYATDRLASWRDPFADPLGEGHQTIQ
                     SLYAIASGGLAGLGLGNSRQKYLYVPEPQNDFIFSILCEELGFFGAALAVALFLLLLL
                     RGMFIAVRARDKFGALLTVGFVVQVILQAILNIAVVTNTIPNTGISLPFFSSGGTSLL
                     MLLGEMGIVLSVSRQTDMVS"
BASE COUNT         2354 a         3675 c         3667 g         2366 t
ORIGIN      
        1 tcaataactc attcagtata ccacagcgca cccgccgtgt aaagtgggca gcggcataga
       61 aaaagccccc ttgcgggggc ggattctggc aaaaatgaca gcgtagaaag caaacagcca
      121 cactgtaggg gccgggcatg cccggccctc atcttgaagg cagacggtcg tttgcgggaa
      181 agttgcgggc cgcatatatg cggcccctac cgggcggcgt ttgtttgagc cgagcaataa
      241 cggcacacag cccgacaggg atactcctcc cctactcctc gatgcccaat agcgctgtgg
      301 ccgtatccgg gtcaagctgc tgcaccgact ccagcttgcg ggtgatggcg cggctgcggg
      361 ggccgatgag ttcttccagg tcagagccag tctggttcag gtggcgctgc atctgctgca
      421 agccggtgcc aaacttttca aactcggtct tgaccgcacc caagatcgtc cagacctcgc
      481 cggagcgctt ctggatcgcc agcgtgcgga agcccatctg caaggcgttg agcagcgccg
      541 ccatggtgga ggggcctgca accgtgatct gatactcgcg ctgcagcagt tcggtcacac
      601 cgctggagac gacctcggca tacagcccct caaacggcag gaacaggatg ccgaagctgg
      661 tggtgtaggg tacctcgata tacttgtcat ggatatcctt tgcctcggcg cgcaggacgt
      721 tattcagtgc cttgcgcata gcggccacgg cggccgcatc gccccgctcc tgcgctgcct
      781 gcaaatgggc gtaggtatcg ccggggaatt tcgcgtcgat gggcagccag accgtgccgc
      841 tctggcccgg cagcttgacg gcatactcca cgcggttggc actgcccggc acggtcgcca
      901 cattgcaggc atactggccg ggggccagaa tctgctccaa aatcgcgccg agctgcactt
      961 cgcccaggat gccgcgggtc ttgacgccgg agagcacctg cttcaggctg cccacatcgg
     1021 cggccaaatt ctgcatctcg cccaggccct tgtagacctg ttccagctga gcgctgaccg
     1081 tcttgaacga atcgttgatg cgctgctgca aggtgtcctg cagcttttct tcgacggtac
     1141 cgcgaatttc gtccagcttt ttgttgttgt cggcgcggat ggtgttcaag ccctgcacca
     1201 ccgcgccgcg cacgctgtcc atccgcacgg cgttggaatc ttccagattt ttcaaccgcg
     1261 tttccatcat tgtcagctga gccagcaggg cgtcgttggc agccttctgg cgggcagccc
     1321 cggcgcggtc gatagcctcc agccgtgcgg tattggtggc cgcactctgg cgctggttct
     1381 cggccagcac ggcggagaaa tcccgcagtg tggcgctggt ggtgcgggcg ctctccccct
     1441 gcatggcgcg cagctgctcg gccagtaggt ccgtctcgct ttgaagcacc ggggtcatgg
     1501 cgcggacaaa gtcgtccgcc gtgtcaattt tctgctgtgt ttcgttgttt ttttgcagta
     1561 cctttgccag aatgaccgcc aacaggatat cccccgccac ggccagcgcc aacagaacat
     1621 ataatatcca ttccatgctc cgccctcacc gtatcagcag cagcgccgca ccgatgacgg
     1681 ccagcactgc cgagaagatc tccgtctccc agcttttgcg ctcgacacag cgggcgttgg
     1741 ggttgtacca gacctggatt gccttgccca cggtcttgtc atgcttgaac cagaaggagt
     1801 cgttcgtttc cagctccaac cgctggccgt tggcgtgcag ttccactaag tagcggtagc
     1861 cgcccgcgct gcggttgggg ctggtggagg ctgccttgcg gcattccagc acgcgggcag
     1921 gcagcagcgc cgccccgcgc acggtgcggt atttttccac aatcgcggcg gccgccagtg
     1981 caaaaagcgc aacgccaagg atgcggtaga tgataagcaa tgttgttggc atagttttct
     2041 ccctctgttt tggtatttct atgatacaac attttggtgt taaatgcaag ctattctttt
     2101 tatattttat gatgctttca cgctttagtg tattgacttg attggtcaaa tttgctagaa
     2161 tgtagagaga tagaccccga aacgaatgca aattttacaa agaaagaggt cggcgcatcc
     2221 cctgcgccac tacgctatgc aacaaaatga acgtaatacc gccctgtatg aggcgatcct
     2281 gcggctgaac acgctggatg agtgcatccg cttttttgac gacctgtgcg ccgtgaccga
     2341 gctgcgcacg atggagcagc gctacgctgt ggccggctgc ctgttgcagg gcaaggtgta
     2401 cagcgaaatc cgccgcgaga ccggcgcatc cagcgccacc gtcagccgcg tgaaccgcat
     2461 gctgaactac ggcaccggca gcgtggccga gagcgtgcgc gaggacctgg ccgcccaggg
     2521 tattgccacc gaaaagtaag gaggttcccg tatgtctgtt tcgatccgtg atttcggcaa
     2581 gtccaccacc ggcgagaccg tcaagctggc cgtgctgaaa aatgacttta tcgaggtgca
     2641 gctgctgagt tatgccgcca ttattcatcg tatcatcgtg ccgaaccgca agggcaatcc
     2701 ggtggatgtg gtgctgggct acggcactgc cgccgactac gagctgaata ccgacagcat
     2761 gggggccgcc gtgggccgct ttgccaaccg cattgcgggg gcgcagttcc ccctgtatga
     2821 ggagatcgtc catgtgacgg caaatcagca cggaaactgc ctgcacagcg gcctgcacgg
     2881 gttcagccac tcgatgtttg atgtggagct gacccctggc gagaccgaca gcgtgacgat
     2941 gaccgccgtc agccccgcgg gcaccgacgg ctttcccggc aacatgacgt tgaccatcca
     3001 gtacagcctg gcaaagagcg ggctggtcat ccgctatggg gccaccactg atgcgcccac
     3061 cgtctgcaat atgacgaatc acagttattt taacctgaac ggccacgcca gcggcagcgc
     3121 cctgggccac cgcctgacca ttgacgccga ctattatctt gaggccgacc cgtacagcat
     3181 ccccaccggg cgcaagctgc cggtgggggg cacgccgatg gattttaccg aggaaaagac
     3241 cctgggtctg gacattggcg ctgactttgc cccgctgaag gaatgcagcg gctacgacca
     3301 gtgctacatc atccgggaca gcggcctgcg ccacgcggcc tgggccgtcg gccccgagac
     3361 cggcatccgc atggaggtat tgaccaccct gcccggtatg cacttgtatt cggccaactt
     3421 cctggcaccg gtcacggatg gcaaggacgg cgcacactac gccgcccggg acgccgtctg
     3481 ctttgagacg gaggactttc ccgacgcacc gaaccatccg aacttcccgg acacgacgct
     3541 gctgccggac cagccgtaca gctccacgac gatttatcgg tttgatttgg cggagatgta
     3601 atcttcctcg ccttcccctt ggggctgcgc ccgcaggcgc gtgtcgaagc gcaaccgccg
     3661 caggcggctc ttagcgcgaa gactgaaggt ggccccgcgg ggccggatga ggggcaagtt
     3721 tttggatatt gcccgtaagc gggaagcacc atagactttg ccctcatcag tccgctatgc
     3781 ggacagcttc ccccgagggg gaagccttta cctacaaaaa ccgccctgcg cgaaaatttc
     3841 gtgcagggcg gtttttttat gcctctttac tcaacaaaat tctatcatta tccagcgcgt
     3901 ggccgacgtt ctcggcaaag cggtccagca gaccgtccac cgtggtgtgg gcgcgctcct
     3961 cgccgccgat gtccagcacg atgtggccat ggtccatcat caatgtacgg ttgcccaggg
     4021 tcagcgcgtc gtgcatattg tgggtgatca tcaggcaggt caggtggttt tcggccacga
     4081 tctttttcgt cagtttcagc accttgtccg ccgtggcggg atccagggcg gcggtgtgct
     4141 cgtccagcag cagcagtttc ggcgtgacca gcgtggccat cagcagggtc aatgcctggc
     4201 gctggccgcc ggacagcagg cccaccggct gcttcatccg gtcctccagg ccaaggccca
     4261 gctcggagag tttttccgca aagaggttgc ggtccttctt cgtgatgcgg gagaagggcg
     4321 acgtgtgccc cgatgcccgc aggaaggcca gcgcaagatt ttcctcaatg gtcatatgcg
     4381 gggccgtgcc gcgcagcggg tcctggaaca tccgaccgat ggccttggag cgcttgtagt
     4441 ctggcaaaaa ggtgatatct ttgcccgcca gcgtgatggt gccggtgtcg gcgatgaact
     4501 cgcccgcaat ggcgttgaac agcgtggatt tgcccgcgcc gttggagccg acgatggtgg
     4561 caaagtcacc gtcgtcgagg tgaaggttca cgccttccag cgcgatcttc tcattgacgg
     4621 tgccggagtt gaacgttttg ccgatgtttt tcagttcaag catggcgggc accttctttc
     4681 tcagcgttgg cacggcggcg gcggaaagcc gccttggacc ggatggcggg cgcagcgatg
     4741 gccagcgcca cgatgacggc ggaaatcagt ttcagacact ctgacgggac gttggcacgc
     4801 agggccagcg caatgatgaa acggtagata acgctgccga taacggcggc cacagccttg
     4861 acccacatac cgctgcggcc aaacagcgtc tcgccgatga tgagggacgc caggccgatg
     4921 acgaccatgc cggtaccgag gttgatatcc gccgagcgct ggtactgggc cagcaccgcg
     4981 ccggacaggg cggtcatggc gttggagatg cagaggccta ccgtgatggt gaacgccgtg
     5041 ttgatggagg acgcgcggac catgtccggg ttgtcgccgg tggcccggat ggacaggccc
     5101 atccgagtgc cgaggaacag gatgagcagc acgcagacca caactgtgac aagggccgcc
     5161 accagcagtt tatagggtgc attctcgccg aggatgctct gcgccacggt gaagatcgtg
     5221 gtcgttttca acataggtac gttggaagaa aaccccatga ccgccaggtt gacggtgtac
     5281 agccctgtgt tggtgatgat gcccgccaga atggacggca ccgccagccg cgtttgcaga
     5341 aacgccgtga cgaacccggc cgccgcaccg gccagtaagg ccagcggcag acccaggaac
     5401 ggatggcccg ccaccgcggc ggtagccgaa acagcgcagc ccagggtgaa tgcgccgtcg
     5461 gtggtcatat cggcgatgtt cagcacgcgg aagctgagga acagcgccag ggcaaccagc
     5521 gcgtagataa agcccagctc caacgcgcta agcacagtgg caagggtgaa catggggtaa
     5581 cttcctctct aaaagtaatg gtacagtcta ttatacaaca atgtaggggc ggattccata
     5641 tccgcccgcg ggacgctgcg gctgcgcagg catcccgggc gcatatagaa tgcgccccta
     5701 cggtctcttc ccggagggtt tattccgccg tggtgacctc aacgacctga ccggccagat
     5761 cgttgaaggc gctgtaatcg atgcccaggg cgtcggcggt ctcggtgttg acggtgatga
     5821 tgccgccgtc catgacgtgg tactcaggca cggtgccggt ctcgaggata tcgagggcca
     5881 tgtcggcggt gtaggtgcca agctcggtgt agttgacgcc gcaggtggcg aacgcgccgg
     5941 ccgtgacgaa gctgtccgca ccggtataga atgggatgcc gcccttggtc agtgtctcgg
     6001 agacagcggc ggcagcggcc atgacgacgt tatcggtcgg ggtaaagacg gcgtcgacct
     6061 ggcccaccat gctggacgcg gcggtcatga tctcatcgtt ggtgttgcct gtcttttcga
     6121 cgtaggcaat gcccttggca tcgaggtaag ccttggcttc ggcaatgggg gtcgtggagt
     6181 tggcttcaga gttggagtag agcaggccga cggtctggat gtcaggctgg acggcaaaca
     6241 tcatatccat gatggactgg gtgttcagcg cgtcggaagt gccggtgacg tagtcgatat
     6301 cggtcagatc cgcggcggcg gggtcagaga tggcggcgta gatgaccggg gtcttggtgc
     6361 tctcagcagc ggtggccatg cactgggcag ccagcgttgc aatggggatg atggcgtcgt
     6421 agccgtccga gacgacctgg gtgccgatct gattcagcgt cgtggcgtcg ttctggccgt
     6481 tgaagtcctt gtactcaatg gtgatgccct tctcggcggc cttggcgtcc agctcggcct
     6541 cgacggcggt gcggatctcg tccaggctgg cgtggtcgag ctgctgcacc acggcgatct
     6601 tgtagctctt ggcgtcggta gtggcttcag cggcggtgct ctcggtggag acggcggcgg
     6661 aggaggaagc ggcggtggag gtggtatcag cggtggagcc acaggcggcc aggctggtca
     6721 tcatagcggc agcggacaga acagcaacga actttttcat ggtaaactct cctttatttg
     6781 taaaacaggt aatgttgtag ttgcaggggc ggtcatggac cacctgcggg cgaataggtc
     6841 tgcggactta ttttacaaat tcaggcttgc catcgctctt tcatcaggtc tatcttcatg
     6901 tttccctctc ccctcaaagt tgaaaaacaa aactgccctt gcacagaatt tctcctgtgc
     6961 aagggcagat aaaacaaagc tgtggtgcca ccttgcttgc cgtgcatagt ataaaaacac
     7021 ggccactccg tagggaacca acattcccct gctctataac gggagcaccc ggcgctggat
     7081 actgggagcg aggccccttt ccccatgccc tccgcgaacc attatgctta cccgctctgt
     7141 atccccttcc cagcgtcggg gactctctgt gactgcgctg tgagctttac tttcgcttca
     7201 ttggtttaaa gcatttatac cacaagccgg tgcgtttgtc aacacccttt tgcaaatttt
     7261 cttgcggtgt ttattctgcc tgcgctgttt ccggcgaagc catgttgttt ttatcgagta
     7321 caaacagttt ttgcaccaac tcccccaccg gggcaccggc gtcctttaac aggcattggc
     7381 ggttcaggta ggggctgtcc tcgccgtcca gcgccagcac catatccttg ccgcgcagcg
     7441 catcgctgcc cagatattcg gtcaggctgt cggcgtccca ggcagtctcg gcgtcgtcgg
     7501 gggtaaagtt cccagcgttg ctccacgtgc cgtagaccgt ggcgatggcg cagaccatgt
     7561 cgtgatcctc gccctcgtca tacttctgct tgtccagctg ggcctgcagt tcagcggcca
     7621 ggccgccctc ggtctgcaca gcgctggctt cgtcagtgaa gattaaaatc gcatcattct
     7681 tataggtttt cagcagctgt ttggcgtcgt agagcggcag ttcgctgtcg tcggccacgt
     7741 tcagcgtgcc ctgcacggcg tagtacaggc gggccgcggc ggcgtaacgg tcgccgaaga
     7801 accgctccat ctggcgcggg tagctggcca tggcggactt gaaccaatac acggcgtcgg
     7861 cggtcgtgcc gctgcgcaga gcttttaccg cctcggcgat ctcggtttcg gggtagttgt
     7921 agcttgcgtt cgccagcaca gacagcgctt tgcccacggt ggcgctgcgg gcgtcagccc
     7981 ccaggcccgc cgcgtggaac acgccggggt agctgcggta gatgccctcc cacagggtgc
     8041 gggcactgtc acggtcggcg gcgggcagca cagccaggta gccgtccagg ctctctgtat
     8101 tgccggtctg caggtagttg tccaacaaca cgccggcagc cgcgccatcg ggcacggcca
     8161 cgacccgcac accggaagtg tacagccgct cgatcagtgc gccgcgcagt tcgctcccgc
     8221 gcaggttgtc ggtgtcggcc acgtagacgc ggtagtcgtc cttcccgaaa tccgacgcat
     8281 ccaacgggta ggtcatgtcg aagcgatgca agtttttggc attgtactcc tctaccgggt
     8341 cggtcacgtc ggcagtctcc cgggtggcaa tgaccagctc gcccgcagcc tcggcggcgg
     8401 cggtctccgg cgtggcggca gcgacttcct cggcggtctt atcgttgtac aaaaagtcct
     8461 gcaaggcctt gatgttcacg acccagtcag ggtcgtacat caggcggttg tccatgccgg
     8521 tgcggatgcc gtaggtgcct tgcaaaggca tcgacatctg ctgcacatcg cagcccaaaa
     8581 agcccggcag ctgcacaagc agggcggcga tctcggactt ggtaaagttt gtctggatca
     8641 gcggcagcac ggtgttcagc aggctgtcca gctccacgac actggcgttt ttcacctggt
     8701 ccagcacggc ctggatgacg gtgcgctgac gctcgacgcg cttccagtcg ttgtcgatct
     8761 tgcgcagacg tgcgtattgc agcgccgtgt agccgtcaaa gtggttgagg cccgggtcaa
     8821 cgtggttgac aataagcatc gagttggagg ggacctccca attcagcgcc ttggcctcca
     8881 gttcgctgat gtcgatatcg acgccgccga cggcattcac gatctggaca aaggagttga
     8941 aattgacgcg aacatagtgg tcgacctgga cgttgaagca gtcctcgacg gttttcatcg
     9001 tcagcttggg gccaccgtag cggaaggtgt gggtgatcca gtcgtattgg tcctcgtagc
     9061 cgtccagcaa aatcggtacg ccggtgccgc gttcgatgga gacaaggcgg atgatatgat
     9121 ccttgatgtc cagcgaaacc aaaatcatgc tgtcggcacg ggcatcatcg ctgaactcgg
     9181 tcgtatcctc ggtgccgtcc agctggttca ggtgggtgaa ggcatcggca tcgttgacgg
     9241 cctcggtgcg ttcgtcggta ccaatgagca ggatgttcag cacgttgtcg tcagagaaag
     9301 ggctgccctc gggcatcgtc atctcttttt cggtgtgggc caggccggtg ccgtccaggt
     9361 cctggtcctc gcctgcgtca atatcgccca tttcctccac gctgccgtcg ttgtagcgca
     9421 gcaggtcgag cttgccgttg atgtacagcc cggccacggc cgccagcacc gccacgaccg
     9481 ctgccgcaat catggcgata cgcccggccc tcggcaagcg gcgccaaaag ttgggctttg
     9541 actgttcggt ggtttgttct tcttttttga tttctacttc gttcatggtt gtttactttc
     9601 ccctatgcaa aatacgccgc cctgcggcac gaatatcaca acaataatac aaaaatgccg
     9661 ccccggcaaa tatggtcagg cccagcgcac gccaggtgaa ccaaccgtgc tggatgctgt
     9721 gggtgcgcag cacgatgaac cacgccgggg tcatcagtgt gcagagcagc agcggcagcg
     9781 cccggacaag ctgttgctta tcccggatgc ttgccacaaa cagcgccgcc agcagcaccg
     9841 ccacaaggac cagcggccag aacaagtgct tttccgccaa tgtttcgtgt acaaaacgta
     9901 ttatattacc ccatgtcaat tccataccgt gccagctgtc ggcggcggtg cgcacgccca
     9961 cctggtgcag cgcgtcggca atgacgttct gcccggtcac aagcccggcc aacacccatt
    10021 tggacgccca gcacagcaga tacccgacgc cccatgaaag gcttccgccc accacaatgc
    10081 cgcactgggg cacacctgcc cgcagccgct gctggggctg cagcagccag cagaccagcg
    10141 gcaggccgag cgtcaggatc ggcgtgacga gcaggtcgca gaaggccgtc acaacgccca
    10201 aaaccagcaa ggccaggcag acgctgtcac tgcggcgggg cttggccagc acccagacgc
    10261 agccagcata ggcggcggca aagcaggtga aatactgcac ctgatgcggc acccaaaaca
    10321 ccgtgaccag cagctggctg acgacgaacc agaaggccgc ccgcagcccg cagcggcggc
    10381 gcagcagcca caccaccgcc gcaaacagcg ccgccagcac cacatactgc acgacgcgca
    10441 cgccggtaat ggtcgtgacc agcagcagcg gtcgcagcca aatgagatac ccgtgccagt
    10501 accgcgcgta ggagaagggt tccaactccg gtgcgtcagt gcgctgcgcc ccggcggccc
    10561 aatccttttc tatgtaccat tgcaggtcgt cggccagcgt ctcaaagtta tcgacgttgt
    10621 aggccgtgtt cgtcatcatg gctctcagcg gcggggcctc gtcggcagca gcggcctcgg
    10681 tcagcatgat ggtgtcggta taattgtcca tctggaacag cttgaagccg aaatattcag
    10741 ggtacagccc ctcacctgcg atggtctgcg cggattcgta gacgtggtcc cgcacccgat
    10801 ccccaggcag ggcgtaggcc gccagcaaca gcacaaacag cgccgccagc gtaacggcaa
    10861 acgccgccag tgcccgcagg ggaaaaggtg gtttttgttg tttcgtgtcc gccatggggc
    10921 acctctttcc gataaaaaat ctattctatt ataaaacaaa agtcccccgg tttcaaccgt
    10981 gggactgtga aaaaactatg acaccatatc cgtctgccgg gatacggaca gcacaatgcc
    11041 catttctccc agcagcatca gcaagctggt accgccggag gaaaaaaacg gcaggctgat
    11101 acccgtattc ggtatcgtgt tcgttacgac cgcaatgttc aaaatggcct gcaaaatgac
    11161 ctgcaccaca aagcccaccg tcagcagcgc accaaactta tcccgcgccc gcaccgcaat
    11221 gaacataccg cgcagcagca gcagcaaaaa cagcgctacc gccagtgccg cgccaaaaaa
    11281 gcccagctcc tcgcacagga tcgagaagat gaaatcgttc tgcggctcgg gtacatacaa
    11341 atatttctgg cggctgttgc ccagccccag cccggccagc ccgccggacg cgatggcgta
    11401 caggctctgg atcgtctggt gtccctcccc cagcgggtcg gcgaagggat cccgccagct
    11461 ggccaggcgg tcggtggcgt aggggaccag ctccggcagg gccgccaccg ccgccgcgat
    11521 ggccgccacg cccaggcccc ctgccaggcc gaaccactgc agcccggtcc cacccacaaa
    11581 catgagcacc gcgccgatgg acaaaatcag cagcgtgccg gacaggtgcg gctccaacag
    11641 catcagcacc gtgaccgtgc ccagaatcag cgcgaagggc agcacgccgg tggagaaggt
    11701 tttcatcctg tcatgattca gcgatatgat gtgggaaaac accagcacca ccgagaattt
    11761 tgcgatctcg ctgggctgca aggtacccag ccccggcagc acgatccacc gcttgcagcc
    11821 gttgtactcc ggcataaaca gcactaccgt gagcaaaacc agcgacagac ccagcatcgg
    11881 ccaggccagc ttgtgccaaa tgtggtagtc caccaggctg gccgcgtaca tggccgccac
    11941 gcccagcgcc gcaaacaaca gctgcggccg gatgtaggtg taggcgtcgc cgcggcggta
    12001 cagcgccacc gcatacccgg ccgaaaacag catgatcagc ccataactga gcagtgccag
    12061 ca
//
LOCUS       DBMDSQ010000033        11862 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_1630_length_11862_cov_2.028059, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000033 DBMDSQ010000000
VERSION     DBMDSQ010000033.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 11862)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 11862)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11862
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1630_length_11862_cov_2.028059"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1853
                     /locus_tag="ACZPAX_02290"
     CDS             <1..1853
                     /locus_tag="ACZPAX_02290"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005923613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 2 protein"
                     /protein_id="HIR1112760.1"
                     /translation="APEKAAVLRVSLCAPDGAALVGQQLPAAAKAECTLTLPDAQPWD
                     TDHPNLYTCRAELLDAAGQVLDCRETTFGFRMAEFKADGFYLNGKKTFLRGLNRHQSY
                     PYIGYAAPESLQRNDARILKNELHCNAVRTSHYPQSQHFLDECDKLGLLVFTELPGWQ
                     HIGDAAWKDRACEMLREMILQNRSHPSIILWGVRINESVDDDEFYKRTNNIAHELDPS
                     RATSGVRYLEKSHLLEDVYAYNDFSHNGMNAGAKRKKDVTPDMGKALLISECNGHMYP
                     TKPFDDAPHRQEHALRHARVLNAAYADGEHAGCFGWCMFDYATHKDFGSGDRICYHGV
                     LDSFRNPKLAAAVYASQGGGKPILTVSSTMDIGDSPAGQLGTVYVFTNAERVDLYKND
                     VFVTTLHKSGWTALPHPPLAVDDTIGELLETQERFDKAKADTLRDCLLAAGKYGLAGL
                     PLRYKFRLAWCMVRYKMSFDDGVKLYGKYVGNWGGAATRWRFDAKNGDTVVKSVTLCP
                     SRKLHLEVTPSAHALREGDTYDMAAVRVRILDENGSPAAYAQLPLQFAASGAAALVGP
                     SIATAEGGMGGTYIRTIGQTGTASLTVAAPQCEPVTVTFSVTGKENTAWN"
     gene            1844..3226
                     /locus_tag="ACZPAX_02295"
     CDS             1844..3226
                     /locus_tag="ACZPAX_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005934070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015486 -
                     glycoside-pentoside-hexuronide:cation symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide (GPH):cation
                     symporter"
                     /protein_id="HIR1112761.1"
                     /translation="MELTTKQKAAFGIGAVGKDMVYALSASYVMYYYQDVLGLSATFV
                     GLILMIARVFDAFNDPFMGVLVAKTRTRWGRFRPWIFSGTVLNAAVLYALFAAPALAE
                     AGMMVYFSVIYILWGVTYTMMDIPYWSMIPAVTRTPADRENLSVVGRTCAGVGSALIA
                     MFTMLLVGALGGDSERAGFRWVALIVAVIFVVTEILCCASVKETSGGEMKTATVGEMF
                     KALFANDQALVVVGSIVLINSALYLTSNFIIYFFKYDFGGTGWKASYTLFSTIGGAAQ
                     ILGMMILYPLVRKKLSNTGVFTLSLALALGGYGVLLVLCLAGFSGNLVLLCVPGVVVF
                     ACNGMLSVLTTLFLSNSVDYGQLKTGRREESVIFSMQTFVVKAASGVAVFLTGIGLDL
                     IGLVGNTEETGPVAAQSASTLLGLRLMMTVLPMVVLVAALVLFRRKFTLSDDHAAEIS
                     AQLHGKKEGV"
     gene            3228..4829
                     /locus_tag="ACZPAX_02300"
     CDS             3228..4829
                     /locus_tag="ACZPAX_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005934073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0004557 - alpha-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 36 protein"
                     /protein_id="HIR1112762.1"
                     /translation="MTELHWRAATDAGSYDGTASISVGQGAVHLRTDLPAGTLENAVA
                     TLSWPMAENEKIFMNGYQTWTECPELPKWGRQRGTDHIPKFLLDKYAFDRYGDYHFVR
                     YSKRKGMSHGFSYCYFRSGEHYRLVASLDETPGYTIFYYDAKAAVLRIERDCAGVCHG
                     GSVFAAFDLFFTEGSEDEVFDAWFAALGCRPRTTKKLAGYSSWYNRYQNIDEASILED
                     LQGCRALFKPGDLFQIDDGWEAKVGDWLETDPAKFPHGLRPLADAAHDSGFLTGLWLA
                     PFVCEKESAVYRDHPDWLLQVNGQPWCCGSNWSSFYALDIDNPEVLDYLQTVFDRVLN
                     DWGFDLVKLDFLYGAATFGSASESRAARMQRAIALLRTWCGDKLILGCGVPVMPAFGV
                     VDYCRIGCDVSLDWDDVWYMRLFHRERVSTKQSIGNTVFRRQLNGRAYGSDPDVFFLR
                     EENCKLTPQQKDQLATVNALFSGILLTSDMPSRYTLEMREQYAALRDLSAHAENICVD
                     ADHDICIRYTLHGQEKTLVLEKKKL"
     gene            5183..5389
                     /locus_tag="ACZPAX_02305"
     CDS             5183..5389
                     /locus_tag="ACZPAX_02305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1112763.1"
                     /translation="MKFSPPVEEVKALTARGEYKVAPISCEILSDICTPIESMQILKN
                     VSTHCYLLESPGQRKVGPLHVFGL"
     gene            5412..6140
                     /locus_tag="ACZPAX_02310"
     CDS             5412..6140
                     /locus_tag="ACZPAX_02310"
                     /inference="COORDINATES: protein motif:HMM:NF016593.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1112764.1"
                     /translation="MNGTLRAGDVTLHTDDPSAVLRDILARYKSQRLPGLPTFTGGLV
                     GYFSYDYLGYREPKARTDAQDTENFKDVDLMLFDKVIAFDNLRQKIILIANMPLDAVQ
                     TNYNKATRELQQMADLLRHGTKKQEPPGKLLGEFTPLFDRAAYCAMVERARHHIYEGN
                     IFQIVLSDRLSAPFDLYQGDSKYRRGKLSAKNFGMEDNKKLYRCTLHFACDNRTPWQN
                     LYAGALAEWLPATFFLSFQKMVVK"
     gene            6239..7102
                     /locus_tag="ACZPAX_02315"
     CDS             6239..7102
                     /locus_tag="ACZPAX_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008119074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HIR1112765.1"
                     /translation="MEIGQKVKIISDSTCDLSQELTAKYDIDILPLHILLGENEFRDG
                     KDTTPDRIFAWADANKATPKTSAPSLEDAKVLLQPYVEAGREVICFSISGSMSTSGNV
                     MRLAAEDLDAAAQVTIIDSENLSTGIGLLVLEAAILAEQGHTAAEITAAVRKLIPKVR
                     ASFVVDTLVYLWRGGRCNAVAALAGSALRLHPKIVVENGAMRATKKYRGKIDNVIMAY
                     VKDMEADLKAARPERVFITHSGCSREVVGEVRSYLKELNVFCEILETRAGGVISSHCG
                     PGTLGVLFIAK"
     gene            7220..7468
                     /locus_tag="ACZPAX_02320"
     CDS             7220..7468
                     /locus_tag="ACZPAX_02320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1112766.1"
                     /translation="MADSLMTCTPYGINTHRLIVQARRVAYNGEDDAPAKTVQAIIHY
                     TVRSFKRTYTLYIGAAVILFLVVVLFRRGQRKRKTHKK"
     gene            complement(7794..7949)
                     /locus_tag="ACZPAX_02325"
     CDS             complement(7794..7949)
                     /locus_tag="ACZPAX_02325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1112767.1"
                     /translation="MAAAKKTRCDDFITALPDGYNTVIGEGGASLSGGEKQRISIAQR
                     AMDLLTD"
     gene            complement(8072..8419)
                     /locus_tag="ACZPAX_02330"
     CDS             complement(8072..8419)
                     /locus_tag="ACZPAX_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006574841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HIR1112768.1"
                     /translation="MKIAVTYADGQVYQHFGHAAQFKLYTVESGMILDTAVVDATASG
                     HSAMVSYLQRQGVNTVICGGIGGGAVSALAGAGIRLFAGTTGSADQAVLALLAGKLVH
                     NPEAAKGHCGGHH"
     gene            9095..9592
                     /locus_tag="ACZPAX_02335"
     CDS             9095..9592
                     /locus_tag="ACZPAX_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HIR1112769.1"
                     /translation="MLHIYYGDGKGKTTAAMGLALRALGHEKHVTILQFLKDGSSGEI
                     ALLRRCGADVHACPNAKFTWLMTADEKAEAKEHNNAALRTALAAPFDLLVLDEACAAC
                     RTGLVDESLLQQAAALAGQGREVVFTGRNPAAWMLEQADYATEMRAEKHPYSKGVAAR
                     EGVEF"
     gene            9781..>11862
                     /locus_tag="ACZPAX_02340"
     CDS             9781..>11862
                     /locus_tag="ACZPAX_02340"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004066990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 protein"
                     /protein_id="HIR1112770.1"
                     /translation="MDEIKAQKKALKKAYKKAKRKTVLGWKILTILCAILMVVFIPLC
                     TILNTFDNTVAAFVGGTFWKLENEDPNAQYFTTDFSSPEEMVDYGLTVAQQVEAEGAA
                     LLMNNGALPLAKDAKVSTFSSSSVNLVYGGTGSGNIDASTADTLRTALEKVGVTVNPT
                     LWDFYATGAGKDYTRANGGTVSTASATTAEVPWDVYTDDVKSSVAQYGDAAIVTLSRV
                     GGEGADLSYGEVNYLALDDNEKSMLENLASMKKNGEIKSIIVLINSANALQVDFLKNN
                     DYDVDAALWIGDVGISGINAVAQILVGDVNPSGSLVDTYCYDNYSAPAMWNFTPSVYD
                     GYVEGGDVPAKGSTYMIYQEGIYVGYKYYETRYEDYVMGAGNAGDYAYGDIVAYPFGY
                     GLSYTNFKFSDMTVNYNDALDVYTVAVKVTNTGAVAGKRTVQLYVQSPYTDYDKQNGV
                     EKAAVALVGFGKTGELAPGTSEIVTMNVNKRDIASYDTYGAGTYILDAGDYYFTAAND
                     AHEAVNNILAAKGYTVASTNGKMTADGDADLTYAWTEDKLDTTTYSVSENGTAITNQL
                     SCADPNLYDGEDNTVTWLSRSDWNGTLPTETVKLALTEQLKKDLQDIRYDPADYEAVN
                     LPAMGKNNGVTLYDMIGLDYDDPKWDDLLDNLTFDEMNTLIGDAFHWTMPVKSIEAPG
                     TRDENGPQGLTA"
BASE COUNT         2697 a         3400 c         3423 g         2342 t
ORIGIN      
        1 atgctccgga aaaggctgcc gtgctgcggg tgagcctctg cgccccggac ggcgcggcac
       61 ttgtgggcca acagctgccc gccgccgcaa aagcagaatg cacgctgacc ctgcccgacg
      121 cccagccctg ggataccgac cacccgaacc tgtacacctg ccgcgctgaa cttctggacg
      181 cggccgggca ggtactggac tgccgtgaga cgacattcgg cttccgcatg gcagaattta
      241 aggcggacgg attttattta aatgggaaaa agacgttcct gcgcggactc aaccgccacc
      301 agagctaccc ttacatcggc tacgccgcgc cggagagctt gcagcgcaac gatgcccgca
      361 ttttgaaaaa cgagctgcac tgcaacgccg tccgcaccag tcattacccg cagagccagc
      421 actttctgga cgaatgtgac aagctgggac tgcttgtctt taccgagctg cccggctggc
      481 agcacatcgg cgacgccgca tggaaggacc gcgcctgcga aatgctgcga gagatgatcc
      541 tgcaaaaccg cagccacccc agcattatct tgtggggcgt gcgcatcaat gagagtgtgg
      601 acgacgatga attttacaaa cgaacgaata acattgcgca tgagcttgac ccgagccgcg
      661 caacctccgg tgtgcggtac ctggaaaaaa gccatttgtt agaggacgtc tacgcctaca
      721 acgatttctc ccacaacggc atgaacgccg gggccaagcg caaaaaggac gtgacccccg
      781 atatgggcaa agcgctcctg atttcggagt gcaacggcca tatgtacccc acaaagcctt
      841 ttgacgatgc gccccaccgc caggaacacg cgctgcgcca tgcgcgggta ctgaacgccg
      901 cttacgccga cggcgaacat gcgggctgct tcggctggtg tatgttcgac tacgccaccc
      961 acaaggattt tggctccggt gaccgcatct gctaccacgg cgtgctggac agcttccgca
     1021 acccgaaact ggccgccgcc gtctatgcct cccaaggcgg gggcaaaccg attttgaccg
     1081 tcagctcaac gatggacatt ggcgattccc cggctgggca gttgggtacg gtgtatgtgt
     1141 tcacgaacgc cgagagggtg gacctctaca aaaatgacgt atttgtgaca actttgcaca
     1201 agagcggctg gacggccctg ccgcacccac cactggctgt ggatgacacg attggcgagc
     1261 tgctggaaac gcaggagcgt ttcgacaagg ccaaggccga cacgctgcgc gactgcctgc
     1321 tggcagcggg caaatacggc ctggccgggc tgccgctgcg ctacaagttt aggctggcgt
     1381 ggtgtatggt tcgctacaaa atgagctttg acgacggcgt aaagctgtac ggcaagtatg
     1441 tgggcaactg gggcggcgcg gccacccgct ggcgctttga cgccaaaaac ggcgatacgg
     1501 tagtaaaaag tgtgacgctc tgccccagcc gcaagctgca tctggaggtc accccgtcag
     1561 cccacgcctt gcgtgagggc gacacctacg acatggctgc cgtgcgggtg cgcattctgg
     1621 acgagaacgg cagccccgcc gcctacgccc agctgccgct gcaatttgcc gcaagcggcg
     1681 cggcggcgct cgtcggccca tccatcgcca cggcggaggg cggcatgggc ggcacctaca
     1741 tccgcacaat aggccagacc ggcacggcca gcctgaccgt ggccgccccg caatgtgagc
     1801 ctgtcaccgt aacattttcc gtcactggaa aggagaatac agcatggaac tgacaacaaa
     1861 gcaaaaggcc gcgtttggta tcggcgcggt gggcaaggat atggtctacg ccctgtcggc
     1921 atcctacgtt atgtactatt atcaggatgt gctggggctt tcggccacgt tcgtcggcct
     1981 gattctgatg attgcccgcg tgttcgacgc cttcaacgac ccgttcatgg gtgtgttggt
     2041 cgccaagacc cgcacgcggt gggggcggtt ccggccgtgg attttttcgg gcaccgtgct
     2101 caacgccgcg gttctctacg cgctgtttgc cgcgcccgcg ctggccgaag ccgggatgat
     2161 ggtctatttc agcgtcattt atattctgtg gggcgtcacc tacacgatga tggacattcc
     2221 ttattggtca atgatcccag ccgtgacccg aacccccgca gaccgtgaga atctttcagt
     2281 cgtcggccgc acctgcgcgg gtgtcggttc ggcgctgatt gcgatgttca cgatgttgct
     2341 cgtcggcgct ttgggcggtg acagtgagcg cgctggcttc cgctgggtgg cgctgattgt
     2401 ggccgtaatc ttcgttgtaa cggaaattct ctgctgcgct agtgtcaaag agacgagcgg
     2461 cggcgagatg aaaaccgcca ccgtcggtga gatgttcaag gcgctgtttg caaacgatca
     2521 ggcgctcgtc gtcgtgggca gcatcgtcct cattaactca gcgctgtacc tcacgtccaa
     2581 cttcatcatc tacttcttta aatatgactt tggtggaacg ggctggaaag ccagctacac
     2641 gctgttttcc accatcggcg gcgcggcgca gattctgggc atgatgatcc tttacccgct
     2701 cgtgcgcaaa aaattaagca ataccggcgt gtttacgtta agtctggcgc tggcgctggg
     2761 cggctacggt gtgctgcttg tgctctgcct ggccggcttc tcggggaatc tggtgttgct
     2821 gtgcgtgccg ggcgttgtgg tgttcgcgtg taacggtatg ctcagtgtgc tgacgacgct
     2881 gttcctctcg aacagtgtag actacggcca gctcaagacc ggccggcgcg aggagagcgt
     2941 gattttctcg atgcagacgt tcgtcgtaaa ggcggcgtcc ggcgtggcgg tgttcctgac
     3001 tggcatcggc ctggacctga tcggcctggt cggcaacacc gaggagaccg gtcccgtggc
     3061 cgcgcagagc gcctcgactc tgctgggcct gcgcctgatg atgaccgtgc tgccgatggt
     3121 cgttctggtc gcggcgctgg tgctgttccg ccgcaaattc accctgagcg acgaccacgc
     3181 ggccgagatc agcgcacagc tgcacggcaa aaaagagggt gtctgatatg acggagctac
     3241 attggcgcgc ggccacggat gcgggaagct atgacggtac agcatcgatt tcggtagggc
     3301 agggggctgt gcatctgcgc actgacctgc ctgccggaac gctggaaaat gctgtggcga
     3361 cgctgtcctg gccgatggcc gagaatgaaa aaatcttcat gaacggctac cagacctgga
     3421 ccgagtgccc ggaactgccc aagtggggcc gccagcgcgg caccgaccac attccgaaat
     3481 ttctgctgga caaatacgcc tttgaccgct acggcgacta ccattttgtg cgctacagca
     3541 aacgaaaagg tatgagccac gggttctcct actgctattt ccgcagtggg gaacactacc
     3601 gcctcgtggc aagcctcgac gaaacgcccg gctacacgat tttttactac gacgccaagg
     3661 cggctgtctt gcgcatcgag cgggactgcg ccggggtgtg ccacggcggc agtgtgtttg
     3721 cggcattcga tttattcttt actgagggca gcgaggacga ggtctttgac gcatggttcg
     3781 cggcgctcgg ctgccgcccg cgcactacaa aaaagctggc cgggtattcc agctggtata
     3841 accgctacca aaacattgac gaagcgtcaa ttttggagga cctgcagggg tgcagggcac
     3901 tgttcaaacc cggagacctc tttcaaatcg acgatggctg ggaagcaaag gtcggcgatt
     3961 ggctcgaaac cgaccccgct aaattccccc atgggctgcg cccgctggcc gacgctgcgc
     4021 acgacagcgg ctttctgacc ggactgtggt tggccccctt cgtctgcgaa aaagagtctg
     4081 ccgtttaccg cgatcatccc gactggctgc tgcaagtgaa cggccaaccc tggtgctgcg
     4141 gcagcaactg gagcagcttt tatgcactgg acatcgacaa cccggaagtg ctggactacc
     4201 tgcaaactgt gtttgaccgg gtgttgaacg actggggttt tgatttggta aagctcgact
     4261 ttttgtacgg tgcagcgacg tttggcagcg caagcgaatc ccgcgccgca cggatgcagc
     4321 gggctatagc gctgctgcgc acctggtgcg gcgacaagct gattttaggc tgcggcgtgc
     4381 cggtcatgcc cgcgttcggc gtggtagatt actgccgcat cggctgtgac gtgagcctgg
     4441 actgggacga cgtgtggtat atgcggctgt tccaccgcga gagggtgtcc acaaagcaga
     4501 gcatcggcaa cacggttttc cgccgccagc tcaatggccg cgcctacggc agcgatccgg
     4561 atgtattctt tttacgggag gaaaactgca agttgacccc gcagcaaaag gaccagcttg
     4621 ccacggtcaa cgcgttgttc agcggcattt tgctgacgtc cgacatgccc agccgttaca
     4681 ccctcgaaat gcgcgaacaa tacgcggcct tgcgcgacct atccgcccac gctgaaaata
     4741 tctgcgtcga tgctgaccac gacatctgta tacgatacac cctgcatggg caagaaaaga
     4801 cacttgtact ggaaaagaag aaattgtaaa tctgcggaaa atgtgaaaat tgcaaggttt
     4861 actatactca aatttaacaa tttaaatcga tgaagcgtag ataacggcgc actatgcccg
     4921 tacagagagc ctgtgatgct gagagtcagg ccggaaacgg agcgccaaat ggaccgccga
     4981 gggtgcagtc aaatgtgggc gaccgcaaag tattctgcaa cgtgggggca tacgtcagtt
     5041 gccggggatg tgttggcatc ccgctaagcg catgggcggc aaggcccatg aaccagggtg
     5101 gcaccgcgaa gaattttcgt ccctggcaga aagagaaatc tttctaccag ggacattttg
     5161 tttttgcaag ggggaaggga agatgaaatt ttcaccgccg gtcgaagaag tcaaggcact
     5221 cacggcccgg ggcgagtaca aggtcgcgcc catcagctgc gagatattgt cggacatctg
     5281 cacgcccatc gagtcaatgc agatactcaa aaacgtttcg acacactgct acctgctgga
     5341 atccccaggc caacgaaaag tggggccgct acacgttttt gggctatgac cccaagatgg
     5401 agataacgtg cgtgaacgga accttgcgcg ccggggacgt gaccctgcat accgacgacc
     5461 catcggcggt gctgcgggac attctggccc ggtacaaaag ccagcgcctg cccggcctgc
     5521 caacgtttac cggcggcctt gtcggctact tctcctatga ttacctcggt taccgcgaac
     5581 cgaaggcccg cactgacgct caggataccg agaactttaa agatgtagat ttgatgctgt
     5641 ttgacaaggt catcgccttt gacaatctgc gacagaaaat catcctgatt gcaaatatgc
     5701 cgctggacgc cgtacagaca aactacaaca aagccacgcg ggaattgcag cagatggccg
     5761 acctgctgcg ccacggcacc aaaaagcagg aaccgcccgg caaactgctg ggtgagttta
     5821 caccgctctt cgaccgcgct gcctactgcg caatggtcga gcgagcacgg catcatattt
     5881 acgagggcaa catcttccag atcgtgctct ctgaccggct gtccgcgccg tttgatctat
     5941 accagggaga cagcaaatat agacgaggaa agctgagcgc caaaaacttc ggtatggagg
     6001 ataacaaaaa attgtaccgt tgtacgttgc attttgcctg tgataataga accccttggc
     6061 aaaacttgta tgcgggtgcg ttggctgagt ggctgcctgc gacatttttc ttgtcatttc
     6121 aaaaaatggt agtaaaataa aggtatgctg tctgaagagc accccgtatt ctctgtgatg
     6181 cgaagatgcg gtttttgaca cacagacagc gtgacagaaa ttgaggaggg gctacgccat
     6241 ggaaatcggc caaaaagtca aaatcatctc agacagtacc tgcgatcttt cacaggagct
     6301 gacggcgaag tacgatatcg atattctgcc gctgcatatc ctgctgggcg aaaacgagtt
     6361 ccgggacggc aaagatacca cacccgatcg catttttgcg tgggcggatg caaacaaggc
     6421 aacgccgaaa acctctgcgc cgtctctgga ggatgccaaa gtgctgctgc agccgtatgt
     6481 tgaggccggc cgtgaggtga tctgcttttc gatctccggc agcatgtcca cctccggcaa
     6541 tgtcatgcgc cttgccgccg aggatctgga tgccgccgcg caggtcacga tcatcgactc
     6601 cgagaatctc tctaccggga tcggacttct tgtgttggaa gctgccatcc tggcagagca
     6661 ggggcatacg gcggcagaga tcaccgctgc cgtcaggaaa ctcatcccga aggtgcgggc
     6721 aagctttgtc gttgatacat tggtatacct gtggcgaggc gggcgatgca atgccgttgc
     6781 cgctttggca ggcagtgcgc tgcggctgca ccccaagatc gtggtggaaa acggtgccat
     6841 gcgggcaaca aagaagtacc gcgggaaaat cgacaatgtg attatggcct atgtgaagga
     6901 catggaagct gacctgaagg cagcccgacc ggagagggtc tttattaccc actccggctg
     6961 cagccgtgag gtagtggggg aagtacgctc ttatctgaaa gagcttaatg tattctgtga
     7021 gatccttgaa acaagagccg gcggcgtgat ttccagccac tgcggcccgg gcacgctcgg
     7081 ggtgctgttc atagcaaagt gatgtagcgc gcgtgctgtt gccgaattcc gaatcttaac
     7141 aacgccggca cagtaagcaa cgcagatgat caatcgtata aatacaccaa atttgaataa
     7201 ggcgtaaagg accagaagaa tggccgattc tctgatgacc tgcaccccct acggtatcaa
     7261 tacccatcgc ctgatcgtgc aagccagacg agtagcttat aacggcgagg atgacgcgcc
     7321 agccaagact gtacaagcga tcattcatta tacagtgcgg tctttcaaac gcacttacac
     7381 actgtacatc ggtgccgctg taatactctt tctggtggtg gttcttttcc gccgcggaca
     7441 gagaaaacgc aagacccata agaagtaaaa cagaaagaaa cggtcaactg ccgcaccttg
     7501 ctcgccgtat cggcacttag aattatgcgg tattgcctta gcattatgcg gcattgcccc
     7561 aaaaaaatga ggtaccccct tttagccaaa ctgcttaaag gaggtacctc attttttggg
     7621 caaaagcgcc gtacaattag atgttccgga tgcggtgtcc ggccggatgg ctgaggcgga
     7681 gactgaggtt atcgataatg ccggggcggt tgcggagaat aacgcataaa taaatacgcg
     7741 aatcggaaaa agcaaagcag cttccgcttt gctttttcta ttaatgctga atatcaatcg
     7801 gtcagaagat ccatagccct ttgggcaatc gaaatacgct gcttctcgcc gccggaaagg
     7861 cttgcgccgc cttcgccgat gaccgtgttg tagccgtcgg gcagggccgt gataaagtcg
     7921 tcgcagcggg ttttcttcgc ggcagccacg atctgcttag gggcacgtcg tagccgccaa
     7981 tcatcacgct gggcgcggcc atgtgggcgg ccctggggga gaaatccctc cggccgctta
     8041 cgccgtctac ttccctttaa caagggaggc tttaatggtg ccccccgcaa tgccctttgg
     8101 cagcctctgg gttgtgtacc agcttcccgg ccaacagggc cagcacggcc tggtcggcgc
     8161 tgccggtcgt cccggcaaac aagcggatgc ctgccccggc cagggcactg accgcgccgc
     8221 cgccgatgcc gccgcagatg accgtgttta cgccctggcg ctgcaaatag ctgaccatgg
     8281 cgctgtggcc gctggctgtg gcatccacaa cggcggtgtc taaaatcatg ccgctttcga
     8341 cggtgtacag tttaaattgt gcggcgtggc caaaatgctg gtagacctgg ccgtcagcat
     8401 aggtgactgc gattttcatg gtgattctcc tttgtcaaca aaaaagccgc atcttccgac
     8461 gcagccccaa cacttctatt ataccacgcg caccgctgcc gcgcaagggc cgaacctgca
     8521 caaatctgac gccgcaacgt tgtgaactct gcgaatggac gcacagccgg gggtgtgata
     8581 tagtatagtc acagaaagaa aacagccgga gataaggaac taaaagttcc aagtgccggt
     8641 cggatttcgg aaggaaggag gctcaagtcc aatggcaaac tttaaagatc ctattcatca
     8701 ccggcgggcc tgggtcgcct ataagtgctg aacaaaccgc atccgaagcc gcaagtcata
     8761 acttgtaaaa gattgaaaaa atatgatgca cggtaggttg gcactttgtt agagagaccc
     8821 aggggccgtc acaaaaagac ccttgacaca tatatatcag gagaggggcg gtacagatat
     8881 gaccgcagaa acaacacccg actaagcaag tggaacttta cctgatacca taggagatat
     8941 tccaatgtac tgagttaaaa gaccgcaacc aacgataaag ggtatgaggc ttatgactcc
     9001 aatcatttaa catccccggg gcgaacgaga gaagttcgcc ccggtttttt gtttgcggaa
     9061 aatatgctat aataccccca aagggggcta taccatgctg catatctact acggcgacgg
     9121 caagggcaag accaccgccg ccatggggct ggcgctgcgg gcgctgggcc atgaaaaaca
     9181 cgtgacgatt ctgcaatttt taaaggacgg ttccagcggc gaaatcgcac tgctgcgccg
     9241 ctgcggggcc gacgtccacg cctgcccgaa cgcgaaattc acctggctga tgacagccga
     9301 cgagaaggcc gaagcaaagg agcacaacaa cgctgccctg cgcaccgcgc tggccgcgcc
     9361 gtttgatctg ctggtcctcg acgaagcctg cgcggcctgc cgtaccggcc tggtggacga
     9421 gagcctgtta cagcaggccg ccgcgctggc cgggcagggg agagaggtgg ttttcaccgg
     9481 tcgcaatccc gctgcctgga tgctggaaca ggctgactac gcgaccgaga tgcgcgccga
     9541 gaaacaccca tatagtaaag gcgtggcagc gagagaaggg gttgaatttt aaagactccc
     9601 tttgacaagg acggtttttt ctttcttttg tgtactctgc acaaagaatg ctccttgctt
     9661 ttgtagctgc aaaaccgaat ttcgcagaaa aacgaccgaa tttcgcaaag tgttgcagta
     9721 gtgttactac atcgtgtaca atggcgttgt accgttaaaa ataaaaacaa ggagagaatc
     9781 atggacgaaa tcaaagccca aaagaaagcg ctgaaaaagg catacaaaaa ggccaagcgt
     9841 aagaccgtcc tggggtggaa gatcctgacg atcctctgcg ccatcctcat ggtcgttttc
     9901 attcccctgt gtaccatcct gaataccttc gacaacacag ttgcggcgtt tgtcggcggc
     9961 acgttctgga aactggaaaa cgaggatccc aacgcacagt actttaccac cgacttttcc
    10021 tcgccggaag agatggtcga ctacggtttg accgtcgccc agcaggtcga ggctgagggt
    10081 gcggccctgc tgatgaacaa cggcgcgctg cccctggcca aggacgcgaa ggtcagcacc
    10141 ttctcctcct ccagcgtcaa cctcgtctac ggcggcaccg gctccggcaa tatcgatgcc
    10201 tcgaccgccg acaccctgcg caccgcgctg gaaaaggtcg gcgtcaccgt caaccctacg
    10261 ctgtgggatt tctacgccac cggcgcaggc aaggattaca cccgcgccaa tggcggtacg
    10321 gtatccaccg ccagcgccac cacggcggaa gtgccctggg acgtctacac cgatgatgta
    10381 aagtccagcg tcgctcagta cggcgacgca gccatcgtca ctctctcccg cgtcggcggc
    10441 gagggtgcgg acctgagcta cggcgaggtc aactacctgg cactggacga caacgagaaa
    10501 tctatgctgg aaaacctcgc ctccatgaag aaaaacggcg agatcaagag catcatcgtc
    10561 ctcatcaact ccgctaacgc cttgcaggtc gacttcctga agaacaacga ctacgacgtg
    10621 gatgccgcgc tctggatcgg cgacgtgggc atctccggca tcaacgccgt tgcccagatt
    10681 ctggtcggcg acgtcaaccc ctccggcagt ctggtcgata cctactgcta tgacaattac
    10741 agcgcccccg ctatgtggaa tttcaccccc agcgtctacg acggctatgt cgagggcggc
    10801 gatgtgcctg ccaagggcag cacctacatg atctaccagg agggtatcta tgtaggctac
    10861 aagtactacg agacgcgcta tgaggattac gtcatgggcg cgggcaacgc gggcgactac
    10921 gcctacggcg acattgtggc ttaccccttc ggctatggtc tgagctacac caatttcaag
    10981 ttcagcgata tgaccgtcaa ctacaacgac gcgttggacg tttataccgt cgctgtcaag
    11041 gtcacgaaca ccggcgcagt cgcgggcaag cgcaccgtgc agctctacgt gcagagcccc
    11101 tacaccgact acgacaagca gaacggcgtc gagaaggccg ccgtcgccct ggtcggattc
    11161 ggcaagacgg gagagctggc ccccggcacc tccgagatcg ttaccatgaa tgtcaacaag
    11221 cgcgatatcg ccagctatga cacctacggc gcgggcacct acattctgga tgccggcgac
    11281 tactatttca ccgccgccaa cgacgcccac gaggccgtga acaacatcct ggctgccaaa
    11341 ggctacacgg tggcttccac caacggcaag atgaccgccg acggcgacgc cgacctgacc
    11401 tatgcgtgga ctgaggacaa gctcgacacc acgacctact ctgtcagtga gaacggcacc
    11461 gccatcacca accagctgtc ctgcgccgac ccgaatctgt acgacggcga ggacaacacc
    11521 gtgacctggc tgagccgcag cgactggaac ggtaccctgc ccaccgagac cgtcaagctg
    11581 gccctgaccg agcagctgaa gaaagacctg caggatatcc gctacgaccc cgccgactac
    11641 gaggccgtga acctgcctgc catgggcaag aacaacggcg tcaccctgta tgacatgatc
    11701 ggcctcgact acgacgaccc gaagtgggac gacctgctgg acaacctgac tttcgacgag
    11761 atgaacaccc tcattggcga tgcgttccac tggacgatgc cggtcaagag catcgaggcc
    11821 cccggcaccc gcgatgagaa cggcccccag ggcctgaccg cc
//
LOCUS       DBMDSQ010000034        11773 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_1642_length_11773_cov_2.253855, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000034 DBMDSQ010000000
VERSION     DBMDSQ010000034.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 11773)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 11773)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11773
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1642_length_11773_cov_2.253855"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1765)
                     /locus_tag="ACZPAX_02345"
     CDS             complement(<1..1765)
                     /locus_tag="ACZPAX_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007045752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HIR1112771.1"
                     /translation="MPNHAEQLAQELNLNVKNVQGAIELIDQGNTIPFIARYRKEATG
                     SMDDQVLRDLGDRLEYLRGLDKRKEEVTSSITEQGAMTPELTAALTKAKTLAEVEDIY
                     RPYKPKRKTRASIAKARGLQPLADAILKQDAAFDPEKAAAAYLNDEVPDAAAALAGAQ
                     DIIAEAVSDDAACRAELRRYYRAFGRIASAKAKDEDSVYAQYYDFAEPLNKIPGHRVL
                     ALDRGEREGFLKVGIQVDADRAAGIVSWMYARKKTGGKSAELVDAAARDAYSRLIHPS
                     LENELRSELSDAAAEGAIKVFGDNLRQLLLQPPIRGKTVMGLDPGYRMGCKVAVVDPT
                     GKVLDTNVVYPVPEFKRVDQAKKIIKAMVLKNGVEVMAIGNGTAGHETEEFAAQVIRE
                     LADEKNLHLQYMVVSEAGASVYSASKLAAEEFPQYDVNLRSAVSIARRLQDPLAELVK
                     IDPKAVGVGQYQHDMPQKRLNETLDGVVEDCVNSVGVDLNTASAPLLRRVAGVSAATA
                     KNIVAWREEEGAFTSRAQLKKVKGLGPKAYEQCAGFLRLPEAKNRLDATAVHPESYAA
                     AKALLDACGYTAAEIGTDKLAG"
     gene            complement(1749..4115)
                     /locus_tag="ACZPAX_02350"
     CDS             complement(1749..4115)
                     /locus_tag="ACZPAX_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015537530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HIR1112772.1"
                     /translation="MESVNFKQKSNYWRVFVLCFATAVLLFLPHCIMDAVAGGGYFHY
                     AGDFNDQQINFYQYANAFIKQGGTFSWATDLGSGFVNSYSFYLLGSPFFWLSMLVPAK
                     FMPWAMVPLLCLKMALAGGGAYLWARRWVQDERWSMLAGCLYAFSGFSIYNIFFNHFL
                     DVVALFPYMLASLDDAVLDGKKGRFPFWVALNLVNNYFFFAGQAVFLILYFFCMAAGR
                     RYKIGPRKFASLAWETVLGCACGCVLLLPAGLSLLQNPRTIDPFTGYGYLFYGKSQQY
                     GAIFYSPFLMPDAPYFKDMFQEGILKRTSLTAYLPLVGAAGGLAFCRARGRHPFTYIL
                     KVCIVCAFVPVLNSAFYALNSSYYARWYYMPILVLCGATCYLLARPGLAERHLLRAFA
                     LTTFITLTAAVFAILPNEDDDGNTVYGVLDQPARFWAIFGMTVAGIVVFALLWHFCRQ
                     KRRWGSILMAATLGFSLLYGTLHLSLTKYAQWDTDSNLIAETYDSVDEIAAALPDDAF
                     YRIDAYGAHNNLGLWFDRSCLQFFNSTVAPSIMEFYPEVGVKRDVNSKPEAENYALRG
                     LLSVRYTLVAKDKEAEWTDKALPGWRRTGETTVYAIYENENWVPMGFAFDYYVTADQL
                     DRVNEDERAQILCRAVLLDDDQISAFGGILQPLPDEELTNRSEDAYAADCVARRDAGV
                     AEFAATRTGFTARTNYAADELVFFSVPYDDGFTATVNGEPATIEKVDDGLMAVCVPAG
                     ENEIEFTYHTPGLKVSATVSAVAIVVYGVYLGILRKKKRGNKPYAKPC"
     gene            4210..4812
                     /gene="yfbR"
                     /locus_tag="ACZPAX_02355"
     CDS             4210..4812
                     /gene="yfbR"
                     /locus_tag="ACZPAX_02355"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007045750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002953 - 5'-deoxynucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HIR1112773.1"
                     /translation="MKTYPFSALLSRMKYITRWSLMRSTRAESLSEHTADTALLAHTL
                     CLIGQKCTATGKSLRPEVVATAALYHDAPEILTGDLPTPVKYKNDALRTAYKAVEHEG
                     ARVMASLQPAELQAETQAWLTGSLLNDAERKIVKAADRLSALIKCMEERQSGSHEFEA
                     AEAQQLAALHEMHCPEAEYFIEHMLPCFAQNLDELTRGRF"
     gene            complement(4840..5814)
                     /gene="dusB"
                     /locus_tag="ACZPAX_02360"
     CDS             complement(4840..5814)
                     /gene="dusB"
                     /locus_tag="ACZPAX_02360"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007045749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HIR1112774.1"
                     /translation="MQLRNLQIPTGAVFAPMAGLTDAACRHLMADHGAAWTVSEMASA
                     KALNFGDRKSLELLKDEHPHGLYAIQLFGAEPESMAKAILFVREKGIRFDILDINMGC
                     PAPKITSSGAGSKLLLDPPLCGQMAAAARKALGDDTPLTVKMRIGWDADNLTGVEVAK
                     QLEAGGADLIAVHGRTREQMYIPPIDTAAIAAIKQAVSIPVLANGDVTSADGALTLLK
                     ETGCDGVMIGRGALGDPWLFAQVRAALLGEERPPEPTLNQRMAALRAQIYEMCEEKGE
                     WAAMPQARSQAMHYMKGLHGAAALRRYCSMLEHFTDVDRLIDAVYKLQ"
     gene            5936..8458
                     /gene="polA"
                     /locus_tag="ACZPAX_02365"
     CDS             5936..8458
                     /gene="polA"
                     /locus_tag="ACZPAX_02365"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015536800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HIR1112775.1"
                     /translation="MKLMVLDGNSLVNRAFFGIKLLTTKDGRYTNAIYGFQNILLNLL
                     AAHKPDAVAIAWDERAPTFRHNAYDGYKATRHGMPEELAQQMPVLKELLTDLGFVQVS
                     KAGWEADDILGTLAAACEAAGGTTLLATGDRDSLQLVDDATTVLLATNKETLPMDPTA
                     IREKYGIEPPQLIDVKSLMGDASDNIPGVPGIGEKTALALISKFGSLQGVYDNIEDKA
                     VKPGQRAKLTANRDKADLSYMLGTIRKDAPIESDPAAYVRQPGDPAAAAQLLAALEMH
                     KMVARWGLDEGAAPAAADTAPLETVESSPLPLLLEGRFYAAQSASKEGGSAWYLVQGK
                     DVYLPDADRLAAILDGDAEIWAFDAKPLYRLALEHGGIGKALRFDGKLAAYLLNPSAS
                     GYEVHSLAAEYGVHADFTCEAAPDAGVLAGLCDALAAALDESGQRKLLDEMELPLARV
                     LADMERIGFAVDADGIRAFGDSLRGELDGILNNIYTEVGYEFNVNSPKQLGEALFDKL
                     GLPPRKKNARGYSTDAETLESLRPYSPVIDEILKYRTYSKLLSTYVDGLLNATATDGR
                     VHSTFIQTEARTGRISSTEPNLQNIPIRTELGSRLRGYFVAGQGETLVDADYSQIELR
                     ILAHITGDEHMQQAFLNGADIHRSTAAKIYHIPESEVTPQLRSASKAINFGIMYGKGA
                     FSLGKDLGISVKEADAFLKTYLDTFPKVDGYMKDCIAHAKDKGYVETLFGRRRALPEL
                     ASSNFQVRASGERMARNTPIQGTAADIIKLAMVHVWQRLRDEKLQARLLLQVHDELIV
                     EAPDAEINEVKRILKEEMENVVHYSVPLTTEVGTGKTWLAAH"
     gene            8592..9599
                     /gene="tsaD"
                     /locus_tag="ACZPAX_02370"
     CDS             8592..9599
                     /gene="tsaD"
                     /locus_tag="ACZPAX_02370"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005945426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HIR1112776.1"
                     /translation="MYVLGIESTCDETAAAIVENGRKVISNIISTSVREQALYGGVVP
                     EIASRRHAEYISATVDKALADANMTIADVDAVAVTFAPGLIGAVLVGVNFAKGLAYAA
                     NKPLVPVHHLRGHIAANYLTHQDLKPPFLCLVASGGHSHIVLVEDWCRYKILGRTVDD
                     AAGEAYDKVARTLGLPYPGGPSVAAAARDGNPKAYKLPVPHVDGKYNVSFSGLKTAVI
                     NEVHNAEQKGQAVNVADMAASFQERIDQILAKKLLAAAADTNAQQVVLAGGVAANGRL
                     RQLVNDGAQKLGAKVYLPELKYCGDNGAMIAAQGYYEYQDGNLADWTLNGLPTLPIDY
                     R"
     gene            complement(9644..10612)
                     /locus_tag="ACZPAX_02375"
     CDS             complement(9644..10612)
                     /locus_tag="ACZPAX_02375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1112777.1"
                     /translation="MNEAQWQNRMTMLVNSCDAYEDLWQPFFTLLKRYFAPLDMRILL
                     NTESKDFRFEGLNIECVHSSAATYGERMTDALRRVKTEYTLLMLDDFFLREPVKMDRL
                     HDLVRWMDADRDIVYFSSDITEALYDWEVDRYPGYRRLPVGNRYTLNMQAAVWRTDKF
                     AEYWNHKVSPWDWEERCNVLTAAHPKDKFYCVLNEEARFLNYGYHKGQWMGICHGKWV
                     EHDVVPFFAKEGIEIDYAKRGFVDLAHRPASLQKETARSERYDLVRRCLGAGYLLPYF
                     LFCRRCNLYSKKNHCAVDEDYFHYLQRKADLQSQTGKKCLFGPMQR"
     gene            complement(10615..11448)
                     /locus_tag="ACZPAX_02380"
     CDS             complement(10615..11448)
                     /locus_tag="ACZPAX_02380"
                     /inference="COORDINATES: protein motif:HMM:NF041524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 6 glucosyltransferase"
                     /protein_id="HIR1112778.1"
                     /translation="MMTKVAALYIATGKYTVFWPEFYESAEQYLLRDCEVHYFVFTDA
                     DALPGEENPRVHICPQEAYSWPFATLRRFEIFLKQEQALRGFDYIFFFNANAQFMQPV
                     TREMLLPRAEKGERLLVVQHPSFYAKPNYEFTYDRNPRSTACIPYGLGKYYVCGGVNG
                     GMAPAFLELCRTLDKRIRRDLQHNVIALWHDESQINRYILFRHDFRVLDPSFCYPEGW
                     DHLPFPCVVRIRSKARYLDILALRKDAPETQLSPAVQRWNHFAMRAARWSQNHLFKKG
                     K"
     gene            complement(11445..>11773)
                     /locus_tag="ACZPAX_02385"
     CDS             complement(11445..>11773)
                     /locus_tag="ACZPAX_02385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1112779.1"
                     /translation="ENIGLRFEVRDEVGTPLATSSMFGLTLQPGINEFTAEYDLSVLT
                     VGKYSNYFTVITSYGGGTYGVEDWVPGIQFQLVDTFATYYEGWNRRAWGPICLPEPAV
                     TLGAEE"
BASE COUNT         2363 a         3661 c         3565 g         2184 t
ORIGIN      
        1 ggcccgccag cttgtcggtg ccgatctcgg cggcggtgta gccgcaggca tccagcaggg
       61 ccttggcggc ggcgtagctc tcggggtgga cggcggtcgc gtccaggcgg ttcttcgcct
      121 cgggcagccg caggaagccc gcgcactgct catacgcctt ggggccgagg cccttgacct
      181 ttttcagctg cgcacgggag gtgaacgcgc cctcctcctc gcgccaggca acgatattct
      241 tggccgtggc cgcgctgacg cccgccacgc ggcgcagcag cggcgcagag gcagtgttca
      301 ggtcgacgcc gacactgttc acgcagtcct cgaccacgcc gtccagcgtc tcgttcagcc
      361 gcttctgggg catatcgtgc tggtactggc ccacgccgac ggccttgggg tcgattttga
      421 ccaactcggc cagcggatcc tgcaaacggc gggcaatgga cacagcgctg cgcaggttga
      481 cgtcatactg ggggaattcc tcggcggcca gtttgctggc ggagtagacc gacgcgccgg
      541 cctcgctgac gaccatatac tgcaaatgca ggttcttttc gtcggccagc tcacggatga
      601 cctgggcggc gaattcctct gtctcgtggc cggccgtgcc gttgccgatg gccatgacct
      661 cgacgccgtt cttcaaaacc atcgctttga taattttttt cgcctgatcc acgcgcttga
      721 actcgggcac cgggtagacg acgttcgtgt ccagcacctt gccggtgggg tccacgacgg
      781 cgaccttgca gcccatgcgg tagccggggt ccaggcccat aaccgtcttg ccgcggatgg
      841 gcggctgtaa aagcagctgg cgaaggttat cgccaaagac cttgatagca ccctcggcgg
      901 cggcatcgga cagctcgctc cgcagttcgt tttccaggct ggggtggatg aggcggctgt
      961 atgcgtcgcg ggcggcagcg tccacgagtt ccgcgctctt gccgccggtc tttttgcggg
     1021 cgtacatcca gctgacgata cccgccgcac ggtcggcatc gacctgaatg ccaaccttca
     1081 aaaagccctc gcgctcgccg cggtccagtg ccagcacacg gtggccgggt atcttgttca
     1141 gcggctctgc gaagtcgtaa tactgggcgt agacgctgtc ctcgtccttg gccttggcgc
     1201 tggcgatgcg gccgaacgca cggtagtagc ggcgcagttc ggcgcggcag gcagcatcat
     1261 cggagacagc ctccgcgatg atatcctgcg ccccggccag cgcggcggct gcgtcgggga
     1321 cctcgtcgtt gaggtacgca gcggcggctt tttcggggtc aaacgcagcg tcctgtttca
     1381 gaatcgcgtc ggccaacggc tgcaagcccc gggccttggc gatgctggcg cgggtcttgc
     1441 gcttgggctt ataggggcgg tagatgtcct cgacctcggc caaggtcttg gccttggtca
     1501 gcgcggcggt caattcgggc gtcatggccc cctgctcggt gatggaggag gtgacttcct
     1561 ccttgcgctt atcgaggccg cgcagatatt ccagacggtc gcccagatcg cgcagtacct
     1621 gatcgtccat cgagccggtg gcctccttgc ggtagcgggc gataaagggg atggtgttcc
     1681 cctggtcaat caattcaata gccccctgga catttttgac attcaggttc aattcctggg
     1741 ctaactgttc agcatggttt ggcatatggt ttgtttcctc tctttttctt ccgcaaaatt
     1801 cccaaataca ctccatacac cacgatagca acggcgctca cggtggccga caccttcagc
     1861 cccggcgtat ggtaggtaaa ttctatctca ttctcccctg cgggcacgca cacggccatc
     1921 aggccgtcgt ccactttttc aatggtggcg ggttcgccgt tgactgtggc ggtaaagccg
     1981 tcgtcatacg gcacactgaa aaacaccagc tcgtccgcgg catagttcgt cctggccgta
     2041 aaaccggtgc gggtcgcggc aaattctgcc acacctgcat cccgccgggc gacgcagtcg
     2101 gcagcgtagg catcctcact gcggttggtc agctcctcat cgggcagagg ctgcaaaata
     2161 ccgccgaacg ccgaaatctg gtcgtcatcc agcaaaaccg cgcggcacaa aatttgcgca
     2221 cgctcgtcct cgttcacgcg gtcgagctgg tcggcggtga cgtagtagtc gaatgcaaag
     2281 cccatcggca cccagttttc gttctcgtaa atggcgtaga ccgtcgtctc gccggtgcgc
     2341 cgccagccgg gcagagcctt gtcggtccac tcagcctcct tgtccttggc taccagcgtg
     2401 taccgcacgc tgagcagccc gcgcagtgcg tagttctcgg cctcgggctt ggagttcacg
     2461 tcccgcttga cgccgacttc tgggtaaaat tccatgatgc tgggcgcgac ggtggagttg
     2521 aaaaattgca gacaactcct atcgaaccac agccccagat tgttgtgcgc cccataggca
     2581 tcgatgcgat aaaacgcgtc gtccggcagg gcggcggcaa tctcatccac agaatcatac
     2641 gtctctgcga tcaaattgct atcggtgtcc cactgggcgt acttggtcag cgagaggtgc
     2701 agcgtaccgt acagcaggct gaaccccagc gtcgccgcca tcagaatact gccccagcgg
     2761 cgcttttggc ggcaaaaatg ccacagcaac gcaaaaacca cgatacctgc caccgtcatg
     2821 ccgaagatgg cccagaaccg cgcaggctga tccaacacgc cgtagaccgt gttgccgtcg
     2881 tcatcctcgt tgggcaggat ggcgaatacc gccgccgtca gcgtgataaa ggtcgtcagc
     2941 gcgaacgccc ggagcaggtg ccgctccgcc aggccggggc gggcaagcag atagcacgtc
     3001 gcaccgcaca aaaccagaat cggcatataa taccagcggg catagtagct ggaattgagg
     3061 gcgtagaacg cactgttcag caccggcacg aacgcgcaga cgatgcacac tttcagaatg
     3121 taggtaaacg ggtgccgccc ccgcgcccgg cagaacgcca acccgcccgc cgcacccacc
     3181 agcggcaggt aggcggtcag gctcgtgcgt ttgaggatgc cttcctggaa catatccttg
     3241 aaatagggcg catccggcat caaaaagggg ctgtaaaaaa tggcaccgta ctgctgggat
     3301 ttgccgtaga acaagtatcc atacccggta aacgggtcga tggtgcgcgg attttgcaaa
     3361 agcgacagcc ccgccggaag cagcagcacg cacccgcagg cgcagcccag gaccgtctcc
     3421 cacgcaaggc ttgcaaactt gcgcgggccg attttatacc gccgccccgc cgccatgcag
     3481 aagaaataga ggatgaggaa cacggcctgc ccggcaaaaa agaagtagtt gttgacaagg
     3541 ttcagcgcca cccagaacgg aaagcggcct tttttgccgt ccagcaccgc gtcgtccagc
     3601 gacgccagca tatagggaaa cagcgccaca acgtccaaaa agtggttgaa aaagatgttg
     3661 tagatgctga acccggagaa tgcgtacaga cagcccgcca gcatggacca tctttcgtcc
     3721 tgcacccagc ggcgtgccca caggtaagcc ccgcccccgg ccagcgccat tttcaggcag
     3781 agcagcggca ccatggccca gggcatgaac ttggcgggca cgagcatcga aagccagaag
     3841 aacggactgc ccagcaggta gaacgagtag gagttgacga acccgctgcc taaatcggta
     3901 gcccagctga aggtgccgcc ctgcttaata aaggcgttgg catactggta aaagttgatc
     3961 tgctggtcgt tgaagtcgcc cgcgtagtgg aagtacccgc cgcccgcgac ggcatccatg
     4021 atgcagtggg gcagaaacag cagcaccgcc gtggcaaagc agagcacgaa cacccgccag
     4081 taattggatt tttgtttaaa atttacggat tccatattga tagtaccgtt tatggggccc
     4141 cattttgggg caaggtgtgg tatactgagg atacgcccca ttataccaca aaccggaggt
     4201 caagtgaaga tgaaaaccta tccgttcagt gcgctgctca gccgcatgaa atacatcacc
     4261 cgctggagcc tgatgcgaag cacccgggcc gaatccctga gcgagcacac cgccgacacg
     4321 gcgctgctgg cgcacaccct ctgcctgatt ggccaaaaat gcacggccac aggaaaatcc
     4381 ctgcggcccg aggtcgtcgc cacggcggcg ctttaccacg atgcaccgga gattttaacc
     4441 ggcgacctgc ccaccccggt caagtacaaa aacgacgcgc ttcgcaccgc ctacaaggcc
     4501 gtggaacacg agggggcacg ggtcatggcg agcttgcagc ccgccgaatt gcaggccgaa
     4561 acgcaggcct ggctgaccgg cagtctgctg aacgatgccg agcgcaaaat tgtaaaagca
     4621 gcggaccggc tttcggcgct catcaagtgc atggaggagc gccagagcgg cagccacgag
     4681 tttgaggccg ccgaggccca gcagctggcc gccctgcacg aaatgcactg ccccgaggcc
     4741 gaatacttca tcgagcatat gctgccctgc tttgcgcaga atctcgacga attgacgcgc
     4801 gggagatttt aaaggggccg ggcatacccg gcccctacat tattgcaatt tgtacaccgc
     4861 gtcgatcagc cggtccacat cggtaaaatg ctccagcatc gagcaatagc ggcgcagggc
     4921 ggcggcaccg tgcaggcctt tcatatagtg catggcctgg ctgcgggcct ggggcatggc
     4981 ggcccactcg cccttttcct cgcacatttc gtagatctgc gcacgcaggg cggccatccg
     5041 ctggttcagc gtcggctcgg gcgggcgttc ctcgcccagc agcgccgcgc gcacctgtgc
     5101 gaacagccag gggtcgccca aggcgccgcg cccgatcatg acaccgtcgc agccggtctc
     5161 ttttaataag gtaagtgcac cgtctgcgct ggtcacgtcg ccgttcgcca gcaccgggat
     5221 ggacaccgcc tgcttgatgg ccgcgatggc tgccgtatcg atgggcggga tgtacatttg
     5281 ctcccgcgtg cggccatgca cggcaatcaa atccgcgccg cccgcttcca gctgcttggc
     5341 gacctcgacg ccggtcaggt tgtcggcatc ccagccgatg cgcattttca cggtcagcgg
     5401 ggtgtcgtcg cccagcgcct tgcgggcggc ggctgccatc tgcccgcaga gcggcgggtc
     5461 aagcaggagc ttactgcccg cgccgctgct ggtgatcttg ggcgcggggc agcccatatt
     5521 gatatcaagg atgtcgaacc ggatgccttt ttcccggaca aacagaattg cctttgccat
     5581 ggactccggc tctgcgccga acagctggat ggcatacagg ccgtgggggt gctcatcctt
     5641 gagcagttcc agcgatttgc ggtcgccgaa attcagcgcc ttggcgctgg ccatctcgct
     5701 gacggtccag gccgcaccgt ggtcggccat caaatggcgg cacgccgcgt cggtcagccc
     5761 cgccatcggc gcaaacaccg ccccggtggg gatttgtagg ttgcgtagct gcatagttcc
     5821 tccttaaaaa ggcttccccc aagggggaag ccttttttat tttgtaatct attctaccat
     5881 attttccgca aatgtggtat actgaaatgt agattgttta taaggagtat tgcgcatgaa
     5941 actcatggtc ttggacggca acagcctggt caaccgcgcg tttttcggca tcaagctgtt
     6001 gacgacgaag gatggccgct acaccaacgc catctacggc ttccagaata tcctgctcaa
     6061 cctgctggcg gcccacaaac cggacgctgt cgccatcgcc tgggacgagc gcgcgcccac
     6121 cttccgtcac aacgcctacg acggctacaa ggccacccgc cacggtatgc ccgaggaact
     6181 ggcccagcag atgccggtgc tgaaggaact tctgactgat ctgggctttg tgcaggtcag
     6241 caaggccggc tgggaggccg acgatatttt aggcacgctg gccgccgcct gtgaggctgc
     6301 gggcggtaca acgctgctgg cgaccggcga ccgggacagt ttgcaattag tggacgatgc
     6361 cacgactgtt ttgctggcga cgaacaagga gacccttccc atggacccca ccgccatccg
     6421 cgagaaatac ggcatcgagc cgccgcagct cattgacgtc aagagcctga tgggcgatgc
     6481 ttcggacaac atccccggcg tgcccggcat cggcgagaaa accgcactgg cgctgatctc
     6541 caaattcggc agcctgcagg gcgtgtatga caacatcgag gacaaggccg tcaagcccgg
     6601 ccagcgggcc aagctgaccg ccaaccgcga caaggccgac ctttcctata tgctgggcac
     6661 gatccgcaag gacgcaccga tcgaatccga ccccgccgcc tatgtccgcc aacccggcga
     6721 cccggctgcc gccgcgcagc tgctggccgc gctggaaatg cacaagatgg tggcccgctg
     6781 gggcctggat gagggcgcag cccccgccgc tgcggacacc gccccgctgg aaacagtgga
     6841 gtcgtccccc ctgccgctgc tgttggaggg ccgcttctac gccgcccaga gcgcgagcaa
     6901 agagggcggc agcgcgtggt atctcgtgca gggcaaggac gtctacctgc ccgacgcgga
     6961 ccggctggcc gccatcctcg acggcgacgc cgagatctgg gcctttgacg caaagcccct
     7021 ctaccggctg gcactggaac atggcggcat cggtaaggcg ctgcggtttg acggcaaact
     7081 ggctgcctac ctgttgaacc cctcggccag tgggtacgag gtccactccc tggctgccga
     7141 gtacggcgta cacgccgact tcacctgcga ggctgccccc gacgcgggcg tgctggcggg
     7201 cctgtgtgat gccctggccg ccgcgctgga cgagagcggc cagcgcaaac tgctggacga
     7261 gatggagctg ccgctggccc gcgtgctggc tgacatggag cgcatcggct ttgcagtgga
     7321 cgccgacggc atccgcgcct ttggcgacag cctgcggggc gagctggacg gcattttaaa
     7381 caatatctat acagaagttg gctatgagtt caacgtaaac agtccgaagc agctgggcga
     7441 ggccctgttt gacaagctgg gcctgccgcc gcgcaagaaa aacgcccgcg gctattccac
     7501 cgacgccgag acgctggaaa gcctgcggcc gtacagcccg gtcatcgacg agattttgaa
     7561 atatcgcacc tactccaagc tgctgtccac ctacgtggac ggcctgctga acgccacggc
     7621 tacggatggg cgtgtacact cgacctttat ccagaccgag gcccgcaccg ggcgcatttc
     7681 gtccaccgag ccaaacttgc agaacatccc catccgcacc gagctgggca gccggttacg
     7741 cggctacttt gtggctgggc agggcgagac actggtggac gccgactact cccagatcga
     7801 gctgcgcatc ctcgcgcaca tcaccggtga cgagcatatg cagcaggcgt tcttgaacgg
     7861 cgcagacatc caccgctcga ctgccgccaa aatctaccac atccctgaga gcgaggtcac
     7921 gccgcagctg cgttcggcca gcaaggccat caactttggc atcatgtacg gcaagggcgc
     7981 gttcagtctg ggcaaggatc tgggcatttc ggtcaaggag gccgatgcgt tcctaaagac
     8041 ctaccttgac accttcccga aggtggacgg ttatatgaag gactgcatcg cccacgccaa
     8101 ggacaagggc tatgtggaga cgctgtttgg ccgccgccgc gctttgccgg agctggcaag
     8161 ctccaacttc caggtgcgtg ccagcggtga gcggatggca cgcaacacgc ccatccaggg
     8221 cacggcggcg gatatcatca agctggcgat ggtccatgtc tggcagcgcc tgcgggatga
     8281 aaagctgcag gcccggctgc ttttgcaggt acacgatgag ctgatcgtcg aagcgcccga
     8341 tgccgaaatt aacgaagtaa agcgcatttt gaaagaagaa atggaaaacg ttgtgcatta
     8401 cagcgtcccc ctgacgaccg aggtaggcac cggcaagaca tggcttgctg cccattaaag
     8461 cagcggcacg accgtagggg ccgaaaaagt gcggtcctcc acccgacgga ggccaccgct
     8521 ttccgggaaa atcgcaatca agaaaacgtt gagggccggg tatgcccggc ccctgcatag
     8581 aggttttgtg tatgtatgtt ttagggatcg agtccacctg cgacgagacg gcggcggcga
     8641 tcgtggagaa tggccgaaag gtcatttcca acatcatctc caccagcgtc agggagcagg
     8701 cactctacgg cggcgttgtg ccggagattg ccagccgccg ccatgcggaa tatatcagcg
     8761 ccaccgttga caaggcgctg gccgacgcca atatgaccat tgccgatgtg gacgctgtag
     8821 ccgtgacctt tgcgccgggg ctgatcggcg cggtactcgt cggcgtcaac tttgccaagg
     8881 ggctggccta tgcggcaaac aagccgctgg tgccggtgca tcacctgcgc gggcatatcg
     8941 ccgccaacta tctgacccac caggatctga aaccgccgtt tctctgcctg gtcgccagcg
     9001 gcgggcacag ccatattgtg ctggtggagg attggtgccg ctacaaaatc ttaggccgca
     9061 ccgtagacga cgccgcaggc gaagcctacg acaaggtagc ccgcacgctg ggtctgccct
     9121 accccggtgg tcccagcgtt gccgccgccg cgcgggacgg caaccccaag gcctacaaac
     9181 tgccggtgcc ccatgtggac ggcaagtaca acgtcagctt cagcggcctg aaaaccgccg
     9241 tcatcaacga agtgcataac gccgagcaga agggccaggc cgtcaacgta gccgacatgg
     9301 ccgccagttt ccaggagcgt attgaccaga ttttggcgaa aaagctactg gccgcagccg
     9361 ccgacaccaa cgcccaacag gtagttttag cgggcggcgt agccgccaac ggtcgtctgc
     9421 gccagctggt gaacgatggt gcccaaaagc tgggcgcaaa agtctatctg cccgagctga
     9481 aatactgcgg cgataacggc gccatgatcg ccgcccaggg ctactacgag taccaggacg
     9541 gcaacctggc cgactggacc ctcaacggtc tgccgacatt gccgatcgat taccggtaat
     9601 ccgtacaaaa aataccgcac agcaaaatgc tgtgcggtat tttttatcgc tgcatcgggc
     9661 caaacaggca ctttttaccg gtctggctct gcaaatcagc cttgcgttgc aaatagtgga
     9721 agtaatcctc atccacggcg caatggttct ttttgctgta caggttgcac cggcggcaga
     9781 agagaaaata cggcagcaga taccccgccc ccaggcagcg gcgcacaagg tcgtaccgct
     9841 cgctgcgggc ggtttctttt tgcaggctgg ccgggcggtg ggccaaatca acaaagccgc
     9901 gcttcgcgta gtcgatttcg atgccctctt tggcaaagaa cgggacaacg tcatgctcga
     9961 cccacttgcc gtggcaaatg cccatccact ggcccttgtg gtagccgtag ttcaaaaagc
    10021 gcgcttcctc gttgaggacg cagtagaact tatccttcgg gtgggcggcg gtcagcacat
    10081 tgcagcgctc ctcccagtcc caggggctta ccttgtggtt ccagtactca gcaaatttat
    10141 cagtgcgcca gaccgccgcc tgcatattca gcgtgtagcg gttgcccacc ggcaggcggc
    10201 ggtagccggg gtaacggtcc acttcccaat cgtagagtgc ctcggtgata tcgctgctga
    10261 aatagacgat atcccggtcc gcatccatcc agcgcaccag gtcatgcagg cggtccatct
    10321 tcaccggctc ccgcaggaag aagtcatcca gcatcagaag agtgtactcg gtcttgaccc
    10381 ggcgcagcgc gtcggtcatg cgctcaccgt aggtggcggc gctgctgtgt acacactcga
    10441 tattcagccc ctcaaagcgg aagtccttgc tctcggtgtt gagcagaatg cgcatatcca
    10501 gcggcgcaaa ataccgtttc agcagcgtaa aaaacggctg ccacagatcc tcgtaggcgt
    10561 cgcagctgtt gaccagcatg gtcatgcggt tctgccattg tgcttcgttc atcgtcattt
    10621 tccttttttg aaaaggtggt tttggctcca gcgggccgcg cgcatggcaa agtggttcca
    10681 ccgctgcact gccggggaaa gctgtgtctc cggcgcatct ttgcgcaggg ccaggatatc
    10741 cagataccgc gccttgctgc ggatgcgcac cacgcagggg aacggcagat ggtcccatcc
    10801 ttccgggtag cagaaggacg gatccagcac acggaagtca tggcggaaca ggatgtagcg
    10861 gttgatctgg gattcatcgt gccacagtgc aatgacgttg tgctgcaagt cccggcgaat
    10921 gcgcttgtcc agcgtgcggc acagctccaa aaacgccggg gccatgccgc cgttgacgcc
    10981 gccgcagacg tagtattttc ccagtccgta ggggatgcag gccgtgctgc gcgggttgcg
    11041 gtcgtaggtg aactcatagt tcggctttgc gtagaaggag ggatgctgca caacgagcag
    11101 ccgttcccct ttttcggccc ggggcagcag catttcccgc gtgacaggct gcataaactg
    11161 cgcgttggcg ttgaaaaaga agatgtagtc aaagccgcgc agagcctgct cctgcttcaa
    11221 gaaaatctca aatcggcgca gtgtggcaaa cggccaggag taggcctcct gcgggcagat
    11281 atgcacgcgg gggttctcct cccccggcag agcgtccgca tcggtgaaca caaaatagtg
    11341 tacctcgcag tcgcgcagca ggtattgctc cgcgctctcg taaaattccg gccagaacac
    11401 cgtatacttg ccggtggcaa tatacagcgc cgcaaccttc gtcatcattc ttccgccccc
    11461 agggtgacag cgggttcggg caggcagatg gggccccagg cgcggcggtt ccagccctcg
    11521 taataggtgg caaaggtgtc caccagttgg aactggatgc cggggaccca atcctcgacg
    11581 ccgtaggtgc cgccgccgta ggaggtaatg accgtgaagt agttgctgta cttgcccacg
    11641 gtcagcacgc tcaggtcata ctccgccgta aactcgttga tgccgggctg cagggtcagg
    11701 ccgaacatgc tggacgtcgc cagcggggtg ccgacttcgt cgcgcacctc aaaccgcagg
    11761 ccgatgttct cgg
//
LOCUS       DBMDSQ010000035        11269 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_1720_length_11269_cov_1.744448, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000035 DBMDSQ010000000
VERSION     DBMDSQ010000035.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 11269)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 11269)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11269
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1720_length_11269_cov_1.744448"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            576..809
                     /locus_tag="ACZPAX_02390"
     CDS             576..809
                     /locus_tag="ACZPAX_02390"
                     /inference="COORDINATES: protein motif:HMM:NF024835.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1112780.1"
                     /translation="MTNFVLLRQQMIAHDFQMKDIAEAAGLSRSAFSARIHRRADFTT
                     GEMLRIGKVLGLQPEDYYNVFMAEADTQTGARA"
     gene            806..2815
                     /locus_tag="ACZPAX_02395"
     CDS             806..2815
                     /locus_tag="ACZPAX_02395"
                     /inference="COORDINATES: protein motif:HMM:NF024872.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR1112781.1"
                     /translation="MSTGPQVAINYDNIPPEMQARKQWVLWGVDADNQKAPCQIVNPA
                     RGAKANNPLTWGTFEAARCSVELLGEAQGIGYEITPADGLVAIDFDHCIDPDGQLMEP
                     FATWKEALPGYWELSQSGHGLHGFVRGTLPAGCGNKRQIDPQDKADEREVELYIKERY
                     IALTGALWGAQTPLAEAPDAQAGVDVILGQMHMRPEDVQAEAALPDVPDRPAAEITAI
                     IAAALAKSPDFYTLWNGAEHPGKHGDESKDDMAFVCSAWKATEYQLSAGDLMHGLDAS
                     PWAQTKTNDAEKDHWDKWFACGARKDGAYRRNTALAAYQRAKQEEAEQLDGFAALAAN
                     LPAGGVFTAAELDKMDLPPVQWAVPGLLPAGLSFLVAAPKMGKSWLALDLCLSVASGG
                     EWLGRKVNQGPTLYLDLEDSANRAQARMRTLLDGFTAAPETCTFRLLAPILGPELFKI
                     LDEWVAANPGAKVVCIDTFQKIKPSAAKNETSYSADYRICAPLQSWAQQHNICVLLVH
                     HTNKNKGSTDIFESVNGSQGLTGTADAVLLLTKSEGRFAEDATLSVTGRDVDMERYLA
                     RFDKDTCHWELLGTVDEQARQSFENSPAVLTVKELTVGGKWQGTVQELADEILERYPD
                     TELPESAQGLRSYFAKVWPALKCAGIKHTTHRGAKQRTHTLSRAR"
     gene            3067..3450
                     /locus_tag="ACZPAX_02400"
     CDS             3067..3450
                     /locus_tag="ACZPAX_02400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1112782.1"
                     /translation="MPNQIYRLFLPVATWRTRRGEVELQRELCCLPRSENDLRRLINT
                     GQAAPAVRGSYCVQFGGGGHYFNDHRAALAYLCTRWGGEFIMRHLDDAVERAAHRRVI
                     AIFEARLEAEKWQNRANPGGGDTQC"
     gene            3447..3797
                     /locus_tag="ACZPAX_02405"
     CDS             3447..3797
                     /locus_tag="ACZPAX_02405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1112783.1"
                     /translation="MTPNQRRALAALLTCKSRAEASRQCGLGLRTIQDYEKQPEFADA
                     LERGRRELLADAAHRMAAGYSDTVDTLQEIVENPDTPDASRVAAARALLDYGLKFAEI
                     TDINKRLDKLEGLM"
     gene            3794..4036
                     /locus_tag="ACZPAX_02410"
     CDS             3794..4036
                     /locus_tag="ACZPAX_02410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1112784.1"
                     /translation="MKDALARLEALERGQPGDLLLIVTERQTPEGPRYELPSGDLLPL
                     PDVYAYMRLHGQGVLLIDDLPELPKPKRGRKPKHEQ"
     gene            4026..4283
                     /locus_tag="ACZPAX_02415"
     CDS             4026..4283
                     /locus_tag="ACZPAX_02415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1112785.1"
                     /translation="MSSKYLLESLHRQTATGLTVGVEDSFLHNTVRLVYRARPDGVIT
                     SWQVEGPDLPAPAVFKTEAQARAFIRGRWGIYPHTKKWGEP"
     gene            4411..5085
                     /locus_tag="ACZPAX_02420"
     CDS             4411..5085
                     /locus_tag="ACZPAX_02420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1112786.1"
                     /translation="MNYVQEARKIITDYFTAMKPSEQLLKEAADELRQGHITEAYAKE
                     LANHATNEKRTLRDNAWSQLEALLRKFALAAGLADAPNGDALQGGDYKLLADNFPMSA
                     EEFAALCERNKNNPTMLRKAMEYGNKNGGLAPYAKKYYRSAHDRVQLFKTLVQRCGAV
                     LDAEPTSPTRGDAYWNMIARDVEPWATLRAPGRANAWNWTNYLHRWRTPARPLCACAA
                     EPRRVS"
     gene            complement(5320..5562)
                     /locus_tag="ACZPAX_02425"
     CDS             complement(5320..5562)
                     /locus_tag="ACZPAX_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1112787.1"
                     /translation="MGGKTSAASKNKYIAKAYDRVNLILRKDAPQNKAAVQAAADAAG
                     ESLNAYIVGAVAQRMDREQPTEGAGTLPPEAEEMLK"
     gene            5766..5933
                     /locus_tag="ACZPAX_02430"
     CDS             5766..5933
                     /locus_tag="ACZPAX_02430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1112788.1"
                     /translation="MTSYEVTYEMQNGEHDTINVASTNLIKAAQFAENFLSVRVKGYS
                     CLVEVKEHIYD"
     gene            5933..5997
                     /locus_tag="ACZPAX_02435"
     ncRNA           5933..5997
                     /ncRNA_class="hammerhead_ribozyme"
                     /locus_tag="ACZPAX_02435"
                     /product="hammerhead ribozyme type II"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF02276"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02276"
     gene            6074..6145
                     /locus_tag="ACZPAX_02440"
     ncRNA           6074..6145
                     /ncRNA_class="hammerhead_ribozyme"
                     /locus_tag="ACZPAX_02440"
                     /product="hammerhead ribozyme type II"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF02276"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02276"
     gene            6163..6405
                     /locus_tag="ACZPAX_02445"
     CDS             6163..6405
                     /locus_tag="ACZPAX_02445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1112789.1"
                     /translation="MLDKFEPWTTQAEALRTRHVIQVSPKTHDFLDQLMDVYQARPLN
                     PASRVALLDLLTVVFLAGEISGKRKDRARRKGGERQ"
     gene            6402..6536
                     /locus_tag="ACZPAX_02450"
     CDS             6402..6536
                     /locus_tag="ACZPAX_02450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1112790.1"
                     /translation="MNITDLTEREKAILAEFRSMTEEQQDTCLRDLQELLKKKEAEPQ
                     "
     gene            6533..7603
                     /locus_tag="ACZPAX_02455"
     CDS             6533..7603
                     /locus_tag="ACZPAX_02455"
                     /inference="COORDINATES: protein motif:HMM:NF012798.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIR1112791.1"
                     /translation="MTMRTNSAKWTGTRWRIDVQKDGTRKSFYSSKPGRTGQREANAK
                     ADKWLTTGVSDTALRVSQAYAMWYETQQETTSEGNCRNIATRWRHWIEPNIGNKKLST
                     LTEQDLQNIVNKAYAAGLSKKTLSCICSDLRALCKFCRAAKLSTFNPEGLRVPAGARL
                     KGKKVLQPSDLITLFNVDTTLYKGQTVPDDYINAYRFQVLTGLRPGELVGLQWGDIQG
                     NTVHVQRAVNVRGEVTKGKNQNAVRSFVLSRMARRVLDAQRRITGHQESVFCILTEYN
                     YYWRWKVYCKANGLTVVTPYELRHTFVSVVKTLPAGEVKGLVGHSLDMDTFGVYGHAL
                     TGEAEATAEAVEGTFLKILNNA"
     gene            complement(7679..7759)
                     /locus_tag="ACZPAX_02460"
     tRNA            complement(7679..7759)
                     /locus_tag="ACZPAX_02460"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7723..7725),aa:Leu,seq:tag)
     gene            8215..9102
                     /locus_tag="ACZPAX_02465"
     CDS             8215..9102
                     /locus_tag="ACZPAX_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009983138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HIR1112792.1"
                     /translation="MKQNLLAKFLPFNFLMLLVAGIINAIGVTLFIAPVQLYDSGISG
                     TSILLSQLTPDYLSLSFFLVLLNVPLLLFGFKRQGAVFSVYAIFAVCVYSAAAWLITD
                     VLPIDVSIASPLAGTDLFLCALFGGIISGVGSGLAIRYGGAMDGIEVVAVIFAKPLGL
                     SVGTFVLIYNVLLYIICGVVMGSWILPLYSIVTYAAGSKTVDFIVDGLDSAKAAMIIT
                     TNPDEIARTVSEEFGTGLTILDAHGFYSDTPKTVLYVVVNRFQTVKLKTFVKAIDPNA
                     YMAISPVADLLHGSKEESV"
     gene            9199..10413
                     /gene="spoIVB"
                     /locus_tag="ACZPAX_02470"
     CDS             9199..10413
                     /gene="spoIVB"
                     /locus_tag="ACZPAX_02470"
                     /EC_number="3.4.21.116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIVB peptidase"
                     /protein_id="HIR1112793.1"
                     /translation="MCKHKFLRRALAVLAACLLLGAACLAGLAKALPDTFYIDGALTD
                     LKIAAMPFLSVAQPRQGSMAADNAVADKSGNVTLTLLGVVPIKTVRAVSTPRRTVQVC
                     GTPFGVKMFSDGALIVAFSDRYTALGSENPAKAAGLRLGDWIVSAGGRPVRSNDDLTA
                     AIQAADGAPLTVVYRRDGVQHTAALTPAQDEKGRYKAGVWVRDSGAGIGTMSFVDAQH
                     GTFAGLGHSISDTDTGTELTLLSGEIVPVTITGCIRGAAGSPGELRGEFSAKAVGQVL
                     ANDAAGVYGSYTGGTIGQAVPVANVQEVTPGEAELWTTVQGTTAQPYKVRIERVTMTG
                     SDPNRNLLIKVTDERLLEKTGGIVQGMSGSPIVQNGQLAAVLTHVLVNDPAKGYAIFA
                     ATMLEKADAVIK"
     gene            10692..11255
                     /locus_tag="ACZPAX_02475"
     CDS             10692..11255
                     /locus_tag="ACZPAX_02475"
                     /inference="COORDINATES: protein motif:HMM:NF012301.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0042173 - regulation of sporulation
                     resulting in formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation initiation factor Spo0A C-terminal
                     domain-containing protein"
                     /protein_id="HIR1112794.1"
                     /translation="MEKIKFVMTDTDTQVSDACRRALEGKGVSVTVCEKNGTKALDAL
                     LAIHPQAVLLDAFMPDLDAITVKQRYEAQNAGGSRTTFFVTGAFQSEEIEQELLDSGF
                     SFFFVKPFDENVLVSRVLKAAGNTIRPQIVSQDSDELTVTEILHQIGVPAHIKGYQFL
                     RDAILLTISDHVYINAVTQRLDRILFV"
BASE COUNT         2699 a         3135 c         3240 g         2195 t
ORIGIN      
        1 cgtttacgac gcttttacga cgcgaaaaat aacatgcttg aacatattga gacattataa
       61 aacgtagaaa aaatagcgta tctacgttta tggttcgcag atacgctctc tttgaaacga
      121 tgcggctaac aggacttgaa cgatttaacg tcaaattttc tgctaaatcc gcatataaaa
      181 acacagaatc cgcgcatatt ttccgcaaat tctaaccgga aaagaagcgc ggattttttc
      241 tttaaaattt gcaagtgcgg tcaaaagtgc ggtcaattac cgcaccccgc acggctgcac
      301 caaatgcgag cttttattta gtatggcaac acttgccggg cgggtcaaga gctgcgaaaa
      361 acgctaacca cacccactgc ccttgttttt cactaaacac aattttataa taaaagcatc
      421 gactagccga cgaaaaaacc tgtcacattt ttatatgcgg atgggtttat aatttaatca
      481 gaaaagcgga aaaatgaaaa acctgtccca ctttcacaat ttcagcgcgt tataattatc
      541 ttgtaaaaag gatttaaaca ccgaggaggc taaaaatgac aaatttcgta ttacttcgcc
      601 aacagatgat tgcccacgac ttccaaatga aggatattgc ggaggcggca ggccttagcc
      661 ggagcgcatt ttccgctaga atccaccgtc gagcggattt cacgaccggg gagatgctgc
      721 gcatcggcaa ggtcctgggc ctccagccgg aggactatta caatgtgttt atggccgagg
      781 ccgacaccca gacgggggcg cgagcatgag tacaggccca caagttgcta tcaactacga
      841 caatatcccg cccgaaatgc aggcccgcaa acaatgggtg ctgtggggtg ttgacgctga
      901 caatcaaaag gccccgtgcc aaatcgtcaa ccctgcacgc ggcgcgaaag cgaataaccc
      961 cctgacgtgg ggcacgtttg aggccgcgcg gtgctctgtg gaactgctgg gcgaggcgca
     1021 gggcatcgga tacgagatca ccccggcgga cggacttgtg gcaatcgact ttgaccactg
     1081 cattgaccca gacggccaac tgatggagcc gttcgcaacg tggaaagaag cccttcccgg
     1141 atattgggaa ttatcgcaga gcggccacgg cctgcacggt tttgtacgtg gcacgctgcc
     1201 cgccgggtgt ggcaataagc gccagataga cccacaggat aaggccgacg aacgggaagt
     1261 tgaactgtac atcaaagagc ggtatattgc cctgaccggg gcactgtggg gcgcacagac
     1321 gccgcttgca gaggctcccg acgcacaggc gggcgttgac gtgattcttg gacagatgca
     1381 catgcgcccc gaggacgtcc aggcggaggc cgcgctgccc gatgtaccag accgcccagc
     1441 ggcagaaatc accgcgatta tagcagcggc actcgcaaaa agccctgact tctacacatt
     1501 gtggaatggg gcagaacacc cgggcaagca tggcgacgaa agcaaggatg acatggcttt
     1561 tgtttgctct gcatggaagg ccacagaata tcagctttcc gccggggact tgatgcacgg
     1621 actggacgct tcaccgtggg cgcagaccaa aaccaacgac gctgaaaaag accattggga
     1681 taaatggttt gcttgtggag cgcgtaagga tggcgcgtac cgtagaaata ccgctttagc
     1741 ggcataccag cgggcaaagc aggaagaggc ggaacagctt gacgggtttg ctgcgctggc
     1801 cgcgaatctc ccggcgggcg gggtcttcac cgctgccgaa ctggacaaaa tggacttgcc
     1861 gccggtgcag tgggccgtgc ccggcctgct gcccgcgggc ctgtcgtttc tagttgctgc
     1921 gcccaagatg ggcaagtcgt ggcttgcttt ggatttgtgc ctgtctgtgg cctctggcgg
     1981 tgagtggctg ggccgcaagg tgaaccaggg gcccacactt tacctcgatt tggaggacag
     2041 cgccaaccgc gcacaggcgc gtatgcgcac gttgctggac ggctttaccg ccgcgcctga
     2101 aacttgcact ttccgactac tggccccgat actgggccca gagcttttta aaattctgga
     2161 tgagtgggta gccgccaacc ccggcgcaaa agtcgtttgc attgatactt tccagaaaat
     2221 taaaccttct gccgcgaaaa atgagacgtc ttatagcgca gactatcgca tttgcgcccc
     2281 gttgcaaagc tgggcacaac agcacaacat ttgcgtgctg ttggttcacc acaccaacaa
     2341 gaacaaaggc tccacagata ttttcgagag cgtcaacggt tcacagggcc tcacaggtac
     2401 ggcggacgct gtgctgctgc tgacaaaaag cgagggccga tttgcagagg atgctacgct
     2461 gtccgtaact ggccgtgacg tggacatgga acgatacctc gcacggtttg acaaagatac
     2521 ctgccattgg gaactgctgg gcacggtaga cgaacaggcc cgccagagct ttgaaaactc
     2581 cccggcggtg ctgactgtta aggagttgac cgtcgggggc aagtggcagg gtacagtgca
     2641 ggaactggcc gacgagatac tggaacgata cccggacacc gaattaccag agagcgcgca
     2701 ggggctgcgc agttactttg ctaaggtctg gcctgcgcta aaatgtgcgg gcatcaagca
     2761 cacaacccac aggggggcaa agcagcgaac ccacacatta agccgtgcca gatagctata
     2821 taaggcgtat cgtcatatcg tcatatcgtc acggcaatct acaaaaagcg ccctcaaagg
     2881 cccctgcttg tgacgatgca atgacgatgc aatgacgata ccacaacccc cacaaagatt
     2941 atacctatag tatttgtgac gatatgacga ttgtgacgat acccataggg tatacccatt
     3001 tgtgggcttt tttgtggatg acgatgcaat gacgattata gagcactttt aggaggttac
     3061 gcatatatgc caaatcaaat ttaccgttta tttctacctg tggccacgtg gcgcacacgc
     3121 cgcggcgagg tcgaattgca gcgggaactc tgctgcctgc cacgtagcga gaacgacctg
     3181 cgccgcctca tcaacaccgg ccaggccgcc ccggcggtgc gtgggagcta ctgcgtgcag
     3241 tttggcggag ggggccacta cttcaacgac caccgggccg ctctggccta cctctgcacc
     3301 cgatggggcg gggagttcat catgcgccac ctggacgacg ctgtggagcg agctgcccac
     3361 cgccgcgtta ttgcaatctt tgaggccagg ctcgaggccg aaaagtggca gaatcgggca
     3421 aaccccggcg gaggtgatac tcagtgttga ccccaaacca acgccgcgcc ctggcggctc
     3481 tgctgacctg caagagccgc gccgaggcct cccggcaatg cgggctgggc ctgcgcacga
     3541 tacaagacta cgagaaacag ccggagttcg ccgacgccct agagcgcggc aggcgggagc
     3601 tgctggccga tgctgcccac aggatggccg ccgggtactc tgacaccgtg gacaccctgc
     3661 aagagatcgt agagaacccc gacacgccgg acgcctcccg cgtcgctgct gctcgtgccc
     3721 tgttggatta tgggctaaaa tttgccgaaa taacggatat taacaagcgc ttagacaaat
     3781 tggagggctt aatgtgaaag acgctcttgc acgcctggag gccctggagc ggggccaacc
     3841 tggcgatctg ctgctgatcg tcacagaacg gcagacgccg gaggggccgc gctatgagct
     3901 gccaagcggc gatctgctgc ccctgcccga tgtttacgca tacatgaggc tgcacggtca
     3961 aggcgtcctg ctgattgacg atttgccgga gttgccgaaa ccgaaacgag ggaggaagcc
     4021 gaaacatgag cagtaagtat ttgctggaat ccctgcaccg ccagaccgcc acaggcttga
     4081 ccgtgggtgt ggaggacagc tttttacaca acaccgtccg tctagtatac cgcgcccggc
     4141 ctgatggcgt tattacatcc tggcaagtcg aggggccaga cctgcccgcc cctgctgtat
     4201 tcaagaccga ggcccaggcc cgcgcgttca tccgtggccg ctggggcatc tatccccaca
     4261 caaagaaatg gggcgagcca tgagccgccc agtcccgccg gggaaggtca agaggcctgc
     4321 acccatacga accaaaccac aggcccgcaa gggccagaag cggccccaca gcggggccag
     4381 acaatccaca agagtttagg aggatttacc atgaactatg tacaagaagc ccgcaaaatc
     4441 atcaccgact acttcaccgc catgaagccg tccgagcagc tgctgaaaga agccgccgac
     4501 gaactgcgcc agggccacat cacagaggca tacgccaaag agttggccaa ccacgccacg
     4561 aacgagaagc gcaccctgcg tgataatgcg tggtcacagc tggaggccct gctgcgcaag
     4621 tttgcccttg ccgccgggct tgcagatgca ccaaacggcg acgccctcca aggcggcgac
     4681 tacaagctgt tggcggacaa ctttcccatg agcgcggaag agttcgccgc cctgtgtgag
     4741 cgcaacaaga acaaccccac gatgctgcgc aaggctatgg agtacggcaa caaaaacggc
     4801 ggcctggccc cctacgccaa aaaatattac cgctctgccc atgaccgggt gcagctgttc
     4861 aaaacgttgg tgcagcgttg cggcgcggtg ctggatgcag agccgaccag ccccacccgc
     4921 ggggacgcct attggaacat gatcgcccgc gacgtcgagc cgtgggcgac gctgcgagcg
     4981 ccaggaaggg ccaacgcatg gaactggaca aactacttgc acaggtggag gacgcccgca
     5041 aggcccctct gcgcgtgtgc tgccgaaccc cggcgggtga gttgatcgtc accagcgtgg
     5101 aggactgcgc ccgccagggc tgccgctatt tccacatcgt ggcggacgac ctggacaccc
     5161 tgctggacag agaactgaac cacagaccat aagcaccaca gagatgcacc gcgatggcgg
     5221 cgactagctg cgcattggat gacgtgcaac gcatacccaa cgaccgcaaa gaaacgcccc
     5281 acagcaccgc aaacgggtca ctgtggggcg ttctctgtat tacttcagca tttcctctgc
     5341 ctctggcggc agagtacccg cgccctctgt gggctgctct cggtccatgc gctgggccac
     5401 cgcgcccaca atgtaggcgt tgaggctttc cccggcggca tctgctgccg cctggactgc
     5461 tgccttgttc tgtggtgcat ccttgcgcag aatcaaattc acgcggtcat aggctttcgc
     5521 aatgtatttg tttttgctgg ctgcgcttgt ttttcctccc atgctgtcac ctccaaacgt
     5581 gggcatagtt atacaagatt atgataccgc gatttttgta tatgcgcaag tatgcagtat
     5641 gtacaatata cgagcatcta tgcgcaagta tatttgtgcg atttgtgcat tgcatctatg
     5701 cgtatgtagg catataatta tagtcacagg aacaccaaca tacagagcac aggaggcaca
     5761 gaaccatgac aagctatgaa gttacgtatg aaatgcaaaa tggtgagcac gacacaataa
     5821 acgttgcatc caccaaccta atcaaggccg cgcagttcgc cgagaacttc ctttccgtcc
     5881 gcgtaaaagg ttatagctgc cttgtggaag tcaaagaaca catttacgac taagccgaaa
     5941 cgcccgcaag ggcatccgcc ggggatagcc tcccggcgct gatgatggca ggccacaggg
     6001 tgctgcaaca cccagagcgc ggcaacgctc aaaccccaca ggcatttgat agaatgaccc
     6061 gacccagcac aaaagccgaa accctccccg cctgtggagg gtacaccggg ggcgtcctcc
     6121 ccggtgctga tgatggcagg acacagaacg gaggcaccac agatgctaga caaatttgaa
     6181 ccgtggacga cgcaggcgga ggcgctgcgc acccgccacg ttatccaggt aagccctaag
     6241 actcatgact ttctcgacca actgatggac gtctatcagg cgcgacccct gaaccccgct
     6301 tcccgtgtgg cgctgctaga cctgcttacc gtcgtttttc tggcgggtga aatcagcggg
     6361 aagcgcaaag accgcgcccg ccgcaaagga ggcgaacgcc aatgaacatc acagacttga
     6421 ccgagcgcga gaaagctatt cttgcagaat ttcgcagtat gaccgaggaa cagcaagaca
     6481 cctgcctgcg tgacttgcaa gaactactaa agaagaagga ggcagaacca caatgaccat
     6541 gagaaccaac agcgccaagt ggacgggcac acgctggcgc atcgacgttc agaaggacgg
     6601 aacccgcaag agcttttaca gttccaagcc tggccgcaca ggccagaggg aggccaacgc
     6661 caaggccgac aagtggctga caactggcgt atcagacacc gccctgcgcg tctcccaggc
     6721 ctacgctatg tggtatgaga cacaacagga gacaaccagc gagggcaact gccgcaatat
     6781 cgccacacgc tggcgacact ggattgagcc gaacattggc aataagaagc tatccaccct
     6841 gaccgaacag gacttgcaga acatcgttaa taaggcctac gcggcaggcc tgtctaaaaa
     6901 gacactatcc tgcatctgtt cagacctgcg tgcgctgtgc aagttctgcc gcgctgccaa
     6961 actctccacc ttcaaccccg agggcttgcg cgtgcccgcc ggggcacgtc tcaaaggcaa
     7021 gaaggttttg cagccctctg acctcataac cctgttcaat gtggacacca cactgtacaa
     7081 aggccagacc gtcccagatg attatataaa cgcctaccgt ttccaggtgc ttacaggcct
     7141 gcgtcccggt gagcttgtgg gcctgcaatg gggagatatc caagggaaca ccgtccatgt
     7201 acagcgggca gtcaacgtgc gaggggaagt caccaaggga aagaaccaga acgccgtccg
     7261 atcgttcgta ctgtctcgta tggcccggcg agtgctggat gcacagcgcc ggatcaccgg
     7321 acaccaagaa agcgtgtttt gcatcctgac agagtataac tattactggc gctggaaagt
     7381 ctactgcaag gccaacggct tgaccgttgt gacgccctac gagttacgcc acacgtttgt
     7441 ctcagtggtc aaaacgttac ccgccgggga ggtcaaaggc cttgtgggcc acagcctgga
     7501 catggatacc tttggagtgt atgggcacgc tctgactggc gaggcagagg ccacagcgga
     7561 ggcagtagag ggaacatttt taaagattct gaataacgcc taaagaagtg cggtcaaaaa
     7621 tgcggtcaag aaaacaaaaa agacgctgaa acgaacgttt caacgccgat tttgcaagtg
     7681 cggctaacag gacttgaacc tgcacgtcgt ggacactgga ccctaaaacc agcgcgtctg
     7741 ccaattccgc catagccgca taataaaaat agtttaccaa gtttcggtga atttgtcaat
     7801 gcaaaatgtt gattgacacc gcgccccaag acgattgttc tataagaaaa gggcatcacc
     7861 tatgcaatac acaaaactgg gatacaccga tattgaaatt tccaaggtct gcgtgggttg
     7921 catgagcttt ggcggggcgg gcaaagtgcg ggcgctggat gtggcatgag cgcggaggat
     7981 atagccgccc tggcggaatg ctgcctgccg catcccattg tgggcaccac caacgctaac
     8041 ctgccgcagg gagttgtgct gctggacgaa aaaaagtaaa atagaaatgg gattgcacac
     8101 gaaaaattgt acctactgtg tgcgcttttt acattctgtc tatggacgcg caacccggtt
     8161 atacggtata ctatacccaa accgcccgct cacctgtgac tacggaggta caccttgaaa
     8221 caaaacctgc ttgccaaatt cttgcccttc aattttttga tgctgctcgt cgcgggcatc
     8281 atcaacgcta ttggtgttac gctgttcatt gcaccggtgc agctgtatga cagcggcatt
     8341 tccggcacat cgatcctgct gtcccagctg acgccggatt atctctcgct gtccttcttt
     8401 ctggtgctgc tcaacgtgcc gctgctgctg ttcggattca agcggcaggg agcggtgttc
     8461 tctgtctacg ccatttttgc ggtctgcgtc tactcggcgg cggcttggct tatcaccgac
     8521 gtgttaccca tcgatgtgag cattgcgtcg ccactggcgg gaacagacct gttcctctgc
     8581 gcgttgttcg gcggcattat ttcgggcgtc ggcagcggcc tggcgatacg ctacggcggc
     8641 gcgatggacg gcattgaggt cgtggcggtc atttttgcca agccgctggg gctttcggtg
     8701 ggcacgtttg tcttgattta taacgtgctg ctgtacatta tatgcggcgt ggtcatgggc
     8761 agctggatac tgccactcta ctctatcgtg acctacgcgg cggggtccaa gacggtggac
     8821 ttcatcgtgg acggcttgga cagtgctaag gcggccatga tcattacgac caacccggat
     8881 gaaatcgccc gcaccgtgtc ggaagagttc ggcaccggcc tgacgattct cgacgcccac
     8941 gggttttact cagacacccc taaaacagta ctctatgtcg tggtcaaccg cttccagact
     9001 gtcaagctga aaacgttcgt taaggccatt gaccccaacg cctacatggc catcagcccg
     9061 gtggctgacc tgctgcacgg cagtaaggaa gaatctgtct gaccccaagc ccctgcgccc
     9121 actgcggtgc aggggctttt tgccgacata atccgcccat gccctgcaca tcctaatgca
     9181 aaagcgaggg aatgtgctat gtgcaaacac aaatttttgc ggcgggcatt ggcagtgttg
     9241 gccgcctgcc tgctgctggg ggcagcctgt ctggctggcc ttgccaaggc actgccggat
     9301 acgttttaca tcgacggtgc actgaccgat ttaaaaatcg ccgccatgcc gtttctgtcc
     9361 gtcgcgcagc cccggcaggg gagcatggcg gcagacaacg ccgtggccga taaaagcggc
     9421 aacgtgacgc tgacattgct gggggttgtg cccatcaaga cggtgcgcgc ggtaagcacc
     9481 ccacgccgca cggtgcaggt ctgtggtacg ccgtttgggg tcaaaatgtt ctccgacggg
     9541 gcactgattg tggcgttctc cgatcgctac acggcattgg gcagtgagaa cccggccaag
     9601 gctgcaggcc tgcggctggg agattggatc gtctcggcag gagggcggcc tgtgcgcagc
     9661 aacgatgacc tgacggcggc tatccaggcg gcggacggtg caccgctgac cgtagtatat
     9721 cgccgtgatg gtgtacagca cacggcggct ctgacaccgg cacaggacga aaagggacgc
     9781 tataaagcgg gcgtctgggt gcgggactct ggggcgggca tcggcacaat gagctttgta
     9841 gacgcccagc acggcacctt tgcaggactg ggccattcca tcagcgatac cgacaccggc
     9901 actgagctga cgctgctttc gggtgaaatt gtcccggtga cgatcaccgg ctgtatccgg
     9961 ggtgcggcgg gcagcccggg ggaactgcgg ggcgagttct ccgctaaggc ggtgggccag
    10021 gtgttggcaa acgatgctgc cggggtgtac ggcagctata ccggcggtac gatcggccag
    10081 gctgtgccgg tggcgaatgt gcaggaagtc acacccggcg aggcggaatt gtggacgact
    10141 gtgcagggaa cgactgcgca gccctataaa gtacggatcg agagggtgac gatgaccggc
    10201 agcgacccga accgcaacct gcttattaaa gtgacggatg agcggttact ggaaaaaacc
    10261 ggaggcattg tgcagggcat gagcggctcg cctattgtgc agaatgggca gctggcagcc
    10321 gtgctgaccc atgtgctggt caatgaccca gcgaagggct acgccatctt tgcggcaacg
    10381 atgctggaaa aagcggatgc tgtcataaag tgatgagcag tgccctatct acccgaacag
    10441 agcgggaaaa gagttgcctg ggaaaatgac gtaaaatgga atagcgtgac gctgcgtgta
    10501 atattgcggc gaattgcggc gaaatatagg aaaacttgtc gaacgaaagt tggccgaaat
    10561 tatgaaaaaa tgctccatac cctattgcag atagtggaaa aacatggtaa aatttccaat
    10621 gtaagcagag cttgagcaaa gtttacgcgc aaaggaacgc gcagctcaga aagtatggag
    10681 gatatgtgac gatggaaaag atcaaatttg tgatgaccga cactgacacg caggtgtccg
    10741 atgcttgcag acgtgcgctg gaaggaaagg gtgtttccgt gaccgtctgt gagaaaaacg
    10801 gcaccaaggc gctggatgca ctgctggcaa tccacccgca ggccgtgctt ctggatgctt
    10861 ttatgcctga cctggatgca atcacggtca agcagcgtta cgaggctcag aacgcgggcg
    10921 gcagccgcac cacgttcttc gtcaccggcg cgtttcagag cgaggagatc gagcaggaac
    10981 tgctggacag cgggttctcg ttcttcttcg tcaagccctt tgatgaaaat gtactggtaa
    11041 gccgcgtcct gaaagcggcc ggcaacacga tccgcccgca gatcgtgtcc caggacagcg
    11101 acgaactcac cgtgacggag atcctgcacc agatcggcgt accggcgcac atcaagggct
    11161 accagttctt gcgcgatgca attttgctga ccatcagcga ccatgtgtac atcaatgccg
    11221 tgacgcagcg cctcgacagg attctgtttg tttgagtgca tttggaaca
//
LOCUS       DBMDSQ010000036        11219 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_1733_length_11219_cov_2.475175, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000036 DBMDSQ010000000
VERSION     DBMDSQ010000036.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 11219)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 11219)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11219
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1733_length_11219_cov_2.475175"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..318)
                     /locus_tag="ACZPAX_02480"
     CDS             complement(<1..318)
                     /locus_tag="ACZPAX_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter MgtE N-terminal
                     domain-containing protein"
                     /protein_id="HIR1112795.1"
                     /translation="MSDTAILTLRTMLANLDDAKKYQSLRDVMSTLPAPDLAAVFEDL
                     PPEKMPVLFRLCPKDLAAEIFAELTPESQQNLIDGLTDKELKAVVDDLFVDDATDLVE
                     EMPA"
     gene            693..1718
                     /locus_tag="ACZPAX_02485"
     CDS             693..1718
                     /locus_tag="ACZPAX_02485"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019161037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S66 family peptidase"
                     /protein_id="HIR1112796.1"
                     /translation="MIRNIAVVSLSRGVLGEAFAQHEVEIGLQRLAGYGVNVRFMPNA
                     RRGIDYLTAHPEARAADLLQAFRDPGIDMILCAIGGDDTYRLLPYLFDHDELRRAVTG
                     KVFLGFSDTTVNHMMLHKVGLPTFYGQAFLPDVCELSPAMMPYTEHYFTELLRTGTIR
                     EVTPSDVWYESRTDFSPSQIGIAPATHPDDGFRLLQGSPVFSGEILGGCIDTFYDFFD
                     GTRYADMPLLCQKYGLFPTAEDWQGKILLLETSEEQMPPEKYHAALTYLKNVGVFRAV
                     SGVLIGKPMDRQYEDEYKKILVEVINDPKLSIVCNLNIGHAAPRCILPFGVQACVDID
                     AQKITFA"
     gene            complement(1867..2505)
                     /locus_tag="ACZPAX_02490"
     CDS             complement(1867..2505)
                     /locus_tag="ACZPAX_02490"
                     /EC_number="5.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015564555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HIR1112797.1"
                     /translation="MKIALINENSQAAKNALIEATLRKVVEPMGHEVVNYGMYAADDA
                     AQLTYVQNGILAAVLLNSGAADYVITGCGTGEGAMLALNSFPGVICGHVEDPVDAYTF
                     AHVNDGNAVALPFAKGFGWGGELNLEYIFEKLFGFGHGQGYPKERVVPEQRNKKILDG
                     VRAVAFRDLSDILKDLDRDLVKGAFAGEHFAEYFFPACKDEKLAETVKELMA"
     gene            complement(2529..3110)
                     /locus_tag="ACZPAX_02495"
     CDS             complement(2529..3110)
                     /locus_tag="ACZPAX_02495"
                     /inference="COORDINATES: protein motif:HMM:NF012452.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ntn hydrolase family protein"
                     /protein_id="HIR1112798.1"
                     /translation="MSAILAVCSDAFCAMVSDGRFVEEPITGDKPHVIKNDLPKVRKI
                     NRNVMVGFAGDTLAAVQIINAVDEYQPQYLTLEKIVKIMREKAATVQVQPVGVRLIVG
                     GRNRKGQFVMTMMGSADGYEPQTQESRPGAYLIEGACSHTDVGPWLEEEVKPQAASWR
                     SLDDVARTLDGCIAKMAKNDKSINTTYFRQLIR"
     gene            complement(3652..5898)
                     /locus_tag="ACZPAX_02500"
     CDS             complement(3652..5898)
                     /locus_tag="ACZPAX_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007045566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HIR1112799.1"
                     /translation="MKLHDTGVYLVHGVPQTAAPAGVTADEARKGTIAYGILKAHNTS
                     DSMQDLRMKFDSMTSHDITYVGIIQTARASGMKEFPLPYVMTNCHNSLCAVGGTINED
                     DHQFALSAAHKYGGIYVPPNMAVIHSYNREMMSGCGRMILGSDSHTRYGALGTMAVGE
                     GGGELAKQLVGRTYDMAYPGVVAIYLTGKPNPGVGPHDVALALVAATYANGYVKNRVM
                     EFVGPGVANLSADYRNGIDVMTTETTCWSSIWQTDDVTKQYFEQHNRPEAYKELKPAD
                     VAYYDGCIELDLSTVECMIALPMHPSYAYPIRELKANAGKILQAAQDQANKQLGGKVE
                     MDLVGKICADGRIYVDQGVVAGCSGGTYENICAVADIIKGKSCGNGEFKFSVYPDSMP
                     TYLELVKNGAVADIVSAGGIFRECFCGPCFGAGDTPANGEFSIRHTTRNFPNREGSKP
                     GEGQISAVALMDARSIAATAANGGYLTAASELTDVEYTVPEYHFEQGVYDKRVYNGWG
                     HPEPDYELKFGPNIKDWPQQPALTDDLLVKIVSYITDPVTTTDELIPSGETSSFRSNP
                     LRLAEFTLSRKDPAYVPNAKAVKALDEERRAGALPAEIARVYDDLKKRGYTPDAANTN
                     IGSAIFANKPGDGSAREQAASCQRVLGGAANFACEYATKRYRSNCINWGMLPFLLDTP
                     NVLANGDYVYLPGVRKALLNADDELTAIAVKPDGGVVRFTVSLGAMTDAERQILADGC
                     LINYYKNH"
     gene            complement(6140..7132)
                     /locus_tag="ACZPAX_02505"
     CDS             complement(6140..7132)
                     /locus_tag="ACZPAX_02505"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HIR1112800.1"
                     /translation="MKAVLIPGDGIGREIAESVRAVSAALDTGIEWQEFAAGAEYAAE
                     SGELIAPGTLDAIEACGWALKGPTATPIGKGFRSINVQLRQRFSTYANLRPVHTLPGV
                     PTRFDNVDLVIVRENTEDLYKGIEYMVNDEMANGVKLITRPACEKICRFAFDYARKNG
                     RKKVTAVHKANIMKLTDGLFLRTFREVAAEYPDIAADDCIIDALCMKLVQKPEQFDVL
                     VAPNLYGDIISDLCAGLVGGLGFAPSANIGDTTRIYEAVHGSAPDIAGQDKANPSAIL
                     MAFAMMLNDLGETEKAARLNDAILAQVAEGKVVTADIGGTAGTKEFTQAVISRL"
     gene            7416..8789
                     /locus_tag="ACZPAX_02510"
     CDS             7416..8789
                     /locus_tag="ACZPAX_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007045565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HIR1112801.1"
                     /translation="MYYPDVPALEPDELNLLCNEYMEHNATLDPHLADQMGVKRGLRN
                     LDGTGVLAGITNVSNVIGYDKKPDGTITPIPGRLVYRGIDIDTLAAEADAHDRFMFEE
                     VIWLLLFGSLPTQEQYAKFRKLLEHHRELPQGFADDMILNSPSPNLMNKMARSVLAMY
                     SYDEHAEDNSLPNILRQSINLIAELPTMMVNAYQIKRRVYDRSSMYFHLPTEGQSTAE
                     HILSTYRADQKFTHEEARLLDLCLLVHADHGGGNCSTFTCRVLSSSGTDTYAAISSAI
                     GALKGPKHGGANLKVMHQLDYILENVEDPTNDDEVREFLRKIIRKERGDGSGLIYGMG
                     HAVYTLSDPRAQILKTHAKSLAYKNGYDEEYEMLCSIERLAPQVFAEEKHGPKKVCAN
                     VDLFSGLIYRMLGISEDLFTPLFAIARVPGWCAHRVEEVEFANRIIRPAYQYVGHPQA
                     YVALEER"
     gene            8902..9645
                     /locus_tag="ACZPAX_02515"
     CDS             8902..9645
                     /locus_tag="ACZPAX_02515"
                     /inference="COORDINATES: protein motif:HMM:NF020893.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HIR1112802.1"
                     /translation="MPERANKREKRLAMLVVAAVVVVTVLLIVETFRLLLPGLWSVFS
                     AGDEQALSDYLTSQGRLRSFLTLWFLAVVQVLSLVIPAMPVQLAAGLAYGPWLGFLTS
                     FSASAVANMSVYLLARRLAPVIRRLVADTPKAAKLLDSVRSSRDPWFYTILAFITPGL
                     PNGIIPYAAANAGMRPKQFLAAIFISLPFPTLLTCAAGSFILEGNWMLTVLVGVVLFS
                     FVGILFFNRTRLIARAHQLNDNRKKKAGA"
     gene            complement(9707..10039)
                     /locus_tag="ACZPAX_02520"
     CDS             complement(9707..10039)
                     /locus_tag="ACZPAX_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009065754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter permease"
                     /protein_id="HIR1112803.1"
                     /translation="MPFDVHGALLVAVVAGVTALIRFLPFVVFGGGRPTPRFIVYLAG
                     VLPCAIMAMLVVYCLRGVDLLGPTHGLPELIAAAAVVGLHLWKKNTLLSIAAGTALYM
                     VLVQVVFV"
     gene            complement(10029..10724)
                     /locus_tag="ACZPAX_02525"
     CDS             complement(10029..10724)
                     /locus_tag="ACZPAX_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009065668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HIR1112804.1"
                     /translation="MKTFKYAFVRSVPIMAGYIVLGLGFGVLLQSKGYGAGWALAMGG
                     LIYAGSMQYVTIDLLAGGASLISAALMTLMVNARHLFYGISMLERYKNTGAAKLYLIF
                     ALTDETYSVVCSGDAPEGVDRSRYFFWVSLLDQFYWVVGCVAGALLGSVLPFDTTGID
                     FSMTALFLVVMTEQWKASRDHTPALVGLGVSLVCLLIFGSSNFLIPSMLGITAALTLL
                     RKVTEKKEADHAV"
     gene            complement(10946..>11219)
                     /gene="dnaK"
                     /locus_tag="ACZPAX_02530"
     CDS             complement(10946..>11219)
                     /gene="dnaK"
                     /locus_tag="ACZPAX_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007045562.1"
                     /note="heat shock protein 70; assists in folding of
                     nascent polypeptide chains; refolding of misfolded
                     proteins; utilizes ATPase activity to help fold;
                     co-chaperones are DnaJ and GrpE; multiple copies in some
                     bacteria; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HIR1112805.1"
                     /translation="AKERKAALEALNAGEAALYRVNTALGSKVGKALDRETRGKIKDA
                     ERALERVLRHKKADKLTPADVTAINAAREALSTVAAPLVAQWESEK"
BASE COUNT         2229 a         3366 c         3293 g         2331 t
ORIGIN      
        1 ggcaggcatc tcctcgacaa gatccgtcgc atcgtcaacg aacaggtcat ccacaacggc
       61 cttcaactct ttatcggtca gaccgtcgat gaggttctgc tggctttcgg gcgtcagctc
      121 ggcaaaaatt tcagctgcca ggtccttggg acacaggcgg aacagcaccg gcatcttctc
      181 cggcggcagg tcctcgaaca cggcggccag gtcaggggcg ggcagggtgg acataacgtc
      241 ccgcaggctc tggtactttt tggcatcgtc caggttggcc agcatcgtgc ggagggtcag
      301 gatcgcggta tcagacataa aaaagctgcc tccttttcag gcggcagcca agcacatttt
      361 ggttttgggc aacgcaaaac agagcaacac cgtgggttat ggctaacaca cagtttgctt
      421 tgatgcgccc ctaaaaatat ggtggctgcc gttatttaag atacatcggt acacaggact
      481 gggactaggg gtatccattg tgacaaccac ctcacatata tttgaatttt taccatgagg
      541 cgctattcgc acctatattt actttaccat ccaaagccga attagtcaac ggtaaatttc
      601 ggcatttgac gaaatttttt tccgtgcgca cgactgtcat ctgtgatata ctgggggtgc
      661 attcctacga ccgcttacag ggaggtatcg ccatgattag aaacatcgct gtcgtcagcc
      721 tgtcccgcgg cgtgctggga gaggcgtttg cacagcacga agtcgagatt gggctgcaac
      781 gcctggccgg gtacggcgta aatgtccgct ttatgcccaa cgcccggcgc ggcatcgact
      841 acctgaccgc gcacccggag gcgcgcgccg ccgaccttct gcaggcgttc cgcgacccgg
      901 ggattgacat gatcctctgc gccatcggcg gggacgacac ctatcggctg ctcccctatc
      961 tgttcgacca cgacgaactg cgcagggccg tcaccggcaa agtgtttctc ggcttttccg
     1021 acacaacggt caaccacatg atgctgcaca aggtcgggct gccgacattt tatgggcagg
     1081 catttctgcc ggacgtctgc gaattgagcc ccgcgatgat gccctacacc gagcactatt
     1141 ttacggaatt actgcgcact ggcacgatcc gcgaggtcac gcccagcgat gtctggtacg
     1201 aaagccgcac cgacttcagc ccctcgcaga ttggcattgc tcccgccaca caccccgacg
     1261 acggtttccg gcttttgcag ggcagcccgg tattttctgg ggaaattctc ggcggttgca
     1321 tcgacacttt ctatgatttc ttcgatggta cgcggtatgc agatatgccg ctactgtgcc
     1381 aaaaatacgg tctttttccc acggccgagg actggcaggg gaaaattttg ctgctggaaa
     1441 ccagcgagga gcagatgccg cccgaaaaat accatgcagc attgacatac ctcaaaaacg
     1501 tcggggtgtt ccgtgccgtc agcggcgtgc tgattggcaa accgatggac cgccaatacg
     1561 aggacgagta caagaagatt ttagtcgaag tcatcaacga ccccaagctg tccatcgtct
     1621 gcaacctcaa catcgggcac gccgcgccac ggtgcatttt gccctttggc gtccaagcct
     1681 gtgtagacat tgatgcgcag aagatcactt ttgcttaaag cagcgcacta ctttgggggc
     1741 cgcatctgac ggggcgtcga ggacgccgcc cccctacaaa tacgcggctg tacaaataaa
     1801 aagccgacgc ctggcaggtt gtaccgggcg tcggcaattt ttattttagg taaatcaggc
     1861 cagggcttac gccatcagtt ccttgacagt ctcggccagc ttttcgtcct tgcaggcggg
     1921 gaaaaaatac tcggcgaagt gctcaccggc gaaagcgccc ttgacgaggt cgcggtccag
     1981 atccttcagg atgtcggaca ggtcgcggaa ggcaacagcg cgcacgccgt ccagaatctt
     2041 cttgttgcgc tgctcgggca caacgcgctc cttggggtag ccctggccgt ggccgaagcc
     2101 gaacagcttc tcgaagatgt actcgaggtt cagctcgccg ccccagccaa agcccttggc
     2161 gaagggcagg gccaccgcgt tgccgtcgtt gacgtgggcg aaggtgtagg catcgaccgg
     2221 atcctcgaca tggccgcaga tgacaccggg gaagctgttc agtgccagca tagcgccttc
     2281 gccggtgccg cagccggtga tgacatagtc ggccgcgccg gagttcagca gcacagcggc
     2341 caagatgccg ttctgcacat aggtcagctg ggcggcatca tcggcggcat acatgccgta
     2401 gttgacgacc tcgtggccca tcggctccac gaccttgcgc agggttgctt caatcagggc
     2461 gttcttggcg gcctgactgt tttcgttgat cagtgcgatt ttcatatttt ttctccttta
     2521 cgtgcgtttt atcggatcag ctgacggaaa tacgttgtgt tgatgctctt atcgttcttg
     2581 gccatcttgg caatgcagcc gtccagcgtg cgggctacat cgtccaggct gcgccagctg
     2641 gcggcctggg gcttgacttc ttcttccagc caggggccga cgtcggtgtg ggaacacgcc
     2701 ccctcgatga ggtacgcgcc ggggcggctc tcctgggtct gcggctcgta gccgtcggcg
     2761 ctgcccatca tcgtcatgac gaactggccc ttgcggttgc gcccgcccac aataaggcgc
     2821 acgcccaccg gctgcacctg cacggtggcg gccttttcgc gcatgatctt gacgattttt
     2881 tccagggtca ggtactgggg ctggtactcg tccacggcgt tgatgatctg cacggcggcc
     2941 aacgtatcgc ccgcaaaacc aaccatgacg ttgcggttga tcttgcgcac cttgggcagg
     3001 tcgttcttga taacgtgggg cttgtcgccg gtgatgggtt cctcaacaaa acggccatcg
     3061 ctgaccatgg cgcagaatgc gtcactgcaa acagctaaaa tagcactcat aattttttcg
     3121 ttcctttgct tttaataggg tacttgtatt gtagcacgaa atttgcgcgg gtgcaatggg
     3181 tgcttgactt aacctgcggt gtgcggtata ctaataacag aaaaggcgct gcgacaagcg
     3241 gttggcccga tcaaaatggg atttaggaaa acctaagaac cgtcactttg ccgagtggcg
     3301 gtttttgctt tttacgacta aacttaatgt gatggtcttg cggccaacat agaacgtaaa
     3361 tgtaaatcgc atggtctcac ctcctttcgg gggtggggcc aaccgcctgc cgttgtgtgc
     3421 agcgccttgc cgtctgttcg tacaggcggt ttttgtgtta taccagagtt ttcgacgata
     3481 tacaagcaaa gctgcccgca ggagaacctt cccccgcggg cagcctttta ctttatcgag
     3541 tttatcgagc ctgtaggggc cgcatatgtg cggcccgcgg ctttccgcaa gcactcgtct
     3601 gccttatacg gcagggccgg acatacccgg cccctacagg gcaactctta tttagtgatt
     3661 cttgtaataa ttgatgaggc atccatcggc caggatctgc cgctcggcat cggtcatagc
     3721 gccgaggctg actgtaaagc gaactacgcc gccgtccggc tttacggcga tggcggtcaa
     3781 ctcgtcatcg gcattcagca gcgccttgcg cacgccgggc aggtagacgt aatcgccgtt
     3841 ggccagcacg ttgggcgtat ccagcaggaa gggcagcatg ccccagttga tgcagttgga
     3901 gcggtagcgc ttggtggcgt actcgcaggc aaagttggct gcgccgccca gcacgcgctg
     3961 gcagctggcg gcctgctcgc gggcggaacc gtcaccgggc ttgttggcga agatggccga
     4021 gccgatgttg gtatttgccg catcgggcgt gtagccccgc ttcttcaaat catcgtagac
     4081 acgcgcgatc tcagcgggca gagcgcctgc gcggcgctcc tcatccagcg ccttgacagc
     4141 cttggcattc ggcacatagg ccggatcctt gcgggagagg gtgaactccg ccaggcgcag
     4201 cgggttagag cggaaggacg acgtctcgcc cgagggaatc agctcatcgg tggtggtgac
     4261 cgggtcggtg atgtagctga cgatcttcac gagcaaatca tccgtcaggg cgggctgctg
     4321 gggccagtcc ttgatgttcg ggccaaattt cagctcatag tccggctccg ggtggcccca
     4381 gccgttgtat acgcgcttgt cgtagacacc ctgctcaaag tggtactcgg gcacggtgta
     4441 ctcgacatcg gtcagctcgc tggcggcggt caggtagccg ccgttggccg cggtggcggc
     4501 gatggagcgg gcgtccatca gggcgacggc gctgatctgg ccctcaccgg gcttcgagcc
     4561 ttcgcggttc gggaagttgc gggtcgtgtg gcggatcgag aactcgccgt tggccggggt
     4621 atcgcccgcg ccgaagcagg ggccgcagaa gcactcgcgg aagatgccgc cagcggaaac
     4681 aatgtcggcc accgcgccgt tcttgaccag ttccaggtag gtcggcatac tgtcggggta
     4741 gacgctgaac ttgaactcgc cgttgccgca actcttgcct ttgatgatat ccgccacggc
     4801 acagatattc tcatacgtgc cgccggagca gcccgcgacc acgccctgat ccacatagat
     4861 gcggccatcg gcgcagatct tgccgaccaa gtccatctcg accttgccgc ccagctgctt
     4921 gttggcctgg tcctgagccg cctgcaaaat cttcccggcg ttggctttca gctcgcggat
     4981 ggggtaggcg tagctcgggt gcatcggcag ggcgatcatg cactcaacgg tggacagatc
     5041 cagttcgatg cagccgtcgt agtaggctac atcggcaggt ttcaattctt tgtaggcctc
     5101 cgggcggttg tgctgctcga agtattgttt cgtaacatcg tctgtctgcc agatggagga
     5161 ccagcaggtg gtctcggtgg tcatgacgtc gatgccgttg cggtaatcgg cgctcagatt
     5221 ggcaacgccg gggccgacaa actccatgac cctgttcttc acatagccgt tggcgtaggt
     5281 ggcggccacc agcgccagcg cgacatcatg ggggccgaca ccggggttgg gcttgccggt
     5341 cagatagatg gcgaccacgc cggggtaggc catatcgtag gtgcggccga ccagctgctt
     5401 ggccagctcg ccgccgccct cgccgacggc catcgtgccc agcgcaccgt agcgggtgtg
     5461 ggagtcagag ccgaggatca tccgcccgca gccggacatc atttccctgt tgtagctgtg
     5521 gatgacggcc atattcggcg gaacatagat gccgccgtat ttatgggcag cggagagggc
     5581 aaactggtgg tcgtcctcgt tgatggtgcc gcccacggcg cagaggctgt tgtggcagtt
     5641 cgtcatgacg tagggcagcg gaaattcctt catgccggag gcgcgggccg tctggatgat
     5701 gccgacataa gtaatgtcgt ggctggtcat cgagtcgaat ttcatgcgca ggtcctgcat
     5761 ggaatcgctt gtgttatggg ctttcaggat gccgtaagcg atagtgccct tgcgggcttc
     5821 gtcagccgtg acccctgccg gggcagcggt ctgcggcacg ccatggacaa gataaacgcc
     5881 ggtatcgtgc agtttcatgg gaaaccctcc gttttatatg tatcgccaaa gccttcccct
     5941 tagggggaag gtggccgagc gaagcgaggc cggatgaggg gcagcctgcc cacagccacc
     6001 cggcaaagag acagccacgg caaacgcgcc cctcatcagt ccgctgcgcg gacagcttca
     6061 gtctccgtgc caagtgccgc ctacggcggt tgcactccga cacgcgcctg cgggcgcagc
     6121 ccctcagggg aagcctttat cacagtcggc tgataaccgc ctgcgtaaac tccttcgttc
     6181 ccgccgttcc tccgatatcg gcggtcacga ccttgccctc ggcgacctgt gccagaatag
     6241 catcattcaa acgggcggcc ttttccgtct cgcccaggtc gttcagcatc atggcaaagg
     6301 ccatcaggat ggcgctgggg ttggccttgt cctgcccggc aatgtcgggg gcagagccgt
     6361 ggacagcctc atagatgcgg gtcgtgtcgc caatgttcgc actgggcgca aaacccagac
     6421 cgcccaccag tcctgcgcag aggtcgctga tgatatcgcc gtagaggttc ggcgcgacga
     6481 gcacgtcaaa ctgctcaggc ttctgcacca gcttcatgca gagggcgtcg atgatgcagt
     6541 cgtcggcggc aatgtcgggg tattcggcgg ccacctcgcg gaaggtgcgc aggaacaggc
     6601 cgtcggtcag cttcatgatg ttggccttat gcacggccgt gacctttttg cggccgtttt
     6661 tgcgggcata gtcaaaggca aagcggcaga ttttttcaca cgcagggcgg gtgatgagct
     6721 tcacgccgtt ggccatctcg tcattgacca tatactcgat gcccttgtac agatcctcgg
     6781 tgttctcacg cacaatgacg aggtcgacgt tgtcaaagcg ggtaggtacc cccggcagcg
     6841 tgtgcacagg gcgcaggttt gcgtaggtgg aaaagcgctg acgcagctgg acgttgatgc
     6901 tgcggaagcc cttgccaatg ggggtcgcgg tggggccctt cagcgcccag ccgcaggcct
     6961 caatggcatc cagcgtgccg ggggcgatca gctctccgct ctcggcggcg tactcggcac
     7021 cggcggcaaa ctcctgccac tcgatgccgg tgtccagcgc ggcggagacg gcgcggacgc
     7081 tctctgcaat ttcacggccg atgccgtcgc ccgggatcag aactgctttc ataaaaatac
     7141 tttccccttt ccctgtgttt cttacaatat acataatagt aaccggtttg ggggcggttg
     7201 tcaatgcgca tttttacaca tttcgcggca atctgcccac tagcttaatt atttaaagtg
     7261 ccaacttggc aattttgcaa aatttttaag tttttcttga cgaaagcgcc aatatactat
     7321 aaaatcaatc tatcaggcac ttggtgtaag ggaacgcccg caggggtatg cgcctgtata
     7381 aaaatgcaaa gtgtataaag agaggggtat gtgcgatgta ttacccggac gtcccggctt
     7441 tggagccgga tgaattgaac ctgctctgca atgagtatat ggagcataac gccacgctgg
     7501 atccgcatct ggcggatcag atgggcgtca agcgcggcct gcgcaatctg gacggcaccg
     7561 gcgtactggc cggcatcacg aacgtgtcca acgtcattgg ctatgacaag aagccggacg
     7621 gcaccatcac gcccatcccg ggccggctgg tctaccgcgg catcgacatt gacacgctgg
     7681 ccgccgaagc cgacgcccac gaccgcttta tgtttgagga ggtcatctgg ctgctgctgt
     7741 tcggctctct gcccacgcag gagcagtacg ccaagttccg caagctgctg gagcatcacc
     7801 gcgagctgcc ccagggcttt gccgacgaca tgatcttaaa ctccccttcc cccaacctga
     7861 tgaacaagat ggcgcgaagc gtgttagcca tgtacagcta tgacgagcac gccgaggata
     7921 acagcctgcc caacatcctg cgccagagca tcaacctgat tgccgagctg cccacgatga
     7981 tggtcaacgc ctaccagatc aagcgccgcg tctacgaccg cagcagtatg tacttccacc
     8041 tgcccaccga gggccagagc acagccgaac atattttgag cacctaccgc gccgaccaga
     8101 agttcaccca tgaggaggcg cggctgctgg atctgtgcct gctggtccat gcggatcacg
     8161 gcggcggtaa ctgctcgacc ttcacctgcc gtgtgctgtc cagctccggc accgacacct
     8221 atgcggccat ctcgtcggcc atcggcgcgc tgaaaggccc caagcacggc ggcgcgaacc
     8281 tgaaggtcat gcaccagttg gactacattt tggagaacgt cgaagatccg accaacgacg
     8341 acgaggtccg cgagttcctg cgcaagatca tccgcaagga gcgcggtgac ggcagcggct
     8401 taatttacgg catgggccac gcggtctaca cgttaagcga cccgcgtgcc cagattctca
     8461 agacccacgc caagagcctg gcctacaaga acggctatga cgaggaatac gagatgctgt
     8521 gcagcatcga gcggctggcc ccccaggtct ttgccgagga aaagcacggc ccgaagaagg
     8581 tctgcgccaa cgttgacctg ttcagcgggt tgatttacag aatgctgggc atcagcgagg
     8641 atctgttcac cccgctgttt gccattgccc gtgtgcccgg ctggtgcgcc caccgcgtgg
     8701 aggaagtgga gtttgcgaac cgcatcatcc gccccgccta ccagtacgtc ggccacccgc
     8761 aagcgtatgt ggcgctggaa gagagataag gtatattccc ggtgcagctg caagcagcac
     8821 ttttcccctc agaatttcct tgcatttccc cgctattttt ggtatagtat ttcctgtttt
     8881 cattgcaaca aggagatttt tatgcctgaa cgcgccaaca aaagagaaaa acggctggct
     8941 atgctcgtgg tagcggcggt ggtcgtcgtg acggtgctgc tcatcgtcga gacttttcgc
     9001 ctgctgctgc cggggctgtg gtcggtattt tccgccgggg atgaacaggc cctcagtgat
     9061 tacctgacca gccaggggcg gctgcgcagt tttctgacgc tgtggttcct ggccgtcgtg
     9121 caggtgcttt cgctggtcat tcccgccatg ccggtgcagc tggcggcggg gcttgcctat
     9181 gggccgtggc tcggcttttt gacgagcttt tccgcctccg ccgtcgccaa catgagcgtc
     9241 tacttactgg cccggcggct ggccccggtc atccggcggc tggtggccga cacccccaag
     9301 gctgcgaagc tgctggacag cgtgcgcagc agcagggacc cctggtttta cacgattctg
     9361 gcgttcatca cgccggggct gcccaacggc atcatcccct acgccgccgc caacgccggg
     9421 atgcgcccca agcagttttt ggcggccatc ttcatcagcc tgccctttcc caccctgctg
     9481 acctgcgcgg ccggcagctt tatcctggag ggaaactgga tgctgaccgt gctggtgggc
     9541 gtggtgctgt tcagttttgt gggcattctg ttcttcaacc gcacccggct catcgcccgg
     9601 gcgcaccaac tcaacgataa ccgcaagaaa aaagccgggg catgaaagcc tcggcttttt
     9661 tataaaggtt tcccctaagg gggaaggcat cgccctctac agcgcgttac acaaacacca
     9721 cctgcaccag caccatatac agcgccgtgc ctgcggcgat gctcaacaat gtattcttct
     9781 tccacaaatg cagccctacc accgcagcgg cggcaataag ttccggcagg ccatgggtcg
     9841 ggccgagcag gtccacgccg cgcaggcagt agacaaccag catcgccatg atggcgcagg
     9901 gcagcacacc cgcgaggtag acaataaacc ttggtgtggg ccgcccgccg ccgaacacca
     9961 caaagggcaa aaaccgtatc agcgccgtca cgcccgcgac caccgccaca agcagcgcac
    10021 cgtgtacatc aaacggcatg gtcggcctcc tttttctcgg taactttacg cagcagcgtc
    10081 aaggccgccg taatgcccag catggaaggg atcaggaaat tgctgctgcc gaaaatcaaa
    10141 aggcagacca gcgagacgcc cagccccacc agcgcggggg tgtgatccct gcttgccttc
    10201 cactgctcgg tcatgacgac gaggaacagc gcggtcatgg aaaaatcgat gccggtggta
    10261 tcaaagggaa gcacgctgcc cagcagcgcc cccgccacgc agcccacaac ccagtaaaac
    10321 tgatccagca gcgacaccca gaaaaagtat ctgctccggt caacgccctc gggcgcgtcg
    10381 ccgctgcaca caacggagta agtctcatcc gtcagggcaa aaatcaggta cagcttagcc
    10441 gcgccggtat ttttataacg ttccaacatc gaaataccgt aaaacagatg ccgcgcgttg
    10501 accatcagcg tcatgagcgc cgccgagatc agcgacgcgc cgcccgccaa taaatcgatc
    10561 gtgacatact gcatactgcc cgcatagatc aggccgccca tggccagcgc ccagcccgcg
    10621 ccgtaaccct tgctttgcag cagcacgcca aagcccagcc ccagcacgat gtaccccgcc
    10681 atgataggca cagagcgcac aaaggcgtat ttgaacgttt tcaactttcc atttcccctt
    10741 taacctacta aagtgctatg ttggctagta tagcacaaaa aaattcccta tgcaacaaaa
    10801 aaggcttccc ccgagaggga agctgtccgc gaagcagact gatgcggggc aagcgtgcca
    10861 atgcagccca ttatcgggca gtcatggcaa acccgcccct catccggctg ttcgagccac
    10921 cttcccccaa aacgggaagg caaaatcatt tttcactctc ccactgcgcc accaatggcg
    10981 cggcgacggt ggagagcgcc tcccgggcgg cgttgatggc ggtcacatcc gccggggtga
    11041 gcttatcggc ttttttatgg cgtaatacgc gctccaacgc gcgttctgcg tccttgattt
    11101 ttccgcgcgt ctcgcggtcc agagccttgc cgaccttgct gcccagcgct gtgttcacgc
    11161 ggtagagcgc ggcctcgccc gcgttcaggg cctccagggc ggccttgcgc tccttggcc
//
LOCUS       DBMDSQ010000037        10689 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_1817_length_10689_cov_2.776728, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000037 DBMDSQ010000000
VERSION     DBMDSQ010000037.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 10689)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 10689)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10689
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1817_length_10689_cov_2.776728"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..762
                     /locus_tag="ACZPAX_02535"
     CDS             <1..762
                     /locus_tag="ACZPAX_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009322425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1112806.1"
                     /translation="IEGDYYTLQPYDIVLLRPSLLHKTEYPSGGDCRRLIINFRVPDD
                     SPALHEMLQSCLQPFGAEVPIYRFTGETRVQAFSHLNAIFTLGKQPMTPLTQQFIHCH
                     FLQFLATVAQHAGESSYEPQELSDSITQKVYAVTSYIHRHYASELSLEYLAEKFFISP
                     YYLSHQFRRVTGFSLINYIQITRVRNAQQLLLYTDMKIADITTSCGFTSFSQFNRVFN
                     KFCHTSPSQFRVNGVVTPITAIPFEKEPSPGSTKV"
     gene            complement(803..3319)
                     /locus_tag="ACZPAX_02540"
     CDS             complement(803..3319)
                     /locus_tag="ACZPAX_02540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1112807.1"
                     /translation="MANELHFPLFHGYVDRWLVSGVTETPVITKPTALHGAENEKLNT
                     APKDKPGEEQFKAERRAKATAWPAQLDTRPGGLVGGADNATPFAVYWPFDDIGVTYAW
                     DCETPACLSAWAYTELDAAEAGSVPFDLITCGGAALWLNGEKLTEVTPYHHNEPVPTP
                     LTLPLQKGRNKLLVFFDNYAERDATIILRLRWKGEGTAPEQVIPVGEADAARLAEAEH
                     LMRSLAFHRNHFTAGPVLLNYDGADIAESYHLAFVGGTEENVKGGVLFRREADTLPHA
                     SQISLGDCKTFPLAFLLLQVTTEVEGIRLTRPISVEIHTESVLPRALPTVAERKRQAL
                     ELLARWGEQNTNRALAILATGGDTAEAEKLLAVQSDYIRRRFDCSDFYLVYYPYILRT
                     FGKRGSGILSEKTENELTDCLLGFRYWIDEPGNDAMWFWSENHALMFHTCQLLAGELF
                     PDEVFTNSGLTGRQMQAKAKNMLYDWFVTFRKEGFTEWNSSPYLPIDTLGFGSLYAFA
                     QDPAMRELGREGMDFAYYLLAVHSQQGIFASSSGRTYIKEQFGNWSNCPSGLSWIGYG
                     YGVPGHAGKGITSYCLSDYEPPKEFAQWFSIPRGKEMICQTTQGNDGYVDLYTYKTAD
                     YMMTSANDFHPGEPGHQENPFQLTFNAVAQLWVTHPGERVLFGAARPSYWAGNGTLPK
                     VNQYKAFAGLIYDIAPEHPVDFTHLYLPSMEFDRCEVEGKWAFVQLGSAYAAVYCSNG
                     MRMQDFGSNKDRECIAEGRRCIWLVRAAQADEFAAFEDFKAAMRGAALDADAEGRRYR
                     FVDPVYGELRCQWDETLYVNDAPMQYSGFDNYGKLTMQEK"
     gene            complement(3435..4751)
                     /locus_tag="ACZPAX_02545"
     CDS             complement(3435..4751)
                     /locus_tag="ACZPAX_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009322420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR1112808.1"
                     /translation="MKKALSLALAAAMAASLAACGGSPAASTASSAATTDTAASGAAS
                     AGGQTLKVMLSEEPGEGDAFATTLNKWADETGNTVDIMVIPYDDQLTKFPLMAKNNDL
                     PDLLSTTRLARLYPDEFEDLGKCIDTSIFEPQALQIISQNYLTDKLSVLPQQFTITNV
                     FYNKDAFEAAGIECPTVDDRWTMDEVYEAAKKLQDSGAVKYGMAMDYSRARYDNLMYM
                     NGGSLVEKDGDSFKVVADSQQNIDALQAFVDANNSGIMPKAIWAGGSSDNPGDYFKNG
                     DVGIYFSGSWNYNPFVQDITSFKFGVMPSPVGSAGGSAILGGAGLAVPTNAKNKDLAL
                     EFLKWFYTDKNFADYLALDKGLSSLKSVTYEPADPDQAADYKVLQAEVGQTTDAFAID
                     ESSEWRNYYDNEYRDALKQSVNGDLTPSDALTEFATALADKAGWAH"
     gene            5352..8513
                     /locus_tag="ACZPAX_02550"
     CDS             5352..8513
                     /locus_tag="ACZPAX_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009322416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HIR1112809.1"
                     /translation="MQNTELRRRARPVLDALRTLALQGRTPVLGLRKKSCPYHSWDTV
                     QEDPATAPGWTPFAPGDAIGGVEAHYCFRGGITAPAGSAGRHLVCLVSTGATDIWNNN
                     NPQFLAYLNGALVCGLDVNHTEFDLPSAAAEGQTWQLGLYTYCNTPAQDVFLKVETAV
                     RDDEITGLYYDLKAPYEVLVQLDEGDTNAIGIGRYLEKALDMLDLRDPYSAAFYASVA
                     AARRYMKEEFYESFCQRQPVTVDCVGHTHIDVAWLWTLAQTREKAIRSFATVNYLMDR
                     YPEYKFLSSQPQLYDFVRKDCPALFEKIRARVADGHWEPEGGMWLESDCNMASGESLI
                     RQFLHGQEFFGREFGKQGHILWLPDAFGFSGALPQIMAQCGMDWFMTTKMAWNDTNCM
                     PHDLTRWRGIDGTEVLAYFISTKDYDKRPDRNPKPSFNTTYNGLLNPRQVMGCWQRFQ
                     DKELTQDVLQCYGYGDGGGGVTAEMLEINRRMEKGIPGAPQTRLTHAAPYFDKLKQHL
                     DETQADLPCWHGEMYFEYHRGVFTSQGRNKRANRAAEFANLTAETAAALAESLRTGYA
                     YPAAALHRNWELTLLNQFHDILPGSALGEVYEVSQQQYGEILASDARITDDALHTVAA
                     LVKTDRPGVVVFNQLGFARDTVVRVACGASGITDGGHPLPCHTENGALTFVAKDLPAK
                     GWRFYPFADAEPETPCAEVAENDGGYIIDTPLYRIVFNGCGEITALLDKEADRELIPA
                     GQCANELQLFEDRPDEYDAWNLEKYYRRHRFALDGAARLTVAENSPVCCRLHLERAIS
                     RSQLVQDITLYPHSRRIDFATHIDWHEQHVLLKAAFPVDICADRAQYDIQFGSIARDT
                     HTNTSWDEARFEVCAHKWADLSEPGYGAALLNDGRYGYDVHDGVLRLSLLRAATYPDP
                     HADQGAHDFTYALLPHLGDWRQGGVIPAGYDLNAPALPLAVAAQPAGTLPAAYSQFRV
                     DAPNVVLETVKKAEDGSGLILRLYESWGTRTRAALALPDDAAAVRCTPMEQPLPGEEA
                     VQHSLPLTLHPFEICTIHVQFAQ"
     gene            8756..9634
                     /locus_tag="ACZPAX_02555"
     CDS             8756..9634
                     /locus_tag="ACZPAX_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009322415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR1112810.1"
                     /translation="MKKKSSLKHTGAFWAFCAPCCILFGVFFLVPLVLSIAFSFTNYD
                     GWKTIDFVGLTNYITLFKDSKFYLSLGRTLLYTVCTLPFRVIIPLLVAVLLCSKRVAG
                     KSLTRTMVYIPVLMSSLVVGITINWMFSQEYGLVNFIIRSLGGTALEWSLNPRLATFV
                     IAFASVWASIGFNMILYIGGINSISNDLYEAATIDGASGVQAFFRITVPMLAPTTFMV
                     VLLSTVNLLKEYALVQGITQGGPGLSTTFIIQYIFDKGFNQMQYGYASAISTVVMIIF
                     ALIAFAQFKVNNGGET"
     gene            9634..10458
                     /locus_tag="ACZPAX_02560"
     CDS             9634..10458
                     /locus_tag="ACZPAX_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009322414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR1112811.1"
                     /translation="MKDKRVSIPMTILSLAIGVIVIFPIIWLVLSSFKPSGELFTYPL
                     HMFPSTPTTEHYSSVLAGGFMGYVKNSLFLAVVGTLITLLISAMCGYALAIYRFEIKY
                     VNLVFGVFLLGTLIPGETLTVPQFSVISAMGLYNNIWGVILPVVTTTTGIFMYRQHYM
                     SLPLSLVEAARIDGASEWKIFSNIMLPLGSNVTITLTIFSFMWRWNDYILPLLVLSDQ
                     KKYTIQIAIKNFIGNTGVDWSSILAASVLSILPIVILFMILQKYIVGGVSAGGVKG"
BASE COUNT         2037 a         3344 c         3109 g         2199 t
ORIGIN      
        1 atcgagggcg actattacac cctgcagccc tatgatatcg tattgctgcg cccttccctg
       61 ctgcacaaga ccgagtatcc atcgggcggc gactgccggc ggcttatcat caacttccgc
      121 gtgccggatg attcccccgc gctgcacgaa atgctgcaaa gctgtttaca gcccttcggg
      181 gccgaggtgc ccatctaccg ctttaccggt gagacgcggg ttcaggcatt ctcgcacctg
      241 aacgcgatct tcacgctggg caagcagccg atgaccccgc tgacgcagca gttcatccac
      301 tgccacttcc tgcaatttct ggccaccgtg gcccagcacg cgggcgagag cagctacgag
      361 ccgcaggagc tcagcgattc catcacccag aaggtctacg ccgtgaccag ctacatccac
      421 cggcactacg ccagcgagct ttcgctggaa tatctggcgg agaaattctt catcagtccg
      481 tactatttgt cgcaccagtt ccgccgggtg acagggttct cgctcatcaa ctacatccag
      541 atcacccggg tgcgcaacgc ccagcagctg ttgctgtaca ccgacatgaa aattgccgat
      601 atcacgacga gctgcggctt tacgagtttt tcccagttca accgggtgtt caacaagttc
      661 tgccatacct cgccgtccca gttccgcgtc aacggcgtcg tcacccccat caccgccatt
      721 cccttcgaga aggaaccctc cccggggagc acgaaggtgt aacattttcc ggtgccgccc
      781 cttctcgggg caggcacctt ttttattttt cctgcatcgt cagtttgccg taattgtcaa
      841 aaccactgta ctgcatcggg gcgtcgttta catacagcgt ttcgtcccac tggcagcgca
      901 gttcgccgta gacggggtcc acaaagcggt agcggcggcc ctcggcatcg gcgtccagcg
      961 cagccccgcg catggcggcc ttgaaatcct caaacgccgc aaactcgtct gcctgcgcgg
     1021 cacgcaccag ccagatgcag cggcggccct cggcgatgca ctcgcggtcc ttgttggagc
     1081 cgaagtcctg catccgcatc ccattgctgc aatacaccgc cgcataggca ctgcccagct
     1141 gcacaaaggc ccacttgcct tcgacctcgc agcggtcaaa ctccatcgat ggcaggtaca
     1201 ggtgggtgaa atcgaccggg tgttcggggg cgatatcata gatcagcccc gcgaatgcct
     1261 tgtactggtt gaccttgggc agggtgccat tgcccgccca gtaggacggc cgcgctgcgc
     1321 cgaagagcac ccgctcgccg gggtgggtga cccacagctg cgccacggcg ttgaaggtca
     1381 gctggaaggg gttctcctga tggcccggct caccggggtg gaagtcgttg gcgctggtca
     1441 tcatgtagtc ggcggtcttg taggtgtata gatccacata gccgtcattg ccctgggtcg
     1501 tctggcagat catttccttg ccccggggga tgctgaacca ctgggcaaac tccttcggcg
     1561 gctcgtaatc agacaggcag taggaggtga tacccttgcc cgcgtggccc ggcacgccgt
     1621 acccatagcc gatccagctc aggccggagg ggcagttgga ccagttgccg aactgctcct
     1681 tgatgtaggt gcggcccgac gacgaggcga agatgccctg ctggctgtgg acggccagca
     1741 ggtagtaggc aaaatccatg ccctcgcggc ccagctcccg catggcgggg tcctgggcaa
     1801 aggcatacag gctgccgaag cccagcgtgt caatgggcag gtaggggctg gaattccact
     1861 cggtaaagcc ctctttgcgg aaggtgacga accagtcgta gagcatattc ttggccttgg
     1921 cctgcatctg gcggccggtc agtccgctgt tggtaaagac ctcatcgggg aacagctcgc
     1981 ccgccagcag ctggcaggtg tggaacatca gggcgtggtt ctcgctccag aaccacatgg
     2041 cgtcgttgcc cggctcatcg atccaatagc ggaaacccag cagacagtcg gtcagctcgt
     2101 tctcggtctt ttcggacaaa ataccgctgc cgcgtttgcc gaaggtgcgc aggatgtagg
     2161 ggtagtagac aaggtaaaaa tcgctgcagt cgaagcggcg gcggatgtag tcggactgca
     2221 cggccagcag cttttctgcc tcggcggtgt cgccgccggt ggctaagatt gccagtgcgc
     2281 ggttcgtgtt ctgctcgccc cagcgggcca gcagctccag cgcctggcgc ttgcgctcgg
     2341 ccacggtggg cagggcgcgg ggcagcacgc tctcggtgtg aatttccaca ctgatgggtc
     2401 gggtcagccg gatgccctcg acctcggtcg tgacctgcag cagcaaaaat gccagcggga
     2461 aggtcttgca gtcgcccagg ctgatttggc tcgcgtgggg gagggtgtca gcctcgcggc
     2521 ggaagagcac gccgcccttg acattttcct cggtgccgcc gacaaaggcc aggtggtagc
     2581 tctcggcgat atccgcgccg tcgtagttca gcagcaccgg gccggcggta aagtggttgc
     2641 gatggaacgc caggctgcgc attaggtgct cggcctcggc caggcgggcc gcgtcggcct
     2701 cccccaccgg gatgacctgt tcgggcgcgg tgccctcgcc cttccagcgc aggcgcagaa
     2761 tgatcgtagc atcgcgctcg gcgtagttgt caaagaacac aagaagcttg ttgcggccct
     2821 tttgcagcgg cagggtcagc ggcgtgggga caggctcgtt gtggtggtag ggggtcactt
     2881 cagtcagctt ttcgccgttc agccagaggg ccgcgccgcc gcaggtgata aggtcaaagg
     2941 gcacactgcc cgcctcggcg gcgtccagct cggtgtaagc ccaggcgctc aggcaggcgg
     3001 gggtctcaca gtcccaggcg taggtcacgc cgatatcgtc aaacggccag tagacggcaa
     3061 agggggtagc gttatccgcg ccgcccacca gcccgccggg gcgggtgtcc agctgcgctg
     3121 gccaggcagt ggccttggcg cggcgctcgg ccttgaactg ttcctcgccg ggtttatcct
     3181 tgggggcggt gttcagcttc tcgttttcgg caccgtgcag ggccgtgggc ttggtgatga
     3241 ccggcgtttc ggtcacgccg ctgaccagcc agcggtccac atacccgtga aacaagggaa
     3301 agtgcaattc gtttgccata cttcggcttc tcctttatta aaaaaggttt attaaaaaac
     3361 aacagacgcc ccgcccggtt tgggcggggc gtctgttcgc cctagggaat cacttcccct
     3421 gttgattcgg ttgattagtg tgcccagccg gccttgtcag ccagtgcggt agcaaactcg
     3481 gtcagagcgt cagacggagt caggtcgccg ttgacagact gcttcagagc gtcacggtac
     3541 tcgttgtcgt agtagttgcg ccactcggag ctttcgtcga tggcgaatgc atcggtggtc
     3601 tggccgacct cagcctgcag gaccttgtag tcggcagcct gatcgggatc agcaggctca
     3661 taggtgacgg atttcagaga ggacaggccc ttatcaagtg ccagataatc ggcaaagttc
     3721 ttgtcggtgt agaaccactt caggaactca agagccagat ccttgttctt tgcgttggtg
     3781 ggaacggcca gaccggcacc gcccaggata gcggaaccgc ctgcagaacc gacaggagag
     3841 ggcatgacgc cgaacttgaa gctggtgata tcctgcacga aggggttgta gttccaggag
     3901 ccggagaagt agatgcctac gtcaccgttc ttgaagtagt cgcccgggtt gtcgctggag
     3961 ccaccggccc agatggcctt gggcatgatg ccgctgttgt tggcatcgac aaaggcctgc
     4021 agggcgtcaa tgttctgctg gctgtctgca acgaccttga aggagtcgcc gtccttctcg
     4081 accagagaac cgccgttcat gtacatcagg ttgtcataac gagcgcggga gtaatccata
     4141 gccatgccgt acttgacagc gccggagtcc tgcagcttct tggcagcctc atagacctcg
     4201 tccatggtcc agcggtcgtc caccgtcggg cactcgatgc cggcagcctc aaaagcatcc
     4261 ttgttgtaga atacgttggt gatggtgaac tgctggggca gaacagacag cttatccgtc
     4321 aggtagttct ggctgatgat ctgcagggcc tgcggctcaa agatagaggt gtcaatgcac
     4381 ttgcccagat cctcgaactc atcgggatac agacgagcca gacgggtggt ggacagcaga
     4441 tccggcaggt cgttgttctt ggccatcagg gggaacttgg tcagctggtc atcgtagggg
     4501 atgaccatga tgtcaacggt gttgccggtc tcgtcggccc acttgttcag ggtggtagcg
     4561 aatgcatcgc cctcgccggg ctcctcagac agcatgacct tcagggtctg gccgccggca
     4621 gaagcggcgc cggaagctgc ggtgtccgtt gttgcagcgg agctggccgt agatgcagcg
     4681 ggagaaccgc cgcaggctgc cagagaagcc gccatcgcgg ctgccagagc cagagaaagt
     4741 gcttttttca tgttggtttt acctcctgta aagtagaaat aaaaataggg tatatccacc
     4801 gggccctcac ccggtttgct ccgttccggg gcttgggaac tgggctttca gctcccttgg
     4861 tgtatccata gtgtaccatt gcggcgggtg gattctctac gcctatcttg ctgatttgca
     4921 ttgcggcata ttgcattttt tgcgtctcga tttttcaatc tggcaaattg catcaagaaa
     4981 agcggctgaa attgtgcact tttcacacat cgtttgttct aatgatgcca agttgcgctt
     5041 attttgcgcc ggcgcttatt ttttcagtga aagaaatgca atgtttctgt aaccaaattt
     5101 taataaaaag cagtcgtttt cccccgtaaa caataaatgc gcaccattcg ccctctaccg
     5161 cgcaaaaact gcattgtctg cacagttttg agatgttata ctgtgcttga acaggggttg
     5221 cctgggtgcg ggcgaacaat gttcgcccct acaataccgt tacccgcctt gctgcctcgt
     5281 agggcggcca gtcccctggc cgccgcttcc cttaccacac acttaatctt ataaaaaagg
     5341 aaggagccgc catgcaaaac actgaactcc gccgccgggc gcgcccggtg ctggacgccc
     5401 tgcgcacttt ggcgctgcag gggcgcaccc cggttttggg gctgcgcaaa aaatcctgcc
     5461 cctaccacag ctgggacacc gtgcaggagg acccggccac agcccccggc tggaccccct
     5521 ttgcccccgg tgatgccatc ggcggcgtgg aggcccacta ctgcttccgg ggcggcatca
     5581 ccgcaccggc gggcagcgcc gggcgtcacc ttgtctgcct ggtctccacc ggcgcaaccg
     5641 atatctggaa caacaacaac ccccagttcc tcgcctactt aaatggtgcg ctggtctgcg
     5701 ggctggatgt caaccacacc gagtttgacc taccctccgc cgctgccgag ggccagacct
     5761 ggcagctggg actgtacacc tactgcaaca ccccggctca ggacgtcttt ttaaaagtcg
     5821 agactgccgt gcgggacgat gagatcaccg gcctgtacta cgacctgaaa gccccttacg
     5881 aggtgcttgt ccagctggac gagggcgaca ccaacgccat cggcataggg cggtatctgg
     5941 aaaaagcgct ggatatgctc gacctgcgcg acccgtacag cgccgcgttc tacgccagcg
     6001 tcgccgccgc ccgccgctat atgaaagagg aattttacga gagcttctgc cagcggcagc
     6061 cggtcacggt ggactgtgtc ggccacaccc acatcgacgt ggcctggctc tggacgctgg
     6121 cccagacgcg ggaaaaggcc attcgcagct ttgccaccgt caactacctg atggaccgct
     6181 accccgagta caagttcctg tccagccagc cgcagctcta tgattttgtg cgcaaagact
     6241 gccccgcgct gtttgagaag atccgcgccc gcgtcgccga cggccactgg gaaccggagg
     6301 gcggtatgtg gctggaatcc gactgcaaca tggccagcgg cgagagtttg atccgccaat
     6361 tcctgcacgg gcaggaattt ttcggccggg aattcggcaa acaggggcac atcctgtggc
     6421 tgccggatgc cttcggtttc agcggcgcgc tgccccaaat tatggcccag tgcggcatgg
     6481 actggttcat gactaccaag atggcctgga acgataccaa ctgtatgccc cacgatctga
     6541 cccgctggcg cggcatcgac ggcaccgagg tactggccta ctttatctcc accaaggact
     6601 acgacaagcg ccccgaccgc aacccgaaac cgagcttcaa cacgacctac aacggcctgc
     6661 tgaaccccag gcaggtcatg ggctgctggc agcgctttca ggacaaggag ctgacccagg
     6721 atgtgctgca atgctatggc tacggcgacg gcggcggcgg tgtgaccgct gaaatgctgg
     6781 agatcaaccg ccgcatggag aagggcatcc ccggtgcgcc ccagacccgc ctgacccacg
     6841 cggcccccta ctttgacaaa ttgaagcagc accttgacga gacgcaggcc gacctgccct
     6901 gctggcacgg cgagatgtac tttgagtacc accgcggcgt gttcaccagc cagggccgca
     6961 acaagcgggc caaccgcgcc gccgagtttg ccaacctgac cgccgagacc gccgctgccc
     7021 tggccgaaag tctgcgcacc ggctacgcct accccgccgc cgcgctgcac cgcaactggg
     7081 agctgaccct gctgaaccag ttccacgaca ttttacccgg ctcggccctg ggcgaggtat
     7141 acgaagtctc gcagcagcag tacggcgaga tcctcgcgtc cgatgccaga attaccgatg
     7201 acgcgctgca caccgtcgcg gccctcgtca agaccgaccg ccccggcgtg gtcgtgttca
     7261 accagctggg ctttgcccgg gacaccgtcg tccgcgtcgc ctgcggggca agcggcatca
     7321 ccgacggcgg acacccgctg ccctgccata ccgaaaacgg cgcgctgacc tttgttgcca
     7381 aggacctgcc cgccaagggc tggcggttct acccctttgc cgacgcagaa cccgagaccc
     7441 cctgcgccga agtcgccgaa aacgacggcg gctatatcat tgacacgccg ctctatcgca
     7501 tcgtattcaa cggctgcggc gagatcaccg ccctgctgga caaggaggcc gaccgcgagc
     7561 tgatccctgc cgggcagtgc gccaacgaac tgcagctgtt tgaggaccgc cccgacgagt
     7621 acgacgcctg gaacctggaa aaatactacc gccgccaccg ctttgcgctg gacggtgcgg
     7681 cccgcctgac tgtggccgag aacagcccgg tctgctgccg cctgcatctg gaacgcgcca
     7741 tttcccggtc gcagctcgtg caggatatca ccctctaccc ccacagccgc cgcatcgact
     7801 ttgccaccca catcgactgg cacgagcagc atgtgctgct gaaagccgcg ttcccggtgg
     7861 atatctgcgc cgaccgcgcc cagtatgaca tccagttcgg cagcattgcc cgggataccc
     7921 acaccaacac cagctgggac gaggcccgct tcgaggtctg cgcccataag tgggccgacc
     7981 tgtccgagcc gggctacggc gcggcgctgc tgaatgacgg ccgctacggc tatgatgtcc
     8041 acgacggcgt gctgcggctc tcgctgctgc gggccgccac ctacccggac ccccacgccg
     8101 accagggggc ccacgacttt acctatgcgc tgctgcccca cctgggcgac tggcggcagg
     8161 gcggggtcat ccccgccggg tacgacctga acgccccggc gctgcctctg gccgtggccg
     8221 cccaacccgc gggcacgctg cctgctgcgt acagccagtt ccgcgtagat gcccccaatg
     8281 tcgtgttgga gaccgtgaag aaagccgagg atggctccgg cctgattttg cgcctgtatg
     8341 agagctgggg cacccgcacc cgcgcggcgt tggccctgcc ggatgatgct gctgccgtgc
     8401 gctgcacgcc gatggaacag cccttacccg gagaagaagc cgtgcagcac agcctgccgc
     8461 tgacgctgca tccctttgaa atttgcacaa tccatgtaca atttgcgcag tgacattcac
     8521 tgtggatgca tgattttaca gtttgcacaa tttcacagca ttgcatttgt acgttttcgc
     8581 caagacgacg gtcagatttt gcgccgcctg ccaaaatcgc gcaaaaaatg caatgcattt
     8641 tgggacaagc aagcaaaaaa acggtggtat ttgcgccttt tgcaatgcta tactaagcat
     8701 atcgaaggaa ccgggcggtg gaatcccggc caaagagaat aaaggaggaa tgaatatgaa
     8761 gaagaaatcg tcgctgaagc acaccggcgc attctgggcg ttctgtgcgc cctgctgcat
     8821 tttgttcggt gtctttttcc ttgtgccgct tgtgctcagc atcgcttttt ccttcaccaa
     8881 ctatgacggt tggaaaacca ttgactttgt gggtctgaca aactacatta cattgtttaa
     8941 agactccaag ttctatctgt ccctggggcg gacgctgctg tacacggtct gcacgctgcc
     9001 gttccgcgtc atcatcccgc tgctggtggc ggtactgctc tgctctaagc gcgttgccgg
     9061 caagagcctg acccgcacga tggtctacat ccccgttctg atgagttcgc tggtcgtcgg
     9121 tattaccatc aactggatgt tcagccagga gtacggcctt gtaaacttta tcatccgttc
     9181 gctgggcggc accgcgctgg aatggtcgct gaacccgcgt cttgccacct ttgtcatcgc
     9241 gttcgcatcg gtctgggcgt ccatcggctt caacatgatc ctctacatcg gcggtatcaa
     9301 cagcatttcc aatgatctgt atgaggctgc taccatcgac ggtgccagcg gtgtgcaggc
     9361 cttcttccgc atcacagtcc caatgctggc ccccacgacg ttcatggtcg tgctgctgtc
     9421 caccgtcaac ctgctcaaag agtacgccct ggtccaaggt atcacgcagg gcggccccgg
     9481 cctgagcacc acctttatca tccagtacat cttcgacaag ggcttcaacc agatgcagta
     9541 cggttatgct tcggccattt ccaccgttgt catgatcatc tttgccctga ttgcctttgc
     9601 ccagttcaag gtcaataacg gaggtgaaac ctgatgaaag acaagcgcgt cagcattcca
     9661 atgacgatcc tttctctggc aatcggcgtc atcgtgattt tccccattat ctggctggtg
     9721 ctcagctcct tcaaaccgtc cggcgagctg ttcacctacc cgctgcatat gttcccctcc
     9781 accccgacga cggagcatta ctcctctgtc ctggcaggcg gcttcatggg ctatgtgaag
     9841 aacagcctgt tcctggccgt ggtcggcacc ctcatcacac tgcttatcag cgccatgtgc
     9901 ggttatgcgc tggccatcta ccgctttgag atcaagtacg tcaacctcgt gttcggcgtg
     9961 ttcctgctgg gcaccctgat ccccggtgag acgttgaccg tcccccagtt cagcgttatc
    10021 agcgccatgg gcctgtacaa caacatctgg ggcgtcatcc tgcctgttgt caccaccacg
    10081 accggtatct tcatgtaccg ccagcactat atgtcgctgc cgctgtctct ggtcgaggct
    10141 gcgcgtatcg acggtgccag cgagtggaag atcttttcca acatcatgct gcccctgggc
    10201 tccaatgtca ccatcacgct gacgatcttc agcttcatgt ggcgctggaa cgactacatc
    10261 ctgccgctgc tggttttgtc tgaccagaag aagtacacga tccagattgc catcaagaac
    10321 ttcatcggca acaccggcgt ggactggagc tcgatcctgg cggcatcggt tctgtccatc
    10381 ctgcccatcg tcatcctctt catgatcctg caaaaataca ttgtcggcgg tgtctccgcc
    10441 ggcggcgtca aggggtaaga ttccctctct cttgccgcag ctgccctccg gtttgccggg
    10501 gggcattttt tgaacgatgc agaaagggcg cggtgcgtcc tcccggcagg ggccgggcac
    10561 gcccggcccg cagccctgcc atcactgcat atttaccctt acacaaggag gtttccccca
    10621 tgaaatacgg ctgccgcgcc catgattacg ggtgctttac cgccaaaggt ctggccgatg
    10681 tgctgaccg
//
LOCUS       DBMDSQ010000038        10038 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_1943_length_10038_cov_1.752239, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000038 DBMDSQ010000000
VERSION     DBMDSQ010000038.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 10038)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 10038)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10038
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1943_length_10038_cov_1.752239"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1265)
                     /locus_tag="ACZPAX_02565"
     CDS             complement(<1..1265)
                     /locus_tag="ACZPAX_02565"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005945387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HIR1112812.1"
                     /translation="MKNSEKTLDMIVNLCKNRGFIYPGSEIYGGLANSWDYGPLGVEF
                     KNNVKKAWMKKFVQESPYNVGLDAAILMNPQTWVTTGHVSSFSDPLLDCRACKSRHRA
                     DKLISECEQGKGVDVDAMTFDEMDAFIASHDEVVCPVCGKHDFTPIRKFNLMFKTAIG
                     VTEDSSSTCYLRPETAQGIFVNFANIQRTTRRKLPFGVCQVGKAFRNEITPGNFTFRT
                     REFEQMECEFFCKPNTDLEWFAYWKDYCKNWLLSLGIKEENLRLRDHEPAELAFYSRA
                     TTDIEYAFPFTDWGELWGIADRTNYDLSRHQEASGKSLEYFDPETNEHYIPYVIEPSL
                     GCDRVALAFLCEAYDEQHLVDAKGKEDVRTVLHLHPYLAPFKCAVLPLSKKLGDKAME
                     IRNELAKDFMVDYDDAGSIGKRYRRQDEIG"
     gene            1649..3019
                     /gene="murC"
                     /locus_tag="ACZPAX_02570"
     CDS             1649..3019
                     /gene="murC"
                     /locus_tag="ACZPAX_02570"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HIR1112813.1"
                     /translation="MVFSMYDPKLLEGVRTIHFIGCGGSGTYPLIQILHSRGYAITGS
                     DVEETKNTEAERALGVRVSIGHDAANVGNADLVVYSAAIHDDNPELQAARARGIKAIE
                     RSVMLGYISRTHSQSIGVAGTHGKTTTTGMITTMLELAGKDPAAVIGGKLPLIGGYGK
                     AGNGQSVVIEACEYHETFLQLTCSVGVILNIDNDHLEYYGTMGQLKLAFQKFALLSRT
                     VVFNMDDKNTMDVVNSIDRPVLSFGIKEEARFRAVNIGEYKPGFFEFDVLELGEQFAH
                     IKLGVPGYHNIYNALAMCCCVRPLGLKPEDAVRAAEQFHGTGRRFEVKGECNGAVIVD
                     DYAHHPTELAATLATAKEMGYDRVIAVHQPFTFSRTKMLMDEFAKVLSAADQVVLLPI
                     MGGREKDDGTVKSEDLAAKLPGSVVVDGLEGAANWVKQNAKKGDLVVCMSCGDLYKAA
                     DMMAGK"
     gene            3268..4503
                     /locus_tag="ACZPAX_02575"
     CDS             3268..4503
                     /locus_tag="ACZPAX_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HIR1112814.1"
                     /translation="MNQHFLQLYADFIVKVGVNVRPRQNFIVRCPVTMPDFAHACVRA
                     GYEAGAKTVVVRWEDDKLTRLNMEMADEKDLAAMKPYELRSYLDYAEDPDGCCTLAIH
                     AEDPEALTGLDAGKLNRVNLARRNFLKPWQEYTMNDRVQWCVAAVPAPGWAAKVFPEL
                     PIDEAVEKLWALIFDVCRVSTGDPVAAWQEHVAKTKARRDHMNELDLDRIHMTSANGT
                     DLTVGLADDATWEGASSKTEGGIEFIANVPTEEVFCAPHRERTNGIVYGTKPYAYNGQ
                     LIEGWHVTFKDGVVVEHGAEKNADLLTELLSTDENSNRIGEIALVPASSPINRSGVLF
                     FNTLFDENAACHIAFGAGYPTNIKGGSAMTRDELMKKGLNNSAIHEDVMIGAADTHVT
                     GITKSGEEVAIFENGEWAF"
     gene            4491..5663
                     /locus_tag="ACZPAX_02580"
     CDS             4491..5663
                     /locus_tag="ACZPAX_02580"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HIR1112815.1"
                     /translation="MGVLNRALAPIDTDRPAAADFVRVLATWAVGAFHIWQQSWYGGA
                     APAHWLRAGSVGVDLLVMLSAFCLFLPWANAAVRNQPLPRIRPAEFYRRRALRLLPAY
                     YASLLFSLVLAVARRGWSKALALDLAAHLALTQQLYRPAYLACQLNGVTWTLTVFALF
                     YLVFPLLASLCAKWPLPTLAALGLVQLGYTCWLLPQYGSGDYSARFNQLPAFSGVLAV
                     GLAAAWVFAKFAHGGWAQHVLPRAGCTALGLAALIWLDAEMRAQAAAAEYQQFQLINR
                     MPLVLAAAAVLVCFGLGLTLPEVLRRALRWLAALTYSFYLWHQMLTVFLKYDLHLPAW
                     AGDTPPNQFGDAAWMHRADLLYWAAALAVSAAAYYLIEKPAANFIATHKTVSQPRM"
     gene            5682..6614
                     /locus_tag="ACZPAX_02585"
     CDS             5682..6614
                     /locus_tag="ACZPAX_02585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1112816.1"
                     /translation="MKKTVLTVLALAVLLTGCAKGADTTDTTNNVQMRVQDGYIQYYN
                     GTDWQNLVSTDELKGEKGDKGDKGDQGEQGVHGERGPAGTSTGTAGATGATGATGAQG
                     PKGDKGDKGDKGDTGAQGPKGDKGDKGDKGDTGAQGPKGDKGDKGDKGDTAEVTRQFQ
                     IFVAKDNTLQYQTKDVVEVVDSSDAELFTDTDDNGKTNYTGFALKENGWVDVKVKSQS
                     GDTFAGWSDGEKKETRRITYSDATILFANYWTQTPTTATPVPSTPVPTAPAIPTPEPT
                     AIPEPTAAPTPIKKPDSAPTEAASELKRPTGKGK"
     gene            6816..7310
                     /locus_tag="ACZPAX_02590"
     CDS             6816..7310
                     /locus_tag="ACZPAX_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020074323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HIR1112817.1"
                     /translation="MAKPGKPQQGSAVKVVEALVRPVVEGMNLRLWDVRFEKEGPDWF
                     LRVLIDRDEPLDTDTCEAVSRAIDPLLDEADPIEQSYYLEVGSPGLGRRLTRPEHYEQ
                     LKGQKCAAHLIRPDEQGRRDYAGILQGLDADGNVTLTDGEESWTFPVKSAGYVKLCDD
                     EDLF"
     gene            7394..8467
                     /gene="nusA"
                     /locus_tag="ACZPAX_02595"
     CDS             7394..8467
                     /gene="nusA"
                     /locus_tag="ACZPAX_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HIR1112818.1"
                     /translation="MATANNEFFEALAALEKERGLPADYLIEKIKAAIVIAVKKDYEV
                     EDDNVVVDIDPELGAFRASLLRDIVEEVENPHTQVSLEDAQKIRKSYKVGQRMVTPLK
                     TKEFGRIAAQTAKHVIRQGLREGERNLQCSEMQSRAHELIAATVVSIDPERGNIVLDL
                     GKGGSAVLPRNEQVPGETFTEGQTVQVYVVDVLATDRGPRVTISRTHPGLVKRMFELE
                     VPEIYDGTVEVKAIAREAGARTKLAVWSKNPDVDPVGACIGARGARVEKIVQELNGEK
                     IDVIRWSEDITEFISAALSPAKVVKVELLPGETKSCRVTVPDHQLSLAIGNKGQNVRL
                     CAHLTGYNIDIRPESGYYGEDEE"
     gene            8619..8927
                     /gene="rnpM"
                     /locus_tag="ACZPAX_02600"
                     /gene_synonym="ylxR"
     CDS             8619..8927
                     /gene="rnpM"
                     /locus_tag="ACZPAX_02600"
                     /gene_synonym="ylxR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase P modulator RnpM"
                     /protein_id="HIR1112819.1"
                     /translation="MQQQKQKKIPVRRCVGCNAQRPKRELVRVVRSPEGEISIDLRGK
                     APGRGAYLCPSAACLAKARKAKRLERTFEVPIPDGIYDRLTEEIQCAEQSAKEAASDE
                     "
     gene            8920..9222
                     /locus_tag="ACZPAX_02605"
     CDS             8920..9222
                     /locus_tag="ACZPAX_02605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="L7Ae/L30e/S12e/Gadd45 family ribosomal protein"
                     /protein_id="HIR1112820.1"
                     /translation="MNNDQHPLLGALGLCRKAGKLLHGYDRVQENALRGKVALVLLTS
                     DASERTVSHMKDACDGIVACEQMPLTSADLAMLTPKPAAVFGVTDEHLAQLCAKYL"
     gene            9241..>10038
                     /locus_tag="ACZPAX_02610"
     CDS             9241..>10038
                     /locus_tag="ACZPAX_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HIR1112821.1"
                     /translation="MDFKYKIADVAKEFGVPAKKVIETVSGVTGTAYKTGGTFEEKEL
                     NVLLETLTRENAETSLDAYLASGKEEAKPAPKAEPKKPEAKKAEEKKPAAQPAKAEQP
                     KAEAKPEQKPEQKKDRKPEKQAEKRNEKRVTLQELAADTGIKEPVKTEQVQVNRAQVS
                     VDTRTVDVNVDKFNARYDDLADSRNMPSKRKNQPTGKKEKFNNRNNRRGQQFGRRRET
                     EAERLQRIQLEKARNAQLKISIPDEITVGELATRLKQNAAKVVAKFMQ"
BASE COUNT         2181 a         3055 c         3026 g         1776 t
ORIGIN      
        1 ccgatctcgt cctggcggcg gtaacgcttg ccgatggaac cggcgtcgtc gtagtccacc
       61 ataaagtcct tagccagctc gttgcggatc tccatcgcct tgtcgcccag ctttttggac
      121 aggggcagca cggcgcactt gaacggtgcc aggtaggggt gcagatgcag cacggtacgc
      181 acatcctcct tgcccttggc atcgacaagg tgctgctcat cgtaggcctc gcacaggaaa
      241 gccagcgcca cgcggtcaca gcccagagac ggctcgataa cataggggat gtaatgctcg
      301 ttcgtctcgg ggtcaaagta ctcgagcgac ttgccggagg cctcctgatg gcggctcagg
      361 tcgtagttgg tgcggtcggc aatgccccac agctcgcccc agtcggtgaa cgggaaggca
      421 tactcaatgt cggtggtggc gcgggagtag aaggccagct cggcgggctc atggtcgcgc
      481 aggcgcaggt tctcttcctt gatccccaag ctcagcagcc agttcttgca gtagtccttc
      541 cagtaggcga accactccag atcggtgttg ggcttgcaga agaattcgca ctccatctgc
      601 tcaaactcgc gggtacggaa ggtgaaattg cccggggtga tctcgttgcg gaacgccttg
      661 cccacctggc agacgccgaa gggcagcttg cggcgggtgg tgcgctggat gttcgcgaag
      721 ttgacgaaga tgccctgcgc ggtctcgggg cgcagatagc aggtggagga ggaatcctcg
      781 gtaacaccga tggcggtctt gaacatcagg ttgaatttac ggatgggggt aaagtcatgc
      841 ttgccgcaga cggggcagac gacctcatca tggctggcga taaaagcatc catctcatca
      901 aaggtcatgg catccacatc cacgcccttg ccctgctcac actcggagat cagcttatca
      961 gcgcggtgac ggctcttgca ggcgcggcag tccagcaacg ggtccgagaa gctggacaca
     1021 tggccggtgg tgacccaggt ctgggggttc atcaggatgg cggcgtccag gccgacattg
     1081 taggggcttt cctgcacgaa cttcttcatc catgcctttt tgacgttgtt cttgaactca
     1141 acacccagcg ggccgtagtc ccagctgttt gccaggccgc cgtaaatttc gctgcccgga
     1201 tagatgaacc cgcggttctt gcacaggttc acgatcatgt caagggtctt ttcactgttc
     1261 ttcattacgt tactccttcc gcatggctgg ctgccacaca aagcgattat tcccaccgcg
     1321 ccggataaat ttggtgcgat ataaccatta tagcatacca tgcgggcggg aacaaggcac
     1381 tgcagcaaaa atatgtctgc gggtgcggcg tagtttatcg gacacacaaa aaccttttgc
     1441 cgcgtcggaa agactgctgc aactgcacca tcaacgtatt gctgtaattt atgggtctaa
     1501 atttgtgcca tttacccaaa aattgcccgg tcaggcatcg aattcaaaaa aagtgtttat
     1561 tttgttgcag agaaatagta taatgtttcc agtaatatac gccgcgtgcc ctgcccggca
     1621 gcggataaat tttgaggtga ccactgttat ggtttttagt atgtatgacc ccaagctgct
     1681 ggaaggcgtc cgcacgatcc attttatcgg ctgcggcggt tccggcacct atccgctgat
     1741 ccagatcctg cacagccgcg gctacgccat caccggctct gacgtcgagg agaccaagaa
     1801 caccgaggcc gagcgtgccc tgggcgtgcg cgtctccatt ggccacgatg ccgccaatgt
     1861 cggcaacgct gatctggtcg tgtacagcgc cgccatccac gacgacaacc ccgagctgca
     1921 ggccgcccgc gcccgcggca tcaaggccat tgagcgcagc gttatgctgg gctacatcag
     1981 ccgcacccat tcccagagca ttggcgtggc cggtacccac ggcaagacca cgaccaccgg
     2041 catgatcacc accatgctgg aactggcggg caaggatccc gctgcggtca tcggcggcaa
     2101 gctgcccctc atcggcggct acggcaaggc cggcaacggc cagagcgtcg tcattgaggc
     2161 ctgcgagtac cacgagacgt ttttgcagct gacctgctcg gtgggcgtca tcctgaacat
     2221 tgacaatgac caccttgaat attacggcac gatgggtcag ctcaagctcg cgttccagaa
     2281 gtttgcgctg ctgtcccgca cggtcgtctt caacatggac gataaaaaca cgatggacgt
     2341 tgtcaactcc atcgaccgcc cggtgctgag cttcggcatc aaggaggagg cccgcttccg
     2401 cgccgtcaac atcggcgagt acaagcccgg cttcttcgag ttcgacgtcc tcgaactggg
     2461 tgagcagttt gcccacatca agctgggcgt gcccggctac cacaacattt acaatgccct
     2521 ggctatgtgc tgctgtgtcc gcccgctggg cctcaagcct gaggatgccg tccgcgcagc
     2581 cgagcagttc cacggcacgg gccgccgttt tgaggtcaag ggcgagtgca acggtgcggt
     2641 catcgtcgat gactacgccc accaccccac cgagctggcc gccacactgg cgactgccaa
     2701 ggagatgggc tatgaccgtg tgatcgccgt acaccagccc ttcaccttca gccgtaccaa
     2761 gatgctgatg gacgagttcg ccaaggtgtt gagcgccgct gaccaggtcg tgctgctgcc
     2821 catcatgggc ggtcgcgaga aggacgacgg cacggtcaag agtgaggacc tggccgccaa
     2881 gctgcccggc agcgttgtgg tcgatggcct ggaaggcgct gcaaactggg tcaagcagaa
     2941 cgccaagaag ggcgacctcg tcgtctgcat gagctgcggc gatctgtaca aggccgccga
     3001 tatgatggca ggaaaataaa atctgatgac aggctgtccg gcagggcagc ctgttttttg
     3061 caacagagtg caggggccgg gcatgcccgg cccggtcgtg taaggtgagc ggctgtctgt
     3121 gggaaagccg cgggccacac atatgcggcc cttacaacgc tgtgccgccg tagggcagca
     3181 tgccctcatg ccgccgccaa cttgccagta ccacccactt tatgctacaa tatcagcaaa
     3241 caaacattat ataaaggaga tcaccccatg aaccaacact tcctccaact ctacgctgat
     3301 tttatcgtca aggtgggcgt caatgtgcgc ccgcgccaga actttatcgt ccgctgcccg
     3361 gttacgatgc ccgactttgc ccatgcctgc gtccgcgcgg gatatgaggc gggcgcaaaa
     3421 accgttgttg tccgctggga ggacgacaag ctcacccgac tgaacatgga aatggccgac
     3481 gaaaaagact tggccgccat gaagccctat gagctgcgca gctacctcga ctatgctgag
     3541 gacccggacg gctgctgcac gctggccatc cacgccgagg accccgaggc gctgaccggt
     3601 ctcgacgcgg gcaagctgaa ccgcgtcaac ctggcccgcc gcaacttcct caagccctgg
     3661 caggagtaca cgatgaacga ccgcgtgcag tggtgcgtgg cggctgtgcc cgccccgggc
     3721 tgggccgcca aagtattccc cgaactgcct atcgacgagg ctgttgaaaa actgtgggca
     3781 ctgatttttg acgtctgccg cgtctccacc ggcgacccgg tcgccgcatg gcaggagcac
     3841 gtcgccaaga cgaaggcccg ccgcgaccac atgaacgagc tggatctcga ccgcatccac
     3901 atgaccagcg ccaacggcac cgacctgacc gtcggcctgg ccgacgacgc cacctgggag
     3961 ggtgcatcca gcaagaccga gggcggcatt gagtttatcg ccaacgttcc caccgaggaa
     4021 gttttctgcg cgccccaccg tgagcgcacc aacggcatcg tctacggcac caagccctac
     4081 gcctacaacg gccagctcat cgaaggctgg cacgtcacct tcaaggatgg tgtcgttgtc
     4141 gagcacggcg cggagaaaaa cgccgacctg ctgaccgagc tgctctccac cgatgagaac
     4201 tccaaccgca tcggcgagat cgccctcgtg cccgcatcca gccccatcaa ccggagcggt
     4261 gtgctgttct tcaatacgct gtttgatgag aacgccgcct gccacattgc ctttggcgcg
     4321 ggttatccca cgaacatcaa gggcggctcc gccatgaccc gcgacgaact gatgaagaag
     4381 ggcctgaaca actctgccat ccatgaggac gtcatgatcg gcgcggcgga tacccatgtc
     4441 accggcatca cgaagagcgg ggaagaggtc gcgatctttg aaaacggcga atgggcgttc
     4501 taaaccgcgc ccttgcgccc atcgacacag accgcccggc ggcagcggac tttgtgcggg
     4561 tgctggcgac ctgggccgtc ggcgcgttcc acatctggca gcagagctgg tacggcggcg
     4621 cagccccggc acactggctg cgggcgggca gcgtcggcgt ggacctgctg gtcatgctgt
     4681 cggcgttctg cctgttcctg ccctgggcca acgcggctgt gcggaaccag cctttgccgc
     4741 gcatccgccc ggcggaattc taccgccgcc gcgccctgcg gctgctgccc gcctactacg
     4801 ccagcctgct gttcagtctg gtgctggccg tggcgcggcg tgggtggagc aaggcgctgg
     4861 cgctggatct ggccgcccac ctggcgctga cccagcagct ctaccgcccg gcctacctgg
     4921 cctgccagct caacggcgtg acctggacgc tgacggtgtt tgcactgttt tatctcgtgt
     4981 tcccgctgct ggcctcgctc tgcgcaaaat ggccgctgcc cacgctggct gcgctgggac
     5041 tggtgcagct ggggtacacg tgctggctgc tgccgcagta cggcagcgga gactattctg
     5101 cccggttcaa ccagctcccg gcgttcagcg gggtgctggc cgtgggactg gcggcggcat
     5161 gggtgtttgc taaattcgcc cacggcggct gggcacagca cgttctgccc cgcgcgggct
     5221 gtacggcgct ggggttggcg gcgctcatct ggctggatgc cgaaatgcgc gcccaggctg
     5281 cggcggcaga gtaccagcag ttccagctga taaaccggat gccgctggtt cttgcggctg
     5341 cggcggtact ggtgtgcttt ggcctgggcc tgaccctgcc cgaggtgctg cgccgcgccc
     5401 tgcgctggct ggctgcgctg acgtacagct tttacctgtg gcaccagatg ctgacggtct
     5461 ttttaaagta tgatctgcat ctgcccgcct gggcggggga cacaccgccc aaccagtttg
     5521 gggacgctgc ctggatgcac cgggccgacc tgctctactg ggcggccgcg ctagcggtaa
     5581 gcgctgcggc gtattatctg atcgaaaagc ccgccgcaaa ttttattgcc acccataaaa
     5641 ctgtatccca accccggatg taacaaaaag gaggattgac catgaaaaag accgtattga
     5701 ccgtattagc gctggcggtg ctgctgacag gctgcgccaa gggcgcggac accaccgaca
     5761 ccacgaacaa cgtccagatg cgtgtacagg atgggtacat ccagtactac aacggcacag
     5821 actggcagaa tctggtcagc accgatgagc tgaagggtga aaaaggcgac aagggtgata
     5881 agggcgacca aggcgagcag ggtgtgcatg gcgagcgcgg cccggcgggt acctccaccg
     5941 gcacggccgg ggccacgggt gccacaggag ccaccggcgc gcaaggcccc aagggcgata
     6001 agggagacaa aggtgacaag ggcgacaccg gcgcgcaagg ccccaagggc gataagggag
     6061 acaaaggtga caagggcgac accggtgcac agggccccaa gggcgataaa ggtgacaagg
     6121 gtgacaaggg cgacaccgcc gaggtgaccc gtcagttcca gatctttgtg gcgaaggaca
     6181 ataccctgca atatcaaacg aaggatgttg tggaggtcgt cgattcctcc gatgcagagc
     6241 ttttcacaga cacggatgac aacggaaaaa caaactacac cgggttcgcg ctgaaagaaa
     6301 atggttgggt cgatgtaaag gtaaaatccc agagcggtga tacctttgca ggttggagcg
     6361 acggcgagaa aaaggaaacc cgccgcatta catacagcga cgccacgatt ctgtttgcaa
     6421 attattggac tcagacaccg acaacggcaa cgcctgtacc gtcaacccct gtgccgaccg
     6481 caccggctat tccaacccct gagccaacag caatccctga accgacggcg gcacctacgc
     6541 cgataaagaa accagattct gcgccgacag aagctgcgtc tgagctgaag cgccccaccg
     6601 gcaagggaaa ataaccccct ccgccgcccc ggatgggccg tttttccaaa actccgcaaa
     6661 aaatgtgcgc tcaaaaagcc cgataacagg caattccgcc cggcggggac ttgcattttg
     6721 cgtgggagta tggtatattc atagtgttcg atgatgattt tactgaaagt gggccgcaag
     6781 gtccgctttc tttttatgat tggagcattt taattatggc aaaacccggt aaaccgcagc
     6841 agggcagtgc ggtcaaggtc gtggaagcac tcgtccgccc ggtcgtggaa ggcatgaacc
     6901 tgcgtctgtg ggacgtccgc tttgaaaagg aaggcccgga ctggttcctg cgcgtgctga
     6961 tcgaccgcga cgagccgctg gacaccgaca cctgcgaggc tgtctcccgc gccatcgacc
     7021 cgctgctgga cgaggcagac cccattgaac agagctatta tcttgaggtc ggcagccccg
     7081 gcctgggccg ccgcctgacc cgccccgagc attacgagca gctcaagggc cagaagtgcg
     7141 ccgcgcacct gatccgcccc gatgagcagg gccgccgcga ttacgcgggc attctgcagg
     7201 ggctggacgc cgacggcaac gtgaccctga ccgatggtga ggaaagctgg accttcccgg
     7261 tcaagagcgc gggctatgtc aagctctgcg atgatgaaga tttgttctga atggcttccc
     7321 ccaaggggga aggcagagca ttttaaatac tgtattgcgt gtacttgaaa tataattggg
     7381 aggaaaccat aaaatggcta ctgctaacaa cgaattcttt gaggctcttg ctgcgctgga
     7441 aaaggaacgc ggcctgcccg cggactacct gatcgaaaag atcaaggcgg ccatcgtcat
     7501 cgcggtcaag aaggactacg aggtcgagga cgacaacgtc gtcgttgata tcgaccctga
     7561 gctgggcgct ttccgcgcca gcctgctgcg tgacatcgtg gaggaggtcg agaaccccca
     7621 cactcaggtc agcctggaag atgcccagaa aatccgcaag agctacaagg tcggccagcg
     7681 catggtcacc ccgctgaaaa ccaaggagtt cggccgtatc gctgcccaga ccgcaaagca
     7741 cgtcatccgt cagggcctgc gcgagggcga gcgcaacctg cagtgcagcg agatgcagtc
     7801 ccgtgcccat gagctgatcg ccgccaccgt cgtttccatc gaccccgagc gcggcaacat
     7861 cgtcctcgac ctgggcaagg gcggcagcgc cgttctgccc cgcaacgagc aggtccccgg
     7921 cgagaccttc accgagggcc agactgtgca ggtctacgtt gtggacgtgc tggcgacaga
     7981 ccgcggcccc cgcgtgacca tcagccgcac ccatcccggc ctggtcaagc gtatgtttga
     8041 gctggaagtc cccgagatct acgacggcac cgtcgaagtc aaggccatcg cccgcgaggc
     8101 cggtgcccgc accaagctgg ccgtctggag caagaacccc gacgttgacc cggtcggcgc
     8161 ctgcatcggc gcccgcggtg cccgcgtgga gaaaatcgtt caggaactga acggcgagaa
     8221 gatcgacgtc atccgttgga gcgaggatat caccgagttc atctccgctg cactgtcccc
     8281 ggccaaggtc gtcaaggtcg agctgctgcc cggcgagacc aagagctgcc gcgtgaccgt
     8341 gcccgatcac cagctgagcc ttgctatcgg caacaagggc cagaatgtgc gtctgtgcgc
     8401 ccatctgacc ggctacaata ttgatatccg ccccgagtcc ggctactacg gagaggacga
     8461 agaataaata aaaagagata agagataaaa cataataaat aataaaaaat gtggagtttt
     8521 tgcccgcagg gcaaaaacaa aattttgagc ggtgcgcagc gccgcggaac accatatttc
     8581 ttatctttta tctattattt agaaatgagg tctgccagtt gcagcagcaa aagcaaaaaa
     8641 agataccggt gcgccggtgt gtgggctgta atgcccagcg ccccaaacgc gaattggtgc
     8701 gcgtggtgcg cagcccggaa ggcgagatca gcattgatct gcgcggcaaa gcaccggggc
     8761 gcggcgcgta tctgtgcccc tcggccgcct gccttgccaa agcgcgcaag gccaagcggc
     8821 tggaacgcac gtttgaggtt cccatcccgg acggcatcta cgaccgcctg accgaggaaa
     8881 tacaatgtgc ggaacaaagc gcaaaggagg cggcgagcga tgaataacga tcagcacccg
     8941 ctgctgggtg cgctggggct gtgccgcaag gccggtaagc tgctgcatgg gtatgaccgc
     9001 gtacaggaaa atgcactgcg cggcaaagtg gccctggtgc tgcttacctc cgacgccagc
     9061 gagcgcacgg tcagccatat gaaggacgcc tgcgacggca tcgtcgcctg cgagcagatg
     9121 cccctgacca gcgctgacct ggccatgctt acccccaaac ccgccgccgt gttcggcgtt
     9181 accgacgaac accttgcaca gctgtgcgca aaataccttt gatgaggagg accacgatat
     9241 atggatttta aatacaaaat cgctgatgtt gccaaggagt tcggcgtacc cgctaaaaag
     9301 gtcatcgaaa ccgtttccgg tgtgaccggc accgcctaca agacaggcgg cacgtttgag
     9361 gaaaaagaac tcaacgtcct gttggagacc ctgacccgcg agaacgctga gacgagcctg
     9421 gatgcctacc ttgccagcgg caaggaggaa gccaagcccg ccccgaaggc ggaaccgaag
     9481 aagcctgagg ccaagaaggc agaggagaag aagcccgctg cccagcccgc caaggcagag
     9541 cagcccaagg ccgaagccaa gcccgagcag aagcccgagc agaagaagga tcgcaagccc
     9601 gaaaagcagg ccgagaagcg caatgaaaag cgcgtcaccc tgcaggagct ggccgccgat
     9661 accggcatca aggagcctgt caagaccgag caggtccagg tcaaccgcgc ccaggtgtct
     9721 gtggacaccc gcaccgtcga tgtgaacgtt gacaagttca acgcccgcta tgacgacctg
     9781 gccgacagcc gcaatatgcc cagcaagcgc aagaaccagc ccaccggcaa aaaagagaag
     9841 ttcaataacc gcaacaaccg ccgcggccag cagtttggcc gccgccgcga gaccgaggcc
     9901 gagcgcctgc agcgcattca gctggaaaag gcccgcaacg cgcagctgaa aatctccatc
     9961 cccgacgaga tcaccgtcgg cgaactggct acccgcctga agcagaacgc tgccaaggtc
    10021 gttgccaagt tcatgcag
//
LOCUS       DBMDSQ010000039         9722 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_1991_length_9722_cov_1.886186, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000039 DBMDSQ010000000
VERSION     DBMDSQ010000039.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 9722)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9722)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9722
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1991_length_9722_cov_1.886186"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..408
                     /locus_tag="ACZPAX_02615"
     CDS             <1..408
                     /locus_tag="ACZPAX_02615"
                     /inference="COORDINATES: protein motif:HMM:NF013962.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HIR1112822.1"
                     /translation="ASGLHYKIGSINLRGAHPNTAVLRLVGVSGRKLEVVGASIGGGR
                     INICQIDGITTNFGGDHNTLIVHNQDTPGHVATVSECLSAHGVNIATMQLYRSAAGGY
                     AVMVLECDEPIPDAVHAELACLPGIVKVTILNL"
     gene            422..1312
                     /gene="sdaAA"
                     /locus_tag="ACZPAX_02620"
     CDS             422..1312
                     /gene="sdaAA"
                     /locus_tag="ACZPAX_02620"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006574714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HIR1112823.1"
                     /translation="MAFSSVRDMVELCKESGKPLYEVILESDLAESGLTRAESEAEMH
                     RLWAVMRSTSDGYCGTDRSMSGFAGGDAAKVSAAAAQGALYADGFFADVMSEALKTAE
                     CNACMKRIVAAPTAGSCGVLPAVLLPLWRRGLADEDAVHHALYVAAGFGQVVAARATL
                     AGAEGGCQAEVGAASGMAAAALVDIKGGTAEQCAEAFAMALTNLEGLVCDPVAGLVEI
                     PCIKRNVIGAMNAVSAADMALAGVVGHIPADEVIDAMAEVGNAMSKDLRETGIGGLAG
                     TPTGRAICEIVTMNSGEEED"
     gene            1630..3033
                     /locus_tag="ACZPAX_02625"
     CDS             1630..3033
                     /locus_tag="ACZPAX_02625"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HIR1112824.1"
                     /translation="MNVNDPRWASIDAFVEGNRDNILRDITRLVAVPSVEGTPEPGAP
                     FGPGPKAALAKALEIADELGLDTFNADSYIGWAETGKIADGQKFLATITHTDVVPEGN
                     GWDADPYTVRVRDGWLLGRGVADDKGPSILCLYALKYLKDSGAALKYPVRALLGANEE
                     TNMHDVDYYAEHYPMPAFCFTPDAEFPVCNGEKGGFNGEIVSPKFEKGIITAFEGGVA
                     HNAVPDRASCTVQVGAGALIQTEGVTFEAGEGGSTVIRGWGKSGHAAMPEGTVNAISL
                     IVDCLLKSGVCTPQEEAYLQVLHTLHAATDGSALGIAADDGLFDPLTIIGGTIEMQDG
                     IIRQSFDCRYPTNTDPEKMTAAMEKVCGTAAKIENVMSRVPFYIDADSPAIQTLINTY
                     NDVTGEGKTPFTMGGGTYARHFPYAVSFGPEHTDIVIPDFAGPMHGANEGTPFEKLIE
                     ALKIYILALLRLQEIDL"
     gene            3334..4632
                     /locus_tag="ACZPAX_02630"
     CDS             3334..4632
                     /locus_tag="ACZPAX_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009321775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit C"
                     /protein_id="HIR1112825.1"
                     /translation="MPHTKATAGQPTNKMPVPEHVVIPMSMHIGAPAEPVVKKGDTVM
                     VGTMIGKAGGFVSANIYSSVSGTVQDVAPLRMANGSMATAVAIKTDGAQTVDPACVPP
                     VVTDKASLMAAVQACGLVGVGGAGFPTHVKLAANTIDTLLINAAECEPYLTTDCREMM
                     ECSDTIISGITAVMKYCEIPHCIIGIERNKPECIDLLTSLTREMKGVEVKGLPMRYPQ
                     GAEKTLVETCTGREVPQVGPSGKPGLPADAGCVIMNVTSVSTLGKFLKTGIPLVSKRV
                     TVEGDAIAKPQNIEVPIGTLYRDVIEACGGIKEGVELGKIIFGGPMMGAAAPSADFPV
                     LKQNNGLLLFSKQAAALPEPSACIRCGRCIEACPMGLEPVVIAQDFANKDFSALKARC
                     VDLCVACGSCTYACPAKRPVSQTMGLAKGWYMAELRKGGK"
     gene            4636..5592
                     /locus_tag="ACZPAX_02635"
     CDS             4636..5592
                     /locus_tag="ACZPAX_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015537318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HIR1112826.1"
                     /translation="MEDRLIVTASPHIREASTTRGLMGNVVIALLPAVLAAGLIFGVQ
                     AIVLVAVTTLACVAFEYLYEMLLKKPNTVGDLSAVVTGIILALNMPVGMPLWIAVVGA
                     FVAIVITKQLFGGLGYNFANPALVGRIVLFLGFTSRMTAYVYPDMAVDALASATPLAV
                     ADKTSLSLLDMFLGLHGGMMGEVCVLAILLGFAYLVATKTIQATIPVTIVATVFVLTA
                     LNTGSPYTALIECMSGGLLFGSVFMATDYVTSPFTTKGKLFYGVFIGLVTFLIRHFGS
                     MNEGMSYAILLGNLMTPWFNAWGHQTPLGYKKPKKAKKGGAE"
     gene            5592..6167
                     /locus_tag="ACZPAX_02640"
     CDS             5592..6167
                     /locus_tag="ACZPAX_02640"
                     /inference="COORDINATES: protein motif:HMM:NF016121.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HIR1112827.1"
                     /translation="MAEKQNTEKQSAFKELVLPVIVLVVICLVCSALLAVLNDITAPI
                     IEENTQAETLAAYVSVLPEGTTTDSMTAIDGLTTANVQGAVKTANGDVAVKAAASGYS
                     GKDVTVYVAFSADGTVSGISIDGSTQTTGIGSKVSGDSFASGFIGWNGGEVSSGSPVD
                     SIAGATYSSNGAFNAINAAIDCYNNEIKGVQ"
     gene            6170..6862
                     /gene="rsxE"
                     /locus_tag="ACZPAX_02645"
     CDS             6170..6862
                     /gene="rsxE"
                     /locus_tag="ACZPAX_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006875881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HIR1112828.1"
                     /translation="MNEKPSKWKVFTAGIIRENPVLRLVLGCCSALAVTTTVSGALGM
                     GLAMTFVLVCSNIVISALRKVIPAKVHLPCYIVIIATFVTVVQMVLQAFVPDLYKTLG
                     VYLALIVVNCIILGRAEMFACKNTVVDSALDGLGMGIGYILTMVLMSSIREIIGSGTW
                     MGITLIPESVDRMTVMNSAPGGFFVFGCLMAGCVWLERKLNKPIERKSCGDVMLEKID
                     AAKDEQKEGAAK"
     gene            6862..7503
                     /locus_tag="ACZPAX_02650"
     CDS             6862..7503
                     /locus_tag="ACZPAX_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex protein RnfA"
                     /protein_id="HIR1112829.1"
                     /translation="MSTHIATFATIFFSMILVNNYVLVQFLGICPFLGVSKKLDSSAG
                     MGAAVIVVMVIATAVTFPLQIFLLDAYNLGYLQTIIFILVIAVLVQLIEITLKKYVPA
                     LYQSLGVYLPLITTNCCVLGVTILAVQDYSAIVAEYGFGLAYAEALVCAAGAGVGFLV
                     AMLLFCGVRSRVEAANPPESFKGLPITLVSAAITSMSFMGFGGLVENIINAIF"
     gene            7522..8370
                     /locus_tag="ACZPAX_02655"
     CDS             7522..8370
                     /locus_tag="ACZPAX_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HIR1112830.1"
                     /translation="MNPIVFAIVVVVVLGLAGGVILVLASKFMAVYEDPRIAQITECL
                     AGANCGGCGYAGCADYAKAIVENGAPTFKCAPGGDKAADAINTIMGNATDDRPSLRAT
                     VICAGGENCGKRFDYQGIQTCAAAASIAGGPSACAYGCLGLGDCTRACKFDAIHVING
                     VAVVDRKKCTGCTACTVVCPRHVIQMKPIAPQPAVKCSNKDKGALVNKTCKVGCIACG
                     LCVRNCPNQAIFLKDNVAVIDYTKCNGCGTCVSKCPKKAIQWVEGVPRSIDAPVPEHE
                     VLKTRV"
     gene            complement(8559..9710)
                     /locus_tag="ACZPAX_02660"
     CDS             complement(8559..9710)
                     /locus_tag="ACZPAX_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005352085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HIR1112831.1"
                     /translation="MEITNDILYVGVNDHLVDLFEGQYDVPNGMAYNSYLICDDKVAV
                     MDSVDAHFTDEWITKIAAALGERTPDYFVVQHMEPDHSGSVAAFAQAYPGTTIVASAQ
                     AFNMMKAYFGTDYTDRRVVVKEGDTLPLGRHTLHFVTAPMVHWPEVIMTYDDADKVLF
                     SADAFGKFGALDVEEPWLPEARRYFIGIVGKYGVQVQAVLKKAAGLDIETVCSLHGPV
                     LHKEQLADVLAAYDTWSAYRPETEGVLVAYSSIYGHTAEAANRLAEALREKGVETIAM
                     DLARCDMAEAVAQAFRFSKLVLATPTYNADVFPFMKEFIHHLTERNYQNRTVAFIENG
                     SWAPMAAKVMTKMLEGSKNLTYAATTVTVRGALNAANEAQIAALADELS"
BASE COUNT         1855 a         3032 c         2962 g         1873 t
ORIGIN      
        1 gccagcggcc tgcactataa aatcggctcc atcaacctgc gcggcgccca tcccaacacg
       61 gcggtactgc ggctggtcgg cgtgtcaggc cgcaagctgg aggtcgtcgg cgcgtccatc
      121 ggcggcgggc gcatcaatat ctgccagatc gatggcatca ccaccaactt tggcggtgac
      181 cacaacaccc tcatcgtcca caaccaggat acccccggtc acgtggccac cgtgtccgag
      241 tgcctgagcg cccacggcgt caacatcgct accatgcagc tctaccgttc cgcagcgggc
      301 ggctacgccg tcatggtgct ggaatgcgac gagccgattc cggacgccgt ccacgccgag
      361 cttgcctgcc tgccgggcat cgtcaaagtc accatcctaa atctgtaagg gggcgggaaa
      421 gatggcattt tcttcagttc gcgacatggt cgagctctgc aaggagagcg gcaagccgct
      481 gtacgaggtc attctggaaa gcgacctggc cgagagcggt ctgacccgcg ccgagagcga
      541 ggccgagatg caccgcctgt gggcggtcat gcgcagcact agcgatggct actgcgggac
      601 cgaccgctcg atgagcggct ttgcgggcgg cgatgcggcc aaagtcagcg cagcggcggc
      661 ccaaggtgcg ctgtatgccg acggcttttt tgccgatgtg atgtccgaag cgctgaaaac
      721 cgccgagtgc aacgcctgca tgaagcgcat cgtagcggcc cccacggcgg gcagctgcgg
      781 cgttttgcct gcggttttgc tgccgttatg gcggcgcggg ctggcagatg aggacgccgt
      841 acatcacgct ttgtatgtgg ccgctgggtt cgggcaggtc gtggccgcgc gggccacgct
      901 ggccggagcc gaaggcggct gccaagccga ggtgggtgcc gccagcggca tggcggcggc
      961 ggctctcgtg gacatcaagg gcggcacagc cgagcagtgc gccgaggcct ttgccatggc
     1021 gcttacgaat ttagagggcc ttgtctgtga cccggtggcc gggctggtgg agattccctg
     1081 catcaagcgc aacgttatcg gcgcaatgaa cgcggtatcc gccgccgata tggcgctggc
     1141 gggcgtcgtg gggcacatcc ccgccgacga ggtcattgac gcgatggccg aggtcggcaa
     1201 cgccatgagc aaagatttgc gcgagaccgg cattggcggt ctcgcaggca cccccacagg
     1261 ccgtgcgatt tgtgagatcg tgacgatgaa cagcggggaa gaggaagatt gacggcaaac
     1321 tgcggcgagg ctgcgcggct gggtgagggc accccgccct acgccaaacg ccatcacaaa
     1381 ccacagggcg gcatggcccc atgccgccga cgataacccg atgatgctta tgagtattgc
     1441 cctgcggccg ctctgcacac caccattccc gctggaacgc attgaggaaa tgaccgcgct
     1501 gatgggcgga gaaggaagga tgcggtgaat gtttgcgggg cggcaaatac catcctcccg
     1561 atccaacttt ctgtttacaa attccgccaa tgtggtatac tggaagaaaa aaatcaggag
     1621 gaaactctta tgaacgtgaa cgatccgcgc tgggcgtcga ttgacgcctt tgtggaaggc
     1681 aaccgcgaca acatcctgcg tgacatcacc cgcctggtgg cagtccccag tgtcgagggc
     1741 acgcccgagc cgggcgcacc attcggcccc ggccccaagg ccgcgctggc caaggcgctg
     1801 gaaatcgccg atgaactggg cctggacacc ttcaacgccg acagctacat cggctgggcc
     1861 gagaccggca agattgccga cggccagaag ttccttgcca ccatcaccca caccgacgtt
     1921 gtgcccgagg gcaatggctg ggacgctgac ccctacaccg tccgcgtgcg ggatggctgg
     1981 ttgttgggcc gcggcgtggc cgacgataaa ggccccagca tcctgtgcct gtatgcgctg
     2041 aaatacctga aagacagcgg tgcagcgctg aaatacccgg tgcgtgcgct gctgggtgcc
     2101 aacgaagaga cgaatatgca cgacgtagac tactacgccg agcattaccc gatgcccgca
     2161 ttctgcttca cccccgacgc cgagttcccg gtctgcaacg gcgaaaaggg cggctttaac
     2221 ggcgagatcg tcagccccaa gtttgagaag ggcatcatca ccgcctttga gggcggcgtg
     2281 gcccacaacg ccgtgcctga ccgcgcatcc tgcaccgtgc aggtgggcgc tggcgcgctg
     2341 atccagaccg aaggcgtgac ctttgaggca ggggagggtg gttccaccgt catccgcggc
     2401 tggggcaaga gcggccacgc tgccatgccc gagggcaccg tcaacgccat cagcctgatc
     2461 gtggactgcc tgctcaagag cggtgtctgc accccgcagg aggaagccta cctgcaggtg
     2521 ctgcacaccc tgcacgccgc caccgatggc agcgccctgg gcattgccgc agacgacggc
     2581 ctgtttgacc cgcttaccat catcggcggc acgattgaga tgcaggacgg catcatccgc
     2641 cagagcttcg actgccgtta ccccacgaac accgaccctg aaaagatgac cgccgccatg
     2701 gagaaagtct gcggcacagc ggcaaaaatc gagaatgtga tgagccgtgt gccgttctac
     2761 atcgacgcgg acagcccggc catccaaacg ctcatcaaca cctacaacga tgtgaccggc
     2821 gagggcaaga cccccttcac gatgggcggc ggcacctacg cgcgccactt cccgtatgca
     2881 gtcagcttcg gccccgagca caccgacatc gtcatcccgg attttgccgg cccgatgcac
     2941 ggtgccaacg agggcacgcc gtttgaaaag ctgatcgagg cgctgaagat ttatattctg
     3001 gcgctgctgc ggctgcagga gattgatctg taaataagaa atgggcactt gtttcgacag
     3061 gtgtccattt tctttgttgg attgttaatt tttatacacc tttcttatcc tgcatcgatt
     3121 gcgaaaattg ttgcaaatct tgcgggttcg gggcaaaata gcctgtttat cccatttgat
     3181 tgcttgactt gaaccccaaa gatgatacaa ttaaagtgta ttgttatccg cctgtgcagg
     3241 ggagcaaacc tgacctgccg gacgagaaaa atgagagaaa cgggggaaat cagattgttc
     3301 aagtccctaa agcgctctgc caagggtgct gctgtgccgc atacgaaggc gacagccggg
     3361 cagccgacca acaagatgcc ggtgcctgag cacgttgtca ttcccatgtc catgcacatc
     3421 ggcgcgcccg cagagccggt cgtcaaaaaa ggcgacaccg tcatggtagg caccatgatc
     3481 ggcaaggccg gcggctttgt gtccgccaac atctattcca gcgtgtccgg caccgtgcag
     3541 gatgtcgccc cgctgcgtat ggccaacggc agcatggcta ccgccgttgc catcaagacc
     3601 gatggtgcac agaccgttga cccggcctgc gtgccgccgg tcgtcaccga caaggcaagc
     3661 ctgatggcag ctgtccaggc ctgcggtctg gtgggcgtcg gcggcgcggg cttcccgacc
     3721 cacgtcaagc tggcggccaa caccatcgac acgctgctca tcaacgcggc cgagtgtgag
     3781 ccgtacctga ccaccgactg ccgcgagatg atggagtgca gcgacacgat catctccggc
     3841 atcaccgctg tcatgaagta ctgcgagatc ccgcactgca tcatcggcat cgagcgcaac
     3901 aagcccgagt gtatcgacct gctcacctcg ctgacccgcg agatgaaggg cgtcgaggtc
     3961 aagggcctgc ccatgcgcta cccgcagggc gccgagaaaa cgctcgtcga gacctgcacc
     4021 ggccgcgaag tgccgcaggt cggcccttcc ggcaagccgg gcctgcccgc ggatgccggc
     4081 tgcgtcatca tgaacgtgac ctctgtgtcc acgctgggca agttcctcaa gaccggcatt
     4141 cctctggtgt cgaagcgcgt caccgtcgag ggcgatgcca tcgccaagcc ccagaacatc
     4201 gaggtgccca tcggcacgct gtaccgcgac gtgatcgaag cctgcggcgg catcaaagag
     4261 ggtgtcgagc tgggcaagat catcttcggc ggcccgatga tgggtgccgc cgcccccagc
     4321 gcagatttcc cggtgctcaa gcaaaacaac ggcctgctgc tgttcagcaa gcaggctgcc
     4381 gccctgcctg agccttcggc ctgcatccgc tgcggccgct gcatcgaagc ctgccccatg
     4441 ggcctggagc ctgtggtcat tgcgcaggac ttcgccaaca aggacttctc tgcgctgaaa
     4501 gcacgttgcg tcgatctgtg cgtagcctgc ggcagctgca cctacgcctg cccggccaag
     4561 cgccccgtca gccagacgat gggccttgcc aagggctggt acatggccga gctgcggaaa
     4621 ggaggcaaat aacccatgga agatcgtctg atcgttactg cctcgccgca tatccgcgaa
     4681 gcatccacca cccgcgggct gatgggcaat gttgtcattg cgctgctgcc cgccgtgctg
     4741 gctgccgggc tgatcttcgg cgtgcaggcc attgtgctgg tcgctgtgac cacgctggcc
     4801 tgtgtggcgt ttgaatacct gtatgaaatg ctactgaaaa agccgaacac ggtgggggac
     4861 ctgtccgccg ttgtcaccgg catcatcctg gcgctgaata tgcctgtcgg tatgccgctg
     4921 tggatcgccg tggtcggtgc gtttgtggct atcgtcatca ccaagcagct cttcggcggc
     4981 ctgggctaca actttgccaa ccccgccctc gtaggccgta tcgtgctgtt cctgggcttc
     5041 acctcccgca tgaccgctta cgtctacccc gacatggccg ttgacgcgct ggcctccgcc
     5101 acgccgctgg ccgtggcgga caagacgagc ctgtccctgc tggatatgtt cctgggtctg
     5161 cacggcggca tgatgggtga ggtctgcgta ctggcaattc tgcttggctt tgcctatctc
     5221 gttgccacta agaccatcca ggccacgatc cctgtcacca tcgtggcgac cgtgttcgtg
     5281 ctgacggccc tcaacaccgg cagcccctac actgccctca tcgagtgcat gtccggcggt
     5341 ttgctgttcg gctctgtctt tatggcgacc gactacgtca ccagcccctt caccaccaaa
     5401 ggcaagctgt tctacggcgt gttcatcggc cttgtcacct tcctcatccg ccatttcggc
     5461 tcgatgaatg agggcatgag ctacgccatc ctgctgggca acctgatgac cccctggttc
     5521 aacgcctggg gccaccagac gccgctgggc tacaaaaagc ccaaaaaggc caagaaaggc
     5581 ggtgctgaat aatggctgaa aagcagaata ccgagaagca gagcgccttc aaggagcttg
     5641 tgctgcctgt catcgtgctg gtcgtcatct gcctggtctg cagcgccctg ttggccgtgc
     5701 tgaacgacat caccgcaccg atcattgaag aaaacaccca ggccgagacc ctggccgcct
     5761 atgtctccgt tttgcccgag ggcacgacca ccgacagcat gactgccatc gacggcctga
     5821 ccaccgccaa tgtgcagggt gccgtcaaga ccgccaacgg cgatgtggcc gtcaaggctg
     5881 cggcctccgg ctactccggc aaggacgtca ccgtctatgt cgccttcagc gccgacggca
     5941 ctgtctccgg catttccatc gatggctcca cccagaccac cggcatcggc tccaaggttt
     6001 ctggcgactc ctttgcctcc ggcttcatcg gctggaatgg gggagaggtc agcagcggca
     6061 gccctgtgga tagcattgcc ggtgcgacct actccagcaa tggcgcgttc aacgccatca
     6121 atgcagccat cgactgctac aataacgaga tcaagggggt gcagtaagga tgaacgaaaa
     6181 accgagcaag tggaaagttt ttacggccgg tatcatccgt gaaaaccctg ttctgcgcct
     6241 ggtgctgggc tgctgctcgg cactggctgt caccaccacc gtttccggtg cgttgggcat
     6301 gggcctggcc atgacgtttg ttctggtctg ctccaacatc gttatctcag cactgcgcaa
     6361 ggtcatcccg gccaaggtgc acctgccgtg ctacatcgtc atcattgcaa catttgttac
     6421 cgtcgtacag atggttttgc aggcattcgt gcccgacctg tacaagacgc tgggcgtcta
     6481 cctggcgctg attgtcgtca actgcatcat cctcggccgt gccgagatgt tcgcctgcaa
     6541 aaacaccgtc gtggactctg cgctggacgg cctgggcatg ggcatcggct acatcctgac
     6601 catggtgctg atgtcctcca tccgcgagat catcggcagc ggcacctgga tgggcattac
     6661 cctcatcccc gagagcgtgg accgcatgac cgtcatgaac tctgcgccgg gcggcttctt
     6721 cgtctttggc tgcctgatgg ccggctgcgt ctggctggaa cgcaagctga acaagcccat
     6781 cgagcgcaag agctgcggcg acgtgatgct ggaaaagatc gacgccgcta aagatgagca
     6841 gaaggagggt gccgcaaaat gatgagtaca catatcgcaa cgtttgccac catcttcttc
     6901 agcatgattc tggtaaataa ctacgttctg gtgcagtttt tgggcatctg cccgttcctc
     6961 ggcgtttcca aaaagctgga ttcctccgcg ggcatgggcg ctgccgttat cgtcgtcatg
     7021 gtcattgcca cggccgtgac cttcccgttg cagatcttcc tgctggatgc ctataacttg
     7081 ggctatctgc agaccatcat cttcatcctc gtcattgccg tcctcgtgca gttgatcgag
     7141 atcacgctga aaaagtacgt ccccgcgctg tatcagagcc tgggcgtcta tctgccgttg
     7201 atcaccacca actgctgcgt gctgggcgtc accatcctgg ccgtgcagga ctactccgct
     7261 atcgtggccg agtacggctt cggcctggct tacgccgagg cgcttgtctg cgccgccggt
     7321 gcgggtgtcg gcttcctggt cgccatgctg ctgttctgtg gcgtgcgttc ccgcgtcgag
     7381 gctgccaacc cgccggagag cttcaagggc ctgcccatca cgctggtttc cgccgctatc
     7441 acctccatga gcttcatggg ctttggtggc ctggtcgaaa acatcatcaa cgcgatattc
     7501 taagaaaggg gaatggagag aatgaacccg atcgtatttg ctatcgttgt cgtggtggtg
     7561 ctgggcctgg ccggcggcgt gatccttgtg ctggcttcca aattcatggc tgtgtatgag
     7621 gacccgcgca tcgcgcagat caccgaatgt ctggcaggcg ccaactgcgg cggctgcggc
     7681 tatgccggct gtgccgacta cgccaaggcc atcgttgaga acggtgcccc caccttcaag
     7741 tgcgcaccgg gcggcgataa ggctgccgac gccatcaaca ccatcatggg caatgccacc
     7801 gatgaccgcc ccagcctgcg cgccaccgtt atttgtgcgg gcggcgagaa ctgcggcaag
     7861 cggtttgatt accagggcat ccagacctgc gccgccgcgg cttccatcgc gggcggcccc
     7921 agcgcttgtg cctatggctg cctgggcctg ggcgactgca cccgcgcctg caagtttgac
     7981 gccatccacg ttatcaacgg cgtggccgtc gttgaccgca aaaagtgtac cggctgcacg
     8041 gcctgcactg tggtctgccc gcgccacgtc atccagatga agcctattgc cccgcagcct
     8101 gcggtcaagt gctccaacaa ggacaagggc gcactggtca acaagacctg caaggtcggc
     8161 tgcattgcct gcggcctgtg cgtgcgcaac tgcccgaatc aggccatctt cctgaaggat
     8221 aatgtcgctg tcatcgacta caccaagtgc aacggctgcg gcacctgcgt ttccaagtgc
     8281 ccgaagaagg ccatccagtg ggtcgagggt gtcccgcgct ctatcgacgc ccccgtgccc
     8341 gagcatgagg tgctcaagac ccgcgtgtaa ttcgcagtaa gaccacgcaa aaaaacaagc
     8401 ccgccccgaa ccgcgagaaa gcggcccggg gcgggctttg ctgttaggaa tattattagg
     8461 gcaataccgc ataattctaa gtgccgatac ggcgagcgag gtgcggcagc cgccggagcg
     8521 gtgcttaagc tgtcaggcgg gaattgtgcg gtattgcctt agctcagttc gtcggccagg
     8581 gcggcgatct gggcctcgtt ggcggcattc agtgcgccgc gcacggtgac ggtggtggcg
     8641 gcgtaggtca ggttcttgct gccctccagc atcttggtca tgaccttggc ggccatcggt
     8701 gcccaggagc cgttctcaat aaacgcgacc gtgcggttct ggtagttgcg ctcggtcagg
     8761 tggtggatga attccttcat gaaggggaac acgtcggcgt tgtaggtcgg cgtagccagc
     8821 accagcttgc tgaagcggaa cgcctgggcc acggcctcgg ccatatcgca gcgggccaga
     8881 tccatggcga tcgtctcgac acccttttcc cgcagggcct cggccaggcg gttggcagcc
     8941 tcggccgtgt gaccatagat gctgctgtac gccaccagta cgccctcggt ctcggggcgg
     9001 taggcgctcc atgtgtcgta ggcggccagc acatcggcca actgctcttt gtgcagcaca
     9061 gggccgtgca ggctgcacac ggtctcgata tccagcccgg cggccttttt cagcacggcc
     9121 tgcacctgca caccgtactt gcccacaatg ccgatgaagt agcggcgggc ctcgggcagc
     9181 cacggctcct caacgtccag tgcgccgaat ttgccgaagg catcggcgct gaacagcacc
     9241 ttgtccgcat cgtcataggt catgatgacc tcgggccagt ggaccatcgg cgcggtgaca
     9301 aaatgcaggg tatggcggcc cagcggcagg gtgtcaccct ccttgacgac aacgcggcgg
     9361 tcggtgtagt cggtgccgaa gtaggccttc atcatgttga aggcctgtgc ggaagcaacg
     9421 atggtcgtgc cggggtaggc ctgcgcgaac gcggccaccg aaccggagtg atccggctcc
     9481 atgtgctgca caacaaagta atcgggcgtg cgctcgccca gcgcggcggc gatcttggtg
     9541 atccattcgt cggtgaagtg ggcatcgacg gaatccatga cggcaacctt gtcgtcgcag
     9601 atcagatagg agttgtaggc cataccgttg ggcacgtcgt actggccctc aaacaggtca
     9661 acaaggtggt cgttgacgcc gacatataag atatcgttgg tgatttccat agttgcaaac
     9721 ct
//
LOCUS       DBMDSQ010000040         9491 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_2031_length_9491_cov_1.480192, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000040 DBMDSQ010000000
VERSION     DBMDSQ010000040.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 9491)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9491)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9491
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2031_length_9491_cov_1.480192"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1914)
                     /gene="recD2"
                     /locus_tag="ACZPAX_02665"
     CDS             complement(<1..1914)
                     /gene="recD2"
                     /locus_tag="ACZPAX_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0043139 - 5'-3' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SF1B family DNA helicase RecD2"
                     /protein_id="HIR1112832.1"
                     /translation="MAEKQNQQLMKLEGVVEHVIYENAESGYAVFEVDAGGTDVVVAG
                     NVGGVDNGMSVTVYGHMVNHPSYGEQFKAETIEASLPEDTSAILSYLSSGVLPYIGPS
                     TAKKLVAKFGEDTLNVIAQTPQKLCELKGINEQKAAIISNEFRRMYGVREVVAWFARY
                     GLTAQQAVTAYRAFGPHTVEALTQNPYLLCGEPLQLKFGQVDGIAAALQFETGCRLRV
                     AAGLLYALRHNAGNGHTCLPRDKLLDSTSKFLRVPPDEIEAGLDELLSAEELRTRTFD
                     GVEYIYLPDLLSAEEDIAARLGQLSTFPTEVPKTLDADIRVLEMAQGFEYAPLQKQAI
                     KLALSSRVMVLTGGPGTGKTTTVNAILSLYEALYDRVALCAPTGRAAKRLSELTGHAA
                     STIHRLLEVDYSSGNVRFIHNEKNLLKYDVIILDEMSMVDVKLFQALLAAARYHCRII
                     MVGDADQLPSVGPGNILGEILRVGVVPTVRLTDIFRQAQQSLIVQNAHRIVEGRMPKK
                     GGAKDDFFLIESTGLACQKLVCDLVSKRLPKAYGFDPVKDIQVLCPTKVGPTGSVELN
                     KKLQEILNPPAKGRAQIGTAESAKILRLGDKVMQVKNDCDITFERDGAEAGVGAYNGD
                     LGIITAVDVDARAV"
     gene            complement(2050..3198)
                     /gene="metK"
                     /locus_tag="ACZPAX_02670"
     CDS             complement(2050..3198)
                     /gene="metK"
                     /locus_tag="ACZPAX_02670"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016146694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HIR1112833.1"
                     /translation="MSKVLFTSESVTEGHPDKICDQISDAILDAILAQDPGARVACET
                     TCSTGLVHIMGEITTSCYVDIPGTAREVIRSIGYDRAKYGFDCDTCGVITNIDGQSPD
                     IALGTNDEVAGAGDQGMMFGYACNETPELMPLPISLAHKLAKRLTEVRKNGTLDYLRP
                     DGKSQVTVEYNDGKPVRIDAVVISSQHSPDVSQEQLHEDIRREVIEKVIPAELLDENT
                     KYYINPTGRFVVGGPQGDSGLTGRKIIVDTYGGYARHGGGAFSGKDPSKVDRSAAYAA
                     RWVAKNVVAAGLATKCEVELAYAIGVAKPVSIMVDTFGTGTVADEKIEQAVEKVFDLT
                     PAAIIRDLDLRKPIYRQLAAYGHMGREDLGVKWESTDRADALKAAVAE"
     regulatory      complement(3284..3382)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(3502..4098)
                     /locus_tag="ACZPAX_02675"
     CDS             complement(3502..4098)
                     /locus_tag="ACZPAX_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005941988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032217 - riboflavin transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0032218 - riboflavin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HIR1112834.1"
                     /translation="MQNSTTNVRKLTGTAMLGAVAAVLMYLEFPIPIMPSFVKLDVSE
                     LPALLAAYAYGPLSGIAVCLIKNLIKLPSTSTAAVGELFNFVMGALFVGVAGFVYKRS
                     KTRRSALIGAGMGALAMAVVSVPYNYFIVYPAYVVMYHLPLEAIIGMYQAINPSVDGL
                     LACLLTFNLPFTLFKGLLDAVLCFLIYKPLSPILHGRK"
     regulatory      complement(4228..4366)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            4452..5504
                     /locus_tag="ACZPAX_02680"
     CDS             4452..5504
                     /locus_tag="ACZPAX_02680"
                     /inference="COORDINATES: protein motif:HMM:NF044972.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HIR1112835.1"
                     /translation="MFDFSFDLGTLVPSLVLALLAVVLPMLFCRIADGFLRRTKSHMA
                     SWLHILLESYVFPLAMILRVFLFLLAISMLPFVFVTDAKFQNVLDVGSDLLVIFFLGL
                     GSWRAAPVSRLLLHSAENRLDLQTHQTMGHFFENIFHALVVVFTGIAALDRLGVPVSG
                     LLTGAGVAGLAVSLAAQSTLSNLIAGITLVLEHPFGIGDYVVFGDYEGTVEDISFRST
                     RIRTPDNVVITIENSKVAAEYIQNCDCRTSRLWSFTIGLTYDTPREKLERLTTDLTGL
                     LLAQPDVKENGVSVTLDKFNDYSIDLLCRVYITKTSLRDYLQVKNRLNLLILDKVRAD
                     DCEFAFPTTTLDMPKS"
     gene            complement(5561..6043)
                     /locus_tag="ACZPAX_02685"
     CDS             complement(5561..6043)
                     /locus_tag="ACZPAX_02685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1112836.1"
                     /translation="MKCFSAAATVVLAAMLCGCQQKADMTPVWQTDLPTTGVHMELSN
                     TDDISFDPAYYAQTREDAFYNNLHEISAVMAEGAPVVTFYGVTADEVKLIYLENIGDY
                     LYVDYDRVAPQEKLDYVRIDGDGYVSYKLDTAYNFEFTVTTDKGEDQYLVVSRRNDIQ
                     "
     gene            complement(6053..6574)
                     /locus_tag="ACZPAX_02690"
     CDS             complement(6053..6574)
                     /locus_tag="ACZPAX_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017753321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1112837.1"
                     /translation="MRETSYHAVIGEMTVRALWEMQNVMDCIPDELWDHCYGGAPLWQ
                     HLYHTLHHLDQWFINPRDNDFVEPPVHTPHLQELDIYPATHLSRRDMDGYFYTIKAKL
                     SIYLTSLHDEDLLQRPENCEWTRFTLILSQYRHLYMHMGMLMGFVEAETGLCPRILKV
                     GEELPTPPYDPYE"
     gene            complement(6620..7852)
                     /locus_tag="ACZPAX_02695"
     CDS             complement(6620..7852)
                     /locus_tag="ACZPAX_02695"
                     /inference="COORDINATES: protein motif:HMM:NF016032.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - modified amino acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-cysteine ligase family protein"
                     /protein_id="HIR1112838.1"
                     /translation="MAKLDEQIKTMAGYFTADCEADGRLSLVLEVEHFITCSDGRAAD
                     FAAVQAAMQDMQQQADAPIIVDGTYMGYSGLAASVTVGPACQLCLTLAPQRDVQDMMD
                     LYNRLYLQMGLALAAHGLRAWALGCHPTTRAEALPLVPRTRDEAMDRYFKNTGSCGTQ
                     VMRGTAATLLSIDYFDEMDFVRKMRVASLLTPFFALLCDNAPIYQGSRNSMCSVRTRI
                     WQDVDHDRCGVVPHLMDPDFGFARYAENVLTKPQITAWRTGRVKAVGSKTAPELYAAH
                     LSQREAAQILSNFFYDVRLKSRIELRAADSMPPRYIAAYAQLVKSVFGSPAALQNVLR
                     HYAGITTLDITTAKLAVCKDGFNALAYGKPISSELTWLLMQARSRTPSQEERALLDPF
                     MALVMKRKTIRETENYNE"
     gene            complement(8058..9023)
                     /locus_tag="ACZPAX_02700"
     CDS             complement(8058..9023)
                     /locus_tag="ACZPAX_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015552862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HIR1112839.1"
                     /translation="MKEISITSFDNLQIGQAENAAAGTGCTVFLLGKDGAPAGLDVRG
                     GGPASRESELLKPPAAAQVIHAIVLAGGSAFGLDAAGGVMRYLEERNIGYDVGVTKVP
                     LVCQADLFDLTVADARTRPDVAMAYQACANAENGGNYCDGNYGAGTGATVGKLLGMDH
                     CMKSGIGSYAVQVGPLKVGAVVAVNAAGDIYDYENGCKVAGLRADDGKTFLDSEQLML
                     EKIDVQENKFVGNTTIGVVFTNARFDKTRLCKIAGMAHDGYARTIRPVHTSADGDSIY
                     AVSLGDVAADQDAVGALAARVMAKAILRGVQAAEGAYGFPAARDL"
BASE COUNT         1897 a         2733 c         2700 g         2161 t
ORIGIN      
        1 cacggcgcgg gcatccacat ccacagcggt gatgatgcct aggtcgccat tgtacgcgcc
       61 gacgccagcc tcggcaccgt cgcgctcaaa ggtgatgtcg cagtcgtttt tcacctgcat
      121 aactttgtcg cccagacgca gaatttttgc actttcggcg gtgccgatct gggcgcggcc
      181 cttggccggc gggttcaaaa tttcctgcaa tttcttgttc agctccacgc tgccggtagg
      241 gccgactttg gtcgggcaaa gcacctgaat gtccttgacc gggtcaaaac cgtaggcctt
      301 gggcaggcgc ttactgacca ggtcgcagac gagcttttgg caggcaaggc cggtggattc
      361 gatcaagaag aagtcatctt tggccccacc cttcttgggc atccggccct cgacaatgcg
      421 gtgggcgttc tggacaatga ggctttgctg cgcctggcgg aaaatatccg tcaagcgcac
      481 cgtaggcaca acgcccacgc gcaaaatctc gcccagaatg ttgcccgggc cgacgctggg
      541 gagctggtcg gcgtctccta ccataataat gcggcagtga taccgcgccg ccgccagcag
      601 cgcctggaac agcttgacgt ccaccatgct catctcatcc aggatgatga catcatattt
      661 taaaaggttt ttttcgttat ggataaaacg cacattgccg ctgctgtaat ccacttccag
      721 caggcggtgg atggtcgaag ctgcgtgccc ggtcagttcg ctcaaccgct tggctgcgcg
      781 gcctgtgggg gcgcagagtg ccacacggtc atacagggcc tcgtacaggc tcagaatcgc
      841 gttgaccgtc gtcgtcttgc cggtgccggg gccgccggtc agcaccatca cgcggctgga
      901 cagtgccagc ttgatggcct gcttttgcaa cggcgcgtac tcaaagccct gggccatttc
      961 cagcacgcgg atatcggcgt ccagcgtctt gggcacctcg gtcgggaagg tggacagctg
     1021 ccccagccgc gcggcgatat cttcctcggc actgagcagg tcgggcaggt agatgtactc
     1081 caccccgtca aacgtgcggg tgcgcagttc ctcggcactg agcagttcat ccaggcccgc
     1141 ttcgatttcg tcgggcggca cgcgcagaaa ctttgacgtg gaatcgagca gtttatccct
     1201 tggcagacag gtgtggccgt tgcccgcatt gtggcgcagc gcgtagagca gtcccgccgc
     1261 gacacgcagg cggcagccgg tttcaaattg cagcgcggcg gcaatgccgt ccacctggcc
     1321 gaattttaat tgcagcggct ctccacagag cagatagggg ttctgggtca atgcctcgac
     1381 ggtgtgcggg ccaaaagcgc ggtaggcggt cacggcctgc tgggctgtca ggccatagcg
     1441 ggcaaaccag gccacgacct cccgcacgcc gtacatccgg cggaactcgt tggagatgat
     1501 ggcggctttc tgctcattga tgcccttgag ctcacagagt ttttgcgggg tctgggcgat
     1561 aacgttgagc gtatcttcgc cgaattttgc caccagcttt ttggcggtgg aaggcccgat
     1621 gtagggcagc acgccgctgg acaagtagct taaaatggcg ctggtatcct ctggcaggct
     1681 ggcctcgata gtctcggcct tgaactgttc gccatagctg gggtgattga ccatatggcc
     1741 gtagacggtc acggacatac cgttatccac gccgcccacg ttgcccgcaa caacgacgtc
     1801 ggtgccgcct gcatcgacct cgaacacggc atagccggac tcggcgtttt catagatgac
     1861 gtgctcgacg acgccttcca gcttcatcag ctgctggttc tgtttttcag ccatggtctg
     1921 ccccctttcc gccaaaatcg ccgcactttc acagataaag atattatacc ccatatgggg
     1981 gcgtttggca aatccccaaa acacaaaaaa tgtccctgca ccgttatggc gcagggacaa
     2041 aaatattttt tattcagcga cagcagcctt caaggcgtcg gcgcggtcgg tgctctccca
     2101 cttcacgccc aagtcctcac ggcccatgtg accgtaggca gccagctgcc ggtagatggg
     2161 cttgcgcagg tcaaggtcgc ggatgatggc agcgggggtc aggtcaaaga ccttctcgac
     2221 agcctgctca atcttctcat cagccaccgt gccggtgccg aaggtatcga ccatgatgga
     2281 aaccggcttg gcaacgccga tggcataggc cagctcgacc tcacacttgg tagccagacc
     2341 tgcggcaacc acattcttgg cgacccagcg ggcagcgtaa gcggcggaac ggtcgacctt
     2401 gctgggatcc ttgccgctga aagcaccgcc gccgtggcga gcatagccgc cgtaggtgtc
     2461 aacgataatc ttgcggccgg tcaggccgga gtcgccctgc gggccgccca caacaaagcg
     2521 gccggtgggg ttgatgtaat acttggtgtt ctcgtccagc agctcggcgg ggatgacctt
     2581 ctcgatgacc tcgcggcgga tgtcctcatg cagctgctcc tggctgacgt cggggctgtg
     2641 ctgactggaa atgaccacgg cgtcgatgcg gaccggcttg ccgtcgttat actcgaccgt
     2701 gacctggctc ttgccgtccg ggcgcagata atccagggtg ccgttcttgc ggacctctgt
     2761 caggcgcttg gccagcttgt gggccaggct gatgggcagc ggcatcagtt cgggcgtctc
     2821 gttgcaggcg tagccgaaca tcatgccctg gtcgcccgca ccggcaacct catcgttggt
     2881 gccaagggca atgtccgggc tctggccgtc gatgttggtg ataacgccgc aggtatcgca
     2941 gtcgaagccg tacttggcac ggtcgtagcc gatggagcgg atgacctcgc gggcggtgcc
     3001 ggggatgtca acgtagcagc tcgtggtgat ctcgcccatg atgtggacaa ggccggtgga
     3061 gcaggtcgtc tcgcacgcta cacgcgcacc gggatcctgg gccaggatgg catcgaggat
     3121 ggcatcggaa atctggtcgc agattttgtc gggatgcccc tcggtgacag attcggacgt
     3181 gaaaagaact ttggacatgg gaaaaagcct cctataaaat attcggcaga aacgcaaccc
     3241 tggaaacaaa aaagcgcaca gccggaaaga ctgtgcgcgt aaacgcttat ctttcggttt
     3301 cccgcaggac ttggcacctt gcgtttgcac gcaggttgtc gggcttcatc gggcctgttc
     3361 cctcagccgc tcttgataag ttgctattct gttgttgtgt agttagtgta ccatagactt
     3421 taggcaaagt caacgataat tggttgtgca gttcggcagg tttgcacaaa agtggggcag
     3481 attgcgtggc gtgtgtggtt tttactttct gccatgcaaa atcggcgaca ggggcttata
     3541 aataaggaag cagagcaccg catccagcag gcccttaaaa agggtgaacg gcagattgaa
     3601 ggtcagcagg caggccagca ggccatccac gctgggattg atagcctggt acatcccgat
     3661 gatggcctcc agcggcaggt ggtacataac cacataggcc gggtaaacga tgaaatagtt
     3721 gtacggtacg ctgacgacgg ccatagccag tgcgcccatc cctgcgccga tgagggcact
     3781 tcggcgggtc ttgctgcgct tgtagacaaa gcccgccacg ccgacgaaca gcgcacccat
     3841 gacgaagttg aacagctcac cgacagcggc cgtgctggtg ctgggcagct tgatcaggtt
     3901 cttgatcagg cagaccgcga tgccggacag cgggccgtag gcataggccg ccaacagggc
     3961 gggcagttcg gatacatcca gcttgacgaa ggacggcatg atgggaatgg gaaattccag
     4021 gtacatcaat acggcggcga cagcacccag catggcggta ccggtaagtt tgcggacatt
     4081 ggttgtagag ttttgcatga gatttacctc ccgaaaatag tgtgaaagtc agctcacgaa
     4141 cccgggaggc tctgccctgc cctttgcaaa agaaaacgcc ctgccgtaca aatatacagc
     4201 agggcgcaga aaagcaaagc agtcagcttt ccgtttcttt catccggact ataccgtcgg
     4261 ccccggaatc gcaccgggtc agcggctgcc aaaacagccg gtcgcgggct tacggcttgc
     4321 gccgtttacc gccggtgggg aatcacaccc cgccctgaaa cagactttct gtgcaatagt
     4381 atagcacagt ttttgcaaaa tgcaagaggg tgtggtataa tcagcgcaga aacttatgcg
     4441 cgaggtatcc tatgtttgat ttttcgtttg atttgggcac gctggttcct tctctggtgc
     4501 tggcgcttct ggccgttgtg ctgccgatgc tcttctgccg cattgccgat ggctttctaa
     4561 ggcgtaccaa gtcccacatg gcatcgtggc tgcacatttt gctggaaagt tacgtttttc
     4621 cgcttgctat gatactgcgt gtttttcttt tcttgctggc tatttccatg ctgccatttg
     4681 tatttgtcac agatgccaaa ttccagaatg tattggatgt cggctccgat ctgttggtca
     4741 tcttttttct tgggcttggc agctggcgcg ctgcgccggt ttcccgtttg ctgctgcaca
     4801 gtgcggagaa tcgtcttgac ttgcagaccc accaaacaat gggccacttt tttgagaata
     4861 tctttcacgc gctggtcgtg gtgttcaccg gcattgcggc gctggaccgc ctgggtgtgc
     4921 cggtcagcgg tctgctgacc ggtgccgggg ttgccggcct cgctgtttca ctggctgcgc
     4981 agtccacact gtccaacctg attgccggca tcacgcttgt gctggagcat ccttttggta
     5041 ttggggatta tgtggttttc ggagactatg aaggaaccgt cgaggatatc tccttccgct
     5101 ccacacggat acgtaccccg gataatgttg ttattacgat agaaaactcg aaggttgccg
     5161 ccgagtatat tcaaaactgt gattgccgca caagccgcct gtggagtttt accatcggcc
     5221 ttacctatga taccccccgc gaaaagctgg aacgtctgac aacagacttg acaggtctgc
     5281 tgcttgccca gcctgatgtg aaggaaaacg gcgtaagtgt cacgcttgat aaattcaatg
     5341 attacagtat cgacctgctg tgccgcgtgt acataactaa aacttctctg cgcgattatt
     5401 tgcaggtcaa gaaccgcctg aacctgctga ttcttgataa agtccgcgca gacgactgcg
     5461 agtttgcttt ccccaccacg acgctggata tgccaaaatc ttaatgtata aaatacctgc
     5521 cgcaagtgac atcgttcact tcccctgcgg caggcatttt ttattgaata tcgtttctcc
     5581 ggctcacgac cagatattga tcctcaccct tatccgtcgt gaccgtaaac tcaaaattgt
     5641 acgcggtgtc cagcttgtag gagacatagc cgtcgccgtc gatgcgcacg taatccagct
     5701 tttcctgcgg tgccacgcgg tcgtagtcca catagaggta gtcgccgatg ttttcaagat
     5761 aaatcagctt cacctcgtcc gccgtcacgc cgtagaaggt gacgaccggt gcgccctccg
     5821 ccatgacagc ggatatctcg tgcaggttgt tgtaaaaggc atcctcccgc gtctgcgcgt
     5881 agtaggcagg gtcaaagctg atgtcatcgg tgttgctcag ttccatatgt acgccggtgg
     5941 tgggcaggtc agtctgccac accggggtca tatctgcttt ttgctggcag ccgcacagca
     6001 tagccgccag caccaccgtc gcagcggcag agaaacactt cataaaaacc tctcattcgt
     6061 atgggtcgta gggcggcgtc gggagttcct cacccacctt caaaatgcgc gggcagaggc
     6121 cggtctcggc ctcgacaaag cccatcagca ttcccatgtg catatataag tgacgatact
     6181 gcgataaaat caacgtaaac cgtgtccact cgcagttctc gggccgctgc aataaatctt
     6241 cgtcatgcag cgaagtcaga taaatcgaca gttttgcctt tatcgtgtaa aaatagccgt
     6301 ccatgtcccg gcgcgacaag tgcgtggccg ggtagatgtc caactcctgc aagtgcggcg
     6361 tgtggaccgg cggttctaca aaatcgttgt cacgcgggtt gatgaaccac tgatccaggt
     6421 ggtgcagcgt gtggtacaaa tgctgccaca gcggtgcgcc gccgtaacag tgatcccaca
     6481 gttcgtcggg gatgcagtcc atgacgttct gcatctccca cagtgctcgc acggtcatct
     6541 ccccgatgac cgcatgatac gatgtttccc gcacggcaca gccccctttc aggggctagt
     6601 atacccgcag catcgtcgat tattcgttat aattttccgt ttcacggatg gttttccgtt
     6661 tcatgaccag tgccatgaag ggatccagca gggcgcgttc ctcctgggag ggtgtgcggc
     6721 tgcgggcctg catcagcagc caagtcagct cactgctgat gggcttaccg taagccagcg
     6781 cgttgaagcc gtccttgcag accgccagct tggccgtggt gatatccaac gtcgtgatgc
     6841 ccgcgtagtg ccgcagcacg ttttgcagcg cggccgggct gccgaacacc gatttgacca
     6901 gttgggcgta ggccgcaata tagcgcggcg gcatactgtc ggcggcgcgg agttcaatgc
     6961 gggatttgag ccgcacatcg taaaagaaat tggacaggat ttgtgcggct tccctctggc
     7021 tgaggtgggc ggcgtacagc tccggggctg tcttactgcc cacagccttt accctgccgg
     7081 tgcgccaagc ggtaatttgc ggcttggtca gcacattctc cgcatagcgg gcaaagccga
     7141 agtccgggtc catcaggtgc ggcacaacgc cgcagcggtc gtggtccaca tcctgccaga
     7201 tacgcgtgcg cacactgcac atggaattgc ggctgccctg gtagatgggc gcattgtcgc
     7261 agagcagcgc aaagaatggt gtaagcaggc tggccacacg cattttgcgc acaaaatcca
     7321 tctcatcaaa atagtcaatc gagagcagtg tcgccgctgt accgcgcatc acctgcgttc
     7381 cacaggagcc tgtatttttg aaataccggt ccatggcttc atcccgggtg cgcggcacca
     7441 ggggcagggc ctcggcgcgg gtggtggggt ggcagcccag cgcccaggca cgcaggccat
     7501 gcgccgccag cgccagcccc atttgcagat acaggcggtt gtacaggtcc atcatgtcct
     7561 gcacgtcccg ctgcggggcc agcgtcaggc agagctggca cgcaggcccc accgtgaccg
     7621 acgcggccag gccgctgtac cccatgtagg tgccgtctac gatgattggc gcgtcggcct
     7681 gctgctgcat atcctgcatg gctgcctgca cggcagcaaa gtccgccgcg cggccatctg
     7741 aacaggtgat gaagtgctcc acttccagca caagcgaaag tctaccgtca gcctcgcaat
     7801 ccgccgtgaa gtagcccgcc atggtcttga tttgttcatc cagctttgcc ataaaagtga
     7861 tggttctccc ctgtcgatcc tcgtgttgga tttattatac cgcgaaatcg ggcggtgtgc
     7921 aatggctgcg ggagagcggg ttttctctat tccgtgtata tctaccatgt acggtggcca
     7981 gtctcctggc caccgttggt atcgccctgt tgtagaggct acgtcccaga cgccccgcct
     8041 tacgtcggta atttatctta tagatcccgc gctgccggga atccgtaggc tccctcagcc
     8101 gcctgcacgc cgcgcagaat tgctttagcc atcacccggg ccgccagcgc acccactgca
     8161 tcctggtcag ctgccacatc ccccagcgag actgcgtaga tgctgtcccc atctgccgag
     8221 gtatgcaccg ggcggatggt gcgggcgtaa ccgtcgtgcg ccatgcctgc aatcttgcac
     8281 agccgtgttt tgtcaaatct ggcgttggta aataccacgc caatcgttgt attgccgacg
     8341 aatttattct cctgtacgtc gattttttcc agcatcaact gttcggagtc taggaaagtc
     8401 ttgccatcgt ctgcccgcaa acctgcgacc ttgcagccgt tttcgtagtc ataaatgtcg
     8461 cctgccgcgt tcacggccac aaccgcaccg actttcagcg ggccgacctg cacggcataa
     8521 ctgccgatgc cggatttcat gcagtgatcc atgcccagca gcttgcccac agtcgcgccg
     8581 gtgcctgcac cgtagttgcc gtcgcaatag ttgccgccgt tctcggcatt ggcacaggct
     8641 tggtacgcca tggcgacatc gggccgggtg cgggcatccg ccacggtcaa atcgaagagg
     8701 tctgcctgac agacaagtgg cactttcgtc acacctacgt cataaccgat attgcgttct
     8761 tccagatagc gcattacgcc gcccgcggcg tccagtccga acgcgctgcc ccccgccagc
     8821 acaatggcgt ggatgacctg cgccgccgcc ggcggtttga gcagctcgct ctcccgcgaa
     8881 gcaggcccgc cgccgcgaac atccagtccc gccggggcac cgtctttgcc cagcaggaac
     8941 accgtgcacc cggtccccgc tgctgcgttc tctgcctggc cgatctggag attgtcaaag
     9001 gatgtgatgg agatttcttt catggtcgtg ccttctttcc gcttttattt tattgtacat
     9061 catccgcccc agccgcgcaa caaaaattcc cgccgggcgg gcacccggcg ggtagaggaa
     9121 tcattcaaac tcgagggcgt aggaactttt tgtccgtttt cctattgcta tatcgtctat
     9181 ttttaggatt cttttgagta tagtttgtgg atttttagaa tttttctcac aaacggagtc
     9241 aatggggagt cagtatctat taatgtacta aagttttccg ttgctttttc aggtaaacat
     9301 tatgccccat tcaatttctt ataatctttt agcaactccc aaatccgcat tttacgattt
     9361 taatccggat ttcttgcgga tgccgcctgc gagcagtata atattgataa cgttattagc
     9421 gataacatct cttactacga tgctggaaag aatggaaaat caagggctga taagccgtgt
     9481 tcagtctgaa a
//
LOCUS       DBMDSQ010000041         9153 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_2108_length_9153_cov_1.718515, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000041 DBMDSQ010000000
VERSION     DBMDSQ010000041.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 9153)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9153)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9153
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2108_length_9153_cov_1.718515"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1153
                     /locus_tag="ACZPAX_02705"
     CDS             <1..1153
                     /locus_tag="ACZPAX_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015536618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR1112840.1"
                     /translation="GTSLEAFHEKYPNITVSPTYGAWSGWEEKQSTALAADQGSDVMQ
                     VNWNWLYQYSPTGEKFVDLREYSDVIDLSQWSDAAKNACTVADSLQAVPISMTGRIFY
                     WNMNTFKQAGIEQVPASYDDLIAAGKAFQEKLGDDYYPLAIGQYDRMILMTFYLESKY
                     GKAWVENNECQYSEEEVVDGLNFIKSLEDNHVIPTRPTMIAAGFDSIDKSQEWIDGKY
                     AGIFEWDSSANKYQSALADNSGFTVGEEIKFGDKANGGFSKVSMGMAITSSCQHPVEA
                     AALIDFLWNGEGAAIMGSECGIPASTAGLAAAQAADAVKPLVLEANTKVLAFVDMPLD
                     PYFESSKLKDTTDGVYTDVFDGFSYGDYDAEEAAEILLDGVSGVLSANA"
     gene            1275..2183
                     /locus_tag="ACZPAX_02710"
     CDS             1275..2183
                     /locus_tag="ACZPAX_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015565363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR1112841.1"
                     /translation="MSSAPKTTQRTPFKRWCDENTGLFFLIPWILGFLIFKAYPFASS
                     LVYSFTDLNFFKGISQYGVMNYVEIFTSRKTVSVLLTTVKYAFITVPLKLVFALFIAY
                     ILNFKIKFVNLFRTVYYIPSILGGSVAIAVLWRALFKDTGVVNTILGFFGIAGPDWLA
                     DKTWALFVICLLRVWQFGSAMVLFLAALKGVPAALYEAATIDGATKTRQFFSITIPMI
                     TPVIFYNLVTQIAQAFQEFNGPYIITQGGPRNATTLVSLLVYNYAFKSNEMGMACALA
                     WVMFIAVALLTVIAFTSQKHWVYYAD"
     gene            2197..3057
                     /locus_tag="ACZPAX_02715"
     CDS             2197..3057
                     /locus_tag="ACZPAX_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005942942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR1112842.1"
                     /translation="MKLSTAHKIGRFFQYFVLILVGVIMIYPLVWMVGATFKSNAEIF
                     SGIGFLTANPTLQGYIDAVTQNYGGDISIWRAFINTYSFVIPKVIFTVISSVIAAYGF
                     SRFKFKGRDMLFGIMISTLFLPQVVLNVPQYLMYNSFGWVNSPFYLPLWVPTLFATET
                     YFVYQLVQFMRSIPHDLDEAAAIDGCGPVKILYKIIAPMLSPSLVACGLFQFMWSCND
                     YMGPLLYVQTPSKYPMSIFVKLSMDADSGFNWNRILALSLISIIPQLIVFFCAQDAFI
                     DGISAGAVKG"
     gene            complement(3301..4164)
                     /locus_tag="ACZPAX_02720"
     CDS             complement(3301..4164)
                     /locus_tag="ACZPAX_02720"
                     /inference="COORDINATES: protein motif:HMM:NF014376.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1112843.1"
                     /translation="MVSEYVYSPHVDFFIDHVDRDTGYEMPSFHIHHKYEIYYEIEGS
                     RRYFIEDAAYIVNAGCVVLIGENQIHKTASLGDTASSRIVANFSREYLGKITDIFPEV
                     DFFSFLSEEHNHLLSNITVKQQNHIHNLLQQLLALQEETSPESDAMRKMLLATLLLEL
                     KDLCRQQQEQGGENGRVSNHIVDQIQAYIAEHYAEKLTLTGIASQFYISPYYLSRLFK
                     KSINLSLIEYINGVRIKAAQNLIEKSNESISDIAEKTGFMTTAHFRRVFKDATGLSPQ
                     QYRQYYKRVNK"
     gene            4296..5849
                     /locus_tag="ACZPAX_02725"
     CDS             4296..5849
                     /locus_tag="ACZPAX_02725"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005927110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HIR1112844.1"
                     /translation="MKITLVRSMARSAVFELVNSGCYRAPAPYTVALDGDAVYDGDTN
                     VFSLYSLEPGRSYTVAVTLDGVTDTLEFTTAEESFFVDASRCGLVADGVTDNTAKLQA
                     ALSTCPVGGTVYVPAGTYRTQSLFLQSNTTLYLEKGCTLLGGTKRTDYPILPGVLPCH
                     NERDEHYLALWEGNPLDSFAGLINVINAENVTITGEGTIDANAQNGDWYQNPKKKNIA
                     YRPRLFFTNGAKNVVLHGVQVCNSYSWTIHPTYSENVDILNIRIRNSSSSPNTDGIDP
                     ESCKNVNIIGNHIHVGDDCIALKSSKLFLGMKRHIPCENVVIRNCCLSRGHGGLVIGS
                     EMSGGVKNVTVTQCLMDHTDRGLRVKTRRGRGKYAVIDGLVFRDVVMDGVLTPFVINM
                     FYFCDPDGRSDYVQTHDPLPVDEMTPTLGTLEMENITATDAQYAGCWFSGLPEQPIGG
                     VKMRNVKISFAPDAKAGQAAMMCGADASCKVGIHAENVRKIELHNVEITGYEGERLQF
                     THVDSYEED"
     gene            5853..7004
                     /locus_tag="ACZPAX_02730"
     CDS             5853..7004
                     /locus_tag="ACZPAX_02730"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005934947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88/105 protein"
                     /protein_id="HIR1112845.1"
                     /translation="MTHEEILSTLGHYVDYLIAGSTAEAPLWNIEKVRSGKPNKWNYI
                     DGCMITACLSLYKTTGDEKFLTFSKNFLDYFVKDHGIIETYDPEEYNLDNVNQGKNLF
                     TLYDLTGDQRYRDAIETIRGQLETQPRTKEGNFWHKKIYPNQVWLDGTYMAQPFYMEY
                     ETRCNEMHGCIDSYKQFMNIQKHMKDPKTGLYYHGYDESREMYWADPVTGCSANFWLR
                     AMGWFLVAMVDVLERMDEMLYYEYRAIMSMLKDAVDAMIAFQDAESGMFWQVIDKADV
                     EGNYLETSGSALFAYAVLKGVRLGYLPKRYAAYGEKAFYGTCDRHLGVGADGALQLGG
                     ICLVAGLGGATRRDGSLAYYFSEPIVENDAKGVGPLLLAYTEILAAQKQ"
     gene            7017..7844
                     /locus_tag="ACZPAX_02735"
     CDS             7017..7844
                     /locus_tag="ACZPAX_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015563957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HIR1112846.1"
                     /translation="MPKFIVGARLHDYGKGTPDELFAKVSADGFAAVQLAYKKCVPTV
                     KSYADVTDALVADTIAAEKAHSIQVAVLGTYVELAINDESRLQNAADFKSQLAVCKAL
                     GAGCIGTETTAMSKQPAGTTREEAQELLCRSLAEILPTAEELGVTVGIEPVTLHSMNS
                     PAATRHILDMMCSPNLKVIFDMSNLVNAENVHDQARIWNDMGELLGEEIVAVHFKGQA
                     FDAEGGLLHTSLEESRTDYAGAFAMLRQLPQATLPVLREEAVPARAASDIAFMKGFF"
     gene            7844..8818
                     /locus_tag="ACZPAX_02740"
     CDS             7844..8818
                     /locus_tag="ACZPAX_02740"
                     /inference="COORDINATES: protein motif:HMM:NF013276.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HIR1112847.1"
                     /translation="MTDIQRKTLVVDEGGTGDYRTLTDAVAACADTPNEPVRFYLKHG
                     IYEERPFIELADYIIEGEYRNATVLTASVGGRDPWPGESKTGTFRSETLFLGGGRAVV
                     RNLTVQNNAGDGAKAGQALAVYADASRVLMEDVNLYGNQDTLFTAPLPLKEREEHGFR
                     GPRENAPRLDTVQYYKNCTIRGNIDFIFGGANAVFDRCRIEPWHHATGTCYITAPSTP
                     AGKPGYLFVNCTVQGSAAPGSVYLGRPWRADAACYWLDCVLSDEVCTDGWDNWRDPEN
                     EKTARFGEYGSTGPGSAAQRAFGSVDNDTEANAQREMLARVRAEFGVE"
BASE COUNT         1880 a         2780 c         2579 g         1914 t
ORIGIN      
        1 gggtacttct ctggaggctt tccacgagaa gtaccccaac atcaccgtct cccccaccta
       61 cggcgcatgg tccggctggg aagagaagca gtccaccgcg ctggccgctg accagggttc
      121 cgatgtcatg caggtcaact ggaactggct gtaccagtac agccccacgg gtgagaagtt
      181 cgtcgacctg cgcgagtaca gcgatgtcat cgacctgagc cagtggagcg atgccgccaa
      241 gaacgcctgc accgttgctg acagcctgca ggctgtgccc atctcgatga ccggccgtat
      301 cttctactgg aacatgaaca ccttcaagca ggccggtatt gagcaggttc ccgcctccta
      361 tgatgatctg attgccgccg gcaaggcctt ccaggagaag ctgggcgatg attactatcc
      421 tctggccatc ggtcagtatg accgcatgat cctgatgacc ttctacctcg agtccaagta
      481 cggcaaggcc tgggtcgaga acaacgagtg ccagtacagc gaggaagagg tcgttgatgg
      541 cctgaacttc atcaagtccc tcgaggacaa ccacgttatc cccacccgtc cgaccatgat
      601 cgctgctggc ttcgactcta tcgataagtc tcaggagtgg atcgacggca agtacgccgg
      661 catcttcgag tgggattctt ccgccaacaa gtaccagtct gctctggccg acaactccgg
      721 cttcaccgtt ggtgaggaga tcaagttcgg cgacaaggcc aacggcggct tctccaaggt
      781 ctccatgggc atggccatca cctcctcctg ccagcatccc gttgaggctg ctgccctgat
      841 cgacttcctg tggaacggcg agggcgctgc catcatgggt tccgagtgcg gcattcccgc
      901 ctccaccgcc ggtctggctg ccgctcaggc tgctgacgct gtgaagcctc tggttctcga
      961 ggccaacacc aaggttctgg ccttcgtcga tatgccgctt gacccgtact tcgagtccag
     1021 caagctgaag gacaccaccg acggcgttta caccgatgtg ttcgacggct tcagctacgg
     1081 cgactacgac gctgaggagg ctgccgagat cctgctggac ggcgtttccg gtgtgctgag
     1141 cgccaacgcc taagagcaat cgaaatcctt taaaaagtaa agtttagccg gccccagccc
     1201 cgttatcacg catggcgggg ccggttattc atgttaaaac gcaatgcagc aatttgtgac
     1261 aaggaggttt ttggatgagc tctgcaccca aaacgaccca gcgcacaccg tttaagcgtt
     1321 ggtgtgacga aaacactggc ctgttcttcc tgattccgtg gattctgggc ttcctgattt
     1381 tcaaagccta tccgtttgcc agctctctgg tatacagctt tactgacctg aacttcttca
     1441 agggcatcag ccagtacggc gttatgaact acgttgagat tttcaccagc cgcaagaccg
     1501 tttccgtttt gctgaccact gtgaaatatg ccttcatcac cgtgcccctc aagctggtgt
     1561 tcgcgctgtt catcgcctac atcctgaact tcaagatcaa gttcgtcaac ctgttccgta
     1621 ccgtttacta cattccgtcc attctgggcg gctctgtggc catcgccgtt ctgtggcgcg
     1681 ccctgttcaa ggacaccggc gttgtcaaca cgattctcgg cttctttggc attgccggtc
     1741 ccgactggct ggctgacaag acctgggcgc tgttcgtcat ctgcctgctg cgtgtgtggc
     1801 agttcggctc cgccatggtt ctgttcctag ccgctttgaa gggcgtgcct gccgccctgt
     1861 acgaggccgc caccattgac ggtgctacca agacccgcca gttcttctcg atcaccattc
     1921 cgatgatcac ccccgttatc ttctacaatc tcgtcaccca gatcgcacag gcgttccagg
     1981 aattcaacgg cccctacatc atcacccagg gcggcccgcg taacgctaca acgcttgtct
     2041 ccctgctggt ttacaactac gccttcaagt ccaacgagat gggcatggcg tgtgcgctgg
     2101 cctgggtcat gttcatcgcc gttgctctgc tgactgtgat tgctttcacc agccagaagc
     2161 actgggtcta ctacgctgat taaggaggaa cgaaaaatga aactttctac cgcccataaa
     2221 atcggccgtt tcttccagta ctttgtgctg atccttgtcg gcgttatcat gatctacccg
     2281 ctggtctgga tggtgggcgc caccttcaag tccaacgccg agatcttctc cggcatcggc
     2341 ttcctgactg ccaatcccac gctgcagggc tacattgacg ccgtgaccca gaactacggc
     2401 ggcgacatca gcatctggcg cgccttcatc aacacctaca gcttcgttat ccccaaggtc
     2461 atcttcaccg ttatcagctc tgtcattgcc gcttacggct tcagccgctt taagttcaag
     2521 ggccgcgata tgctgttcgg catcatgatc tccacgctgt tcctgcccca ggtcgttctg
     2581 aacgttcccc agtacctgat gtacaactcc ttcggctggg tcaacagccc cttctacctg
     2641 cccctgtggg tgcccaccct gtttgccacc gagacttact tcgtctatca gctggtgcag
     2701 ttcatgcgca gcatccccca tgacctggac gaggcggccg ccatcgacgg ctgcggcccg
     2761 gtcaagatcc tgtacaagat cattgccccg atgctgagtc cctctctggt ggcctgcggt
     2821 ctgttccagt tcatgtggag ctgcaacgac tacatgggtc ccctgcttta cgtccagacc
     2881 cccagcaaat acccgatgtc cattttcgtc aagctgtcca tggacgctga cagcggtttc
     2941 aactggaacc gtattctggc attgtccctc atctccatca tcccgcagct gatcgttttc
     3001 ttctgcgcgc aggatgcttt catcgacggt atctccgccg gtgccgtgaa gggctaatcg
     3061 ccattttggc agcccgaaaa cccacttaaa ccacggctgc cccgcaccac aaccaggggg
     3121 tacggggcag cctttctttt gaaaatgaca ctcccctccc ctgccagggg gacggagttt
     3181 tactgataga tttgcagggg acggcgtccc cagcgccccg cgggcggatg caggcatccg
     3241 cccctgcaac ggtgcaaaca aaaaggccgc cccatatcag ggcggcggat gtttcaaacc
     3301 ttacttattc actctcttat aatactgccg gtactgctgc ggggacagac ccgtggcatc
     3361 cttgaacaca cggcggaaat gggcggtggt cataaagccg gtcttttccg caatgtcgga
     3421 aatgctctcg ttcgatttct cgatgagatt ctgcgccgcc ttgatacgca cgccgttgat
     3481 atactcgatc agactcaggt tgatggactt tttgaagagt ctgctcaggt agtaggggct
     3541 gatatagaac tggctggcaa tgccggtcag ggtcagcttc tcggcgtaat gctcggcgat
     3601 gtaggcttga atttggtcca cgatatggtt gctgacgcgg ccgttctcgc cgccctgctc
     3661 ctgctgctgg cggcagagat ccttgagttc cagcaaaagc gtggccaaca gcattttgcg
     3721 catggcgtcg gactccgggg aggtttcttc ctgcaaagcc agcagctgct gcaatagatt
     3781 gtgaatatgg ttctgctgct tgaccgtgat gttggagagc agatggttgt gctcttcgct
     3841 caagaacgag aagaaatcca cctccgggaa tatgtcggtg attttgccca ggtattcccg
     3901 gctgaagttc gcaacaatgc ggctggaggc cgtgtccccc agcgaggcag tcttgtgaat
     3961 ctggttctcg ccaatcagca caacacagcc tgcgttgacg atgtaggcgg catcctcgat
     4021 aaaatagcgg cggctgccct caatctcata gtaaatctca tacttatggt ggatgtggaa
     4081 actgggcatc tcgtagccgg tatcgcggtc aacgtggtca ataaaaaagt ccacatgggg
     4141 cgagtagaca tattccgaaa ccataacgct tgctccttgc tgccgcggtg ccatgcgccc
     4201 ggcgattggt tcttgttcta tttactagaa tagcacaatt tttgctgttt ttcaaactat
     4261 ttttatacaa atttttatta tttgggggtt ttcacatgaa aatcacgctt gtacgttcta
     4321 tggcgcgcag tgcggtattt gagttggtca attccggctg ctaccgcgca ccggcccctt
     4381 acacggttgc cctggatggt gatgctgtct atgatggtga caccaacgtt ttctcgctct
     4441 actccctgga gccgggccgc agctacacgg tagccgtcac cctcgacggt gtgaccgata
     4501 cgctggaatt taccaccgcc gaggagagct tctttgtgga tgccagccgc tgcggcctgg
     4561 tggcggacgg cgtgaccgac aacactgcca aattgcaggc ggcgctgtcc acctgcccag
     4621 tcggcggcac ggtctatgtg cccgccggta cttaccgcac ccagagcctg tttttgcaga
     4681 gcaacacaac gctctacctc gaaaaaggct gcacgctgct gggcggcacc aagcgcaccg
     4741 actatccgat tttgcccggt gtcctcccct gccacaacga aagggacgag cactacctgg
     4801 ccctgtggga gggcaacccc ctggacagct ttgcggggct tatcaatgtc atcaacgccg
     4861 agaatgtgac catcaccggc gagggcacga ttgacgccaa cgcccagaac ggcgactggt
     4921 accagaaccc gaagaagaag aatattgcct accgcccccg tctgttcttt acaaacggcg
     4981 ccaaaaacgt ggtgctgcac ggtgtgcagg tctgcaacag ctactcttgg accatccacc
     5041 cgacctactc ggagaatgtg gacattctga acatccgcat ccgcaacagt tccagcagcc
     5101 ccaacactga cggcatcgac cccgagagct gcaagaatgt caatatcatc ggcaaccata
     5161 tccatgtggg cgacgactgc attgcgctga aatccagcaa gctgttctta gggatgaagc
     5221 gccatatccc ctgcgagaat gttgtcatcc gcaactgctg cctgagccgc ggccacggcg
     5281 gcctggtcat cggcagtgag atgagcggcg gcgtcaagaa tgtcaccgtt acccagtgcc
     5341 tgatggatca cactgaccgc ggcctgcgcg tcaagacccg ccgtgggcgc ggcaagtatg
     5401 ccgtcattga cgggctggtg ttccgcgacg tcgttatgga cggcgtgctg acgccctttg
     5461 tcatcaatat gttctatttt tgcgacccgg acggccgcag cgactatgta cagacccacg
     5521 acccgctgcc ggtggacgag atgaccccga cactgggcac gctggaaatg gagaacatca
     5581 ctgccaccga tgcccagtac gcgggctgct ggttcagcgg cctgccggag cagcccatcg
     5641 gcggcgtaaa gatgcgcaat gtgaaaatca gctttgcgcc cgacgccaag gcgggccagg
     5701 ctgccatgat gtgtggtgcg gatgccagct gcaaggtggg catccacgcc gaaaatgtgc
     5761 gcaagatcga gctgcacaac gttgaaatca ccggctacga gggcgagcgc ctgcaattca
     5821 cccacgtaga cagctatgag gaggactaag ccatgaccca cgaagaaatc ctatccaccc
     5881 tgggccatta tgtggactac ctgattgccg gttccaccgc cgaggccccg ctgtggaaca
     5941 ttgaaaaagt gcgcagcggc aagcccaata agtggaacta catcgacggc tgcatgatca
     6001 ccgcctgcct aagcctgtac aagaccaccg gtgacgagaa gtttttgacc ttctccaaga
     6061 attttctcga ttacttcgtc aaggatcacg gcatcattga gacctatgac cctgaggaat
     6121 acaacctaga caatgttaat cagggcaaga acctgtttac cctctacgac ctgaccggcg
     6181 accagcgcta ccgcgacgcg attgaaacga tacgcggcca gttggagacc cagccccgca
     6241 ccaaggaagg caacttctgg cacaaaaaaa tctaccctaa ccaggtctgg ctggacggca
     6301 cctacatggc ccagcccttc tacatggagt acgagacccg ctgcaacgag atgcacggct
     6361 gcattgacag ctacaagcag ttcatgaaca tccagaagca catgaaggac cccaagaccg
     6421 gcctgtacta ccacggctac gacgagagcc gcgaaatgta ctgggccgac cccgtcaccg
     6481 gctgcagcgc caacttctgg ctgcgcgcca tggggtggtt tctggtggcg atggtggatg
     6541 tgctggagcg catggacgaa atgctgtact acgagtatcg cgccatcatg tcgatgctga
     6601 aggacgccgt ggacgcgatg atcgcgttcc aggacgccga gagcggtatg ttctggcagg
     6661 tcattgacaa ggccgacgtc gagggcaact acctggagac ctccggcagt gcgctgtttg
     6721 cctacgcggt gctcaagggc gtacggctgg gctacctgcc taagcgctat gccgcctacg
     6781 gtgagaaggc cttctacggc acctgtgacc gccacctggg cgtgggcgcg gacggtgcgc
     6841 tgcagttggg cgggatctgc ctggtggcag gtcttggcgg tgcgacccgc cgtgacggca
     6901 gcctggccta ctatttcagt gagccgatcg tggaaaacga cgccaagggt gtaggcccgc
     6961 tgctgctggc ctacacggag atcctggccg cccaaaagca gtaaggaggg gtttgtatgc
     7021 cgaaatttat cgtgggtgcc cgcctgcatg actacggcaa gggcacgccc gacgaactgt
     7081 ttgccaaggt ctctgccgac ggttttgccg ccgtacagct ggcctacaaa aagtgcgtgc
     7141 ccacagtcaa gagctatgcc gacgtgaccg acgcgctggt ggccgacact attgccgccg
     7201 agaaagcaca cagcatccag gtggccgtgc tgggcaccta tgtggagctt gccatcaacg
     7261 atgagagccg cttgcagaac gcggcagatt tcaagagcca gctggccgtc tgcaaggcac
     7321 tgggggccgg gtgtattggc acggagacga ccgccatgtc caagcagccc gccggtacga
     7381 cccgcgagga agcccaggaa ctgctttgcc gcagcctggc tgagattctg cccacggcgg
     7441 aggaactggg cgtcacggtg ggcattgagc ctgtgacgct gcacagcatg aactcccccg
     7501 ccgccacgcg gcacattctg gacatgatgt gcagcccgaa cctgaaagtc atctttgaca
     7561 tgagcaacct tgtcaacgcc gagaacgtgc acgatcaggc ccgcatctgg aacgacatgg
     7621 gtgagctgct gggcgaggaa atcgtcgccg tacacttcaa aggccaggcg tttgacgccg
     7681 agggcggcct gctgcacacg agcctggaag aaagccgcac cgactacgcc ggggccttcg
     7741 ccatgctgcg ccaactgccc caggccaccc tgccggtgct gcgcgaagag gccgtacccg
     7801 cccgcgcggc tagcgacatt gcattcatga aaggattctt ttgatgactg acattcagag
     7861 aaaaacactg gtagtggacg agggcggcac cggcgattac cgcacgctga ccgacgccgt
     7921 ggccgcctgt gccgatacgc ccaatgagcc ggtacggttc tacctcaagc acggcatcta
     7981 tgaggaacgg ccctttattg aactggccga ctatatcatc gagggcgaat accgcaatgc
     8041 gaccgtactc acagcctctg tgggcggccg tgatccctgg ccgggcgaga gtaagaccgg
     8101 aaccttccgc agtgaaacgc tgtttctggg cggcggcagg gccgttgtgc gcaacctgac
     8161 tgtgcagaac aatgccgggg atggtgcaaa ggcagggcag gcgctggctg tctacgccga
     8221 cgccagccgc gtgctgatgg aggacgtgaa cctttacggc aaccaggata cattgttcac
     8281 tgctcccctg cccctgaaag agcgggagga gcacggcttc cgcggcccgc gggaaaacgc
     8341 accccggctg gacaccgtgc agtattacaa gaactgcacg atccgtggca acattgactt
     8401 tatcttcggc ggcgcgaacg cggtgtttga ccgctgccgc attgagccgt ggcaccacgc
     8461 caccggcacc tgctacatca cagcccccag cacgcccgct ggcaagcccg gctacctgtt
     8521 tgtgaactgt accgtacagg gcagcgccgc gcccggcagc gtctatctgg gccgcccctg
     8581 gcgggctgac gcggcctgct actggctgga ctgcgtgttg agcgacgaag tctgcaccga
     8641 cggctgggac aactggcgtg accccgagaa cgaaaagact gcccgcttcg gcgagtacgg
     8701 ctccaccggc cccggcagcg ccgcccagcg tgcctttggc agcgtggaca acgacaccga
     8761 ggccaacgcc cagcgggaga tgctggcccg cgtaagggct gaatttgggg tagaatagcc
     8821 tgtatagaaa aagcggcaga ccgtgcatag tctgccgctt ttctttatgc cttccccctt
     8881 ggggctgcgc ccgcaggcgc gtgtcggagc gcaaccgccg taggcggctc ttagcgcgta
     8941 gactgaaggt ggccccgcag ggccggatga ggggcgagct tgcggctgca tcccattcaa
     9001 aggttgcctg gcaagcccgc tcctcatcag tccgcttcgc ggacagcttc tcccaaggga
     9061 gaagccttat ttagttggcg ctcgtcacgc cgccctcgac gacaaggctg ttctcgtagt
     9121 cggtgccata ggtgtcaaca agggtcgcct cat
//
LOCUS       DBMDSQ010000042         8710 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_2219_length_8710_cov_2.156813, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000042 DBMDSQ010000000
VERSION     DBMDSQ010000042.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 8710)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8710)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8710
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2219_length_8710_cov_2.156813"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..858)
                     /locus_tag="ACZPAX_02745"
     CDS             complement(<1..858)
                     /locus_tag="ACZPAX_02745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1112848.1"
                     /translation="MKKQFSKIFAVSLSVAALAVSVPVSAFAKSSKPLSYLYTYQLTF
                     DGYDTMSLSPDDTGTITAPALPEGAQYWQCNGKHVMPGEQFSYDQLDDWLCPSVDIDD
                     GTGTHRTTGAYFMAITGDTAYVHYYADDTAVKTDAVFDGGVSEVPSTVNGKAVAYWVT
                     ADGGLSIYTPATLSYDELFWHIHVEEMGNNLNLYAVFGDGSTPAPAPQPVVTETPAAS
                     TEDNTPAVDTRTEQQKEIDEAIANGTWGIEYTTCQKCGYHNWTRQGNVYVCDTCGNTT
                     TTVVGPKGVK"
     gene            complement(983..2323)
                     /locus_tag="ACZPAX_02750"
     CDS             complement(983..2323)
                     /locus_tag="ACZPAX_02750"
                     /inference="COORDINATES: protein motif:HMM:NF024637.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HIR1112849.1"
                     /translation="MICTHCGKEIQNGITFCPYCGSTVNNAKENTKMKKHNPAKVLVS
                     IAAVAVVAAGGWAFANRTPTIDVSKYMTLSADGYNTVGKLNIDFDTEKLEKDYGKQIT
                     TRFKKQMKNLKDDTYGLSSLTASLYDGYEADLFAETCATGSADKTKNLSNGDVVTYTW
                     DDNSDEAEEAFGVKVKYTDITYTVSGLASVNTFDAFDGVDVEFSGISPDGRATVNSLP
                     TAAEAQGLYYTLDENSGLSNGDTVTLTVHSNRDDFSDCIDKYGAMPQVTEKTFTVEGL
                     NEYVTSADTLSDSVLVSLQNQAEDVFKSYAAQRFSNGQTFKGMTYLGNYILTPKNKDS
                     WGDKDRIVLAYQVTVHHDYTSELNTTYDADDSFFWYITFNNVSKDADGNIASGLNDYD
                     TPTTFVKIDSGVQKYSFSSSTETWEYYGYASLDSLYNAAVNQYVENYNHQDNVA"
     gene            2697..4880
                     /locus_tag="ACZPAX_02755"
     CDS             2697..4880
                     /locus_tag="ACZPAX_02755"
                     /inference="COORDINATES: protein motif:HMM:NF025053.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HIR1112850.1"
                     /translation="MHPHTATSAILRRGLTIRDLQCPPADADTVTSITIDDTIRAAKG
                     NFGFVYAATGDRELYRECLLAESYAKTQYVSCAIHLRRAFELLAFYTVGRALCTVTPG
                     LTMPEAKAHAKQFYESDPRCFNPKLQLSLVLAGLGYIDVVFNERFVFSDNPAYPGCKK
                     MVDMLFSLYSDASGMAHTGRDGGHRDCSWLLRRFYTAAGMLLQVDVPAADFFPAMDRF
                     LHRYDASVAPLENYYPLSAAVCRQMDVCAAGDSLLCVTDEAAPTFYIAKHVTDDPAVG
                     RDIGSLQSLWRASEQTPENVLIDVGPLGTGEDRYHLFRIVGRPYALSAKVLAGLNSTQ
                     KRQIINGIVGSIYTLHHFMPPLPHRGLRPGCYYVCRQGANCRAVLALFSTIKDNTAEK
                     TVYGELNSLLQNVTDRMYIAPELSGGSARPGELTKADIYSLGRLIAFVYTGEPRADLD
                     DLRIPCAMVELVQKLTDPDPARRPDIDAVRARLENGGGQRLVYGAAACQGTRPRQEDV
                     LLANGTLTARPDYTLADTAPCPAAFAVLDGIGGGYGGAEVAHAAAEALDRAAAPYLHM
                     ELADYTQPLAAIFADAERETKRFMAENGYTRSGASAALVIAASRAVYTANLGDSRVYR
                     YTHGALTQLTCDHRFALGGRHELYQYLGASDEDTEISPTIGKIDRVAGERLLLCTDGV
                     SGKLSDEELAAMLTAHPDAGDAAGAIIAAVKSVATDNATVLVVDL"
     gene            5041..6927
                     /locus_tag="ACZPAX_02760"
     CDS             5041..6927
                     /locus_tag="ACZPAX_02760"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF012298.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HIR1112851.1"
                     /translation="MDTNTFNNLISQTWPDLHAASTLGGGTFGTVYACTKTDAVTGVT
                     QKEAVKVVRVDFTPEDRANAEEEGIPFADYYRAVKEKRLQEIRWMVALKSPHIVHING
                     YTAIEEPDHSALYILIRMDCLTALDQLRPAHLDDTPEQAAALAEKVALDICDALRVCH
                     QNGVLHRDIKPANILCSDAGDFYLGDFGISKGTAQQASMTSSGTLEYAPREVMTGQYD
                     HRADLYSLGLVLYALVNHWRGPFLPAYPAPITPGNRNQAQYARMNGTPLPPPDNCPTA
                     LWAVIARLCAFAPEERFDSAEAVLAALCDPGTPAPAPAQHRAHPNLPRIRHPRRLAAV
                     VLVFAVAVGVGVGLYRRQQMAAPDISLGAGRDSTDAALAQASDLLQGNENFMTAHNLQ
                     FCSDRNVTIPGGALVYLTDPAKIESDYPGYTTAADADVEAGDLTLTFGDVTVEDNDDG
                     TVTYTIQLTERADITQRRTQDAPIYGVGARFATVLPFDAATGQVFPSLTEDWTPHRAY
                     TVQMTATVDGHTYEMPINRDTTWQMESADGDVAATEQVDDLPGYGMPGLILRAAYVKQ
                     QTIMIKAPAASDLGLLVCTAPYDGYTEGDYENTPPVQALADTPGFAGYDKYWYFDLNE
                     LTKE"
     gene            6933..>8710
                     /locus_tag="ACZPAX_02765"
     CDS             6933..>8710
                     /locus_tag="ACZPAX_02765"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF012298.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HIR1112852.1"
                     /translation="MLPLSSLISQKWLDIQIDAPLGSGACGTVYACTKKSAVTGIESR
                     EAVKVVRVEFSAAAHRQAEEEGIPFDEYYARIKAEKSREIELLVNLKSPHIVHINEFD
                     AVEEPDHSAFYLLIRMDLLQALTAFRVDHLTDTPEQAGAYARKVMLDICDALRVCHEN
                     GICHRDIKPANILYGATGDFYLGDFGVSQYTAAPDATLTSSGTARYAAPEQLIGRADP
                     RSDLYSLGLVLYELTNHWRGPFLPAYPAKLTAEDRYNAQCKRLAGGALPPPDNCPPAL
                     AAVILKLCAPDPGDRYQSVQEVLDALSPAAAPATMQKKKPRRLWIGVGLVVAAVAVAW
                     LTARVGGWKYYPNPGTVVETRDTTVWDYARKNGITVSDATHVGFNGGAYNYPCQTSGF
                     YEYFEPDGVTDWGATRLSATVTGAKKSDPDADDNVTYTVTCAARARMDYTVAEGVEPF
                     LPGLDLRVLELMDLRSGYTFPLGNLNGPQETTPRSVEVSGDYTFSYQGQTFPVQVSRT
                     EQWEYTLPDYTVPGAYQTQVVVYYAYTVTVPQTLDSLALYIDTTPYTGYSRTLDTRSK
                     TLDQEDSFAGFENYEFFALDDVVSDFA"
BASE COUNT         1602 a         2736 c         2677 g         1695 t
ORIGIN      
        1 cttcacaccc ttggggccga cgactgtggt ggtcgtgttg ccgcaggtgt cgcaaacata
       61 gacgttgccc tggcgggtcc agttgtgata accgcacttc tggcaggtgg tgtactcgat
      121 gccccaggtg ccgttggcaa tggcctcatc gatctccttc tgctgctcgg tgcgggtatc
      181 cacagccggg gtgttgtcct cggtggacgc ggcgggcgtc tcggtcacga ccggttgcgg
      241 cgcgggggcc ggggtggagc catccccaaa gacggcgtag aggttcaggt tgttgcccat
      301 ctcctcgacg tggatgtgcc agaacagttc atcgtagctc agggtcgcgg gggtgtagat
      361 gctgagcccg ccgtctgccg tgacccagta ggccaccgcc ttgccgttca cggtgctggg
      421 cacctcgctg acgccgccgt caaacactgc gtcggtcttg accgctgtgt cgtcggcgta
      481 gtagtggacg taggccgtat cgccggtgat ggccataaag tacgcgccgg tggtgcggtg
      541 ggtgccggtg ccgtcatcga tgtccacgct ggggcagagc caatcatcca gctggtcata
      601 gctgaactgc tcgccgggca tgacgtgctt gccgttgcac tgccagtact gggcaccctc
      661 cggcagggcg ggtgcggtga tggtgccggt atcgtccggg gagagggaca tcgtgtcgta
      721 gccgtcgaag gtgagctggt aggtgtacag gtagctcagc ggcttgctgg acttggcgaa
      781 agcagagacc ggcacgctga cggccagcgc ggcgacactg agggaaacgg cgaagatttt
      841 ggaaaattgc tttttcatga taaagttcct ttctatgtag aaaaattatg ggcggagacg
      901 ttccgcccgc ggaaacgtga ggggcggcgg cctggggaag gtgggctgcc aaggggtgcg
      961 gtgggaaagg tgcatgggtg atttaggcaa cattgtcctg gtggttgtag ttttccacgt
     1021 actggttgac ggcggcattg tacaggctgt ccagagaggc gtagccgtaa tactcccagg
     1081 tttccgtgct gctgctgaag ctgtacttct gcacgccgga atcaatcttc acaaaggtgg
     1141 taggcgtgtc gtagtcgttc aggccgcttg cgatattgcc gtcggcgtcc ttgctgacgt
     1201 tattgaaggt gatgtaccag aagaaggagt cgtccgcgtc gtaagtcgta ttcagctcgc
     1261 tcgtgtaatc gtggtggaca gtcacctgat acgcgaggac gatgcggtcc ttgtcgcccc
     1321 agctgtcttt gtttttcggg gtcaggatgt agttgcccag gtaggtcatg cccttgaagg
     1381 tctgaccgtt ggaaaagcgc tgcgcagcgt aagatttaaa cacatcctcc gcctggttct
     1441 gcaagctgac cagcacggaa tcggagagcg tgtccgcact cgtcacgtac tcgttcagcc
     1501 cttccaccgt gaaggtcttt tcggtcacct ggggcatcgc gccgtacttg tcaatgcagt
     1561 cgctgaaatc gtcgcgattg ctgtggacgg tcagggtcac ggtgtcgccg ttggacaggc
     1621 cgctgttctc gtccagggtg tagtacaggc cctgcgcctc ggcggcggtg ggcaggctgt
     1681 tgacggtggc gcggccgtcg ggagagatgc cgctgaactc cacatccacg ccatcgaagg
     1741 cgtcaaaggt gttcacgctg gccaggccgg atacggtgta ggtgatgtcc gtgtacttga
     1801 ccttgacgcc gaaggcctcc tcggcctcgt cggagttgtc gtcccaggtg taggtgacga
     1861 cgtcgccgtt ggacaggttc ttggtcttgt cggcgctgcc ggtagcgcag gtctcggcga
     1921 aaaggtcagc ctcgtagccg tcgtacaggc tggcggtcag gctgctcagg ccgtaggtgt
     1981 cgtccttgag gttcttcatc tgctttttga agcgggtggt gatctgcttg ccgtagtcct
     2041 tctcgagctt ttcggtgtcg aagtcgatgt tcagcttgcc gacggtgttg tagccgtccg
     2101 ccgagagggt catgtacttg ctgacgtcga tggtcggggt gcggttggcg aacgcccagc
     2161 cgcccgccgc aacgaccgcc actgcggcga tggaaacgag gaccttagcg ggattgtgct
     2221 ttttcatttt cgtattctcc tttgcattat tgactgtgct gccgcagtag gggcagaaag
     2281 tgatgccgtt ctggatttct ttaccgcaat gtgtgcagat catggtgttt ttcctcctgt
     2341 tttgtagctg acaagtggtt tttgccgtgg tataatgtgg gtaaggtttg cggggttttg
     2401 cttgttttgt ttccttacac ctaagcaata ggggtggggg ttgggaaagt taaggggaat
     2461 tttaaaattt tttgggattt ttttggaaaa gttttaaaag gcaaggttta tcgctgccca
     2521 acaccgggca aaaccccttc aggtctcccc tcacccatcc acatcccccg gaatgctgcg
     2581 ggccgggcat gcccggcccc tacatctata caggacttta acactatgca ataacggcac
     2641 acgccctcgg gagggataaa tcccgcccct tcaactctac ccaggaggcc cccttcatgc
     2701 acccccacac cgctacatct gccatcctcc gccgtgggct gaccatccgc gacttgcaat
     2761 gtccacctgc ggatgccgac acggtcacgt ccatcactat cgacgacacc atccgtgcgg
     2821 ccaagggcaa ctttgggttc gtctacgccg ctaccggcga ccgcgagctt taccgggaat
     2881 gtctcttggc cgaaagctac gcaaaaacgc aatacgtcag ctgcgccatc cacctgcgcc
     2941 gcgcatttga gctactggcc ttctacacgg ttggccgggc gctctgcacc gtcacaccgg
     3001 gtctgaccat gcccgaggcc aaggcccacg ccaagcagtt ttacgagagc gacccccgct
     3061 gcttcaaccc caaattgcag ctctccctcg tgctggcggg gttggggtac atcgacgtcg
     3121 tcttcaacga gcggttcgtt ttctccgata accccgccta ccccggctgc aaaaagatgg
     3181 tggacatgct cttctcgctg tacagcgacg cgtctggcat ggcccacacc ggcagggatg
     3241 gcggccaccg ggactgcagc tggcttctgc gccgattcta cacggcggcg ggaatgctac
     3301 tgcaagtcga tgtgcctgca gcggactttt tccccgccat ggatagattt ttacacaggt
     3361 acgatgcttc tgttgcgccg ctcgagaact actatcccct ctccgccgcc gtctgccgtc
     3421 agatggacgt ctgcgcggcg ggcgacagcc tgctctgcgt caccgatgaa gccgcgccta
     3481 ccttttatat agcgaagcac gtcacagacg atccggccgt cgggcgggat atcggctcgc
     3541 tgcaatcgct ctggcgcgcg tcggagcaga cgcccgagaa cgtcctgata gacgttggcc
     3601 cgctgggcac gggcgaggat cgctaccacc tgttccgcat cgtggggcgg ccctacgcgc
     3661 tttcggccaa ggtgctggcg ggcctgaaca gcacgcagaa gcgtcaaatt atcaacggca
     3721 tcgtgggcag catctacacg ctacaccatt ttatgccgcc gctgccccac cgggggctgc
     3781 gcccgggctg ctactacgtc tgccgccagg gagcgaactg ccgcgctgtg ctggcgctgt
     3841 tctcgacgat aaaggacaat acagcggaaa aaacggtcta tggtgagctg aacagtcttt
     3901 tacagaacgt gaccgaccgt atgtacatcg cgccggagct ttcgggtggc agcgcccggc
     3961 ccggggagct gaccaaggcg gacatctact cgctggggcg gctcatcgcc tttgtctaca
     4021 ccggcgagcc gcgcgccgac ctggacgatc tgcgcatccc ctgcgccatg gtggagctgg
     4081 tgcaaaagct gaccgacccc gaccccgcgc ggcggccgga catcgacgcg gtgcgggcgc
     4141 gactggaaaa cggcggcggc cagcgccttg tctatggtgc ggcggcctgc caaggcaccc
     4201 gcccccggca ggaggatgtg ctgttggcta acggcacact gaccgcccgg ccggactaca
     4261 ccctggccga cacagccccc tgcccggctg cgttcgcagt gctggatggc atcggcggcg
     4321 ggtacggcgg agccgaggtc gcccacgcgg cggccgaagc gctggaccgc gcggctgccc
     4381 cctacctgca catggaacta gccgactaca cccagcccct ggccgcgatt tttgccgatg
     4441 ccgagcggga gacgaagcgg ttcatggccg aaaacggcta cacccgcagc ggtgcatcgg
     4501 cggcgctggt cattgccgcg tcgcgggcgg tctacaccgc aaacctcggc gacagccgtg
     4561 tctatcggta cacccacggc gcactgaccc agctgacctg cgaccatcgg tttgccctgg
     4621 gcgggcggca tgagctgtac cagtatttag gagcctcgga cgaggacaca gaaatttccc
     4681 ctaccatcgg gaaaattgac cgtgtggccg gggagcggct gctgctgtgt accgatgggg
     4741 tcagcgggaa gttgagtgat gaggaactgg ctgcaatgct caccgcccac cccgatgccg
     4801 gggatgcggc gggggcgata atagctgctg taaagtctgt tgcgacggat aatgcgacgg
     4861 ttttggttgt ggacctataa taagctaaca ccccgcagcg caaaggtaaa cagtcgtttt
     4921 cccgaaggtt gcgggccggg catacccggt ccctacatgg cggtggcgtt ttactgttgc
     4981 aataacggca cccacccccg ggagagataa atttttcccc tacacatcaa cgttgaaaaa
     5041 atggatacaa acacttttaa caatctcatc tctcaaacat ggcccgatct ccacgccgcc
     5101 tccaccctcg gcggcggcac cttcggcaca gtgtacgcct gcaccaaaac ggacgccgtc
     5161 accggcgtga cacaaaaaga ggctgtcaag gtcgtccggg tcgatttcac gcccgaggac
     5221 cgtgccaacg ccgaagagga aggcatcccc ttcgccgact actaccgcgc cgtcaaggaa
     5281 aagcgcctgc aggagatccg ctggatggtc gcgctgaaaa gcccccacat cgtccacatc
     5341 aacggctaca ccgctatcga ggaacccgat cactcggcgc tgtacatttt gatccgcatg
     5401 gactgcctga ccgcgctgga ccagctgcgc cccgctcacc tggacgacac ccccgagcag
     5461 gccgccgccc tggccgagaa ggtcgcgctg gatatctgcg acgccctgcg ggtctgccac
     5521 cagaacggcg tgctgcaccg ggatatcaag cctgccaaca tcctgtgctc cgacgccggg
     5581 gatttttatc tgggcgactt cggcatctcc aagggcaccg cccagcaggc cagcatgaca
     5641 tcgtccggca cgctggaata tgctccgcgg gaggtcatga ccgggcagta tgaccaccgc
     5701 gccgatctgt attcgctggg gctcgtgctc tacgcgctgg tcaaccactg gcgggggccg
     5761 tttctgcccg cgtaccctgc gcccatcacg ccgggcaacc gcaaccaggc ccagtacgcc
     5821 cggatgaacg gtacacctct tcccccgccc gacaactgcc ctaccgcact gtgggccgtc
     5881 attgcgcggc tctgcgcctt cgcgccggag gaacggtttg actctgccga ggccgtgctt
     5941 gcagccctgt gcgacccggg gacgcccgcg cccgcacctg ctcaacaccg ggcacacccc
     6001 aacctgcccc gcatccgcca cccgcgccgc ctggccgctg tggtattggt cttcgccgtg
     6061 gccgtgggtg ttggcgtcgg gctgtaccgt cggcaacaga tggccgcgcc ggatatctcc
     6121 ctgggggccg ggcgggacag caccgacgcc gccctggccc aggccagcga cttgttgcag
     6181 ggcaacgaaa acttcatgac cgcgcacaat ttgcagtttt gcagcgaccg caacgtcacg
     6241 ataccgggcg gcgcgttggt ctacctgaca gacccggcca aaatcgagag cgactacccc
     6301 ggctacacca ccgccgccga cgccgacgtc gaggccgggg atttgacatt aaccttcggc
     6361 gacgtgactg tcgaggacaa cgacgacggc accgtgacct acacgatcca gttgaccgag
     6421 cgggcagaca tcacccagcg ccgcacgcag gatgcgccaa tttatggcgt aggtgcgcgg
     6481 tttgcaacgg tgctgccctt cgatgccgcc actggacagg tgttccccag cctgaccgag
     6541 gactggacgc cccaccgcgc ctacacggtg cagatgaccg ccaccgtgga cggccatacc
     6601 tacgaaatgc ccatcaaccg cgacacgacc tggcaaatgg agagtgccga cggcgacgtg
     6661 gccgccacag agcaggtgga cgacctgccc ggttacggca tgccggggct gattctgcgc
     6721 gccgcctacg tgaagcagca gacgattatg attaaggccc cggctgcatc ggatttaggg
     6781 cttttggtgt gtaccgcgcc ctatgacggc tacactgaag gcgattatga aaacacgccc
     6841 ccggtgcagg cattggcgga caccccgggc tttgcggggt atgataagta ttggtatttt
     6901 gatttgaacg aattgacaaa ggaatgatcc caatgctccc tctttcctcc ctcatctccc
     6961 aaaagtggct cgatatccaa atcgacgcgc cgctcggcag cggggcgtgc ggcacggtgt
     7021 acgcctgcac caaaaaaagc gcggtcaccg gcattgaaag ccgcgaggcc gtcaaggtcg
     7081 tgcgggtgga gttttccgct gccgcccacc gccaggccga ggaggagggc atccccttcg
     7141 acgaatacta tgcccgcatc aaggcggaaa aatcgcgtga gatcgaactt cttgtcaacc
     7201 tcaaaagccc gcacatcgtc cacatcaacg agttcgacgc tgtggaagaa cccgaccaca
     7261 gcgcgtttta cctgctcatc cgcatggatc tgctgcaggc gctcacggcc ttccgggtgg
     7321 accatctgac cgacacgccg gagcaggccg gcgcctacgc ccgcaaggtc atgctggata
     7381 tctgcgacgc cctgcgcgtc tgccacgaaa acggcatctg ccaccgggat atcaagcccg
     7441 ccaacatcct gtacggcgcg accggggact tctacctcgg ggatttcggc gtgtcgcagt
     7501 acaccgccgc gccggacgct acgctcacgt cctcgggtac cgcgcggtat gccgcgcccg
     7561 agcagctgat tggccgtgcc gacccccgca gcgatttgta ctccctcggc cttgtgcttt
     7621 acgagcttac caaccactgg cgcggaccgt ttttgcccgc ctacccggcc aaactcaccg
     7681 ccgaggaccg ctacaacgcc cagtgcaagc ggctggccgg cggtgccctg cccccgcccg
     7741 acaactgccc gcccgcgctg gccgccgtca ttttgaaact gtgcgccccg gacccaggtg
     7801 accgttacca gtccgtgcag gaggtgctgg acgcgctctc ccccgccgcc gcgcctgcga
     7861 cgatgcaaaa aaagaagccc cgccgcctgt ggataggcgt ggggcttgtg gtggccgctg
     7921 tggccgtggc gtggctgacg gcgcgggtcg gtgggtggaa atactacccc aaccctggca
     7981 cggtggtgga gacgcgggac actaccgttt gggactacgc ccgcaagaac ggcatcaccg
     8041 tcagcgacgc cacgcacgtt ggttttaacg gtggggcgta taattaccca tgccagacaa
     8101 gcggtttcta tgagtatttt gaacccgacg gcgtcaccga ttggggcgca acacggctct
     8161 ccgccaccgt gaccggcgcg aaaaaaagcg accccgacgc cgacgacaat gtgacctata
     8221 ccgtcacctg cgccgcccgc gcccggatgg attacaccgt cgccgagggg gtcgagccgt
     8281 ttttgcccgg cctggacctg cgcgtattgg aactgatgga cctgcgcagc gggtacacct
     8341 tcccgctggg caatctgaac ggcccgcagg aaaccacccc gcgcagcgtc gaggtcagcg
     8401 gggactatac gttcagctac cagggacaga ccttcccggt gcaggtctcc cgcaccgagc
     8461 agtgggagta caccctgccc gactacaccg tccccggcgc ataccagacg caggtggtcg
     8521 tgtactacgc ctacaccgtc acggtgccgc agacgttgga cagcctggcg ctctacatcg
     8581 acacaacgcc ctataccggg tacagccgca cgctggacac gcgctccaaa acgctggacc
     8641 aggaggacag ctttgccgga tttgagaatt acgagttctt tgctttggac gatgtggtgt
     8701 cggattttgc
//
LOCUS       DBMDSQ010000043         8710 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_2220_length_8710_cov_1.676734, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000043 DBMDSQ010000000
VERSION     DBMDSQ010000043.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 8710)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8710)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8710
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2220_length_8710_cov_1.676734"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1388
                     /gene="asnB"
                     /locus_tag="ACZPAX_02770"
     CDS             <1..1388
                     /gene="asnB"
                     /locus_tag="ACZPAX_02770"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007048395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0070981 - L-asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HIR1112853.1"
                     /translation="AGELLFGSEIKSLLEHPGFRREVNAEALRPYLTFQYSAMNETFF
                     KGTYKLPPAHWFTYKDGRMQIERYWDADFRHPTTLSYEQCADQIDARVRESVAAHRIS
                     DVKVGSFLSGGVDSSYITACLMPDETFSVGFASPDGTHKFDETTEAGELSQKLGIKNY
                     REMLTKEQCLDAFADIQYHMDEPQSNPSSVPLYFLCQLARQHVTVVLSGEGADEIYAG
                     YEWYADTPLMQKYKHVPAPLRHLASDVSQHLPYFKGHDFIIKGSGRPEDWFIGQAHVF
                     EEKDAYDILKPKYRVGPTAREVAAPIYDRVKDLSELEKKQYLDLNLWLPGDILLKADK
                     MSMAHSLELRVPYLDREVLREAECYPDSYKLRGDTKAVLRTAANKTLPDAWANRTKKG
                     FPVPIAKWLEDPEFSAKVRKSFTSDVAAEYFDQDKLVAMLGDPKHERRKIWTAYTFLT
                     WHEQFFEKFDA"
     gene            1450..2676
                     /locus_tag="ACZPAX_02775"
     CDS             1450..2676
                     /locus_tag="ACZPAX_02775"
                     /EC_number="6.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009323955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate--amine ligase"
                     /protein_id="HIR1112854.1"
                     /translation="MSDIIPVFLGADLNCYNFARAFHEAYGVESYAYGRYPMAPTKYS
                     KIVHFTTVPDMDNEDTMLRILHDFAAQHKGKRLYLFGCTDDYAAMIIRRKAELTEYIA
                     PGPAADLYGSIQKKAEFYEVCEKFGIPYPDSKILSAPVDAAELTEDKLGFAYPIIVKP
                     SSSVDYWKHPFDTMKKVYTAATPAEAAEIVKTIYASGYPDRMVLQKMVPGGDDHMRVL
                     TAFSDENGKVRAMCLGHTMVEEHTPHGLGNHAAIVSEDTTSLPLVENIRKMLEACHYT
                     GFSNFDIKYSGTPGDYRVFEINLRQGRSNYYVTATGMNIARLVVEKWSDGGTDCVLNK
                     NEVFWHHVPAQVAFTYTEDKDLVARAKALKAQHKEACSLLYKPDLLWNPVRYACVLEQ
                     LRRQFKKFKKYYPVQK"
     gene            2848..4308
                     /locus_tag="ACZPAX_02780"
     CDS             2848..4308
                     /locus_tag="ACZPAX_02780"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HIR1112855.1"
                     /translation="MKLNDLIKHIPHTGASDMDITAIIYDSRKAAAGSLFVCLVGAWL
                     DGHTYAASAYQNGCRAFLVEHTLDLPADAVQIVTDDTRAALAVIGADFYGNPADELHL
                     IGITGTKGKTTTALLTAAIMTEAGLPCAYIGSNGVDIAGQHEATANTTPESLELHRLF
                     RKMLDAGVHHCVLEVSSQALRHHRVDGVPFEVVAFTNLSEDHIGPGEHPDFEDYKCAK
                     RRLFAEYNARTMVYNVDDPYSDFMREGFQGEQVSFGIKAEADYRGVMLAQYRNRMALG
                     IDFVCRCAGQSTHVEVMSPGAFSASDALCAIAICGAFGVTPAQASATLAHTPVQGRFE
                     VVQGLPGRTFIVDYSHNGLALTSALKTLRAYNPHRLICVFGSVGGRTQVRRKELADAS
                     SAYADYSIITSDNPDNEPPEDVIRDIAAHMAPGAPYTCITDRREAIYAAVRMAEEGDI
                     VLFAGKGHEDYQLIHGKKEHFVERDIIKEACAALKK"
     gene            complement(4350..5438)
                     /locus_tag="ACZPAX_02785"
     CDS             complement(4350..5438)
                     /locus_tag="ACZPAX_02785"
                     /inference="COORDINATES: protein motif:HMM:NF013832.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1112856.1"
                     /translation="MSILLTTVFVYALGLLLTAVGSRLAGRCTTWCCALLGLPMWVVG
                     GTVAPFCLALPQLMLAFLAAGMSVTALAVGTALAGAVANLGLALAVCLLNRRRYATAD
                     RDEFCRKCLLLLAACGVLAVFVRDGELSYTGTGLLMLLFVLFILMSIAFQHNFTYGET
                     IEAVSTSDAVPSSRLATKEYGYTAHTMAFPVMSVRNSLKNLAGVIGGLALLAGGAMAL
                     VYSATTLANLTGTIQALWAASLISFGFCLPLLAEALEHPLGSVWKRFTVRCRYYPPDS
                     LPIQLLNSAILNLTLALPISSLMYRHKLPVGAQFRSYEVPVCAAMALVLLLPPLCKHK
                     LKRWQGGVCMALYFAYLAAALFVPRAGA"
     gene            complement(5596..7035)
                     /gene="proS"
                     /locus_tag="ACZPAX_02790"
     CDS             complement(5596..7035)
                     /gene="proS"
                     /locus_tag="ACZPAX_02790"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005922033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HIR1112857.1"
                     /translation="MANDKKKMVEAITAQEVDFAQWYTDICTKAELVEYSSVKGFVVL
                     RPYGYAIWENIQKNLDARFKATGHENVAMPVLIPESLLKKEGELVNGFAPEVAWVTAG
                     GSDPLEERLAVRPTSETMFCDHFSHVLHSYRDLPMLYNQWCSVVRWEKSTRPFLRTRE
                     FWWQEGHTIHETADEAKAETEQQLNCYADFAEHDLCIPVVKGRKTDKEKFAGAEATYT
                     IEAMMKDGKALQSGTSHYFGDKFSRAYDVTFTGRDNTLQYPFQTSWGASTRLIGAIIM
                     THGDDDGLILPPAIAPIQVVIVPVAAHKPGVLDKCRELAAEIGRYARVKLDDSDKQPG
                     WKFAQWEMKGVPVRLEVGPRDIENGQCVLVRRDTREKQFVKFEELATAIPAAMDALAK
                     DLYERALQNRENRTYSATTMDEVKQIAKEHTGFIKTMWCGDLACEEAMKSEAGLSSRC
                     MPFAQEHLSDVCPVCGKPAQKMVVWGVAY"
     gene            complement(7093..7329)
                     /gene="acpP"
                     /locus_tag="ACZPAX_02795"
     CDS             complement(7093..7329)
                     /gene="acpP"
                     /locus_tag="ACZPAX_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HIR1112858.1"
                     /translation="MDSEVFSRIRGYLAEQLEVDPEKITPDSDIVNDFGADSLDVIDM
                     ITVLSDEFGVEIPDEDIENFHTVGDVVNYLEDRM"
     gene            7451..8329
                     /gene="dapF"
                     /locus_tag="ACZPAX_02800"
     CDS             7451..8329
                     /gene="dapF"
                     /locus_tag="ACZPAX_02800"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005922027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HIR1112859.1"
                     /translation="MELNFTKMQAAGNDYIYLDCRAAGLPPNAAALAVALSRRHFSVG
                     ADGVIFICPPLLADGDAAMRMFNADGSEGAMCGNGVRCVAEFLYTHGIRRDCIRIDTP
                     NAGRRTLRHVGAGLWQVEMGHFSALGADVGTTDLGGGPLLDVPLCTGGRSWRVNCVAV
                     GNPHCVVFVGDAPPEGADLALYGRELEHHPAFPGGINVEFARQISPTAFAVTVWERGS
                     GATLSCGTGACAVAASAVLTGRSGRGVPILVQMPGGTLEVRILKDDTALLLGEAAAAF
                     TGTVRIQPCKLPRGVV"
     gene            8382..>8710
                     /locus_tag="ACZPAX_02805"
     CDS             8382..>8710
                     /locus_tag="ACZPAX_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007494131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HIR1112860.1"
                     /translation="MNIHESAEDYLEKILMLQEQKGSVRSIDIAVAMGFSKPSVSVAM
                     KNLRENGYISMDADGFIKLEAPGMEIAQRIYGRHRKLTAFFVALGVDPETASKDACKV
                     EHDLSEET"
BASE COUNT         1780 a         2719 c         2596 g         1615 t
ORIGIN      
        1 aggcgggcga gctgctgttt ggcagcgaga tcaagagcct gctggagcac cccggtttcc
       61 gccgcgaggt caacgccgag gcgctgcgcc cctacctgac cttccagtac agcgccatga
      121 acgagacgtt cttcaagggc acctacaaac tgccccccgc ccactggttc acttacaaag
      181 acggccggat gcagattgag cgctactggg atgcggattt ccgccacccc accacccttt
      241 cctacgagca gtgcgccgac cagatcgacg cccgcgtgcg ggagagcgtg gccgcccacc
      301 gcatcagcga tgtcaaggtc ggttcgttcc tgtccggtgg cgtcgattcc agctatatca
      361 cggcctgcct gatgcccgac gagacgtttt ccgtcggctt tgccagcccc gacggcacgc
      421 ataagtttga cgagacgacc gaggcgggcg agctttccca aaagctcggc atcaagaact
      481 accgcgaaat gctgacgaag gagcagtgcc tcgacgcttt tgcggacatc cagtaccaca
      541 tggacgaacc ccagtccaac ccgtccagcg tgccgctcta cttcctgtgc cagctggccc
      601 gtcagcacgt cacggtcgtg ctctccggcg agggcgcgga cgaaatctac gcaggctacg
      661 agtggtacgc cgacacgccg ctgatgcaga agtacaagca cgtgcccgca ccgctgcgcc
      721 atctggccag cgacgtgagc cagcacctgc cctacttcaa gggccacgac ttcatcatca
      781 agggcagcgg acgccccgag gattggttca tcggtcaggc ccacgtcttt gaggagaagg
      841 acgcctacga tattctgaag ccgaagtacc gcgtcggccc cacggcccgc gaggtcgccg
      901 cgccgatcta cgaccgcgtg aaagatttga gcgagctgga aaagaagcag tacctcgacc
      961 tgaatctctg gctgcccggc gatattctgc tgaaggccga caaaatgagt atggcccaca
     1021 gcctggaact gcgcgtgccc tacctcgacc gcgaggtcct gcgcgaggcc gagtgctacc
     1081 ccgacagcta caagctgcgc ggcgacacaa aggccgtgct gcgcaccgcc gccaacaaga
     1141 ccctgcccga cgcctgggcc aaccgcacga aaaaaggctt cccggtgccg attgccaagt
     1201 ggctcgagga tccggagttt tccgccaagg tgcgcaaaag ctttaccagc gacgtggccg
     1261 cagagtattt cgaccaggac aaactggtgg cgatgctggg cgaccccaag cacgagcgcc
     1321 gcaagatctg gactgcctac acgttcttaa cgtggcatga gcagttcttt gagaagtttg
     1381 acgcatgaat cttatgagcc ttcccccgag ggggaagcct attttagaaa attggagatg
     1441 tactttccca tgtccgacat tatccctgta ttccttggtg cagacctgaa ctgctacaac
     1501 tttgcccgcg ccttccatga ggcctacggc gtcgagagct acgcctacgg ccgctacccg
     1561 atggccccga ccaagtattc caagatcgtg cactttacca ccgtgcccga tatggacaac
     1621 gaggacacga tgctgcgcat tttacacgat ttcgccgcgc agcataaggg caagcggctg
     1681 tatctgttcg gctgcaccga cgactatgcc gctatgatca tccgccgcaa ggccgagctg
     1741 accgagtata tcgcccccgg ccccgccgct gacctgtacg gctccatcca gaaaaaagcc
     1801 gaattttatg aagtctgcga gaagttcggc attccctacc cggattccaa aattctgtcc
     1861 gcgccggtgg acgccgctga gctgaccgag gataagctgg gcttcgccta ccccatcata
     1921 gtcaagccgt cgtccagcgt tgactactgg aagcacccct ttgacacgat gaagaaggtc
     1981 tacaccgccg ccacccccgc cgaggccgcc gagatcgtga aaaccattta cgccagcggc
     2041 tacccggacc ggatggtgct gcaaaagatg gtgccgggcg gcgatgacca tatgcgcgtg
     2101 ctgaccgcat tctctgacga gaacggcaaa gtccgcgcca tgtgcctggg ccacacgatg
     2161 gtcgaggagc atacccccca tggcctgggc aaccacgccg ccatcgtcag cgaggacacg
     2221 acctccctgc cgctggtaga gaacatccgc aagatgctgg aagcctgcca ctacaccggt
     2281 ttttccaact ttgacatcaa gtacagcggc acccccggcg actaccgcgt gtttgagatc
     2341 aacctgcgcc agggccgcag caactactat gtaaccgcca ccggcatgaa cattgcacgc
     2401 ctggtcgtgg aaaagtggag tgacggcggc accgactgcg tgctgaacaa gaacgaggtg
     2461 ttctggcacc atgtgcccgc gcaggtggct ttcacctaca ccgaggacaa ggaccttgtc
     2521 gcccgcgcca aggcgctgaa agcccagcac aaggaggcat gcagcctgct gtacaagccg
     2581 gatctgctat ggaacccggt tcgctacgcc tgcgtgctgg aacagttgag aaggcagttc
     2641 aagaagttta agaagtatta ccccgtacag aagtaaggga aaaggcttct ccctttaggg
     2701 agaagctgtc cgcgaagcgg actgatgagg ggcacgtctg ccgatgcacc tcgtaagcgg
     2761 gcggccacga taccccgccc ctcatccggc ctcgcaggct cggccacctt ccccctcagg
     2821 ggaaggcttt taagaaggat gtttttcatg aaactcaacg accttatcaa acatatccct
     2881 cacaccggcg catccgacat ggatattacc gccatcatct acgactcccg caaggcggcg
     2941 gccggttcgc tgttcgtctg cctggtgggt gcgtggctgg acggtcatac ctacgccgcc
     3001 agcgcctatc aaaacggctg ccgtgccttt ttggtggagc acacgctgga tctgcctgcc
     3061 gacgctgtgc agatcgtcac cgacgatact cgcgcggcgc tggccgtcat cggtgcagat
     3121 ttctacggca accccgccga tgagctgcac ctgattggca ttaccggcac gaagggcaag
     3181 accaccacgg ccctgctgac cgctgccatc atgaccgagg cagggctgcc ctgcgcctac
     3241 atcggttcca acggcgtgga cattgcaggc cagcacgagg ccaccgccaa cacgacgccg
     3301 gagagcctgg aactgcaccg cctgttccgc aaaatgctgg acgcgggcgt acaccactgc
     3361 gtgctggaag tcagcagtca ggccctgcgc caccaccgcg tggacggggt accctttgag
     3421 gtcgtggcct tcacgaattt gtccgaagat cacatcggcc ccggcgagca cccggatttt
     3481 gaggattaca aatgcgccaa gcgccgtctg tttgccgaat acaacgcccg cacgatggtt
     3541 tataacgtcg atgacccata cagcgatttc atgcgcgagg gtttccaggg cgagcaggtc
     3601 agcttcggca tcaaggccga ggccgactac cgcggcgtga tgctggcgca gtaccgcaac
     3661 cgtatggcgc tgggcattga tttcgtctgc cgctgcgccg ggcagagcac ccacgtcgag
     3721 gtcatgtccc ccggtgcgtt cagtgcgtca gacgcactgt gcgccatcgc catatgcggc
     3781 gcgttcggcg taacgcccgc ccaggcctct gccacgctgg cccacacccc ggtgcagggc
     3841 cgttttgagg tcgtgcaggg tctgcccggg cgcaccttca tcgtggatta cagtcacaac
     3901 ggcctggctc tgaccagcgc attaaagaca ctgcgggcct acaatccgca ccgcctgatt
     3961 tgtgtgttcg gctcggtggg cgggcgcacc caggtccgcc gcaaggaact ggccgacgca
     4021 tccagtgcct atgcggatta cagcatcatc accagcgaca acccggacaa cgagccgccg
     4081 gaggatgtca tccgcgacat tgccgcccat atggcccccg gcgcgcccta cacctgcatc
     4141 accgaccgcc gcgaggccat ctacgccgcc gtcaggatgg ccgaggaagg cgacatcgtc
     4201 ctgtttgcag gtaaaggtca cgaggattac cagctcatcc acggcaaaaa agaacatttc
     4261 gtcgagcggg atatcatcaa agaagcctgc gcggcgttga agaagtaaaa aaataccctt
     4321 gattcgtgtt atgaatcaag ggtacttttt tacgcccccg ctctcggcac aaacaatgcg
     4381 gcggctaaat aagcaaaata caatgccata cataccccgc cctgccagcg cttgagcttg
     4441 tgtttgcaca gcggcggcag aagcagcacc agcgccatgg cggcgcagac cggcacctcg
     4501 taactgcgga actgcgcacc cacgggcagc ttgtggcggt acatcaggct tgaaatcggc
     4561 agcgccagcg tcaggttcaa aatagcgctg ttcaacagct ggatgggcag cgagtccggc
     4621 gggtagtagc ggcaccgcac agtaaaccgc ttccagaccg accccagggg gtgttccagg
     4681 gcttcggcca gcagaggcag gcagaatcca aagctgatga gtgacgccgc ccacagggcc
     4741 tgtatcgtgc cggtgaggtt tgccagcgtg gtggcgctgt acaccagcgc catagctccg
     4801 cccgccagca gcgccagccc gccaataacg cccgccaggt tcttgagcga gttccgcacg
     4861 ctcatgaccg ggaatgccat cgtgtgggcc gtgtagccgt actccttcgt ggccaggcgc
     4921 gagctgggca cggcatccga ggtggacacc gcctcgatcg tttcaccata ggtaaagttg
     4981 tgctggaacg caatgctcat caaaataaac agcacaaaca gcagcatcaa caggccggtg
     5041 ccggtgtagc tcaattcgcc gtcccgcaca aacaccgcca gcaccccgca ggcggccagc
     5101 agcaacaggc atttgcggca aaactcgtcc cgatccgccg tggcataccg gcgacgattc
     5161 agcaggcaga cggccagcgc cagccccagg ttggcgacgg cgcccgccag cgcggtgccg
     5221 acggccagcg ccgtgaccga cataccggcc gccagaaatg ccagcatcag ctgcggcagc
     5281 gccaggcaga acggcgcaac cgtgccgccc acgacccaca tgggcagtcc cagcagggcg
     5341 cagcaccagg tggtacaccg tcccgccagc cggctgccca ccgccgtgag cagcagaccg
     5401 agggcgtaaa cgaatactgt tgtgagtaga atggacataa gacacccaga aagagaaaag
     5461 agaagagagg accaagaaga gagaagaaag aagcaagttt ggcaaagagc gcggcagcgc
     5521 tgtccttttc cttttcttcc ttcgttctta tctgaccaaa aaagggaacg cttcgcagcg
     5581 ttcccctttt ttgtcttagt acgctacgcc ccagacgacc atcttttggg cgggcttgcc
     5641 gcagaccggg caaacgtcac tcaaatgctc ctgggcaaag ggcatacaac ggctggacag
     5701 gccagcctcg ctcttcatgg cttcctcgca ggccaggtcg ccgcaccaca tcgtcttgat
     5761 gaagccggtg tgctccttgg cgatctgctt gacctcgtcc atcgtggtgg cgctgtaggt
     5821 gcggttctct cggttctgca gagcacgctc gtacaggtcc ttcgccaggg cgtccatggc
     5881 ggcggggatg gcggtggcca gctcctcaaa cttcacgaac tgcttctcgc gggtatcgcg
     5941 gcgaaccagc acacactggc cgttctcgat atcgcggggg ccgacttcca ggcgcacagg
     6001 cacgcccttc atctcccact gggcaaactt ccagccgggc tgcttgtcgc tgtcatccag
     6061 cttgacgcgg gcataccggc cgatctcggc ggccagctca cggcatttgt ccagcacgcc
     6121 gggcttgtgg gcggcgaccg gcacgatcac gacctggatg ggcgcgatgg ccgggggcag
     6181 gatcaggccg tcgtcgtcgc cgtgggtcat gatgatggcg ccgatcaggc gggtggacgc
     6241 gccccaggag gtctggaacg gatactgcag ggtgttgtcg cggccggtga aggtcacgtc
     6301 gtaggcgcgg ctgaacttat cgccaaagta gtggctcgtg ccgctctgca gggccttgcc
     6361 gtccttcatc atggcctcga tggtgtaggt ggcctcggca cccgcaaact tctctttatc
     6421 ggtcttgcgg cccttgacga ccgggatgca caggtcatgc tcagcaaagt cggcgtagca
     6481 gttcagctgc tgctcggtct cggccttggc ctcgtcggcg gtctcatgga tggtgtggcc
     6541 ctcctgccac cagaactcgc gggtgcgcag gaaggggcgg gtggattttt cccagcggac
     6601 gacgctgcac cactggttgt acagcatggg caggtcgcgg taggagtgca gcacatggct
     6661 gaaatggtcg cagaacatcg tctcgctggt ggggcgcacc gcaaggcgct cttccagcgg
     6721 gtcgctgccg ccggccgtga cccaagcgac ctcgggggcg aagccgttga ccagctcgcc
     6781 ctccttcttc agcagactct cggggatgag cacaggcatt gccacgttct catggccggt
     6841 ggccttaaag cgggcgtcca ggtttttctg gatgttctcc cagatggcat agccataggg
     6901 acgcagcacc acgaaaccct tgacgctgga atactcgacc aactcggcct tggtgcagat
     6961 atcggtgtac cactgggcga aatcgacctc ctgtgcggtg atcgcttcga ccattttttt
     7021 cttatcgttt gccatttcct gttaatcctc acacaaattc taaacttgtc cgcggcagca
     7081 gcgccgcaag cattacatac ggtcttccag gtaatttacc acgtcaccga cggtgtggaa
     7141 attctcgata tcctcgtcgg ggatctcaac gccgaactcg tcggacagga cggtgatcat
     7201 atcgataaca tccaggctgt ccgcgccgaa atcgttgacg atatcgctgt cgggggtgat
     7261 cttctcagga tcgacttcca gctgttctgc cagatagcca cggatgcggc tgaaaacttc
     7321 ggaatccata atttaactcc tttataaaca cagatataca tttaatatat agcgtatctg
     7381 cggctgaatg tcaagggggt aagtgttaaa ttttatgcaa gctgcatacg atgggccggg
     7441 aggtgggcat gtggaactga attttaccaa aatgcaggcc gccggcaacg attatatcta
     7501 cctggactgc cgcgccgccg ggctgccgcc aaacgccgcc gcgctggccg tggcgctgtc
     7561 ccgacggcat ttttccgtgg gggccgatgg ggtcattttt atctgtcccc cgctgctggc
     7621 ggacggcgac gccgcgatgc ggatgttcaa cgccgacggc agcgaggggg ccatgtgcgg
     7681 caacggtgta cgctgtgtag cggaattttt atacacccac ggcatccggc gggactgcat
     7741 ccgcatcgac acacccaacg ccggacggcg cacgctgcgt cacgtgggcg cggggttatg
     7801 gcaggtggag atggggcatt tctcggcgct gggggctgac gtgggcacca ccgacctggg
     7861 cggcgggccg ctgttggatg tgccgctctg cacgggcggg cgcagctggc gtgtcaactg
     7921 cgtggccgtg ggcaacccgc actgcgttgt ttttgtgggt gatgccccgc ccgagggggc
     7981 tgacctggcc ctgtatggcc gggaactgga acaccacccg gcgttccccg ggggcatcaa
     8041 cgtggagttc gcgcggcaga tcagccccac agccttcgcc gtgacagtct gggagcgcgg
     8101 cagcggggcc acgctctcct gcgggaccgg ggcctgcgcc gtggcggcct ccgctgtgct
     8161 gaccggccgc agcggacgcg gcgtgccgat tttggtgcag atgcccggcg gcacactgga
     8221 agtacgcatc ctgaaggatg ataccgcgct tttacttggt gaagccgcgg cagcatttac
     8281 tggaactgta cggattcagc cttgcaaact gccgcgcggt gtggtatgat agatgcaata
     8341 acaatttgaa caatatttca gttcggaagg gggctgcgcc catgaatatt cacgaatccg
     8401 cagaggatta tctcgaaaaa attctgatgc tgcaggaaca gaagggttct gtgcgctcga
     8461 tcgatattgc ggtggcgatg ggcttttcca agccgagcgt cagtgtcgcc atgaaaaacc
     8521 tgcgtgagaa cggctatatt tcgatggatg ccgacggttt tatcaagctg gaagcgccgg
     8581 gcatggaaat tgcccagcgc atctatggcc gccaccgcaa gctgacggcg ttcttcgtcg
     8641 cgctgggcgt tgacccggag actgcctcca aggacgcctg caaggtcgag cacgacctga
     8701 gcgaggaaac
//
LOCUS       DBMDSQ010000044         8680 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_2230_length_8680_cov_2.241753, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000044 DBMDSQ010000000
VERSION     DBMDSQ010000044.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 8680)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8680)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8680
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2230_length_8680_cov_2.241753"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..246)
                     /locus_tag="ACZPAX_02810"
     CDS             complement(<1..246)
                     /locus_tag="ACZPAX_02810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1112861.1"
                     /translation="MVNTQLKILRVFGPTGAEVSSVLRQIRDDGCPGLRLLERDGEFA
                     ICVQVSAPNRAMAEQYCDKWAARLRAKFGDDVFAEGET"
     gene            complement(485..1786)
                     /locus_tag="ACZPAX_02815"
     CDS             complement(485..1786)
                     /locus_tag="ACZPAX_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HIR1112862.1"
                     /translation="MTAEIICVGTELLLGDILNTNAQFLSRELAELGISVLHQHVIGD
                     NPARLKELVLQAKSRSNLLIFSGGLGPTEDDLTKETVAEAFGDTLAFDETEWQKILDF
                     FARTNRRPTENNRKQAMCPTRGHKLINDHGTAPGAWFEDENGHCAALMPGVPREMKAM
                     WAEQVRPILLHRQNCTIHSRTLRVLGGESAIASKVAPLFEAANPTAAIYCKTGECEIR
                     VTAREATEQAAEAACNARIAEFKEILGAAAYDVDVPALEYTVVRALREHHLHAATAES
                     CTGGMIAERLTNVPGSSEVFGFGFVTYAEAAKQKLLGVDAAVIEKYNVVSGPVAAAMA
                     FGAARKSGAELAVGITGLAGPGGALPGKPVGTVYLAGADTRTNTGYLMRLTLGGYQDR
                     AIIRTRAALYALDMLRRMALGLDVPESVRFTPETADDELDI"
     gene            complement(1783..2976)
                     /gene="thiI"
                     /locus_tag="ACZPAX_02820"
     CDS             complement(1783..2976)
                     /gene="thiI"
                     /locus_tag="ACZPAX_02820"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HIR1112863.1"
                     /translation="MKEIILAYQGEMTLKGLNRGKFEARLAKIIRWRLEPLGKFKVYQ
                     AQSTVFIEPKEDGLDMDEAFRRVSHVFGIVKLSRAVECPKDFDAICETAEAYLGETLR
                     GLRTFKVEAKRADKAYPMKSPEICRELGAYLLDKHHHLRVDVHNPQLEIMVEIRDHAA
                     YVHGPKVEAAGGLPVGTSGRALNLLSGGIDSPVAAWCMARRGLALHHIHFASPPYTSL
                     RAKLKVRDLAREIVEYTGNCTLFVVPYTKPQEYIRDHAPDVLFTVLMRRSMLRIANQV
                     AKKLELQALITGESLAQVASQTMAALACTDQAQDLPVLRPCIGMDKIEIINISRKIGT
                     FETSIEPYEDCCTIFTPPHPKTNPTLDEILAAEAAMPGLAALEAEAAENVEKIYIRMG
                     DDELL"
     gene            complement(3188..4345)
                     /locus_tag="ACZPAX_02825"
     CDS             complement(3188..4345)
                     /locus_tag="ACZPAX_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HIR1112864.1"
                     /translation="MLSDPQLHYLDNAATSRVDPAVAQAITTAMVEHWANPSSLYDPA
                     VAAQDAIMTARARVAKTLHCRSDEIYFTACGSEGNNMAVYGAARPRKHWGNKIVVTGF
                     EHPSVQRPIRALKDEGFTVVEVLPGPDGRIDTQKFLAEIDKSTVLAACMAVNNETGAV
                     QDIAALGKGIKTRNSRTHFHVDAVQAWLRMPIDLQKWREVDSLSVSGHKVHAPKGVGA
                     LFVRDSQRQNLKPPYIGGHQERGLRPGTENTPYIVGLGVAAAQGQQKLRTRAAHIAAL
                     NQQLRAGLAALDGITLNSPEDAVGEIVNFSTNCINSQTFINYLNTRAVYVSGGSACDK
                     GEPSHTLQAMGCADLTVRTALRVSFCADNTAEDVDALLDGVRDGLKELQHI"
     gene            complement(4577..5590)
                     /locus_tag="ACZPAX_02830"
     CDS             complement(4577..5590)
                     /locus_tag="ACZPAX_02830"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012343147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR1112865.1"
                     /translation="MQYVNFGQTGLKISRLGFGGIPIQRIDQPGTRELLIAAHKAGVN
                     YIDTARAYTVSEEWIGQSLEEAGLRDDFILATKCRALTKADMEAEIATSLKNLRTDHI
                     ELFQFHNPSMEGLQTILAPGGAMEALLEAKAKGVVGHIGITAHLAAVFEAALDIPEIE
                     TIMFPYNIVEQQGKELIDRCAAAGKGFIDMKPLAGGAIEDGRLALRYVLSNPAVTVAI
                     PGMATVDELNANVAGAANIDPLTPEEEAACQAVRDALGTQFCRRCNYCAPCTVGISIP
                     SVFLFQGYLNRYGLQQWGRERYATLQTKAGACIRCGACEPRCPYNLPIRQMMKKAAED
                     FGE"
     gene            5912..6718
                     /gene="proC"
                     /locus_tag="ACZPAX_02835"
     CDS             5912..6718
                     /gene="proC"
                     /locus_tag="ACZPAX_02835"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0055129 - L-proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HIR1112866.1"
                     /translation="MKYGFIGCGNMGGAVARAVCKGAGAGDVLLANRTPAKAQALAEE
                     LGCACGTNEEVASICDFIFLGVKPQMMADMLDELAPALEARAESRPFVLVTMAAGLSM
                     QQIRMMAGSGYPIIRMMPNTPVALGDGMIQYCSDDVEPDKLNEWLTAMAPAGRLDAVP
                     ESLIDAASAVSGCGPAWAYQFIEALADGGVAAGLPRAKAQEYAAQMLLGSARMVLETG
                     KHPGELKDAVCSPGGSTIQGVRLLEERAFRGAVTDAVLAAYEKTKDLGKK"
     gene            6722..7486
                     /gene="proB"
                     /locus_tag="ACZPAX_02840"
     CDS             6722..7486
                     /gene="proB"
                     /locus_tag="ACZPAX_02840"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005926257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0055129 - L-proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HIR1112867.1"
                     /translation="MRIVVKVGTSTLAHPSGLLNIRHTEDLVKVWSDIQNAGHQLIVV
                     SSAATGLGVGKLQIEKPSDIITKQAAAAVGQCELMYTYDRLFGQYNHTVAQMLLTWED
                     FDHENRLRNLQNTLERLLQLHTIPIINENDPIACEEYSLGDNDTLAALVATCIHADLV
                     VLMSDIDGLYTADPHTHPDAKLIPVVEEITPEIEMLAGGAGSSLGTGGMLTKVIAAKR
                     ATAAGVDMIITNGAHAEVLYDIVEGKPVGTRFAGHK"
     gene            7499..>8680
                     /locus_tag="ACZPAX_02845"
     CDS             7499..>8680
                     /locus_tag="ACZPAX_02845"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005932725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0055129 - L-proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HIR1112868.1"
                     /translation="MTTQEILQAAQGAKMPLALADADTKNAALEAMAVALIAASDKIL
                     EANQRDLDAARGRVSDVMLDRLALTPSRLTDMAKGMREVAALPDPVGAVLRKIVRPNG
                     LIIEKTAVPMGVIAIIYESRPNVTSDAAALAIKAGSACILRCGRDAWASCHAIVEALR
                     AGLRKARLPECAVSLLSDTSHASANELMTADGLVDLLIPRGGAGLIRACVENATVPCI
                     QTGTGICHVYVDKAADLEMAVDIVENAKTSRPSVCNAEETLLVHKDVAAQFLPMLKTR
                     LVDGRAAAGLTPVELRLDAQAAAIIDGVPAAPQDFDTEYLDYKLSVAVVDDVDAAILH
                     IARHSTHHSEAIITADAEAAARFTACVDSAAVYVNASTRFTDGGEFGLGCEMGISTQK
                     LH"
BASE COUNT         1546 a         2733 c         2783 g         1618 t
ORIGIN      
        1 cgtttcgccc tcggcaaaga catcgtcgcc gaacttggcc cgcagccgcg cggcccactt
       61 gtcacagtat tgctccgcca tggcgcggtt gggggcgctg acctgcacgc agatggcaaa
      121 ttcgccgtcg cgctccaaca ggcgcaggcc ggggcagcca tcgtcgcgga tctgccgcag
      181 tacgctggac acctccgcgc ccgtaggccc gaaaacacgc agaattttca gttgagtgtt
      241 gaccatggtg gaacctttct tgtgttgaat tgccttttcc tttggggctg cgcccgcagg
      301 cgcgtgtcgg agcgcaaccg ccgtaggcgg ctcttagcgc gtagactgaa ggtgtctgcg
      361 gccccgaccg cagacggatg aggggcagcc ttgccgttat atcccgcaaa cgggcggcga
      421 cagaaacccg cacctcatcc gccgctgcgg cggcaccttc ccctcaaggg gaaggcttgg
      481 ggttttagat atccagttcg tcatccgccg tctccggcgt aaaccgcacg ctctccggca
      541 catccaggcc cagcgccatg cggcgcagca tatccagggc gtagagcgcg gcgcgggtgc
      601 ggatgatggc gcggtcctgg tagccgccca gcgtcaggcg catcaaatac ccggtgttcg
      661 tgcgggtgtc ggcaccagcc aggtagaccg tgcccaccgg cttgccgggc agcgcaccgc
      721 ccggcccggc caggccggtg atgcccacag ccagctccgc gccggatttc cgcgcagcac
      781 caaaggccat ggcagcggcc acaggcccgg agacaacgtt gtacttttca atcacggcgg
      841 cgtccacacc cagcagcttt tgcttggcgg cctcggcgta ggtgacaaag ccgaagccga
      901 acacctcgct gctgccgggc acgttggtca gccgctcggc gatcatgccg ccggtacagc
      961 tctccgccgt ggcagcgtgg aggtgatgct cgcgcaaagc gcgtaccacc gtgtattcca
     1021 gtgcaggcac gtcaacgtca taggccgctg cgcccagaat ttctttaaac tccgcaatgc
     1081 gggcattgca ggcggcctcg gcggcctgct cggtggcctc gcgggcggtc actcgaatct
     1141 cacactcgcc agtcttgcag tagatggcgg cggtggggtt ggctgcctca aacagcggtg
     1201 cgaccttgct ggcgatggcg ctctcgccgc ccagcacccg cagcgtgcgg ctgtggatcg
     1261 tgcagttctg gcggtgcagc aaaatcgggc gcacctgctc agcccacatg gccttcatct
     1321 cacggggcac gccgggcatc agcgcggcgc agtggccgtt ctcatcctcg aaccaagcac
     1381 cgggggcggt gccgtggtcg ttgatgagct tgtggcccct ggtcgggcac atggcctgct
     1441 tgcggttgtt ctccgtcggg cggcgattcg tgcgggcgaa aaagtcaagt atcttttgcc
     1501 actctgtctc gtcaaatgcc aacgtatcgc cgaaggcctc ggccaccgtc tccttggtca
     1561 ggtcgtcctc ggtggggcca agcccgccgg aaaaaatcaa caggttgctg cggctttttg
     1621 cctgcaaaac cagctctttc agccgggcgg gattgtcgcc gatgacgtgc tggtgcagta
     1681 cgctgatgcc cagctcagcc agttcgcggc ttaaaaactg ggcattggtg ttcaggatat
     1741 cccccagcaa aagctccgta cccacgcaga taatttcagc tgtcacagca gttcgtcatc
     1801 tcccatgcgg atgtagatct tttcaacatt ctcggcggcc tcggcttcca gcgcggccag
     1861 accgggcata gcggcctcgg cggccagaat ttcgtccagt gtcgggttcg tcttggggtg
     1921 cggcggggta aagatcgtgc agcagtcctc atacggctcg atggacgtct caaacgtgcc
     1981 gatcttacgc gaaatattga tgatctcgat tttatccatg ccgatgcagg ggcgcagcac
     2041 aggcagatcc tgggcctggt cggtgcaggc cagcgcggcc atggtctggc tggcgacctg
     2101 ggccaggctc tcgccggtga tgagggcctg caattccagc tttttcgcca cctggttggc
     2161 aatgcgcagc atgctgcggc gcatcaaaac cgtgaacagc acgtcgggcg cgtggtcgcg
     2221 gatgtactcc tgaggcttgg tgtagggcac gacaaacagc gtgcagttgc cggtgtactc
     2281 gacgatttcc cgcgccagat cgcgcacttt cagcttggcg cgcaggctgg tgtacggcgg
     2341 gctggcaaag tggatgtggt gcagcgccag gccgcggcgg gccatgcacc aggcagcgac
     2401 ggggctgtca atgccgccgg acagcaggtt cagtgcgcgg ccgctggtgc ccaccggcag
     2461 accgcccgca gcctcgacct tggggccgtg gacgtaggcg gcgtggtcac gaatctcgac
     2521 catgatttcc agctgggggt tgtgcacatc cacgcgcagg tggtggtgct tgtccagcag
     2581 gtaggcaccc aactcgcggc agatttcggg gcttttcatc gggtaggcct tgtcggcgcg
     2641 cttggcctcg accttgaagg tgcgcaggcc gcgcagcgtc tcgcccaggt aggcctcggc
     2701 ggtctcgcag atggcatcaa aatccttggg gcactcgaca gcgcggctga gtttgacgat
     2761 gccgaacaca tgggacacgc ggcggaatgc ctcatccata tcaaggccgt cctcttttgg
     2821 ctcgataaaa acggtgctct gggcctggta gaccttgaac ttgcccagtg gctccagccg
     2881 ccagcggatg atttttgcca gccgcgcttc aaacttgccg cggttcaggc ccttcagcgt
     2941 catttcgccc tgataggcga ggatgatttc tttcattctg tagttccttt caaaatatgc
     3001 gactgcacgg taggggtcgg gtatgcccga ccctcggctc tacggcagac gcactgtaac
     3061 gggaaggcgg agggcgaggc atgcctcgcc cctactgtct gcggcggggt gagggcaccc
     3121 cgccctacga tttgcccgaa aaggggttcg tagggcggcc tgccctcagg ccgccgccag
     3181 cagccgttca aatatgctgc aattccttca acccatctct taccccatcc agcagtgcgt
     3241 ccacgtcctc ggcggtgttg tcggcgcaga agctcacgcg cagggcagta cgcacggtca
     3301 aatcggcgca gcccatggcc tgcagggtgt ggctcggctc gcccttatcg caggcggatc
     3361 cgcccgagac atacaccgcg cgggtgttca gatagttgat aaaagtttga ctgttgatgc
     3421 agttggtgga gaagttcacg atctccccca ccgcgtcctc gggcgagttc agcgtgatgc
     3481 cgtccagcgc ggccaggccc gcgcgcagct gttggttcag cgcagcaatg tgggcggcgc
     3541 gggtgcgcag cttttgctga ccctgggccg ccgccacgcc caagcccacg atgtagggcg
     3601 tgttctcggt gccggggcgc aggccgcgct cctggtggcc gccgatgtag ggcggcttga
     3661 ggttctggcg ctggctgtcc cgcacaaaca gcgcccccac gcccttgggc gcgtgtacct
     3721 tgtggccgga gacagacaaa ctatcgacct cacgccattt ttgcaggtca atgggcatac
     3781 gcagccacgc ctgcacggcg tccacatgga agtgggtgcg gctgttgcgg gtcttgatgc
     3841 ccttgcccaa agcggcgata tcctgcaccg cgccggtctc attgttgacg gccatgcagg
     3901 cggcaaggac ggtgcttttg tcgatctcgg ccaaaaattt ttgtgtgtcg atgcggccat
     3961 ccgggccggg cagaacctca acaacggtga agccctcgtc cttcagggcg cggatggggc
     4021 gctgcacgct ggggtgctcg aacccggtca cgacgatttt gttgccccag tgcttgcgcg
     4081 ggcgggccgc gccgtagacg gccatgttgt tgccctcgct gccgcaggcg gtgaagtaga
     4141 tttcgtcgct gcggcagtgc agggtcttgg ccacccgggc gcgggcggtc ataatggcgt
     4201 cctgcgcggc cacggcgggg tcatacaggc tgctggggtt ggcccagtgt tccaccatgg
     4261 ccgtggtgat ggcctgcgcc accgccggat ccacccggga ggtagcagcg ttgtccagat
     4321 aatgcagctg ggggtcggaa agcataacac tcattcctca cattttacac aaagttatac
     4381 aggttaaatt atacacgttc gcccccgaaa aaacaatatt ttcggggaaa aaagtgagat
     4441 aacaaaaaag cctcccctct tgaggggagg tggacgccga agacggccgg agggattccg
     4501 atgggctttg ccttgcacgc cgctacaccc gcagccgggg caggaatccc tccgcttcgt
     4561 ggaaaggcca gggcttttac tctccaaagt cctcggcggc ttttttcatc atctggcgga
     4621 tgggcaggtt ataggggcag cggggttcgc acgcgccgca ccggatgcag gccccggcct
     4681 tagtttgcag ggtggcgtag cgctcgcggc cccactgctg caagccatag cggttcagat
     4741 acccctggaa caaaaacacg ctggggatgg aaatacccac cgtgcagggg gcgcagtagt
     4801 tgcagcggcg gcagaactgc gtgcccagcg cgtcgcgcac ggcctggcag gctgcttctt
     4861 cctcgggggt cagcgggtcg atgttcgccg cgcccgccac gttggcgttc agctcgtcca
     4921 ccgtcgccat accggggatt gccaccgtca ccgccgggtt ggacagcacg taccgcagcg
     4981 ccaggcggcc atcctcgatg gccccgcccg cgaggggttt catatcgata aagcccttgc
     5041 ccgccgccgc acagcggtcg atcagctcct tgccctgctg ctcgacgatg ttatagggga
     5101 acatgatcgt ctcgatttcc gggatgtcca gcgcggcctc aaacacggcg gccaggtgcg
     5161 ccgtgatgcc gatgtgcccc actacgccct tggccttggc ttccagcagg gcttccattg
     5221 cgccgccggg ggccaggatg gtttgcaggc cctccatgct ggggttgtgg aactggaaca
     5281 gttcgatgtg gtcggtgcgc aggtttttca ggctggtggc gatctcggcc tccatgtcgg
     5341 ccttggtcag ggcgcggcat ttggtggcca ggatgaagtc atcccgcagg cccgcttctt
     5401 ccagcgattg gccgatccat tcctcgctga ccgtgtaggc gcgggcagtg tcgatgtagt
     5461 tgacgcccgc cttatgggca gcgatgagca gttcccgcgt gccgggctgg tcgatgcgct
     5521 ggatcgggat gccgccaaac cccaggcggg aaattttcag gccggtttga ccaaagttga
     5581 cgtattgcat agtgtgctcc ttttgctctg ttgcggggcg ttgaggacgc cgccccctac
     5641 aaattctccg ttacccacat tatagcaaat ctgccgcaaa aatccagacc taaagccttc
     5701 cccggagggg gaaggtggcc ccgcagggcc ggaggagggg cgcgtttggc cactattgcc
     5761 cggtcaaaag gttgctgcgg caggctctcc cctcatcaga ccgcttcgcg gccagcttct
     5821 cccccaaggg agaagccgtt tttccgttga caaaacgccc ccgctgtact accattatac
     5881 tgtatataaa ataaggtata gggggttctt tatgaaatac ggttttatcg gctgcggcaa
     5941 catgggcggg gccgtggcac gcgcggtctg caagggtgcc ggcgccgggg atgtgctgct
     6001 ggccaaccgc acgcccgcca aggcacaggc attggccgag gaactcggct gtgcctgcgg
     6061 cacaaatgag gaagtcgcca gcatctgcga tttcatcttt ctgggggtga agccccagat
     6121 gatggccgac atgctggacg agctggcccc cgcgctggaa gcccgcgccg agagccgccc
     6181 ctttgtgctg gtcacgatgg cggcggggct gtcgatgcag cagatccgca tgatggccgg
     6241 cagcggctac cccatcatcc gcatgatgcc caacaccccc gtggcgctgg gcgacggcat
     6301 gatccagtat tgctctgacg atgtggagcc ggacaagctg aacgaatggc tcaccgccat
     6361 ggcccccgcg ggccgtttgg acgccgtacc ggaaagcctg atcgacgccg ccagcgcggt
     6421 gtccggctgc ggcccggcct gggcgtacca gttcattgaa gccctggccg acggcggcgt
     6481 ggcggccggt ctgccccgcg ccaaggcgca ggagtacgcc gcccagatgc ttttgggcag
     6541 cgcccgcatg gtgctggaaa ccggcaagca ccccggtgag ctgaaagacg ccgtgtgcag
     6601 ccccggcggg tcgaccatcc agggcgtgcg cctgttggag gaacgcgcct tccgcggtgc
     6661 cgtgaccgac gccgttttgg cagcgtatga gaaaacgaag gatttgggga agaagtaaat
     6721 tatgcgtatc gtcgttaaag taggcaccag cacgctggca caccccagcg ggctgctcaa
     6781 catccgccac accgaggatt tggtcaaggt gtggagcgat atccagaacg ccgggcatca
     6841 gcttattgtc gtgtcgtcgg cggctaccgg cctgggcgtc ggcaaactgc aaatcgaaaa
     6901 gcccagtgac atcattacca agcaggccgc cgcagccgtg ggccagtgcg agctgatgta
     6961 cacctacgac cggctgttcg gccagtacaa ccacaccgtg gcgcagatgc tgctgacctg
     7021 ggaggatttc gaccacgaaa accgcctgcg caacctgcaa aacacgctgg aacgtctttt
     7081 gcagctgcac acaatcccca tcatcaacga gaacgacccc atcgcctgcg aggaatattc
     7141 cctgggtgac aatgacacac tggccgcact ggtggcaacg tgcatccacg ccgacctggt
     7201 cgtgctgatg tccgatatcg acggcctgta caccgctgac ccccacaccc accccgacgc
     7261 gaagctcatc ccggtggtgg aagaaatcac ccccgaaatt gaaatgctgg cgggcggcgc
     7321 aggttcgtcc ctgggcaccg gcggcatgct gacaaaagtc atcgctgcca agcgtgccac
     7381 cgccgcaggc gtggacatga tcatcaccaa cggcgcccac gccgaggtgc tgtacgatat
     7441 cgttgagggc aagccggtgg gcacccgttt tgcaggccac aagtaaggga ggttccccat
     7501 gaccacacag gaaattttgc aggccgccca gggcgcaaaa atgccgctgg cgctggccga
     7561 tgccgacacg aaaaacgccg cgctggaggc catggccgtg gcgctgattg ccgccagtga
     7621 taagattttg gaggcaaacc agcgcgacct ggacgctgcc cggggccgcg tgagcgacgt
     7681 aatgctggac cgcctggccc tgacccccag ccgtctgacc gacatggcaa agggcatgcg
     7741 cgaggtcgcc gccctgccgg atccggtggg cgcggtgctg cgcaaaattg tgcgccccaa
     7801 cggtcttatt attgaaaaga ctgctgtgcc gatgggcgtt atcgccatta tttacgagag
     7861 ccgccccaac gtcaccagcg acgccgccgc cctggctatc aaggcgggca gcgcctgcat
     7921 tttgcgctgc ggccgcgacg cctgggccag ctgccacgcc attgtggagg ccctgcgcgc
     7981 gggcctgcgc aaagcccgcc tgccggagtg tgcggtaagc ctgctgtcgg acacctccca
     8041 cgccagcgcc aacgagctga tgactgccga cggcctggtc gatttgctga ttccccgcgg
     8101 cggcgcgggg ctgatccgcg cctgtgttga gaacgcgacg gtgccctgta tccagaccgg
     8161 caccggcatc tgccacgtct acgtggacaa agccgctgac ctggaaatgg cggtggacat
     8221 cgtggaaaac gccaagacca gccgccccag cgtctgtaac gccgaggaaa cgctgctggt
     8281 tcacaaggac gttgcagcgc agtttttgcc gatgttaaag acccgcctgg tggacggccg
     8341 cgccgccgcc ggcttaacgc cggtggagct gcgcctggac gcccaggccg ccgccatcat
     8401 cgacggtgtg cctgccgcgc cgcaggattt tgacacggaa tatctggact acaagctgag
     8461 cgtagccgtg gtggacgacg tggacgcagc gattttgcac attgccaggc attccaccca
     8521 ccacagcgag gccatcatca cagcggacgc cgaggcagcg gcgcggttca cagcctgtgt
     8581 ggacagcgca gcggtctacg tgaacgccag cacccgcttt accgacggcg gcgagtttgg
     8641 tctgggctgc gaaatgggca tcagcaccca aaagctgcac
//
LOCUS       DBMDSQ010000045         8529 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_2261_length_8529_cov_1.897841, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000045 DBMDSQ010000000
VERSION     DBMDSQ010000045.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 8529)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8529)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8529
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2261_length_8529_cov_1.897841"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..627
                     /locus_tag="ACZPAX_02850"
     CDS             <1..627
                     /locus_tag="ACZPAX_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016322606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HIR1112869.1"
                     /translation="EEEGAFTSRAQLKKVKGLGPKAYEQCAGFLRLPEAKNRLDATAV
                     HPESYPAAKALLDACGYTTAEIGSDKLAGLPAAVKAKGTKNLCETLGIGEPTLNDIVA
                     ELCKPGRDVRDSLPKPLLRSDVMGLDDLKPGMELTGTVRNVIDFGAFVDLGVHQDGLV
                     HVSQISDKFIKHPREVLKVGDIVRVKVLSVDTGKKRIALTMKGVPQQN"
     gene            1046..1267
                     /locus_tag="ACZPAX_02855"
     CDS             1046..1267
                     /locus_tag="ACZPAX_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007045754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HIR1112870.1"
                     /translation="MVKQVKVSNFTEVKGIVSAAAKCYNDVGVHDMKGSIADAKSILG
                     MMSLDYSNPVKIVCEDEHDLNRVVSALKQ"
     gene            1641..2072
                     /gene="rplM"
                     /locus_tag="ACZPAX_02860"
     CDS             1641..2072
                     /gene="rplM"
                     /locus_tag="ACZPAX_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019680187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HIR1112871.1"
                     /translation="MSTTLAKPAEMADRKWYVIDAAGKPLGRVAAKAAVILRGKNKTT
                     FTPNVDCGDHVIIINCDEAVLTGKKLEKKFHRTHSGWIGGLKETQYKTLMAENSDKAM
                     TYAVKGMIPSNTIGAKAMTRLHCYKAADHAHAAQKPEAVEI"
     gene            2091..2489
                     /gene="rpsI"
                     /locus_tag="ACZPAX_02865"
     CDS             2091..2489
                     /gene="rpsI"
                     /locus_tag="ACZPAX_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006354195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HIR1112872.1"
                     /translation="MYETKPYFYGTGRRKHSVARVRVYNGTGKMTINGRDINDYFGLE
                     TLKLLANSPLVLTENEGKFDVVVNVVGGGCAGQAGAIRHGLSRALLQFNPELRATLKK
                     AGFLTRDPRMKERKKYGLKAARRAPQFSKR"
     gene            2582..3712
                     /locus_tag="ACZPAX_02870"
     CDS             2582..3712
                     /locus_tag="ACZPAX_02870"
                     /inference="COORDINATES: protein motif:HMM:NF019142.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D domain-containing protein"
                     /protein_id="HIR1112873.1"
                     /translation="MALSVKTKEHIRRWAARVLPKWGALACGGAALAAAIAFTGTALH
                     FTTVNDTHGGSVRILTASTDLDTVLEQADTPQLREHDRAVWTHTDDGEQLDVLRAYTV
                     PITADGTTQEIITTGATTAELLAQAGLAWTEDDILSSGPDEITPEGGSITLQRVSYVE
                     YTQDEVIPAAVEDIPTSLFYRKQDKTMTLQEGVDGLDTVTYRETWIDGQWTATDEIDR
                     VTQAGMVPTMEKVYGEGAPVSQFVGPEIVDGVPAEGVAEAYTNQRSTGYSASETAKGA
                     SGRRLTYGTVAINPNIIPYGSLMYITSADGKFVYGYAYAADTGTAMMAGSAFIDLYYE
                     TYDESVMSAVIPVNVYVLDDETAAKYKEQNDAILAADTVVGR"
     gene            complement(3778..4407)
                     /gene="upp"
                     /locus_tag="ACZPAX_02875"
     CDS             complement(3778..4407)
                     /gene="upp"
                     /locus_tag="ACZPAX_02875"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013726447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HIR1112874.1"
                     /translation="MSVVEIVDHPLIQHKISLLRDRNTGTKEFRDLVSEIAMLLCYEA
                     TRDLPTEEVEVETPVALARTKVLAGRKLALVPILRAGLGMVDGMLNLIPAAKVGHIGM
                     YRNEETLEPVEYYCKLPNDINEREVFVLDPMLATGGSACDAIGQIKKRGAKHIKFIGL
                     VAAPQGLKALYEAHPDVDIYVGALDEKLNDNGYIVPGLGDAGDRIFGTK"
     gene            complement(4463..4915)
                     /gene="rpiB"
                     /locus_tag="ACZPAX_02880"
     CDS             complement(4463..4915)
                     /gene="rpiB"
                     /locus_tag="ACZPAX_02880"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007045960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HIR1112875.1"
                     /translation="MKFDKPIALAADHGGYELKEAIKAHLNELGIAYTDFGTDSTASV
                     DYPDYAAKGCKAVQDGSCALVILCCGTGVGMSMCANKMQGIRACCCSDTFSAEFTRRH
                     NNANALCLGGRVVGAGLACQIVDAFLNAAFEGGRHQQRIDKMMALENH"
     gene            complement(4905..5615)
                     /gene="tsaB"
                     /locus_tag="ACZPAX_02885"
     CDS             complement(4905..5615)
                     /gene="tsaB"
                     /locus_tag="ACZPAX_02885"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015565511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HIR1112876.1"
                     /translation="MNLLALDTAGKTAAVAVLRDDTLLYEAQCNNGLTHSETLLPMID
                     TALKACGLTVDDLDVLGVTNGPGSFTGLRIGLAVVKGLALPKQIPCAGVSTMAALAYG
                     LSGEGTVIGAQDARRGQVYWGAFGLASHDRLTPDAAEPVTALENFVQSCKKPLFFVGD
                     GATLCYNTYKSVPGVAAVPQPLQVLRGVGVALAAKAMWEKGECVPPAALLPDYHRLSQ
                     AERERTEREKTEGKQNEV"
     gene            complement(5632..6045)
                     /gene="tsaE"
                     /locus_tag="ACZPAX_02890"
     CDS             complement(5632..6045)
                     /gene="tsaE"
                     /locus_tag="ACZPAX_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015536877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HIR1112877.1"
                     /translation="MQEFTTHSREETVAAGRDFAKTLPSGALIAFTGGLGAGKTAFCQ
                     GLAEGLGCTDPVSSPTFAIVNYYRGPRPFAHFDLYRIHTENDLAAAGFYDYLDMGAVV
                     ACEWSENCTELLAPENPIKIHIERVDETTRKITIE"
     gene            6200..7363
                     /locus_tag="ACZPAX_02895"
     CDS             6200..7363
                     /locus_tag="ACZPAX_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007045957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0015648 - lipid-linked peptidoglycan
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HIR1112878.1"
                     /translation="MYPLIRRYLRRVDVFYLLLCAACSALSVVVLVSIGQTQLGSNNK
                     ASVQLIASLLGLMLAVVFSTADYHALARAWPLHAVVAWGLVLPTLFLRNVNTGLVTIG
                     YDAGGTSNYSWYRVGGMTFQPAELAKISFILTLALHLSNVRGQVNKPKNLLLLLLHIG
                     LPAAAIHVQGDDGTALVFLGIGLVMLFAGGLSHWLVAGGLAAGAVGGAALLVLRPGIL
                     KGYQFKRILAVLTPDDPALADITYQQNKGAMAIGTGGLTGQGLFSGEHIFVPNAWNDF
                     IFAYLANVLGFIGAAVVLVLLLALCLRTLQTGLRCPDALGCNICVGIFAALFLQCVIN
                     LGMNLQVLPVIGVTLPFFSAGGSSVVMMYLCVGIVLSVGMNTRRSKLDMQRII"
     gene            complement(7401..7610)
                     /locus_tag="ACZPAX_02900"
     CDS             complement(7401..7610)
                     /locus_tag="ACZPAX_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007045956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HIR1112879.1"
                     /translation="MSCFKKLLLLLAIIGGINWGIYGIWGFNAVGWLLGGSMSWLSRT
                     VFIIVGASAVCLIPGLFMPNPKTAE"
BASE COUNT         1814 a         2478 c         2482 g         1755 t
ORIGIN      
        1 gaggaggagg gggctttcac ctcccgtgcc cagctgaaaa aggtcaaggg cctcggcccc
       61 aaagcctatg agcagtgcgc gggcttcctg cgtctgcccg aagccaagaa ccgcctggac
      121 gccactgctg tccaccccga aagctacccc gccgccaagg ccctgctgga tgcctgcggc
      181 tacaccactg ccgagatcgg cagcgacaaa ctggcgggtt tgcctgccgc cgtcaaggct
      241 aagggcacca agaacctgtg tgagacgctg ggcattggcg agccgacgct gaacgacatc
      301 gtggccgagc tgtgtaagcc gggccgcgac gtgcgcgaca gcctgcccaa gccgctgctg
      361 cgcagtgacg ttatggggct ggacgacctc aaacccggca tggagctgac tggcaccgtg
      421 cgcaatgtca ttgatttcgg cgcattcgta gacctgggtg tccaccagga cggactggtc
      481 catgtttccc agatcagcga taaattcatc aagcacccgc gggaagtgct gaaggtcggc
      541 gatatcgtgc gggtcaaggt gctcagtgtc gataccggca aaaagcgcat tgccctgaca
      601 atgaagggcg taccccagca aaactgatac aaaagcgaca ggaacatata caaatgttgc
      661 acgcggattt acatggtgtt tgatttttaa caaggaatgt tgtgcaacta aagcagaaat
      721 caacaaaaac gttcagaaaa cgtaaaaatt cgcttgtaaa aacataatac ctgtgaacaa
      781 acgcacaggg cacagcccaa aagctgtcat tcttaccaaa agccaaaaat gaaggatttt
      841 ctcttttgtg caggaaatgg aaaaaatcac ctttgtgcat tatgaacaaa cacaaggcac
      901 ataattgtgc tataatgcac aacgtaggga gcagatagag catggtggct cacctccctg
      961 ccgcaggccg tgcgcccggt tccatcctgg ataccggtgc tgaagacgcg gcagaatgcg
     1021 tgggaaaaat cggaggttct ttattatggt gaaacaagtc aaggtttcca attttactga
     1081 ggtcaaggga atcgtgtctg ctgccgccaa gtgttataac gatgtcggcg tgcatgacat
     1141 gaagggcagc attgccgatg caaagagcat cctgggcatg atgagcctgg attacagcaa
     1201 tcccgtcaag atcgtctgcg aggatgagca tgacctgaac cgcgttgtca gcgcgctcaa
     1261 gcagtaaaat aaaccaagcc cgccctgtgg tgtatgccgc agggcggttt tttatttgca
     1321 gataaagata ttgctgaggt gtaggggccg cctatgtgca gcccgcggct ttcccgcaag
     1381 atgtcgtctg ccctgcatac ccggccccta cggccctgcg taaattttta taaaaataga
     1441 gtttgtgaaa acgcgagaaa acaagagcat ttacgagaat taaagagcta tcaaagaata
     1501 aattaaaatt ctttccaaaa accgttgact atggccccgc tttctgctat aatcacactt
     1561 gcgcgaaaaa gcgccctgtg cggcgctgtt aaataccgtt ataagacgga cgtgttaatt
     1621 tctcaatgga ggttcctact atgagcacga ctctcgctaa gcccgctgaa atggctgacc
     1681 gcaagtggta tgtgatcgac gccgccggca agcccctcgg ccgcgttgct gccaaggctg
     1741 ctgtcatcct gcgcggcaag aacaagacca ctttcacccc caacgttgac tgcggtgacc
     1801 acgtcatcat catcaactgc gacgaggctg tcctgaccgg caagaagctg gagaagaagt
     1861 tccatcgcac tcattccggc tggatcggcg gcctgaagga gacccagtac aagaccctga
     1921 tggctgagaa cagcgacaag gccatgacct acgctgtcaa gggcatgatc ccctctaaca
     1981 ccatcggcgc caaggctatg acccgcctgc actgctacaa ggctgctgac catgcccatg
     2041 ctgctcagaa gcccgaagct gttgagattt gagtgaggag gcaatagaag atgtacgaaa
     2101 ccaaacctta tttctacggc actggccgtc gtaagcattc cgttgcccgc gttcgcgttt
     2161 acaacggcac cggcaaaatg accatcaatg gccgtgacat taacgactac tttggtctgg
     2221 agaccctgaa gctgctggcc aacagccccc tggttctgac tgagaacgag ggcaagttcg
     2281 acgtcgtcgt caacgtcgtc ggcggtggct gcgccggtca ggctggcgct atccgccacg
     2341 gcctgtcccg cgccctgctg cagttcaacc ccgagctgcg cgctaccctg aagaaggctg
     2401 gcttcctgac ccgcgatcct cgtatgaagg aacgtaagaa gtacggcctc aaagccgctc
     2461 gtcgcgcacc gcagttcagc aagcgctgat tacccgctgc gcagcgaaag ccttatccca
     2521 cagcaaaact ggcgctggtc cttgtgggcc ggcgcctttt ctatagcaaa aaggagaacc
     2581 catggcctta tccgtaaaaa cgaaagagca tatccgccgc tgggcggcgc gggtgctgcc
     2641 caagtggggc gcactggcgt gcgggggcgc cgcattggcg gcggccatcg cgtttaccgg
     2701 cacggcgctg cactttacca ccgtcaacga cacccacggc ggcagcgtgc gcatcctgac
     2761 ggcatccacc gacttggaca ccgtgctgga gcaggccgac accccgcagc tgcgggagca
     2821 tgaccgcgcc gtctggacgc acaccgacga cggtgaacag ctcgacgttc tgcgcgctta
     2881 caccgtgccc atcacggcgg atggcaccac gcaggagatc atcaccaccg gggcaacgac
     2941 ggcggaattg ctggcccagg ccgggctggc ctggaccgag gacgatatcc tgagcagcgg
     3001 ccccgacgag atcacccccg agggcggcag tatcacactg cagcgggtca gctatgtgga
     3061 gtacacccag gacgaggtca tccccgctgc ggtggaggat atccccacca gcctgttcta
     3121 ccgcaagcag gacaagacga tgaccctgca ggagggcgtg gacggcctgg acaccgtgac
     3181 ctaccgcgag acctggatcg acggccagtg gacagccacc gatgaaatcg accgtgtgac
     3241 ccaggctggc atggtgccca ccatggagaa ggtctacggc gagggcgcgc ctgtgtcgca
     3301 gttcgttggg ccggagatcg tggacggcgt ccccgctgaa ggtgtggctg aggcctacac
     3361 gaaccagcgt tccaccggct actcggcatc ggaaacggcc aaaggtgcca gcggccgccg
     3421 cctgacctac ggtacggtgg ccatcaatcc caacatcatt ccttacggct cgctgatgta
     3481 cattaccagc gccgacggca agttcgtcta cggctatgcc tacgccgccg acaccggcac
     3541 ggccatgatg gcaggctcgg ccttcatcga tctgtactac gagacctatg acgagtccgt
     3601 gatgagtgcg gtcatcccgg tgaacgtcta cgtgctggac gacgagaccg ccgccaagta
     3661 caaagagcag aacgatgcga tcctcgcggc tgacacggtg gtcggcagat aaagcagcag
     3721 acaaaaaata acagggtcgt gaaccgctgc ggttcacgac cctgttattg tacaatttta
     3781 cttcgtgccg aaaatacggt cgccggcgtc gcccaggccg gggacgatgt agccgttgtc
     3841 gttcagcttc tcgtccaggg caccgacata gatgtctaca tcggggtggg cctcatacag
     3901 ggccttcagg ccctggggcg cggccaccag gccgatgaac ttaatgtgct tggcaccgcg
     3961 ctttttgatc tggccgatgg cgtcacaggc actgccgccg gtggccagca tcgggtccag
     4021 aacaaagacc tcgcgctcgt tgatgtcgtt gggcagcttg cagtagtact cgaccggctc
     4081 cagcgtctcc tcgttgcggt acataccgat gtggccgacc ttggcggcgg ggatcaggtt
     4141 cagcataccg tccaccatgc ccaggcctgc gcgcaggatg ggcaccaggg ccagcttacg
     4201 gccggccagg accttggtgc gggccagggc aacgggggtc tcgacctcga cttcctcggt
     4261 gggcaggtcg cgggtggcct catagcagag cagcatcgcg atctcgctta ccaggtcacg
     4321 gaactccttg gtgccggtgt tgcggtcgcg cagcaggctg attttgtgct ggatcagggg
     4381 atggtcaaca atttcaacaa cgctcatggt atgatctcct tgcaattcaa aaatattcag
     4441 cctgccgtgc aggcaacgaa acttagtgat tttccagtgc catcatcttg tcgatgcgct
     4501 gctggtggcg gccgccctcg aacgcagcgt tcaggaaagc gtccacgatc tggcaggcca
     4561 gtcccgcacc caccacgcgg ccgcccaggc acagggcgtt ggcgttgttg tggcggcggg
     4621 tgaactccgc gctgaaggtg tcgctgcagc agcaggcacg gatgccctgc atcttgttgg
     4681 cgcacatcga catacccacg ccggtgccgc agcacaggat gaccagcgca cagctgccgt
     4741 cctgtacggc cttgcagccc ttggcggcat agtcggggta gtccacgctg gcggtggagt
     4801 cggtgccaaa gtcggtgtag gcaatgccca gctcgttcag gtgtgccttg atggcctctt
     4861 tcagctcata gccgccgtgg tcggcagcca gtgcgatcgg tttgtcaaac ttcattttgt
     4921 ttcccctctg ttttttcacg ttccgtgcgc tcccgctcgg cctggctcag ccggtggtaa
     4981 tccggcagca gggcggcggg gggcacacat tcgccctttt cccacatggc cttggcggcc
     5041 agcgctacgc ccacaccgcg cagaacctgc agcggctgtg gcacagcggc cacgcccgga
     5101 acgctcttat atgtattgta acacaaagtt gcgccgtcgc caacaaaaaa caaaggtttt
     5161 ttacagcttt gtacaaaatt ttccagcgct gtgaccggct cggcggcgtc tggggtcagg
     5221 cggtcatgac tggcaaggcc aaacgcgccc caatacacct gcccgcggcg ggcgtcctgt
     5281 gcgccgatga cggtgccctc gcccgaaagg ccgtaggcca gtgcagccat cgtggagacg
     5341 cccgcgcagg ggatctgttt gggcagggcc agccccttga cgacggccag cccgatgcgc
     5401 agcccggtaa agctgccggg gccgttggtc acacccagca cgtccaaatc gtccacggtc
     5461 aggccgcagg ctttcagcgc ggtatcgatc atgggcagca gtgtctcact gtgggtcagg
     5521 ccgttgttgc actgggcctc gtacagcagg gtatcgtccc gcagcaccgc cacagccgcc
     5581 gttttgccgg cggtgtccaa agccaataaa ttcattgaaa tcacctattt atcattcaat
     5641 cgtaatcttc cgcgtcgtct catccacacg ctcaatgtgg attttgatgg gattttccgg
     5701 cgccagcagc tccgtgcagt tctcgctcca ctcgcaggca acgacggctc ccatgtcgag
     5761 gtagtcatag aacccggcgg cggccaggtc gttttcggtg tggatgcggt acaagtcaaa
     5821 atgtgcgaac ggccgcgggc cgcggtagta gttgacaatg gcaaatgttg ggctggatac
     5881 cgggtcggtg cagcccaagc cctcggccag gccctggcag aaggcggtct tgcccgcgcc
     5941 caggcccccg gtgaacgcga tcagcgcccc cgacggcagc gtcttggcaa aatcccgccc
     6001 ggcggcgacg gtttcttccc gtgaatgggt cgtaaattcc tgcataggca aactccctgc
     6061 ctgcggccga atagaacagt ggccgcactc gtatatatta tagtacacaa aaaccgattt
     6121 gtaaaattta atctgtaact tggcaaaaac cgtcgaaatg cggtataata caaatactat
     6181 caagggaagg agtccgccca tgtatccgct tatccgccgg tatctgcgcc gggtcgatgt
     6241 gttctatctg ctgctctgcg cggcgtgctc ggcgctcagc gtcgtggtgc ttgtgtccat
     6301 cggccagacc cagctgggat ccaacaacaa ggcgtcggtg cagcttatcg ccagcctgct
     6361 ggggctgatg ctggcggtgg tgttctccac tgcggattac cacgcgctgg cccgggcctg
     6421 gccgctgcac gccgtggtgg cctgggggct ggtgctgccc accctgtttt tgcgcaacgt
     6481 caatacgggg cttgtcacaa tcgggtacga cgcgggcggc acctccaatt acagctggta
     6541 ccgcgtcggc ggcatgacat tccaacccgc cgagctggca aaaatcagct ttattttgac
     6601 attggcgctg catttgagca acgtgcgcgg ccaggtcaat aagccgaaga atttgctgct
     6661 gctcctgctg cacatcgggc tgcctgcggc ggccattcat gtgcagggcg acgacggcac
     6721 ggcgctggtg tttttaggga taggcctcgt catgctcttt gcgggcgggc tgtcccactg
     6781 gctggtggcg ggcggcttgg cggcgggggc tgtgggcggc gcggcgctgc tggtgctgcg
     6841 gcccggcatt ttaaaaggat accagttcaa gcgcattctg gccgtgctta cgcccgacga
     6901 ccccgcgctg gcggacatca cctaccagca gaacaagggt gccatggcca tcggaaccgg
     6961 cgggctgact gggcaggggc tgttttccgg tgaacacatc tttgtgccca acgcctggaa
     7021 cgattttatc tttgcgtacc tcgccaacgt gctgggcttt atcggtgcgg cggtggtgct
     7081 ggtgctgctg ctggcgctct gcctgcgcac gctgcaaacc ggtctgcgct gccctgacgc
     7141 gctggggtgc aatatctgcg tgggcatttt tgcggcgctg tttttgcagt gtgtcatcaa
     7201 cctgggcatg aatttgcagg tgctgccggt catcggcgtg acgctgccgt tcttctcggc
     7261 gggcgggtcc agcgtcgtga tgatgtatct ctgcgtcggc attgtgctga gcgtcggcat
     7321 gaacacccgc cgcagcaagc tggatatgca gcggatcata taagtaaaac gggagcggcg
     7381 gaaagctgct cccgttttta tcattctgcg gttttggggt ttggcatgaa aagcccgggg
     7441 atcagacaga ccgccgacgc accgacgatg atgaacacgg tgcgggacag ccagctcatg
     7501 ctgccgccca gcagccaccc cacggcgtta aagccccaga tgccgtagat gccccagttg
     7561 atgccgccga taatggccag caaaagtaaa agttttttga aacaagacat ggatgatacc
     7621 ctcctttgtg ggagagtatg tgcggggatg tgggagttta tgcgcggctg aagcgggacc
     7681 cgcgacttac cgccgcatcc ctttcacggt tcatccgcag ggaaaaatcc tgcccccctt
     7741 cgcggggcgt cgaggacgcc gccccctaca ttccttgtcg taacccttcc gcctatttgc
     7801 aggggccgca tataggcggc cgccaacctt aacgccatat ccggcttacg catcgtcacc
     7861 ctgtaggggc cgggcatgcc cggcccgcag ttttgccata agtacccgct tagcattaca
     7921 aactggaatt tgttattctc ctatggaggc atcgctatcg tctttaaggc cacaaatcgc
     7981 aactatacca ttatttttat aacttacaac gagagtagtg tcattcccaa gtttccaaca
     8041 tgcagtgctg ttttcactgg tatcaggctt cccccatacc tctctaagct ggtcaatatg
     8101 gtaccctggc aaaaggttat taacatcggc ttcactcatt tctgcgcaac gccagcgagg
     8161 gtcacggcat tatcaagatg ggcaacagtg tattaccatt caccaatctg gagcccaagg
     8221 atagtgatgt ctatcagctc atcacaacga aaccgggaga aaggaagaag caagaatgat
     8281 tttgcataaa gaaaaggcgg gccggagccc gccttagagt cagtggcagt ttagtactgc
     8341 cagatattgt tttctaaatc atgcagacgc gctttgtagt cgttaataag cgcctgcgtg
     8401 ttttctgttt ataaatgcta gttctagaac agtccataag ccttgagaac tcagatacat
     8461 taatctgttt gttttgatat tggttgtagt atttccaaaa tttgtccggt aagctgtcag
     8521 atgtagtct
//
LOCUS       DBMDSQ010000046         8389 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_2308_length_8389_cov_1.692085, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000046 DBMDSQ010000000
VERSION     DBMDSQ010000046.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 8389)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8389)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8389
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2308_length_8389_cov_1.692085"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(99..1406)
                     /gene="rsmB"
                     /locus_tag="ACZPAX_02905"
     CDS             complement(99..1406)
                     /gene="rsmB"
                     /locus_tag="ACZPAX_02905"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005926000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HIR1112880.1"
                     /translation="MTPRRLAVKALMHQEQAGYANLVLDAELKKCAPPLDGRDAAFAA
                     RIFYTTLERLPLLDYILNQFTKKPVNKLDAPVRAVLRAGLAQARYMNVPLPAAVNESV
                     KLTKALGKASAAGMVNAVLRRAASAKVCESEFADPLDRLATYYCLSRPVAQLFYEQFG
                     EEAFPLAAAFYDKPKTTIRVNTLRTNDAALTALLQSEGHTVKPGPWAGALVIEFAGSP
                     AASAAFKEGLFHVQGLASQFAALCVDAKPGQQVLDLCAAPGGKSLTLAEAMQDKGQLV
                     SGEFVPTRVPLLQRSFDRCGVTCAVAVENDATRHNPGWPKFDRVLCDVPCSGLGVIAK
                     KPDIRYKDLDGIENLLATQQKILQNGTDSLAENGRLVYSTCTVNYKENQAQVEKFLSA
                     NPDFHVVKPETALPGADIGPLGTLFLPHKTGTDGFFVAILERK"
     gene            complement(1403..2323)
                     /gene="fmt"
                     /locus_tag="ACZPAX_02910"
     CDS             complement(1403..2323)
                     /gene="fmt"
                     /locus_tag="ACZPAX_02910"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005926002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HIR1112881.1"
                     /translation="MRILFMGTPDIAAESLAALLGAGHEVCAVFTRRDKPVGRKQILT
                     APPVKQLAMERGIPVYQPRTLRDGSSDDVIKELAPDIIVVVAYGCIIPPQLLHAAKYG
                     CINLHVSLLPKYRGSAPIQWSVLNGNAGTGVTIMQLDEGLDTGDILMVEPVAIDPEET
                     SGELFDRVSAVGAKTLVTALEKIKAGELTPQKQDDAQATMAPPLTKDMARFDFTQDAA
                     HIHNWVRGMNPWPVAWFEHDGKRIKVLECRLAENAQNATPGTVLALKPLTIAAANGAV
                     ALLTVTPEGGKPMAGTAWAAGRRLKAGDSL"
     gene            complement(2329..2844)
                     /gene="def"
                     /locus_tag="ACZPAX_02915"
     CDS             complement(2329..2844)
                     /gene="def"
                     /locus_tag="ACZPAX_02915"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HIR1112882.1"
                     /translation="MALRTIVQDGDPILKKVCRPVTKFDDRLGTLLDDMKETLLAANG
                     LGLAGPQVGMMRRLFICLDDRDLPEEVPENYEYKFIEFINPEILELSDEQVELYEGCL
                     SFPGHNGAIKRSKHVKVKAQDRHGEWFEMEADDMLARCIQHENNHLDGITIMDLAEHF
                     YEDDYPEENED"
     gene            complement(2863..5130)
                     /gene="priA"
                     /locus_tag="ACZPAX_02920"
     CDS             complement(2863..5130)
                     /gene="priA"
                     /locus_tag="ACZPAX_02920"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication restart helicase PriA"
                     /protein_id="HIR1112883.1"
                     /translation="MTLTLAQAAVNDANIHFDRLYSYTIPTQLVGRVFPGSMVLVPFG
                     RGDKARMAVVLAVEEVDEADAPKRLKTLYDAAPEDARLTPDLLELVRFLKERTFCTWF
                     EAVKAVIPYGAQYRPAVVDGKHVMQSRLRRSTERLYTLAGELPEKPKPGPRQLAAVEA
                     LKSGPLTARQLDEVGISKATLDGLVKKGVLTAAEQDKSLDLFAAIPFDPQPLELSPEQ
                     QQVFDNLLPNLADFKPHAALLHGVTGSGKTIVFLKLIQRTLELGRRALVLVPEISLTP
                     QMIRRLKSTFGSRLAVQHSALNNTERLLQWRMIQQGNADIVVGTRSAVFAPLQNLGLI
                     IMDEEQEHTYQSESAPRYDAHDVAKKRAVMENALLLFASATPLTETYHAAESGKLQLV
                     QLTHRYGGRPLPSVNFIDMRAELAAGNPREVSVRLARELRENIDNGEQSILLLNRRGY
                     RTIGMCATCGHVLKCPNCSVPLVYHKPQQALMCHHCGHTMQPLPQTCPECGGKINYSG
                     FGTQRVEEELAELLPDARILRMDQDSTARKDAHETMLAQFARQEYDILLGTQMVAKGL
                     DFEKVTLVGVLGIDSLLFGQGFRAYESVFSLITQVVGRGGRAELPGRALIQTCVPDHP
                     VLQLAAAQDYEGFYREEITFRRFGLYPPFCSFVVVGFVGDQEGAVFIAAQRFGALLAQ
                     HAAQKPNIPLRVLGPAPMNITMLNNKYRYKLTLKCRNDAAFRGLLHETLDAYDAEKLP
                     QKASVVLDFNSDGDL"
     gene            complement(5127..5705)
                     /gene="gmk"
                     /locus_tag="ACZPAX_02925"
     CDS             complement(5127..5705)
                     /gene="gmk"
                     /locus_tag="ACZPAX_02925"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - GMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HIR1112884.1"
                     /translation="MKGEKYLFVVSGPSGTGKDTVVAALLKKHPEIQHTVSATTRAPR
                     EGEKDGINYHFMSVADFEDHLAHDQIVEHTKYCENYYGTLRSEIEGRMKLGIPVILVI
                     EVEGAGNIKKIYPGATTIFVLPPDMQELERRLRCRGTEDEETIQRRLKRAEAEIANSV
                     NYDEHVVNVEVDSCAESLYSIIQFKLQHGSDE"
     gene            complement(5708..5962)
                     /locus_tag="ACZPAX_02930"
     CDS             complement(5708..5962)
                     /locus_tag="ACZPAX_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014118186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF370 domain-containing protein"
                     /protein_id="HIR1112885.1"
                     /translation="MKLLNIGFGNMVNADRIIGIVSPDSAPIKRLVQDAREKGALIDA
                     SYGRRTQAVLIMDSDHVILSGIPLESITGRAAEETEEKRK"
     gene            complement(5978..6856)
                     /locus_tag="ACZPAX_02935"
     CDS             complement(5978..6856)
                     /locus_tag="ACZPAX_02935"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015538477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HIR1112886.1"
                     /translation="MVLSMTGYGRAGALLHGRDIKVELRSVNARFFEYSSRLPRSCAF
                     LEDKLKKLVAAKVSRGKVELSLSIQTVTAADTVVTVNWQLAEGYRAALNAMAERMDLK
                     NDVSVGMLSRFPDVLTQTAAPTNEDELWEDVKSVAEQAVDAFISMRAVEGEKMKADVA
                     SRLTTIETLVGQIEENSAGRVQAYSDKLYARLQELLGDRSIDEGRLVTEAAIFADKTA
                     IDEETVRLHSHVAQYREILELDEPIGRKLDFLTQELNRESNTIGSKCQDVSITRLVVE
                     LKSEIEKIREQIQNIE"
     gene            complement(7018..>8389)
                     /locus_tag="ACZPAX_02940"
     CDS             complement(7018..>8389)
                     /locus_tag="ACZPAX_02940"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015563970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate carboxylase subunit B"
                     /protein_id="HIR1112887.1"
                     /translation="VLRDAHQSLLATRMTMDEMRPILPEMDKIPYFSVECWGGATFDS
                     CIRFLDEDPWERLRILRKELPHQKLQMLFRGQNMLGYRPYADDAVEYFVQKSVANGID
                     VIRIFDALNDPRNLQTAIKAANKEGAHVQACISYTLSPVHNNEYFANYAKTLEEMGAD
                     SICIKDMAGLLKPYTCYDLVKELKETIKIPVDIHSHYTAGLASMSILKGIEAGADIVD
                     TAMSPLALGTSHMPTESLVAALQGTDYDTGLDLNQLNVVRAYFNKLREKYIANGQINQ
                     KSLGVDANTLLYQVPGGMFSNMLKQLKDAGKEDKLQEVLEEIPRVREDAGYPPLVTPT
                     SQIVGTQAVFNVIMGERYKMVTKEFKGLVHGDYGKTPAPIKKEFSDFILKGEEPITCR
                     FADTLEPEMEKLKAEASKYAIQEEDVLTYAMFPQVAPKFFEKRNARLQGVDALHADYE
                     NKSHPV"
BASE COUNT         1586 a         2496 c         2535 g         1772 t
ORIGIN      
        1 ccacgatgta agctgtcagg gcgtccagcg tcatatccga aatgcagatc ctttcgctca
       61 tgcagggtcg cctccttttt ataataacga attttgtgtc actttcgttc caggatggcg
      121 acaaaaaaac cgtccgtccc ggtcttgtgg ggcaagaaca gcgtgcccag tgggccgata
      181 tccgccccgg gtagggctgt ttccggcttc accacatgga aatccgggtt ggcggagaga
      241 aatttttcca cctgagcctg gttctcctta taattgactg tacaggtaga atacaccaaa
      301 cgaccgtttt cggcaagtga atcggtccca ttttgcagga ttttttgctg cgtggcgagc
      361 agattctcta tcccgtccaa atccttgtac cgaatgtccg gctttttggc gataacgccg
      421 aggcccgagc atggcacatc gcagagcacc cggtcgaatt tcggccagcc ggggttatgc
      481 ctggtggcgt cgttttccac cgccacggca caggtcacgc cgcagcggtc aaaggatctc
      541 tgcaaaagcg gcacgcgggt ggggacaaac tccccgctga ccagctggcc cttgtcctgc
      601 atggcctcgg ccagcgtcag gcttttgccg cccggcgcag cgcagaggtc aagcacctgc
      661 tggccgggtt tggcatccac acacagcgcg gcaaactggc tggcaaggcc ctgtacgtgg
      721 aaaagccctt ctttaaaggc cgcgcttgcg gcgggcgaac cggcaaactc tatcaccagc
      781 gccccggccc aggggccggg ctttacggta tggccctcgc tttgcagcag cgccgtcagc
      841 gcggcgtcgt tggtgcgcag ggtgttcacg cggatggtcg ttttcggctt gtcgtaaaag
      901 gcggcggcca gcgggaaagc ctcctcaccg aactgctcat aaaacagctg cgctaccggg
      961 cgggacaagc agtagtacgt tgccagtcgg tccagcgggt cggcaaattc gctctcgcaa
     1021 actttggcgg aagcggcgcg gcgcagcacg gcgttgacca tgcccgcagc actggcctta
     1081 cccagtgcct tcgtcagctt gaccgattca ttcacggcgg cgggcagcgg cacattcatg
     1141 taccgcgcct gggccagccc cgcccgcagc acagcgcgta ccggcgcgtc cagcttgttg
     1201 acaggctttt tcgtgaattg atttaagatg taatccagca gcggtaggcg ttccagtgtt
     1261 gtatagaaaa tacgcgcagc aaaggcggcg tcgcggccat ccaggggcgg cgcacacttt
     1321 ttcagctcag cgtccagcac caggttggcg taaccggcct gttcctggtg catcagggcc
     1381 ttgacggcca ggcggcgcgg cgtcacaggc tgtcccccgc tttcaggcgg cggcctgcgg
     1441 cccaggcagt gcccgccatc ggcttgccgc cctcgggcgt caccgtcagt agagcgaccg
     1501 caccatttgc agcggcaatc gtcagcggct tcagggccag caccgtgccg ggggtggcgt
     1561 tctgggcatt ctcggccaga cggcattcca aaactttgat gcgcttgccg tcgtgctcaa
     1621 accaggcgac cggccagggg ttcatgccgc gcacccagtt gtggatgtgg gcggcgtcct
     1681 gcgtgaaatc aaaacgggcc atgtccttcg tcagcggcgg ggccatggta gcctgggcgt
     1741 catcctgctt ctgcggggtc agctcccctg ccttgatttt ttccagcgcc gtcaccagcg
     1801 ttttcgcgcc cacggcgctg acacggtcaa acagttcgcc gctcgtctcc tcggggtcaa
     1861 tggcgaccgg ttccaccatc agaatatcgc cggtgtccag tccttcatcg agctgcatga
     1921 tggtcacacc ggtcccggca ttgccattta agacagacca ttggatgggc gcggagcctc
     1981 ggtatttcgg cagcagcgag acgtgcaggt tgatacagcc gtacttggcc gcgtgcagca
     2041 gctgcggcgg aatgatgcag ccgtaggcca caacaacgat gatgtcgggg gccagctctt
     2101 taatgacatc gtcggacgaa ccatcccgca gagtgcgggg ctgatacacc ggaatgccgc
     2161 gctccatcgc cagctgtttg accggcgggg ccgttaaaat ctgcttgcgg cccaccggct
     2221 tgtcgcgccg ggtaaagacg gcacagacct cgtgcccggc ccccagcagg gcggccagac
     2281 tttcggcggc aatgtcaggg gtgcccatga acaggatgcg cataaatctt agtcctcgtt
     2341 ttcctcaggg taatcgtcct catagaaatg ctcggccagg tccatgatgg tgatgccgtc
     2401 caggtgattg ttctcgtgct ggatgcagcg ggccagcata tcgtcggcct ccatttcaaa
     2461 ccattcgcca tggcggtcct gggccttgac cttgacatgc ttcgagcgct tgattgcgcc
     2521 gttatgcccc gggaaggaca ggcagccctc atacagctcg acctgctcgt cgctcaattc
     2581 gaggatttca gggttgatga actcgatgaa cttgtattcg taattctcgg ggacttcctc
     2641 gggcaggtcg cggtcgtcca ggcagatgaa aaggcggcgc atcatgccca cctgcgggcc
     2701 ggccaggccc aggccgttgg cggccagcag ggtttccttc atgtcgtcga gcagagtgcc
     2761 caggcggtcg tcaaacttcg tgaccgggcg gcagactttt ttcagaatcg ggtcgccgtc
     2821 ctgtacgatg gtacgcagtg ccataaaaat aacctccaaa tgttataaat caccgtcaga
     2881 attgaaatcg agtaccacgg acgctttctg cggcagcttt tcggcgtcgt aggcgtccag
     2941 tgtctcatgc aaaagcccgc ggaacgctgc atcgttgcgg catttcagcg tgagcttgta
     3001 gcgatatttg ttgttgagca tcgtaatgtt cattggcgca gggcctaaga cccgcagtgg
     3061 gatattcggc ttttgcgcgg cgtgctgggc caacagtgcg ccaaaccgct gcgctgcgat
     3121 gaacaccgca ccctcctggt cgccgacaaa gccgacgacc acaaacgagc agaacggagg
     3181 gtagagacca aacctgcgga aggtgatttc ctcccggtag aagccctcgt aatcctgggc
     3241 tgcggccagc tgcagaaccg ggtggtccgg tacgcaggtc tggatcagcg cccggcccgg
     3301 cagttcggct cggccgccgc gccccacgac ctgagtaatc aggctgaaca cgctctcata
     3361 cgcgcggaag ccctgaccga acagcagtga gtcgataccc aaaacaccca ccagcgtgac
     3421 cttttcaaag tcaaggccct tggccaccat ttgggtgcca agcaggatgt cgtactcctg
     3481 ccgcgcaaac tgggccagca tcgtctcgtg ggcgtcctta cgggcggtcg agtcctgatc
     3541 catccgcaaa atgcgggcat cgggtaaaag ctcggccagc tcttcctcca cccgctgggt
     3601 gccaaagccg ctgtaattga tcttaccgcc gcactcgggg caggtctggg gcaagggctg
     3661 catggtgtgg ccgcagtggt ggcacatcag cgcctgctgc ggtttgtggt agaccagcgg
     3721 cacgctgcaa ttcgggcatt tcagcacatg accgcaggtg gcgcacatac cgatggtgcg
     3781 gtagccgcgc cggttgagca gcagaatact ctgttcgccg ttgtcaatgt tttcccgcag
     3841 ctcccgcgcc agccgcacgc tgacctcgcg ggggttgccc gcggccagct cggcacgcat
     3901 atcgatgaaa ttcacgctgg gcagcggccg cccgccgtaa cggtgggtca gctgcaccag
     3961 ctgcaatttg ccgctctcgg cggcgtggta agtctcggtc agcggcgtgg ccgaggcgaa
     4021 cagcaacaag gcgttctcca tcacggcgcg ctttttggca acgtcgtggg cgtcatagcg
     4081 cggggcggat tcactctggt aggtgtgctc ctgttcctcg tccatgatga taagccccag
     4141 gttttgcagc ggcgcaaaga cagcgctgcg cgtacctacg acgatatcgg cgtttccctg
     4201 ctggatcatc cgccattgca gcagccgctc ggtgttgttg agggccgagt gctgcacggc
     4261 caggcggctg ccgaaggtag acttgagccg ccgtatcatc tgcggtgtca ggctaatttc
     4321 cggcaccagt accagtgcgc ggcggcccaa ttccagcgtg cgctggatga gctttagaaa
     4381 cacgatagtc ttgccgctgc cggtcacgcc gtgtaaaagt gccgcgtggg gcttgaaatc
     4441 tgctaagttt ggcagcaggt tgtcaaaaac ctgctgctgt tcaggggaaa gctccaacgg
     4501 ctgcgggtca aaggggatgg ccgcaaacag gtcaagggat ttgtcctgtt cggcggcggt
     4561 cagcacgccc tttttcacaa ggccgtccag cgtcgccttg gagatgccca cctcgtccag
     4621 ctgtcgggcg gtcagggggc cgcttttcag cgcttccacg gcggccagct ggcgcgggcc
     4681 gggcttcggc ttttcgggca gctcccccgc cagcgtgtac agccgctcgg tcgagcggcg
     4741 cagacggctc tgcatgacgt gcttaccgtc cacgacggca gggcggtact gcgcaccgta
     4801 agggattacc gctttgacgg cctcgaacca ggtgcagaag gtgcgctctt tcaaaaagcg
     4861 caccagctcc aacaaatccg gcgtcaggcg agcgtcctcg ggcgcggcgt cgtacagggt
     4921 tttcagccgc ttgggggcgt cagcctcgtc cacttcctcc acagccagca caacggccat
     4981 ccgggctttg tcaccccggc caaacggcac caacaccatg ctgccgggaa aaaccctgcc
     5041 gaccagctgt gtaggaatgg tgtaggaata cagccggtca aagtggatgt tcgcgtcgtt
     5101 tacggcggct tgggcaagtg tcagcgtcat tcgtcgctgc cgtgctgcag cttgaactgg
     5161 ataatggagt agaggctctc ggcgcaggag tcgacctcga cgttgacaac gtgctcatca
     5221 tagttgacgg agttcgcaat ctcggcctcg gcacgtttca ggcggcgctg gatggtttcc
     5281 tcatcctcgg tgccgcggca gcgcagacgg cgttccaact cctgcatatc cggaggcagc
     5341 acaaaaatcg ttgtggcacc ggggtatatt tttttgatgt tgcctgcgcc ctcgacctcg
     5401 ataaccagaa tgaccgggat gccaagcttc atgcggccct cgatctcact gcgcagggtg
     5461 ccatagtagt tctcgcaata tttcgtgtgc tcgacaatct ggtcatgggc caggtgatcc
     5521 tcaaaatccg ccaccgacat aaagtggtag ttgatgccgt ccttctcgcc ctcgcgcggg
     5581 gctcgggtgg tggcggagac ggtgtgctgg atctccgggt gctttttcag cagtgcggcc
     5641 acgacggtat ccttgccagt gccggagggg ccggagacaa caaacagata tttttcacct
     5701 ttcatcgtta cttcctcttt tcttccgttt cttctgcggc acgcccggta atgctttcca
     5761 gcgggatgcc cgataatatg acatggtcag agtccatgat gagcacagcc tgagtgcgcc
     5821 gcccatagct ggcgtcgatg agggcgccct tctcacgggc gtcctgcacc aggcgcttga
     5881 tgggcgcaga gtccgggctg acgatgccga tgatgcggtc ggcgttgacc atattgccaa
     5941 agccgatgtt cagcagcttc atggcgtacc tcccctgtta ctcgatgttc tggatctgct
     6001 cgcgaatctt ttcaatctcg gatttcagct ccacgaccag ccgcgtgatg gagacatcct
     6061 ggcacttgga gccaatcgtg ttggactcac ggttcaattc ctgcgtcaaa aagtccagct
     6121 tgcggccaat cggctcgtcc agctccaaaa tttcgcggta ctgtgccaca tggctgtgca
     6181 ggcggacggt ctcctcgtcg atggcggtct tgtcggcgaa gattgcagcc tcggtcacaa
     6241 gacggccctc gtcgatgcta cggtcgccca gaagctcctg caggcgggca tagagcttgt
     6301 cgctgtacgc ctgcacgcgg cccgcgctgt tctcctcaat ttggccgacc agcgtctcaa
     6361 tggtggtcag gcggctcgca acatcggcct tcattttctc gccctcgacg gcgcgcatgg
     6421 aaatgaaggc gtccacggcc tgctcggcca cggatttgac atcctcccac agctcatcct
     6481 cgttcgttgg agcggcggtc tgagtcagca cgtccggaaa gcgggaaagc atcccgaccg
     6541 agacgtcgtt tttcaagtcc atccgctccg ccatggcgtt gagcgcggcg cggtagccct
     6601 cggccagctg ccagttgaca gtgacgacgg tgtccgccgc tgtgacggtc tggatggaaa
     6661 ggctcagctc caccttgccg cggctgacct tggcggcgac gagctttttc agcttatctt
     6721 ccaaaaatgc gcaggagcgc ggcaggcgcg aggaatactc aaaaaatcgt gcattgacgc
     6781 tgcgaagttc cactttgatg tcacggccgt gcagcagcgc acccgcgcgg ccataccctg
     6841 tcatgctcaa aaccataacc ttacacctca taaaacacat atattgtata ttgtactatt
     6901 tttgggggcc agatgcaagg ctggcagtcg tattttgcaa aatttcacgt ggatttttac
     6961 aatttatcca aaacaaaaag ccgcccccgc agcaaagcgg agacggcata gtgccaatta
     7021 gacgggatga gacttgttct cgtagtcggc atgcagagca tccacaccct gcaggcgggc
     7081 gttgcgcttc tcgaagaact tgggcgcaac ctgcgggaac atggcgtagg tcaagacgtc
     7141 ctcctcctgg atagcgtact tggaggcttc agccttcagt ttctccatct cgggctcgag
     7201 ggtgtcggca aagcggcagg tgatgggctc ctcacccttc aggatgaagt cgctgaattc
     7261 cttcttgatg ggcgcggggg tcttgccgta atcgccatga accaggccct tgaactcctt
     7321 ggtgaccatc ttgtagcgct cacccatgat gacgttaaag acagcctggg tgccaacgat
     7381 ctggctggtc ggggtgacca gcggcggata accggcatcc tcacgcacgc ggggaatctc
     7441 ctccagaacc tcctgaagct tgtcctcctt gccggcgtcc ttcagctgct tgagcatatt
     7501 ggagaacata ccgcccggca cctgatacag cagggtattg gcgtcaacgc ccagggactt
     7561 ctggttgatc tggccgttgg cgatgtactt ctcgcgcagc ttgttgaagt aagcgcggac
     7621 gacattcagc tggttcaggt caaggccggt gtcgtagtcg gtgccctgca gggcggcgac
     7681 caaactctca gtgggcatat gagaggtgcc cagagcgagg ggactcatgg cggtatcaac
     7741 gatatctgcg ccagcctcga tgcccttcag aatggacatg gaagcaagac ctgcggtgta
     7801 atggctgtgg atatccacag gaatcttgat ggtctctttc agctccttga ccaggtcata
     7861 gcaggtgtag ggcttcagca gaccggccat atccttgatg cagatagagt cggcacccat
     7921 ctcttccaga gtcttggcgt agttggcgaa gtactcgttg ttatgtaccg ggctcaaggt
     7981 gtagctgatg caggcctgaa cgtgagcgcc ttccttgttg gcggccttga tggcggtctg
     8041 caggttgcgc gggtcgttca gagcgtcgaa aatacgaatg acgtcaatgc cgttggcgac
     8101 agacttctgg acgaagtact cgacggcatc atcggcgtag ggacggtagc ccagcatatt
     8161 ctggccgcgg aacagcatct gcagcttctg gtggggcagc tccttgcgca ggatgcgcag
     8221 gcgctcccaa ggatcctcat ccagaaaacg gatgcaggag tcaaacgtag cgccgcccca
     8281 gcactccaca gagaagtacg ggattttgtc catttcgggc aggatggggc gcatttcgtc
     8341 catggtcatg cgggtggcca gcagagactg gtgggcatca cgcaggacg
//
LOCUS       DBMDSQ010000047         8330 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_2328_length_8330_cov_1.640297, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000047 DBMDSQ010000000
VERSION     DBMDSQ010000047.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 8330)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8330)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8330
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2328_length_8330_cov_1.640297"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(213..1118)
                     /locus_tag="ACZPAX_02945"
     CDS             complement(213..1118)
                     /locus_tag="ACZPAX_02945"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015564683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HIR1112888.1"
                     /translation="MADLHVDFLGKRLAGPVVAASGTFGFGPEYAGIEDLRVLGGISG
                     KGLTLHGQPGNEGERLYETPSGLMNSIGLQNPGVQHFIDHELPAMRQCGTTVWANLGG
                     HTIDENVEGVMMLCAAGVDFIELNISCPNVKQGGLAFGIRAADASEVVSAVRKVCNVP
                     LIVKLSPQAESIPEMCKAVEAAGADAISLCNTFQACAIDLEKRRPVFNNTFAGLSGPA
                     VRPIALRMVWQAVGAVSIPVVGLGGILTGRDALEFIMAGAAAVQVGTANFLDPKACTR
                     ITSEINQWMDAHGVKTLEEIRGCAR"
     gene            complement(1118..1888)
                     /locus_tag="ACZPAX_02950"
     CDS             complement(1118..1888)
                     /locus_tag="ACZPAX_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005929274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HIR1112889.1"
                     /translation="MNVTSCNLTVLRQEVVAADIMRLVVQWQDPSREPHAGQFYMLRA
                     WAADATPILSRPISVDDFDHQTGALTFLYQVKGEGTRKLAALQAGNALTVTGPCGNGF
                     DTAALAASHKRIAVVGGGIGTAPLLLLCKELCRAGVRPDLYVGFRDASYGMESFVPWC
                     HTIHLATDSGSEGHHGLVTDLLDVRNYDIVLTCGPMVMMRGVAKLCADAGVPCLASLE
                     KKMACGLGACLGCTCHTKIGPVTVCKDGPVFNAQEVFD"
     gene            complement(1869..2732)
                     /gene="pyrF"
                     /locus_tag="ACZPAX_02955"
     CDS             complement(1869..2732)
                     /gene="pyrF"
                     /locus_tag="ACZPAX_02955"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015564681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HIR1112890.1"
                     /translation="MLTNMDKLYESVAANGPVCVGLDTEISYLPTTDTARTAGENVVA
                     FNRALIDATKGVAGCYKVQIAYYESLGLDGMRAYAETLKMARATGLPVIADIKRGDIA
                     KTAEMYAKAHFTGDFEADIITLAPYMGLDSISPYLPYCAEQGKGVFVLCRTSNPGAKD
                     FEYKKLDDGRHVYDLVGDSLTALGKDYMGEHGYSSIGLVIGGTHTEEATAIRAAYKDT
                     FFLIPGYGAQGGKAADIAQYLTKGNGGVVNSSRGILLAYKKQPGVAFDEAAYNECVRM
                     REDIQGECNKL"
     gene            complement(2907..4097)
                     /locus_tag="ACZPAX_02960"
     CDS             complement(2907..4097)
                     /locus_tag="ACZPAX_02960"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015564680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HIR1112891.1"
                     /translation="MLIRNAKNSAGKPVEVWLCDGKIAAVGLGLLVPEGETVLDAKGR
                     TVLPAFIDTHCHWRTPGFEYKEDISTGSAAAAAGGYTFVNLMPNTKPVCSSAEIAHAV
                     EAEAARVGLCDANQTVSITKDFDGKTLDHLLTLPEDVKFITEDGNGVMNNAVMARAFA
                     IATERGLTIMSHAEDREISPWDYRLAENIETVRNCHLAEYYGTRLHMCHVSTKESVDA
                     VRMSRMKGARVSCEVTPHHLWFDDSRLTYRVNPPIRKADDVNALIDAIKDGTVTCIGT
                     DHAPHTAEEKEKGAAGMVGLETAFGVCYTKLCREQRIPLEMLSFLMSAGPAAVLGLAD
                     HKGLLEPGYDADVVLVDIDHMYEVHADELHSKSKNCPYDGALLYGKVVTTIKGGKVTF
                     QIEE"
     gene            4570..5220
                     /gene="epsC"
                     /locus_tag="ACZPAX_02965"
     CDS             4570..5220
                     /gene="epsC"
                     /locus_tag="ACZPAX_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015564677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HIR1112892.1"
                     /translation="MGLWEDAKNIRDKDPAARNVAEVIILYPGFHVLVTHKIAHFLYN
                     HKCFFLARFVSQLARHLTGIEIHPGAKIGRKLFIDHGMGIVFGETAEIGDNCTIYHGV
                     TLGGTGKDTGKRHPTLGNNVLIGAGAKVLGPVYIGDNVRVGAGSVVLKNLPANATAVG
                     VPAEVVRVGRVKCVPADDLDQQDLPDIVNQRISELEARLSRLEAEKQGEFPPDKKE"
     gene            complement(5430..7262)
                     /locus_tag="ACZPAX_02970"
     CDS             complement(5430..7262)
                     /locus_tag="ACZPAX_02970"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007045845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR1112893.1"
                     /translation="MSLETKRIKELFGYAQTLTKEQGVGVMAGRAVGFFKRRFFGKRA
                     RYLPAKQTLDAQRADPAAKTWPTISILTPLYNTPPQFLQQFLNSVQVQTAPNWQLVLV
                     DASDDAHPDVAKTVQARAAQDKRIVYAKIENKGIAANTNEAAKLATGDYLALADHDDM
                     LAPHAVYCMGKALAESGAAFAYSDEALFEKTPKHPRVGHFKPDYAPEYLMAVNYICHL
                     AVFKKSLFNAVGGERPTCDGAQDHDLFLRLIDEMQRTDPAAKPLHIPQVLYYWRVHAA
                     STSGGTAAKPYVEQAAQRAVADHLAATGRQGRVEAGRFPGTCHVVWDIPEPEPLVSIL
                     IPNKDHTDDLEKCLHSIYAKTTYENFEVIVIENNSTDPATTAYYKKMPDRYDRARVVT
                     YKGGFNFSRINNFGRKYAAGKYLLLLNNDVEVINGDWLTQMVGECSQPGVGICGAMLY
                     YPDDTIQHAGIITGLGGYAGHSHKYHKRGGSGYMFRLATVQDFSAVTAACLLVRTAVY
                     DEVHGLDEEFTVAYNDVDFCLRVRDAGYRIVWTPYAELYHYESKSRGSDENDPAKKAR
                     FDAEHDRLYAVHGRDNILHDPYYSPSLSLDYEDFRESGDLRNLK"
     gene            complement(7252..7776)
                     /locus_tag="ACZPAX_02975"
     CDS             complement(7252..7776)
                     /locus_tag="ACZPAX_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007491170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HIR1112894.1"
                     /translation="MKPKRKINRLTEYDYAQNGAYFITICTHERQRILWGNDTFTRAE
                     NVGASIARPQHPPALSKAGKVVEQCIWEIPKQYPHISLEKYAIMPNHVHLLLLIQNGP
                     GGRPMVAPTISTVIQQFKGVVSKRLSRPIWQKSFHDHVVRTEHGCAQIWQYIDTNPKL
                     WHKDCFYEEPPHEP"
     gene            complement(7776..>8330)
                     /locus_tag="ACZPAX_02980"
     CDS             complement(7776..>8330)
                     /locus_tag="ACZPAX_02980"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007045843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR1112895.1"
                     /translation="NDIEILSTAFLRELLSYSQRPDVGAVGAKLYYPDDTIQHAGVLM
                     GINGSAGHSHKSYPRTAVGDMYRLVTTQDYMAVTGACLMTKTALYRAFGGLDEEKFAV
                     AYNDVDYCLKLWQKGLLNVYTPRAEAYHYESKSRGLDTLSENTKRYEREKANFYAKYQ
                     QYIDNYDPYYNPHFNNLFENFGLK"
BASE COUNT         1567 a         2404 c         2570 g         1789 t
ORIGIN      
        1 gttattcaac ttatagtagc atattttgta taaaaatgca agatatatga atgcttatct
       61 tggtgacagc ttcccactta gggggaagct gtcggcggcc ctgaccgccg actgatgagg
      121 ggcggccttg ccgttgctgc ccgtgaaggg gcggcggtgc aagcatgccc ctcatccggc
      181 cttcggccac cttcccccta gggggaaggc ttttatcttg cgcagccgcg gatctcctcc
      241 agcgtcttga cgccgtgagc gtccatccac tggttgatct cgctcgtgat gcgggtgcag
      301 gctttcgggt ccaggaaatt cgcggtgccc acctgcacgg cggccgcacc ggccatgatg
      361 aactccagcg cgtccctgcc ggtcaggatg ccgcccagcc ccacgaccgg gatgctgacg
      421 gcccccacgg cctgccagac catgcgcagg gcaatcggcc gcaccgccgg gccggacagg
      481 cccgcgaagg tgttgttgaa caccggacgg cgcttttcca gatcgatggc gcaggcctgg
      541 aaggtgttgc acaggctgat ggcgtccgcg cccgcagcct cgacggcctt gcacatctcg
      601 gggatgctct cggcctgggg gctcaatttg acgatgagcg gcacattgca gactttgcgc
      661 accgcagaaa cgacctcgct tgcatccgcc gcgcggatgc cgaacgccag accaccctgc
      721 ttgacattcg ggcagctgat gttcagttca atgaaatcca cgcccgcggc gcagagcatc
      781 atgacgccct cgacgttctc gtcgatggtg tggccgccca ggttggccca gacggtagtg
      841 ccgcactggc gcatggcggg cagctcgtgg tcgataaagt gttgtacgcc ggggttttgc
      901 aggccgatag agttcatcag gccggagggc gtctcgtaca ggcgctcgcc ctcgttgccg
      961 ggctggccgt gcagggtcag ccccttgccg gagataccgc ccagcacccg caaatcctcg
     1021 atgcccgcgt actcgggacc gaagccgaag gtgccgctgg ccgcgacgac cggcccggcc
     1081 agacgcttgc cgagaaaatc aacgtgaaga tctgccatca gtcaaacacc tcctgtgcgt
     1141 taaagaccgg gccgtccttg cagaccgtga ccgggccgat tttcgtgtgg caggtgcagc
     1201 ccaggcaggc acccaaaccg caggccatct tcttttcaag gctggccagg cagggcacac
     1261 ccgcgtcggc gcagagcttg gccacgccgc gcatcatgac catggggccg caggtcagca
     1321 caatgtcata gttgcgcacg tccagcaggt ccgttaccag gccgtggtgt ccctcgctgc
     1381 cggagtcagt ggccaggtgg atagtgtggc accagggcac aaagctctcc atgccatagc
     1441 tggcgtcgcg gaagccgacg tagaggtcgg gccgcacgcc cgcgcggcac agttccttgc
     1501 agagcagcag cagcggcgcg gtgccgatgc cgccgcccac gacggcaatg cgcttgtggc
     1561 tggcggccag cgcggcggtg tcaaagccgt taccgcaggg gcctgtcacg gtcagggcat
     1621 tgccagcctg cagcgcagcc agcttgcggg tgccctcgcc cttgacctga tacaaaaagg
     1681 tcagtgcgcc ggtctggtga tcaaaatcgt ccacgctgat ggggcgggac aaaatcggcg
     1741 tggcgtccgc tgcccaggcg cgcagcatat aaaactggcc cgcatggggt tcgcggctgg
     1801 gatcctgcca ctgcacgacc aggcgcatga tatcggctgc gacaacttcc tgacgcagga
     1861 ccgttaaatt acagcttgtt acattcacct tggatatcct cccgcatgcg cacgcactcg
     1921 ttgtaggcgg cctcgtcaaa ggcgacgccg ggctgtttct tgtaggccag caggatgccg
     1981 cggctggaat tgacaacgcc gccgttgccc ttggtcaggt actgggcaat gtcggccgcc
     2041 ttgccgccct gcgcaccgta gccgggaatc aggaagaagg tgtccttgta ggccgcgcgg
     2101 atggcagtag cttcctcggt gtgggtaccg ccaatgacca ggccgatgct ggaatagccg
     2161 tgctcaccca tgtagtcctt gcccagggcg gtcaggctgt cgccgacgag gtcatagacg
     2221 tggcggccat cgtccagctt tttgtactca aaatccttcg cgccggggtt ggaggtgcgg
     2281 cagaggacga acacgccctt gccctgctcg gcgcagtacg gcagataagg gctgatggaa
     2341 tccaggccca tgtagggggc cagtgtgatg atatcggcct caaaatcgcc ggtaaagtgc
     2401 gctttggcgt acatttcagc agtcttggca atgtcaccgc gcttgatatc ggcaatgacc
     2461 ggcaggccgg tggcgcgggc cattttcagc gtttcggcgt aggcgcgcat accgtccagg
     2521 cccagggact cgtagtaggc gatctgcact ttgtagcagc ccgccacgcc cttggtggcg
     2581 tcaatcaggg cgcggttgaa cgcgacgaca ttctcccctg ccgtcctggc ggtgtcggtg
     2641 gtgggcaggt agctgatttc ggtatccagc cccacgcaga cagggccgtt cgcggcaacg
     2701 ctctcgtaca gtttgtccat gttggtaagc atgctgattc ctccgtttga tgtatacaaa
     2761 ttgtggcttt gtggctctgc aatattatat aatgttggaa atcgtagggg cggattctat
     2821 atccgcccgc gggggtgtgc cagcggcagc cttgcacggg cgcataggga atgcgcccct
     2881 acgggtgttt tgtgcgtgaa gcggcgttac tcttcaatct ggaacgtaac ctttccgcct
     2941 ttgatcgttg ttacgacctt gccgtagagc agcgcaccgt cataagggca gttcttgctc
     3001 ttgctgtgca gctcgtcggc gtggacttca tacatatggt cgatatccac cagcacgaca
     3061 tcggcgtcgt agcccggttc cagcaggccc ttgtggtcgg ccaggcccag cacggcggcg
     3121 gggcctgcgc tcatcaaaaa gctgagcatt tccagcggga tgcgctgctc gcggcagagc
     3181 ttggtgtagc agacgccgaa cgcggtttcc aggcccacca taccggcggc tcccttctcc
     3241 ttttcttcgg cggtgtgggg agcatggtcg gtgccgatgc aggttacggt gccgtctttg
     3301 atggcgtcaa tcagtgcgtt cacgtcgtcc gccttgcgga tgggcggatt gacgcggtag
     3361 gtcaggcggc tgtcgtcaaa ccacaggtga tggggcgtga cctcgcagct gacccgcgca
     3421 cccttcatcc ggctcatccg tacggcgtcc acggattcct tcgtggagac gtggcacata
     3481 tgcagccgcg tgccgtaata ttcggccagg tggcagttgc gcaccgtctc gatgttctca
     3541 gccaggcggt agtcccatgg gctgatctcg cggtcctcgg cgtgggacat gatggtcagg
     3601 ccgcgctcgg tggcgatcgc aaaggcgcgg gccatgacgg cattgttcat gacgccgttg
     3661 ccgtcctcgg tgatgaattt gacatcctcg ggcagggtca gcagatgatc cagcgttttg
     3721 ccgtcaaaat ctttcgtgat ggagacggtc tggtttgcgt cgcacagccc cacgcgggcg
     3781 gcctcagcct cgacggcgtg ggcaatttcg gcggaagagc agacgggctt ggtgttgggc
     3841 atcaggttca cgaaggtata cccgcccgct gctgctgcgg cagagccggt ggagatatcc
     3901 tctttatact caaagccggg ggtgcgccag tggcagtggg tgtcgatgaa ggcaggcagc
     3961 acggtacggc ccttggcgtc gagcaccgtc tccccttccg ggacaagcag ccccaggccg
     4021 acagcggcga tcttgccgtc gcacagccag acctcaacag gtttgccggc gctgttcttt
     4081 gcgttgcgga tcagcatggt gggaacctcc ttaccatttc cttttgccca aaggccccta
     4141 gggtatttgg gcaaaaaaaa acttttccca aaagggaaaa gtcaaaaagt cacactcaaa
     4201 tcaacggcgg gaaaaaacac tgcaccgtga attttgaagc aagaaacaaa ctgcatcatc
     4261 attctggcaa gctccttccc atcattttta cataggttta acttattgta ccatgcacac
     4321 gcggcagtgt caatgcaaac gcccggattg cgaaagtaag gatttcctga atttttgcgc
     4381 cgacttttgt acaaattgat aaaagtgtat ttacagcgtg tgccgatgcg gccaacctta
     4441 cagcgccccc tcatttttgt gtggtttggg ttcagtatac tgtgcagccc gattttttgc
     4501 aagcattgca cccggcacaa aaatatgcta tactataaat cattatggtg aagttaagag
     4561 gaatgaacta tgggcctttg ggaagatgca aaaaatatcc gcgataaaga ccccgccgca
     4621 cgcaatgtgg cggaggtcat tatcctgtat cccggctttc atgtgctggt cacgcacaag
     4681 attgcgcatt ttttgtacaa ccataagtgt ttttttctgg cgcggtttgt cagccagctg
     4741 gcacgccacc tgaccggcat tgaaattcac cccggtgcca aaatcggccg caagctcttt
     4801 atcgaccacg gcatgggcat cgtctttggc gaaacggcgg aaatcggcga caactgcacg
     4861 atctaccacg gtgtgaccct gggcggcacc ggcaaggata ccggcaagcg ccacccgacg
     4921 ctgggcaaca acgtcctgat cggcgcgggg gccaaggtgc tgggcccggt ttatatcggc
     4981 gataacgtcc gtgtcggcgc gggcagtgtg gtgttgaaaa acctgcccgc caacgccacc
     5041 gccgtcggcg tccccgccga ggtcgtgcgg gtcggcaggg tcaagtgcgt cccggcggac
     5101 gacctggatc agcaggatct gccggatatc gtcaaccagc gtatctccga actggaagcc
     5161 cgcctgagcc gcttagaggc cgaaaagcag ggcgagttcc cgccggataa aaaggaataa
     5221 taaaaacgca cgccgtgaaa tttacggcgt gcgttttttg tcagtatggc ttccccaaag
     5281 gggggaagct gtcagcggcc ccgcccactg actgatgagg ggcaaactta ccgggaattt
     5341 cccactgacg ggcaataggg caaacccgcc cctcatccgc cctcggtcgg ggcctcgggc
     5401 accttccccc aggggggaag gcaatcaact catttcaaat tccgcaaatc tccgctctcg
     5461 cggaaatctt catagtccag agacagtgac gggctgtaat aggggtcgtg caggatgttg
     5521 tccctgccgt gtacggcata caggcggtcg tgctcggcgt caaaacgagc ttttttggcc
     5581 gggtcgttct cgtccgagcc gcggctcttg ctctcataat ggtaaagctc ggcatagggc
     5641 gtccagacga tgcgatagcc ggcatcccgc acccgcaggc agaagtccac gtcgttgtag
     5701 gcgacggtga actcctcgtc gaggccgtgc acttcgtcat aaacggcggt gcgcaccagc
     5761 aggcaggcgg ccgtcacggc cgaaaaatcc tgcacggtgg ccaggcggaa catatacccg
     5821 ctgccgccgc gcttgtggta cttgtggctg tggcccgcgt agccgcccag cccggtgatg
     5881 atgcccgcgt gctggatcgt gtcgtcaggg tagtagagca tcgcgccgca gatgccgaca
     5941 ccgggctggc tgcactcgcc caccatctgg gtgagccagt cgccgttgat gacctcgacg
     6001 tcgttgttca gcagcaacag atacttgccc gcagcgtact tgcggccaaa attattgatg
     6061 cggctgaaat tgaacccgcc cttgtaggtc acgacccggg cgcggtcgta gcggtcgggc
     6121 atttttttat agtaggccgt tgtggcgggg tcggtggagt tgttctcaat gacgatgacc
     6181 tcaaaatttt cataggtcgt cttggcgtag atgctgtgca ggcatttttc cagatcgtcg
     6241 gtgtggtcct tgttgggaat taaaatcgat acgagcggtt ccggctcggg gatgtcccag
     6301 acgacgtggc aggtgccggg gaagcgcccg gcctccaccc tgccctgccg cccggtggcg
     6361 gccaggtggt ccgccacagc gcgctgggcg gcctgctcga cataaggctt ggccgctgtg
     6421 ccgccgctgg tggatgccgc gtgtacacgc cagtagtaga gcacctgcgg gatgtgcagc
     6481 ggcttggctg cggggtcagt gcgctgcatt tcatcgatga ggcgcaggaa taagtcgtga
     6541 tcctgcgcac cgtcgcacgt gggtcgttcg ccgccgacgg cattgaacag cgattttttg
     6601 aagacagcca ggtggcagat gtagttgacc gccatcaggt actcgggcgc gtagtcgggc
     6661 ttgaagtgac cgacgcgggg atgcttgggc gttttttcaa acagggcctc gtcgctgtac
     6721 gcgaaggctg caccgctctc ggccagggcc ttgcccatgc agtagacggc gtggggggcc
     6781 agcatatcgt cgtggtcggc cagggccagg tagtccccgg tggcgagctt ggcggcctcg
     6841 ttcgtgttgg cggcgatgcc cttattctca attttggcgt agacaatgcg cttgtcctgt
     6901 gcggcgcggg cctgcacggt tttggcaacg tcggggtggg cgtcatcgct ggcatccacc
     6961 agcaccagct gccagttcgg cgcggtctgg acctgcacgc tgttcaaaaa ctgctgcaaa
     7021 aactgcggcg gcgtgttgta cagcggcgtc aggatgctga tggtgggcca ggtcttagcg
     7081 gccgggtcgg cacgttgagc gtctaatgtc tgttttgcgg gcagataccg cgcacgtttg
     7141 ccgaagaacc gccgtttaaa aaagccgacg gcccgcccgg ccataacgcc gacgccctgt
     7201 tctttcgtca gggtctgcgc atagccgaac agctctttga tgcgttttgt ctcaaggctc
     7261 atggggtggc tcctcataaa agcaatcttt gtgccagagt ttggggttcg tgtcgatata
     7321 ctgccaaatt tgtgcgcagc catgttcggt gcgtacaaca tggtcatgga aagatttttg
     7381 ccagatgggg cgggaaagcc gcttggaaac gacaccttta aattgctgta taacggtaga
     7441 gattgtaggg gcgaccattg gtcgcccgcc cggcccgttc tggattaaca acaacaaatg
     7501 aacatggttt ggcattatgg cgtacttttc taacgagata tgcggatact gctttggaat
     7561 ttcccaaata cactgctcta caacttttcc tgctttggac aacgcgggtg ggtgctgcgg
     7621 gcgagcaatg ctcgccccta cattctcggc tctcgtgaac gtatcatttc cccacaaaat
     7681 cctctgtcgt tcgtgcgtgc aaatcgtgat gaaatacgcg ccgttctgtg cgtaatcgta
     7741 ctccgtaagg cggttgattt tccgttttgg cttcatcact tcaacccgaa attctcaaac
     7801 agattgttga aatgtgggtt gtaatagggg tcataattgt cgatatactg ttgatatttt
     7861 gcgtagaaat tcgccttctc acgctcgtag cgcttggtgt tctcgctcag ggtgtcaagg
     7921 ccgcggctct tgctctcgta gtggtaggct tcggcgcggg gcgtgtagac gttcaaaagg
     7981 cctttctgcc acaacttcag gcagtagtcc acgtcgttgt aggcaacggc gaacttttcc
     8041 tcgtccagac cgccaaatgc acggtagagt gcggtttttg tcatcaggca ggcaccggtc
     8101 acggccatgt aatcctgcgt cgtgaccagg cggtacatat cgccgacggc cgtgcggggg
     8161 taactcttgt ggctgtggcc cgcgctgccg ttgatgccca tgagcacgcc cgcgtgctgg
     8221 atcgtgtcgt cagggtagta gagctttgcg cccaccgcgc ccacatcggg ccgctggctg
     8281 taactcagca gttcgcgcaa aaaggcggtc gacaaaatct cgatatcatt
//
LOCUS       DBMDSQ010000048         8268 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_2349_length_8268_cov_1.535080, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000048 DBMDSQ010000000
VERSION     DBMDSQ010000048.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 8268)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8268)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8268
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2349_length_8268_cov_1.535080"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="ACZPAX_02985"
     CDS             complement(<1..225)
                     /locus_tag="ACZPAX_02985"
                     /EC_number="3.5.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015565333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004132 - dCMP deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006220 - pyrimidine nucleotide metabolic
                     process [Evidence IEA];
                     GO_process: GO:0009972 - obsolete cytidine deamination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxycytidylate deaminase"
                     /protein_id="HIR1112896.1"
                     /translation="MKRQDYISWDEYFMGIALLSAMRSKDNNSQVGACIVSPENKILS
                     LGYNGMPIGCSDDDMPWEREGEDLDTKYMYV"
     gene            complement(256..531)
                     /locus_tag="ACZPAX_02990"
     CDS             complement(256..531)
                     /locus_tag="ACZPAX_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcriptional regulator SpoIIID"
                     /protein_id="HIR1112897.1"
                     /translation="MKGDPEERAAAVGRYIAESGATVRAAAAVFGVSKSTIWKDQHRL
                     RRQNPGLWAEVQAVVQKNKAERHLRGGEATRRKYLREPSCTAPHGTV"
     gene            624..1013
                     /locus_tag="ACZPAX_02995"
     CDS             624..1013
                     /locus_tag="ACZPAX_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014119130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HIR1112898.1"
                     /translation="MTFERYPELDTLLLANPGAVRDYKPEWGWDRYMVGGKMFAALLT
                     TADDCAPEYQNHPMLTVKVAPEEGNFLQRQYPDILPGYYMNKQHWISVRLDGAVPQEE
                     VMRLCKSGYALIFAKLTKKMQKEIFGE"
     gene            complement(1053..2930)
                     /locus_tag="ACZPAX_03000"
     CDS             complement(1053..2930)
                     /locus_tag="ACZPAX_03000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1112899.1"
                     /translation="MLLSILYLALFIYVGLLLARRAVPDGSAAVILPLGCGFGVSLLA
                     VLPAVLALALGFTLPAVLLAAAAAGVLGGVLLRRGVRLRGMAKDANCGALWACILPVA
                     LITLYLLHTHVLHMVNGAYHTGQSCYGDMPMHLGFIKYIAQSGEFLPCYPLLGGEHRF
                     GYPFLCETVSSVFLVLGTDLRTAYLLPMLPAFFSVYSMFWQLAHRMTGSAVKASLAFY
                     LFFMGSGFGFVYFLSSAEKFAGIFTGFYTTPTNFVEQNIEWVNPIVDLLIPQRATLFG
                     WCVLFPAVYLLWRFCYEGQRRLWPWLALLALPLPLLHTHSALALVLLCLVGGVYTLAQ
                     GARRETLLPWLGLAAVCGAAWLAQMLPTVLAQSLNGQHMLRLHFNWINGQDNGTLKDN
                     YFWFYIKNIGLVYLLLVPAFIHAKSKQRWLYGGGLAILALAEFVVFQPNNYDNNKLLY
                     VWHMLGCILAAQLLVDLFAKVRALPWRALGLAACCFAAMFGSVLTVGREIFIDYQHWS
                     VNDIALADYIDANAESDALFLTSDSHVTPVFALAGRRILCGSGSYVYYHGMDYADEYR
                     AMAALYEHPNEGTLAAWDVGYVLFDSSVYGKFSDADESWYAARYPIWYENDGCRVYKI
                     V"
     gene            complement(2933..3847)
                     /locus_tag="ACZPAX_03005"
     CDS             complement(2933..3847)
                     /locus_tag="ACZPAX_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009323590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S cluster-binding domain-containing
                     protein"
                     /protein_id="HIR1112900.1"
                     /translation="MQLPHHCTLCPRACGADRAAGQRGLCGADDALRVARAALHHWEE
                     PCLSGDPSAETGSGTVFFSGCTLRCCYCQNYPISQGEVGKEISVERLAEIFMNLQNGG
                     AKNLNLVTASQYLPWVTAALDQARTAGFCLPVVYNTGGYETVEAVKALAGYVDIWLAD
                     YKYADGALAAELSAAQNYPAVADAALRQMLLQTGAPVYDADGYLQKGVIVRHLALPGH
                     AADSKAVLQRLAALRAETGVAFIPSLMSQFTPFYKAAEHGLGRRITTYEYRQVIDEAV
                     RLGLTDGYMQEKSSAREEYTPPFDLEGV"
     gene            3952..4683
                     /gene="deoD"
                     /locus_tag="ACZPAX_03010"
     CDS             3952..4683
                     /gene="deoD"
                     /locus_tag="ACZPAX_03010"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HIR1112901.1"
                     /translation="MSTPHNRAEKGDFAKTVLMPGDPLRAKFIAETFLENPRVVTDVR
                     GMLGYTGTYQGRPISVMGSGMGMPSIGIYSYELFSQYGVENIIRIGSAGSYTDKAKLF
                     DVVLATGAYSESNYAVTQSGDTDDIQTPSAELNDALRASAKAADIPLIEGNIHSSDVF
                     YRQPSDAKPTYWEKLRDEKGCLAVEMESFALFHNAKVLGKRAACLLTISDSFVSPEIT
                     TAEQRQTSFTAMMKVALGVDAFLEK"
     gene            4685..5104
                     /locus_tag="ACZPAX_03015"
     CDS             4685..5104
                     /locus_tag="ACZPAX_03015"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HIR1112902.1"
                     /translation="MEKAEIQALIREAFEGQKFAYVPYSRFHVGAALRARDGRVFKGC
                     NIENAGYTPTNCAERTALFKAVSEGVREFDAIAIVGSKVGEKNTLVTGPCGVCRQALY
                     EFGGPKLTVIMAKDENDYIVTTLGDLLPYGFGPANLE"
     gene            5167..6330
                     /locus_tag="ACZPAX_03020"
     CDS             5167..6330
                     /locus_tag="ACZPAX_03020"
                     /inference="COORDINATES: protein motif:HMM:NF014647.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR1112903.1"
                     /translation="MKKFLALFLTLVLLAGLTACKTADAADPAQEQNTVTAPTNDATF
                     VYQPDDPTLIPAGGQTIALVTGPSGTENGADAMLWQGVQTFANTYGYTADTYAAAGNT
                     ADDAETALRAAAESGARLVVCQGEAAGEALYRIQDNYPDVHYLLFDDEPHNADYSAYK
                     TAELVHCVLFQEEQAGYLAGYAVVTEGYTALGFIGTREVPGIVRYATGFLQGAEAAAE
                     LQGEDVTLQTWFTDTDENSDAITQRMVDWYNNGTALIVVSGGNLYEGAASAVNQTGGK
                     AVTTDVDNTALSGRVLASAVKCYNAAVQRELYSFFTNGSWDTQSAGQTEKVGYTTGAV
                     ALEAGAPWRFDTFTQDDYRKLYEDLRTSVRKVDAYADLDTLPDTPNVTVNRTM"
     gene            6470..7606
                     /locus_tag="ACZPAX_03025"
     CDS             6470..7606
                     /locus_tag="ACZPAX_03025"
                     /inference="COORDINATES: protein motif:HMM:NF014647.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family lipoprotein"
                     /protein_id="HIR1112904.1"
                     /translation="MKKFLAFVMAASMALSLAACGGSSASTATSTTTEATSEAASAAS
                     TSGSKTDVAFVTDVGNIDDQSFNQYTWQGVQDFCSANNLSANYYRPTEDSDAARLEQM
                     DNAVNDGAKSIVVAGYLFGKAIAEAQEKYPDVQFLALDVSTSDLGDKTPTANTALITY
                     KEEQAGYLAGYAAVYDGYKELGFLGGMAVPAVIRYGYGFVQGADAAAKEIGATDVNIK
                     YWYSGGFAATDEVKNKMDGWYSEGTEVVFACGGPVCQSCDAAAQANGGKMIGVDVDQS
                     GQFDTVVTSAMKGLAESVNEALTDALNNGWKFSETYSGKETKLGAAENCVGLPMATSK
                     FTKFTQEQYDTMYAAIVDGSLVIDDSFDADVKPAVTTITVDYQS"
     gene            7826..>8268
                     /locus_tag="ACZPAX_03030"
     CDS             7826..>8268
                     /locus_tag="ACZPAX_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007488572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR1112905.1"
                     /translation="MLHITKEFPGIKANDDITLQLRKGEIHALLGENGAGKSTLMSVL
                     FGLYQPEQGTIKKNGKEVKINNPNDANALGIGMVHQHFKLVECFTVLDNIILGVEDTK
                     HGFLQKDEARKKVMALSEKYGLKVDPDALVSDISVGMQQRVEILK"
BASE COUNT         1833 a         2554 c         2341 g         1540 t
ORIGIN      
        1 gacatacata tattttgtgt ccagatcctc gccctcgcgc tcccacggca tatcgtcgtc
       61 gctgcagccg atgggcatac cgttgtagcc caaagacaaa atcttattct cggggctgac
      121 gatacaagcg ccgacctggc tgttgttgtc cttcgaccgc atggccgaaa gcagggcaat
      181 gcccataaaa tattcatccc agctgatgta atcctgccgt ttcataggta caccgtcctt
      241 ttggcttttt ctttattata ctgtgccgtg cggcgcggtg caagatggtt cgcgcaaata
      301 tttccgccgc gtggcctcgc cgccgcgcag gtgacgctcg gccttgtttt tctgcacgac
      361 ggcctgcacc tcggcccaca agccggggtt ctggcggcgc agccggtgct ggtccttcca
      421 gatcgtggat ttgctcacgc cgaacacggc tgccgccgcc cgcacggtcg cgccgctctc
      481 cgcgatatac cgtcccaccg ccgccgcgcg ctcctcaggg tcgcccttca tagcctgacc
      541 tcctcacata ggaatttctg tcaaatccta tgtgaaagag gggcggagta tgctatactg
      601 tttttacaaa ggggggaaac ccgatgacat ttgaacgtta cccggaattg gataccctgc
      661 tgctggcaaa tcccggcgca gtgcgggatt ataaacccga gtggggctgg gatcgctata
      721 tggtgggcgg caaaatgttc gccgcactgc tgactacggc agacgactgt gccccggaat
      781 accaaaacca tccgatgctg acggtgaaag tcgcaccgga ggagggaaac ttcctgcagc
      841 gacagtaccc ggatatcctg cctgggtatt acatgaacaa gcagcactgg atcagcgtgc
      901 ggctggatgg ggccgtgccg caggaagagg tcatgcgcct gtgcaaaagc gggtatgcac
      961 tgatttttgc caagctgaca aagaaaatgc agaaagaaat ttttggggaa taggcaaggc
     1021 ccgcgcagga aaacatccgg cgcggatctt gtttatacaa ttttatacac gcggcatccg
     1081 tcattttcgt accagatggg ataccgcgcg gcgtaccaac tctcgtctgc atcggaaaat
     1141 ttgccgtaga cggaggaatc aaacagcaca taccccacat cccacgctgc cagtgtgccc
     1201 tcgttgggat gctcgtacag cgctgccatg gcgcggtact cgtcggcgta atccatgccg
     1261 tggtaataga cataactgcc cgacccgcag agtatccgcc gcccggccag cgcaaatacc
     1321 ggcgtgacat ggctgtcgct tgtcaaaaac agcgcgtcgc tctcggcgtt cgcatcaata
     1381 tagtcggcca gtgcaatgtc attcacgctc caatgctggt agtcgatgaa aatctcccgc
     1441 ccgacggtca gcacactgcc gaacatggcc gcaaaacagc acgccgccag ccccaacgcc
     1501 cgccagggca gggcacggac cttggcaaac aaatcgacca acagctgcgc cgctaaaatg
     1561 caccccagca tatgccagac gtaaagcagc ttgttattgt cgtaattgtt gggctggaac
     1621 acaacgaact ccgccagcgc caaaattgcc agcccgccgc cgtagagcca ccgctgtttg
     1681 gacttggcgt ggataaacgc cggaaccagc agcagataca caagcccgat atttttgatg
     1741 taaaaccaga aataattgtc cttcagcgtg ccgttgtcct ggccgttgat ccagttgaag
     1801 tgcagccgca gcatgtgctg accgttcagg ctttgggcca gcacggtggg cagcatctgc
     1861 gccagccacg ccgcgccgca caccgccgcc agccccagcc agggcaaaag cgtctcccgc
     1921 cgtgcaccct gcgccagagt gtatacgccg cccacaaggc acaaaagcac cagcgccagc
     1981 gcactgtggg tatgcaaaag cggcaggggc agcgccagca gggccagcca tggccaaagg
     2041 cggcgctggc cctcatagca gaaccgccac agcaggtaca cggcgggaaa gagcacgcac
     2101 cagccgaaca gcgttgcccg ctgggggatg agcagatcga cgatggggtt gacccactcg
     2161 atgttttgct ccacaaaatt cgtcggtgtg gtgtagaacc cggtgaagat gcctgcaaac
     2221 ttttcagcac tgctgagaaa atacacaaag ccgaacccgc tgcccataaa gaacaggtaa
     2281 aacgcaaggc ttgccttgac ggcgctgccc gtcatccggt gggctagctg ccagaacata
     2341 ctgtacaccg aaaaaaatgc gggaagcatc ggcagaagat acgccgtgcg caggtccgtt
     2401 cccagcacca aaaatacgct ggagacagtc tcgcacaaga acggataccc gaaccggtgc
     2461 tcgccgccca ataggggata gcacggtaaa aattcaccac tctgcgcgat atatttaatg
     2521 aaccccaggt gcatcggcat atcgccgtag cagctctgcc cggtgtggta cgcgccgttt
     2581 accatgtgca gtacatgggt gtgtagcagg tacagcgtga tgagcgcgac cggcaggatg
     2641 caggcccaca gtgcgccgca attggcatct tttgccatgc cgcgcagccg cacaccccgg
     2701 cgcagcagca cgccgcccag aacgcctgcg gctgcagccg ccagcagaac ggcgggcagc
     2761 gtgaagccca gcgccagcgc aagcaccgca ggcagcaccg ccagcagcga cacgccaaac
     2821 ccgcagccca gcggcagaat aacagcagcg ctcccatccg gcaccgcccg gcgggccaag
     2881 agcaggccaa cgtatatgaa taaagcaaga tagagaatcg aaagcagcat ctttacactc
     2941 cttccaaatc aaacggcggc gtgtattcct cgcgggcgct gcttttttcc tgcatatagc
     3001 cgtcggtcag gcctaggcgc acggcctcgt caatgacctg ccggtactca taggtcgtga
     3061 tgcgccgccc taaaccgtgc tcggccgcct tataaaaagg ggtaaactgg ctcatcaaac
     3121 tcgggataaa tgcgacgccc gtttccgccc gcagtgcggc cagccgctgc aaaacggcct
     3181 tgctgtccgc cgcatggccg ggcagcgcca gatgccgcac aatgacaccc ttctgtaaat
     3241 agccgtccgc atcgtacacc ggcgcaccgg tctgcaaaag catctggcga agggcggcgt
     3301 cggccacggc agggtagttc tgcgcggcgg aaagctcggc agccagcgca ccgtccgcat
     3361 acttgtagtc cgccagccag atatccacat acccggccag cgccttgacg gcctccaccg
     3421 tctcataccc gccggtgttg taaacgactg gcaggcagaa tccggcggtc cttgcctgat
     3481 ccagcgccgc cgtgacccag ggcaggtatt gcgatgccgt gacgaggttc agatttttcg
     3541 ccccgccgtt ttgcagattc ataaaaatct cggccagccg ctctacggaa atctccttgc
     3601 caacctcacc ctggctgatg gggtagttct ggcagtagca gcaccgcagc gtacacccgc
     3661 tgaaaaacac cgtgccgctg ccggtctcgg cgctggggtc gccgcttaaa cacggctctt
     3721 cccagtggtg cagggcggcg cgggctaccc gcagggcatc gtcggccccg caaaggcccc
     3781 gctgcccggc ggcgcggtcg gccccgcagg cccgggggca aagggtgcaa tggtgcggca
     3841 gctgcataag aatccctctt tcacaaaaaa ctttcattta attttaccac aaactgcgaa
     3901 agtgtggtat actataccta caaataacct aaaataaagg agtgtgctct tatgtctacc
     3961 cctcataacc gcgccgaaaa gggcgatttt gccaagaccg tgctgatgcc cggcgacccg
     4021 ctgcgcgcta aattcattgc cgagactttc ctcgagaacc cgcgcgtcgt caccgatgtg
     4081 cgcggtatgc tcggctatac cggcacctac cagggccgcc ccatcagcgt gatgggcagc
     4141 ggtatgggta tgccgtccat cggtatttat tcttatgaac tgttcagcca gtacggcgtt
     4201 gagaacatca tccgcatcgg ttccgcaggc tcctacaccg acaaggccaa gctgtttgac
     4261 gttgtgctgg ctaccggtgc ttactccgag agcaactacg ccgtgaccca gagcggcgac
     4321 accgacgata tccagacccc cagcgccgag ctgaacgacg ccctgcgtgc cagcgccaag
     4381 gctgcggaca tccctctcat cgagggcaac atccattctt ccgacgtgtt ctaccgccag
     4441 cccagcgacg ccaagcccac ctactgggaa aaactgcgcg acgaaaaggg ctgcctggcc
     4501 gttgagatgg aaagcttcgc gctgttccac aacgcgaagg tgctgggcaa gcgcgcggcc
     4561 tgcctgctga ccatctccga cagctttgtc tcgccggaga tcaccaccgc cgaacagcgc
     4621 caaaccagct ttaccgccat gatgaaggtc gcgctgggcg tggacgcctt tttggagaag
     4681 taagatggaa aaagctgaga tccaggccct gatccgcgaa gcctttgagg gccagaagtt
     4741 tgcctatgtg ccgtactccc gcttccatgt gggcgcggcg ctgcgggcca gggatggccg
     4801 cgtgttcaag ggctgcaaca tcgagaacgc aggttacacc cccacgaact gcgccgagcg
     4861 cacggccctg ttcaaggcag tcagcgaggg ggtgcgcgag tttgacgcta tcgccattgt
     4921 gggcagcaaa gtgggggaga agaacaccct cgtcactggc ccctgcggtg tctgccgcca
     4981 ggcgctctat gagttcggcg ggccgaagct gaccgtcatt atggcgaaag acgagaacga
     5041 ttacatcgtg accaccctcg gcgacctgct gccctacggc ttcggcccgg cgaatctgga
     5101 gtgaaaattc tttggctccc ctttgggggg cggcttttca gaaaaccaaa ggagttccct
     5161 taacccatga agaaattctt agcactgttc ctgacgctgg tactgctggc agggctgacc
     5221 gcctgcaaaa cggctgacgc cgccgacccg gcgcaggaac agaataccgt gaccgccccc
     5281 accaacgacg caacgttcgt ctaccagccc gatgacccga cgctgatccc ggcaggcgga
     5341 cagaccattg cactggtgac aggcccctcc ggcacagaga acggggcaga cgccatgctg
     5401 tggcagggcg tgcagacctt cgccaacacg tacggctaca ccgccgatac ctatgccgcc
     5461 gcggggaata cggccgatga tgccgagact gccctgcgtg ccgccgccga gagcggcgca
     5521 aggcttgtcg tctgccaggg cgaggctgcg ggcgaagcac tctaccgcat ccaggacaac
     5581 taccccgatg tacactacct gctgttcgac gatgaacccc acaacgccga ttacagcgcc
     5641 tacaagactg ccgaactcgt ccactgcgtc ctcttccagg aagaacaggc cgggtatctg
     5701 gcgggctacg ccgtcgtgac cgaagggtac accgccctcg gcttcattgg cacgcgggag
     5761 gtgcccggca ttgtgcggta cgccaccggc tttttgcagg gggcagaggc cgccgcagaa
     5821 ttacagggcg aggacgtgac cttacagacc tggttcactg acaccgacga gaacagcgat
     5881 gccatcaccc agcgtatggt ggactggtac aataacggta cagcgctcat tgtggtcagc
     5941 ggcggcaacc tgtacgaggg tgcggcctcc gcagtcaacc agaccggcgg taaggctgtc
     6001 accaccgatg tggacaacac cgccctgagc ggccgcgtgc tggcaagcgc ggtcaagtgc
     6061 tataacgccg ccgtccagcg ggagctgtac agcttcttca cgaatggcag ctgggacacc
     6121 cagagcgcgg gccagaccga gaaggtcggc tacaccaccg gcgcggtcgc gctggaagcg
     6181 ggtgcgcctt ggcggtttga caccttcacc caggacgatt accgaaaact gtacgaggat
     6241 ctgcgtacca gcgtgcgcaa agtggatgcc tacgccgacc tggacaccct gcccgacacc
     6301 cccaatgtga cggtgaatcg gaccatgtaa accctttgtt atgcttttgt aattcttttt
     6361 gtcatctttt ttcattgaat tgccgccgca gatgcggtat actgaagata ctgcggcggg
     6421 gaactcgccc cgctgcgcaa ctgaaaaaca acctaaggag atcaaaacca tgaaaaagtt
     6481 tcttgctttt gtaatggctg ccagcatggc gctctctctg gctgcctgcg gcggttcttc
     6541 cgccagcacc gccacgagca ccacgacgga ggctacctct gaggccgctt ctgccgccag
     6601 cacttccggc agcaagaccg atgtcgcctt cgttaccgac gttggcaaca tcgatgacca
     6661 gtccttcaac cagtacacct ggcagggcgt gcaggacttc tgctctgcaa acaacctgag
     6721 cgccaactac tatcgtccca ctgaggactc tgacgctgcc cgtctggagc agatggacaa
     6781 cgctgtcaac gacggtgcta agtccatcgt cgttgccggc tacctgttcg gcaaagccat
     6841 cgccgaggct caggagaagt accccgatgt tcagttcctg gccctggacg tttccaccag
     6901 cgacctgggc gacaagaccc ccacggccaa caccgctctg atcacctata aggaagagca
     6961 ggctggttac ctggccggct acgccgctgt ttacgacggc tacaaggagc tgggcttcct
     7021 gggcggcatg gccgttcccg ctgttatccg ttacggctac ggcttcgtgc agggcgctga
     7081 cgctgctgcc aaggagatcg gtgctacgga tgtcaacatc aagtactggt actccggcgg
     7141 cttcgctgct accgatgaag tcaagaacaa gatggatggc tggtactctg agggtaccga
     7201 ggtcgtcttt gcctgcggcg gccctgtctg ccagagctgc gacgctgctg ctcaggccaa
     7261 cggcggcaag atgatcggcg ttgacgttga ccagtccggt cagttcgaca ccgttgtcac
     7321 ctccgccatg aagggtctgg ccgaatctgt caacgaggcc ctgaccgatg ccctgaacaa
     7381 cggctggaag ttcagtgaga cctactccgg caaggagacc aagctcggcg ctgccgagaa
     7441 ctgcgttggc ctgcccatgg cgaccagcaa gttcaccaag ttcacgcagg agcagtacga
     7501 caccatgtac gctgctatcg tggacggctc tctggtcatc gacgacagct tcgatgctga
     7561 tgtcaagccc gctgtcacca ccatcaccgt ggactaccag agctgatctg atacggcaga
     7621 tgctttaaag gcatgacttt tgaccaccct gcgcaccccg cgcagggtgg tttttgttgg
     7681 ttttctacaa attctgacga ctttttgcgc agattctctt gtgcacagca gagtttttct
     7741 gtataatata gagtaagaat ttgtgtacaa gcccgtacac attgaaaggg gcgtgcaacg
     7801 attggcagat aattatgtca tcgaaatgct gcacatcacc aaggaattcc ccggtatcaa
     7861 agccaacgac gatatcaccc tgcagctgcg caagggcgag atccatgccc tgctgggcga
     7921 aaacggcgcc ggtaaatcta ccttgatgag cgtgctcttt ggtctgtacc agccggagca
     7981 gggtacgatc aagaaaaacg gaaaagaagt caagatcaac aacccgaacg acgccaacgc
     8041 gctgggcata ggcatggtgc atcagcactt caaactggtc gagtgcttca cggtgctcga
     8101 taacatcatc ctcggcgtcg aggatacgaa gcacggcttt ttgcagaagg acgaggcccg
     8161 caaaaaggtc atggccctga gcgaaaagta cggcctgaag gtcgacccgg acgcgctggt
     8221 cagcgacatt tccgtcggta tgcagcagcg tgtcgagatc ctgaagat
//
LOCUS       DBMDSQ010000049         8191 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_2365_length_8191_cov_1.749197, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000049 DBMDSQ010000000
VERSION     DBMDSQ010000049.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 8191)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8191)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8191
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2365_length_8191_cov_1.749197"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            58..786
                     /locus_tag="ACZPAX_03035"
     CDS             58..786
                     /locus_tag="ACZPAX_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015536930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIR1112906.1"
                     /translation="MMTVKEVSRLTGVSVRTLQYYDQIGLLHPGTRTEAGYRLYDAAT
                     LETLQQILLFRELKFSLKEIAVILQNPHFDREKALEQQIELLTLQKRHLESLITLAEN
                     IRQTGGNAMDFTAFDTKKLDEYAAKAKQQWGATAAYQEYETKNAHETTQAQQENARAL
                     MALVAAFGHMQTKDPADPAVQAQVQKLQAFITEHYYTCTKEILHSLGQMYGAGGEFTA
                     NINAAGGPGAAEFARKAIERYCCG"
     gene            865..1341
                     /locus_tag="ACZPAX_03040"
     CDS             865..1341
                     /locus_tag="ACZPAX_03040"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013498424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HIR1112907.1"
                     /translation="MFRTMRRFKQELTREECLAVLRSQPRGVLSLLGENGYPYGLPID
                     YWYNETDGKLYFHGAKEGHKIDAIRACDKASFCVHDEGQRVAGEWPLHFCSVIVFGRI
                     APVTDAALTETACRRLAEKFTDDAAYIDAEIAAARDRVLCLALTPEHMTGKRIKES"
     gene            1351..2205
                     /locus_tag="ACZPAX_03045"
     CDS             1351..2205
                     /locus_tag="ACZPAX_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HIR1112908.1"
                     /translation="MKKTIDTAALVKEAAILTGGVAIIAAAVYFFLVPSHASVSSISG
                     LGIVLANFVPLPLSAITMILNVVLLIIGFFTCGREFGVKTVYTSVMLPVFIALFERLF
                     PDIGSLTDSQELDVLCYILVVSVGLSILFNRNASSGGLDIVAKIMNKYLHMELGRAMS
                     LSGMCVALSAALVYDKKTVVLSILGTYFNGLVLDHFIFDHNIKRRVCIITEKEEALRR
                     FILSDLHSGATVYESYGAYNMQKRREIITIVDKNEYQKLMNYMNQEDPQAFITVYTVS
                     DIRYRPKV"
     gene            2455..2528
                     /locus_tag="ACZPAX_03050"
     tRNA            2455..2528
                     /locus_tag="ACZPAX_03050"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2487..2489,aa:Gln,seq:ctg)
     gene            2564..2636
                     /locus_tag="ACZPAX_03055"
     tRNA            2564..2636
                     /locus_tag="ACZPAX_03055"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2597..2599,aa:Pro,seq:cgg)
     gene            complement(2747..3742)
                     /locus_tag="ACZPAX_03060"
     CDS             complement(2747..3742)
                     /locus_tag="ACZPAX_03060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1112909.1"
                     /translation="MSETTFRLARLGEDTAIIDFINENFDMRLPLLNRPELFHHYYAG
                     RSGIPQFAVAEENGQYVSAAGYILANTKRKPDVWVSVWVAAKGHNGVGLELMNALPGL
                     LNADVVACNNIRPNTCTFYQFLGWKAERLPHYYRLGRRRAYYLAKPLRYSLPPVQRDL
                     GLAKVADAADLISLGMPATPHTPRKDVWYMRRRYFHYPHFKYDVWAAQENGKLLAYVV
                     TRTVSAQDTGCAAVVRLVDFIGEDAVLPRLGAALDAILNHAGAEYMDCYNAGIPADVW
                     LAAGFTERIEGDGCIIPNYLTPPLHENTEYYYFTNKPENFVMFKADGDQDRPNLK"
     gene            complement(3735..4475)
                     /locus_tag="ACZPAX_03065"
     CDS             complement(3735..4475)
                     /locus_tag="ACZPAX_03065"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR1112910.1"
                     /translation="MSYTYLITGATSDVGRALIERLLTAAPDATVLAQGCGDLEKLAD
                     LCARFPGQVCPFDVDLSDRAKVDTFVQVLAASAPAPTHFIHLPALPVVNAKFKAFDQT
                     RFDRDLEIQVHSAVRICRAVLPAMAKAKFGRVLFIQTSYTIGCPPKNTAAYVMAKSAI
                     GGLVKSLAVEYARFGVTVNCVAPSMMETNFLKDTPDLIVQAAAEENPMGRNATPADVV
                     PAMAFLLSDEARFITGVTLPVTGGSAIV"
     gene            complement(4490..4906)
                     /locus_tag="ACZPAX_03070"
     CDS             complement(4490..4906)
                     /locus_tag="ACZPAX_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HIR1112911.1"
                     /translation="MNHYTLAEMTPGLTEEFTVTVTPEMMDAFRAITGDVSPIHIDAD
                     YAKGRGYPGRVVYGMLGASFFSTLAGVYLPGEHCLLHGVECKFAKPIFIGDTLTIKGT
                     VVNVSEIGSEAEIKAVITNQDGKRVTRGIIKAGLAK"
     gene            complement(4975..5214)
                     /locus_tag="ACZPAX_03075"
     CDS             complement(4975..5214)
                     /locus_tag="ACZPAX_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HIR1112912.1"
                     /translation="MTEQEILNAVQDIFRDNFDDDTLEITRSTCADDIEDWDSLEQIN
                     LLTAIEKKFNIKFKLADVRGLKDVGDLLDLVARMV"
     gene            complement(5284..5589)
                     /locus_tag="ACZPAX_03080"
     CDS             complement(5284..5589)
                     /locus_tag="ACZPAX_03080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1112913.1"
                     /translation="MQNRQIRRVYLSLLTAAAVLCIAAGMVWFLRGQPSPAPARYLLK
                     DSGGHVALYTADGTGPLAIYEDIYTHLLPESDVLALQQGVQVADEVELQRRLEDYGL"
     gene            complement(5727..5930)
                     /locus_tag="ACZPAX_03085"
     CDS             complement(5727..5930)
                     /locus_tag="ACZPAX_03085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1112914.1"
                     /translation="MKLKIMPMMLRRFFGFFFFFGFFGAGAPGWPVAPAVVFTPSVEI
                     WRCCLGSMGTASFGLYQSARQRP"
     gene            5914..>8191
                     /locus_tag="ACZPAX_03090"
     CDS             5914..>8191
                     /locus_tag="ACZPAX_03090"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013100.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HIR1112915.1"
                     /translation="MIFSFIGCMLCLCIMAASVGGVLLSMYIVQVTADDAETLDLDNQ
                     KNKQTTIIYDRYGDEYAQLTRGEDRIWRELSAMPDNLKNAVVAIEDKNFWTEPGINLK
                     GTIGAALNAFTGNRIWGTNRGASTLEQQLIKNLTGDNEQDNMRKVREIFRALGLDNKY
                     SKETVLEAYLNTIPLTGIVHGMEAGSLQYFGKHVEDLTLSECAVLASITKNPTKYNPA
                     TNPEELIKRRNHVLYEMQSQGYITEAEFEAAKAETITLVESSAATENATRSSSNSWFT
                     DALYTELLSQLQEDLNYTADEAKELIFSGGLRIYSTVDPTVQAGIEKTMYNEDDLIPA
                     LWHEEPVCLRDYPADSSSWDEVQYDEATGLPITKDGYAVYGQEAIPIYADEEGTTLKM
                     GTSTDPDYPNDTTVYLCVYEKVRTQAAMATLDYDGSILGIGGGIGEKKYDLGFNRATS
                     PHQIGSTMKPIGAYALALDYKLINYSSQILDSPYYSAEDKKVLKDQYIGVMSPYSEAA
                     QSRSDVWRAWPTNYGGAGGQGNPMLVYDALQQSYNTVAVWVGDMVGVDYLYNFVHDTL
                     ECSYINAENDMDLGPLVLGSQSSGLTVVQLAGAYTMFNTGTFTTPHYYTEITDYQGNM
                     ILDNNKYINTTQAISADTAYIMNRMMWNVLHSSKGTAYGKGPDGEMDSVAKTGTTSNY
                     KDYTFAGLTPYYVTAIWWGCDRPTEMDTLGKAGKNASPIQYAWKYLMEDLQADLPVKE
                     FAKGENVVEKRFDTSTGAI"
BASE COUNT         1830 a         2402 c         2386 g         1573 t
ORIGIN      
        1 tgaccctgac gctgcgtgat actgtatgtt gaaactaccg cgaaaggaga aagcgctatg
       61 atgactgtaa aagaggtcag ccgcctgacc ggtgtgagcg tgcgcaccct gcaatattac
      121 gaccaaatcg gcttgctgca ccccggcacc cgcaccgagg cgggctaccg cctctacgat
      181 gccgccaccc tggagacctt gcagcagatt ttgctgttcc gtgagttgaa attctcgtta
      241 aaagagatcg ccgtgatcct gcaaaacccg cacttcgacc gggaaaaggc gctggaacag
      301 caaatcgagc tgctgaccct ccaaaaacgg catctggaaa gcctcatcac tctcgcggaa
      361 aacatccgac aaacaggagg aaatgccatg gattttaccg catttgacac gaagaagctc
      421 gacgagtacg ccgcaaaggc caaacagcag tggggcgcaa cagcagccta ccaggagtac
      481 gagacaaaga acgcccatga gacaacacag gcccagcagg aaaacgcccg tgcgttgatg
      541 gccctggtcg cggcgttcgg ccacatgcag acgaaagacc cggctgaccc ggccgtgcag
      601 gcacaggtgc agaaattgca ggcgttcatc actgaacatt actacacctg caccaaagaa
      661 attttgcaca gcctgggcca gatgtacggt gcgggcggcg aatttaccgc gaacatcaac
      721 gcagcgggcg gccccggcgc ggcggagttc gcccggaaag ccatcgagcg atactgctgc
      781 gggtgagttc caaacttgcc cattgtatcc cgccgcgatt tgtgctagta tcaatagcag
      841 cagccaatag aggaggaaac cagtatgttc agaaccatgc gccgctttaa acaggagctt
      901 acccgggagg aatgcctggc cgttctgcgc agccagcccc gcggggtgct gtcactgctg
      961 ggggagaatg gctaccccta cggcctgccc atcgactact ggtacaatga gactgacgga
     1021 aagctctact tccacggtgc gaaggaagga cacaagattg acgccatccg cgcctgcgat
     1081 aaggcgtcct tctgcgtaca cgacgaagga cagcgcgtgg caggggagtg gccgctgcat
     1141 ttttgcagtg tcatcgtgtt tgggcgcatc gccccggtga cggatgccgc cctgactgaa
     1201 accgcctgcc gccgcctggc cgaaaagttc accgacgatg ccgcttatat cgacgccgag
     1261 atcgccgcgg ccagggaccg ggtgctctgc ttggccctga cgcctgagca tatgaccggc
     1321 aagcggatca aggagagttg ataggacacg atgaaaaaga caatcgacac tgcggctctg
     1381 gtaaaagaag ccgccatcct gaccgggggt gtagccatca ttgcggctgc cgtgtatttc
     1441 ttcctcgtgc caagccatgc gtcggtcagc agtatttcgg gcttgggcat tgtgctggca
     1501 aactttgtgc cgttgccact gtccgccatc acgatgattt tgaacgtcgt gctgctcatc
     1561 atcggctttt tcacctgcgg gcgggaattc ggtgtcaaga ccgtctacac cagcgtcatg
     1621 ctgccggtgt tcatcgccct gtttgagcgg cttttcccgg atatcggctc gctgaccgac
     1681 agccaggaac tggacgtgct gtgctatatc cttgtcgtca gcgtgggatt gagcatcctg
     1741 ttcaaccgca acgcatcgtc gggcgggctg gatatcgtgg caaagatcat gaacaagtac
     1801 ctgcatatgg agcttggccg cgccatgtcg ctgtccggta tgtgcgtggc gctctcggcg
     1861 gcgctggtct acgacaaaaa gaccgtggtg ttaagcattt taggcaccta tttcaatggc
     1921 cttgtgctgg accactttat ctttgaccac aacatcaagc gccgtgtctg catcatcacc
     1981 gaaaaagagg aagcgctgcg ccgcttcatc ctcagcgacc tgcacagcgg cgcgacggtg
     2041 tatgaatcct acggtgccta caatatgcag aagcggcggg aaatcatcac catcgtggac
     2101 aagaacgaat accagaaatt gatgaactat atgaaccagg aggatccgca ggccttcatc
     2161 acggtctaca ccgtgtcgga catacggtat cggccgaaag tgtgaaaccc catgcaaacc
     2221 caaaccgctg ccaaaccgca gcaggatatg accgcccagc tggccgaagg gcagatcttc
     2281 ccgcgtatct tccgcgagcg ggagacagaa gtttagcgca ggatcgcaaa atttttgaag
     2341 agataaaaaa gttcgcaaaa aaaggagagt tttttcaaaa aaagcgttga caaatcccac
     2401 cgggcacgct ataatatatg agcgttgagg gcagacgccc aaaacaaaac ccgatgggga
     2461 ttcgcataat ggtagtgcag cggactctga ctccgcccgt gggagttcga tcctctcatc
     2521 cccaaccaca ccgcagcagc ttagctgctg cggtttttct ttccggggtg tagcgcagtt
     2581 ggtagcgcgc ttggttcggg accaagaggc cgtgggttca aatcccgtca ctccgactga
     2641 aaaccgccag atatcgtttg atgtctggcg gtttttattt tgcatttaaa ataaaatcaa
     2701 aaacaccgat agcctttcca gccggaaaag ccatcggtgc atttttttac ttcaaattcg
     2761 gtcgatcttg gtcgccgtcc gctttgaaca tcacgaaatt ctcgggctta ttcgtgaaat
     2821 aataatactc ggtgttctcg tgcaggggcg gggtcaggta attggggatg atgcagccgt
     2881 cgccctcgat gcgctcggtg aagccggcgg caagccagac gtcggcgggg atgccggcgt
     2941 tgtagcagtc catgtactcg gcacccgcgt ggtttaaaat cgcgtccagc gcggcaccca
     3001 ggcggggcag cacggcgtcc tcaccgatga aatccaccag ccgtacgacg gcggcgcagc
     3061 cggtgtcctg ggcggacacc gtgcgggtca cgacgtaggc cagcagcttg ccgttctcct
     3121 gcgcggccca gacgtcatac ttgaagtgcg ggtagtggaa ataccgccgc cgcatatacc
     3181 acacatcctt gcggggcgtg tggggcgtgg cgggcatccc cagcgagatc aggtcggcgg
     3241 cgtccgccac cttggcaagg cctaaatcgc gctgcacagg gggcagtgaa taccgcagcg
     3301 gctttgccag gtagtaggcg cggcggcggc ccaggcggta gtagtggggc aggcgttcgg
     3361 ccttccagcc caaaaactgg taaaacgtgc aggtgttggg gcggatgttg ttgcaggcca
     3421 ccacgtcggc gttcagtagg ccgggcaggg cgttcatcaa ttccagcccg acgccgttgt
     3481 ggcccttggc ggccacccag acgctgaccc agacatcagg cttgcgcttg gtgttggcca
     3541 gtatgtaccc agcggcgctg acgtactggc cgttctcctc cgccacggca aactgcggga
     3601 tgccgctgcg gccggcatag tagtgatgga acagctcggg ccgattgagc agcggcagac
     3661 gcatatcaaa attttcgttg ataaagtcaa tgatggcggt atcctcaccc aggcgtgcca
     3721 gccggaaagt tgtctcagac aatggcagac cctcctgtga ccggcagcgt tacgccggtg
     3781 atgaaccgtg cctcgtcgga gagcaggaac gccatggcgg ggacaacatc ggcgggcgtc
     3841 gcgttgcggc ccatggggtt ttcttcggcg gcggcctgca cgatgaggtc aggggtatcc
     3901 tttaagaaat tcgtctccat catgctgggg gccacgcagt tgaccgtgac gccgaagcgg
     3961 gcgtactcga cggccaggct cttgaccagc ccgccgatgg cacttttcgc catgacgtag
     4021 gcggcggtgt tcttgggcgg gcagccgatg gtatagctgg tctggatgaa cagcacgcgg
     4081 ccaaacttgg ccttggccat ggcgggcagc acggcgcggc agatacgcac ggcgctgtgg
     4141 acctgaattt ccaaatcgcg gtcaaaacgg gtctggtcaa aggccttgaa cttggcgttg
     4201 acgaccggca gcgcgggcag gtggatgaaa tgcgtcggcg cgggggcaga cgccgccagc
     4261 acctgcacaa aggtatcgac tttggcgcgg tcggacaggt caacgtcaaa ggggcacacc
     4321 tggccgggaa agcgggcgca gaggtcggcc agcttttcca ggtcgccgca gccctgggcc
     4381 agcaccgtgg cgtcgggggc ggcagtcagc agccgctcga tcagtgcgcg gcccacatcg
     4441 ctggtagcgc cggtgataag ataggtataa gacatggttt ttctcctctt tatttcgcta
     4501 gcccggcctt gataatgccg cgggtcacgc gcttgccgtc ctggttggtg atgacggcct
     4561 tgatttcggc ctcgctgccg atctcactga cgttcacgac ggtgcccttg atggtcagcg
     4621 tgtcgccgat gaaaatgggt ttggcaaact tgcactccac gccgtgcagc aggcagtgct
     4681 cgccgggcag gtagacgccc gccagcgtgg agaaaaagct cgcccccagc atcccgtaga
     4741 cgacgcggcc ggggtagccg cggcccttgg cgtagtcagc gtcgatgtgg atgggggaga
     4801 cgtccccggt gatggcgcgg aaggcgtcca tcatttccgg cgtgacggtc acggtaaact
     4861 cctcggtcag gccgggggtc atttccgcca gcgtgtagtg gttcatggca ggctcctttg
     4921 gcaaaagcat ttgaaaaaag gcggcaggat gccgccccaa aacgatattg agatttacac
     4981 catccgtgca accaggtcca gcaggtcgcc cacgtccttc aggccgcgga cgtcggccag
     5041 cttgaacttg atgttgaact tcttctcgat ggcggtcagc aggttgatct gctccaggct
     5101 gtcccagtcc tcgatatcgt cggcgcaggt gctgcgggtg atctcaaggg tgtcatcgtc
     5161 aaaattatcg cggaaaatat cctgtaccgc gttcagaatt tcctgttcag tcatactgta
     5221 actcctttta tattatggta gttacctata ctatagcata aaagcagagc agacgcaaga
     5281 tttttacagc ccataatcct ctaaccgtct ctgcaattct acctcatctg ctacctgcac
     5341 gccctgctgc agggccagca cgtcgctttc gggcagaagg tgggtgtaaa tatcctcata
     5401 gatcgccagc gggccggtgc cgtcggcagt gtacagcgcc acatggccgc cgctgtcttt
     5461 taaaaggtag cgcgccgggg caggggaggg ctgcccgcgc agaaaccaca ccatccccgc
     5521 cgcgatgcaa agcaccgctg ctgccgtcag cagcgaaaga tacaccctgc ggatttgtct
     5581 gttttgcata ataattcccc cttgcgtgga tagtatgggc accattgcag acaaaaacac
     5641 cgaaagttgt gaattttacg tccgatactg tacttttggc cccaacttgt ggtataattc
     5701 attctataac attgtgcgat tctgtgttac ggcctttgcc gtgcgctttg atacagacca
     5761 aaggaggcgg ttcccataga acccaagcag cagcgccata tctccaccga gggcgtaaag
     5821 accaccgcag gtgccaccgg ccagcccggc gcacccgcac cgaaaaaacc gaaaaaaaag
     5881 aaaaaaccga aaaagcgccg cagcatcatc ggcatgatct tcagcttcat cggctgtatg
     5941 ctctgcctgt gcatcatggc ggccagcgtg ggcggcgtgc tgctgtccat gtatatcgtg
     6001 caggtcacgg cagatgacgc cgagaccctt gacctggaca accagaaaaa caagcagacg
     6061 acgatcatct acgaccgtta cggcgacgag tacgcccagc tgacccgcgg cgaggaccgc
     6121 atctggcgcg agctttccgc catgccggac aacctgaaaa acgccgtcgt cgccatcgag
     6181 gataagaact tctggaccga acccggcatc aacctcaagg gcaccatcgg tgccgcgctg
     6241 aacgcgttca ccggcaaccg catctggggc accaaccgcg gcgcgtccac gctggaacag
     6301 cagctcatta aaaacctgac cggcgataac gagcaggaca atatgcgtaa ggtgcgcgag
     6361 atcttccgtg ccttgggtct ggacaacaag tacagcaaag agaccgtcct tgaagcgtac
     6421 ctgaacacca tcccgctgac cggcattgtc cacggcatgg aggctggctc gctgcaatac
     6481 ttcggcaagc atgtcgagga cttgaccctg tcggagtgcg ccgtgctggc ctctatcacg
     6541 aagaacccga cgaagtacaa ccctgccacc aaccctgaag aactcatcaa gcgccgcaac
     6601 catgttctgt acgagatgca gtctcagggg tatatcaccg aggctgagtt tgaagccgcg
     6661 aaggccgaga ccatcacgct ggtggaaagc tccgccgcga ccgagaacgc gacccgttct
     6721 tccagcaact cctggttcac cgatgcgctc tacaccgagc tgctgagcca gctgcaggag
     6781 gatctgaact acaccgccga tgaggcgaag gaactgatct tctccggtgg tctgcgcatt
     6841 tactccaccg ttgaccccac cgtgcaggcg ggcatcgaga aaacgatgta caacgaggac
     6901 gacctgatcc ctgccctgtg gcatgaggag ccggtctgcc tgcgcgatta tcctgcggat
     6961 tccagcagtt gggacgaggt gcagtacgac gaggctaccg gtctgcccat cacgaaggac
     7021 ggctatgcgg tctacggcca agaggctatc cccatctacg ccgatgagga gggcacgacc
     7081 ctcaagatgg gcacctccac cgaccccgac taccccaacg acaccaccgt ctacctctgc
     7141 gtgtatgaga aagtccgcac ccaggctgca atggcaacgc tggactacga cggcagcatc
     7201 ctgggcatcg gcggcggcat tggcgagaag aagtacgacc tgggcttcaa ccgcgcgact
     7261 tctccccacc agatcggttc tacgatgaaa cccatcggcg cgtatgcgct ggcgctggac
     7321 tacaagctca tcaactattc ttctcagatt ctggattcgc cctactactc tgccgaggac
     7381 aagaaggtcc tgaaggatca gtatatcggc gtgatgagtc cctattccga ggcagcccag
     7441 tcccgcagcg atgtctggcg cgcctggccc accaactacg gcggtgcagg cggccagggc
     7501 aacccgatgc tcgtctatga cgccctgcag cagtcctaca acaccgtggc tgtctgggtc
     7561 ggcgatatgg tcggcgtgga ttacttgtac aactttgtgc atgacacgct ggaatgcagc
     7621 tatatcaacg ccgagaacga tatggacctc ggccccttgg tgctgggttc ccagagcagc
     7681 ggcctgacgg tcgtacagct ggccggtgcc tacacgatgt tcaacaccgg caccttcacc
     7741 acgccgcact actacaccga gatcaccgat taccagggca acatgatcct ggacaacaac
     7801 aagtatatca acaccaccca ggccatcagc gctgacacgg cctacatcat gaaccgcatg
     7861 atgtggaacg tcctgcacag cagcaagggt actgcctacg gcaagggtcc cgacggcgag
     7921 atggactccg tggccaagac cggtacgacc agcaactaca aggattacac cttcgcaggc
     7981 ctgacgccgt actacgtgac cgccatctgg tggggctgtg accgcccgac cgagatggac
     8041 acgctgggca aggcaggcaa aaacgcaagc cccatccagt acgcctggaa gtatctgatg
     8101 gaagatctgc aggccgacct gccggtcaag gagttcgcca aaggcgagaa cgttgtggag
     8161 aagcgctttg acacctccac cggcgcaatc g
//
LOCUS       DBMDSQ010000050         8056 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_2418_length_8056_cov_1.941923, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000050 DBMDSQ010000000
VERSION     DBMDSQ010000050.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 8056)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8056)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8056
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2418_length_8056_cov_1.941923"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1221
                     /locus_tag="ACZPAX_03095"
     CDS             <1..1221
                     /locus_tag="ACZPAX_03095"
                     /inference="COORDINATES: protein motif:HMM:NF014051.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HIR1112916.1"
                     /translation="GLYQLILALYMVFVSFATSGINVASARLAAQSLARGSGMAETLR
                     GLCFTALGFGTTAMVAQAVLAGPCARYLLHDIRAETALLILAPSLPFMAVSGAVRGCF
                     LAARRVQPNITAQLIEQLVRMAVAVAGLRALAQWGAGYGCAAVLLGNTVSEGVSCGVM
                     LAFAARTPEFVPRPGAPLHPYTRKELYDILWPIEGSRLLASALQAAESSLIPYTLALC
                     TGSRTEAVAQYGSLKGMALPLLFFPFSVLGALSGLLMPEITRAHTKGDTAAMRRLIFT
                     MLRLTGAFSLAAGAGFVVFGAPLAQFVYHDAEVGRYVQILGFVAPFMYLESMVDGVLK
                     GLGEQLATFRYSLLDSILRITAIWFVVPKYGMMGFLGVMVVSNLMTFCLNMKRMMEQI
                     KKPSPDRGRRPKAG"
     gene            complement(1323..3095)
                     /locus_tag="ACZPAX_03100"
     CDS             complement(1323..3095)
                     /locus_tag="ACZPAX_03100"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019189690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1112917.1"
                     /translation="MSETTTPQRPKNGALIRRFLPYMVKYRGVLAFDLFCAALTTLCD
                     IALPTIMRNLTNTVSAAALTVDAVLKMAALYFVLRVIDGAASYFMSGIGHIMGVHIET
                     DMRRDAFDHLLRLDHTYYNNTKVGQIMGRITNDLFDVTEFAHHCPEEFFIAGIKIVAS
                     FVILCQASVPLTLVVFACVPLMGVVSVKLNHKLRERFRQQRFQIGELNATIEDSLLGQ
                     RVVKAFAAEDLEREKFEQGNRDFENIKTLGYHAMAAFNTSTRLFDGLMYFVVILAGGL
                     SLVYGAISAGDLVAYVLYVSTLIATIRRIVEFAEQFQRGMTGIERFAEIMDTPIAIAD
                     APNAKDLEVKEGGIDFDDVSFEYPDDHNKVLRHVDLHILPGENVALVGPSGGGKTTLC
                     NLIPRFYDVTGGTIRIDGQDVRSVTLHSLRSAIGVVQQDVYLFSGTVAENIAYGRPGA
                     TRAEIEEAARLAGADAFVRALKDGYDTYVGERGVKLSGGQKQRLAIARVFLKNPRILL
                     LDEATSALDNESEILVGQSLDKLAKGRTTLTIAHRLTTIKNADRILVLGKDGIEEEGS
                     HEELLAKQGVYYRLWNGLVSGETL"
     gene            complement(3212..4993)
                     /locus_tag="ACZPAX_03105"
     CDS             complement(3212..4993)
                     /locus_tag="ACZPAX_03105"
                     /inference="COORDINATES: protein motif:HMM:NF024863.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HIR1112918.1"
                     /translation="MKLPFCRAALLAAALGVCLVGCKPAATDEPAASATAETAEVAAT
                     LTPTAATTAQPVAAEIGTVLPATEDAGRSYLDETLFIGDSNTVRYTMYADETGTAFAS
                     VDNSIGVVSMGAGAITTLKCEKFKGSSTMYTVPEAVAMLKPQRIIICYGTNNLGGSST
                     DATSYIKTYLQGLQAIQTAWPYCDIIVSAIPPLDKQRENTNLTMTQVDAYNAALVTMC
                     EENGFKFLNSAEVLRDAATGWAKTDYTLSDGVHLSKTAVTAYFDYVRTHAYITEDRRP
                     QPLGSIPQPDGVPANLITSDPIAVRGAKVPLEFVATNGGKVSGSTSQLVKKGGTASAV
                     TAVADEGFVFAGWTASSGGSYSSATISFTMPQNADAGGVVLTAHFKADAHEHEYVEVE
                     GTKTAATCLKAGSAKYKCKICGEVVEKDIKPLGHDWDDGVQKGAEIIYTCKREGCGET
                     KVEAAKATPSPSPSPTPVVTTAPTAVPTQAPTPVPTAVPTAAPTAVPATPEPTAVPTP
                     TPEPHTHDFQLISDTTTCTAAGTKTYQCSCGETKTEESAALGHSGVEQPDGSVVCSRC
                     NEVLRAAPAPEPVTPSEPTTESDTPTE"
     gene            5231..5689
                     /locus_tag="ACZPAX_03110"
     CDS             5231..5689
                     /locus_tag="ACZPAX_03110"
                     /inference="COORDINATES: protein motif:HMM:NF022636.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HIR1112919.1"
                     /translation="MNYHWNDRKSITLTRVVVATAILGSAIMTISGPWLTRWMVTAHA
                     LPPRTGPVLLVLGYLCAVLAFGMLFSLYKFLRRIEAGQIFVPANVTALRRISWCCVGA
                     AVLCLPAGVGIYLPFAFLGVAAAFMALIVRVLKNAFEQAVRMKDELDLTI"
     gene            5702..5917
                     /locus_tag="ACZPAX_03115"
     CDS             5702..5917
                     /locus_tag="ACZPAX_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020816673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1112920.1"
                     /translation="MPIVVNLDVMMARRHKGAGELAEEIGITPANLSILKNNKAKAVR
                     FSTLEALCKALDCQPGDILEFVPDENR"
     gene            6102..7328
                     /locus_tag="ACZPAX_03120"
     CDS             6102..7328
                     /locus_tag="ACZPAX_03120"
                     /inference="COORDINATES: protein motif:HMM:NF025067.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HIR1112921.1"
                     /translation="MPVFAALFIVYVDCAARAAHRTAARETPIWAGCWLALSLTYPLF
                     GYQPDPLGTWQWMAWHIFAVWYVLARCGMLAQGYSGSLVLLDGLAGVFRLPFGNFFLR
                     VRTVFSALKTGLHGRIKARKALRVLATAALTLALCGTAWGLLAAADANFAALGQSVAD
                     WWNSLLNNIKYIDTLMYILLSLPVGAWLYGLVFGALRRTEPPTTAAQCTAALEHARIV
                     PRSTATVAVAALCGVYALFFAVQAGEWFAAAPLGLSAPDAAAFAVDGFWELQKILLLN
                     FGVLAGVHFLGRAPLPKALAAVFCGFGLAFAALAAGKLAVYVVLYGLTPRRVIAGWFL
                     GVLAVWCVLALVRVFRAIPAAHIAILVLAVSFTVLACVNMKQRIINANLARVEAGIDE
                     EPDWGVLWECGYREEP"
     gene            complement(7537..>8056)
                     /locus_tag="ACZPAX_03125"
     CDS             complement(7537..>8056)
                     /locus_tag="ACZPAX_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HIR1112922.1"
                     /translation="DESVGILAETDGTPHNTVTPILHYDETDGFILDLALRNNRTSAE
                     FPDGIFHPHKEYHNIKKENIGLIEVMGLAILPARLKDQGAQIAEILTGQRPNTSREPG
                     DTLAVHADWIDELIAKYGTHMKPEAANKAVKAEIGTVFSHVLENAGVFKQDAQGQAAF
                     VRFMQSVGFKKV"
BASE COUNT         1341 a         2369 c         2636 g         1710 t
ORIGIN      
        1 ggcctgtacc agctgattct ggcgctgtac atggtgtttg tgtccttcgc cacgtcgggc
       61 atcaacgtgg cgtcggcgcg gctggcggcc caaagcctgg cccggggcag cggcatggct
      121 gagacgctgc gtgggctgtg cttcacggca ctgggcttcg gcacaacagc catggtggcg
      181 caggccgtgc tggccgggcc gtgcgcacgg tacctgctgc acgatatccg cgccgagacc
      241 gcgctgctga ttttggcccc cagcctgccg tttatggcgg tgtccggagc ggtgcggggg
      301 tgctttttgg ctgcgcggcg ggtgcagccc aatatcacag cgcagctcat tgagcagctg
      361 gtgcggatgg ctgttgccgt ggcggggctg cgggcgctgg cccagtgggg cgcggggtac
      421 ggctgcgccg ccgtgctgct gggcaacacg gtcagcgagg gcgtttcctg cggcgtcatg
      481 ctggcgtttg ccgcccgcac gccggaattt gtgcctcgtc caggcgcccc gctgcacccc
      541 tacacccgca aggaactcta cgatattctt tggccgatcg agggcagccg cctgttggcc
      601 agcgccctgc aggcggccga gagcagtctg attccctaca cgctggcgct ctgcaccggc
      661 agccgcaccg aggccgtggc ccagtacggc agcctgaaag ggatggcgct gccgctgctg
      721 ttctttccgt tttcggtgct gggggcgctg tccggtctgc tgatgccgga aatcacccgc
      781 gcccacacca agggagacac cgccgccatg cgccgcttga ttttcacgat gctgcgtctg
      841 accggcgcat tctcgctggc ggcaggggcc ggatttgtgg tgttcggtgc gccgctggcg
      901 cagttcgtct accacgacgc cgaggtcgga cggtatgtgc agattttagg ctttgtcgcg
      961 ccgttcatgt acctggaaag catggtggac ggcgtgctga agggcctggg ggagcagctg
     1021 gcgacgttcc gctattcgct gctggactcg atattgcgca tcacagccat ctggtttgtt
     1081 gtgccgaaat atggcatgat ggggttcctg ggggtgatgg tggtgtcgaa tttgatgacg
     1141 ttctgcttga atatgaagag gatgatggag caaataaaaa agccttcccc cgatagggga
     1201 aggcggccga aggccggatg agggacggac ttgccatgat tgcccggtaa ggggcggcgg
     1261 gggtaaatct cccatcatca gcccgcttcg cgagcagctt ctcccccaag ggagaagccg
     1321 ctttacaagg tttccccgct caccagtcca ttccacaacc tataatatac gccctgcttc
     1381 gccagcagct cttcatggct gccttcttcc tcaatgccgt ccttgcccaa aacgaggatg
     1441 cggtcggcgt tcttgatggt cgtcaggcgg tgggcaatcg tcagcgttgt gcggcccttc
     1501 gccagtttgt ccaggctctg gccgaccaaa atctcgctct cgttgtccag ggcgctcgtg
     1561 gcctcgtcca acagcagaat gcgcgggttc ttcaaaaata cgcgggcaat ggcgaggcgc
     1621 tgcttctgcc cgccggagag cttgacaccg cgctcaccga cataggtgtc atagccgtcc
     1681 ttcaaggcgc ggacaaaggc gtcagcccca gccaggcggg cggcttcctc aatttcggcg
     1741 cgggtcgcgc ccggacggcc gtaggcgata ttctccgcca ctgtgccgct gaaaagatac
     1801 acgtcctgct gcaccacgcc gatggcactg cgcaggctgt gcagcgttac gctgcgcaca
     1861 tcctggccgt caatgcggat ggtgccgcca gtcacatcgt agaagcgggg aatcaggttg
     1921 cacagcgtcg ttttgccgcc gccggacggc ccgaccagcg cgacattctc gcccgggagg
     1981 atgtgcagat ccacgtggcg cagcacttta ttgtggtcgt cggggtactc aaagctcaca
     2041 tcgtcaaagt cgatgccgcc ctctttgact tccaagtcct tcgcgttggg ggcgtcggca
     2101 atcgcgatgg gggtgtccat gatttccgca aagcgctcga tgccggtcat gccgcgctgg
     2161 aactgctcgg caaattcgac gatgcggcgg atcgtggcga tcagcgtgga cacgtacagc
     2221 acgtaggcaa ccagatcgcc cgcgctgatg gccccataca caagggacag accgcccgcc
     2281 aggatgacca caaagtacat caggccgtca aaaaggcggg tggacgtgtt gaacgcggcc
     2341 atggcatggt agcccagcgt cttgatgttc tcaaaatcac ggttgccctg ctcaaacttt
     2401 tcacgctcca aatcttccgc cgcaaacgcc ttgacgactc gctggcccag caggctgtcc
     2461 tcaatcgtgg cgttcagttc accaatctgg aatcgctgct ggcggaaccg ctcccgcagt
     2521 ttgtggttga gtttgaccga cacaacgccc atcagcggca cacaggcaaa caccaccagc
     2581 gtcagcggca cactggcctg gcagaggata acgaacgatg ccacgatctt tatgcccgca
     2641 atgaaaaatt cctcggggca gtggtgggca aactcggtca cgtcaaacag atcgttggtg
     2701 atgcggccca taatctggcc gaccttcgtg ttattgtagt aagtgtggtc cagccgcagc
     2761 aggtggtcaa aggcgtcgcg gcgcatatcc gtctcgatat gtacgcccat gatgtggccg
     2821 atgccggaca taaagtagct ggccgcgccg tcgatgacac gcaatacgaa gtagagcgcc
     2881 gccattttca gcacggcgtc cacggtaagg gccgcagcgc tgacggtatt tgtcaggttg
     2941 cgcatgatgg tgggcagcgc aatatcgcag agcgttgtca gcgcggcgca gaacagatca
     3001 aacgccagca cgccgcggta tttgaccata tacggaagaa aacggcgtat cagcgccccg
     3061 tttttcgggc gctgcggggt ggtagtttca gacatggatt cctctttcat tcggacttgt
     3121 aggggccggg catgcccggt ccgcacgtgg aaggcaaacg gtcgtttacc ggaaagctgc
     3181 gggccgcata tgtgcggccc ctacatggtg gttactcggt gggggtgtca ctttccgttg
     3241 ttggttcact cggcgtaact ggttctggcg caggtgcggc ccgcaaaacc tcgttacagc
     3301 gggagcacac aacgctgccg tcgggctgct caacgccgct gtgccccagt gcggcagatt
     3361 cctccgtttt tgtctcgccg cagctgcatt ggtaggtttt tgtgccggct gccgtgcagg
     3421 tggtggtgtc actgatcagc tggaaatcat gggtgtgcgg ttccggtgtc ggtgtcggca
     3481 ctgccgttgg ctcgggtgtg gcgggcacgg cggtcggcgc agcggtgggt actgccgtcg
     3541 gcaccggcgt cggtgcctgg gtgggcactg ccgttggtgc cgtcgtcacg accggcgtag
     3601 gggaggggga gggagacggc gtggccttgg cggcctcgac cttcgtctcg ccgcagccct
     3661 cgcgcttgca ggtgtagatg atttccgcgc ccttctgcac cccgtcatcc cagtcgtggc
     3721 ccagcggctt aatgtccttc tcgacgactt cgccgcagat tttgcacttg tacttggcgg
     3781 acccggcctt caggcaggtg gcggcagtct tggtgccctc gacctcaaca tactcgtgct
     3841 cgtgggcgtc ggccttgaag tgcgccgtca gcaccacgcc gcccgcgtcg gcgttctggg
     3901 gcattgtaaa gctgatggtc gcgctgctgt aactgccgcc ggagctggcc gtccagcccg
     3961 caaagacaaa gccctcgtca gccacggcgg tcacggcgct ggcggtgccg cctttcttga
     4021 ccagctggct ggtggagccg ctgaccttgc cgccgtttgt agcgacaaat tccagcggga
     4081 ccttcgcgcc gcgcacggct atcgggtcgc tggtgataag gttcgcaggg acgccgtcgg
     4141 gctgggggat ggaccccaac ggctgcgggc ggcggtcctc ggtgatatag gcgtgggtgc
     4201 gcacatagtc aaagtacgct gtaacggcgg tttttgacag gtgcacgccg tcggacagcg
     4261 tgtaatccgt cttggcccag ccggtggcgg cgtcgcgcag gacttcggcg ctgttcaaaa
     4321 acttaaagcc gttttcctcg cacattgtca ccagtgcggc attgtaggcg tccacctggg
     4381 tcatggtcag gttggtgttc tcgcgctgtt tgtccagcgg cgggatggca ctgacgatga
     4441 tatcgcagta gggccacgca gtctggatgg cctgcaagcc ctgcaaatag gtcttgatgt
     4501 agctggtcgc gtcggtggac gagccgccca ggttgttggt gccgtaacag atgatgatgc
     4561 gctggggctt gagcatcgcc acggcctccg gcacggtgta cattgtggag gaacccttga
     4621 atttttcaca tttcagcgtg gtaatagcac cagcgcccat gctgacgaca ccgatgctgt
     4681 tgtccaccga tgcaaaggcc gtgccggttt cgtcggcgta catcgtgtag cgcacggtgt
     4741 ttgagtcgcc gataaacagc gtctcgtcca ggtagctgcg gcccgcgtcc tcagtggcgg
     4801 gcagcacggt cccgatttcg gcggcgacag gctgtgcggt cgtcgcggcc gtgggcgtca
     4861 gcgtggcagc gacctcggct gtttcggctg tggcggaggc agcaggctca tcggtggcag
     4921 cgggcttgca gcccaccagg cagacgccca gcgccgcagc cagcagcgcg gcccggcaaa
     4981 agggtaattt catgcggtgc acccctcttt tttgtttcga tacacagcta tgatacaaca
     5041 ttttgtgtca atacgcaaat gaatcttgca ggttttacaa attcatcgaa aaacaataaa
     5101 aaatgcttgt aaaacgatat gcgccgtggt atactgcaaa caatgaaagc gggggcaggc
     5161 cacatgcagg gggcgatgcc tgcatccgtc cctacaataa caaccatttg cagcaacagg
     5221 agagctacct atgaactacc attggaacga ccgcaaaagt atcacgctga cccgggtggt
     5281 cgtggcgacg gccattttgg gcagcgccat tatgacaata tccggcccat ggctgacccg
     5341 ctggatggtc acggcccacg cgctgccgcc gcgcacaggg ccggtgttgc tggtgttggg
     5401 ctacctgtgc gcggtgctgg cgttcgggat gctgttcagc ctgtacaaat tcctgcgccg
     5461 catcgaggcc gggcagattt ttgtgcccgc caacgtcaca gcgctgcggc gcatcagctg
     5521 gtgctgcgtg ggcgcagcgg tgctctgcct gcctgcgggg gtgggcatct accttccgtt
     5581 tgcgtttttg ggcgtggcag cggcgtttat ggcgctcatc gtccgggtgc tgaaaaacgc
     5641 ttttgaacag gccgtccgca tgaaggacga gctggatctg acgatttaag ggggcggtgc
     5701 tatgcctatc gtggtcaatt tggatgtcat gatggcccgt cgtcacaaag gtgcgggcga
     5761 gctggctgag gaaatcggca tcaccccggc aaatttgtcg attttgaaaa acaacaaggc
     5821 caaggccgtg cggttttcga ccttggaagc cctctgcaag gcgctggact gccagccggg
     5881 ggatattttg gagtttgtgc cggacgagaa tcggtagggg ccgggcatgc ccggcccgcg
     5941 gcttacccgc aaacgcccac gtaattttac acgaccgggc cggacatatc cggcccctac
     6001 gggaaggtgg tttcgctatg ctgtatgcac ttttgatgta cccgctggcg tatttttatc
     6061 tgcgcggagt gatgtttccg cagtcatatt cggattgggg aatgccggtt tttgcggcgc
     6121 tgttcattgt gtatgtggac tgtgccgccc gggcggccca ccgcaccgcc gcccgggaaa
     6181 ccccgatctg ggccgggtgc tggctggcgc tgtcgctgac gtacccgctg ttcggctatc
     6241 agccggaccc gctgggcacg tggcagtgga tggcgtggca cattttcgcc gtctggtacg
     6301 tgctggcccg ctgcggaatg ctggcccagg ggtacagcgg cagcctggtt ttgctggatg
     6361 gcctggccgg ggtattccgg ctgcccttcg gcaatttctt cctgcgggtg cgcacggtgt
     6421 tttctgcgct gaaaaccggc ctgcatggcc gcatcaaagc ccgtaaggcg ctgcgcgtgc
     6481 tggctacggc ggcactgacg ctggcgctct gcggtacggc gtggggcttg ctggccgccg
     6541 ccgatgccaa ctttgcggcg ctgggccaga gtgtggccga ctggtggaac agcctgctca
     6601 acaatataaa atatatcgat acactgatgt atattctact gtcgctgccg gtgggcgcgt
     6661 ggctgtacgg actggtgttc ggcgcactgc gccgcaccga accgcccacg acggcggccc
     6721 aatgtaccgc tgcgctggaa cacgcccgca ttgtgccgcg cagtacggca acggtagcgg
     6781 tagccgcgct ctgcggtgtt tacgcgctgt tcttcgccgt gcaggcgggg gagtggttcg
     6841 ccgccgcgcc cctggggtta agcgctccgg acgccgctgc ctttgcggtg gacggcttct
     6901 gggagctgca aaaaatcctg ctgctcaact tcggcgtgct ggccggcgtg cattttctgg
     6961 gccgcgcacc gctgcccaaa gcgctggccg cggtgttctg cggcttcggg ctggcgtttg
     7021 ctgcgctggc ggcgggcaag ctggcggtct atgtcgtcct ctacggcctg acgccccggc
     7081 gggtcatcgc ggggtggttc ctcggcgtgt tggcggtctg gtgcgtgctg gcgctggtgc
     7141 gggtgttccg cgccatcccg gcggcgcata tcgccattct ggtgctggcg gtcagcttta
     7201 cggttctggc ctgcgtgaac atgaaacaac gcattatcaa tgcaaatttg gcccgcgtgg
     7261 aagccggaat cgatgaagaa cccgattggg gcgtgctgtg ggagtgcggg tacagggagg
     7321 aaccgtaggg gagggattca tccctcccgg gtcgtgtgcg gcggatttca cggtaaacat
     7381 cattgagttg taggggccgc acatgtgcgg cccgcgcgtg tacggcagac gactgcctgc
     7441 cggaaggtcg cgggccgggc atgcccggcc cctacaaggc aacgaaaaca gggacaccag
     7501 cccttccggg tcgatgtccc tgtttcttat gcgattttac actttcttaa accctacgct
     7561 ctgcataaac cgcacaaatg cggcctgacc ctgggcgtcc tgcttgaata caccggcgtt
     7621 ctccaaaacg tggctgaaca ccgtgccgat ctccgccttc acggccttgt tggcggcttc
     7681 gggcttcatg tgggtgccgt acttggcaat cagctcgtcg atccaatcgg cgtgtacggc
     7741 cagggtgtcg cccggctcac ggctcgtgtt cgggcgctgg cccgtcagaa tctccgcaat
     7801 ctgcgcaccc tgatccttca ggcgggcggg caaaatcgcc aggcccatga cctcgatcag
     7861 gccgatgttt tcctttttaa tattgtggta ttccttgtgc gggtggaaga taccatccgg
     7921 gaactccgca ctggtgcggt tgttgcgcag ggccaggtcc aggatgaacc catccgtctc
     7981 gtcgtagtgc aggatcggcg tcacggtgtt gtggggcgtg ccgtctgttt ctgccagaat
     8041 accgacgctc tcgtcg
//
LOCUS       DBMDSQ010000051         8013 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_2431_length_8013_cov_1.496208, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000051 DBMDSQ010000000
VERSION     DBMDSQ010000051.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 8013)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8013)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8013
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2431_length_8013_cov_1.496208"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..209
                     /locus_tag="ACZPAX_03130"
     CDS             <1..209
                     /locus_tag="ACZPAX_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HIR1112923.1"
                     /translation="SKVAGYANTFIFPNINAGNIGYKIAQRLGGFEAYGPILQGLNAP
                     INDLSRGCNAEEVYKMALITAALI"
     gene            complement(379..840)
                     /locus_tag="ACZPAX_03135"
     CDS             complement(379..840)
                     /locus_tag="ACZPAX_03135"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015564242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0043768 - S-ribosylhomocysteine lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HIR1112924.1"
                     /translation="MERIASFCVDHTKLLPGMYLSRQDGENGEILTWDIRMKQPNKGS
                     YLSPAAAHTLEHLFATYARNSKYSKGIVYVGPMGCLTGFYLLTIGLTPAEALDLTRSA
                     FAWMAAFEGDIPGASAVECGNYLMMDPIAARAEASAMLPVLENLDADKLHY"
     gene            complement(850..2595)
                     /gene="pyk"
                     /locus_tag="ACZPAX_03140"
     CDS             complement(850..2595)
                     /gene="pyk"
                     /locus_tag="ACZPAX_03140"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015564243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HIR1112925.1"
                     /translation="MRKTKIVCTMGPSTDKPGILRQLMENGMNVARFNFSHGDYEEHK
                     GRFDKVRALSKELDLPIACMLDTKGPEIRLGEFKNGVEKLVTGQKFTLTSRNVEGTNE
                     ICSVTYKDLPRDVKAGGRIMLDDGLIELRIDEVGDTDINCTVCNDGTIKTKKGVNVPG
                     VHLTMPYMSQRDTSDILFGIEQGFDLISASFARNAQDIMEIRHILDEHNSKIRIIAKI
                     ENQEGIDNIDEILTVADGIMVARGDMGVEIDFAEIPAIQKHLIDRAMSAGKICITATQ
                     MLDSMIVNPRPTRAEITDVANAIYDGTGAVMLSGETAAGKYPVEALKAMATIAETTEA
                     DSNFDSLVHHSGSDSTRLNVSAAVGHAACTTAADIGASAIITASKSGETARLLSRFRP
                     DTQIIACVLDETTRRQLNVYRGVTPLMMEYATSTDELISMSVAKAQEAGLVQDGDLVV
                     VTAGVPVGVSGTTNMIKVHMVGDSLLAGVGIGQYNAKGEVCVCRSAAEAAKKFKPGQI
                     LVVPFTTNDTLPFMREATGIITEEAGTNSHSAIVGLTLGKAVIVGATNATRTLKDGMV
                     ISMDCARGVVQAMAK"
     gene            complement(2749..2985)
                     /locus_tag="ACZPAX_03145"
     CDS             complement(2749..2985)
                     /locus_tag="ACZPAX_03145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1112926.1"
                     /translation="MQVQKFSTLPYTRPDYAAAQQKLRELTEQAEHAADYAALKAVVK
                     ERNALTAIGQPAYLLPTRQRQSCRRICTDEAEFV"
     gene            3155..4330
                     /locus_tag="ACZPAX_03150"
     CDS             3155..4330
                     /locus_tag="ACZPAX_03150"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - metabolic compound salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HIR1112927.1"
                     /translation="MAKRVFLIVLDSFGVGAEPDAPLFGDPGTNTLGAIAGHPNFKGD
                     NLAKLGMFNLDGVTCGTPVSAPIGSYARLREASAGKDTTIGHWEIAGLLSAEPLPTFP
                     NGFPQELLDEFTAKTGYKVLCNKPYSGTEVIRDYGEEHMKTGALIVYTSADSVFQIAA
                     NEALVPIEKLYEICTQARELLTGKYGVGRVIARPFVGDCAANFTRTPRRHDYSLVPPH
                     DTMLDAIQAAGKATIGVGKIHDIFAGKGIGETIRTSGNTEGLQVTLDLADRDFEGLAF
                     VNLVDFDMLYGHRRDIAGYAAAAAEFNDWLPGFMAKMCPGDILMVTADHGCDPSYTKT
                     TDHTREYVPFLIYGDEVKPGVNLHTRYSFATIADTVCKALNVDYCPAGCGVYDEIKK"
     gene            4314..5147
                     /locus_tag="ACZPAX_03155"
     CDS             4314..5147
                     /locus_tag="ACZPAX_03155"
                     /inference="COORDINATES: protein motif:HMM:NF014724.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HIR1112928.1"
                     /translation="MRSKSNVGLFLLYLLAAALVVYSVSLVILSNFNMGNLMVWLLTA
                     CVVGYAVFRRPLHAWFSHGTGRVVFWVLAALAAIYLAVIAFVSVSGYTNPPTGNEQVV
                     LVLGAGLHKDKPSKLLQYRLNKAYEFAAAHPDTLVITSGGQGRDEWCPEGDAMRDYLI
                     AKGLEPARVISEGRSTSTEENFAFSLAILRQHGYDETTPIVYVSNAFHCYRAGKYAAM
                     AGFTNATELAAGTPLRSVLPCYLREALALLYYWVFKTSVTGPMHAMVGLLDMNKKFFY
                     K"
     gene            5160..5921
                     /locus_tag="ACZPAX_03160"
     CDS             5160..5921
                     /locus_tag="ACZPAX_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015565703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HIR1112929.1"
                     /translation="MQIRYYKEYSRFLNRFMEFKVYGHAGRPIVIFPCQSGRFFDWED
                     RNMCNAAAPWIDNGKLQIFAVDSIDPESWDNHGPERPRIEMQERWYNYICEEFIPRLL
                     EINREQGEDHTGRILTGGASMGGGHAVNFYLRRPDIFNGTIALSGLYSSGMFFGNYMD
                     DLVYRNSPCDYMRNFPTTHPYMKLFEKADKFIMCCGQGAWEADLLASTRELQAILESK
                     GIHPIVDIWGQDVSHDWYWWEKQWVYFLQMTLGDA"
     gene            5940..7445
                     /locus_tag="ACZPAX_03165"
     CDS             5940..7445
                     /locus_tag="ACZPAX_03165"
                     /inference="COORDINATES: protein motif:HMM:NF039639.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
                     /protein_id="HIR1112930.1"
                     /translation="MDFWHDAAAQKRWLRRFVLLTAVLLVPVFALAVFARPSADDYIY
                     AARTHAVVQQYGFDLPRLLRAAWDTNAYYYENWQGLYVSGFTLAFQPAIFGNKWYGIT
                     LLCVLLPLFFCLYGLMRCVVLRLDPAQKHLPWALALLLTFAFIQGMPSPVEGLYWFNG
                     AMNYLPYFSLAALNAGLAFALCFAGKLVPRQKVLYALTGCVCSLVIGGGHQVAGLLNV
                     LVLLLAAVLCARRRNFWQLPAFFAAVLGLLLNVLAPGTRVRTAGFAGAGFAEAIVKSF
                     ILAAMQWLRWLDVPLLCLLALLALPLLQLARSAVLPDRVFRRPWLGAAVTFVLMWAMI
                     FLPSYTMGGIGAGRLLNVVWMTFVLGLAATEFLLLGWLERVRGVSLHRAERFCQHHAR
                     RLPLAVACMLVVMACIGSHTVKEGQDNYFATSLEAAYELADGQAQRYAAALDEREAIL
                     TDEAQPEVSIRPLNENERPWLLFYTDVAPGPDLWGLTPYFTKQSVTILDFE"
     gene            7561..>8013
                     /locus_tag="ACZPAX_03170"
     CDS             7561..>8013
                     /locus_tag="ACZPAX_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009256718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HIR1112931.1"
                     /translation="MIYLDYSANTPADPAVLDAYVAAERRYIANPNSTHIAGQEARAE
                     MERVTTSIANHLGVQPAEIIYTSGASEANNLAIKGIAQASRHIGKHIISTQLEHSSIG
                     ASLSVLQQQGYEIDLLNINRDGRVDLDQLRELMRNDTILVAVCAVDSEL"
BASE COUNT         1530 a         2329 c         2352 g         1802 t
ORIGIN      
        1 gctccaaggt tgccggttac gccaatacct tcatcttccc caacatcaat gccggcaaca
       61 tcggctacaa gattgcccag cgtctgggcg gctttgaggc ctacggcccc atcctgcagg
      121 gcctgaacgc ccccatcaat gatctgtctc gcggctgcaa cgccgaggag gtctacaaga
      181 tggccctgat caccgccgca ctgatctgag ttaaaaattc tacataaaat caccgccgcc
      241 cattgtgcaa tacagtgggt ggcggttttt aggttgtaag ggtcgatgct tgtatcgtcc
      301 cgcgggcgga tgtaaacagc cgcccctaca aaaagctgcc cgctgcgatt ttcagcagcg
      361 ggcagtttta tttattcatc aataatgcag cttatccgca tccagatttt ctagcactgg
      421 cagcatggcg gaggcttcgg cgcgggcagc gatggggtcc atcatcaggt agttgccgca
      481 ttccacggcg gatgcacccg ggatatcacc ctcaaacgct gccatccagg caaaagcact
      541 gcgggtcagg tccagcgcct cggcaggcgt caggccgatg gtcagcagat aaaagccggt
      601 caggcagccc atgggaccga catagacgat gcccttgctg tacttgctgt tgcgggcata
      661 ggtcgcaaac aggtgttcca gcgtgtgggc tgcggcgggg gaaaggtaac tgcccttgtt
      721 gggctgcttc atgcggatgt cccaggtcag gatctcgccg ttctcgccat cctggcggga
      781 aagatacatc ccgggcagca gcttggtgtg gtcaacgcag aaactcgcga tacgttccat
      841 cgttgcgcct cacttcgcca tagcctggac aacaccgcga gcgcagtcca tcgagatgac
      901 catgccgtcc ttcagggtgc gggtggcgtt ggtagcaccg acgatgactg ccttgcccag
      961 cgtcaggccg acaatcgcgg agtggctgtt ggtgccagcc tcctcggtga tgataccggt
     1021 agcctcgcgc atgaagggca gggtgtcgtt ggtggtgaag ggcacgacca gaatctgacc
     1081 gggcttgaac ttcttggcag cctcggcggc gctgcggcag acgcagactt cgccttttgc
     1141 gttgtactga ccaatgccaa caccagccag cagggaatca ccgaccatgt ggaccttgat
     1201 catgttggtg gtgccggaca caccgaccgg aacaccagcg gtcacgacga ccaggtcgcc
     1261 gtcctgtacc aggccagcct cctgcgcctt ggcaacggac atgctgatga gctcatcggt
     1321 agaggtggca tactccatca tcagcggggt cacaccgcgg tagacattca gctggcggcg
     1381 ggtcgtctcg tccagcacgc aggcaatgat ttgggtgtcg gggcggaagc gactcagcag
     1441 gcgggcggtc tcgccgctct tggaggccgt gatgatagcg gacgcaccga tatcggcagc
     1501 agtggtgcag gcggcgtggc ccacagcagc gctgacattc aggcgggtgg aatccgagcc
     1561 ggagtgatga accaggctgt caaagttgga gtcggcctcg gtggtctcgg caatcgtagc
     1621 catggccttt aaggcttcga ccgggtactt gcccgcagcg gtctcgccgg agagcatgac
     1681 cgcgccggtg ccgtcgtaaa tggcattggc aacgtcggtg atctcggcac gggtcgggcg
     1741 ggggttgacg atcatggaat ccagcatctg ggtagcggtg atgcagattt taccggcgct
     1801 catggcgcgg tcgatcaagt gcttctgaat ggcaggaatc tcagcaaagt cgatctccac
     1861 gcccatatcg ccgcgggcga ccatgatgcc gtcagccaca gtcagaatct cgtcaatgtt
     1921 gtcaatgcct tcctggttct cgatcttggc aatgatgcgg attttggagt tgtgctcgtc
     1981 cagaatgtgg cgaatctcca taatgtcctg ggcgttgcgg gcgaagctgg cggaaatcag
     2041 gtcaaagccc tgctcgatgc caaacaggat atcgctggta tcgcgctggc tcatatacgg
     2101 catagtcagg tgtacgccag ggacattgac acccttcttg gtcttgatgg taccgtcatt
     2161 acagacagtg cagttgatat cggtgtcgcc gacttcatcg atgcgcagtt cgatcaggcc
     2221 gtcatccagc atgatgcggc cgccggcctt gacatcgcgg ggcagatcct tgtaggtaac
     2281 ggagcagatt tcattcgtgc cctccacatt gcgggatgtc agggtgaatt tctggccggt
     2341 gacgagcttc tcgacgccgt tcttgaactc acccaggcga atctcggggc ccttggtgtc
     2401 cagcatacag gcgatgggca ggtccagttc cttggacagg gcacgcacct tgtcaaaacg
     2461 acccttgtgt tcctcgtagt caccgtgaga aaagttgaag cgggcaacgt tcatgccgtt
     2521 ttccatcagc tggcgcagaa tgcccggctt atcggtggac ggacccatag tacatacgat
     2581 tttggtttta cgcatagaaa tgcctccgtt cgtttttcta cctatttata tactcttagc
     2641 aaacctcagc ggtattatac tacaagtttg caggggtaaa caagtgggaa aaagctctcc
     2701 tgcacggttt tgccgcgcat tttttgcttt ttttcggtgc gttatttttt agacaaattc
     2761 agcctcgtcc gtacaaattc tgcggcagct ttggcgttgc ctggtgggta agaggtaggc
     2821 aggctgaccg atggccgtca gggcgttgcg ctccttgacg acggctttca gcgcggcata
     2881 gtcagcggcg tgttcggctt gctcggtcag ctcacgcagt ttttgctggg cggccgcata
     2941 atccggccgt gtgtagggga gtgtgctgaa tttttgtacc tgcatggcag ttccttttta
     3001 taaggaatat aaaaatccta ctcttaccta ttttacagta tagcccaagt cacgccgatt
     3061 tgcaacctcg gatttcatgt ttactttttg tccgttttac ggtataataa gcctaagaat
     3121 atcacagacc catttttata ttaggagaat catcatggca aaacgtgtat ttctgatcgt
     3181 actggacagc ttcggtgttg gcgcagagcc ggatgcaccg cttttcggcg accccggcac
     3241 caatacgctg ggggccattg caggccaccc caacttcaag ggtgataacc ttgccaagct
     3301 cggtatgttc aacctggacg gtgtcacctg cggcacgccg gtgtctgcgc ccatcggcag
     3361 ctatgcccgc ctgcgcgagg ccagtgccgg caaggacacc accatcggcc attgggagat
     3421 tgcgggcctg ctcagcgcag agccactgcc caccttcccg aacggcttcc cgcaggagct
     3481 gctggacgag ttcaccgcca agaccggcta caaggttctg tgcaacaagc cctactccgg
     3541 caccgaggtc atccgcgact acggcgagga gcacatgaaa accggtgccc tcatcgtcta
     3601 cacctccgcc gacagtgtgt tccagattgc cgccaacgag gcccttgtgc ccatcgaaaa
     3661 gctctacgag atctgcaccc aggcgcgtga gctgttgacc ggcaagtacg gtgttggccg
     3721 tgtgattgcc cggcccttcg ttggcgactg tgccgccaac tttacccgca cgccccgccg
     3781 ccacgattac agcctggtgc cgccgcatga cacgatgctg gacgccatcc aggccgcagg
     3841 caaggcaact atcggcgtgg gcaaaattca tgatatcttt gcgggcaagg gcatcggcga
     3901 gaccatccgc accagcggca acaccgaagg cttgcaggtc acgctggatc tggctgaccg
     3961 cgattttgag ggtctggctt ttgtcaacct cgttgacttt gatatgctct acggccaccg
     4021 ccgtgatatc gccggttatg ccgccgctgc cgccgagttc aacgactggc tgcccggctt
     4081 tatggccaag atgtgcccgg gcgatatcct gatggtcacg gctgaccacg gctgcgaccc
     4141 cagctacacc aagaccaccg accacacccg cgagtatgtg ccgttcctga tttacggcga
     4201 cgaggtcaag cccggcgtca acctgcacac ccggtacagc tttgccacga tcgccgacac
     4261 cgtctgcaag gcactgaacg ttgactactg cccggcaggc tgcggcgtct acgatgagat
     4321 caaaaagtaa cgtggggctg tttctgctgt atctgctggc ggcggcgctc gttgtttact
     4381 cggtctcgct ggtgattttg tccaacttca acatgggcaa tctcatggtc tggctgctga
     4441 ccgcctgcgt tgtcggatat gcggtgttcc gccgtccgct ccacgcgtgg ttcagccacg
     4501 gcacaggccg ggtcgtattc tgggtactgg cggcactggc tgccatctac ttggccgtca
     4561 ttgcgtttgt gtctgtcagc ggctacacga acccgcctac cgggaatgag caggtcgtcc
     4621 ttgtgctggg cgcgggcctg cacaaggaca agcccagtaa gctgctgcaa taccgcctga
     4681 ataaagccta tgagttcgct gccgcccacc cggacacact ggtcatcacc agcggcgggc
     4741 aggggcgcga cgagtggtgc cccgagggtg acgccatgcg ggactacctt attgccaagg
     4801 gactggagcc tgcgcgggtc atatcagagg ggcgttcgac ctccacggaa gaaaactttg
     4861 ccttctcgct cgccattctg cgccagcacg gctatgacga gacaacgccc atcgtctatg
     4921 tttccaatgc gttccactgc taccgcgccg gaaaatatgc tgccatggcg ggctttacca
     4981 atgccaccga gctggctgcc ggcacgccgc tgcgcagcgt attaccgtgc tacctgcggg
     5041 aggcgctggc acttttgtat tactgggtat tcaagacttc cgtcaccggg ccgatgcatg
     5101 ctatggtcgg tttgctggat atgaataaga aatttttcta taaataggag ggaactgcca
     5161 tgcagatccg ctattataaa gagtacagcc gcttcctgaa ccgctttatg gagttcaagg
     5221 tctacggcca cgccggacgc cccatcgtga tttttccgtg ccagagcggc cggttcttcg
     5281 attgggagga tcgcaatatg tgcaacgccg ccgcgccgtg gatcgacaac ggtaagctgc
     5341 agatcttcgc ggtggacagt atcgaccctg aaagctggga taaccacggc ccagagcgcc
     5401 cgcgtatcga aatgcaggag cgctggtata actatatctg cgaggaattt atcccccgac
     5461 tgctggagat caaccgtgag cagggagagg atcacaccgg tcgcatcctg actggcggtg
     5521 ccagcatggg cggcggccac gcggtcaact tctacctgcg tcgccccgat atcttcaacg
     5581 gtacgattgc cctgagcggc ctgtattcct ccggaatgtt ctttggcaat tacatggacg
     5641 atctggtcta ccgtaattct ccctgcgatt atatgcgcaa ctttcccacg actcatccgt
     5701 acatgaaact gtttgaaaag gccgacaagt tcattatgtg ctgcggccag ggcgcgtggg
     5761 aggctgacct gctggcctcc accagggagt tgcaggctat cctcgagagc aagggcatcc
     5821 accccatcgt tgatatctgg ggccaggatg tctcccacga ctggtactgg tgggagaagc
     5881 agtgggtcta cttcctgcag atgacgctgg gcgacgcctg atacaaaagg aaagtgctta
     5941 tggatttttg gcacgatgca gcggcgcaaa agcgctggct gcgccgtttt gtgctgctga
     6001 cagcggtgct gcttgtgccg gtgtttgcac tggcggtatt tgcgcgcccc tcggccgatg
     6061 actacatcta cgcggcccgc acccatgcgg tggtgcagca gtacggcttt gatctgccgc
     6121 gtctgctgcg tgccgcctgg gatacaaacg cctactatta cgaaaactgg caagggctct
     6181 acgtttccgg ctttacgctg gccttccagc ccgctatctt cggcaacaaa tggtatggca
     6241 tcacgctttt gtgtgtgctt ctgccgctgt ttttctgcct gtacggcctt atgaggtgcg
     6301 ttgtgctgcg gctggacccg gcgcagaagc atctaccctg ggcgctggcg ctgctgctga
     6361 cctttgcgtt tatccagggg atgccctccc cggtggaagg gctgtactgg ttcaacggcg
     6421 caatgaacta cctgccgtat ttctcgctgg ccgcgctgaa tgccgggctt gcgtttgcac
     6481 tctgctttgc cgggaaattg gtgccccgcc aaaaagtgct ctacgcgctg acgggttgcg
     6541 tgtgcagcct tgtcatcggc ggcggccatc aggtagccgg gcttttgaac gtgctggtgt
     6601 tgctgttggc cgcggtgctc tgcgcacggc gccgcaattt ctggcaactg cccgcatttt
     6661 tcgctgctgt ccttggcctt ttgctgaacg tgctggctcc gggtacgcgg gtgcgcaccg
     6721 ccggctttgc gggagcgggc ttcgcggagg caatagtaaa aagttttatt ttggccgcta
     6781 tgcaatggct gcgctggctg gatgtgccgc tgctctgcct attggcattg ttggcgctgc
     6841 cgcttttgca gctggcccgc agcgcggttc tgcccgatcg ggtattccgc cgtccctggc
     6901 tgggcgcagc agtcactttt gtgctgatgt gggctatgat tttcctgccc tcctacacga
     6961 tgggcggcat cggcgcgggt cggcttctga acgtcgtctg gatgactttt gtgctgggcc
     7021 ttgccgccac cgaatttctg ctgctgggct ggctggagcg cgtgcgcggc gtatcgctgc
     7081 accgcgccga gcggttctgc cagcaccatg cccgccgctt gccgctggcc gttgcctgta
     7141 tgctggtggt gatggcctgt atcggcagcc acacggtcaa agagggccaa gacaactact
     7201 ttgccaccag cctggaagcc gcctatgagc tggccgacgg ccaggcccag cgctatgccg
     7261 ccgcgctgga tgaacgagag gcaatcctga ccgacgaagc ccagccggaa gtatccatcc
     7321 gcccactgaa cgagaacgag cgcccctggc tgctgtttta tactgacgtt gcccccgggc
     7381 cggacctgtg gggcctgaca ccctatttta ccaagcaatc agtgacaatt ttggattttg
     7441 aataaccatg cccctgccta gccgcagggg ttttgcatgg gcgtatttcc tattggcaaa
     7501 gtaggaaact ataccatacg attctggcac acattatcat tatatagaag gggcatcccc
     7561 atgatctatc ttgactattc tgccaacacc ccggcagacc ccgccgtgct ggacgcctat
     7621 gtggcggccg agcgacggta tattgccaat cccaattcca cccacattgc cggtcaggag
     7681 gcccgtgccg agatggagcg ggtgacgacg tcgattgcaa accacctcgg cgtgcagcct
     7741 gcggaaatca tctacacctc cggcgccagc gaagccaaca acctggccat caagggcatt
     7801 gcccaggcgt cgcgtcacat cggcaagcac atcatttcta cccagcttga gcattcttcc
     7861 atcggtgcgt cactctcggt cctgcagcag caagggtatg agattgacct gctcaacatc
     7921 aaccgcgatg gcagggtaga tttggatcag ctgcgggaac tgatgcgcaa tgatacgatt
     7981 ctggttgccg tctgcgccgt ggacagcgag ctt
//
LOCUS       DBMDSQ010000052         8011 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_2433_length_8011_cov_1.572946, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000052 DBMDSQ010000000
VERSION     DBMDSQ010000052.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 8011)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8011)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8011
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2433_length_8011_cov_1.572946"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..321)
                     /locus_tag="ACZPAX_03175"
     CDS             complement(<1..321)
                     /locus_tag="ACZPAX_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HIR1112932.1"
                     /translation="MKYSFPAFENGFIRAAAASPALRVADCAYNADQIIGVMREYAEK
                     NVQLLCLPEFALTGYTCSDLFLQETLLRGAEDGLAAILKASEGLNLVVLVGLPVRHNG
                     KLYNC"
     gene            491..688
                     /locus_tag="ACZPAX_03180"
     CDS             491..688
                     /locus_tag="ACZPAX_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002606951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR1112933.1"
                     /translation="MAKNLKLKAARAVRDMTQADLAAAVGVSRQTINAIEKGEYNPTI
                     NLCRSICRVLGKTLDELFWEE"
     gene            692..1168
                     /locus_tag="ACZPAX_03185"
     CDS             692..1168
                     /locus_tag="ACZPAX_03185"
                     /inference="COORDINATES: protein motif:HMM:NF042802.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6773 family protein"
                     /protein_id="HIR1112934.1"
                     /translation="MNLREIFKRENLDEMQKQTLLKIESRGFWGLWVLLLAALIIESL
                     LGFAPREMAAEWFIFMLGSAYSVISDLRAGIWDRHLKANTKTNAVVSVAGGAAVFVWG
                     LIKFAALGTGIAVLQAVIMGVCTWVLCFALLQLSMKAYKKRHAELENPKEDDDENE"
     gene            1155..2144
                     /locus_tag="ACZPAX_03190"
     CDS             1155..2144
                     /locus_tag="ACZPAX_03190"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HIR1112935.1"
                     /translation="MKTNKKELTVFALCAYAIPFLMMPLLYICLQNGQDTSIFANAQM
                     FYPAAGVMLAFLLARRPDTPKRFYSLHIVSAVLMLVMSVLSVAMPQTGWLTIANVAII
                     IASVLGWILLLTEKKERREAYGLRLHGKFLTALAICVYFLAVKTGMVFLSVAMQGGDT
                     WANYLAYWRSPTPWIMAMALIPNFFLSFLPFFGEEYGWRCYLTPALQNRFGARRGALA
                     VGVLWGLWHLPLNLFFYSPETTLQSIASQLVVCMTLGVFFTFAYEKCGKNIWVSILLH
                     YLNNNMILVWTGTADISNQVIHWADVGVSAVMYAAVFLPFLAAKCYRRGEKAV"
     gene            complement(2263..2718)
                     /locus_tag="ACZPAX_03195"
     CDS             complement(2263..2718)
                     /locus_tag="ACZPAX_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HIR1112936.1"
                     /translation="MYGVSKINSEQNIMLTTFPGAQYSAQSLAEHLDVFAKAGIVVDM
                     ICQSAPRGAAVDFSFTTSYDNFAAVMKALPAAAKANPPLVSGGYSKINLFGEEMVTSC
                     GVAARALAALSGANVEIVLITTSDLDISLLIRQQDEDAALDALHKAFAV"
     gene            complement(2746..3975)
                     /locus_tag="ACZPAX_03200"
     CDS             complement(2746..3975)
                     /locus_tag="ACZPAX_03200"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HIR1112937.1"
                     /translation="MRMKGNDLAACLVIALGVGGTIVGSLLAPVNEPVTLPAVNPQPV
                     MMETPAPEPTTAPEPTPEPTPTVETVHFSATGDDLIHDGIFLQAKERGGDHYDFDAAY
                     AAMQGYYDQFDVNWLNQETLVNDEFAASGYPMFSTPGAITDTLYNLGFRVFSLSNNHS
                     YDKGAAGIEASMAHWAAMPDDVVTMGFYNLSTYDDYVYQTVNGITFGYLSYTEHTNGL
                     PTPSGAEYGVVYLDDRETIAKQIADMRPNCDVLIVSAHMGTEGTHEINDFQKQTAQWL
                     ADQGVDVIIGTHPHVVQNAEWLTGANGNTTFVVYSLGNFINAQSSADNTIGAILDVYF
                     DKTTQPDGSSSITIRDPKLHCVISQYEAGYKNIRVIPYSQYTDELGAAHGVFTLSRAY
                     IESVLYGAIDEQFITLD"
     gene            complement(3966..4733)
                     /gene="dapB"
                     /locus_tag="ACZPAX_03205"
     CDS             complement(3966..4733)
                     /gene="dapB"
                     /locus_tag="ACZPAX_03205"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HIR1112938.1"
                     /translation="MTDIIIQGIYGRMGRALLEKIGARDDCRVVAGVDREAGNVGGIP
                     VYAGFAELPCKGVIIDFSSPAGAVAAAQYGAKNGLPCVICSTGLSKEDEAVLEAASAK
                     VPIFRSANMSVGVNVLIELARQATKILGGEFDIEIVEKHHHNKLDAPSGTALMIADAI
                     NAEAGGKYEYVYDRSQVRQKRGAQELGISSIRGGGIVGEHDVLFCGPDEVVTLSHSAG
                     SRGVFADGAVQAALYIAALAPGYYTMTDLLRGKLVCE"
     gene            complement(4800..5690)
                     /gene="dapA"
                     /locus_tag="ACZPAX_03210"
     CDS             complement(4800..5690)
                     /gene="dapA"
                     /locus_tag="ACZPAX_03210"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HIR1112939.1"
                     /translation="MKKPVFTGAAVAVITPMNADGSVNYDELGCIIEDQIAHSTDAIV
                     ICGTTGESPTLTDEEHTECIRYTVKKVAGRVPVIAGTGSNDTKYAIWLSQQAEADGAD
                     ALLLVTPYYNKTSQAGLIAHYTAIADAVHLPCILYNVPSRTGCSLTPATLAELAKHPN
                     INAVKEASGNISQVAEIAAACGDNLNIYSGNDDQIVPLLALGGKGVISVLSNVAPQYT
                     HDICAKWFAGDTAGSLSMQLKALPLIKALFADVNPIPVKWAMNRLGWQAGECRLPLVA
                     SSAAVQAQLENAMQDFGLLK"
     gene            complement(5731..6807)
                     /gene="asd"
                     /locus_tag="ACZPAX_03215"
     CDS             complement(5731..6807)
                     /gene="asd"
                     /locus_tag="ACZPAX_03215"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019679217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HIR1112940.1"
                     /translation="MKQYNVGVIGATGMVGQRFITLLENHPWFNLVALAASARSAGKT
                     YEEAVGSRWLMQTPMPESVKKMVVLDAANVEEVASKVDFVFCAVNMKKDEIKALEEAY
                     AKAECPVVSNNSAHRFTPDVPMVVPEINADHIEIIPAQRKRLGTKRGFVAVKSNCSLQ
                     SYVPALHPLRKYGITDVLVCTYQAISGAGKTFDTFPDILDNVIPYIGGEEEKSEQEPL
                     KLWGRIENDKIVPATAPRFTAQCLRVPVSDGHMGAVFVRFENKPSKEEILQAWKEFHG
                     PAQDLNLPSAPKQFLHYFEENDRPQPKLDRMLENGMAVSIGRLREDTQYDYKFVCLSH
                     NTLRGAAGGGVLLAELLAAKGYFD"
     regulatory      complement(6934..7110)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            7180..>8011
                     /gene="queA"
                     /locus_tag="ACZPAX_03220"
     CDS             7180..>8011
                     /gene="queA"
                     /locus_tag="ACZPAX_03220"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005942082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HIR1112941.1"
                     /translation="MLKKDFWYDLPKELIAQEPANPRDAARLMVLDRKNDKILHSVFH
                     ELPHYLEKGDLLVVNNSKVLPARLMGTKVPTGAVCELLLLRQVKGDTWECLARPGKRM
                     QPGTKVEFGDGSLTAVVDETMPDGNKYVTFTYDTETLYEKLDEFGKMPLPPYITKQLE
                     DQSQYQTVYAKELGSAAAPTAGLHFTPQLMDTIRSRGVNIAEVTLHVGLGTFRPVNEE
                     SIEDHQMHSEWYSVSEETAKLINDTRAAGHRVIAVGTTSCRTLESVWDRYGKIVPCSG
                     N"
BASE COUNT         1654 a         2237 c         2407 g         1713 t
ORIGIN      
        1 gcagttgtag agcttgccgt tgtggcgtac cggcagcccg acgaggacaa cgaggttcag
       61 cccttcgctg gctttcaaaa tcgcggcaag gccgtcctcg gccccgcgca ggagcgtttc
      121 ctgcaagaac aggtcgctgc aggtgtagcc ggtcagcgca aactccggca ggcagagcag
      181 ctggacgttt ttctcggcgt attcgcgcat gacgccgatg atctggtcgg cgttgtaggc
      241 gcagtcggcc acgcgcaggg cggggctggc tgcggcggcg cggatgaatc cattttcaaa
      301 agcgggaaag ctgtacttca tagccaaact tcctttatca taatataaaa ctatcatagc
      361 acagcaggcc gcttgccgca agtatgtgtt ttgtgtgcaa aatataaatt tcaaaaagta
      421 agatatactt gacaaaatgt aatgcaaatg atatagtaaa gccacagcaa gaggaaagga
      481 acgtaaggct atggcaaaaa acctgaagct gaaggccgcc cgcgccgtgc gcgatatgac
      541 gcaggcggat ctggcggcgg cggtgggcgt gtcccgccag accatcaacg ccattgaaaa
      601 aggggagtat aaccccacga tcaacctgtg ccgcagcatc tgcagggtgc tgggcaagac
      661 attggacgag ctgttctggg aggaataagc aatgaatctg agggaaattt ttaagcgcga
      721 gaatctggac gaaatgcaaa agcagaccct tttgaagatc gagtcccgcg gcttctgggg
      781 gctgtgggtg ctgctgctgg cggcgcttat catcgaaagt ctgctgggct ttgccccgcg
      841 ggagatggcg gcagaatggt ttatctttat gctggggagt gcctattcgg tcataagtga
      901 tctgcgtgcc ggtatctggg accgccacct taaggcgaac acgaaaacca atgccgttgt
      961 gtccgttgcg ggcggtgctg ccgtgtttgt gtgggggctt atcaaatttg cagcgcttgg
     1021 cacgggcatt gcggtgctgc aggcggtcat catgggggtc tgtacctggg tgctgtgctt
     1081 tgcattgctg cagctcagca tgaaggctta caaaaagcgc cacgctgaac tggaaaaccc
     1141 caaggaggac gacgatgaaa acgaataaaa aagagctgac tgtctttgcg ctctgtgctt
     1201 acgcaattcc gtttctgatg atgccgcttt tgtacatctg cctgcagaac gggcaggata
     1261 caagcatttt tgccaacgcc cagatgttct acccggcggc gggtgtcatg ctggccttcc
     1321 tgctggcgcg gcgaccggat acgcccaagc ggttttacag tctgcacatc gtgtccgccg
     1381 tgctgatgct ggttatgagt gtgttgtccg tggctatgcc gcagacgggg tggctgacca
     1441 ttgccaacgt tgccattatt attgcctcgg tgctgggctg gatactgctg ctgaccgaga
     1501 aaaaggagcg ccgtgaagcc tacggcttgc gcctgcacgg caaatttttg acggcacttg
     1561 ctatctgcgt gtacttcctc gcagtcaaga ccggcatggt gttcctctcg gtggcaatgc
     1621 agggcgggga tacctgggcc aactacctgg cctactggcg cagccccaca ccgtggatca
     1681 tggcgatggc gctgattccc aatttctttc tgtccttcct gccgttcttt ggcgaggaat
     1741 acggttggcg ctgctacctg acgcccgccc tgcaaaaccg cttcggtgcg cggcgcggtg
     1801 cgctggccgt gggtgtgctg tggggcttgt ggcatctgcc gctgaacctg ttcttttaca
     1861 gcccggaaac aaccttgcag agtatcgcgt cgcagcttgt tgtctgtatg acgcttggcg
     1921 tatttttcac attcgcctac gaaaaatgcg gcaaaaacat ttgggtgtcc atactgctgc
     1981 attatttgaa caataatatg attctcgtct ggacaggcac ggcggatatc tccaaccagg
     2041 tcatccactg ggcggacgtg ggtgtcagcg ccgtgatgta tgcggccgtg ttcctgccgt
     2101 ttttggcggc aaagtgctac cgccgtgggg aaaaggcggt gtaattctac ggaaattcga
     2161 taacagaagt gcatagtaag ctttgcatca ccaaaacaaa ataaaccccg ccgggcattc
     2221 ccaaaagaaa agcccggcgg ggttgctgct tctgctatta aattacaccg caaacgcctt
     2281 gtgcagtgcg tcgagggcgg cgtcctcgtc ctgttggcgg atgagcaggg aaatgtccag
     2341 gtcgctggtc gtgatgagca cgatctcgac attggcaccg gacagggcag ccagggcgcg
     2401 ggcggccacg ccgcagcttg tgaccatttc ctcaccgaag aggttgatct tgctgtaccc
     2461 gccggaaaca agcgggggat tggccttggc cgcagcgggc agggccttca tgacagcggc
     2521 aaagttgtca tagctcgtgg tgaagctgaa gtccaccgct gcgccgcgcg gggcgctctg
     2581 gcagatcata tccaccacaa tgcctgcctt ggcgaacaca tccagatgct cggccaggct
     2641 ttgggcgctg tactgcgcac cgggaaaggt ggtgagcatg atgttctgct cactgttgat
     2701 tttgctgaca ccgtacataa acaatcatcc tttctatgca atatgttaat ccaacgtaat
     2761 aaactgctca tcaatcgcac cgtaaagcac actttcaatg tatgcgcggg agagggtaaa
     2821 cacgccgtgg gctgcgccca gttcgtcggt atactggctg tacgggatga cgcggatatt
     2881 cttgtagccg gcctcatact ggctgatgac gcagtgcagc ttcgggtcgc gtatcgtgat
     2941 gctggaactg ccgtccggct gggtcgtttt gtcaaagtaa acgtccagaa tcgcaccgat
     3001 ggtattgtcg gccgaggact gggcgttgat aaagttgccc aggctgtaca ccacaaaggt
     3061 cgtgttgccg ttggccccgg tcagccattc ggcgttctgc accacatggg ggtgggtgcc
     3121 gatgataaca tcaacgccct gatcagccag ccattgggcg gtctgcttct ggaagtcgtt
     3181 gatctcgtgc gtgccttcgg tgcccatgtg ggcgctgacg atgagcacat cacagttggg
     3241 gcgcatatcg gcaatctgct tggcaatcgt ctcccggtcg tcgaggtaca caacgccgta
     3301 ctccgcgccc gagggcgtcg gcaggccgtt cgtgtgttcg gtgtaggata ggtagccgaa
     3361 agtgatgccg ttgacggtct ggtagacgta gtcgtcgtag gtgctgagat tgtaaaagcc
     3421 catcgtgacg acatcgtccg gcatggcggc ccagtgcgcc atggaagcct caatgcctgc
     3481 cgcacccttg tcgtagctgt ggttgttgga caggctgaat acccggaatc ccagattata
     3541 cagcgtgtcg gtgatggccc cgggcgttga gaacatcggg tagccgctgg cggcaaactc
     3601 atcgttgaca agtgtttcct gattgagcca gttcacgtca aactggtcgt agtagccctg
     3661 catcgcggca taggcggcgt caaaatcgta gtggtcgccg cctcgttctt tcgcctgcaa
     3721 gaaaataccg tcgtggatca ggtcgtcgcc ggtcgcggaa aaatgcacag tctccacggt
     3781 gggtgtcggc tcgggcgttg gctccggtgc agtggtgggt tccggggcag gcgtttccat
     3841 catgaccggc tgcggattga cggcaggcag ggtcacaggc tcattgactg gggccagcag
     3901 ggagccgact atcgtaccgc ccacgcccag tgcaattacc aggcaggcgg caaggtcgtt
     3961 gcctttcatt cgcatacgag tttaccccgc agcaggtcgg tcatcgtgta gtaaccgggg
     4021 gccagcgctg caatgtagag cgcggcctgc acggcaccat cggcaaaaac accgcggctg
     4081 cctgcactgt ggctcagggt cacgacctcg tcggggccgc agaacagcac atcgtgctcg
     4141 cccacaatgc cgccgccgcg gatggaactg atacccagtt cctgcgcacc gcgcttctgg
     4201 cgcacctggg agcggtcgta gacgtactca tacttgccgc ccgcctcggc gttgatagca
     4261 tcggcaatca tcagtgccgt gccgctgggg gcgtccagct tgttgtggtg gtgcttctcg
     4321 acaatttcaa tgtcgaactc tccgccaagg atcttcgtgg cctggcgggc cagttcgatc
     4381 agcacattca cgcccaccga catatttgca ctgcggaaga tgggcacctt ggcgctggcg
     4441 gcctccagca cggcctcatc ctccttggac aggccggtgg agcagatgac gcagggcagg
     4501 ccgttcttgg caccgtactg tgcggcggcc accgccccgg cgggggaaga aaagtcgatg
     4561 ataacgccct tgcagggcag ctcggcaaag cctgcgtaga ccgggatgcc gcccacattg
     4621 cccgcctcac ggtcaacacc ggccacgaca cggcagtcgt cacgcgcacc gattttttcc
     4681 agcagggcgc ggcccatgcg gccatagatg ccctgaatga tgatatccgt cattgttacg
     4741 tcctttcgtg aaaaaagcga aaagccgacc cgcaaaaatg cagtgggtcg gctgtgaaat
     4801 tatttcagca ggccgaaatc ctgcatggcg ttttccagct gtgcttgcac agcggcagag
     4861 gaggcgacca gcggcagacg gcattcaccg gcctgccagc ccaagcggtt catggcccac
     4921 ttgaccggga tggggtttac atcggcaaac agcgccttga tgagaggcag ggctttcagc
     4981 tgcatggaca ggctcccggc ggtgtcacct gcaaaccact tggcgcagat gtcgtgggtg
     5041 tactggggcg cgacgttgga cagcacgctg atgacgccct tgccgcccag tgccagcagc
     5101 ggcacaatct ggtcatcgtt gccggagtag atgttcaggt tatccccaca ggccgccgca
     5161 atctcagcca cctggctgat gttgccgctg gcctctttca cggcgttgat gttcgggtgt
     5221 ttggcaagct cggccagcgt ggcgggggtc aggctgcagc cggtgcggct gggcacattg
     5281 tacaggatgc agggcaggtg caccgcgtcc gcaatggccg tgtagtgggc gataaggcct
     5341 gcctggctgg tcttgttgta gtagggcgtg accagcagca gggcgtcggc accgtcggcc
     5401 tcagcctgct gggacagcca gatggcgtac ttggtatcgt tggagccggt acccgcaatc
     5461 acaggcacgc gcccggcaac ctttttgacc gtatagcgga tgcactcggt atgctcctcg
     5521 tcggtcagtg tcggggactc accggtcgtg ccgcagatga cgatggcgtc agtgctatgg
     5581 gcaatctggt cctcaatgat gcagcccagc tcgtcatagt tgacgctgcc gtctgcgttc
     5641 atcggggtga tgacggccac ggcggctcct gtaaaaacgg gtttcttcat acgaatttct
     5701 ccttacacca aagctgcttg gcgcagcaaa tcagtcaaaa tagcccttgg cggccagcag
     5761 ttcagccagc agcacaccgc cgccggcagc accgcgcagc gtgttatgtg acaggcagac
     5821 aaatttgtaa tcgtactggg tatcctcgcg caggcggccg atgctgacag ccatgccgtt
     5881 ttcaagcata cggtcgagct tgggctgcgg gcggtcgttt tcctcaaagt aatgcaggaa
     5941 ctgcttcggg gcgctgggca ggttcaaatc ctgtgccggg ccgtggaact ccttccaggc
     6001 ctgcaagatt tcttccttgc tgggcttatt ttcaaagcgc acgaacactg cacccatgtg
     6061 gccgtcagag accggcacac gcaggcactg ggccgtaaaa cggggcgcag tggcaggaac
     6121 gatcttatcg ttctcgatgc ggccccacag cttcagcggc tcctgttcgc tcttctcctc
     6181 ttcgccaccg atgtagggga tgacgttgtc gaggatgtca gggaaggtat caaacgtctt
     6241 gccagcgccg gaaatggcct ggtaggtgca gaccagcacg tcggtgatgc catacttgcg
     6301 cagcggatgc agggcaggca catagctctg caggctgcaa ttcgacttga cagccacaaa
     6361 gccgcgcttg gtgcccaggc gcttgcgctg ggcggggatg atctcgatgt ggtcagcatt
     6421 aatctcaggc acgaccatcg gcacatcagg ggtgaaacgg tgggcgctgt tgttggacac
     6481 gaccgggcac tcggccttgg cgtaggcttc ttccagcgcc ttgatctcgt ccttcttcat
     6541 gttgacggcg cagaacacga aatcgacctt ggaggcgact tcttccacgt tggcggcgtc
     6601 cagcacgacc atctttttaa cgctctcggg catgggagtc tgcatcagcc agcggctgcc
     6661 gacggcttcc tcataggttt ttcctgcgct gcgtgcactg gccgcgaggg caaccaaatt
     6721 aaaccagggg tgattttcca gcaacgtgat aaagcgctgg ccgaccatac cggttgcacc
     6781 gataacacca acattgtact gtttcatcct gactaagacc ttcctttcac gatacccttc
     6841 cttatatcta tgcgagttcc cgtaaaaatt tgcacagata ataaaaaaac cggcttgaaa
     6901 accggcacaa aatgcaatat aatatcatat tgcatataac acagcgcaac acactgattt
     6961 acagtgtgac agtcccgcac ctgtttggct gcggacccag gcggatgcgt gccgcgcatc
     7021 accttcggcg aaggaccctt tcccggtttc cgcccttggc aaggggataa ggaaaacggt
     7081 actgatgtgc ttcgcgcctc tgtgtttgga ttcattataa tattcctgca taggaatgtc
     7141 aagacaatag cgctttaaag tgaaaaagga tgagaaaata tgctgaaaaa agatttctgg
     7201 tatgacctgc cgaaagaact catcgcccag gaacccgcca atccccgcga tgctgcccgc
     7261 ctgatggtgc tggaccgcaa aaatgacaaa atcttgcact cagtctttca cgaactgccg
     7321 cactatctgg aaaagggcga cctgctggtc gtcaacaact ccaaggtgct gcctgcccgc
     7381 ctgatgggca ccaaggttcc cacgggtgcg gtttgtgagc tgctgctgct gcgccaggtc
     7441 aagggcgaca cctgggagtg cctggcccga ccgggtaagc gtatgcagcc cggcacgaaa
     7501 gtcgagttcg gcgacggcag cctgaccgct gtggtggacg agacgatgcc cgacggcaac
     7561 aaatacgtga cgttcaccta cgataccgag acgctgtacg agaaactgga cgagtttggc
     7621 aagatgccgc tgccgcctta catcacgaag caactcgagg atcagagcca gtaccagacc
     7681 gtctatgcga aggagctggg cagcgctgct gcgcctacgg cgggcctgca ctttaccccg
     7741 cagctgatgg acaccatccg ctcccgcggt gtcaacatcg ccgaggtcac gctgcacgtc
     7801 ggtcttggca ccttccgccc agtgaacgaa gaatccatcg aggaccacca aatgcacagc
     7861 gagtggtact ctgtcagcga ggagaccgca aagctcatca acgacacccg cgccgccggg
     7921 caccgtgtta ttgctgtggg taccacgagc tgccgcacgc tggaatccgt ctgggaccgc
     7981 tacggcaaga ttgtaccctg cagcggcaac a
//
LOCUS       DBMDSQ010000053         7960 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_2453_length_7960_cov_2.284642, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000053 DBMDSQ010000000
VERSION     DBMDSQ010000053.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 7960)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7960)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7960
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2453_length_7960_cov_2.284642"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="ACZPAX_03225"
     CDS             complement(<1..192)
                     /locus_tag="ACZPAX_03225"
                     /inference="COORDINATES: protein motif:HMM:NF014712.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyltransferase domain-containing protein"
                     /protein_id="HIR1112942.1"
                     /translation="MLSFQLHPVGDCALLAAFAQRIAPDIGAAVAALNSRVLAAKLPG
                     VVETVPAFASLLITYDPLVT"
     gene            complement(221..1282)
                     /locus_tag="ACZPAX_03230"
     CDS             complement(221..1282)
                     /locus_tag="ACZPAX_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003410407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4392 domain-containing protein"
                     /protein_id="HIR1112943.1"
                     /translation="MTRDELEKRNVGENLDALMNLDPRGYGVCRILYAGSRAFTGEPL
                     TMHAAQVLCDSIKENDLVYILVGFVLLPHKVPEMDGTVSAMLLARALVMAFNAKPVIV
                     CPADSVQAIEKCAAVVGLHIYEDLDTVQELPLSMGVVAFTKDKAAAPAQAEALAARKP
                     AAVISVEASGANALGVYHNAVGKDVTALQARSDVLWDILRANGVPNIAIGDLGNEIGM
                     GTIAEHIKKYVPFTAEGECQCGCGGGILSATKADNIITATCSDWGCYGLMAALAYLKK
                     DMEILHHEEMESEVMRVAARNGFIDMTGSLLPGIDGFSTRMNVGIVSLMRQCTAYAVR
                     YSHKSDHWFGPVLAKHFFD"
     gene            complement(1299..2156)
                     /locus_tag="ACZPAX_03235"
     CDS             complement(1299..2156)
                     /locus_tag="ACZPAX_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017752177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HIR1112944.1"
                     /translation="MEALAYYDGKIGTPEELTVPFNDRVHFFGDGVYDATVGANGKVY
                     LMQDHLDRFYTSAKALDINIPMPKEELGKLLTDLLAKVDGTTHFVYWQVTRGVETRNH
                     VYAENLPGKLWVSIRQNHLNDPDVPIKLNTEEDTRFYHCNIKTLNLLPSVVAAQHAKR
                     IGVQETVLHRGDIVTECAHSNVSILKDGVFYSHPNDEFILRGIAKTHMIQACYRLGIT
                     VMEKCFTLDDLMNADEIIVTSSSNFCLHACEVDGKLAGGKDPATLKAIQDEVLREYYA
                     YTGKTTVVD"
     gene            complement(2183..2833)
                     /locus_tag="ACZPAX_03240"
     CDS             complement(2183..2833)
                     /locus_tag="ACZPAX_03240"
                     /EC_number="2.7.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015394303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0050145 - nucleoside monophosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HIR1112945.1"
                     /translation="MYPNYPYFAVAITRTCGSGGGSYIGKKLAQDFDIDLYDRKLLRL
                     AAEDSGINEKIFAAADENTKKTLLYRVSQHVYDGSFIPPESGNFISDQNLFNYQAKIL
                     RELLDEESYVCVGRAADFVLKDKPNVLTVYIDAPYEDRIEREMKRQGIGRSQAMHYID
                     KLDHYRESYYKYHTGRQWKRVENYDLCLDSAAIGLDNCVEVIKRVIELKFGVKCPR"
     gene            complement(2844..3542)
                     /locus_tag="ACZPAX_03245"
     CDS             complement(2844..3542)
                     /locus_tag="ACZPAX_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013842288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1112946.1"
                     /translation="MLEIKDLHVNYGAVHALNGVSMTVKDGEIVSLIGANGAGKTTTL
                     RTITGLEKAAGGSITFDGHDLRKTEPSKIITLKLAHVPEGRHIFPQMTVEENLEMGAF
                     TDPTDMAKTMADVYERFPRLKERKRQLAGTLSGGEQQMLAVGRALMGKPKMILMDEPS
                     MGLSPLLVKEIFAIIREVNKQGITILLVEQNAKMALAISDRAYVLETGTITIEGDAKE
                     LLNDARVKKAYLGQ"
     gene            complement(3535..4326)
                     /locus_tag="ACZPAX_03250"
     CDS             complement(3535..4326)
                     /locus_tag="ACZPAX_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006874236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1112947.1"
                     /translation="MAAPVLKAQHLGIQFGGLKAVDDFNMEIGESELVGLIGPNGAGK
                     TTIFNLITGVYKPTEGSFYLCGERMNGKKTHQIVHAGIARTFQNIRLFKKMSVIENVK
                     TAMQAHTTYGMADAIFRTKKYWQQEAEITARAKELLEVVHLSGKEDLEADNLPYGEQR
                     RLEIARALATDMKLLLLDEPAAGMNPTETEELLEIINYIRNEFKVSVLLIEHDMSLVM
                     KICERIQVLDFGTTIASGTPEQIANDPHVIEAYLGKDEAKEAADA"
     gene            complement(4332..5327)
                     /locus_tag="ACZPAX_03255"
     CDS             complement(4332..5327)
                     /locus_tag="ACZPAX_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020074152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR1112948.1"
                     /translation="MTKRRLASYGLNLVGVVLLYAAITLTFTFNAFGKSTTYIQGICT
                     TACYTIIMVTSLNLVVGFMGEFSMGHAGFVSVGAYVSAIVSGALAGHGLSDLGLLLVA
                     ILAGGLAAGLMGVAVGIPALRLRGDYLAIVTMAFAEIIRVCFCNFSITGGGKTMSGIL
                     KLSTFDRAFWIMVVCVTIMFLFVRSRFGRTVQAIREDYIAASASGINVTYYKVLTFAV
                     SAFFAGIGGGVYAHYMTAMIPTNFNFNYSAELLSEVIIGGTGSLTGSIIGAAFLSSLP
                     ELMRDFSTYRMLAYSVVLVLVMLFRPGGIFGRWEFSLTRLLGRLAHPKVKAKKGA"
     gene            complement(5343..6233)
                     /locus_tag="ACZPAX_03260"
     CDS             complement(5343..6233)
                     /locus_tag="ACZPAX_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002579382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR1112949.1"
                     /translation="MSFSLFFQSLINGLNQGAIYALIALGYTMVYGIIRMINFAHGDF
                     IMIGAYTLFYTIPLMVNAGMPAWLAVIVAIAVCAVVGVLVETFAYRPVRKAGPMSALI
                     TALAMSIFLENLAMVLFGAKSHNVQAIFSLPTVTVGGVALPLNVLLTIAIGLGMMIAL
                     QLFVKKTKLGKAMRAVPQDKDASILVGINVNKIITTTFAIGSGLAAVAALMYCATYPR
                     VTNDMGSMMGMKAFIAAVLGGIGIIPGAMLGGILIGLIEIFVKLFAPGWYEAVTYGTL
                     IVILLVKPSGILGKNVGEKV"
     gene            complement(6351..7586)
                     /locus_tag="ACZPAX_03265"
     CDS             complement(6351..7586)
                     /locus_tag="ACZPAX_03265"
                     /inference="COORDINATES: protein motif:HMM:NF024850.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR1112950.1"
                     /translation="MKKRNRVLALTVAAAMSASLLAGCGSSAQQGGAASSETTTSTAA
                     AASDGGEDNIIRVGVVCSMTGGSAIYGEGAQNAIDMAVEEINAGDSGYKIEIVNGGKV
                     ADDAKDAKQAMNAYNKVMADSPEAIVGSFFSSVTLPMAEQASKDGMLLLATGATNADV
                     TLKGSTIFRNCFIDPYQGKMAALFAKDKGFTKAAVIYAKDDDYSNGLKDAFIENCEAN
                     GIEVAYTGECMTTDTDYSSQAAQAVASGAELLYYPCFLDTVPLLVGAARNAGFTGAIM
                     GGDGWDGSDTTGLSAQFDDCYFTNHYSSEDTAPAVQNFVQKYTEKYGTESLNACAALY
                     YDAMYMLVQAAENAGGTDTASLVKGMTGMSFTGVGGDITLDENGDAIKSIAINTYVDG
                     AVKWTETLGSDGKVVSKAE"
BASE COUNT         1631 a         2323 c         2246 g         1760 t
ORIGIN      
        1 cgttacaagt gggtcatacg tgatgagcag cgaggcaaag gcgggtactg tttccaccac
       61 gccggggagc tttgcggcca gcacacggct gttcagtgcc gccaccgccg cgccgatgtc
      121 tggcgcaatg cgctgtgcaa acgctgccag cagggcgcag tcccccaccg gatgcagctg
      181 aaacgacagc atggccgtcg cctccttcca tattatatag tcagtcaaag aaatgctttg
      241 ccagcaccgg gccgaaccag tgatccgatt tgtgggagta ccgcaccgcg taggccgtac
      301 actggcgcat caggctgacg atgcccacat tcatccgggt gctgaagcca tcgatgcctg
      361 gcagcagtga gccggtcata tcgataaacc cattgcgtgc cgccacccgc atgacctcgg
      421 attccatctc ctcatggtgg agaatctcca tatctttctt gagatatgcc agcgccgcca
      481 tcaggccgta acagccccag tcggagcagg tcgctgtgat gatgttgtcg gccttggtgg
      541 cgctcaaaat gccaccgccg cagccgcatt ggcactcgcc ctccgctgtg aatggcacgt
      601 actttttgat atgctcggca atcgtgccca tgccgatttc gttgcccaga tcgccgatgg
      661 cgatgttcgg cacgccgttg gcacgcagga tatcccacaa aacatcgctg cgggcctgca
      721 gggcggtcac atccttgccg acggcgttgt ggtaaacgcc cagcgcgttc gcgccgctgg
      781 cctccactga gatgacggcg gcaggcttgc gggcggccag cgcttcggcc tgcgccgggg
      841 cggcggcctt gtccttcgta aaggcgacga cgcccatgct taagggcagc tcctgcacgg
      901 tgtccagatc ctcgtagata tgcagcccca cgacagcagc gcatttctcg atggcctgca
      961 cactgtcagc cgggcagacg atgaccggct tggcattgaa cgccatcacc agcgcccgcg
     1021 ccaacagcat agcgctgacc gtgccgtcca tctccggcac cttgtggggc agcagcacaa
     1081 agccgaccaa aatgtacaca agatcgttct ctttgatcga atcacacagc acctgcgcgg
     1141 cgtgcatcgt cagcggctcg ccggtaaagg cgcggctgcc cgcgtataaa atgcggcaga
     1201 cgccgtagcc gcgcgggtca agattcatca gcgcatcaag gttttcgccc acgttgcgtt
     1261 tttccagttc atcgcgggtc atcttgcaca ccccccgttc agtccacgac ggtcgttttg
     1321 ccggtgtagg cgtagtattc gcgcagcacc tcatcctgga tggccttgag agtggccggg
     1381 tctttgccgc ccgcgagctt tccatccacc tcacaggcat gcaggcagaa gttggaggac
     1441 gatgtcacga tgatctcgtc ggcgttcatc agatcgtcca gcgtgaagca cttttccatg
     1501 accgtgatgc caaggcggta gcaggcctgg atcatgtggg tcttggcgat gccgcgcagg
     1561 ataaattcat cgttggggtg cgaataaaat acgccatcct tgaggatgga cacgttggag
     1621 tgggcacact cagtcacgat atcgccgcgg tggagcacag tctcctgcac accgatacgc
     1681 ttggcgtgct gcgccgccac aacgctaggc agcaggttca atgttttgat gttacagtga
     1741 tagaaacgcg tatcttcctc tgtgttgagt ttgatgggca catcggggtc gttcaggtgg
     1801 ttctggcgga tggataccca cagcttgccg gggagatttt cggcgtagac atggttgcgg
     1861 gtctcgacac cgcgcgtaac ctgccagtag acaaagtgcg ttgtaccgtc cactttggca
     1921 agcaagtcgg tcagcagctt gcccagttcc tctttcggca tcgggatgtt gatatccaat
     1981 gccttggcgc tggtgtaaaa gcggtcgagg tggtcctgca tcagatacac cttgccgttg
     2041 gcaccgactg tcgcgtcata cacgccgtcg ccgaagaagt gtacccggtc attgaaagga
     2101 accgtcaatt cttccggcgt gccgatttta ccgtcatagt atgcaagtgc ttccatcgtt
     2161 ctttcctccc tgattttgtt gttcatcgcg ggcactttac cccgaatttt aattcgatga
     2221 ccctcttgat gacctcgaca cagttgtcca ggccaatggc ggcgctgtcc aggcagaggt
     2281 catagttttc gacccgcttc cactggcggc cggtgtggta cttgtaatag ctctcccggt
     2341 agtgatccag cttatcaatg tagtgcatcg cctggctgcg gccgatgccc tggcgcttca
     2401 tctcccgctc gatgcgatcc tcataagggg catcgatgta gacagtcagc acattgggct
     2461 tatctttcag cacaaagtcc gccgcgcggc ctacgcagac atagctttcc tcgtccagca
     2521 gttctcgcag gatcttggcc tgatagttga agaggttctg gtcgctgatg aagttgccgc
     2581 tctccggcgg gatgaagctg ccgtcgtaga catgctgcga tacgcggtag agcagcgttt
     2641 tcttggtatt ctcatcggcg gcggcaaaaa ttttctcgtt gatgccgctg tcctcggctg
     2701 ccagacgcaa cagtttccgg tcgtacaggt cgatgtcaaa atcctgtgcc agctttttgc
     2761 cgatgtaact gccgccgccc gacccgcagg tgcgggtgat ggccacggcg aagtagggat
     2821 agttcggata catagtggcc cccttactgc cccagatagg ctttcttcac gcgggcgtcg
     2881 ttcagcaatt ccttggcgtc gccctcaatt gtaatcgtgc cggtttccag cacataggcg
     2941 cggtcggaga ttgccaacgc cattttcgca ttctgttcca ccagcaaaat cgtaatgccc
     3001 tgcttgttga cttcccggat aatggcaaaa atctccttga ccagcagcgg agacaggccc
     3061 atcgacggct catccatcaa aatcatcttg ggtttgccca tcagggcgcg gccaacggcc
     3121 agcatctgct gctcgccgcc ggagagcgta cccgccagct ggcgtttgcg ctcttttaag
     3181 cgggggaacc gctcataaac gtcggccatc gttttcgcca tgtcggttgg gtcggtgaac
     3241 gcccccatct caagattttc ttccaccgtc atctggggaa aaatgtgccg cccttccggc
     3301 acatgcgcca atttcaacgt aataattttg ctgggctcgg ttttgcgcag gtcgtgccca
     3361 tcaaaagtga tggagccgcc agccgccttt tccagcccgg taatcgtgcg cagggtcgtg
     3421 gttttgcccg cgccgtttgc gccaatcagg gaaacgatct cgccgtcctt caccgtcatg
     3481 gacaccccgt tcagcgcatg aactgcgccg tagttgacgt gcaggtcctt gatctcaagc
     3541 atcggcggcc tccttcgctt cgtccttgcc cagatacgcc tcgatgacgt gcgggtcgtt
     3601 ggcaatctgc tccggcgtac cggaggcgat cgtcgtgccg aaatccaaca cctggatgcg
     3661 ctcgcagatc ttcatgacaa ggctcatatc gtgctcgatg agcaggacgg atactttaaa
     3721 ttcattgcgg atatagttga taatttccag cagttcctcg gtctcggtgg ggttcatacc
     3781 cgccgccggt tcgtcaagga gcagcagttt catgtcggtg gccagtgcac gggcaatttc
     3841 caggcggcgc tgctcaccgt agggcaggtt gtcggcctcc aagtcctcct tgccggaaag
     3901 gtgaacaact tctagcagct ctttggcgcg ggcggtgatt tctgcctcct gctgccagta
     3961 tttcttggtg cggaagatgg catccgccat accgtaggta gtgtgggcct gcatagcggt
     4021 cttgacgttc tctatgaccg acattttttt gaacaggcgg atgttctgga acgtccgggc
     4081 aatgcccgcg tggacgattt gatgcgtctt ttttccgttc atccgctcac cgcagagata
     4141 gaacgagccc tcggtcggct tgtaaacgcc ggtaatcagg ttaaagatcg ttgtcttgcc
     4201 tgcgccgttg gggccgatga ggccgaccag ttcggactcg ccgatctcca tgttgaagtc
     4261 atccacggct ttcaggccgc cgaactggat acccagatgt tgggctttca atacgggtgc
     4321 cgccatatcg ctcacgctcc tttcttggct ttcaccttgg gatgggccaa gcggcccagc
     4381 aggcgggtca ggctgaactc ccaccggccg aagatgccgc ccgggcggaa cagcatgacc
     4441 agcaccagca cgacagagta ggccagcata cggtaggtgg agaagtcgcg catcagctcc
     4501 ggcagactgg acaggaacgc cgcgccgatg atggagccgg tcagcgagcc ggtgccgccg
     4561 atgatgacct cgctcagcag ctcggccgaa tagttgaagt tgaagttcgt ggggatcatc
     4621 gccgtcatat agtgggcgta gacgccgccg ccgatgcccg cgaagaacgc ggagaccgcg
     4681 aaggtcagca ccttgtaata ggtcacgttg atgccggagg ccgacgctgc gatgtaatcc
     4741 tcgcggatag cctgtaccgt acggccaaag cggctgcgca caaacaggaa catgatggtc
     4801 acgcagacga ccatgatcca gaacgcgcgg tcaaacgtcg aaagtttgag gataccgctc
     4861 atcgtcttgc cgccgccggt gatggaaaag ttgcagaaac acacgcggat gatttctgca
     4921 aaggccatgg tgacaatagc cagatagtcg ccccgcagcc gcagcgccgg gatgccgacc
     4981 gcaacgccca taagccctgc agccaggccg cccgccagaa tggcgacaag cagcagccct
     5041 aaatcagaaa gcccatgccc ggccagtgcg ccggagacga tggcggagac atatgcgccc
     5101 accgagacaa agcccgcgtg gcccatggaa aactcaccca taaagccaac gaccaggttc
     5161 agcgatgtga ccatgatgat ggtatagcag gcggtggtac aaatgccctg gatgtaggtc
     5221 gtgcttttgc caaaagcatt gaaggtaaag gtcagcgtga tggccgcata cagcagtacg
     5281 acaccgacca gattcagccc ataggaggcc agccgtcgtt tcgtcatctt gttcacgctc
     5341 ccttacactt tttcgccgac attcttgccc agaataccgg aaggcttgac cagcaggatc
     5401 acgatcaggg tgccgtaggt cacggcctcg taccagccgg gggcaaacag cttgacgaag
     5461 atttcgataa gaccaatcag aataccgccc agcatcgcac cggggatgat gccgatgccg
     5521 ccgagcacgg cagcgataaa cgccttcatg cccatcatcg agcccatgtc attcgtaaca
     5581 cgggggtagg ttgcacagta catcagcgcg gccacggcag ccagaccgga gccgatggca
     5641 aaggtcgtcg tgatgatttt gttgacattg atgcccacca aaatggacgc atccttgtcc
     5701 tgcggcacag cacgcatcgc cttgccgagt ttcgttttct tgacaaagag ctgcaaagca
     5761 atcatcatgc ccagcccgat agcaatggtc agcagcacat tcagcggcag cgccacaccg
     5821 cccacggtga cggtcggcag gctgaagata gcctgcacat tgtgcgactt ggcaccgaac
     5881 agcaccatgg ccaggttctc aaggaagatg ctcattgcca gcgccgtgat cagtgccgac
     5941 atcggcccgg ccttgcgcac agggcggtag gcgaacgttt ccaccagcac gcccacgacc
     6001 gcgcagacag cgatggcgac aatgaccgct agccaggccg gcatacctgc gttgaccatc
     6061 agcggaatcg tgtagaacag cgtgtatgcg ccaatcatga taaaatcacc gtgcgcgaag
     6121 ttgatcatac ggatgatgcc atagaccatt gtgtagccaa gggcaatcag tgcgtaaatc
     6181 gcgccctggt tcaatccatt gatcaggctt tggaaaaaca atgagaagct catttgtggt
     6241 ccattcccct ttcctacggt gtttcaaaaa aagaaatgta cacggcaggc gttccttgcg
     6301 ggttgcagca ccaccgtgta catctcccat ccgtctttgg actttaattt ttattcagcc
     6361 ttggaaacga ccttgccgtc agagccgagc gtttccgtcc acttgactgc gccgtccaca
     6421 taggtgttga tggcaatgct cttgatagcg tcgccgttct cgtccagcgt gatgtcgcca
     6481 ccgacaccgg tgaagctcat gccggtcatg cccttgacaa ggctggccgt atcagtgccg
     6541 cctgcattct cggcggcctg caccagcatg tacatagcgt cataatacag ggctgcgcag
     6601 gcgtttaggc tttcggtgcc gtacttttcc gtgtacttct gcacgaagtt ctggacagcc
     6661 ggggctgtat cttcgctgga gtagtggttc gtaaagtagc aatcatcaaa ctgtgcggaa
     6721 aggccggtcg tgtcggagcc atcccagccg tcaccaccca tgatagcacc agtaaagcct
     6781 gcattgcggg ctgcaccaac cagcagcgga acggtgtcga ggaagcaggg ataatacaaa
     6841 agctctgcac cgctggccac ggcctgcgct gcctgcgagg aataatcggt gtcagtggtc
     6901 atgcactcac cggtgtaggc aacctcgata ccgttggcct cgcagttttc aataaaggca
     6961 tccttcagac cgttggagta gtcatcgtcc ttggcgtaga tgaccgcggc cttggtaaag
     7021 cccttgtcct tggcgaacag cgctgccatc ttgccctgat acggatcaat gaagcagttg
     7081 cggaagatcg tagaaccctt cagggtgaca tcggcgttgg ttgcaccggt cgccagcagc
     7141 agcataccgt ccttggaggc ctgctctgcc atgggcagtg tgaccgaaga gaagaagctt
     7201 ccgacgatgg cctccggcga atccgccatg accttgttgt aggcgttcat ggcctgcttg
     7261 gcgtccttgg catcgtctgc gaccttgccg ccgttgacga tttcgatctt gtagccggag
     7321 tccccggcgt tgatttcctc gacagccata tcaatggcat tctgagcgcc ctcaccgtag
     7381 atggcggagc cgccggtcat cgagcagaca acaccaacgc gaatgatatt atcctcgccg
     7441 ccgtcggatg ccgcagcagc agtgctggtg gttgtctcac tggatgcggc cccgccctgc
     7501 tgggcactgg acccgcagcc tgccagtagg ctcgcggaca tagcggctgc aacggtaagt
     7561 gcaagaactc gattacgctt tttcatggtg tgccccctct ttctggatgt ttgccatttt
     7621 acaatagtcc ctctttcacc caaaacaaaa ggcgcacggc accgcaaatc cggtgctgca
     7681 cgcctttgtg cgaatacgaa aagggccgcg gcttttgcaa gtccgcgacc cctttgtccc
     7741 gttcgtgttc tgtgatgatg ctgttattat agcagactgc tcaaaaaacg caagtctatt
     7801 ttggtagttc cttcaaaatc attgtgtata gggcttttgg gccgaagatt gcaaagtttt
     7861 ctctgtatct ggcagaagat tgctaattgt ggcgcgtaca tctgtgtttt atgcgtgggc
     7921 agttgttcgt tacttatagg atagggatcc atgtactgat
//
LOCUS       DBMDSQ010000054         7943 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_2464_length_7943_cov_2.608646, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000054 DBMDSQ010000000
VERSION     DBMDSQ010000054.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 7943)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7943)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7943
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2464_length_7943_cov_2.608646"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1235)
                     /locus_tag="ACZPAX_03270"
     CDS             complement(<1..>1235)
                     /locus_tag="ACZPAX_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015537095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HIR1112951.1"
                     /translation="TINAIDKAIEIGMGKRTNTILQSAFFKLADVMPIDDAVEYMKAA
                     AKKSYGKKGDAVVQMNWKAIDAGLDAVHKVEVPASWSNPAADPAPKALKGPEALVKQI
                     RDVMEPIARMDGDSLPVSAFEGNVNGEWEQGASAYEKRGTAVMVPEWNAEKCIQCNQC
                     AFVCSHATIRPFCLTADEAAAAPESTKLADTKPKASAYKFTMAVSPLDCMGCGECVTV
                     CPTAAIEMKPQESQADQQAAFDYCVENIRKKDNIPGVVSEVSVKGSQFNQPLLEFSGS
                     CAGCAETSYARLITQLFGEKMFISNATGCSSIWGGTASISPYTTNKASGHGPAWINSL
                     FEDNAEHGLGMQIGYETVRENLITKVEALKGKSADLDAAIEKFLETKNNTKANDAPAK
                     ALVAALEADGSAEAAEILK"
     assembly_gap    1236..1245
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1246..2854)
                     /locus_tag="ACZPAX_03275"
                     /pseudo
     CDS             complement(1246..2854)
                     /locus_tag="ACZPAX_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005929090.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
     gene            complement(3154..3831)
                     /gene="tsaA"
                     /locus_tag="ACZPAX_03280"
     CDS             complement(3154..3831)
                     /gene="tsaA"
                     /locus_tag="ACZPAX_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014117962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HIR1112952.1"
                     /translation="MDYTIHPIATIHTDFADKFGIPRQSGLVPELTARIVFEPEYRDP
                     NALVGIEGYDRLWLIWQFSTVAAEYAAGKSWRPTVRPPRLGGNERRGVFATRSPYRPN
                     ALGLSCVELAGVKNGELIVRGADLLDGTPIFDIKPYLPYVDAHPDARGGFTDTTKEYA
                     LQVECPDTLLCKVPEEKRTALLGVLKNDPRPAYQHDPERVYALDFGGNNVKFTVAGDV
                     LTVTEIS"
     gene            4040..>4764
                     /locus_tag="ACZPAX_03285"
     CDS             4040..>4764
                     /locus_tag="ACZPAX_03285"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007048295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1112953.1"
                     /translation="MRLAPIIHTEKLRKVYAVGKERVVALNNVDLSIEKGEFCCIVGQ
                     SGSGKSTLLNQLAGLEKPTSGKVYIGKHEISKMTENELAAFRQQHLGFIFQSYNLLPT
                     MTAAENVALPLMFKGVGKKTRLTKARRELKAMGLLGRANHLPTEMSGGQQQRVGIARA
                     FVSSPKVIFADEPTGNLDSRTSKQVLYRMLEMSKTSHVTFVMVTHEPELAECADRIVT
                     ILDGKICSNVVQDEETKKKNRDAL"
     assembly_gap    4765..4774
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4942..6399
                     /locus_tag="ACZPAX_03290"
     CDS             4942..6399
                     /locus_tag="ACZPAX_03290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="COG1361 family protein"
                     /protein_id="HIR1112954.1"
                     /translation="MKRLFSLLTAFVLAAALCAPVLADDTTNATPDPTPTPTATIDAT
                     NSDGVYVSAYTVTDTAGNEISTIEVGDRINIVLKVVDHASARYCVKPEEISTRINSAV
                     FTYTGIGEIGQLFDNNDDPNATRLQNVRNGSASPEEITANAQYHYYSYVILFRDVIYN
                     GGGNTLPINLSYLDTSKPLQQFSVTLGQCVDKDQTTSPNLLVRTSSYGDQVTAGTEFP
                     LSLGIYATDGSEDLSDVIVSLTLPENISLSSGSLSLYVGDISPKQMRDITFQVMPSAG
                     FTGTVADITVNMSGTGVITGKAVTGTTTISVPISQPNRFEVGQLELSSDTIYVGDTGS
                     VTLSYVNKGKNPVSNLEARLTGTNLGAGSYQYLGNLNAGTEGSVDFDIAPDAAGIVSG
                     VITLNYEDASGNPQTISKDFSVSAEEMSMDDAYDPTMDGDVQPEQTGMPVWAWVLIGV
                     CGAVVVVVIIVVVVRKRKKAKALAALEAEDSDEDL"
     gene            6386..7786
                     /locus_tag="ACZPAX_03295"
     CDS             6386..7786
                     /locus_tag="ACZPAX_03295"
                     /inference="COORDINATES: protein motif:HMM:NF024116.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1112955.1"
                     /translation="MKISDEISLSARNLLRRKGRTALTLVGVVIGTCMVVLMISLGIA
                     QTKTNEEMLQSWGDLTQVQIYGYGMMMGSDGKPLYLDDAAIANIKQIPHVAAATPYAQ
                     ANNLEGEITAGRNGRYTSDIYNLIGIDPTALEPMGFALQSGSWPTNTPASEKATKLQV
                     LVGSSTGYNFQDSRKSYNSPKRYRYEGQTDANGKELPPFVDIDKDKMTLTIKTGEGST
                     EKSRSWELEIVGVLEPDGAKGYWTQSGIVLRIQDMKMLQKIYNDMTKTKEESKSYDQV
                     YVKVDDLSNVTDVETAIHDLGFTNTYSMNQQREEMQKQVMNSQMIFGGIAAVSLLVAA
                     INIINTMTMAIYERTREIGVMKVLGCELGNIRTMFLLESSTIGFIGGLIGLGISLIAS
                     FVLNNLSTLGQGLDLSGLMGGGYYMGGGGGTISIIPPWLMLAALVFATLVGLVAGILP
                     ANKAVKISALEAIRHE"
BASE COUNT         1658 a         2280 c         2409 g         1576 t
ORIGIN      
        1 tccttcagga tctcagcagc ctcggcactg ccgtcggcct cgagagcagc gaccagagcc
       61 ttggcgggag cgtcgttggc cttggtgttg ttcttggtct ccaggaactt ctcgatggca
      121 gcatccaggt cagcggactt gcccttcaga gcctcgacct tggtgatcag gttctcacgg
      181 acagtctcgt aaccgatctg catacccagg ccgtgctcgg cgttgtcctc gaacagggag
      241 ttgatccaag cgggaccatg gccggaagcc ttgttggtgg tgtacgggct gatagaagcg
      301 gtaccgcccc agatggagga gcagccggta gcgttggaga tgaacatctt ctcgccgaac
      361 agctgagtga tcaggcgggc gtaagaagtc tcggcgcagc ctgcgcagga gccagagaac
      421 tcaagcagcg gctggttgaa ctgagaaccc ttgacagaga cctcggaaac aacgccgggg
      481 atgttgtcct tcttgcggat gttctcgacg cagtagtcga aagcagcctg ctggtcggcc
      541 tggctctcct gcggcttcat ttcgatagca gcagtcgggc agacggtgac gcactcgccg
      601 cagcccatgc agtccagagg agacacagcc atggtgaact tgtacgcgct tgccttgggc
      661 ttggtgtcgg ccagcttggt gctctcggga gcagcggcgg cctcatcagc ggtcaggcag
      721 aacggacgga tggtggcatg agagcagacg aaggcacact ggttgcactg gatgcacttc
      781 tcagcgttcc actcgggaac catgacagcg gtgccgcgct tctcgtaagc ggaagcaccc
      841 tgctcccact cgccgttgac gttgccctcg aaggcggaaa caggcaggct gtcgccgtcc
      901 atacgagcga tgggctccat aacgtcacgg atctgcttga ccagagcctc gggtcccttc
      961 agggccttcg gagccgggtc agcagcgggg ttggaccagg aagcggggac ctcgacctta
     1021 tggacagcgt ccaggccggc gtcgatagcc ttccagttca tctggacaac agcgtcgccc
     1081 ttcttgccgt agctcttctt ggcggcggcc ttcatgtact cgacagcatc gtcgatgggc
     1141 atgacgtcgg ccagcttgaa gaaggcggac tgcaggatgg tgttggtgcg cttgcccatg
     1201 ccgatctcga tagccttatc gatagcgttg atggtnnnnn nnnnnccctg cttcgctgtt
     1261 gccgtctgct ttgaaggtgc tcatccagct cggcatcggt ccactggcag ttgatcatga
     1321 agacaccgcc cggcttgacg tcctggacca tcttcatgcc catgtggatg taagcggggt
     1381 tgtggcaggc aacgaagtca gcctggttga tgtagtaggg gctgcggatg ggcttgtcgc
     1441 cgaaacgcag gtggctgatg gtgacaccgc cggtcttctt ggagtcatac tggaagtaag
     1501 cctgaatgta cttgtcagta tggtcgccga tgatcttggt ggagttcttg ttggcgccga
     1561 cggtaccgtc accgcccagg ccccagaact tgcactcctt ggtgccgggg gcggaggtga
     1621 tgggagcggg cttgacctca ggcaggctga ggttggtcac gtcgtcggtg atgccgatgg
     1681 tgaagcgggg cttcggggca tccttctcca gctcggtgta gatagcaaag acgctggacg
     1741 gcggggtgtc cttggagccc aggccgtagc ggccgccggt caggacgacg tcgttcatgc
     1801 cggcctcacg cagggtggtg gcgacatcca ggtacagggg ctcgcccagg gagccgggct
     1861 ccttggtgcg gtccagaacg gcgatcttct tgacggtctt gggcaggacc ttcagcagag
     1921 cggaagagac ccagggacga tacaggcgga ccaggaccag gccgaccttc tcgcccttgg
     1981 cggtcaggta gtcaacaact tcctctgcaa cgtcgcagat ggagcccata gcgatgatga
     2041 cgcggtcagc gtcctcggcg ccgtagtagt tgaacaggtc gtagttggtg cccagcttct
     2101 cgttgatctt ggccatgtac ttctcaacaa cggcaggcag agcctcgtaa gcggggttgc
     2161 aggcctcacg atgctggaag aagacgtcgc cgttctcgtg agaaccgcgc atcttggggt
     2221 gctcggggtt cagggccttg gcacggaacg cctcgacagc ctccatgttg cacatttcct
     2281 tcaggtcctc gtagtcccac ttctcgatct tctggatctc gtgagaggta cggaaaccat
     2341 cgaagaagtt cacgaaggga acgtgaccct cgatagcgga caggtgagca acaggggaca
     2401 ggtccatgac ctcctggggg ttgctctcgg ccagcatggc aaagccggtc tgacgaacag
     2461 ccataacgtc gctgtggtca ccgaagatgt tcagggactg ggtagcaacg gtacgggcag
     2521 aaacgtcgaa gacgcagggc agctgctcag cggcgatctt gtacatgttg gggatcatca
     2581 gcaaaagacc ctgggaagcg gtaaaggtgg tggtgatagc accggtaccg agagaaccgt
     2641 ggacagcgcc tgcggcgccg gcctcggact ccatctcgac gaccttgacc tggttgccaa
     2701 agatgttctt caggcctgca gcactccact ggtcaacggt gtcggccatg gggctggagg
     2761 gggtgatggg gtagatggca gccacatcgg tgaacgcata agctacgtgc gccgcggcgg
     2821 tattgccgtc catagactgt ttagctctgg gcattttctt tcctccgttt ttattagagt
     2881 tttgcattat agagctgtgc ggttgtcagg gccgcacaaa tccagcgctg cctggcacgt
     2941 gccccaatgt tgctccgggc atagttccag ataaacgctt ttgcatagct ttattgtacc
     3001 aaaaaccccc tttttcgtaa aggggttttg ttcagaaagt tgcacagatt tttagagtcg
     3061 ttgtgtgcat tttgaccata gtgacgaacc aggcggattt tagggtgttt tgcagtacga
     3121 tttatgggat atcgttccgc atcgcggaat ttttcaggaa atttccgtca cggtcagcac
     3181 atcccccgcc acggtaaatt ttacgttatt gcccccaaaa tccagcgcgt acacccgctc
     3241 cgggtcgtgc tggtaggcag ggcgggggtc gttcttcagc acgcccagca gggcggtgcg
     3301 cttttcctcc ggcactttgc acaaaagggt atcggggcat tccacttgca gggcatactc
     3361 tttcgtcgtg tcggtaaagc cgccgcgggc gtcggggtgg gcgtccacgt agggcaggta
     3421 gggtttgatg tcgaaaatcg gcgtgccgtc caaaaggtcc gcgccgcgca cgatcagctc
     3481 gccgttcttg acacccgcca gctcaacgca ggacagaccc agggcgttgg ggcggtaggg
     3541 gctgcgtgtg gcgaacacgc cccgccgctc gttaccgccc aggcgcggcg ggcgcacggt
     3601 gggccgccag cttttgcccg cagcgtactc ggccgccacg gtggaaaact gccagatgag
     3661 ccaaagccgg tcgtagccct cgatgcccac aagggcgttg gggtcgcggt attccggctc
     3721 gaacacgatg cgggccgtca gttccggcac cagtccgctc tgccgtggta tgccgaattt
     3781 atcggcaaaa tccgtgtgga tggttgcgat gggatgaatt gtgtagtcca tggtaacatt
     3841 tgcccccttt ttgcgtttat tatataggaa agagagaaaa aagggaagta gggcggccag
     3901 tcctctggcc gccgcggggc attgcggcgg ccgcaatatg cccggcccct acatatgctt
     3961 cgcaaacttt ttacaactcc ccatcttcca cttttcgtcg aaatatggta caatctgttg
     4021 tatactgcta taaggaggtg tgcgcttggc acctatcatc catacagaga aactgcgcaa
     4081 ggtctatgcc gtcggcaaag agcgcgttgt agcgctgaat aacgtcgact tatcaataga
     4141 aaaaggcgag ttctgctgta tcgtcggcca atccggcagc ggcaaatcca cgctgctgaa
     4201 ccagctggcc gggctggaaa agccaacatc cggcaaggtc tacataggca aacacgagat
     4261 cagcaaaatg accgagaacg agctggccgc gttccgccag cagcatttgg ggttcatctt
     4321 ccagagctac aacctgctgc ccacgatgac ggcggcggag aatgtggccc tgccgctgat
     4381 gttcaagggc gtgggcaaga agacccgcct gaccaaggcc cgcagggagc tgaaagccat
     4441 ggggcttttg ggccgcgcca accatctgcc cactgaaatg agcggcggcc agcagcagcg
     4501 tgtcggcatt gcccgcgcgt tcgtcagcag ccccaaggtc atttttgccg acgaacccac
     4561 cggcaacctg gattcccgca cgagcaagca ggtgttgtac cggatgctgg aaatgtccaa
     4621 aaccagccat gtaacgtttg ttatggtcac gcatgagccg gaactggccg aatgtgccga
     4681 ccgcattgtg acgattttgg acggcaagat ctgttccaat gtcgtgcagg acgaggaaac
     4741 aaagaagaaa aaccgcgacg cgctnnnnnn nnnnaagcct tccccctagg gggaaggtgc
     4801 ccgaagggcg gatgaggggc gcgtgcccca cagccgaccc aaaagggggc aatgaccgca
     4861 aggccgcccc tcatcagccg cctgcgggcg gcagcttcct ccaaggggga agcctaagaa
     4921 aaaacggagg ttcaaactta catgaaacgc ctattctccc tgcttaccgc ttttgtgctg
     4981 gcggcggcct tgtgcgcgcc ggtgctggca gacgatacga cgaacgctac acctgacccc
     5041 acccccacgc cgacggcaac catcgatgcg accaactcgg acggtgtcta tgtctccgcc
     5101 tacaccgtca ccgatacggc aggcaatgaa atttcgacca ttgaggtcgg cgaccgcatc
     5161 aacatcgtgt taaaggtcgt agaccacgct tcggcccgct actgcgtcaa gccggaggaa
     5221 atctccaccc gcatcaactc ggcggtgttc acctacaccg gcatcggcga gatcggccag
     5281 ctgtttgaca acaacgacga ccccaacgcc acccgcctgc aaaatgtccg caacggctcg
     5341 gccagccccg aggaaatcac cgcgaacgcg cagtatcatt attacagcta cgtcattttg
     5401 ttccgcgacg tcatctacaa cggcggcgga aataccctgc ccatcaacct ttcctatctc
     5461 gatacctcga agccgctgca gcagttcagc gtgacgctgg gccagtgcgt ggacaaggac
     5521 cagaccacct cgcccaattt gctggtgcgc acgtccagct atggcgatca ggtcacggcg
     5581 ggcaccgagt tcccgctttc cctgggcatc tacgccaccg acggcagcga ggatctgagc
     5641 gacgttatcg tctcgctgac cctgcccgag aatatctcgc tgagcagcgg cagtctgtcc
     5701 ctgtatgtgg gcgatatctc ccccaagcag atgcgggata tcaccttcca ggtcatgccg
     5761 tcggcgggct ttaccggcac agtggccgac attaccgtca acatgagcgg caccggcgtt
     5821 atcaccggca aggccgtgac cggcacaacg accatctccg tgcctatcag ccagcccaac
     5881 cgctttgagg tcggccagct ggagctgtcc agcgacacga tctatgtcgg tgacaccggc
     5941 agcgtcaccc tttcttacgt caacaagggc aaaaaccctg tctccaactt ggaggcccgc
     6001 ctgaccggca ccaatctggg cgcgggcagc taccagtacc tcggcaacct gaacgccggc
     6061 accgagggca gcgtggattt tgacatcgcc cccgacgccg cgggcatcgt gtccggcgtt
     6121 atcacgctga actatgagga tgcctccggc aacccgcaga ccatctccaa ggacttttcc
     6181 gtcagcgccg aggagatgag catggacgac gcctacgacc ccacgatgga tggcgacgtc
     6241 cagcccgagc agaccggcat gccggtgtgg gcgtgggtgc tcatcggcgt atgcggcgcg
     6301 gtagtcgttg tggtaattat cgtggttgtg gtccgcaagc gcaagaaggc caaggcactg
     6361 gccgcactgg aagcagagga cagcgatgaa gatctctgat gaaatcagcc tgtcggcgcg
     6421 gaacctgctg cgccgcaagg gccgcacggc acttacgctg gtgggtgttg tcatcggcac
     6481 ctgcatggtc gtgctgatga tctcgctggg cattgcccag accaaaacga atgaggaaat
     6541 gctgcagagc tggggcgacc tgacccaggt gcagatctac ggctacggca tgatgatggg
     6601 cagcgacggc aaacctctgt atctggatga tgccgcgatc gccaacatca agcagattcc
     6661 ccatgtggcg gcggccacgc cctacgccca ggccaacaac ctggaaggtg aaattacagc
     6721 cgggcgcaat ggccgctaca cgtccgatat ttacaatctg atcggcattg accccacggc
     6781 cttggagccg atgggctttg ccctgcaaag cggcagctgg ccgaccaaca cccccgccag
     6841 cgagaaggcc accaagctgc aggtgctggt gggcagttcc accgggtaca actttcagga
     6901 ttcccgcaag agctacaaca gccccaagcg ctaccgttac gagggccaga ccgacgccaa
     6961 cggtaaagag ctgccgccct ttgtggacat cgacaaggac aagatgaccc tgaccatcaa
     7021 gactggcgaa ggctccaccg aaaagagccg cagctgggaa ttggagattg tgggcgttct
     7081 ggagccggac ggtgccaagg gctactggac ccagagcggt atcgtgctgc gcatccagga
     7141 catgaagatg ctgcaaaaga tctacaatga catgaccaag accaaggagg agagcaagag
     7201 ctacgaccag gtctatgtta aggtcgatga tctgagcaat gtcaccgacg ttgaaacagc
     7261 catccatgac ttggggttca cgaacaccta ctcgatgaac cagcagcgcg aggaaatgca
     7321 gaagcaggtc atgaacagcc agatgatttt cggcggcatt gcggcggtct cgctgctggt
     7381 ggcggctatc aacatcatca acactatgac gatggcgatt tacgagcgca ctcgtgagat
     7441 cggcgtcatg aaggttctgg gctgcgagct gggcaacatc cgcacgatgt tcttgctgga
     7501 aagctcaacc atcggcttca tcggcggcct gatcgggctg ggcatcagtc tgatcgccag
     7561 ctttgttttg aacaacctgt ccacgctggg gcaggggctg gacctgtccg gcctgatggg
     7621 cggcggctac tatatgggcg gcggcggtgg aacgatttcc attatcccgc cctggctgat
     7681 gctggcagcg ctggttttcg ccacgctggt gggcctggtg gccggcatcc tgcccgcaaa
     7741 caaggcggtc aagatcagcg ccctggaagc gatacgccac gaataaaaaa gcatcccgca
     7801 cagtttctgt gcgggatttt tgtatacaac ggttttttaa caacaaattg gacttttcca
     7861 ccgcaggttc ccacaaccaa aagaagtttt caacattttc cacaggaaaa gtagggtgca
     7921 tatgggtttt ccaccgagtt ttg
//
LOCUS       DBMDSQ010000055         7923 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_2472_length_7923_cov_1.504730, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000055 DBMDSQ010000000
VERSION     DBMDSQ010000055.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 7923)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7923)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7923
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2472_length_7923_cov_1.504730"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..496
                     /locus_tag="ACZPAX_03300"
     CDS             <1..496
                     /locus_tag="ACZPAX_03300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1112956.1"
                     /translation="LVVQYLALPDRALAITHSAMSAGGPLGGVLAGALAGRQHGRQKM
                     LTLWCISAVCALLGAAALPGVPSTVSYEAVTFLAFCQMAAAVPFQIDLVSSLQVQVPA
                     AQVGRCMSFVTFAICLTQPAGQTLYGLAYDRFAAQPSLVPLLAAAASIAINIAAARFF
                     CKAR"
     gene            797..1843
                     /gene="potA"
                     /locus_tag="ACZPAX_03305"
     CDS             797..1843
                     /gene="potA"
                     /locus_tag="ACZPAX_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:1902047 - polyamine transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein"
                     /protein_id="HIR1112957.1"
                     /translation="MNENLIVSLKDIVVDFDGQKVLDGLSLDIHDKEFVTFLGPSGCG
                     KTTTLRVIAGFVTPKSGNVFFDGKDIANMPPYKRPVNTVFQKYALFPHLNVFENVAFG
                     LRLKKMAEDEIRPKVLEMLETVGLKGFERRSIHQLSGGQQQRVAIARSLVNQPRVLLL
                     DEPLGALDLKLRKEMQLELKRLQREMNITFVYVTHDQEEALTMSDTVVVMSGGKIQQI
                     GSPQDIYNEPKNAFVARFIGDSNIVDGVMLRDFYVNFGGHDFTCVDRGFKPNEPVQVV
                     IRPEDVQIVPPSVGMLTGLVREVIFKGVHFEMHVDVEGYEWIIHSTQASQPGELIGMN
                     IGPDEIHIMKRSEV"
     gene            1847..2668
                     /locus_tag="ACZPAX_03310"
     CDS             1847..2668
                     /locus_tag="ACZPAX_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1112958.1"
                     /translation="MLKSKTSRWLAGPYLLWMAIFTVVPLVIVVWYAMTNTDGQFTLD
                     NLTQIGRYSSVFARSLILAAISTVVCLVMAFPVGYFLSRLRANKQHIMLMLIMLPMWM
                     NFLLRTYAWMTLLEKNGLINKFFGLFGLGPFNMINTSGAVVLGMVYNYLPFMILPIYT
                     AMTKIDDSIVEAAQDLGCNVWQILFRVLVPLTGPGIATGITQVFVPAVSTFIISRMLG
                     GGSNLLIGDLIELQFLGNSYNLNLGSAMSLVLMVIVLLCMSFTSSFDESEMEGVM"
     gene            2670..3521
                     /locus_tag="ACZPAX_03315"
     CDS             2670..3521
                     /locus_tag="ACZPAX_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1112959.1"
                     /translation="MAKKHSALLMRTYVILVFCFMYLPIAVMIAFSFNESKSRSNFTG
                     FTLGWYKSLFHNEMILSALGLSLVLALVSSVIATVLGTLATIGINSMSRRSQLIINNI
                     SYVPVVNPEIITGVSLMLLFVMAQSFGIGGESGIFGWPTLLIAHITFNIPYVIFNVGP
                     KLRQLDPSLYNAALDLGCTPRQAFSKVIMPQLSPAILSAFLICLTYSIDDFMISYFNS
                     GTMQTLPIAIYSMTRKKVSPEINALSAVMFCVILAIILISNAADSRAYRRNQNAIRKA
                     MQDEGGR"
     gene            3524..4765
                     /locus_tag="ACZPAX_03320"
     CDS             3524..4765
                     /locus_tag="ACZPAX_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR1112960.1"
                     /translation="MNWKKLSAAAMAALTVTIISAAPVLAADDIEVNEDISVSGDYDW
                     KRFANDHITLNVYNNGLYISDGSDDSVNVLSAFEDLTGIKVNYTTYDSNESLYAKLKS
                     GGVSYDVIFPSDYMVGKMANEGMLSELNMDNIPNFAGIGETYLDRSFDPGNKYSVPYM
                     WGTTGIIYNTTMVEEPPTKWADMWDVEYTNNVLMFNNSRDAYAIAAKTIGLSMNPQSV
                     DEITAITDALKKQKNIVQAYVMDEVFDKMEGGEAAMAPYYAGDAITMIDENPDLAFVH
                     PEEGVNFFIDNMCIPANAKHQEAAEMFINYLCEPDVGLANADFIGYSTPITAVWEMLD
                     DDLKYSEIAYPDAEVLDKAEVFETLPDDINAAMDAQWSEMKSFEEGGSGWMVVVLLML
                     ALLISAFNIWRKLRKKSRDNY"
     gene            4909..5652
                     /locus_tag="ACZPAX_03325"
     CDS             4909..5652
                     /locus_tag="ACZPAX_03325"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013782.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016297 - fatty acyl-[ACP] hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HIR1112961.1"
                     /translation="MPEKQTTYTEKFTVYRTDGDHLGNVKLGALLRYAQQVATAHAEA
                     VGLDDALYRKTHTAYVLAKLALHIERLPRVDEELTLLTQPENCKRAVNKRITHFYDAS
                     GAEAAVMDSRWVLIDTEKRIILRKHPEQFNDQWAADVPFELPIKLPRIAPEDCEVLGE
                     QQAVYSRCDMNGHLNNTRYADIVCDAVPWEVWDTAQIRDFTISYHREVPRGECFTLRR
                     ARPAEDTFYFEGEREGKSAFEAQLIFKHL"
     gene            5710..6570
                     /locus_tag="ACZPAX_03330"
     CDS             5710..6570
                     /locus_tag="ACZPAX_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005935120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraX family protein"
                     /protein_id="HIR1112962.1"
                     /translation="MTKQKFGLTGTALKTIALVLMVMDHIHYFFEFTGVVPEWFSMLA
                     RLSAPLFLFCTVEGFAHTHDRRRYFLRIWAIGTAMGTVEFFMIYAGAFRRGDGFYPMN
                     AIFQDLMLLCIIWQGIDWLRQRRFVRGALAAAMPILWPFCIVALLMLFPKIQDAPIGS
                     AVLAWVITAPLPLWTSITDGGWSFLAGGIVLYALYNHKRLRLSVWAAMTFLCDFVLVY
                     LQVHSLPDFAFSQMFTVYYEWLGVFAVIGMALYNGQRGSGHKQLFYWFYPAHVYLLYG
                     ASCLVYSLLQ"
     gene            6582..7253
                     /locus_tag="ACZPAX_03335"
     CDS             6582..7253
                     /locus_tag="ACZPAX_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005935122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR1112963.1"
                     /translation="MANILAVDDDRDLCTLLKTALERDGHTVETRTSGTQLTDTLCRW
                     ADCILLDVMMPGEDGFAVCRRIRAETDAPILFLTARTDEPSVLTGLGLGADDYLTKPF
                     RIAELRARVAAHLRRQNRTPSHKITRGGVALDLSAKEASVGGTPLHLTKSEYAICELL
                     ALHAGQTFSKEQIYEAVFGYDGTADDTAITQHIKNIRAKLRAAGAGEVLQTIWGVGYQ
                     WQNEN"
     gene            7235..>7923
                     /locus_tag="ACZPAX_03340"
     CDS             7235..>7923
                     /locus_tag="ACZPAX_03340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HIR1112964.1"
                     /translation="MAKRKLTSLRSVLLRYLLLCGGGCALILVLWWVIFMQLINIGFL
                     LPAVASAQACADARETVASMTADTFDSLQISDLCRWAVVQNDTVLQTNMTNRQLKIAL
                     NSYHGGSGNLVLMQYQYDVKMADGSFCLLQYDYALPYADPALRDTLPDFQTCYFVLLA
                     ALILVWLGWQTHCTVRVFAAETARLNAAVDAIAARQLEQIDTDGVRIREFDATLQALQ
                     TMGRELTDSLQ"
BASE COUNT         1618 a         2349 c         2172 g         1784 t
ORIGIN      
        1 cctcgttgtt caatatctgg cgctgcctga ccgagcactg gcgatcaccc actctgccat
       61 gagtgcgggc ggtcctttgg gcggtgttct ggcgggggcc ttggccgggc ggcagcacgg
      121 caggcagaaa atgctgacgc tctggtgcat cagcgccgtg tgcgctctgc tgggggccgc
      181 agcgctgccc ggcgtgccgt ccaccgtcag ctatgaggcg gtaacgtttc tggcattttg
      241 ccagatggcc gccgccgtgc cgtttcaaat cgaccttgtc agcagcttgc aagtccaggt
      301 gccagcggcg caggtgggcc gctgcatgag ctttgtcacc ttcgccatct gcctgaccca
      361 acccgccggg cagacactgt acggtcttgc ttatgaccgc tttgccgcgc agccctctct
      421 ggtgccgctg ctggccgccg cggcatccat tgcaataaac attgccgcag cgcgtttttt
      481 ctgcaaagca agataatttt acgaagtcat gaacacttct ttacaaaatc atgaaaaagt
      541 ttttattgac aaacattctc catgccgtat aatgtatctt gtgtgtgcat ctaagggtaa
      601 attttttctg ccctgacaca ttggcgttgt atgtgctgct tgcagcggac tttgcaaaac
      661 aaagaccgtg taacaaacct ttgcggggcg gcataattgg cgctgactcg tcaaggactt
      721 tgtgccgacc taaggaagaa acggcgcgtg aagaagccct gtaacgggaa attccgcaaa
      781 gggggatgca atgaaaatga atgaaaatct gatcgtatct ctgaaagaca ttgttgtcga
      841 tttcgatggg caaaaggttt tggacggcct ttcgctggac atccacgaca aggagtttgt
      901 cactttcctc ggcccttcgg gctgcggcaa gacgacgacc ctgcgtgtga ttgccggttt
      961 tgtcaccccg aaatcgggca atgtcttttt tgatggcaag gacatcgcca atatgccgcc
     1021 gtacaaacgg ccggtgaaca cggtgttcca gaagtatgcg ctgttcccgc acctgaacgt
     1081 gtttgagaat gttgccttcg gcctgcgcct gaaaaaaatg gcagaggatg aaatccgccc
     1141 gaaagtactt gaaatgcttg agaccgtcgg cctgaaaggc tttgagcgcc gcagcatcca
     1201 tcagctttcc ggcggccagc agcagcgcgt ggccattgcc cgcagcctgg tcaaccagcc
     1261 ccgcgtcctg ctgctcgacg aacctctcgg tgcccttgac ctcaagctgc gcaaagagat
     1321 gcagctggaa ctgaaacgcc tgcagcgcga aatgaacatt accttcgtct acgtcaccca
     1381 tgaccaggag gaagcattga ccatgtcgga caccgtcgtg gtcatgtccg gcggcaagat
     1441 tcagcagatc ggctcccccc aggatatcta taacgaaccc aagaacgctt ttgtggcccg
     1501 ctttatcggt gactccaaca tcgtggacgg cgtcatgctg cgcgacttct atgtcaactt
     1561 cggtgggcat gactttacct gcgtggaccg cggcttcaag cccaacgagc ctgtgcaggt
     1621 cgtcatccgc cccgaggacg tgcagatcgt gccgccttcc gtcggtatgc tgaccggcct
     1681 tgtgcgtgag gtcatcttca agggcgttca ctttgaaatg cacgtcgatg tggaagggta
     1741 tgagtggatc attcattcta cacaggccag ccagcccggc gagcttatcg gcatgaacat
     1801 cggcccggac gaaattcata tcatgaagcg ttcggaggtg taatagatgc tcaaatccaa
     1861 aacctcccgc tggctggcag ggccgtatct gctctggatg gccattttca ctgtcgtgcc
     1921 gctggtcatc gtcgtctggt acgccatgac gaacaccgac gggcagttca cccttgacaa
     1981 cctgacccag attggtcggt attccagcgt gtttgcccgc agcttgattc tggccgccat
     2041 ttccacggta gtctgcctgg tcatggcgtt cccggtcggg tattttctct cccgcctgcg
     2101 cgccaacaag cagcatatca tgctcatgct tattatgctg cccatgtgga tgaacttcct
     2161 gctgcgcacc tacgcatgga tgaccctgct tgagaaaaac ggccttatca acaaattctt
     2221 cggtctgttc ggccttggcc ccttcaacat gatcaatacc tccggcgcgg tcgtgctggg
     2281 catggtctac aactatctgc ccttcatgat tctgcccatc tacacggcca tgacgaaaat
     2341 cgatgattcc atcgtcgagg ccgcgcagga tctgggctgc aacgtctggc agattttgtt
     2401 ccgcgtgctc gtaccgctga ccggccccgg cattgccacc ggcattaccc aggtgttcgt
     2461 gcccgccgtc tccaccttta ttatcagccg tatgctgggc ggcggctcca acctgctgat
     2521 cggcgacttg atcgagctgc agttcctcgg caactcctat aacctgaacc tcggctctgc
     2581 catgagcctg gtgctgatgg tcattgtcct gctctgcatg agcttcactt cgagcttcga
     2641 cgagtccgag atggaaggag tgatgtgaca tggcgaaaaa acattccgcg ctgctcatgc
     2701 gcacctacgt cattctggtg ttttgcttca tgtacctgcc catcgcggtc atgattgcat
     2761 tcagctttaa cgagagcaaa tcccgctcca acttcaccgg cttcacgctg ggctggtaca
     2821 agagcctgtt ccacaacgag atgattttgt cggccctagg gctcagcctg gtgctggcac
     2881 tggtttccag cgtcatcgcc acggtgctgg gcacgctggc gaccatcggt atcaactcga
     2941 tgtcccgccg cagccagctc atcatcaaca acatcagcta tgtgccggtc gtcaacccgg
     3001 agatcatcac cggcgtttcg ctgatgctgc tcttcgtcat ggcgcagagc ttcggtatcg
     3061 gcggcgagag cggcatcttc ggctggccca cgctgctgat tgctcacatt acctttaaca
     3121 tcccttacgt tatctttaat gtaggcccca agctgcgcca gcttgacccg agtttgtaca
     3181 acgccgcgct ggaccttggc tgcacgccgc gccaggcatt ctccaaggtc atcatgccgc
     3241 agctgtcccc ggcaattctg tcggcgttcc tcatctgcct gacctactcc atcgacgatt
     3301 tcatgatttc ctacttcaac tccggcacga tgcagacgct gcccatcgcc atctactcga
     3361 tgacccgcaa aaaggtcagc cccgagatta acgccctgtc agcggtcatg ttctgcgtca
     3421 ttctggcaat catcctgatt tccaacgccg cggattcccg cgcctaccgc cgcaaccaga
     3481 acgccatccg taaggctatg caggacgaag gggggcgctg aagatgaact ggaaaaaact
     3541 ttccgccgcc gcaatggcag ccctgactgt cacgataata agtgctgccc cagtgctggc
     3601 cgccgatgac atcgaagtca acgaggatat ttccgtctcc ggcgactacg attggaagcg
     3661 ctttgccaac gaccatatca cgctgaatgt ctacaacaat ggcctgtaca tctccgacgg
     3721 ctctgacgac agcgtaaatg tgctgtccgc ctttgaggat ttgaccggca tcaaggtcaa
     3781 ctacacgacc tacgactcca acgagagcct ctatgccaag ctgaaatccg gcggtgtgtc
     3841 gtatgacgtc atcttcccgt cggactacat ggtcggtaag atggcaaacg agggcatgct
     3901 gtccgagctg aacatggaca acatcccgaa ctttgccggc atcggcgaga cctaccttga
     3961 ccgcagcttt gaccccggca acaagtacag tgtgccctac atgtggggca caacgggcat
     4021 catctacaac acgacgatgg tcgaggagcc gccgacgaaa tgggcggaca tgtgggacgt
     4081 cgagtacacg aacaatgtcc tgatgttcaa caactcccgc gacgcctacg ccatcgccgc
     4141 caaaacgatt ggcctgtcga tgaacccgca gtcggtggac gagatcaccg ccatcaccga
     4201 cgcgctgaaa aagcagaaga acatcgtgca ggcctacgtc atggacgagg tcttcgacaa
     4261 gatggagggc ggcgaggccg ccatggcgcc ctattacgcg ggcgacgcca tcacgatgat
     4321 cgacgagaac cccgatctgg ccttcgtcca cccggaggag ggcgtcaact tcttcatcga
     4381 caatatgtgc attccggcca acgccaagca tcaggaggcc gccgaaatgt tcatcaacta
     4441 cctgtgcgag ccggatgtcg gccttgccaa cgcagatttc atcggctatt ccacccccat
     4501 caccgccgtg tgggaaatgc tggacgatga tctgaagtac agcgagatcg cgtacccgga
     4561 tgcggaggta ctcgataagg cagaggtctt tgagacgctg cccgacgaca tcaacgccgc
     4621 catggatgcc cagtggagcg agatgaagag ctttgaggag ggcggcagcg gctggatggt
     4681 cgttgtgctg ctgatgctcg cgctcctcat ttccgcattc aacatctggc gcaagctccg
     4741 caagaaatcg cgcgacaatt actgaccctc tgcagtgcat cgcttcatga tgcactgcat
     4801 ttttattttc actgcgctgg cacgccgctt tgcatttttg ctctgactgt gctatactcc
     4861 atttgagcat ttgtaatttt tgatttgatt ggtaaggagg gcgcatctat gcccgaaaag
     4921 caaaccacct atacagagaa attcaccgtc taccgcacgg acggcgacca cctcggcaat
     4981 gtcaagctgg gagcactgct gcgctatgcc cagcaggtcg ctaccgccca cgccgaggct
     5041 gtggggctgg acgatgccct gtaccgcaag acccacaccg cctatgtgct ggccaaactg
     5101 gccctgcaca ttgagcgcct gccccgcgtt gatgaggagc tgaccctgct cacccagccg
     5161 gaaaactgca agcgggctgt caacaagcgc atcacccatt tttatgatgc ctccggcgcg
     5221 gaggctgccg tcatggacag ccgctgggtg ctcattgata cagaaaagcg catcatcctg
     5281 cgcaagcacc ccgaacagtt caatgaccag tgggccgccg atgtcccgtt tgagctgccg
     5341 atcaagctgc cccgcatcgc ccctgaggac tgcgaggtgc tgggggagca gcaggctgtc
     5401 tactcccgct gcgatatgaa cgggcatctg aacaacactc gatatgccga catcgtctgc
     5461 gatgccgtac catgggaggt gtgggatacc gcgcaaatcc gcgattttac aatcagctac
     5521 caccgtgagg tcccccgcgg ggagtgcttc accctgcgcc gtgccagacc ggcagaggac
     5581 accttctatt ttgaaggcga acgggaaggc aaatctgcct ttgaggcaca gctgattttc
     5641 aaacacttat aatttctaca caaacccctg ttacactgtt tacatcgaca atgtaaagga
     5701 gttattatta tgacaaagca aaaattcggc ctgaccggca cggcgctcaa aaccattgcg
     5761 ctggtgctca tggtgatgga tcacattcac tacttttttg aatttacggg cgttgtaccg
     5821 gaatggttca gtatgctggc gcggctgtcc gcgccgctgt ttctgttctg cactgttgag
     5881 ggcttcgccc atacccatga ccgcaggcgc tacttcctgc gtatctgggc catcggtact
     5941 gcgatgggca cggtggaatt tttcatgatc tatgcagggg ccttccgccg cggggacggc
     6001 ttctacccga tgaacgccat ttttcaggat ctgatgctgc tctgcatcat ctggcagggc
     6061 atcgactggc tgcgccagcg ccggtttgta cggggcgcac tggctgctgc catgccgatt
     6121 ctctggccgt tttgcatcgt agcgctgctg atgctttttc ccaaaattca ggacgcaccg
     6181 atcggctctg ccgtgttggc gtgggtcatc accgcgccgc tgcctttgtg gacatccatc
     6241 accgatggcg gctggtcttt tctggccggg ggcatcgtgc tgtatgcact gtacaatcat
     6301 aagcgtctgc ggctgtccgt ctgggcagcc atgaccttcc tgtgtgattt tgtgctggtc
     6361 tatctgcagg ttcacagctt gccggatttc gccttttccc agatgttcac agtctactat
     6421 gagtggctcg gcgtctttgc cgttatcggc atggcgctgt acaacggcca gcgcggcagc
     6481 ggccacaagc agctgttcta ctggttctac ccggcacatg tctacctgct ttacggtgca
     6541 tcctgtcttg tttacagctt actgcaataa aaggggctat tatggcaaac attctggcgg
     6601 tcgatgatga ccgtgacctt tgtacgctgc taaaaacagc gcttgagcgt gacggccaca
     6661 ccgtagaaac gcgcacctcc ggcacacagc tgaccgatac gctctgccgc tgggcagact
     6721 gcatcctgct ggatgtgatg atgcccggtg aggacggctt tgccgtctgc cgccgcattc
     6781 gcgccgagac ggacgcgccg atcttatttt tgaccgcgcg taccgatgag ccgtcggtgc
     6841 tgacaggcct tggccttggc gcggacgatt acctgaccaa gccgttccgc atagcggagc
     6901 tgcgtgcgcg ggtcgcagca cacctgcggc ggcagaaccg cacaccgtcc cataagatca
     6961 cccgcggcgg ggtggcgctg gatctttccg ccaaggaagc cagcgttggc ggcacgcctt
     7021 tgcacctgac aaaatccgag tacgctatct gtgagctgct ggccctgcat gcagggcaga
     7081 ccttttccaa agaacaaatt tatgaagcgg tgttcggtta cgacggtacc gccgatgaca
     7141 cggccatcac ccagcacatc aagaacatcc gcgccaagct gcgcgcggcc ggggcagggg
     7201 aggtgctgca aacgatatgg ggagtaggct accaatggca aaacgaaaac tgacatccct
     7261 gcgcagtgtt ctgctgcggt atctcctttt gtgcggcggc gggtgcgcgt tgattctggt
     7321 actctggtgg gtcattttta tgcagctgat aaacatcggt tttctgctgc ctgccgtggc
     7381 gtccgcccag gcctgcgccg atgcccggga aactgtggcc tcgatgacgg ctgacacatt
     7441 cgattccctg cagatcagcg acctatgccg ctgggccgtg gtgcagaacg acacggtctt
     7501 gcaaacgaac atgaccaacc ggcagctgaa aattgctctc aactcctacc atggcggcag
     7561 cggcaatctg gtgctgatgc agtaccagta cgatgtcaaa atggcggacg gaagcttttg
     7621 ccttttacag tacgactatg ccttgcccta cgctgacccg gccctgcggg acacgctgcc
     7681 ggatttccag acctgctatt ttgttctgct ggcggcgctc atcctcgttt ggctgggctg
     7741 gcagacccac tgcaccgtgc gggtctttgc cgcagagact gcccggctga acgcggccgt
     7801 agatgctatt gccgcccggc agctggagca aattgacacg gatggcgtgc gcatccggga
     7861 atttgacgcc accttacagg ccttacagac gatgggccgc gaactgacgg acagcctgca
     7921 aag
//
LOCUS       DBMDSQ010000056         7880 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_2485_length_7880_cov_2.068646, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000056 DBMDSQ010000000
VERSION     DBMDSQ010000056.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 7880)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7880)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7880
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2485_length_7880_cov_2.068646"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..427
                     /locus_tag="ACZPAX_03345"
     CDS             <1..427
                     /locus_tag="ACZPAX_03345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1112965.1"
                     /translation="TVNSQDSGLLIGISAKVEIIVSEKENVFTVPRDAVGTNDDGSTY
                     VLRKTGGEGVDMTFEQVAVTEGDTNDYYVEVTGDDLNEGDVIRATADLTQGVETSSSG
                     DGMQAMENGDLYVGDPSNMPEGTVIMGGGDAPAGGPGGQ"
     gene            517..1209
                     /locus_tag="ACZPAX_03350"
     CDS             517..1209
                     /locus_tag="ACZPAX_03350"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006874254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1112966.1"
                     /translation="MQGIRKSYYIGQPNELEILHGIDLTVYPGEFVAIVGESGSGKST
                     LMNIIGVLDKPTAGEYVLDGVNIHDADDNDLAAIRNRKIGFVFQTYNLIGRQSALKNV
                     ELPMLYAGVPAGERTRRAKEWLDRVGMAERMKHQPNELSGGQKQRVAIARAMVNEPAL
                     ILADEPTGALDSQTSRVVMNLFHELHEKYHKTIVLITHNPELAEECQRVLTLRDGLIV
                     GERKGTGERAAF"
     gene            1196..2506
                     /locus_tag="ACZPAX_03355"
     CDS             1196..2506
                     /locus_tag="ACZPAX_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007045582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1112967.1"
                     /translation="MQLFENISMALTSVKSNKMRSLLTMLGIIIGIAAVIAIETVGNS
                     MTGSVMDSMSGMGASNISVTVTQKSSSSTSGTSQGVRLRRFMDSQPSDSDLITQDMMD
                     DFMATFPTQVDHIELSQQVGDGTIAKYGEPTTTIKTSVSGANTATLENLGADSQILYG
                     RWLDDEKDAGRRVACVSEKFVEQAVGGTAQEAIGKAVTLTINKNLYTFYIQGVYKYTE
                     DNYSSMFGGSDDDSIQTNFYIPLDVAKSIVGAGAGYQSITVVANGGSVNVTTFVDTVG
                     DFFASYYTRNDSWTASASSLASLLDSMSEMMSTISLGISAIAALSLLVGGIGVMNIMM
                     VSVTERTREIGTRKALGAPASAIRMQFITESVILCLIGGAIGIVLGLGLGAALSKAVG
                     YTARPSLAAIVIAVGFSMAIGVFFGYYPANKAAKLDPIEALRYE"
     gene            2773..4137
                     /locus_tag="ACZPAX_03360"
     CDS             2773..4137
                     /locus_tag="ACZPAX_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004034109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1112968.1"
                     /translation="MRCFEKLKKERLFTNRQLLTLLWPLIIEQALEILVGMADTVMVS
                     SVGEAAISGVSLVDMINQLIITLFGALATGGAVVTSQHLGAKRREEAAHSAGQLVTLS
                     AILGVGVAAFCLITRTAQLRLFFGTITDDVMQACLTYFTITALSFPFLALYNAGAAIF
                     RSTGNSAVSMKVSVIVNAINFCGNALCVFVLKMGVAGVAVPTLISRAVGAVIILSLAA
                     RPDYVLQITPRSVTRLEGKTVKSILYIGVPSAFENSLFQLGRVLVVSMISLFGTVHIS
                     ANAVANNLDAIGCIVGNAMGLAMITVVGRCVGAQDFEQTKYYTKKLLLWDYIAQGAVN
                     VTVLLLLNEILSMYTLTPETRALAFTLVMIHNGFSILLWPASFVLPNALRAANDVRYT
                     MVVATISMLVWRMGLSWVLCVNMGMGAVGVWYAMLVDWVCRVICFVARFATGAWKKNA
                     VKKV"
     gene            4153..4908
                     /locus_tag="ACZPAX_03365"
     CDS             4153..4908
                     /locus_tag="ACZPAX_03365"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005944181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HIR1112969.1"
                     /translation="MKFISWNVNGLRACLKKGFEDVFNTLDADFFCIQETKMQPGQAD
                     FAPEGYTEYIYSADKKGYSGTAIWAREPALSVRYGLDEDIHNHEGRAITLEYPDFYLV
                     NLYVPNSQNELARIDYRMQWEDDLRCYLQKLDAEKPVILCGDLNVAHEDIDLKNPGPN
                     RGAAGFSDQERGKLGELLAAGFTDSFRHLHPDATGMYSWWSMRFRARERNAGWRIDYF
                     LVSDRIADKIQTAAILMDVMGSDHCPVELDINL"
     gene            5199..5732
                     /locus_tag="ACZPAX_03370"
     CDS             5199..5732
                     /locus_tag="ACZPAX_03370"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR1112970.1"
                     /translation="MWALTNEEFTGLMQQYQKLIYTVCLQFVHEPHTAEDLTQDTFLS
                     AFSAIDRCEPQYYKQWLVRVAANKCKDHLKSAWVRKVDAPGDDALPEPRGAPGSDADD
                     PAVNLADRAGAAELEQLVRNLREPYGKIAVMYFLEHKESSEIAKLVGRPPATINSQLW
                     RAKLLLRQQITERRQNE"
     gene            5729..6259
                     /locus_tag="ACZPAX_03375"
     CDS             5729..6259
                     /locus_tag="ACZPAX_03375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1112971.1"
                     /translation="MSLYFDNEKLFDAEGHLTDEGLYALKDGTLDDLGALEAAEHLSF
                     CDYCLLRYTAMIEAAPDCMKQPMRDLIPQVQNLMRLRSFRIMTNRYVSAAAAVVLGFV
                     MWGAVSTFGLPDVSALTAQQPTTPHERFGTKFSTAVNGFYKSLDDTFKNFTLTAESGL
                     EQMRTKPIGGSTAKGE"
     gene            6263..6796
                     /locus_tag="ACZPAX_03380"
     CDS             6263..6796
                     /locus_tag="ACZPAX_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005943979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1112972.1"
                     /translation="MKKNGFLTFIFACIPGAGQMYYGYMQRGLSIAMILILCVMAATV
                     VEPLLFLCLVIWMYSFFDTYDLIRHMAAGEPKEDSLLVLGNYEEIKKLVPQHNRLIGW
                     GLVGFGVWALYETFVSTWLYTLLCNLVGDGYAYDIITGIPNVVIAALLIFAGLKLLGL
                     HPETKHGDDELPPYPHE"
     gene            7008..>7880
                     /locus_tag="ACZPAX_03385"
     CDS             7008..>7880
                     /locus_tag="ACZPAX_03385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1112973.1"
                     /translation="MDEKTTPKKTRRVGTLAFALVLILAGVLLMLYQIVPAFNLLQIV
                     KFSPVILIVLGIEMLVYSAKPDVLVKFDWLAMLGTAFTLCVVGAAALLPLAVSEWGPA
                     RSSAISRIETEKVDALYSALTADPELKAKTGYCGVNVWFNHGAGGSYTLQSGDDCVLN
                     ATLTGPYADAESFAADCIGIMQLAADKDLGFTSYHFSSDEDTDDGISYYLDCVASYPA
                     GLTAAQVAQRVTESYHYDGSSFSSEADRDEYIKTRLRDDIAEEYANAHDDVYPDDDYV
                     DAEVERRFNEQFGIA"
BASE COUNT         1633 a         2342 c         2399 g         1506 t
ORIGIN      
        1 cacggtcaac agccaggaca gcggcctgct catcggcatt tccgccaaag tggagatcat
       61 cgtcagcgag aaggagaacg tctttaccgt cccgcgtgat gcggtgggaa ccaacgacga
      121 tggctcgacc tacgtgctgc gcaagaccgg cggcgagggc gtggacatga cctttgagca
      181 ggtcgccgtc accgagggcg acaccaacga ttactacgtt gaagtcaccg gcgacgacct
      241 gaacgagggc gacgtcatcc gcgccacagc cgacctgacc cagggcgtgg agactagctc
      301 gtccggcgac gggatgcagg cgatggaaaa cggcgatctt tacgtcggcg accccagcaa
      361 tatgccggag ggcaccgtga tcatgggcgg cggcgacgcc ccggcgggcg gcccgggagg
      421 tcagtgatgc cggaaaatac acaacagttc gcgcaaaata tcgcgcagac cctgattggt
      481 gacgaggccg cgcaccccgc acagccgctc atccagatgc agggcatccg caaaagctac
      541 tacatcggcc agccgaacga gctggaaatc ctgcacggca ttgatttgac cgtctacccc
      601 ggcgagttcg tcgccatcgt gggtgagtcc ggctcgggca aatctacgct gatgaacatt
      661 atcggcgtgc tggacaagcc cacagcgggg gagtacgtgt tggacggcgt gaacatccac
      721 gacgccgacg acaacgactt ggcggccatc cgcaaccgca agataggctt tgtgttccag
      781 acctacaacc tgatcggccg ccagagtgcg ctgaaaaacg tcgaactgcc gatgctgtac
      841 gcgggcgtcc cggcgggcga gcgtacccgc cgcgccaagg agtggctgga ccgcgttggc
      901 atggccgagc gcatgaagca ccagccgaac gagctttccg gcggccagaa gcagcgtgtg
      961 gcgattgccc gcgccatggt caacgagcct gcgctgattc tggctgacga gccgaccggc
     1021 gcactggaca gccagaccag ccgcgtcgtg atgaacctct ttcacgaact gcatgaaaaa
     1081 taccacaaga ccatcgtgct catcacgcac aaccctgaac tggccgagga atgccagcgg
     1141 gtgctgacgc tgcgcgacgg cctgatcgtg ggcgaacgaa aggggacagg cgaacgtgca
     1201 gctttttgag aatatcagca tggcgctgac cagcgtcaag tccaacaaga tgcgcagcct
     1261 gctcacgatg ctgggtatca tcatcggcat tgcagcggtc atcgccattg aaacggtcgg
     1321 caacagcatg accggctcgg tcatggactc gatgtccggc atgggtgcca gcaacatttc
     1381 ggtcacggta acgcagaaaa gcagttccag tacctcgggc acatcccagg gtgtccggct
     1441 gcgccggttc atggactcgc agccgtcgga ttccgacctc atcacccagg atatgatgga
     1501 tgactttatg gccaccttcc cgacgcaggt ggatcacatc gaactttccc agcaagtagg
     1561 cgatgggacg attgccaagt acggcgaacc gaccacgacc atcaagacca gcgtcagcgg
     1621 tgccaatact gccacactgg aaaatcttgg cgcagattcg cagattttgt atgggcgctg
     1681 gcttgacgat gaaaaggacg cgggccgcag ggtcgcttgt gtgtcagaaa aattcgtcga
     1741 acaggctgtc ggcggcaccg cgcaggaggc catcggcaag gcggtcacgc tgacgatcaa
     1801 caagaatttg tatacgttct atatacaggg cgtatataaa tacacagagg ataactactc
     1861 cagcatgttc ggcggcagcg acgatgacag catccagacg aacttttata tcccactgga
     1921 cgtggcaaaa tccatcgtcg gcgcgggcgc aggctaccag agcatcacgg tggtggcaaa
     1981 cggcggcagc gtgaacgtga ccacctttgt ggacacggtg ggcgatttct ttgccagcta
     2041 ctacacccgc aacgattcct ggaccgccag cgcgtcgagc ctggcgagcc tgctggattc
     2101 gatgtctgaa atgatgagca cgatttcact gggcatttcg gccattgcgg cgctgtcgct
     2161 gctggtcggc ggcatcggcg tcatgaatat catgatggtc tccgtcaccg agcgcacccg
     2221 cgagatcggc acccgcaagg cgctgggggc ccccgcgtcg gccatccgga tgcagttcat
     2281 caccgagagc gttattctct gcctgattgg cggtgccatc ggcattgtgc tgggcctggg
     2341 gctgggcgca gcgttgagca aggccgtggg ctatacggcg cgcccgagtc ttgcggccat
     2401 tgtgattgcc gtagggttca gtatggcgat tggcgtgttc ttcgggtatt accccgccaa
     2461 taaagcggcg aagctggacc cgatcgaggc gctgcggtat gaataaaacc ccatgccttc
     2521 ccccttgggg ctgcgcccgc aggcgcgtgc cggagcgcaa ccgccgaagg cggctcttag
     2581 cgcgtagact gaaaggtggc cccgcagggc cggatgaggg gcgcgtctat ggtagtgtcc
     2641 ccgtaaagag gcagtaaggc aaggccgccc ctcatcagtc cgcttcgcgg acagcttccc
     2701 ccaaagggga agccttttat ctccccgcgc attgcgcggg gacttttttt gtggtatact
     2761 caacctggga aagtgagatg ctttgaaaaa ttgaaaaaag aaaggttatt taccaatcgg
     2821 cagctgctta cgctgttgtg gccgctcatc atagaacagg cattggaaat cctcgtaggt
     2881 atggcagata ctgtcatggt atcctcggtg ggtgaggccg ccatctccgg cgtttcgctt
     2941 gtcgatatga tcaaccagct tatcattacg ttgttcggcg cactggctac cggcggcgcg
     3001 gtcgttacca gccagcacct gggtgccaaa cggcgtgagg aggccgccca cagcgccgga
     3061 cagctcgtga cgctcagtgc cattctgggc gtgggcgtgg cggcgttctg ccttatcacc
     3121 cgcacggcac agctgcggct cttcttcggc acgatcaccg acgatgtcat gcaggcctgc
     3181 ctgacctact ttaccatcac ggcgctgtcc tttccgtttc tggcattgta caacgcgggc
     3241 gcggccatct tccgctccac cggcaacagc gcagtatcga tgaaagtatc ggtcatcgtc
     3301 aatgccatca acttctgcgg caacgcgctc tgcgtgtttg tgctgaagat gggcgtcgcg
     3361 ggcgtcgcgg tgcctacgct gatttcccgc gcggtgggcg cggttatcat cctgtcgctg
     3421 gcagcgcggc ccgactatgt gctgcagatc acgccccgca gcgtcacccg gctggaaggc
     3481 aaaaccgtca agagcatcct gtacatcggt gtcccctcgg cgtttgaaaa cagtctgttc
     3541 cagctgggcc gcgtgctggt cgtctcgatg atttcgctgt tcggcacggt gcatatctcg
     3601 gccaacgcgg tggcgaacaa cctggacgcc atcggctgca ttgtgggcaa cgccatgggc
     3661 cttgccatga ttaccgtcgt gggccgctgc gtcggtgcac aggactttga acaaacgaaa
     3721 tattacacca aaaagctgct gctgtgggat tacatcgccc agggcgcggt gaatgttacg
     3781 gtgctgctgc tgctcaacga gattttgagt atgtacaccc tgacgccgga gacccgggcg
     3841 ctggcgttca cgctggtcat gatccacaac ggcttttcaa ttttgctgtg gccggccagc
     3901 tttgtgctgc ccaatgccct gcgtgccgcc aacgatgtgc ggtacacgat ggtggtcgcc
     3961 acgatttcca tgctggtatg gcgcatgggc ctgagctggg tgctctgcgt caatatgggc
     4021 atgggtgcgg tcggcgtctg gtacgccatg ctggttgact gggtctgccg tgtgatttgt
     4081 tttgtcgcca gatttgccac cggggcgtgg aagaagaatg cggtgaaaaa agtataaaaa
     4141 ggagccaccc acatgaaatt tatcagctgg aatgtcaacg gactgcgggc ctgcctgaaa
     4201 aaggggtttg aggatgtgtt caacaccctt gacgcggatt tcttctgcat ccaggaaacg
     4261 aaaatgcagc ccgggcaggc ggattttgcg cccgaaggtt acaccgagta tatatacagc
     4321 gccgataaaa aaggctactc cggcacggcc atctgggcca gggaaccggc gctgtcggta
     4381 cggtacggcc tggacgagga tatccacaac cacgagggcc gcgccatcac gctggaatac
     4441 ccggactttt acctcgtgaa cctctatgtg cccaactccc agaacgagtt ggcccgcatc
     4501 gactaccgta tgcagtggga ggatgacctg cgctgctacc tgcaaaagct ggatgccgag
     4561 aagccggtca tcctgtgcgg ggacctgaac gtggcgcacg aggacattga cctgaagaac
     4621 cccggcccca accgcggcgc ggcgggtttt tcggatcagg agcgcggtaa gctgggcgag
     4681 ctgctggcgg cgggcttcac ggattccttc cgccatctgc accccgacgc caccggtatg
     4741 tacagctggt ggagtatgcg cttccgcgcg cgggaacgca acgcgggctg gcgcattgac
     4801 tattttctgg tcagtgaccg cattgccgac aaaatccaga ccgccgcgat cctgatggat
     4861 gtgatgggca gcgaccattg cccggtggag ctggatatca acctgtaaag ccttctccct
     4921 tgggagaagg tggccgcagg ccggatgagg ggcggccttc cggtggctgc ccggtaaaag
     4981 ggcgataacg gcacacctgc ccctcatcag tctgcttcgc agacagcttc ggtctccgcg
     5041 ctaagagccg cctacggcgg ttgcgcttcg acacgcgcct gcgggcgcag cccctcgagg
     5101 gaagccaaag aagaaaactt tttttaaaaa ttcccccgcg cttgcaatat cggcgccggg
     5161 ggattcgttt tagtatacag aaggacaaag caagggaggt gtgggcactg acaaatgaag
     5221 aatttaccgg gctgatgcag caataccaaa agctcatcta cacggtctgc ctgcaatttg
     5281 tgcacgagcc ccacaccgcc gaggacttga cccaggatac cttcctctcc gcgttttcgg
     5341 ctatcgaccg atgcgagccg cagtattaca aacagtggct cgtgcgggtg gcagccaaca
     5401 aatgcaagga tcacctgaaa agcgcctggg tgcgcaaggt ggatgccccg ggcgacgatg
     5461 ccctgccgga gccgcgcggc gcacccggca gcgacgccga cgacccggcg gtaaacctgg
     5521 ccgaccgcgc cggggccgca gagctggaac agctggtgcg gaatctgcgt gagccttacg
     5581 gaaaaatcgc agtcatgtac tttttggagc acaaggaaag ctccgagatt gcaaagctgg
     5641 tgggccgccc gcctgcgacc atcaacagcc agctatggcg cgccaaactg ctgctgcgcc
     5701 agcaaattac cgagaggagg cagaacgaat gagcctttat tttgataacg aaaaactctt
     5761 tgacgccgaa ggccatttga ccgacgaggg cctgtacgct ttgaaggacg gcaccctgga
     5821 cgacttgggt gcgctggagg ctgccgagca cctgagcttc tgcgactact gcctgctgcg
     5881 ctacacagcc atgatcgagg ctgcgcccga ctgcatgaaa cagccgatgc gcgacctgat
     5941 cccgcaggtg caaaacctga tgcggctgcg cagcttccgc attatgacaa accgctatgt
     6001 gtctgcggcg gccgccgtcg tgctggggtt tgtgatgtgg ggtgcggtca gcacattcgg
     6061 cctgccggat gtctccgccc tgaccgccca gcagcccacc accccgcatg agcggttcgg
     6121 caccaaattc agcaccgccg tcaacggctt ttacaagagc ctagacgata cctttaaaaa
     6181 ctttaccctg accgctgaga gcggcctgga acaaatgcga accaaaccga tcggcggcag
     6241 caccgccaag ggagaatgac ctatgaaaaa gaatggattc ctgaccttta tcttcgcctg
     6301 catccccggt gcggggcaga tgtactacgg ctatatgcag cgcggcctgt cgattgcgat
     6361 gatactgata ctctgcgtga tggcggccac cgtcgttgaa ccgctgctgt tcctctgcct
     6421 tgttatctgg atgtacagct tttttgacac ctatgacctc atccgccaca tggcggccgg
     6481 tgagccgaag gaggattccc tgctggttct gggcaattat gaagagatca aaaagctggt
     6541 cccccagcat aacaggctga ttggctgggg tctggtcggc ttcggcgtct gggcactgta
     6601 tgagacattc gtctcaacct ggctctatac gctgctgtgc aatcttgtgg gcgacggcta
     6661 cgcctacgac atcatcaccg gcataccgaa tgttgtcatt gcggcactgc tgattttcgc
     6721 aggcctcaag ctgctgggcc tgcaccccga aacgaagcac ggcgatgacg agctgccgcc
     6781 gtatccgcat gaataacgcc ctgccttccc ccttggggga aggtggcgcc gcagcgccgg
     6841 atgaggggcg gccttacgga cgccgctcgt ctgccttttg ccgcagggta ggggcgggac
     6901 atgtcccgcc cgctgctgac ccaccgccgc tgtgcccgcc atacacgcgc gggccgggca
     6961 tacccggccc ctactgtgca acgaagaata aatgaggtaa tagaatcatg gacgaaaaaa
     7021 ccaccccgaa aaagacacgc cgcgtgggga cgctggcgtt tgcactggtg ctgatcctgg
     7081 ccggtgtgct gctgatgctc taccaaatcg tgccggcgtt caacctgctg caaattgtaa
     7141 agttctcccc ggtcatcctc attgtgctgg gtattgaaat gctcgtgtac agcgccaagc
     7201 ccgatgtgct ggtcaagttt gactggctgg ccatgctggg tacggcgttc accctttgcg
     7261 ttgtcggcgc ggcggcgctg ctgcccctgg ctgtcagtga gtggggccct gcccgcagca
     7321 gcgccataag ccgcatcgag acggaaaagg tggacgccct gtacagtgcc ctgaccgccg
     7381 acccggagct gaaagccaaa acgggctact gcggtgtcaa cgtctggttc aaccacggcg
     7441 cgggcggcag ctacacgctg caaagcggcg acgactgcgt gctgaacgcc accctgaccg
     7501 gcccctacgc cgacgccgag tcctttgccg ccgactgcat aggcattatg cagctggccg
     7561 cggacaagga cctgggcttt acgagctacc acttctccag cgatgaggac accgacgacg
     7621 gcatcagcta ctacctggac tgcgtcgcct cctacccggc ggggctgacc gctgcccagg
     7681 tggcccagcg cgtgacggaa agctaccact atgacggctc cagcttctcc agcgaggccg
     7741 accgtgacga gtacatcaag acccggctgc gcgatgatat cgccgaggaa tacgccaacg
     7801 cccacgacga tgtctacccc gacgacgact atgtagacgc cgaggtcgag cggcggttca
     7861 acgagcaatt tggcatagcc
//
LOCUS       DBMDSQ010000057         7848 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_2496_length_7848_cov_2.016523, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000057 DBMDSQ010000000
VERSION     DBMDSQ010000057.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 7848)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7848)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7848
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2496_length_7848_cov_2.016523"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(207..620)
                     /locus_tag="ACZPAX_03390"
     CDS             complement(207..620)
                     /locus_tag="ACZPAX_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005213770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR1112974.1"
                     /translation="MFCDNLVSLRKLHDLSQEELADRLQVSRQTLSKYETGESLPDIE
                     RCKQLAEIFDVSLDDLVNYDTAATGLNVPPKGRHAFGLVTVGDKGQIVIPAKARKIFN
                     IQPGDKLIVLGDESQGLALLSERDFWAMVQRMRKD"
     gene            complement(610..1692)
                     /locus_tag="ACZPAX_03395"
     CDS             complement(610..1692)
                     /locus_tag="ACZPAX_03395"
                     /inference="COORDINATES: protein motif:HMM:NF013884.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HIR1112975.1"
                     /translation="MRKHYLDNIRWATVLLVVLYHAIFMYNHVVTAGVVGPVTDCRWQ
                     DALQYLLYPWFMVVLFLVSGASARYALDSRSGKVFFAERTRKLLVPSTLGLLVLGWAQ
                     GYFNMAFSHVFENILSGVPAPVLYLILTVSGIGVLWTCHVLWVCALVLLAVRRIECGR
                     LLAKCASLPCWAVAALGVAAWVSAQVLNPPIIQVYRFGIYIFSYLAGYYVFSQPQVMD
                     ALARRAPILCAVAAALGPVYLWHSWGKNYAVAPNVNSPLAIAYGWAACLAVFGGMKRW
                     GDRTGPVAAFLARHSFGLYVCHYLALSAAAYGLVCCLRWRGAVVYLLSAVAAYGGGLA
                     LYAVLRHVPVVRYCVFGIKKEKPHVL"
     gene            complement(2048..3520)
                     /locus_tag="ACZPAX_03400"
     CDS             complement(2048..3520)
                     /locus_tag="ACZPAX_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005941486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HIR1112976.1"
                     /translation="MGKPTGFIEIARQTSLELPPEERIQNFNEFHVPLHTDEQQAQGA
                     RCMDCGVPFCQSGVQLGGMFSGCPLNNLIPEWNDLVYQGKWDLAVHRLIATNRYPEFT
                     SRVCPALCEAACTCGNVTGDPVTVKENERAIVEYGYESGAIHAVPPPARTGKTVAVIG
                     AGPAGLSVADLLNKRGHRVTIYEREDRAGGLLMYGIPNMKLEKWVIDRRVKILQDEGI
                     EFIFNADVGSTVSADELKARYDAVVLCCGAKKARDITAPGRDAQGIYFAVDYLTSVTR
                     SLLDSNFADGKAVNAAGKKVLVIGGGDTGNDCVGTAIRQGCESVMQLEMMPCPPAARA
                     PGNDWPEWPRVLKTDYGQQEAIARFGSDPRVYQTTVKEFYKNEAGQVCGALIAKLKSE
                     VVDGRRQMVPTGEEFTVDCDLVLIAAGFTGCEAYVADAFGVELTKRGTVADVKYATTD
                     PKVFACGDMRRGQSLVVWALREGRDTAAVVDEKLMGYTNL"
     gene            complement(3536..>7848)
                     /gene="gltB"
                     /locus_tag="ACZPAX_03405"
     CDS             complement(3536..>7848)
                     /gene="gltB"
                     /locus_tag="ACZPAX_03405"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HIR1112977.1"
                     /translation="AAELGLEIGKEREYGIGMFFFPQDELKRNQAKKLFEIVCKKEGL
                     PFLGWREVPTCPEVLGHKAIACMPSIWQGFVGKPQRVEAGIAFDRRLYVARRVFEQSS
                     PDTYVCSLSSRTIVYKGMFLVKELRLFYPDLQDTDYESAIGMVHSRFSTNTAPSWNRA
                     HPNRFILHNGEINTIRGNVDTMLAREETIGSNYLKDDMTKVLPIVNQGGSDSAQLDNT
                     LEFMVMNGMDLPLAVMVTIPEPWDNNKSMDPKKRDFYQYYATMLEPWDGPASILFSDG
                     DVMGAVLDRNGLRPSRYYITKDGRLILSSEVGVLDIPADEVVRKDRLRPGKMLLVDTV
                     KGELVDDEKLKADYASRQPYGEWLDRNLVPLSGLKVPNKKVPSYTKDQLVQLQKAFGY
                     RYEDVSTIILPMAKNGGEPAGAMGSDTPLAVLSHTRPSLSEYFKQMFAQVTNPPIDAL
                     REKIVTSTTVYVGAQGNLLEEDADNCKVLKIENPILTETDLLKIKAMDVPGFKVVTLS
                     ICYYKNTDLEKAIDRLFVDVDRAYRDGANILILSDRDIDEYHVAIPSLLAVGAVSKYL
                     VRTRKRTAMALILESGEPRLVHDFATLLGYGAAAINPYLAQETIGGLISDGLLDKDYY
                     AAVEDYNKAVLAGIVKIASKMGISTIQSYAGSQIFEALGISKEVVDKYFTNTVSRVGG
                     ITIQDIQKDLEARHQEAFDPLGLDINRELPSLGAHKFRGGPAAEQHLYNPQTIHLLQQ
                     ACWTGNYDTFKQYTAAAANENGDAMHLRSLLDFNYPEQGVLLDEVESVDSIVKRFKTA
                     AMSYGALSEEAHECMAIAMNRLGGKSNTGEGGEAEDRFGTERNSAIKQVASARFGVTS
                     KYLVSASEIQIKMAQGAKPGEGGQLPGGKVYPWVAKTRNSTTGVGLISPPPHHDIYSI
                     EDLAQLIYDLKCSNRKAAINVKLVSEAGVGTIAAGVAKAGAEVILISGFDGGTGAAPR
                     NSIHNAGLPWELGIAEAHQCLIMNGLRSRVRIEADSKLMSGRDVAIAALLGAEEFGFG
                     TGPLVAMGCVMMRVCNLDTCPMGICTQNPELRKRFKGKPEYIMNFMRFMAEDLREYMA
                     KLGVRTVDELVGRTDLLKVKAVPAGSRASEMDLSALLQNPLVENSNVHFNAKDVYNFQ
                     LEKTPDMKVLMKKFKKNFDSAEPKPSTVTLDVGNTDRAFGTIFGSEITAKFGNTLPED
                     TFHVVCHGYGGQSFGAFIPQGLTLELVGDANDYLGKGLSGGKLVVYPPKDAAFDRSEN
                     IVIGNVALYGATGGKAFINGVAGERFCVRNSGGVAVVEGVGDHGCEYMTGGTVVVLGK
                     TGKNFAAGMSGGIAYVLDEDWDFYQRVNKDMVSLEPVEHKYDVSLLKDLIREHVELTG
                     SPRGKEILDNFGEYLPKFKKVLPHDYDKMLRLIAQMEEKGEDSEQAQIEAFYAMKSAK
                     "
BASE COUNT         1489 a         2364 c         2409 g         1586 t
ORIGIN      
        1 accgcaaccc gacattatcg agcattttgg caacgcagat gccgtgccgc agccgccgga
       61 gcggtgctta agccgtaagg tgggaattgt gcggtgttgc cctaggttgt agggggcgtc
      121 ctcagccacg atcaaatgag cgacgccctg caaggcctgg gggacgtatt ccttgattgg
      181 gtggtgactt cgtacatttt catgggtcaa tcctttcgca ttcgctgtac catcgcccaa
      241 aagtctctct cgctcaacag ggccaggccc tgggattcgt cgcccaggac aatgagctta
      301 tcgccgggct ggatgttgaa aatcttgcgt gccttcgccg gtattacgat ctgcccttta
      361 tcgccgaccg tgaccagccc aaaggcgtgc ctgcccttgg gcggtacgtt cagcccggta
      421 gcggcggtgt catagttgac gagatcgtcc agcgagacgt cgaaaatttc cgccagctgc
      481 ttgcagcgct caatgtcggg caggctttcc ccggtctcgt atttcgagag cgtctggcgg
      541 ctgacctgca ggcggtcagc tagttcttcc tgcgatagat cgtgcaattt gcgcagggag
      601 acaaggttat cacaaaacat gaggtttctc ctttttaatg ccgaaaacac agtaccgcac
      661 caccggcaca tggcgcagca cggcatacag cgccagcccg ccgccgtagg cagctacggc
      721 ggacagcagg tacaccaccg cgccgcgcca gcgcaggcag cacaccagcc cataggccgc
      781 cgccgacagc gccagatagt ggcagacgta cagcccaaaa ctgtgccgcg ccaggaacgc
      841 ggccacaggc ccggtgcggt cgccccagcg cttcatgccg ccaaagacag ccaggcaggc
      901 cgcccagccg taggcgatgg ccagggggct gttcacgttg ggagccacgg cgtagttctt
      961 gccccaactg tgccagaggt aaaccggccc cagcgcggcg gccacagcgc acaaaatcgg
     1021 tgcccggcgg gccagcgcgt ccatgacctg cggctgcgaa aagacgtagt acccagccag
     1081 atagctgaaa atgtagatgc cgaaccgata cacctggatg atgggcgggt tcagcacctg
     1141 ggccgacacc caggcggcca cacccagcgc tgcgacggcc cagcagggga gcgacgcaca
     1201 ttttgccagc agccgcccgc actcgatgcg gcgcacggcc agcagtacca gggcacagac
     1261 ccacagcaca tggcaggtcc agaggacgcc aatacccgat accgtcagga tcaaatagag
     1321 caccggtgcg ggcaccccgg agaggatatt ttcaaagaca tggctgaacg ccatgttgaa
     1381 atacccctgc gcccagccca gcaccagcag tcccagcgtg gaaggaacca gcaatttccg
     1441 cgtgcgctcg gcaaaaaaca ccttgccgct gcggctgtcc agcgcgtacc gtgccgacgc
     1501 accgctgacc agaaacagca cgaccatgaa ccatgggtac agcagatatt gcagcgcgtc
     1561 ctgccagcgg cagtccgtca ccggccccac gacacccgcc gtcacgacat ggttgtacat
     1621 aaaaatggcg tggtacaaaa ccaccagcag cacagttgcc cagcggatgt tgtcgagata
     1681 gtgctttctc atatttgtca cacctttgct atgattttta acaagtatag caaaagcaga
     1741 agaaaatgaa caccaacatg gttttacagg atggcggaga aaatgttaaa aatcgtggca
     1801 ggaggtggtt atagtatagg ggatagggca ggaaattgca agaaaaaagc ccccgcagcg
     1861 tatcgaaagg ccgatacgtt gcggaggctg ctttgcaggg gccgcacatg tgcggcccgc
     1921 ggcgttatgg gagatatgcg tttgcgggaa ggcaggaggg ccgggcatgc ccggccccta
     1981 cggggcggcc catgtatggg ttaaggtttt aggtttcttt gcctactttc ttttcaaaga
     2041 aagtaggtca caggttcgtg taccccatca atttttcatc caccacggcg gcggtgtcgc
     2101 ggccctcgcg cagcgcccac acgaccaggc tctgaccgcg gcgcatatcg ccgcaggcaa
     2161 acaccttcgg gtcggtggtg gcgtatttca catcggccac ggtgccgcgc ttcgtcagct
     2221 caacgccgaa cgcatccgcc acatacgctt cacacccggt aaagcctgcg gcaatcaaga
     2281 ccagatcgca gtccacggtg aactcctcgc cggtgggcac catctggcgt cggccgtcga
     2341 cgacctcact tttcagcttg gcgatcagcg caccgcagac ctggccggcc tcgttcttgt
     2401 agaactcttt gaccgttgtc tggtagacgc ggggatcact gccaaacctg gcgatggcct
     2461 cctgctggcc gtagtcggtt ttcagcacgc ggggccactc cggccaatcg ttgccggggg
     2521 cgcgggcggc gggcgggcag ggcatcattt ctaactgcat gacgctctcg cagccctggc
     2581 gaatggccgt gcccacacag tcgttgccgg tgtcgccgcc gccaatgacc agtaccttct
     2641 tgcctgcggc attcacagcc ttgccgtcgg caaagttcga atccagcaaa ctgcgggtca
     2701 cgctggtcag gtaatccacg gcaaagtaga tgccctgggc gtcgcggccc ggcgcggtga
     2761 tgtcgcgggc tttctttgcc ccgcagcaga gcactacggc gtcatagcgg gctttcagtt
     2821 cgtcggcgct gacggtgctg ccgacatcgg cattaaagat aaactctatg ccctcgtcct
     2881 gcaaaatctt cacgcggcgg tcaatgaccc acttttccag cttcatgttt gggatgccgt
     2941 acatcagcag gccgcccgcg cggtcctcgc gctcgtaaat ggtcacgcgg tggccgcgct
     3001 tgttcaaaag atccgccacg gacaggcccg caggccctgc gccaatgacc gccacggtct
     3061 tgccggtgcg ggccgggggc ggcacagcat ggatcgcgcc gctctcgtag ccgtattcca
     3121 cgatggcacg ctcgttctcc ttgaccgtga ccgggtcgcc ggtgacattg ccgcaggtgc
     3181 aggcggcttc gcacagggcg gggcagaccc ggctggtgaa ctctgggtag cggttcgtgg
     3241 caatcaggcg gtgtacggcc agatcccact tgccctggta gaccaggtcg ttccactcag
     3301 ggatcaggtt attcaacggg cagccgctga acataccgcc cagctggaca ccgctttggc
     3361 agaaggggac gccgcagtcc atgcaacgtg cgccctgggc ctgctgctcg tcggtatgca
     3421 gcgggacgtg aaactcgtta aaattctgga tgcgctcctc gggcggcagc tctaaactgg
     3481 tctgccgcgc gatctcgata aatcctgtcg gtttgcccat gatactctgc ctcccttact
     3541 ttgcgctttt catggcgtag aacgcctcga tttgggcctg ctcgctgtcc tcgcccttct
     3601 cttccatctg ggcaatcagg cgcagcatct tgtcgtagtc gtggggcagg accttcttga
     3661 actttggcag atactcgccg aagttgtcga ggatttcctt gccgcgcggc gacccggtca
     3721 gttcgacgtg ctcacggatc aggtctttta acagcgatac atcgtactta tgctccacgg
     3781 gctccaggct taccatgtct ttgttgacgc gctggtagaa gtcccagtcc tcatcgagga
     3841 cataggcaat gccgccgctc atgccggctg caaagttttt gccggtcttg ccaagcacga
     3901 ctaccgtgcc gccggtcatg tactcgcagc cgtggtcgcc cacgccctca acgacggcca
     3961 cgccgccgga attgcgcacg cagaagcgct cgcccgcaac accgttgatg aacgccttgc
     4021 cgccggttgc gccgtagagc gccacgttgc cgatgacaat gttttcgctg cggtcaaagg
     4081 cggcatcctt cggcggatag acgacgagct tgccgccgga caggcccttg ccaaggtagt
     4141 cgttggcgtc gccgaccagt tccagcgtca ggccctgggg gatgaacgcg ccaaagctct
     4201 ggccgccgta gccgtggcag acgacatgga aggtatcttc cggtaaggtg ttgccgaact
     4261 tggcggtgat ttcactgccg aagatcgtgc cgaaggcgcg gtcggtgttg ccgacatcca
     4321 gcgtaacggt gctgggcttc ggctcggcgg aatcgaaatt cttcttgaac ttcttcatca
     4381 gcaccttcat gtcgggcgtc ttttccagtt ggaagttgta gacatccttc gcgttgaaat
     4441 gtacgttgga gttctcgacc aaggggttct gcaagagggc cgaaagatcc atctcgctgg
     4501 cgcgggaacc tgcgggcacg gccttgacct tcaacaggtc ggtgcggccg acgagttcat
     4561 caacggtgcg cacgccgagc tttgccatat actcacgcag atcctcggcc atgaagcgca
     4621 tgaagttcat gatgtactcg ggcttgccct tgaagcgctt gcgcagctcg gggttctggg
     4681 tgcagatgcc catggggcag gtgtccagat tgcagacgcg catcatgacg cagcccatgg
     4741 ccaccagtgg cccggtgccg aaaccaaatt cctcggcacc cagcagagcg gcgatggcaa
     4801 cgtcgcggcc gctcatcagc ttgctgtcgg cctcgatgcg gacgcggctg cgcaggccgt
     4861 tcatgatgag gcactggtgc gcctcggcaa tgcccagctc ccaaggcaag cctgcgttgt
     4921 gaatggagtt gcggggcgct gcgccggtgc cgccgtcaaa gccggaaatc aggatgacct
     4981 ccgcgcctgc cttggcaacg cctgcggcga tcgtgccgac accggcctcg ctgaccagct
     5041 tgacattgat agcggccttg cggttggagc acttcaggtc gtaaatcagc tgggccagat
     5101 cctcaatgga atagatatcg tggtgcggcg ggggagagat aaggcccaca ccagtggtgg
     5161 agttgcgggt cttagcgacc caggggtaga ccttgccgcc gggcagctgg ccgccctcgc
     5221 cgggcttcgc accctgggcc atcttgattt ggatttcgct ggcggagacg agatacttgc
     5281 tcgtcacgcc gaagcgggcg gacgctacct gcttgatggc ggagttgcgc tcggtaccga
     5341 agcggtcctc ggcctcgccg ccctcgccgg tgttggattt accgcccagg cggttcatag
     5401 cgatggccat gcattcgtgg gcttcctcag acagtgcacc gtagctcatg gcggcggtct
     5461 tgaagcgctt cacgatggaa tcgacgctct cgacctcgtc caggagcaca ccctgttcgg
     5521 ggtagttgaa atccagcagg ctgcgcaggt gcatggcgtc gccgttctca ttggcggcag
     5581 ctgcggtgta ctgcttgaac gtgtcgtaat tgccggtcca gcaggcctgc tgcagcaggt
     5641 ggatggtctg cgggttgtag agatgctgct cggctgcggg gccgccgcgg aacttgtgtg
     5701 cacccaggct gggcagttcg cggttgatat ccaggcccag ggggtcaaag gcttcctgat
     5761 ggcgggcttc cagatccttc tggatatcct ggatcgtgat gccgcccacg cgggagacgg
     5821 tgttcgtaaa gtatttgtcc acgacctctt ttgagatgcc cagcgcttca aaaatctggc
     5881 tgcccgcgta ggactggatg gtggaaatgc ccatcttgga cgcgattttg acaatgcccg
     5941 cgaggacggc cttgttgtaa tcctcgacgg cggcatagta atctttgtcg agcaggccgt
     6001 cggaaatcag cccgccgatg gtctcctgcg ccaggtacgg gttgatggcc gctgcgccgt
     6061 agcccaagag cgttgcgaag tcatgcacca ggcgcggctc gccgctttcc agaatcagcg
     6121 ccatagccgt gcgcttgcgg gtgcggacca ggtacttgct gactgcgccc acagccaaaa
     6181 ggctggggat ggcgacgtgg tactcgtcaa tgtcgcggtc ggacaaaatc aggatgttgg
     6241 cgccgtcgcg gtaggcgcgg tccacatcga caaacaggcg gtcaatggcc ttttccaggt
     6301 cagtgttctt gtagtagcag atagacagcg tcacgacctt gaagccgggc acatccatcg
     6361 ccttgatttt gagcagatca gtctcggtca gaatcgggtt ctcgattttc agcaccttgc
     6421 agttgtcggc gtcctcttcc agcaaattgc cctgggcacc gacatatacg gtggtggaag
     6481 tgacgatctt ctcgcgcagg gcatcaattg gcgggttcgt gacctgggcg aacatctgct
     6541 tgaagtactc gctcaaactc gggcgcgtgt ggctcaaaac ggccagcggg gtatcgctgc
     6601 ccatggcccc ggcaggttcg ccgccgttct tcgccatcgg cagaatgatc gtagatacgt
     6661 cctcataccg gtagccgaag gccttttgca gctggacgag ctggtctttc gtgtagctgg
     6721 gcaccttctt gttgggcacc ttcaggccgg acagcggaac caggttgcgg tccagccact
     6781 cgccgtaggg ctggcggctg gcgtagtcgg ccttcagctt ttcgtcgtcc accagctcgc
     6841 ccttgacggt gtccaccaac agcatcttgc cggggcgcag gcggtccttg cgcacgacct
     6901 cgtcagcagg aatatccagc acgccgacct cgctggacaa aatcaggcgg ccgtccttcg
     6961 tgatgtagta ccggctggga cgcaggccgt tacggtccag caccgcgccc atgacgtcgc
     7021 cgtcgctgaa caggatactg gcggggccgt cccacggctc cagcatcgtg gcgtaatact
     7081 ggtagaaatc acgcttcttc ggatccatgg atttgttgtt gtcccaaggc tcggggatgg
     7141 tcaccatgac ggccagcggt agatccatgc cgttcatgac cataaattcc agggtgttgt
     7201 ccagctgggc agagtcagag cctccctggt tgacgatggg cagaaccttc gtcatatcgt
     7261 cctttaagta gttggagccg atggtctcct cgcgggccag catcgtgtcc acgttgccgc
     7321 ggatggtgtt gatctcgccg ttgtgcagga taaagcggtt ggggtgggcg cggttccaac
     7381 tgggggcggt gttggtggag aagcggctgt gtaccatgcc gatggcggac tcgtagtcgg
     7441 tgtcctgcaa gtcgggatag aacaggcgca gctccttgac gaggaacata cctttatata
     7501 cgatggtccg gctggacagg ctgcaaacgt aggtgtcggg gctggactgc tcaaacacgc
     7561 ggcgggcgac atacaggcgg cggtcaaagg caatgcccgc ctctacgcgc tgcggcttgc
     7621 ccacaaagcc ctgccagatg ctaggcatac aggcaatggc cttgtggccc agcacctcgg
     7681 ggcaggtggg gacttcgcgc cagcctaaga agggaagccc ctcttttttg cagacgatct
     7741 caaacaactt tttggcttgg ttgcgcttga gttcatcctg cgggaaaaag aacataccaa
     7801 tgccgtattc ccgctctttg ccgatttcca ggcccagctc ggccgccg
//
LOCUS       DBMDSQ010000058         7827 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_2506_length_7827_cov_1.484986, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000058 DBMDSQ010000000
VERSION     DBMDSQ010000058.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 7827)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7827)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7827
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2506_length_7827_cov_1.484986"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..729)
                     /locus_tag="ACZPAX_03410"
     CDS             complement(<1..729)
                     /locus_tag="ACZPAX_03410"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HIR1112978.1"
                     /translation="MKTQVLPITPESVALAAKLLQQGELVALPTETVYGIAADARNGA
                     AVKKIFEAKGRPQDNPLIVHIYGMEMLNGIVSEVPERAKKLAAAFWPGPLTMVMPRGP
                     EVSDVTCAGLDTVGVRMPSHPVVQQVIKESGVAFAAPSANLSGKPSPTNAPDTLADMD
                     GRLPLILDGGESKVGVESTVVAVTGEHPMLLRPGYVTKEQMEAVLGEEVLVSPAILEK
                     LKDGEVARSPGMKYKHYAPKAQVTI"
     gene            complement(731..1819)
                     /gene="prfA"
                     /locus_tag="ACZPAX_03415"
     CDS             complement(731..1819)
                     /gene="prfA"
                     /locus_tag="ACZPAX_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HIR1112979.1"
                     /translation="MQNFTELYEVERRYEELAHKMSTPDAAANADEYARMMRDYKELT
                     SLIEEYRRYTDLQQQEKDAKEMLAAESDPAFAAMVQQELCEIARNLAQSEENLRLLLI
                     PKDADDEKSVILEIRAGAGGEEAALFAHSLWRMYTMYAARRGWQCETISANETELGGV
                     KEIVFSVEGPDVYSRLKFESGVHRVQRVPETETQGRIHTSTVTVAVMPEAEEVELEIN
                     PKDLRIDTFRSSGAGGQHINKTSSAIRVTHLPTGMVVECQDQRSQRENKDRALKVLRS
                     RLLQQKQDAYDEEYNARRQSQVGSGDRSEKIRTYNFPQDRVTDHRIGLTLRNLQGVLG
                     GDLDRVVEPLILADREEKLKQSSTEEGK"
     gene            complement(1985..2173)
                     /locus_tag="ACZPAX_03420"
     CDS             complement(1985..2173)
                     /locus_tag="ACZPAX_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HIR1112980.1"
                     /translation="MDVQVGDVIQTKKPHPCGANRFDVLRVGMDFKIKCQGCGHEIML
                     PRAKIERNIKKVLRTGEQ"
     gene            2309..3217
                     /locus_tag="ACZPAX_03425"
     CDS             2309..3217
                     /locus_tag="ACZPAX_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HIR1112981.1"
                     /translation="MLEFRAEGLCRNANHLNREELSRCMANGEVLQSTALAYDTERRL
                     RFELGGVRGIMPFADCVDAAPGETVKDIAVLTRVGRPTCFVIMGTEFDENGEEYYLLS
                     RAEAQRRCRAQYLDTLEAGSVIPCTVTHIENFGAFCDIGCGIAALLPIDCLSVSRIAS
                     PADRVQVGQQLLCAIKNRDAQGRIVLTLRELLGTWSENAACFAAGETVVGIVRSVEDY
                     GVFIEIAPNLAGLAEADSTLRPGQAVSVYIKNILPDKMKIKLVVVNKNLNQPLRFEPH
                     YFVTRGRLKRWTYSTPQSRKQIETVF"
     gene            3318..3893
                     /locus_tag="ACZPAX_03430"
     CDS             3318..3893
                     /locus_tag="ACZPAX_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4364 family protein"
                     /protein_id="HIR1112982.1"
                     /translation="MAEAFTAGVRPGGLTDDTQIRMLLCYLVKVAGPVKHETLQGALL
                     QEQLVNYFEFADALAEVEKQKLVTVDSVGRYTITRKGATVADTLAYDLPRTVRESAIR
                     AVMQIRSWNHRAASNRAVVQETDGKFSVVCSIGDMGSDVFRMELAMPDKLTAEMIKNN
                     FIAHGSDIYPKLMDMLTQPGSEDDRPPAALQ"
     gene            4159..4530
                     /locus_tag="ACZPAX_03435"
     CDS             4159..4530
                     /locus_tag="ACZPAX_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR1112983.1"
                     /translation="MQIYLSNSGQEPIYAQITRQIKQQILSGALHPGDALPSIRLLAK
                     ELRISVITTKRAYEDLERDGFILPQPGRGSFVAEQNPALLREEHLKKVEDCLQGAVDA
                     ARLGGIGYDEVAETLRLLWEG"
     gene            4534..5394
                     /locus_tag="ACZPAX_03440"
     CDS             4534..5394
                     /locus_tag="ACZPAX_03440"
                     /inference="COORDINATES: protein motif:HMM:NF012235.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1112984.1"
                     /translation="MAANAAALRGITKQYKDFTLGPVDMTVPAGSIVGLIGENGAGKT
                     TLLKILCGVNRADDGTVDLLGGSPADAATRAKIGVVFEDAFFYGSFNAAQVARSLAGM
                     YGARWNADSFSAYLRRFGLDAGKKLKEYSRGMRLKLSLAAALAHDPELLVLDEATAGL
                     DPVVRGELMDLFLEFIQDERHSIIMSSHITADLEQVADSIAYLHNGQLLFHENKDDLL
                     QEYGVLHCGEDVLTSLPAGLVVFTRRGAYGCESLVRERRTVQELLPEAVCDAARLDDI
                     MRFYSGRDAQ"
     gene            5391..6053
                     /locus_tag="ACZPAX_03445"
     CDS             5391..6053
                     /locus_tag="ACZPAX_03445"
                     /inference="COORDINATES: protein motif:HMM:NF024741.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HIR1112985.1"
                     /translation="MKGLLYKEWAILTSSYKQSVFFIAILYGGISILTGQTGMAYALL
                     VVFSILITSTISFDENSHWDIYARTLPVTPAQLVGSKYLFGLCGLALGTVCTVLIVAL
                     NNVLPPLLFRHAAYKVPPLECLAALLACGSMALLLVALLLPLSYRFNSVKARSWLFLI
                     IGVLGGCIGLVASVSPDLMQLFNASDEVLLTGLVAAFVGLLAVYFVSYKVCVGIYKRK
                     EY"
     gene            complement(6207..6926)
                     /locus_tag="ACZPAX_03450"
     CDS             complement(6207..6926)
                     /locus_tag="ACZPAX_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1112986.1"
                     /translation="MGEMLSVKNINVYYGSIHAIKDVSFHVNEGEIVTLIGANGAGKT
                     TTMHAISGLLKLQSGEINYCGQTISKMEAHKIIRLGLAQVPEGRRVFSGLTVQQNLQM
                     GAYTRRDGKEAIQNDFDMVFDLLPRLKERRNQPAGTLSGGEQQMLAMGRALMCKPKML
                     LLDEPSMGLSPLLVKEIFKIIRQVNRNGVTVLLVEQNAKMALEIANRAYVLETGTIKM
                     EGEATELANNIEVRKAYLGCE"
     gene            complement(6929..7690)
                     /locus_tag="ACZPAX_03455"
     CDS             complement(6929..7690)
                     /locus_tag="ACZPAX_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1112987.1"
                     /translation="MALLEVSNLGIAFGGLQAVAQLDLSIEKGHLYGLIGPNGAGKTT
                     VFNMLTGVYKPTEGNIILDGKDITGQNPELISKSGIARTFQNIRLFNEMTVLDNVKVG
                     LHNQIKYGMWTGILRLPAYKEKEHEMNREAMKLLKIFDLDREAHYKASQLPYGKQRKL
                     EIARALATNPKLLLLDEPAAGMNPNETEELMHTIRSVRDQFGVAILLIEHDMNFVMGI
                     CEEITVLDYGRVIARGDGKAIRNNPKVIAAYLGGD"
BASE COUNT         1458 a         2276 c         2339 g         1754 t
ORIGIN      
        1 aatcgtgacc tgtgccttgg gggcgtagtg cttgtacttc ataccgggcg aacgtgcaac
       61 ttcgccgtcc ttgagctttt cgagaatggc cgggctgacg aggacttcct cgccgaggac
      121 agcttccatc tgctccttcg tgacatagcc ggggcgcaaa agcatcgggt gctcccccgt
      181 cacggccaca acggtggact ccacgccgac cttcgactcg ccgccatcaa gaatcagcgg
      241 cagacggccg tccatatcgg ccagcgtgtc gggagcgttc gtggggctgg gcttgccgga
      301 aaggttcgcc gagggcgcgg cgaaggcgac gccgctttct ttaatcacct gctgcacgac
      361 cgggtgggac ggcatacgca cgcctacggt gtccagccct gcgcaggtca cgtcgctgac
      421 ctcggggccg cggggcatga ccatcgtaag tgggccgggc caaaaggcgg ccgccagctt
      481 tttggcgcgt tcgggcactt cggagacgat gccgttcagc atttccatcc catagatatg
      541 tacgatgagg gggttatcct gcgggcggcc ctttgcttca aatatttttt tcaccgctgc
      601 gccgttgcgg gcgtcggcgg cgatgccgta gaccgtctcg gtgggcaggg cgaccagctc
      661 gccctgctgc aggagcttgg cggccagtgc cacgctttcc ggcgtgatgg gcaaaacctg
      721 tgttttcata ttactttcct tcttctgtgc tgctctgttt cagcttttct tcccggtcgg
      781 ccagaatcag cggctcgacc acgcggtcca agtcgccgcc cagcacgcct tgcaggttgc
      841 gcagcgttaa accaatgcgg tggtcggtca cacggtcctg cgggaagttg taggtgcgta
      901 tcttttcgct gcggtcgccg ctgcccacct ggctttggcg gcgggcgttg tactcctcgt
      961 cgtaggcgtc ctgcttttgc tgtaaaaggc ggctgcgcag gactttcaag gcgcggtcct
     1021 tgttctcccg ctggctgcgc tgatcctgac attcgaccac catgccggtg ggcaggtggg
     1081 tcacgcggat ggcggacgag gtcttgttga tgtgctggcc tcccgcgccc gaggagcgga
     1141 aggtgtcgat gcgcaggtct ttcgggttga tctccagctc gacctcctcg gcttcgggca
     1201 tgacggcgac tgtcaccgtt gaggtgtgga tgcgcccttg ggtctcggtt tcgggcacgc
     1261 gctggacgcg gtggacgccg ctctcaaatt tcaggcggct gtacacgtcg gggccttcga
     1321 ccgagaaaac aatttccttc acaccgccga gttctgtctc attggcgctg atggtctcgc
     1381 actgccagcc gcgcctagca gcgtacatcg tgtacatgcg ccacaggcta tgggcaaaca
     1441 aggccgcttc ctcaccgccg gcaccggcgc gaatttccaa aataacgctt ttttcgtcgt
     1501 cggcgtcctt ggggataagc agcaaacgca aattctcttc gctctgtgcc agattccggg
     1561 caatttcgca gagttcctgc tgtaccatag cagcaaacgc cggatccgat tcggcagcca
     1621 gcatttcttt ggcgtccttc tcctgctgct gcaggtcggt ataacggcgg tattcttcga
     1681 tcaaggaggt aagttctttg taatcacgca tcatccgcgc atactcgtcg gcgttggcgg
     1741 ccgcgtcggg cgtggacatt ttgtgggcaa gctcctcata gcggcgctcg acctcataaa
     1801 gttcagtaaa attctgcatg ggttctcctt tgcacatggt cattttgtag gggcaaacag
     1861 tgtttgcccg cggagcttta cggctgccgc caagttcccg ggtcgatgca agcatcgccc
     1921 cctacacgca ggggcggatt ccatacccgc ccgaaaaagt tgccaatacg ttgtgcaaag
     1981 cccgctactg ctcgcccgtg cgcagaactt ttttgatgtt gcgctcgatc ttggcgcggg
     2041 gcagcataat ttcatgcccg cagccctggc acttgatttt gaaatccatg cccacacgca
     2101 gtacatcgaa acggttcgca ccgcaggggt gcggcttctt ggtctggatc acgtccccga
     2161 cctgtacgtc catacggcac accgcctttc ttaaaaaata tcagttctat tatagtacag
     2221 ccggtattgt tttgcaaaca tcccgcataa aagtttagca agttatcaca aacacaccct
     2281 aaattttaga agaaatgagg aaccgaaaat gttggaattc agagctgaag gtctttgccg
     2341 caacgcgaac cacctgaacc gcgaggaact gagccgctgc atggcgaacg gcgaagtgct
     2401 gcaaagcacc gcccttgcct acgataccga gcgtcggctg cggtttgagc tgggcggtgt
     2461 gcggggcatt atgccctttg cggattgtgt ggacgctgcc cccggcgaga ccgtgaagga
     2521 cattgccgtg ctgacccggg tgggccgccc gacctgcttt gtcatcatgg gaaccgagtt
     2581 tgacgaaaac ggcgaggaat attacctgct ctcccgcgcg gaggcccagc gccgctgccg
     2641 ggcacagtac ctggacacgc tggaagcggg cagcgtcatt ccctgcaccg tgacccacat
     2701 tgaaaatttc ggcgcgttct gtgatatcgg ctgcggcatt gcggcgcttt tgcccatcga
     2761 ctgcctttcg gtcagccgta ttgcctcccc cgccgaccga gtacaggtgg gccagcagct
     2821 tttgtgcgcc atcaagaacc gcgatgcgca ggggcgcatc gtcctgaccc tgcgggagct
     2881 tttgggcacc tggagcgaga acgccgcctg ctttgccgcc ggggaaacgg tggtcggcat
     2941 tgtgcgcagc gttgaggatt acggcgtttt tatcgagata gcgccgaacc ttgcgggtct
     3001 ggccgaggca gattccaccc tgcgccccgg gcaggccgtg agcgtgtaca tcaagaacat
     3061 tctgccggat aagatgaaaa tcaagcttgt ggtggtaaac aaaaatctga accagcccct
     3121 acggtttgag ccgcactatt ttgtgacgcg cgggcggctt aagcggtgga cttactcgac
     3181 cccgcagagc cgcaaacaga ttgaaacggt cttttagctg tccgattttt gtcactttgg
     3241 ctttttcgca tcttgccaca gcccggccgg gcgtgtataa tattggtaaa cgaaaatttc
     3301 ttgaggaagt gatcccgatg gcagaagcat ttaccgccgg tgtgcgcccc ggcggcctta
     3361 cagacgacac gcagatccgt atgttgctct gctaccttgt caaagtggca ggcccggtaa
     3421 agcacgaaac cctgcagggc gcgcttttgc aggagcagct tgtcaactat tttgagtttg
     3481 ccgatgcgct ggctgaggtg gaaaaacaga agcttgtcac cgtggacagt gtgggccggt
     3541 ataccatcac ccgcaagggc gcaaccgtgg ccgacacact ggcctacgac ctgccccgca
     3601 ccgtgcggga gagtgccatc cgcgccgtta tgcagatccg cagctggaac caccgcgccg
     3661 cctccaaccg cgccgttgtg caggaaacgg acggcaagtt cagcgttgtc tgctccatcg
     3721 gcgatatggg cagcgatgtg ttccgcatgg agctggccat gcccgacaag ctgaccgccg
     3781 agatgatcaa aaacaacttt atcgcccacg gcagcgacat ctaccccaag ctgatggata
     3841 tgctgaccca gcccggcagt gaggatgaca gaccgccggc agcgctgcaa taacaccttc
     3901 ccccacaggg ggaaggtggc gcgaagcgcc ggatgagggg cggcgttatc actattaccc
     3961 ataaatgggt cgtaacggca agtccacccc tcatcagtca cctgcggtga cagcttcccc
     4021 ctctggggaa gcctttctcc cctgcaccca tacgggtgcg ggggattttt ttgtgccatc
     4081 ccccttgaca actgtgtata gtgtgtatat actgtaaata tcgaatatgc acagttcgag
     4141 atgaaggagc ttcctaccat gcagatttat ctttccaatt ccgggcagga gccgatctac
     4201 gcgcagatca cgcggcagat caagcagcag atcctgtccg gtgcgctgca ccccggggac
     4261 gcgctgccca gcatccgcct gttggcgaag gagctgcgca tcagcgtcat cacgacaaag
     4321 cgtgcgtatg aggatttgga gcgcgacggc tttatcctgc cccagccggg ccggggcagc
     4381 tttgtcgccg agcaaaatcc cgctctgctg cgtgaagaac acttaaaaaa agtcgaggac
     4441 tgcctgcagg gcgcggtgga cgccgcgcgg ctgggcggta tcggctacga tgaggttgca
     4501 gagaccctgc gccttttgtg ggagggctga cctatggcag caaacgccgc cgcactgcgc
     4561 ggcatcacga agcagtacaa agactttacc cttgggccgg ttgatatgac cgtgcccgcg
     4621 ggcagcatcg tcgggctgat cggcgagaac ggcgcgggca agacgacgct tttgaaaatc
     4681 ctctgcggcg tcaaccgggc ggatgacggc acggtcgacc tgctgggcgg ctcccccgcc
     4741 gatgccgcca ctcgcgctaa gattggcgtt gtgtttgaag atgccttttt ctacgggagc
     4801 ttcaacgccg cgcaggtcgc gcgcagtctg gccggaatgt acggtgcgcg gtggaatgcg
     4861 gacagctttt ccgcctatct gcgccgcttt ggtctggacg caggcaagaa gctcaaagag
     4921 tacagccgcg gcatgcggtt aaaactgagt ctggctgcgg cgctggccca tgaccccgag
     4981 cttctggtgc tggacgaggc cacggcgggg cttgaccctg tggtgcgcgg tgagctgatg
     5041 gatctgtttc tggagttcat tcaggatgag cggcacagca tcatcatgag cagccatatc
     5101 acggccgatt tggagcaggt ggcagattct atcgcctacc tgcacaatgg gcagctgctg
     5161 ttccatgaga acaaggatga ccttttgcag gaatacggcg tgctgcactg cggcgaggat
     5221 gtactgactt ccctgcccgc cgggctggtg gtgttcacca ggcgcggggc ctatgggtgt
     5281 gaaagtctgg tcagggaacg gcgcaccgtg caggagcttc tgccggaggc cgtctgcgac
     5341 gcagcaaggc tcgatgatat tatgcggttt tacagcggga gggatgcaca atgaaagggc
     5401 ttttgtataa agaatgggcc attctgacaa gttcctacaa acagtctgta ttttttatcg
     5461 cgatcctcta cggcggcatc agcattctga ccgggcagac cgggatggcc tatgcattgc
     5521 tcgttgtatt ttccattctg atcacctcca caatttcgtt tgacgagaac tcccattggg
     5581 acatctacgc ccgcacgctg cccgtaacac ccgcgcagct tgtgggcagc aaatacctgt
     5641 ttgggctgtg cgggctggcg ctgggcacag tgtgtacggt gctgatcgtg gcgctgaaca
     5701 atgtgctgcc gcccctgctg ttccggcacg ccgcgtataa agtaccgcca ctcgagtgtc
     5761 tggcagccct gctggcctgc ggcagcatgg cgctgctgtt ggtcgcgctt ttgctgccgc
     5821 tgtcctatcg gttcaacagt gtcaaggccc gcagctggtt gtttctgatc atcggtgtgc
     5881 tcggcggttg tattgggctg gtcgcttccg tctcgcccga tttgatgcag ctgttcaatg
     5941 cctccgacga agtgctgctg acggggctgg ttgcagcgtt tgtggggctg ctggcggtgt
     6001 attttgtgag ctataaggta tgcgtgggga tttataagcg gaaggaatac tgagttcctg
     6061 ccgcaatgtg cccgggcgga tatagaatcc gcccctacgt gtgtggacgg ctcccctgcg
     6121 ggcgagcaat gctcgcccct acaagggaga ctttataaca aaaaatcctg tgtccttttg
     6181 cggacacagg attttttagt atgcacttat tcgcagccca gatacgcctt gcggacctcg
     6241 atgttgttgg caagctcggt ggcttcgccc tccatcttga tggtgccggt ttccagaaca
     6301 taggcgcggt tggcgatctc cagcgccatc ttggcgttct gctcgaccag cagcacggtc
     6361 acaccgttgc ggttgacctg acggatgatc ttgaagatct ccttgaccag cagcggagac
     6421 agacccatgg acggctcatc cagcaacagc atcttcggct tgcacatcag ggcgcggccc
     6481 atggccagca tctgctgctc accgccggaa agcgtaccgg cgggctggtt gcggcgctcc
     6541 ttcaggcgcg gcagcaggtc aaacaccatg tcgaaatcgt tctggatggc ctccttgccg
     6601 tcacggcggg tgtaggcacc catctgcagg ttctgctgca cggtcaggcc gctgaataca
     6661 cggcggccct ccggcacctg tgccaagccc aggcggatga tcttgtgcgc ctccatcttg
     6721 ctgatggtct ggccgcaata gttgatctca ccgctctgca gcttcagcag gccgctgatt
     6781 gcgtgcatcg tggttgtctt gccggcgccg ttggcaccga tcagggtgac gatctcgccc
     6841 tcattgacat ggaaggacac atccttgatg gcgtggatcg agccgtagta aacattgata
     6901 tttttgacag aaagcatctc gcccatcgtc agtcacctcc cagatatgcc gcaatgacct
     6961 tcgggttgtt gcggatggcc ttgccgtcac cgcgggcgat cacacggccg taatccagca
     7021 cagtgatctc ctcgcagatg cccatgacaa agttcatatc atgctcgatg agcaggatgg
     7081 ccacaccgaa ctgatcacgc acgctgcgga tggtgtgcat cagctcctcg gtctcgttgg
     7141 ggttcatgcc ggcggccggc tcgtccagca gcagcagctt cgggtttgta gccagcgcgc
     7201 gggcgatctc cagcttgcgc tgcttgccgt agggcagctg gctggccttg taatgtgcct
     7261 ccctgtcaag gtcaaagatt ttgagcagct tcattgcctc gcggttcatc tcatgctctt
     7321 tttccttgta ggcgggcagg cgcaggatac cggtccacat accgtatttg atctggttgt
     7381 gcaaaccgac cttgacattg tccagcaccg tcatctcatt gaacagacgg atattctgga
     7441 aggtgcgggc aatgccggac ttgctgataa gctcggggtt ctggccggtg atgtccttgc
     7501 cgtccagaat gatgttgccc tcggtgggct tgtaaacgcc ggtcagcata ttaaagaccg
     7561 tggttttgcc cgcgccgttg gggccgatca ggccgtacag gtggcccttt tcaatcgaga
     7621 ggtcaagctg cgcaacggcc tgcaggccgc cgaacgcaat acccaggtta ctgacttcca
     7681 gcagtgccat ttacgccacc ccctttttct cttttgcgtc cttcttgtcc gcgccttcct
     7741 tgcggaagcg ggcggtcagc cgcttgcgca aggcgatcat ttcaggactc tggttgaaga
     7801 tcatcacaac gatcagcacg atcgcat
//
LOCUS       DBMDSQ010000059         7816 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_2510_length_7816_cov_1.766170, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000059 DBMDSQ010000000
VERSION     DBMDSQ010000059.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 7816)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7816)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7816
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2510_length_7816_cov_1.766170"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..484
                     /locus_tag="ACZPAX_03460"
     CDS             <1..484
                     /locus_tag="ACZPAX_03460"
                     /EC_number="3.2.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033958 - DNA-deoxyinosine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-deoxyinosine glycosylase"
                     /protein_id="HIR1112988.1"
                     /translation="HGARANALILGSFPSPKSREQGFFYGHKQNRFWPMIAAVTGEPV
                     PDWADIEAKKAIILNHGLAVWDTIGACDIKGASDASIRNAVPNDVAALIRKLGVQAVF
                     CNGAASGRIYAKYAEPLTHLSATVLPSTSPANAAWSPARLQATWGAALEPFLQLTGCK
                     "
     gene            534..1163
                     /locus_tag="ACZPAX_03465"
     CDS             534..1163
                     /locus_tag="ACZPAX_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HIR1112989.1"
                     /translation="MTNWLEKTKRPVLPIYLAALVWPIMALVLPIYTLGGLIATAVIS
                     AGVYIAGTKLCPVRVVRVPVAYATGSEDVDALLNGVTANLDALHKLNDEIPDKQLSDA
                     MDRMEKAGRSIADTVARTPDKARNIDRFARYYLPEMVKLMGAYAELEKQGVKGENADQ
                     IEADLRRNADTTAAAFENLLDALYSAEAMDISTDIEVLDSILKSQNLTK"
     gene            1191..2375
                     /locus_tag="ACZPAX_03470"
     CDS             1191..2375
                     /locus_tag="ACZPAX_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005931526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HIR1112990.1"
                     /translation="MADLNNELDLNAPVAPTLTLDSAPAAPTLTLDPAADEKIVEESK
                     KATPVQVEDTPLSPEEQKMVNDFAEKIDLTNSQMVLQYGAASQKKLSDFSETALSRVK
                     TQDMGETGELITGLIAELQGFDANAEQPKGILGFFKKASNSIEQLKTRYDSADKNVER
                     IKAKLEDHQVVLMKDIAMLDKMYEYNLVYFKELTMYILAGKKKLADVRANELKAAQEK
                     AQRTQLPEDAQAARDLADMCDRFEKKLYDLELTRNVSIQMGPQIRLIQSNDTMMAEKI
                     QTTIVNTIPLWKNQMVLALGIAHSQQAMAAERAVTDATNELLKKNAATLKQGTIDIAK
                     ESERGIVDIETLQQTNKQLIETLDELNKIRADGKVKRANAEQELGRIEGELRQKMLEI
                     NG"
     gene            2628..3263
                     /locus_tag="ACZPAX_03475"
     CDS             2628..3263
                     /locus_tag="ACZPAX_03475"
                     /inference="COORDINATES: protein motif:HMM:NF015944.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HIR1112991.1"
                     /translation="MQLEQQETFTIHGPQLEVLGEVESKLPAPAKKRSTASVVLVALV
                     LVSVFGIGGAKLKGKYNDTAKLYTAIDKYNNSIQTDFGTQVDAAANLIKQVGYLENAP
                     TEALQDARVALEVWNNTAADPAAQYNANRDLYNAVDALYAAVNNKVDASTQQAINNQY
                     NAFVSAQATIERAAAAYNQSVESYETTTAQFPANVISGIWGVREISQFAPQ"
     gene            3286..4296
                     /locus_tag="ACZPAX_03480"
     CDS             3286..4296
                     /locus_tag="ACZPAX_03480"
                     /inference="COORDINATES: protein motif:HMM:NF016424.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HIR1112992.1"
                     /translation="MKLKTFAKRAAAAGAVAAALVLTALPALAAPAVDVDAKNPVADH
                     ANILSADTEEYIYAISSQLSDACGAQIGVYTVEEALGNTTMEGFAYNVFTAWELGSAD
                     QDNGVLLLLAPYEADGGDYYIMRGTGLESRLSVSTLGSLLDEYLEPYWAKGDYDTGTQ
                     KTVRALADRLCSIYGVTLSSDTGSTTYRTGPNIMSMILVILAVILILWIVSVLLRPRR
                     PRPPRGPGYGPGPGGNAAFWYGVGRASRRPRRPPPPPPPRGGGFGGPGPGGPRPGGGP
                     RPGGGPRPGSRPSGGFHSGGGFSSGGGVGRSRPSGGSRPSGGGFRSGGGSTRGGGVGR
                     HH"
     gene            4519..5286
                     /locus_tag="ACZPAX_03485"
     CDS             4519..5286
                     /locus_tag="ACZPAX_03485"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004056784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR1112993.1"
                     /translation="MPKFAVITGASSGIGAEFARQLSARGYNLLLVARRADRLEKLSD
                     QLTMVCEIMTADLSKKSECLRLVKALDDRRVDLFINNAGFGDCGPFLQTDLDKDLDML
                     AVNIRAVHILTKKIGQKLYKQGFGALLNVGSCAGLMPAGPYMATYYATKSYVVSLTSA
                     LAQELREAHSPVYAGCLCPGPVDTEFNAVANVEFALRGITPEYCVRCALDGIARRKTV
                     IVPSPLVAAGMTLGRFLPRPVYIKIAAHQQRKKLYKR"
     gene            complement(5387..6406)
                     /gene="aroF"
                     /locus_tag="ACZPAX_03490"
     CDS             complement(5387..6406)
                     /gene="aroF"
                     /locus_tag="ACZPAX_03490"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005922307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HIR1112994.1"
                     /translation="MIIVLKQHAPAEKVEAFCKELNGMGYEINDSVGSDTHILGLIGD
                     TKALAESWVLANPVVETCRRVSEPYKKANRKFHPDDTIVDVGGHKMGGGYFAVMSGPC
                     SVESKEQITYVAQRVQAAGASILRGGAFKPRTSPYSFQGLRAEGLELLQEARKVTGQP
                     IVTELMNNEHIPLFLDAKVDMIQIGARNMQNFELLKAVGKLNVPVLLKRGLSSTLEEL
                     VMSAEYIMAEGNPNVVLCERGIRTFETSMRNTLDISAVPMLKKMTHLPVVIDPSHAAG
                     IAFMVPDLAKAAVAVGADGLMIETHNDPAKAKSDGAQSLTPDQFDDLMSIIKPELEFF
                     GKKLN"
     gene            6701..7195
                     /gene="ybaK"
                     /locus_tag="ACZPAX_03495"
     CDS             6701..7195
                     /gene="ybaK"
                     /locus_tag="ACZPAX_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007045725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA deacylase
                     activity [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HIR1112995.1"
                     /translation="MPKEAKTNAMRMLERAKVAYSAHEYPHEEGQAVDGANVARLTGQ
                     DPAKVFKTLVTQGADRNYYVFVVPVLAELDLKKAAKAAGVKSVAMIHVADINKVTGYI
                     RGGCSPVGMKKQFVTVFDESCLAQDTMLVSGGRIGTQIECSPADLIKVTRGKTAAITQ
                     EHDA"
     gene            7195..>7816
                     /locus_tag="ACZPAX_03500"
     CDS             7195..>7816
                     /locus_tag="ACZPAX_03500"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005922291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HIR1112996.1"
                     /translation="MRLDKYLAERTGMTRSESRKAVTKGRVMIDGAVCRKADTQLDEH
                     TAAVALDGVPLAGAYRKYVYIMLNKPEGVVSASRDKKDTTVVDLVAADFPRRELFPAG
                     RLDKTSTGFVLLTDDGALAHDILSPAHHVKKQYVVTLDTPLTDAMRRGFAEGVTLADG
                     ETMAPADVEPLTDDGLTVRVTLRQGVYHQIKRMFGVYAAGVNALHRE"
BASE COUNT         1635 a         2377 c         2405 g         1399 t
ORIGIN      
        1 gcacggcgct cgcgccaatg cgctgatttt aggcagcttc cccagcccca aaagccggga
       61 gcagggcttt ttctacggcc acaagcagaa ccgcttctgg ccgatgatcg ccgccgtgac
      121 cggggagcct gtacccgatt gggccgacat cgaggcgaag aaagctatta ttttgaatca
      181 cggcctggcc gtgtgggata ccatcggtgc ctgcgatatc aaaggcgcgt cggatgcgtc
      241 catccgcaac gcggttccca acgatgtagc tgcgctgata cgcaagctgg gcgtgcaggc
      301 ggtgttctgc aacggcgcgg cgtcggggcg catctatgca aaatatgccg agccgctgac
      361 ccacctgtcc gctaccgtgc tgcccagcac cagccccgcc aatgccgcat ggtcccccgc
      421 ccgcctgcaa gccacctggg gcgcagcatt ggaaccattt ttgcagctaa ccggttgcaa
      481 gtgaacggat tttgttctat aataggtata tattatattg gagggactgt tgcatgacca
      541 actggctgga aaaaaccaaa cgcccggtgc tgcccatcta tctggcggcg ctggtctggc
      601 ccattatggc gctggtgctg cccatctaca cgctgggcgg gctgatcgcc acggcggtca
      661 tcagcgcggg cgtgtacatc gcgggcacaa aactctgccc cgtgcgcgtc gtgcgggtgc
      721 cggtcgctta cgccaccggc agtgaggacg tggacgcctt gctcaacggc gtcaccgcca
      781 acctggacgc cctgcacaaa ctgaacgacg aaattcccga caagcagctt tccgacgcga
      841 tggaccgcat ggaaaaagcg ggccgcagta ttgcggacac cgtggcaagg accccggaca
      901 aagcgcgcaa tatcgatcgt tttgcccgct actacctgcc ggagatggtc aagctgatgg
      961 gcgcttacgc cgagctggaa aagcagggtg tcaagggcga gaacgctgac cagatcgaag
     1021 ctgacctgcg ccgcaacgcc gataccaccg ccgccgcctt tgaaaacctg ctggacgcgc
     1081 tgtacagcgc cgaggcaatg gatatttcga ccgacatcga ggtactggac agcatcctga
     1141 aaagccagaa ccttacgaaa taaaagtata tgcagaaagg acaaacgacc atggctgacc
     1201 tgaacaatga actggatctg aacgcccctg ttgcccctac cctgaccctg gactccgccc
     1261 ccgccgcccc gacgctgacc cttgaccccg ccgctgatga aaaaatcgtc gaggaaagca
     1321 agaaggcaac tcccgtgcag gtggaggata cccccctttc ccctgaggag cagaagatgg
     1381 tcaacgattt tgccgaaaaa atcgacctga ctaactccca gatggtcttg cagtacggtg
     1441 ccgccagcca gaagaagctg agcgacttct ccgagacggc gctctcccgc gtcaagaccc
     1501 aggatatggg cgagaccggc gagcttatca ccggcctgat tgccgaactg cagggctttg
     1561 acgccaacgc cgagcagccc aagggtattc tgggcttctt caaaaaggcg tccaacagca
     1621 tcgagcagct caaaactcgc tatgacagcg ccgacaaaaa cgtcgagcgc atcaaggcga
     1681 agctggaaga tcaccaggtc gtcctgatga aggacattgc catgctcgat aagatgtacg
     1741 agtacaacct cgtctacttc aaagagctga cgatgtacat tctggccggc aaaaagaagc
     1801 tggccgatgt gcgtgccaac gagctgaaag ccgcccagga gaaggcccag cgtacccagc
     1861 tgcctgagga tgcccaggcc gcccgcgacc tggccgatat gtgcgatcgc tttgagaaaa
     1921 agctctacga tctggagctg acccgcaacg tctccatcca gatgggcccg cagatccgcc
     1981 tgatccagtc caacgacacg atgatggccg agaagattca gaccaccatc gtgaacacca
     2041 tccccctgtg gaagaaccag atggtgctgg ccctgggcat cgcccacagc cagcaggcta
     2101 tggccgccga gcgcgctgtg accgacgcta cgaacgagct gctgaagaag aatgctgcca
     2161 cgctgaaaca gggcaccatc gatatcgcca aggagtccga gcgcggcatc gtggatatcg
     2221 agaccctgca gcagacgaac aagcagttga tcgagacgct ggatgagctg aacaagatcc
     2281 gcgccgacgg taaggtcaag cgcgccaacg ccgagcagga gcttggccgc attgagggtg
     2341 aactgcgcca gaagatgctg gagatcaacg gctgagaagc tgcgttgtag ggggcggcgt
     2401 cccagacgcc ccgcaaattg gcacacgatt tccgggtggt tgtagggcgg gccagccctc
     2461 tgaccgccgg ggaggggtca agacccctcc ctatatgcac agccatcccg gggcagaata
     2521 tgtaggggcg aacatggttc gcccgcgccg agaatggcag acgaccgttt tccataaccg
     2581 tgcgggccgg gcatgccggc ccctacggtg caatacaggt atttgcaatg caacttgaac
     2641 aacaggaaac ctttaccatc catggcccgc agctggaagt gctgggcgag gtcgagtcaa
     2701 aactgcccgc cccggccaaa aagcgcagca cggcatcggt cgtgctcgtc gcgctggtgc
     2761 tcgtgtcggt gtttggcatc ggcggggcaa agctgaaagg aaaatacaac gatacagcga
     2821 agctctacac cgccatcgac aagtacaaca acagcatcca gaccgatttt ggcacccagg
     2881 tggacgcggc ggctaacctc atcaagcagg tcggctacct ggaaaacgcc ccgacagagg
     2941 ccctgcagga cgcccgggtc gcgctggaag tctggaacaa cacggcagct gaccccgccg
     3001 cccagtacaa cgccaaccgc gatttgtata acgcagtgga tgcgctgtac gcggcggtca
     3061 ataataaagt ggacgccagc acccagcagg ccatcaacaa ccagtacaac gcctttgtct
     3121 cggcccaggc caccattgaa cgcgcggctg cggcctacaa ccagtcggtg gagagctacg
     3181 agacgacgac cgctcagttc cccgccaatg tgatttccgg tatatggggc gtgcgggaaa
     3241 tttcccaatt tgcaccccaa taaaccacga taacaaggga aaattatgaa actgaaaaca
     3301 tttgccaaac gcgcggcggc tgccggtgcg gtggcggcgg cgctggtgct gaccgcactg
     3361 cccgccctgg ctgcgcccgc cgtggatgtg gatgccaaaa acccggtggc cgaccacgca
     3421 aatattctct cggcggatac cgaggaatac atctacgcta tttccagcca gctgtccgat
     3481 gcctgcggtg cgcagatcgg cgtctacacg gtggaggaag cactgggcaa cacgacgatg
     3541 gaaggctttg cctacaacgt ctttacggcc tgggagcttg gctcggctga ccaggacaac
     3601 ggtgtgctgc tgctgctggc cccttatgag gcggacggcg gggactacta catcatgcgc
     3661 ggcaccgggt tggaaagccg cctgagtgtc agcacgctgg gttcgctgct ggatgaatat
     3721 ctggaaccct actgggcaaa aggcgattat gacacaggta cgcagaaaac cgtgcgggcg
     3781 ctggccgata ggctgtgcag catctatggc gtgacgctga gcagcgatac cggcagcacg
     3841 acgtaccgga caggaccgaa cattatgagc atgattcttg tgattcttgc cgttatcctg
     3901 attctgtgga tcgtctcggt gctgctgcgc ccgcgccgtc cacgcccgcc gcgcggcccc
     3961 ggctatggcc ccggccccgg cggcaatgcg gcattctggt acggcgtggg ccgtgcctcc
     4021 cggcgtccgc gccgcccgcc gccccctccc ccgccgcgcg gtggtggatt tggcggcccc
     4081 ggcccgggcg gtccgcgtcc cggcggcgga cctcgtcccg gtggcggccc caggcccggc
     4141 agccgtcctt cgggtgggtt ccactcgggc ggcggtttca gcagcggcgg cggtgtaggc
     4201 cgcagtcgtc cctccggcgg ctcgcgcccc tcgggcggcg ggttccgttc cggcggcggt
     4261 tccacccgcg gcggcggcgt gggacggcac cactaatgta aaaagccttt ccccgagggg
     4321 ctgcgcccgc aggcgcgtgt cggagcgcaa ccgccgtagg cggctcttag cgcgtagact
     4381 gaaggtggcc tgcagggccg gatgaggggc ggcttccccg caaacgaggt gtaacggcga
     4441 ggttcgccct catcagtcgc cttcggcgac agcttccccc tcgggggaag ccttttttgt
     4501 aaatgaagga ggtttcctat gcccaaattt gcagtcatca ccggggcaag ctccggcatt
     4561 ggggccgagt ttgcgcgcca gctttcggca cgcgggtata acctgctgct ggtggcccgc
     4621 cgcgcggacc ggctggaaaa actgtccgac cagttgacga tggtctgcga gatcatgacc
     4681 gctgacctct cgaagaaaag cgagtgcctg cgcctggtca aggcgctgga cgaccgccgc
     4741 gtggatctgt tcatcaacaa cgcgggcttt ggcgactgcg gaccgttttt gcagaccgat
     4801 ttagacaaag acctagatat gctggcggtc aacatccgcg cggtacacat cctgacgaaa
     4861 aaaatcggcc agaagcttta caaacagggc ttcggtgcgc tgctgaacgt cggctcctgc
     4921 gcggggctga tgcctgccgg gccgtatatg gcgacctact acgccacgaa atcctacgtt
     4981 gtcagcctga ccagcgcctt agcccaggag ttgcgcgagg cccacagccc ggtgtacgcg
     5041 ggctgcctct gccccgggcc ggtggatacc gagttcaacg ccgtggccaa cgtggagttt
     5101 gcgctgcggg gcatcacgcc ggaatactgc gtgcgctgtg cgctggacgg catagcccgc
     5161 cgcaagacgg tcattgtgcc cagcccgctg gtagccgccg gcatgacgct gggccgcttc
     5221 ctgccgcggc cggtgtatat caagatcgcc gcccaccagc agcggaaaaa attgtataag
     5281 cgataaaaaa tcgcggctgc ccgggaaagg acagccgcaa tttttgtttt gttgtagagg
     5341 ccgatgcttg catcagcccg caaaggctcc cccctcgggg gaaggcttag ttcagtttct
     5401 tcccaaagaa ctccagctcc ggtttgatga tgctcatcag gtcgtcgaac tggtcggggg
     5461 tcaggctctg ggcaccgtcg ctcttggcct tggccgggtc gttgtgcgtc tcgatcatca
     5521 gaccatccgc gccgacggcc acggcggcct ttgccagatc gggcaccatg aacgcgatac
     5581 ccgccgcgtg gctcgggtcg atgacgacgg gcagatgggt catctttttc agcataggta
     5641 cggcggaaat gtccagcgta ttgcgcatgc tcgtctcaaa ggtgcggatg ccgcgctcac
     5701 acaaaacaac attggggttg ccctcggcca tgatgtactc ggcgctcatg accagctctt
     5761 ccagcgtcga ggacaggccg cgcttcagga gcaccggcac attcagcttg ccgacggctt
     5821 tgagcagctc aaagttctgc atattgcgtg cgccgatttg gatcatgtcc accttggcgt
     5881 ccaggaacag cggaatatgc tcgttgttca tgagttcggt cacgatgggc tggccggtga
     5941 ccttacgggc ttcctgcaaa agttccagac cctcggcgcg cagaccctgg aagctgtacg
     6001 ggctggtgcg gggtttgaac gcaccaccgc gcaaaatgct ggccccggcg gcctgcacgc
     6061 gctgggccac ataggtgatc tgctctttgc tctcgacgct gcagggaccg ctcatcacgg
     6121 caaagtagcc gccgcccatt ttgtgcccgc ccacatcgac gatcgtgtcg tccggatgga
     6181 acttgcggtt ggcctttttg tacggctcag acacgcggcg gcaggtctcg acgaccgggt
     6241 ttgccagcac ccagctttca gcaagggcct tggtgtcacc aatcaggccc aggatgtggg
     6301 tgtcgctgcc gacggagtcg ttgatttcat agcccatgcc gttcagttct ttgcagaagg
     6361 cttcgacttt ttcggcgggt gcgtgctgtt tcaatacgat gatcatggtg ggttctcctg
     6421 tctttcctat ctattacgaa gatgatttct ttcaatgacg gtattctaac actttaaagc
     6481 gctaaagtca agcccccttg cgaaatttgt ttaggggtta tataatagga acgcaaaggc
     6541 ttcccccttg ggggaagctg ttcgcgaagc ggactgatga ggggcggcct tgccattatt
     6601 gcccaacaga gggctgccag gaacacgcgc ccttcatccg gccctgcggg ccaccttccc
     6661 ccaaggggga aggcaaagag aagataaaga ggttgccaat atgccaaaag aagcaaaaac
     6721 caatgcaatg cggatgctgg aacgggcgaa ggtcgcctac tccgcacatg aataccccca
     6781 tgaggaaggc caggctgtgg acggcgcgaa cgtggcgcgg ctgaccgggc aggacccggc
     6841 caaggtgttc aaaacgctgg tcacccaagg tgcggaccgc aattattacg tctttgtcgt
     6901 gccggtactg gccgagttgg atctgaaaaa ggctgccaag gccgcaggcg tcaagagcgt
     6961 ggcgatgatc cacgtcgccg acatcaacaa agtcaccggc tacatccggg gcggctgcag
     7021 cccggtaggc atgaaaaagc agttcgtcac cgtgtttgac gagagctgcc ttgcccagga
     7081 cacgatgctt gtttccggcg ggcgcatcgg cacgcaaatt gagtgctccc ccgccgacct
     7141 tatcaaggtc acgcgcggca aaacggcggc catcacccag gagcacgacg catgatgcgg
     7201 ctggacaagt acctggccga gcgcaccgga atgactcgca gcgagagccg caaggccgtg
     7261 acgaagggac gcgtgatgat tgacggcgca gtctgccgca aggcggacac ccagctggac
     7321 gagcacaccg ccgccgtggc gctggacggt gtaccgctgg caggcgcgta ccggaaatac
     7381 gtctacatca tgctcaataa gcccgagggc gtcgtcagcg ccagccgcga caagaaggat
     7441 acgacggtgg tcgatttggt ggcggcggac ttcccccggc gggagttgtt cccggccggg
     7501 cggctggaca agacgagcac cggctttgtg ctgttgaccg acgacggcgc actggcgcat
     7561 gatatcctct cccctgccca ccatgtgaag aaacagtacg ttgtaacgct ggataccccg
     7621 ctgaccgacg ctatgcggcg cggctttgcc gagggtgtga cgctggccga cggcgagacg
     7681 atggccccgg cagacgtgga accgctgacc gacgacggcc tgaccgtgcg tgtgacgctg
     7741 cggcagggcg tgtaccacca gataaaaaga atgttcggcg tgtatgccgc gggcgtgaac
     7801 gccctgcatc gcgaga
//
LOCUS       DBMDSQ010000060         7790 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_2515_length_7790_cov_2.154376, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000060 DBMDSQ010000000
VERSION     DBMDSQ010000060.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 7790)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7790)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7790
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2515_length_7790_cov_2.154376"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..322)
                     /locus_tag="ACZPAX_03505"
     CDS             complement(<1..322)
                     /locus_tag="ACZPAX_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015563636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III domain-containing
                     protein"
                     /protein_id="HIR1112997.1"
                     /translation="MAATVMELYGSKVFNEHEMRERLPSSTYKSLKATIEKGQPLDLE
                     VANVVASVMKRWAIEQGATHYTHWFQPLTGITSEKHDGFVSPQPDGTAIMEFSGKELI
                     KGEPD"
     gene            641..1162
                     /locus_tag="ACZPAX_03510"
     CDS             641..1162
                     /locus_tag="ACZPAX_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004603861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HIR1112998.1"
                     /translation="MEIERKWMVVGWPEGLEQTEVYKMDQGYISVRPTVRIRREALQG
                     GRTALVLCFKGAGGLSREEIETEINAELFAKLEHLIGKPLIHKERRGYRLPGGLTLEV
                     NCVDPDLPTAFWYAEVEYETEAQALAWDPAACGLADYLHDECTGKPGSSMGEYWLKTR
                     GEEPSPKGEGRNF"
     gene            1263..2756
                     /locus_tag="ACZPAX_03515"
     CDS             1263..2756
                     /locus_tag="ACZPAX_03515"
                     /inference="COORDINATES: protein motif:HMM:NF022702.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3048 domain-containing protein"
                     /protein_id="HIR1112999.1"
                     /translation="MKRLLLVIATLLSLTVLLAACKKSDDTSSAATNPGSTPAQVEAT
                     PAPTELPPYEANVLTGEPKGADYPEGQRITAVMVNNIVAARPQRGLSKADILFEIKVE
                     GGITRFMPVFTDYKTIGEIGPVRSGRDQFFRLILPWQALYIHEGQSVVMQQYGIDYDY
                     GKLNNNDGANGYRDYGRVNWAGKSYNAGTLALEHTMYTNSDNIANYITSQNVDMNRTY
                     NSTFFNFVDYRLGTTRDLSNSLDSAYSDKYGPVVSDGQYIEIVHSQSYKTRFIYDEST
                     NQYKMQQNYSDGQWRDTVDEAADNKVLTFPNVIVLYTDIHTYPGHEKTDLQYVEYAWG
                     GVGYYCYGGKCEKIYWQKGTPLEALRLYYLNEDGTCSDTPLEINTGKSYVAVTDVDFA
                     ENFVHSSLDGVDLSSATTVTYERSYVEDDAKAGETLGSSTDDLTAAATGSGEAETATE
                     APAQETTVNETPAQEEAPVEEAPAEEAPAEETPVENTEAPAEENNVG"
     gene            2825..4264
                     /locus_tag="ACZPAX_03520"
     CDS             2825..4264
                     /locus_tag="ACZPAX_03520"
                     /inference="COORDINATES: protein motif:HMM:NF022702.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3048 C-terminal domain-containing protein"
                     /protein_id="HIR1113000.1"
                     /translation="MRKLQALGGAAALLAALLLGGCSQTAVPATPEQAATAAPEATAE
                     PAPATSTNPLTGLDDGDYTGKRPVAVTLRTLDGAQPQWGVASADVLVEGVTEGSTAGL
                     TAIFADVDSISKVGPVGAGRDLFLQVALPLNAVPVSIDKNIYASNLLNTLGYQDLDGY
                     HIGKAAFAFDQGRQDAGYREENCWYTTGELIRTGLTNYGASAEGDNTPLFRFGTRPEV
                     SPENRNGMNLTVTFSKTDSEQLNYNTGTGLYEKLNADGSPMTDADNGQQVGFTNVFVL
                     YASSGIKDDGYTRQYDMTGGTGLYLQGGAWEQINWTKEDATGPFTLTDADGAPLTVAP
                     GKSFIAIWGGYYGQGLTLTAADGSEQTLPEKPVLLESGVSDEAAAAAEEELSAAQRII
                     DAQAAADQAKADLADALDELQEAQAACDADSENADLLAARDAVQAKIDELNQTIADNQ
                     AILDAAAPEETPAPEEEQPAESESENPAE"
     gene            complement(4417..5052)
                     /locus_tag="ACZPAX_03525"
     CDS             complement(4417..5052)
                     /locus_tag="ACZPAX_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005925467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HIR1113001.1"
                     /translation="MKYYKLHLIRHGLTAGNLQGLYIGSGSDLPLCDEGRAQLKTLVK
                     DFDYPQVPLVFTSPMKRATETAEILFPGVRQIELDDLREMAFGKFEGRAVPELVKDPE
                     FAQWMDPTSRTVPEGAEDRQMFFNRTSSMLMKMFEYMLRTHTEEAACVTHGGVIMNML
                     SQHALPFRKPEEWMTDPGAGYSVRLDAEMWMRDHLAEAYDVVPHGYLDGME"
     gene            5392..6090
                     /locus_tag="ACZPAX_03530"
     CDS             5392..6090
                     /locus_tag="ACZPAX_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007048246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1113002.1"
                     /translation="MGIEFNRIITLLRKERGITQKQAAQDLGISQAQLSHYEKGIREC
                     GLAFVVQVADYYDVSCDYLLGRSAERSGQTINVDDLPEGNTATGGSVYRGSVLPTMYK
                     KLIENSLDILYDKLQESKDKQLVVGVSNYLMLAVYKMFRQMYEASPKNISGMFRVGSA
                     RWNSSADAAMHMAEGDLAATLTGEDGTKDSLEPATLALSTDQLARDYPRHATSLFNLV
                     KNAEESMRGLKKGE"
     gene            complement(6209..6457)
                     /locus_tag="ACZPAX_03535"
     CDS             complement(6209..6457)
                     /locus_tag="ACZPAX_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012705051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HIR1113003.1"
                     /translation="MISTGIVRSIDNLGRVVLPKELRNTYSITPDTPLEIFTDGESIV
                     LRKYRAQGACVFCGEISPDAVLLHGKYICPACRKALGK"
     gene            complement(6757..7167)
                     /locus_tag="ACZPAX_03540"
     CDS             complement(6757..7167)
                     /locus_tag="ACZPAX_03540"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008424637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HIR1113004.1"
                     /translation="MVRPLMTDTVFLAQKAQPADAGDVAVADDLLDTLKAHEDGCVGM
                     AANMIGVNKAIIVFDNDGTPLEMFNPVILYRKGLYLAEEGCLSLQGLRKTKRHRTIKV
                     QWQDRTMQTHVKNFTGWTAQIIQHEVDHCNGILV"
     gene            complement(7232..7432)
                     /locus_tag="ACZPAX_03545"
     CDS             complement(7232..7432)
                     /locus_tag="ACZPAX_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007048365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113005.1"
                     /translation="MLPCQKGCHAYCPGCHKSCPHFRAMQEAQRRDLARKKEYLRRAD
                     ESCRTVLHQCRAAGGYLGPLPY"
BASE COUNT         1826 a         2304 c         2200 g         1460 t
ORIGIN      
        1 catccggctc gcccttgatg agctccttac cggagaactc catgatggcg gttccatccg
       61 gctgggggct gacaaagccg tcgtgcttct cactggtgat gccggtcaac ggctggaacc
      121 agtgggtgta atgggtggcg ccctgctcga tcgcccagcg cttcatcacg ctagccacga
      181 cgttggcgac ctccaggtcc aggggctggc ccttctcaat cgtcgccttc aggcttttgt
      241 aggtagagct gggcagacgc tcacgcatct catgctcgtt gaaaactttg ctgccgtaaa
      301 gttccataac ggttgctgcc atacttctta ctcctttatt tcctctattt acagaaaaag
      361 ggcgctgcga aaacacattc gcagcgccct tgctgttatg cctctattat acggggtaag
      421 tccgcaaaat gcaagtggca atttgacgaa agcagggcgg gattttcagt ggaaaactgg
      481 caggattgtg aggtgcgtgc ggcggacgga atgtggtata atttcagata agggataaaa
      541 gatagcaggg gagggattta tccctcccgt ggaccttatg gcagcgggaa ctttgcgggc
      601 ggatatggaa tccgccccta caggggaggc aaggataaaa atggaaatcg aacgcaaatg
      661 gatggtcgtc ggctggccgg aagggctgga acagaccgaa gtgtataaaa tggatcaagg
      721 ctacatcagc gtgcggccca cagtgcgcat ccggcgcgag gctttgcagg gcgggcgcac
      781 ggcgctggtg ctctgcttta aaggtgcggg cggtttgagc cgggaggaaa tcgaaacgga
      841 aatcaatgcc gaactgttcg ccaagctcga acatttaata ggtaagccgc tcatccacaa
      901 ggagcgccgc ggctatcggc tgcccggcgg gctgacgctg gaagtcaact gcgtggaccc
      961 cgacctgccc accgccttct ggtatgccga ggtcgagtac gaaaccgaag cccaggccct
     1021 cgcctgggac ccggctgcct gcggcctggc cgactacctc cacgatgaat gcacaggcaa
     1081 acccggcagc agcatgggag aatactggct caagacgcgg ggagaagagc cttcccccaa
     1141 aggggaaggc aggaattttt aatttgtaaa atctcctcgc ctacctttac aaacgccccc
     1201 aaaacggata caataagaaa taactactaa accgatacgg aaacccgaaa aggagctatg
     1261 gaatgaaacg tcttctgctt gtgatcgcga cgctgctctc cctgactgtg ctgctggcag
     1321 cctgcaaaaa gagcgacgat acctcctctg cagccaccaa cccgggcagc acccctgccc
     1381 aggtcgaggc gacccccgcg cccaccgaac tgccccccta tgaggccaac gtcctgaccg
     1441 gcgagccgaa gggggccgat taccccgaag gccagcgcat cacggcggtc atggtcaaca
     1501 acatcgtggc ggcccgcccc cagcgcggcc tgtccaaagc cgacatcctg tttgaaatca
     1561 aggtcgaggg cggcatcacc cgctttatgc cggtctttac cgattataag accatcggcg
     1621 aaattggccc ggtccgttcc ggccgtgacc agttcttccg tctcatcctg ccttggcagg
     1681 cactgtatat ccatgaaggc cagtccgtcg tcatgcagca gtacggcatc gactacgatt
     1741 acggcaagct gaacaacaac gacggtgcca acggctaccg cgactatggc cgcgtgaact
     1801 gggccggcaa gagctacaac gccggcacgc tggcgctgga gcacacgatg tacacgaact
     1861 ccgacaacat cgcgaattat atcaccagcc agaacgtgga tatgaaccgc acctacaatt
     1921 ccaccttctt caactttgtg gactaccgcc tgggcaccac ccgcgacttg tccaacagcc
     1981 tggacagcgc ttacagcgat aagtacggcc cggtcgtctc cgacggtcag tatatcgaga
     2041 tcgtccacag ccagtcctac aagacccgct tcatctacga cgagagcacc aaccagtaca
     2101 agatgcagca gaactactcc gacggtcagt ggcgcgatac cgtcgacgag gccgccgaca
     2161 acaaggtgct gaccttcccc aacgtcatcg tcctgtacac cgacatccac acctaccccg
     2221 gccacgagaa aacggacctg cagtacgttg agtacgcatg gggcggcgtc ggctactact
     2281 gctacggcgg caagtgcgag aagatctact ggcagaaggg caccccgctg gaggccctgc
     2341 gcctgtacta cctgaacgag gacggcacct gcagcgatac cccgctggag atcaacaccg
     2401 gcaagagcta tgtggccgtg accgacgttg actttgccga gaactttgtc cacagcagcc
     2461 tggacggtgt tgacctgtcc agcgctacca ccgtcactta cgagcgcagc tacgtcgagg
     2521 acgacgccaa ggccggtgag acgctgggct cttccaccga cgacctgacc gcagccgcca
     2581 ccggcagcgg cgaggctgag accgccaccg aggcacccgc ccaggagacc accgtgaacg
     2641 agacccctgc ccaggaggaa gcccctgtgg aagaggcacc cgccgaagag gccccggctg
     2701 aggaaactcc cgtggagaac actgaggccc ccgcagagga aaacaacgta ggctaaacag
     2761 gtctaacccg acaacgtggc tcgcccggcg gcgggccgcg ttgtttttgt gaaagaggtt
     2821 ttgtatgaga aaattgcagg cattgggcgg cgcggcggct ttgctggccg cactgttgct
     2881 gggcggctgc agccagacgg ctgtgcctgc cacgcctgaa caggcggcca ccgccgcccc
     2941 cgaagccacg gcggaacccg ccccggccac tagcaccaac ccgctgaccg gcttggacga
     3001 cggcgattac accggcaagc gcccggtggc cgtgacgctg cgcacgctgg acggcgcaca
     3061 gcctcagtgg ggcgttgcct ccgcggacgt gctggtcgag ggcgtgactg agggcagcac
     3121 cgcaggactg accgcaatct ttgccgatgt ggacagcatc agcaaagtcg gcccggtcgg
     3181 ggcggggcgc gacctgtttt tgcaggtggc cctgccgctg aacgctgtgc cggtgtccat
     3241 cgacaagaac atttacgcct ccaacctgct gaacacgctg ggctatcagg acctggatgg
     3301 ctaccacatc ggcaaggcag ccttcgcctt tgaccagggc cgccaggacg caggctaccg
     3361 cgaggaaaac tgctggtata ccaccggtga gttgatccgc accggcctga cgaactacgg
     3421 cgcatcggct gagggcgaca acacgccgct gttccgcttt ggcacccggc ccgaagtcag
     3481 ccccgagaac cgcaacggca tgaacctgac cgttaccttc tccaagaccg acagcgaaca
     3541 gctgaactac aacaccggca ccggcctgta tgagaagctg aacgcagacg gcagcccgat
     3601 gaccgacgcc gacaacggcc aacaggtggg ctttaccaac gtgttcgtgc tctacgcgtc
     3661 cagcggcatc aaggacgatg gctacacccg ccagtacgat atgaccggcg gcaccggcct
     3721 gtacttgcag ggcggcgcgt gggagcagat caactggacg aaagaggatg ccaccgggcc
     3781 gttcactctg acggatgccg acggtgcgcc gctgactgtg gcccccggca agagctttat
     3841 cgctatctgg ggcggctact acggccaggg cctgaccctg accgccgccg acggcagcga
     3901 gcagactctg ccggaaaagc ccgttctgct ggaaagcggt gtctccgatg aggccgccgc
     3961 agccgccgag gaagaactga gcgcggcaca gcggatcatc gacgcacagg ctgccgcgga
     4021 tcaggccaag gctgatctgg ccgacgcgct ggacgaattg caggaggctc aggctgcctg
     4081 cgatgccgac agtgagaatg ccgacctgtt ggctgcccgc gacgctgttc aggccaaaat
     4141 cgatgagctg aatcagacca tcgctgacaa tcaggctatc ctagacgccg ccgcgccgga
     4201 ggaaaccccg gcccccgagg aagaacaacc ggcagaatcc gagtccgaga acccggcaga
     4261 ataaaaacga cttgcagggg cggacgcctg catccgcccg cggggcgtcg aggacgccgc
     4321 cccctgcaat aaaaaagcac ctgcttacca taagcaggtg catttttgtt tggaatttct
     4381 gcttccttct ttgtaaaaaa ggaacaaaac cccgcattat tccatcccgt ccagataccc
     4441 gtgggggacg acatcgtacg cctcagccag atgatcacgc atccacattt cggcgtccag
     4501 ccgcacactg taccccgcgc ccgggtcggt catccactcc tccggcttgc ggaaaggcag
     4561 cgcgtgctgg gacagcatat tcatgataac gccgccgtgg gtcacgcagg cggcctcctc
     4621 ggtatgggtg cgcagcatat actcaaacat cttcatcagc atcgaggacg tgcggttgaa
     4681 aaacatctgc cggtcctcgg caccttcagg caccgtgcgg ctcgtcgggt ccatccactg
     4741 ggcaaactcc gggtccttta ccaactccgg cacggcgcgg ccctcaaact tgccaaaggc
     4801 catctcgcgc aggtcgtcca gttcgatctg ccgcacgccc gggaacaaaa tctccgccgt
     4861 ctcggtggcg cgcttcatcg ggctggtgaa caccagcggc acctgggggt aatcaaaatc
     4921 cttcaccagc gttttcagct gggcgcggcc ctcatcgcac agcggcaggt cactgccact
     4981 gccaatgtac agcccctgta aattgcccgc cgtcaggccg tggcggatca aatgcagttt
     5041 gtaatatttc actatgcagc tctcactttc gtaaatgtct acctatcagc ataccctaaa
     5101 atcgtgtcct gcgcaaccat aaattgttac aatttggtta cagccgacgc cggctgcgcc
     5161 caactgccaa aatttgccca caatatttat agctgtactc gctgtataat gactagatgt
     5221 gggaaaagtc ctgcacttcg gcgctttgac ggctttttca gcggaaaatc tgtcgaattt
     5281 tcgggtttac aaagcgtttt ccacccgcta taatatacag cgtgtagaaa acatagcagg
     5341 ggcatactgc ggcccggcgc aagccggcaa tgccccggaa caggatagat catggggatc
     5401 gaatttaatc ggatcatcac gctgctgcgc aaagagcgcg gcatcaccca aaagcaggcg
     5461 gcgcaggacc ttggcatcag ccaggctcag ctttcccatt acgaaaaagg catccgcgag
     5521 tgcggcctgg ccttcgtggt gcaggtggcg gactactacg acgtcagctg cgactacctg
     5581 ctgggccgca gtgccgagcg cagcggccag accatcaacg tggacgacct gcccgagggc
     5641 aacaccgcca ccggcggcag cgtctaccga ggcagtgtcc tgcccacaat gtacaaaaaa
     5701 ttgatcgaaa attcgttgga tatcctctac gataaactgc aggagagcaa ggacaagcag
     5761 ctggtcgtgg gcgtcagcaa ctacctgatg ctggctgtct acaagatgtt ccgccagatg
     5821 tacgaggcat cgccgaaaaa tatcagcggt atgttccgcg tcggctcggc ccgctggaac
     5881 agcagcgccg acgccgccat gcacatggca gagggcgacc tggcggccac cctgaccggc
     5941 gaggacggca cgaaggacag cttggaacct gccaccctgg ccctgagcac cgaccagctg
     6001 gcccgggact acccgcgcca cgccaccagc ctgttcaacc tcgtcaaaaa cgccgaggaa
     6061 tccatgcgtg ggctgaaaaa gggagaatga agccgttgcc ccatagaaaa tgtagggggc
     6121 aaacatggct cgcccgccaa caaaacggac acaaaaaaat aagcagagca tccaacgcgg
     6181 atgccctgct ttacttttta tcgatgcctt atttccccag tgccttgcgg caggccgggc
     6241 aaatatactt cccatgcagc aaaactgcgt cgggcgaaat ctcgccgcag aatacacagg
     6301 cgccttgggc acggtacttg cgcagcacaa tggattcccc atcggtgaaa atctccagcg
     6361 gggtgtcggg cgtgatgctg taggtgttgc gcagctcctt gggtaatacc acgcgcccca
     6421 ggttgtcaat actgcggacg atgccggtag aaatcatctt tctaacctcc tattttacgt
     6481 ttcgacataa caaatgccga aaaacggtgt ccgttcctgt cgtacagtcg gacttgttgt
     6541 ctctattata cgagctattg gaaacattgt caatatttgg aagaaaacaa aaatgaggat
     6601 atttcgtgaa tttttgttat ccacacgaat aggtaatagt gtttgttgcg cagtcaatac
     6661 gttttgtaac aaatttgaca cagacaggat atttgttaaa ttagatagga gataacaaaa
     6721 ggcgatgcgc tccgaaaagc gcatcgcctt accttattat acaagaatgc cgttgcagtg
     6781 gtcgacctcg tgctggatga tttgcgccgt ccagccggta aaattcttta cgtgggtctg
     6841 catggtgcga tcctgccact gcactttgat ggtgcggtgg cgcttggtct tgcgcagccc
     6901 ttgcagggac aggcagcctt cctcggccag gtacagcccc ttgcggtaga ggatgaccgg
     6961 gttgaacatt tcaaggggag tgccgtcgtt gtcgaacacg atgatggctt tgttcacgcc
     7021 aatcatgttg gcggccatgc cgacacagcc gtcctcgtgg gctttcagcg tgtcgagcaa
     7081 atcatcggca acagcaacat ccccggcgtc agcgggctgg gccttctgcg ccaggaaaac
     7141 ggtatcggtc ataagcggtc gaaccatggg ggaaacctcc tgcgtgtact attttatagt
     7201 agtataccgc aggcagagta cgctgtaaag ttcaataggg cagcgggccc agatacccgc
     7261 ccgccgcgcg gcattggtgc agcacggtgc ggcaggattc atcagcgcgg cgtaagtact
     7321 ccttcttgcg ggccaggtcg cggcgctggg cctcctgcat cgcgcggaaa tgcgggcagg
     7381 acttatggca accggggcaa taggcatgac agcctttttg acaaggcagc atagagaaca
     7441 cgctcctttc ggcagatcag gcaacagaat gggaataact ggtacaagat atagtaggaa
     7501 agaaccgttc ttatgcctat agtatacacc ataagatttg tcgcatcaag gggaaatgtg
     7561 taagtgtttt gtaaagaatt gtgcgtgacc acacagcctt caaaaaaatt aaaaaacttt
     7621 taaaaaccct attgacattt ttggttggac aggatataat atacaagctg tcacggagac
     7681 gcgatagcac agcaggacct tgaaaattga acaaaactga aacttgtgga accttgaacg
     7741 tgggtttgga aacccacgat aatcaattcc aaaatttaca agtaattcat
//
LOCUS       DBMDSQ010000061         7755 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_2528_length_7755_cov_1.826496, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000061 DBMDSQ010000000
VERSION     DBMDSQ010000061.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 7755)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7755)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7755
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2528_length_7755_cov_1.826496"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..2445
                     /locus_tag="ACZPAX_03550"
     CDS             <1..2445
                     /locus_tag="ACZPAX_03550"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HIR1113006.1"
                     /translation="IANIIRFGSAHGSRELPRTVTEADLNDLRTAVANGRDLPRETAY
                     LAVRPARPWNVLAITFTNKAAGELKERLRAMLGDTLGGDVNASTFHSACVRMLRRDAE
                     RIGFPKSFTIYDSDDQQRVIKQIYKDLMIDDKFLPVKSAIGQISSYKDKLMSAEEVAA
                     EIPANTKAQLISKIYTTYANRLKTAGAMDFDDLIFHTVKLLKNDAEAREYYQNRFRYV
                     VVDEYQDTSVAQFHLVRLLAGGTNNVCVVGDDDQSIYKFRGATIENILNFEKVFAGAK
                     TIRLEQNYRSTANILNAANSVIKNNMGRKGKTLWTESGDGEKIHHYTAINEQDEASHI
                     ADIIGEHLREGASLKDHAVLYRMNAQSNPIETYFARAGIPYRIVGGQRFFDRKEVKDI
                     NSYLAVIVNPRDDVRLRRIINEPARKIGATTIEKIGELAAGKGVPMMEVIAHVREYPE
                     LQRATAALERFYEMYRELCDLSVSEPLDVFVGDVIAKTGYEAMLKAMKEEGETRRENL
                     GQLVSSIKTYADQNGEDATLSGFLEEVALISDLDSYDDDADSVTMMTIHSAKGLEFPY
                     VFVVGMEDGIFPGDMAKYNEEDMEEERRLCYVAITRAKKELYLSTSRTRMIFGQTRRN
                     PPSTFLSEIDPDLLDESQSPELAGYGGGFGAGYGTYSTNVPGGRSGYSGASRGYLNSE
                     YNAHPGGFGGGYSSGFVSGGHESPNSYGGRHQVQSTGFGSGYGRSRAGGNTAPAGAGT
                     STLAGAPGAAPKKPEVKKTVSYAPGDIVDHRVFGRGKVLKATPIAGDCIVEIQFDRVG
                     VKKTMANYAPLKKVEE"
     gene            2447..3220
                     /gene="thyX"
                     /locus_tag="ACZPAX_03555"
     CDS             2447..3220
                     /gene="thyX"
                     /locus_tag="ACZPAX_03555"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HIR1113007.1"
                     /translation="MLVQLIAHTNDPEKTIAAAAKLCYSDAHIETLLDGLTPEKTEAF
                     LQRLNDVGHASPIEHASFTFGIEGVSRTFLAQVTRHRIGSFSVQSQRYVRLEDFRYVI
                     PPEIEAIPEARAQFIASMNDDAKKYLELVQTLENAHTARLMAEGLNEKAARAKASKQA
                     NEDARFVLPNACETKMVMTMNCRSLQNFFNLRCCNRAQWEIRAVADEMLRLVMPIAPH
                     IFASAGPRCLVGPCPEGRMCCGQQAEVRAKYAKLKEEAV"
     gene            3221..>3416
                     /locus_tag="ACZPAX_03560"
     CDS             3221..>3416
                     /locus_tag="ACZPAX_03560"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - dTMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HIR1113008.1"
                     /translation="MGQLIIFEGLDGSGKGTQTKLTAQRLQEQGKDLRQVTFPNYESD
                     SSALVKMYLSGAFGDKPDDVN"
     assembly_gap    3417..3426
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3427..3843
                     /locus_tag="ACZPAX_03565"
     CDS             <3427..3843
                     /locus_tag="ACZPAX_03565"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - dTMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HIR1113009.1"
                     /translation="EFYREGGLVLSDRYTTSNAVHQCSKLPPMHWDGFLNWLFDFEYK
                     KFGIPAPDAVIYLAVDPDVSQKLITERYHGDETKKDIQEKDTEYMARSRAAAEYCART
                     LGWHRIECTVEANGAKSMRSVEDINAEILAKLNSIL"
     gene            3896..4717
                     /locus_tag="ACZPAX_03570"
     CDS             3896..4717
                     /locus_tag="ACZPAX_03570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113010.1"
                     /translation="MLRKATQDDLPALVALRCKAARTNRTDAAGWLQNVAGLENILLL
                     VKSGAPPAAMLAAVPVEYGQHHGVWLCGLAGEQGVPLDRLLPKLLESCLRAYGASGFD
                     FAVMTPEGAQHADALKQLGFTAQLPLRVLQTPIRRDLLAQASFDSLTVHKLLEMRHIY
                     QPGCITLPESAMNEIMTQLYRRGLTVVSNRRGYGLYYTSGDTMQFLELQADNDHCADR
                     LLQAAREKTGAANARILLAESQTLYLGAGRRCGYGMLAFLGKPFPVTDAYFRMLL"
     gene            4849..6165
                     /gene="mltG"
                     /locus_tag="ACZPAX_03575"
     CDS             4849..6165
                     /gene="mltG"
                     /locus_tag="ACZPAX_03575"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HIR1113011.1"
                     /translation="MRINRSNPDQPAMQQPQAQPQAAPAAPKPAPKREPPKPKKEKPK
                     KKPKHEKYDEYEDDEEEPRKRRFPFGCLIVLILLALVGFGGYKVSQFYAELDGQGTLG
                     AAQTITVPEGSSVASIATQLKDAGVIQYDWLFKQYVKYSGKAGEIQYGDFEVQSGMAY
                     NDIIKALSVVTRRATVNVTIPEGTTAVGVAQIFVDAGLVDDVDTFLSCANGNDGSDWS
                     KYDFWNAIPDNDRLMKCEGYLFPDTYNLYADEDVYYYVDQLYGEFANKTADLTDTIAA
                     KGTSLDDVVKLASFIQEEAGLESEDAKVSACFHNRLESSDPQWAEHKLESNACSYIMQ
                     DSENNYLWNSPTAEYFGWPEDGAIPDDVLALYDTYAIGGLPAGPISCPGYAAIEAALN
                     PDQEYLDEGYFFFVTGHPDTDVAGQYFYAKTADEHYQNCVKAGWAS"
     gene            6305..7531
                     /locus_tag="ACZPAX_03580"
     CDS             6305..7531
                     /locus_tag="ACZPAX_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HIR1113012.1"
                     /translation="MLQQPELLAPAGSLETLKYAVMYGADAVYCALPEFGMRAAPVNL
                     TVGELREGCIFAHARGKKVYLTLNTLPTNEELSKLPQYIQDAAEAGVDAFIIADLGVL
                     ALAKKYAPQVERHVSTQAGITNYEAAKVCYELGAKRVVLARELPLTEIAQIRDNCPAD
                     LELEAFVHGAMCMSVSGRCLLSSYMAGRSGNRGECAQPCRWKYNLVEEHRPGQYMEIG
                     ETPDNGSYILNANDLCTAPFIDLICKAGVDSLKIEGRAKTFYYVASVVSAYRRALDTF
                     LADPYNDNFELPDDVIEELNRTSHRHYSPGFYFGKEQAQQTPSHTYVRDWDFIGTVDS
                     WENGIAHCTQRGKFSVGDSIEILQPDGSVVKLTPAWIENADGDRVDATPHPMMQYTIP
                     CETPLMAYSLIRMQKQ"
     gene            7668..7744
                     /locus_tag="ACZPAX_03585"
     tRNA            7668..7744
                     /locus_tag="ACZPAX_03585"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7702..7704,aa:Asp,seq:gtc)
BASE COUNT         1717 a         2344 c         2239 g         1445 t
ORIGIN      
        1 attgccaaca tcatccgttt tggctcggcc cacggcagcc gtgagctgcc ccgcactgtg
       61 accgaagctg acctcaacga cctgcgcact gccgtcgcca atggccgtga cctgccccgg
      121 gagaccgcct atctggccgt gcgcccggca cgcccctgga acgtgcttgc catcaccttt
      181 acgaacaagg cggcgggaga gctgaaagag cgcctgcgcg ccatgctggg ggacaccctg
      241 ggcggcgacg tcaatgcgtc tacctttcac tctgcttgtg tgcggatgct gcgccgtgac
      301 gccgagcgca tcggctttcc caaaagcttt accatctacg actcggacga ccagcagcgc
      361 gtcatcaagc agatttataa agatttgatg attgacgaca aattcctgcc ggtcaagtcg
      421 gccatcgggc agatttcttc ctataaggat aagctcatgt ccgctgagga agtggctgcc
      481 gagatccctg ccaataccaa ggcgcagctg atctccaaaa tctataccac ctacgcaaac
      541 cgtctgaaaa ccgcaggtgc aatggacttt gacgacctga tcttccacac ggtcaagctg
      601 ctgaaaaatg acgcagaagc gcgggaatat taccagaacc gcttccgcta tgtcgtcgtg
      661 gacgagtatc aggacacgag tgtggcgcag ttccatctgg tgcgtctgct ggcgggcggc
      721 acgaacaatg tctgcgtcgt cggcgacgat gaccagtcta tctacaaatt ccgcggtgca
      781 acgattgaaa atattctaaa cttcgagaag gtctttgccg gtgccaagac aattcgactg
      841 gagcaaaact atcgctccac cgctaatatt ctgaatgctg ccaacagcgt cattaagaac
      901 aacatgggcc gcaagggcaa gaccctgtgg accgagagcg gcgacggcga gaaaatccac
      961 cactataccg ccatcaatga gcaggacgag gccagccata tcgcggatat catcggcgag
     1021 catctgcgcg agggtgcttc cctgaaagac cacgccgtgc tctaccgcat gaacgcccag
     1081 tccaacccca tcgagactta cttcgcccgc gcgggcatcc cgtaccgcat cgtcggcggc
     1141 cagcgcttct ttgaccgcaa ggaagtcaag gacatcaaca gctatctggc cgtcatcgtc
     1201 aacccccgcg acgatgtgcg cctgcgccgc atcatcaacg aaccggcacg taaaattggc
     1261 gcaacaacga ttgaaaagat cggtgagctg gctgccggta agggtgtgcc gatgatggag
     1321 gtcatcgccc atgtgcgcga gtatcccgaa ttgcagcggg ccaccgccgc actggaacgc
     1381 ttttacgaga tgtaccgtga gctgtgcgac ctctcggtta gtgagccgct ggatgtgttt
     1441 gttggcgacg tcattgccaa gaccggctac gaggccatgc tgaaagctat gaaggaggag
     1501 ggcgagaccc ggcgcgagaa cctgggtcag ctcgtcagct ccatcaagac ctacgccgat
     1561 cagaacggcg aggacgcgac actctccggc ttcttggagg aagtcgcgct gatctccgac
     1621 ttggacagct atgacgacga cgcggacagc gtcacaatga tgacgataca ctcggccaag
     1681 ggcctggagt tcccctacgt ttttgtcgta ggtatggagg acggcatctt ccccggtgat
     1741 atggccaagt ataatgagga ggacatggag gaggagcgcc gcctttgcta tgtcgccatc
     1801 acccgcgcaa aaaaggaact gtacctgtct acttcccgca cgcggatgat cttcggccag
     1861 acgcggcgca acccgccgtc caccttcctg agcgagatcg acccggactt gctggacgag
     1921 agccaaagcc ccgagcttgc cggctatggc ggcggcttcg gtgcagggta cggcacatac
     1981 agcaccaatg tcccgggcgg gcgcagcggt tactccggtg cgtcgcgcgg gtatctcaac
     2041 agcgagtata acgcccaccc cggtggcttc ggcggcggat acagcagcgg ctttgtcagt
     2101 ggcgggcacg aaagccccaa ctcctacggc ggccgccatc aagtacagtc caccgggttc
     2161 ggctccggct atggccgcag ccgcgcgggc ggcaataccg ccccggccgg tgcaggtaca
     2221 tccacactgg cgggtgcgcc cggtgccgcc cctaaaaagc cggaagtgaa aaaaaccgtc
     2281 agttatgcgc ccggcgatat cgtggaccac cgcgtctttg gccgcggcaa ggtgctgaaa
     2341 gccacaccca ttgcggggga ctgcatcgtt gagattcagt tcgaccgcgt cggcgtcaaa
     2401 aagacgatgg ccaactatgc accgctgaaa aaagtggagg aatgatatgt tagtccagct
     2461 cattgcccat acgaacgacc cggaaaaaac catcgccgcg gcggcgaagc tctgctactc
     2521 tgacgctcac attgaaacgc tgttggacgg tctgacgccc gaaaagaccg aggcattttt
     2581 gcagcgtctc aacgatgtag gccacgccag ccccattgaa cacgccagct tcaccttcgg
     2641 tattgagggt gtttcccgca cgtttcttgc gcaggtcacg cggcaccgca tcggcagttt
     2701 cagcgtgcag agccagcgct atgtgcggct ggaagatttt cgctacgtca tcccgccgga
     2761 gatcgaggcc atccccgagg caagggcaca gtttatcgcc tcgatgaacg acgacgctaa
     2821 aaaatacctg gaactggtcc agacgctgga aaacgcccat accgcccgcc tgatggctga
     2881 gggtctgaac gagaaagccg cccgtgccaa ggcaagcaag caggccaacg aggacgcccg
     2941 ctttgtgttg cccaacgcct gcgagacgaa aatggtcatg acaatgaact gccgcagtct
     3001 gcagaacttc ttcaacctgc gttgttgcaa ccgcgcccag tgggaaattc gcgccgtcgc
     3061 cgatgagatg ctgcggctgg tcatgcccat tgcgccccac atttttgcaa gtgcaggccc
     3121 gcgctgcctg gtcggcccct gcccggaggg ccgtatgtgc tgcggccagc aggccgaggt
     3181 gcgcgccaaa tacgccaaac tcaaagagga ggcagtgtaa atgggacagc ttatcatttt
     3241 tgaggggctg gatggctccg gcaagggtac ccagacaaaa ctgacggccc agcgcttaca
     3301 ggagcaggga aaagacctgc ggcaggtcac cttccccaac tacgagagcg attcctcagc
     3361 actggtcaag atgtacctgt ccggcgcgtt cggcgacaag cccgacgacg tcaatgnnnn
     3421 nnnnnngagt tctaccgcga gggcggcctg gtgctgtccg accggtatac gacttccaac
     3481 gccgtgcatc agtgctccaa gctgccgccg atgcactggg acggattctt gaactggctg
     3541 ttcgactttg aatataaaaa gttcggcatc cccgcgcccg acgctgtgat ttaccttgcc
     3601 gtcgacccgg atgtctccca gaagctcatc accgagcgct accacggcga cgagacgaag
     3661 aaagatatcc aggagaagga caccgagtac atggcccgca gccgcgccgc cgccgagtac
     3721 tgcgcccgca cgctgggctg gcaccgcatt gaatgcaccg tcgaggcaaa cggcgcaaaa
     3781 tccatgcgct ctgtcgagga tatcaacgcc gagatacttg cgaaattgaa cagtatcctg
     3841 taaaatagtg tagatattgc acctgcaaaa cgaaccgaaa aaaagaggtg accccatgct
     3901 gcgcaaagcg acacaagatg atttgcctgc gctggtggcg ctgcgctgca aggctgccag
     3961 gaccaaccgc accgacgccg caggctggct gcaaaatgtg gccgggctgg aaaatatcct
     4021 gctgctggta aaatccggcg caccgcccgc ggctatgctg gcggctgtgc cggtggaata
     4081 cggccagcat cacggcgtct ggctctgcgg cctggcagga gagcagggcg tcccgctcga
     4141 ccgtctgctg cccaaactgc tggaaagctg cctgcgggct tatggggcca gcggctttga
     4201 ctttgccgtt atgacgccgg agggcgcgca gcacgccgac gcactgaagc agctgggatt
     4261 caccgcccag ctgccgctgc gggtattgca aacgcccatc cgccgcgacc tgctggccca
     4321 ggcatctttc gacagcttga ccgtgcataa attgctggaa atgcgccata tctaccagcc
     4381 cggctgtatc accctgccgg agtcggccat gaatgagatc atgacccaac tgtaccggcg
     4441 cgggctgacc gtagtgtcca atcggcgcgg atatgggtta tactatacca gcggggatac
     4501 gatgcaattt ctggaattgc aggccgacaa cgaccactgc gccgaccgcc ttttgcaggc
     4561 cgcgcgggaa aagaccggcg cagccaacgc gcgcatattg ctagctgaaa gccagaccct
     4621 ttacctcggc gcaggccgcc gctgcggtta cggtatgctt gcctttttgg gcaagccgtt
     4681 cccggtcacc gatgcgtatt ttaggatgct gctgtaaagg gaaccgggca cgcccggccc
     4741 gcagttcaac cataaagaac gtttctcgtt tcgttgaggg ccgcacatat gcggccccta
     4801 cccggcgtta aaaaaatcaa ccagaaatca aaaagttagg agtatcccat gaggatcaat
     4861 cgttccaacc cagatcagcc cgcgatgcag cagccgcagg cacagcccca ggctgcgcct
     4921 gcggccccca aaccggcccc caagcgtgag ccgcccaaac cgaaaaagga aaagcccaaa
     4981 aagaaaccca agcacgaaaa atatgacgag tatgaggacg acgaagaaga gccgcgcaag
     5041 cgccgtttcc cgtttggctg tctgattgtg ctcattcttc tggcgctggt gggctttggc
     5101 ggctacaagg tcagccagtt ctatgcggag ctggacggcc agggcacgct gggcgcggcc
     5161 cagaccatca ccgtgccgga aggatcctcc gtcgccagca ttgccaccca gctgaaagat
     5221 gcaggtgtta tccagtatga ctggctgttc aagcagtatg tcaagtacag cggcaaggcg
     5281 ggtgagattc agtacggcga cttcgaggtt cagtccggta tggcctacaa tgatatcatc
     5341 aaggcgttgt ccgtcgtgac ccgccgcgcc accgtcaacg tcacgattcc cgagggtacg
     5401 actgccgtcg gtgtggcaca gatttttgtg gatgcaggcc tggtggacga tgtggatact
     5461 ttcctgtcct gcgccaatgg caacgacggc tcagattgga gcaagtacga tttttggaac
     5521 gccatccccg ataatgaccg cctcatgaag tgcgagggct accttttccc ggacacctac
     5581 aacctctacg ccgacgagga cgtctattat tatgtagacc agctctacgg ggaattcgcc
     5641 aacaagactg ctgacttgac cgacacgatt gccgccaagg gtacgagcct ggacgatgtg
     5701 gtcaagctgg ccagcttcat ccaggaggag gctggtttgg agagcgagga tgccaaggtc
     5761 agcgcctgct tccacaaccg cttggaatcc agcgacccgc agtgggcgga gcataagctg
     5821 gaatccaacg cctgcagcta tatcatgcag gatagcgaga acaactatct ctggaactcc
     5881 ccgacggctg agtatttcgg ctggccggag gatggcgcaa ttcccgacga tgtgctggct
     5941 ctgtacgaca cctacgccat cggcggcctg ccagcgggcc ccatctcctg cccgggttat
     6001 gccgccatcg aggccgcgct gaaccccgac caggagtatc tggacgaggg ctacttcttc
     6061 ttcgtcacag gccaccctga caccgacgtc gcaggccagt atttctacgc caagaccgca
     6121 gacgagcact accagaactg cgtcaaagcg ggctgggcgt cgtaaggcac gaagccgcct
     6181 tgtaggggcc acacatatgc ggccctcaac cttgctttga ccgcctgtct gcctttcacg
     6241 acagggccag gcatgcccgc ccggcccctg cgggacgaat catgtgaaaa cggaggaatc
     6301 atcaatgctg caacagccgg aactgctggc ccctgcgggc agcctggaaa cgctgaaata
     6361 cgccgtcatg tacggcgcgg atgccgtcta ctgcgccctg ccggagtttg gtatgcgcgc
     6421 agccccggtc aacctgacgg tgggagaact gcgcgagggc tgcatctttg cccacgcccg
     6481 cggcaaaaag gtctacctga ccttgaatac cctgcccacc aacgaggaac tgtccaaact
     6541 gccccagtat atccaggatg ctgccgaggc aggcgtagat gcctttatta tcgctgacct
     6601 gggcgtgctg gcattggcta aaaagtatgc cccgcaggtc gagcgccacg tctcgaccca
     6661 ggcgggcatc acaaactacg aggccgccaa ggtctgctat gagctggggg ccaagcgcgt
     6721 tgtgctggcc cgtgagctgc ccctgaccga aattgcccag atccgcgaca actgcccagc
     6781 tgatttggag ctggaagcct ttgtccatgg cgctatgtgc atgagcgtgt cgggccgctg
     6841 cctgctgtcc agctacatgg cgggccgcag tggcaatcgc ggcgagtgcg cccagccctg
     6901 ccgctggaag tataacctcg ttgaggaaca ccgccctggc cagtacatgg agatagggga
     6961 gacgcccgac aacggcagct atattttgaa cgccaacgac ctgtgcacag cgccgtttat
     7021 cgacctgatc tgcaaggcgg gcgtggactc gctgaagatc gaaggccgcg ccaagacgtt
     7081 ctactatgtg gcgtcggtag tcagcgccta ccgccgcgca ctggatacct tccttgctga
     7141 cccctacaac gataattttg aactgcccga tgatgttatc gaggaactga accgcacgag
     7201 ccaccgccac tactcgccgg ggttctactt cggcaaggaa caggcccagc agacccccag
     7261 ccacacctat gtgcgagatt gggattttat cggcacagtg gacagctggg agaacggcat
     7321 tgctcactgt acccagcgcg gcaagttcag cgtgggggac agcatcgaga ttttgcagcc
     7381 cgacggcagt gtcgtcaagc tgacccccgc ctggatcgag aacgccgacg gtgatcgtgt
     7441 ggacgccacc ccgcacccga tgatgcagta caccatcccc tgtgaaactc cgctgatggc
     7501 gtacagcttg atccgtatgc agaagcagtg atgttgtgtg gaacacatca caaaaatatt
     7561 ttaaaattct cgtaaaaatc ccttgacttt ttatggcata tcccttataa tatttcttgc
     7621 gccggtcgtg agggcacaac gctctgcctc ctgcgcaagc tgggtatggc ccggtagttc
     7681 agttggttag aacgctagcc tgtcacgcta gaggtcgtca gttcgagcct gattcgggtc
     7741 gccatatgct gctat
//
LOCUS       DBMDSQ010000062         7636 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_2574_length_7636_cov_1.613006, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000062 DBMDSQ010000000
VERSION     DBMDSQ010000062.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 7636)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7636)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7636
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2574_length_7636_cov_1.613006"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..293
                     /locus_tag="ACZPAX_03590"
     CDS             <1..293
                     /locus_tag="ACZPAX_03590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113013.1"
                     /translation="KPFIYTFAIDHLGLTPCTMAPNLPVSIETPAGIWSPKEAGKVEY
                     DGVLHPLYWGMANSRNNCSAWIMKQAKQPAAVKKLQKNFHKKRVFPAVLSYL"
     gene            323..1324
                     /locus_tag="ACZPAX_03595"
     CDS             323..1324
                     /locus_tag="ACZPAX_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HIR1113014.1"
                     /translation="MTIREETEAIERQTLSRYATLSENSLGRRTPLEDDPIRPCFQRD
                     RDRILHCKAFRRLKQKTQVFLSPEGDHYRTRLTHTLEVSQIARTISRALRLNEDLTEA
                     IALGHDLGHTPFGHAGERALNRLCPGGFTHYRQSLRVVDYLERDGQGLNLTWEVRNGI
                     VTHTSGAWARTPEGCVVRRADRIAFLNHDIEDAITAGVLDARQLPPEATAVLGATKSQ
                     RITTMITDLIAHSGEGQINFSPEVEAAYAILKDFMYSTIYVDKEAKREEKKVDKLISE
                     LYTRLSDEPALMPNFYMQIAYNEGVDRAVTDYISGMSDEFATRLFEELFVPQKWHVL"
     gene            1448..3232
                     /gene="dnaG"
                     /locus_tag="ACZPAX_03600"
     CDS             1448..3232
                     /gene="dnaG"
                     /locus_tag="ACZPAX_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015538448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HIR1113015.1"
                     /translation="MIPQEYIQEVVRRNDIEEIIGQYVQLRRRGRTATGLCPFHNEKT
                     PSFVVYPDTQSFYCFGCGAAGDVISFVRKYNNLGYVEAVKQLASRAGMPLPEEDDRES
                     RARQRLLEINRCAARYFYENLNAKTPEAAMARRYWKEKRGLSDAAIRRFGLGYAPDDF
                     VGLLHYLKHLGFSESELETSGLVKRSAKGNLYDIFRHRVMVPIIDVRGAIIAFGGRVL
                     DDSKPKYINSPETMVYHKSRTLFALNVAKKSKSKRYILCEGYMDVISMHEAGFDTAVC
                     ACGTALTAEQAKLLSEYADEVVLSYDSDEAGQKATERSLGIMANTPMKVSVLSYQGAK
                     DPDEFIKKYGRERFEMLLNGTANPTEFQLKKAKSKYDLRSDDGRLSYIREAIDILTER
                     GVSPTARDVYAGRLADETGVSKQAIVSQMQGAVRTAERRNYRKEQRDLSKEGIAADIR
                     VPYTSGGESALGAASAARQLAAALLQKPEEVPYVRSRLDMEMVLDPEMKTVLNAIFRC
                     VDEQLPLNLTTLQQMLDDKAFGQIALAQAQNHGTPLQRQDIDMYLDRMQTAKPASEKV
                     KQMDADQFANFFADIGKRKGAHQPTPDE"
     gene            3285..4382
                     /gene="rpoD"
                     /locus_tag="ACZPAX_03605"
     CDS             3285..4382
                     /gene="rpoD"
                     /locus_tag="ACZPAX_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015518931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HIR1113016.1"
                     /translation="MAEKKDPIRNLIDSGRRAGKLTSTEISDAMEEAGNVLEVEAMEK
                     LYEELESNGIEVIDDTPVEVDDTVAAPDDGADDYEDGLTADGITIDDPVKVYLKEIGR
                     VPLLTPEEETELALKIQAGGPEGEKAKQRLSEANLRLVVSIAKRYVGRGMQFLDLIQE
                     GNLGLIKAVEKFDHTKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINKVKK
                     VSSQLLHECGHDPSAEEIAERLDMPVDKVREIMRVAQEPVSLETPIGEEEDSHLGDFI
                     PDDDAPVPAEAASQTLLKEQLADVLKTLTPREEKVLRLRFGLEDGRPRTLEEVGKEFN
                     VTRERIRQIEAKALRKLRHPSRSKKLRDFLD"
     gene            4568..4633
                     /locus_tag="ACZPAX_03610"
                     /pseudo
     CDS             4568..4633
                     /locus_tag="ACZPAX_03610"
                     /inference="COORDINATES: protein motif:HMM:NF024637.6"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
     gene            5402..5584
                     /locus_tag="ACZPAX_03615"
                     /pseudo
     CDS             5402..5584
                     /locus_tag="ACZPAX_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003456718.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
     assembly_gap    5701..5710
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5711..6240
                     /locus_tag="ACZPAX_03620"
     CDS             <5711..6240
                     /locus_tag="ACZPAX_03620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113017.1"
                     /translation="VEGLKEYITSTDGLTDSVLVSLQNQAEDVLKAYAANSFGDGETF
                     KGMSYLGNYILTKKNRDSWGDENMIILAYQVTVHDNYTSDNTGASYDGDNSYYWYISF
                     KNVSKNADGSIATGLDDYNTPSDQIVIDSGAQMYKFSDSTWSWYYHGYNTLDDLYSVA
                     VTQNVEDYNHQDNVG"
     gene            6386..>7636
                     /locus_tag="ACZPAX_03625"
     CDS             6386..>7636
                     /locus_tag="ACZPAX_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005360465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HIR1113018.1"
                     /translation="MKEKNRSSFSGKLGFVLSAAGASVGLGNIWRFPYLAAKYGGGIF
                     LLIYIVLALTFGYTMIMAETALGRMTHKSPVSAYGSFAKPGKKNGFLGFGGWINAIIP
                     VLIVPYYSVIGGWVIKYLFEYLRGNAQGLAQDDYFSAFIADGASAEICFLLFALFTLG
                     IIFAGVRNGVERVSRIMMPVLVVLSVIIAVYSITRPGALAGVAYFLVPNPDNFSWMTV
                     VTAMGQMFYSLSIAMGILVTFGSYMKKDVSIEESTEHVEIFDTIIAIMAGLMIIPAVF
                     AFSGGDPDTLQAGPALMFITIPKVFASMGLGTVVGVLFFLLVLFAAVTSSIALTESAV
                     STFEDELGWGRKKSTVLVGVIMVALGTLSSLGYGPLAGETVIGMQFLDFFDFLTNSVM
                     MPIAAIATCLLVSRVIGVEKIEQEV"
BASE COUNT         1717 a         2298 c         2211 g         1400 t
ORIGIN      
        1 tgaagccctt catctacacc tttgccatcg accacctcgg ccttacgccc tgcaccatgg
       61 ctcccaatct ccccgtctcg atcgagaccc cggcgggaat atggtcgccc aaggaggccg
      121 gcaaggtcga atatgacggt gtgctgcacc cgctctactg gggtatggcc aactcgcgaa
      181 acaactgctc ggcttggatc atgaagcagg ccaagcagcc cgcagccgtg aaaaaacttc
      241 aaaaaaattt ccacaaaaaa agagtgtttc ccgcagtttt gtcgtattta taaatagaag
      301 ctgtaaaaaa gggggacata ccatgacgat ccgcgaagag accgaagcca tcgagcgtca
      361 gaccttgagc cgttatgcaa cgttgagcga aaacagcctg ggccgccgca cgccgttgga
      421 ggatgacccc atccgcccct gtttccagcg cgaccgggac cgcattctgc actgcaaggc
      481 gttccgccgc ctaaagcaaa agacccaggt ctttctctca ccggagggcg accactaccg
      541 cacccgcttg acccacacgc tggaagtttc ccagatcgcc cgcacgatct cccgcgctct
      601 gcgcctgaac gaggatttga ccgaggccat cgccctgggc catgacctgg ggcacacgcc
      661 cttcggccac gcgggggagc gggcgctgaa tcgcctgtgc ccgggcggct ttacccacta
      721 ccgccaaagc ctgcgggtgg tggattactt agagcgcgac ggccagggcc tgaacctgac
      781 ctgggaggtg cgcaatggca tcgtgaccca cacgtcgggc gcgtgggccc gcacaccgga
      841 gggctgtgtt gtgcgccgcg ccgaccgcat tgcatttttg aaccacgaca tcgaggacgc
      901 tatcaccgcc ggtgtgctgg acgcccgcca gctgccgccc gaagccaccg ccgtgctggg
      961 cgcgacgaaa tcccagcgca tcacgacgat gataaccgat ctgatcgccc acagcgggga
     1021 aggacagatc aatttttcgc ccgaggtcga ggcagcctac gcgattttga aggacttcat
     1081 gtactcgacg atctacgttg acaaggaggc caagcgcgag gaaaaaaagg tggacaagct
     1141 catcagcgag ctgtacaccc gcctgagcga cgaaccggcc ctgatgccaa atttttatat
     1201 gcagatcgcc tacaacgagg gcgttgaccg cgccgtgacc gactacatca gcggcatgag
     1261 cgatgagttt gccacccgcc tgtttgagga actgttcgtg ccgcagaaat ggcacgtttt
     1321 gtaaccgctg cgccgtaggg gccgggcatg cccggccctg cacagattgg cagacgcagc
     1381 attgcgggaa aaccgcgggc cgggcatgcc cggcccctac aatctgacaa ggaggtgcag
     1441 tcaacccatg atcccccagg aatacataca ggaagtcgtg cggcgcaatg atattgaaga
     1501 gatcatcggc cagtatgtgc agctgcgccg ccgtgggcgc accgccaccg gactgtgccc
     1561 cttccataac gaaaaaacgc cgtccttcgt cgtttacccc gatacccaga gcttttactg
     1621 cttcggctgc ggcgcagcgg gcgacgtcat cagctttgtg cgcaagtaca ataacctcgg
     1681 ctatgtcgag gccgtcaaac agctggcctc ccgcgccgga atgcccctgc ccgaggagga
     1741 cgaccgcgaa tcccgcgccc gccagcgtct gctggaaatc aaccgctgcg ccgcgcggta
     1801 tttctacgag aacctcaacg ccaaaacgcc ggaggccgcc atggcccgcc gctactggaa
     1861 agagaagcgc ggcctgtccg acgcggccat ccgccgcttc ggcctgggct atgcgcccga
     1921 tgatttcgtc ggcctgctgc actatttaaa gcaccttggc ttctcggaaa gcgaactcga
     1981 aacttccggc cttgtcaagc gcagcgccaa gggcaatctc tacgatatct tccgccaccg
     2041 cgtcatggta cccatcatcg atgtgcgcgg ggctatcatc gcctttggcg gccgcgtgct
     2101 ggatgattct aagcccaagt acatcaacag cccggagacg atggtttacc acaaatcccg
     2161 cacgctgttt gcgctgaacg tcgcaaaaaa atccaagtcc aagcgctaca tcctctgcga
     2221 aggctacatg gacgttatct caatgcacga ggccgggttc gacacggcgg tctgtgcctg
     2281 cggaacggcc ctgacagccg agcaggccaa gctgctgagc gaatacgccg acgaggtcgt
     2341 attgagttac gactccgacg aggcgggcca gaaagccacc gagcgcagcc tgggcatcat
     2401 ggccaatacg ccgatgaaag tcagcgtgtt aagctaccag ggcgcgaagg accccgacga
     2461 gttcatcaaa aaatatggcc gtgagcggtt tgaaatgctc ttgaacggca cggccaatcc
     2521 caccgagttc cagctgaaaa aggcaaaatc caagtacgac ctgcgcagcg acgatgggcg
     2581 gttgagctac atccgcgagg ctatcgacat cctgaccgaa cgcggcgtca gccccacggc
     2641 acgggatgtc tacgccgggc ggcttgccga cgagaccggc gtctccaaac aggccatcgt
     2701 atcccagatg cagggcgcgg tgcgcaccgc cgagcgccgc aattaccgca aggaacagcg
     2761 cgatttatcc aaagagggca ttgccgctga tatccgtgtg ccctacacca gcggcggcga
     2821 gtctgccctg ggcgcggcca gtgcggcccg gcagctggcc gcagccctgc tgcaaaagcc
     2881 ggaggaagtg ccctacgtgc gcagccgcct ggatatggag atggtgcttg accctgaaat
     2941 gaaaacggtg ctgaacgcca tcttccgctg tgtggacgag cagctgccgc tgaacctgac
     3001 gaccttgcag cagatgctgg acgacaaagc cttcgggcag attgccctgg ctcaggcgca
     3061 gaaccacggt acgcccctgc aaagacagga tattgatatg tatctggacc gtatgcagac
     3121 cgccaagccc gccagcgaaa aggtcaagca gatggacgct gaccagttcg caaacttctt
     3181 tgccgatatc ggcaagcgca agggtgccca ccagccgacg cccgatgaat aacgccatcc
     3241 cacatcgttg ttgaacaata taaaaccaaa aggagaacct acttatggca gaaaagaaag
     3301 accccatccg caatctgatc gacagcggcc gccgtgcagg caagctgacc agcaccgaaa
     3361 tcagcgacgc tatggaagaa gccggcaacg tcctggaagt cgaagcgatg gaaaagctct
     3421 atgaggagct ggaaagcaac ggcattgagg tcatcgacga cacccccgtc gaggtggacg
     3481 acaccgttgc cgccccggat gacggcgccg acgattacga ggacggcctg accgccgacg
     3541 gcatcaccat cgacgacccc gtcaaagttt acttaaagga gatcggtcgc gtgccgctgc
     3601 tgacccccga ggaagaaacc gagctggcgc tgaagattca ggccggcggc cccgagggcg
     3661 agaaggccaa gcagcgtttg agcgaggcca acctgcgtct ggtcgtttcc atcgccaagc
     3721 gctatgtcgg ccgcggcatg cagttcctgg atctcatcca ggagggcaac ctgggtctga
     3781 tcaaggctgt ggaaaagttc gaccacacca agggcttcaa gttctccacc tatgcaacgt
     3841 ggtggatccg ccaggccatc acccgtgcca tcgctgacca ggcccgcacc atccgcatcc
     3901 cggtccacat ggtcgaaact atcaataaag tcaagaaggt gtccagccag ctgctgcacg
     3961 agtgcggcca cgaccccagc gccgaggaaa ttgccgagcg cctggatatg ccggtcgata
     4021 aggtccgcga gatcatgcgc gtagctcagg agccggtcag cctggaaacc cccatcggcg
     4081 aggaggagga cagccacctg ggcgacttca tccccgacga cgacgccccg gtccccgccg
     4141 aggccgccag ccagaccctg ctgaaagagc agttggccga tgtgctcaag accctgaccc
     4201 cgcgtgagga aaaggtcctg cgcctgcgct ttggcctgga ggatggccgc ccccgcacac
     4261 tggaagaggt cggcaaggag ttcaacgtca cccgtgagcg tatccgccag atcgaggcca
     4321 aggccctgcg caagctgcgc caccccagcc gcagcaaaaa actgcgtgac ttcctggatt
     4381 aagccccaaa ttgcggtatt tgggcctgaa tgtggtcatt catacatcaa aaacgacgtt
     4441 tcatccgaaa agggtggcaa actccaccct gatgatgtag aatcatgaca gaaagttctt
     4501 caaggaaagg ctgcaggcag cacccccaac aaggcgctgc ccgctgaaaa tcaaggagga
     4561 aaatagtatg atttgtgtac attgcggacg cgatatcccc aacggaacga agttctgccc
     4621 cttctgcggc cagccggtcg ccgctgacca gcccgccggt cagcctgcgt tcaacatcca
     4681 gcccggcgca agcgtgcagc cgccgcagca gcccccggtc atgggcgcac agcagcccat
     4741 gggcggccag cccgcagcgg ccaccgcaac ggtgacgccc aaagcaccca tcgaccccaa
     4801 gaagctggct gtgccggtcg ctgtggcggc tgtcgtggtt gtgggcggtg tgctgattgc
     4861 cacccataag cccaccgtca acctgaacaa gtacatcacc ctgtctgccg agggctacaa
     4921 ctccatcggc acgctggatg tcgagttcga caccgacaag ctggaaaagg attacggcaa
     4981 aaagattgcc aagaacttcc agaaggcgat gaagaaccac gaggaggaca cctacggcct
     5041 cagcaacctg gccggctccc tgtatgaggg cggcgagacg agcctgtttg tgacctactg
     5101 cgccgacggc agcgccgaca agacccgcaa cctgagcaac ggcgatgttg tcacctacac
     5161 ttgggacggc gtcaacgagc agaccaagaa ggaagccgag gagctgttcg gcgtcaagat
     5221 caagtgcagc gacgtgacct acaaggtctc cggcctgact gctgtgaata cctttgatgc
     5281 tttcgacggc gtcgaggtcg agttcgacgg catctccccg gacggcagcg ccacggtcaa
     5341 caccctgccc accgccgagg ccgccaaggg cttgtactac acgctggacg agcagtataa
     5401 cctcgccaac ggcgacaccg tcaccgtgat caaggacctc aaggtcaagg gctcctcgct
     5461 ggtggtcaag gtcggcacca aggtgaagaa catccgcctg gtcgacggcg atcacgacat
     5521 cgactgcaag atcgatggca tcggtgcgat aaagctcaag tccgagttcg tgcgcaaggt
     5581 ctgagccagt gcggaaaaac tgggttcttc gcgtattttg gggaaagtgc gggtgcacgg
     5641 ggttagccaa acaggccatc agtggcggca gatccttctg ccgggcaccg gtctgcacag
     5701 nnnnnnnnnn cggtggaagg actgaaggaa tacatcacga gcacggacgg actgaccgac
     5761 tccgtgttgg tcagcttgca gaaccaggca gaagatgttc tgaaagccta tgctgcaaat
     5821 agctttggtg atggtgaaac cttcaagggc atgagctacc tgggtaacta tatcctgacg
     5881 aagaagaacc gtgatagctg gggcgacgaa aacatgatta tccttgctta tcaggtgaca
     5941 gtccacgata attatacgag cgacaacacc ggtgccagct atgacggcga taattcttac
     6001 tactggtata ttagctttaa gaatgttagc aaaaatgctg acggcagtat cgcaactggt
     6061 ctggatgatt ataatacgcc cagcgaccaa attgtgattg attctggtgc gcagatgtat
     6121 aagtttagcg acagcacatg gagctggtac taccacggct ataacacgct ggacgacctg
     6181 tacagcgtcg ctgttaccca gaatgttgag gactacaacc atcaggacaa tgtcggctga
     6241 taacggcctg acaccaatct gaaaccaaca gccccgcctg cccggcgggg ctgttttgct
     6301 gccagggggt tgcattttgt gcttgtatgt gatacacttg cccacgtgta aaaaaactat
     6361 aatgcggagg gaaataggaa aaatcatgaa agaaaaaaac agaagcagct tctccggtaa
     6421 actcggcttt gtgctgtcgg cggccggggc atcggtgggg ctgggcaata tctggcggtt
     6481 cccgtacctg gcggctaaat acggcggcgg tattttcctg ctgatttata ttgtgctggc
     6541 gctcaccttc ggctacacga tgattatggc tgagaccgcc ctgggccgca tgacccacaa
     6601 aagtccggtc agcgcctacg gcagctttgc caagccgggc aagaaaaacg gctttctcgg
     6661 ctttggcggc tggatcaacg ccattatccc ggtcttgatc gtgccgtact actcggtcat
     6721 cggcggctgg gtcatcaagt atctgtttga gtatctgcgc ggcaacgccc agggccttgc
     6781 acaggatgac tatttcagcg ccttcattgc ggacggggcc tcggcggaga tttgctttct
     6841 gctgtttgcg ctcttcacgc tgggcatcat ctttgcgggc gtacgcaacg gcgtcgagcg
     6901 cgtctccagg atcatgatgc cggtgctggt ggtgctgtcg gtcattatcg cggtctactc
     6961 gatcacgcgg cccggcgcgc tggcgggcgt ggcctacttc ctggtgccca acccggacaa
     7021 cttctcctgg atgaccgtgg tcacggccat gggccagatg ttctactcgc tgtccatcgc
     7081 catgggcatt ttggtcacgt tcggttctta catgaaaaag gacgtctcga tcgaggaatc
     7141 caccgaacac gttgaaattt ttgacacgat catcgccatc atggcgggcc ttatgatcat
     7201 cccggcggtg tttgctttct ccggcggcga cccggacacc ttacaggcag gcccggcgtt
     7261 aatgttcatt accatcccca aggtgtttgc cagcatgggg ctgggcacgg tcgtcggtgt
     7321 gctgttcttc ctgctcgtac tgttcgcggc ggtcacaagc tcgattgccc tgaccgagag
     7381 cgctgtctcg acctttgagg atgagctggg ctggggccgc aaaaaatcca cggtgcttgt
     7441 cggcgtcatc atggtggcgc tgggtacgct gtccagcctg ggctatggcc cactggcggg
     7501 cgagaccgtc atcggaatgc agttccttga cttcttcgac ttcctgacca actcggtcat
     7561 gatgccgatt gcggccattg ctacctgcct gcttgtctct cgcgtcattg gcgtggagaa
     7621 aatcgagcag gaggtc
//
LOCUS       DBMDSQ010000063         7591 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_2590_length_7591_cov_1.548331, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000063 DBMDSQ010000000
VERSION     DBMDSQ010000063.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 7591)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7591)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7591
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2590_length_7591_cov_1.548331"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1470
                     /locus_tag="ACZPAX_03630"
     CDS             <1..1470
                     /locus_tag="ACZPAX_03630"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015537848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1113019.1"
                     /translation="DFRDQISKKINRLPLAYFEKRTVGEVLSRITNDVDTLGQSLNQS
                     VTQLITSVTTMIGVLIMMLTISPKMTLIALLILPVSLALVLLVVKFSQKYFKAQQATL
                     GVVNGQVEEVYAGHNVIKAFNREDAVLADFNAANDKLYESAWKSQFLSGLMQPIMNFV
                     GNLGYVAVAIVGSIFAAAGSITIGDIQAFIQYVKNFTQPIQQLAQVSNMLQSMAAAAE
                     RVFEFLNEEEENQQADPAHRANPACIDGQVTFDHVKFGYTPEKTVIHNFSCNVKPGQK
                     VAIVGPTGAGKTTMVKLLMRFYDVDAGEISLNGHNVRDFDRSALREGFGMVLQDTWLF
                     KGTIMENIRYGRLDATDEEVIAAAKAANADHFIRTLPGGYQMELNEDASNVSQGQKQL
                     LTIARTILADNRILILDEATSSVDTRTEQRIQTAMDRLMQGRTSFVIAHRLSTIRDAD
                     LILVMRDGDIVEQGTHDELIDQGGFYADLYNSQFEDVTA"
     gene            1716..2639
                     /locus_tag="ACZPAX_03635"
     CDS             1716..2639
                     /locus_tag="ACZPAX_03635"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HIR1113020.1"
                     /translation="MRKKLQNKRGFTLVELIVVLTILGVLAAMLIPALQGYIEKALET
                     SDLVNVRAAYMQVLSDAMTGDVTAEQTVQLKQGQDGWQHFDPVTIAGITHAADEGDTD
                     NWKGSPTAHGTCTVSYVDGTGVVLTWSGEGTKPSQPDRPTVNLSENLHEILAKTDLLK
                     TDLKDKDFYEIDSACPTSTMVPKVKEQIKKDSLLYTGTWDFRKIRSENPGRYLFWTSV
                     DVTKFAVGTEVPVLISKEHEGKFYITTMPVTTSKVKNQTYNVIGKGIDNRGTLKPYIT
                     GTEYTTLEDAYAAYADMVKNSYSQYADTLPR"
     gene            2750..3307
                     /locus_tag="ACZPAX_03640"
     CDS             2750..3307
                     /locus_tag="ACZPAX_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HIR1113021.1"
                     /translation="MDYAKLTAQARQATEELLEAAHLETGDIFVVGCSSSEIMGGHIG
                     KDSSMEAAAAVLAGVLPPLQEQGVYLAAQCCEHLNRAIVIERTVARANGYQIVAAIPQ
                     PHAGGSWATNCWRTFNDPVLVEEVKAAAGMDIGGTLIGMHLRRVAVPVRLSMDHIGQA
                     ILLCARTRPPFIGGSRAVYSQEEVR"
     gene            3304..4029
                     /locus_tag="ACZPAX_03645"
     CDS             3304..4029
                     /locus_tag="ACZPAX_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HIR1113022.1"
                     /translation="MIPEAQKQQMAEAVAKIRETMAAAARAAGRDPADVRLCAACKTR
                     TVEEVRYSADLPIDLFGENHVQELVEKTDAGAYNGKPGHFIGHLQTNKVNKVVGRAAL
                     IQSVDSTRLLDKIDAAAARLGLVQDILVEINIGEEASKSGVDADSLFPLLEAAAAREN
                     TRLRGLMAIPPADADDAETRRFFAQMRELLAKADAQHYDRAQMDILSMGMSHDYAAAI
                     AEGATIVRVGTAIYGARDYSKKA"
     gene            complement(4067..4927)
                     /locus_tag="ACZPAX_03650"
     CDS             complement(4067..4927)
                     /locus_tag="ACZPAX_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005938198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HIR1113023.1"
                     /translation="MPNQKPLRETKQHGAAWFPFNIYPCTIPQDFPQVALHWQDSMEL
                     VFVKKGRGVVQAGLTTYTAEAGDVYVFAPGVLHALRQLPGEVMEYENIIFDLELLGGA
                     GDLCAEKYLLPLQSGRLALPMRLAPNDWGYTWAVNCLHAAEEFNKLKDVGYELCIKGE
                     LLLFLAALVGRSGDLPPADTPDTRRLKTVVQLVNEQYAGVLHVTDAAAACGCSPSHFM
                     RWFKKMTGQGFTEYLNEHRLNAAADALRSSNDTILAIAEQCGFDNLSYFNRLFKKRYG
                     LTPREYRVRK"
     gene            5116..7377
                     /locus_tag="ACZPAX_03655"
     CDS             5116..7377
                     /locus_tag="ACZPAX_03655"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008719694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HIR1113024.1"
                     /translation="MNMTTVIEAKTNKPLASCTDQEIYLALLDIVREQSAAHVKPVKG
                     RKLYYISAEFLIGKLLSNNLINLGLYDDTRAALAAAGKNLSDIEEVEPEPSLGNGGLG
                     RLAACFLDSLATLDLPGDGVGLRYHFGLFHQSFKDGVQNELPDPWLTAHSWAEKTDTV
                     YPVELAGKTYNARLYKLAVTGYEGRTNTLNLFDLDTIDETIVHDGITFDKTAIDKNLT
                     LFLYPDDSDEAGRRLRVYQQYLMVSAGAQLILAECAARGCDYHDLADYAAIQINDTHP
                     SMVIPELIRLLGEKGIEFDEAVEIVTKTCAYTNHTILAEALEKWPRAYLDAVVPQLMP
                     IIEKLDELARTRTKDESLAIIDKDDRVHMAHMDIHFTHSTNGVAYLHTEILKNTELHG
                     FYELYPEKFNNKTNGITFRRWLLECDPALTAEIEKLIGSGFRKDATELEKLLPYAENV
                     DTLQEFSAVKSQNKQALADWLKRTQNVTIDPNAMFDIQSKRLHEYKRQQLNLLYLIHQ
                     YYEIKAGHLPATPLVSIFGAKAAPAYIIAKDIIHALLTLSKVIAADPVVSKHLQVVFV
                     ENYNVTAAEKLIPACDLSEQISLASKEASGTGNMKFMLNGALTLGTMDGANVEISQQV
                     GNDNIYIFGQTSDQVIHRYDAGDYCAAQWYEGDPNIRRAIDFLTSPEMLAAGHEENLK
                     RLHDELINKDWFQTLPDFNAYVVRKGQALSDYACDPLGWRKKCVINIAKAGMFSSDRT
                     IAEYNDDIWHLGE"
BASE COUNT         1723 a         2308 c         2098 g         1462 t
ORIGIN      
        1 gatttccgcg accagatcag caaaaaaatc aaccgtctgc cgctggccta ctttgaaaag
       61 cgcaccgtcg gcgaggtact gtcccgcatc accaacgatg tggacacgct gggccaaagc
      121 ctgaaccaga gcgtgaccca gctcattacc agcgtcacga cgatgattgg cgtgctgatc
      181 atgatgctca ccatcagccc aaagatgacg ctgattgcgc tgctgatcct gccggtgtcg
      241 ctggcgctgg tgctgctggt cgtcaaattc agccaaaaat acttcaaggc gcagcaggca
      301 acgctgggcg ttgtcaacgg ccaggtcgag gaagtctacg ccggccacaa cgttatcaag
      361 gcgttcaacc gtgaagacgc tgtgctggca gacttcaacg ccgccaatga caaactgtat
      421 gaatccgcgt ggaaatccca gtttttgtcc ggcctgatgc agcccatcat gaactttgtg
      481 ggcaacctgg ggtatgtggc ggtcgccatc gtcggcagca tttttgcagc ggcgggcagc
      541 atcaccatcg gcgatatcca ggcgttcatc cagtatgtca agaacttcac ccagcccatc
      601 caacagctgg cgcaggtgtc gaatatgctg caaagcatgg ctgccgcagc cgagcgcgtc
      661 tttgaatttt tgaacgaaga agaggagaac cagcaggccg accccgccca ccgcgcgaat
      721 cctgcctgca tcgatggcca ggtcacgttt gaccacgtca aattcggcta cacgccggaa
      781 aagacagtca tccacaattt cagctgcaac gtcaagcccg gccagaaggt cgccatcgtc
      841 ggccccaccg gcgcgggcaa aaccacgatg gtcaagctgc tgatgcggtt ctacgatgtg
      901 gatgcgggcg aaatcagcct gaacggccac aatgtccgcg actttgaccg cagcgccctg
      961 cgcgagggct ttggtatggt cttgcaggat acctggctgt tcaaaggcac catcatggag
     1021 aacatccgct atggccgcct ggacgccacc gacgaggaag ttatcgctgc cgccaaggcc
     1081 gcgaacgcag accactttat ccgcacgctg cccggcggct accagatgga gctgaacgag
     1141 gacgcctcca acgtcagcca gggccagaaa cagcttttga cgattgcccg cacgattctg
     1201 gcggacaacc gcatcctgat tctggacgag gcaacgtcca gcgtcgatac ccgcaccgag
     1261 cagcgcatcc agacggccat ggaccgcctg atgcagggcc gcacaagctt tgtcattgcc
     1321 caccgtctgt ccactatccg cgacgcagat ctgattctgg tcatgcgcga cggcgatatt
     1381 gtggagcagg gaacccatga cgagctgatc gatcagggcg ggttctacgc ggatctgtat
     1441 aatagtcaat ttgaggatgt aacggcgtaa aggcttccgc ctttggggga aagtcgtcac
     1501 cgcaggtgac tgagggggcc tgccacctgc cggacattgc cccctcattc ggtgcactgt
     1561 ccgccctgca gcatcaaaat gtaaataatt tgtaaaatac aggtaaaacc tgcataaaaa
     1621 gcgaaaaatc atttgcgcat cgacaaaaac tgtgttacaa tgtaatgtat ttattctgga
     1681 attgtgtgca tataacacga taaggtgcgg aacgaatgag gaaaaaactg caaaataagc
     1741 gcggctttac gctggtggag ctgatcgtcg tgctgaccat acttggggtt ttggcggcga
     1801 tgctgatccc cgcgctgcaa ggctatattg aaaaagcgct ggaaacgtcg gatcttgtca
     1861 acgtgcgcgc cgcgtatatg caggtgcttt ccgatgcaat gaccggcgat gtcactgccg
     1921 aacagaccgt acagctcaag caggggcagg acgggtggca gcactttgac cccgtcacca
     1981 ttgcgggcat cacccacgct gccgacgagg gtgacaccga caattggaaa ggctcgccca
     2041 cggcacacgg aacctgcacg gtttcttatg tggatgggac aggcgtggtg ctgacgtggt
     2101 caggcgaagg caccaagccg tcgcagcccg accggccgac cgttaatttg agcgaaaacc
     2161 tgcacgagat tttggcaaaa accgacttgc tgaaaacgga tttaaaggac aaagattttt
     2221 atgagattga ctccgcctgc ccgacctcca cgatggttcc caaggtgaag gagcaaatca
     2281 agaaagacag cctgctgtac accggaacgt gggatttccg caagattcgc agcgaaaacc
     2341 ccgggcggta tttgttctgg acatcggtgg atgtgactaa atttgcagta ggcacggagg
     2401 tgccggtcct tatcagcaaa gaacacgagg gcaaatttta catcaccacg atgccggtga
     2461 cgacctcaaa ggtgaagaat cagacctaca acgtgatcgg caaaggcatt gacaaccgcg
     2521 ggacgttgaa accctatatc acgggtaccg agtacaccac gctggaggat gcctatgccg
     2581 cgtatgcgga tatggtgaaa aacagttatt cgcagtacgc ggataccctg ccaaggtaga
     2641 aatgcctcca accgcccaac gggcaaactg catatatgcc ttccccctca ggggaaggtt
     2701 tttttgttat tgacacccct gctataatag taccaaaggg ggctttttca tggattacgc
     2761 aaaacttacc gcacaggccc ggcaggctac tgaagaactg ctggaagccg cacacctgga
     2821 aactggggat atcttcgtgg tcggctgctc cagcagcgag atcatgggtg gacatatcgg
     2881 aaaggattcc agcatggagg ctgctgccgc cgtgctggcg ggtgtgctgc cgcccttgca
     2941 ggagcagggc gtctaccttg ctgcccagtg ctgcgagcac ctgaaccgcg ctatcgtcat
     3001 cgagcgcacg gtcgccaggg ccaacggcta tcaaatcgtg gcggccatcc cgcagcccca
     3061 cgcgggcggc agctgggcta ccaactgctg gcgcaccttt aacgatccgg tgctcgttga
     3121 ggaagtcaaa gctgcagcgg gcatggacat cggcggcacg ctcatcggga tgcacctgcg
     3181 ccgcgtggct gtgccggtgc gcctgtcgat ggatcacatc gggcaggcga ttctgctctg
     3241 cgcccgcacc cgcccgccgt tcatcggcgg ctcccgcgcc gtttacagtc aggaggaagt
     3301 gcgatgatcc cagaagcaca aaaacagcag atggccgagg ccgtcgcaaa gatccgggag
     3361 acgatggctg ccgccgccag ggccgcaggc cgcgaccctg ccgacgtgcg cctttgtgcc
     3421 gcctgcaaaa cccgcacggt ggaagaagtt cggtacagcg ccgatttgcc cattgacctg
     3481 ttcggcgaaa accacgtaca ggagcttgtg gaaaagaccg acgccggagc ctacaatggc
     3541 aagcccggac attttatcgg tcatttgcag accaataaag tcaacaaggt tgtaggccgt
     3601 gccgcgctca ttcagagcgt ggacagcacg cggctgctcg ataaaatcga cgctgccgcc
     3661 gcgcggctgg gtctggtgca ggacattttg gtggaaatca acatcggcga ggaagccagc
     3721 aaaagcggcg tcgacgccga cagcctgttc ccgctgctgg aagctgccgc cgcgagggag
     3781 aatacccgcc tgcgcggtct gatggcgatt ccgcccgctg acgcagacga tgccgagacg
     3841 cgccgctttt ttgcgcagat gcgggagctg ctggccaagg ccgatgccca gcactacgac
     3901 cgggcgcaga tggatatttt gtccatgggt atgagccacg actacgctgc cgccatcgcc
     3961 gagggcgcga ccatcgtccg cgtaggcacc gccatctacg gcgcacgaga ttacagtaag
     4021 aaagcataaa attaccgccg gcatctcgtt gatgccggcg gcttttttat ttcctcaccc
     4081 ggtattccct cggtgtcagg ccatatcttt ttttaaataa cctgttaaaa tacgacaagt
     4141 tgtcaaaccc gcactgctcg gcaatcgcca gtatcgtgtc attgctgctg cgcagggcgt
     4201 cggcggcggc gttcaggcgg tgctcgttca ggtattcggt aaagccctgg ccagtcattt
     4261 tcttgaacca ccgcataaaa tggctagggc tgcacccgca ggctgccgct gcgtcggtca
     4321 cgtgcagcac gcccgcgtac tgctcgttca ccagctgtac cacggttttc aaacgtcggg
     4381 tgtcgggggt atcggcaggg ggcaggtcgc cgctgcggcc caccagcgcc gctaaaaaca
     4441 acagcagttc accctttata cacagctcat agccaacatc ctttaacttg ttgaactcct
     4501 ctgccgcgtg cagacagttt acggcccagg tataacccca gtcgttgggg gccagccgca
     4561 tgggcagcgc caggcggccg ctttgcaggg gcagcaggta tttttcagcg cacaggtcgc
     4621 ccgcaccgcc cagcagttcc aaatcaaaga tgatgttctc atattccatc acctcgcccg
     4681 gcagctggcg cagcgcgtgc agcacgccgg gggcaaacac atagacatcg cccgcctcgg
     4741 cggtgtaggt cgtcaggccc gcctgcacga cgccgcgccc ttttttgaca aacaccagct
     4801 ccatgctgtc ctgccagtgc agcgccacct ggggaaaatc ctgcgggatc gtgcagggat
     4861 agatgttgaa agggaaccac gcggccccgt gttgctttgt ttcgcgcaag ggtttctgat
     4921 ttggcatgat tttcacctcg ttgatagtat tgtaccacta ttggccatta ttttgcaagc
     4981 atcatgcgcg ttttgtgtgg tataacctag gtgtagtcaa gaaatatacc tattggccca
     5041 aaagttccgg gccaaggcaa ggctcaaccc ttccgcccct gccaggggag gcaagtatat
     5101 gaggaggtaa ctctcatgaa tatgaccact gtgatcgaag ccaaaaccaa caagccgctg
     5161 gcatcctgca ctgaccagga aatctacctt gccctgctgg acatcgtccg cgagcagagc
     5221 gccgcccacg tcaagcctgt caagggccgc aagctctact acatcagtgc cgagttcctg
     5281 attggcaagc tgctgtccaa caaccttatt aacctgggtc tctacgacga cacccgcgcc
     5341 gcgctggccg ctgcgggcaa gaacctgtcc gacattgagg aagtcgagcc ggagccgtcc
     5401 ctgggcaacg gcggtttggg ccgcctggct gcctgcttcc tcgactccct cgccacgctg
     5461 gatctgcccg gcgacggtgt cggcctgcgc tatcacttcg gcctgttcca ccaatccttc
     5521 aaggacggcg tccagaacga gctgcccgac ccctggctga ccgcacactc ctgggccgaa
     5581 aagactgaca cggtctatcc ggtcgagctg gcaggcaaaa cctacaatgc ccgcctgtac
     5641 aagctggccg tcaccggtta tgagggccgc accaacacgc tgaacctttt tgacctggac
     5701 accatcgatg agactatcgt ccacgatggc atcaccttcg ataagaccgc catcgacaag
     5761 aacctgacgc tgttcctgta ccccgatgac tccgacgagg ccggccgccg cctgcgcgtc
     5821 taccagcagt atttgatggt ttccgccggt gcccagctga ttctggccga gtgcgccgcc
     5881 cgcggctgcg attaccatga cctggccgac tatgccgcca tccagatcaa tgacacgcac
     5941 cccagcatgg tcatccccga gctgatccgc ctgctgggcg agaagggcat cgagttcgac
     6001 gaggccgtcg agatcgtcac caagacctgc gcctacacca accacacgat tctggccgag
     6061 gcgctggaaa agtggccccg cgcctatctg gacgccgtgg tcccgcagct catgcccatc
     6121 atcgagaagc tggatgaact ggcccgcacc cgcaccaagg acgagagcct tgccatcatc
     6181 gataaggacg accgcgtcca tatggcccac atggacatcc actttaccca ctccaccaac
     6241 ggcgttgcct acctgcacac cgaaattttg aagaacaccg agctgcacgg cttctatgag
     6301 ctgtaccccg agaagttcaa caacaagacc aacggcatca ccttccgccg ctggctgctg
     6361 gagtgcgacc ccgcactgac cgccgaaatt gaaaagctca tcggttccgg cttccgcaag
     6421 gacgccaccg agctggaaaa gctgctgccc tatgccgaaa acgtcgatac cctgcaggag
     6481 ttctccgccg tcaagtccca gaacaagcag gcactggccg attggctcaa acggacccag
     6541 aacgtcacca tcgacccgaa tgccatgttc gatatccagt ccaagcgcct gcatgagtac
     6601 aagcgtcagc agctgaacct gctctacctc atccaccagt attacgagat taaggctggc
     6661 cacctgcccg ccaccccgct ggtcagcatc tttggtgcca aggccgcccc ggcatatatc
     6721 atcgcaaagg atatcatcca cgcactgctg acgctgtcta aggtcattgc cgctgacccg
     6781 gtcgtttcca agcatctgca agtcgtcttt gtggagaact ataacgtcac cgccgcagaa
     6841 aagctcatcc ccgcctgcga cctgtccgag cagatttccc tggcatccaa ggaagcctcc
     6901 ggtaccggca acatgaagtt catgctgaac ggtgcgctga ccttgggcac gatggacggc
     6961 gcaaacgtcg aaatcagcca gcaggtcggc aacgacaata tctacatctt cggccagacg
     7021 tccgaccagg tcatccaccg ctatgatgcg ggcgactact gcgccgccca gtggtacgag
     7081 ggcgacccca acatccgccg cgccatcgac ttcctgacca gccccgaaat gctggccgcc
     7141 ggtcatgagg aaaacctcaa gcgtctgcac gacgaactca tcaacaagga ttggttccag
     7201 acgctgcccg acttcaacgc ctatgtggtg cgcaagggcc aggccctgag cgactatgcc
     7261 tgcgacccgc tgggctggcg caagaagtgc gttatcaaca ttgctaaggc cggtatgttc
     7321 agcagtgacc gtaccatcgc cgagtacaac gacgatatct ggcatctggg cgagtaattt
     7381 gccgccaata atcgagtagt cggctctcct taaagagccg acctctcaca ccaccgttcg
     7441 taccgaccgg tacacgacgg ttcaaccgca taagtgcaaa gactcgtagt agttgaggat
     7501 actaaagtat cccgctgctg cgagtctttc gtttgtaatg gaacacttta aatcaggact
     7561 accggcgata cgccagtatc ccagtctgga g
//
LOCUS       DBMDSQ010000064         7553 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_2607_length_7553_cov_2.588567, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000064 DBMDSQ010000000
VERSION     DBMDSQ010000064.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 7553)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7553)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7553
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2607_length_7553_cov_2.588567"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            357..944
                     /locus_tag="ACZPAX_03660"
     CDS             357..944
                     /locus_tag="ACZPAX_03660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113025.1"
                     /translation="MGFFFKKANKANRCLLCGSEVATGKCTACGREAKPLVPLSALNW
                     QKVPLSVGEALGEQKKKLFGNALLTVKEMIANGELYIADIHIDGVYEHEYDSTPDEED
                     CRTEYDYYIQFSTPDLAPCDTDCEVSEGDYDRAEALLKSGRHDAKILWGRKKKANYYY
                     AFVPRNSDAAAMLTKGLFEKFIGFGECTEKRKLPY"
     gene            1027..1413
                     /locus_tag="ACZPAX_03665"
     CDS             1027..1413
                     /locus_tag="ACZPAX_03665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113026.1"
                     /translation="MGLGEMMGVKNVKLELKGNYATLADLLEAMKDIQFEAGVPELTK
                     HGIGAVIVFPPVDRNNQVWITGAKGKFTIMRSAEVAGLGNMAKNAVLDTLTDGWSSMS
                     GAFGNKKKRCMELVDITAKEIKEAEI"
     gene            1517..2512
                     /locus_tag="ACZPAX_03670"
     CDS             1517..2512
                     /locus_tag="ACZPAX_03670"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008720 - D-lactate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0070404 - NADH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HIR1113027.1"
                     /translation="MKIIFYGTREYDHYYFDVLAADPEYGCEIRFLTANLDADTAPLA
                     QGGDAVCAFVNADCSAPVLEKLARVGIRCLLLRCAGYNHVDLAAAKKHGITVLRVPGY
                     SPEAVAEHAMALAQAANRRLCKAYIKVRNNNFALDGLLGYNLYGSSAGIVGTGRIGAA
                     MARICHGFGMTVLAYDQYKNPALDGIVRYVELDELLRTADLISLHCPLTAETYHMINA
                     DTIKMMRGSAILVNTSRGGLIDTDALIDALRLRKFAGVGLDVYEDEDEQVFEDFSDDV
                     LQNEVVPVLLSFPNVVVTSHQAFFTRTALQSIAITTMENARAFARGEKLVNEVKG"
     gene            complement(2722..3819)
                     /gene="uxuA"
                     /locus_tag="ACZPAX_03675"
     CDS             complement(2722..3819)
                     /gene="uxuA"
                     /locus_tag="ACZPAX_03675"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005931401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HIR1113028.1"
                     /translation="MSMKMGWRWYGEGNDPITLSDIKQIPGVEEIVWALHSKMPGEIW
                     EENEIKEVVDQIHAAGFSATVVESVNVHDDIKIGLPTRDKYIENYKQCIRNLSKFGVK
                     TICYNFMPIFDWTRTDLFHPVGDGSTALFYQKDLIKDDYKGMANYILEFTEKYNMTFP
                     GWEPERMAKLDELFKAYAPVTKEKLWDNLKYFLEAIMPTCRECDIKMAIHQDDPPWDI
                     FGLPRLLVDAESIDRFLTMVDDPYNCLTLCSGSMNANPNNNVAAIVRKHCDRIPFAHV
                     RNIHHFENGDFSEAAHRDCCGETGIIEILRAYHDCGFDGCIRPDHGRQLWEEGPGNCR
                     PGYGKYDRALGVQYMLGVWDLLDRLDEEKNR"
     gene            complement(3997..5613)
                     /locus_tag="ACZPAX_03680"
     CDS             complement(3997..5613)
                     /locus_tag="ACZPAX_03680"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007048776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HIR1113029.1"
                     /translation="MQMNASSIVNQKAEWEKLGVKLPAFDHAAMTAATKEHPIWVHFG
                     AGNIFRGFIAALQQRLLNEGAADRGIIAADTFDYDIIDKIYTPYDNLTMNVTLNPDGT
                     TSREIIGSVAEGLRANSADAEMMARFKQIFTDPGLQMISFTITEKGYALYRPDGSLMP
                     VVQADIDEGPAHARHAMSMVAALLLERFQNGAAPLAVVSMDNCSHNGEKLQASVMTVA
                     KAWLEKGYVGQDFIDYLSDESKITFPWSMIDKITPRPHKIVEESLEKDGIEGMTPIVT
                     SKNTFIAAFVNAERPQYLVVEDKFPNGRPALEKAGVYMTDRETVNKTERMKVTTCLNP
                     LHTAMSVYGCMLGYDLICAEMKDEDIVALIKRLGYVEGLPVVVDPKILDPKAFIDEVI
                     EQRLPNPFMPDMPQRIATDTSQKVGIRFGETIKSYIAEGRDLNSLVSIPLAIAGWLRY
                     LLAIDDNGNAFEVSADPLKDDLQAKLAGIEFGKPETVTDQLDGILSNASIFGSDLTKT
                     VLADKIKAYFKAEIAGPGAVRKTLNEAVNG"
     gene            5913..6776
                     /locus_tag="ACZPAX_03685"
     CDS             5913..6776
                     /locus_tag="ACZPAX_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009324405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1113030.1"
                     /translation="MSNYNPYTKRQTMIAPDYEVYRYRSTYMNEVELHHHDFYEVYLL
                     LRGRVEYIVENQLYRVRPGDWMLCSPLELHQARIATDADAYERIVLWIARPFLENLST
                     PHTSLTRCFDTSIPSHTNLLRLPGATGAPLRTTVDKLCALKASKDYGSDLLAQSALVE
                     LMVGLNRAAAERGDARPTGTSDQVVDAVLHYINEHYSEALTLDQLSEKFFISKYHLLR
                     KFDAQVGTTVHRYILQKRLLNAKQLLAGGVPPNEVCQYCGFGDYANFYRAFRAEYNQT
                     PRQYIQSARGK"
BASE COUNT         1581 a         2030 c         2384 g         1558 t
ORIGIN      
        1 ggaaagacag ttagtgccat tgcctggcaa gtgataattt aaccaaatac tatttcaaaa
       61 atttggacga tttgacaatg attttttacg ctcttcaaat gatgaggcag aagtggtatg
      121 attatcataa tcaaggaaac cgcggccggg agatcaatct attgtaagga cgtaaagggg
      181 atgcgttatg ggcaaactgg ggaagatacc ccgcaacata ctgatcgtct gcggtgcgct
      241 ttttatcctt ctgggatgcg gccgtccaga tggattttat ggatgtcatg attatgtatg
      301 cgcggctgtt cgacgaagcg gacttctggc tgcggaatta acaaggaggc tatcatatgg
      361 gattcttttt caaaaaggca aacaaagcca accgctgcct gctctgcggc agtgaggtgg
      421 ccacgggtaa atgtacggct tgcggacgtg aggccaaacc gctggtgccg ctttccgctc
      481 tgaactggca aaaggtaccc ctcagtgtag gcgaagccct cggcgagcag aaaaagaagc
      541 tgtttggcaa tgcgctgctt accgtaaaag aaatgattgc aaacggcgaa ctttatatcg
      601 cggatattca catcgatggt gtgtacgagc acgaatatga cagcaccccc gatgaagagg
      661 attgccgcac cgagtatgac tattacatcc agttctctac cccggacctt gccccctgtg
      721 acacagattg cgaggtctcg gagggcgact acgaccgagc cgaagcgttg ctgaaaagcg
      781 gccggcatga cgctaagatc ctgtggggcc gcaagaagaa ggccaactat tactatgcat
      841 ttgtgccgag aaacagcgat gcggcggcca tgctgacaaa aggcctgttc gaaaaattca
      901 tcggctttgg cgaatgcacc gaaaagcgca agctgcccta ctaaaagggg agcaaatctg
      961 tttgaaacaa tatgtccaga aagctctgtg agcttacaac atagaaatca aaaagaaggt
     1021 cactatatgg gacttggtga aatgatgggc gtgaagaacg tcaagctgga gctgaaaggg
     1081 aattatgcta cactggcaga cctgttagag gcgatgaagg acatccaatt tgaagctgga
     1141 gttccggaac tcacgaagca tggcatcggt gcggtaattg tattcccgcc ggttgaccgt
     1201 aacaaccagg tgtggatcac gggtgcaaag ggaaaattca ccatcatgcg cagtgcagag
     1261 gtggctggcc ttggcaacat ggcgaagaat gccgttcttg ataccttgac cgacggctgg
     1321 agcagcatga gcggcgcttt cggcaacaag aaaaagcgct gcatggaact ggtggacatt
     1381 actgccaaag aaatcaaaga agcagaaatt tgagcctgta tatagaggtg ttgtttcgac
     1441 aacagaaatc ccccggtcta tccggtatac tatgcctgta aacggcagat actggatatg
     1501 actgggggat tttttaatga aaattatttt ttatggcacg cgggaatacg accactacta
     1561 ttttgatgtg ctggcagcgg atccggaata tggctgcgaa atcaggttcc tgacggccaa
     1621 tctggacgct gacaccgcgc cgctggcgca gggcggtgac gcggtgtgtg catttgtcaa
     1681 tgcggactgc tcggcaccgg tgctggaaaa gctggcccgg gtgggcatcc gctgcctgct
     1741 gctacgctgc gccgggtaca atcatgtgga cctggcggcg gctaaaaagc acggtataac
     1801 ggtgcttcgt gtgccggggt acagcccgga ggccgtggcc gagcacgcca tggcgctggc
     1861 tcaggcggcc aaccggcggc tgtgcaaggc gtatatcaag gtgcgcaaca acaattttgc
     1921 gctggacggc ctgctggggt acaaccttta cggctcgtcg gcgggcatcg tcggcaccgg
     1981 gcgcatcggc gcggccatgg cccgtatctg ccacggattt gggatgacgg tgctggccta
     2041 cgatcagtac aagaacccgg cgctggacgg tattgtgcgc tatgtggagc tggacgagct
     2101 gctgcgcaca gccgacctca tcagcctgca ctgcccgctg acagcggaaa cctaccacat
     2161 gatcaacgca gatacaatca aaatgatgcg tggcagtgca attttggtca acacgagccg
     2221 gggcggactg atcgacaccg acgcgctgat cgacgcgctg cgtctccgca agtttgcggg
     2281 tgtcgggctg gacgtctatg aggacgagga cgaacaggtg tttgaggatt tttccgacga
     2341 tgtattgcag aacgaggtcg taccggtttt gctgagcttc ccgaatgtgg tggtgacgag
     2401 ccatcaggcg ttttttaccc ggaccgcgtt acagagcatt gccattacga cgatggagaa
     2461 tgcgcgggcg tttgcgcgag gggaaaagct ggtgaatgag gtgaaggggt aagcagggca
     2521 gaagggcggt aaaggcaaag ctgcgggaat ccctccggcc tcgcaggctc ggccacctcc
     2581 ctttgacaag ggaggcgcgg gcggcatata tgccgcccct accgtggcgg cccgcggcgg
     2641 agttgattcc ccaaaacaaa aaagccactg cttgcgcagc ggcttttctg tttttataca
     2701 gcaactagag gatggcagaa atcagcggtt cttctcctcg tccagacgat ccagcaggtc
     2761 ccagacgccg agcatgtact ggacacccag cgcacggtca tacttgccgt agccggggcg
     2821 gcagttgccg gggccttcct cccacagctg gcggccgtgg tcggggcgga tgcagccgtc
     2881 aaagccgcag tcgtggtaag cgcgcaggat ctcgatgatg ccggtctcgc cgcagcagtc
     2941 gcggtgggca gcctcagaga agtcaccgtt ctcgaagtgg tggatgttgc gcacatgggc
     3001 gaaggggatg cggtcgcagt gcttgcgcac gatggcggcc acattgttgt tggggttggc
     3061 gttcatgctg ccggagcaga gcgtcaggca gttgtacggg tcatcgacca tcgtcaggaa
     3121 gcggtcgatg ctctcggcgt caaccagcag gcggggcagg ccgaagatat cccatggggg
     3181 atcgtcctga tggatggcca tcttgatgtc gcactcgcgg caggtgggca tgatcgcctc
     3241 gaggaagtac ttcaggttat cccacagctt ctccttggtg acgggggcgt aggccttgaa
     3301 cagctcgtcc agcttggcca tgcgctccgg ctcccagccg gggaaggtca tgttgtactt
     3361 ctcggtaaat tcgagaatgt agtttgccat gcccttgtag tcgtccttga tcaggtcctt
     3421 ctgatagaac agggcggtgg agccgtcgcc gacggggtgg aacaggtcgg tgcgcgtcca
     3481 gtcgaagatg ggcatgaagt tgtagcagat ggtcttgacg ccgaacttgg acaggttgcg
     3541 gatgcactgc ttgtagttct cgatgtactt atcgcgggtg ggcaggccga tcttgatgtc
     3601 gtcgtggacg ttgacggact cgacgacggt ggcgctgaag ccagccgcgt ggatctggtc
     3661 tacgacctcc ttgatctcgt tctcctccca gatctcgccg ggcatcttgc tgtgcagcgc
     3721 ccagacgatc tcctcaacac cgggaatctg cttgatgtcg ctcagggtga tggggtcatt
     3781 gccctcacca taccagcgcc aacccatttt catagacata tggtgctcct tttgtttgta
     3841 tagtcgtttg cgtaggggcg aggcatgcct cgccctcaag tttccggttg caagcccgtg
     3901 agcgggttat agattttatg cggcgggccg gacatgtccg gcccctacgg ggcggcagat
     3961 acaaggcttc ctccttaggg gaagccttta agcagcttag ccgttcacag cctcgttcag
     4021 ggtcttgcgg acggcgccgg ggccggcgat ctcggccttg aagtaggcct tgatcttgtc
     4081 ggccagcacg gtcttggtca ggtcgctgcc gaagatggaa gcgttagaca ggatgccgtc
     4141 cagctgatcg gtgacggtct cgggcttgcc gaactcgatg ccggcaagct tggcctgcag
     4201 gtcgtccttc agcggatcag cggaaacctc aaaggcgttg ccgttgtcgt caatggccag
     4261 caggtagcgc agccaaccgg cgatagccag aggaatggaa acgaggctgt tcaggtcgcg
     4321 gccctcggcg atgtagctct tgatggtctc gccgaagcgg atgccgacct tctgagaagt
     4381 atcggtggcg atgcgctgcg gcatatcggg catgaagggg ttgggcaggc gctgctcaat
     4441 gacctcgtca atgaaggcct tggggtccag gatcttcggg tcaacgacga ccggcaggcc
     4501 ctcaacgtag cccaggcgct tgatcagggc gacgatgtcc tcgtccttca tctcggcgca
     4561 gatcaggtca tagcccagca tgcagccgta aacgctcatg gcggtgtgca gggggttcag
     4621 gcaggtggtg accttcatgc gctcggtctt gttgacggtc tcgcggtcgg tcatgtagac
     4681 gccggccttc tccagtgcgg ggcggccgtt ggggaacttg tcctcaacaa cgaggtactg
     4741 cgggcgctcg gcgttgacga acgcggcgat gaaggtgttc ttggaggtga cgatgggggt
     4801 catgccctcg atgccgtcct tctccaggga ctcctcgacg atcttgtggg ggcgcggggt
     4861 gatcttgtcg atcatggacc aggggaaggt gatcttgctc tcgtcagaca ggtagtcgat
     4921 gaagtcttgg ccgacatagc ccttttcgag ccaagccttg gcaacggtca tgacgctggc
     4981 ctgcagcttc tcgccgttgt gagagcagtt gtccatgctg accacggcca gcggggcggc
     5041 gccgttctgg aaacgctcca gcagcagggc ggcgaccatg ctcatggcgt ggcgtgcgtg
     5101 ggcagggccc tcgtcgatgt cagcctgcac gacgggcatc agggaaccat cgggacgata
     5161 cagggcgtag cccttctcgg tgatggtgaa ggagatcatc tgcaggccgg gatcggtgaa
     5221 gatctgcttg aagcgggcca tcatctcggc gtcggcagag ttggcgcgca ggccctcggc
     5281 cacagagccg atgatctcgc ggctggtggt gccgtccggg ttcaaggtga cgttcatcgt
     5341 caggttgtcg tagggagtgt agatcttgtc gatgatgtcg tagtcgaaag tgtcagccgc
     5401 gatgatgccg cggtcggcgg caccctcgtt cagcaggcgc tgctgcaaag cagcgataaa
     5461 gccgcggaag atgttgccgg caccgaagtg gacccagatg gggtgctcct tggtagcagc
     5521 ggtcatggcg gcgtggtcaa atgcgggcag cttgacgccc agcttttccc actcggcctt
     5581 ctggtttacg atggaagacg cattcatctg cataatgatt tgctcctttg ttgcttatgg
     5641 gggtgggcag cgcagaactg cccgcgatat caacagtata ccacgcaatt ttttgcaaga
     5701 aaataaccgt agttgcttga aaaattgtaa aaattgcggt aaactaaacc tagcatcgaa
     5761 aaaggcttct cccatggggg agaagctgcc gcagcaggcg gctgatgagg ggagaacttg
     5821 ccgcaaaagc ccttttacgg ggcggcagcg gcacagccgc ccctcatccc agccaccttc
     5881 ccttgatagg aaggcttttg aggagttccc ctatgtcaaa ctacaacccc tacaccaaac
     5941 gccagacgat gatcgcgccg gattacgagg tctaccgcta ccgctcgacc tacatgaacg
     6001 aggtcgagct gcaccaccat gacttctatg aggtctacct gctgctgcgc ggccgggtgg
     6061 aatatatcgt ggagaaccag ctttaccgcg tgcgccccgg cgactggatg ctgtgcagcc
     6121 cgctggaatt gcaccaggcc cgcattgcca ccgatgccga cgcctacgag cgcattgtgc
     6181 tgtggatcgc gcggccgttt ttggagaacc tgtccacccc gcacaccagc ctgacccgct
     6241 gctttgacac ctcgatcccc agccacacga acctgctgcg gctgcccggg gccaccggtg
     6301 cgccgctgcg caccacggtg gacaagctgt gcgccttaaa ggcaagcaag gactacggca
     6361 gtgacctgtt ggcccagagt gcgctggtgg agctgatggt gggcctgaac cgtgccgctg
     6421 ccgagcgcgg cgacgcccgc cccaccggca ccagtgacca ggtcgtggac gcggtgctgc
     6481 actacatcaa cgagcattac agcgaggccc tgacgctgga tcagctgtcg gaaaagtttt
     6541 tcatcagcaa ataccacctg ctgcgcaagt ttgatgccca ggtaggtacg acggtacacc
     6601 gctatatttt gcaaaagcgc ctgctgaatg ccaagcagct gctggccggc ggcgtgccgc
     6661 cgaatgaggt ctgccagtac tgcggctttg gcgattacgc gaacttctac cgcgcgttcc
     6721 gggccgagta caaccagacg ccgagacagt atatccagag tgccaggggg aagtgatcgg
     6781 ctgaaggggt aacggcagtc ccccggaagg ttgttctttc ttttggcagc aaaagaaaga
     6841 accaaagaaa actgccgcta ttgcgacggg gtgcggggtt gcgaacacgg ggccacagtt
     6901 gagcggagcg aaactggggt agtgagcaat gcttgcccct gcggggcaag caaccgcaag
     6961 agcgggtttg cgcagcaaac tggcggcgct cgttgtgcgc cgccaagcgg gacccgcgac
     7021 caaggggctg cacgcccctt ggattcccaa agcggaggtc ggaagattcg gcggctaaac
     7081 ggacgcagta ttactgtccg ttcttaacgc cgccgaacct tcctccactt taaattcggg
     7141 tgttgcgggg ttgcgagcga ggggccacag ttgagcggag cgaaactggg gtagtgagca
     7201 atgcttgccc ctgcggggca agcaaccgca cgagcgggcc agctttgctg gctggcggcg
     7261 ctcgttgcgc gccgccaagc gccccgaacc cccggtgccg gaaacagcag tccgtagggg
     7321 ccgggcatgc ccggcccgca gccttgcgtc agctcccggt ttcgctttat gcccccttgt
     7381 atgttacatc gtagggaggg gtcttgaccc ctccgctacc ttccgttaaa ctgccgtcaa
     7441 cggttcatcc gtaggggagg aattcatccc tcctgggtac ttcccgccgc cgcaaacggc
     7501 cacggcggcc agagggctgg ccgccctaca accctacttg aaaatccgtg cgt
//
LOCUS       DBMDSQ010000065         7493 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_2628_length_7493_cov_1.774351, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000065 DBMDSQ010000000
VERSION     DBMDSQ010000065.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 7493)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7493)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7493
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2628_length_7493_cov_1.774351"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..582
                     /locus_tag="ACZPAX_03690"
     CDS             <1..582
                     /locus_tag="ACZPAX_03690"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012347.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HIR1113031.1"
                     /translation="MTGIFRSSTADLSTTFTWALMTSFLCWGCSIKRNGVGGWLKGFT
                     EPIVVMTPMNLVSEIAQPISMAFRHFGNIAGGSVLTSLVYSALATVSAAVLGLVGRSL
                     VVGIVILAIGAALLYSGVKGKKMARKIFGTVFAVTGALSLLGLTGVPYLEVGVPGILS
                     LYFDIFSGGVQALVFSLLTMVYVGNACPPPAEA"
     gene            615..854
                     /gene="atpE"
                     /locus_tag="ACZPAX_03695"
     CDS             615..854
                     /gene="atpE"
                     /locus_tag="ACZPAX_03695"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005355792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HIR1113032.1"
                     /translation="MEAAIVKAACAIGAGIAMGFGAIGPAIGEGNAVGKALEGMARQP
                     EMANDLRTNMILGCAITESTGIYSLVIALLLLFVF"
     gene            885..1391
                     /gene="atpF"
                     /locus_tag="ACZPAX_03700"
     CDS             885..1391
                     /gene="atpF"
                     /locus_tag="ACZPAX_03700"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HIR1113033.1"
                     /translation="MTGFESFVGVNPWTALFTFCNMLITFAVLRKFLFKPVKKMIDDR
                     QQEIDTMYADADAAKQKAADLEKEYADHLQRIKDERDTMLREATARAQKREEEIVSEA
                     KAEAASIRAAAEADIAQERKKAVNDLKNEIGGMAVEIAGKVVEREIKESDHQALIDEF
                     IRNVGDAS"
     gene            1388..1924
                     /gene="atpH"
                     /locus_tag="ACZPAX_03705"
     CDS             1388..1924
                     /gene="atpH"
                     /locus_tag="ACZPAX_03705"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005930273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HIR1113034.1"
                     /translation="MTELAKRYGGSLYDLAAEEKLTEELLQELQTAVDSIEAEPQYKR
                     LLATPSVPKKERCALLDKAFEGAHPYLVNFLKLLCEENLIGELPGVLRAYRDRYNEDN
                     GILEVTAVSAVALAAPAREKLLAKLQKMTGKNIVLTETVDPSVLGGLRLDMGGKRLDG
                     TVQRHLERLREEIDGAVL"
     gene            1967..3487
                     /gene="atpA"
                     /locus_tag="ACZPAX_03710"
     CDS             1967..3487
                     /gene="atpA"
                     /locus_tag="ACZPAX_03710"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002847162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HIR1113035.1"
                     /translation="MQLKPEQISRIIRSQIKYYENAIEQTETGTVTMVGDGIARAAGL
                     DNCMAGELVQFESGTYGMAQNLEENSVSIVLLGDDEGIKEGSTIKRTGKVVSVPVGEG
                     MIGRVVNALGQPIDGKGPIEAADYRAIESPAPGILDRQPVKQPLQTGIKAIDSMIPIG
                     RGQRELIIGDRQTGKTVIATDTIINQKGKDVLCIYVAIGQKRSTVATLVENLEKNGAM
                     AYTTVVCATASELSPLQYIAPYAGCAMGEYFMNKGRHVLIIYDDLSKHAVAYRALSLL
                     IRRPPGREAYPGDVFYLHSRLLERAAKLSDAKGGGSLTALPIIETQAGDVSAYIPTNV
                     ISITDGQIFLETELFHAGIRPAVNPGISVSRVGGNAQIKAMKKVAGTLKLIYSQYREL
                     QGFAQFGSDLDADTKARLAQGERIVEVLKQDRNSPVAVEKQVAILYATIHGYLKEVAV
                     SDIAEYEQRLYDYLDENVTAAGVMETIRTTGDLKPETEEQLKQVLADFTAQFLKEK"
     gene            3509..4363
                     /gene="atpG"
                     /locus_tag="ACZPAX_03715"
     CDS             3509..4363
                     /gene="atpG"
                     /locus_tag="ACZPAX_03715"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HIR1113036.1"
                     /translation="MAGSMKEIKLRIRSVESTMQITKAMELVASSKLRRAKERVEGSR
                     PYFETLYETLFDIVGTDSAFDSPYLAKRETNRRLYIVIAGDRGLAGGYNANILKAIEA
                     DAASSDYCVLPIGKKAVEHFERRKANVLTTAFAEAGDLSVSDCFEISRLVCQKFLAGE
                     FDEIRLGFTQFVSMLTQTATVLPVLPFDAKRPEPGHERESLMLYEPDGKAVFDSIIPE
                     YLAGVVYGALCESVASEQGARRMAMDAATKNAGEMIDHLNLYYNRARQAAITQEITEI
                     VAGADAES"
     gene            4394..5788
                     /gene="atpD"
                     /locus_tag="ACZPAX_03720"
     CDS             4394..5788
                     /gene="atpD"
                     /locus_tag="ACZPAX_03720"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HIR1113037.1"
                     /translation="MPEKHIGRVVQVMGPVLDIRFKDGELPALLNAIEVENNDKKLIV
                     EVAQHVGDNVVRCIAMAATDGLVRGADAVDTGEPIKVPVGDECLGRVFNLLGEPVDEQ
                     PAPENTERWPIHRQAPAFDEQESATEILETGIKVVDLICPYAKGGKIGLFGGAGVGKT
                     VLIQELIYNIATAHNGYSVFTGVGERTREGNDLYGEMRESGVLNKTALVYGQMNEPPG
                     ARMRVALSGLTMAEYFRDVKHQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQPTL
                     ATEMGALQERITSTRKGSITSVQAVYVPADDLTDPAPATTFTHLDATTVLSRDIASQG
                     IYPAVDPLDSTSRILSPEVVGQEHYEVARAVQQVLQRYKELQDIIAIMGMDELSEEDK
                     VTVARARKVQRFLSQSFHVAEQFTGMPGQYVPLKETIRGFKMILNGECDSIPESCFLF
                     AGTIDDVLAKAKQQ"
     gene            5801..6214
                     /gene="atpC"
                     /locus_tag="ACZPAX_03725"
     CDS             5801..6214
                     /gene="atpC"
                     /locus_tag="ACZPAX_03725"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HIR1113038.1"
                     /translation="MATFPLKILTPDGVAYDGPAVSVSCRTINGQVQLLAKHIDFCTA
                     LGMGEAHIKLEDGTTRRAACMGGMLSMLKGECRLLATTFEWADAIDAERAARSKTRAE
                     EMLAQKNLDKRDMELAEARLKRALVRSSVAARKDI"
     gene            6333..6593
                     /locus_tag="ACZPAX_03730"
     CDS             6333..6593
                     /locus_tag="ACZPAX_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HIR1113039.1"
                     /translation="MNTTFIYIIIMNIIGFCLMGLDKHRARTRQWRIPEKVLFGAALL
                     GGSAGAWAGMYAFRHKTRRWYFVVGMPLILAVQTGLLWYFLR"
     gene            6816..>7493
                     /locus_tag="ACZPAX_03735"
     CDS             6816..>7493
                     /locus_tag="ACZPAX_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HIR1113040.1"
                     /translation="MSIFENFEKKLQAEPKSIVFTEGTDARILSAASRLLAGKTLKVI
                     LLGEVEAVKKAAADGSFDITGAEIIDPTNYAGFDEMVAKMVELRKGKMTDEQCRAALS
                     KGNYFGTMLVKMGKADCLLGGATYSTADTVRPALQLIKCKPGIKSVSSCFILMRGDES
                     IAMGDCAINIDPSEDEIVESTVETAHTAEVFGIDPRVALLSYSTKGSGKGETVDKMRN
                     ATARVQEA"
BASE COUNT         1599 a         2176 c         2188 g         1530 t
ORIGIN      
        1 atgaccggta ttttccgctc atctaccgct gatttgtcca cgacctttac ctgggcactg
       61 atgaccagct ttctatgctg gggctgttcc atcaagcgca acggcgtggg cggctggctc
      121 aagggcttta ccgagcccat cgtggtcatg acgccgatga accttgtctc tgaaatcgca
      181 cagccgatct ctatggcatt ccgtcacttc ggcaacattg ctggcggcag cgttctgacc
      241 agtctggtct attcggcgct ggcaaccgtc tcggctgccg tgctgggcct tgtgggccgc
      301 agccttgtgg tgggcatcgt gatccttgcc attggtgcgg cgctgctgta cagcggcgtc
      361 aagggcaaaa aaatggcccg taaaatcttt ggcacggtct ttgccgtgac cggcgcactc
      421 tccctcttgg ggctgacagg cgtaccttat ctggaggtcg gtgttcccgg catcctgagc
      481 ctgtattttg atattttctc cggcggcgta caagcccttg ttttcagcct gttgacaatg
      541 gtgtatgtgg gcaatgcctg cccgcccccg gcagaagcat gacattgtat ttattatttt
      601 aggaggattc ttttatggaa gctgcaatcg taaaagctgc ctgcgccatc ggcgccggta
      661 tcgcaatggg ctttggtgct atcggcccgg ctatcggtga aggcaacgcc gtcggcaagg
      721 cgctggaggg catggcccgc cagcctgaaa tggccaacga cctgcgtacc aacatgattc
      781 tgggctgcgc catcaccgag tcgaccggca tttactcgct ggtcatcgcg ctgctgctgc
      841 tcttcgtgtt ctgataaatc ttgctgaaat tggaggaata gctcatgacg ggctttgaaa
      901 gttttgtagg cgtcaacccg tggactgcgc tgtttacctt ctgcaatatg ctcatcacct
      961 ttgcagtgct gagaaagttc cttttcaagc cggtaaagaa gatgatcgac gaccgccagc
     1021 aggagattga tacgatgtac gccgacgccg atgctgccaa gcagaaggcc gccgaccttg
     1081 agaaagagta cgcggatcat ctgcagcgca ttaaggatga gcgcgacacg atgctgcgtg
     1141 aggcgaccgc ccgcgcccag aagcgcgagg aagagatcgt cagcgaagcc aaggccgagg
     1201 ctgcgtccat ccgcgctgcg gcagaggcag acattgccca ggaacgcaag aaggctgtca
     1261 atgacctgaa aaacgagatc ggcggcatgg ctgtcgagat tgccggtaag gtcgtcgagc
     1321 gcgagatcaa ggagtctgac catcaggctt tgatcgatga atttatccgg aatgtggggg
     1381 atgcctcatg accgagcttg cgaaacgcta cggcggaagc ctgtatgacc tggcagccga
     1441 ggaaaaactg accgaggagc ttttgcagga attgcagact gcggtggaca gcatcgaggc
     1501 cgagccgcag tacaagcgcc tgctggcaac gcccagcgta ccgaaaaaag agcgctgcgc
     1561 cctgctggac aaggcgttcg agggggcgca tccgtacctt gtcaactttc tcaaactttt
     1621 gtgcgaggaa aatttgattg gggaactgcc cggcgtgttg cgtgcctacc gggaccgtta
     1681 caatgaggat aacggcattc tggaagttac ggcggtatcg gctgtggcat tggccgctcc
     1741 cgcccgcgaa aaactgctgg caaaattgca gaagatgaca ggcaaaaaca tcgtcctgac
     1801 cgagaccgta gaccccagcg tgctgggcgg cctgcggctg gatatgggcg gcaagcgcct
     1861 ggacggcacg gtgcagcgcc atttggagcg cctgcgcgag gaaatcgacg gtgccgtgct
     1921 ttaacacatt acacctattt tatagaattg tgtggtgaac caaacagtgc aactaaaacc
     1981 cgaacaaatc tcccgcatca tccgttccca gatcaagtac tacgaaaatg ccattgagca
     2041 gaccgagacc ggaacggtaa cgatggtggg cgacggtatt gcgcgcgctg cagggcttga
     2101 caactgtatg gccggtgagc tggtccagtt tgagagcggt acctacggca tggcgcaaaa
     2161 cctggaagaa aacagtgtct ccatcgtcct gcttggtgac gatgaaggca tcaaagaggg
     2221 cagtaccatc aagcgcaccg gtaaagtcgt ttccgtgccg gtaggcgagg ggatgattgg
     2281 ccgtgtcgtc aacgcgctgg gccagcccat cgacggtaaa ggccccatcg aagctgccga
     2341 ctatcgtgcc attgagtcgc ctgcgcccgg aatccttgac cgccagccgg tcaagcagcc
     2401 gctgcagacc ggcatcaagg ccatcgactc gatgatcccc attggccgcg gccagcgtga
     2461 gctgatcatc ggcgaccgcc agaccggcaa gaccgtcatt gcgaccgata ccatcatcaa
     2521 ccagaagggc aaggatgttc tgtgcatcta cgtcgccatc ggtcagaagc gttctaccgt
     2581 ggcaacgctg gtggagaacc tggagaaaaa cggtgctatg gcgtacacaa cggtcgtctg
     2641 cgccaccgcg tccgagctgt cgcccttgca gtatatcgcc ccttacgcag gctgcgccat
     2701 gggcgagtac ttcatgaaca agggcagaca cgtcctgatt atctatgacg atttgtccaa
     2761 gcatgctgtc gcgtaccgtg cgctgtcgct gctgattcgc cgtcccccgg gacgcgaggc
     2821 ataccctggc gacgttttct atctgcacag ccgtctgctg gaacgcgccg ccaagctgag
     2881 cgacgccaag ggcggcggct cgctgaccgc actgcccatc atcgagacac aggctggcga
     2941 cgtttcggcc tatatcccga cgaacgttat ttccattacc gacggtcaga ttttcctgga
     3001 gaccgagctg ttccatgcag gtatccgccc ggctgtcaac cccggtatct cggtttcccg
     3061 tgtcggaggc aacgcccaga tcaaggccat gaaaaaggtt gccggtacac tgaaactgat
     3121 ttactcccag taccgcgagc tgcagggctt cgcccagttc ggctctgacc tggacgccga
     3181 cactaaagcg cgtctggcgc agggcgagcg catcgtcgaa gtgctgaagc aggatcgcaa
     3241 cagtcctgtc gccgtcgaga agcaggttgc tattctctat gcgacgattc acggctatct
     3301 gaaagaggtc gccgtgtccg atattgccga gtatgagcag cgtctctatg actacctgga
     3361 cgaaaatgtc accgctgctg gcgtgatgga gaccatccgc accaccggcg atttgaaacc
     3421 ggagaccgag gaacagctca agcaggtact ggccgacttt actgcacagt tcctcaaaga
     3481 aaaataaact gcaaaggagg tgccgccgat ggccggttcc atgaaagaaa tcaagctgcg
     3541 tatccgaagc gtcgaaagca ccatgcagat caccaaggct atggagctcg tggcgtccag
     3601 caaactgcgc cgcgccaaag agcgtgtcga gggtagccgc ccctattttg agacgctata
     3661 cgagacgctg tttgacatcg tggggacaga ttccgcgttt gacagtcctt accttgccaa
     3721 gcgggagacg aaccgccgcc tgtatatcgt cattgcaggc gaccgcgggc tggcgggcgg
     3781 ctacaacgcc aacatcttaa aggcaatcga agccgacgct gcgagcagcg actactgcgt
     3841 gcttcccatc ggcaaaaagg ccgttgagca tttcgagcgc cgcaaggcca atgtgctgac
     3901 tactgccttt gccgaagcgg gcgatttgtc cgtgagtgat tgctttgaga tttcccgcct
     3961 tgtctgccag aaatttctgg caggggagtt tgacgagata cgccttggtt ttacgcaatt
     4021 tgtctcgatg ctgacccaga cggccaccgt gctgccggtg ctgcctttcg acgccaagcg
     4081 ccccgagccg ggccacgagc gcgagagcct gatgctgtac gagccggacg gcaaagctgt
     4141 ctttgactcc atcatccccg agtatcttgc aggtgtcgtc tacggtgctt tgtgcgagag
     4201 cgttgccagt gaacaaggcg cgcgccgtat ggccatggac gccgccacta agaacgcagg
     4261 tgagatgatc gaccacctga acctgtatta caaccgcgcc cgccaggccg ccatcaccca
     4321 ggaaattacc gagattgtgg ctggcgcgga cgccgagtct taaacgaaaa agcccactaa
     4381 aggagtgtgt gctatgccag aaaaacacat cggaagggtc gtacaggtca tgggacccgt
     4441 actggacatc cgattcaagg acggtgaact gcctgccctg ctcaacgcca ttgaggtgga
     4501 aaacaacgac aaaaagctga tcgtcgaggt cgcccagcat gtcggcgaca acgtggtccg
     4561 ctgcattgcc atggccgcca ccgacggcct tgtgcgcggc gcggatgctg tggacaccgg
     4621 tgagcctatc aaggtgcccg tcggtgacga atgcctcggc cgcgtgttca acctactggg
     4681 tgagccggtc gacgagcagc ccgcccccga aaataccgag cgctggccca tccaccgcca
     4741 ggcccccgct tttgacgagc aggaatccgc aacggaaatt ctggagaccg gcatcaaggt
     4801 cgttgacttg atatgcccct atgccaaggg cggcaaaatc ggtctgttcg gcggtgccgg
     4861 tgtcggcaaa accgttctga tccaggaatt gatctacaac atcgcgacgg cccacaacgg
     4921 ctactctgtc tttaccggcg tcggcgagcg cacccgcgag ggcaacgacc tgtacggtga
     4981 aatgcgcgag agcggcgttc tgaacaagac cgcgctggtc tatggccaga tgaatgagcc
     5041 gccgggagca cgtatgcgcg tcgccctgtc cggcctgacg atggcggagt atttccgcga
     5101 tgtcaagcat caggatgtgc tgctcttcat tgacaacatc ttccgtttca cgcaggccgg
     5161 ttccgaggtc tcggcgctgc tgggccgtat gccctctgcc gtcggctacc agccaacact
     5221 ggccaccgaa atgggtgcct tgcaggagcg tatcacatcc acccgcaagg ggtccatcac
     5281 gtctgtgcag gccgtctacg tgcccgctga cgacctgaca gaccctgcgc ccgccactac
     5341 ctttacccac ctggatgcca ccacggtttt gagccgtgat atcgcctccc agggcattta
     5401 tccggctgtt gacccactgg attctacctc tcgtatcctt tcgcccgagg tagtcggcca
     5461 ggagcattac gaggtcgccc gcgccgtgca gcaggtcttg cagcgctaca aggaattgca
     5521 ggacatcatc gccatcatgg gcatggatga actgtccgag gaggacaagg tcacggtcgc
     5581 ccgcgcccgc aaggtgcagc gcttcctgtc ccagtccttc catgtagcgg agcagttcac
     5641 cggtatgccc ggccagtatg tgccgctgaa ggaaaccatc cgcggcttca agatgattct
     5701 gaacggcgag tgcgacagca tccccgagag ttgtttcctg tttgcaggca ccatcgatga
     5761 tgtgctggct aaggccaagc aacagtaagg tggtgggcgt atggcaactt tcccgctcaa
     5821 aatcttaacg ccggacggtg tcgcctatga cggcccggcc gtgtcggtat cctgccggac
     5881 catcaacggt caggtgcagc tgctggcaaa gcatattgac ttctgcacgg ccctgggtat
     5941 gggcgaggcc cacatcaagc tcgaggacgg caccacccgc cgcgccgcct gcatgggcgg
     6001 tatgctcagt atgctgaaag gcgagtgccg tctgctggcg accacctttg aatgggcgga
     6061 cgccatcgac gccgagcgcg ccgcccgcag taagacccgc gccgaagaaa tgctggccca
     6121 gaagaacctg gacaagcgcg acatggagct ggccgaagcc cgcttgaagc gtgccctggt
     6181 gcgcagcagt gtggccgcac gcaaggatat ttgaatcatt tctgcctccg ctgacaaagc
     6241 ggaggtattt tgtcttttac ggcggtgggt ggcgtcttcg agacgccccg ccagcacttg
     6301 ccttttgccc ccacacacag tataatagac gcatgaatac tacatttatt tacatcataa
     6361 tcatgaacat catcggtttc tgcctgatgg ggctggacaa gcaccgcgcc cgcacgcggc
     6421 agtggcgcat cccagaaaaa gtcctgttcg gcgcggcact gctgggcggc agcgccgggg
     6481 cctgggcagg gatgtacgcc ttccgccaca agacgcgccg ctggtatttt gtcgtgggca
     6541 tgccgctgat tttggctgtg cagaccgggc ttctgtggta ctttttgcgc tgaacacaca
     6601 gctaagcggg tgaatttgac aaataatcta caatctcagc ttgacatttt attaactttg
     6661 tgaaatattt tgcctcgaac gccttttccg cacatatgtt gcgcaaattc ggcgcatttc
     6721 gcacattttt attgcaaagc gcaaaatggg tgctataata accatgtaaa tgctgcgcag
     6781 cccttggctg cataaatact ggaggttatc agagtatgtc cattttcgag aattttgaga
     6841 agaaactgca ggctgagccg aagtccatcg tctttaccga gggcaccgac gcccgtatcc
     6901 tgtctgctgc aagccgcctg ctggccggca agaccctgaa ggtcatcctg ctgggcgagg
     6961 tcgaagccgt caaaaaggcc gctgctgatg gcagctttga tatcaccggt gccgagatca
     7021 tcgaccccac caactacgcg ggctttgacg agatggtcgc caagatggtc gagctgcgca
     7081 agggcaagat gaccgacgag cagtgccgcg ctgctctgtc caagggcaac tacttcggca
     7141 ccatgctggt caagatgggc aaagctgact gcctgctggg cggtgccacc tattctaccg
     7201 ccgataccgt ccgccccgcg ctgcagctca tcaagtgcaa gcctggcatc aagtccgtca
     7261 gctcctgctt catcctgatg cgcggcgacg agagcattgc catgggcgac tgcgccatca
     7321 acatcgatcc ttctgaggac gagatcgttg agtccactgt tgagaccgca cacaccgctg
     7381 aggtcttcgg cattgatccc cgcgttgctc tgctgtccta ctccaccaag ggttccggca
     7441 agggcgagac cgtcgacaag atgcgcaatg ctaccgcccg cgtccaggag gca
//
LOCUS       DBMDSQ010000066         7486 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_2633_length_7486_cov_1.669871, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000066 DBMDSQ010000000
VERSION     DBMDSQ010000066.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 7486)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7486)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7486
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2633_length_7486_cov_1.669871"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            20..454
                     /locus_tag="ACZPAX_03740"
     CDS             20..454
                     /locus_tag="ACZPAX_03740"
                     /inference="COORDINATES: protein motif:HMM:NF045650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD1247 N-terminal domain-containing protein"
                     /protein_id="HIR1113041.1"
                     /translation="MDLNAKAAYIRGLMTGMEFDADSKNGKVIAAMMDLLEEMAAQVT
                     EHDDALDQVYDELETLDQDVDDIVSDLYADDDDEDEDDEDDDSTMYEVTCPNCGQIST
                     LDEETLMDPEGDLVCPHCGATFDVELEGTEEDSAEETDKQDQ"
     gene            526..1770
                     /locus_tag="ACZPAX_03745"
     CDS             526..1770
                     /locus_tag="ACZPAX_03745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113042.1"
                     /translation="MPEPVKTASSLAAALLFLWSAVWCIRRVFSPEPAPHYTEPSSRS
                     RCWAAIAGAAFILVLHLIFAVTAHINYPEADFRQAIEWQFYGNTDSRHYLDLSIYGYG
                     TQEAFPEQYLQIVFFPLYPALLHILHFVTRIDAFWLGFAVQLPLFAAATASFYTVIAR
                     RWGEQTAALSLALLLASPASVFFAVPMTESLFLLLTVRYVLTLEREQWWRCASLGVLA
                     GLCRAPGGLLLGLTGLYLFMRWRRGTRPTIPSLLAMLSPAAGLGLYFLLNYLVYGRWN
                     QYQIYQWDNWNQKLGLFTNTVRYHLDYMAQWWDSSPKSALALCLTAVLCLLTILALLS
                     ATVRRLPAHWLGFGLAYFAVTMGATWLISAPRYAVGLFCLPIALALLLQGRPRLTKGV
                     LVTQVIVSVIYTMQFLYGWPIY"
     gene            complement(1827..2114)
                     /locus_tag="ACZPAX_03750"
     CDS             complement(1827..2114)
                     /locus_tag="ACZPAX_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113043.1"
                     /translation="MAYTPKLTYKGRPLVRCGNDIYYGSMKDPFVVYLQVLSTKKENG
                     VDVADKVHIVLLSTDETKPLPERIARQANKVGLYNALSFGDIMLQGALQKK"
     gene            complement(2216..2575)
                     /locus_tag="ACZPAX_03755"
     CDS             complement(2216..2575)
                     /locus_tag="ACZPAX_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2304 domain-containing protein"
                     /protein_id="HIR1113044.1"
                     /translation="MFDQTLIRICLILGSFITAGYILRRVRQAKVQIEDTIFWLLFSA
                     ALLILAIFPGIAYWAANLLGFMSPINFVYVVIIFLLLAKQFFMSIKLSQLDSKVRILT
                     EQVALNEEKVERDKLDL"
     gene            complement(2577..3296)
                     /locus_tag="ACZPAX_03760"
     CDS             complement(2577..3296)
                     /locus_tag="ACZPAX_03760"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR1113045.1"
                     /translation="MSKLLIVIPAHNEEANIVQVVEELTRDYPQYDYVVVNDGSRDKT
                     AAVCRAHGYRLIDLPVNLGLAGAFQTGLRFAAENDYDCAMQMDADGQHKPEYIEAMMK
                     ELEAGSDIVIGSRFLTVKKPKTLRMLGSYIISWSIRLTTGRAICDPTSGMRMFNRAMV
                     EEFAQNLNYGPEPDTISYLIKNGATVKEVQVEMGERMAGQSYLTFWRSVQYMVKMSIS
                     ILLIQWFRKRDTETPYPERSL"
     gene            complement(3298..4428)
                     /locus_tag="ACZPAX_03765"
     CDS             complement(3298..4428)
                     /locus_tag="ACZPAX_03765"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR1113046.1"
                     /translation="MVICFFSTQYLPTPGGVERYIWNLARRCVAAGHRALVVAASLPG
                     LPARETGPDGIEIFRLPSYPVMGGRFPVLHPFVDAGDLWAQGIDFAVIQTRMYPQSVW
                     AARQCKRRGIPALVIDHSTGYMMHGGLAGALGKAYEHAACGCIRRCGFPFYGVSGDVC
                     RWLQTFGIAAAGRLPNAVDPDELDKLARAENAVDWRTKLNVPAGGKLIAFVGRLIPEK
                     GALRLANAVQAIPGCVLAVAGTGPEEPSLAALGGPIHVLGALPHEQIVQLLAQADAYC
                     LPTEYAEGFPTTLLEAAACRCPIVTTRTAGTGELLPGGDYAVFLADASTQTIRAGLDT
                     LMADPAAAAARAEKAHENLCSHFTWQAVFATMMEIVNAAKRS"
     gene            complement(4442..7018)
                     /locus_tag="ACZPAX_03770"
     CDS             complement(4442..7018)
                     /locus_tag="ACZPAX_03770"
                     /inference="COORDINATES: protein motif:HMM:NF021436.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2142 domain-containing protein"
                     /protein_id="HIR1113047.1"
                     /translation="MKQQWKNHKPLLAAWATLVVLLAGVVLIWLAQVRPNVGKGRSEI
                     ITDFLRAELIHDGETAEQVFTYDKDILTIGLEFYLPGAQPSGELDVVLYDADTGEELA
                     RSVGTMDYIVPDQYTTLGLDPAVTGQEGRCYRLTVTPHYTTDAILAVGHSDGVALWKE
                     QMSVADRAVDGTMAMQITYQRIGGYLTRFFLVVGGFAALLAAFAVWAVLSKKLALHRL
                     VFVLVLGFGLLYSFVLPPYAAPDEKYHINQSFTLACRWANDLSRDEWRMGKVPTTTSF
                     RRVGDEDADLQDENTTVFTWEEFTSELFTTTDQPFDSHQEYNELQTDQNPLLYVVSAA
                     AVFLAYVLHLGFAPALFLGRLANLLLFAALAALAVKAAPFGKRVFTVAALLPMTLHLA
                     ASFSRDAPLLGLCFAFTALLLDAAFGPEKQKALSPARLALLLGCGVLLAPGKLVYLPL
                     AALLLVVPAARLGRHAKAKKCGYLAVCLALALLLNTGLLADTLHSGAGEQTETSETTE
                     TTAEATNIEDTDRTPKGRPEERDADYEVEICTVNTTENFLRRLYYFIDNTRDPAQSEL
                     DFWVQALNDGDVTPAVLEQSFFLSPEHINEYNDISDFYWNVCSALLGTNVSYGNEDAF
                     YPYFEQGGPVLAYKQLLGLDGTQTYFAELGLEVGSMDDRIPLDRAELAKEVEAARATR
                     STQSTADEADKTTYTPGYILRHPVATVMLFVRSAVENGDHYIRTLVGGSLSYYTVDLA
                     WGWVVVLYLLLAYAALPVQGAALAPCGKARGWCCAATVVSCLLAVAGCLLWTPTHYET
                     LYGLQGRYFLPVLPLLLLTCLPRRLAAVPDEDTAQSHLTLALALVQAGVVVNIMLAVI
                     AR"
     gene            complement(7031..>7486)
                     /locus_tag="ACZPAX_03775"
     CDS             complement(7031..>7486)
                     /locus_tag="ACZPAX_03775"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KdsC family phosphatase"
                     /protein_id="HIR1113048.1"
                     /translation="VYYDATGNETKKFAIKDGAGLVLARTAGIRVMICTGRECEAVRR
                     RAADLKITDVYQNVGKKAAFLRGFMAENHYAREEVAYCGDDLNDLAAMALCGFVACPA
                     DAAAEVKARADYICPQRGGEGAVRGAVEKILRGDGRWKDAVKKAFDVEL"
BASE COUNT         1422 a         2313 c         2249 g         1502 t
ORIGIN      
        1 ggaggattga attatggcta tggatctgaa tgcaaaggcc gcctacatcc gcggcctgat
       61 gaccggtatg gaatttgacg cagactccaa gaacggcaaa gtcattgccg ccatgatgga
      121 cctgctggaa gagatggccg cccaggtcac cgagcatgac gacgccctgg atcaggtcta
      181 cgacgaactg gagacgctgg atcaggatgt tgacgatatc gtcagcgacc tctatgccga
      241 tgacgacgac gaggatgagg acgacgaaga cgatgacagc accatgtacg aggtgacctg
      301 cccgaactgc ggccagatct ccaccctgga cgaggagacc ctgatggatc ccgagggtga
      361 cctggtctgc ccccactgcg gcgcgacctt cgacgttgag ctggaaggca ccgaggagga
      421 ctccgccgag gagaccgaca agcaggatca gtaattttta aagtatgcca aaaggctcgg
      481 tggataagca ccgagccttt tatgctgaaa tcaggagaat ttcaaatgcc tgaaccagtc
      541 aaaacagcct catccctggc agccgctttg ctgtttctct ggtctgccgt gtggtgcata
      601 aggcgcgtct tttcaccgga gcccgccccg cactatacgg agccttcgtc gcgcagcaga
      661 tgctgggccg ccattgccgg ggccgccttt atccttgtcc ttcatctgat ttttgccgtc
      721 acggcccata taaactatcc cgaggctgat tttcgccagg cgatagaatg gcagttttac
      781 ggcaataccg attctcggca ctaccttgac ctttccatct atggctacgg cacgcaggag
      841 gctttcccgg agcagtatct ccaaatcgtg tttttccctc tgtatcctgc gctgctgcac
      901 attctccact tcgtgacccg catcgacgct ttctggctgg gttttgcagt gcagttgccg
      961 ctctttgcag cggccacagc atccttctac acagtaatcg cccgccgttg gggcgagcag
     1021 acagccgcat tgtcgctggc gcttctattg gccagcccgg cgtccgtgtt ttttgcagtg
     1081 cccatgacag agagcttgtt cctgctgctt acggtgcggt atgtgctgac gctggaacgt
     1141 gaacaatggt ggcgctgtgc ctcgctaggg gttctcgccg gtctgtgccg tgcccccggc
     1201 ggtctgcttt tggggctgac cggcctgtat ctctttatgc ggtggcggcg cggtacgcgg
     1261 cccacaatac cgtccttgct tgctatgttg tccccggctg ccggacttgg cctttatttt
     1321 ctgctgaact acctcgtata tgggcggtgg aaccagtacc agatatacca atgggacaac
     1381 tggaaccaga agctggggct gttcaccaat actgtgcgct accacctgga ctacatggcg
     1441 cagtggtggg acagcagccc caaaagcgcc cttgcgctat gcctgaccgc ggtgctctgc
     1501 ctgttgacaa ttttggcact gctgtctgcc acggtgcgcc gccttccggc gcactggctc
     1561 ggcttcggtc ttgcctattt tgccgtaacg atgggtgcca cctggctgat cagcgccccg
     1621 cggtacgcgg tggggctgtt ctgtctgccc attgccttgg cgctgctttt gcagggccgc
     1681 ccacggctca caaagggtgt attggtaacg caggttatcg taagcgttat ctacacaatg
     1741 caatttctat acggctggcc gatctattaa aaaaactgcc tgtctcctaa atgcggagac
     1801 aggcagtttt ttgattcagc catttcttac tttttctgca atgcgccctg cagcatgata
     1861 tcgccgaagg agagcgcgtt gtacaggccc accttgttgg cctggcgggc aatgcgctcg
     1921 ggcaggggct tcgtctcatc ggtggagagc aggacgatgt gcaccttatc ggctacgtcg
     1981 acgccgtttt ccttcttggt ggagagcacc tgcagataaa cgacgaacgg atctttcatg
     2041 gagccgtagt agatatcgtt gccgcagcgc accagcgggc ggcccttata ggtcagtttc
     2101 ggggtatatg ccatatctga aaccttcttt ctgtttagtc aatactatat tatagcatag
     2161 attttgccgg aatacaaatt tttattgcaa atacctttcc ccctggggga agcctttaca
     2221 ggtccagctt gtcccgctcc actttttcct cgttcagcgc gacctgctcg gtcaggatgc
     2281 gcaccttgct gtccagctgg ctcaatttga tggacatgaa aaactgctta gccagcagca
     2341 ggaagatgat gacgacgtag acgaagttga tggggctcat gaagcccagc aggttggcgg
     2401 cccagtaggc aatgcctggg aagatggcca gaatcagcag cgccgcactg aacagcagcc
     2461 agaagatcgt atcctcgatc tggacttttg cctgccgcac gcgccgcagg atataccccg
     2521 ccgtgatgaa gctgcccagg atcaggcaga tgcgaatgag cgtttgatcg aacatttcac
     2581 aggctccttt cggggtaggg ggtctcggtg tcccgcttgc ggaaccactg gatcagcaaa
     2641 atcgagatgg acattttgac catatactgc acactacgcc agaaggtcag gtagctctgg
     2701 cccgccatgc gttcgcccat ttccacctgc acttccttga cggtcgcgcc gtttttgatc
     2761 aggtagctga tggtgtccgg ttcggggccg tagttgaggt tctgggcaaa ttcctcgacc
     2821 atagcgcggt tgaacatccg catcccgctc gtcgggtcgc agatggcgcg gcccgtcgtc
     2881 agccggatgg accagctgat gatgtagctg cccagcatcc gcagcgtctt gggctttttc
     2941 acggtcaaaa agcggctgcc aatgacgata tcgctgcctg cttccagttc tttcatcata
     3001 gcttcgatat attccggctt atgctggccg tcggcgtcca tctgcatggc gcagtcgtag
     3061 tcattttcgg cggcaaagcg cagcccggtc tggaacgcgc ccgccagccc caggttgacc
     3121 ggcagatcga tgagccggta gccgtgggcg cggcagacgg cggcggtctt gtcgcggctg
     3181 ccatcgttga caaccacgta atcatactgg ggatagtcgc gggtcagttc ctccacgacc
     3241 tgcacgatgt tggcttcctc gttatgggcg gggatgacga tgagtagttt tgacatctta
     3301 gctccttttg gctgcattta caatttccat cattgtagca aacacagcct gccatgtaaa
     3361 gtggctgcaa aggttttcgt gggctttttc ggcccgggcg gccgccgcag caggatccgc
     3421 catcagggta tccagcccgg cgcggatggt ttgggtggat gcgtcagcca gaaagactgc
     3481 gtaatcgccc ccgggcagca gctccccggt gcccgccgtg cgggtcgtca cgatggggca
     3541 gcggcaggcc gcagcctcca aaagggtggt agggaagccc tcggcgtatt ccgtcggcag
     3601 gcagtaggcg tccgcctggg ccagcagctg gacgatttgc tcatgcggca gcgcacccag
     3661 cacgtggatg gggccgccca gcgcggcgag agacggttcc tccgggccgg tgccagccac
     3721 ggccagcaca caaccgggga tggcctgcac ggcgttggca aggcgcagtg cgcctttttc
     3781 ggggatgagt cgcccgacaa aggcgatgag cttgccgccc gcgggcacat tcagcttggt
     3841 gcgccagtct acggcgttct cggcgcgggc cagtttgtcc agctcatccg gatcaacagc
     3901 gttgggcagg cggcccgccg ctgcgatgcc gaaggtctgc agccagcggc agacgtcccc
     3961 cgaaacgccg tagaagggga atccacagcg gcggatacag ccgcaggcgg cgtgctcata
     4021 ggctttgccc agcgccccgg ccagcccgcc gtgcatcata tagccggtag agtggtcgat
     4081 gaccagcgcc gggatgcccc gccgcttgca ctggcgggct gcccagacgc tttgggggta
     4141 catccgggtc tggatgaccg caaagtcgat gccctgcgcc cacaggtcac cagcgtcaac
     4201 gaagggatgc agcaccggga accgcccgcc catgacaggg tacgacggca ggcgaaaaat
     4261 ttcgataccg tcggggccgg tctcccgcgc gggcaggccc ggcagcgacg ccgcgaccac
     4321 gagcgcccgg tgccccgccg cgacgcaccg ccgcgccaga ttccagatat accgctcgac
     4381 cccgccggga gtggggaggt attgggtgga gaagaagcaa ataaccatag tgtaagcctt
     4441 tttatcgtgc aatcactgcc agcatgatat tgaccacaac gcccgcctgc accagcgcca
     4501 gggccagagt caggtgggac tgcgccgtat cctcgtcggg cacggcggcc aggcggcggg
     4561 gcaggcaggt caggagcagc agcggcagaa ccggcaggaa ataccgcccc tgcagaccgt
     4621 acagtgtctc gtaatgggtg ggcgtccaca gcaggcagcc agccacagcc agcaggcagc
     4681 tgaccactgt cgccgcgcag caccagccgc gggctttgcc acagggggcc agcgccgcgc
     4741 cctgcaccgg cagggccgca taggccagca gcaggtacag caccacgacc cagccccagg
     4801 ccagatccac ggtgtagtag cttaaactgc cgcccaccag cgtgcggata tagtggtcgc
     4861 cgttttccac ggcgctgcgc acaaacagca tgacagtggc cacgggatgc cgcaggatgt
     4921 acccgggcgt ataggtagtc ttgtcggcct catccgccgt gctctgggta ctgcgggtcg
     4981 cgcgagcggc ctcgacctct tttgccagtt cggcgcggtc cagcgggatg cggtcatcca
     5041 ttgagccgac ttccagcccc agctcggcaa agtaagtctg ggtgccgtcc agccccagca
     5101 gctgcttata ggccagcacc gggccgccct gttcaaagta ggggtagaag gcatcctcgt
     5161 tgccatagct gacattggtg cccagtaggg cgctgcacac gttccaataa aagtcggaga
     5221 tatcattgta ttcattgata tgctccggcg aaaggaagaa gctctgctcc agcacagcag
     5281 gggtaacatc accgtcattg agagcctgca cccagaagtc cagttcgctt tgggcgggat
     5341 cccgcgtatt atcaataaaa tagtacagcc gacgcaggaa gttttccgtt gtgtttaccg
     5401 tacagatctc tacctcgtaa tctgcgtcgc gttcctccgg gcggcccttt ggcgtgcggt
     5461 cggtgtcttc aatatttgtt gcctcggcgg tcgtctctgt tgtctcgctc gtctccgtct
     5521 gctctcctgc gccgctgtgc agcgtgtcgg ccagcaggcc ggtgttcagc agcagggcca
     5581 gcgccaggca gacggccagg tagccgcact tttttgcttt ggcgtggcgg cccagccggg
     5641 ctgcgggcac caccagcaac agcgcggcca gcggcaggta aacaagcttg ccgggggcca
     5701 gcagcacacc acaacccagc agcagcgcca gccgggcagg ggagagagcc ttctgctttt
     5761 cggggccgaa ggccgcatcc agcagcagcg ccgtaaaggc gaagcacagc cccagcagcg
     5821 gggcatcgcg gctgaagctg gccgccaggt gcagcgtcat gggcagcagc gccgccacgg
     5881 tgaacacccg cttgccaaac ggcgcggcct tcacggccag cgccgccagc gccgcaaaca
     5941 gcagcaggtt tgccagccgc cccaggaaca gcgccggggc aaaccccagg tgcagcacat
     6001 acgccaggaa caccgccgcc gcactgacca catacaaaag cgggttctgg tcggtctgca
     6061 gttcgttgta ctcctggtgg ctgtcaaatg gctggtccgt cgtcgtgaac agctcggagg
     6121 taaactcctc ccaggtaaag accgtcgtgt tctcgtcctg caaatcggcg tcctcgtcgc
     6181 ctacgcggcg gaaggaggtc gtcgtgggca ctttgcccat ccgccattca tcgcgggaca
     6241 aatcgttggc ccagcggcag gcgagggtga agctctggtt gatgtggtat ttttcgtccg
     6301 gcgcggcgta gggaggcagc acaaagctgt acagcagccc aaagcccagc accagaacaa
     6361 atacaagacg gtgcagcgcc agttttttgc tcagcactgc ccacacggca aatgccgcca
     6421 gcagcgccgc aaagccgccc acgaccagga agaaccgggt caggtagccg ccgatgcgct
     6481 ggtaggtgat ctgcatggcc atcgtgccgt ccacggcgcg gtcagccacc gacatctgct
     6541 ctttccaaag cgccacgccg tcgctgtggc caacggccag aatcgcgtcg gtcgtgtagt
     6601 ggggcgtcac ggtcaaccgg tagcagcggc cctcctgccc ggtcacggcg gggtccagac
     6661 ccagcgtcgt gtactggtca ggcacgatat agtccatcgt ccccaccgag cgggccaact
     6721 cctcgccggt gtcggcatca tagagcacca catccagctc gccgctgggc tgtgcgcccg
     6781 gcaggtaaaa ttcaaggcct atcgtcaaaa tatccttgtc gtaggtaaac acctgctcgg
     6841 cggtctcgcc gtcatggatc agctccgcac gcaggaaatc ggtgatgatc tcgctgcggc
     6901 ccttgcccac gttggggcgc acctgcgcca gccagatgag caccacgccc gccagcagca
     6961 cgaccagcgt cgcccaagcg gccagcaggg gtttatggtt tttccattgt tgtttcatgg
     7021 gttctcccaa ttatagctca acatcaaacg cctttttcac ggcgtccttc catcgtccgt
     7081 ccccgcgcag aatcttctcc acggcaccgc gcacggcacc ctcgccgccg cgctgggggc
     7141 agatgtaatc ggcgcgggct ttcacctccg cagcggcgtc cgccgggcag gccacaaaac
     7201 cgcacagcgc catggccgcc aggtcattca aatcgtcgcc gcagtaggcg acttcttccc
     7261 gcgcgtagtg gttctcggcc ataaaaccgc gcaggaacgc cgcctttttg cctacgtttt
     7321 ggtatacgtc ggtgattttc aaatccgcag cacggcggcg cacagcctca cactcgcgcc
     7381 cggtgcagat catcacccgg atgcccgccg tgcgggccag caccagcccg gcgccgtctt
     7441 tgatggcgaa cttcttcgtc tcgttgccgg tggcgtcgta gtagac
//
LOCUS       DBMDSQ010000067         7389 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_2670_length_7389_cov_1.500274, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000067 DBMDSQ010000000
VERSION     DBMDSQ010000067.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 7389)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7389)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7389
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2670_length_7389_cov_1.500274"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            263..1015
                     /gene="recO"
                     /locus_tag="ACZPAX_03780"
     CDS             263..1015
                     /gene="recO"
                     /locus_tag="ACZPAX_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HIR1113049.1"
                     /translation="MQIATTGLVLRQVKVGEADRILTILTPDLGVISASARGSLRMKS
                     PLFSATGLFCYSEFSLSSGRSHFFVDAAQIKKVFHGVSATVEGMALASYMAEIAMELS
                     PTPAEGEADAQLRLLLNCLYLIGERRYPWRQLKAVYELRALSKAGYMPDLRMCAGCGK
                     YEGGEFYLDAVGGLLLCADCAERQRHIPNLDAGALYAMRHIFWTVEDKKVFGFKISPE
                     SLKDLSRVSEQYTLANLEHGLKTLDFLKTVLE"
     gene            1093..1706
                     /locus_tag="ACZPAX_03785"
                     /pseudo
     CDS             1093..1706
                     /locus_tag="ACZPAX_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046313.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     assembly_gap    1707..1716
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1717..3406
                     /locus_tag="ACZPAX_03790"
     CDS             <1717..3406
                     /locus_tag="ACZPAX_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0045910 - negative regulation of DNA
                     recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HIR1113050.1"
                     /translation="EANARIMQLRAQEQEEITRILTSFSTQVGSLEPQFTYSYDAMLK
                     IDLLLAKARLAVEQNAFMPAVSDKVYFKLNKARHPLIDKKKVVPVDIELGSDYDTLVI
                     TGPNTGGKTVSLKTAGLLNAMAQYGFLIPAHESSIVCHFEEYLVDIGDEQSIEQSLST
                     FSGHMKRISGILDLAGHATLTLLDELGAGTDPAEGAALAVSILERLRRQGSLLMATTH
                     YAELKVYALETPGVVNASCEFNVETLMPTYKLSVGVPGKSNAFLISSKLGIPEEIIDA
                     ARNHMSNDDKRLDSVLSQLDDLKVQLKEAQAEAEQAKYDAEHALESAEKKRDALIKKG
                     EEELENARRQAHDLMQQVQNEAYSLTDELRRIQKDEKTSAAQRAVRAREIARKDTETL
                     LKKTDAKPKPVKEFVPLKEVQIGQEVVIADLGQTATVTARPDRNNMVEVRAGIMKTKV
                     PLSNLRAPDKMEKRKPAEPHRSTRVQLDKSRKASMEINLLGYTVDEALNEVDKFLDSG
                     MLRGQTTLYIIHGNGTGALRTAIQKHLRTHKAVKSFRPGRYGEGENGVTVVELK"
     gene            3522..4463
                     /locus_tag="ACZPAX_03795"
     CDS             3522..4463
                     /locus_tag="ACZPAX_03795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113051.1"
                     /translation="MAMDYNSTRSFTMTLAHRAVGDIRRGGFRQLRNYVDMCATLAKK
                     PQQKDFFAYAQKALQRTDSCYYSMIHRLLDSVEEDRICTVGVNMGFGGLIYGASEMKK
                     QADVDGKPIAWITAARCGDERLADMVPKAVKKGSFVWLLDATANDPVQAVSLAKANPQ
                     SAFGLLADPAALDDACVNALTACRNLVVMPLLQTPELTAEACRAARRLKAQKMFYALT
                     VLVDDDTVDEVTQDDWLESIAQETLFCMCARKPGISAESSHKLRRSIVDGRMETGKPV
                     LLLDWDGDVKYLNNRISEHMTFGSVLAEGETFPLQLG"
     gene            complement(4532..5092)
                     /locus_tag="ACZPAX_03800"
     CDS             complement(4532..5092)
                     /locus_tag="ACZPAX_03800"
                     /inference="COORDINATES: protein motif:HMM:NF025636.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HIR1113052.1"
                     /translation="MPRSNQTKHLLAQSLQELMATTPLEKISVNDIVDHAGVGRNTFY
                     YHFEDKYDLVNWYFQSGITQFLVERSAYSSWDSLLTALEAYFLENKVFYCNALAYNGQ
                     NSLQQYMFDYLCSIFAQNARELKVDIPAEEAAKIGQFFSGAMMGVLIPWVFGGMKSNT
                     LAAVSDLAIPLFNHEIMQSLLRGETQ"
     gene            5361..6785
                     /locus_tag="ACZPAX_03805"
     CDS             5361..6785
                     /locus_tag="ACZPAX_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013977472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HIR1113053.1"
                     /translation="MPMNKVVHAAQRAAFSAAIDAAINAVRGKGPENMAAQAVKLVNL
                     AQPLLAHRYPDADWDKVRSFVSDPNSKWMEYAYKAINEIDPHILKMNTLNLVYEGMFA
                     GYNYVMELRKKYDCNMPWILLFDPTSACNLHCKGCWAAEYGNRLNLSFEDMDRIVTEG
                     EELGIHWYMCTGGEPTCRKEDLFKLAAKHQNSVFHLFTNGTLIDQAFCDRVKEVGNMA
                     FFISIEGIGDATDDRRGEGVYDRVMHAMDLMKENGLLFGTSICYTSANYKAVTSDEFM
                     DMLISHGVRFNWYFHYMPIGDGANVDLMLNAEQREYMIHRVREIRGYTGGKPIFCIDF
                     QNDGEYIDGCIAGGRQYAHINPAGDVEPCVFIHYSNANIHDKSLLECLQQPLFKEYHK
                     GQPFNHNHLRPCPMLENPELLGEMVKRSGAHSTDMQQPESTDDVFRRCRPYADQWTPS
                     ADRIWAEEHPDCASCASCSACASK"
     gene            6978..>7389
                     /locus_tag="ACZPAX_03810"
     CDS             6978..>7389
                     /locus_tag="ACZPAX_03810"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF015384.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E processing peptidase SpoIIGA"
                     /protein_id="HIR1113054.1"
                     /translation="MYLDVLLLTNFLMAYFLLLAAGALSGQRGGFVRMLAGSGLAAMS
                     SLILFAPPQPYLVQVVYKITTALAITAAAFGWRSKRRLVTAACWYAALNIALAGAVLL
                     AVLRTGTRAADTANLTVYLRVSPLLLLALSGVCCG"
BASE COUNT         1635 a         2205 c         2120 g         1419 t
ORIGIN      
        1 ctgcagtgct gggtcaaggt ccgcgaggat tgacgcgaca acgaattgca gctgaactcg
       61 ctggggttcg ccaagccgta acggatattt gcccatgccc tttattccgc caacatcccc
      121 gccgcgcctg tgctatcctg ttgccatggg ggtgacgaca tggacaacgc ggcggacggc
      181 tggcagcgat ttgcgcagac cggcagcatt tatgactacc taacgtataa aacacagcag
      241 aacgcagcac gggaggacgt atatgcaaat tgcaacaacg ggtctggtgc tgcgccaggt
      301 caaggtgggg gaggccgacc ggatcctgac gatcctgacg cctgacctgg gggtcatatc
      361 ggcctcggcc cggggcagtc tgcggatgaa aagcccgctg ttcagtgcca cgggcctgtt
      421 ctgctactct gaattttccc tgtccagcgg ccgcagccac ttttttgtgg acgcggcgca
      481 aatcaaaaaa gtgtttcatg gcgtatctgc cacggtcgag ggcatggccc tggccagcta
      541 tatggcggag atcgccatgg agctttcccc tacaccggcg gaaggtgagg ccgacgccca
      601 gctgcgtctg ctgctgaact gcctgtacct gatcggtgag cgccgctacc catggcggca
      661 gctgaaagcc gtctacgaac tgcgtgctct gtccaaagcg ggctatatgc cggatctgcg
      721 tatgtgcgcg ggctgcggca aatacgaagg gggagagttc tacctggacg cggtgggcgg
      781 gctgctgctc tgcgctgact gtgccgagcg gcagcgccat atcccgaacc tggacgcagg
      841 ggccttgtat gccatgcgcc atatcttctg gacggtcgag gataaaaaag tattcggctt
      901 caagatttca cccgagagcc tgaaagatct ttcccgcgtc agcgagcagt acacgctggc
      961 gaacctggaa catgggctga aaacgctgga ctttttaaaa actgtacttg agtaaggaga
     1021 ccaaaaccct gtatggatac cgcctataaa acaacactgg aactggacaa ggtccttgcc
     1081 cgggctgtgc agctgtgcgc ctgccaggaa accaaggaga tgatgcaggc cctggaaccc
     1141 gcccccacca ttgaggacga gcgctacgac ctggctcaga ccaacgccat caacgcgctg
     1201 ctcatcaaaa acggcagccc ccgcttcggc agcgtgcgcg aggtgcgccg catcgtcgcc
     1261 cacgcccgca agggcggcat cctctcgatg ggcgaactgc tggaaattgc cgcgacgctg
     1321 cgcaactttt cgggcctgag ccagtggtac ggcctgagcg agcacgaaat gcttcccacc
     1381 gacgatttgt ttttctcgct ggccccgcag ccggtgctgg aaaaacagat cagcgagagc
     1441 attctttccc cagaggaaat ggccgatacc gccagcgtaa cgctgcacga tctgcgccgc
     1501 aaaatccgcc agaccgagga ttccatccgc accaaactcg ataacatcat ccgcaactcc
     1561 accaccaaca aattcctgca ggatgccgtt gtttcgctgc gcaatggccg ctatgttgtg
     1621 ccggtccgcg ccgagtaccg cggtgaggtg ggcggcgtta tccatgacgt ttcctctacc
     1681 ggcgcgacgg tgttcgttga gccgacnnnn nnnnnncgag gccaatgccc gcattatgca
     1741 gctgcgcgcc caggagcagg aggaaatcac ccgtatcctg acgtccttct ccacacaggt
     1801 cggctcactg gagccacagt tcacgtatag ctacgatgct atgctgaaaa tcgacctgct
     1861 gctggcaaaa gcacggctgg ccgtcgagca gaacgccttt atgcccgctg tcagcgacaa
     1921 ggtctatttc aagctgaaca aggcccgcca cccgctcatc gacaagaaaa aagtcgtgcc
     1981 ggtggatatc gagctgggca gtgactacga cacgcttgtc atcaccggcc ccaacaccgg
     2041 cggcaagacg gtgtccttaa aaaccgcagg cctgctcaac gccatggccc agtacggttt
     2101 tctgattccc gcccatgaga gcagcatcgt ctgccacttt gaggaatacc tcgtggatat
     2161 cggcgacgaa cagagcatcg agcagagcct gtccaccttc tccggccaca tgaagcgcat
     2221 cagcggcatt ctggatctgg caggccacgc taccctgacc ctgctggacg agctgggtgc
     2281 gggcaccgac cccgccgagg gcgcagcgct ggctgtcagc attctggaac ggctgcgccg
     2341 ccagggcagc ttgctgatgg cgacgaccca ctacgcagaa ctgaaagtct acgcgctgga
     2401 aactcccggc gtcgtcaatg ccagctgcga atttaacgtg gagacgctga tgcccaccta
     2461 taaactcagt gtcggcgtgc cgggcaaatc caacgcgttc ctcatcagct ccaagctcgg
     2521 catcccggaa gaaatcattg atgccgcgcg caatcatatg tccaacgatg acaagcggct
     2581 ggacagcgtg ctttcccagc tggacgatct gaaagtccag ctgaaagagg cccaggccga
     2641 ggccgaacag gccaagtacg acgccgagca cgccctcgaa agcgccgaga agaagcgcga
     2701 cgccttaatt aaaaaaggtg aggaagaact cgaaaacgcc cgccgccagg cccatgacct
     2761 gatgcagcag gtgcagaacg aggcgtacag cctgaccgat gaattgcgcc gcatccagaa
     2821 ggacgaaaag accagcgccg cccagcgtgc cgtgcgtgcc cgggaaattg cccgcaagga
     2881 caccgagaca ttgctcaaaa agaccgacgc caagccgaag ccggtcaaag agttcgtccc
     2941 actcaaagaa gtgcagatcg gccaggaggt cgtcattgcg gatcttggcc agacggccac
     3001 cgtcacggcc cgccccgacc gcaacaatat ggtcgaggtc cgcgccggca tcatgaaaac
     3061 gaaggtgccg ctctccaacc tgcgtgcacc ggacaagatg gagaagcgca agcccgccga
     3121 accccaccgc tccacccgcg tgcagctgga taaatcccgc aaggccagta tggaaatcaa
     3181 cctgcttggc tacacggtgg acgaggcctt gaacgaggtg gacaagttcc tggacagcgg
     3241 tatgctgcgc gggcagacga cgctctacat catccacggc aacggcaccg gcgcactgcg
     3301 cacggccatc caaaagcacc tgcgcaccca caaggcggtc aagagtttcc gccccggccg
     3361 ctatggcgag ggcgaaaacg gcgtaactgt ggtggaatta aagtaaaaat tcaaaacaat
     3421 cctgcatttt tgtctatggc aatcattcgc cacatctgct atgctgaagg tagcatgatg
     3481 ggtgtctgaa cattaaatca acaagaaagc gagcgaacgc tttggcaatg gattataaca
     3541 gcacgcgcag ctttacgatg acgctggcgc accgcgcggt gggggatatc cgccgcgggg
     3601 gcttccggca gctgcgcaac tatgtggata tgtgcgccac actggcaaaa aagccgcagc
     3661 agaaagactt ctttgcctac gcgcaaaagg ctttgcagcg cacggacagc tgctattaca
     3721 gcatgatcca ccgtctgctg gactcggtgg aggaggaccg catctgcacc gtcggcgtta
     3781 atatgggctt cggcgggctg atctacggtg ccagcgaaat gaaaaagcag gcggacgtgg
     3841 acggcaaacc catcgcctgg atcacggcgg cccgctgcgg cgacgaacgc ctggcagata
     3901 tggtacccaa ggcggtaaaa aaaggcagct tcgtctggct gctggacgcc acagccaacg
     3961 accctgtgca ggccgtatcg cttgctaagg ccaacccgca gagtgctttc ggcctgctgg
     4021 ccgaccccgc cgcgctggat gatgcctgcg tcaacgcatt gacggcctgc cgcaacctcg
     4081 tcgtcatgcc gctattgcag acgccggaac tgacggcgga ggcctgccgc gccgcccgtc
     4141 gcctaaaagc gcaaaaaatg ttctatgctc tgacggtgct tgtggacgat gacaccgttg
     4201 atgaggtcac acaggacgat tggctcgaga gcattgctca ggaaacactt ttctgtatgt
     4261 gcgcccgcaa gcccggtatt tcggcggaaa gctcccacaa gctgcgccgc agcatcgtgg
     4321 atggccgcat ggagaccggc aagccggttc tgctgctgga ctgggacggc gatgtcaaat
     4381 acctgaacaa ccgcatttcc gagcatatga ccttcggctc cgtgctggca gaaggggaga
     4441 ccttcccgct gcagttgggc tgaaaagttc gcatacttca acaaaaagcg accctgtacc
     4501 gatggtaaaa tcgggtacag ggtcgctttg tttattgtgt ctcgccgcgc agcaggcttt
     4561 gcatgatctc gtggttgaac agcggaatgg ccaaatcgga taccgccgcc agggtgttgc
     4621 tcttcatccc gccgaacacc cagggaatca gcacgcccat catcgcgccg gaaaagaact
     4681 ggccaatttt tgctgcctcc tcggcaggga tatccacttt cagctcccgc gcattctgcg
     4741 caaagatgga gcagagataa tcgaacatat attgctgcaa gctgttctgt ccgttgtagg
     4801 ccaaggcgtt gcagtagaac actttgtttt ccaggaaata ggcttccagt gctgttagca
     4861 gggaatccca gctggaatag gcgctgcgct ctaccaaaaa ctgggtgatg ccactctgga
     4921 aataccagtt caccaagtcg tatttatcct caaaatggta gtagaaggtg ttgcggccca
     4981 cccctgcgtg gtcaacgata tcattgaccg agatcttttc cagcggggtc gtagccatca
     5041 attcctgcag gctctgggcc agcagatgtt ttgtctgatt ggaacgcggc atagcaattc
     5101 tcctgcttta tcggtggtaa aattacgtat aatcattgta gcacatttcc gacaaaaagt
     5161 ccaaatattt cactgccacg ccgcaaaaac tgcggcgggt tttgtatgac aattcagcca
     5221 gaatgtttgg ttttgcccat agcggcgcgg tttgcgggct ttaaattgaa cactccccgg
     5281 cttttgtcta tgtacacaac gccgcgaaaa agcataaaat aaggccataa aacggagaaa
     5341 gaaaaggaga gcaatctatc atgccgatga ataaagtggt tcatgccgcc cagcgtgcag
     5401 cgttcagcgc tgccattgat gccgccatca acgcagtgcg cggcaaaggc cctgaaaata
     5461 tggccgcgca ggctgtcaag ctcgtcaacc tggcgcagcc cctgctggct caccgttacc
     5521 ccgatgccga ctgggacaag gtccgctcct tcgtgtccga ccccaacagc aagtggatgg
     5581 agtacgccta caaggccatc aatgagatcg acccccatat cctgaagatg aacaccctga
     5641 acctcgtgta cgagggtatg ttcgcaggct acaactacgt catggagctg cgcaagaagt
     5701 acgactgcaa tatgccgtgg attttgctgt tcgacccgac ttccgcctgc aacctgcact
     5761 gcaagggctg ctgggctgcc gagtacggca accgcctgaa cctgagtttt gaggatatgg
     5821 atcgcatcgt taccgagggt gaggaactgg gcatccactg gtatatgtgc accggcggtg
     5881 agccgacctg ccgcaaggag gacctgttca agctggccgc caagcaccag aactcggtgt
     5941 tccacctctt caccaacggc acgctgattg accaagcctt ctgcgaccgc gtcaaggaag
     6001 tcggcaacat ggccttcttc atctccatcg agggcatcgg cgatgccacc gacgaccgcc
     6061 gcggcgaggg cgtctatgac cgcgtcatgc acgccatgga tctgatgaag gagaacggcc
     6121 tgctgttcgg tacctccatc tgctacacca gcgctaacta caaggccgtc accagcgatg
     6181 agttcatgga tatgctcatc agccatggcg tgcgcttcaa ctggtacttc cactatatgc
     6241 ccatcggcga tggtgccaat gttgacctga tgctgaacgc tgagcagcgc gagtatatga
     6301 tccaccgtgt ccgtgagatc cgcggctaca ccggcggcaa gcctatcttc tgcatcgact
     6361 tccagaacga cggcgagtac atcgacggct gcattgccgg cggtcgtcag tatgcccata
     6421 tcaacccggc cggtgatgtg gagccctgcg tgttcatcca ctactccaac gccaacatcc
     6481 acgacaagag cctgctcgag tgcctgcagc agccgttgtt caaggaatac cacaagggcc
     6541 agcccttcaa ccacaaccac ctgcgcccct gcccgatgct cgaaaacccc gagctgctgg
     6601 gtgagatggt caagcgcagc ggtgcccaca gcaccgatat gcagcagccg gaatctacgg
     6661 atgatgtgtt ccgccgctgc cgcccctatg ccgaccagtg gactccctcc gctgaccgca
     6721 tctgggccga ggagcacccc gactgtgcct cctgcgccag ctgctccgcc tgcgcatcca
     6781 agtaaaatag cataagttcc tgcgccccgc tgtattttgc agcggggcgt ttttgcgttg
     6841 aaaggcatcc tcctcggggg ggaggcaccg ggcttgtcat gttttcggac atttcaccgt
     6901 tcccggattt tctcacccgg ccgcgcatat agtggtaaca gagccaatgc aggggaggtg
     6961 ggcctatcaa aacggtagtg tatctggatg tgctgctgct gacgaatttc ctcatggcct
     7021 atttcctgtt gctggcggca ggggcgctca gcgggcagcg gggagggttc gtgcggatgc
     7081 tggcgggcag tgggctggcg gcgatgtcgt cgcttatttt gtttgcgccg ccccagccct
     7141 acctggtgca ggtcgtctac aaaatcacca cggcgctggc catcacggcg gcggctttcg
     7201 gctggcgcag caaacgacgg ctggtcacgg cggcctgttg gtatgcagcg ctgaatatcg
     7261 ccctggcggg ggcggtgctg ctggccgttc tgcgcaccgg cacgcgggca gcggatacgg
     7321 ccaacctgac ggtgtatctg cgcgtttcgc cgctgctttt attggcgctg tcgggcgtct
     7381 gctgcggcg
//
LOCUS       DBMDSQ010000068         7384 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_2671_length_7384_cov_1.847041, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000068 DBMDSQ010000000
VERSION     DBMDSQ010000068.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 7384)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7384)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7384
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2671_length_7384_cov_1.847041"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..412)
                     /locus_tag="ACZPAX_03815"
     CDS             complement(<1..412)
                     /locus_tag="ACZPAX_03815"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007048324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HIR1113055.1"
                     /translation="MKQTGIFNGRAEVLYNYGRFGWTRGGGKVWHGGIDLVGLDDTTV
                     RMPYYKGKRITGRVVRARIVTDRRDKTWEWGWYVCVQLDTAQTPDAVNFLYFAHCEKL
                     LVQAGQTVTSGDALAVMGNTGNAAGGYKHCHLEVR"
     gene            complement(445..885)
                     /locus_tag="ACZPAX_03820"
     CDS             complement(445..885)
                     /locus_tag="ACZPAX_03820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113056.1"
                     /translation="MSATRNPFYQLPGGADLPGDVRVSDTADVTKTAAAGWAASPAAV
                     AAAESIARPDYTNYTKLGKKGTFTIDTPGFVQFCCSTGSTEASIFHRIKINGVIVDQG
                     HTESRNYTYYWSPLFPVSPGDTIETIGEPENLGYNTYFYNLRKS"
     gene            complement(905..1318)
                     /locus_tag="ACZPAX_03825"
     CDS             complement(905..1318)
                     /locus_tag="ACZPAX_03825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113057.1"
                     /translation="MNAAQNPLNACPYWVGDILVTMSEIQPAQRWPGTSWEQITDCML
                     RAADDTHPAGSTGGAWEVVQTLEQMPRHNHAFATYQDGYSTGTHGADTYITPVGRKPY
                     SADWYGGTESAYNGGSQPMPIENKYTAVYMYRRTG"
     gene            complement(1425..1784)
                     /locus_tag="ACZPAX_03830"
     CDS             complement(1425..1784)
                     /locus_tag="ACZPAX_03830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113058.1"
                     /translation="MNATTNPYAVTPPDKTLSVEGVAADAKAVGDELSTRTVKYLGSV
                     VDTGETNSATYDMSGKPGGLYMVCFFRSNLIFILFLKDDSTLEWTADSNAMTITGSKV
                     TFKNLNWYDSGYLFRLA"
     gene            complement(1796..2245)
                     /locus_tag="ACZPAX_03835"
     CDS             complement(1796..2245)
                     /locus_tag="ACZPAX_03835"
                     /inference="COORDINATES: protein motif:HMM:NF046467.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate protein"
                     /protein_id="HIR1113059.1"
                     /translation="MTDNPYRYPYVPVGGIIEWDGTGLTGAPDLSTPEKVAAVYGYGT
                     WERYGTDRVTVGAGGEYEAGATGGEKAHTLTVAELPKNIGHLNALSWASNNSQTDGCF
                     SVSQKHQDKTMPSGETVGDALYTLSGKGQSHNNMQPYISTYRYRRIK"
     gene            complement(2242..3423)
                     /locus_tag="ACZPAX_03840"
     CDS             complement(2242..3423)
                     /locus_tag="ACZPAX_03840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113060.1"
                     /translation="MTEIALTNGVKVPADSLAFWRFAWMDGNGATDRPIRLVQYDDTL
                     PVAAVTLKYYDQEGQEQTYIPPTGATLRVKMRKPDEKWVLNDCLGVDAEGRVYFAFTL
                     QMCAADGTGAWCNIQVTTENGTKHCQKINVQVERNAVQLDDITSTDEYRTLESLAKDV
                     TDAAKIMKDNEAAIQSAYANMDAIKAAPENATAAAASAKEARSWAVGDTGTRQGEGMD
                     NAKYYAALAQQVSQGAVGWYPNYEALYAAHEVGYDGNWAIIGDTDTIWVWDSDTGTWR
                     DTGESSKFANYYDKTQIDTMLAAMKPNLKTVTVAASAWTTGSYSVTWDDGTSTTYTAC
                     ATVEVADITTDSKISVSPRTCVTDSVRLVAALEPASGSVKFYANAVPTGAATFILEVS
                     V"
     gene            complement(3420..3632)
                     /locus_tag="ACZPAX_03845"
     CDS             complement(3420..3632)
                     /locus_tag="ACZPAX_03845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113061.1"
                     /translation="MSDNMNIITMQDEVVEALNRHSLPLAVKKIVLENVLLRVEAAMR
                     DVMERESTETTAAEGAAPETEAGGEA"
     gene            complement(3643..5415)
                     /locus_tag="ACZPAX_03850"
     CDS             complement(3643..5415)
                     /locus_tag="ACZPAX_03850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113062.1"
                     /translation="MQTTSELWKKTFANLNHTGELRVVIGGVEYGAGSLKEAKATRFL
                     FDEAPGVGNACAAMLDLTIVPKGTIPRAAKVVAEYRLVLNRRPTEWMPKGTYWINTRG
                     TSGRYVTLECCDAMLKAEQTFLTESTTDNWPKAATAVAAEIATRMGTPLDAATTALVK
                     ARNYNVGLPVNDYTMRDVLCHMAAQMGGNWCVTEDGHLRLVPLAGGTVNAGAVGGRPD
                     MGTVQTVTGLLLWQDDNSAIEKGTDTGLLLEADQPDATDALTTTALGYVKGLNYQPYS
                     STQRHIDPAAELGDLADIGTHKGLLICSLTETLGKSYYADVSAPERAAEAEQEFPYLT
                     KTERTAKRIASCPTRSEMQSEISKNNESIELRITKIDTRCGTLETDYNQLNQNYTSLK
                     TSYNEISAVMVRDGEVRSKFALDTSSCTISAGTVTFKGNTLIVDSTNFKVNAAGDVTI
                     TGTFESKDDTSRVWTINGCMNFYRKVSGSWVQGGSLQALGSNAACGRLDLYGPSAGGA
                     TTKQSNIEMMGDWGGGKINCYAANGKKIFEFFPGDYGNGQFRLFKGDGTILAEAYQQS
                     DGKARMKVAGTIECEKVLRNGTYL"
     gene            complement(5419..5787)
                     /locus_tag="ACZPAX_03855"
     CDS             complement(5419..5787)
                     /locus_tag="ACZPAX_03855"
                     /inference="COORDINATES: protein motif:HMM:NF042749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6711 family protein"
                     /protein_id="HIR1113063.1"
                     /translation="MTFTINGTDIAPYIKAGSLKVTQHKISTDDSGRDTQDGTMYNTV
                     IAKKYDIEAECRALKTDEASVVLTALMENDWVTVAFTSPVTGGEESVTMYCTEPSATH
                     AMRKNGVDYWTGIDIKLVER"
     gene            complement(5797..>6370)
                     /locus_tag="ACZPAX_03860"
     CDS             complement(5797..>6370)
                     /locus_tag="ACZPAX_03860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113064.1"
                     /translation="KESVSEAWNNIIGGIKQQWENFKNDPLKWGEDMLRTFAQGITNG
                     AKWVIEKVKGIGSDIRSLLHFSVPDKGPLADADTWMPDMMQLMADGVQQKKKLLLQKM
                     QGLAGEMSGQMNANLTGAVAASVQTAGNAGMPDNSAAIITVLENGFRVLRRAIEDKET
                     SVQLDGETVSRSVTQRQNRRELMRGKAVTP"
     assembly_gap    6371..6380
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6381..7303)
                     /locus_tag="ACZPAX_03865"
     CDS             complement(<6381..7303)
                     /locus_tag="ACZPAX_03865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113065.1"
                     /translation="MMAALFGTTVQAAASTASKVNAAATGASSKLKRSVMSFDKLNKV
                     DSKDSGGGSGSSTGIDPTFSGEDVGVPPWMKKIQEGFKNLGEGCKALKENFLEAWNMD
                     GVGDRITAAFGNIGNRLLDCWVNITGSIREWCENLDFSPLLNSFADLMEALEPVTDLL
                     CGALEWAFDNVLLPFGKWTIEAALPAILEALAGALDLLVAVCNALKGPAQWVWDNFLQ
                     PLAAWTGSALILAVRAVGAAFEWLAGLISGDKEKMEKAAAHMSEAWENFKNKTVEVFG
                     NMWSIIHSGYEAFEQWMLDKTTQLADDWIAK"
BASE COUNT         1287 a         2327 c         1896 g         1864 t
ORIGIN      
        1 cccgcacctc cagatggcag tgcttgtagc cgcctgccgc attgccggta ttgcccatca
       61 cagccagcgc gtcgccgctg gtgaccgttt ggcccgcctg caccagcagt ttttcacagt
      121 gggcaaagta caaaaagttc acggcgtcgg gcgtctgggc ggtgtccagc tgcacgcaga
      181 cgtaccagcc ccactcccag gttttgtcgc ggcggtcggt cacaatgcgc gcccgcacca
      241 cgcggccggt gatgcgctta cccttataat agggcatccg cacggtggtg tcgtccagcc
      301 ctaccaggtc aatgccgccg tgccagactt tgccgccgcc ccgcgtccag ccaaatcggc
      361 cgtagttgta cagcacttcc gcgcggccat taaagattcc ggtttgtttc actttttcgt
      421 cctttctccc cgcatgggcg gggattatga ttttcgcaag ttgtaaaaat atgtattgta
      481 gcccaaattt tctggttcgc caatggtttc gatagtgtca ccaggtgaaa cgggaaacaa
      541 aggagaccag tagtatgtat agtttctgga ttctgtatgg ccttggtcca cgatgacgcc
      601 attgatctta atacggtgga aaattgatgc ctcagtagac ccggtcgagc aacagaattg
      661 tacaaatcct ggtgtgtcaa tggtaaaagt gccttttttg ccgagtttag tgtagtttgt
      721 ataatcaggt ctggctattg attcggctgc tgccaccgcc gccgggctgg ccgcccagcc
      781 ggccgcagca gttttcgtaa cgtccgccgt atcactcaca cgcacatcgc cgggcaggtc
      841 agccccgccg ggcagctggt aaaaggggtt gcgggttgcg ctcataccgc cacccccttt
      901 ggggtcagcc ggttcgacgg tacatataca cagccgtgta cttgttctcg attggcatgg
      961 gctggctgcc gccgttgtat gcgctttctg ttccaccata ccaatccgcg ctgtaaggct
     1021 ttctgccgac cggggttatg tatgtatctg ccccatgggt gcctgtagaa tagccatctt
     1081 ggtaagtcgc aaaagcgtga ttgtgccgtg gcatctgttc cagtgtctgc accacttccc
     1141 acgcgccgcc ggtgctgccc gccgggtgcg tatcgtccgc agctctcagc atacagtcgg
     1201 tgatttgctc ccagcttgtc cccggccagc gctgtgccgg ttggatttcg ctcatcgtca
     1261 caagtatgtc gcccacccag tacgggcagg cgtttaaggg gttctgtgct gcgttcatac
     1321 ccagcacctg tgcaaactgc acaaggctgt tttatggcag ccccccccga acggataacg
     1381 tagtttcgtc attttgatgc acctccaaaa ttttttaatc tttactaggc caatctaaat
     1441 agatacccgc tgtcgtacca gttcaaattt ttgaaagtaa ctttactgcc agtgatagtc
     1501 atagcgtttg agtccgcagt ccattctagc gtactatcgt ccttcaaaaa caatatgaaa
     1561 attaggttcg acctaaaaaa gcagaccatg tacaagccgc ctggtttccc gctcatgtcg
     1621 taggtggcgc tatttgtttc gccggtatct actacggaac caagatactt gactgttcta
     1681 gtggacaatt catcacccac agccttagca tcagcggcaa cgccctcgac gctcagcgtt
     1741 ttgtcggggg gggtgacagc atacggatta gttgttgcgt tcatgatgtt ctcctttact
     1801 taatgcggcg gtagcggtag gtgctgatgt acggttgcat gttgttgtga ctttgaccct
     1861 taccagataa ggtatacagt gcatcaccaa ccgtctcacc agatggcatt gttttatctt
     1921 gatgtttttg tgaaactgaa aaacagccgt cggtctgtga gttattagat gcccatgata
     1981 aagcattaag atggccaata ttttttggta attcagcaac agtcagcgta tgcgcctttt
     2041 cgccgccagt agcaccagcc tcgtactccc cgccagcacc caccgtcact cggtcagtac
     2101 cgtatcgctc ccacgtgccg tacccataga ctgcagccac cttttccggc gtactcaaat
     2161 ccggtgcgcc agtcagcccc gtgccgtccc actcgatgat gccgcctacc ggcacataag
     2221 ggtatctata cggattgtcc gtcatacgct cacctccaga ataaacgttg ccgcgccggt
     2281 cggcacggca ttggcgtaga atttcactga accggatgcc ggctcaagcg ccgctaccaa
     2341 acgcacacta tcggtcacac aggtgcgtgg gcttacgctg attttgctgt cggtggttat
     2401 gtcagcaact tctacggtag cgcaggcggt gtaggtggta gacgtgccgt cgtcccacgt
     2461 cacgctataa ctgccggttg tccaggccga cgccgccact gtgaccgttt ttaagttcgg
     2521 cttcattgcg gccaacatcg tgtcgatttg cgtcttatca taataattcg caaacttgct
     2581 gctctcgcct gtatcgcgcc aagtgccggt atcagaatcc caaacccaga tggtatcagt
     2641 gtcgccaata atggcccagt ttccatcgta gccgacctcg tgcgccgcgt acagcgcctc
     2701 gtaattcggg taccacccca ccgccccctg actgacttgt tgggcaagcg cagcgtagta
     2761 tttggcgttg tccatgccct cgccctggcg cgtgcccgta tcgcccacgg cccaactgcg
     2821 ggcctccttg gcgctggccg cagcggcggt cgcattctcc ggggccgctt tgatggcgtc
     2881 catgttcgca tacgcgctct ggatagccgc ctcgttgtcc ttcatgattt ttgcggcgtc
     2941 ggtcacgtcc tttgccaggc tttccagcgt gcggtattcg tcggtcgacg taatatcgtc
     3001 caactgtacg gcgttgcgct ccacctgcac attgattttc tggcagtgct tcgtgccgtt
     3061 ctcggtcgtc acctggatat tgcaccatgc gccggttccg tccgccgcgc acatctgcaa
     3121 ggtaaaggca aagtacacgc gcccttcggc gtccacgccc aggcagtcat tcagcaccca
     3181 cttttcatcc ggcttgcgca ttttcacgcg cagcgtcgcg ccggtagggg ggatgtaggt
     3241 ctgctcctgg ccttcctggt cgtagtattt cagtgttacg gcggccaccg gcagggtgtc
     3301 atcgtactgc accagccgta tcggtctgtc ggtcgcaccg ttgccgtcca tccaggcaaa
     3361 ccgccagaat gccagcgaat ccgccgggac tttcacgccg tttgtcaggg ctatttccgt
     3421 cacgcttcac cacctgcttc cgtctccggc gctgcgccct ctgccgccgt ggtttcggtg
     3481 ctctcgcgct ccatcacgtc acgcatggcg gcttccaccc gcagcagcac attttccagc
     3541 acgatctttt tcaccgccag cggcaggctg tgccggttca gcgcttctac cacttcatcc
     3601 tgcatcgtga taatgttcat attgtcgctc atagctttca cctcacaaat aggttccgtt
     3661 ccgcaagact ttttcacatt caatcgtacc ggccaccttc atgcgcgcct ttccgtcgct
     3721 ttgctggtac gcttcggcta aaatcgttcc gtcgcccttg aacagccgaa actgtccgtt
     3781 tccgtaatcg ccgggaaaaa actcaaaaat cttttttccg tttgccgcgt aacagttgat
     3841 tttaccgccg ccccaatcgc ccatcatttc aatgttggat tgtttggtcg tcgctccgcc
     3901 ggcactcggc ccatacaaat caagccgccc gcaggccgca ttgctcccca gggcttgcag
     3961 gctgccgccc tgcacccagc ttccgcttac cttgcggtaa aagttcatgc agccgtttat
     4021 ggtccacacg cggctggtgt cgtctttcga ctcaaacgtc ccggtaatcg tcacgtcgcc
     4081 cgcggcgttc actttaaaat tcgtgctgtc cacgatcagc gtgtttccct tgaacgtcac
     4141 cgtcccggca gaaatcgtgc agctgcttgt atccagcgcg aacttgctgc gcacctctcc
     4201 gtcacgcacc atcaccgccg aaatttcgtt gtacgacgtt ttcagcgatg tatagttctg
     4261 gttcagctgg ttgtagtcag tttccagtgt gccgcagcgc gtgtcgattt tcgtgatgcg
     4321 cagttcaata gattcattat tcttgctgat ttcgctctgc atctcgcttc gcgtcgggca
     4381 cgatgcgatt cgctttgcgg tgcgctcggt ctttgtcaga taggggaact cctgttccgc
     4441 ctccgcggcc cgctcggggg cgctcacgtc ggcatagtag ctcttgccca gggtctccgt
     4501 cagggagcaa atcagcagtc ccttgtgcgt cccaatgtct gccaggtcgc ccagctcggc
     4561 cgccgggtca atgtgccgct gggtgctgct gtacggctgg tagttcagcc ccttcacata
     4621 gcccagcgcc gtggtcgtca gcgcatccgt tgcgtccggc tggtcagctt ccagcagcag
     4681 gccggtgtcc gtgccttttt caatggcgct gttgtcgtct tgccacagca gcaggccggt
     4741 cacggtctgc accgtgccca tgtctggccg cccgcccacg gctccggcgt tcaccgtgcc
     4801 gcctgccagc ggtacaagcc gcaggtggcc gtcctcggtc acgcaccagt tgccgcccat
     4861 ctgggccgcc atgtggcaca gcacatctcg catcgtgtaa tcgttcaccg gcaggcccac
     4921 attgtagttg cgggccttta ccagcgccgt ggtggccgcg tccagcggcg tgcccatccg
     4981 ggttgcgatt tccgccgcca ccgccgtggc cgccttcggc cagttgtccg ttgtgctctc
     5041 ggttaaaaat gtttgctcgg ctttcagcat ggcatcgcag cattccagcg ttacataccg
     5101 tccgctcgtg ccccgggtat tgatccagta ggttcccttg ggcatccact cggttggccg
     5161 tctattcagc acaagccggt actcggccac caccttggcg gcgcgcggta tcgtgccctt
     5221 ggggactatg gtcaaatcca gcatggcggc gcaggcattg cccacgcccg gcgcttcatc
     5281 aaaaaggaag cgcgtagctt tcgcctcttt caggctgccc gcgccgtatt ccacgccgcc
     5341 gatcactacg cgcagttcgc cggtatggtt caggttggca aacgtttttt tccacagttc
     5401 gcttgtcgtc tgcattttct acctctccac cagcttaatg tcaattccgg tccagtagtc
     5461 cacgccgttc ttgcgcatgg cgtgggtcgc gctcggctcg gtgcagtaca tggtcacgct
     5521 ttcctcgccg cctgtcacag ggcttgtaaa ggccaccgtc acccagtcat tctccattaa
     5581 ggcggtaagc actacgcttg cctcgtcggt tttcagtgcc cggcattctg cctcaatatc
     5641 gtatttcttg gcaatcacgg tattgtacat tgtgccgtcc tgggtatcgc gtccgctgtc
     5701 gtcggtgctg attttatgct gcgtcacctt cagcgacccc gccttgatgt agggcgctat
     5761 gtccgtgccg ttgatggtaa atgtcatagc ctctccttac ggcgttacgg ccttgccgcg
     5821 catcagctcg cgccggttct ggcgctgcgt cacgctgcgg cttacggtct cgccgtccag
     5881 ctgcacgctg gtttccttgt cctcaatggc gcggcgcagc accctaaagc cgttttccag
     5941 caccgtgata atggcggcgc tgttgtccgg cataccggca ttgcccgcag tctgcacact
     6001 ggcggccacg gccccggtca ggttcgcgtt catctggccg ctcatttcac cggccaggcc
     6061 ctgcatcttt tgtaacagca gtttcttttt ctgctgcacg ccgtcggcca taagctgcat
     6121 catgtcgggc atccaggtgt cggcgtcagc cagcgggcct ttgtccggca cactgaaatg
     6181 caaaagcgag cgaatgtcgc tgccgatgcc ttttactttt tcaattaccc actttgcgcc
     6241 gttggtgatc ccctgtgcga acgttctcag catatcttcg ccccatttca gcgggtcgtt
     6301 cttgaaattt tcccactgct gcttgattcc gccgataatg ttgttccagg cttcgcttac
     6361 gctttctttc nnnnnnnnnn cacttggcga tccagtcatc tgcaagctgt gtggttttgt
     6421 caagcatcca ctgttcaaac gcttcgtacc cgctgtggat gatgctccac atattgccga
     6481 aaacttcaac cgtcttgttt ttgaaatttt cccaggcctc cgacatatgg gcggcggcct
     6541 tttccatttt ttctttgtcg ccgctgataa gtccggccag ccactcaaac gccgcgccca
     6601 cggcacgcac cgccaaaatc agcgcactgc ctgtccacgc cgccaacggc tgtaaaaaat
     6661 tgtcccatac ccactgtgca ggccctttca gcgcattgca gacagcgacc agcaggtcca
     6721 gcgccccggc cagtgcttcc agaatggcgg gcagggcggc ttcaatggtc cacttgccaa
     6781 acggcaacag cacattgtca aaagcccatt ccagcgcacc gcacagcagg tccgtcaccg
     6841 gttccagcgc ttccatcaaa tccgcaaagc tattcagcag gggagaaaag tccaaattct
     6901 cacaccattc ccggatagac ccggtaatgt tcacccagca gtccagcagg cggtttccga
     6961 tgtttccgaa cgccgcggta attctgtcgc ccacgccgtc catgttccac gcttccagaa
     7021 aattttcttt cagcgccttg cagccctcgc ccaggttttt gaacccctcc tggatttttt
     7081 tcatccaggg cggtacgcct acgtcctcac cgctgaacgt cgggtcaatg ccggtgctgc
     7141 tgccgcttcc gccgccgctg tccttgctgt ccaccttgtt caacttgtca aagctcataa
     7201 cgctgcgctt cagcttgctg ctggccccgg tcgctgcggc gtttaccttg ctcgcggtgg
     7261 acgcggcggc ctgtaccgtc gtgccaaaca gtgccgccat catactggca aactgcgctg
     7321 tcaccgttgc aatggcgctg ctcaacgtcc gcagcatcgg caaaatggcg tttatcaccg
     7381 gcgc
//
LOCUS       DBMDSQ010000069         7383 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_2672_length_7383_cov_1.876545, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000069 DBMDSQ010000000
VERSION     DBMDSQ010000069.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 7383)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7383)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7383
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2672_length_7383_cov_1.876545"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..883
                     /locus_tag="ACZPAX_03870"
     CDS             <1..883
                     /locus_tag="ACZPAX_03870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140327 - flippase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HIR1113066.1"
                     /translation="FGVVAIAFWYKYRSAVFAPLPASQLGGIARQTVPYGVALAPAQI
                     LFSLSSGICLSFVGNACGENAQGLFAFGYSLAQLVTAIQAGFSTYWGPYVYAHYREEQ
                     ERICRVHDVLNLLIFSFFCVLVMFEDIVFVIFPDKRGCLAIFPLLMLAVVFNILCEGT
                     VYGNSIARKPWHDTIGIGLGALSNLGLCAVLVPAFGLTGAALALAASNGIAFLYRSVT
                     GQMYYRTVASYAKTISGFLLAFAVTAIGTLLWQHFIVKFALVGVILFIYCTLYRAQLA
                     KLWGIGLGILRSIFHRK"
     gene            893..1471
                     /locus_tag="ACZPAX_03875"
     CDS             893..1471
                     /locus_tag="ACZPAX_03875"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007048236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR1113067.1"
                     /translation="MKHCGTQELTTERLVLRRLSIDDSEMMYNNWARDKQVTKYLRWN
                     AHRSWGETAETLNEWEKHYNDPKFYQWGITDKHSEVLMGTISLCPAPMLKTGWHLNTE
                     RLGAPWEVGYALGRKWWNKGYMTEALCAVRDYWFDTVGADWLAAVHANENIASAAVLE
                     KAGFVYDHDVTDRKFDGTPVPCRAYHLLKEEL"
     gene            1468..2757
                     /gene="hflX"
                     /locus_tag="ACZPAX_03880"
     CDS             1468..2757
                     /gene="hflX"
                     /locus_tag="ACZPAX_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007048237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HIR1113068.1"
                     /translation="MSDNTTELDFGTTAQKTTDAEKLPVVLLALDQGKYDMTRSLDEL
                     RALADANGMDAVAEVVQKRSTPEAATLLGEGKVAEARLVCQNVNAAAAIFDGELTGSQ
                     IRNLSAALQVEVLDRTMLILEIFRARATTNEGKLQTELATLRYQMPRLQGLGESLSRQ
                     GGGGGGGGGARRGAGETKLELDRRHLHHRIEHLEGRLKELEKRRGETRRARQKNNVPV
                     VALVGYTNVGKSSLLNALCGEQIFEADMLFATLDPTARKLVLPSGLQIILVDTVGFVS
                     RLPHHLVEAFKSTLEEAAFADVIIKVADAGDAQAAEQLAVTDEVLGSLDCDGIPQLVV
                     YNKCDVANAVAFDPDILLTSAKTGYGLDALLAKIDEVLNHRVRTIEVVLPYDKLALAD
                     ILRSRGSVAAEEYREDGVFYRATVKIDDLHRFEEYLL"
     gene            2966..4408
                     /gene="gltX"
                     /locus_tag="ACZPAX_03885"
     CDS             2966..4408
                     /gene="gltX"
                     /locus_tag="ACZPAX_03885"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007061951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HIR1113069.1"
                     /translation="MSTVRTRFAPSPTGYMHVGNLRTALYTYLQARHNGGTFILRIED
                     TDQGRLVEGATDIIYNTLKATGLTWDEGPDIGGPVGPYVQSERMGMFKQYAEQLVKAG
                     KAYYCFCTEERLNEMHEAQRAAGEMTHYDGHCRSLSAEEVAAKLAAGEPYVIRQKIPE
                     SGMAGFDDVVYGHIEVDVKELDDQILIKTDGMPTYNFANVVDDHLMGITHVIRGSEYL
                     SSTPKYNLLYDAFGWEKPVYIHCPPVMKDAQNKLSKRNGDASYQDLVAKGYLPAAVLN
                     YLLLLGWAPEGEQEIFSLDEMIKIWDPSRISKSPAIFDPLKLRAINAAYIRALPAEEF
                     RRLADPFIDSAVHCDIDRDLLCANLQPRCEVLEDIPPQLDFFDAVLPIDAEMYRNKKQ
                     KTTPESAKEALTALLPVLEAQTDWTRDAIFEACKTLAEQMEKKNGWLLFPLGIALSGK
                     ARTPGGGTDLAAMMGKAETLKRVKDALGKL"
     gene            complement(4489..4920)
                     /locus_tag="ACZPAX_03890"
     CDS             complement(4489..4920)
                     /locus_tag="ACZPAX_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015565253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RrF2 family transcriptional regulator"
                     /protein_id="HIR1113070.1"
                     /translation="MMISTKGRYALRVMIDIAENQHDGYIPLKEIAARQEISEKYLEA
                     IVKSLVRARYLTGLRGKSGGYRLTKTPGEYTVGNILRLTEGSLAPVACLGQNPVECPR
                     MVECRTLPMWRKLNTMINDYFDGITLADLVNTAQPGNDYVI"
     gene            5186..6118
                     /gene="cysK"
                     /locus_tag="ACZPAX_03895"
     CDS             5186..6118
                     /gene="cysK"
                     /locus_tag="ACZPAX_03895"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005936447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HIR1113071.1"
                     /translation="MSKIYTSADQLIGRTPLLELTHIEAAEGLDAKILAKLEYFNPAG
                     SVKDRIAKAMIDDAEATGKLKPGSVIIEPTSGNTGIGLASVAAARGYRIIIVMPETMS
                     VERRQLMKAYGAELVLTDGSKGMKGAIAKADELAKEIPNSFVPGQFVNPANPDAHKRT
                     TGPEIWEDTDGKVDIFVAGVGTGGTVTGVGEYLKSQNPAVKVVAVEPATSPVLSKGTA
                     GAHKIQGIGAGFVPDVLNTKVYDEVIPVANEDAFATGKLVGHKEGVLVGISSGAALWA
                     AIQLAKRPENKGKTIVALLPDTGDRYLSTPLFAD"
     gene            6330..>7383
                     /gene="mnmA"
                     /locus_tag="ACZPAX_03900"
     CDS             6330..>7383
                     /gene="mnmA"
                     /locus_tag="ACZPAX_03900"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005929358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HIR1113072.1"
                     /translation="MTTTPGALIAMSGGVDSTVAAYLMKKAGYRCMGATMRLYQNEDL
                     GQSGFHTCCSAKDVEDAADVAFQLDIPFQVVNYTEEFREKVIGKFIATYEAGGTPNPC
                     IDCNRTMKFDKMLDFARENGLDYVVTGHYARIEQDPDTGRWLLKKALYADKDQSYVLY
                     VLTQDQLAHTKFPLGGMDKPSIRKIAEEQGFCNARKHDSQDICFVPDGDYVGFMERYT
                     GKYYPDGDFIDRNGKVVGRHHGAVRYTNGQRKGLGLALGAPVYVCGKDMEKNTVTVGP
                     ESALFTTTLIADDFNWISIPELTAPMRVKAKARYRQVEQPATVYPLDNGLVKVVFDEP
                     QRAITRGQSVVLYDGDI"
BASE COUNT         1508 a         2238 c         2194 g         1443 t
ORIGIN      
        1 ctttggcgtg gttgccattg cattctggta caagtaccgc agcgctgtgt ttgcgccgct
       61 gcccgccagc cagctgggcg gcattgcccg ccagactgtg ccctacggcg tggcactggc
      121 cccggcccag atcttgttca gcctgtccag cggtatctgc ctttcctttg tgggcaacgc
      181 ctgcggcgag aatgcgcagg gcctgtttgc cttcgggtac agcctggcac agctggtcac
      241 ggccatccag gcgggcttct ccacctactg gggcccctac gtctacgccc actaccgcga
      301 ggagcaggag cgcatctgcc gcgtacatga cgttttgaac ctgctgatct tcagcttctt
      361 ctgcgtgctg gtcatgttcg aggatatcgt gttcgtgatt ttccccgaca agcgcgggtg
      421 cctcgccatt ttcccgctgc tgatgctggc cgtcgtcttc aacattctgt gcgagggcac
      481 ggtctacggc aactcgattg cccgcaaacc gtggcatgat accatcggca tcgggctggg
      541 ggcgctgtcc aacctggggc tgtgcgcggt gctggtaccc gcctttgggc tgaccggcgc
      601 agcgctggcc ctggccgcca gcaacggcat tgcgtttttg taccgcagcg tcacgggcca
      661 gatgtactac cgcaccgtcg ccagctatgc caagacgatt tccggctttc tgctggcgtt
      721 cgcggtcaca gccatcggca cgctgctgtg gcagcatttt attgtaaaat ttgcgctggt
      781 aggtgtgatt ttgttcatct actgcaccct ctaccgtgca cagctcgcca agctctgggg
      841 catcggcctt ggcatcctgc gcagcatctt ccacagaaag taaggcgaaa ccatgaaaca
      901 ttgcggcaca caggaattga cgacggaacg cttagttttg cggcggcttt ccatcgatga
      961 cagcgaaatg atgtacaaca actgggcccg cgacaagcag gtgacgaagt acctgcgctg
     1021 gaacgcccac cgcagctggg gcgagactgc cgaaacgctg aatgagtggg aaaagcacta
     1081 caacgacccg aagttttacc agtggggcat caccgacaag cattcggagg tgctgatggg
     1141 gaccatcagc ctgtgccctg ccccgatgtt gaaaaccggc tggcacctga acaccgagcg
     1201 cctgggtgcc ccctgggagg tcggctacgc gctgggccgc aagtggtgga acaagggcta
     1261 catgaccgag gccctgtgcg ctgtgcggga ctactggttt gacacggtgg gggctgactg
     1321 gctggccgcc gtacacgcca acgagaacat tgccagcgcc gccgttttgg agaaggcagg
     1381 ctttgtctac gaccatgacg tgaccgaccg caagtttgac ggcacgccgg tcccctgccg
     1441 tgcgtaccat cttttgaaag aggaattatg agcgataaca cgactgaatt agactttggc
     1501 accactgccc agaaaaccac agacgccgag aaactgccgg tagtgctgct ggcgctggac
     1561 cagggcaaat acgatatgac ccgcagcctg gacgagctgc gggcgctggc cgacgccaac
     1621 ggtatggacg ccgtggccga ggtggtgcag aagcgctcga ccccagaggc cgccacgctg
     1681 ctgggcgagg gcaaggtcgc cgaggcgcgg cttgtctgcc agaacgtaaa cgcagccgcc
     1741 gcgatttttg acggtgagct gaccggtagc cagatccgca acctgtcggc ggcactgcaa
     1801 gtcgaagtgc tggaccgcac gatgctgatc ctcgaaattt tccgcgcccg cgccaccacg
     1861 aacgagggca aattgcagac cgagcttgcc acactgcgct accagatgcc gcgcctgcag
     1921 ggcctgggcg agagtttgag ccgccagggc ggcggcggcg ggggcggcgg cggtgcccgc
     1981 cgcggtgccg gtgagacaaa gctggagctg gatcgtcgcc acctgcacca ccgcattgag
     2041 catctggaag gccgcctgaa agagctggaa aagcgccgcg gcgagacccg ccgggcacgg
     2101 cagaaaaaca acgtgccggt ggtggcgctg gtcggctaca caaacgtggg caagtcgagt
     2161 ttgctgaacg ccctgtgcgg cgagcagatt tttgaggccg atatgctgtt tgccacgctg
     2221 gaccccacag cccgcaagct ggtgctgccc agcgggctgc aaatcatcct ggtggacact
     2281 gtcggctttg tcagccggct gccgcaccat ctggtggagg cgttcaaatc aacgctggag
     2341 gaagccgcgt tcgccgatgt catcatcaag gtcgccgacg cgggcgacgc ccaggccgcc
     2401 gaacagctgg ccgtgaccga tgaagttttg ggcagcctcg actgcgatgg catcccccag
     2461 ctggtggtct acaacaagtg cgacgtcgct aacgccgtgg cctttgaccc ggatatcctc
     2521 ttgaccagcg ccaagaccgg ctatggcctg gacgcgctgc tggcgaagat cgacgaggtg
     2581 ctgaaccacc gcgtccgcac cattgaggtc gtgctgccct atgacaagct ggccttggcc
     2641 gatatcttgc gcagccgcgg cagcgtagcg gcggaggaat accgcgagga tggcgttttt
     2701 taccgcgcca ccgtaaaaat tgacgacctg caccggtttg aggaatacct gctttaatcg
     2761 ttctgtaggg gccggacata tccggccttc aactttcccg caaatgcgct cctgcctttc
     2821 ggctgcgggc cgggcatgcc cggcccctac ggtgtgtttt cccgttgaca tctcatagct
     2881 ctgcgtttta taataatttt ggcgggctgt gcccgcacct tatgtattat ctattatctt
     2941 ttatctataa aaaggggtaa taactatgtc taccgtccgc actcgttttg cgccgtcccc
     3001 cacaggctat atgcatgtag gcaatctgcg caccgcactg tatacctatc tgcaggcgcg
     3061 ccacaacggc ggcaccttta tcctgcgcat tgaggacacc gatcagggcc gtctggtcga
     3121 gggcgccacc gacattatct acaacacgct gaaggccacc ggtctgacct gggacgaagg
     3181 ccccgacatc ggcggccctg tcggccccta tgtccagagc gagcgcatgg gcatgttcaa
     3241 gcagtatgcc gagcagctgg tcaaggccgg caaggcgtac tactgcttct gcaccgagga
     3301 gcgcctgaat gagatgcacg aggcccagcg cgcggccggc gagatgaccc actatgacgg
     3361 gcattgccgc agcctgagcg ccgaggaggt cgccgccaag ctggccgccg gggagccgta
     3421 tgtcatccgc cagaaaattc ccgagagcgg catggcgggc tttgacgatg ttgtctatgg
     3481 ccacatcgag gtcgatgtca aggagctgga cgaccagatt ctgatcaaga ccgacggtat
     3541 gcccacctac aactttgcca atgtcgtgga cgaccacctc atgggcatca cccacgtcat
     3601 ccgcggcagc gagtatctgt cctcgacgcc gaagtataac cttttgtatg acgctttcgg
     3661 ctgggaaaaa ccggtttaca tccactgccc gcccgttatg aaggatgcac agaacaagct
     3721 gagcaagcgc aacggcgacg ccagctatca ggacctggtg gcgaagggct acctgcccgc
     3781 cgccgtgctg aattatctgc tgctgctggg ctgggcaccc gagggcgagc aggagatctt
     3841 cagcctggat gaaatgatca agatctggga tccgtcccgc atctcgaagt ctcccgccat
     3901 ctttgacccg ctgaagctgc gcgctatcaa cgccgcctac atccgcgccc tgcctgccga
     3961 ggagttccgc cgtctggccg acccgttcat tgacagcgcc gtccactgcg atattgaccg
     4021 cgacctgctc tgcgccaatt tgcagccccg ctgcgaggtg ctggaggata tcccgccgca
     4081 gctggacttc tttgacgccg tgctgccgat cgatgccgag atgtaccgca acaagaagca
     4141 gaaaacgacg cccgagtccg ccaaagaggc cctgaccgcc ctgctgccgg tgctggaagc
     4201 ccagaccgac tggacccgcg atgcgatctt tgaggcctgc aagacgctgg ccgagcagat
     4261 ggagaagaag aacggctggc tgctgttccc gttgggcatt gccctgtccg gcaaggcccg
     4321 caccccgggc ggcggcaccg atttggccgc catgatgggc aaggcagaaa ccctaaaacg
     4381 cgtgaaggac gcactgggaa agctgtaaat gaacagtagt ttccccttgc cccggcgatg
     4441 caataaaaaa gaggagttat acccatgcgc cgggtataac tccttttttt aaatcacata
     4501 atcattcccc ggctgggcgg tgttgaccag gtcggctagg gtgatgccgt cgaagtagtc
     4561 gttgatcatc gtgttcagct tgcgccacat gggtagggtg cggcattcca ccatgcgggg
     4621 gcactccacc gggttctgtc ctaaacacgc caccggggcc aggctccctt cggtcaggcg
     4681 taaaatattc cccaccgtgt attccccggg cgttttggtc aggcggtagc cgccggactt
     4741 gccccgcagg ccggtcagat aacgcgctct gaccagactt ttgacgatgg cctccaggta
     4801 tttttccgaa atttcctgcc gagcggcaat ttcttttagg gggatgtagc cgtcgtgctg
     4861 gttctcggca atgtcgatca tgacccgcaa cgcataacgg cctttcgttg aaatcatcat
     4921 ttggtttttg ctccttcgtt ttcttatttc ctattataca agttggtaaa taggatagca
     4981 acccctttgc cgcaaaaaaa gttgccaaaa ttccctattt ccctattgac aaatgggaaa
     5041 atatgttgta caatcccatt aacctataag aaaggtatga attgatatgc gaaagggatt
     5101 tgcaccatac gcgcgatgtc gccgtcacac gctgtaaaag ccgtaacatt gtaaataaac
     5161 agaaatgacg atggaggaaa ctaccatgag caagatttat acttccgctg accagctgat
     5221 cggccgcacc cccctgctgg aactcaccca cattgaagcc gccgaaggac tggacgccaa
     5281 gatcctggcc aagctggaat atttcaaccc cgccggttcc gtcaaggacc gcattgccaa
     5341 ggcgatgatc gatgacgccg aggccaccgg caaactgaag cccggttccg tcattattga
     5401 gccgacctcc ggcaacaccg gcatcggcct ggcatccgtc gccgccgccc gcggctaccg
     5461 catcatcatc gtcatgcctg agacgatgag cgtggagcgc cgccagctga tgaaggccta
     5521 cggtgccgag ctggtgctga cagacggttc caagggcatg aagggtgcta tcgccaaggc
     5581 tgacgagctg gcgaaggaga ttcccaacag ctttgtgccc ggccagtttg tgaacccggc
     5641 caatcccgac gcccacaagc gcaccaccgg ccccgagatc tgggaggaca ccgacggcaa
     5701 ggtcgatatc tttgttgcag gtgtcggcac cggcggcacc gtgaccggcg ttggcgagta
     5761 cctgaagagc cagaaccccg ccgtcaaggt cgtggccgtc gagcctgcca cctcccctgt
     5821 gctgagcaag ggtaccgccg gtgcgcacaa gattcagggc atcggtgccg gctttgtacc
     5881 tgacgtgctg aacaccaagg tttacgacga ggtcatcccc gttgccaacg aggacgcctt
     5941 tgccaccggc aagctggtcg gccacaagga gggcgtgctg gtaggtattt cctccggcgc
     6001 tgcgctgtgg gctgccatcc agctggccaa gcgccccgag aacaagggca agaccattgt
     6061 ggccctgctg cccgacaccg gcgaccgcta tctgtccacc ccgctgttcg cggactgatt
     6121 gcaataacag gaaacctttg ctataataag ggtactgttg catacaaagt ggaggccgta
     6181 ggggccggac aggttcgacc ctcagctttc ccggcaatgc cctgtcaagg caaggccgcg
     6241 ggcggagtat gccccgcccc tacaactcaa caattttcaa tgtgcagcac ggttttgtgc
     6301 tgcacatttt ttatacaagg aggttttcca tgactactac ccccggtgcg ctgattgcga
     6361 tgagcggcgg cgtggacagc acggttgccg cttacctgat gaaaaaggcg ggttaccgct
     6421 gcatgggcgc gaccatgcgg ctgtaccaga atgaagattt gggccagagc ggcttccaca
     6481 cctgctgctc tgccaaggat gtggaggatg ccgccgatgt ggcattccag ctggacattc
     6541 ccttccaggt cgtgaactat actgaggaat ttcgcgagaa ggtcatcggc aagttcattg
     6601 ccacctacga ggcgggcggc acgccgaacc cctgcatcga ctgcaaccgc acgatgaagt
     6661 ttgacaagat gctggacttt gcgcgggaga acggcctgga ttacgttgtg accggccact
     6721 atgcccgcat agagcaagac cccgacaccg gccgctggct gctgaaaaag gccctgtacg
     6781 ccgacaagga ccagagctac gttctgtacg tgctgacgca ggatcagctg gcccacacca
     6841 agttcccgct gggcggcatg gacaagccct ccatccgcaa aattgccgag gagcagggct
     6901 tttgcaacgc ccgcaagcat gacagccagg atatctgctt tgtacccgat ggcgattacg
     6961 tcggctttat ggagcgctac accggcaaat attacccgga cggcgatttt attgacagga
     7021 acggcaaggt cgtgggccgc caccacggcg cggtgcgcta caccaacggc cagcgcaagg
     7081 gtctggggct ggcgctgggt gcaccggtct acgtctgcgg caaggacatg gagaagaaca
     7141 ctgtcaccgt cggccccgag agcgcgctgt tcaccacaac gctgatcgcc gacgatttca
     7201 actggatctc gattcccgag ctgaccgcgc cgatgcgggt aaaggccaag gcccgctacc
     7261 gccaggtgga gcagcctgcc acggtctacc cgctggacaa cggcctggtc aaggtcgtct
     7321 ttgacgaacc ccagcgcgcc atcacccgcg gccagtcagt ggtactgtat gacggggaca
     7381 ttg
//
LOCUS       DBMDSQ010000070         7376 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_2674_length_7376_cov_1.686873, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000070 DBMDSQ010000000
VERSION     DBMDSQ010000070.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 7376)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7376)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7376
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2674_length_7376_cov_1.686873"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..2869)
                     /locus_tag="ACZPAX_03905"
     CDS             complement(<1..2869)
                     /locus_tag="ACZPAX_03905"
                     /inference="COORDINATES: protein motif:HMM:NF014026.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HIR1113073.1"
                     /translation="MAKNKKKGGGVVLWSVLTGVSAVAFGAACVGTNLANASAQAVNI
                     ALKTSTNKTVGKDDSVKYFETGFDSVEDLEAHDKEIAEQLTGEGAVLLKNNGALPLAA
                     GSKISTLSHSSVGIATCGTGSADIDTSKAPTLKEALEADGLEVNPTLWDFYLTGEGSK
                     FPRNPSKDTDATGINVRSDYHVNEVPMDVYTDAVKDSFASYGDAAIITITRLAGEMYD
                     VPIDGFTDGTDALQLTQEEKDLLAMARENFDKVIVLINSTNAMQCDFLTDDSYGVDAA
                     LWIGYTGTWGLNAVADILTGEVNPSGHLVDTYCNDNSTAPSLVNFYGTQYANATEDNN
                     AEWYDFMLDGNKYYNVYSEGIYIGYRYYETRYEDAVLGQGNAGDYDYNANVAYPFGYG
                     LSYTTFDWSNFKSSYDAATDSFNVSVDVKNTGSVAGKEVVQVYFQSPYTDYDKQNKVE
                     KAAVELCGFGKTEILEPGASETVTVNVPRSELAAYDANGAKTYILDAGDYYLTAAHDS
                     HDAINNVLAAKGYTVENGMTADGNADMAWNYNVASLDKDTYSVSAVTGEAITNQFDNA
                     DPSYYGMDGINYLSRSDWQGTWPQVVTLEANEALIADLNMTGNYTADPDSDAVMPTMG
                     ADNGMTLGMMIGKSYDDPDWDKLLDQVTFDEMAKLIGQGYHNTAMVQSVSKPSTLDDN
                     GPQGFTQSLTGISTNHCAYSDENIMAATFNTELMEEVGKCIGNDVMDLGASGLYGPAM
                     DTHRNAYCGRNFEYYSEDGFLSGKIAAAEISGIQSKGVYVYMKHFALNDSETKCRCIS
                     TWANEQSIREIYLKPFEMSVTEGGAKAVMNAFARVGAVWSGADAGLMTNVLRNEWGFD
                     GFVLTDFSGNPMFTARGIALRTFDAAYGVLAGTDSWDSSDVQWTTELTTQYKDCPEVV
                     QAMRQSTHRILYVIANSCAMNGFTADTKIVKVTPWWQTALIV"
     gene            complement(2932..4851)
                     /locus_tag="ACZPAX_03910"
     CDS             complement(2932..4851)
                     /locus_tag="ACZPAX_03910"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007045642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 protein"
                     /protein_id="HIR1113074.1"
                     /translation="MRSTTKLMKNWQFTGPDGKTVAVDLPHTWNNIDGQDGGNDYWRG
                     TCTYKTQFAAPAFDVAAQQVWLQFEGVNASARVTLNGVEVASHNGGYSTFRADITTVL
                     GENNELTVEVDNSKNDRVYPQKADFTFYGGIYRDVTLLVVNKNHLALDYLGGPGVQIT
                     PTVNGSNADIHVKSWTEGDGAVEFTVYEADGSEVVSGKGSDTTVTIEHPHLWDGVRDP
                     YLYTCVVRLVLNGEVQDEVSQRFGVRSFSVDSKQGFFLNGRPYPLHGVSRHQDRKGLG
                     NAITREMHDEDMQFIKELGANTIRLAHYQHDQYFYDLCDEAGMVVWAEIPYISEHMPN
                     GRENTISQMKELIVQNYNHACIVCWGVSNEITISTKDNRDMRDNHHVLNDLCHEMDKT
                     RLTTLACYAMCGPFNPVAHITDLVSWNLYLGWYVPGLFLNDLWMDFFHLCHPNRPLGF
                     SEYGAEGMPNLHSDHPHRGDHTEEYQAIYHEYMLRCFDRHKWLWATHVWNMYDFAADA
                     RDQGGEPGMNHKGLITFDRKTKKDSFYIYKAWWSDEPFVHICSKRFVDRTQSEIEVKV
                     YSNQKNVTLYVNGEKLAEQAGEHIFKFRVKLNGETKVQAVAGDSIDDAVFRKVDAPNP
                     DYKLTKKKSTSANWV"
     gene            complement(4802..6307)
                     /locus_tag="ACZPAX_03915"
     CDS             complement(4802..6307)
                     /locus_tag="ACZPAX_03915"
                     /inference="COORDINATES: protein motif:HMM:NF025854.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HIR1113075.1"
                     /translation="MDALLTLLVCAVGLCSAELAYWLPLKRRENFALRIIINFITAVP
                     FAQVITWANSGHTKPVMLLVIYGGYFTWAMVSTRLCTLLDWPASAYCSVWIVLTSESA
                     YELWRVLIWVAQALGMRHLPLHSTPMLLGQLGFTVICCIIIRYTVARTMPEDGIYHIG
                     PRQLSSAGLLGVMFVFQFFALHTSLRVGLEPSAVVAPSLLTQLYCLTLLYLQTELFKK
                     SAMQKEMDALNLLYDRQRKQYQIARQNVQIINRKCHELKVQIADLRRLHPDEAVAQSL
                     DEAEQAARMYDANAATGNEVVDVVLTEKSLLCEAQHIRLNCVADGTCLAALEPGDLYA
                     LFSNLLDQAIDAAAQQRDADHRMIDLLVCKRQGFGVINVIGPNPAESQPDHGRRHGYE
                     LKVARRVVQKYGGSMATEPRGELFAVKSYYRPPRAECKIFHPIFVQAAQRWGGLFWGQ
                     KTEYHTRNTTFRKPIAKNLTVRYNTNTIKNNTGGRNAKHDQADEELAVHRP"
     gene            complement(6308..7015)
                     /locus_tag="ACZPAX_03920"
     CDS             complement(6308..7015)
                     /locus_tag="ACZPAX_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005942793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HIR1113076.1"
                     /translation="MAKIAVVEDNTAVREELCGFIAKYAQESGRKLDVTPFADGSQIV
                     EPYRPGFDIIFLDIEMPRLGGMPTAERIRQLDPEVVLIFVTNMAQYAIRGYEVDALDF
                     VLKPVSYYQFSTKLERALQRIQRRRGGQVALQVGGGVQLLDTDDILYLETRDRLLHYH
                     TAADTWSVRGSLQKAEKDLIAYHFARCNQCYLVNLRYVKGVQDDLVLVGEQRLEISRR
                     QRTAFLGALAAYVGGAL"
     gene            complement(7115..>7376)
                     /locus_tag="ACZPAX_03925"
     CDS             complement(7115..>7376)
                     /locus_tag="ACZPAX_03925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113077.1"
                     /translation="RYVITPGKKPDPSDESGKKKVEYLTAQVWPGPWSIEHTAEEKIE
                     TQEFDGNQAGLDAAVAWLHDRYHSEPEHWENIPSILDCEPDR"
BASE COUNT         1443 a         2080 c         2225 g         1628 t
ORIGIN      
        1 ggacgatgag ggcggtctgc caccacgggg tgaccttgac gatcttggta tcagcggtga
       61 agccgttcat cgcgcaggag ttggcgatga cgtacaggat gcggtgggtg gactggcgca
      121 tggcctggac gacctcgggg cagtccttgt actgggtggt cagttcggta gtccactgta
      181 cgtcagagga gtcccagctg tcggtaccgg ccagtacgcc gtaagcagcg tcgaaggtgc
      241 gcagggcaat gccgcgggcg gtgaacatcg ggttaccgga gaagtcggtc aggacgaagc
      301 cgtcgaagcc ccactcattg cgcagaacgt tggtcatcag gccggcatca gcaccggacc
      361 agacagcgcc cacacgggca aaagcgttca taacagcctt agcgccgccc tcggtgacgg
      421 acatctcgaa aggcttgagg tagatctcac ggatggactg ctcgtttgcc caggtggaga
      481 tgcagcggca cttggtctcg ctgtcgttca gggcgaagtg cttcatgtaa acataaacgc
      541 ccttggattg gatgccgctg atctcagcag cagcgatctt gccggagagg aagccgtcct
      601 cggagtagta ctcaaagtta cggccgcagt aagcgttgcg gtgggtatcc atggcggggc
      661 cgtagaggcc ggaagcgccc agatccataa catcgttgcc gatgcacttg ccgacttctt
      721 ccatcagttc ggtgttgaag gtagcagcca tgatgttctc atcgctgtaa gcgcagtggt
      781 tggtggagat accggtcaga gactgggtga agccctgggg gccgttatcg tccagggtgc
      841 tgggcttgga aacgctctgc accatggcgg tgttgtggta gccctggccg atcagcttag
      901 ccatctcatc aaaggtaacc tggtcgagca gcttgtccca atcggggtca tcgtagctct
      961 tgccaatcat catgcccagg gtcatgccgt tgtcggcacc catggtgggc ataacagcgt
     1021 cagaatcggg gtcagcggta tagttgccgg tcatgttcaa gtcggcaatc agagcctcgt
     1081 tggcttccag ggtaacgacc tggggccagg tgccctgcca gtcagagcgg gacaggtagt
     1141 taatgccgtc cataccgtag tagctggggt cggcattgtc gaactggttg gtgatggcct
     1201 caccggtgac agcggaaacg ctgtaggtgt ccttgtccaa gctggcaaca ttgtagttcc
     1261 aggccatatc ggcgttgccg tcggcggtca tgccgttctc aacggtgtag cccttggcgg
     1321 ccagcacgtt gttgatggca tcgtgagaat cgtgggcagc ggtcaggtag tagtcaccgg
     1381 catccaggat gtaggtcttg gcaccgttgg catcgtatgc agccagttcg ctgcggggca
     1441 cgttgacggt aacggtctcg gaagcacccg gctcgaggat ctcggtctta ccgaagccgc
     1501 acagttcaac agcggccttc tcgaccttgt tctgcttatc gtaatcggtg taggggctct
     1561 ggaagtagac ctgcacgacc tctttgcctg ccacgctgcc ggtgttcttg acatcaacgc
     1621 tgacattgaa gctgtcggta gcggcatcat agctggactt aaagttggac cagtcgaagg
     1681 tggtgtagct caggccatag ccgaagggat aagcaacatt ggcgttgtag tcgtaatcgc
     1741 cagcgttgcc ctggcccagg acagcgtcct catagcgggt ctcatagtag cggtagccga
     1801 tgtagatgcc ctcgctgtag acgttgtagt acttgttgcc gtccagcatg aagtcatacc
     1861 actcagcatt gttgtcctcg gtagcgttgg cgtactgggt gccgtagaag ttaaccagag
     1921 agggagcggt gctgttgtcg ttgcagtagg tatcgaccag gtggccggag gggttgacct
     1981 cgccggtcag gatgtcggca acggcgttca ggccccaggt gccggtgtag ccaatccaca
     2041 gggcggcgtc gacgccgtag ctgtcatcgg tcaggaaatc acattgcata gcgttggtgg
     2101 agttgatcag gacgatgacc ttatcgaagt tctcgcgggc catagccagc aggtctttct
     2161 cctcctgtgt cagttgcaga gcgtcggtgc catcggtaaa gccatcgatg ggcacatcat
     2221 acatctcgcc agccaggcgg gtgatggtga tgatggcagc atcgccatag gaggcaaagc
     2281 tgtccttgac ggcgtcggtg tagacatcca tcggaacttc gttgacatgg tagtcagagc
     2341 ggacgttgat gccggtagca tcggtatcct tggaggggtt gcgggggaac ttgctgcctt
     2401 cgccggtcag gtagaaatcc cacagggtgg ggttgacttc caggccgtcg gcttccaggg
     2461 cttccttcag ggtgggagcc ttggaggtgt cgatgtcagc agagccggtg ccgcaggtgg
     2521 cgatgccaac gctggagtgg ctcagggtag agatcttgct gccagcggcc aagggcagag
     2581 caccgttgtt cttcagcagg acagcaccct cgccggtgag ctgctcggcg atctccttgt
     2641 cgtgagcttc gagatcctcg acagagtcga agccagtctc gaaatactta acactgtcat
     2701 ccttgccaac agtcttgttg gtggaggtct tgagcgcgat gttgacagcc tgggcacttg
     2761 cattggccag gttggtgccg acacaggctg caccgaaagc cactgcagaa acgccggtca
     2821 agacgctcca caggacaacg ccgccgccct tcttcttgtt ctttgccata aatctttctc
     2881 cttctccttg ttttgtataa aacaaccgcg gagtgcagtg tgtttccaaa attacaccca
     2941 gtttgcgctg gtggactttt tcttagtcag cttgtagtcc gggttcgggg catcgacctt
     3001 gcggaaaacg gcatcgtcga tgctgtcccc tgccacggcc tgaaccttcg tctcaccgtt
     3061 cagcttgacg cggaacttga agatgtgctc ccctgcctgc tcggccagct tttcgccatt
     3121 gacatacagc gtcacgtttt tctggttcga gtagaccttg acctcgatct cactctgggt
     3181 gcgatccaca aagcgcttgg agcagatgtg cacgaacggc tcgtcactcc accacgcctt
     3241 atagatatag aagctgtcct tcttggtctt gcggtcgaag gtgatcaggc ccttgtggtt
     3301 catgcccggc tcgccgccct ggtcgcgggc atcggcagcg aaatcgtaca tattccacac
     3361 atgggtggcc cacagccatt tgtgtcggtc gaagcagcgc agcatgtact cgtggtagat
     3421 ggcctgatac tcttcggtgt gatcgccgcg gtgcgggtgg tcgctgtgca ggttgggcat
     3481 accctcggca ccgtactcac tgaagcccag cgggcggttc gggtggcaga ggtggaagaa
     3541 gtccatccac aaatcattca ggaagaggcc cggcacgtac cagcccaggt acaggttcca
     3601 gctgaccagg tcagtgatgt gggccaccgg gttgaagggg ccgcacatcg catagcaggc
     3661 cagcgtggtc aggcgggtct tgtccatctc gtggcagaga tcattcagca cgtgatggtt
     3721 gtcgcgcata tcgcggttgt ccttagtgct gatggtgatc tcgttggaga cgccccagca
     3781 gacgatgcag gcgtggttgt agttctggac gatcagttcc ttcatctggc tgatcgtgtt
     3841 ttcgcggccg ttgggcatat gctcggaaat ataggggatt tcggcccaga cgaccatacc
     3901 ggcttcatcg cagaggtcgt agaagtactg gtcgtgctgg tagtgcgcca gacggatcgt
     3961 gtttgcaccc agttccttga tgaactgcat atcctcgtcg tgcatctccc gcgttatggc
     4021 attgcccagg cccttgcggt cctgatggcg ggagacgcca tgcagcggat acggacggcc
     4081 attgaggaaa aagccctgct tggagtcaac cgagaagctg cgcacgccaa agcgctggct
     4141 gacctcgtcc tgcacctcgc cgttcagcac gaggcgaacc acgcaggtgt agagatacgg
     4201 gtcacggaca ccgtcccaca ggtgcgggtg ctcgatcgtg acggtggtgt cgctgccctt
     4261 accggagacg acctcgctgc cgtcagcctc gtaaaccgtg aactcaaccg caccgtcgcc
     4321 ctcggtccag ctcttgacat ggatgtcggc gttggagccg ttgacggtgg gggtgatctg
     4381 cacgcccggg ccgccgaggt aatccagcgc aaggtggttc ttgttgacga ccagcagcgt
     4441 cacatcgcgg taaatgccgc cgtagaaggt gaagtcagcc ttctgggggt aaacgcggtc
     4501 gtttttgctg ttgtcgacct cgacggtcag ctcattgttc tcgcccaata cggtggtgat
     4561 atccgcacgg aaggtggagt aaccgccgtt gtggctggcg acctccacgc cgttcagcgt
     4621 aactcttgcg ctggcattga caccctcgaa ttgcagccag acctgctggg cagcaacatc
     4681 aaaggcgggt gccgcgaact gcgttttata ggtgcaggtg ccgcgccaat aatcattgcc
     4741 gccgtcctga ccatcgatgt tgttccaggt gtgcggcaga tcgacggcga cggtcttgcc
     4801 gtcagggccg gtgaactgcc agttcttcat cagcttggtc gtgcttcgca ttgcgccctc
     4861 ctgtgttgtt ttttatcgta tttgtattgt agcgaactgt taaattcttc gcaataggtt
     4921 tgcgaaaagt ggtatttctg gtgtgatatt cggttttttg cccccaaaaa agcccacccc
     4981 atctttgtgc agcttgcaca aagatggggt ggaaaatttt acattcagct ctgggcgggc
     5041 ggtagtacga tttgaccgca aacaactcgc cccggggttc ggtggccatg ctgccgccgt
     5101 atttctgcac cacgcggcgg gccactttca actcatatcc atggcggcgg ccatggtcag
     5161 gctgactttc cgccgggttg ggaccaatga cattgataac gccaaagccc tgccgtttgc
     5221 agaccagcag gtcgatcata cggtggtcgg cgtcccgctg ctgggcggcg gcatcgatgg
     5281 cctgatccag caaatttgag aacagtgcgt aaaggtcgcc gggttccagc gccgccaggc
     5341 aggtgccgtc ggccacacag ttcagccgga tatgctgggc ctcgcacaat aggctcttct
     5401 cggtcaggac gacatccacg acctcgttgc cggtggcagc gttggcatcg tacatccggg
     5461 cggcctgctc ggcttcgtcc aggctttggg ccacagcttc atcggggtgc aggcggcgca
     5521 ggtcggcgat ctggaccttc agctcatggc atttgcggtt gatgatctgc acgttctgcc
     5581 gcgcgatttg gtactgcttg cgctgacggt cgtacaaaag gttcagcgcg tccatttcct
     5641 tctgcatcgc cgattttttg aaaagctcgg tctgcagata gagcagcgtc aggcaataca
     5701 gttgggtcag cagcgacggc gcaaccaccg ccgaaggctc cagcccaacg cgcagactgg
     5761 tatgcagcgc aaaaaactga aagacaaaca tcacacccag cagccccgca ctgcttaatt
     5821 gccgcgggcc aatgtgatag attccatcct ccggcatcgt gcgtgccacc gtataccgga
     5881 taatgatgca gcagataacg gtaaagccca actgtcccag cagcatcggc gtactgtgca
     5941 gcggcagatg gcgcattccc agcgcctgcg ccacccaaat cagaacacgc cacagctcgt
     6001 aggccgactc cgacgtcaac acgatccaaa cgctgcaata ggcactggcc ggccagtcca
     6061 acagggtgca gagccgggtc gataccatgg cccaggtgaa atagccccca taaatcacca
     6121 gcagcattac cggcttggtg tgaccactgt ttgcccaggt aatgacctgc gcaaacggca
     6181 ccgccgtgat aaaattgatg atgatgcgca gagcaaagtt ttcacgcctt ttcaatggca
     6241 gccagtaggc cagctctgcg ctgcaaaggc caaccgcgca gaccagcaat gtcagaagtg
     6301 catccactta cagcgcaccc ccgacatagg cggccagcgc ccccaaaaag gccgtgcgct
     6361 ggcggcggct gatttccagc cgctgctccc ctaccagcac caaatcatcc tgcacgccct
     6421 tgacgtagcg cagattgaca aggtagcact ggttgcaccg ggcaaagtgg taggcaatca
     6481 aatctttttc ggccttttgc aggctgcccc ttaccgacca ggtatcggca gcggtgtggt
     6541 agtgcaaaag acggtcacgg gtctccaaat acaaaatatc atcggtgtcc agtaattgca
     6601 ccccgccgcc gacctgcagg gccacctgcc cgccgcgccg ccgctggatg cgctgcaggg
     6661 cgcgttccag cttggtgctg aactggtagt agctgaccgg cttgagcacg aaatccagcg
     6721 cgtcgacctc gtaccctcgg atggcatact gagccatgtt ggtgacgaag atcagcacga
     6781 cctcggggtc aagctggcgg atgcgctcgg ccgtgggcat accgcccagg cggggcatct
     6841 caatatccag aaagatgatg tcaaagccgg ggcgatacgg ctccacgatc tgggagccat
     6901 ccgcgaaggg ggtcacatcc agcttgcgac cgctctcctg cgcgtacttt gcgataaacc
     6961 cgcacagttc ttcccgcacg gcggtgttat cctcgactac ggcgatcttt gccatggcgc
     7021 tgtccccttc cctttacata tatatgtatt atgacaagat ttcaccaaaa aagcaacgcc
     7081 ctttggctga aaaggtgttg cttttgctaa attttcagcg atccggctca caatccagga
     7141 tcgaggggat attctcccag tgttccggct cgctgtggta gcggtcatgc agccaagcca
     7201 ccgcggcgtc cagccccgcc tgattgccgt cgaactcctg cgtctcgatt ttttcctcgg
     7261 cagtatgttc aatgctccac ggcccgggcc agacctgggc cgtcaggtac tcgactttct
     7321 ttttgccgct ctcgtcgctg gggtcgggct ttttgcccgg cgtgatgaca tagcgc
//
LOCUS       DBMDSQ010000071         7299 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_2703_length_7299_cov_1.409558, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000071 DBMDSQ010000000
VERSION     DBMDSQ010000071.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 7299)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7299)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7299
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2703_length_7299_cov_1.409558"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..637)
                     /locus_tag="ACZPAX_03930"
     CDS             complement(<1..637)
                     /locus_tag="ACZPAX_03930"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005925794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009226 - nucleotide-sugar biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR1113078.1"
                     /translation="MKKVLVSGAGGFVGARIMAQLAGRYELRAFPKGMLAAADETAVA
                     ELVWHEQPDVILHTAAISDTGYSEKHPEESYRANVELPLWVARAAQTVGAKLVCFSSD
                     QVYTGLTDIGPFAEDTQLSPANVYGRHKLEAEQRVLDARPDAVLLRATWMYDLPGYGL
                     PIRGNFLLNLYSAALHGGTLRFSCRDYRGITYVREAVEQLTQAMELPGGVYN"
     gene            846..2177
                     /locus_tag="ACZPAX_03935"
     CDS             846..2177
                     /locus_tag="ACZPAX_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005922344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1113079.1"
                     /translation="MNDTFMKEKPIFPLLTSMALPMVISMLVNALYNIVDSLFVARIS
                     EEAMTALSLVYPVQNLINAIAVGFSVGISALISLHMGAGNKEKADTAATHGMVLSLIH
                     GVIISIVCTAIMPRFLRSFTSDESVIAMGLTYSSIAFMFAVVIMAAMSFEKIFQAVGR
                     MNVTMIGLMGGSVCNIILDPLLIFGIGPFPEMGIAGAALATGIGQLVPVVFYLIVYNV
                     RPIPVKLRRACLRADAKLDLQLYAIGVPAILNLALPSVLISFLNGLLAAFSQSYVVVL
                     GIYYKLQTFLYLPANGIVQGLRPIIGYNYGAGEYDRVKKLYRTAMGMCAAIMAAGTIL
                     CLALSGQLIGLFSSSAETIAIGTVALRIICAGFIVSTISVVVSGALEGLGMGVQSLVI
                     SLCRYVVVIMPLAWAFCRVLGANGIWNAFWVTEVITAVIALGVYHKSMKLK"
     gene            complement(2371..3564)
                     /locus_tag="ACZPAX_03940"
     CDS             complement(2371..3564)
                     /locus_tag="ACZPAX_03940"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014686.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HIR1113080.1"
                     /translation="MNFIFVSPHFPKTYWNFCDRLHRNGVNVLGIGDAPFDEIPWELK
                     NCLTEYYRVDNLGNYDEMLRAVAYFTFHYGKIDWIESNNEYWLEMDAQLRTDFNITTG
                     AQNDFIERIKYKSKMKESYIAAGVPVARHHMVSTLHEARTFIKEVGYPVIVKPDNGCG
                     AEATYKLNNFTELKKFYAEPHNVQYIMEEYINGTIVSFDGVADSHCVPLFYTSNVFPT
                     PILDIVSENGDLSYWTQKTVPAALKDAGFRTIKAFGAKSRFFHCEFFQLNEDKPGLGQ
                     KGDYVALEVNMRPAGGYTPDMINYANSVDCYQIWADMVCYDEVRNAELDGPKYFCVYA
                     GRRDCHEYKHTHAQIMAKYGSRMKMAERIPQALRLDMGDQMYTAVVRSTAERDAFIRY
                     VQEKA"
     gene            complement(3601..3774)
                     /locus_tag="ACZPAX_03945"
     CDS             complement(3601..3774)
                     /locus_tag="ACZPAX_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015563959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4250 domain-containing protein"
                     /protein_id="HIR1113081.1"
                     /translation="MLPHDPVMLLSYVNTQLRDRDASLDALCDREDADKQALCAALAE
                     IGYEYNAEQNRFV"
     gene            complement(3853..4041)
                     /gene="rpmB"
                     /locus_tag="ACZPAX_03950"
     CDS             complement(3853..4041)
                     /gene="rpmB"
                     /locus_tag="ACZPAX_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HIR1113082.1"
                     /translation="MAKCSCCGKGVTFGIKVSHSHRRSNRTWNPNVKRVKAIVDGSPK
                     YIYACTRCLRSGKVTRAV"
     gene            4256..4912
                     /locus_tag="ACZPAX_03955"
     CDS             4256..4912
                     /locus_tag="ACZPAX_03955"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HIR1113083.1"
                     /translation="MNGLLSSAVILQYLLRAIVVLIAIPFHESAHALASHLLGDDTAV
                     RAGRLSLNPMRHFDPLGALCMLVGGVGWAKPVSINPYNYKNPKVGMALSAAAGPASNF
                     LLAWVSMILYKLCWYSGAGQAVPALSMFLYYMVAMNLSLGVFNLLPVPPFDGSRIALL
                     FLPQRLYFKAMKYERYIMLAVLALVFLGLLNTPLSMLVNAMWNLMLRLTGFVELLWGY
                     "
     gene            4917..5642
                     /locus_tag="ACZPAX_03960"
     CDS             4917..5642
                     /locus_tag="ACZPAX_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005926125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="segregation and condensation protein A"
                     /protein_id="HIR1113084.1"
                     /translation="MPEVLTFKIEDFEGPLDLLLYLVGKNKMNLYDVNIMELIEQYTA
                     AIREMQQNRMEISSEFIDMAAHLVQMKSALLLPRSPEAERMKAELTGRLIEYSACKEV
                     AAQLGSRARDLYTVAREPMPLVGAAEYTRRHDPNWLVQAWFNLMGRSTRKKKPTQEKF
                     EPLVTAPFVSVASRVSHMLRGLLKGSLQKMGELFSHEESRSTNVATFLALLELIRAGR
                     VKVDDSGTLQLDHTHRRRKDKTQ"
     gene            5639..6214
                     /gene="scpB"
                     /locus_tag="ACZPAX_03965"
     CDS             5639..6214
                     /gene="scpB"
                     /locus_tag="ACZPAX_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HIR1113085.1"
                     /translation="MTERQELGSLEAMLFAHAEPVETARLADALRLDTDETAELLQKL
                     QKLYDERESGMVLLYFEPDRWQMTTRPYYGEMVKRILDTRRNAPLSPAALEVLAVIAY
                     NQPVSRSFIEQVRGVDSSSTVTKLLDKGLIEEAGRLDLPGKPVAFQVTDTFLRVFGLG
                     SLADLPPLHGEAPENSEPEEAEDDDGQLEWK"
     gene            6221..7081
                     /locus_tag="ACZPAX_03970"
     CDS             6221..7081
                     /locus_tag="ACZPAX_03970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113086.1"
                     /translation="MAILFFILRLIGWVLLTILLLLVVALLLPLGIQAAYRPGEAFAK
                     AFYGPLKFTLWPRGGTQGAQSKAKSKPTQKSPEKPDTSPPPTVIKTVEGEKASVTIKV
                     EPDKSALPAPAPRPAATAAQAEAKTTPASAPEETPPAQDSKGGLPFGISDHIDAAMQL
                     LSDDPMAFAKCMLGHTGWLGRRLLRTVRVQHLDVFWTVTADEASTTAELYGAEIAALN
                     NLLAFLQQYIRLQSDRLWLEPDFTGERTGERNISFRVSIGTGVLLWLLLRLLWRLWKD
                     PQLQPAAKNS"
     gene            7115..>7299
                     /locus_tag="ACZPAX_03975"
     CDS             7115..>7299
                     /locus_tag="ACZPAX_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113087.1"
                     /translation="MAEHPIQGLMGVTIEKIRNMVNAETIIEGFLAGTIGVGVTYALC
                     ALANSIAYSSFNVENIA"
BASE COUNT         1440 a         2091 c         2110 g         1658 t
ORIGIN      
        1 agttgtacac gccgccgggc agttccattg cctgggtcag ctgctccacg gcctcgcgca
       61 cgtaggtaat gccgcggtag tcacggcagg agaaccgcag tgtgccgccg tgcagagccg
      121 cactgtacag gttcagcaga aaatttccgc ggatcggcag gccatacccg ggcagatcgt
      181 acatccaggt tgcacgcagc agcacagcat cgggccgggc atccagcacg cgctgttcgg
      241 cttccagctt gtggcggcca tagacgttgg caggggagag ctgcgtatcc tcggcaaacg
      301 gcccaatgtc cgtcagccct gtgtatacct ggtccgagct gaaacacaca agctttgcac
      361 ccactgtctg cgcggccctg gccacccaga gcggcagctc cacattggcg cgataggatt
      421 cctccgggtg tttttcgctg tatccagtgt ccgagattgc cgctgtgtgc aaaattacgt
      481 cgggctgttc gtgccacaca agctcggcca ccgccgtttc atcggcggcg gccagcatcc
      541 ctttcgggaa agcgcgcagt tcataccggc ctgccagctg cgccataatg cgtgccccca
      601 caaagccgcc tgcaccggaa accaacactt ttttcatcag agattctcct tacaaaacta
      661 ttttgtcttt attgtaccgt ttttgcgcac ccggcgcaag catcttgaca aattcggcgg
      721 tagtttgtat aataaaggca cctaaaataa ggtgtggatt tttacagttt tgatactact
      781 tttcgccaca caatggcccg ccgggctgtt gtgtggcgtt tttgcgtgcc gaaaaggaga
      841 aacccatgaa cgataccttt atgaaagaaa aaccgatttt cccgctgctg acttcgatgg
      901 cgctgcccat ggtcatttcc atgctggtca acgcgctgta caacattgtg gacagcctct
      961 tcgttgcccg catcagtgaa gaagccatga ccgcgctctc gctggtgtac ccggtgcaga
     1021 acctcatcaa tgcaatcgcg gttggtttca gcgtcggcat cagtgcgctg atctcgctgc
     1081 acatgggtgc aggcaataag gaaaaggctg acactgccgc cacccacggc atggtgcttt
     1141 cgctgattca cggcgtcatt atctccatcg tctgcacggc catcatgccg cgctttctgc
     1201 ggtccttcac cagcgatgaa tcggtcatcg ccatgggtct gacctattcc agcatcgcct
     1261 ttatgtttgc cgttgtcatc atggcggcca tgtcgtttga gaagattttc caggccgttg
     1321 gccggatgaa cgtcacaatg atcggcctga tgggcggctc cgtctgcaac atcatccttg
     1381 acccgctgct gattttcggc atcggcccct tcccggaaat gggcattgca ggcgcggcgc
     1441 tggctaccgg catcggccag ctggtaccgg tcgtgttcta cctcatcgtt tataacgtgc
     1501 ggcccatccc ggtcaagctg cgccgcgcct gcctgcgggc cgacgccaag ctggacctgc
     1561 agttgtacgc catcggcgtg ccagctatcc tgaatttggc gctgccttcg gtgcttatct
     1621 ccttcctgaa tggactgctg gcggcgttct cccagagcta tgttgtggtg ctgggcatct
     1681 actacaaatt gcagactttc ctgtatctgc ccgccaacgg cattgtgcag ggcctgcgtc
     1741 ccatcattgg ctacaactac ggcgcagggg agtatgaccg tgtcaagaag ctctaccgca
     1801 cggccatggg gatgtgcgcg gccattatgg cggcgggcac gatactgtgc ctggcgctgt
     1861 ccgggcaact gattgggctg ttcagttcca gcgcggagac catcgccatc ggcacagtcg
     1921 cattgcgcat tatctgtgcg ggcttcatcg tctcgacgat ctcggtcgtt gtctccggtg
     1981 cgctggaggg cctaggcatg ggcgtacagt cgcttgttat ttcgctgtgc cgctatgtgg
     2041 tagtcatcat gccgctggcc tgggcgttct gccgcgtgct gggtgcaaac ggcatctgga
     2101 acgccttctg ggtgacggag gtcatcaccg ccgtgattgc gctgggcgta taccataaga
     2161 gcatgaaatt gaagtaaggt atacaaagag agcctgacca gggatggtca ggctctctct
     2221 gtataaaggt ctccccttgg gggaagcttg tcaccgaagg tgactgatgt ggggcgcact
     2281 tgctgcagca gcccatttat gcgtaatcat gggcacgcct tccctcatcc ggccctttgg
     2341 ggtcaccttc tccctcgtga gaaggctttt ttacgctttc tcctgcacgt agcggatgaa
     2401 cgcgtcgcgc tcggcggtgg agcgcacgac ggcggtgtac atctggtcgc ccatgtccag
     2461 gcgcagggcc tgggggatgc gctctgccat cttcatgcga ctgccgtact tggccatgat
     2521 ctgtgcgtgg gtgtgcttgt actcgtggca gtcgcggcgg cctgcataga cacagaaata
     2581 cttggggccg tccagctcag cgttgcgcac ctcatcatag cagaccatgt cggcccaaat
     2641 ctggtaacaa tccaccgagt tcgcataatt gatcatatcg ggcgtgtagc cgccggccgg
     2701 gcgcatattg acttccagcg caacgtaatc gcccttttgg ccaaggccgg gcttgtcctc
     2761 gttgagctgg aaaaactcgc agtggaagaa ccggctcttt gcgccgaacg ccttgatggt
     2821 gcggaagccc gcatccttca gcgcggctgg cacagttttc tgcgtccagt agctcaaatc
     2881 gccattctcg ctgacgatat ccagtatcgg ggtggggaac acattggagg tgtagaacag
     2941 cggcacgcaa tggctgtcgg ccactccgtc aaagctgacg atggtgccgt tgatatattc
     3001 ctccataata tactgcacat tatgcggctc tgcatagaac tttttcagtt cggtaaagtt
     3061 attcagcttg taggtggcct cggcaccgca gccgttgtcc ggcttgacga tgaccgggta
     3121 gccgacttcc ttaataaagg tgcgggcctc atgcagggtg ctgaccatat ggtggcgggc
     3181 caccggcaca ccggcggcaa tgtagctttc cttcatcttg ctcttgtact tgatgcgctc
     3241 gatgaagtcg ttctgtgcgc cggtcgtgat gttgaaatcc gtgcgcagct gggcatccat
     3301 ttccagccaa tattcgttgt tggactcgat ccaatcgatt ttgccgtagt ggaaggtgaa
     3361 gtaggcgaca gcacggagca tctcgtcata gttaccgagg ttgtccacgc ggtaatactc
     3421 cgtcaggcag tttttcagtt cccacggaat ctcgtcgaac ggcgcgtcgc cgatgcccag
     3481 cacattgaca ccattgcggt gcaggcggtc gcagaaattc cagtaggtct tggggaaatg
     3541 cgggctgaca aagataaaat tcatcgggtt cttcaacttc ctctctctat ttggggggta
     3601 tcacacaaat cggttctgtt cggcgttgta ctcatagccg atttcggcca gagccgcaca
     3661 caacgcctgc ttgtcggcat cctcgcggtc gcagagcgca tccagagaag cgtccctgtc
     3721 gcgcaactgg gtgttgacat agctcaatag cattacgggg tcatggggga gcatgggcga
     3781 acctccttaa aaaagcaaac caaaagagaa aaagccggtc acacggatgt gaccggcatt
     3841 ttttccttcc aatcagaccg cgcgggtaac tttaccagag cgcaggcagc gggtgcaggc
     3901 gtagatatac ttcggggaac cgtccacgat cgccttcacg cgcttgacat tggggttcca
     3961 ggtacggttg ctccgacgat gagagtgaga aaccttgata ccgaacgtga cacccttgcc
     4021 acagcaagaa catttggcca taatgcttgc acctcctttt caatagcttt aacagtatag
     4081 cgtatgatgg cagaaaatgc aagtgttttc gaagattttt tttgaatttt tttcataatt
     4141 ttgcgttttt gctcaatttt gcccccaaat attgtgtttt gccgctctgc ggggcttgtt
     4201 ttgctgccgt ttctacgata taatagacta agttttaatg gggtggtgtg tgcttttgaa
     4261 cggtttgttg tcttccgcag tgattttgca gtatctgctg cgcgccatcg ttgtgctgat
     4321 tgccatacca ttccacgagt ccgcccatgc gctggccagc cacctgctgg gcgacgacac
     4381 cgccgtgcgg gcgggtcggc tctcactgaa cccgatgcgc cactttgacc cgctgggcgc
     4441 attgtgcatg ctggtgggcg gtgtcggctg ggccaagcct gtttccatca acccttacaa
     4501 ctataagaac ccgaaagtcg gcatggcgct gtccgccgca gccgggcctg ccagcaactt
     4561 tttactggcg tgggtatcga tgatcctgta caagctctgc tggtacagcg gcgcggggca
     4621 ggctgtgccc gcgctgtcga tgttccttta ttatatggtt gccatgaacc tgagcctggg
     4681 tgtgttcaac ctgctgccgg tgccgccgtt tgacggcagc cgcattgcgc tgttgttttt
     4741 gccgcagcgg ctttacttca aggcgatgaa gtacgagcgg tatatcatgc tggccgtgct
     4801 ggcgctggta ttcctgggcc ttttgaacac gccgctctcg atgctggtca acgccatgtg
     4861 gaatttgatg ctgcgcctga caggctttgt ggaactattg tgggggtatt gagtttttgc
     4921 cggaagttct gacatttaaa attgaagatt ttgagggccc gcttgatttg ctgctgtatc
     4981 tcgtgggcaa aaataaaatg aacctctatg atgtcaacat catggagctg atcgagcagt
     5041 acaccgccgc catccgtgaa atgcagcaga accgcatgga gatttccagc gagttcatcg
     5101 atatggcggc ccatctcgta cagatgaaaa gtgcgctgct gctgccccgc agccccgagg
     5161 ccgagcggat gaaggccgag ctgaccggcc gcttgattga gtacagcgcc tgcaaggagg
     5221 tcgccgccca gctgggcagc cgtgcgcggg atttgtatac cgtcgcgcgt gagccgatgc
     5281 cgctggtggg cgcagccgag tacactcgcc gccatgaccc caactggctg gtgcaggcat
     5341 ggttcaactt aatgggccgc agcacccgca agaaaaagcc cacgcaggaa aagttcgaac
     5401 ctctggtcac agcgccgttt gtctcggtgg cgagccgggt ctcccatatg ctgcgcgggc
     5461 tgctcaaggg cagtttgcag aagatggggg agctgttcag ccacgaggag agccgcagca
     5521 ccaatgtcgc tacattcctg gcactgctgg aactgatccg cgcaggccgc gtcaaggtag
     5581 atgacagcgg cactttgcag cttgaccata cccaccgcag aagaaaggac aaaacgcaat
     5641 gacagaacga caggagttgg gctcgctgga agccatgctg tttgcccacg ccgagccggt
     5701 ggagaccgcc cgcctggccg acgccctgcg gctggacaca gacgagaccg ccgaactttt
     5761 gcagaaattg cagaagctct acgacgagcg ggaaagcggt atggtccttt tgtattttga
     5821 accggaccgc tggcaaatga cgacccgccc gtactacggc gagatggtga agcgcatttt
     5881 ggacacccgc cgcaacgcgc cgctttctcc cgctgcgctg gaagtactgg ctgtcatcgc
     5941 ctacaatcag ccggtctccc gcagctttat cgagcaggtg cgcggcgtgg attcctcgtc
     6001 caccgtcaca aagctgctgg acaaagggct gattgaagaa gcaggccgcc tggatctgcc
     6061 tggcaaaccg gtggcgtttc aggtcacgga tacctttttg cgggtgttcg gtctgggcag
     6121 cctggccgac ctgccgccgc tgcacggcga agcaccggaa aacagcgagc cggaggaagc
     6181 agaggacgac gacggccagt tggaatggaa gtgattgcct atggccattc ttttcttcat
     6241 cctgcggctg atcggctggg tattgctgac aatcctgctg cttttggtcg tcgcgctgct
     6301 gctgccactg ggcatacagg cggcgtacag gccgggggag gcattcgcca aagcctttta
     6361 cgggccgctg aaattcaccc tctggccgcg cggcggcacg cagggcgcac agtccaaagc
     6421 gaaatccaag cccacgcaga agtcgccgga aaagcccgac acatccccgc cgcccactgt
     6481 catcaagacc gtggagggcg agaaggccag cgtgaccatc aaggtcgagc cggataagtc
     6541 tgcactgcct gcccccgcac cgcggcccgc agcgacagct gcgcaagccg aggccaaaac
     6601 cacaccagcg tccgcgccag aggaaacgcc ccccgcacag gacagcaagg gtggtctgcc
     6661 gttcggcatt tctgaccaca tcgacgccgc tatgcagctg ctctccgatg acccgatggc
     6721 ctttgcaaaa tgtatgctgg ggcataccgg ttggctgggc cgccgcctgc tgcgcaccgt
     6781 gcgggtgcag catctggatg tgttctggac cgtcaccgca gacgaggcca gcaccaccgc
     6841 cgagctgtac ggcgcggaga tagcagcgct gaacaatctg ctggcgtttt tgcagcagta
     6901 catccgtttg cagagcgacc gcctctggct cgagccggat ttcaccggtg agcggacagg
     6961 ggagcgcaac atctctttcc gtgtatccat cggtaccggc gtgctgctct ggttgctttt
     7021 acggctgctt tggcggctgt ggaaagaccc gcagctgcag cccgccgcca aaaattcata
     7081 aacgattttc gtattataaa aggaggaatt tcttatggct gaacatccta tccagggcct
     7141 gatgggcgtc accatcgaaa aaatccgcaa catggtcaac gccgaaacca tcatcgaagg
     7201 cttcctcgcg ggcaccatcg gcgtgggcgt cacgtacgcg ctgtgtgcgc tcgctaactc
     7261 gatcgcctac tcttcgttca acgtggagaa cattgccca
//
LOCUS       DBMDSQ010000072         7264 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_2713_length_7264_cov_1.721346, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000072 DBMDSQ010000000
VERSION     DBMDSQ010000072.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 7264)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7264)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7264
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2713_length_7264_cov_1.721346"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1689)
                     /locus_tag="ACZPAX_03980"
     CDS             complement(<1..1689)
                     /locus_tag="ACZPAX_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005945374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HIR1113088.1"
                     /translation="MAKKQQEYGNDSIRQLKGADRVRKRPAVIFGSDGVEGCAHSIFE
                     IISNSIDEARDGHGNRINVTLFPDHVVEVEDFGRGIPVDYNKAEERWNWDLVFCELYA
                     GGKYAEGSGNYDFSLGLNGLGLCATQYASEWMTADIYRDGFHYHLDFQKGENVGGLQK
                     EPFKGKRTGSVIRWKPDTEVFTDIAVPAESFKDMLRRQAVVNDGVTLVFTDKSGADAE
                     KFEYLYEHGITDYANELVGDKALTPVYFCSGAATGRDRADQPDYQVKMNVAFAFSNTV
                     QCLEYYHNSSWLEHGGSPDRAVRTAFVSQINAWLKNKGLYKKNESAITFADVQDCLVL
                     VSSSFSTRTSYENQTKKAITNKFVAQAMTEFLKHQIEVYFIEHPDEAEKACKQVLINK
                     QSREHAEKARITIKKTMTQQMDLANRVQKFVDCRTKDASRRELYIVEGDSAMGAVKQS
                     RDSEFQAIMPIRGKILNCLKADYARIFKSEIITDLIRVMGCGVELGGSHNKDLASFNL
                     DNLRFNKVVICTDADVDGYQIRTLVLTMLYRLTPTLINKGYVYIAESPLYEINTK"
     gene            complement(1875..2762)
                     /locus_tag="ACZPAX_03985"
     CDS             complement(1875..2762)
                     /locus_tag="ACZPAX_03985"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HIR1113089.1"
                     /translation="MAVYQNGIDVSRYQGNINWGRVAAAGKQFAMVRVGSSNSGGLYV
                     DPYFLQNVNGAKAAGLRVGAYYYTYARTRAAVANELTTFLNVMEGLQLEYPVFVDVED
                     FSLTSLGRAELTSLVQYAMDILYQRKWYAGWYSYTNYINSYLNAAALRQYPLWVADYR
                     STLGYNGNYAMWQYSGSGSVNGISGACDLNRSYKDFLPEIQAGGYNNYGVSGPSMETV
                     AGQRLVVFNARCEYFYTANFNDVVGYLPLGSYCVVKQSTQKYNGYDWVIFRFRGAEYW
                     TAVIGDRNRVEPCNCNCNS"
     gene            complement(2853..3011)
                     /locus_tag="ACZPAX_03990"
     CDS             complement(2853..3011)
                     /locus_tag="ACZPAX_03990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113090.1"
                     /translation="MTSTSGIAAAVRLGIDKENPQSNHFWCKNGFAVLREAAQERGIV
                     LLAERRLA"
     gene            complement(2965..3108)
                     /locus_tag="ACZPAX_03995"
     CDS             complement(2965..3108)
                     /locus_tag="ACZPAX_03995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113091.1"
                     /translation="MKNVQIEKLSDPYTIRRLTDADVPLLYAWMLRNDQYFRYCGGGT
                     PRH"
     gene            complement(3105..3689)
                     /locus_tag="ACZPAX_04000"
     CDS             complement(3105..3689)
                     /locus_tag="ACZPAX_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015563799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HIR1113092.1"
                     /translation="MRAEYREGESFSHLDFTEETFYDCDFSDCTFIDCTFENCKLDHS
                     VLSECQFIRCTITGLKTTMSRAKFTEFQNCTLNNIDWLSLQGDGSFADPIEKLQDCRL
                     KYNTFTEMNLTKFNFAGNEIQRSMFAKCNLVSADFEKCDLLDTEFYQCDMRKANFKEA
                     GGYKVDIFGCKLQDAKFSLPEAVSLLGDLRIKLL"
     gene            complement(3689..4429)
                     /gene="ymfI"
                     /locus_tag="ACZPAX_04005"
     CDS             complement(3689..4429)
                     /gene="ymfI"
                     /locus_tag="ACZPAX_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P 5-aminopentanone reductase"
                     /protein_id="HIR1113093.1"
                     /translation="MNKNVLITGGSRGIGAAAVLAFAKAGYNVAFTYNAHPDAADAVH
                     RQAEALAPGGCFLAIRADAGDSVQVQAAVEQAQRELGGLQVLVCNAGIAQVKLFTDTT
                     DEDWRRMMAVDMDGAFYACRAVLPGMIHQKYGRILLVSSMWGQTGGSCEVAYSAAKAG
                     LIGLGKALAKEEGPSGITVNVIAPGVIDTDMMAGFTAEDRAALAEETPVERLGTADEI
                     ARTMVFLADEASGYITGQVLGVNGGLVI"
     gene            complement(4432..4893)
                     /locus_tag="ACZPAX_04010"
     CDS             complement(4432..4893)
                     /locus_tag="ACZPAX_04010"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HIR1113094.1"
                     /translation="MSDEELMQTALDEAKIAASLGEVPVGAVVAKDGEIIARAHNLRE
                     SGKNATYHAELMAIDAACKALGGWRLWQCELFVTLEPCPMCSGAIINSRLKRVVYGAR
                     DPKAGCCGGLTDLFALPFNHHPVIESGLMEAEAQALLQEFFVMLREKRKKK"
     gene            complement(4903..5814)
                     /locus_tag="ACZPAX_04015"
     CDS             complement(4903..5814)
                     /locus_tag="ACZPAX_04015"
                     /inference="COORDINATES: protein motif:HMM:NF014566.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysostaphin resistance A-like protein"
                     /protein_id="HIR1113095.1"
                     /translation="MAKSRGVRGAAGFCAVASLVYLLVRSLLYALVSTLMGLAHPGAS
                     MAKPLGFSEVSLALFQLIIGLGAIGLTLVFMMKLTRLRSDDLRLTLPAPWSPGFCLPV
                     FLGVANLANLAGALINHITGSETTAQMLPSGGPELFMYYLALCVMPAVAEELLFRGAF
                     QGLMRPSGSAAAIFAPALLFGLLHLDLAQGLTAFVCGVFLGWLAERSGSILPGMLLHL
                     VNNTLAFLTMYLRYYAPTEASFGVELFLLLFFPLFGLWMIWHARGQGFRFSAGLRPGV
                     DVLTVFTSPAYSAVVVFLVVYAVIFVH"
     gene            6192..7070
                     /locus_tag="ACZPAX_04020"
     CDS             6192..7070
                     /locus_tag="ACZPAX_04020"
                     /inference="COORDINATES: protein motif:HMM:NF015431.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR1113096.1"
                     /translation="MTLTDCQLLSIVAQLGTFAAAAERMHITPSAVSHAVSGVEAECG
                     FPLFTRTKSGVTLTAAAEKLMPAIQQMLASSESLEQSLAQINGMHKGALRLGVFNSAC
                     VTWLPKIVPGFRAAFPGIDVQIYQGSYDDITGWLKTGAVELGFLSNSSAHDLDFEPLY
                     DDRLICIAPPDYKPRRPGVVRAEELRGQPFVSQQSDVDADIQSYFKKNDLHVSSQCYI
                     VDDQSMIAMVACGQGLALMPELMFKEGAASAGCQVLQLEPAAKRSIGLACLSRGALSP
                     AAKEFVKHAREFARMR"
     gene            complement(7109..>7264)
                     /locus_tag="ACZPAX_04025"
     CDS             complement(7109..>7264)
                     /locus_tag="ACZPAX_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006574238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR1113097.1"
                     /translation="GDAVKGARTVVEAVAYSKTVAEAMHEYMQTLPPDLPDEYANVPV
                     AEYDEGN"
BASE COUNT         1460 a         2161 c         2090 g         1553 t
ORIGIN      
        1 cttcgtgttg atctcgtaca gcggcgactc ggcgatgtag acatacccct tgttgataag
       61 ggtcggcgtc agacggtaga gcatcgtcag cactagggtg cggatctgat agccgtcgac
      121 atcggcatcg gtacagatga cgactttatt aaagcgcagg ttgtccaggt tgaagctggc
      181 caggtctttg ttgtggctgc cgcccagctc aacaccgcag cccatgacgc ggatcagatc
      241 ggtgatgatc tccgacttga aaatacgggc gtagtcggct ttcaggcagt tcaggatctt
      301 gccgcggatg ggcatgatgg cctggaactc cgagtcgcgg ctctgcttga ccgcacccat
      361 agcggaatcg ccctccacga tgtacagctc gcggcggctg gcatccttgg tgcggcagtc
      421 gacgaacttc tgcacgcggt tggcgaggtc catctgctgg gtcatcgtct ttttgatggt
      481 gatgcgcgcc ttttcggcgt gttcacggct ctgcttattg ataagaacct gcttgcaggc
      541 cttttcggcc tcgtcggggt gctcaatgaa gtagacctcg atctgatgct tgaggaactc
      601 ggtcatggcc tgggccacaa atttgtttgt gatggctttc ttggtttggt tctcgtagct
      661 cgtgcgggtg gagaagctgg acgacaccag cacaaggcag tcctgcacat cggcgaaggt
      721 aatcgcactt tcgtttttct tatacaggcc cttgtttttc agccaggcgt tgatctggct
      781 gacgaaggcc gtgcgcacag cacggtcggg gctgccgccg tgctccagcc agctggagtt
      841 gtggtagtat tccagacact gcacggtgtt ggagaatgcg aacgcgacgt tcatcttgac
      901 ctgatagtcc ggctggtcag cgcggtcgcg gccggtggca gcaccgctgc agaagtatac
      961 cggcgtcaag gccttatccc ctaccaattc gttggcatag tcggtgatgc cgtgctcgta
     1021 cagatactca aacttctcgg cgtccgcgcc ggacttgtcg gtgaacacca gcgtcacacc
     1081 gtcgttgacg acggcctggc ggcgcagcat atctttgaag gattccgccg ggacagcgat
     1141 atcggtaaag acctcagtgt cgggcttcca gcggatgacc gagccggtgc gcttgccctt
     1201 gaacggctct ttctgcaagc cgcccacatt ctcgcctttt tggaagtcga ggtggtagtg
     1261 gaaaccgtcg cggtagatat cggcagtcat ccactcgctg gcgtactgcg tggcacacag
     1321 gcccaggccg ttcagaccca ggctgaaatc atagttgccg ctgccctcgg cgtatttgcc
     1381 gccggcgtac agctcacaga aaacgaggtc ccagttccag cgttcctcgg ccttgttgta
     1441 gtccaccggg atgccgcggc cgaagtcctc gacctcgacg acatgatccg gaaagagcgt
     1501 gacgttgatg cggttgccgt ggccgtcgcg ggcttcatca atggagttgg agataatttc
     1561 aaaaatggaa tgggcgcagc cctcgacacc gtcggagccg aagatgaccg ccgggcgctt
     1621 gcgcacacgg tcagcacctt tcagctggcg gatactgtca ttgccgtatt cctgttgttt
     1681 ttttgccatg cggcggttcc ccctgattta tattgaaaca gtggtagttt ttgcacatat
     1741 atttttatta aaccacagtt tggaagtgtt tgtcaaatcg acttactctc tgcagcaggt
     1801 tgtagggggc agatgcgggc atccgcccct gcaagctcag tgtaatacaa caattcgttg
     1861 ttgtcaagtt gttcttatga attgcagtta caattacacg gttccacccg gttgcggtcg
     1921 ccgatgaccg cggtccaata ctcggccccc cggaagcgga agatgaccca atcatagccg
     1981 ttatatttct gggtgctctg cttgacgacg cagtagctgc ccagcggcag atagcccact
     2041 acatcgttga aattggccgt gtagaaatac tcgcaccggg cattgaacac cacaagccgc
     2101 tggcccgcca ccgtctccat agaagggccg gagaccccat aattattgta gcctccggcc
     2161 tgtatttcgg gtaaaaagtc cttgtaacta cggttcaggt cgcacgcgcc ggatatccca
     2221 ttcacactgc cgctgcccga atattgccac atggcgtagt tgccgttgta gcctaaggtc
     2281 gagcggtaat ccgccaccca cagcggatat tggcgcagcg cggcggcgtt cagataacta
     2341 ttgatgtagt tcgtgtagct ataccatccc gcgtaccatt tgcgctggta gaggatatcc
     2401 atggcgtact gcaccaggct ggtcagctcg gcgcggccca ggctggtcag gctgaaatcc
     2461 tcgacatcca caaacaccgg gtactctaat tgcagcccct ccatgacgtt caaaaaggtc
     2521 gtcagctcgt tggcgacggc ggcgcgggtg cgggcgtagg tgtagtagta ggcccccacc
     2581 cgtaggcccg ccgccttggc cccgttgaca ttctgcaaga aatacgggtc aacatacagc
     2641 ccgccgctgt tgctggaccc cacgcgcacc atggcaaact gcttgcccgc cgccgccacc
     2701 ctgccccagt tgatattgcc ctggtagcgc gacacatcaa tgccgttctg gtatacagcc
     2761 ataaaaagcc cccctttgcg gtatagtatg caaagagggg gtggggtgtg tagtatcctg
     2821 tttccgctgc gaggtcggca gacatggggc atttacgcca gacgccgttc cgcaagcagg
     2881 acgatgccgc gctcctgtgc tgcttcccgt aacactgcaa agccgttttt gcaccagaaa
     2941 tgattggact gcgggttctc cttgtcaatg ccgaggcgta ccgccgccgc aatacctgaa
     3001 gtactggtca ttgcgcagca tccatgcgta cagcaacggc acatcggcgt ccgtcaggcg
     3061 gcggattgta tacggatcgg acagcttttc aatctgtaca ttcttcatag caatttgatc
     3121 ctcaaatctc ccagcagcga cactgcctcc ggcagtgaaa attttgcatc ctgcagcttg
     3181 cagccgaaga tatcgacttt atatccgcca gcctctttga agtttgcctt ccgcatatcg
     3241 cactggtaaa actcggtgtc caacaggtcg catttctcga aatccgcgct gacgagattg
     3301 cacttggcaa acatcgagcg ttggatctcg ttgcccgcaa agttgaattt tgtaaggttc
     3361 atctcggtga aggtgttgta tttcaggcgg cagtcctgca atttttcgat cgggtcagca
     3421 aacgagccgt ctccctgcag ggacagccag tcgatattgt tgagggtgca gttttgaaat
     3481 tctgtaaact ttgcacggga catcgtggtt ttcagtcctg taattgtaca gcggatgaac
     3541 tggcactcgc tcagcacgct gtggtccagc ttgcagtttt caaatgtgca gtcgatgaaa
     3601 gtgcagtcgg agaagtcgca gtcgtaaaag gtttcctctg taaaatcaag gtgcgaaaag
     3661 ctctctccct cgcggtattc ggcgcgcatc aaatcaccag tcctccgttg acacccagca
     3721 cctggccggt gatgtagccg ctggcctcat cggccagaaa gaccatcgtg cgtgcaatct
     3781 cgtccgctgt gcccaggcgc tccaccgggg tttcctcggc caaagcggcg cggtcctctg
     3841 cggtaaagcc cgccatcatg tcggtgtcaa tgacacccgg cgcaatgacg ttgaccgtga
     3901 tgccgctggg gccttcctct tttgccagcg ccttgcccag accgatgagg cccgccttgg
     3961 ccgcgctgta cgcgacctcg cagctgccgc cggtctggcc ccacatactg ctgaccagca
     4021 ggatgcggcc atatttctgg tggatcatgc cgggcagcac ggcacggcaa gcgtagaacg
     4081 cgccgtccat gtcaacggcc atcatcctcc gccaatcctc gtcagtggtg tcggtgaaaa
     4141 gtttgacctg cgcgatgccc gcgttgcaga cgagcacctg caggccgcct agttcccgct
     4201 gtgcctgctc cacggcggct tgcacctgaa cagagtcgcc cgcgtcggcc cggatggcaa
     4261 ggaagcaccc gccgggggcg agggcttcgg cctggcggtg cacggcgtcg gcggcgtcgg
     4321 gatgggcgtt gtaggtaaag gcgacgttat agccggcctt ggcgaacgcc agcaccgccg
     4381 ctgcgccaat gccccggctg ccgccggtga tgagtacgtt tttgttcatg gttatttttt
     4441 cttccttttt tcccgcagca tcacaaaaaa ttcctgcaat agggcctgcg cttctgcctc
     4501 catcaaaccc gattcaatga ccgggtggtg gttgaagggc agggcaaaca ggtcagtcag
     4561 gccgccgcag cagccggcct tggggtcgcg ggcgccgtag acaacccgct tcaggcggct
     4621 gtttatgatg gccccgctgc acatcgggca aggttccagc gtcacgaaca gctcacattg
     4681 ccacagccgc cagccgccca gtgccttgca ggctgcgtcg atggccataa gctcggcatg
     4741 gtaggtggca tttttgccgc tttccctcag gttgtgggcg cgggcaatga tctcgccgtc
     4801 cttggcgacg accgccccca ccggcacttc gccgagggac gcagcaattt ttgcctcgtc
     4861 cagcgcggtc tgcatcagtt cttcatcaga catagtttcc tctcaatgta caaaaatcac
     4921 agcataaacc accaggaaaa ccacaactgc gctgtatgcc gggctggtaa agaccgtcag
     4981 cacatccacg ccgggccgca atcccgccga aaagcggaac ccctgccctc gcgcgtgcca
     5041 aatcatccac agcccgaaaa gcgggaagaa aagcagcagg aacagctcga cgccgaagga
     5101 tgcctccgtc ggggcataat accgaagata catcgtcaaa aatgccagtg tgttgttcac
     5161 cagatgcagc agcatccccg gtaaaatgct gcccgagcgc tcggccagcc agcccaggaa
     5221 cacgccgcag acaaaagccg tcaacccctg cgccagatcc agatgcagca gcccaaacag
     5281 aagtgccggg gcaaagatgg ccgccgcgct gccgctaggg cgcatcaagc cctggaacgc
     5341 gccgcggaac agtagttcct cggccacagc gggcatgacg cagagcgcca gatagtacat
     5401 gaacagctcc ggcccgccgc tgggcagcat ctgggccgtt gtctcgctgc cggtgatgtg
     5461 gttgatgagc gcccctgcca ggttggcgag gttcgccacg ccaaggaaca ccggcagaca
     5521 gaagcctgga ctccacggtg cgggcagcgt aaggcgcagg tcatcggacc gcagacgggt
     5581 cagtttcatc ataaaaacca gcgtcagacc gatggccccc agcccaataa tcaactggaa
     5641 cagcgccagg ctgacctcgc tgaagcccag gggttttgcc atactggccc ccgggtgcgc
     5701 cagccccata agggtgctga ccagcgcata cagcaggctg cgcaccagaa gatataccaa
     5761 ggatgccacc gcgcagaaac ccgccgcgcc ccgcacccca cgtgattttg ccatgtgatt
     5821 ttccaccttt acgttaaagg cttccccctt gggggaagct ggccgtgaaa cggcctgatg
     5881 aggggccggt ctgccgcagc cacccattca cggtttgccg cgggaaggcc accctcatcc
     5941 gccccttcgg ggcaccttca cccttggggg aaagctttat tagtatcata ccacaatccg
     6001 caaaataaaa aaagccccac cggtgtgcgg gccggtgggg tgaggttgga gggagaggag
     6061 tcattttcaa cctgcattgt gcgatgcagg cattgacttt ctgactttat gatagtacat
     6121 tataggcatg aagtaaagct caactttttc atacatcgca tgaattttat tcaccatagg
     6181 agatgcagac catgactctg acagactgcc agctgttaag cattgtcgcc cagctgggca
     6241 cctttgccgc cgcggcggag cgtatgcaca tcaccccttc cgccgtcagc cacgcggtgt
     6301 ccggcgtgga ggccgagtgc ggctttccgc tgttcacccg caccaaaagc ggcgtcaccc
     6361 tgaccgccgc cgccgaaaaa ctgatgcccg ccatccagca aatgctggcc agcagcgagt
     6421 cgctggagca gtcccttgcc cagatcaacg gtatgcacaa gggtgcgctg cggctggggg
     6481 tgttcaactc ggcctgcgta acctggctgc ccaagatcgt gccgggcttc cgggctgcgt
     6541 tcccgggcat cgacgtgcaa atttaccagg gatcctacga tgatattacc ggctggctga
     6601 aaaccggcgc ggtggaattg ggatttctct ccaattccag cgcccatgac ctggattttg
     6661 agccgctgta cgatgaccgc ctgatctgca ttgcaccgcc ggactacaag ccccgccgcc
     6721 ccggcgtggt gcgggccgag gaactgcgcg gccagccttt tgtcagccag cagtccgacg
     6781 tggacgccga catccagagt tatttcaaaa agaacgatct gcacgtgtcg agtcaatgct
     6841 atatcgtgga cgaccagagc atgatcgcca tggtggcctg cgggcagggc ttggccctga
     6901 tgcccgagct gatgtttaaa gagggtgccg ccagcgcggg ctgccaggtt ttgcagctgg
     6961 aacccgccgc caagcgcagc attggattag cgtgtttgtc gcgtggggcg ctgtcccccg
     7021 cggcgaagga gtttgtgaag catgcaaggg agtttgcgcg gatgcgataa agccctcccc
     7081 ccatgggggg gaaaaaagcc tttttatgtt agtttccctc gtcatactcc gcgacaggta
     7141 cattggcgta ctcgtcgggc agatccgggg gcagggtctg catatactcg tgcatggcct
     7201 cggccacggt cttgctgtat gccacggcct cgaccaccgt acgtgcgccc ttgacggcgt
     7261 cgcc
//
LOCUS       DBMDSQ010000073         7214 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_2731_length_7214_cov_1.785604, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000073 DBMDSQ010000000
VERSION     DBMDSQ010000073.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 7214)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7214)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7214
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2731_length_7214_cov_1.785604"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            2..499
                     /gene="ruvC"
                     /locus_tag="ACZPAX_04030"
     CDS             2..499
                     /gene="ruvC"
                     /locus_tag="ACZPAX_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009060867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HIR1113098.1"
                     /translation="MRVLGIDPGYAIVGWGVVEYIGNRFAPIGYGAVITEKDTPFEQR
                     LVEIYEGVLDICKRYQPEALSIEKLYYQHNQTTVIGVAEARGVILLAAAQCGVPIYEY
                     TPMQVKQAITGYGKAVKKQIQEMTRMMLHLETIPKPDDTADALGMAIAHCHCSRSRLM
                     GTPVR"
     gene            530..1129
                     /gene="ruvA"
                     /locus_tag="ACZPAX_04035"
     CDS             530..1129
                     /gene="ruvA"
                     /locus_tag="ACZPAX_04035"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HIR1113099.1"
                     /translation="MIYCLTGKIIKKSLDSVVISCAGVGYFVQIPATTGEALPSAGQE
                     GTVYTIMNVTENDVSLYGFATEEQRDCFKMLTGVTGVGPKAGLSILSIMTPEKVALAA
                     SSGDHKAFTKASGVGPKLAQRIALELKDKVGKGLAAGTGFSGNIATPAPSSAPAQAVA
                     ALVSLGYSASDAAAAVARVDETLSVQEIITIALRSLSRV"
     gene            1196..2203
                     /gene="ruvB"
                     /locus_tag="ACZPAX_04040"
     CDS             1196..2203
                     /gene="ruvB"
                     /locus_tag="ACZPAX_04040"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015537225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HIR1113100.1"
                     /translation="MSAADAEEISLRPKTLDDYVGQEKAKGNLKVYLRAAQQRGEPMD
                     HILLYGPPGLGKTTLAGIVAQEMGVQIRITSGPAIEKPGDLAALLTNLQEGDILFIDE
                     IHRLSRQVEEVLYPALEDFALDIMIGKGPSAQSIRINLPRFTLVGATTRAGQLTGPLR
                     DRFGILLKLELYSPKELGRIIMRSAGILGVPITEEGALELARCARGTPRIANRMLKRA
                     RDFATVQGDGTIDGETAIAARKWMDIDELGLDELDRSLLRAIIEMYNGGPVGLDTLAA
                     VLGEESVTLYDVCEPYLMQMGMLTRTPRGRCVTRLAYEHLHMAVPKRFDEEDSGQTSM
                     F"
     gene            2233..3096
                     /locus_tag="ACZPAX_04045"
     CDS             2233..3096
                     /locus_tag="ACZPAX_04045"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015565231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR1113101.1"
                     /translation="MRPADTWRDYELLDATNHNRLERWSQTLLIRPDPQVIWKNDEAS
                     PLWAKADAVYYRSAKGGGQWNYHKKLPQKWQIHWNDLTLIVSPTGFKHTGVFPEQAVN
                     WAWYQEKIKAAGRPIRVLNLFGYTGGATLACLAAGASVTHVDASKGMVAWARENAAAS
                     NLADRPCRWIVDDCVKFVQREIRRGSRYDAIIMDPPSYGRGPGGEIWKLEDNVYDLIT
                     LTEQVLSDDPLFFAINSYTEGLSPAVMEYIVKTTLCPIQGGHTHCDEIGLPVSATGGV
                     VPCGATAIWEK"
     gene            3099..3941
                     /locus_tag="ACZPAX_04050"
     CDS             3099..3941
                     /locus_tag="ACZPAX_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005934864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HIR1113102.1"
                     /translation="MPNQDSILQAQNVRFRYDPEQPTYAVDGVSVDIRRGEFVAVLGA
                     NGCGKSTLAKHFNAILLPEAGTVLVEGMDTRNEDHLYDVRQKVAMVFQNPDNQIVATI
                     VEEDVAFAPENLGVPPEEIRTRIDEAMKLAGIYEKRDAAPYKLSGGQKQRVAIAGVIA
                     MRPDCLVLDESTAMLDPHGRAQVMKTIHQLRAAGITIVSITHYMEEAAQADRVLVMSR
                     GRIVMEGTPEQVFSQTEKLHSYHLDVPQAAELRDELVKAGIPMPENVITPEACAEELF
                     KLLK"
     gene            4171..5046
                     /locus_tag="ACZPAX_04055"
     CDS             4171..5046
                     /locus_tag="ACZPAX_04055"
                     /EC_number="3.6.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005921980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HIR1113103.1"
                     /translation="MSEIIRVENLSYIYNLGMPDATKALDDVSFTVEEGDFVGIIGST
                     GSGKSTLISHFNGLNRPTSGRVLVDGKDLWEQGADLRSFRFLVGLVMQYPEYQLFEET
                     CAKDIAYGPRNMGLDEAEIQRRIKEAAAFVGLSDELLEKSPFELSGGQKRRVAIAGVM
                     AMHPRVLVLDEPAAGLDPEGRDTILSQIKDYHKKTGITVLLVSHSMEDIAKYANRVLV
                     MHRAKIAMYDTVENVFARAQELLELGLSVPQVTQIFLKLRQMGLDIPADVYTIPYAVK
                     TIQKALAAKQAGEVQ"
     gene            5047..5865
                     /locus_tag="ACZPAX_04060"
     CDS             5047..5865
                     /locus_tag="ACZPAX_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015567299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T family protein"
                     /protein_id="HIR1113104.1"
                     /translation="MLKDITIGQHFPGHSVLHRCDPRLKLVATIAYIVVLFVAPNPLG
                     LALSIALLAALYKIAQIPGKMILKSLKPIVPIVIFTAVLNLFFVTGQGEPLVHFWVLN
                     IYIEGVKYAILLAVRVCALIAGTSLLTYTTSPIVLTDAIESLLRPLAKLHFPVHELAM
                     MMTIALRFIPTLIEETEKIMNAQKARGAMLDSGTFTQRIKALVPILIPLFISAFRRAD
                     ELAMAMECRCYHGGEGRTRLKQLKFTSEDFRCLVVIAVALVVIACTRLFVPGLA"
     gene            5978..6739
                     /gene="truA"
                     /locus_tag="ACZPAX_04065"
     CDS             5978..6739
                     /gene="truA"
                     /locus_tag="ACZPAX_04065"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HIR1113105.1"
                     /translation="MNYLLWISYKGTRYAGFQVQPNAPTVCAVLQDAMQAVLGQRPDV
                     KGCSRTDSGVHARRFALSFCYTGKVPMAKLVPALNAHLPPDIRAVDIQPVPDDFHARY
                     AAHAKTYHYYILNARVDDPFTFDTCYRVGPPLDVTAMQAAAKQFVGTHDFSALCASGS
                     SAAAHGDTVRTITDCTVIQSGSQIVISVTADGYLYNMVRILAGTLVDAGLHKRTPESI
                     PPLLESRDRRQAGPTLPAKGLFLEKVEYPRLNAVL"
     gene            6888..>7214
                     /locus_tag="ACZPAX_04070"
     CDS             6888..>7214
                     /locus_tag="ACZPAX_04070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113106.1"
                     /translation="MNKSEKERLARRQRMLAREQGQAPEAGIRGTSGDISLRVPKPVR
                     RGAPPPKTPDPAAGETAARLKSLTPLYKQRQHRRRVFFAVVIAALAVAVVVMTGTLSA
                     SLALLGD"
BASE COUNT         1428 a         2307 c         2187 g         1292 t
ORIGIN      
        1 catgcgcgtt ttaggcatcg accctgggta tgctattgtg ggctggggcg ttgtggaata
       61 catcggcaac cgctttgccc ccatcgggta cggcgcggtc atcaccgaaa aagacacgcc
      121 ttttgaacag cggctcgtcg aaatttacga gggtgtgctg gatatctgca agcgctacca
      181 gcccgaagcc ctctctatcg aaaagctgta ctaccagcac aaccaaacca ccgtcatcgg
      241 cgtggctgag gcccgcggcg tcattctgct ggcggctgcc cagtgcggcg tgccgatcta
      301 tgagtacacc cccatgcagg tcaagcaggc tatcaccggg tatggcaagg ctgtgaaaaa
      361 gcagattcag gaaatgaccc gcatgatgct ccatctggaa accatcccca agcccgacga
      421 caccgccgac gccctgggca tggcgattgc ccactgccat tgcagccgca gccgcctgat
      481 gggcacacct gtgcggtaac aaatcccccc tataaaagaa ggatcaacca tgatttactg
      541 tctgactgga aaaatcatta aaaaatcgct ggattccgtc gtcataagct gcgccggggt
      601 cggctatttt gtccagatac ccgccaccac cggcgaagcc ctgccctccg ccgggcagga
      661 gggcaccgtt tacaccatca tgaacgtgac cgagaatgac gtctcgcttt acggctttgc
      721 caccgaggaa cagcgcgact gcttcaagat gctgaccggc gtcaccggcg tcggccccaa
      781 ggccggactg tcgattttgt ccatcatgac gccggaaaaa gtcgccctcg cggcgtccag
      841 cggtgaccac aaggccttta ccaaggcgtc cggcgtcggg ccgaagctgg cccagcgcat
      901 cgccctggag ctgaaagaca aggttggcaa ggggctggct gccggtaccg gcttctccgg
      961 caatattgcg acccccgcgc cctccagcgc cccggcccag gccgtggccg ccctcgtcag
     1021 cctgggctac tcggccagcg acgccgctgc cgctgtggcc cgcgtggacg aaacgctctc
     1081 cgtgcaggag atcatcacca ttgcgctgcg cagcctgtcg cgcgtgtaac agaaggggag
     1141 tgccgaattg aaccaggaat ataccgttga ccccagccgc ctcgtcagcc ccgatatgag
     1201 cgccgccgac gccgaggaaa tcagcctgcg cccgaaaacg ttggacgatt acgtcggcca
     1261 ggagaaggcc aagggcaacc tgaaagtcta cctgcgggcg gcgcagcaac gcggcgagcc
     1321 gatggaccac atcctgctct acggcccgcc gggtctgggc aaaaccacgc tggccggcat
     1381 cgtggcacag gagatgggcg tgcagatccg cattacctcc ggccccgcca tcgaaaagcc
     1441 cggcgacctg gccgccctgc tgacgaacct gcaggagggc gacattctct tcatcgacga
     1501 gattcaccgc ctgtcccgcc aggttgagga agttttgtac ccggcgctgg aagatttcgc
     1561 gctggatatc atgatcggca aaggcccgag cgcacaaagc atccgtatca acctgccgcg
     1621 ctttacgctg gtgggtgcca cgacccgcgc gggccagctg acagggccct tgcgtgaccg
     1681 cttcggcatc ctgctcaagc tcgagctgta cagcccgaag gagctgggcc gtatcatcat
     1741 gcgcagcgcc ggcatcctgg gcgtgcccat caccgaggaa ggcgcactgg aactggcccg
     1801 ctgcgcccgc ggcacgccgc gcatcgccaa ccgtatgctc aagcgtgccc gcgactttgc
     1861 caccgtgcag ggcgacggca ccatcgacgg ggagaccgcc attgccgccc gcaaatggat
     1921 ggacattgac gagttgggcc tggacgagct ggaccgcagc ctgctccgcg ccatcatcga
     1981 aatgtacaac ggcggaccgg ttggcctgga taccctagcc gccgtgctgg gcgaggagag
     2041 cgtcacgctc tacgatgtct gcgagcccta cctgatgcag atgggcatgc tgacccgcac
     2101 cccgcgcggc cgctgcgtga cccgccttgc ctacgagcac ctgcacatgg ccgtgcccaa
     2161 gcgcttcgac gaagaggaca gcggccagac gagtatgttt taacctgttg aatagataaa
     2221 ggagatttgc ttatgcgccc tgctgatacc tggcgggatt atgagctgct ggatgccacc
     2281 aaccacaacc gattggagcg ctggagccag accctgctca ttcgtcccga cccgcaggtc
     2341 atctggaaaa acgacgaggc cagcccgctg tgggccaagg ccgatgccgt ctactaccgt
     2401 tccgccaagg gcggcggcca gtggaattac cacaaaaagc tgccccagaa atggcagatt
     2461 cattggaatg acctgaccct catcgtcagc cccaccggct tcaagcacac cggcgttttc
     2521 ccggagcagg ccgtgaactg ggcctggtac caggaaaaga tcaaggccgc cggccgcccc
     2581 atccgtgtgc tgaacctctt cggctacacg ggcggcgcga cgctggcctg cctggccgcg
     2641 ggcgcctccg tcacccatgt ggatgccagc aaaggcatgg tggcctgggc gcgtgagaat
     2701 gctgccgcca gtaacctggc tgaccgcccc tgccgctgga tcgttgacga ctgcgtcaag
     2761 ttcgtacagc gggaaatccg ccgcggcagc aggtacgacg ccatcatcat ggacccgccg
     2821 agctatggcc gcggcccggg cggcgaaatc tggaagctgg aggacaacgt ctacgacctc
     2881 atcaccctga ccgagcaggt gctgagcgac gacccgctgt ttttcgccat caactcctac
     2941 accgaagggc ttagcccggc ggtcatggag tacatcgtca agaccacgct ctgccccatc
     3001 cagggcggcc acacccactg tgatgagatc ggcctgccgg tctccgccac cggcggcgtt
     3061 gtgccctgcg gcgcaacggc tatttgggag aagtaattat gccaaatcag gactccattt
     3121 tacaggcgca gaacgtgcgc ttccgctatg acccggagca gccgacctac gccgtcgacg
     3181 gcgtcagcgt tgacatccgg cgcggtgagt tcgtggccgt gctgggcgcg aacggctgcg
     3241 gcaaatccac gctggcaaag catttcaacg cgattctgct gcccgaggcc ggaactgtcc
     3301 tcgtggaggg tatggacacc cgcaacgagg accacctcta cgatgtacgt cagaaggtcg
     3361 ccatggtgtt ccagaacccc gacaaccaga tcgtagcgac catcgttgaa gaggacgtgg
     3421 cctttgcgcc ggaaaacctc ggcgtaccgc cggaggagat ccgcacccgc atcgacgagg
     3481 ccatgaagct ggccggtatc tacgaaaaac gcgatgctgc gccgtataag ttgagcggcg
     3541 gtcagaagca gcgcgtcgcc atcgcgggcg ttatcgccat gcggcccgac tgcctggtgc
     3601 tggatgagtc caccgccatg ctggatcccc acggccgcgc ccaggtcatg aaaaccatcc
     3661 accagctgcg ggcggcgggc atcaccatcg tgtccatcac ccactatatg gaggaagccg
     3721 cccaggctga ccgcgtgctg gtcatgagcc ggggccgcat cgtaatggag ggcacgcccg
     3781 agcaggtgtt cagccagacc gagaagctgc acagttacca tctggatgta ccccaggccg
     3841 ccgagctgcg cgacgagctg gtcaaggccg gtatcccgat gccggagaac gtgattacgc
     3901 cggaggcgtg cgcggaggaa ctgtttaagc tgctgaagta acccctgcct tcccccgagg
     3961 gggaaggtgg ccgagcgaag cgaggtcgga tgaggggcgg cctcacggag atatcccgct
     4021 cacgggcaat atccgcatac ctgcccctca tcagtcagcg ggcaaaccgc tgactgcttc
     4081 ccccgagggg gaagcctttc gttcagaaac atcccacgta tgccggaacg gcaccatacc
     4141 cgggctgtat ccggcgggaa aggaatcact atgtccgaaa tcattcgtgt agaaaactta
     4201 agctacatct acaacctggg gatgcccgac gctaccaagg cgctggacga cgtgtctttc
     4261 accgtcgagg aaggcgattt tgtcggcatc atcggctcca ccggcagcgg caagtctacg
     4321 ctgatcagcc atttcaacgg cctgaaccgc cccacgtcgg gccgcgtcct tgtggacggc
     4381 aaagacctgt gggaacaggg cgctgacctg cgcagcttcc gttttctcgt cggtctggtc
     4441 atgcagtacc ctgagtacca gctgttcgag gaaacctgcg ccaaggatat cgcctacggc
     4501 ccgcgcaaca tgggccttga cgaggccgag atccagcgcc gtatcaagga ggccgccgcc
     4561 ttcgtcggtt tatcggatga gctgctggaa aaaagcccct tcgagctttc gggtgggcag
     4621 aagcgccgcg ttgcgattgc gggcgttatg gccatgcacc cccgcgtgct ggtgctggac
     4681 gaacccgccg cgggcctgga ccccgagggc cgcgacacga ttttgagcca gatcaaggac
     4741 taccacaaaa aaaccggcat cacggttttg ctggtcagcc actcgatgga agatattgcc
     4801 aagtacgcca accgcgtgct ggtcatgcac cgcgccaaaa tcgccatgta tgacacggta
     4861 gaaaacgtct ttgcccgcgc acaggaattg ctggagctgg ggctgtctgt gccgcaggtc
     4921 acgcagattt tcttaaagct gcgccagatg gggcttgaca tccccgcaga cgtctacacg
     4981 ataccctatg cggtcaagac catccaaaag gcgctggccg ccaagcaggc aggggaggtg
     5041 cagtaaatgc ttaaagatat cacgatcggt cagcattttc ccgggcattc tgtgctgcac
     5101 cgctgcgacc cgcggttaaa gctggtcgcc acgattgcct acatcgtcgt gctgtttgtt
     5161 gcgccgaatc cgctggggct ggcgctgtcc attgcgcttt tggcggcgct gtacaaaatc
     5221 gcgcagattc ccggcaagat gattttgaag agcttaaagc ccatcgtgcc catcgttatt
     5281 tttacggcgg tgctgaacct gttcttcgtc acggggcagg gggagccgct ggtacacttc
     5341 tgggttttga acatttatat tgagggtgtc aagtacgcga tcctgctggc ggtacgcgtc
     5401 tgcgcactga tcgcgggcac gagcctgttg acctacacga ccagcccgat tgtgctgacc
     5461 gatgccatcg agagcctgct gcgcccgctg gcgaaactgc attttcccgt gcatgagctg
     5521 gcgatgatga tgacgatcgc gctgcggttc atcccgactt tgatcgaaga gacggaaaaa
     5581 atcatgaacg cccagaaggc ccgcggtgcc atgctggaca gcggcacctt cacccagcgc
     5641 atcaaggcgc tggtgcccat tctgattccg ctgttcatct cggcgttccg ccgcgcggac
     5701 gagctggcca tggctatgga atgccgctgc taccacggcg gcgagggccg cacccggctg
     5761 aagcagctga aattcacgag cgaggatttc cgctgcctgg tggtaatcgc ggtggcgttg
     5821 gtagtcattg cctgcacacg gttgtttgtg ccggggttgg cgtaagattt tgccgccctg
     5881 taggggccgg atatgtccgg cccgcagctt tcccgcaagt acctgctttt cttacacgac
     5941 cgggccgggc atacccggcc cctacacagt tcttactatg aattatctac tctggataag
     6001 ctacaaaggc acccgctacg ccggtttcca ggtgcagccc aacgcaccga cggtgtgcgc
     6061 ggtcttgcag gacgccatgc aggcggtgct gggccagcgg cccgatgtaa aaggctgctc
     6121 ccgcaccgat tccggcgtcc atgcgcggcg tttcgcgctc agcttctgct atactggcaa
     6181 ggtgcccatg gcaaagctgg tgcccgcgct caacgcgcat ctgccgccgg atatccgcgc
     6241 ggtagacatc cagcctgtgc cggacgactt ccacgcgcgg tacgccgccc acgccaagac
     6301 ctaccactac tatattttaa atgcgcgggt cgatgacccc ttcaccttcg acacctgtta
     6361 ccgggtcggc ccgccgctgg acgtgaccgc catgcaggct gccgcaaaac agttcgtagg
     6421 cacgcatgat ttctcagcgc tctgtgcgtc aggttccagc gccgccgccc acggcgacac
     6481 agtgcggaca attacagatt gtactgttat acaaagtggc agccaaattg tcatttccgt
     6541 cacggcggac ggctacctgt ataatatggt gcgcatcctg gcgggcacgc tggtggacgc
     6601 cgggctgcac aagcgcacgc cggaaagcat cccgcccctg ctggaaagcc gcgaccgccg
     6661 ccaggccggg ccgacgctgc ccgcaaaggg gctatttttg gagaaggtag agtatccgag
     6721 gctgaacgcc gttttgtagg ggcggcgtcc tcgacgcccc gcgggcgcct atggaatgcg
     6781 cccccacgga ttctgtatgc aggggcgggg cgtgccccgc ccgcagcttt cccgcaagct
     6841 cctgcttgcc ctacaatgcg ataccgtaac gaaaggaggc ccgccctatg aacaaatcgg
     6901 aaaaagaacg gttggcgcgg cggcagcgga tgctggcgcg ggaacagggg caagcccccg
     6961 aagccggtat ccgcggcacc agcggggata ttagcctgcg cgtgcctaag ccggtgcggc
     7021 gcggtgcgcc cccgcccaaa acgcctgacc ccgcagcagg ggagaccgcc gcgcggctca
     7081 agtccctgac accgctctac aagcagcggc agcatcggcg gcgggtgttt ttcgctgtcg
     7141 ttatcgccgc gctggccgtg gccgtcgtgg tcatgaccgg tacgctctcc gcgtcgctgg
     7201 cgctgctggg cgat
//
LOCUS       DBMDSQ010000074         7176 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_2747_length_7176_cov_2.333569, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000074 DBMDSQ010000000
VERSION     DBMDSQ010000074.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 7176)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7176)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7176
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2747_length_7176_cov_2.333569"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..312
                     /locus_tag="ACZPAX_04075"
     CDS             <1..312
                     /locus_tag="ACZPAX_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005934547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113107.1"
                     /translation="ATVLRYLRLQEQKQLVETRVKTLDAEMQRLKGRIVAKLNISCMA
                     MGEYVTMSESRRFPHSKLAKLLHYNGSDLNSIGRRTGVCLFDYGMTPQEWRSSQHGKF
                     G"
     gene            851..2317
                     /locus_tag="ACZPAX_04080"
     CDS             851..2317
                     /locus_tag="ACZPAX_04080"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007048657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR1113108.1"
                     /translation="MNGETKRIRLDQRRAPIHEALENFRRMRVVPFDVPGHKRGRGNP
                     ELTAFLGQQCVGVDVNSMKPLDNLCHPVSVIREAEELAADAFGAAHAFLMVGGTTSSV
                     QSMVLTACKRGDEIILPRNVHRSVLNALVLCGAVPVYVNPEVDNRLGISLGMKREQVA
                     KAIAEHPNAVAVLVNNPTYYGICSDLRAIVRMAHDAGMLCLADEAHGTHFYFGGGLPV
                     SAMAAGADMASVSMHKSGGSLTQSSLLLIGPNVHAGYVRQIINLTQTTSGSYLLMSSL
                     DISRRNLALRGRQVFHQVADMAEYAREEINAVGGYYAFGKELCNGNSVFDFDTTKLSV
                     HTLDIGLAGIEVYDILRDEYDIQIEFGDIGNILAYLSIGDRPQEVERLVSALAEIKRR
                     YHTDGAGLLSQEYIDPEVAASPQEAFYAPKKSLPLRETEGMVCSEFVMCYPPGIPILA
                     PGERITAEILDYIEYAKAKGCSMTGPEDPDILRLNVLA"
     gene            2333..3190
                     /gene="speE"
                     /locus_tag="ACZPAX_04085"
     CDS             2333..3190
                     /gene="speE"
                     /locus_tag="ACZPAX_04085"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007048658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HIR1113109.1"
                     /translation="MEMWFSEFHTPDVKHSIRVNRQLYSKQSDYQRIDIFETPEFGRV
                     LTLDGNVMLTERDEFIYDEMITHVPMSVHKNAKDILVIGAGDGGVVRELTRYDRVESI
                     DLVEMDPQVIEACRAYLPGNACRMDDRRVHIYYENALKFIRKCEDEYDLIIVDSSDPF
                     GPSEGLFTREFYGSCYNALRADGIMVNQQGSPFYAEDATAMQRSHQRIASTFPISKVY
                     QAHIPTFAAGYWLFGFASKKYHPINDMDAAAWNALNMRTRYYTSRLHVGAFYLPAFLE
                     EMLREVEVR"
     gene            3190..4047
                     /gene="speB"
                     /locus_tag="ACZPAX_04090"
     CDS             3190..4047
                     /gene="speB"
                     /locus_tag="ACZPAX_04090"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019162637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HIR1113110.1"
                     /translation="MLHRNVENFIGCDSSYRTASIVLYGAPFDSTTSYRPGARFGPAA
                     MRHESYGLETYSPYQDKDLTDGNVFDSGDLELCFGSSESALVDIESRAAEILRDGKLP
                     LLLGGEHLVTLGAVRAAVRKYPDLHIVHFDAHADLRDDYLGAQLSHACVLRRCHDLIG
                     DGRIHQFCIRSGDREEFQFAARHTDMHKFDFTGLAELTDWLCQADVPVYLTIDLDCLD
                     PSAFPGTGTPEAGGVSFLQLLQAIRTVTKANIVGADVNELAPMLDQSGVSTATACKVL
                     RELLLALEK"
     gene            4085..5344
                     /locus_tag="ACZPAX_04095"
     CDS             4085..5344
                     /locus_tag="ACZPAX_04095"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005923191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0009085 - lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HIR1113111.1"
                     /translation="MSKVLVIGCGGVASVAISKCCQVSDVFTELCIASRTKSKCDALA
                     AKLTPNTKTKITTAQVDADDVQQLCDLINSYKPDLVMNIALPYQDLTIMDACLACGVN
                     YMDTANYEPENTDDPEWRAIYEKRCKEAGFSAYFDYSWQWAYKKKFEDAGLTALLGCG
                     FDPGVTQAYCAYAAKHEFDSIDTIDILDCNGGDHGYAFATNFNPEINLREVSAPGSYM
                     ENGKWVEIPPMSIKREYNFDQVGQKDMYLLHHEEIESLGKNLPDVKRIRFFMTFGQSY
                     LDHMRCLEDVGMLSTTPINYNGQEIVPIQFLKALLPDPASLGPRTKGKTNIGCIFTGK
                     KDGKDKTYYIYNICDHQECYREVGSQAISYTTGVPAMCGALMMLTGEWVKPGVYTVEE
                     FNPDPFLDALDKYGLPRSENHDPVLVD"
     gene            5344..6564
                     /locus_tag="ACZPAX_04100"
     CDS             5344..6564
                     /locus_tag="ACZPAX_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015564879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
                     /protein_id="HIR1113112.1"
                     /translation="MALYDARPPFAAIGGTVPPEFAALPTPCYLLDEAALTRNAEILG
                     SLSRRTGCRVLLAQKAFSNYDLYPLLAPHLAGTEASGLFEARLGAEEMPDREVHVFCA
                     AYRADEMNELLRYADHIVFNSPAQLAKFGPAAKAAGKSVGLRINPECSTQDGHAIYDP
                     CAPGSRLGTTRAQWDAAVAANPALPQLLDGLHFHTLCEQDADALAVTLDAVAEKFGDL
                     LPKIKWLNFGGGHHITRPGYDMATLERCIRRAQHDWDVTVYLEPGEACALNAGYLLTR
                     VLDVVKNGDTTIAILDASAACHMPDVIEMPYRPPLLGAGEPGEKPCTVRLAGPTCLAG
                     DVIGDYGFDAVSEVGDLLVFGDMAIYTTCKNNTFNGMPLPGIYARKPDGTAREIVTFG
                     YSDFKYRLGQKEKK"
     gene            6842..7168
                     /locus_tag="ACZPAX_04105"
                     /pseudo
     CDS             6842..7168
                     /locus_tag="ACZPAX_04105"
                     /inference="COORDINATES: protein motif:HMM:NF014900.6"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
BASE COUNT         1563 a         2086 c         2077 g         1450 t
ORIGIN      
        1 gccaccgtgc tgcgatacct ccgcttacag gagcaaaagc aactggtaga aacgcgggtc
       61 aaaacgctgg atgcagaaat gcagcggttg aaaggacgga tcgttgccaa actcaacatc
      121 agctgcatgg caatggggga gtacgtcacg atgtcggaat cgcggcggtt cccccacagt
      181 aagctggcaa aattactgca ttacaacggc agtgatttga actccatcgg ccgtcgcacg
      241 ggggtatgtc tgtttgacta tggcatgacc ccgcaggaat ggaggtcatc gcaacacgga
      301 aaattcgggt aaagcaaaac cccgccccgg tattctcgca aaaaatattt ttgcgtcaaa
      361 atattgacac agcaattata tcatgttata ctgtgtctcg attttgaaag acgaatcgag
      421 tttgtaagag tttttcaaag caatttacct acggaccgcc cgacaggggc gggccgggat
      481 acagacattt gatgtgtcgg tcttccggcc tatggccata cggacagccg ggtccactgc
      541 cgatcggcaa tttagattgc cttattgatt gagttaaaag ctcaaatttt ataagttggt
      601 tcctttacag ccatgaaatt gcacgttttg gcgtgcagac ttgtgaaacg gtcaataaag
      661 agtagtaaaa gtaagattgc tatatagact ctgacacgct cagccttttt gaatcacgca
      721 ggaaaatctc acggtacagc gctgggagat tcggtctgcg ccccaatatg ctgaatttta
      781 acgcaagtct gcacatctgt gcatggcctg cgttttttgt tttgcaaagg gaccgcgaac
      841 ggaggtaaaa atgaacggag aaacaaagcg catccggctg gaccagcgcc gtgcccctat
      901 ccatgaagcg cttgaaaact tccgccgcat gcgcgttgtg ccctttgatg tgccgggcca
      961 caagcgcggc cgcggcaacc cggaactgac cgcctttttg ggccagcagt gcgtcggcgt
     1021 ggatgtcaac agcatgaagc cgctggacaa tctttgccac ccggtttcgg tcatccgcga
     1081 agccgaggag ttggccgccg atgcgttcgg cgcggcccat gcgtttctga tggtgggcgg
     1141 cacgacgagt tcggtacaga gcatggtgtt gacggcctgc aagcgcgggg atgaaatcat
     1201 cctgccgcgc aacgtccacc gcagtgtgct gaacgcactg gtgctctgcg gcgcggtgcc
     1261 ggtctacgtc aacccggagg tcgacaaccg cctgggcatc agcctgggca tgaagcgaga
     1321 gcaggtggct aaggccattg ccgagcaccc caacgctgtg gccgtgctgg tgaacaaccc
     1381 cacctactac ggcatttgca gcgatttacg cgccattgtg cggatggccc acgatgccgg
     1441 gatgctctgc ctggcggacg aagcccacgg cacgcacttt tactttggcg gcgggctgcc
     1501 ggtttccgct atggcggcgg gcgcggatat ggcgtcggtc tcgatgcaca agagcggcgg
     1561 cagcttgacc cagtcaagcc tgctgctgat tggccccaac gtccacgcgg gctatgtgcg
     1621 gcagatcatt aacctgaccc agacgacctc cggcagctac ctgctgatgt cgagccttga
     1681 tatttcccgc cgcaacttgg cgctgcgcgg gcggcaggtg ttccaccaag tcgccgatat
     1741 ggccgagtac gcccgagagg agatcaacgc tgtgggcggg tactacgcct ttggcaagga
     1801 gctttgcaac ggcaactccg tttttgattt tgatacgaca aaactcagcg tgcacacgct
     1861 ggatatcggc ctggcgggca tcgaggtcta cgacatcctg cgcgacgagt acgacattca
     1921 gatcgaattc ggcgatatcg gcaacatcct tgcctatctt tccatcggtg accgcccgca
     1981 ggaggtcgag cgtcttgtca gtgcgctggc tgaaatcaag cgccgctacc acactgacgg
     2041 cgcgggcctt ttgagccaag aatacatcga tcccgaggtc gccgccagcc cgcaggaagc
     2101 attttatgcc cccaaaaaga gcctgcccct gcgcgagacc gagggtatgg tctgcagcga
     2161 gttcgttatg tgctacccgc ccggcattcc gattctggca ccgggggagc gcatcaccgc
     2221 cgaaattttg gattatatcg agtacgccaa ggccaagggc tgcagcatga ccgggccgga
     2281 agaccccgac attctgcgcc tgaacgtgct ggcctgacag ggaggtttta caatggaaat
     2341 gtggttttcg gaatttcaca cccccgatgt caagcacagc atccgggtca accgccagct
     2401 ttactccaag cagagcgatt atcaacgcat cgatattttt gagacgccgg agtttggccg
     2461 cgtgctgacg ctggacggta acgtcatgct gaccgagcgc gacgagttca tctacgacga
     2521 gatgatcacc catgtgccga tgtcggtgca caaaaacgcc aaggacattc ttgtcatcgg
     2581 cgcgggtgac ggcggcgtgg tgcgggagct gacccgctat gaccgcgtcg agagcatcga
     2641 ccttgtcgag atggaccccc aggtcatcga ggcctgccgt gcctatctgc ccggcaacgc
     2701 ctgccgcatg gacgaccgcc gcgtacatat ctactacgag aacgcgctga aattcatccg
     2761 caagtgcgag gacgagtacg acttgattat cgtggattcc tctgacccgt tcggcccatc
     2821 ggaggggctg ttcacccgcg aattttacgg cagctgctat aacgctctgc gggcggacgg
     2881 catcatggtc aaccagcagg gcagcccatt ctatgccgag gatgccaccg ccatgcagcg
     2941 cagccaccag cgcattgcca gcacctttcc catcagcaag gtgtatcagg cgcatatccc
     3001 gacctttgca gcggggtact ggctgttcgg ttttgccagc aaaaaatatc atcccatcaa
     3061 tgatatggac gccgccgcgt ggaacgcgct gaacatgcgt acccgctatt acacctctcg
     3121 gctacacgtc ggggcatttt atctacccgc ctttctggaa gaaatgctgc gggaagtgga
     3181 ggtgcgttaa tgctgcatcg caatgtagaa aatttcatcg gctgcgacag cagctaccgc
     3241 acggccagca ttgttttata cggcgcgccg tttgactcta ccaccagcta ccgccccggc
     3301 gcacgctttg gccccgcggc catgcggcac gaaagctatg gcctggaaac gtacagcccc
     3361 tatcaggaca aggatctgac cgacggcaat gtgtttgaca gcggagactt ggagctgtgc
     3421 ttcggttcgt cggagagcgc actcgtggat attgaatccc gtgcggcgga gattttgcgc
     3481 gacggcaagc tgccgctgct gctgggcggg gagcatcttg tcaccttggg tgcggtgcgt
     3541 gcggcagtgc gcaagtaccc ggatctgcac attgtgcatt ttgacgccca cgccgatctg
     3601 cgggacgatt acctcggcgc acagctcagc cacgcctgcg tgctgcgccg ctgccacgat
     3661 ttgattggtg acgggcgcat ccaccagttc tgcatccgca gcggcgatag ggaagaattc
     3721 caattcgccg cacggcacac cgatatgcac aagttcgact tcaccggtct ggccgagttg
     3781 accgactggc tgtgccaggc cgatgtgccg gtctatctga caattgattt ggactgcctt
     3841 gacccgtcgg ccttccccgg cacaggcacg cccgaagcgg gcggcgtcag ctttttgcag
     3901 cttttgcagg ccatccgcac cgtgacgaag gcaaacatcg ttggcgcgga cgtcaacgaa
     3961 ctggccccca tgcttgacca gagcggtgtt tccaccgcca cagcctgcaa ggtgttaaga
     4021 gagttgctgc tggcgttgga aaagtaaaaa tctattataa attcaaacat aaaggagttc
     4081 catcatgagc aaagttcttg ttatcggctg cggcggcgtg gcaagcgtcg ccatctccaa
     4141 gtgctgccaa gtcagcgatg ttttcaccga actgtgcatt gccagccgca cgaagtccaa
     4201 gtgcgacgcg ctggctgcca agctgacccc caacaccaaa accaaaatca ccaccgcaca
     4261 ggtcgatgcc gacgatgtgc agcagctgtg tgacctcatc aacagctata aacccgacct
     4321 cgtcatgaac attgccctgc cgtatcagga cctcaccatc atggacgcct gccttgcctg
     4381 cggcgtcaac tacatggaca ccgccaacta cgagccggaa aacaccgatg acccggaatg
     4441 gcgcgccatc tatgagaagc gctgcaagga ggccggcttc tccgcctact ttgactacag
     4501 ctggcagtgg gcctacaaga aaaagtttga ggacgccggc ctgaccgccc tgctgggctg
     4561 cggctttgac ccgggtgtga cccaggccta ctgcgcctac gctgccaagc atgaattcga
     4621 ttccatcgac acgatcgaca ttctggactg taacggtggc gaccacggct atgcctttgc
     4681 caccaacttt aaccccgaga tcaacctgcg cgaagtctct gcccccggca gctacatgga
     4741 gaacggcaag tgggtcgaga ttccacccat gagcatcaag cgcgagtaca actttgatca
     4801 ggtcggccag aaggatatgt acctgctgca tcacgaggaa attgagtcct tgggtaagaa
     4861 tctgcccgat gtcaagcgca tccgcttctt catgaccttc ggccagagct accttgacca
     4921 tatgcgctgc ttggaggacg tgggtatgct ctccaccacg cccatcaact acaacggtca
     4981 ggagatcgta ccgattcagt tcctgaaggc gctgctgccc gaccccgcca gcctcggccc
     5041 ccgcaccaag ggcaagacga acatcggctg catcttcacc ggcaaaaagg acggcaagga
     5101 caagacctac tacatctata acatctgcga ccatcaggag tgctaccgcg aggtcggcag
     5161 ccaggctatc agctacacga ccggtgtgcc tgccatgtgc ggcgccctga tgatgctgac
     5221 cggcgagtgg gtcaagccgg gcgtctacac ggtcgaggag ttcaaccccg atccattcct
     5281 cgatgcactg gacaagtacg gcctgccccg cagcgagaac catgacccgg ttctggtgga
     5341 ctgatggcac tgtacgatgc acggccgccg tttgcggcca tcggcggcac agtgccaccg
     5401 gaatttgccg ctctgcccac gccctgctac ctgctggacg aagccgccct gacccgcaac
     5461 gcggagattc tgggcagtct ttcccggcgc accgggtgca gggtactgtt agcacaaaag
     5521 gcgttcagca actacgattt gtatccgctg ttggcaccgc acctggcggg caccgaggcc
     5581 agcggcctgt ttgaggcgcg gctgggtgcg gaggaaatgc ccgataggga agtgcatgtt
     5641 ttctgtgcag cctaccgcgc ggacgagatg aacgagctgc tgcggtacgc cgaccatatc
     5701 gtgttcaact caccggcaca gctggcaaag ttcggccctg cggccaaggc ggcagggaag
     5761 agcgtggggc tgcgcatcaa ccccgagtgc tcgacccagg acggccacgc gatttacgac
     5821 ccctgcgccc ccggcagccg cctgggcacg acccgcgccc agtgggatgc cgcggtggcg
     5881 gcgaacccag cgctgccgca gctgttggat ggtctgcatt tccacacctt atgtgagcag
     5941 gacgctgacg cgctggccgt gacgctggac gcagtagcgg agaaattcgg cgatttgctg
     6001 cccaaaataa agtggctgaa ctttggcggc gggcatcaca tcacccgccc cggctacgac
     6061 atggccacac tggaacgctg catccgtcga gcccagcacg attgggacgt gacggtctat
     6121 ctcgaacccg gcgaggcctg cgccctgaac gcggggtatc tcttgacccg tgtgctggat
     6181 gtggtgaaaa acggcgatac gacgattgcg attttggacg ccagcgccgc ctgccatatg
     6241 ccggacgtca tcgagatgcc ctaccgcccg ccgttgctgg gggcgggcga acccggcgag
     6301 aagccctgta ccgtgcgctt ggccgggccg acctgcctag ccggggacgt catcggtgat
     6361 tacggctttg acgccgtatc cgaagtggga gatttgctgg tattcggtga tatggcgatt
     6421 tatacgacct gcaagaacaa caccttcaac ggtatgccgt tgcccggcat ctatgcccgc
     6481 aagcctgacg gcacagcgcg ggaaatcgtg acgtttggat atagcgattt caaatatcgg
     6541 ctggggcaaa aagagaaaaa atagaagaaa cgcgaaaggc tttgcccgat tctgtcgggc
     6601 aaagcctttc gacttttcgg cattgcgtac aaaattcaaa agaatctttt gtgttttgcg
     6661 ctaaattccg aaggccattg gcgcggtaca aataggtgtg ttactgtatc ctcaatcggc
     6721 attggccgaa tggagggaat gaaatatgct taccgcacga attgaaaaga tgcgacataa
     6781 ttatgtaaat gcaaagccgc agatttcctg tgagcgtgcc gcgctctgaa cggaatccta
     6841 taaaacgagc gagggaaagc ccgcctgcat ccgcagcgca caggcgtttt atgactgctg
     6901 cgccgggctg ggggtgcata tttttgaggg ggagctcatc gtcggggcca tcggcgaata
     6961 ccgcaagtgc ggtatcctga cgccagaatt ttcgtggcaa tgggtcgacc gcgagatgga
     7021 ccactttgtc acccgcccgc aggaccctta tattatgacg gacgaccagc gccgttttgt
     7081 gcgggaaaaa atcttccctt actggaaagg caagtcgctg gaagaagcat tccttgcgcg
     7141 gctgcctgct gaaccccggc cggtttgatc agtaca
//
LOCUS       DBMDSQ010000075         7098 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_2780_length_7098_cov_1.893240, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000075 DBMDSQ010000000
VERSION     DBMDSQ010000075.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 7098)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7098)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7098
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2780_length_7098_cov_1.893240"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..309)
                     /locus_tag="ACZPAX_04110"
     CDS             complement(<1..309)
                     /locus_tag="ACZPAX_04110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113113.1"
                     /translation="MEKPAQTPNGAPHRRRRRRPAGNGSAPQAPQPAREGKAPAQPRE
                     PRKAAPQNTAAAAPKPPREGGAPRSGRPRRGQGRPNTAAQPQQPPKQAAPAPRQPAPQ
                     A"
     gene            complement(318..1607)
                     /locus_tag="ACZPAX_04115"
     CDS             complement(318..1607)
                     /locus_tag="ACZPAX_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HIR1113114.1"
                     /translation="MSEACFTAADILLPAASVPLDPWACIAVDQFTSQPEYWQRAEAR
                     AEGRPSTLHIVLPEAYLGTPQEDARLQSIRRTMDEYRRTVLTRKVHGYVYVERTQMDG
                     TVRQGLVGAVDLDAYDYAKGSQPAIRPSESTVVERIPPRLKVRHGATLETPHVMMLAD
                     DADRTLIEPIGLVKDQLPPLYDGELMLGGGRLRGWAVEDPALIAQIDAALAKLADPAA
                     FANRWPAAKGQQPMVLAVGDGNHSLATAKAYWEELKPTLSEEQRKTHPARWCLAEVCN
                     VHSPAIEIEPIHRVVFGVAAKELYAALDAWDQQQGGSTTMSDQRLRLADAYGECAVAL
                     ANPPAPLTVGSVEAFLADYLPKHPGVTVDYIHGESAAIALAANPAKPATAILLPDFAK
                     ADLFKGVVLGGVLPRKTFSMGHAEEKRYYNECRIIGL"
     gene            complement(1691..2344)
                     /locus_tag="ACZPAX_04120"
     CDS             complement(1691..2344)
                     /locus_tag="ACZPAX_04120"
                     /inference="COORDINATES: protein motif:HMM:NF014557.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rnf-Nqr domain containing protein"
                     /protein_id="HIR1113115.1"
                     /translation="MLTASIETQQIFNGIVQFISYMVLAVFAENVVLARALGVTRLIK
                     LVPDHRAQVWDFGLPLLFVMVLSAPLGWAAHNRLFPWLRDYLPDWLPISALRPIVYLT
                     CGVAAMAVAWLLLGVLPRKLRTGCRSQLSLATCSTAILGTLLICSNQNYTILQSIAFA
                     LGSGIGYVFAVFVVREGRRRLRSKAISSIFQGLPSSMIYIGVLSLAIYALVGHTVVL"
     gene            complement(2325..3710)
                     /locus_tag="ACZPAX_04125"
     CDS             complement(2325..3710)
                     /locus_tag="ACZPAX_04125"
                     /inference="COORDINATES: protein motif:HMM:NF014557.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rnf-Nqr domain containing protein"
                     /protein_id="HIR1113116.1"
                     /translation="MQQNPNNRRRGEERNETLIIPHITKEMVETARRMAERGASRAEI
                     EQAIAAMQAGKTPTEAESAAQKKPIVQPQAAAAPARNTAAHSDTVIIPTSRPRYTSAE
                     RRAMLEEVQRRQAEREAERQKLAQEEESHFRTYRDREYGTNPPPRAAKSLADKPQPDA
                     TRTIRTSKPAADATRTVQVPQSELRAAAQKAAPAPQPVQTVQTTQQFIPESAQAPQIV
                     PQTQPKPAVPAPEAAPKRRGPVGMPRTYAEAEAALNERIRADHIWLSNPVLVRGLGMA
                     PVIGAALDGQRALMLCIAALILVTFTRVLAVAVCHLTKNRFRPVVYCYSAALLDIPTY
                     ILLYRLFGTDLSLLGIYLPIMVVEPAIVKRMEFADLEPVKDAFRHGFNNALGMCIVLL
                     IVGCLRELLATGAVFGNVILHNALLPLAALPAGGFVIVGILAAVWCAAANLYTDYKHE
                     EVRRLYADRKH"
     gene            complement(3691..4710)
                     /locus_tag="ACZPAX_04130"
     CDS             complement(3691..4710)
                     /locus_tag="ACZPAX_04130"
                     /inference="COORDINATES: protein motif:HMM:NF015100.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HIR1113117.1"
                     /translation="MPNRKKIERSKNYSYYSDLLWCTVPLIGMSWYYYGPRPLLLLLA
                     ALLTAYLCDCALAPLHGTGYQSHEPSSECFAALIVLMMPASISYPIVIAAVIVAVLVK
                     EAFGGEGHYPFHPAAVGMVVAGVSWPKNVFFYPVPGTQLPLFGEVNTTVVAGMNATLR
                     TGGLPSASTMNLLTGNVSAAVGTSAALVVMACGLFLLVRGRIKLSVVLPFFIFVIGLP
                     WLFPQLNELPAFSLPWEFIRQRVYLEKYVILSGTALFGGLFLICEPVTMPDRRMSRII
                     YGAALGIMTYVFRAFSPYECAVCCALLIVGAIPEWLDLISHRTERIRFMREEEQRLAN
                     AAKSE"
     gene            complement(4726..>4836)
                     /locus_tag="ACZPAX_04135"
     CDS             complement(4726..>4836)
                     /locus_tag="ACZPAX_04135"
                     /inference="COORDINATES: protein motif:HMM:NF004076.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HIR1113118.1"
                     /translation="ETGPLCSEAGADWLVAGSSLFNAADPAAVARVLHSK"
     assembly_gap    4837..4846
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4847..5394)
                     /gene="rpe"
                     /locus_tag="ACZPAX_04140"
     CDS             complement(<4847..5394)
                     /gene="rpe"
                     /locus_tag="ACZPAX_04140"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006873714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HIR1113119.1"
                     /translation="MTKVAASILSADLANLQNDCTRLLDAGADLMHFDVMDGHFVPNI
                     SYGAPVLKCLHAALPEVYYDVHLMISEPARYAPDFAKAGANLITFHLEAVPDAVGETI
                     DAIRATGCQVGISIRPGTPVEAVYPYLDAVDLVLVMSVEPGFGGQKFIPATPERIAAI
                     RTECDRRGLSTLIEVDGGVNVET"
     gene            complement(5531..6469)
                     /locus_tag="ACZPAX_04145"
     CDS             complement(5531..6469)
                     /locus_tag="ACZPAX_04145"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002851894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR1113120.1"
                     /translation="MDKIAVLIPCYNESKTVEKVVTDFRRVLPDATVYVYDNNSTDGT
                     AELAARAGAVVRHEYQQGKGNVMRRMFREIDAEAYILVDGDDTYPAEAAPEMVAAVTE
                     RQADMVVGDRLSSTYYTQNKRPFHNFGNDLVRFCTNHLFGGKIKDIMTGYRAFSYQFV
                     KTYPVLSRGFEIETEMTIHALQRNMQVENVVIDYRDRPEGSESKLNTYSDGFKVLGTI
                     ARLFKNYRPFLFFGILAAILAVFGIGFMVPVLGEYFQTGLVPRFPTLIVCCFVLVAAL
                     LLFISGIILSSQLAKDARDFEFQLQTVHQWENKNKS"
     gene            complement(6488..>7098)
                     /locus_tag="ACZPAX_04150"
     CDS             complement(6488..>7098)
                     /locus_tag="ACZPAX_04150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113121.1"
                     /translation="NLTGNDMTQRGFNAVRIGPAVFTSLFELPSWQGVFPFLRETDVQ
                     TNAVIRTISEKFTGGMLAATPYLWVLALPLLPAFRRCLHRRRAVAGIVYGGIAAMVVM
                     TVVDCEMAGVLYRYLMDYSPVLLVGAALCWFCAEGALSRRTAVWDATAAAALPALRVV
                     MAAAVAYTAVYRFCTLFAMEPWLQGLNPSLYYTVSRLVQFWM"
BASE COUNT         1308 a         2118 c         2446 g         1216 t
ORIGIN      
        1 ggcctgcggc gcaggctggc gcggggccgg ggcggcctgt ttgggcggct gctgtggctg
       61 ggcggcagtg ttggggcggc cctgcccacg gcgcgggcgg ccgctgcggg gggcaccgcc
      121 ctcacgcggg ggcttgggtg cagcggccgc ggtgttctgg ggcgcagcct tgcggggttc
      181 gcggggctgc gcgggggcct tgccctcccg cgcgggctgc ggggcctggg gagcgctgcc
      241 gttaccggcg ggccgacggc ggcggcgacg gtgcggcgca ccgttcgggg tttgtgcggg
      301 cttttccata attctcctta taagccgatg atgcggcatt cattataata acgtttttcc
      361 tcggcgtggc ccatgctgaa ggtcttgcgg ggcagcacgc cgcccagcac aacgcccttg
      421 aagaggtctg ccttggcaaa atccggcagc agaatggccg ttgcgggctt ggccgggttg
      481 gcagccagcg caatggcagc actctcgccg tggatgtaat cgacggtcac gccggggtgc
      541 ttgggcagat agtcggccag gaatgcctcc acgctgccca ccgtcagcgg tgcgggcggg
      601 ttcgccagcg ccacggcgca ctcgccgtag gcatcggcca ggcgcagccg ctggtcagac
      661 atcgttgtgc tgcccccctg ctgctggtcc caggcgtcca gcgcggcgta cagctccttg
      721 gcggcaacgc cgaacacaac gcggtggatg ggttcgattt caatggcggg gctgtggacg
      781 ttacagacct cggccaggca ccagcgggcc gggtgggtct tgcgctgttc ctcgctcaag
      841 gtaggcttca gttcctccca ataggctttg gcggtggcca ggctgtggtt gccgtcgccg
      901 acggccagca ccatgggctg ctgccccttg gcggcgggcc agcggttggc gaacgcggca
      961 gggtcagcca gtttggcaag ggccgcgtcg atctgcgcga tgagcgccgg atcctcgacg
     1021 gcccagccgc gcaggcggcc gccgcccagc atcagctcgc cgtcgtagag cggcggaagc
     1081 tggtctttca caaggccgat gggctcgatc agcgtgcggt cggcgtcgtc ggccagcatc
     1141 atgacgtggg gcgtttccag cgtcgcaccg tggcgcactt tcagccgcgg cgggatgcgc
     1201 tccacgacgg tgctctcgct ggggcggatg gcgggctggc tgcccttggc gtagtcgtag
     1261 gcgtcgaggt ccaccgcgcc cacaaggccc tggcggaccg tgccgtccat ctgggtgcgc
     1321 tccacataga cgtagccatg tactttccgc gtcaacacgg tgcggcggta ttcgtccatt
     1381 gtgcggcgga tgctttgcag gcgggcgtcc tcctgcgggg tgcccaagta agcctccggc
     1441 aggacgatgt gcagcgtgct gggccggccc tcggcgcggg cctcggcgcg ctgccagtac
     1501 tccggctggg aggtgaactg gtccacggca atgcaggccc acgggtccag cggaacgctg
     1561 gcggcgggca gcaggatatc agcggcggta aaacaggctt cggacatgat aacgaccccc
     1621 tgacaaatgt agatcgcccg cccttaccgg gcagcgggcg ggcaatgccc gcccctgcaa
     1681 ggaacggaca ttacaaaacg acggtatggc ccaccagggc gtagatggcc aggctcagca
     1741 cgccaatgta gatcatggaa ctgggcaggc cctggaaaat cgaggaaatc gctttgctgc
     1801 gcagacggcg gcggccttcg cgcacgacaa agacggcgaa cacgtagccg atgccgctgc
     1861 ccagcgcaaa cgcgatgctc tgtaaaatcg tgtagttctg gttggagcag atgagcagcg
     1921 tgcccaggat ggcagtgctg caggtcgcca ggctcaactg gctgcggcag ccggtgcgca
     1981 gtttgcgggg cagcacgccc agcagcagcc aggccacggc catggcggcg acgccgcagg
     2041 tcaggtagac gatggggcgc agcgccgaga tcggcagcca atcgggaaga taatcgcgca
     2101 gccatggaaa aagacggttg tgcgcggccc agcccagcgg tgcggacagc accatgacaa
     2161 agagcagcgg caggccgaaa tcccacacct gggcgcggtg gtcgggtacg agctttatca
     2221 agcgggtgac gcccagcgca cgcgccagca cgacgttctc tgcaaagacg gccagcacca
     2281 tatagctgat aaactgcacg ataccgttaa aaatctgctg tgtctcaatg cttgcggtca
     2341 gcatacaggc ggcgcacctc ctcgtgttta tagtccgtgt acaggttggc ggcggcgcac
     2401 cagacggcgg ccaggatacc tacgatgaca aacccgcccg cgggcagtgc ggccagcggc
     2461 agcagggcgt tgtgcaggat gacattgccg aacaccgcgc cggtagccag cagctcacgc
     2521 aggcagccga cgatgagcag cacgatgcac atacccagcg cattgttgaa gccgtggcgg
     2581 aacgcatcct taacgggttc caggtcggca aactccatgc gcttgacgat ggcaggctcc
     2641 acgaccatga tgggcaggta gatacccagc agcgacagat cggtgccgaa cagccggtag
     2701 agcaaaatgt acgtgggaat gtcgagaagc gcggcggaat agcagtacac caccgggcgg
     2761 aaacggtttt tcgtcaggtg gcagacggcc acggccagca cgcgggtgaa cgtgacgaga
     2821 atcagtgcgg cgatgcagag catcagcgca cgctggccgt ccaacgcggc accgatgacc
     2881 ggggccatac cgaggccgcg taccaaaacc gggttggaaa gccagatgtg gtcggcgcgg
     2941 atgcgctcgt tcaaagcggc ttcggcctcg gcgtaagtgc ggggcatccc gaccgggccg
     3001 cggcgcttcg gggcggcctc gggcgcaggc acagcgggct tgggctgcgt ttgcggcaca
     3061 atctgggggg cctgagcgct ttccgggatg aactgctgtg tggtctgcac ggtctgcacg
     3121 ggctgtggcg cgggggccgc cttctgggcg gcggcacgca attcggactg cgggacctgc
     3181 acggtgcggg tcgcgtcggc agcgggtttg ctggtgcgga tggtgcgggt cgcgtcgggc
     3241 tgcggcttgt ctgcaaggga tttcgccgcg cgcggcggtg ggtttgtgcc gtactcccgg
     3301 tcgcggtagg tgcggaaatg gctttcctcc tcctgtgcga gcttctgccg ctcggcttcg
     3361 cgctcggcct ggcggcgctg gacttcctcc agcatggcgc gacgctcggc ggacgtgtag
     3421 cgcgggcggc tcgtcgggat gatgacggtg tcgctgtggg cggccgtgtt gcgggcgggc
     3481 gcagcggcgg cctgcggctg cacgatgggc tttttctggg cggcagattc agcctcagtg
     3541 ggcgtcttgc cggcctgcat ggcggcgatg gcctgctcga tctcggcacg gctggcaccg
     3601 cgctcggcca tgcggcgggc cgtctcgacc atttccttgg tgatgtgcgg gatgatcagc
     3661 gtctcgttgc gctcctcacc ccggcggcga ttattcggat tttgctgcat tggcaaggcg
     3721 ctgttcctcc tctctcataa agcggatgcg ctcggtgcgg tggctgatca ggtcaagcca
     3781 ctcggggata gcgcccacga tcagcagggc gcagcagaca gcgcactcat aggggctgaa
     3841 cgcgcggaac acgtaggtca tgatgcccag cgccgcgccg tagatgatgc ggctcatccg
     3901 gcggtcaggc atcgtgaccg gctcacagat caagaacagc ccgccgaaca gcgccgtgcc
     3961 ggacagaata acatactttt ccaggtagac gcgctggcgg atgaactccc acggcaggct
     4021 gaaggcgggc agctcgttga gctgcgggaa cagccacggc aggccgatga caaaaataaa
     4081 gaacggcagc acgacggaaa gcttgatgcg tccgcgcacg agcaggaaca gcccgcaggc
     4141 catgaccacc agcgcggcgc tggtgccgac ggcggcggag acgttgccgg tcagcaggtt
     4201 catcgtgctg gcactgggca ggccgccggt ccgcagggtg gcgttcatgc ccgcgacgac
     4261 ggtggtgttc acctcgccaa agagtggcag ctgcgtaccc gggaccgggt agaaaaagac
     4321 atttttcggc cacgagacac cggccacgac catgccgacg gcggcggggt ggaacgggta
     4381 gtgcccctca ccgccgaagg cctccttgac cagcacggca acaatgacgg cggcgatgac
     4441 gatgggataa gagatgctgg cgggcatcat cagcacgatc agcgcggcaa agcactcgct
     4501 ggacggctca tgggactggt agccggtgcc gtgcagtggg gccagggcac agtcgcacag
     4561 gtaggcggtc agaagggccg ccagcagcag cagcaacggg cgcgggccgt agtaatacca
     4621 cgacataccg atgagcggta ccgtgcacca cagcaggtcg ctgtaatagc tgtaattctt
     4681 gctgcgttca atttttttac ggttgggcat ggggtaaaag ctcctttact tgctgtgcag
     4741 tacgcgcgct acagcggcgg ggtcggcggc gttgaaaagc gagctgccgg ccacgagcca
     4801 gtcggccccg gcttcactgc acagggggcc ggtctcnnnn nnnnnngtct caacgttgac
     4861 gccgccgtcc acctcaatca aagtggaaag accgcggcgg tcacattcgg tgcggatagc
     4921 ggcaatacgc tccggtgtgg cagggatgaa tttctgcccg ccgaagccag gttccacgct
     4981 catgaccagc acgagatcca cggcgtcgag ataggggtaa acggcctcga ccggggtgcc
     5041 ggggcggatg ctgatgccca cctggcagcc ggtggcgcgg atggcgtcaa tggtctctcc
     5101 aaccgcgtcg ggtacagcct cgaggtggaa agtgatgaga tttgcacctg ccttggcgaa
     5161 gtccggggcg tagcgtgcgg gctcgctgat catcaggtgg acgtcgtaat aaacttcagg
     5221 caaagcggcg tgcaggcatt tcagcaccgg cgcgccgtag ctgatgttgg gtacaaaatg
     5281 gccgtccatc acgtcaaagt gcatcaggtc cgcgcctgcg tccaacaggc gggtgcagtc
     5341 gttttgcagg ttggccaggt ccgccgacag gatcgatgcg gcaacttttg tcatactcta
     5401 tatactccct ttgtgtagtc cctcttatat agcagtatgt gcagtgtgca catataaaaa
     5461 tattatagcc caatccgtcc caaaaagcaa gccgcccgca gggggtgcgg gcggcaggga
     5521 tgatttggaa ttatgatttg tttttattct cccactggtg cacggtttgc agctggaact
     5581 caaaatcccg cgcgtctttg gcgagctggc tggacaggat aatgcccgag ataaacaaca
     5641 gcagcgccgc caccagcaca aagcagcaga cgatcaaagt cgggaagcgc ggcacaagcc
     5701 cggtctggaa atattcgccc agcaccggca ccataaagcc gatgccgaac accgccagaa
     5761 tggccgccaa aatgccgaag aacaggaacg gacggtaatt tttgaacagc cgtgcgatgg
     5821 tgcccagcac tttaaagccg tcagagtagg tgttcagctt gctctccgac ccttccgggc
     5881 ggtcgcggta gtcgatgacg acattctcca cctgcatatt gcgctgcagg gcgtggatgg
     5941 tcatttccgt ttcgatctca aacccgcggg acagcaccgg ataggtcttg acgaactggt
     6001 agctgaatgc gcggtagccg gtcatgatgt ccttgatctt acctccgaac agatggttcg
     6061 tgcagaagcg gaccaggtcg ttgccaaagt tgtggaacgg ccgcttattc tgggtgtagt
     6121 aagtgctgga aagccggtcg ccgacgacca tgtcggcctg gcgctcggtc acggcggcga
     6181 ccatctcggg cgcggcctcg gccggatagg tgtcgtcgcc gtcaacgaga atatacgctt
     6241 ccgcgtcgat ttctcggaac atacggcgca tgacattccc cttgccctgc tggtactcgt
     6301 ggcgaacaac ggccccggcc ctggcagcca gctcggcggt gccatcggtg gagttgttgt
     6361 catagacata gacggtggca tcgggcagta cgcggcggaa atccgtcacg accttctcga
     6421 cggtcttgga ctcattgtag caagggatca gtacggcgat tttatccata atactcctcc
     6481 gggaaattta catccagaat tgaacaaggc ggctgacagt gtaatagagc gaagggttca
     6541 gcccctgcag ccacggctcc atggcgaaca gcgtgcagaa gcggtaaacg gcggtgtatg
     6601 ccacggcggc agccatgacc acgcgcagcg caggcagtgc ggcagcggcg gtggcatccc
     6661 acacggcggt gcggcggctg agtgcgccct cggcacagaa ccagcacagc gccgccccga
     6721 cgagcagcac cgggctgtaa tccatcagat agcggtagag caccccggcc atttcgcagt
     6781 ccacaacggt cataacgacc atggcggcga tgccgccgta gacaatgccc gccacggcgc
     6841 ggcggcggtg cagacagcgg cggaacgcgg gcagcagcgg cagcgccagc acccacaggt
     6901 agggcgtggc ggccagcata ccgccggtga atttctcgct gatggtgcgg atgacggcgt
     6961 tcgtctggac gtccgtctcc cgcaggaagg gaaacacgcc ctgccagctg ggcagctcaa
     7021 acagactggt aaacacggca ggcccgatgc gcacggcatt gaagccgcgc tgggtcatat
     7081 cgttgccggt caggttgt
//
LOCUS       DBMDSQ010000076         7086 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_2786_length_7086_cov_2.594417, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000076 DBMDSQ010000000
VERSION     DBMDSQ010000076.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 7086)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7086)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7086
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2786_length_7086_cov_2.594417"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /locus_tag="ACZPAX_04155"
     CDS             complement(<1..279)
                     /locus_tag="ACZPAX_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007045700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR1113122.1"
                     /translation="MNTRYEFNMDVPPREAIRLMYVSKSRFGGDWNSVPHTHSCTEVF
                     YCVDGRGQFNVEGKMLDVAPDDMVIVNPRTLHTELSYQANPLEYIVLGI"
     gene            709..2868
                     /gene="gnpA"
                     /locus_tag="ACZPAX_04160"
     CDS             709..2868
                     /gene="gnpA"
                     /locus_tag="ACZPAX_04160"
                     /EC_number="2.4.1.211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009322033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050500 -
                     1,3-beta-galactosyl-N-acetylhexosamine phosphorylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,3-beta-galactosyl-N-acetylhexosamine
                     phosphorylase"
                     /protein_id="HIR1113123.1"
                     /translation="MEQTGRLTLPTDADVVEETLRLKNLLGADALRDCDGTEMPQELL
                     TDPAKKYATYYTTRKDNAWAEANPDEVQQEYLISDRHTARGTTLRIHLMDGFHTQQLK
                     VNTLDDPKRWWEVVDRTTGEVVPTDKWFFDEPTGEVEIETVPYHEYTVSFLAFLIWDP
                     VHMYNFLTNDWKDTPHQLTYDVRQPKTQAYVKEKLRRWCEANPHIDVVRFTTFFHQFT
                     LTFDDQKREKFVEWFGYSASVSPYILEKFEKWAGYKFRPEFIVDQGYHNTMFRVPSKE
                     FRDFIEFQQQEVCALAREMVDIVHSYGKEAMMFLGDHWIGTEPYGKYFASIGLDAVVG
                     SVGSGVTLRMISDIKGVKYTEGRLLPYFFPDVFTEGGDPIGEARTNWMKARRAILRSP
                     LDRIGYGGYLKLASNWPGFIDEIQNVVGEFRQIHENMQGTKSYVAPFKVAILNCWGGL
                     RKWMSNQVHHSIYHRETYSAEGVLECLSGMPFDVEFIDFDDIRSGIPKDIGVIINVGD
                     AYTAFSGAENWIDEKVVTAIRKFVDEGGGFIGVGEPTACQHQGRYFQLSDVMGVDREM
                     GFSLSTDKYNTCDPHHFILEDIDTAVDFGEGTSRIYAQGKHYQILAQDGEYSQLVVNE
                     YGKGHSVYFAGLPYSPQNCRILLRAIYYAAGMPEEMKHYYVTNVDTEVTVFPETKRIA
                     VINNADAEEKTDLYIKGHLIDSLTLAPREMRWVDDAE"
     gene            2950..4317
                     /locus_tag="ACZPAX_04165"
     CDS             2950..4317
                     /locus_tag="ACZPAX_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019162283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR1113124.1"
                     /translation="MKKKNAAALMAAAMAASLLVGCGGGAASEAASTADSTPAESTAE
                     STAESTAADTTADGDETLTVWAWDPNFNIYALKQAEAIYQKDHPNFKLNIEENVYSDI
                     ETKLITAATSEDYSTLPDLFLMQDYSYHKMVANFPGIYTDLTDSGLDWDKFSGGKLAD
                     STVDGHHYGLPFDNGASVMAVRSDMIENAGLTVDDFKDLTWSEFEEKAQKVVDANGVP
                     MLTSSGGSELIIEMMQSAGASPVVDGEVKIADNAALKESLTVYKDMVDKGILAEYTDW
                     DQYIASMNDGKAAGVINGCWIMSSVQAAADQSGKWAIVNMPKLDGIDGATNYANCGGA
                     SWAVSSNCKNTELAFDFLKSTFGSSVELYDDLLPNAGAISSYLPAAESDVYNQPSEFY
                     GGQTVYKDIVEFAGKVPAFDCGAYYSDVRSALTDAVTNVVQNNADIDSEMQNAQDTVE
                     FNIAG"
     gene            4439..5320
                     /locus_tag="ACZPAX_04170"
     CDS             4439..5320
                     /locus_tag="ACZPAX_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008419289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR1113125.1"
                     /translation="MKKKGLNMEQKRSLTGWAFLLPAALLIFVFCFYPMVQALILSFQ
                     KGTGSAVQPAGFANYARILKDATFQQCLFNTIFYFVIQVPIMLILALILAQLLNSPDI
                     KGKGIYRTMIFLPCATSLVSYSMIFKSLFANDGLVNRVLSTVGIPTVDWFQNAWAARW
                     VIVIALLWRWTGYNMVFYLAGLQNIDYSIYEAARIDGASPLQQFVHLTIPLLKPTILL
                     TAIMSTSGTLQLFDESVNLTAGGPGKATMTLTHYIYNISFVETPKFNYAAALSVFILV
                     VVAVLSAIQMKVGDKRD"
     gene            5313..6119
                     /locus_tag="ACZPAX_04175"
     CDS             5313..6119
                     /locus_tag="ACZPAX_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004061807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR1113126.1"
                     /translation="MTKGKKILAHAVLILASFLSVFPLYYMLCGATNTSIDVVRGKLI
                     PGTYLVENFKSLVANQNLGLAMANSFRNAILMTLITLLVCSIAGYGFEIYHDKAKDAL
                     MSVLLLAMMLPFVAIMIPLFKMMSHWGLVNSWAAFVLPSISTPFMIMLFRQASRSFPN
                     DIIEAARLDGLSEIGIFFRMFVPIMRSTYGAAMTVTFMNAWNSYLWPKIVFQSNASIT
                     MPMLVANLKSGYSVDYGMLMLGVLICTLPTAIIFLCLQKSFANGITGAVK"
     gene            6119..6583
                     /locus_tag="ACZPAX_04180"
     CDS             6119..6583
                     /locus_tag="ACZPAX_04180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113127.1"
                     /translation="MDERTYCLTPEQWRHAKACLTLAKRWGLIDDDSPEALERRRAAK
                     NAQAERAEQDGKPVYGTRRFSAPAYLQYELTRFKLDFAEPCEKIRALGICPDFTDAQT
                     RAWYDANPDLFTRYAGDSFPYEEVKQIIEKRMREEVYNSLVQDLLRGPTDRP"
     gene            6543..>7086
                     /locus_tag="ACZPAX_04185"
     CDS             6543..>7086
                     /locus_tag="ACZPAX_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019162279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate depolymerase"
                     /protein_id="HIR1113128.1"
                     /translation="MCKTYYVDPQTGRDTNDGLTPEKPLATLFAVNRLALAPGDTVLL
                     KCGSVFEKQFLHLRCCGEKNCPITIAAYGEGSAPRIDADGQGLWYQDYGCALDSPTHV
                     YRGYVSSAVLLYDAAYVTVRDLELTNRADAVIGEQYSQPDKLERTGVAVVAKDRGTRC
                     GITLQNLLIHDVHGNVYDKHM"
BASE COUNT         1592 a         2090 c         1976 g         1428 t
ORIGIN      
        1 gatgcccagc acaatatatt ccagcgggtt tgcctggtag ctcaactcgg tgtgcagggt
       61 gcggggattc acaatgacca tatcgtccgg tgcgacgtcc aacattttgc cctcgacgtt
      121 gaattgaccg cgaccgtcca cgcaatagaa cacctccgtg caggaatggg tatgcggcac
      181 tgagttccag tccccgccga agcggctctt gctcacgtac atcaggcgga tggcctcccg
      241 tgggggaacg tccatattga attcataccg tgtgttcata agcggtgcgc ctccggatga
      301 caaaatgttt acaaaaattc aaaagtgtgc ggagcaatat cgttacagaa ttaagcaaga
      361 acggaaaagt aaaaagtcgc aggccgtttc cggttccgtc cttattatat acgcatttgg
      421 ggcgcatggc aagagctttc cacaaaaaat cacgatgcaa aacagaatac cggcacgttt
      481 cgctgcaggc aggcccaaaa ccggcaagct gcacaaagag aagtcttact ttttgtgcag
      541 ctttttcttt attttctgat gttggcggcg acattcggaa ataaagcaag ataatataaa
      601 tgcgcaacaa tatctatact gttcaatgcg ggcagaaaca agtatgatgt tgtcaatgaa
      661 acgatgcctg ctgcggcaga caaaacggac agagaaggga taacgaagat ggagcagacc
      721 ggaagattga cacttcccac agacgcggat gtggtcgagg agaccctgcg gctgaaaaat
      781 ctgttgggcg cggatgcgct gcgcgactgc gacggtaccg aaatgccgca ggaattgctg
      841 acagaccccg ccaaaaagta cgctacctac tatacgaccc gcaaggacaa cgcctgggcg
      901 gaggcaaacc ccgacgaggt ccagcaggag tatcttatca gcgaccgcca cacggcgcgc
      961 ggcacgacgc tgcgcattca tctgatggac ggcttccaca cccagcagtt gaaagtcaac
     1021 acgctggatg accccaagcg ctggtgggag gtcgttgacc gcacgacggg cgaggttgtc
     1081 cccaccgaca aatggttctt cgacgagccg accggtgagg tcgagatcga aaccgtcccc
     1141 tatcacgagt acaccgtcag ctttctggcg ttcctgattt gggaccccgt acacatgtac
     1201 aacttcttga ccaacgactg gaaggacacg ccgcaccagc tgacctacga tgtgcgccag
     1261 cccaagacgc aggcctatgt aaaggaaaag ctgcgccgct ggtgcgaggc caacccccac
     1321 attgatgtgg tgcggttcac gaccttcttt caccagttca cgctgacctt tgacgaccag
     1381 aagcgagaaa agttcgtgga atggttcggt tacagcgcca gcgtcagccc ctacattctg
     1441 gaaaaatttg aaaagtgggc agggtacaag ttccgccccg agttcattgt ggatcagggc
     1501 taccacaaca cgatgttccg cgttccgagt aaggagttcc gcgacttcat cgagttccag
     1561 cagcaggagg tctgcgctct tgccagagag atggtcgaca tcgtccactc ctacggcaag
     1621 gaagcgatga tgttcctcgg cgatcactgg atcggcaccg agccctacgg caagtatttc
     1681 gcctccatcg ggctggacgc cgtggtcggc tcggtcggca gcggcgttac gctgcggatg
     1741 atctccgaca tcaagggcgt caagtacacc gaaggccgtc tgctgcccta cttcttcccc
     1801 gatgtgttca ccgagggcgg agacccgatc ggcgaggccc gcaccaactg gatgaaggcc
     1861 cgccgcgcga ttctgcgcag cccgctggac cgcatcggct acggcggtta cttaaaactc
     1921 gccagcaact ggcccggctt tatcgacgag attcaaaacg tggtcggcga gttccgccag
     1981 atccacgaaa atatgcaggg cacaaagtcc tacgttgccc cgttcaaggt ggccatcctc
     2041 aactgctggg gcggcctgcg caagtggatg agcaatcagg tgcatcactc catttaccac
     2101 cgcgagacct actccgccga gggtgttctc gaatgcctga gcggcatgcc gtttgacgtg
     2161 gaattcatcg acttcgatga catccgcagc ggcatcccca aggacatcgg cgtcatcatc
     2221 aacgtcggcg atgcgtacac agccttcagt ggtgccgaga actggatcga cgaaaaggtc
     2281 gtgaccgcca tccgcaaatt tgtggacgag ggcggcggct tcatcggcgt gggcgagccc
     2341 accgcctgcc agcatcaggg gcggtacttc cagctgtccg atgtgatggg cgtggatcgt
     2401 gagatgggct tctcgttaag caccgacaaa tacaacacct gcgatccgca ccactttatc
     2461 ctggaggata ttgacaccgc cgttgatttc ggcgagggca cgagccgcat ctacgcgcag
     2521 ggcaagcact atcagatcct ggcgcaggac ggcgagtaca gccagttggt cgtcaacgag
     2581 tacggcaagg gccacagcgt ctactttgcc gggctgccct actccccgca gaactgccgc
     2641 attttgctgc gcgccatcta ctacgccgcc ggtatgccgg aggagatgaa gcactactac
     2701 gtgaccaacg tcgataccga ggtcacggta ttccccgaaa cgaagcgcat cgcggtcatc
     2761 aacaacgcag atgccgagga aaagacagac ctgtacatca aggggcatct gatcgacagc
     2821 ctgacgctgg ccccgcgcga gatgcgctgg gtagacgatg ccgaataaca cccgataatt
     2881 tatgccccgc cgtgggacat acgttataca gccaaagaat ggagaaacat ttttaaggag
     2941 gaaatcccca tgaaaaagaa gaatgcagca gcccttatgg cggcagctat ggcagcatcc
     3001 ctgctggtcg gctgcggcgg cggtgccgcc agcgaagccg cctctaccgc tgacagcacc
     3061 cctgccgagt ccacagcgga atccaccgca gaatccactg ccgccgacac caccgctgac
     3121 ggcgacgaga ccctgaccgt atgggcatgg gaccccaact tcaacatcta cgccctgaag
     3181 caggcggagg ccatctacca gaaggaccac cccaacttca agctgaacat tgaagaaaac
     3241 gtctacagcg acatcgagac caagctcatc accgctgcca ccagcgagga ttacagcacc
     3301 ctgcctgatc ttttcctgat gcaggactac tcctaccaca agatggtcgc caacttcccc
     3361 ggtatctaca ccgacctgac cgactccggc ttggattggg acaagttctc cggcggcaag
     3421 ctggctgatt ctaccgtcga tggccaccac tacggtctgc ccttcgacaa cggcgcttcc
     3481 gtcatggcgg ttcgttccga tatgatcgag aatgccggtc tgaccgtcga cgacttcaag
     3541 gatctgacct ggagcgagtt cgaggagaag gcgcagaagg tcgtggacgc caacggcgtg
     3601 ccgatgctga cctcctccgg cggttccgag ctgatcatcg agatgatgca gtctgccggt
     3661 gcctcccccg tggtggacgg cgaggtcaag atcgccgaca atgccgcgct gaaagagtcc
     3721 ctgaccgtct acaaggacat ggtcgacaag ggcattctgg ccgagtacac cgactgggac
     3781 cagtacattg cttccatgaa tgacggcaag gctgccggcg tcatcaacgg ctgctggatc
     3841 atgtccagcg tgcaggctgc cgcagatcag tccggcaagt gggctatcgt gaatatgccc
     3901 aagctggacg gcatcgacgg tgccaccaac tacgcaaact gcggcggcgc aagctgggcc
     3961 gtttcctcca actgcaagaa caccgagctg gcctttgact tcctgaagag caccttcggt
     4021 tccagcgtcg agctgtatga cgatctgctg cccaacgccg gtgcgatctc cagctacctg
     4081 cccgctgccg agagcgacgt ctacaaccag ccctccgagt tctacggcgg ccagaccgtt
     4141 tacaaggaca tcgttgagtt tgcaggcaag gtccccgcat tcgactgcgg cgcttactac
     4201 tctgacgtgc gcagcgccct gaccgatgcc gtcaccaacg tcgtccagaa caacgccgac
     4261 attgacagcg agatgcagaa cgcccaggat accgtggagt tcaacatcgc tggctaagca
     4321 aaacatgaca catactgtgt ccggttacaa ctgagaattt gccaagggca cggtttgcat
     4381 ctgatacgtc cgctgcaagc cgcgcccttg cttttgtggc aaaataggag acactacgat
     4441 gaagaaaaaa ggactgaata tggagcagaa gcgcagcctg acgggttggg ccttcctgct
     4501 gccggcagca ctcctgatat ttgtattctg cttttacccc atggtgcagg cgctgatcct
     4561 ttcgttccag aaaggcaccg gcagcgctgt acagcctgcg ggctttgcca actacgcgcg
     4621 cattttaaag gatgccacgt tccagcagtg cctgtttaac acgattttct atttcgtgat
     4681 ccaggtgccc attatgctga tcctggcatt gatcctggcg cagctgctca acagcccgga
     4741 cattaagggc aagggcatct accgcacgat gattttcctg ccctgcgcca cgtcgcttgt
     4801 ctcctactcg atgattttca aatccctgtt tgccaacgat ggtctggtca accgggtgct
     4861 gtccaccgtg ggcatcccca cagtggattg gttccagaac gcatgggcgg cacgctgggt
     4921 catcgttatt gcgctgctct ggcgctggac cggctacaac atggtttttt atcttgccgg
     4981 tttgcagaac attgactact cgatctacga ggcggcccgc atcgacggtg cctccccgct
     5041 gcagcagttt gttcacctga cgatccccct gctgaagccc accatcctgc tgactgccat
     5101 catgtccacc tccggcacgc tgcagctgtt tgatgagtcc gtcaacctga cggcgggcgg
     5161 tcccggcaag gcaaccatga cactgaccca ctatatttat aacatctcgt ttgtcgagac
     5221 gcccaagttc aactatgcgg cggcgctgtc ggtgtttatc cttgtggtcg tggcagtgct
     5281 gtctgccatc cagatgaaag taggtgataa gcgtgactaa gggtaaaaag attctggcgc
     5341 acgctgtgct gattttggcc agcttcttgt ctgtgttccc gctgtactat atgctgtgcg
     5401 gcgcgacgaa caccagcatt gatgttgtgc gcggcaagct gatccccggc acctatctgg
     5461 tagaaaactt caagtcgctg gttgccaacc agaatctggg gctggcaatg gcaaactcct
     5521 tccgcaacgc catcctgatg acactcatca cgctgctggt ctgctccatc gcgggctatg
     5581 gctttgaaat ttaccacgat aaggcgaagg acgccctgat gagcgtcctg ctgctggcga
     5641 tgatgctgcc gttcgtggcc attatgatcc ccctgttcaa gatgatgtcc cactgggggc
     5701 ttgtcaacag ctgggcggcg tttgtgctgc cgtccatctc gacgccgttt atgatcatgc
     5761 tgttccggca ggcatcgcgt tccttcccca atgacattat tgaagcggcg cgtctcgacg
     5821 gtttgagcga gatcggcatc ttcttccgga tgtttgtccc catcatgcgc tctacctacg
     5881 gcgcggcgat gactgtcacg tttatgaacg catggaacag ctacctctgg ccgaagatcg
     5941 tattccaaag caacgcatcc atcacaatgc cgatgctggt cgcaaacctc aagagcggct
     6001 acagtgtgga ctacggtatg ctgatgctgg gcgttctgat ctgcacactg cccaccgcga
     6061 tcatcttcct ctgcctgcag aagagctttg ccaacggcat cacgggtgca gtcaagtgat
     6121 ggacgaacga acctactgcc tgacgccgga gcagtggcgt cacgccaaag cctgcctgac
     6181 gctggcaaag cgctgggggc tgatcgacga cgacagcccc gaggcgctgg aacgccgccg
     6241 ggcggccaaa aacgcgcagg cggaaagagc ggagcaggac ggcaagcccg tgtacggcac
     6301 gcggcgcttc tctgcccctg cctacctgca atatgagctg acccgcttca agctggattt
     6361 cgcggagccg tgcgaaaaaa tccgtgcgct gggtatctgc ccggatttta ccgatgcaca
     6421 gacccgcgcc tggtatgacg cgaatccgga tctgttcacg cggtacgccg gcgacagctt
     6481 cccctatgag gaagtaaagc aaatcatcga aaagcggatg cgggaggagg tgtacaacag
     6541 ccttgtgcaa gacctattac gtggacccac agacaggccg tgacaccaac gacggcctga
     6601 cgccggaaaa accactggcc acactgtttg ccgtcaaccg gctggccctg gcccccggtg
     6661 acacggtgct gctgaaatgc ggcagcgtgt ttgaaaagca gttcctgcac ctgcgctgct
     6721 gcggcgaaaa aaattgtccc atcaccatcg ctgcctatgg tgaaggcagc gccccgcgca
     6781 ttgatgctga cgggcagggg ctttggtatc aggactatgg ctgcgcactg gattcaccca
     6841 cccatgtgta ccgcgggtat gtttcctccg ccgtgctgct gtatgatgca gcctatgtca
     6901 cggtgcgtga tctggaactg acgaaccgtg ccgacgctgt catcggcgag caatattccc
     6961 agccggataa gctggagcgc accggcgtgg ctgtggtggc gaaggacagg ggcacacgct
     7021 gcggcatcac actgcaaaac ctgctgatcc atgatgtaca cggcaacgtg tacgacaagc
     7081 acatga
//
LOCUS       DBMDSQ010000077         7010 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_2816_length_7010_cov_2.072948, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000077 DBMDSQ010000000
VERSION     DBMDSQ010000077.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 7010)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7010)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7010
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2816_length_7010_cov_2.072948"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            168..1298
                     /gene="rffA"
                     /locus_tag="ACZPAX_04190"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             168..1298
                     /gene="rffA"
                     /locus_tag="ACZPAX_04190"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HIR1113129.1"
                     /translation="MIPFNVPPCVGDELEYVKQAIDSHKICGDGAFTKQCNAWLEERF
                     HAQKVLLTTSGTTALDMAMLLCDIQPGDEVILPSFTFSSTATSAVLAGAKLVFVDVRP
                     DTMNIDEKKIEQAITDKTKVIVAVHYAGVACEMDTIMDIARRHNLKVVEDAAQGVMSS
                     YKGKALGTIGDFGCYSFHETKNYSMGEGGALVINNPDYNERAEILREKGTNRAKFFRG
                     QVDKYTWVDFGDSYLPSELNAAYLWAQLLHADEINDNRMATWNAYYAAFKPLADAGKV
                     ELPTIPEGCVHNAHMFYLKCKNLEERTAFIRHLKANDILAVFHYIPLHSAPAGKKFGR
                     FDGEDVYTTAESERLVRLPMYYGLTDADREKVIAKVLEFYAQ"
     gene            1288..2778
                     /locus_tag="ACZPAX_04195"
     CDS             1288..2778
                     /locus_tag="ACZPAX_04195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113130.1"
                     /translation="MHSKRLPGWARAACTAAAALVFLLVYEWAVYKVPVTQIFLPTSS
                     WSDEVIYSKQLAAAVRYGAPQGYFGFNESHAEVGTYAAWGPAVFFVYALPGLLLRGQN
                     AFLWCNLLFVAAGWTFFALAARLSWKRQVVFALALAAMNAPIRYVFSAMQEPLHYALV
                     LAVLGCGILARRDRNRAAWGVLCALCAVATLVRPYEAVLWLYPLALAWRDRRRAAVCV
                     AGGAVSLGGTLVLMSKFYAPYFFTNVDMGALQEAAHGHLLEAAKDMARKLIGAMQTVF
                     AAMASDFANRTGGYYLLLFALLAVVIVCLAVDARRGQPIFWKGCAAVTTVIVFLALML
                     MYRPGEGSRHTLVLDVLLLASLLVEDARPAGGTALAAAALALLGVVNLADGYTMPHYS
                     AEWDAEVQQVETVLTHYRADSDAPWNNTVAYAFGDPVHCGLLYGVPDGMGIQFDEATY
                     LTDPNHEIYSRYVMTAADTPTAARLDAEGWQVIYQSEKCMIYMRTM"
     gene            2894..3433
                     /locus_tag="ACZPAX_04200"
     CDS             2894..3433
                     /locus_tag="ACZPAX_04200"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR1113131.1"
                     /translation="MNTPQLPILDGAAVMLRPITDADTDLIVKWRNTPSVVQNFIFRQ
                     TFTPEMHRSWLATKVATGQVVQYIIVDKADDKPVGSVYYRDIDPHNRSAEYGIFIGEE
                     SARGKGLGTETARLFTDFGFAGLHLHRISLRVLAENTAARRSYEKAGFVQEGIFRDME
                     LLDGQYRDVVFMARLAEKE"
     gene            3430..4947
                     /locus_tag="ACZPAX_04205"
     CDS             3430..4947
                     /locus_tag="ACZPAX_04205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113132.1"
                     /translation="MMKAKPMQKVKTFLEKTPNALLCVLGAVVFLAGFYFLTSRTPLN
                     KIWLPTTMNNDEVLYNRQVVSVITHGGPQGYFGYQEGTADIGRYGTWGPLLIWAYALP
                     GFLFGSSVNVVLWCNLLFITLGIAAFARSAKLNWWQCIVLCASLFAIAMPIRSCISGA
                     SEAMHYMLAFLIVGSAAALHRTDKTGWLAVCAAACAVETIFRPYALLFWVFPLTAVWQ
                     NKRRRNACFVTAAAGFGVSLFAMAKLAAPYFSDGGMDFDGVQLLLQGHPIAAIRYEWI
                     RATALLGNAWLYDIWPTIKGGKTYIGGGCVTFLVVVLAAVGCLLWDKRKGRPVVLKAC
                     ALFCSAAIALVLLTMYNIDPRHMMLLAILLLGAIVVEDAAPAVVWLPILVVLLLPMNF
                     ERGSQPEMNEVMAAQMTVVEDALAADVQTAGADPWDNTLAYTYDDGVFHGYLYAVPDG
                     MGIEFDKNSYLWDAENPIYSRYVMCGHGTRVEERLMAENWQELVSTEDLVVYKRS"
     gene            4964..5905
                     /locus_tag="ACZPAX_04210"
     CDS             4964..5905
                     /locus_tag="ACZPAX_04210"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR1113133.1"
                     /translation="MQKISYVIPCYRSQHTVGGVVAEIAATMQTMPQYDYEVILVNDC
                     SPDNTIGTIRSLVAADPHVLGVDLAKNFGQHAALMAGFHKCSGDIVVCLDDDGQTPAD
                     EVGKLLERIDAGYDVVYASYDSKRQAGWRNFGSWVNSKMTEIMLGKPPELVVNSYFAA
                     RRFVVDEMLRYEHCYPYVIGLVLRSTKHICNVPVHHRAREEGRSGYTLSKLLNLWMNG
                     FTSFSVKPLRIATYFGTLFAAAGFLYLIYIIVSHFTSAGAPIGWASTTALLLLLGGMI
                     LVVLGIMGEYVGRIYMCANAAPQYVEREVIRHEGDNA"
     gene            5902..6261
                     /locus_tag="ACZPAX_04215"
     CDS             5902..6261
                     /locus_tag="ACZPAX_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HIR1113134.1"
                     /translation="MKQAKWFLLLHIILGIYAGSGVCSKLAAQQPFLSAAFIVLYGLM
                     LLALVVYAVGWQQVIKHLPLTTAYANKAVTVVWGILLGLAVFGEAVTPRQVIGAVIII
                     CGIVLFVRADNEGEGGQ"
     gene            6258..6605
                     /locus_tag="ACZPAX_04220"
     CDS             6258..6605
                     /locus_tag="ACZPAX_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HIR1113135.1"
                     /translation="MNQAQLPYVLLYLCSTFLSSVSQVLLKKAALREHKSVLAEYTDW
                     RVILGYGLFVGCTLLTMLAYKGVPLNVGPVLEATGYIYVTIFGVTIFHEKMNPKKIIA
                     LAMILVGILVYAL"
     gene            complement(6763..>7010)
                     /locus_tag="ACZPAX_04225"
     CDS             complement(6763..>7010)
                     /locus_tag="ACZPAX_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HIR1113136.1"
                     /translation="FNSVTLMLAYYRSAVFDAPRRRWMRPAAMVLCMSITLSTMLLKQ
                     HSVIDVVFGLLLALALDYGATVLQSEDVGHRRLPERL"
BASE COUNT         1290 a         2108 c         2172 g         1440 t
ORIGIN      
        1 cgtcatgctt tggttcgccg ccagcatgat ccgcgcctac aacgacgtcc gcgtaaaaaa
       61 ataaggcgat tttccgcaaa tccgttccgc gataaagtga caaataccgc aaactgtgtt
      121 atactaaaca cgattataaa aattcagtcg gaaagaaggc tttactcatg atccctttta
      181 acgtgccccc ctgcgtgggc gatgaactcg aatatgtcaa acaggcgatc gactcgcaca
      241 agatctgcgg cgacggcgct ttcaccaaac agtgcaatgc ttggctggaa gagcgtttcc
      301 acgcccagaa ggtgctgctg actaccagcg gcaccactgc gttggacatg gccatgctgc
      361 tgtgcgacat ccagcccggc gacgaggtca ttctgcccag cttcaccttc tccagcaccg
      421 ccacttccgc cgtgctggcc ggggccaagc tcgtctttgt ggatgtgcgc cccgacacga
      481 tgaacattga cgagaagaaa atcgagcagg ccatcaccga caagaccaag gttatcgtgg
      541 ccgtccacta cgccggtgtt gcctgcgaga tggacaccat catggacatt gcccgccgcc
      601 ataacctcaa ggtcgtcgag gacgcagccc agggcgttat gagcagctac aagggcaagg
      661 cgctgggcac catcggtgat ttcggctgct actccttcca cgagacgaaa aactactcga
      721 tgggtgaggg cggtgcgctg gtcatcaaca atcctgacta caacgagcgc gcggagatcc
      781 tgcgcgaaaa aggcaccaac cgcgccaagt tcttccgcgg ccaggtggac aagtacacct
      841 gggtcgattt tggcgacagc tacctgccca gcgagctgaa tgcggcctac ctgtgggcgc
      901 agctgctcca cgccgacgag atcaacgaca accgcatggc gacctggaac gcctactatg
      961 cggcctttaa gccgctggcc gacgcgggca aggtcgagct gcccaccatc cccgagggct
     1021 gcgttcacaa cgcgcatatg ttctacctca agtgcaagaa tcttgaggag cgcaccgcgt
     1081 tcatccgcca tttgaaggcc aatgacatcc tggcggtgtt ccattatatc ccgctgcact
     1141 ccgccccggc gggcaagaag ttcggccgtt tcgacggcga ggacgtctac accaccgccg
     1201 agagcgagcg cctggttcgc ctgccgatgt actacggcct gaccgatgcc gaccgcgaga
     1261 aggtcattgc gaaagttttg gagttctatg cacagtaaac gcctgcccgg ctgggcgcgg
     1321 gcggcctgca cggcggctgc ggcgctggta tttctgctgg tgtacgagtg ggccgtctac
     1381 aaagtgccgg tcacgcagat tttcctgccc accagctctt ggagcgacga ggtcatttac
     1441 agcaagcagc tggccgccgc tgtgcggtac ggcgcaccgc agggatactt cggcttcaac
     1501 gagtcccacg ccgaggtggg cacctacgcg gcctggggcc cggcggtgtt cttcgtctac
     1561 gcgctgcccg ggctgctgct gcgcgggcaa aatgcgttcc tctggtgcaa cctgctgttc
     1621 gtcgcggcag gttggacgtt tttcgccttg gccgcgcggc tgagctggaa acgccaggtc
     1681 gtgtttgcgc tggcgttggc ggccatgaac gcaccgatcc ggtatgtgtt ctctgccatg
     1741 caggaaccgc tgcactatgc gctggtgctg gccgtgttgg ggtgcggcat cctggcccgg
     1801 cgggaccgga accgcgccgc ctggggcgta ctctgcgccc tgtgtgccgt ggcgaccctt
     1861 gtgcgcccct atgaggccgt gctctggctc tacccgctgg cgctggcctg gcgcgaccgc
     1921 cgccgcgccg ctgtttgcgt tgcgggcggg gctgtgtcgc tgggcggcac gctggtgttg
     1981 atgtccaaat tttatgcgcc ctatttcttc acaaacgtcg atatgggcgc tttgcaggag
     2041 gccgcccacg gccatctgtt agaagcggcc aaggatatgg cacgcaagct cattggtgca
     2101 atgcagacgg tttttgccgc aatggcgtcg gattttgcaa accgcaccgg tgggtattac
     2161 ctgttgctgt tcgcgctgct ggccgtcgta attgtctgcc tggctgtgga tgcccggcgc
     2221 gggcagccca tcttctggaa gggctgcgcc gctgtcacga cggtcatcgt ttttctggcg
     2281 ctgatgctga tgtatcgccc cggcgagggc tcgcgccaca cgctggtgct ggacgtgctg
     2341 ctgctggcaa gcctgctggt ggaagatgcc cgcccggcgg gcggcacggc gctggctgcg
     2401 gccgcgctgg cactgctggg tgtggtgaac ctggccgacg gctacacgat gccccattac
     2461 agcgccgagt gggacgccga ggtccagcag gtggaaaccg tgctgaccca ttaccgtgcc
     2521 gacagcgacg ccccctggaa caacacggtg gcctacgcct tcggcgaccc ggtacactgc
     2581 ggcctgctct acggcgtgcc cgacggcatg ggcatccagt ttgacgaggc cacctacctg
     2641 accgacccga accacgaaat ttattcccgc tatgtgatga ctgccgccga cacccccacc
     2701 gccgcccgac tggacgccga agggtggcag gtgatttatc agagtgagaa gtgcatgatt
     2761 tatatgcgca ccatgtaggg accgggcatg cccggccctt gcgtataggg cagacggccg
     2821 ttttccaccg cgttgcgggc cgcacatgtg cggcccctac aggacagcag aatgtaaaaa
     2881 taggtgagac tttatgaaca caccccaact tcccatcctg gacggcgcgg cggtgatgct
     2941 gcggcccatc acggatgccg ataccgattt gatcgtcaaa tggcgcaaca cgccgtctgt
     3001 ggtgcagaac ttcatcttcc gccagacctt cacgccggaa atgcaccgca gttggctggc
     3061 aacgaaggtt gccaccggcc aggtcgtgca gtatatcatt gtggataagg ccgacgacaa
     3121 gccggtcggc tcggtctatt accgcgatat cgacccccac aaccgcagcg ccgagtacgg
     3181 catcttcatc ggcgaggaga gtgcccgcgg caagggtctg ggcaccgaga cggcccgcct
     3241 gttcaccgat ttcggctttg cggggctgca tctgcaccgt atcagtctgc gcgtgctggc
     3301 cgaaaacacc gccgcccgcc gcagctatga aaaggccggt tttgtgcagg aaggcatctt
     3361 ccgcgatatg gaactgctgg acggccaata ccgggacgtt gtgtttatgg cccgcctggc
     3421 ggagaaggaa tgatgaaagc aaaaccgatg caaaaagtca aaacgtttct cgaaaagacc
     3481 cccaatgcgc tgctctgcgt gttgggggca gtcgtgttcc ttgcgggatt ttacttcctg
     3541 accagccgca ccccgctgaa caaaatctgg ctgcccacca cgatgaacaa cgacgaagtg
     3601 ctttataatc gccaggttgt cagcgtcatc acccacggcg gcccgcaggg gtactttggc
     3661 taccaggagg gcacggcgga cattggccgc tatggcacct gggggccgct gctcatctgg
     3721 gcctacgcgc tgccgggctt tctgttcggc agcagcgtca acgtggtgct gtggtgcaac
     3781 ctgctgttta tcacgctggg cattgcggcg tttgcccgca gcgctaagct gaactggtgg
     3841 cagtgcattg tgctctgcgc cagtttgttt gccattgcca tgcccatccg ctcctgcatc
     3901 agcggcgcat cggaggccat gcactatatg ctggcgtttc tcatcgtggg cagtgctgcg
     3961 gcgctgcacc gcaccgataa gaccggctgg ctcgccgtct gcgcggcggc ctgcgcggtg
     4021 gaaacgatct tccgccccta cgcgctgctg ttctgggtgt tcccgctgac ggcggtgtgg
     4081 cagaacaaac ggcgccgcaa cgcctgcttt gtcacggcag cggcggggtt cggcgtttcg
     4141 ctctttgcga tggctaagct ggccgcgccc tatttcagtg acggcggcat ggattttgac
     4201 ggcgtgcagt tgctgctgca gggccacccc atcgcggcga ttcgctatga gtggatccgc
     4261 gcaacggccc tgttgggcaa cgcctggctc tacgatatct ggcccaccat caagggcggc
     4321 aagacttaca tcggcggcgg ctgcgtcacc ttcctggtgg tcgtgctggc ggcggtgggc
     4381 tgcctgcttt gggataagcg caagggccgc ccggtggtgc tgaaagcctg tgccctgttc
     4441 tgcagcgccg ccattgcgct ggtactgctg acgatgtata acatcgaccc gcgccacatg
     4501 atgctgcttg ccattctgct gctgggagct attgttgtgg aggacgccgc ccctgcggtg
     4561 gtctggctgc cgattctggt cgtcctgctg ctgcccatga actttgagcg tggcagccag
     4621 ccggaaatga atgaggtcat ggccgcccag atgactgtgg tcgaggacgc cctggctgcc
     4681 gatgtgcaga ccgccggtgc cgatccctgg gacaacacgc tggcctacac ctacgacgac
     4741 ggcgtgttcc acggctacct ctacgccgtg ccggacggca tgggcattga atttgacaaa
     4801 aacagctacc tgtgggatgc cgaaaacccg atctattccc gctatgtcat gtgcggccac
     4861 ggcacccgcg tggaggaacg cctgatggcc gaaaactggc aggagcttgt ctccaccgag
     4921 gatctggtcg tttataagcg cagttgagag gatgtacaca gctatgcaga aaatttctta
     4981 tgtgattccc tgctaccgct cccagcatac ggtgggcggc gtggtggcgg aaattgccgc
     5041 cacaatgcag acgatgcccc agtatgacta cgaggtcatc ctcgtcaatg actgctcgcc
     5101 tgataatacc attggcacca tccgcagcct ggtggcggcg gacccccatg tgctgggcgt
     5161 ggatctggcg aaaaacttcg gccagcatgc tgcgttgatg gcgggcttcc acaagtgcag
     5221 cggtgatatc gtcgtctgct tggacgacga cggccagacc cctgccgacg aggtgggcaa
     5281 gctgctggaa agaattgacg cgggctacga cgtcgtctac gccagctatg acagcaaacg
     5341 ccaggcgggc tggcgcaact ttggcagctg ggtcaacagc aaaatgaccg agatcatgct
     5401 gggtaagccg ccggagctgg tggtcaacag ctactttgcc gcccgccgtt ttgtggtgga
     5461 cgagatgctg cgctatgagc attgctaccc atatgtgatc ggccttgtgc tgcgctccac
     5521 caagcatatc tgcaacgtgc cggtacacca ccgcgcccgc gaggaaggcc gcagcggcta
     5581 taccttaagc aaactgctga atctctggat gaacggcttt acgtcgtttt ccgtcaagcc
     5641 gctgcgcatt gccacctact ttggcacact gtttgcggcg gcgggctttt tgtatctgat
     5701 ctatattatc gtcagccact tcaccagcgc aggcgcaccc attggctggg cgtccaccac
     5761 ggcgctgttg ctgctgctgg gcggcatgat tctggtcgtg ctgggcatca tgggtgagta
     5821 tgtcggccgc atctatatgt gtgccaacgc cgccccccag tacgtcgagc gcgaggtcat
     5881 ccggcatgag ggggacaacg catgaagcag gcaaagtggt tcttgctgct gcatattatt
     5941 ttgggtatct atgcgggcag cggcgtgtgc agcaagctgg ccgcgcagca gccgtttttg
     6001 agcgcggcgt tcatcgtgct gtacggcctg atgctgctgg cgctggtcgt ctacgcggtg
     6061 ggctggcagc aggtcatcaa gcatctgccg ctgacgaccg cctacgccaa caaggccgtc
     6121 accgtcgtgt gggggatcct gctgggcctg gccgtctttg gcgaggccgt cacgccgcgg
     6181 caggtcatcg gcgcggtcat tattatctgc ggcatcgtgc tgtttgtgcg tgccgataac
     6241 gaaggggagg gcggccaatg aaccaggcac agctgcccta cgtgctgctg tatctgtgca
     6301 gcacgttttt atcctcggtc tcacaggtgc ttttgaaaaa ggccgccctg cgggagcaca
     6361 agtccgtcct ggctgagtac accgactggc gcgtgattct gggttacggc ctgtttgtgg
     6421 gctgcacgct gctgacgatg ctggcctaca agggcgtgcc gctgaacgtc ggcccggtgc
     6481 tggaagcgac cgggtatatc tacgtcacga tcttcggtgt gacgatcttc catgagaaga
     6541 tgaaccccaa gaaaatcatt gcgctggcga tgatccttgt gggaatattg gtgtatgcgc
     6601 tgtaaataaa aaggccccct tgttaaaggg ggctgtccgc gtgagcggac tgggggattg
     6661 ctgcgacctt gcgggagtgt gcacgcttgc cgtgccgccg caggaatccc tccacctcgc
     6721 ttacgctcgg cacctccctt taacaaggga ggcttttgtt ttttataacc tctccggcag
     6781 acgcctgtgc ccgacgtcct cgctctgcaa gaccgtggca ccatagtcca gcgccaaggc
     6841 cagcagcagg ccgaacacca cgtcaataac gctgtgctgt ttgagcagca tcgtggaaag
     6901 cgtgatggac atacacagca ccatggcagc ggggcgcatc cagcggcggc gcggggcgtc
     6961 aaaaaccgca ctgcggtagt aggccagcat cagcgtgacc gagttgaaga
//
LOCUS       DBMDSQ010000078         7004 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_2817_length_7004_cov_1.778357, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000078 DBMDSQ010000000
VERSION     DBMDSQ010000078.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 7004)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7004)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7004
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2817_length_7004_cov_1.778357"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..371)
                     /locus_tag="ACZPAX_04230"
     CDS             complement(<1..371)
                     /locus_tag="ACZPAX_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015537848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1113137.1"
                     /translation="MPRPGRMTTERAKDFKGTLRQLLSALSKYKLSLIVVVIFAVLST
                     IFNIAGPKILAKATTALATGWIAKLRGVGGIDFAYIGKVLLFLAGMYLLSAAFSFIQG
                     WLMSGLSQKVCYDFRDQISKK"
     gene            complement(373..2577)
                     /locus_tag="ACZPAX_04235"
     CDS             complement(373..2577)
                     /locus_tag="ACZPAX_04235"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005923293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1113138.1"
                     /translation="MTKIFKQLGRHWAACLAVVALLVVQAYCDLSLPDYTSKIVDTGI
                     QQGGIESPVPETVRDTTLQALELLMSEQDAVLAEQWYSAPDADGVRTLSHDATAAMSE
                     LENAFATPDVVLYMAAAQNAATAAGTTDTVTPTSYDLDAVATQFAAMAQAPGAREMLQ
                     AQLADAAPQDDSVADSLSSQAMLLVALEYDAQGIAHDVQMSYLLRTGGQMLALTLLMV
                     AVAIAVGFIASRVSAAIGRDLRRDVFKTVVGYSNAEIEKFSTASLITRTTNDIQQVQF
                     TCVILLRMVAYAPILGIGGILHVASGNTGLEWIIFVAVAALLVLITVLMNVAMPKFKQ
                     MQTLVDRLNLVSREILTGIMPIRAFSREKFEEERFDKANTDLMKTQLFTNRTMVAMMP
                     FMTLIMNGTSLLIVWFGGKAMDLGNMQVGEMIAFITYTMQIVMSFLMLSMVAVMLPRA
                     GVAADRIDEVIKTPAAIHDPAAPKALPEGGTKGVVAFNDVSFRYPGSDEDALEHISFT
                     AKPGQTTAIIGSTGCGKSTLLNLIPRFYDVSEGSVTIDGVDVRDMSQAQLHDQLGYVP
                     QKGVLFSGTIDSNLKFGGDHITDADVRRAAEIAQAAEFISAKPEGYNAPIAQGGSNVS
                     GGQKQRLSIARAIAKHPQIYLFDDSFSALDYKTDVALRRALKAQTDNATVIIVAQRIS
                     TVLHANQILVLDEGRIVGKGTHAQLMESCPEYQEIARSQLSQKELNLQKEGE"
     gene            complement(2561..3070)
                     /locus_tag="ACZPAX_04240"
     CDS             complement(2561..3070)
                     /locus_tag="ACZPAX_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005923297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIR1113139.1"
                     /translation="MSIPETPNLHGVFRFVARLHTLSKRGMRAALANEPKCRYGMLQH
                     LYNLIEQHGYDGAIYVSDIARYMHQPMPAVSRGLRAMEQDGHITRETDPGDRRKTLVR
                     ITPEGEHARSANEQAMNAYFARIMARLTPEELAQANAVKDALMAAIEAENESMQQTRK
                     GEPPHDQDI"
     gene            complement(3267..4823)
                     /locus_tag="ACZPAX_04245"
     CDS             complement(3267..4823)
                     /locus_tag="ACZPAX_04245"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012516.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HIR1113140.1"
                     /translation="MKQSKLKLRCTEDYAVLYWDKPAAAPKGAEYTVSLNGEVIGRTD
                     KTHFTIEGLPYGSTYRLEVASGSYCVGVETLQFGKEKRRVNVTAAPYFCKGDGKTLNT
                     DNLQRAINDCRADDILYFPAGDYLTGALQLHSDLEIYLSKGAVLQGTANINDYSPRIP
                     SRFEGMEMRCYSSLLNAGRLDHTKGSGCHNIIVRGKGTICGGGQVLAKKIIEDERARI
                     KSFLDDNEELVKSCENDDTIPGRVRPRLVNLSNCENVWFSGITFKNSPAWTFHMVYCD
                     NIQTDHCVFESEGVWNGDGWDPDSSTNCTLFASEFHTGDDAVAIKSGKNPEGNEIGRP
                     CAHIKVFDCRIGYGHGICIGSEMSGGVEDVQIWDCDFANSLYGIEIKATPKRGGYVRG
                     VTVRDCTAPRVILHSVSYNDDGEPAPEPPKFSHCWFEGLTLTGRPLDHDHIWQDAAPI
                     EIVGFDCHGGEIEDITFKDITITKESAELKLGLCSGVNLYNVTLSKPSPSGEGVPAGP
                     EEGRVDPGNP"
     gene            5307..>7004
                     /locus_tag="ACZPAX_04250"
     CDS             5307..>7004
                     /locus_tag="ACZPAX_04250"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012789.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HIR1113141.1"
                     /translation="MDKPEFASAHMAALNPQQRAAVEAVHGPVLLLAVPGSGKTTVLI
                     TRLGYMTLVCGIPPQSILTMTYTVAATREMRARFAVRFGDALAGQLEFRTINGVAARI
                     IALYSRMYNRTPPDLIQNENDTAPLLTRLWQETNHEYPTESTLKDLRTAITYIKNMCL
                     TDDELDALETDIDNLPELYRGYQRALKAGHRMDYDDQLCFALQILRGAPAVAKAFRER
                     YQYFCVDESQDTSKVQHEIIRVLAQESGNIFMVGDEDQSIYGFRAAYPQALMDFEKTY
                     PGAQILLMEENYRSREPILDAANRFVARNRYRRPKTIQPTQGAGEPLQIVTVRRRADQ
                     LPFLFETAQNCETETAVLFRNHESALPIIDLCERRGVPYGCKNIDPTFFSNKIVRDVA
                     DIFALAARSADADVFLRCYYKFGVPVTKVQALYACSQARQYGQGCWTALLNEDSIRPR
                     IRAAMADVAHGLARLPRMAADDAVRFVTDELGYGKYLDKKGMDRTKLAVLEMLGAQED
                     SPAHLLKRLEILRGIVQDHITPPGCRFILSTIHSAKGLEYDRVILLDEPTNHLDIESI
                     "
BASE COUNT         1385 a         2030 c         2120 g         1469 t
ORIGIN      
        1 atttttttgc tgatctggtc gcggaaatca tagcagacct tctgggacag accgctcatg
       61 agccagccct ggataaagct gaatgccgcg gaaagcaggt acattcccgc caaaaacagc
      121 agcactttcc caatataggc aaagtctatg ccgcccacgc cgcgcagctt ggcaatccat
      181 ccggtggcca gcgccgtggt ggccttggca aggattttcg gccctgcaat gttgaaaatt
      241 gttgacagaa cggcgaagat aacgaccacg atgagcgaca gcttatactt gctcaacgcg
      301 gaaagcagct gccgcagcgt gcccttaaaa tccttggcgc gctcggtcgt catgcgtccg
      361 ggtcttggca tatcactcac cctccttctg caaattgagt tccttctggc ttaactggct
      421 gcgggcgatc tcctggtact ctgggcagga ttccatcagc tgggcgtgtg tgcccttgcc
      481 gacgatgcgg ccctcatcca gaaccaaaat ctggttcgcg tgcagcacag tggaaatgcg
      541 ctgcgccacg atgatgacgg tagcgttgtc ggtctgggct ttcagtgcgc gacgcagcgc
      601 cacgtcggtt ttgtagtcca gcgccgagaa actgtcgtca aacaggtaaa tctgcgggtg
      661 cttggcaatc gcgcgggcga tggacaaccg ctgtttctgc ccgccggaga cattgctgcc
      721 gccttgggca atcggagcgt tgtagccctc gggcttggcc gaaataaatt cggcggcctg
      781 ggcaatctca gcagccctgc gcacgtcggc gtcggtgatg tggtcgccgc caaatttcag
      841 gttggaatca atggtgccgc tgaacagcac acctttctgc ggcacgtagc ccagttggtc
      901 gtgcagctgc gcctggctca tatcgcgcac atcgacgccg tcgatcgtca cactgccttc
      961 gctgacgtcg taaaaacgcg ggatgaggtt gagcagcgtc gatttgccgc agcctgtgga
     1021 gccgatgatg gccgtcgtct gcccgggttt ggcggtaaag ctgatatgct ccagcgcgtc
     1081 ctcgtcagag ccgggatagc ggaagctgac atcgttaaaa gccacaacgc ccttggtgcc
     1141 gccctcgggc agggctttcg gcgcggcagg gtcgtggatg gcagcggggg tcttgatgac
     1201 ctcgtcgatg cggtctgcgg ccacacccgc gcggggcagc atgacggcga ccatcgagag
     1261 catcaggaac gacataacga tctgcatggt gtaggtgatg aaagcaatca tctctcccac
     1321 ctgcatattg cccaggtcca tggccttgcc gccaaaccag acgatgagca ggctcgtgcc
     1381 gttcatgata agggtcataa acggcatcat ggcgaccatt gtgcggttgg tgaagagctg
     1441 cgttttcatc aggtccgtgt tggccttgtc gaagcgctcc tcctcaaatt tctcgcggct
     1501 gaaagcacgg atgggcatga tgccggtcag aatctcgcgg ctgacaaggt tcagccggtc
     1561 cacaagggtc tgcatctgct tgaatttcgg catggctacg ttcatcaaca ccgtgataag
     1621 cacgagcagc gcggccaccg ccacaaagat gatccattca aggccggtgt tgccgctggc
     1681 aacgtgcagg atgccgccga tgcccaaaat gggcgcgtag gctaccatac gcagcaaaat
     1741 cacacaggta aactgcacct gctggatatc gttggtggtg cgggtaatca gcgatgcggt
     1801 ggagaatttc tcgatttcgg cgttggagta gccgacgacg gtcttgaaca catcccggcg
     1861 caggtcgcgg ccaatggcgg cggaaacgcg ggacgcgatg aaaccgacgg cgattgctac
     1921 ggccaccatc agcagcgtca gggccagcat ctggccgccg gtgcgcagca gataagacat
     1981 ctgtacatcg tgggcaatgc cctgggcgtc gtattccagt gctaccagca gcatagcctg
     2041 gctggataag ctgtctgcca cggaatcgtc ctgcggcgcg gcgtcggcca gctgggcttg
     2101 cagcatttcc cgcgcaccgg gggcctgggc catggcggca aactgggtcg cgacggcgtc
     2161 caggtcatag ctggttggcg tcacggtgtc ggtcgtgccg gcggcggtcg cagcgttctg
     2221 ggcagcggcc atatacagca cgacgtcggg ggtggcaaac gcgttttcca gttccgacat
     2281 ggcagcggtg gcatcatgag agagggtacg cacaccgtcg gcatcggggg cactgtacca
     2341 ctgttcggcc agcacggcgt cctgctcgga cataagcaac tccagtgctt gcagggtcgt
     2401 gtcgcgtaca gtctcgggta cagggctttc aatgccgccc tgctggatgc cggtgtccac
     2461 gattttgctg gtgtagtcgg gcaggctcag gtcgcagtag gcctgcacga cgagcagcgc
     2521 cacgacggcg agacacgccg cccagtgccg ccccagctgt ttaaatatct tggtcatgcg
     2581 ggggttctcc tttccgggtt tgctgcatcg attcgttttc ggcctcgatg gcggccatca
     2641 gtgcatcctt tacggcgttg gcctgggcca gttcctcggg ggtcagccgt gccatgatgc
     2701 gggcaaaata ggcgttcatg gcctgctcgt ttgcgctgcg ggcgtgctcc ccttccggag
     2761 tgatgcgcac cagcgttttt cggcggtcgc cagggtctgt ctcgcgggta atgtggccgt
     2821 cctgctccat ggcacgcagc ccgcgggaga cggcgggcat cggctggtgc atataccgcg
     2881 caatgtccga aacataaatt gcgccgtcat agccgtgctg ctcgatgagg ttgtacagat
     2941 gttggagcat tccgtaccgg cattttggct cattggccag ggccgcacgc ataccgcgct
     3001 tggacagggt atgcagccgc gcgacgaacc ggaacacacc gtgcagattg ggtgtttctg
     3061 ggatagacat tacacgcctc ctgttaataa ttatcatgtg ttaataattg tgctgtgata
     3121 atactacacc cccttgcatc gctatgcaag ggggtgtagg gaaggtttaa gatttgttta
     3181 caaatggcag gtgaaaaggc tttccccttg ggggaagctg tccgcgaagc ggactgatga
     3241 ggagagaagt tcccgctatg acccgtttat gggttgccag ggtcaacacg cccctcctcc
     3301 ggccctgcgg ggacaccttc ccccgagggg gaaggcttgg agagtgtgac gttgtagaga
     3361 tttaccccgc tgcacagccc cagcttcagt tctgcgctct ccttcgtgat tgtgatatcc
     3421 ttaaacgtaa tatcctcgat ctccccgcca tggcagtcga acccgacaat ctcgatgggc
     3481 gcggcgtcct gccagatgtg gtcgtgatcc agcgggcggc cggtcagggt cagcccctca
     3541 aaccagcaat ggctgaactt cgggggctcg ggggcgggtt caccgtcgtc attgtaggat
     3601 acgctgtgca aaattacccg cggggcggtg caatcgcgca cggtcacgcc gcgcacatag
     3661 ccgccgcgct tgggtgtggc cttgatctca atgccgtaca gcgagttggc aaagtcgcag
     3721 tcccaaatct gcacgtcctc cacaccgccg ctcatctcgc tgccgatgca gatgccgtgg
     3781 ccgtacccga tgcggcagtc aaacaccttg atgtgggcgc agggacggcc gatttcgttt
     3841 ccctcggggt ttttgccgct cttgatggca actgcgtcgt cgccggtatg gaactcgctg
     3901 gcgaacagcg tgcagtttgt cgaggaatcc gggtcccagc cgtcgccgtt ccagacgccc
     3961 tcggattcaa aaacgcagtg gtcggtctgg atattatcgc agtagaccat gtggaaagtc
     4021 cacgccggac tgtttttgaa cgtaataccg ctgaaccaga cattctcgca gttgctcagg
     4081 tttactaagc gcgggcgcac acggccgggg atcgtgtcgt cgttctcgca gcttttgaca
     4141 agttcctcgt tatcgtccaa aaagctcttg atgcgggcgc gctcgtcctc gataattttc
     4201 tttgccagca cctgcccgcc gccgcagatg gtgcccttgc cgcgcacgat gatgttatgg
     4261 cagcccgagc ctttcgtgtg gtcaagccgc ccggcgttga gcaggctgga atagcagcgc
     4321 atctccatcc cttcaaagcg gctggggatg cggggcgaat agtcgttgat gttggccgtg
     4381 ccctgcaaaa cagccccctt ggagaggtaa atttccaaat cgctgtgcag ctgcagcgcc
     4441 ccggtcaggt agtcacccgc cggaaaatac aggatgtcat cggcgcggca gtcgttgatg
     4501 gcccgctgca agttgtcggt gttcagcgtt ttgccgtcgc ccttgcagaa atagggcgca
     4561 gccgttacgt tgacgcggcg cttttctttg ccgaattgca gcgtctcgac cccgacgcaa
     4621 tagctgccgc tggcaacttc cagccggtaa gtgctgccgt aaggcaggcc ctcgatggtg
     4681 aaatgtgttt tatccgtgcg gccgatgacc tcgccgttca gcgagacggt atactccgcc
     4741 cctttgggcg cagcggcggg tttatcccaa tagagcaccg cgtaatcttc ggtgcagcgc
     4801 agttttaatt tactttgctt catgatgttt tccttttaca cgcaagttgt ctgttgctta
     4861 catattgtac cacagcccgc cggtttatcc aatacatagg ggtgccaaaa tacagtggtc
     4921 gaaacgcggc aggttttgac agttttacag gcttgacagc gcgggaggcg acgtttataa
     4981 tcatataagc atagtgggta tatgtgctat gccgttgcag gggccgggca gatccggccc
     5041 tcaactttgc cgaacccgca gctttacggt tgagagagga tacaacgccg gtgaggtcat
     5101 caacgatatt gcgctgaacc gcgcgtgatt ggaacatcct tctcccctgc cgtttggcgg
     5161 gggagttttg cttttactgg ggaaatatga tataatttca acatccgtgc aggggccgga
     5221 cgcctccgca gggcgtcgag gacgccgccc cctacaatac acgccgccat gcgaaaaata
     5281 tttactattt gccgaaagga gccgccatgg acaaacctga atttgcatcc gcccatatgg
     5341 cggcgctgaa cccacagcag cgggcggcgg tggaggccgt acacggcccg gtgctgctgc
     5401 tggccgtgcc gggcagcggc aagaccacgg tgctgatcac ccggctggga tacatgacgc
     5461 tggtctgcgg catcccaccg cagtcgattt tgacaatgac ctacaccgtg gccgccacgc
     5521 gggaaatgcg tgcccgcttc gccgtgcggt ttggggatgc gctggccggg cagctggaat
     5581 tccgcacgat aaacggcgtg gcagcgcgca ttatcgcgct gtacagccgt atgtacaacc
     5641 gaacgccgcc cgacctgatc caaaacgaga acgacaccgc accgctgctt acacgccttt
     5701 ggcaggagac gaaccacgaa taccccaccg agagcacgct gaaggatctg cgcacagcca
     5761 tcacctacat taaaaatatg tgcctgaccg acgacgagct ggacgcgctg gaaaccgaca
     5821 tcgacaactt acccgagtta taccgcggct accagcgggc gttgaaagcc gggcacagga
     5881 tggactacga cgaccagcta tgctttgcgc tgcaaatcct gcgcggtgcg cccgctgtgg
     5941 cgaaggcgtt ccgggagcgg tatcaatatt tctgcgtgga tgaatcccag gacacctcca
     6001 aggtgcagca tgagattatc cgcgtgctgg cgcaggaaag cggaaatatc tttatggttg
     6061 gcgatgagga ccagagtatt tacggtttcc gcgccgccta cccccaggcg ctgatggact
     6121 ttgaaaagac ttaccccggc gcacaaatct tgctgatgga ggaaaactac cgctcacggg
     6181 aaccgattct ggacgcggcc aaccgctttg tggcccgcaa tcgttatcgc cgccccaaaa
     6241 cgatacagcc cacgcagggc gcaggcgaac cgctgcagat cgtcacggtg cgccgccgtg
     6301 ccgatcagct gccgtttttg tttgagacgg cgcagaactg cgagacggaa accgccgtgc
     6361 tattccgcaa ccacgagagc gccctgccca tcatcgacct gtgcgagcgc cggggcgtgc
     6421 cctatggctg caaaaacatc gaccccacgt ttttctccaa caaaatcgtg cgggatgtgg
     6481 cggatatctt tgcgctggcc gcccgttccg ccgacgcaga tgtttttctg cgctgctact
     6541 acaaatttgg tgtgccggtc acaaaggtgc aggcgctgta cgcctgcagc caggcgcggc
     6601 agtacggcca aggctgctgg acagccctgc tgaacgagga cagcatccgc ccgcgcatcc
     6661 gcgccgccat ggccgatgtg gcccacggcc tggctcgcct gcccaggatg gcggcggacg
     6721 acgccgtgcg gtttgtgacc gacgagctgg gctacggcaa atacctggac aaaaagggca
     6781 tggaccgcac gaaattggcc gtgctggaaa tgctgggtgc ccaggaggac agccctgccc
     6841 acctgttaaa gcggctggaa attctgcgcg gcatcgtgca ggatcatatt acgccgcccg
     6901 gatgcaggtt tattttatcc acgatacact ccgccaaggg gctggaatac gaccgggtga
     6961 ttttgttgga cgagcctacc aatcatctgg atatagaaag catt
//
LOCUS       DBMDSQ010000079         6995 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_2822_length_6995_cov_1.475776, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000079 DBMDSQ010000000
VERSION     DBMDSQ010000079.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 6995)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6995)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6995
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2822_length_6995_cov_1.475776"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            22..615
                     /locus_tag="ACZPAX_04255"
     CDS             22..615
                     /locus_tag="ACZPAX_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005944560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3793 family protein"
                     /protein_id="HIR1113142.1"
                     /translation="MAERTRVTPSPRSFACDLATLCAPTLAGLKPASLFRYQPAPGQD
                     AADMAAAWHAALAPRGVTVRVLKQCPRTGAVLVYVYRPARVARLLADPRTLDFLAGEG
                     YALGTADALLNQLADRLCCEGEFPHEIGVFLGYPLADVIGFIENRGKNFTACGYWKVY
                     TDPTAAQAEFDRYKKCERIYARCYYNGTPIRRLTVAA"
     gene            654..1082
                     /locus_tag="ACZPAX_04260"
     CDS             654..1082
                     /locus_tag="ACZPAX_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006598651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HIR1113143.1"
                     /translation="MSKIAVIYWSGTGNTEAMADLVANAATAAGAAVDKFTASEFNVA
                     SAGDYTGFALGCPAMGAEQLEENEFEPMYQDLRGSLSGKPVGLFGSYGWGDGEWIRTW
                     AEDAEAAGARMVVEPVMANGAPDGDAETACAALGTALANA"
     gene            1446..1820
                     /locus_tag="ACZPAX_04265"
     CDS             1446..1820
                     /locus_tag="ACZPAX_04265"
                     /inference="COORDINATES: protein motif:HMM:NF012621.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HIR1113144.1"
                     /translation="MANVIIVLILVVAVVYGIYNYVHHLNHGGGCCGEHDAPARKVKA
                     TDTNKSHYLHRLVMGVDGMTCQNCQRHVENALNAMTDTLAEADLSAQKVTVWTKADAD
                     EDAIRQAIRDAGYLPLRTTEKE"
     gene            1967..3688
                     /locus_tag="ACZPAX_04270"
     CDS             1967..3688
                     /locus_tag="ACZPAX_04270"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008120215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0042883 - cysteine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HIR1113145.1"
                     /translation="MINKRLIAMMGDSKRYIARNVALQWVALLANIAMIFAVSAFLAG
                     LVYGQGSLSAALLVLLGALVVRFFCTRGAASTSYNASRTVKKTLRAAIYEKLLRLGGS
                     YTQAVPTAEVLQLAGEGVEQLETYFGAYLPQFFYAMLAPLTLFIALAPVSLRAAVILM
                     ICVPLIPVSIVAVQKFAKKLLGKYWGQYAALGDSFLENLQGLTTLKIYQADEARHAAM
                     NREAEHFRKVTMKVLTMQLNSIIVMDVIAYGGAALGIAVAAKEFAAGRVGLQGALCIL
                     LLSADFFLPMRALGSYFHVAMNGMAASDKIFKLLDLPEGDARTAEIGTGCAIACRDLH
                     FGYTAEKETLHGLNLDFPQGSFTALVGESGCGKSTIAAVLTGRAAGYTGGVTIGGQEL
                     STVNEANLLKNVTLVSLGSHLFKGTVADNLRMAAPDADDGALWGALEQVNLADFLRSE
                     DGLNTQLAEGGSNLSGGQRQRLALARALLHDSPIYIFDEATSNIDVESENDIMAGIHR
                     LAGRKTVILISHRLANVTQADNIYVLDHGHLAGQGTHEALLAQGGLYADLWNRQQTLE
                     NYGKGAQ"
     gene            3688..5355
                     /locus_tag="ACZPAX_04275"
     CDS             3688..5355
                     /locus_tag="ACZPAX_04275"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding/permease
                     protein"
                     /protein_id="HIR1113146.1"
                     /translation="MENERTRGNFNVMCRLVGLVKPLAPVMVCAVLLGVAGFLCAIFI
                     PVLGVYALLDTLLAAAPIALGTVCILLPILAVARGVLHYAEQGCNHFIAFKLLALIRD
                     KVFGVLRQLAPAKLEGRDKGDLIAVLTADIELLEVFYAHTISPICIAVIVSIVMACFM
                     GSYAPLLGLYAALAYAMIGIGVPVATAKSARKSGENFRAEFGALNGFVLDSLRGVRES
                     LQYNDGAHRLAQINQRTDALAGQERRLKEAGGTAQAVTGAIILLLDGGMLALTGSLFA
                     SGTIGFDGALACTVALMSSFGPVVALANLGTGLQSTFAAGNRVLDILDEQPAVKEVVG
                     GKNIVFSGADTRRVTFGYGDSEILKEVTLHIPQNQVVGVTGRSGSGKSTLLKLLMRFW
                     DTDLGKVEISGENIKGINTASLRAAESFVEQDTVLFHDSIEENVKIANRDATHEQVVE
                     ACKKASIHDFIMTLPQGYDTLVGELGDTLSGGERQRLGVARAFLHDAPFILLDEPTSN
                     LDSLNEAVILHSLAEERANKTVVLVSHRASTMRIADTTYSVENGRMS"
     gene            5355..5720
                     /locus_tag="ACZPAX_04280"
     CDS             5355..5720
                     /locus_tag="ACZPAX_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005940299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HIR1113147.1"
                     /translation="MADKNVVGGGVPDTPEKKRRQEKETVGLMIELYCRGNHGTPKGQ
                     LCPDCAALRDYADARVDHCPHMATKTFCSVCKTHCYKPEMREQIRQVMRWSGPRMLFH
                     HPVLAVRHLVETRKQKKAQ"
     gene            6121..6333
                     /locus_tag="ACZPAX_04285"
     CDS             6121..6333
                     /locus_tag="ACZPAX_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005944556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HIR1113148.1"
                     /translation="MPLTMSKAGDTVTIQKITGKDEVRQHLAELGFVVGETVTVVNEI
                     SGNLIIQVKEARIALDKTLAMRIIVG"
     gene            6396..6617
                     /locus_tag="ACZPAX_04290"
     CDS             6396..6617
                     /locus_tag="ACZPAX_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HIR1113149.1"
                     /translation="MKTLKDAQVGETVTVAKLHGEGPVKRRIMDMGLTKGTKVYVRKV
                     APLGDPMELTVRNYELSVRKADAEMIEVE"
     gene            6640..>6995
                     /locus_tag="ACZPAX_04295"
     CDS             6640..>6995
                     /locus_tag="ACZPAX_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005927395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB small GTPase domain-containing protein"
                     /protein_id="HIR1113150.1"
                     /translation="MAIKIALAGNPNCGKTTLFNSLTGSNQYVGNWPGVTVEKKEGKL
                     KGHDDVVIQDLPGIYSLSPYTLEEVVARGYLVNEKPDAILNIIDGTNIERNLYLTTQL
                     IELGIPVVMAVNMIDLV"
BASE COUNT         1446 a         2092 c         2099 g         1358 t
ORIGIN      
        1 cagaggataa ggagccgtct catggcagaa cgtacccgcg ttacccccag cccgcgcagc
       61 ttcgcctgcg atttggcaac cctgtgtgcc cccacgctgg ccgggctcaa acccgccagc
      121 ctcttccgct accagcccgc gcccgggcag gacgccgccg acatggccgc cgcctggcac
      181 gcagcgctcg cgccccgcgg cgtcacggtg cgggtgctca agcagtgtcc gcgtaccggg
      241 gccgtgctgg tctatgttta ccgccccgcg cgggtggcaa gactgctggc agacccccgc
      301 acgctggact ttctggcagg ggaggggtac gccctcggca ctgcggacgc cctgctgaat
      361 cagctggccg accggctgtg ctgcgagggg gaattcccgc acgagatcgg cgttttctta
      421 ggctacccgc tggcggatgt catcggtttt atcgagaatc gcggcaaaaa ctttacggcc
      481 tgcggctact ggaaggtcta caccgacccc accgccgccc aggcagagtt tgaccgctac
      541 aaaaaatgcg agcgcatcta cgcccgctgc tattacaacg gcaccccgat ccggcggctg
      601 actgtggccg cctgacgatg caccataaag aatgtacaaa ggagtagttt actatgagca
      661 agatcgcagt tatctattgg agcggcaccg gcaacaccga ggctatggct gatttggtcg
      721 ctaatgcagc aaccgctgcc ggcgcagctg ttgacaagtt caccgcatct gagttcaatg
      781 ttgcctccgc cggcgactac accggctttg cgctgggctg ccccgccatg ggcgctgagc
      841 agctggaaga aaacgagttt gagccgatgt atcaggatct gcgcggctcc ctgagcggca
      901 agcctgtcgg cctgttcggc tcctacggct ggggcgacgg cgagtggatc cgcacctggg
      961 ccgaggatgc cgaggctgcc ggtgcccgca tggtcgttga gcctgtcatg gccaacggtg
     1021 cccccgacgg cgacgctgag actgcctgcg cagccctggg caccgccctt gccaacgcgt
     1081 gatattctcc atatccatca tacctggggg gggcagcaat gcccctcttc ttttttatgt
     1141 aaagccttct cacctgggag aaggtggccg aaggccggat gaggggcaca tttgccatag
     1201 caacccgttc ataggcaata acgccgccca tgtaggggcc gggcatgccc ggccctcagc
     1261 ttacccgcaa tatcttcctt caaagagagg tctggcaagg ccgcccctca tcagcccgct
     1321 tcgcgggcag ctttcccaaa gggggaagcc tttgcttgca gcatccaaaa agttcctact
     1381 tgacaatctc gcaaagttag tatatcctaa gttcgattag aaaacactaa cccggaggtt
     1441 atcgtatggc aaacgttatt attgttttga tcctggtcgt agctgttgtc tacggcatct
     1501 acaactatgt acatcacctg aaccatggcg gcggctgctg cggtgagcat gacgcccccg
     1561 ccaggaaggt caaggccacc gacaccaaca aaagccatta cctgcaccgt ctggtcatgg
     1621 gtgtggacgg catgacctgc cagaactgcc agcgccatgt ggagaacgcc ctgaacgcca
     1681 tgacggacac cctggccgag gcagacctga gcgcccagaa ggtgacggtc tggaccaagg
     1741 ccgacgccga cgaagacgcc atccgtcagg ccatccgcga tgccggttat ctgcccctgc
     1801 gcacgacgga gaaagagtaa acacattggg tatgtcctgt ccgtaggggc cgggcctgcc
     1861 cggttctcaa ccttgcggtg gcccacagcg tttcgggttg cggcagatgc gcgggccgga
     1921 tatgtctgtc cccctacaac tctactaaaa ggaaccacaa actatgatga taaacaaacg
     1981 cctgatcgcc atgatgggcg attccaaacg gtatatcgcg cggaatgtgg cgctgcaatg
     2041 ggtggcgctg ttggccaata ttgccatgat ctttgccgtg tctgcgtttt tggcggggct
     2101 tgtgtacggc cagggcagtc tgtccgccgc gctgcttgtg ctgctgggtg cgctcgtcgt
     2161 gcggttcttc tgcacccggg gcgcggcttc gaccagctac aacgccagcc gcaccgtgaa
     2221 aaagacgctg cgcgccgcca tctatgaaaa actgctgcgc ctgggcggca gctacaccca
     2281 ggccgtgccc accgccgagg tcctgcagct ggcgggcgag ggcgtcgaac agctcgagac
     2341 ctacttcggc gcgtaccttc cgcagttttt ctacgccatg ctcgcgcccc tcacactgtt
     2401 tatcgcgctg gccccggtca gcctgcgggc agcggtcatt ttgatgatct gcgtgccgct
     2461 gattccggtt tccatcgtgg ctgtgcagaa atttgccaaa aaactgctgg gcaaatactg
     2521 gggccagtac gccgcgctgg gtgacagctt tctggaaaac cttcaagggt tgaccacact
     2581 caaaatctac caggcagacg aagcccgcca tgcggccatg aaccgcgagg ccgagcattt
     2641 ccgcaaggtg acgatgaaag tcctgacgat gcagctgaac tccatcatcg ttatggacgt
     2701 catcgcctac ggcggcgcgg cgctgggcat tgccgtggca gctaaagagt ttgccgcggg
     2761 ccgcgtgggt ttgcagggcg cactctgcat tttgctgttg tcggcggatt tcttcctgcc
     2821 catgcgcgcg ctggggtcgt atttccacgt cgccatgaat ggcatggccg ccagcgataa
     2881 aatttttaag ctgctggatc tgcccgaggg cgatgccagg accgccgaga tcggcacggg
     2941 ctgcgccatt gcctgccgtg acctgcactt tggctacacc gccgaaaaag agacgctgca
     3001 cggcttgaat ctcgactttc cccagggcag ttttacggcg ctggtgggcg agagcggctg
     3061 cggcaaatcg acgattgctg ctgtactgac cggccgcgca gctggctaca ccggcggcgt
     3121 aaccatcggc gggcaggaac tttccaccgt caacgaagca aaccttttga aaaacgtgac
     3181 cctcgtcagt ctgggcagcc acctgttcaa gggtacggtg gcagacaacc tgcggatggc
     3241 cgcacccgac gccgacgacg gcgcactgtg gggcgcgttg gaacaggtca acctcgcaga
     3301 tttcctgcgc agcgaggatg gcctgaacac ccagctggcc gaaggcggca gcaacctgtc
     3361 cggcggccag cggcagcgtc tggcgctggc ccgcgccctg ctgcatgaca gccccatcta
     3421 tatctttgac gaagcaacgt ctaatatcga cgtagagagc gagaacgata tcatggcggg
     3481 catccatcgt ctggccgggc gcaagacggt cattttgatt tcccatcgcc tggccaacgt
     3541 cacgcaagcg gacaatattt acgtgctgga tcatgggcac ttggccggac agggcacaca
     3601 cgaagcactg ctggcccagg gcgggctgta cgccgacctg tggaaccgcc agcagacact
     3661 ggaaaactac ggaaaggggg cgcagtgatg gaaaacgaac gcacgcgcgg caatttcaac
     3721 gtcatgtgcc gccttgtggg gctggtcaag ccgctggccc cggtcatggt atgcgccgtt
     3781 ctgcttggtg tggcgggctt tctttgcgct atctttatcc cggttctggg cgtctatgcg
     3841 ctgctggaca cgctgttggc cgccgcgcct attgcccttg gcaccgtatg tattttgctg
     3901 ccgattttgg ccgtagcccg cggcgtgctg cactacgccg agcagggctg caaccatttt
     3961 attgccttca agctgctggc cctcatccga gacaaggtct ttggcgtgct gcgccagctg
     4021 gcccccgcaa agctggaagg ccgcgacaag ggcgacctga ttgccgtgct gaccgccgac
     4081 attgaactgc tggaagtttt ctacgcccac acgatctcgc ccatctgcat tgcggtcatc
     4141 gtaagcatcg ttatggcgtg ctttatggga agttatgcgc cgctgctggg gctgtacgcc
     4201 gccctggcct acgccatgat cggcatcggt gtcccggtgg ccaccgccaa gagtgcgcgc
     4261 aagtctggcg agaatttccg cgcggagttc ggcgctctga acggttttgt gctggacagc
     4321 ttgcgcggcg tgcgggaaag cctgcaatac aacgacggtg cgcaccgctt agcgcagatc
     4381 aaccagcgca ccgacgcgct ggccgggcag gagcgccgcc tgaaagaggc tggcggcacg
     4441 gcacaggccg taaccggggc catcatcctg ctgttggacg gcggtatgct ggcgctgacg
     4501 ggatccttgt tcgcgtcggg gaccatcggt tttgacggtg cactggcctg caccgtggca
     4561 ctcatgagca gctttggccc ggtcgtcgcg ctggcaaacc tcggtaccgg gctgcaaagc
     4621 acctttgccg caggcaaccg cgtgttggac atcctcgatg agcagcccgc cgtgaaagaa
     4681 gtcgtcggcg gcaagaacat tgtgttcagt ggtgccgaca cccgccgcgt gaccttcggc
     4741 tacggcgaca gcgaaatctt aaaggaagtc acgctgcaca tcccgcagaa ccaggtcgtg
     4801 ggcgtcaccg gccgcagcgg cagcggcaaa tccacgctgc tcaaactgct catgcggttc
     4861 tgggataccg atttgggcaa ggtggagatt tccggcgaga acatcaaggg gatcaacaca
     4921 gcgtcgctgc gcgcagcgga gagctttgtc gagcaggata ccgtgctgtt ccatgacagt
     4981 attgaggaaa atgtcaaaat tgctaaccgg gacgcgaccc atgagcaggt cgtggaggcc
     5041 tgcaaaaagg cgtccatcca cgactttatc atgacgctgc cccagggcta cgacactctc
     5101 gtcggtgagc tgggcgatac cctctcgggc ggtgagcggc agcgccttgg cgtggcacgg
     5161 gcatttttgc atgacgcacc gttcattctg ctggacgaac cgacaagcaa tctcgacagc
     5221 ctgaacgagg cggtcatcct gcacagcctg gccgaggaac gcgcaaacaa gaccgtcgtg
     5281 ctggtatccc accgggcatc gacgatgcgc attgccgaca cgacctattc ggtcgagaat
     5341 gggagaatga gctgatggcc gacaagaacg ttgtaggggg cggtgttccc gacaccccgg
     5401 agaaaaaacg ccggcaggag aaagaaacgg tcggcctgat gatcgagctg tactgccgcg
     5461 gcaaccacgg cacgccgaaa ggccagctct gccccgactg cgcggccctg cgggactacg
     5521 ccgacgcgcg ggtggaccac tgcccgcaca tggcgaccaa gacgttttgc agcgtctgca
     5581 agacccattg ctacaagccc gaaatgcggg agcagatccg ccaggtcatg cgttggagcg
     5641 gcccgcggat gctgttccac catcccgtgc tggcggtacg gcatctggtg gagacaagga
     5701 aacagaagaa ggcacaataa atacgcacag ggcaggggag taccttgccc tgttttgtat
     5761 tgtagggggg cgccgtcaag cggtcaagac ccttcccttg caaatccgtt ggataatggg
     5821 actgccgccg taaacgctgc gggtcgggca tgcccggccc ctacgactct ataaatgatg
     5881 tacacggcaa cacccgccgc caagcattta cgatttattc aaattttgca aaggttagtt
     5941 actgataact gtgcaatctt ccaaaagtgt tcgccgtgcc taactcacat cttgacaccg
     6001 ggagttagtg gtggtaaact accgacataa gcggttagaa cgttctaacc gcgtgctttc
     6061 atccaatatg ttagcatagg ctaatgtgtt gcagacttgc aaaaaagggg agtgtatgga
     6121 atgcctttga cgatgtctaa agcaggcgat accgttacca tccagaaaat caccggcaag
     6181 gacgaagtcc gccagcatct ggccgagctg ggttttgttg tgggcgagac cgttaccgtt
     6241 gtcaacgaga tcagtggcaa cctcatcatc caggtcaagg aagcccgcat tgcgctggat
     6301 aagacgctgg ccatgcgtat tattgtcggc tgataaaaag ggaatctcag ccataggctg
     6361 tttgatttat tgtaccttaa gaggaggaac aaagtatgaa gactctgaaa gatgcacagg
     6421 tcggcgagac cgtcaccgtt gccaagctgc atggcgaagg ccctgtcaag cgccgcatca
     6481 tggacatggg cctgaccaag ggcaccaagg tctacgtccg caaggtagcc cccctgggcg
     6541 acccgatgga actgaccgtc cgtaactacg agctcagcgt ccgcaaggct gatgctgaaa
     6601 tgatcgaggt tgagtaaatt tcagaaaggg gtataatcaa tggcaattaa aattgctctg
     6661 gcaggcaacc cgaactgcgg caaaaccacc ctgttcaaca gcctgaccgg ttctaaccag
     6721 tatgtcggca actggcccgg cgttaccgtt gagaagaagg aaggcaagct gaagggccac
     6781 gacgacgtcg tgatccagga cctgcccggt atctactcgc tgtctcccta cacgctggag
     6841 gaggtcgtcg cccgcggcta cctcgtcaac gagaagcccg atgccatcct caacatcatc
     6901 gatggcacca acatcgagcg caacctctat ctgaccaccc agctgatcga gctgggcatc
     6961 cccgttgtca tggcagtcaa catgatcgac ctggt
//
LOCUS       DBMDSQ010000080         6948 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_2840_length_6948_cov_1.647145, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000080 DBMDSQ010000000
VERSION     DBMDSQ010000080.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 6948)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6948)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6948
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2840_length_6948_cov_1.647145"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(302..1594)
                     /locus_tag="ACZPAX_04300"
     CDS             complement(302..1594)
                     /locus_tag="ACZPAX_04300"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015537305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HIR1113151.1"
                     /translation="MLTSITGINWGDEGKGRMVDLLSQDYDIVARYQGGDNAGHTVKN
                     ERGKFVLNLIPSGILRPDVVCVMGGGMVIDPEHLEKEIASLTEKGVEITPKNLKISDR
                     ATITMPFHVAQDGLEEERLSKTGAQFGSTKRGIAYSYGDKYMKKTLRMGDLVHMDASV
                     KKRLETIVESKNLTMVNIYGQKPVSVEEMWAWCEKYGKLFAPYICDVGDYLDQADREG
                     KKIMFEAQLGALRDIDFGIYPYTSSSNTVSAYAPIGAGIPGHKLNLSIGIMKAYSSCV
                     GEGPFTTELAMTEAENDALREHGHEYGAATGRPRRVGPFDAVASRYGVQCQGCDELAL
                     TLLDVLDYMEEVPIVTAYKLTDGTTTTRFPMGKTLDDAQPVVEKVPGWHCDITGVRKF
                     EDLPQATQNYVKRLEELVGCKIKYISVGPERGACIVRE"
     gene            complement(1838..2092)
                     /locus_tag="ACZPAX_04305"
     CDS             complement(1838..2092)
                     /locus_tag="ACZPAX_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019161641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 domain-containing protein"
                     /protein_id="HIR1113152.1"
                     /translation="MEKSKIDRINALARKAKSIGLTERETAERDALRKEYLTDMRASF
                     RQQLDNTYVQTADGAKIPYAEYAKLSKKASPAGEVGREAD"
     gene            2262..3479
                     /locus_tag="ACZPAX_04310"
     CDS             2262..3479
                     /locus_tag="ACZPAX_04310"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_function: GO:0044183 - protein folding chaperone
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HIR1113153.1"
                     /translation="MKLKNFHRLAALGLAVGMITALAACGNTAETAESTAETAESTEA
                     ASSEAADAATDETAADAEYAYLADFSFSDAFDENGYLKGVTATDYVTLPDDYADITID
                     ADLGQVSEEDIDNYITSNVLSKFSTTEQVTDRAAADGDTVNIDYVGRIDGVAFDGGDT
                     KGNGADLTLGSGTYVDNFEEQIVGHNPGETFDVTVTFPEDYGNEDLNGKEAVFETTLN
                     YIENTVTPELSDEWVAENLAATMSLNNVDELKAFVKSTMLYDNQASDVYTALHDKVSY
                     ADELPQTALDYYRDVLLYRIYTYAQNYGTDMTTLLSSGMMGATYDSVDAYLDDATDSI
                     KSITQQALLMQAVAEKMGFKCDTDTMNADFGRYYGTTDPSQYVSAYGENYIKMNVLQS
                     DVMQNLIDNVKYE"
     gene            3590..4519
                     /gene="metA"
                     /locus_tag="ACZPAX_04315"
     CDS             3590..4519
                     /gene="metA"
                     /locus_tag="ACZPAX_04315"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004414 - homoserine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase MetA"
                     /protein_id="HIR1113154.1"
                     /translation="MPLIIPKNLPAYDELQKENVFIMHQERAMSQHIRPLRILILNLM
                     PTKIVTETQLARLLANSPLQVQVTFLQTATHSTTHTAPEHMKAFYKTFDEIKNERFDG
                     MIITGAPIEDLDYEDVDYWEELCRIMDYTKENVYSTIHLCWGALAGLYYHFGIPKVHL
                     DKKMFGVFEHRVTRPSNPLVRGFDEVFYAPHSRWAGLDRAAIDACGDLRILAESDVAG
                     PMLLSTESGRQIFVIGHPEYDKYTLDKEYKRDVAAGKPINIPCNYYPDDDPSRDPLFR
                     WRAHGYLLYTNWLNYYVYQDTPYDLNHLEALEK"
     gene            4586..5374
                     /locus_tag="ACZPAX_04320"
     CDS             4586..5374
                     /locus_tag="ACZPAX_04320"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015536677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR1113155.1"
                     /translation="MPKISACIVAYCDYDEVCAAVRSILHYTPTPDLTLYVVDNASPD
                     GCGKQLAETDFGDARVTVLTLSENVGFGRGHNAVLDRLTSEVHFILNPDIVLTENILE
                     PMAAWLLAQEGAAMATPQLHYPDGRLQHLPRRKPTPWLLLARQLAPKFDGIFKKADDH
                     YTMQDEDLTVPRRIAFCTGSFMAVRTNVFKEIGGFDPAYFMYVEDADLTQKVLQKGTV
                     WLTPQFSAIHAWHRAPMRDAGKFKMQLVSMGRYFKKWGCGKGTF"
     regulatory      5455..5544
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF01750"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ZMP/ZTP riboswitches appear to regulate genes
                     involved in one-carbon metabolism; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="5-aminoimidazole-4-carboxamide
                     ribonucleotide (ZMP)/
                     5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranosyl
                     5'-triphosphate (ZMT)"
                     /db_xref="RFAM:RF01750"
     gene            5593..>6948
                     /locus_tag="ACZPAX_04325"
     CDS             5593..>6948
                     /locus_tag="ACZPAX_04325"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016320442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HIR1113156.1"
                     /translation="MVMLSDIEIAQAAEMKPITEIAAQLGLQSEDVIPYGHYKAKINH
                     KLAKRDKPEGKLILMTAISPTPAGEGKTTTSVGLADAMNALGKKTMLCLREPSLGPVF
                     GIKGGAAGGGYAQVVPMEDINLHFTGDIHAIGTANNLLAAMIDNSIQQGNPLNIDPRR
                     ITWKRCMDMNDRQLRYIVDGLGGKVNGTPREDGFDITVASEVMAVFCLATDLEDLKAR
                     LSRIVCAYTYDGQPVTAGQIGAAGAMTALLKDAIDPNLVQTLEHNPAIIHGGPFANIA
                     HGCNSAIATKLSLKLADYVVTEAGFGADLGAEKFLDIKCRAAGLHPAAVVLVATVRAL
                     KSHGGVAKPDLSKPNAEAVRAGSANLARHIENLQNFGLPVCVAINAFPTDTAEEMDVI
                     YDICAKAGVPCALSEVFAKGSEGGKALAETVLSILDDKAKVNYTYELNAPLTDKIAAV
                     AK"
BASE COUNT         1481 a         2043 c         1952 g         1472 t
ORIGIN      
        1 tttttccgcc tgctaacagc aggttgcaca ccacgctgac caagcaggcc aagttgccgt
       61 aagccgtgcg gacggcgcgg tcagcggtgt tttcgtggtt tttgatgaag atttttatca
      121 gcaggtctat cagcgggaat accccctttt tttcagaaaa aggcagaccg ggtaagggtc
      181 tgcctttttg ggttggtttc tattttgaac cgcagtctgg tgggtcgacg cgggcatcga
      241 ctcctacatt gcaggggcgg attccttatc cgcccgcatc agcggccgcc tttgccggaa
      301 atcactcccg cacgatgcag gcgccgcgct cggggccgac ggagatgtac ttgatcttgc
      361 agccgaccag ctcttccagg cgcttgacgt agttctgggt ggcctggggc agatcctcaa
      421 acttgcgcac gccggtgata tcgcagtgcc agccggggac tttctccaca acaggctggg
      481 catcgtccag cgtcttgccc atcgggaagc gggtcgtggt ggtgccatcg gtcagcttgt
      541 aggcggtgac gatgggcact tcctccatgt agtcgaggac gtccagcagg gtcagtgcca
      601 gttcgtcgca gccctggcac tgcacaccgt agcggcttgc cacagcgtcg aacgggccga
      661 cacggcgggg acgtccggtg gctgcaccgt actcatggcc gtgctcacgc agcgcatcat
      721 tctcagcctc ggtcatagcc agctcggtgg taaaggggcc ctcaccgacg caggaagaat
      781 aggccttcat gatgccgatg gagaggttca gcttatggcc cgggatgccc gcaccgatgg
      841 gcgcataggc ggacacggtg ttggaagaag aggtataggg ataaatgccg aagtcgatgt
      901 cgcgcagtgc gcccagctgg gcctcgaaca tgatcttctt gccctcgcgg tcagcctggt
      961 caagatagtc gcccacatcg cagatgtagg gggcaaacag cttcccgtac ttctcgcacc
     1021 aggcccacat ttcctccacg ctgacaggct tctggccgta gatgttcacc atcgtcaggt
     1081 tcttggactc aacgatcgtc tccagacgct tcttgacgct ggcgtccata tgtacgaggt
     1141 cacccatacg cagggtcttt ttcatgtact tgtcgccgta ggagtaggca atgccgcgct
     1201 tggtggagcc aaactgggca ccggtcttgg acaggcgctc ctcctccagg ccgtcctgtg
     1261 ccacatggaa gggcatggtg atggtggcac ggtcagaaat tttcaggttc ttgggggtga
     1321 tttccacacc cttttcggtc agcgaagcaa tttccttttc caggtgctcg gggtcaatga
     1381 ccatgccgcc gcccatgacg cagacgacgt ccgggcgcag gatgccggag gggatcaggt
     1441 tcaggacgaa tttgccgcgc tcgttcttga cggtgtgacc tgcattgtcg ccgccctgat
     1501 agcgcgcaac aatgtcatag tcctggctga gcaggtcgac catgcggccc ttgccctcgt
     1561 cgccccagtt aatgccggta atggaagtaa gcatacgttt ctacctcttt tgtaagtaaa
     1621 taggcatttc agccttttcc ggaatctccg ggatccgctg ccatcaatca ttatagcata
     1681 gctgtcaagg gaattctata gatgttttgt acaagaattt gtgcggattc cgtatgttct
     1741 gtcttccccc ttgggggaag gcagctcgaa gagccggatg aggggcgcgt ttggcgtagg
     1801 aacacgttat gcgcaggcaa ggcagggctg cctctcatta gtccgcttcg cggccaactt
     1861 cccccgcggg ggaagccttt ttgctcagtt tcgcatactc cgcgtaaggt atctttgcac
     1921 cgtcggcggt ctgaacatag gtgttatcca gctgctgcct gaacgaggca cgcatatcgg
     1981 tcaggtactc tttgcgcagg gcatcgcgct cggcggtctc gcgctcggtc aggccgatgg
     2041 attttgcctt tctggcaaga gcgttgatgc ggtctatctt agatttttcc atggggttgt
     2101 ctccttttgt aaaaatagtg tttctattat accacattcc cgccgcattt gcccagtggg
     2161 acctgcgcta ctgtaatttt cacacaattc ccattttact ttttctaaaa agcgtgtata
     2221 atagtaaagt atgcaatttt acaaagaaac aggagaatcc catgaaactg aagaatttcc
     2281 atcgcctggc agcccttggt ctggctgtcg gcatgatcac tgccctggct gcctgcggca
     2341 ataccgccga gactgccgaa tctactgcgg agaccgccga gtccaccgag gcagcctcct
     2401 ctgaggctgc ggacgccgcc accgacgaga ctgccgcaga tgccgagtac gcctacctgg
     2461 cagatttcag cttttccgac gctttcgacg agaacggcta tctcaagggt gtgaccgcca
     2521 ccgattacgt caccctgcct gacgactacg ccgatatcac gatcgatgcc gacctggggc
     2581 aagtcagcga agaggatatc gacaactaca tcacctccaa cgtgctgtcc aaattctcca
     2641 ccaccgagca ggttacggac cgcgcggcag cggacggtga caccgtcaac attgactatg
     2701 taggccgcat cgacggcgtt gcctttgacg ggggcgacac caagggcaac ggcgctgacc
     2761 tgaccttggg cagcggcacc tacgtcgaca actttgagga gcagatcgtc ggccacaatc
     2821 ccggcgagac cttcgacgtc accgtcacct tccccgagga ttacggcaac gaggacctga
     2881 acggcaagga ggccgtcttt gagacgaccc tgaactacat cgagaacacc gtcacacccg
     2941 agttgagcga cgagtgggtt gccgagaacc tggccgccac catgagcctg aacaatgtgg
     3001 acgagctgaa agccttcgtc aagagcacga tgctctacga caaccaggcc agcgatgttt
     3061 acaccgccct gcacgacaaa gtcagctacg ccgacgagct gccccagacc gcactggact
     3121 actaccgcga tgtgctgctg taccgcattt acacctacgc ccagaactac ggcaccgaca
     3181 tgaccacgct gctgtcctcc ggtatgatgg gtgctaccta tgacagtgtg gacgcttacc
     3241 tggacgatgc caccgacagc atcaaatcca tcacccaaca ggcactgctg atgcaggctg
     3301 tggccgagaa gatgggcttc aagtgcgaca ccgacacgat gaacgccgac tttggccgct
     3361 actacggcac gaccgacccg agccagtatg tttccgcata tggcgagaat tacatcaaga
     3421 tgaacgtcct gcagagcgac gtgatgcaga acctcatcga taacgtcaag tacgaataag
     3481 cgtttttgat cgcccccgcg cccccggcag atgccgaggg cgttttttct tgctttttgc
     3541 ggcggaagcc tttataatag aattattcta tagcaaggag ttctccccta tgccgcttat
     3601 cattcctaaa aatcttcctg cctatgatga attgcagaaa gagaacgtct ttattatgca
     3661 tcaggaacgc gccatgagcc agcacattcg gccgctgcgc atcctgattt taaacctgat
     3721 gccgacaaaa atcgtcaccg aaacgcagct ggcgcggctt ttggccaaca gccccttgca
     3781 ggtgcaggtc acgtttttgc agacggctac ccacagcacg acccatacgg cacccgagca
     3841 tatgaaggcg ttttacaaga cgttcgatga aatcaaaaat gagcgtttcg acggtatgat
     3901 catcaccggc gcgcccattg aagatttgga ttacgaggat gtggactact gggaagagct
     3961 ctgccgcatc atggattaca ctaaagaaaa cgtttactct accattcatc tgtgctgggg
     4021 tgcactggca ggtttgtatt accacttcgg cattcccaag gtccatctgg acaaaaaaat
     4081 gttcggcgtg ttcgagcacc gcgtcacccg gccctccaat ccgctggtgc gcggttttga
     4141 cgaggttttt tacgccccgc acagccgctg ggccgggctg gaccgcgccg ccatcgatgc
     4201 ctgcggcgat ttacgcattt tggcagaaag tgacgtagcc gggccgatgc tgctttccac
     4261 cgagtccggg cgtcagattt ttgtcatcgg ccaccccgaa tacgacaaat acacgctgga
     4321 caaggagtac aagcgggatg ttgcggcagg caaacccatc aacattccct gcaactacta
     4381 ccccgacgac gaccccagcc gcgacccgct gttccgctgg cgcgcgcacg gatatcttct
     4441 gtacacgaac tggctgaact actatgtcta ccaggatacg ccgtatgatt tgaaccacct
     4501 ggaagcattg gaaaagtaat agtctactcc gtaggggtcg atgcttgcat ccgcccttac
     4561 aaggtttaac ggtaaggagc accttatgcc caaaatcagc gcctgcattg tggcgtactg
     4621 tgattatgac gaggtctgcg cggcggtgcg cagcatttta cactacaccc ccaccccgga
     4681 tctcaccctg tacgtggtgg ataacgccag ccccgacggc tgcggaaagc agctggcgga
     4741 aactgatttt ggcgacgccc gcgtaaccgt gctgacgctg tcggaaaatg tcggttttgg
     4801 ccgagggcac aacgctgtgc tggaccgcct gaccagcgag gtgcatttta tcctgaaccc
     4861 tgacattgtg ctgaccgaga atattttaga gccaatggcc gcctggctgc tggcacagga
     4921 aggggccgcc atggcaacgc cgcagctgca ctacccggat ggccgtttgc agcacctgcc
     4981 ccgccgtaag cccacaccct ggctgctgct ggcacggcag ctggctccga aatttgacgg
     5041 catcttcaaa aaagcggacg accactacac gatgcaggac gaggatttga ccgtgccccg
     5101 ccgcattgcg ttctgcaccg gcagctttat ggccgtgcgc accaatgtat tcaaagaaat
     5161 cggcgggttt gaccccgcgt atttcatgta tgtcgaggac gccgacctga cccagaaagt
     5221 gctgcaaaag ggcactgtct ggctgacacc gcagttttcc gccatccatg cctggcaccg
     5281 cgccccgatg cgcgacgcgg gcaagttcaa gatgcagctg gtgagcatgg ggcgatattt
     5341 caaaaaatgg ggttgcggaa aagggacgtt ttgattcttt ggttcctcca aaggggatgc
     5401 ttttttgccg tagactcttg acaactgttt ttgtctgtgg tacatttaac ccatagcagg
     5461 gccgctgacc gacggatttt gtggggatga ccccagggga tgcggcgcaa aacctgcgtt
     5521 aagccgaccg tctgggctgc atacactggt gtatggtgtg tgcggccctt ttatttttac
     5581 aaaaacggag gaatcgttat gctgagcgac atcgaaattg cacaggccgc cgaaatgaag
     5641 cccatcaccg aaattgcagc ccaactgggc ctgcagagtg aggacgtcat cccctacggc
     5701 cactacaagg ccaagatcaa tcataaactg gccaagcgcg acaagcccga gggcaagctt
     5761 attttgatga ccgccatcag ccccaccccc gccggtgagg gcaagaccac caccagcgtc
     5821 ggcctggcag acgccatgaa cgccctgggc aaaaagacga tgctctgcct gcgtgagccg
     5881 tccctcggcc ctgtgttcgg catcaagggc ggcgcggccg gcggcggcta tgcccaggtc
     5941 gtgcccatgg aggatatcaa cctgcacttt accggcgata tccacgccat cggcaccgcc
     6001 aacaatctgc tggcggccat gatcgacaac tccatccagc agggcaaccc gctgaacatc
     6061 gacccccgcc gcatcacctg gaagcgctgc atggatatga acgaccgcca gctgcggtat
     6121 atcgtggatg gcctgggcgg caaagtcaac ggaacgcccc gtgaggacgg ctttgatatc
     6181 accgttgcca gcgaagtcat ggcagtgttc tgcctggcca ccgacctgga agatttgaag
     6241 gcacgcctgt cccgcattgt ctgtgcctac acctacgacg gccagcctgt cacggccggg
     6301 cagatcggcg cagcgggcgc gatgaccgcc cttctgaaag acgccattga tccgaacctt
     6361 gtccagacgc tggagcacaa ccccgccatc atccacggcg ggccgttcgc caacattgcc
     6421 cacggctgca acagtgccat tgccaccaag ctgagcctga agctggctga ctacgttgtg
     6481 accgaggcgg gctttggcgc agatctgggc gcggagaagt ttctggatat caagtgccgc
     6541 gctgccgggc tgcaccccgc cgccgttgtg ctggtggcaa ccgtgcgtgc gctgaaatcc
     6601 cacggcggcg tggcgaagcc tgatttgagc aagcccaacg ccgaggctgt gcgggcgggt
     6661 tctgccaacc tggcacgtca cattgaaaat ttgcagaact tcggcctgcc ggtctgcgtt
     6721 gccatcaacg ccttccccac cgacacggcg gaggaaatgg acgtcattta cgacatctgc
     6781 gccaaggccg gtgtgccctg cgccctgagt gaggtctttg ccaagggcag tgagggtggc
     6841 aaggccctgg ccgagacggt gctttccatc ctggatgaca aggccaaggt caactacacc
     6901 tatgagctga atgcaccgtt gaccgataag attgcggcgg ttgccaaa
//
LOCUS       DBMDSQ010000081         6945 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_2841_length_6945_cov_2.188486, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000081 DBMDSQ010000000
VERSION     DBMDSQ010000081.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 6945)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6945)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6945
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2841_length_6945_cov_2.188486"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..562)
                     /locus_tag="ACZPAX_04330"
     CDS             complement(<1..562)
                     /locus_tag="ACZPAX_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015537612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HIR1113157.1"
                     /translation="MAEKRDYYEVLGIGKNATDAEIKSAYRKLAKKYHPDLNPGDKDA
                     EEKFKEVNEANDVLSDPDKRKRYDQFGFAGVDPNYGAGQPGGGFGGGFGGGFGAGGVD
                     LGDIFGDLFGGGGFGGFGGSSRANPNAPRKGHDIQANVILTFEEAAHGCAKKVTLNRQ
                     QTCPDCNGNGCAPGTSPETCTECGGRG"
     gene            complement(815..2647)
                     /gene="dnaK"
                     /locus_tag="ACZPAX_04335"
     CDS             complement(815..2647)
                     /gene="dnaK"
                     /locus_tag="ACZPAX_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005943578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HIR1113158.1"
                     /translation="MSKIIGIDLGTTNSCVAVMEGGEPVVIANAEGARTTPSVVGFTK
                     TGERLVGQVAKRQAITNPENTISSIKRKMGTAEKVHAGGKDYTPEEISAMILSKLKAD
                     AEAYLGEPVTEAVITVPAYFNDSQRQATKNAGTIAGLNVKRIINEPTAASLAYGIDKE
                     ADQKIMVYDLGGGTFDVSIIEMGDGVTEVLATNGDTHLGGDDFDERIINWMAEDFQRE
                     NNIDLRKDKMAAQRLKEAAEKAKIELSSATTTNINLPFITADANGPKHLDMTLTRAKF
                     NELTADLVERTMTPVRKALADAGLKASDLAKVLMVGGSTRIPAVYDAVKKELGCEPFK
                     GINPDECVAVGASIQGGVLQGDVKGLLLLDVTPLSLGIETLGGVCTKIIDRNTTIPTK
                     KSQIFSTAADNQPSVEVNVLQGEREFARDNKSLGTFHLDGIAPAPRGVPQIEVTFDID
                     ANGIVHVSAKDLGTGKEQSITITASTNMSKDDIDKAVKDAEQYAAEDKKRREEVDVRN
                     NADQMVFQTEKALGEFGDKVSAEEKSEVETKLSALKEALKSGSIDDIKAKQDDLQKAF
                     YAISEKVYQQAAQAQGQQPGAQPGPDAGSQPKDDGAVDADFHEV"
     gene            complement(2729..3322)
                     /locus_tag="ACZPAX_04340"
     CDS             complement(2729..3322)
                     /locus_tag="ACZPAX_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015564460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HIR1113159.1"
                     /translation="MSHEKETKQTPAAETKAPETEAKAPEQEAPKAEKPEAKHEGKHH
                     HETAALQAKLDEAEKKNEELKNQLLRTAAEYDNYRKRSQREADQKFNDGVSHAVTQIL
                     GILDTLDMAANAACTDENYKKGVMMTLDKAAKALETLNITEIEALNQPFDPNFMNAVS
                     QAPAAEGQESGSVTQVFQKGYKIGDKIIRHAMVVVAE"
     gene            complement(3344..4357)
                     /gene="hrcA"
                     /locus_tag="ACZPAX_04345"
     CDS             complement(3344..4357)
                     /gene="hrcA"
                     /locus_tag="ACZPAX_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HIR1113160.1"
                     /translation="MAMDARKQRILEAIVALYANGGEPVGSGLLANYFDMALSSATLR
                     NEMAALTKLGLLEQPHTSAGRVPSAQGYRYYLDHLIDAPKASALPDADRRHIDDLFAA
                     MDAEPEKLVPAAARSLADLTGCAAAATTPQAPDLCIAHFEVVQVGRYSAAVLAVTSAG
                     GVRTRVARVDTGLSRDDAANIAQLLNRGLTFVAPQDLSPMLMASLVLAAGERLSPVIM
                     AAQALVTTGPQACLEGAQYLAKMPDVRQNLGTLLEIFSDNAAATELIRPEGSRVTATL
                     GCDLDPAMPGCCIVSKRYLAGGGLTGSVALIGSTRMEYERLLPILDYFAAKLGQSMAG
                     QGQ"
     gene            complement(4637..5170)
                     /locus_tag="ACZPAX_04350"
     CDS             complement(4637..5170)
                     /locus_tag="ACZPAX_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015564462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation T C-terminal
                     domain-containing protein"
                     /protein_id="HIR1113161.1"
                     /translation="MRATGIVRRIDELGRVVIPKEIRRTQRIHSGDALEIFTDGKGEV
                     VFKKYSPLAELGEPAAAYADVLARRLGCGVAVCDREKIVAAAGAGRSELLGEPLPPEL
                     DALLARRKLYTAPEEPLHRVALGSRWLLCAAPVLVHGDIEGGVLLPGMRRDAVPDPEA
                     IRAAATAAEFLARWMGE"
     gene            5238..5711
                     /locus_tag="ACZPAX_04355"
     CDS             5238..5711
                     /locus_tag="ACZPAX_04355"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005934661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR1113162.1"
                     /translation="MDLLQTTLCYLEKDGAYLMLHRVKKKNDVNHDKWVGVGGKFEPG
                     EDALTCALREVREETSLTMHTPVYRGIVDFYCDPWPAERMHLYTCTDFSGVMTDCDEG
                     CLEWVDKNAVQDLPIWQGDKIFFKLLAEDAPFFHLELTYEGDVLVKAVLDGEGLL"
     gene            complement(5899..>6945)
                     /locus_tag="ACZPAX_04360"
     CDS             complement(5899..>6945)
                     /locus_tag="ACZPAX_04360"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HIR1113163.1"
                     /translation="GSTNAVETDLNKGAEGTLPEQTVTVDQIKVTATPAPTDAPTPEP
                     TEAPASDANSNSSEPTPTEAPTQAPQPDNSTGTEGETINVTMNGTAQTMDLVRCLAMV
                     AQNELGPNAPAEAYKAQCVATHCWILSQSGYPSVLGAEPGAAALAAAQEVAHVLVTYN
                     GQVCFTPYFASASTGTASSAEVWGGERAWLQAVDSPYDQQVSSNWNTNGNSSGTARFS
                     RETLENRIRDELGIDLAGVDPNSWFTIKSANQYGWVAKIQVGPDGNSETVSGRWFREN
                     LLARQSVDGRSLRSQCFTVSYDADLDCFIFDVYGYGHGCGMSQWGAIGYARNGWSYQD
                     ILTHYFVGTTITTY"
BASE COUNT         1151 a         2103 c         2182 g         1509 t
ORIGIN      
        1 agccgcggcc gccgcattcg gtacaggtct cggggctggt gccgggggca cagccgttgc
       61 cgttgcagtc cgggcaggtc tgctggcggt tcagcgtcac ctttttggcg cagccgtggg
      121 ccgcttcctc aaaggtcagg atgacgttgg cctggatatc gtggcccttg cgcggggcgt
      181 tggggttggc gcggctggaa ccgccgaaac cgccaaagcc accgccgccg aacaaatcgc
      241 cgaagatatc gcccaggtca acaccgcctg cgccgaaacc gccgccaaag ccgccaccga
      301 agccgccgcc gggctggccg gccccatagt tgggatcgac cccagcgaaa ccgaactggt
      361 catagcgctt gcgcttgtcc gggtcggaaa gaacatcgtt ggcctcgttg acctccttga
      421 acttttcctc ggcgtcctta tcgccggggt tcaagtcggg gtggtacttt ttggccagct
      481 tgcggtaggc gcttttgatc tcggcatcgg tggcgttttt gccgatcccc agcacctcgt
      541 agtaatcacg tttttctgcc atagaatcta tcccctctat ttatgggttg tgaaaaggct
      601 tcccgctgag gggaagctgt ccgcgcagcg gactgataag gtgggaactg ccggcgattg
      661 cccataaaca ggctgccacg gcagcgccgc ccctcatccg gcctgcggcc accttccccc
      721 gaggggggaa ggctttattt taactttctc tataaccccc aagggccgcc gccggggtag
      781 cccagcggcg gccttattat gggttagtta cgcatcaaac ctcgtggaag tcggcgtcaa
      841 cggcaccgtc atccttgggc tggctgccgg cgtcggggcc gggctgggca ccgggctgct
      901 ggccctgtgc ctgggcggcc tgctggtaga ccttctcgct gatggcgtag aaagccttct
      961 gcaggtcatc ctgctttgcc ttgatgtcat cgatgctgcc ggacttcagg gcctccttca
     1021 gggcggagag cttcgtctcg acttcgctct tctcctcagc ggagaccttg tcgccaaact
     1081 cgcccagggc cttctcggtc tggaacacca tctggtcggc gttgttgcgc acatcgactt
     1141 cctcgcggcg cttcttatcc tcagcggcat actgctcggc gtccttgaca gccttgtcga
     1201 tgtcgtcctt gctcatgttg gtggaagcag tgatcgtgat gctctgctcc ttgccggtac
     1261 ccaggtcctt cgcgctgacg tggacgatgc cgttcgcgtc gatatcaaag gtaacttcga
     1321 tctgcggcac accacgggga gccggagcga tgccatccag atggaaggtt cccaggctct
     1381 tgttgtcgcg ggcaaactca cgctcgccct gcaggacgtt gacctcaacg ctgggctggt
     1441 tgtccgcagc ggtggagaag atctggctct tcttggtggg gatggtggtg ttgcggtcga
     1501 tgatcttggt gcagacgccg cccagggtct caatgcccag gctcagcggg gtaacatcca
     1561 gcagcagcag acccttgacg tcgccctgca gaacgccgcc ctgaatggaa gcgccgacag
     1621 caacgcactc atcggggttg atgcccttga acggctcgca gcccagttcc ttcttgacag
     1681 cgtcgtagac ggcggggata cgggtggagc cgccgaccat cagcaccttg gcgaggtcgg
     1741 aggccttcag gccggcgtcg gccagagcct tgcggacggg ggtcatggtg cgctcgacca
     1801 ggtcagcggt cagctcgttg aacttggcgc gggtcagcgt catatccagg tgcttggggc
     1861 cgttggcatc ggcggtgatg aagggcaggt tgatgttggt ggtggtggcg ctggacagct
     1921 cgatcttagc cttctcagcg gcttccttca aacgctgggc agccatctta tccttgcgca
     1981 ggtcgatgtt gttctcgcgc tggaagtcct cggccatcca gttgatgatg cgctcatcga
     2041 agtcatcgcc gcccaggtgg gtatcaccgt tggtggccag aacctcggta acgccgtcgc
     2101 ccatctcgat gatggagaca tcgaaggtac cgccgcccag gtcgtagacc atgatcttct
     2161 ggtcggcttc cttatcgatg ccgtaggcca gagaagcagc ggtcggctcg ttgatgatac
     2221 gcttgacgtt caagccggca atggtgcctg cgttcttggt agcctgacgc tggctgtcgt
     2281 tgaagtaggc ggggacagtg atgacggcct cggtgacagg ctcgcccagg taagcctcgg
     2341 cgtcagcctt cagcttgctc aggatcatag cgctgatctc ttcgggggtg tagtccttgc
     2401 cgccggcgtg gaccttctcg gcggtgccca tcttacgctt gatggaggag atggtattct
     2461 cggggttggt gatggcctga cgctttgcga cctggccgac cagacgctcg ccggtcttgg
     2521 tgaagccgac gacagacggg gtggtgcggg cgccctcggc gttcgcgatg acgacaggct
     2581 cgccgccttc cataacagcg acgcagctat tggtagtacc aaggtcaata ccgatgattt
     2641 tgctcataat aaaaactccc ttcaaatttg ctttgaacag tagtgaatgt caatttatat
     2701 ggaaacgaat cttttgattc ggttcgcctt attctgcgac gacgaccatc gcgtggcgga
     2761 tgatcttgtc accgatcttg tagcccttct ggaaaacctg cgtgacgctg ccgctctcct
     2821 ggccctcggc ggcgggggcc tgcgaaacgg cgttcatgaa gttggggtca aacggctggt
     2881 tcagcgcttc gatctcggtg atgttcagcg tttccagggc cttggcggcc ttatccagcg
     2941 tcatcatgac gcccttcttg tagttctcgt cggtgcaggc ggcgttggca gccatatcca
     3001 gcgtgtccag gatgcccaaa atctgggtca cagcgtggga cacaccgtcg ttgaacttct
     3061 ggtcggcctc gcgctggctg cgcttgcggt agttatcata ctcggcggcg gtgcgcagca
     3121 gctggttttt cagctcctcg ttctttttct ccgcctcgtc cagcttagcc tgcaaggccg
     3181 ctgtctcgtg gtggtgctta ccctcgtgct ttgcctcggg cttctcggcc ttgggggcct
     3241 cctgctcagg ggccttggcc tcggtctcag gcgccttggt ctctgcggcg ggcgtctgct
     3301 tggtttcttt ttcgtggctc attgcgggtc tccttctttc caattattgg ccctgcccgg
     3361 ccatactctg ccccagcttt gcggcaaagt agtcgaggat cggcagcagc cgctcgtatt
     3421 ccatgcgggt ggaaccgatg agcgccaccg agcctgtcag cccgccgccc gccagatacc
     3481 gcttgctgac gatgcagcag cccggcatgg ccgggtccag gtcgcagccc agggtggccg
     3541 taacgcggct tccctcgggg cggatcagct cggtagcggc cgcgttgtcg gagaaaatct
     3601 ccagcagcgt gcccaggttc tggcggacat ccggcatctt tgccaggtat tgtgcgcctt
     3661 ccaggcaggc ctgcgggccg gtggtgacaa gcgcctgcgc ggccatgatc acggggctga
     3721 gccgctcacc tgcggccagc accaggctgg ccatcagcat ggggctgagg tcctgcgggg
     3781 ctacaaacgt aagcccccgg ttgagcagct gcgcgatgtt ggcggcgtcg tcccgggaca
     3841 ggccggtatc gacgcgggcc acgcgggtac gcaccccgcc tgcgctggtg actgccagca
     3901 ccgccgcgct gtaccgcccg acctgcacaa cctcaaagtg ggcgatgcag agatccgggg
     3961 cctggggcgt ggtggcggca gcggcgcagc cagtcaaatc agccaaactg cgggctgcag
     4021 cgggcacgag cttttccggc tcggcatcca tggcggcgaa caggtcatcg atgtggcggc
     4081 ggtcggcatc cggcagggcg ctggccttcg gcgcatcgat caggtggtcg aggtagtagc
     4141 gatagccttg ggcactgggc acacggcccg cactggtgtg gggctgttcc agcaggccca
     4201 gcttagtcag cgccgccatc tcgttgcgca gcgttgcact ggacagtgcc atgtcaaagt
     4261 agtttgccag caggcccgaa ccgaccggtt cgccgccgtt ggcatacagg gcaacaatgg
     4321 cttccagtat acgctgcttg cgcgcgtcca tggccatgct gctcatctcc tttggtttgc
     4381 tttcggtttt gtttagcact ctttatctct gagtgctaag ttaattatat tcccgttttg
     4441 caaactgtca aggggttttt ccgatttttt tgtaagttta acagtttcgt cacaatcacg
     4501 gcagagttct tacctattat atatgtaggg gccggttatg cccggcctgc acttgtacgg
     4561 aaaacggtcg cttgcgggaa agctgagggc cgggcacgcc cggcccctac atggcggtgt
     4621 gcattcagac accccgtcac tcccccatcc atcgcgctaa aaactcggcg gcggtggcgg
     4681 cggcgcggat ggcctcgggg tcgggtacgg catcccggcg catgcccggc agcagcacgc
     4741 cgccctcaat atcgccatgc accaaaaccg gcgcggcgca cagcagccag cggctgccca
     4801 acgcgacccg gtgcagcggc tcttccggtg cggtgtacag cttgcggcgg gccagcaggg
     4861 cgtccaactc aggcggcagc ggctcgccca gcagctcact tctgcccgcc ccggcggcgg
     4921 ccacgatctt ttcccggtca cagacagcca caccacagcc cagccgccgg gccagcacat
     4981 cggcgtaggc ggcggcaggc tcgcccagct ctgccagcgg cgaatacttt ttaaagacaa
     5041 cttccccttt gccatcggta aaaatttcca gtgcgtcgcc cgagtggatg cgctgggtac
     5101 ggcgtatctc cttcgggatg accacccggc ccaattcatc gatgcgccgc acaattcctg
     5161 tcgctctcat acagaatcct ccctggtttt ttatggtagt atctgcggat tcatacaaaa
     5221 tatgtaagga gttccctatg gatttactgc aaaccaccct ttgctatctc gaaaaagacg
     5281 gcgcgtacct gatgctgcac cgcgtcaaaa agaaaaacga cgtcaaccac gacaagtggg
     5341 tcggcgtcgg cggcaagttt gagccgggcg aggatgcgct gacttgcgcc ctgcgcgagg
     5401 tgcgcgaaga gaccagcctg acgatgcaca cgcctgtcta tcgcggcatc gtggattttt
     5461 actgtgaccc ctggcccgcc gagcggatgc acctgtacac ctgcacggat ttttccggcg
     5521 tgatgacaga ctgcgacgag ggatgtttag agtgggtgga caagaatgct gtgcaggatt
     5581 tgccgatttg gcagggggat aaaattttct ttaagctgct ggctgaggat gcgccgtttt
     5641 tccacctgga attgacgtat gagggggacg tgctggtgaa agcggtgctg gatggggagg
     5701 ggttgctata accaccgcgc tgcggaatcc cccagttacc ttcggtgaca gcccccttaa
     5761 caaggggcct ttaaagcctc ccttgtcaaa gggaggtggc cgagcgaagc gaggtcggag
     5821 ggattcccgc cgtgcggtgg atgttactgc caaaaaagag cgtgtgccat ttcttgcaca
     5881 cgctcttttt ctgtactatc aatatgtcgt aatcgtcgta ccaacgaaat aatgcgttaa
     5941 aatatcctga tagctccagc cgttgcgggc gtaaccgatg gcaccccatt ggctcatgcc
     6001 gcagccgtgg ccgtaaccgt acacatcaaa gataaagcaa tccagatcgg cgtcatagct
     6061 gaccgtgaag cactggctgc gcaggctgcg gccatccaca ctctggcggg ccagcaggtt
     6121 ttcccggaac cagcggccgc tgaccgtctc gctgttgcca tcggggccga cctgaatctt
     6181 ggcgacccag ccgtactggt tggcgctctt gatggtgaac cagctgttgg ggtccacgcc
     6241 tgccaggtcg atgcccagct cgtcgcggat gcggttctcc agcgtctcgc gggagaagcg
     6301 ggccgtgccg ctggaattgc cgttggtgtt ccagttgctg gaaacctgct ggtcgtaggg
     6361 gctgtccacg gcctgcagcc aagcgcgttc accaccccag acctcggcag acgaggctgt
     6421 acccgtgctg gcagaggcga aatacggcgt gaagcagacc tgcccattgt aagtcaccag
     6481 cacatgggca acctcctggg cggcggccag cgcagctgcg cccggctctg cacccagcac
     6541 cgacggatag cccgactggc ttaaaatcca gcagtgggtc gccacgcact gggctttgta
     6601 ggcttcggcg ggggcgttgg ggccaagttc gttctgggcc accatcgcca ggcagcgcac
     6661 caggtccatc gtctgggccg tgccgttcat cgtcacgttg atggtctcgc cctcagtgcc
     6721 ggtgctgttg tcgggctgcg gggcctgggt cggcgcttcc gtcggggtcg gttcgctgct
     6781 gttactgttg gcgtccgacg ccggggcctc ggtaggttct ggcgtcgggg cgtcggtagg
     6841 ggccggcgtg gccgtcacct tgatctggtc cactgtgacc gtctgctcgg gcagcgtgcc
     6901 ctcggcaccc ttgttcaggt ccgtctccac agcgttcgtc gagcc
//
LOCUS       DBMDSQ010000082         6934 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_2845_length_6934_cov_1.823211, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000082 DBMDSQ010000000
VERSION     DBMDSQ010000082.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 6934)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6934)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6934
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2845_length_6934_cov_1.823211"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..246
                     /locus_tag="ACZPAX_04365"
     CDS             <1..246
                     /locus_tag="ACZPAX_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002610643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HIR1113164.1"
                     /translation="LFILVAGVLGTARIILQDALNALGYSTNTLDGIFGKATENALKA
                     CQRRFSLTADGVCGCASWRKIAEATVGIGKTATVIDA"
     gene            304..879
                     /locus_tag="ACZPAX_04370"
     CDS             304..879
                     /locus_tag="ACZPAX_04370"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HIR1113165.1"
                     /translation="MDMMEFLRSRRTYRRFAQRAVAPEILTEAVDAARIASCGANRQT
                     VRYCIVESPAAVAAVQPLVHWAAYLPREQGTPQPDEQPTAFIAILQDDRLPGANDVDI
                     GLALGSLTAAAWAHGVGSCIMGAIDRPALTKLLGLPEGLRLCYMVALGYPTHESHLVE
                     IQDGSVKYYLDENRNYCVPKRGMEEILIKTL"
     gene            complement(1003..2571)
                     /locus_tag="ACZPAX_04375"
     CDS             complement(1003..2571)
                     /locus_tag="ACZPAX_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HIR1113166.1"
                     /translation="MKQWLKDAVFYEIYPQSFYDTNGDGIGDLQGIIAKLDYIKGLGC
                     NALWINPCFDSPFKDAGYDVRDYKKIAQRYGTNEDAAALFKAAHEKGIRVLFDLVPGH
                     TSEEHEWFKASCRPEKNEYSDRFIWTDSCFASGDGMPFIGGETERNGTYILNFFKCQP
                     ALNYGYGKINQKWQMPTDDPACVATVEAMKDVMRFWLDMGCDGFRVDMASSLVKNDTK
                     NKKYTCKIWRNIRDMLDVEYPEAALIAEWNGPRMSLKNGFDMDFYLEWGGNGYSWLMR
                     NYDGTMDSNPHNIGKAYFCKNSGTGIDKFLDEYLPTYKATHKDGLWCFITCNHDTIRP
                     SAGLTVEELRLAYATLFTLPGAPFVYYGDEIGMRYLPLPTKEGGYFRTGSRTPMQWDD
                     SANHGFSTADAKDIYLPVDTAEDAPTVAAQADDPASLLNSVKSLLAFRHAHKDLNSDA
                     PFKVLYAPKAKDDYRPFVWQRGDLICAVNPAGVSIELPLELAEDLKIQYTIGQAEIVN
                     DTLSLGAQSFVILG"
     gene            2926..4656
                     /locus_tag="ACZPAX_04380"
     CDS             2926..4656
                     /locus_tag="ACZPAX_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008117296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HIR1113167.1"
                     /translation="MNTLVIVLIAAVVLVCAYTGYGRWLAKTWGVDPNAKTPAVRLED
                     GKDYVPTNGWTVFAHQFSSIAGAGPVTGAIQAAAFGWLPVLLWVLIGGVFFGAVTDFG
                     ALYASVKNDGKSMGLLIEKYIGKTGRKLFLLFCWLFCGIVIAAFADMVAGTFNAFDAD
                     GAQVEAAFTNGAAGMVSIMFMVFAVVFGFIQKKFNFSGWKEAVIGIAFIVLSFVIGMN
                     APITLGKAGWSYITFVYIFFAAVLPMWMLKQPRDYMTTFMFGAMIAGAVIGLVVAHPT
                     MNLPVFTGFNNEKLGTMFPILFVTVACGAVSGFHSLVSSGTSSKTVENEKDMLKVGYG
                     AMVLESLLAVLALCVAGAAAAADGTPAAGTPFQIFSRGVAGFFEMFGVPVSIATVFMT
                     MCVSALALTSLDAVARIGRMSFQELFSVDDMEHAEGWRKLFCNVYFSTFITLAFGFLL
                     TQIGYANIWPLFGSANQLLSALVLATLCVFLKVTGRNNKMLFPPLVIMLCVTFTALVQ
                     RLIAMVKAISAAASTAIPAGETTWGAVFIANGLQLILAILLIVLGLNIVFHSVKSYKN
                     SEKDSEKVAA"
     gene            4840..5739
                     /locus_tag="ACZPAX_04385"
     CDS             4840..5739
                     /locus_tag="ACZPAX_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HIR1113168.1"
                     /translation="MKLKNAMALGLASVLAVGLTACGSSADTSAAESTVAAADAAGAP
                     DGDGDPTTLTVAMECAYAPYNWTQSDDSNGAVEIRGSSDYAYGYDVMMAKKIGEALGQ
                     KVQIVKLDWDSLIPAVMSGDVDCVIAGQSITAERAAQVDFSDPYYYASIITLTKKDSA
                     YANAASVADLAGATATSQLGTIWYDTCLPQIENANILPAQETAPAMLVALNSGACDIV
                     VTDRPTGQAALVAYPDFTLLDFGGGDGDFQVSDEDINIGISMKKGNTALKDAINEVLA
                     TMTTDDYNALMDEAISVQPLSDN"
     gene            5807..6631
                     /locus_tag="ACZPAX_04390"
     CDS             5807..6631
                     /locus_tag="ACZPAX_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HIR1113169.1"
                     /translation="MQDFFANCATVLQKYGFSYLRGAGTTLLLALVGTFFGCLIGFAV
                     GALQTIPVDKQRDPVWKRVLLKILHIFLRCYVELFRGTPMIVQAVFIYYGLLQVFGIK
                     VGMWQAGFFIVSINTGAYMAETVRGGIVSIDPGQTEGAKAIGMNHWQTMIHVILPQAL
                     RNIIPQIGNNFIINIKDTSVLSVISITDLFFVHKSVVGALYTYFESAAIVMVIYLTMT
                     LFASYLLRLWEKALDGPQNYDLNTTDSLAYTSGMYNAPDPNHESQNLDMKGSAAHV"
     gene            6624..>6934
                     /locus_tag="ACZPAX_04395"
     CDS             6624..>6934
                     /locus_tag="ACZPAX_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR1113170.1"
                     /translation="MSEPVIEVRHLSKSFGTHVVLRDVDFTVNAGDVTCIIGASGSGK
                     STLLRCINLLETPTNGKVLFHGNDITAKGSDPCAYRAKVGMVFQSFNLFNNMTVLDNC
                     TA"
BASE COUNT         1361 a         2057 c         1991 g         1525 t
ORIGIN      
        1 ctctttatac tggtggctgg cgtgctgggc accgcccgca tcatcctgca ggacgcgctg
       61 aacgcgctgg ggtattccac caacacactg gacggcatct tcggaaaggc gaccgagaac
      121 gcgctgaaag cctgccagcg gcggttcagc ctgaccgccg acggcgtatg cgggtgcgcg
      181 agctggcgga agatcgccga agcgacggtg ggcatcggga aaacggcaac ggtgattgat
      241 gcgtgaacag gaaacgtgtt ataatgaaaa tacgaactct atataataag gaagtgcccc
      301 caaatggata tgatggaatt tctgcgcagc cgccgcacct accggcggtt tgcacagcgg
      361 gctgttgcgc ccgagattct gaccgaagcc gttgatgcgg cccgcattgc cagctgcggc
      421 gcgaaccgcc agaccgtgcg gtattgtatt gtggaaagcc ccgccgccgt ggccgccgtg
      481 cagccgctgg tgcattgggc cgcctacctg ccccgcgagc aaggcacgcc gcagcccgac
      541 gagcagccca ctgcgtttat cgccatcttg caggatgacc gtctgcccgg tgcgaacgat
      601 gtggacatcg gcctggcgct gggcagcctg accgctgccg cctgggcaca cggcgttggc
      661 agctgcatca tgggggccat cgaccggccc gcgctgacca agctgctggg tctgcccgag
      721 ggactgcggc tgtgctatat ggtcgcactg gggtatccga cgcatgagag ccacctggtg
      781 gaaatccagg acggcagtgt gaagtattat ctcgatgaaa accgcaacta ctgcgtaccg
      841 aagcgcggga tggaggaaat tttaattaag acattataat agtatagcaa aacccgcaat
      901 cccggttata acatcgagat tgcgggtttg ttttgttgca gggggcaaat ctcatatccg
      961 cccgtggatg tttataggaa aattgcaggg cagtttttca ttttacccca aaatcacaaa
     1021 actctgtgcc cccagagaca gggtatcatt gacgatctcg gcctggccga tggtgtactg
     1081 aatcttcaga tcctcggcca gttccagcgg cagctcaatg gagacgcctg ccgggttgac
     1141 ggcgcagatc aggtcgccgc gctgccagac aaagggacgg tagtcgtcct tggccttggg
     1201 tgcgtacagt accttgaacg gcgcatcgga gttcaggtcc ttgtgggcat ggcggaaggc
     1261 cagcagcgac ttgacgctgt tcagcaggct ggcggggtca tcggcctggg cggccacggt
     1321 gggggcatcc tcggcggtgt ccacaggcag gtagatatcc tttgcgtcag cggtggagaa
     1381 gccgtggttt gcgctgtcat cccactgcat cggggtacgg gagcctgtgc ggaagtagcc
     1441 gccctccttg gtgggcagcg gcaggtagcg cataccgatt tcatcgccgt agtagacaaa
     1501 cggcgcacct ggcagcgtaa agagggtcgc ataggccagg cgcagttcct ccacagtcag
     1561 gccggccgag gggcggatcg tgtcgtggtt gcaggtgata aagcaccaca gaccgtcctt
     1621 gtgggtggcc ttgtaggtgg gcagatactc gtccaggaac ttgtcgatac cggtgccgga
     1681 gttcttgcag aagtacgcct tgccgatatt gtgcgggttc gagtccatcg taccgtcata
     1741 gttgcgcatc agccagctgt acccattgcc gccccactca aggtagaagt ccatgtcaaa
     1801 gccgtttttc agcgacatgc gcgggccgtt ccactcggcg atgagcgcgg cctcggggta
     1861 ctcgacgtcc agcatatccc ggatattacg ccagatcttg caggtgtatt tcttgttttt
     1921 ggtgtcgttc ttgaccaggc tggacgccat atcaacgcgg aagccgtcgc agcccatatc
     1981 cagccagaat cgcatgacgt ccttcatggc ctccaccgtt gccacgcagg cggggtcgtc
     2041 ggtgggcatc tgccacttct ggttgatctt accgtaaccg tagttcagcg caggctggca
     2101 cttgaagaag ttgagaatgt aggtgccgtt gcgttcggtc tcgccgccga tgaagggcat
     2161 accgtcgccg ctggcaaagc aggaatccgt ccagatgaag cggtcggagt attcgttctt
     2221 ttcgggtcgg cagctggcct tgaaccactc gtgttcctcg ctggtgtggc cggggacgag
     2281 gtcaaacaag acacggatgc ccttctcgtg ggcagctttg aagagggcgg cggcgtcctc
     2341 gttcgtgccg tagcgctggg cgatcttttt gtagtcacgc acgtcgtaac cggcgtcttt
     2401 aaacggcgag tcaaagcaag gattgatcca cagagcgttg cagcccaggc ccttgatgta
     2461 gtccagcttg gcgataatgc cctgcaggtc gccgataccg tcgccgttgg tatcgtagaa
     2521 gctctgggga tagatttcat agaatactgc gtctttgagc cattgtttca tgggggttct
     2581 cctttatata ataagtaggg ggtctaaagc ctccccttga ggacagactc ccctgatgcg
     2641 ggggagtctg cgcatagcgc cggagggcgg agttttcccc aagagggggc ctttggggat
     2701 tccgtggaaa cgtttcattc tctttctata ccttatacta tccccttccg cgcactttgt
     2761 caacggcgca tcttgtacaa aacatgacat aaattttcta cattttgcac caaaaaccta
     2821 catattgcca aattatgaac atcatgttga caaattgcga acagcgcggt aaagtagagg
     2881 gcacaagcga ttgtaaaatc tagtaggaaa gagggatcta ttcaaatgaa tacgcttgta
     2941 atcgttctga ttgccgcggt cgtcctggta tgtgcgtaca caggatacgg tcgctggctc
     3001 gccaagacct ggggtgttga ccccaacgcc aaaactcccg ctgtgcggct ggaggatggc
     3061 aaagactacg ttcccaccaa cggctggact gtgtttgcac accagttctc ttctattgca
     3121 ggtgccggcc ctgtcaccgg tgctattcag gctgctgcct tcggctggct gcctgttctg
     3181 ctctgggtac tgatcggcgg cgtcttcttc ggcgctgtca ctgacttcgg cgctttgtac
     3241 gcttccgtca agaacgacgg taaatccatg ggcctgctga tcgagaagta catcggcaag
     3301 accggccgca agctgttcct gctgttctgc tggctgttct gcggcatcgt cattgctgcc
     3361 ttcgcagaca tggtcgccgg caccttcaac gccttcgacg ctgacggtgc acaggtcgag
     3421 gccgccttca ccaatggtgc agccggcatg gtctccatca tgttcatggt gttcgccgtt
     3481 gtgttcggct tcatccagaa gaaattcaac ttctccggct ggaaagaggc tgtcatcggc
     3541 atcgccttca tcgtgctgtc tttcgtgatc ggcatgaatg cccccatcac cctgggcaag
     3601 gctggttgga gctacatcac cttcgtttac atcttcttcg ccgccgtcct gcccatgtgg
     3661 atgctgaagc agccccgtga ctatatgacc accttcatgt ttggcgctat gattgccggt
     3721 gcggtcatcg gtctggtcgt tgcccacccc accatgaacc tgcctgtttt caccggcttc
     3781 aacaatgaga agctgggcac catgttcccc atcctgttcg tcaccgtcgc ctgcggcgct
     3841 gtctccggct tccacagcct ggtttcctcc ggtacttctt ccaagaccgt tgagaatgag
     3901 aaagatatgc tcaaggtcgg ctacggcgcc atggtgctgg aaagcctgct ggctgttttg
     3961 gccctgtgtg ttgccggtgc tgccgccgct gcggacggca cccctgccgc cggtactccg
     4021 ttccagatct tcagccgcgg cgttgccggt ttctttgaga tgttcggcgt gcccgtctcc
     4081 atcgctaccg tctttatgac gatgtgtgtt tccgctctgg ccctgaccag tctggatgcc
     4141 gtggcccgta tcggccgtat gagcttccag gagctgttca gcgttgacga tatggagcat
     4201 gccgagggct ggcgcaagct gttctgcaac gtctacttct ctaccttcat cactctggcc
     4261 ttcggcttcc tgctgaccca gatcggctat gccaacatct ggccgctgtt cggttccgcc
     4321 aaccagctgc tgagcgcgct ggtcctggct accctgtgcg tcttcctcaa ggtcacgggc
     4381 cgtaacaaca agatgctgtt cccgccgctg gtcatcatgc tctgcgtcac cttcacggct
     4441 ctggtgcagc gcctgattgc catggtcaag gccatctccg ccgctgcgtc caccgccatc
     4501 cctgccggtg agaccacctg gggtgctgtc tttatcgcca acggcctgca gctgattctc
     4561 gcgatcctgc tgatcgtcct gggcctgaac attgtctttc acagtgtcaa gagctacaag
     4621 aacagcgaga aggacagcga aaaagttgct gcgtaatcca aacagcactt gacaaatata
     4681 ccatcttagg gtaatattta tacggccgca atggcgcggc gcaacactgc accattcggc
     4741 agtcataagg catttggccc cggtgatccg cacagtggaa caccggggct ttttgcatga
     4801 atatgaggga attttaagta tctaaaggag aacgcaacca tgaaactcaa gaatgctatg
     4861 gcacttggcc ttgcctccgt cctggccgtt ggtctgaccg cctgcggctc cagcgccgac
     4921 acctccgccg ccgaaagcac cgtggctgct gcggatgccg ccggtgcccc ggacggcgac
     4981 ggcgacccca ccaccctgac cgttgccatg gagtgcgcct acgcccccta caactggacc
     5041 cagagcgacg attccaacgg cgctgttgag attcgcggtt ccagcgacta cgcctacggc
     5101 tacgacgtta tgatggcgaa gaagatcggc gaggccctgg gccagaaggt tcagatcgtc
     5161 aagctggact gggacagcct gatccccgcc gtcatgagcg gcgacgttga ctgcgtcatc
     5221 gccggtcagt ccatcacggc tgagcgcgcc gcccaggttg acttcagcga cccctattac
     5281 tacgcttcca tcatcacgct gaccaagaag gacagcgcct acgccaacgc cgccagcgtg
     5341 gctgacctgg ccggtgcaac cgctaccagc cagctgggca ccatctggta tgacacctgt
     5401 ctgccgcaga tcgaaaacgc caacatcctg cccgcacagg agacggcccc cgccatgctg
     5461 gtggccctga acagcggtgc ctgcgacatc gtcgtcaccg accgccccac cggccaggca
     5521 gcactggtcg cctaccccga cttcacgctg ctggacttcg gcggcggcga cggtgacttc
     5581 caggtcagcg acgaggatat caacattggc atctcgatga agaagggcaa caccgcgctg
     5641 aaggatgcca tcaacgaggt gctggccacc atgaccaccg acgactacaa tgccttgatg
     5701 gatgaagcca tcagcgttca gcccctgagc gacaactaat accgtctgcc cctgccgttt
     5761 tgcgcggcag gggcttcggt gttgaggaaa cataaaggag actctcatgc aagacttttt
     5821 cgcaaactgt gccacagtgc tgcaaaagta cggcttctcg tacctgcgcg gtgcgggcac
     5881 taccctgctg ctggcactgg tcggcacgtt cttcggctgc ctaatcggct ttgcggtcgg
     5941 cgcattgcag accatcccgg tggataagca gcgcgacccg gtgtggaagc gcgtgctctt
     6001 gaaaattctg cacatcttcc tgcgctgcta tgtggagctg ttccgcggca cgcccatgat
     6061 cgtgcaggct gtgttcatct actatggcct tttgcaggtt tttggcatca aggtgggtat
     6121 gtggcaggcc ggcttcttca tcgtgtccat caacaccggc gcgtatatgg ccgagacagt
     6181 ccgcggcggt atcgtgtcca ttgacccggg ccagaccgag ggcgcaaaag ccatcggtat
     6241 gaaccactgg caaacgatga tccacgtcat tctgccccag gcactgcgca acatcatccc
     6301 gcagatcggc aacaacttca tcatcaacat caaggatacg tcggttctgt ccgttatctc
     6361 catcaccgac ctgttctttg tacacaaaag cgttgtcggc gcactgtaca cctactttga
     6421 gagcgcggcc atcgtcatgg tcatctacct gacgatgacg ctgtttgcca gctatctgct
     6481 gcgcctgtgg gagaaagcgc tggacggccc gcagaactac gacctgaaca ccaccgactc
     6541 gctggcctac accagcggta tgtacaacgc cccggacccg aaccacgagt cccagaatct
     6601 ggatatgaag ggaagtgcag cccatgtctg agcctgttat cgaggtgcgc cacctctcca
     6661 agagcttcgg cacccacgtt gtcctgcgtg acgttgattt taccgtcaac gcgggtgacg
     6721 tcacctgcat catcggtgcg tccggcagcg gcaaatccac actgctgcgc tgcatcaacc
     6781 tgttggagac gcccacgaac ggcaaagtgc tgttccacgg caacgatatc accgccaagg
     6841 ggtctgaccc ctgcgcctac cgcgccaagg tgggcatggt gttccagagc ttcaacctgt
     6901 tcaacaacat gactgtgctg gacaactgca cggc
//
LOCUS       DBMDSQ010000083         6870 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_2876_length_6870_cov_2.157630, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000083 DBMDSQ010000000
VERSION     DBMDSQ010000083.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 6870)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6870)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6870
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2876_length_6870_cov_2.157630"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            43..843
                     /gene="larE"
                     /locus_tag="ACZPAX_04400"
     CDS             43..843
                     /gene="larE"
                     /locus_tag="ACZPAX_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003443450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HIR1113171.1"
                     /translation="MDKLEALRQYLLELGSVAVAFSSGVDSTFLLKVAHDTLGDNAIA
                     VTARSCSFPKRELDEATAFCKKEGIRHFIVESEELQIEGFSHNPKNRCYLCKHELFEK
                     IIALAKENGVLYVAEGSNMDDNGDYRPGLQAVAELHVTSPLRRVGLTKQEIRNYSHQL
                     GLPTWDKQSFACLSSRFVYGEEITEEKLGMVDKAEQLLLDLGFHQLRVRIHGKMARIE
                     VLPAEFEKLLEHRGEIVTAFKKFGFTYVTMDLQGYRMGSMNETLGIKK"
     gene            903..1655
                     /gene="larB"
                     /locus_tag="ACZPAX_04405"
     CDS             903..1655
                     /gene="larB"
                     /locus_tag="ACZPAX_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004058152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HIR1113172.1"
                     /translation="MDTKHILEQVRDGAVSVEEAEQYFRRAPFEELGYAKLDTHRRLR
                     SGFAEVVFCSGKADDYLVGIFQRLAAEEGEVLGTRATPAQAALVQAACPGATYDPISR
                     LLKLEKHNKPLHGNIAVCTAGTADIAVAEEAAQTAEFFGSKVTRVYDVGVSGLHRLLA
                     NLESIQAANCVVAVAGMEGALASVIGGLVQNPVIAVPTSVGYGASFGGVSALLTMINS
                     CANGIATVNIDNGYGAGYLATQINRLALGAKE"
     gene            1659..3005
                     /gene="larC"
                     /locus_tag="ACZPAX_04410"
     CDS             1659..3005
                     /gene="larC"
                     /locus_tag="ACZPAX_04410"
                     /EC_number="4.99.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004056763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HIR1113173.1"
                     /translation="MDKRTLYLECNSGLSGDMFTAALLDLGADEAVLRKALASLPLDG
                     FDIKISRVKKSGLDACDFDVVLDAAHENHDHDMAYLYGDDHAHCHMHEHHHDHDHTHG
                     DAHDHAHGDHHHHHEHRGLAEILDILSRADLTPRARELAERIFTILGRAEAKAHGATL
                     ETVHFHEVGAVDSIVDITAAAVCLDNLGIEEVILPILYEGTGTIRCAHGVLPIPVPAV
                     VNIAAAEHLNLHITTAQGEYVTPTGAAIAAAIRTSETLPVAFTVEKIGLGAGKREQEL
                     PGFVRAMLIHPHSAADGDAQDTICKLETNIDDCTGEALGYVMERLMAAGARDVYYTPV
                     YMKKNRPAYLLAVLCLPEDAAAMEQIIFSETTTIGIRRVKMERCVLKRRLRTVQTSLG
                     SVQVKECEVPAKGDAVHIRCYPEYESVRQLCREQGCNYQDACRTILKELDAKEMEN"
     gene            3009..4025
                     /locus_tag="ACZPAX_04415"
     CDS             3009..4025
                     /locus_tag="ACZPAX_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015261140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HIR1113174.1"
                     /translation="MHMADALLSPGVAGVMYAASAVAAGHSVHKLEQNRDTTDKIIPT
                     MGVMGAFVFAAQMINFTIPGTGSSGHLCGGMLLTAIVGPYAAFLTMIGVLLIQCLLFA
                     DGGLMALGANVWNMAFYGCFVGGLLWKAMMQRGISKRKIVVASLLGCVLTLQMGALSV
                     SVETLASGITELPFTAFLLAMQPIHLAIGLVEGAITAAVLVFLQQARPALLWQTQTID
                     EAKQPLTLRRTLGIVAVAAAMTAGVLSLFASAFPDGLEWSLQRITGSTELDASGPVQA
                     FFARIQNLTALLPDYNLTGSESAAGASAAGLIGAAIVLAAVVVLGKILKKAAQRHGSS
                     ASRD"
     gene            4000..4806
                     /gene="cbiQ"
                     /locus_tag="ACZPAX_04420"
     CDS             4000..4806
                     /gene="cbiQ"
                     /locus_tag="ACZPAX_04420"
                     /inference="COORDINATES: protein motif:HMM:NF014420.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0032778 - P-type cobalt transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HIR1113175.1"
                     /translation="MDRLHRAIKNVHRLDDLAARGTALTRLPPLPKLLAVLVYLCVTL
                     SFQKYALTGLLGMALWPILLYEIDDLSIVKTLRQCWAVVALLVLVGIANLFFDRAPVL
                     TLGPVVLSGGAVTLLVLVVKGVLCFLAVYLLIATTGMERVCAALRQLHIPRVLVTTIL
                     LTYRYIVLLLQEGNRISTAYALRAPGEKGIHFRAWGSLLGQLLLRSVDRAQLVYESMM
                     LRGYGWRDDPAPTRQPQKGSAAGTVFFVATLMYCILFRVIPIFELLGQLF"
     gene            4819..5562
                     /locus_tag="ACZPAX_04425"
     CDS             4819..5562
                     /locus_tag="ACZPAX_04425"
                     /EC_number="3.6.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005358986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HIR1113176.1"
                     /translation="MTPILELQDLSFRYEGGLPGEKVLDALTFAVAPGERVGLVGCNG
                     VGKSTLLKILVGILPIQTGALTVGGLPMERKNLAEIRRKVGYIFQDSDSQLFMPTVRA
                     DVAFAAENYGYPPEEVAARTRRALTAVHMEHCADRPVYRLSGGQKKLASIAGILTLDP
                     ALILMDEPSAALDPRNRRALIGILNALPCAKLIASHDLDFIYDTCDRVLLLHHGQLAA
                     DGPAREILCDKTLLEKCELELPLRWQRDF"
     gene            5596..6477
                     /locus_tag="ACZPAX_04430"
     CDS             5596..6477
                     /locus_tag="ACZPAX_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008117042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HIR1113177.1"
                     /translation="MSKSTVYFTNELTPEAVVKMYKQLGVELPGKVAVKVHSGEDGNQ
                     NYLHPEFMKPVIDAVHGTVVECNTAYGGARNETPKHIELMKKHRWSDLFDVDIMDAEG
                     PDVEWPVENGKVLKVNYLGKNIENYDSMLVLIHFKGHPMGGYGGALKQLSIGCASSAG
                     KTYIHSGGKVTDQNVLWENCAPQDTFLEAMADAASTVAKHFAGKIAYVAVMKNLSVDC
                     DCCAVAEDPCMDDVGILSSLDPVALDRACVDLVMNSNDPGRDHFMERVNSRHGTHTID
                     TAAELGIGSKEYELVRV"
     gene            6627..>6870
                     /locus_tag="ACZPAX_04435"
     CDS             6627..>6870
                     /locus_tag="ACZPAX_04435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HIR1113178.1"
                     /translation="MVHVPALAGVAGSRVIDNAGQRFLTMLLVWSIPCGTTLSLAPML
                     GGIFFGPVGSALVLVLMAAMTIGSMYLARCIFGKQLV"
BASE COUNT         1354 a         2028 c         2134 g         1354 t
ORIGIN      
        1 cttggtgtat agaaaatcgt cgaaaagcga ggctgaacat agatggacaa attggaagca
       61 ttgcggcagt atctgctgga gctgggcagc gtggcggtgg ccttttccag cggtgtggat
      121 tccacattct tattaaaggt agcgcacgac acgctgggcg acaatgctat tgccgtgacc
      181 gcgcggagct gttctttccc caagcgggag ttggacgagg caacggcgtt ctgcaaaaag
      241 gaaggaatcc gtcactttat tgtggagtct gaggaactgc aaatcgaggg tttctcccac
      301 aatcccaaga accgctgcta cctgtgcaag catgaattgt tcgaaaaaat catcgcgtta
      361 gcgaaggaga acggcgttct gtatgtggca gagggttcca acatggacga caacggcgac
      421 taccgccccg gcttgcaggc cgtagccgag ctgcacgtga ccagtccctt gcgccgtgtc
      481 ggcctgacga agcaagagat tcgcaactac tcccaccagc tggggctacc cacctgggac
      541 aaacagtcct ttgcctgcct gtcgtcgcgg ttcgtctacg gcgaggaaat taccgaggaa
      601 aaattgggca tggtggacaa ggctgagcag ctgcttttgg atttagggtt ccaccaactg
      661 cgggtgcgca tccacggcaa aatggcgcgt atcgaggtgc tgcccgcaga gtttgagaag
      721 ctgctggaac accggggaga aatcgttaca gcgtttaaaa agtttggttt tacctatgtg
      781 acgatggatc tgcagggata taggatgggg agcatgaacg agacattggg gattaaaaag
      841 taagggagcg tccgaaaagc caatccccta ccctgctctc caatctcacg aaaggacaaa
      901 ccatggacac caaacacatt ctggaacagg ttcgcgacgg cgcggtgagc gtggaggagg
      961 cggaacaata cttccgccgc gcgccgtttg aggaactggg ctacgccaag ctcgacaccc
     1021 accggcggct gcggtccggc tttgccgagg tcgttttttg cagcggcaaa gctgacgact
     1081 acctcgttgg tatcttccaa cggctggctg ctgaggaagg cgaagtgctg ggcacccgcg
     1141 ccacacccgc ccaggccgcg ctggtgcagg ccgcctgccc cggcgcgacc tatgacccca
     1201 tcagccgcct gttgaaactg gaaaagcaca ataaaccgtt acacggcaat attgccgtct
     1261 gcaccgcagg cacggcggac attgccgtgg ccgaggaagc cgcccagacc gccgagttct
     1321 tcggcagcaa agtcacccgc gtctacgacg tcggcgtcag cgggctgcac cgcctgctgg
     1381 cgaacctgga gtcaatccag gccgccaact gcgttgtggc cgtggcgggc atggagggcg
     1441 cacttgccag cgtcattggt gggctggtgc aaaacccggt cattgcggtg cccacctcgg
     1501 tggggtacgg cgcgtccttc ggcggcgtgt cggccctgct gacgatgatc aactcctgcg
     1561 ccaacggcat tgccaccgtc aacattgata acggctacgg cgcgggctac ttggcaaccc
     1621 agatcaaccg cctggcactg ggcgcgaagg agtagaaaat ggacaagcga acgttatatt
     1681 tagagtgtaa ctctggcctg agcggtgata tgttcaccgc cgccctgctg gacttgggcg
     1741 cggacgaagc tgtgctgcgc aaggcgctgg ccagcctgcc gctggacgga tttgacatca
     1801 aaatatctcg cgtcaaaaaa tccgggctgg acgcctgtga ttttgacgtg gtgttggacg
     1861 ccgcccacga gaaccacgac cacgacatgg cgtacctcta cggcgacgac cacgcacatt
     1921 gccatatgca tgaacatcac catgaccacg accataccca tggcgacgcg catgaccatg
     1981 cccacggcga ccaccatcat catcacgagc accgcgggtt ggcggaaatc ctcgatatcc
     2041 tctcccgtgc agatttgacg ccccgggcgc gggagctggc cgagcgcatc ttcacgattt
     2101 tgggcagggc cgaagcaaaa gcccacggtg caacgctaga aaccgtgcat ttccacgagg
     2161 tcggcgcggt ggattccatc gtggatatca ccgccgctgc tgtttgcctg gacaacctgg
     2221 gcatcgagga agtcatcctg ccgatcctct atgagggcac cggcaccatc cgctgcgccc
     2281 acggcgtgct gcccatcccg gtgccggccg tcgtcaacat tgcggcagcc gagcacttga
     2341 acctgcacat caccaccgcc cagggcgagt acgtgacccc caccggcgcg gccattgcgg
     2401 cggccatccg caccagcgag acgctgcccg tcgcgttcac cgtggaaaag atcggcctgg
     2461 gcgcaggcaa gcgcgaacag gagctgcccg gctttgtgcg ggccatgctc atccaccccc
     2521 acagcgctgc ggacggcgac gcgcaggaca ccatctgcaa gctggaaacc aacatcgacg
     2581 actgcaccgg cgaggccctg ggttacgtga tggagcggct gatggccgca ggggctcggg
     2641 acgtgtacta cacgccggtc tacatgaaaa agaaccgccc cgcgtatctt ttggctgtgc
     2701 tctgcctgcc ggaggacgcc gccgccatgg agcaaattat tttttccgag acgaccacca
     2761 tcggcatccg ccgcgtaaaa atggagcgct gcgtgctgaa acgccgtctg cgcaccgtgc
     2821 agacgagcct gggcagcgtg caggtcaagg agtgcgaggt ccccgccaag ggcgacgccg
     2881 tgcacatccg ctgctacccc gagtacgaga gcgtgcgcca gctctgccgc gagcagggct
     2941 gtaactacca ggatgcctgc cgaacgattt taaaagaact tgatgcaaaa gaaatggaga
     3001 attgaattat gcacatggca gatgcgttgc tttcccccgg tgtggccggg gtgatgtatg
     3061 cggcctcggc ggtcgcagcc ggacacagcg tacacaaact ggagcaaaac cgcgacacaa
     3121 cggataaaat cattcccacc atgggcgtca tgggcgcgtt cgtttttgcg gcgcagatga
     3181 taaacttcac gattcccggc accgggtcgt cgggccacct ctgcggcggt atgctgctga
     3241 cggccattgt ggggccgtat gcggcgtttt tgacgatgat cggcgtgcta ctcatccaat
     3301 gcctgctgtt cgccgacggc ggcctgatgg cgctgggcgc gaacgtctgg aacatggcgt
     3361 tctacggctg ctttgtgggc ggtctgctct ggaaagcgat gatgcagcgc ggaatttcta
     3421 agcgcaaaat cgtcgttgcg tcgctcttag gctgcgtgct gaccctgcaa atgggcgcgc
     3481 tgtccgtctc ggtggagacg ctggcaagtg gcatcaccga gctgcccttc accgcgtttt
     3541 tgctggcaat gcagcccatt cacctggcga ttggcctggt ggaaggggcc atcacagcgg
     3601 cggtgctggt gtttttgcag caggcccggc ccgcacttct gtggcagacc cagactatcg
     3661 acgaggccaa gcagccgctg acgctgcgcc gcacgctggg cattgtggcc gtggcggcgg
     3721 cgatgacggc gggggtgctg tcgctgttcg cgtcggcctt cccagatggg ctggaatggt
     3781 cgctgcagcg cataaccggc agcactgaac tggacgccag tggcccggtg caggcgtttt
     3841 ttgcccgcat ccaaaacctg accgcgctgc tgcctgacta caatttaacc gggtcggaaa
     3901 gcgccgcagg ggcctcggcc gccggtttga ttggcgctgc catcgtgctg gcggcggtgg
     3961 tcgtgctggg caaaattctg aaaaaggcgg cacagcgaca tggatcgtct gcatcgcgcg
     4021 attaaaaatg tccaccggct ggacgatttg gccgcccgcg gcacggctct gacccggctg
     4081 ccgccgctgc ccaaactgct ggcggtttta gtctatctgt gtgtgaccct atcgttccaa
     4141 aaatacgcct tgactgggct gctgggcatg gcgctgtggc cgatactgct gtatgaaatc
     4201 gacgacctat cgattgttaa aacgctgcgg cagtgttggg ctgtcgtggc gctgctggtg
     4261 ctggtgggca tcgccaacct gttttttgac cgtgcaccgg tgctgaccct ggggccggtc
     4321 gtgctgtccg gcggggcggt cacgctgctg gtgctggtgg tcaagggggt gctctgcttc
     4381 ctggcggtct acctgctgat tgccaccacc ggcatggagc gggtctgtgc cgcgctgcgc
     4441 cagctgcata tcccccgggt gctggtgacg acaattttgt tgacctatcg ttatattgtg
     4501 ctgcttctgc aggaggggaa ccgaatctcg acggcctacg ccctgcgtgc accgggggag
     4561 aagggcatcc atttccgcgc ctggggttca ctgctggggc agctgctatt gcggagtgtg
     4621 gatcgggcgc agctggtgta tgagagtatg atgttaaggg ggtatgggtg gcgcgacgac
     4681 cccgccccta cccggcaacc gcagaagggc agcgccgcag gcacggtttt ctttgttgca
     4741 acgcttatgt attgcatcct cttccgcgtc atccccattt ttgaacttct cggccaactt
     4801 ttttgaaagg agagcatcat gacaccaatt ttagaattgc aagacctctc cttccggtat
     4861 gagggcggac tgcccgggga gaaagtgctg gacgcgctga cctttgccgt cgcgccgggc
     4921 gagcgcgttg ggctggtggg ctgcaacggt gtgggcaaat ccacgctgct gaaaatcctt
     4981 gtgggcatcc tgccgataca gacgggggca ctgaccgtgg gcggtctgcc gatggaacgc
     5041 aaaaatctgg cggaaatacg ccgaaaagtc gggtacatct tccaggattc cgacagccag
     5101 ctttttatgc ccaccgtgcg ggccgatgta gcctttgccg ccgagaatta cggctatccg
     5161 cccgaggaag tcgccgcccg cacccggcgt gcgctgaccg ccgtccacat ggagcactgc
     5221 gccgatcggc cggtataccg gctttccggc ggtcaaaaga agctcgcgtc catcgcgggc
     5281 atcctaacgc tggatcccgc gctgatcctg atggacgagc cgtccgcggc gctggacccc
     5341 cgcaaccggc gggcgctcat cggcattttg aacgcgctgc cctgcgcgaa gctgattgcg
     5401 tcccacgacc tggatttcat ctacgacacc tgcgaccggg tgctgctttt gcaccacggc
     5461 cagctcgccg ccgacggccc cgcgcgggag attttgtgcg ataaaacgtt gttagaaaag
     5521 tgcgaattgg agttgccgtt gaggtggcag cgggattttt aatcacgttc ctatttttaa
     5581 aagggggacc ttatcatgtc taaatccacc gtttacttta ccaacgagct cacgcccgag
     5641 gcggtcgtga aaatgtataa gcagctgggc gtggaactgc ccggcaaagt cgccgtcaag
     5701 gtccacagcg gcgaggacgg caaccagaac tacctgcacc ctgagttcat gaagccggtc
     5761 atcgacgccg tgcatggcac cgtggtcgag tgcaacaccg cttacggcgg tgctcgcaac
     5821 gaaaccccca agcacatcga gctgatgaaa aagcaccgct ggagcgacct ttttgacgtg
     5881 gacatcatgg acgccgaagg ccccgacgtg gagtggccgg tcgagaacgg caaggtgctg
     5941 aaggtcaact acctgggcaa gaacattgaa aactacgaca gtatgttggt tcttatccac
     6001 tttaaggggc accccatggg cggctacggc ggtgcgctga agcagctgtc catcggctgc
     6061 gcgtccagcg cgggtaagac ctacatccac tccggcggca aggtcaccga ccagaacgtg
     6121 ctgtgggaga actgcgcccc gcaggatacc ttcctggagg ctatggccga cgccgcttcc
     6181 accgtagcca agcactttgc gggcaagatt gcctatgtgg ccgtgatgaa gaacctgtcc
     6241 gttgactgcg actgctgcgc cgtggccgag gacccctgca tggacgatgt gggcattttg
     6301 tccagcctgg atcccgttgc gctggaccgt gcctgcgtgg acctggtcat gaacagcaac
     6361 gaccccggcc gcgaccactt tatggagcgc gtcaacagcc gccacggcac ccacaccatc
     6421 gataccgccg ccgagctggg cattggcagc aaagagtacg agctggtgcg cgtgtaaccc
     6481 ccctgccgca taaaacaatg catcccccga cgcaatgcgt cgggggattt ttgcgtttat
     6541 gtttcgtttg tctgcaatcg ggtctggcga cggcaatcca acgattttac gtgatatttc
     6601 tcgtagaata ccctagcaaa acggccttgg ttcacgtacc cgcactggcg ggcgtcgcgg
     6661 gcagccgcgt cattgacaac gccgggcagc gctttttgac gatgctgctg gtgtggagca
     6721 tcccctgcgg cacgacgctg tcccttgcgc cgatgctggg cggcatcttc ttcggcccgg
     6781 tgggcagtgc gctggtgttg gtgctgatgg ccgccatgac catcggctcc atgtatctgg
     6841 caaggtgcat ctttggcaaa cagctagttg
//
LOCUS       DBMDSQ010000084         6697 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_2943_length_6697_cov_1.804730, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000084 DBMDSQ010000000
VERSION     DBMDSQ010000084.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 6697)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6697)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6697
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2943_length_6697_cov_1.804730"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            47..400
                     /locus_tag="ACZPAX_04440"
     CDS             47..400
                     /locus_tag="ACZPAX_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007045499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR1113179.1"
                     /translation="MAFAAWWGILNLVLTVALLAAVVYLFVLIVRALKKYIGSKEVRA
                     EKRAAKRTLGETLKAHRIQCHMTQEFVAESLGVSRQTVSKWENGSADPSTTNLLAVAK
                     LYDVPAEELLREVER"
     gene            complement(500..1261)
                     /locus_tag="ACZPAX_04445"
     CDS             complement(500..1261)
                     /locus_tag="ACZPAX_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007045991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HIR1113180.1"
                     /translation="MAEKTGVQSVERIFQLIEQLAAHPTGVSLQKLAGETGLAKSTVH
                     RLLASLVGLGYVAQNDENGHYRLTLKMFELSSGIVDSMDIMGVAKAHLERLSQRTGEA
                     VHLVIRDGRDIVYIYKTESGPMRMSSRVGLRSPLYCTGVGKAILATLPGDELEDIWTH
                     SNVQKLTDRTITDLEELRSQLVEVRANGYAIDDEENEVGVRCVAVAIPGADGRAESAF
                     SISGLAPYMTPERIRRIATLALDARTDILADLGLR"
     gene            1557..2519
                     /gene="eda"
                     /locus_tag="ACZPAX_04450"
     CDS             1557..2519
                     /gene="eda"
                     /locus_tag="ACZPAX_04450"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007045990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008675 -
                     2-dehydro-3-deoxy-phosphogluconate aldolase activity
                     [Evidence IEA];
                     GO_function: GO:0008700 - (R,S)-4-hydroxy-2-oxoglutarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HIR1113181.1"
                     /translation="MNSVLQRVYEIGIIPVIAFNSADEALPLCKALMDGGLPAAEVTF
                     RTACAEECIKKIHEELPDMLLGAGTVLTIDQADRAMAAGASFIVAPGFDPEVCQHVID
                     KGGIMMPGTATAGEMQQAMNMGCECVKFFPAEANGGVDKLKNIGAALKTCKWMCTGGV
                     NAKNVNNYLGYNQIIAVGGTWMCKSDKIKAGAWDEIAAMSREAVDVMLGLELGHIGIN
                     CENEAEAAKTAETIASLLSMAVKPGNSSIFVGKKEFEIMKKPGRGTNGHIAILTNNVD
                     RAIYHLGQRGVKFDMDSKNVKDGKTVAIYFADEIAGFAFHLVQR"
     gene            2612..3637
                     /locus_tag="ACZPAX_04455"
     CDS             2612..3637
                     /locus_tag="ACZPAX_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015565280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HIR1113182.1"
                     /translation="MKVVTFGELMIRLQPFNYERFVQANNLEFTFGGGEANVAVSLAN
                     YGIDAAFVTKLPAHAIGQAAVNSLRRYGVDTSKITRGGDRVGIYFNEKGASQRGSVCI
                     YDRANSAIQLASTADFDWDAIFEGVDWFHFTGITPALGENVVEICREACKAAKAHGVK
                     ISCDLNYRGKLWTREQARAAMTDLCQYVDVCISNEEDAKDVFGIEAEATDIYAGEINR
                     EGYKSVAKQLADKFGFEKVAITLRESHSASDNGWSAMLYDVASNEYCFSKKYELRIID
                     RVGGGDSFGGGLIYALLNGKSTQDAVEFAVAASALKHSIEGDYNMVTVSEVEKLAGGD
                     GSGRIQR"
     gene            3839..4819
                     /locus_tag="ACZPAX_04460"
     CDS             3839..4819
                     /locus_tag="ACZPAX_04460"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin--[biotin carboxyl-carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HIR1113183.1"
                     /translation="MVKQEVLAALDGARGQYISGQELAARLGVSRTAIWKAVAALRSD
                     GIPIEAVTNRGYTLSKNVDVLNTAAVAALLHEAPLQALQIEVVDRLPGTNSVLRARAD
                     NGAPEGLVLIAQAQSAGRGRGGHSFYSPPGGLYLSVLLRPEIGARQAVGLTAMAAVAA
                     ARAAEKLCGTPIRIKWVNDLWKNGKKVCGILTEAALNLESGMLDYAVLGLGFNVIAPA
                     EGWPDDLRDVAGALYDGTPAPGARAMLAAAFLNEFWPLYRAGPRSGCLDEYRSRQALV
                     GRQVLVTPRQGEPRQAAALGINDEWKLIVRFTGESRPTALNSGEVSVRLL"
     gene            4841..5386
                     /locus_tag="ACZPAX_04465"
     CDS             4841..5386
                     /locus_tag="ACZPAX_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007045987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HIR1113184.1"
                     /translation="MTKTSNNVRRMVLCAMFTALVAVCSQIAVPMPWGVPINLALFAV
                     YMAGTMLGPVWGTASQVVFIALAAIGVPVLANFQGGPSAIFGKTGGYAIGYILTALIT
                     ALLSKVLGRKFWALCVAMVVGCAACYVLGTIWFMILTGADLVSALGWCVLPYLPGDAV
                     KIALASALTIQLDKRLKGSLV"
     gene            5694..6152
                     /gene="ruvX"
                     /locus_tag="ACZPAX_04470"
     CDS             5694..6152
                     /gene="ruvX"
                     /locus_tag="ACZPAX_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007045986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HIR1113185.1"
                     /translation="MKWLAVDYGDSRTGLAGCDAGETITSPITPQIEEKSMNKVAAAV
                     TEVLTARGAEGLVCGLPKNMDGTEGSRANKSRRFAQRLANTAGVPVVMWDERRTTVSA
                     AAILADNDTFGAKRKERLDSVSAAVILESFLNWRAHHPGEEPPELVRPEQ"
     gene            6166..>6697
                     /gene="uppS"
                     /locus_tag="ACZPAX_04475"
     CDS             6166..>6697
                     /gene="uppS"
                     /locus_tag="ACZPAX_04475"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HIR1113186.1"
                     /translation="MPNAAEPRFPTHVAIIVDGNRRWAKQRLMPVSMGHKAGFDRLRE
                     ITRYAVGDRGVYVLSLYVFSTENFSRDAKEVGYLMDLFANNFRTIADEMNERGCKVLF
                     SGRREGLQQRVLDAMDYMMNLTKDNDKGIVNFCLNYGSHAELTDATRAIAAEVKAVIA
                     EVGATSMKEMGKVMGVA"
BASE COUNT         1319 a         2041 c         2038 g         1299 t
ORIGIN      
        1 ggctggtata ctgggggcag aaaaatccac gaaggaggaa atagagatgg catttgcagc
       61 atggtggggg attttgaatc ttgtgctgac ggtagcgctg ctggcggcgg tcgtgtacct
      121 gtttgtgctg atcgtgcggg cgctgaaaaa gtacatcggc agtaaagaag tccgggcgga
      181 aaagcgggcc gccaaacgca ccctgggtga gacgctgaaa gcccatcgaa tccaatgcca
      241 catgacgcag gagtttgtgg ccgagagttt aggcgtcagc cgccagacgg tctccaagtg
      301 ggagaacggc agcgcagacc ccagcacaac aaatttgctg gctgtggcaa agttatacga
      361 tgtgccagca gaggagcttc tgcgggaagt agaaaggtag gggggcgtcc tcgacgcccc
      421 cctacgacta aaggataaag ttgtactaaa ggcaagatgc ggcggccagg gggctggccg
      481 ccctacaact ctatatcttt taccgcagcc ccagatccgc cagaatatcc gtccgtgcgt
      541 ccagcgccag cgtcgcaatt ctgcgtatcc gctcgggtgt catatagggc gctaacccgc
      601 tgatggaaaa cgcactttcc gcgcggccgt cggcgccggg gatggccacc gccacgcatc
      661 gcacaccgac ttcgttctcc tcgtcgtcga tggcgtagcc gttggcgcgc acttccacaa
      721 gctggctgcg cagctcctcc aaatcggtga tggtccggtc ggtcagcttc tgcacgttgc
      781 tgtgggtcca gatatcctcc agctcatccc ctggcagcgt ggctaaaata gccttgccca
      841 cgccagtgca gtacagcggg ctgcgcaggc ccacgcggct ggacatccgc atgggaccgc
      901 tctcggtctt ataaatgtag acgatgtccc gcccatcgcg gatgaccagg tgcacggcct
      961 cgccggtgcg ctgggagagg cgctccaaat gcgcctttgc cacgcccata atatccatgc
     1021 tgtccacaat gccgctggac agctcaaaca tttttaaggt cagccggtag tggccattct
     1081 cgtcgttctg cgccacatag cccagcccca ccaggcttgc cagcaggcgg tgcacggtgc
     1141 ttttggcaag gccggtctcc cctgccagct tttgcaggct gacccccgtg gggtgcgccg
     1201 ccagctgctc gatcagctga aagatgcgct ccacactctg cacgccggtc ttttctgcca
     1261 tgataagggc ctcctgacga aatttaacaa aaatttcata aatttaattt gcgggacgga
     1321 acgcataatc gttccgtatt atggaacgat tataacagag ttggccgatt ttttcaagct
     1381 cttttccgaa ttgcggaatc cttggcagat acaacaaaaa tcgtgcgctt tctttcacat
     1441 ttctgcccga ttgacgccca ccgacccagt ggatataata aaagtaacca agtgcgtgac
     1501 cccgccgcag tgtgtgaact gggtggcccg cacagaatca gtgaaggaga attgctatga
     1561 attctgtttt gcagcgtgtt tacgagatcg gcattatccc ggtcattgct ttcaacagcg
     1621 cggacgaggc cctgcccctg tgcaaggccc tgatggacgg cggcctgccc gccgccgagg
     1681 tcaccttccg caccgcttgt gctgaggagt gcatcaagaa gatccacgag gaactgcccg
     1741 atatgctgct gggcgctggc actgtcctga ccattgatca ggctgaccgc gctatggccg
     1801 ccggcgcttc cttcatcgtt gcccccggct ttgaccccga ggtctgccag cacgtcatcg
     1861 acaagggcgg catcatgatg cccggcaccg ccactgcagg cgagatgcag caggccatga
     1921 acatgggctg cgagtgcgtt aagttcttcc ccgcagaggc caatggcggc gtggacaagc
     1981 tgaagaacat cggcgctgcc ctcaagacct gcaagtggat gtgcaccggc ggcgtcaacg
     2041 ccaagaatgt caacaactac ctgggctaca accagatcat cgccgtcggc ggcacctgga
     2101 tgtgcaagag cgacaagatc aaggccggtg cttgggacga gatcgccgca atgagccgcg
     2161 aggccgttga tgtcatgctg ggccttgagc tgggccacat cggcatcaac tgcgagaacg
     2221 aggccgaggc cgccaagacc gctgagacca tcgccagcct gctgagcatg gctgtcaagc
     2281 cgggcaactc cagcatcttc gtgggcaaga aggaattcga aatcatgaag aagccgggcc
     2341 gcggcaccaa tggccacatc gcgatcctga ccaacaatgt tgaccgtgct atctatcacc
     2401 tgggccagcg cggcgtcaag ttcgacatgg acagcaagaa tgtcaaggac ggcaagaccg
     2461 ttgccatcta ctttgctgac gagattgcag gctttgcctt ccacctggtc cagcgctgat
     2521 ttggcgtaat cccaatgtaa atccaggggg tggcgtcctc gacgccccac tcccctatct
     2581 ttaaaaaata ttgaattaaa aggagttcac catgaaagtc gttacttttg gcgaattgat
     2641 gatccgtctg cagccgttca actacgagcg ctttgtccag gccaacaacc tggagttcac
     2701 cttcggcggc ggcgaggcca acgttgctgt ttctctggcc aactacggca ttgacgctgc
     2761 tttcgttacc aagctgcccg cccatgccat cggtcaggcc gctgtgaaca gcctgcgccg
     2821 ctacggcgtt gacaccagca agatcacccg cggcggcgat cgcgttggta tctacttcaa
     2881 cgagaagggc gcttctcagc gtggttccgt ctgcatctac gaccgcgcca actccgccat
     2941 tcagctggct tctaccgccg acttcgactg ggatgccatc ttcgagggcg tggactggtt
     3001 ccacttcacc ggcatcaccc cggccctggg cgagaatgtt gttgagatct gccgcgaggc
     3061 ctgcaaggct gctaaggctc atggcgtcaa gatcagctgc gacctgaact accgcggcaa
     3121 gctgtggacc cgtgagcagg cccgcgctgc catgactgac ctgtgccagt acgttgacgt
     3181 ctgcatctcc aacgaggagg atgcgaagga cgtctttggc attgaggctg aggctaccga
     3241 catttacgcc ggtgagatca accgcgaggg ctacaagagc gttgccaagc agctggctga
     3301 caagttcggc ttcgagaagg tcgccatcac cctgcgtgag agccattccg cttccgacaa
     3361 cggctggagc gctatgctgt acgatgtcgc ttccaacgag tactgcttct ccaagaagta
     3421 cgagctgcgc atcatcgacc gcgttggcgg cggtgactcc ttcggcggcg gcctgatcta
     3481 tgccctgctg aacggcaaga gcacccagga tgctgttgag ttcgctgttg ctgcttctgc
     3541 cctgaagcac tccatcgagg gtgactacaa catggtcact gtctccgagg tcgagaagct
     3601 ggccggtggc gacggttccg gccgtatcca gcgctaagtt tagacttttc ccactaaatc
     3661 tgtttgcatc atagcaaaag ccccgcaggc gagagcctgc ggggcttttg ccgttcccgc
     3721 ctccccggcg ggacattttt tgctctgatt gtcaaccatg acgccgggaa ggcgttgaca
     3781 ttccggcccc cgccgcggta tactggctta cagtcgggcc tgaaaggggg gcggcagtat
     3841 ggtcaaacag gaagttctcg cagcgctgga cggcgcacgg ggacagtata tctccggtca
     3901 ggagctggcc gctcggctgg gcgtgagccg caccgccatc tggaaggctg tggctgccct
     3961 gcgcagcgac ggcattccta tcgaggccgt gacgaaccgc ggctacaccc tgtcgaaaaa
     4021 tgtcgatgta ctgaacactg ccgccgtagc ggccctgctg cacgaagcac ccctgcaggc
     4081 gctgcaaatc gaggtcgttg accgcttacc cggcaccaac agcgtactgc gtgcccgtgc
     4141 cgacaacggt gcccccgagg ggctggtgct catcgcccag gcgcagagcg cggggcgcgg
     4201 acgcggcggg catagctttt attcgccgcc cggggggctg tatttaagcg tgctgctgcg
     4261 gcccgagatc ggtgcccggc aggccgtggg cctgaccgcc atggcggcgg tggcagcggc
     4321 ccgcgcagcg gaaaagctct gcggcacgcc catccggatc aaatgggtca acgacctgtg
     4381 gaagaacggc aaaaaggtct gcggcatcct gaccgaggcc gcgctgaatc tggaatccgg
     4441 gatgctggac tacgccgtgc tggggctggg cttcaacgtc attgccccgg ccgagggctg
     4501 gcccgacgac ctgcgggacg tggcgggggc actgtatgat ggcaccccgg cccccggggc
     4561 gcgggctatg ctggcggcgg cattcttgaa cgagttctgg ccgctctacc gcgccgggcc
     4621 gcgcagcggc tgcctggacg agtaccgcag ccgccaggcc cttgtagggc ggcaggtgct
     4681 cgtcacgccg cgccagggcg agccgcgtca ggccgcagcg ctgggcatca atgacgaatg
     4741 gaaactgatc gtgcgcttta ccggcgaaag ccgccccacc gccctgaaca gcggcgaggt
     4801 cagcgtacgt ctgttataaa tgtaagaggg gtattttatc atgacaaaaa cttccaacaa
     4861 tgtccgccgc atggtgctat gtgccatgtt caccgcgctg gtcgccgtgt gcagccagat
     4921 tgccgtgccg atgccgtggg gggtgcccat caacctggcg ctgttcgccg tttatatggc
     4981 gggcacgatg cttggccccg tgtggggcac ggccagtcag gtcgtgttca ttgcgctggc
     5041 agccatcggt gtgccggtgc tggccaactt ccagggcggc ccgtctgcca tcttcggcaa
     5101 gaccggcggc tatgccatcg gctacattct gacagcgctc atcacggccc tgctgtccaa
     5161 ggtgctgggc cgcaagttct gggcgctctg cgtcgccatg gtcgtgggct gcgctgcctg
     5221 ctacgtgctg ggcacgatct ggttcatgat cctgacgggg gctgatttag tcagcgcttt
     5281 gggctggtgc gtcctgccct acctgcccgg cgacgccgtg aaaatcgccc tcgcctccgc
     5341 tttgaccatc cagctggata agcggctgaa aggctcgctg gtctgagttc gtaggggggc
     5401 ggcgtcctcg acgccccgcg ggtcgatgca agcatcgacc cctacagtac cccaagggct
     5461 tcccccacgg gggaagctgt caccgaaggt gactgatgag ggcagagctg atcaggctga
     5521 cccgtttacg ggcaaccgta gcaagcccgc ccctcatccg gccctgcggg gccaccttcc
     5581 cccctcgggg gaaggcaaag caacaaggca aacaaaatct cccgtcctgc tggtcagggc
     5641 ggttttttta tgctataata accctatacg acgaaatgta aaggagcctg cccatgaaat
     5701 ggcttgccgt tgactatggc gattcccgca ccgggcttgc cgggtgcgac gccggggaga
     5761 cgatcacctc ccctatcacg ccgcaaatcg aagaaaaaag catgaacaag gtcgctgccg
     5821 ctgtgactga ggtgctgacc gctcgcgggg ccgagggcct tgtctgcggt ctgcccaaaa
     5881 acatggacgg caccgagggc agccgcgcca acaagagccg ccgctttgcc cagcgccttg
     5941 ccaacaccgc cggtgtgccg gttgtgatgt gggatgagcg ccgcaccacc gtgtccgccg
     6001 ccgccatcct ggccgataac gataccttcg gcgccaagcg taaggaacgg ctggattccg
     6061 tgtccgccgc cgtcattttg gagagctttc tgaactggcg cgcccaccac cccggcgagg
     6121 aaccgcccga gctggtgcgc cccgagcaat aaaaggagaa cgattatgcc gaatgcagct
     6181 gaaccccgct tccccaccca tgtggctatc attgtggacg gcaaccgccg ctgggccaaa
     6241 cagcgcctga tgccggtcag catgggccac aaggctggtt ttgaccgcct gcgcgagatc
     6301 acccgctacg ccgttggcga ccgcggtgtc tatgtgctgt ccctctatgt tttctccacg
     6361 gagaatttca gccgcgacgc aaaggaagtc ggttatctga tggacctgtt tgccaacaac
     6421 ttccgcacca ttgccgacga aatgaacgag cgcggctgta aggtgctgtt cagtggccgc
     6481 cgcgaggggc tgcagcagcg cgtgctggat gccatggact acatgatgaa cctgacgaaa
     6541 gacaatgaca agggtatcgt caacttctgc ctgaactacg gcagccatgc cgagctgacc
     6601 gacgccaccc gcgccattgc cgccgaggtc aaggccgtga tcgccgaggt cggcgcgacg
     6661 tcgatgaagg agatgggcaa ggtgatgggc gtcgctt
//
LOCUS       DBMDSQ010000085         6681 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_2954_length_6681_cov_1.441033, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000085 DBMDSQ010000000
VERSION     DBMDSQ010000085.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 6681)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6681)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6681
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2954_length_6681_cov_1.441033"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1092
                     /gene="tilS"
                     /locus_tag="ACZPAX_04480"
     CDS             <1..1092
                     /gene="tilS"
                     /locus_tag="ACZPAX_04480"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HIR1113187.1"
                     /translation="GIEIPPHPSEDWARGLRYGWFDELAAREEAVIATAHTMSDQAET
                     VLFRMARGTGLHGLAGIPPRRGFYVRPCLCLTRADTEAYCAALGQHYIQDETNAEDTY
                     ARNRIRHDAIPALHYANPAAERSIARLCRQMRELDEWLTAEAAALLQAASVPGGYDAA
                     ALRSAAGPVLDAALHALVSPVRDAEEKYISLLRFLILKGEGAVQLTPEVTFKIRNGLL
                     VCLTKEGAQIAPAPPQPFEPGEFCLPGGYFVKFQVVKYEKFLKNQPIFKKDLNCCADC
                     AKIQGNVILRTRQPGDFYRPAGRHLRKTLKKYYNELGIPPAERPLLPLLADGSEVLWL
                     WGCGFAEGCAPDEYTHEVLTVRTEKTGEA"
     gene            1107..1649
                     /gene="hpt"
                     /locus_tag="ACZPAX_04485"
     CDS             1107..1649
                     /gene="hpt"
                     /locus_tag="ACZPAX_04485"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HIR1113188.1"
                     /translation="MDKDIDHVLVTEEQMKAKVAELGAQISRDYAGKDLLLVSILKGA
                     VVFMADLMREVKIPCAIDFMVVSSYGGANTSSTGLVKIIKDLDADLTGRDVLIVEDIL
                     DTGITLSKLVPVLKMRNPESVKICTILSKPSRRKADIEPDYCGFEVPDEFVVGYGLDY
                     DEKYRNLPYVGVLKPEVYSD"
     gene            1751..3787
                     /gene="ftsH"
                     /locus_tag="ACZPAX_04490"
     CDS             1751..3787
                     /gene="ftsH"
                     /locus_tag="ACZPAX_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HIR1113189.1"
                     /translation="MVLVLFFVSLSPLLAATGTKDTTYSEIYYYFENQQVTSFRLDLG
                     TGSLELWLKEGKAALPESNAAASLPSGGLLSLGGSEDEDLPANGGTVYMTYKLPYGGD
                     FNTGKISDFVDEYNEANPDAPMVFDYVAAKTSIPWLEIIFYVAMIASIGMLFMSMYRG
                     GAGGGIMNVGRAKVKDQQENQRKATFADVAGADEEKAELQEVVEFLKAPNKFNSLGAR
                     IPHGVLLVGPPGTGKTLLARACAGEAGVPFYAISGSDFVEMYVGVGASRVRDLFEKAK
                     KTMPSIIFIDEIDAVGRQRGAGLGGGHDEREQTLNQLLVEMDGFDANDGVIVMAATNR
                     ADILDKALLRPGRFDRQVYVGLPDVKGREEILRVHTKNKPLGPDVSLKTIARSTAGFS
                     GADLENLVNEAALLAARQGKKAITEKEIEEASVKVMMGPEKKSHVVTDEERRLTAYHE
                     TGHAITGYFCKHHDPVHQISIISRGGAGGYTMYLPEKDPSYVTKTAMFENIVSLLGGR
                     VAEQLVLDDISTGASNDLQRATDTARAMVTRYGFSERMGPVVYGSDPGETFLGRDFGQ
                     GKGYSETIASEIDNEIRDIVDEAYETARRTLTEHMDELHKVAGVLMEREKISGEEFKT
                     LMEGGTLPPYDIGKGETAKPEAPAPGNVEPVQPAEQTETQQPAEPDNSQPDTQE"
     gene            3804..4928
                     /locus_tag="ACZPAX_04495"
     CDS             3804..4928
                     /locus_tag="ACZPAX_04495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113190.1"
                     /translation="MDQNFSLMAQSRANYYTAGSPVQFVRVELLKGDVTGEVAVCLTF
                     KNVGTEPLTGLVVHFKCKDAAGQVLCEDDFYYEQLNAQPGAVFGSDDAVYVSDTPVSS
                     VEVEQDRAFLNGRGVDLRNYKRVRLNMPRVLPGSISRTLQQRTGNVQLTCVPQDTEYG
                     WFCACGAFHPNEENTTVCSECGGDRAAIKATLTGILEEARQAAERQQQEVNAVANSVA
                     PAPAPQLQAQPAPAATDPRAMANAVQAAITGQQDIPPQSAYEPEQATAAFEPQSNGYA
                     DDEDEEAERVRRYAPKGRLFDDEDDEDDGTQMYDTDDLDDDDYDDRKKSKKRGRYADD
                     DESSEDDVMAERIIRLAPPITAIACALIVAVSLVYHFILA"
     gene            complement(4973..5935)
                     /locus_tag="ACZPAX_04500"
     CDS             complement(4973..5935)
                     /locus_tag="ACZPAX_04500"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HIR1113191.1"
                     /translation="MKSFTAGPNENGVRLSRFVEGVTKDMPRSMMYKAFRNKRIKVNG
                     KRAEPDTRLQTGDLIEMYINDEFFPVGKPATKTAKPQRKQPPVTVVYEDANFAVLYKP
                     AHLLCHSDRTGDANLVDAFAAYLEGKGEYDPHAEKRFAPALCNRLDRGTEGLVLAAKS
                     YTALRDLNTIIRDDLMKKEYLTITVGAPPQGRFVAWLQHSEKNNKVRIHARESEGYKQ
                     IITEVTVIRQAGPFALCRIGLITGRTHQIRAHLAYLGHPVLGDIKYGNRKMNERSGLK
                     TQALCAQRLTFGRIPEENTLHYLSGKVIKLNDPEIIKTFERLTA"
     gene            6032..>6681
                     /locus_tag="ACZPAX_04505"
     CDS             6032..>6681
                     /locus_tag="ACZPAX_04505"
                     /inference="COORDINATES: protein motif:HMM:NF015935.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HIR1113192.1"
                     /translation="MAEENLHANHRARMQERVDRDGLDSLAEHEALEYLLFLSIPRAD
                     TNELAHRLINHFGDFCKVMEAEPDELMQVEGVGPKSARLIATVMAFGRYYQLKKRKTR
                     QALDKAEAAIEYVKPLFRGVQNEQLYLILLDDACRPVQDLRIAEGVPNRVTVDTRKLL
                     RAVARTNSTCGILAHNHPTGLAIPSEADRLTTYRIMEVTGQLGFTIMDHIIIAGEDG"
BASE COUNT         1409 a         2013 c         1957 g         1302 t
ORIGIN      
        1 ggaatcgaga taccgcccca tcccagtgag gattgggcgc gcggcctgcg gtacggctgg
       61 ttcgacgagc tggccgcgcg ggaggaagcc gttattgcca cggcgcacac gatgtccgac
      121 caggctgaga ccgtgctttt ccgcatggcg cggggaaccg ggctgcatgg actggcgggc
      181 atcccgcctc gcaggggctt ttatgtgcgg ccctgcctct gcctgacccg cgccgacacc
      241 gaggcatact gtgccgccct ggggcaacac tatatccagg acgagacgaa tgccgaggat
      301 acctacgccc gtaaccgcat ccggcatgac gccatcccgg cactgcacta tgcgaaccct
      361 gctgccgagc gcagcattgc cagactctgc cgccagatgc gtgaactgga tgaatggctc
      421 accgccgaag ctgccgccct gctgcaggca gccagtgtgc cgggcgggta tgacgccgct
      481 gccctgcgca gcgccgcagg cccggtactg gatgccgcac tgcacgccct tgtcagccca
      541 gtgcgcgacg ctgaggaaaa gtatatcagc ctgctgcgtt tcctgatttt gaaaggggaa
      601 ggggccgtcc agttgacccc ggaagtgacc ttcaaaatcc ggaacggact gctcgtctgc
      661 ctgacaaaag agggtgcaca gattgccccg gcaccgccac agccctttga acccggtgaa
      721 ttttgccttc cgggtggtta ttttgtgaaa tttcaggtcg taaagtatga aaaatttcta
      781 aaaaatcaac caattttcaa aaaagactta aactgttgtg cagattgtgc taaaatacaa
      841 gggaatgtta ttctgcgcac aaggcagccg ggggatttct accgcccggc tggccgccac
      901 ctgcggaaaa cactcaaaaa atattataac gagcttggca taccgcccgc cgaacgcccg
      961 ctgttgcctc tgcttgccga tggcagcgag gtactgtggc tgtggggctg cggctttgcc
     1021 gagggatgtg cccccgatga gtacacccac gaggtactga cggtgcggac agaaaaaaca
     1081 ggggaggcat aactctaatg aacagcatgg acaaagatat cgaccatgtt cttgtgaccg
     1141 aagagcagat gaaggccaaa gtcgccgagc tgggcgcaca gatcagccgc gattacgcag
     1201 gcaaagacct gctgctggtt tccattttaa aaggtgcggt cgtttttatg gcagacctga
     1261 tgcgcgaggt caaaatcccc tgcgctatcg attttatggt cgtctccagc tacggcggtg
     1321 ccaacacatc gtccacaggc cttgtcaaga tcatcaagga cctggacgct gacctgaccg
     1381 gcagggatgt tctgatcgtc gaagatatcc tcgacaccgg catcacgctg tccaagctgg
     1441 tcccggtgct caaaatgcgc aaccccgaaa gcgtaaagat ctgcaccatc ctcagcaagc
     1501 ccagccgccg caaagccgac attgagccgg actactgcgg ctttgaggtg cccgacgagt
     1561 tcgtggtcgg ctacggcctg gactacgacg aaaagtaccg caacctgccc tacgtgggcg
     1621 tactcaagcc ggaagtgtat tccgactgaa ccaaagatcc caaaatgccc ggtgaataca
     1681 ccggatagga gttgatttct tgcagcacaa acaaccgaaa ttcagcggca tccttgtggt
     1741 ggccgtcctg atggtgttgg ttctgttctt tgtcagcctt tcgccgctgc tggcggccac
     1801 cggcaccaaa gacacaacct actccgaaat ttattactac tttgaaaacc aacaggtgac
     1861 ctctttccgc ctggatctgg gcaccggcag cttggaactt tggctcaagg aaggcaaagc
     1921 cgccctgccg gagtccaacg ccgcagccag cctgcccagc ggcggcctgc tgagcctcgg
     1981 cggttcggag gacgaggatc tgcccgccaa cggcggcacg gtctatatga cctacaagct
     2041 gccctacggc ggtgacttca acaccggaaa aatctccgat tttgtcgatg aatacaacga
     2101 ggccaacccc gacgccccga tggtgttcga ctatgtcgcc gccaagacaa gcattccctg
     2161 gctggaaatt atcttctatg tcgctatgat tgccagcatc ggcatgctgt tcatgtcgat
     2221 gtaccgcggc ggcgcaggcg gcggcatcat gaatgtgggc cgcgccaagg tcaaggatca
     2281 gcaggagaac cagcgcaagg caacctttgc cgatgtggcc ggtgccgatg aggaaaaggc
     2341 cgagctgcag gaagtcgttg agttcctgaa agcgcccaac aaattcaact ctctgggtgc
     2401 gcgcatcccg cacggcgtgt tgctcgtcgg ccctccgggt accggtaaga ccttgctggc
     2461 tcgtgcctgc gccggtgagg ctggcgtgcc gttctacgcg atttccggct ctgacttcgt
     2521 cgagatgtac gtcggtgttg gtgcctcccg cgtgcgtgac ctgtttgaaa aagccaaaaa
     2581 aacaatgccc tccatcatct ttatcgatga gatcgatgct gtaggccgtc agcgcggcgc
     2641 aggtctcggc ggcggccacg atgagcgtga gcagaccctg aaccagctgc tggtcgaaat
     2701 ggacggtttt gacgccaacg acggcgttat cgttatggcc gccacgaacc gtgcggatat
     2761 cctggataag gcgttgctgc gcccgggccg ttttgaccgc caggtctatg taggcctgcc
     2821 cgacgtcaag ggccgcgagg agatcctccg ggtccacacg aagaataagc ccctcggccc
     2881 cgatgtgagc ctgaaaacga tcgcccgctc caccgccggt ttctccggcg cggatttgga
     2941 aaacctcgtc aacgaggcgg ccctgctggc tgcccgccag ggcaagaagg ccatcaccga
     3001 gaaggagatc gaggaagcct ccgtcaaggt catgatgggc cccgagaaga agagccacgt
     3061 tgtcaccgac gaggagcgcc gcttgaccgc ttaccacgag acaggccacg ccatcaccgg
     3121 ctacttctgc aagcaccatg acccggtgca ccagatcagc attatctccc gcggtggtgc
     3181 aggcggctac acgatgtacc tgcccgagaa agaccccagc tacgtcacca agactgcaat
     3241 gttcgagaac atcgtctcgt tgctgggcgg ccgtgtggcc gagcagcttg tgctggatga
     3301 tatctccacc ggcgcatcca acgacctgca gcgcgccacc gatacggccc gcgccatggt
     3361 cacgcggtac ggcttctcgg agcggatggg gcctgtcgtc tacggcagcg accccggtga
     3421 aaccttcctg ggccgtgact tcggccaggg caagggctac tccgagacga ttgcctccga
     3481 gatcgacaac gagatccgtg acattgtgga cgaagcctac gagaccgccc gccgcaccct
     3541 gaccgagcac atggacgagc tgcacaaggt cgcaggcgtc ctgatggagc gcgagaagat
     3601 cagcggtgag gaattcaaga ccctgatgga gggtggaacc ctgccgccct atgacatcgg
     3661 caagggcgaa accgccaaac ccgaagcacc tgcgccggga aatgtagaac ctgtgcagcc
     3721 tgctgaacag accgaaaccc agcagccagc cgagccggac aactcccagc ccgacacgca
     3781 agagtaaaga gtaggtgtaa actatggatc agaatttttc cctgatggcg cagtcccgcg
     3841 ctaactatta cacggccggc agcccggtcc agtttgtgcg tgttgagctg ctgaaaggcg
     3901 acgtgaccgg tgaggtcgcc gtctgcctga cctttaaaaa tgtcggcacc gagccgctga
     3961 ccggcttggt cgtccacttc aaatgtaagg atgccgccgg tcaggtcctg tgcgaggatg
     4021 acttctacta tgagcagctc aacgcccagc ccggtgctgt gttcggcagt gacgatgccg
     4081 tctatgtcag cgacacgccg gtctccagtg tcgaagtcga gcaggatcgc gcgttcctga
     4141 atggccgtgg cgttgacctg cgcaactaca agcgcgtgcg cctgaatatg ccccgtgtgc
     4201 tgcccggctc catttcccgc actttgcagc agcgcaccgg caatgtgcag ctgacctgtg
     4261 tgccccagga taccgagtac ggctggttct gcgcctgcgg cgcgttccac cccaacgagg
     4321 aaaacaccac tgtctgctcc gagtgcggcg gtgaccgcgc agccatcaag gccacgctga
     4381 ccggcatttt ggaggaagcc cgccaagctg ccgagcgcca gcagcaggaa gtcaacgctg
     4441 ttgcaaacag cgttgcgccc gccccggccc ctcagctcca ggcacagcct gcccctgccg
     4501 ctactgaccc ccgcgctatg gccaacgccg tgcaggccgc tatcacgggt cagcaagata
     4561 tcccgccgca gagtgcctac gagccggaac aggctaccgc cgcctttgag ccacagagca
     4621 acggttatgc ggatgatgag gacgaggaag ccgaacgcgt ccgccgttac gcccccaagg
     4681 gccgtctgtt cgacgacgag gatgacgagg acgacggcac ccagatgtac gacaccgacg
     4741 atctggacga cgacgattac gatgatcgca agaagtccaa gaagcgaggc cgttacgccg
     4801 atgacgacga gtccagcgaa gatgacgtca tggccgagcg tatcatccgc ttggccccgc
     4861 ccatcacggc catcgcctgc gcactgattg ttgcggtatc cttggtctat catttcattt
     4921 tggcataaaa aattgccgtg gcattccgct tttggaatgc cacggcaatt ttttatgccg
     4981 tcaaacgctc gaacgtctta atgatttccg gatcattcag cttaatgacc ttgccggaaa
     5041 ggtagtgcag tgtattttcc tcggggatgc ggccaaacgt cagccgctgg gcacacaacg
     5101 cctgggtctt tagcccactg cgctcgttca tcttgcggtt gccgtacttg atatcgccca
     5161 gtactgggtg gcccaggtaa gccaggtggg cgcgtatctg gtgggtacgc ccggtaatca
     5221 gtccgatgcg gcacagcgca aacggccctg cctggcggat gaccgtcact tcggtgatga
     5281 tctgcttgta gccctcgctc tcccgcgcat ggatgcgcac cttgttgttt ttttcgctgt
     5341 gctgcagcca ggccacaaac cggccctggg gcggggcccc caccgtaatg gtcaggtatt
     5401 ccttcttcat taagtcgtcg cggatgatgg tgttcagatc gcgcagggct gtgtaactct
     5461 tggcggccag caccaatcct tccgtgccgc ggtccagacg gttgcacagt gccggggcaa
     5521 accgtttttc ggcgtggggg tcatactcgc ccttgccctc taaatatgcg gcaaaggcat
     5581 ccaccaggtt ggcatccccg gtgcggtcgc tgtggcagag caggtgcgcg ggcttgtaca
     5641 gcaccgcaaa gttggcatcc tcgtacacaa cggtcacagg gggctgtttg cgctgcggct
     5701 tggcagtctt ggtggctggc ttgcccaccg ggaaaaactc gtcattgatg tacatttcga
     5761 tcaaatcgcc ggtctgcagc cgggtgtccg gctcggcgcg tttgccgttg actttgatgc
     5821 gtttattgcg gaacgccttg tacatcatgc tgcggggcat atctttggtt acgccctcca
     5881 caaagcggct caggcgcacg ccgttttcgt tagggcctgc tgtaaaactt ttcatcgtat
     5941 cctctttaaa aatcatgcgt aaaacagttg tctaaatgct ctattcagta tataataaaa
     6001 ccagcaaaac gtaaagatgg gatgtgaaaa aatggcagag gaaaatctcc acgcgaacca
     6061 ccgcgcccgg atgcaggagc gcgttgaccg cgacggtctg gacagcctgg ccgagcatga
     6121 ggcgctggaa tacctgctgt ttttgtctat cccgcgggca gacaccaacg aattggccca
     6181 tcggctcatc aaccactttg gggatttctg caaggtgatg gaggccgaac ccgacgagct
     6241 gatgcaggtt gagggggtcg gccccaagag tgcgcggctg attgccacag ttatggcgtt
     6301 cggccggtac taccagctga aaaagcgcaa gacccgccag gctcttgaca aggccgaggc
     6361 ggccattgag tacgtgaaac ccctgttccg cggcgtgcag aatgagcagc tgtatttgat
     6421 tctgctggac gacgcctgcc gaccggtgca ggatctgcgc atcgccgagg gtgtgcccaa
     6481 ccgcgtgacg gtggacactc ggaagctgct gcgcgccgtg gcccgcacca actctacctg
     6541 cggcatcctg gcccacaacc atcccaccgg gttggccatc ccctccgaag ccgaccgcct
     6601 gacgacctac cgcattatgg aagtcaccgg ccagctgggc tttactatta tggatcatat
     6661 catcatagca ggggaggacg g
//
LOCUS       DBMDSQ010000086         6638 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_2979_length_6638_cov_2.114426, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000086 DBMDSQ010000000
VERSION     DBMDSQ010000086.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 6638)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6638)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6638
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2979_length_6638_cov_2.114426"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..946
                     /locus_tag="ACZPAX_04510"
     CDS             <1..946
                     /locus_tag="ACZPAX_04510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113193.1"
                     /translation="STAPEGDWQKALLNSGVTAIIDGTTAESSPNVLGAGLGLDGVAQ
                     LNLVFTQGGGCRAEVQSAVTADTLQAKRTDWETLAASAAADDQTTASDAADPDKDTEA
                     AGGKDTAAPTESVDEAQQAGADAYIYAAAKLAGLDADDQSIYYTPLFTYAEKPDTSKT
                     ISFGNYLAALYAEIVANDNAGGLPEGTSAEAFAGGVTELEYGDITRGDLLNALPATAR
                     IQLVSLPADAAKALADGGTVSRVYQNSLTEYAPEGDTAYIVTDTATLAALNVDYTVLR
                     DYGDVFWAVRMNINDLTNNFNDDFVLPEAPQYGVGRRG"
     gene            1454..3076
                     /locus_tag="ACZPAX_04515"
     CDS             1454..3076
                     /locus_tag="ACZPAX_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase RNA specificity
                     domain-containing protein"
                     /protein_id="HIR1113194.1"
                     /translation="MKLTFLGADREVTGSCTQVEAAGHRFLVDCGMEQGRDVYENADL
                     PYAPGTYEALLLTHAHIDHSGRIPYLYKNGYRGPVYTTEATRDLCGIMLEDSAHIQEQ
                     EAEWKNRKAMRSGAEMIEPDYTVEDAQNVMKQFVPCPYEAWQTLFDGPQGRIEVRFTD
                     VGHLLGSASISLRIAEPGRAPEIIVFSGDIGNQHQPLIKDPANPGHADYLVMESTYGD
                     RSHGPKPDYLGELTAVLQSTFDKGGNVVIPSFAVGRTQELLYFFRQIKAEKRVKGHDN
                     FPVFVDSPLASKSTTIFRENFAECYDDEALALVQSGRNPLQFPGLHISETKEESMAIN
                     GDPTPKVIISAAGMCDAGRIRHHLKYNLWRPECTVLFVGYQSPGTLGNALVNGAQEVK
                     LFGEPVQVKARIAQLGGLSGHADCDGLAAWLHAFDEKPKFVFVNHGEDAVAEHWVEKL
                     RTEGYEADAPYNGSVWIAAEGRVACAETGNTQKIIRTKSAEMVRTSAAYDRLVNMGKR
                     LMEVIRHNQGGANKDLAKFASQIAALCDKWDR"
     gene            3203..3742
                     /locus_tag="ACZPAX_04520"
     CDS             3203..3742
                     /locus_tag="ACZPAX_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004602404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5662 family protein"
                     /protein_id="HIR1113195.1"
                     /translation="MWHPIKHYKTIHHHKMLVMKHCFRCGLYWQGLTHDLSKLAPVEF
                     WAGAKYWQGTCSPNNAQRQTEGYSAAWLHHKGRNKHHLEYWIDYAPDGDHAMAGMRMP
                     EKYVAEMVCDRIAASKNYKGSRYTDAAAWEYYDRSRDHYILHPETRKQLETCLLILRD
                     KGEDACFAHIRKELLGKKK"
     gene            complement(3815..4693)
                     /locus_tag="ACZPAX_04525"
     CDS             complement(3815..4693)
                     /locus_tag="ACZPAX_04525"
                     /inference="COORDINATES: protein motif:HMM:NF024242.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1113196.1"
                     /translation="MINTTLPQSNTVPACTALGSGVWLAFRHIAGTSAPAVPPPAAGA
                     HLLSVEFCRRGRHALPAGDYCTIAADGHAAVSMTAAGADDYYPGAVYEGLQFWIDPDA
                     QTDSGFLALMGLDVHGIINYFCAGGPYYCPMGDELAALVDELWREFDVSGYASPGELR
                     YAVVRLLYMLLQLPYADQMAWYPRAQVDLVRDAETLMLADLSVRHTARELAVQFGVSE
                     SSFKAYCRDVLGDGYLPYFRRKRLEKAADLLAHTDLKVQDIAVQVGYESQSKFAKAFA
                     DQFRLTPLEYRRLAEK"
     gene            complement(4897..5814)
                     /locus_tag="ACZPAX_04530"
     CDS             complement(4897..5814)
                     /locus_tag="ACZPAX_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015564312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HIR1113197.1"
                     /translation="MSKTYLGVDIGGTAVKLGLVDDTGRVLRRAERSVSFDGYKTPIL
                     DTVQTAIRDFLGSHDVPKLEGIGVSATGQIDSRRGVVAGTCGNFPGWIGVDIKGTLER
                     EFGLPVTVANDANCMVLGEVWVGAAKGYTDVIGVTLGTGVGGGIITGGRLLEGARGLG
                     GELGHFRLHALDGVACTCGAIGCYERYAATTALVRGAQKMGLDAPDGRAIFEAAAAGN
                     ARTLAVLNDWISEIAQGLAGLVHIFNPQLILIGGGVSAQQELLIDPLAAQVCKSVMPA
                     FAEGLEIKAAALQNDAGLVGAVYYFRENQ"
     gene            complement(5826..6527)
                     /locus_tag="ACZPAX_04535"
     CDS             complement(5826..6527)
                     /locus_tag="ACZPAX_04535"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047465 - N-acylglucosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HIR1113198.1"
                     /translation="MTHEQIFTQIQGGLIVSCQALEHEPLYTKEGGVMPLMAKAAAQS
                     GAVGIRANTVRDITQIKQVVDLPVIGIIKKDYPGTPMYITVTMQEVDELVACGVDILA
                     VQGTGALRPNGQTSAEFIRAIKEKYPDQLVMADCDNIENALACAGAGADFVGTTMRGY
                     TPETKGINDVDFGFIAQLARECPAKVIAEGHIHTPEQAVQALYAGAFALVVGGAITRP
                     AEITARYTAAINNHK"
BASE COUNT         1347 a         2107 c         2038 g         1146 t
ORIGIN      
        1 gtccaccgcg ccggagggtg actggcagaa ggcgctgctg aacagcggcg tgaccgccat
       61 catcgacggc acgaccgccg agagcagccc gaacgtgctg ggcgcgggcc tgggcctgga
      121 cggcgtggcg cagctgaatc tggtgttcac ccagggcggc ggctgccgcg ccgaggtgca
      181 gagcgccgtg acggcggaca ccttgcaggc aaaacgcacc gactgggaaa ccctggccgc
      241 cagtgctgcc gccgacgacc agaccacggc aagtgatgct gccgaccccg acaaggacac
      301 cgaggccgca ggcggcaagg acaccgccgc cccgaccgag agcgttgacg aagcgcagca
      361 ggcgggtgcg gacgcctaca tttacgccgc cgccaagctg gccgggctgg acgccgatga
      421 ccagagcatc tactacacgc cgctgttcac ctacgccgaa aaacccgaca ccagcaagac
      481 catcagcttc ggcaattatc tggcggcgct ctacgctgaa attgtggcaa acgataacgc
      541 cggcggcctg cccgagggca catccgccga agcctttgct ggcggcgtga ccgagctgga
      601 atacggcgat atcacccgcg gcgatctgct gaacgccctg cctgccacgg cgcgcatcca
      661 gctggtcagc ctgcccgctg acgcggccaa ggccctggcc gacggcggca cggtgagccg
      721 cgtttaccag aacagcctga cagagtacgc gcccgagggc gataccgcct acatcgtcac
      781 cgacaccgcc acgctggcag ccctgaatgt tgactacacc gtcctgcgcg actacggcga
      841 cgtattctgg gctgtacgca tgaacattaa cgatctgacg aataatttca atgatgactt
      901 tgtcctgccc gaggccccgc agtacggcgt cgggcggcgc ggctaaagga tgaacggcat
      961 cctcccggga ggtcgctttc ttttggcagc aaaagaaagc tcgcaaagaa aactgccgct
     1021 ctttcgacgg agcggacccg cgacctaggg gctgcacgcc cctaggaacc ccaaagcgga
     1081 agtcgcaagg cccttcggct acgcgaacgc agtattactg ttcgcgctta acgcctacgg
     1141 gccttgctcc acttttgagt cggggagacc ccgaaccccc gctgtgggaa gtgcatccgt
     1201 aggggtgggg cttctgctcc gcccacgctg tcgggggagg gctgccgccc tcccttgccc
     1261 ctctcacttt tcctctgctg ctccgtaggg cggccagccc tctggccgcc gcacctttct
     1321 catggcacga cttggcactt tagttgtagg gggcggcgtc ctcgacgccc cgcatcgcac
     1381 cttgtccaat atccccatac cccacccgcc gctcaccaaa catatggatt atttgaccaa
     1441 agggggcacc cacatgaaac tcacattcct cggcgctgac cgggaagtca ccggcagctg
     1501 cacacaggtc gaagccgcag ggcaccgctt tttggtggac tgcggcatgg agcaggggcg
     1561 cgacgtctac gaaaacgccg acctgcccta tgcccccggc acctatgagg ccctgctgct
     1621 gacccatgcc catatcgacc actcggggcg catcccgtac ctgtacaaaa atggctaccg
     1681 cggcccggtc tataccaccg aggccacccg cgacctctgc ggcatcatgc tcgaggactc
     1741 cgcccacatc caggagcagg aggccgagtg gaaaaaccgt aaggccatgc gcagcggcgc
     1801 ggaaatgatc gagccggact acacggtcga ggacgcccaa aacgtcatga agcagttcgt
     1861 gccctgcccc tatgaggcat ggcagaccct gttcgacggc ccccaggggc gtatcgaggt
     1921 gcgctttacc gatgtgggcc acctgctggg cagcgcgtcc atctcgctgc gcattgcgga
     1981 gccgggccgt gcgccggaaa tcatcgtgtt ttccggcgat atcggcaacc agcaccagcc
     2041 gctcatcaag gatccggcca accccggcca tgccgattac ctcgtcatgg agtccaccta
     2101 cggcgaccgc agccacggcc ccaagcccga ctacctgggc gagctgaccg ccgtgctgca
     2161 atcgaccttt gacaagggcg gcaacgtggt catccccagc tttgccgtgg gccgcacgca
     2221 ggaattgctc tatttcttcc gccagatcaa ggccgaaaag cgcgtcaagg gccacgacaa
     2281 tttcccggtg ttcgtggaca gcccgctggc cagtaagtct accacgattt tcagggaaaa
     2341 cttcgccgag tgttacgacg acgaggccct tgccctggtg cagagcggcc ggaacccgct
     2401 gcagttcccg gggctgcaca tcagcgagac gaaagaggaa tccatggcca tcaacggcga
     2461 cccgacgccg aaagtcatta tctccgctgc cggtatgtgc gatgcgggcc gcatccgcca
     2521 tcacctgaaa tacaacctct ggcggccgga gtgcacggtg ctgttcgtcg gctaccaaag
     2581 cccgggcacc ctgggcaatg cgctggtcaa cggcgcacag gaggtcaagc tcttcggcga
     2641 gccggtgcag gtcaaggccc gcattgcgca gctgggcggg ctttcgggcc atgcggactg
     2701 tgacggtttg gccgcctggc tccacgcctt tgacgagaaa ccgaagttcg tttttgtcaa
     2761 tcatggcgag gacgctgtgg ccgagcactg ggtcgagaaa ctgcgcaccg aaggctacga
     2821 ggccgacgcg ccgtacaacg gctcggtctg gatcgcggcc gaagggcgcg ttgcctgcgc
     2881 cgagacgggc aacacgcaga agatcatccg caccaagagc gccgagatgg tgcgcaccag
     2941 cgctgcctac gaccggctcg tcaacatggg caagcgcctg atggaggtca tccgccacaa
     3001 ccagggcggc gccaacaagg accttgccaa attcgccagc cagatcgccg cactgtgcga
     3061 caaatgggac agataaaaaa gataatagat aagaaaattg gcggatcgca ccgcttgcgc
     3121 ggcgctcaaa atttcgtttt cgcccttggg gcgaaaatac cacatttttt attatctatt
     3181 atctactaaa ggagtattta ctttgtggca tccgatcaag cattataaaa cgatccatca
     3241 tcataaaatg ctggtgatga agcactgctt ccgctgcggg ctgtactggc aggggctgac
     3301 ccatgatttg agcaagctcg cgccggtgga gttctgggcc ggtgccaagt actggcaggg
     3361 cacttgcagc cccaacaacg cccagcgcca gaccgaagga tacagcgcgg cctggctgca
     3421 ccacaagggc cggaacaagc accacctgga atattggatc gactacgccc cggacggcga
     3481 ccatgccatg gccgggatgc ggatgcccga aaaatacgtg gccgagatgg tctgcgaccg
     3541 tattgccgcc agcaaaaact acaagggcag ccggtacacc gatgccgctg cgtgggagta
     3601 ctacgaccgc agcagggatc actacatcct gcaccccgag acccgcaaac agctggaaac
     3661 ctgcctgctg attttacgcg acaagggcga ggacgcgtgc tttgcgcata tccgtaagga
     3721 actgctggga aagaaaaagt aagcaatgcg gtctataaac aacaaaagcc ccgtgggcac
     3781 agtttttaca ctgtgcccac ggggaaagct ttttttattt ctccgccagc cgccggtatt
     3841 ccagtggggt caggcggaat tggtcggcaa aggctttggc aaatttgctc tggctctcgt
     3901 aaccgacctg tacggcgatg tcctgtacct ttaagtccgt gtgggccagc aggtctgcag
     3961 ccttttccag ccgcttgcgg cggaagtagg gcaggtagcc gtcgcccagc acgtcgcggc
     4021 agtaggcttt gaagctgctc tcgctgacgc cgaactgcac ggccagctcc ctggcggtgt
     4081 ggcgcaccga caaatctgcc agcatcaggg tctcggcatc gcgcaccaaa tccacctgcg
     4141 cccggggata ccaggccatc tggtccgcgt aaggcagctg cagcagcata tagagcagcc
     4201 gcaccacggc atagcgaagc tcgcccgggc tggcatagcc cgaaacgtcg aattcgcgcc
     4261 aaagctcgtc caccagcgcg gcaagttcgt cacccatggg gcagtaatag ggaccgccgg
     4321 cgcagaaata gttgatgatg ccgtggacgt ccaggcccat cagggccaga aagccgctgt
     4381 cggtctgggc gtccgggtcg atccagaatt gcagcccctc ataaacggcc ccggggtagt
     4441 agtcgtcggc cccggcggcg gtcatgctca cagcggcgtg gccgtcggcg gcgatggtgc
     4501 agtagtcccc ggcaggcagc gcatggcggc cccggcggca gaactccacc gagagcaggt
     4561 gtgccccggc ggcgggcggc ggcacggcgg gggccgatgt gcccgcgata tgccggaacg
     4621 ccagccacac cccggacccc agcgctgtgc aggcggggac ggtattcgat tgtggtaatg
     4681 ttgtgtttat catggcagta ttatagcaca agatgatgtg tgcggcaaga ggttttaaag
     4741 aaataagatg ctaaaagggg ctaaatacgc aaatttgcgt ttttgcatag gcaatttaaa
     4801 tgctaaaaag actaaaagtg catagctgtc aaaatgacga aaaacacccc gggaacacac
     4861 gccggattcg gcaatgtgcc cggggtgatg ttgtggttat tgattttccc ggaaataata
     4921 cacggcaccg accagaccgg cgtcgttttg gagggcggcg gctttgattt ccagcccctc
     4981 cgcgaaagcg ggcatgaccg acttgcacac ctgcgcggcc agcggatcga tcaaaagctc
     5041 ctgctgggcg ctgacaccgc cgccaatcaa aatcagctgc gggttgaaga tgtgtaccag
     5101 cccggccagg ccctgcgcaa tctcgctgat ccagtcgttc agcacagcca gcgtgcgggc
     5161 gttacctgcg gcggctgcct caaaaatcgc gcggccgtcg ggggcgtcca ggcccatctt
     5221 ctgggcaccg cgcaccagcg ccgtcgtggc ggcgtaccgc tcatagcagc cgatggcacc
     5281 gcaggtgcag gccacgccat ccagcgcgtg caggcggaaa tgtcccagct caccgccaag
     5341 gccccgcgca ccctccaaca aacggccgcc ggtgatgatg ccgccgccca cgccggtgcc
     5401 taaggtgaca ccgatcacat cggtgtaccc cttggccgcg ccgacccaga cttcgcccag
     5461 caccatgcag ttggcgtcgt tggcgacggt gaccggcagg ccaaactcgc gttccagcgt
     5521 gcctttgata tccacgccga tccaaccggg gaaattgccg caggtccccg cgaccacgcc
     5581 gcggcggctg tcgatctgcc cggtggcgga cacgccgatg ccctcgagct tcggcacatc
     5641 atggctgccc aaaaagtcgc ggatggcggt ctgcacggtg tccaggatcg gcgtcttgta
     5701 gccgtcaaag ctgacgctgc gctcggcgcg gcgcagcacg cggccggtgt catcgaccag
     5761 gcccagcttg accgccgtgc cgccgatatc gacgcccaga taggttttac tcatagcgca
     5821 cctgcttatt tgtggttgtt gatggcggca gtgtagcggg cagtgatctc ggccgggcgg
     5881 gtgatggcac cgcccacgac cagcgcgaac gcaccggcgt agagggcctg cacggcctgc
     5941 tcgggggtgt ggatgtggcc ctcggcaatg accttggccg ggcactcgcg ggccagctgg
     6001 gcgataaagc cgaagtcaac gtcgttgatg ccctttgtct cgggggtgta gccgcgcatc
     6061 gttgtaccga cgaaatccgc accggctccc gcgcaggcca gcgcattctc aatattgtcg
     6121 cagtcggcca taacgagctg gtcggggtac ttttccttga tggcgcggat aaattcggcg
     6181 ctggtctgcc cattggggcg cagggcaccg gtgccctgca cggccagaat gtccacgccg
     6241 caggccacca gctcgtcaac ctcctgcatc gtgacggtga tgtacatcgg cgtgccgggg
     6301 taatcctttt taataatgcc gatgaccggc aggtcaacga cctgcttgat ctgggtgata
     6361 tcgcgcacgg tgttggcgcg gatgccgaca gcaccggact gggcggcggc cttggccatc
     6421 agcggcatga cgccgccttc tttcgtgtac agcggttcgt gctccaatgc ctggcaggag
     6481 acgataagac cgccctggat ttgggtgaag atttgttcgt gggtcatagt gaggactcct
     6541 ttccatacgc aaaaaggctt ccccctcggg ggaagctgtc accgaaggtg actgatgagg
     6601 gcggagctta cggcggcaac ccgtttacgg gcaaccat
//
LOCUS       DBMDSQ010000087         6615 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_2990_length_6615_cov_1.802272, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000087 DBMDSQ010000000
VERSION     DBMDSQ010000087.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 6615)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6615)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6615
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2990_length_6615_cov_1.802272"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..671
                     /locus_tag="ACZPAX_04540"
     CDS             <1..671
                     /locus_tag="ACZPAX_04540"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007045855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HIR1113199.1"
                     /translation="TLMTMSEIIEDELTKAKRTARVVALSGPTHAEEVARDMPTAIVA
                     ASADEDAAKTVQKIFNTPTFRVYTNDDRRGTELGGAVKNVIALAVGIALGLGYGDNAK
                     AALITRGNAELSRLGIAMGCKPETFAGLSGMGDLIVTCTSMHSRNLHAGMLIGRGKSV
                     EDAKTEVGQVVEGINALPAACHLAKKYKVEMPIVQAVEAILDGKLEARNALMALMGRN
                     LKRE"
     gene            676..1458
                     /locus_tag="ACZPAX_04545"
     CDS             676..1458
                     /locus_tag="ACZPAX_04545"
                     /inference="COORDINATES: protein motif:HMM:TIGR00099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HIR1113200.1"
                     /translation="MADIKAAFFDIDGTLLPFEAKHLPASTVDALARLRKNGVKTFIA
                     TGRPPVHLPYLHALDGTPFDGYVTMNGQYCYLADGELLYSKYIPAASLQTLLPYIEKE
                     QLSVGFVGKDFCRFNLINDLSRDFAKKLELGAGDVAALIQHEDIYQLSAFLPPEKEAE
                     FLRRCPGCLAVRWEDDFCDILPEGGGKPNGLAHTLAHLGLTREQCIAFGDGGNDVTML
                     EYAGIGVAMGNACDTAKQAADYVTDDIMKDGLAKALGHFHLI"
     gene            complement(1600..2583)
                     /locus_tag="ACZPAX_04550"
     CDS             complement(1600..2583)
                     /locus_tag="ACZPAX_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006356390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1113201.1"
                     /translation="MIFADKLITLRKKAGWSQEELAEKLNVTRQSVSKWEGAQSVPDI
                     DKILQLSCLFGVTTDYLLKDDAAEPEYTEDDTSPLPRVTLAQANDYLAKAKANAPRLA
                     LAAALCVVSPIPLLALVALSEAGLFGIWDDLAGGIGLIALLVIIAAAVAIFMQCSASV
                     RAYEFLQKQAIETEYGVTGLAKERRDAFAPQYDKANILGTVLCVLSAVPVFAAMMVGA
                     SFLMSASICLVLALVACGVYAFVRVGTVRDAMDQLLEEGDYTRPNKAIKSRINALTAA
                     YWLVVVAIFLWYTFGPQGNGQPQYSWFIWAIGGVVYAALVIAVKAFMKKNR"
     gene            complement(2666..3388)
                     /locus_tag="ACZPAX_04555"
     CDS             complement(2666..3388)
                     /locus_tag="ACZPAX_04555"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:NF003588.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HIR1113202.1"
                     /translation="MLLTAELDRLSATDWQPFFAQERAKPYFAELDAFVTAAAAEKTV
                     YPAPENIFAAFRACPASSMRVVILGQDPYHEPGQAMGLSFSVPDGCKAPPSLRNIFKE
                     LESEFGPGCAAHTDLTPWAQQGVLLLNTVLTVEQGAANAHASHGWETFTRAALEYAAA
                     AGTAPLAAVLWGKPAQKYAAIFEKAAARRPVLVLESAHPSPLSAYRGFFGSAPFGKVN
                     AFLENNGVSKIDWCLPANATQS"
     gene            3633..4772
                     /locus_tag="ACZPAX_04560"
     CDS             3633..4772
                     /locus_tag="ACZPAX_04560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113203.1"
                     /translation="MRSIKTKLFSLAMAVSMVLALAGCAMSTPSTVGSIGGIEIPAGI
                     YLLAQYNSYNTAANAAELATSETASDVKAVLKATCTGTIGDEEVTATGSEYVAKLTDR
                     AISYYAEVERRFTEMNGVLDDAATAEAADTADNLWSTNGDLYTANGISKTSLQTYLLN
                     AQKAKALLKMTYGPDGTTPVAEDEYTDFVNNDCYYIEAVQFPLVDYSTYSMADDDQKA
                     AIMATAESCMAELNTTATAETASNSALYTAAMTYVPQAMAAMGSSLDASQAVYYAASQ
                     LYTPDDLSSYGSDEYNNLTDPLDEAGLNHWTTINLGTTILVARKIDPFKTYTVDELNS
                     MYDLLSSMKSTDIQSELYAAGAALEHNLNSSALNTYSASKIKKNV"
     gene            4838..5710
                     /locus_tag="ACZPAX_04565"
     CDS             4838..5710
                     /locus_tag="ACZPAX_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HIR1113204.1"
                     /translation="MTKNKSVNFLLQMLGCFISAAGIYSFAVASEVPVTGVAGICAIL
                     YRLFGIPMGLSNVLINIPIILCTYKLLGKSFFIRSVYCMVLFAVFTDYLLPLMPVYQG
                     DRLLATICGGVVGGIGDALIYMNNSSTGGLDFITMGIKAKHPHLPFGNITFAAALAVI
                     LLNGAVFQDVDSIIYGIMFNFIVSAVINKMMFGFSSSMLAMIITDDGAAACAEIDRVA
                     DRGSTILQGRGGYGGQPKDVVLCACSNKQLYEIEHAVQAIDPGCFIIMLQSNEVHGEG
                     FRQLELGKNENKSE"
     gene            5818..>6615
                     /locus_tag="ACZPAX_04570"
     CDS             5818..>6615
                     /locus_tag="ACZPAX_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009003027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1113205.1"
                     /translation="MDATKIGTFIAAERRAKGWTQRQLADKLQLTDKAVSRWETGKGL
                     PDVSFLLPLAAALDVSVGELLAGESQPQPPAVQAAETRTAEQLASYTRELGPQLRRRR
                     ACTVLAGLLLFAAAFLSLQFLRLLMGGAGIHGLSVTSLLLLVVLPFVFATFAFWLLHL
                     LCTDGLAETRAFRRASAMAVAGLWLFDLYFATAFSGCAAFPVLDFWRAPSWQANVNLV
                     PFRQIMQYLLCLVPGQGYLNGGGYAFRGHFAFWMNFFVFSVPVLLLPR"
BASE COUNT         1316 a         2050 c         1936 g         1313 t
ORIGIN      
        1 agaccctgat gaccatgagc gagattattg aggacgagct caccaaggca aagcgcacgg
       61 cccgggtcgt ggcgctgtcc ggccccaccc acgccgagga agtcgcccgc gatatgccca
      121 ccgccatcgt ggccgccagc gccgacgagg acgccgccaa gactgtgcag aaaattttca
      181 acacaccgac cttccgcgtc tataccaacg acgaccgccg cggcaccgag ctgggcggcg
      241 cggtcaagaa cgtcattgcg ctggccgtcg gcatcgcgct gggcttgggt tacggcgaca
      301 acgccaaggc cgcccttatc acccgcggca acgccgagct ttcccgtctg ggcatcgcca
      361 tgggctgcaa gcccgagaca tttgcgggcc tgtccggcat gggcgatctg atcgtcacct
      421 gtaccagtat gcacagccgc aacctgcacg ccggtatgct gattggccgc ggcaaatccg
      481 tcgaggacgc caagactgag gtcggccaag tcgttgaggg catcaacgcg ctgcccgccg
      541 cctgccacct tgccaaaaag tacaaagtcg aaatgcccat cgtgcaggct gtggaggcca
      601 tcctcgacgg caagctcgaa gcccgcaatg ccctgatggc gctgatgggc cgcaatctga
      661 agagggagta accccatggc ggatatcaaa gctgcatttt ttgacattga cggtacgctg
      721 ctgccttttg aggccaagca cctgcccgcc tcaacggtgg acgcgctggc ccggctgcgc
      781 aaaaacggcg tcaaaacgtt tattgctacc ggccgcccgc cggtacatct gccgtacctg
      841 cacgcgctgg acggcacccc cttcgacggc tatgtcacga tgaacggcca gtactgctac
      901 ctggccgacg gcgagctgct gtacagcaag tatatcccgg cggcatcgct gcaaacgctg
      961 ctgccgtaca ttgaaaaaga acagctgtcc gtcggttttg taggcaagga cttctgccgg
     1021 ttcaacctta tcaacgattt gagccgtgac tttgccaaaa agctggagct gggggctggg
     1081 gatgttgccg cgctgatcca gcacgaggat atttaccagc tcagcgcgtt cctgccgccg
     1141 gagaaggagg ccgagttcct gcgccgctgc cccggctgcc tggctgtgcg ctgggaggat
     1201 gatttttgcg atatcctacc cgagggcggc ggcaagccta acggcctggc ccacacgctg
     1261 gcgcacctgg ggctgacccg ggaacagtgc atcgccttcg gtgacggcgg caacgatgtg
     1321 acgatgctgg aatacgcagg catcggcgta gcgatgggca acgcctgtga taccgccaag
     1381 caggccgcgg attatgtcac ggatgatatt atgaaagacg gcctggcgaa ggcgttgggg
     1441 catttccatc tgatttgatt gtaggggccg ggcatgcccg gcctgcagcc ttgccataac
     1501 tgctcgtctg tcttgcacga ccgggccagg catgcctggc ccctacatct ctacaaaaat
     1561 aacgccccca accgtttttg gttgggggtg ctgctatttt caccgattct tcttcataaa
     1621 cgccttgacc gcaattacca gcgcggcgta caccacgccg ccgatggccc agatgaacca
     1681 actgtactgc ggctggccgt ttccctgggg gccgaaggtg taccacagga agatggcgac
     1741 caccaccagc cagtaggcgg cagtcagcgc gtttatgcgg ctcttgatgg ctttgtttgg
     1801 gcgggtgtag tcgccctcct ccaacagctg atccatggcg tcccgcaccg tgcccacccg
     1861 cacaaaggcg tagacgccgc aggccaccag ggccagcacc aggcagatgg acgccgacat
     1921 caaaaacgac gcaccaacca tcatggcggc aaacaccggc accgccgaca aaacgcagag
     1981 caccgtgccg aggatgttgg ccttgtcgta ctgcggcgca aaggcgtcgc ggcgctcctt
     2041 cgcaaggccg gtgacgccgt actcagtctc aatggcctgc ttttgcaaaa attcatacgc
     2101 ccgcaccgac gcgctgcact gcatgaagat agccaccgct gcggcgatga tgaccagcag
     2161 cgcaatgagg ccgatgcccc ccgccaggtc atcccagatg ccgaacaggc cggcctcgct
     2221 caacgccacc agcgccagca gcgggatggg ggagacgaca cagagcgccg ccgccagcgc
     2281 cagcctgggc gcgttggcct tggccttggc aaggtagtcg ttggcctggg ccagcgtcac
     2341 ccggggcagg ggagaggtat cgtcctcggt gtactccggc tcggccgcgt cgtcttttaa
     2401 aaggtagtcg gtcgtcacac cgaacaggca gctcaactgc aagattttgt caatgtcggg
     2461 cacagactgt gcgccctccc acttggatac cgactgccgg gtcacgttca gtttctcggc
     2521 cagttcctct tgggaccaac ccgccttttt gcgcagggtg ataagtttgt ctgcaaagat
     2581 catttgggtg tacctccttc aatttccttg cccttatcct accagaatcc ccggttgcac
     2641 accataaatc ggccccggca atctgtcaac tttgggttgc gtttgcgggc aggcaccagt
     2701 caattttcga tacaccgtta ttttctaaaa aagcatttac tttgccaaac ggtgccgagc
     2761 caaagaaccc gcggtaggcc gaaagcgggc tggggtgggc agattccagc accagcaccg
     2821 ggcggcgggc ggcggccttc tcaaaaatcg ccgcgtattt ctgggcgggc ttgccccaca
     2881 gcacggcggc cagcggggcc gtgcccgcag cagcggcgta ttccagcgcc gcgcgggtga
     2941 acgtctccca cccatggctc gcgtgggcgt tggccgcgcc ctgctctacg gtcagcacgg
     3001 tgttcagcag cagcaccccc tgctgcgccc agggggtcag gtcggtgtgg gcggcacagc
     3061 cggggccgaa ctcgctctcc aattccttaa agatgttgcg caggctcggc ggggccttgc
     3121 agccgtccgg caccgagaag gaaagcccca tagcctgacc cggctcatgg taggggtcct
     3181 gccccaggat gaccacccgc atcgacgatg ccgggcaggc gcggaacgcc gcaaagatat
     3241 tttccggcgc ggggtagacg gtcttttccg ccgcagccgc cgtcacaaag gcgtctaatt
     3301 ccgcaaaata cggctttgcc cgctcctgcg caaaaaacgg ctgccaatcg gtggcggata
     3361 atctgtcgag ttcggcggtt aaaagcatag taaatctcct cggtagcttt gtaggggccg
     3421 ggcatgcccg gcccgtgcgt gtaaaacagg cggtcgttca cgggaaagct gcgggccgca
     3481 catgtgcggc ccctacatag gtgccctcac acatttttca caagtatatc acacccccgc
     3541 aggggttgca atagcggcaa aaatgcggta taatatcatt tatgctgcgg tgtggtgccg
     3601 caaagccaat cataaagggg agaaccaaat ccatgcgttc cattaaaacc aagctgttca
     3661 gccttgccat ggctgtttcc atggtgctgg cgctggctgg ctgcgccatg tccacgccgt
     3721 ccaccgtcgg ctccatcggc gggatcgaga tcccggccgg tatctacctg ctggcccagt
     3781 acaactccta caacaccgcc gccaacgcgg cagagcttgc caccagcgag actgcctccg
     3841 acgtcaaggc cgtgctgaag gccacctgca ccggcaccat cggtgatgag gaagtcaccg
     3901 ccaccggcag tgagtatgtt gccaagctga ccgaccgcgc catttcctac tacgccgagg
     3961 tcgagcgccg ctttaccgag atgaacggcg tgctggatga cgccgctacc gccgaggccg
     4021 ccgacactgc cgacaacctg tggtccacca acggcgacct gtataccgcc aatggcattt
     4081 ccaagacgag tctgcagacc tacctgctca acgcccagaa ggccaaggct ctgctgaaga
     4141 tgacctacgg ccccgacggt acgacgcctg tggccgagga cgagtacacc gacttcgtca
     4201 acaacgactg ctactacatc gaggctgtgc agttcccgct ggtggattac tccacctact
     4261 cgatggccga tgacgaccag aaggccgcca tcatggccac cgccgaaagc tgcatggctg
     4321 aattgaacac gaccgccacc gccgagaccg ccagcaacag cgccctgtac accgccgcca
     4381 tgacctatgt gccgcaggcg atggccgcca tgggcagcag tctggacgcc agccaggccg
     4441 tttactacgc agcttcccag ctgtacacgc cggacgacct gagcagctac ggcagcgacg
     4501 agtacaacaa cctgaccgac ccgctggacg aggccgggct gaaccactgg accaccatca
     4561 acctgggcac gacgattctg gttgcccgta agattgatcc gttcaagacc tacaccgtgg
     4621 acgagttgaa cagtatgtac gacctgttga gcagcatgaa gtccaccgac atccagagcg
     4681 agctgtacgc tgccggtgcc gcactggagc ataacctgaa cagttccgcc ctgaacacat
     4741 acagcgcatc caagattaaa aagaacgttt gagcgtaaaa cgcttggccg ggagcagcat
     4801 tgtttgctcc cggccttttt gttgaggagg atcccctatg acgaaaaaca aatccgtgaa
     4861 cttcctgctg caaatgctgg gctgctttat ctcggcggcg ggcatttaca gctttgcggt
     4921 cgcgtccgaa gtccccgtca ccggcgttgc gggcatctgc gccattctgt accgcctgtt
     4981 cggcatcccc atgggtttga gcaatgtgct catcaacatt cccatcatcc tttgcacgta
     5041 taaattgctc ggcaaatcgt tctttattcg cagtgtctac tgcatggtgc tgtttgcggt
     5101 gttcaccgac tatctgctgc cgctcatgcc ggtctatcag ggcgaccgcc tgctggccac
     5161 aatctgcggc ggcgtggtgg gcggcatcgg cgacgcactg atctacatga acaattccag
     5221 caccggcggc ctggacttca tcaccatggg catcaaggcc aagcacccgc accttccatt
     5281 tggcaacatc acctttgcgg cggcgctggc ggtcattctg ctcaacggcg cagttttcca
     5341 ggatgtggat tctattatct acggtatcat gttcaatttc attgtgtccg ccgtcattaa
     5401 caagatgatg ttcggcttct cgtcgtcgat gctggcaatg attatcaccg acgacggcgc
     5461 ggccgcctgt gctgagattg accgggtagc ggaccgcggc tcgacgattt tgcagggccg
     5521 cggcggctac ggcggccagc cgaaggacgt ggtgctctgt gcctgctcca acaagcaact
     5581 gtacgagatc gagcacgctg tccaagccat cgaccccggc tgtttcatca tcatgctcca
     5641 atccaacgaa gtccacggcg agggcttcag gcagttggaa ttggggaaga acgaaaacaa
     5701 gtcagaataa gtaagtatca tacacacgct gattttgcgc aattctatga agcataggtt
     5761 gccaaaatcg gcgtgtttct ttatgataag ggcaaaacaa tgcaggaggg ctttcttatg
     5821 gatgctacaa aaatcggaac gtttatcgcc gccgaacgcc gcgccaaggg ctggacgcag
     5881 cgccagctgg ccgacaaact tcaactgacg gacaaagcag tcagccgttg ggagacagga
     5941 aagggcctgc cggatgtttc gtttttgctg ccgttggccg ctgctttgga cgtcagcgtt
     6001 ggtgagctgc tggcagggga gagccagccc cagccgcctg cggtacaggc cgccgagaca
     6061 cgcacagcgg aacagcttgc cagctacacg cgggagcttg gcccgcagct gcgccgccgc
     6121 cgtgcctgca cagtgctggc cgggttgctg ctgtttgccg cagcgttttt atctctgcaa
     6181 tttctgcggc tgttgatggg cggggcgggc atccatggtc ttagcgtaac gtctctgcta
     6241 ctgcttgtcg ttctgccgtt tgtttttgcg acattcgcct tttggctgct gcacctgctc
     6301 tgcacggatg gcctggcgga aacgcgggct tttcgccgtg catctgctat ggccgtggct
     6361 gggctgtggc tgttcgattt gtactttgcc acagcatttt ccggatgtgc agcgttcccg
     6421 gtgctggatt tctggcgcgc cccatcgtgg caggcaaatg taaatctggt gccgtttcgg
     6481 caaatcatgc agtacctgct ctgtctggtg ccggggcagg ggtacttgaa cggcggcggg
     6541 tatgcgttcc gcgggcattt tgccttctgg atgaactttt tcgtgttttc cgtgccggtt
     6601 ctgctgctgc cgcgc
//
LOCUS       DBMDSQ010000088         6438 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_3072_length_6438_cov_1.829888, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000088 DBMDSQ010000000
VERSION     DBMDSQ010000088.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 6438)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6438)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6438
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3072_length_6438_cov_1.829888"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1484)
                     /gene="aspS"
                     /locus_tag="ACZPAX_04575"
     CDS             complement(<1..1484)
                     /gene="aspS"
                     /locus_tag="ACZPAX_04575"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HIR1113206.1"
                     /translation="METLGSLRRTNYCGEVSLAMAGQEMTVCGSIARARDKGGIIFAD
                     LRDTTGILQLVFDEDTPKDVFAKAESLKSEYVIICRGKLRERAAKTDKISTGDVELYV
                     TELRILSEAQTTPFELRDEINVNDDLRLRYRYLDLRRPSMHEPIVLRSKIMQIIRNYF
                     CENHFTEIETPTLIKSTPEGARDYLVPSRVQPGHFYALPQSPQLYKQILMLSGFDRYF
                     QLAHCYRDEDLRADRQPEFTQVDEEMSFVSEDDIMTLNEGLIKRLWKEMLNVDVETPF
                     YRMPWDEAMGRFGSDKPDTRFGLEIQDVTEVFKGTEFKPFAAVLEAGGTIRAINAKGL
                     ADKLSRKNIDKLGEVAKTYGAKGLAYSRLTADGTSSSFEKFLTDAEKAALYAALNAET
                     GDVLLLVSDTDWVKACTALGQVRLDIARKHGLIDPDKFNFLWVVDFPLFEYSEQEGRW
                     MAMHHPFTLPKAEDLDKVESDPGACHAVAYDIVLNGVEMGGGSMR"
     gene            1671..1746
                     /locus_tag="ACZPAX_04580"
     tRNA            1671..1746
                     /locus_tag="ACZPAX_04580"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1704..1706,aa:Lys,seq:ttt)
     gene            1909..2160
                     /locus_tag="ACZPAX_04585"
     CDS             1909..2160
                     /locus_tag="ACZPAX_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HIR1113207.1"
                     /translation="MPISEPTAVFSIRDDRDMEIKQAVLQVYRALEEKGYNPINQLVG
                     YILSEDPTYITTYKNARAIIRRIDRDDLLQALVRDFVKH"
     gene            complement(2250..3512)
                     /locus_tag="ACZPAX_04590"
     CDS             complement(2250..3512)
                     /locus_tag="ACZPAX_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015565107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine gamma-lyase family protein"
                     /protein_id="HIR1113208.1"
                     /translation="MIEQFYDLRPEVLALDRKALDLCRAQFAHVEEIRDYNQLKMLRA
                     FTDCGVEARHFWGSSGYGVWDDSRNKLEEVFARCMGAEAALVRPQFMSGTHTLTVALF
                     GLLRTGDTLLAATGRPYDTLEGVIGIGNAGKGCGTLREFGVNYDECPLLPDFTPDYDL
                     IAEKAKTATVVHIQRSRGYTQRNAFDLATIQKVADTARRANPNAVIFVDNCYGEFTQT
                     AEPVSFGADIMAGSFIKNPGGGITPTGGYIAGRRDLVEKCSHRFTAPGIGGDLGCTQD
                     SLRDTFLGFYYAPGVVCEALKTAIYAQCLLELAGVNPVPRYTADHNDIVTCFDSGSAA
                     ALTGFCAGIQHNSPVDSFASPEPADEPGYTDKVVMASGSFTEGSTIEISCDGPLRAPY
                     TCYLQGGVNFTAGRAAVLNAVQNAFFAE"
     gene            complement(3509..4294)
                     /locus_tag="ACZPAX_04595"
     CDS             complement(3509..4294)
                     /locus_tag="ACZPAX_04595"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR1113209.1"
                     /translation="MIQSASTSALRAEYLRRLADKPYFAANLGTHLNLFGQHPASGWA
                     FYLLPGSAVLELRGGSAVLCGELPGGDAGGDAREELRGFLQFLHADTLRSEHPLALDG
                     WRPAAPLALWELPRGRTLPLPPAPPAELMLDKAPSMLPISRLVFPDSDAEADEFYSTA
                     CTALAHGVGNCRALLHGRKPVCTVGCYEASPAECYMAAGVTDPAWRGRGLARWLIVSL
                     ANELAADRTVRFACFPELCDFYAQLGFLQIGKIQHYSTDWNTQ"
     gene            complement(4291..5688)
                     /gene="murD"
                     /locus_tag="ACZPAX_04600"
     CDS             complement(4291..5688)
                     /gene="murD"
                     /locus_tag="ACZPAX_04600"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HIR1113210.1"
                     /translation="MLPIEQLQELIKGKKVAFIGAGVSHKRCIAQFVELGAQVTLCDQ
                     KKSLDDFGDYADTLRRLNVNLSLGEHYTDGFAGQDIIMRTPGYEYYKPELQAAKAAGA
                     LVTSEVELFFAFCPCEIVAVTGSDGKTTTTTLISKMFEAAGRRVFLGGNIGAALLPQL
                     SDVTPDAVAVVELSSFQLISMRRSPKVAVVTNVTPNHLDHHKDMQEYIDAKRNILLWQ
                     TPPCRAVLGYENDVSRSMQSDCKGEQVWFTRLHETDNGAFLRESDDTLCYAENGVVTP
                     ILPRKDIKLRGLHNVENLLAACAAVWGRVPIEAMRQVGSTFTGVEHRIEPVRTLDGVT
                     YYNDSIASSPTRTIAGLRSFDQKIILIAGGYDKKIPYEPLAPEILAHVKTLVLMGATG
                     PRIEKAVRECPGFAESSLTILHADNMQHAVELARSAAKSGDVVSLSPASASFDLYPNF
                     EVRGKDYKNIVNALK"
     gene            complement(5797..>6438)
                     /locus_tag="ACZPAX_04605"
     CDS             complement(5797..>6438)
                     /locus_tag="ACZPAX_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009987480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HIR1113211.1"
                     /translation="AVPTMSFDNVMPTYQVLKCLLHHVPDVSFHKDNFMIVSPDEGAM
                     NRNMYYSSVLGCNLGMFYKRRDYSRIVNGRNPIVAHEYLGESVQGKDVFIADDIIASG
                     ESMLDIAYELKMRGARNIFTCCTFPLFTAGLEKFDKAYNDGIIKAVLGTNLTYRKPEL
                     LEREWYYDVDVSKYTAYFIAAINHDKSVSSIIDPMTKIRALLDKHGIPMGGEQ"
BASE COUNT         1243 a         1871 c         2028 g         1296 t
ORIGIN      
        1 cgcatggagc cgccgcccat ctcgacaccg ttcagcacga tatcgtaggc cacagcgtgg
       61 caggcaccgg ggtcgctctc gaccttgtcc aggtcttcgg ccttgggcag ggtgaacgga
      121 tggtgcatgg ccatccagcg gccctcctgc tcggagtact cgaacagcgg gaagtcaacg
      181 acccacagga agttgaattt gtcggggtcg atcaggccgt gcttgcgggc aatgtccagg
      241 cggacctggc ccagcgcggt gcatgccttg acccaatcgg tatcgctgac cagcagcaac
      301 acgtcgccgg tctcggcgtt cagtgcggca tacagcgcag ctttctcggc gtcggtcaga
      361 aatttctcaa agctggagga agtaccgtcg gcagtcaggc ggctgtacgc caggcccttg
      421 gcgccgtagg tcttggcgac ctcgcccagc ttatcgatgt tcttgcggga cagcttatcg
      481 gccagacctt tggcgttgat ggcgcggatg gtgccgcctg cttccagcac ggcggcgaag
      541 ggcttgaact cggtgccctt gaacacctcc gtcacgtcct ggatttccag accaaagcgg
      601 gtgtcgggct tatcggagcc gaagcggccc atagcctcat cccagggcat acggtagaac
      661 ggcgtctcga cgtcgacgtt cagcatttcc ttccacaggc gcttgatcag gccctcgttc
      721 agggtcatga tatcgtcctc ggagacaaag ctcatctcct cgtccacctg ggtgaactcg
      781 ggctgacggt cggcgcgcag atcctcgtcg cggtagcagt gagccagctg gaaatagcgg
      841 tcaaagccgg acagcatcag aatctgctta tagagctgcg ggctctgggg cagggcgtag
      901 aagtggcccg gctgcacacg gctaggcacc agatagtcac gcgcaccctc gggcgtggac
      961 ttgatgaggg ttggcgtttc gatctcggtg aagtggttct cgcagaaata gttgcggatg
     1021 atctgcatga tcttgctgcg cagcacgatc ggctcgtgca tggacgggcg gcgcaggtca
     1081 aggtaacggt agcgcaggcg caggtcgtcg ttgacgttga tctcgtcgcg cagctcaaac
     1141 ggcgtggtct gggcctcgct caggatgcgc agctcggtaa cgtagagctc cacgtcaccg
     1201 gttgaaatct tgtcggtctt ggcggcgcgc tcgcgcagct tgccgcggca gatgatgacg
     1261 tactcgcttt tcaggctctc ggccttggcg aaaacgtcct tgggggtatc ctcatcgaac
     1321 accagctgca aaatgccagt ggtatcgcgc aggtcggcaa agatgatgcc gcccttatcg
     1381 cgggcgcggg cgatggagcc gcagacggtc atttcctgac ccgccatggc aaggctgact
     1441 tccccgcagt aattggtacg gcgcaggctg cccaaagttt ccatatttcc aaatcccctt
     1501 tacggttttt tctactctaa aaacctatta taccgcgctg tcctgcgttt ggcaaggggc
     1561 gcggggcgaa aggcggagaa aatccgcgta aaatgttttc aaaagtgtat tgacaagcgg
     1621 ggccggactt gctataatag tagggctgtg agacccacag tgcaatagat gtgcccgtag
     1681 ctcagttggt agagcatctg acttttaatc agagggtcca ggattcgagt tcctgcgggc
     1741 gcaccaaaaa ccgcttgaag ccgcaaggtt tcaagcggtt ttttgcatta tacaacagca
     1801 aggacgaaat ttgcatttct gtataaaatt ttatggtttt cgtcttgcaa atttggcgcg
     1861 tgttgagtat aataaggcta atgatatctt gtaaagggga gaattgaaat gccgatctct
     1921 gaaccaaccg ccgtattttc catccgtgat gaccgtgata tggagatcaa gcaggcggtg
     1981 ctgcaagtgt accgcgcgct ggaggagaag ggctacaacc ccatcaatca gctcgtcggc
     2041 tacatcctgt cggaggatcc gacctacatc acgacctaca agaacgcccg cgccatcatc
     2101 cgccgcattg accgcgacga cctgctgcag gcgctggttc gcgattttgt gaagcattaa
     2161 aggtttggca aaaaattccc gcatatccga tttggatatg cgggaatttt tgtagctaaa
     2221 gccttctccc tcgggagaag ccttttttct tattccgcaa aaaacgcgtt ctgcacggcg
     2281 ttcaggacgg cggcacggcc ggcggtaaag ttgacgccgc cctgcaggta gcaggtgtag
     2341 ggggcgcgca gcggaccgtc gcagctgatt tcgatcgtac tgccctcggt aaagctgccg
     2401 ctggccatga cgaccttgtc cgtgtagccc ggctcgtcgg cgggttcggg gctggcgaag
     2461 ctgtccaccg ggctgttgtg ctggatgccc gcgcagaagc cagtcaacgc ggcggcactg
     2521 cccgagtcaa agcaggtcac aatatcgttg tggtcagccg tatagcgcgg taccgggttg
     2581 acgcccgcca gttccagcag gcactgggca taaatcgcgg ttttcagcgc ctcgcagaca
     2641 acaccgggcg cgtagtaaaa gcccaggaag gtatcccgca ggctgtcctg ggtgcagccc
     2701 aggtcgccgc cgatgccggg agccgtgaag cggtggctgc acttttccac caggtcgcgg
     2761 cggcccgcaa tgtagccgcc ggtgggggtg atgccgccgc cggggttctt gatgaagctg
     2821 cctgccatga tgtccgcgcc gaaggatacc ggctcggcgg tctgggtgaa ctcgccgtag
     2881 cagttgtcca caaagatcac agcgttgggg ttggccctgc gggcggtgtc ggccaccttt
     2941 tgaatcgtcg ccaggtcaaa ggcgttgcgc tgggtgtagc cgcggctgcg ctgaatgtgc
     3001 accactgtcg cggtcttggc cttctcggca atcaggtcat agtcgggcgt aaagtccggc
     3061 agcagcgggc actcgtcgta gtttacaccg aactcccgca gcgtaccgca gcccttgccc
     3121 gcattaccga tgccgatgac gccttccagc gtgtcatagg ggcggccggt ggcggccagc
     3181 agggtgtcgc cggtgcgcag caggccgaac agcgccacgg tcagcgtgtg ggtgccgctc
     3241 ataaactgcg ggcgcacgag ggcggcctcg gcccccatgc agcgggcaaa gacctcttcc
     3301 agcttgttgc ggctgtcgtc ccacacgcca tagccgctgg acccccagaa atgccgcgcc
     3361 tcgacgccgc agtcggtgaa ggcgcgcagc attttcagct ggttgtagtc tcgaatctcc
     3421 tccacatggg caaactgcgc acggcagagg tcaagggctt tgcggtccag cgccagcact
     3481 tcggggcgca ggtcataaaa ttgttcgatc attgagtatt ccaatctgta ctataatgtt
     3541 gaattttgcc gatctgtaaa aatcctagct gggcataaaa atcgcacagt tccgggaaac
     3601 aggcgaaccg cacagtgcgg tcggcggcca gttcgttggc aaggctcacg atgagccacc
     3661 gggccagtcc cctgccccgc caggcggggt cggtaacacc cgcggccatg tagcattcgg
     3721 caggggatgc ctcatagcag cccacggtgc agacaggctt gcggccgtgc agcagggcac
     3781 ggcagttacc cacaccatgg gccagcgccg tgcaggcggt ggagtagaac tcgtcggcct
     3841 cggcgtcact gtcaggaaac accaggcggc tgatgggcag catcgacggc gctttgtcca
     3901 gcatcagttc tgccgggggc gctggcggca agggcagcgt gcgcccccgg ggcagttccc
     3961 acagggccag cggcgcggcg ggtcgccagc cgtcaagggc cagcggatgc tcgctacgca
     4021 gcgtatcggc gtggaggaat tgcaagaaac cccgcagttc ctcccgggcg tcgccacccg
     4081 cgtctccgcc gggcaattcg ccgcagagca cagccgagcc gccccgcagt tccagcacag
     4141 ccgagccggg cagcaggtag aacgcccacc cgctggccgg gtgctggcca aataaattca
     4201 gatgcgtgcc gaggttggcc gcaaagtagg gcttatcggc aaggcggcgg agatattctg
     4261 cgcggagggc ggaggtagag gcggattgaa tcatttcagt gcattcacga tattcttgta
     4321 atccttcccg cgcacctcaa agttagggta caaatcaaag gacgcggagg cgggcgacag
     4381 gctgaccacg tcgccggact tcgcagcgga acgcgccagt tcaacagcgt gctgcatatt
     4441 gtcggcgtgc aggatcgtca ggctgctctc ggcaaagccc gggcactcgc ggacagcttt
     4501 ctcgatgcgg gggccggtcg cgcccatcag caccagcgtc ttgacgtggg ccagaatctc
     4561 cggggccagc ggctcatagg ggatcttctt gtcatagccg cccgcgatca gaataatttt
     4621 ctggtcaaag ctgcgcagac ccgcgatcgt gcgcgtcggg ctggacgcga tggagtcatt
     4681 gtagtaggtc acaccgtcca gcgtgcgcac cggctcaatg cggtgttcga cgccggtgaa
     4741 ggtcgagccg acctgacgca tggcctcgat gggcacacgg ccccagacgg cggcacaggc
     4801 ggccagcagg ttttccacat tgtgcaggcc gcgcagttta atatccttgc ggggcagaat
     4861 cggcgttacc acgccgttct cggcatagca gagcgtgtcg tcgctttccc gcaggaacgc
     4921 accgttgtcc gtctcgtgca ggcgggtgaa ccagacctgc tcgcccttgc agtcgctctg
     4981 catactgcgg gaaacgtcgt tctcatagcc cagcacggcg cggcagggcg gggtctgcca
     5041 cagcagaatg ttccgcttgg cgtcgatata ctcctgcata tccttgtgat ggtccaggtg
     5101 gttgggggtg acgttggtca cgaccgccac cttcgggctg cggcgcatac tgatgagctg
     5161 gaagctggac agctcgacga ccgccaccgc gtcgggggtg acgtcggaca gctggggcag
     5221 cagtgccgca ccgatgttgc cgcccaggaa caccctgcgg cctgcggctt caaacatctt
     5281 ggagatcagc gtcgtggtgg tggtcttgcc gtcagagccg gtcacggcca cgatttcgca
     5341 ggggcaaaac gcaaagaaaa gctccacctc gctggtgacc agtgcaccgg cagccttggc
     5401 ggcctgcagc tcgggcttgt agtactcgta gcccggcgtg cgcatgatga tatcctgtcc
     5461 ggcaaagccg tcggtgtaat gctcgcccag gctcaaattc acattcaggc ggcgcagcgt
     5521 gtcggcgtag tcgccgaagt cgtccaggct ctttttctgg tcgcagagcg tgacctgcgc
     5581 gcccagttcc acaaactgcg caatgcagcg cttgtggctg acaccagcac cgataaaggc
     5641 aacctttttg cccttgataa gttcttgcag ctgttcgatc ggcagcataa taatctctcc
     5701 cctgcttata atctaaccac attatatacg aaatgccgta ttcctgcaac aaaattcggc
     5761 ccgccgaagc gagccgaggt gggatttatt ttgcgcttac tgctcgccgc ccatggggat
     5821 gccgtgcttg tccagcaggg cgcggatctt cgtcatcggg tcgatgatgg aagaaacgct
     5881 cttgtcatgg ttgatggcgg cgataaagta ggcggtgtac ttggagacgt ccacgtcata
     5941 gtaccactcg cgctccagca gctccggctt gcggtaggtc aggttcgtgc ccagcacagc
     6001 cttgatgatg ccgtcgttgt aggccttgtc gaacttctcg agaccggcgg tgaacagcgg
     6061 gaaggtgcag caagtgaaga tgttgcgggc gccgcgcatc ttcagctcat aggcgatatc
     6121 cagcatggac tcaccggagg cgatgatgtc atcggcgatg aacacgtcct taccctgcac
     6181 gctctcgccc aggtattcat gggcaacgat ggggttgcgg ccattgacga tgcggctgta
     6241 atcgcggcgc ttgtagaaca tgcccaggtt gcagcccagc acgctggaat agtacatatt
     6301 gcggttcatc gcgccctcgt ccggggagac gatcatgaag ttgtccttgt ggaaggagac
     6361 gtccggcacg tgatgcagca ggcacttgag cacctggtag gtgggcatga cgttgtcgaa
     6421 gctcatggtg ggcacggc
//
LOCUS       DBMDSQ010000089         6371 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_3107_length_6371_cov_1.979585, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000089 DBMDSQ010000000
VERSION     DBMDSQ010000089.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 6371)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6371)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6371
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3107_length_6371_cov_1.979585"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /locus_tag="ACZPAX_04610"
     CDS             complement(<1..288)
                     /locus_tag="ACZPAX_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015564648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein uS4 family protein"
                     /protein_id="HIR1113212.1"
                     /translation="MAKNTQPIAKRCRALGISPAVMGYGKKTTNRNPGGQMRKKKSEY
                     ATQLNEKQKVKFVYGILESQFHTYYEKASRMPGQTGVNLLVLVERRLDNVVY"
     gene            complement(309..713)
                     /gene="rpsK"
                     /locus_tag="ACZPAX_04615"
     CDS             complement(309..713)
                     /gene="rpsK"
                     /locus_tag="ACZPAX_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HIR1113213.1"
                     /translation="MAANKATAAKTRTKKKERKNISAGAAHIQSTFNNTIVTITDTQG
                     NTVSWCSTGALNFRGSRKSTPYAAQSAAEVAAKAAIEHGMKTVEVYVKGPGSGRESAI
                     RALQATGLEVTMIRDVTPIPHNGCRPPKRRRV"
     gene            complement(728..1099)
                     /gene="rpsM"
                     /locus_tag="ACZPAX_04620"
     CDS             complement(728..1099)
                     /gene="rpsM"
                     /locus_tag="ACZPAX_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015390359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HIR1113214.1"
                     /translation="MARIAGVDLPREKRIEVGLTYVYGIGQSTADEILAGTGINPDTR
                     VKDLTADEEAKIRDYIDKNNIMVEGDLRRNVALDIKRLTEIQCYRGVRHRKGLPCRGQ
                     RTKTNARTCKGPKRTVANKKK"
     gene            complement(1213..1326)
                     /gene="rpmJ"
                     /locus_tag="ACZPAX_04625"
     CDS             complement(1213..1326)
                     /gene="rpmJ"
                     /locus_tag="ACZPAX_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HIR1113215.1"
                     /translation="MKVKPSVKPICEKCKVIKRKGRVMIICANPKHKQRQG"
     gene            complement(1360..1596)
                     /gene="infA"
                     /locus_tag="ACZPAX_04630"
     CDS             complement(1360..1596)
                     /gene="infA"
                     /locus_tag="ACZPAX_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009065662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HIR1113216.1"
                     /translation="MSKEDVIEVEGIVREAMPNTVFKVELLNKEGKPNGHVVMAHISG
                     KLRTNFIRILPGDKVTMEMSPYDLTKARITWRSK"
     gene            complement(1623..1871)
                     /locus_tag="ACZPAX_04635"
     CDS             complement(1623..1871)
                     /locus_tag="ACZPAX_04635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="KOW domain-containing RNA-binding protein"
                     /protein_id="HIR1113217.1"
                     /translation="MDFVKGQLVRSKAGRDKTRTLAVLAVDGSMLLIADGDLHKLDNP
                     KRKKIQHVAPTTTVLENELLKSDQQLRDAIRAYDAAQN"
     gene            complement(1859..2632)
                     /gene="map"
                     /locus_tag="ACZPAX_04640"
     CDS             complement(1859..2632)
                     /gene="map"
                     /locus_tag="ACZPAX_04640"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HIR1113218.1"
                     /translation="MIQIKNAAELQKMRKACAISAAALKAGGEAIEPGITTAEIDKII
                     YDFIVRHGARPNFLHLYGFPATACISVNDTVIHGIPSKQQQIRPGDIVSIDTGCKIDG
                     FNGDNACTYGCGKLDLEAQRLLDVTKESLHRGIEMARGGNRVGDIGHAVQTYVEENGF
                     SVVRTFVGHGVGKELHEDPEVPNFGNAGRGPRLVPGMCIAIEPMVCQYKYAVKTLKDG
                     WTVKTCDGGLAAHFEHTMAITTAGAEVLTHGWEEPGWTL"
     gene            complement(2639..3271)
                     /locus_tag="ACZPAX_04645"
     CDS             complement(2639..3271)
                     /locus_tag="ACZPAX_04645"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015564646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HIR1113219.1"
                     /translation="MKLILLGAPGAGKGTQAEILCDKLGIPTISTGNILRAAVKEGTP
                     MGKKAKSFMDAGALVPDDVIVGIVKERLAEPDCEKGFILDGMPRTIAQGEALEQMGVD
                     IDKVVNLIVPDEAITRRMSGRRVCAKCGASYHIVNKPSAKKGVCDRCGGELAIRKDDE
                     PATVLDRLKAYHEQTEPLVEFYRQRGKLVEVPDQGSIEATTAFLLKLLEA"
     gene            complement(3289..4593)
                     /gene="secY"
                     /locus_tag="ACZPAX_04650"
     CDS             complement(3289..4593)
                     /gene="secY"
                     /locus_tag="ACZPAX_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HIR1113220.1"
                     /translation="MLETFRNAWKIEDLRKRLLFTLLILVLFRLGCAIPVPYISAGAL
                     TSMFAGGTGDMLEYLNMMSGGALSECTLFALGVQPAINASIIMQLLAVAIPYLENLTK
                     EGEEGQRKMRRITNYVGAGIGLMLSIGYYFIIRNMGALSYTEGFAGIFSAVVIVLAFT
                     AGSQLCAWMGNQIDSKGIGNGISLMIFAGIVARWSSIYTATTNILARAQNGEPFFYIM
                     LPLLVVLALVAVVFVVILTNAERRIPVQYAKRVMGRKMYGGQASYIPIKVNMTGVMPI
                     IFASTLCSLPGLIFRFINLDAASHPALYAFFSVFNYNSVLYLIVYVLLIVAFNYFYVA
                     IQYNPVDIANQLRKNNGTIPGIRPGKPTSDFITKTLSKITLIGAIFLAIVAGFPIILG
                     NITGTSIQLGGTTLLIVVGVALETGRSLEGYMTARYHKGFLE"
     gene            complement(4595..5032)
                     /gene="rplO"
                     /locus_tag="ACZPAX_04655"
     CDS             complement(4595..5032)
                     /gene="rplO"
                     /locus_tag="ACZPAX_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015564645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HIR1113221.1"
                     /translation="MKLHELKASAGARKAVTRKGRGAGSGNGKTAGFGHKGQKARSGV
                     KKAGFEGGQMPLQRRLPKRGFNNIFATKYVTIKVSDLEKFEAGSTVDTEALLKAGIIS
                     KTLDGVKVLGNGELTKAVNVKVAAYTASAKEKIEKAGGKAEVM"
     gene            complement(5050..5229)
                     /gene="rpmD"
                     /locus_tag="ACZPAX_04660"
     CDS             complement(5050..5229)
                     /gene="rpmD"
                     /locus_tag="ACZPAX_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HIR1113222.1"
                     /translation="MEKVTIRLAKSLIGRGKEQIAVAHSLGLNRPGEVTEQPDNAATQ
                     GKIAKIAHLVVVTKG"
     gene            complement(5243..5746)
                     /gene="rpsE"
                     /locus_tag="ACZPAX_04665"
     CDS             complement(5243..5746)
                     /gene="rpsE"
                     /locus_tag="ACZPAX_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005937997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HIR1113223.1"
                     /translation="MAMQRRQEDDGMITKVVSINRVSKTVKGGRVMKFAALIVVGDGK
                     GNIGYGVGKSGEVPEAIRKGEGAAKKNMRKVCLKGTTIPHEIVGEFGAGRVLMRPAAP
                     GTGVLAGGPVRAVLECAGIKDIRAKSLRSNNPINVTAATFAGLCGLTDAESVAAKRGK
                     TVAEILG"
     gene            complement(5763..6122)
                     /gene="rplR"
                     /locus_tag="ACZPAX_04670"
     CDS             complement(5763..6122)
                     /gene="rplR"
                     /locus_tag="ACZPAX_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005938000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HIR1113224.1"
                     /translation="MVNKADKNVARLRRHRRVRGKISGTAARPRLDVFRSAKHIYAQV
                     IDDEQGVTLASASSMDKDFNAYGGNIDAAKKVGEAIAKKCLEKGITEVVYDRGGFVYH
                     GRVQALAEGAREAGLKL"
     gene            complement(6141..>6371)
                     /locus_tag="ACZPAX_04675"
     CDS             complement(6141..>6371)
                     /locus_tag="ACZPAX_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015564642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HIR1113225.1"
                     /translation="LTLGFSHPVEFEDTDTIKIELKDALHFSITGIDKQEVGQFAAEV
                     RGVRPPEPYKGKGIRYVGEYVIRKEGKAGKGK"
BASE COUNT         1244 a         1835 c         1865 g         1427 t
ORIGIN      
        1 gtagacgacg ttgtcgagac ggcgctcgac caggaccagc aggttgacac cggtctgacc
       61 gggcatgcgg gaggccttct cgtagtaggt atggaactgg ctctccagga taccgtacac
      121 gaacttgacc ttctgcttct cgttgagctg ggtggcgtac tcgctcttct tctttctcat
      181 ctggccgccg gggttacggt tggtggtttt cttaccatag cccataacag caggagaaat
      241 gccgagagca cggcagcgct tagcgatagg ttgggtattc ttagccatat ctcatgttct
      301 cctttcaatc aaacgcggcg gcgtttgggc gggcggcagc cattgtgggg gatgggggtc
      361 acgtcacgga tcatggtgac ctccaggccg gttgcctgca gggcgcggat agcgctctca
      421 cggccggaac cggggccctt aacgtagacc tcaacggtct tcatgccatg ctcgatggcg
      481 gccttggcag cgacctcagc agcgctctgc gcagcgtaag gagtgctctt acgggagcca
      541 cggaagttca acgcaccagt gctgcaccag gaaacggtgt tgccctgggt gtcggtgatg
      601 gtcacgatgg tgttgttaaa ggtgctctgg atgtgagcag caccggcgct gatattcttg
      661 cgctcttttt tcttagtgcg ggtttttgcc gcagtagctt tgttagcagc catgtctcag
      721 ttcctcctta cttctttttg ttagcgacag tgcgcttggg acctttgcag gtacgcgcat
      781 tggtcttagt gcgctggccg cggcagggaa gacccttgcg atggcgcaca ccacggtagc
      841 actggatctc ggtcagacgc ttgatgtcaa gcgcgacgtt acggcgcagg tcgccctcta
      901 ccatgatgtt gtttttgtcg atgtagtcgc ggatcttggc ctcttcatca gcagtcagat
      961 ccttgacgcg ggtatcaggg ttaattccgg taccggcgag aatctcgtcg gcagtggact
     1021 ggccaatgcc gtaaacataa gtcagaccaa cctcgatccg cttctcgcga ggcagatcaa
     1081 caccagcaat acgtgccata aagcacctcc attagtaaca tcccatgtcc tggtcggggc
     1141 ctgcgccggg aatctacgca cttggccccc aagcgttttg ggtacggttt ccgcggaatg
     1201 ctccgcggac ctttagccct gacgctgctt atgcttcgga ttggcgcaga tgatcatcac
     1261 gcggcctttg cgcttgatga ccttgcactt ctcgcaaatg ggtttcacgg aaggttttac
     1321 cttcatgatg ttaacctcct ttataacaca aggcgcttct tatttggaac gccaggtgat
     1381 ccgggctttg gtcaggtcgt agggagacat ttccatcgtg accttgtcgc cgggcaggat
     1441 gcggatgaag ttggtccgca gcttgccgga aatgtgagcc ataaccacat ggccgttggg
     1501 cttaccctct ttgttgagga gttcaacctt gaacacagtg ttgggcatgg cctcccgaac
     1561 gataccttct acttcgatga catcttcttt agacaagctg attgcctcct tgaagggttc
     1621 cgtcagttct gcgccgcgtc ataggcgcgg atggcatccc tgagttgttg atcgcttttc
     1681 agaagctcgt tctcaagaac ggtggtcgta ggtgcaacgt gctgtatctt tttgcgtttg
     1741 gggttgtcca gcttgtgcag gtccccatcc gcaatcagaa gcatcgatcc atccacagcc
     1801 agaacagcca gcgtgcgggt cttatcccgc ccggcctttg agcggaccaa ttggcctttt
     1861 acaaagtcca tccgggttcc tcccagccgt gggtcaggac ctcggcaccg gccgtggtga
     1921 tggccatggt gtgctcaaaa tgagccgcca ggccgccgtc acacgttttg accgtccagc
     1981 cgtccttcag cgtctttacc gcgtacttgt actggcagac catcggctca atggcaatgc
     2041 acatacccgg caccaagcgc ggaccgcgcc cggcgttgcc gaagttcggg acttcgggat
     2101 cctcgtgcag ctccttgccg acgccatggc cgacaaaggt gcggacgacc gagaagccgt
     2161 tctcctcaac ataggtctgt actgcgtggc cgatatcccc tacccggttg ccgccgcgcg
     2221 ccatctcaat gccgcgatgc aggctctctt tggtaacatc caacagccgc tgggcttcca
     2281 ggtcgagctt gccgcaccca taggtgcagg cattatcgcc gttgaagccg tctatcttgc
     2341 agccggtatc gatcgaaacg atgtcaccgg ggcggatctg ctgctgtttt gaggggatac
     2401 cgtgaatgac ggtgtcattc acgctgatgc acgccgtggc cggaaagccg tacaggtgca
     2461 ggaagttggg tcttgcaccg tggcgcacga taaagtcgta gatgattttg tcgatctcag
     2521 cggtagtgat accgggttcg atcgcttcgc cacctgcttt caaggcagct gcgctgatag
     2581 cgcaagcctt gcgcattttt tgcagctcgg cagcattttt gatttggatc atggatgatt
     2641 aagcctccaa aagcttgaga aggaacgccg tagtggcctc aatggagccc tggtcgggca
     2701 cctccacgag cttgccgcgc tggcgataga actcgaccag cggttcagtc tgctcatggt
     2761 aagccttcaa gcggtccagt acggtggcag gctcgtcatc cttgcggatg gccagctcac
     2821 cgccgcagcg gtcacaaacg ccctttttgg cgctgggttt gtttacgatg tggtagctgg
     2881 cgccgcactt tgcgcagacg cggcggccgg acatacggcg ggtgatggcc tcgtcgggca
     2941 cgatcaggtt cacgacctta tcgatgtcca cgcccatctg ctccagggcc tcgccctgag
     3001 cgatggtgcg cggcataccg tcgaggataa agcccttctc gcagtcaggc tctgccaggc
     3061 gctctttcac gatgccgacg ataacatcgt caggcaccag agcaccggca tccataaaac
     3121 tttttgcctt tttgcccatc ggcgtgcctt ccttgacggc ggcgcgcaga atgttgccgg
     3181 tggaaatggt cgggatgccc agcttatcgc agaggatctc tgcctgggta cccttgccag
     3241 cgccgggagc gcccaaaagg atcagtttca tcgcacattc cctttctctt attccaagaa
     3301 ccctttgtgg taacgggcgg tcatatagcc ttccagagaa cggccggtct caagcgccac
     3361 acccacgacg atcagcagcg tagtaccacc cagctggatg gaagtgccgg tgatgttgcc
     3421 gaggatgatg gggaagccag caacgatggc caggaagatc gcgccgatca gagtgatctt
     3481 ggacagggtc ttcgtgatga aatcgctggt gggcttgccc ggacggatgc cgggaatggt
     3541 accgttgttt ttgcgaagct ggttggcaat gtccacgggg ttgtactgga tcgcaacgta
     3601 gaagtagttg aacgcgacga tcagcagcac gtaaacgatg aggtaaagaa cgctgttgta
     3661 gttaaacacg ctgaagaaag cgtacagcgc cggatgcgaa gctgcatcca gattgatgaa
     3721 ccggaagatc agaccgggca ggctgcacag cgtcgaagca aagatgatgg gcataacgcc
     3781 ggtcatgttc actttgatgg ggatgtagct ggcctgacca ccatacatct tgcggcccat
     3841 aacgcgcttg gcgtactgga ccgggatgcg gcgctctgcg ttcgtcagga tgacgacgaa
     3901 gaccacagcg accagagcca ggacaaccag cagaggcagc atgatgtaga agaacggctc
     3961 accgttctgg gcgcgggcca gaatgttggt cgtagcagtg taaatgctgg accagcgggc
     4021 cacgatgcct gcgaagatca tcagcgagat accgttgccg atacccttgc tgtcgatctg
     4081 gttgcccatc caagcgcaga gctgagagcc tgccgtgaag gccaacacga tgacgaccgc
     4141 gctgaagatg cccgcaaagc cctcggtgta agacagagca cccatattgc ggatgatgaa
     4201 gtagtagccg atggacagca tcagaccaat gcccgcacca acatagttgg tgatgcggcg
     4261 catcttgcgc tgaccttcct cgccttcctt ggtgaggttt tccagatacg gaatggctac
     4321 cgccagcagc tgcataatga tggaggcgtt gatggcgggc tgcacaccca gggcgaacaa
     4381 cgtgcattcg ctcagagcgc cgccgctcat catattcaga tactccagca tatcgccggt
     4441 gccgccggca aacatcgagg tcagtgcgcc tgcgctgatg tacggcacag gaatcgcaca
     4501 gccaagacgg aacaggacga ggatcagaag ggtgaacaga agacgcttgc gcagatcttc
     4561 aatcttccat gcgttacgga aggtttccag caccttacat cacctcagcc ttcccgcccg
     4621 ctttctcgat tttttccttg gcggaggctg tataagcagc gaccttgaca ttgacagcct
     4681 tggtcagctc gccgttgccc agcaccttga cgccgtccag cgtcttggag atgatgccgg
     4741 ccttcagcag ggcctcggta tcaacggtag aaccggcctc gaacttctcg aggtcagaca
     4801 ccttgatggt aacatacttg gttgcgaaaa tgttgttgaa accgcgcttc ggcagacgac
     4861 gctgcagagg catctggccg ccttcgaagc cagccttctt gacgccgctg cgagccttct
     4921 ggcccttgtg gccgaagcca gcggttttgc cgttgccgga accagcaccg cggcccttgc
     4981 gggtcacggc cttgcgggca ccagcggaag ctttcagttc gtgaagtttc atgcttgcac
     5041 ctccttgcat tagcccttgg tcacgacgac caggtgggcg atcttggcga tcttgccctg
     5101 ggtagccgcg ttgtcgggct gctcggtaac ctcaccgggg cggttcaggc ccagggagtg
     5161 cgcaacggcg atctgctctt tgccgcggcc gatcaggctc ttggcgagac gaatggtaac
     5221 tttctccatt gtagtaccct ccttagccca agatctcggc aacggtcttg ccgcgcttag
     5281 cagcgacgct ctcggcgtcg gtcaggccgc acaggcctgc gaaggtagca gcagtgacgt
     5341 tgatggggtt gttggaacgc aggctcttgg cgcggatgtc cttgatgccg gcgcactcga
     5401 gcacggcacg gacaggaccg ccggccagaa cgccggtacc gggggcagcg ggacgcatca
     5461 ggacgcggcc tgcgccgaac tcgccgacga tctcgtgagg aatggtggtg cccttcaggc
     5521 agaccttgcg catgttcttc ttggcagcgc cctcaccctt gcggatagcc tcgggaactt
     5581 cgccggactt gccgacaccg tagccgatgt tgcccttgcc gtcgcccacg acgatcagag
     5641 cagcgaactt cataacgcgg ccgcccttga cagtcttgga aacgcggttg atggagacga
     5701 ccttagtgat catgccgtca tcttcttgtc ttctctgcat ggccattgta tcttctccct
     5761 ccttacagct tcagaccggc ctcgcgggca ccctcagcga gagcctgcac acggccgtgg
     5821 taaacgaagc cgccacgatc gtagacgacc tcagtaatgc ccttctccag gcacttcttg
     5881 gcgatggcct cgccaacctt cttagcagcg tcaatattgc cgccgtaagc attgaaatct
     5941 ttgtccatgc tggacgcgct ggccagggtc acgccctgct catcatcgat gacctgagcg
     6001 tagatgtgct tggcagagcg gaaaacatcc agacggggac gagcagcagt gccgctgatc
     6061 ttgccgcgga cacgacggtg gcggcgcaga cgagcaacgt tcttatcagc cttgttgacc
     6121 ataatgtttc tcctctccta ttatttaccc ttgccggctt tgccttcctt acggatgaca
     6181 tactcgccaa catagcggat acccttgcct ttgtacggct cgggcgggcg gacaccgcgg
     6241 acctcggcag cgaactggcc gacttcctgc ttgtcgatac cggtgatgct gaagtgcagc
     6301 gcatctttca gctcaatctt gatggtatcg gtgtcctcga actcaacggg atgagagaag
     6361 cccagggtca g
//
LOCUS       DBMDSQ010000090         6363 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_3112_length_6363_cov_1.901789, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000090 DBMDSQ010000000
VERSION     DBMDSQ010000090.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 6363)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6363)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6363
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3112_length_6363_cov_1.901789"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..988)
                     /locus_tag="ACZPAX_04680"
     CDS             complement(<1..988)
                     /locus_tag="ACZPAX_04680"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HIR1113226.1"
                     /translation="MSLQSFGFFGFLLVVAAVYLHLPQRWQSAFLLAASWVFYALAMP
                     SMLGVTIAIAVFTYLCGRGMAARSSAHKTAFLRGGVIGLLAILAFFKYNGAFASEGGW
                     QAVAMPLGISFYSFAAIAYLIDTARGDCEVETNFIDCALFLNFFATVTQGPICRAGAL
                     LPQFKQEHRFDTDRTVRALRLMALGLFKLVAVSDVLGLLVDEVFPNYRSYGGPMLVLA
                     AVFYTFQLYFNFSGYSEVARAVGLLLGLELPENFKTPFFATNFSGFWSRWHISFSSWL
                     QDYLFMPLAWADMSGVTGGRLTRLPAEVCVFTVFFVSGFFSIIGFYAEIHING"
     gene            1122..2441
                     /locus_tag="ACZPAX_04685"
     CDS             1122..2441
                     /locus_tag="ACZPAX_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007048231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HIR1113227.1"
                     /translation="MSYIRSAISKFLKTQPPARIIALGFALLILCGAALLMLPFAVHP
                     GLHIAPLDALFTATSAVCVTGLVVVDTADTFSMFGQVVIAVLIQIGGLGVASIGMGLA
                     LVTGRRINLKGRSLVREALNVESLDGMVRLVRAVLLMTLICEGAGAVLGFPAFAGNHM
                     PLQAVWLSIFHSIASFNNAGFDALGGGTNLIPYRGDVLLNIVTDALVIVGGIGFMVIL
                     DVGRCRGNFRKMTFHTKVVLTTTAVLLFGGAALLQITDHLDFMTAFFQSMTARTAGFS
                     TVDLSGLSSAGLLVIMVLMFVGASPGSTGGGIKTTTFFVLMQQVRSIFSKRRLGAFHR
                     RLPDGSLAKAATITLMGLIVVSCGTFALCILEPETDFLRLLFEQVSAYSTAGLSTGIT
                     AGLRAGSKAVLIFTMFIGRVGAVTLLSLWVERPEPSAHYTEETITIG"
     gene            2563..3234
                     /locus_tag="ACZPAX_04690"
     CDS             2563..3234
                     /locus_tag="ACZPAX_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HIR1113228.1"
                     /translation="MFDKKKNSAESYGVIGLGRFGTALVKTLAAAGKEVIAVDKDEAK
                     VKAVRGYTDYAFVIDELDEVSLKETGMQNCGTVTICIGEQVDVSILTTMLVTKLGVPH
                     VISKAASEVHGEVLKRLGAEVVYPEADMAVRIGKRLISGNLLDYIALGDGVEVRRIAV
                     GGKMLGKSVRQLDVRKVYGINIIAVEHGNQTNVEFAADYTFKEGDSVAVIGKVGKIDK
                     FEKEI"
     gene            3384..4472
                     /locus_tag="ACZPAX_04695"
     CDS             3384..4472
                     /locus_tag="ACZPAX_04695"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005945787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HIR1113229.1"
                     /translation="MEHSNPILMGNSSPQKFITIGEVMLRLTPPNYEKIRMTSTFEAS
                     YGGSEANIALALANLGVDSTFFSVVPNNSLGKSAIRWLRCNDVHCTPMILSTKEETPS
                     HRLGTYYLETGYGIRPSKVIYDRMHSALTEYDLTKVDLDELFDGFDWLHLSGITPALN
                     KNCADFILRCLRTAKEKGLTVSFDGNFRSTLWSWEDARDYCTQCLPYVDVLFGIEPYH
                     LWKDEEDHAKGDWKDGVPLQPSYEQQDEIFQHFIDRYPNLKCIARHVRYVHSGSENSL
                     KAFMWYEGHTFESKLYTFNILDRVGGGDAFASGLIYAMMHDYKPMDMVNFAVASSAIK
                     HTIHGDANITDDVESIRNLMNMNYDIKR"
     gene            complement(4709..5491)
                     /locus_tag="ACZPAX_04700"
     CDS             complement(4709..5491)
                     /locus_tag="ACZPAX_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015565683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HIR1113230.1"
                     /translation="MLTSAFIGILIPFAGTTIGSACVFFMKKSLHPGVQRALTGFAAG
                     VMVAASIWSLLSPAMEQSAALGRLAFLPAVIGFWLGVGFLLLLDVLVPHLHQGADTPE
                     GPKSGFSRTTMLVLAVTLHNIPEGMAVGVVYAGYLTGSAQITAMGALALSLGIAIQNF
                     PEGAIISMPLKAEGMSKGKAFLMGTLSGAVEPLGALFTILAAGLVVPALPYLLSFAAG
                     AMLYVVIEELIPEMSQGQHSNVGTICFALGFTLMMALDVALG"
     gene            5750..>6363
                     /locus_tag="ACZPAX_04705"
     CDS             5750..>6363
                     /locus_tag="ACZPAX_04705"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015538191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HIR1113231.1"
                     /translation="MGLGKKTIDDENYAGKRVLVRCDFNVPMKDGVITNDNRINAALP
                     TIKKLINDGAKVILCSHLGKPKNGPEAKFSLAPVAVRLSEKLGQPVVFADDDTVVGEN
                     AKAAVATMGNGDVVLLQNTRFRKEETKNEPAFSEELASLADAYVDDAFGSCHRAHCST
                     AGVTDYVKDTAVGYLMEKEIKYLGNAVNDPERPFTAILGGAKVA"
BASE COUNT         1426 a         1898 c         1826 g         1213 t
ORIGIN      
        1 gaccattgat gtgaatttcg gcatagaaac caataatgga gaagaaaccc gacacaaaaa
       61 acaccgtaaa cacgcacacc tcggcgggca ggcgggtcag cctgcccccg gtcacgccgg
      121 acatatccgc ccacgccagc ggcatgaaca gataatcctg cagccaagac gaaaagctaa
      181 tgtgccaccg gctccagaac ccggaaaaat tcgtggcaaa aaacggcgtc ttgaagtttt
      241 caggcagctc caggcccaaa agcagaccca cggcccgcgc gacctcggag tagccggaga
      301 agttgaaata cagctggaac gtgtaaaaca cggcggccag caccagcatc ggcccgccgt
      361 agctgcggta attcgggaac acctcgtcca ccagcagccc cagcacgtcg ctgacagcca
      421 ccagcttaaa caggcccagc gccatgagcc gcagcgcgcg cacagtgcgg tcggtgtcaa
      481 agcggtgctc ctgcttaaac tgcggcagca gcgccccggc gcggcagata ggcccctgag
      541 tgacggtggc aaaaaagttc aaaaacagcg cacaatcgat gaaatttgtc tcgacctcgc
      601 agtcgccccg cgcagtgtcg atcaggtagg caatggccgc aaagctgtaa aagctgatgc
      661 ccagcggcat ggccacggcc tgccagccgc cctcgctggc gaacgcgccg ttatatttga
      721 aaaaggccag aatcgccagc aacccgataa cgcccccgcg caggaacgca gtcttgtggg
      781 cgctgctgcg ggccgccatg ccgcgcccgc acaggtaggt gaacaccgca atcgcgatcg
      841 tcacgcccag catcgacggc atggccagcg cgtagaaaac ccagctggcc gccagcaaaa
      901 acgcgctctg ccagcgctgc ggcaggtgca ggtacaccgc ggccacgacc agcagaaaac
      961 cgaaaaatcc aaaactttgc aggctcatgg ccacacctcc cctttataat cagtaataac
     1021 ttattataca tgatttgccg tgggtacgca agtgggttgt tgtggtgcag cgtgatttgt
     1081 gctatactat cggtaaagct aaatttttgc cggaggccgc tatgtcctac atccgcagcg
     1141 caatctcaaa atttttaaag acccagcccc cggcgcgtat catcgcgctg ggctttgcac
     1201 tgctgatttt gtgcggtgcg gcgctgctga tgctgccctt tgcggtacac ccggggctgc
     1261 acattgcccc gctggacgcc ctgttcacgg ctaccagcgc agtttgcgtc acgggacttg
     1321 tggtggtgga taccgccgac accttcagta tgttcggcca ggtcgtcatt gcggtgctca
     1381 ttcaaatcgg cggcttgggt gtcgcatcca tcggcatggg gctggcactg gtgaccgggc
     1441 ggcgcatcaa cttaaagggc cgctccctcg tgcgggaggc actgaacgtg gaaagtctgg
     1501 acggcatggt ccgccttgtc cgcgcggtgc tgctcatgac cctgatttgc gaaggcgcgg
     1561 gcgcggtgct aggtttcccg gcgttcgccg gcaaccatat gccgctgcag gcggtgtggc
     1621 tgagtatttt ccactcaatc gcttccttta acaatgcggg ttttgacgcg ctgggcggtg
     1681 gcacaaacct gataccctac cgcggcgatg tgctgctgaa tatcgtcacc gacgcgcttg
     1741 tcatcgtggg cggcatcggc ttcatggtca ttctggatgt gggacgctgc cggggcaatt
     1801 ttcgcaaaat gaccttccac accaaggtcg tgctgacgac gacggcagtg ttgctgttcg
     1861 gcggcgcggc gctgctgcaa atcaccgacc atcttgactt tatgacggcg tttttccaaa
     1921 gcatgacggc ccgcacggcg ggtttctcca ctgtggatct cagcggcttg agcagtgcgg
     1981 gcctgttggt cattatggtg ttgatgttcg tgggtgcgtc gccagggtcc acaggcggcg
     2041 gcatcaagac gaccaccttt tttgtgctga tgcagcaggt gcggtcgatc ttctccaagc
     2101 gccggctggg cgcattccac cgccgcctgc cggacggctc actggcaaag gctgccacca
     2161 tcaccttgat gggcctgatc gttgtgagct gcggtacctt cgcgctctgt attttggagc
     2221 cggagacgga tttcctgcgc ctgctgtttg agcaggtgtc ggcgtacagc acagcggggc
     2281 tttccaccgg catcacggcg gggctgcggg ccggcagcaa agcagtgctg atttttacca
     2341 tgttcatcgg ccgcgtgggt gcggtgacgc tgctctccct ctgggttgag cgccccgaac
     2401 ccagcgccca ttacaccgag gaaaccatca cgattgggta aacggcgcgg cgaggtgagg
     2461 gcaccccgcc ctacaaatac ggaaaaccgt gtgccgtagg gaggcatgcc cccatgccgc
     2521 cgtggacgct tgccgcagca cattcagaaa ggtattgccg ctatgtttga caaaaagaaa
     2581 aattctgccg aaagttacgg cgtcatcggc ctgggtcggt ttggtacggc gctggtcaaa
     2641 acgctagctg ccgcgggcaa agaggtcatc gccgtggaca aggacgaagc caaggtcaag
     2701 gccgtgcgcg gctacaccga ttacgccttt gtcatcgatg agctggacga agtatcactg
     2761 aaagaaaccg ggatgcagaa ctgcggcacc gtcacgatct gcattggtga gcaggtggat
     2821 gtcagtattc tgactacgat gcttgtcacg aagctgggcg tgcctcacgt tatttccaag
     2881 gccgccagcg aggtccacgg cgaagtgctc aagcgcctgg gggccgaagt cgtctacccc
     2941 gaggctgaca tggccgtgcg catcggcaag cggctgatct cgggtaatct gctggattac
     3001 attgccctgg gcgacggcgt cgaagtgcgg cgcattgccg tgggcggcaa gatgctgggc
     3061 aaaagcgtcc gccagctgga tgtgcgcaaa gtctacggca tcaatatcat cgccgtggag
     3121 cacggcaacc agaccaatgt ggaatttgcc gcggactata cctttaagga gggggacagt
     3181 gtggcggtaa tcggcaaggt cgggaagatc gataagtttg agaaggaaat ataaagaagg
     3241 cccccccttg acaaggaagg ttttgttccc ctcttgttaa aaaactaacc gcacaaattt
     3301 tgcgcatttt accttgcaat tcggcctgta atgcggtaca cttaaactaa caaaatatgg
     3361 caaaaacagg aggacttccc cccatggaac acagcaatcc gatcctgatg ggcaacagca
     3421 gcccgcagaa gtttatcact atcggcgagg tgatgctgcg cctgaccccg ccgaactatg
     3481 aaaagatccg catgacttcc actttcgagg ccagttacgg cggcagcgag gcaaacattg
     3541 cgctggctct ggcgaacctc ggcgtggatt ccaccttctt cagcgttgtg cccaacaact
     3601 ccctgggcaa aagcgccatc cgctggctcc gctgcaatga cgtccactgc acgccgatga
     3661 tcctctccac caaggaagaa acccccagcc accgcctggg cacctattac cttgagaccg
     3721 gctacggcat ccgccccagc aaggtcatct atgaccgtat gcacagtgcc ctgaccgagt
     3781 acgacctgac caaggtcgat ttggatgagt tgtttgacgg cttcgactgg ctgcacctgt
     3841 ccggcatcac cccggcgctg aacaaaaact gcgccgattt tatcctgcgc tgcctgcgca
     3901 ccgccaagga gaagggcctg actgtcagct ttgacggcaa cttccgttcc acgctgtggt
     3961 cctgggagga tgcccgcgac tactgcaccc agtgcctgcc ctacgttgat gtactgttcg
     4021 gcatcgagcc gtaccacctg tggaaggacg aagaggatca tgctaagggt gactggaagg
     4081 acggcgtccc tctgcagccc agctatgagc agcaggacga aattttccag catttcatcg
     4141 accgttaccc caacctcaag tgcattgccc gccatgtgcg ctatgtccac tccggcagtg
     4201 agaacagcct gaaagccttt atgtggtacg agggtcacac gttcgagtcc aagctctata
     4261 cctttaatat tctggatcgt gtcggcggcg gcgatgcctt tgccagcggc ctgatctacg
     4321 ccatgatgca cgactataag ccgatggata tggtcaactt cgcggtcgcg tccagcgcca
     4381 tcaagcacac catccacggc gatgccaaca ttaccgacga cgtggagagc atccgcaatc
     4441 tgatgaatat gaactacgat atcaagcggt aatacgtaaa ataaagcgcc gtcccgggtg
     4501 agggacggcg tttttatttg ggaaggtgtc cgctgcccgc ccggcacgta ggtgtaaatc
     4561 agcagcagcc cgaacagcag cggcaccgcc acgatctgct cgatgctcgc aatgcgctgt
     4621 aactggtgga cgacgggccg cacatatact gcccctacac ctctgcctgg cggtttttac
     4681 ctattatact gctgccgcca aacgcaagtc accccagcgc cacatccagc gccatcatca
     4741 gggtaaagcc cagcgcaaag cagatcgtgc ccacgttgga gtgttgaccc tgggacattt
     4801 cggggatcag ctcctcgata acaacataca gcatcgcgcc cgcggcaaag ctcaaaagat
     4861 acggcagcgc gggcacgacc agccccgccg ccaagatcgt gaacagtgcg cccagcggtt
     4921 ccaccgcgcc ggacagtgta cccatcagga acgccttgcc tttgctcatg ccctcggctt
     4981 tcaaaggcat tgaaatgatc gcaccttcgg ggaaattctg gatggcaatg cccagggaca
     5041 gcgccagtgc gcccatggcg gtgatctgcg cgctgccggt taagtatccc gcgtacacaa
     5101 cgccgacggc catgccctcc gggatattgt gcagcgtgac ggccagcacc aacatcgtcg
     5161 tgcgggaaaa gccacttttg gggccttcgg gcgtgtccgc gccctggtgc aggtggggga
     5221 cgagcacatc cagcagaagc aggaacccga cgccgagcca gaacccgata acggcgggca
     5281 ggaacgccag ccgccccagc gcggcggact gctccatggc gggggagagc aggctccaga
     5341 tggatgccgc caccatgacc ccggcggcaa acccggtcag cgcccgctgc acgccgggat
     5401 gcagggactt tttcataaag aacacgcagg cagacccgat agtcgtgccc gcaaagggga
     5461 tgaggatccc aatgaatgcg cttgttagca tacactaacc ctcctgtata aatcatagta
     5521 aaatagtatg ctttcgtaag ggattatagc acaaaggggg aggggcgtca aggcacaggg
     5581 ggaaagcccc tcccgacaca aaaattttac acccccttca cacaaagcag ttaaaaatat
     5641 atcaaaaaca cttgcgcaaa tcgaactttg tgcgtataat aatactaaac cccttggggt
     5701 gcggtgcgcc ccgccaaaaa tgtgaaacat accgagagga gcttttacta tgggtttggg
     5761 taaaaagacc attgacgatg agaactacgc cggtaagcgc gtactggttc gctgcgactt
     5821 caatgtcccg atgaaggacg gcgttatcac caatgataac cgcatcaacg ccgcactgcc
     5881 gaccatcaag aaattgatca acgacggcgc taaggtcatc ctgtgcagcc atctgggcaa
     5941 gccgaagaac ggccccgagg ccaagttcag cctggccccc gttgctgtcc gcctgagcga
     6001 gaagctgggc cagccggtcg tctttgcgga cgatgacact gtcgtcggcg agaatgccaa
     6061 ggctgctgtt gccaccatgg gcaacggcga cgtcgttctg ctgcagaaca cccgcttccg
     6121 caaggaagag accaagaatg agcccgcttt cagcgaagag ctggccagcc tggccgacgc
     6181 ttatgtcgat gacgccttcg gctcctgcca ccgtgcccac tgctccaccg ccggtgtgac
     6241 cgactatgtc aaggacaccg ctgtcggcta cctgatggag aaggagatca agtacctcgg
     6301 caacgccgtg aacgatcccg agcgtccctt caccgccatt ctgggcggcg ctaaggtcgc
     6361 tga
//
LOCUS       DBMDSQ010000091         6325 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_3129_length_6325_cov_1.799486, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000091 DBMDSQ010000000
VERSION     DBMDSQ010000091.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 6325)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6325)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6325
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3129_length_6325_cov_1.799486"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(44..2068)
                     /locus_tag="ACZPAX_04710"
     CDS             complement(44..2068)
                     /locus_tag="ACZPAX_04710"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HIR1113232.1"
                     /translation="MKTITKAPILFRGGDYNPDQWLDRPDILEKDVELMKKAGMNTAT
                     LGVFAWAKYEPQEGEYDFAWLREMMDRLYAAGIYTELATPCGAKPNWMAKKYPEILRM
                     QANGVTDHQGMRHNACPSSLIYREKVHNIIEKLVEAVGDHPGLILWHISNELGGDCYC
                     PRCQARFRDWLRDKYKTIDALNHAWWTGFWSHHYNDFDEIEPPFENGEQSLLGLKLDW
                     RRFTTWNMTDYVHSETELLHKLTPNVPITTNLMEYFPGLDYHYLQKELDFVCWDSYPH
                     WGRPDRSITTTFAMTAFDHALIRGCKRDVPFFTMESTPSLVNWHEYNKLKRPGVNRLQ
                     GLQTVACGGDGVQYFQWRKGRGGTEQWHGAVVDHDGRDDTRVFKDVTETNAALNALTP
                     VIGSLPRAEAALIFDWDSRWALEDAWGMQIQQKNLRETVCALHEQLGRCGVDADVIGV
                     EESLDRYKVVVLPMLYMVKPGFGEKIRQFVANGGTVIGTYLLGYVNDSTLAWLGGFPG
                     DGLREVFGVTATELDTLYPGERNTVVFPDGSKAAIQDYCELLETADNTDVLATYGEDF
                     YKGYAVATHHAFGKGHAYYVAARMDADGNAKVFRAAMAQAGCTMQTLPEGVEYHCRED
                     ERTVYHFYLNTTETDKTVAVPTGADLLTSKTVSGEVTLGRYGACAVEVVK"
     gene            complement(2195..3094)
                     /locus_tag="ACZPAX_04715"
     CDS             complement(2195..3094)
                     /locus_tag="ACZPAX_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007048066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HIR1113233.1"
                     /translation="MSTTTINDAAVQKQLKQGRVKKTIFSIIRHLILALLAFIWLVPI
                     AWLLLTSFSTDKGINFTRFVPEGFTLWNYISIMTAPDSVAQFPRWFMNTLVVACFNCV
                     ISTCFVLMVAYAISCCRFKGRRLLQNLSVTVNLFPGVLAMIAVYFVLKYMNLTNSYAG
                     LIMVYAGSSGLGYLICKGFFDTVPVSLREAAKLDGASEARIFFQIVIPMSRPILVYTI
                     INSFLIPWTDFVMAKMILNSGISTDWTVAIGLYNMLQKALINSYFSRFCAGGVLVAIP
                     ISILFVIMQKFYVEGITSGAAKG"
     gene            complement(3091..4527)
                     /locus_tag="ACZPAX_04720"
     CDS             complement(3091..4527)
                     /locus_tag="ACZPAX_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007048067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR1113234.1"
                     /translation="MAKTATRAMPGEKNFFQTFGEAFVKGDIFTKVSLLVWGLGYIGH
                     GQLMKALLVTLVEGLGLYFLAFYGAPNLAKFGTLGTVKMEMVFDVTTMKNVVNNYDNS
                     FEILLMSMISFVVIAALIAAAMMVVTSNYQLQKIRAAGKKTNNFLQDINVYLNEKFYV
                     TLLTLPVLGVVVFTIVPLFILIAVAFTNYDQQHMPPNALFTWVGLTNFFTLFGGGGMT
                     STFGYSFAKVLTWTLVWAFFATFTTFFGGILLAMFINDQKTKCPKFWRTLFMVTIAVP
                     QFVTLLLVRNFFSNNGIFNTMMANAGVTDFLHNIGLLDKGLSYIPFLTQPGWAMFTII
                     MINIWIGVPYQMLISTGVLMNIPADMIEAARIDGANPVQMFFRIKLPYLLSVQGPSLV
                     TDFVRNVNNFNVIYLLTQDVFVTNNQQLAQSNAKEIDLLVTWLFRLTQEYYNYKMAAV
                     LGIMVFIVCAVFTVVCFHFINKKEATFS"
     gene            complement(4666..5988)
                     /locus_tag="ACZPAX_04725"
     CDS             complement(4666..5988)
                     /locus_tag="ACZPAX_04725"
                     /inference="COORDINATES: protein motif:HMM:NF024808.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HIR1113235.1"
                     /translation="MKKQISLALAGVMALSLAACGGSASTATSTSEAASTAESTEASS
                     AASTEAAAAGDVLDQAAAAAFAQDVTLTMWGAEEDQDLLREISDKFIEKYGNYGGKIT
                     INLGAQSEKEAKDTVLTDPTAAADVFAFADDQLNELVQAGALQEVQLNADDIKSRNTP
                     ASVDAATVDGKLYAYPLTADNGYFMFYDKSFFTEDDVKSLDTMMDKAAAAGKKVSMDV
                     ANGWYLYSFYAGAGLNLALADDGVNTVCNWNEAPGADVTQAVIDICKNPGFIALKDEE
                     FTGKLKDGTLVAGVNGTWRANDAAEAWGDNYAACKLPTYTLNGEQVQMASFSGYKLIG
                     VNPHSANVGAAMLLADFVTNEENEELRFKERAQGPSNTNALAAASSPALTAVVDQSEY
                     ANLQRVGGNFWASAETLGSICVNGNPDGKDTQTLVDDAVAGITAPVTQ"
BASE COUNT         1355 a         1716 c         1887 g         1367 t
ORIGIN      
        1 cctcgctcgc cgtatcggca cttagaatta tgcggtattg cctttacttt acaacttcca
       61 ccgcgcaggc accatagcgg cccagggtta cttccccgct gacggttttg ctggtcagca
      121 ggtcggcccc ggtgggcaca gccacggtct tgtccgtttc ggtcgtgttc aggtagaagt
      181 gatacaccgt gcgctcgtcc tcgcggcagt ggtactcaac gccctcgggc agggtctgca
      241 tcgtgcagcc tgcctgcgcc atggcagcgc ggaacacctt cgcgttgccg tcggcatcca
      301 tgcgggccgc aacgtagtag gcgtggccct tgccgaaggc gtggtgggtc gccacggcgt
      361 aacccttgta gaagtcctcg ccgtaggttg ccagcacatc ggtgttgtcc gccgtttcca
      421 gcagctcgca atagtcctgg atggcggcct tgctgccgtc cgggaagacg acggtgttgc
      481 gctcgccggg gtagagggtg tccagctcgg tggcggtcac gccgaacacc tcccgcaggc
      541 catcgccggg gaagccgccc agccaggcca gcgtagaatc attgacatag cccaacaggt
      601 aggtgccgat aaccgtgccg ccgtttgcca caaactgacg aatcttctca ccgaagccgg
      661 gcttgaccat gtagagcatc ggcagcacga cgactttgta gcggtcgaga gattcctcga
      721 cgccgatgac atcggcatcg acgccgcagc ggcccagctg ttcatgcagg gcgcagacgg
      781 tctcgcgcag gttcttctgc tggatctgca taccccaggc atcttccagc gcccagcggc
      841 tgtcccagtc aaaaatcagc gcggcctcgg cgcggggcag gctgccgata accggcgtca
      901 gcgcgttcag cgcggcgttc gtctcggtca catccttgaa cacccgggta tcgtcgcggc
      961 catcgtggtc cactaccgcg ccgtgccact gctcggtgcc gccgcggccc ttgcgccact
     1021 ggaagtactg tacgccgtcg ccgccgcagg cgaccgtctg caagccctgc agtcggttga
     1081 cgccggggcg cttgagcttg ttatactcgt gccagttgac gaggctgggc gtgctctcca
     1141 tcgtgaagaa aggtacgtcc cgcttgcagc cgcgaatcag cgcgtggtca aacgcggtca
     1201 tggcaaaggt cgtcgtgatg ctgcggtcgg ggcggcccca gtgggggtag ctgtcccagc
     1261 agacgaaatc cagttctttc tgcaaatagt ggtagtccaa cccggggaaa tactccatca
     1321 ggttggtggt gatgggcacg ttcggggtca gcttgtgcag cagctcggtc tcgctgtgga
     1381 catagtcggt catgttccag gtggtgaagc gccgccaatc cagtttcagg cccagcaggc
     1441 tctgttcgcc gttctcaaac ggcggctcga tctcgtcaaa atcgttgtag tggtggctcc
     1501 agaagccggt ccaccaggcg tggttcagcg cgtcgatggt cttgtacttg tcgcgcagcc
     1561 aatcgcggaa gcgggcctgg cagcgggggc agtagcagtc gccgcccagc tcattggaga
     1621 tgtgccacag aatcagcccg gggtggtcac cgacggcctc gacgagcttt tcaatgatgt
     1681 tgtggacctt ctcgcggtag atgaggctgc tggggcaggc attgtggcgc ataccctggt
     1741 ggtcggtcac gccgttggcc tgcatgcgca ggatctcggg atattttttc gccatccagt
     1801 tgggcttagc gccgcagggg gtggcaagct cggtgtagat gcccgccgcg tacaggcggt
     1861 ccatcatctc gcgcagccag gcaaagtcgt actcaccctc ctgcggctcg tacttggccc
     1921 aggcgaacac gccgagggtg gcggtgttca tgccggcctt tttcatcagt tcgacgtcct
     1981 tttccaggat gtcgggccgg tcaagccact ggtcggggtt atagtcccca ccgcggaaca
     2041 ggatgggggc tttggtgatc gttttcatgg tgaacctctt tactttaaaa taaggtgcct
     2101 tgtagtggcc gggcatatcc ggccactgcg ttaaatctgc ccttcaccca caaaagcgct
     2161 gccccttgcg aggcagcgtt gttgtgataa actatcagcc ctttgccgcg ccggaggtga
     2221 taccctcgac gtagaatttc tgcatgatga caaagaggat ggaaatgggg atggcgacca
     2281 gcacaccgcc cgcgcagaaa cgggagaagt agctgttgat cagagccttt tgcagcatat
     2341 tgtacaggcc gatggcaacc gtccagtccg tgctgatacc ggagttcagg atcatctttg
     2401 ccataacaaa gtcggtccag ggtatcagga aggagttgat aatggtgtac accaaaatcg
     2461 ggcgggacat cgggataacg atctggaaga agatgcgcgc ttccgaagcg ccgtccagct
     2521 tggcggcctc acgcagggaa accggaaccg tgtcgaaaaa gcccttgcag atcaggtagc
     2581 ccaaaccgga ggaaccggca tagaccatga tcagtcccgc gtaggaattc gtcaggttca
     2641 tgtacttcaa gacgaagtaa actgcgatca tggccagaac gcccgggaac aggttgaccg
     2701 tgacggacag gttttgcagc agcctgcggc ccttgaagcg gcagcagctt atggcgtaag
     2761 ccaccatcag cacaaagcag gtggagatca cgcagttaaa gcaggccacg accagggtgt
     2821 tcatgaacca gcgcgggaac tgtgcaacag agtccggcgc tgtcataatg ctgatgtagt
     2881 tccacagggt aaagccttcc ggcacaaagc gggtaaagtt gatgccctta tcggtggaga
     2941 acgaggtcag cagcagccaa gcaatgggca ccagccagat aaaggccagc agcgccagga
     3001 taagatggcg gatgatggag aaaattgttt tcttgacgcg gccctgtttg agttgcttct
     3061 gcacggcggc atcgttgatg gtagtggtac tcaagagaac gtcgcctcct tcttattgat
     3121 aaagtggaag cacacaacgg tgaacactgc gcagacgata aacaccatga tgcccagcac
     3181 agcggccatc ttgtagttgt aatactcctg cgtcaagcgg aacagccagg taaccagcag
     3241 gtcaatttcc ttggcgttgg actgggccag ctgctggttg ttcgtgacaa acacgtcctg
     3301 ggtcagcagg taaataacgt tgaagttgtt gacgttcctg acaaagtccg tgacgagcga
     3361 cggtccctgc acgctgagca ggtagggcag cttgatgcgg aagaacatct gaaccgggtt
     3421 cgcaccgtcg atgcgggcgg cctcgatcat gtcggcggga atgttcatta acacacccgt
     3481 agaaatcagc atctggtacg gtacgccgat ccaaatgttg atcatgatga tggtgaacat
     3541 ggcccagccc ggctgggtca ggaacggaat gtaactaagg cccttgtcca acaggccgat
     3601 gttatgcagg aaatccgtaa ctccggcgtt ggccatcatt gtgttgaaga tgccgttgtt
     3661 ggagaagaag ttgcgtacca gaagcagcgt aacgaactga ggcacggcga tggtcaccat
     3721 gaacagggta cgccagaact tcgggcattt ggtcttttgg tcattgatga acatggccag
     3781 caggataccg ccgaagaagg tggtaaaggt ggcaaagaac gcccagacca gcgtccaggt
     3841 caacaccttg gcgaaggagt agccgaaggt cgaggtcata ccgccgccgc caaacagcgt
     3901 gaagaagttg gtcaggccca cccaggtgaa cagggcgttc gggggcatat gctgctggtc
     3961 atagttcgtg aacgcgaccg caatcaagat gaacagcggc acgatggtaa ataccacaac
     4021 gcccagcaca ggcagggtca gcagcgtgac atagaacttt tcattcaggt agacgttgat
     4081 gtcctgtaag aagttgttcg tctttttgcc cgctgcgcgg attttttgca gctggtagtt
     4141 ggaggtgacc accatcatgg cggccgcgat cagcgcggca atgaccacga aggaaatcat
     4201 gctcatcagc aggatctcga aggagttatc gtaattgttc acgacgtttt tcatcgtcgt
     4261 aacgtcgaac accatctcca ttttgacagt gcccagcgtg ccgaatttag caaggttcgg
     4321 cgcaccgtaa aatgccagga agtaaagtcc cagaccctcc acgagggtga caaggagcgc
     4381 tttcatcagc tggccgtgac cgatatagcc aaggccccat accaggagcg aaactttggt
     4441 aaagatatcg ccttttacaa aggcttcccc aaaagtctgg aagaagttct tctcgcccgg
     4501 catggctcgc gttgcagttt tcgccacagc gaagtcccct ttcgttcaca cgatcttcat
     4561 aaatcatatg gataaaacag cccgcttgca tcggtcgttt tctaccacaa gcgggctgtc
     4621 ttatcagatc atttctttat taattattat agataggaac agagattact gggtaacagg
     4681 agcggtgata ccggcgacag cgtcatcgac caaagtctgg gtgtccttgc catcggggtt
     4741 gccgttgacg cagatggagc cgagggtctc agcggaagcc cagaagttgc cgccaacgcg
     4801 ctgcaggtta gcgtactcgg actggtcaac aacagcggtc agagccggag aagaagcggc
     4861 tgccagagcg ttggtgttgg aggggccctg tgcgcgctcc ttgaagcgca gttcctcgtt
     4921 ctcttcgttg gtaacgaagt cggccagcag catagcagca ccgacgttag cgctgtgggg
     4981 gttgacaccg atcagtttgt agccggagaa ggaggccatc tggacctgct caccgttcag
     5041 ggtgtaggta ggcagcttgc aggcggcgta gttgtcgccc caagcttcag cagcgtcgtt
     5101 tgcacgccag gtgccgttga cgccagcaac caaagtgccg tccttcagct taccggtgaa
     5161 ctcctcgtcc ttcagggcga tgaaaccggg gtttttgcag atatcgatga cagcctgagt
     5221 gacatcagca ccgggagcct cgttccagtt gcagacggtg ttcacgccgt catcagccag
     5281 agccagattc aagccggcgc cagcatagaa gctgtacagg taccagccgt tagcaacgtc
     5341 catagaaacc ttcttgcctg cggcggctgc cttgtccatc atcgtgtcaa ggctcttgac
     5401 atcgtcctcg gtgaagaagc tcttgtcgta gaacatgaag tagccgttgt cagcggtcag
     5461 ggggtaagca tagagcttgc catcgaccgt ggcagcatcg acagacgcgg gagtgttgcg
     5521 gctcttgata tcgtccgcat tcagctgcac ttcctgcagg gcgcctgcct gaaccagctc
     5581 gttcagctgg tcatcagcaa aagcaaaaac atcagcagcg gcggtggggt cggtcaggac
     5641 ggtatccttg gcctcctttt cggactgagc gcccaggttg atggtgatct tgccgccgta
     5701 attgccgtac ttctcgatga acttgtcgct gatttcacgc agtagatcct gatcctcttc
     5761 agcgccccac atggtcaagg taacgtcctg agcgaaggca gcggcagcag cctgatcaag
     5821 gacatcgccg gcagcagcgg cctcggtgga agctgcgctg gaagcctcgg tggactcagc
     5881 agtggaagct gcttcggaag tggaggtggc ggtgctggca gagccgccgc aagcggcaag
     5941 ggacaaagcc attacaccgg ccagagccag gctgatttgc tttttcataa tgtactctcc
     6001 ttcttttaaa aatgttctct tgtgaacacc ttgagcggtg ctgcgcaact tgtttgcgca
     6061 ctcaccttac gttttttagg atacaggatt tttcgcttgg tgtcaatgta cagattatac
     6121 gtgtttcgta gtgcgttttt ggctgttttg ccgccggaaa cggcttattc gacgaaaaat
     6181 acgcgcaact tgcgcaactt ccgggttgca cacggttgtt tttggacaaa ttttccctgt
     6241 tttgtacagc aatctgacgt tgccgtaact attttgtaat ttgattcgat gatgtcataa
     6301 aataggaagt tgcgaagagc cggaa
//
LOCUS       DBMDSQ010000092         6302 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_3144_length_6302_cov_1.767296, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000092 DBMDSQ010000000
VERSION     DBMDSQ010000092.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 6302)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6302)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6302
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3144_length_6302_cov_1.767296"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(250..426)
                     /gene="rpsU"
                     /locus_tag="ACZPAX_04730"
     CDS             complement(250..426)
                     /gene="rpsU"
                     /locus_tag="ACZPAX_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005928271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HIR1113236.1"
                     /translation="MSEIRVKEGESLESALRRFKRSTARSGVLAEVRKREAYEKPSVK
                     RKKKSEAARKRKFK"
     gene            complement(665..1426)
                     /locus_tag="ACZPAX_04735"
     CDS             complement(665..1426)
                     /locus_tag="ACZPAX_04735"
                     /EC_number="2.3.1.78"
                     /inference="COORDINATES: protein motif:HMM:NF019403.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015019 - heparan-alpha-glucosaminide
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0007041 - lysosomal transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase"
                     /protein_id="HIR1113237.1"
                     /translation="MPVLRTKTNRMWLPDALRGLALLNMLVYHGMYDWVYVFGHASRW
                     YDIWAPGCHVWQQYICWSFIVLSGFSFPLARKPVKNGLLVAGCAAVLTAVTAVFMPSE
                     AIWFGVLHLNAAAVLLTCLVYPLLQRVPAGMGLATSAALFALTNQLPYGYLGFENWHL
                     CALPAGLYKANWFWLGLPDLTRFTSADYFPVIPWVFLFWCGVFLARLWHPSRGEAPAA
                     AAPLCVVGRNTLFVYMLHQPVIYGALWVWHRIRGM"
     gene            1538..2362
                     /locus_tag="ACZPAX_04740"
     CDS             1538..2362
                     /locus_tag="ACZPAX_04740"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HIR1113238.1"
                     /translation="MQDIRLVALDLDGTVFNDKKEITPRTLAAIRTACERGVAVLPAT
                     GRTASGVPEAFTAIPGVRYALTSNGASVVDLQTGEQLVNQPFKTEDALKIYDILEGGA
                     GMMSIFIGGKSYTTRENAEQHMDMVPENLRTYFRTTRLEVADMHKTLRDHAHEIEKYS
                     MIYATEAERDAAWHEVLAACPGMELTSSLPCNMEINAPGVTKGSGLMALARTLGLTRA
                     QVMAVGDSGNDCAMIEAAGLGVAMGNATDDIKKIADVTTDDNNHDGVAAAIENYVL"
     gene            2395..3615
                     /locus_tag="ACZPAX_04745"
     CDS             2395..3615
                     /locus_tag="ACZPAX_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HIR1113239.1"
                     /translation="MKNVLVGQSGGPTAVINSSLAGVYETAKACGAPHVYGMQYGIEG
                     VLEGKLVDLDTVLGDKMDIELLKRTPSSFLGSCRHKLPDPTVDEKPFLQLFELFKQYD
                     IGAVFYIGGNDSMDTISKLSAYGKTVNSEIRFIGVPKTIDNDLMLTDHTPGYGSAAKY
                     IATILKEVICDSSVYDLRSVTVAEIMGRHTGWLAAAASLAAGPDCNGPDLILLPERPF
                     DEEAFLARVAELEKERHNVIIAVSEGVKNKDGVFLCDLVSTAGQLDAFGHKAILSGTS
                     RYLADLIRVRLGCKTRAIEFSTLQRCASHLASRTDVTEAYQVGGAAVEAAVRGETAKM
                     AAIKRLSNQPYRVETEMVDVTKVANFEKTVPDEWITADGMHVNEKFERYARPLIQAEL
                     TPIYINGVPRHIHL"
     gene            complement(3842..4147)
                     /locus_tag="ACZPAX_04750"
     CDS             complement(3842..4147)
                     /locus_tag="ACZPAX_04750"
                     /inference="COORDINATES: protein motif:HMM:NF012442.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030527 - structural constituent of
                     chromatin [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HIR1113240.1"
                     /translation="MPIRIGVKNIMTRSQLITKVANDTNMTAAEVEKVMDGIFGTIGD
                     VLKSGDKVTIAGFGRFEMRERQTKNFTNPKTKVTSKLEPTAIPGFKASGRLKEKVSG"
     gene            complement(4490..4936)
                     /locus_tag="ACZPAX_04755"
     CDS             complement(4490..4936)
                     /locus_tag="ACZPAX_04755"
                     /inference="COORDINATES: protein motif:HMM:NF019849.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH32 C-terminal domain-containing protein"
                     /protein_id="HIR1113241.1"
                     /translation="MTISRVLTRDNAGHILQNPVSELAARRGAAVTATDGAEQAVDAC
                     FDLVAAPGGDFSLGFDGGLTLAYSDADRTCCLTFTDAAMSGGRTSRCVVLDAPCRRLR
                     VAGDASSLEIFLNDGAAVLSTRYYPAGGQVTLRPANFTAAVYPLNL"
     gene            complement(5181..>6302)
                     /locus_tag="ACZPAX_04760"
     CDS             complement(5181..>6302)
                     /locus_tag="ACZPAX_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR1113242.1"
                     /translation="RPSYVLGGQNMIVAYNSSNVIEYMGVITKHVDMSHPVLMDKYIL
                     GTECEVDAICDGTDYLVPGIMQQVERTGVHSGDSICVYPPTTFSQKVIDTLVDYTGRF
                     ARELKVVGLVNVQYAVQNDRVYVIEVNPRSSRTVPYISKVTGVPMVDLAVRCTLGEKL
                     KDMGYGTGLWRNGQSPYYAVKVPVYSFLKLHGVDTMLGPEMKSTGEVLGIAPTFHEAM
                     MKGLVAAGYQFKVPGPGSCVIISVKDSDKAEAAKLAWKLHDYGYKIYGTPGTAKYLNG
                     EMIPCSVVRQMSGARPNVLDLLESGLVDYIISTSPHGRDPHRDSVKFRRKATELSIPT
                     ITAMDTASVLVDALRGDHDISQMELVDIANLHREAANGK"
BASE COUNT         1335 a         1875 c         1873 g         1219 t
ORIGIN      
        1 tggacggatg gctccagcac ctggctgttg tcaccggtca gggtgttgtc tacgtccagc
       61 accagcgcct tgatgccatg tgccgcgaga aaatccgggt ggatgcactc gatggttttg
      121 aaaatatatt caggggttaa aatcatgggt gtgacctttt taaagatggc cgcatcgctg
      181 ccgccgataa aataaaagtg caggcccgcg cgtaacgcga acctgcacag tcgttcgttg
      241 taagatacct tacttaaact tgcgcttgcg ggcagcctcg gacttcttct tgcgcttcac
      301 ggaaggcttc tcataagcct cgcgcttgcg gacctcagct aacacgccgc tgcgagcagt
      361 gctgcgctta aaacggcgca gagcgctctc gagggactcg ccctctttga cacggatctc
      421 cgacatcttc ttccctccct tgacctgaga caggagtttg ccggtcaaat actattaacc
      481 agtaaacaag attatactat aaaagccccc ctgatgtcaa caacaatttt cgctaaaatg
      541 aaaattttta aaatgttcat aaatggtggg gtggtctgcc ctcaggccgc cgcactttgc
      601 ctttagaact actttagcct ttagttgtag ggggcggcgt cctcgtcgcc ccgtaccctc
      661 gccgtcacat ccccctgatc ctatgccaca cccacagcgc cccataaatc acgggctggt
      721 gcagcatata cacaaacagc gtgttccgtc caacaacaca gagcggcgct gccgcagcag
      781 gggcctctcc cctgctcggg tgccacagcc gcgccaaaaa cacgccgcac cagaacaaaa
      841 acacccacgg tatgaccggg aaataatccg ccgaggtgaa ccgcgtcaag tccggcaggc
      901 ccagccagaa ccagtttgcc ttgtacagcc ccgcaggcag ggcgcagagg tgccaattct
      961 caaagcctaa atacccgtag ggcagctgat tcgtcagcgc aaacagcgcc gcagacgtcg
     1021 ccagccccat cccggccggc acgcgctgca aaagcggata caccaggcag gtcagcagca
     1081 cggcggcggc gtttaggtgc agcacgccga accagatggc ttccgacggc atgaataccg
     1141 ccgtcacagc cgtgagcacc gccgcgcacc cggccaccag caggccgttt ttgaccggct
     1201 tgcgggccag cggaaagctg aaccccgaca gcacaataaa gctccaacag atatattgct
     1261 gccacacatg gcaccccggg gcccagatat cgtaccagcg gctggcgtgg ccgaacacat
     1321 atacccagtc gtacatgccg tggtagacca gcatattcag cagcgccagc ccgcgcaggg
     1381 cgtccggcag ccacatccgg ttggttttgg tgcgcaatac aggcatgtta ccgcctcttt
     1441 ctcaaatttt accgatactt tattgtagca cagttgcctg caccttgcaa tcggtgcggt
     1501 tttgcgatac aatattctgg ataaggagtg tgaaactatg caggacatcc gccttgtggc
     1561 gctggacctt gacggtactg ttttcaacga taaaaaggaa atcacccccc gcacgctggc
     1621 ggccatccgc acggcctgtg agcgtggcgt ggcggtgctg cccgctaccg gccgcacggc
     1681 aagcggtgtg ccggaggcgt tcaccgccat tcccggcgtg cggtacgcgc tgacctccaa
     1741 cggtgccagc gtcgtggacc tgcagaccgg tgagcagctg gtgaaccagc cgttcaagac
     1801 cgaggacgcg ctgaaaatct atgatatcct ggagggtggt gccggcatga tgagcatttt
     1861 catcggcggc aaaagctaca ccacccgcga aaacgccgag cagcacatgg acatggttcc
     1921 ggaaaatctg cgcacctact tccgcacgac ccgcctggaa gttgcggata tgcacaaaac
     1981 gctgcgcgac cacgcccacg agattgaaaa gtacagtatg atctacgcta ccgaagccga
     2041 gcgcgacgct gcctggcacg aggtgctggc cgcctgcccc ggcatggagc tgacgagcag
     2101 cctgccctgc aacatggaga tcaacgcccc cggtgtgaca aagggcagcg gcctgatggc
     2161 gctggcccgc acgctgggcc tgacccgggc gcaggtcatg gctgtgggtg actccggcaa
     2221 cgactgcgcc atgatcgagg ctgcgggctt gggtgtggcg atgggcaacg ccaccgacga
     2281 tatcaagaaa attgccgatg ttacgacgga tgataacaat catgacggtg tggcagctgc
     2341 gattgaaaac tatgttttat aatatatgaa ataaaatata cgagaggtaa acctatgaag
     2401 aacgttctgg tagggcagtc gggtggcccg actgctgtca tcaactccag cctggcgggt
     2461 gtctacgaga ccgcgaaggc ctgcggtgca ccccatgtct acggtatgca gtacggcatt
     2521 gagggcgtgc tggagggcaa gctcgttgac ttggacacgg tcctgggcga caagatggac
     2581 attgagctgc tcaagcgtac gccgtccagc ttcttgggaa gctgccgcca caagctgccc
     2641 gaccccacgg tggacgagaa gccgtttttg cagctgtttg agctgttcaa gcagtacgac
     2701 atcggcgcgg tgttctacat tggcggtaat gactcgatgg acacgatctc caaattgagt
     2761 gcctacggca agaccgtaaa cagcgagatc cgcttcattg gcgtacctaa gaccatcgac
     2821 aacgacctga tgctgaccga ccacaccccc ggctacggca gtgcagcgaa gtacattgcc
     2881 acgattttga aggaagtcat ctgcgattcc agcgtctacg acctgcgcag cgtgacggtg
     2941 gcggagatca tgggccgcca caccggctgg ctggccgctg ccgccagcct ggccgcaggc
     3001 ccggactgca acggccccga cctgattctg ctgcccgagc gcccctttga cgaggaagcg
     3061 ttcctggccc gcgtcgcaga gctggaaaag gagcgccaca atgtcattat cgcagtcagc
     3121 gagggcgtga agaataagga cggcgttttc ctgtgtgacc tggtctccac ggccgggcag
     3181 ctggacgcct tcggccacaa ggcaattttg agcggcacga gccgctacct ggcggacctg
     3241 atccgcgtgc gcctgggctg caagacccgc gccatcgaat tctcgacgtt gcagcgctgc
     3301 gccagccacc tggccagccg caccgacgtg accgaagcct accaggtggg cggcgcggcg
     3361 gtggaggccg ctgtgcgcgg cgaaaccgcc aagatggcag ccatcaagcg cctgagcaac
     3421 cagccctacc gcgtggagac cgagatggtc gatgtgacga aggtcgcaaa ctttgaaaaa
     3481 acggtccccg acgaatggat caccgcggac ggtatgcacg tcaacgagaa gtttgagcgc
     3541 tacgcccgcc cactgatcca ggccgaactg acgcccatct acatcaacgg cgtgccgcgg
     3601 catattcatc tgtgatagag caaaatgcct cccttgtcaa agggaggtgc cgagctatgc
     3661 gaggcgaagg gattcctgcc cggctgcggg cgtgagcgcg tgtaaggcaa cgtccgtcgg
     3721 aatccctccg gccgccttcg gtgtccacct ccctttaaca agggaggctt tttttgtttg
     3781 ttacaaacaa aaaaggaccc gcatccctgc ggatccttaa aagctatcaa tctgtacgag
     3841 atcagccgga gaccttctcc ttcaggcggc cgctggcctt gaagccgggg atagcggtcg
     3901 gctccagctt gctggtgacc ttggtcttgg ggttggtgaa gttcttggtc tggcgctcac
     3961 gcatctcgaa acggccaaag cctgcgatgg tgaccttgtc gccgctcttg agcacatcgc
     4021 cgatggtgcc gaaaatgccg tccataacct tctccacttc agcggcggtc atgttggtgt
     4081 cattggcaac cttggtaatt aactgagatc tcgtcatgat atttttaact cctatcctta
     4141 taggcacaca tagcctttat tattacgttg tttattatag agaaaattgt gcgcagaatc
     4201 aagagttttt tcgcaaaaag gcggtttttc ggcaaaaatc ataaaatttg cgagcggaat
     4261 ctggcaaaaa gtgacatatg gcaaaaaatg cacgttctgc gctggaatat ccgagcggcg
     4321 cagcgcggta caagcccatc tgcgcggcgt cggggtagct ggcgggcgca ccgcctaccg
     4381 gggcgcaaag gcgggtacct cggcaaaggc gcagccgctt ttggacggta tcatgtcgat
     4441 cagcgctttg acctcgcgta caaagtatca cgaggcatag cggacctcct tacagattca
     4501 gcgggtagac tgcggcggta aagttggcgg gccgcagtgt gacctgaccc ccggcggggt
     4561 agtaccgggt ggacagaacg gccgcgccat cgttgaggaa aatttccagc gaggacgcat
     4621 cccccgccac ccgtagacga cggcagggcg catccagcac cacacagcgg ctcgtgcggc
     4681 cgccggacat ggcggcgtcg gtaaaggtca ggcagcaggt gcggtcggcg tcactgtaag
     4741 ccagcgtcag cccgccgtca aagcccagag agaagtcccc gccaggggcg gcaaccaggt
     4801 cgaagcaggc atccacggcc tgctctgcac cgtctgtggc ggtcacagcg gccccgcggc
     4861 gggcggccag ctcggacacc gggttttgca ggatgtgccc ggcattgtcc cgggtcagca
     4921 cccgggaaat cgtcatgcag tgctgccagc cgtaggccac ggtggcgacg ttggtagccc
     4981 tgttcgtcca gcacgcggct gatgccctcc accatgcggg cgcagctctc actgctgagg
     5041 gtgggcataa tgacgccgat ctgcagggta cggcgggtgt gcatcatcta cgcctgcata
     5101 ctggggcggt agccctataa tggcaatcca caacaaaacg cctgcaccct ttttacaggt
     5161 acaggcgttc atgctatttg ttatttccca tttgccgctt cgcggtgcaa attcgcgata
     5221 tccaccagct ccatctggct gatatcgtgg tcgccgcgca gggcgtccac cagcacgctg
     5281 gcggtgtcca tggccgtgat ggtcgggatg gacagctcgg tggccttgcg gcggaacttg
     5341 accgagtcgc ggtgcgggtc gcggccatgg gggctggtgg agatgatgta atccaccagg
     5401 ccgctttcca gcagatccag cacgttgggg cgtgcgccgc tcatctggcg gacgacgctg
     5461 cagggtatca tctcgccgtt caagtacttg gctgtgccgg gtgtgccgta aatcttatag
     5521 ccgtagtcgt gcagcttcca ggccagcttg gcagcttcag ctttgtcgct gtccttgacc
     5581 gagataatga cgcaggagcc ggggccgggc accttgaact ggtagccagc cgccaccagg
     5641 cccttcatca tggcctcgtg gaaggtgggg gcgatgccca gcacctcacc ggtggacttc
     5701 atctcggggc ccagcatcgt gtcaacgccg tgcagcttca ggaaactgta cacaggcacc
     5761 ttgacggcgt agtaggggct ctgcccattg cgccacagac cggtgccgta gcccatatcc
     5821 ttgagctttt cgcccagcgt gcagcgcacg gccagatcaa ccatcggtac gccggtgact
     5881 ttggagatat agggcacggt gcgggaggaa cgcgggttga cctcgatgac gtagacgcgg
     5941 tcgttctgca cagcgtactg cacgttgacc aggcccacga ccttcagctc gcgggcaaag
     6001 cggccggtgt agtcgaccag cgtgtcaatg accttctggc tgaaggtcgt gggcgggtag
     6061 acacagatgg agtcgccgga gtgaacaccg gtacgctcga cctgctgcat gatgccgggc
     6121 acgaggtaat ccgtgccgtc gcagatggcg tcgacctcgc actcggtacc caggatgtac
     6181 ttgtccatca ggaccggatg gctcatgtca acgtgcttcg tgatgacgcc catgtactcg
     6241 ataacgttgc tggaattgta ggccacgatc atgttctgac cgcccagcac gtaggaggga
     6301 cg
//
LOCUS       DBMDSQ010000093         6237 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_3192_length_6237_cov_1.938070, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000093 DBMDSQ010000000
VERSION     DBMDSQ010000093.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 6237)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6237)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6237
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3192_length_6237_cov_1.938070"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(252..1448)
                     /locus_tag="ACZPAX_04765"
     CDS             complement(252..1448)
                     /locus_tag="ACZPAX_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HIR1113243.1"
                     /translation="MYSIFRDLAIIILSAKFFALVARKCKAPQVVGEIIAGLLIGPCL
                     LNLVQTSDSISIFAEIGVVLLMFTTGLGTNLKELMRAGPIATLIACVGVFVPLVGGTL
                     LYGAFYGFAAVGSPEFYRALFIGTIMTATSVSITVATLQELGHLKSFLGTTIVSAAVI
                     DDVIGIIVLTCVLGASSGEGTGIGGVLIQTVLFFLVAIGVGVIIHYAMKWLDARNPHT
                     QRITVVSLAFCFAMAYIAEQYFGIADITGAYIAGIVLCTMNDASYVERRVDISNYVIF
                     APVFFASIGLKTDISGLTPEIFLFCVCFVIVALATKIIGCGLAAKICRFNWGDALKVG
                     VGMMTRGEVALIVAQKGLEVGVVDPVYFTAVILLIVVSSVATPLALKALFTKMPPVPH
                     PSQAKA"
     gene            1767..1855
                     /locus_tag="ACZPAX_04770"
     tRNA            1767..1855
                     /locus_tag="ACZPAX_04770"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1802..1804,aa:Ser,seq:gga)
     gene            2029..2406
                     /locus_tag="ACZPAX_04775"
     CDS             2029..2406
                     /locus_tag="ACZPAX_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014184043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HIR1113244.1"
                     /translation="MLNHEPLRLAEGEYRFACLVWDHEPLPSGQLVTLAADALGWKKS
                     TTYTVLKKLCERGVLQNADGQVTSLIKKEDVQQAESAAVVDKTFGGSLPRFVAAFLNT
                     QPISDAEAAEIRALLDAAHREEG"
     gene            2409..4316
                     /locus_tag="ACZPAX_04780"
     CDS             2409..4316
                     /locus_tag="ACZPAX_04780"
                     /inference="COORDINATES: protein motif:HMM:NF017390.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HIR1113245.1"
                     /translation="MCSTVFAFLLLSARGGAAALAAWAVCKLLRRAHAPSRLLCWLWL
                     AVGLRFVLPWGIPLSLPRPQNTQLAAAADTVQELGELPLVAPPPAVSAPAAALPWYAR
                     LTPWHALAAVWAVGVAVLAVRGIVGYIKLKCHVALACKTSDGCYSGACVTAPFTLGIL
                     RPRVYLPDDLQGTARQAVLLHEQTHIRRRDPLTKPLFYAVACLHWFNPLVWLAFCTFE
                     RDMEAACDEAAVRGRPLPERNAYCESLLHFAVQGRSIPGSLAFGQGSVKERIVHLLHY
                     RRLGAGALAVCAAVVGLSVTACMVRPQVEDAPATAAPPATAETAEPTPTPAPTSVPAV
                     AAAALPTLDNAANSPRFICPVKYKYISRFATNGHRGDDLCAAEGTEIYAAADGVVLAA
                     QEHYSWGNFVEIDHGADANGLRWSTLYAHMQSCAVQVGQTVTAGQVIGYVGSTGNATG
                     NACHFEMQVNGTLVEPRYFTAYGGSDAAELTQEKADEILAEAVRRAASDQVTAADGAA
                     LSGVDLFTLPVAPPPQVSGYDPENGHPGIDFAAEEGAEVYAVAGGIVTAADYDVEKGN
                     YVVLDHGGGLETEYQHMKSLLVSAGQSVVQGQVLGYVGSTGNSTGPHLHFEARQDGAP
                     ADLTGTALLAE"
     gene            4321..5550
                     /locus_tag="ACZPAX_04785"
     CDS             4321..5550
                     /locus_tag="ACZPAX_04785"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC family protein"
                     /protein_id="HIR1113246.1"
                     /translation="MKYRHFPAAVLAAGLLFCTLFSGFDAYADDKKGELTQNQAEARQ
                     EYEAEQAALNELKSAQAQTENEVAALTGQAAELAGQITDVTAAVQAAQTELDVRRAAA
                     EAAGTSLTAKQTEYDARLALCKEQLRAMQRLDGGGALWLLAQAKSLYQVLTFDTVLRQ
                     MSAKNSAVLAELDAEAAALEQARAEAETAAQQAADAKAALDAQQAALQATQCELETAL
                     LDANSALTAQQAAAQAQAAVTDAAKKAYEAATAALDAYVRAQSQRYTTADLHLTSLAF
                     RCPLDSYGRITTQFGEPDPWGIPHRGTDFAAPGGTPIYAIADGVVSAARTMNSYGNCV
                     QVSHGTANDGHCYDSLYAHMSSIVVAQGDTVQKGDLLGYVGNTGNVYGAGGGYHLHLE
                     LRVDGGRVNPLGYVPTL"
     gene            5565..>6237
                     /locus_tag="ACZPAX_04790"
     CDS             5565..>6237
                     /locus_tag="ACZPAX_04790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113247.1"
                     /translation="MTFGDVLYFVGFHVEYTAVRVGRGTAFWLRELARALVQLLAVVL
                     RPPVRAAADFRAALIHPHRLVGLLLPLAAGAALVFYVRTTLAQPFVLRVEVGGQTVGY
                     LPGEQAFEAARADVQARISGAADWDIQPEYTLVWGAGVQTMTERETADAILRAAGGDI
                     TEGTAVYIDGTLRYITTEGDHLRRCLYAVRQLWQAEGTQTDFVHPLRLVDGIYPAVAV
                     TPYGTL"
BASE COUNT         1184 a         1928 c         1975 g         1150 t
ORIGIN      
        1 tggagctgct gatcaaatgg gtggacaatc atctgcataa tgcagaataa aaaatgaaaa
       61 ggcaggcgca aagcagcgtc tgcctttttt agtttctctt tgatgggagt tcccaccgca
      121 gcgggtgagg ggtgggccgc agcctcacgg cataaacagt cagcttccgg ccgcggctcc
      181 ccttctggcg ctatgcgcca cctcctccgt atcggaggag gctgtcctca aaggggaggc
      241 ttttactcga attacgcctt tgcctggctc ggatgcggga cgggcggcat ttttgtaaac
      301 agggctttca gcgccagcgg tgtggcgacg ctggacacaa cgatcagcag gatgaccgcc
      361 gtaaagtaga ccgggtcaac gactccgact tccaggccct tctgggcaac aatcagcgcg
      421 acctcaccgc gggtcatcat gccgacaccg accttgagag catcgcccca gttaaagcgg
      481 cagattttgg ccgccaggcc gcagccgata atcttggttg ccagcgccac aatgacaaag
      541 cagacgcaga acaggaagat ttccggcgta aggccgctga tatcggtttt caggccgatg
      601 gacgcaaaga acaccggtgc aaagatgaca tagttgctga tgtccacgcg gcgctcgacg
      661 taggaggcgt cgttcatcgt gcacaggacg atgcccgcaa tgtacgcgcc ggtgatatcg
      721 gcaatgccga agtactgctc ggcaatgtag gccatagcga agcagaacgc caggctgaca
      781 acggtgatgc gctgggtgtg ggggttgcgg gcgtccagcc acttcatggc gtagtggatg
      841 atgacgccca caccgatggc gacaaggaag aacagcacag tctggatcag cacgccgccg
      901 atgccggtgc cctcgccgga gctggcaccc agcacgcagg tcaatacgat gattccgatg
      961 acgtcatcaa tgaccgccgc gctgacgatg gtcgtgccga ggaagctctt caaatgacca
     1021 agctcctgca gcgttgccac tgtgatggaa acgctggtgg ccgtcatgat ggtgccgatg
     1081 aacagcgcac ggtaaaactc cgggctgccc actgccgcaa agccgtagaa cgcgccatac
     1141 agcagcgtgc cgcccaccag cggcacaaac acgcccacgc aggcgatcag cgtagcgatg
     1201 gggccggcac gcatcagctc tttcaggttc gtgcccaggc cggtggtgaa catcagcagc
     1261 acgacgccga tttcggcgaa gatggaaatg gaatcgctgg tctgtaccaa gttcagcagg
     1321 cacggcccga tgagcagacc ggcgatgatc tcgcccacga cctgcggggc cttgcacttg
     1381 cgggcgacca gcgcaaagaa tttggcgctc agaataatga tcgccaggtc acgaaaaatg
     1441 gaatacatga aatatcctct ccttttgtcc gggtgaaatc gtgcacccac agaaattggg
     1501 aagtcgtaca tccccgatgc acgcattttt gatctgcagg gggggcgcaa tgcccggaag
     1561 atgtttcaca cctatatagt tttagcacct tgcacggcgg tttgcaagag gataaaacgt
     1621 acaaaatatg cagttcgagc gcatattttg cgtgcatttc gcccatcggg aaaattttcg
     1681 agaattttct gcaatttttg cttgacattt gccgcaccga ttgctataat agctcatgca
     1741 gtcaggcgct gcacaactga atatgtggaa agatggctga gctggtctaa ggcgcacgac
     1801 tggaaatcgt gtagggcccc taaagccctc aagggttcga atccctttct ttccgcttat
     1861 gaaccgccct cgtgagtttt catgagggcg gtttttctgt tgcccctatg ccccaagttc
     1921 cctgcctcat atctgcggcg gggaattttt attgacattt ttggtctatt gtagtagact
     1981 ataagcaagg acaggtctat tgcaataaac caggaaggag cttttcctat gctaaaccat
     2041 gaaccgctgc gccttgccga aggtgaatac cgttttgcct gccttgtgtg ggaccatgag
     2101 ccgctgccca gcgggcagct ggtgacgctg gccgccgacg cgctgggctg gaaaaagagc
     2161 acgacctata ccgtgctgaa aaagctctgc gaacggggcg ttctgcaaaa cgccgacggc
     2221 caggtgacga gccttattaa aaaggaagat gtccagcagg ccgagagtgc cgccgtggtg
     2281 gataagacct ttggcggcag cctgccgcgc tttgtggctg cctttttgaa cacccagccc
     2341 atcagcgacg ccgaggccgc cgagattcgc gcactgctcg acgccgccca ccgcgaggag
     2401 ggatagttat gtgcagtacc gtgttcgcgt ttttgctgct cagcgcccgg ggcggcgctg
     2461 ccgcgctggc ggcctgggcc gtctgtaagc tgctgcgccg cgcccacgcg cccagccgcc
     2521 ttttgtgctg gctgtggctt gccgtggggc tgcggttcgt gctgccctgg ggcatcccac
     2581 tttccctgcc ccgcccgcag aacacccagc tggccgcagc tgctgacacc gtgcaggagt
     2641 taggcgaact gccgctggtc gcgcccccgc ccgctgtctc cgcgcccgcc gcggcgctgc
     2701 cgtggtacgc ccggctgacg ccctggcacg cgctggccgc cgtgtgggcg gttggcgtag
     2761 cggtgctggc cgtgcgcggt atcgttgggt acataaaatt gaaatgccat gtggcgctgg
     2821 cctgcaaaac atcggacggc tgctacagcg gggcctgcgt gactgcgccg tttaccttag
     2881 gtattctgcg cccgcgcgtc tacctgcccg acgatttgca gggaaccgcc cggcaggccg
     2941 tgctgctgca cgagcagacc cacatccgcc gccgcgaccc gctgaccaag ccgctgttct
     3001 acgccgtggc ctgcctgcac tggttcaacc cgctggtctg gctggcgttt tgcacctttg
     3061 agcgcgacat ggaggcggcc tgcgacgagg ccgccgtgcg gggccgtccc ctgcccgagc
     3121 gcaacgccta ctgtgagagc cttctgcatt ttgccgtgca gggccgcagc ataccgggca
     3181 gcctggcttt cggccagggc agcgtgaagg agcgcattgt ccatttgctg cattaccgcc
     3241 gtttgggtgc cggggcattg gccgtctgcg ccgccgtggt gggtttgagc gtaacggcct
     3301 gcatggtgcg cccgcaggtc gaggatgccc ccgccaccgc tgcgccgccc gccaccgccg
     3361 agacggcaga gccgaccccc acacccgcac cgaccagcgt gcccgctgtg gctgccgctg
     3421 ccctgccgac attggataac gctgcgaaca gcccgcgctt tatctgccct gtcaagtaca
     3481 aatatatttc ccgctttgcc acgaacggcc accggggcga cgatctatgc gctgccgaag
     3541 gcaccgaaat ttacgccgcc gcggacggtg ttgtgctggc cgcgcaggag cattacagct
     3601 ggggcaattt tgtcgagatc gaccacggcg ccgacgccaa cggtctgcgc tggtccacgc
     3661 tgtacgccca catgcagtcc tgcgccgtgc aggtcgggca gaccgtgacc gccggtcagg
     3721 tcatcgggta cgtgggcagc accggcaatg ctaccggcaa cgcctgccat tttgagatgc
     3781 aggtcaatgg cacgctggtg gagccgcggt attttaccgc ctacggcggc agcgatgccg
     3841 ccgaactgac acaggaaaag gcggatgaaa tcttggccga ggccgtgcgc cgtgccgctt
     3901 ccgaccaggt gactgccgcc gatggagccg ccctttccgg cgtggatctc tttaccctgc
     3961 cggtcgcgcc cccgccgcag gtcagcggct atgaccccga aaacggccac cccggcattg
     4021 actttgccgc cgaggaaggc gcggaggttt acgccgttgc cggtggcatc gtgaccgccg
     4081 ccgactacga tgttgaaaag ggcaactatg tcgtgcttga ccacggcggc gggctggaaa
     4141 cggagtatca gcacatgaag tccctgctcg tttctgccgg gcagtccgtg gtccaaggcc
     4201 aggtcttggg atatgtgggc agcaccggca attccaccgg gcctcacctg cactttgagg
     4261 cacggcagga cggcgcgccc gcagacctga ccgggactgc cctattagcg gagtaacaag
     4321 atgaaataca ggcacttccc cgccgctgta ttggcggcgg ggctgctttt ttgtaccctt
     4381 ttttcgggtt ttgacgccta tgcggatgat aaaaagggcg aattaacgca aaaccaggcc
     4441 gaagcgcggc aggaatatga agccgaacag gcagccttaa acgaactaaa aagcgcccag
     4501 gcgcagaccg agaacgaggt tgccgcgctc accgggcagg cggcggaact ggcggggcaa
     4561 atcaccgacg tgactgccgc cgtgcaggcc gcccagaccg aattggatgt tcgccgggct
     4621 gccgcagaag ccgccggcac gtcgctgact gccaagcaga ctgaatatga cgcgcggctc
     4681 gccttgtgca aggagcagct gcgggccatg cagcggctgg acggcggcgg ggcgctgtgg
     4741 ctgttggcgc aggcgaaaag cctgtaccag gtgctgacct ttgacacggt gctgcggcag
     4801 atgagcgcca aaaacagcgc cgtactggca gagctggacg ccgaggccgc cgcgcttgaa
     4861 caggcccgcg ccgaagccga gaccgccgcc caacaggctg ccgacgcgaa agcggcgctg
     4921 gatgcccagc aggccgcgct gcaagccacg cagtgcgagt tggagacggc gcttttggac
     4981 gcgaacagcg ccctgaccgc gcagcaggcc gccgcccagg cccaggccgc cgtgaccgac
     5041 gccgcgaaaa aagcctacga ggctgccacc gccgcgctgg acgcctacgt gcgggcgcag
     5101 agccagcgct acaccacggc agatttacac ctgacgagcc tagcctttcg ctgcccgctg
     5161 gacagctatg ggcgcatcac gacccagttc ggcgagcctg acccctgggg tatcccccac
     5221 cggggcaccg attttgctgc gccgggcggc acaccgattt acgccattgc cgacggcgtt
     5281 gtcagcgccg cgcggaccat gaacagttac ggcaactgtg tacaggtcag ccacggcact
     5341 gccaacgacg gccattgcta tgacagcctg tacgcccaca tgagcagcat cgtcgtagcc
     5401 cagggtgaca ccgtgcagaa gggtgatctg ctggggtacg tgggcaatac cggcaatgtt
     5461 tacggcgcag gcggcgggta tcacctgcat ctggaattgc gggtggacgg cggcagagtg
     5521 aatccgttag ggtatgtgcc gacattgtag ggaggctgtc tgctttgact ttcggggatg
     5581 ttttatattt tgtcggcttc catgtcgaat acaccgccgt gcgggtcggg cgcggaacgg
     5641 cattttggct gcgggagctg gcccgggcgc tggtgcagct gttggccgtg gtgctgcggc
     5701 ccccggtgcg ggcggcggca gatttccgtg cagcgctcat ccacccgcac cgccttgtcg
     5761 gcctgctgct gccgctggcg gcgggggcgg cgctggtttt ctacgtgcgc accacactgg
     5821 cacagccttt tgtgcttcgg gtcgaggttg gcggccaaac cgtcggctat ctgcccggtg
     5881 agcaggcctt tgaggccgcc cgcgccgacg tgcaggcccg catcagcggc gcggcggatt
     5941 gggatatcca gcccgagtac acccttgtct ggggtgcggg cgtgcagacg atgaccgaac
     6001 gcgagaccgc cgacgcgatt ttgcgagcgg cgggcggcga catcaccgaa ggcaccgccg
     6061 tttacatcga cggcacgctg cggtacatca ccaccgaggg cgaccacctg cgccgctgcc
     6121 tgtacgccgt gcgccagctt tggcaggccg agggtacgca gacagacttt gtccacccgc
     6181 tgcgcctggt agacggcatc taccctgctg tcgccgtcac gccctacggc acattac
//
LOCUS       DBMDSQ010000094         6227 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_3203_length_6227_cov_1.670584, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000094 DBMDSQ010000000
VERSION     DBMDSQ010000094.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 6227)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6227)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6227
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3203_length_6227_cov_1.670584"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(15..1232)
                     /locus_tag="ACZPAX_04795"
     CDS             complement(15..1232)
                     /locus_tag="ACZPAX_04795"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005929310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016628 - oxidoreductase activity, acting
                     on the CH-CH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BaiN/RdsA family NAD(P)/FAD-dependent
                     oxidoreductase"
                     /protein_id="HIR1113248.1"
                     /translation="MTDLLVIGGGAAGLMAAGAACARSLTVTVLEHNPKGPGQKLLIT
                     GKGRCNVTSDSDVREFLPYVRHNGRFLYSSLYAFPPSAAMELFESLGVPLKTERGRRV
                     FPQSDRAADVLAALRDYARDAEFVRGSAKKLLIEDGVCKGTVTDRGETLRAENTLIAT
                     GGISYPATGSDGSGYKLARQAGHSIVPPQPSLCSLVSPDPACRRMMGLALRNVTLTLL
                     CDGKPLFTEQGEALFPHFGLSGPLVLSASTYIEDITKHRYICEFDLKPALDEKTLYDR
                     LTRDFAEQGSHSAQGALAKLLPNSMRPVAVEKWGVDPATRAAQITREQKQALVDLCKH
                     WQVPVSALGDKEHAVITAGGVDVREVDPKTMASKLCEGLYFAGEVLDVDARTGGYNLQ
                     IAWATAQAAVRAL"
     gene            complement(1229..2932)
                     /locus_tag="ACZPAX_04800"
     CDS             complement(1229..2932)
                     /locus_tag="ACZPAX_04800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113249.1"
                     /translation="MKNLHKKQLRLSRRGFWLVCALLVCLRLTLTSFQQAYTWVGGAP
                     LDDELMFRAAQNITAGEWLGAYDYLTLSKAMFFPVWLALLHALHLPYLVAGATLWCGA
                     ALLAAFAFSPLWRKKQPEQGRVLTLALFAALAFLPSSWAAYTLRVYRDNIFPALCLYF
                     FAGMAGMALRAVFEKDKPLWPWLAAAGAGLACGYLDREDAGLFLLPFAAAATVIVAVV
                     LVGQRRWRAFAAQVIPYAMLGVGVLTFCTLNYTHYGVFALSDFSEGSFAAAMGAMMRV
                     DTDSDKPYLSVPADAREKIYEAVPELKPVAYWLEEDAQMENDFRDPGLDDYRAGSFYW
                     AIRRAAQYEGIYADAQTAANYWQTVADKINAACDAGTLPSRTGKRVATSQPITAAYVP
                     ATLAETWNGFWHVLGFRDCAPYEALRSIGTDDDIAAWSGYLHCGFNAAANAGEDTPYY
                     SPYQRAIFAVMQGWTWVYSVMLTVCVFCAVVFQLMKFLSCLRKKCAAETVVPWLLLFG
                     IFGIALLRCAMIAFVEVSSFGIGTSTMYLAPVHPLLVLYVFAGLFLYGRPISVKRKDN
                     A"
     gene            complement(2936..3847)
                     /locus_tag="ACZPAX_04805"
     CDS             complement(2936..3847)
                     /locus_tag="ACZPAX_04805"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR1113250.1"
                     /translation="MNTKEKNFVSAVVYLHNDGARAVEFCRTAAAELDAHFAQYELVV
                     VDDACTDDTVAQLRVWGKEQAAPLTILHMSTYHGLENAMNAGLDAAIGDYVYEFDSTE
                     LCYPAELIFKAYQTALNGSDIVSVCPRTVRGSSGLFYKVFNAHSQSAYRLRTDAFRLV
                     TRRAINRVHASSAHLPYRKAAYAASGLKMTDLEFDGQLTVKSKGRFNLALDSLALYTN
                     AGFKASMTVTLCMLALALAELVYTLVVFITGHPVEGWTTTMFVITVGFSGLFAVLTIV
                     VKYLSLLVDLVFKQQKYLVESIEKLQK"
     gene            complement(3844..4962)
                     /locus_tag="ACZPAX_04810"
     CDS             complement(3844..4962)
                     /locus_tag="ACZPAX_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR1113251.1"
                     /translation="MTYDKIILGAGLYGLYAAQKCGAAGQRVLVLERDPAPFMRATYI
                     NQARVHMGYHYPRSYSTAIKSAHYFERFCADYGFCLHTEFDQVYATSAHFSWTNAAEF
                     RRFCAAAGIRCDDVAPERYFNPGLCDGAFLTKEYTYDAQVLKRWFLEKLAALPNVEIL
                     YSHKPDKIEKAGGAWQVTAGDTTAEAPYLLNATYAGVNDVHALLGLPPFGIKYEKCEI
                     ILCTVEDALKHTGITVMDGPFFSLMPFGQTGLHSLTSVTFTPHETSYDSVATFPCQAE
                     SGGCCTPGSLYNCNECPAKPQSAWPYMSQLARKYLKEEYGFAYHSSLFSMKPILKASE
                     IDDSRPTVVRVMSDEPKLVSVLSGKINTVYDLDEVLEV"
     gene            complement(4964..>6227)
                     /locus_tag="ACZPAX_04815"
     CDS             complement(4964..>6227)
                     /locus_tag="ACZPAX_04815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113252.1"
                     /translation="YTHETAIVYRDSQDYLAYDPIRALQGSAVNGRAPVYGTILYLIR
                     IIVGEWHQLHAATFLQELVGIAVLPLFSATLRRVGVGTRVRAAVLLLYGFCPAVYGWD
                     NVVLTESLSLSATVVFFFLIVRYIQERRFWDGAGALLVTLWLIFLRPQFLTYLAMLMV
                     FFVWRMFYADKKEKKDLGILLAWGAVAALGIIVYCASFSNQFGIFSLTDAGPRQDLRV
                     CVDRGYYKDFADEDARQIVEDAIAEDTGIDGYAVGKLLSVYGNAKGQKLAKDFFRTNA
                     VRYIKDTCNVMWNYRAIELSAYGLELRYGDDRVYNLCLAIAQEGELLANPLEHWLTLY
                     AAYILSLLWGVVMVIAWVQQKRPPWLHMALFALLMCTTFLTFFITCGEYMRTMITVVP
                     YMFICMGMAVQRLIVPHGLVSVCPKGEK"
BASE COUNT         1273 a         1924 c         1891 g         1139 t
ORIGIN      
        1 aggaaggctt ggggttacag cgcccgcacc gccgcttggg ccgtcgccca agcgatttgc
       61 aggttatacc cgccggtgcg ggcgtccaca tcaaggacct caccggcaaa gtacagcccc
      121 tcgcagagct tgcttgccat cgtcttggga tcgacctcgc gcacatccac gccgcccgcc
      181 gtgataacgg cgtgttcctt gtcgcccagc gccgacaccg gcacctgcca gtgcttgcac
      241 aggtccacaa gggcctgctt ctgctcccgc gtaatctgcg cggctctggt ggcagggtca
      301 acgccccact tttccacagc taccgggcgc atggaattgg gcagcagctt agccagtgcg
      361 ccctgcgcgc tgtggctgcc ctgctcggca aagtcgcggg tcaggcggtc atagagcgtt
      421 ttctcgtcca gcgcaggctt taggtcaaac tcacagatat agcggtgctt cgtgatgtcc
      481 tcgatgtagg tactggccga gagcaccagc ggcccggaca ggccgaagtg ggggaacagt
      541 gcctcgccct gctcggtaaa cagcggcttg ccgtcgcaga gcagtgtcaa cgtgacgttg
      601 cgcagggcca ggcccatcat gcggcggcag gccgggtcgg ggctgaccag gctgcacagg
      661 ctgggctggg gcggcacaat gctgtgcccc gcctgacgcg ccagcttgta gccgctgccg
      721 tcgctgccgg tggccgggta gctgatgccg ccggtggcga tcagcgtgtt ctcggcccgc
      781 agtgtctcgc cccggtcggt gacggtgccc ttgcagacgc cgtcctcgat cagcagcttt
      841 ttcgcgctgc cgcgcacaaa ttcagcgtcc cgggcatagt cccgcagggc ggccagcacg
      901 tcggcggcgc ggtcgctctg ggggaacacc cgccgcccgc gctcggtttt gagcggcacg
      961 ccgagggatt caaacagttc catcgccgct gacggcggaa aggcgtagag gctcgagtac
     1021 aggaaccgcc cgttgtggcg cacatagggt aaaaactccc gcacatcgct gtcacttgtg
     1081 acgttgcagc gtcctttccc ggtgatgagc agcttttggc cggggccttt ggggttgtgc
     1141 tccagtacgg tcacggtcag gctccgggcg caggccgccc cggctgccat caggcccgcc
     1201 gcaccgccgc cgatgaccaa taagtccgtc atgcgttgtc ctttctttta acagatatag
     1261 gccgtccata caaaaacagc ccggcaaata cgtagagaac cagcagcggg tgtaccggag
     1321 ccaggtacat ggtgctggtg ccgatgccga agctggacac ctcgacaaaa gcgatcatgg
     1381 cgcagcgcag cagcgcaata ccgaagatgc cgaacagcag cagccagggc acaacggtct
     1441 ccgccgcgca tttttttcgc agacacgata aaaacttcat aagctggaac accaccgcgc
     1501 agaacacgca aaccgtcagc ataacgctgt acacccaggt ccacccctgc ataacagcga
     1561 aaatcgcacg ctggtagggg ctgtaatagg gcgtgtcctc ccccgcgttg gcggcagcgt
     1621 tgaagccgca gtgcaggtag ccggaccatg ccgcaatgtc gtcgtcggtt ccaatcgacc
     1681 gcagcgcctc gtagggcgca cagtcgcgga agcccagcac gtgccagaag ccgttccagg
     1741 tctcggccag cgtggcgggc acgtaggccg ccgtgatggg ttggctggtg gcgacccgtt
     1801 tgccggtgcg gctgggcagg gtgcccgcgt cgcaggcggc gttgatcttg tccgccacgg
     1861 tctgccaata gttggctgca gtctgggcgt cggcgtagat gccctcatac tgggcggcgc
     1921 ggcggatggc ccaatagaag ctgcccgcgc ggtagtcgtc aaggcctggg tcgcggaagt
     1981 cgttctccat ctgggcatcc tcttccaacc agtaggcgac gggtttcagt tccggcacag
     2041 cttcgtagat tttttcgcgt gcgtcagcgg gcacagacag gtacggtttg tcgctgtccg
     2101 tgtcaacgcg catcatcgcg cccatggcgg cggcaaacga gccctcggag aaatccgaca
     2161 gggcaaacac accgtagtgg gtgtagttga gggtgcagaa ggtcagcaca ccgacgccga
     2221 gcatggcgta ggggatgacc tgcgcggcaa aggctctcca gcggcgctga cccaccagca
     2281 ccaccgccac aatgacggta gccgccgcgg caaagggcag caggaacagc ccggcgtcct
     2341 cgcggtcgag gtagccgcag gccaggcccg cgcccgccgc tgccagccag ggccagagcg
     2401 gcttgtcttt ttcaaagacg gcccgcagtg ccatcccggc catgcccgca aaaaagtaca
     2461 ggcagagtgc cgggaagatg ttgtcgcggt aaacgcgcag cgtgtaggcc gcccaggacg
     2521 acggcagaaa cgccagcgcc gcaaacagcg ccagcgtcag cacacggccc tgctcgggct
     2581 gttttttgcg ccacagcggc gaaaatgcaa acgctgccag cagcgccgcg ccgcaccaga
     2641 gcgtcgcccc cgcgaccaga tacggcaggt gcagtgcgtg cagcagggcc agccacaccg
     2701 ggaagaacat ggccttggac agcgtcaggt agtcatacgc gcccagccac tcccccgccg
     2761 tgatattctg cgccgcgcgg aacatcagtt catcgtccag cggggcgccg cccacccagg
     2821 tgtacgcctg ctgaaagctc gtcagcgtca ggcgcaggca gaccagcagg gcgcagacca
     2881 gccagaagcc ccggcgggac aggcgaagtt gctttttgtg taaattcttc atggatcact
     2941 tttgcagctt ttcgatgctc tccactaaat atttctgctg cttgaacacc agatccacca
     3001 gcagcgacag gtacttgacc acgatggtca gcaccgcaaa cagcccggaa aatcccaccg
     3061 tgatgacgaa catcgtggtg gtccagccct cgaccgggtg gccggtgatg aacaccacca
     3121 gcgtgtagac cagctcggcc agggccagcg ccagcatgca gagggtgacc gtcatgctgg
     3181 ccttgaaccc agcgttggtg tacagcgcca ggctgtccag cgccaggttg aaccgaccct
     3241 tgctcttgac ggtcagctgg ccgtcaaatt ccagatcggt cattttcagg cccgacgctg
     3301 cgtaggccgc cttgcggtag ggcaggtggg cactggaagc gtgtacgcgg ttgatggcgc
     3361 ggcgcgtgac cagccggaac gcatcagtgc gcagccggta ggcactttgg gaatgagcgt
     3421 tgaacacctt gtaaaacagg ccgctgctgc cccgcacggt gcgcgggcag accgagacga
     3481 tgtcgctgcc gttcagcgcg gtctggtatg ccttgaaaat cagctccgcc gggtagcaaa
     3541 gctcggtgga atcgaactca tacacatagt cgccgatggc agcgtcaaga cccgcgttca
     3601 tggcgttttc caggccgtgg taggtgctca tgtgcaaaat cgtcagcgga gcggcctgct
     3661 ctttgcccca gacgcgcagc tgggccacgg tgtcgtcggt gcaggcgtca tccacgacga
     3721 ccagctcata ctgggcgaag tgggcgtcca gctcggcggc ggcggtgcgg cagaactcca
     3781 ccgcgcgggc accgtcgttg tgcaggtaga caaccgccga gacaaagttt ttttcttttg
     3841 tgttcatact tccagcacct cgtccaggtc atatacggtg ttgatcttgc cgctcagcac
     3901 gctgacgagc ttcggctcat cgctcatgac gcgcacgacc gtcgggcgcg agtcgtcaat
     3961 ttcgcttgcc ttcaaaatcg gtttcatgct gaacaggctg ctgtggtagg caaaaccgta
     4021 ttcttccttt aaatatttgc gggccagctg gctcatgtag ggccaggcgc tttgcggctt
     4081 ggccgggcac tcgttacagt tgtacaggct gcccggggtg cagcaaccgc cgctttctgc
     4141 ctggcagggg aaagtcgcga cgctgtcata gctcgtctca tgcggggtga aggtcacgct
     4201 ggtcaggctg tgtaggccgg tctggccgaa gggcatcagg ctgaaaaacg gcccatccat
     4261 gaccgtgatg ccggtgtgct ttaaggcatc ctccaccgta cacaagatga tctcgcattt
     4321 ttcgtacttg atgccgaagg gcggcagacc cagcagggcg tgcacgtcgt tgacgcctgc
     4381 ataggtagcg ttgagcaggt agggcgcttc ggcggtggtg tcccccgccg tgacctgcca
     4441 cgcgccaccg gctttctcga tcttgtcggg cttgtggctg tacaggattt cgacattcgg
     4501 cagggcggcc agcttctcca ggaaccagcg cttgagcacc tgggcgtcgt aggtgtattc
     4561 tttcgtcagg aatgcgccgt cgcaaaggcc ggggttgaag taccgctcgg gcgcaacatc
     4621 gtcacagcgg atgcccgctg ccgcacagaa acggcggaat tcggcggcgt tcgtccagga
     4681 gaaatgggcg ctggtggcgt agacctggtc aaactccgtg tgcaggcaga agccgtaatc
     4741 cgcacagaaa cgttcaaaat agtgggccga cttgatggcc gtggagtagc tgcgcgggta
     4801 gtggtagccc atatggacgc gggcctggtt gatgtaggtt gcccgcataa agggcgcggg
     4861 gtcacgctcc agcacaagga cgcgctggcc cgccgcgccg catttctgcg ccgcatacag
     4921 cccatacagc cccgcaccga ggatgatttt atcgtaggtc aatttatttt tctcctttgg
     4981 ggcacacact tacaagccca tgcggcacaa taagccgctg caccgccata cccatacaaa
     5041 tgaacatata cggcacaacg gtaatcatcg tccgcatata ttcgccgcag gtgataaaga
     5101 aagtcaagaa agtcgtacac atcagcaggg caaacaaggc catatgcagc caaggcgggc
     5161 gtttttgctg cacccatgca atgaccatca ccacgcccca gagcagcgag agaatgtacg
     5221 cagcatacag tgtcagccaa tgttccagcg ggttagccag taactcaccc tcctgcgcga
     5281 tggcaagaca caggttatac acacggtcat cgccgtagcg cagttccaat ccataagctg
     5341 acagctcgat cgcgcgatag ttccacatta cgttgcaggt atccttgata tagcgcacgg
     5401 catttgtgcg gaagaaatcc tttgccagct tttgtccctt ggcgttgccg tatacggaca
     5461 gcaatttacc gactgcgtag ccgtctatgc cggtgtcctc ggctatggcg tcctccacga
     5521 tttgtcgggc atcctcgtcc gcaaaatctt tgtaatagcc gcggtccaca cacactctca
     5581 aatcctggcg cggtccggca tcggtcaggg agaatatccc gaactgattg gaaaacgacg
     5641 cgcagtagac aatgatcccc agcgcagcaa ctgcccccca tgcaagcagg atgcccaaat
     5701 cttttttctc ctttttatca gcgtaaaaca tccgccagac aaaaaacacc atgagcattg
     5761 ccaggtaagt caggaactgc ggacgcagga aaatgagcca tagcgtaacc agcagcgcac
     5821 ccgcaccgtc ccaaaagcgg cgctcctgga tgtagcgaac aatcaggaaa aagaatacca
     5881 cggtggctga cagagaaagg gattctgtca aaacaacatt gtcccagcca tacaccgccg
     5941 ggcagaatcc gtacagaagg agcaccgctg cccgcacgcg ggtacccacc cccacgcggc
     6001 gcagcgtggc cgaaaacagc ggcagaacgg cgatgccaac cagctcctgc aaaaaagtgg
     6061 ctgcgtgcag ctgatgccat tcgccgacga ttatgcgaat cagatacaaa atcgtaccgt
     6121 acaccggcgc acggccattc accgcgctgc cctgcaaagc gcgtatcggg tcgtaggcca
     6181 gataatcctg cgaatcccgg tacacgatag cggtttcatg cgtataa
//
LOCUS       DBMDSQ010000095         6224 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_3206_length_6224_cov_1.483913, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000095 DBMDSQ010000000
VERSION     DBMDSQ010000095.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 6224)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6224)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6224
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3206_length_6224_cov_1.483913"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..383
                     /gene="tig"
                     /locus_tag="ACZPAX_04820"
     CDS             <1..383
                     /gene="tig"
                     /locus_tag="ACZPAX_04820"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046426.1"
                     /note="Tig; RopA; peptidyl-prolyl cis/trans isomerase;
                     promotes folding of newly synthesized proteins; binds
                     ribosomal 50S subunit; forms a homodimer; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HIR1113253.1"
                     /translation="GMTMEQFKEQFREQADKQVKMRLAMEAIVAKESIEATEEEFEAE
                     IKRIADAYQMEADKVKSLVDAAAVKKDLAVNKAIDFVKEKANIVLGAAEEEKKPAKKT
                     TRKTTKKAAAKKDEEPKEEETQGE"
     gene            501..1091
                     /locus_tag="ACZPAX_04825"
     CDS             501..1091
                     /locus_tag="ACZPAX_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HIR1113254.1"
                     /translation="MSLVPYVVEQTARGERSYDIFSRLLNDRIIVLSEDVNSASASVI
                     VAQLLYLEGQDPEKDIYFYINSPGGSISDGMAIHDTMNYIKCDVSTICVGMAASMGSF
                     LLASGTKGKRFALPNSEILIHQPLIGGQGISGQATDIKIHADHIVRIREKMNTLLSQY
                     TGQPLETITRDTERDNYMTAQQAKEYGLIDDILYKH"
     gene            1106..2461
                     /gene="clpX"
                     /locus_tag="ACZPAX_04830"
     CDS             1106..2461
                     /gene="clpX"
                     /locus_tag="ACZPAX_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HIR1113255.1"
                     /translation="MANQTSGKDGKGRELVCSFCGRSQNEVEQLVIGPGVNICKDCLD
                     MCCNALYKDGERPAPPRAGMPPRDRRRSAPQPGADYDYDPLESINILTPAEIKAGLDQ
                     YVIGQDAAKKVLAVSVYNHYKRILSGQNSDVELQKSNVLMLGPSGTGKTLLAQTLAKM
                     LNVPFAIADATTLTEAGYVGEDVENILLKLIQAADFDIPRAEIGIIYVDEIDKITRKS
                     ENPSITRDVGGEGVQQALLKIVEGTVSNVPPNGGRKHPQQEFIQINTKNILFICGGAF
                     DGLEKLIQKRTDNASMGFGSQLRTTLDKDETRQKLLKNVEPDDLVKFGLIPELIGRLP
                     VVTVLDPLDEEALVRVLTEPKNSIVRQYKELLHLDNAELVFTDAALHAIAKKTVERKS
                     GARGLRSVVEELLIPIMYDIPSDATITKVTIDADTVEGGQPHIEHGAMRPRYKSLTEQ
                     Q"
     gene            2636..2920
                     /locus_tag="ACZPAX_04835"
     CDS             2636..2920
                     /locus_tag="ACZPAX_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HIR1113256.1"
                     /translation="MKIKPLADRVVIKLVEEEETTKGGLILSGSAKEKPQVAEVIAVG
                     PGGNVDGKEVEMIVKVGDKVLTSKYSGTEVKVDGEECTIVRQSDILAVVE"
     gene            2966..4597
                     /gene="groL"
                     /locus_tag="ACZPAX_04840"
     CDS             2966..4597
                     /gene="groL"
                     /locus_tag="ACZPAX_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015538060.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HIR1113257.1"
                     /translation="MAKQIKQGEDARKALCAGIDQLANTVKVTLGPKGRNVVLSKKFG
                     SPLITNDGVTIAKEIELKDEFENMGAQLVREVATKTNDAAGDGTTTATVLAQAFVNEG
                     MKNVTAGANPMDIKRGMQKAVSAAVEAVKEHSQKVNGSKDIARVGTVSAGDAEIGQLI
                     ADAMEKVTADGVITIEENKTTAETYTEVVEGMRFDRGYVTPYMVTDTEKMETVYDDCS
                     VLITDKKISVFQDVVPLLEQVIQSGRKLLIIAEDVEGDALSNLIINRLRGGLNVVAVK
                     APGFGDRRKEMLQDIATLTGGTVISSDLGYELKDATMQLLGQARQVKVTKENTTIVGG
                     AGDKQAIADRVAQIRSQIAVATSDFDREKLQERLAKMAGGVAVIKVGAATEVEMKDKK
                     LRIEDALNATKAAVEEGIVAGGGTATINAIPAVDKLVGELEGDERTGAKIVRKALEAP
                     LRQIAANAGLEGSVVIDNILKANKPNYGFDAQKEEYVEDMIEAGIVDPTKVTRSALEN
                     AASVAAMVLTTESLVADLPEPPAPAAPAPDMGGMY"
     gene            4718..5179
                     /locus_tag="ACZPAX_04845"
     CDS             4718..5179
                     /locus_tag="ACZPAX_04845"
                     /inference="COORDINATES: protein motif:HMM:NF016763.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HIR1113258.1"
                     /translation="MKQRMKYAALAFTLGLMALIFYFSGQPAADSYKLSETVTETVQT
                     DGISFLLPLWFDAENFHANLRKWAHVAIFAALGVGMALTVHSWLSGLTLRRQGMQAAV
                     LCVLYAASDELHQYFVPGRAALVGDVVIDALGILPGVAGVCLLVWLHQKQR"
     gene            5208..>6224
                     /locus_tag="ACZPAX_04850"
     CDS             5208..>6224
                     /locus_tag="ACZPAX_04850"
                     /inference="COORDINATES: protein motif:HMM:NF013100.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HIR1113259.1"
                     /translation="MSLRRFYGLCGALVFLAGVVLCRTYWVGQQAAYAASAGGQTVQT
                     LDLPRARGDFYDRNGRKLTGTQTQYYALCIPGDDGYTALFPYVSYEKQGVLYDRRNLA
                     APFLIEVSRDLTGRGIATCAVPAHTVDIAPHLLGYLDGEGRGVAGLERAYDALLTASG
                     DKRTVSCVLAAHSGLMAGTSPVWATREEGTGQGVQLTLDADLQRACEALAAQLMPRGC
                     ILIMDAATGEVLASVSAPTFDAQDVAASIRADDTSLINRPFAAFSAGSVFKVVLAAAA
                     YEQGLDWFTHDCTGEIVVAGQTYRCAQGRAHGEVNLRGALEESCNTYFIKLGQLLGAE
                     AIRDE"
BASE COUNT         1385 a         1819 c         1880 g         1140 t
ORIGIN      
        1 tgggcatgac catggagcag ttcaaggagc agttccgtga gcaggccgac aagcaggtca
       61 agatgcgcct ggccatggag gctatcgtcg ccaaggagtc catcgaggct accgaggagg
      121 agttcgaggc tgagatcaag cgcattgccg atgcttacca gatggaggcc gacaaggtca
      181 agagcctggt cgatgccgct gccgtcaaga aggatctggc tgtcaacaag gccatcgact
      241 tcgtgaagga gaaggctaac atcgtcctgg gcgctgccga ggaagagaag aagcccgcca
      301 agaagaccac ccgcaagacc accaagaagg ctgccgccaa gaaggacgag gagccgaagg
      361 aagaggagac ccagggcgag taatcacccc gcggcatccg taaacattga gtaaaagggc
      421 cggtacggtg cgcagctttg ccgccgtatc ggttccttta tcatcaccac atttttatga
      481 taccaaggag ggaaaaccca atgagccttg taccctatgt tgtcgagcag actgcgcgcg
      541 gtgagcgcag ctatgacatt ttctcccgct tgctcaatga ccgtatcatt gtgctgagcg
      601 aggatgtcaa ctccgcctcc gccagcgtta tcgtggcaca gcttttgtac cttgaaggcc
      661 aggatccgga gaaggatatc tacttttata tcaacagtcc cggcggctcg atctccgacg
      721 gcatggccat ccatgacacg atgaactaca tcaagtgcga tgtctccacc atctgtgtcg
      781 gcatggcggc ctccatgggc tctttcctgc tggccagcgg cacaaagggc aagcgctttg
      841 cactgcccaa ctcggaaatt ctcatccacc agccgctcat cggcggacag ggcatttccg
      901 gccaggccac ggatatcaaa attcacgctg accacatcgt gcgcatccgt gaaaagatga
      961 acacgctgct cagccagtac accggccagc cgctggagac catcacccgc gataccgagc
     1021 gcgacaacta tatgaccgcg cagcaggcga aggagtacgg cctgatcgac gatatcctct
     1081 ataaacactg attcggggga acaacatggc aaatcaaacg tccggtaagg acggaaaagg
     1141 acgcgagctc gtctgcagct tctgcggacg ctctcaaaat gaggtggagc agctggtcat
     1201 cggccccggt gtcaacatct gcaaggactg cctggatatg tgctgcaacg ccctgtataa
     1261 ggatggcgag cgcccggccc cgccgcgcgc cggtatgccg ccccgggatc gccgccgcag
     1321 tgcgccgcag cccggtgccg actacgacta tgacccgctg gaaagcatca acatcctgac
     1381 gcctgctgag atcaaggcag gccttgacca gtatgtcatc ggccaggatg ccgccaaaaa
     1441 ggtgctggca gtctcggtct acaaccacta taagcgcatt ctctccggtc agaacagcga
     1501 cgtcgagctg caaaagtcca acgtcctgat gctcggcccc tccggcaccg gcaaaacgct
     1561 gttggcccag accctggcaa agatgctgaa cgtgcctttt gccatcgccg acgccacgac
     1621 cctgaccgag gccggttacg ttggtgagga cgtcgaaaac atcctgctta aactgattca
     1681 ggcggcggat tttgatattc cccgtgcaga gatcggcatt atctatgtgg acgagatcga
     1741 caagatcacc cgcaagagcg agaacccctc catcacccgc gacgtgggcg gtgagggtgt
     1801 gcagcaggca ttgctgaaaa tcgtcgaggg cacggtcagc aatgtgccgc ccaacggcgg
     1861 tcgcaagcac ccgcagcagg agttcatcca gatcaacacg aagaacatcc tgtttatctg
     1921 cggcggtgcg tttgacgggc tggaaaagct catccagaag cgcactgaca atgccagcat
     1981 gggctttggc agccagcttc gcacgacgct cgataaggac gaaacgcgcc agaaactgct
     2041 gaaaaacgtt gagccggacg atctggtcaa gtttggcctt atcccagaac ttatcggtcg
     2101 tctgccggtc gtgaccgtgc tggacccgct ggacgaagaa gcccttgtcc gcgtgctgac
     2161 cgagccgaaa aacagcattg tgcgccagta caaggagctg ctgcacctgg ataacgccga
     2221 gctggtcttt accgacgcgg cactgcacgc catcgccaaa aagacggtgg agcgcaagag
     2281 cggcgcacgc ggcctgcgca gcgtcgtcga ggaactgctg attccgatta tgtacgacat
     2341 tccttccgac gcgaccatca ccaaggtgac gatcgacgcc gacacggtgg agggcggcca
     2401 gccccacatc gagcacggcg ctatgcgccc gcgctacaaa agtttgacgg aacagcaata
     2461 aaaactacct gcaccggagc cgaaacgccc cggtgcattt ttgttcacaa aaattttata
     2521 tagaaaacac ttgatttttt gcagcaaagt ggctattata tatttagcac tcaaagagac
     2581 tgagtgctaa actaaaaata taaagagaaa tcttttatag ggaggaactt ccactatgaa
     2641 aatcaaacct cttgcagatc gtgttgttat caaactggtc gaagaagaag aaaccaccaa
     2701 gggcggcctg atcctgtccg gctccgccaa ggaaaagccc caggttgccg aagtcatcgc
     2761 tgtcggcccc ggcggcaatg tggacggcaa ggaagtcgag atgatcgtca aggtcggcga
     2821 caaggtcctg accagcaagt actccggcac cgaggtcaag gtcgacggtg aggaatgcac
     2881 catcgtccgc cagagcgaca tcctcgcggt tgtggaataa tttgattttt cagtaatttc
     2941 tttcagataa aaggagagat tcattatggc taaacagatc aagcagggcg aggacgcccg
     3001 caaggccctt tgcgctggca tcgaccagct ggccaacacc gtcaaggtta ccctcggccc
     3061 caagggccgt aacgtcgtgc tgagcaagaa gttcggcagc ccgctcatca ccaacgatgg
     3121 tgtcaccatc gccaaggaaa ttgagctgaa ggacgagttc gagaacatgg gcgcacagct
     3181 cgttcgcgaa gtcgccacca agaccaacga tgctgccggt gacggcacca ctaccgctac
     3241 cgttctggct caggctttcg tcaacgaggg catgaagaac gttaccgccg gtgccaaccc
     3301 catggacatc aagcgcggta tgcagaaggc cgtctctgct gcggttgagg ccgtcaagga
     3361 gcacagccag aaggtcaacg gcagcaagga tattgctcgt gtcggcactg tttctgccgg
     3421 tgatgctgag atcggtcagc tgattgctga cgcgatggag aaggtcaccg ccgatggcgt
     3481 catcaccatc gaggagaaca aaaccaccgc cgagacctac accgaggtcg ttgagggcat
     3541 gcggtttgac cgcggctacg tgactcccta catggtcacc gacacagaga agatggagac
     3601 cgtctacgac gactgctccg tcctgattac cgacaagaaa atcagcgtct tccaggatgt
     3661 ggtccctctg cttgagcagg tcatccagtc cggccgcaag ctgctcatca ttgctgagga
     3721 tgtcgagggc gatgccttgt ccaacctcat catcaaccgt ctgcgcggcg gcctgaacgt
     3781 cgttgccgtc aaggcacctg gctttggcga tcgccgtaag gagatgctgc aggatatcgc
     3841 caccctgacc ggcggcaccg ttatcagcag cgacctgggc tatgagctga aggatgccac
     3901 catgcagctg ctgggccagg cccgccaggt caaggtcacc aaggagaaca ccaccatcgt
     3961 cggcggtgcc ggagacaagc aggctatcgc agaccgcgtt gcccagatcc gcagccagat
     4021 cgctgttgca acctctgact tcgaccgtga gaagctgcag gagcgcctgg ccaagatggc
     4081 cggcggtgtt gccgtcatca aggttggcgc cgctaccgag gtcgagatga aggacaagaa
     4141 gctgcgcatc gaggatgctc tgaacgcgac caaggccgct gttgaggaag gcatcgttgc
     4201 tggcggcggt acggccacca tcaacgccat ccccgctgtc gacaagctgg tcggtgagct
     4261 ggaaggcgac gagcgcaccg gcgctaagat cgtccgcaag gcgctggaag ctcctctgcg
     4321 ccagatcgct gccaatgccg gtctggaagg cagcgttgtt atcgacaaca tcctgaaggc
     4381 caacaagccc aactacggct tcgacgccca gaaggaagag tacgttgagg atatgatcga
     4441 ggccggtatc gttgacccga ccaaggtcac tcgttccgct ctggagaacg ccgccagcgt
     4501 cgccgcgatg gttctgacca ccgagagcct ggtcgctgac ctgcccgagc cgccggcccc
     4561 ggctgccccg gcccctgaca tgggcggtat gtactaattc agcccaaaag caagcacaag
     4621 gtcgaagtct tggcgacaag gcttcggcct ttttgtatag acccaaggcg gtaaaacgtg
     4681 gtataataac caaaaacagc ggggggggga ggcagccatg aaacaacgga tgaaatacgc
     4741 ggcgctggcc ttcacgctgg ggctgatggc actgattttc tacttttcgg gccaaccggc
     4801 ggcggactcc tacaaactca gcgagaccgt gaccgagact gtgcagacgg acggtatctc
     4861 gtttttgttg ccgctctggt tcgatgccga gaacttccac gcgaacctgc gcaagtgggc
     4921 gcatgtggcg atttttgccg cgcttggtgt aggcatggcc ttgactgtgc acagctggct
     4981 gtccgggctg acgctgcgcc ggcagggaat gcaggcggcg gtgctgtgtg tgctgtatgc
     5041 ggccagtgac gagctgcacc agtattttgt gcccggccgc gccgccctgg tgggggatgt
     5101 ggtgatagat gccttaggca tcctgcccgg cgtggcgggg gtgtgccttt tggtctggct
     5161 gcatcaaaag caacgctgat gcccatactc ccaccggggg tgagggtatg tctctgcggc
     5221 ggttttatgg gctttgcggg gcgttggttt ttctggcggg ggtcgtgctc tgccgcacct
     5281 actgggtggg gcagcaggcc gcctatgccg ccagcgcagg cggccagacc gtccagacac
     5341 tggacttgcc ccgggccagg ggcgatttct acgaccgcaa cggccgcaaa ctgactggca
     5401 cgcaaacgca gtattacgcg ctctgcatcc ccggcgacga tggctacacg gcgctgtttc
     5461 cctatgtttc ctacgaaaag cagggcgtgc tttacgaccg ccgtaacctg gccgcgccgt
     5521 tcctgattga agtcagccgc gacctgaccg ggcggggcat cgccacctgc gcggtgcctg
     5581 cgcatacggt ggatattgca ccgcatctgc ttggctacct cgacggcgaa ggccgcggcg
     5641 tcgccgggct ggaacgcgcc tacgacgcgc tgctgacggc ctccggcgat aagcgcaccg
     5701 tcagctgcgt gctggcggcg cacagcggcc tgatggcggg gacaagccct gtatgggcta
     5761 cgagggaaga aggcaccggg cagggcgtgc agctgacact ggacgccgac ttgcagcgcg
     5821 cctgtgaggc actggcggcg cagctcatgc cgcgggggtg catcctcatt atggacgctg
     5881 ctacaggcga ggtgcttgcc agcgtcagtg cgccgacgtt tgacgcacag gatgtggccg
     5941 ccagcatccg cgccgacgat acttcgctca tcaaccgccc gtttgctgcg ttcagcgctg
     6001 ggtcggtgtt taaagtggtg ctggccgcag ctgcctatga acagggcttg gactggttca
     6061 cccacgactg caccggtgaa atcgtggtgg cggggcagac ctaccgctgc gctcagggcc
     6121 gcgcccacgg cgaagtcaac ctgcgcggtg cgctggaaga aagctgcaac acctatttta
     6181 tcaaactggg ccagctgttg ggggcagagg ccatccggga cgag
//
LOCUS       DBMDSQ010000096         6210 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_3213_length_6210_cov_1.831724, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000096 DBMDSQ010000000
VERSION     DBMDSQ010000096.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 6210)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6210)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6210
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3213_length_6210_cov_1.831724"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            358..927
                     /locus_tag="ACZPAX_04855"
     CDS             358..927
                     /locus_tag="ACZPAX_04855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113260.1"
                     /translation="MQIKRLSPAACEELYHTALPRDFPAAELKPFAEIQRLLDAGIYE
                     PLLLTDDAGVRLAYAWQVVLPGQRTALLDYFAVRCDLRGGGVGTQALQLVKAYDAMRV
                     HDLLLECEHPAEAPDPAVARRRIGFYLRAGARATALESRVFGTRYAVLSLPCGGTVPD
                     AALCKELRALYKTMIPRPYYRGNVIFYGN"
     gene            960..1403
                     /locus_tag="ACZPAX_04860"
     CDS             960..1403
                     /locus_tag="ACZPAX_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVA/SpoVAEb family sporulation membrane
                     protein"
                     /protein_id="HIR1113261.1"
                     /translation="MKLDPKQYDEMVNRASPPSPVVRDCVRAFCVGGAICTLGEGLRQ
                     LFLIWYDKTLAGTLTSIALIFLAAVFTLPGWYQKLAAKAGAGTLVPITGFANSVVSPA
                     IEFKAEGWVTGVGAKIFGIAGPVIAFGTLASFVWGVIYWLLGKVG"
     gene            1407..2423
                     /locus_tag="ACZPAX_04865"
     CDS             1407..2423
                     /locus_tag="ACZPAX_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AD"
                     /protein_id="HIR1113262.1"
                     /translation="MIRKGDTLLFDAPPTVAAWAAAGGKKESEGPLASTFDFVTQDAG
                     FGEQGCENWEQAESILQRKAAELCLRKADTPRTKVDIAFAGDLQAQCTASNYTMRELG
                     VPFAGVYGACSTMAETLGLAAAFAASGMAERAMALTSSHFCAAERQFRTPLEYGAKRT
                     PTAQWTATAAGACLVRAHGGQGVPVLSVTFGRVQDFEVHDINNMGAAMMPAAASTLLR
                     YFAATNTRPQDFDAIFTGDLGEVGSALLREQMAKEGVPLDNHQDCGCLLYNTQGQNVQ
                     AGGSGAGCSAAVLCCKILPMLAAGQLRRVLFMATGALMSQTTFLQGESIPGIAHCVEL
                     GGAV"
     gene            2420..2776
                     /gene="spoVAE"
                     /locus_tag="ACZPAX_04870"
     CDS             2420..2776
                     /gene="spoVAE"
                     /locus_tag="ACZPAX_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005929703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AE"
                     /protein_id="HIR1113263.1"
                     /translation="MNYVWAFVAGGLICVVGQLFIDYTKLSPARILTGYVVAGVILSA
                     VGLYKPLVDFAGAGASVPLLGFGHLLAQGVQNAVDECGLPGAFTGGLTAASGGIAASL
                     IFGVLAALVGKSKDQN"
     gene            2864..4201
                     /locus_tag="ACZPAX_04875"
     CDS             2864..4201
                     /locus_tag="ACZPAX_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007049323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1113264.1"
                     /translation="MQKQLTTGPVMPTMLQFAVPMILGDLLQQCYNIADTLIVGRFIG
                     TGALAAVGSAFTLMTFLTSILLGLAMGSGTVFSIKYGQKDDAGLKEGILASFVLLAGV
                     TAVLVFAVYAGFDGIIWFLRIPAEIDAMMRTYLRIVFAGLAATFLYNYFASLLRALGN
                     SVVPLVFLAVSAGLNIVLDLWFVCGLGRGVAGAAEATVIAQYVSGVGIALYTWFGFPL
                     LRAACRAKLRPARIKEIASFSALTCIQQSVMNLGILAVQGLVNSFGTVVMAAFAAAVK
                     IDAFAYLPVQDFGNAFSIFLAQNYGAQEKGRLKQGMRGAFAAAAGFSVAVSAVVYGFA
                     APLMRIFIDAGEMDVIAEGVRYLHIEGAFYLGIGWLFLLYGLYRAVGRPGMSVVLTVI
                     SLGIRVALAYTLSAIPAVGVVGIWWSVPIGWFLADAVGLGYYWWKRRTLFEMR"
     gene            complement(4366..5814)
                     /locus_tag="ACZPAX_04880"
     CDS             complement(4366..5814)
                     /locus_tag="ACZPAX_04880"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005943529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HIR1113265.1"
                     /translation="MSNEYDYSGLYNNTSGGQPAQNNAQPAQPEQTAPQQPAQGSAYP
                     NVGSSGANTANTARTDYSGCGASPQSQQDNSGYTSSFSGGNGGSGGYNGYSYASAPQQ
                     PPVPEKKKGGRKVLLRVLAGVGVVALGFGGGFGGAVAASRMGLTGSQVVVQQVQRSTD
                     TDATTTGSTGGTNMSVQQIASVVSPSVVAITTEQMSSSQTWFGGYYVQSGAGSGVIIS
                     QDGYILTCAHVVSGATSVKVQLDGSDESYDATIVGQDSTSDIAVLKIDATGLTPAVIG
                     DSDALAVGEVAVAVGNPLGTLSNTVTDGVVSALNRQVTVQDNDMTLIQTDASISPGNS
                     GGGLFNGNGELIGIVNAKSSYSEAEGIGFAIPINTAMEIGQQLIESGSVARPALGVKI
                     VDVTDAQTAQQLGVSTMGVYVVEVTKGSGAEAGGVLAGDRVLAVDDTAVSDTSALKNY
                     LKDKSIGDTVALQVERDGKVLTLNVALGSSAQ"
     gene            5998..>6210
                     /locus_tag="ACZPAX_04885"
     CDS             5998..>6210
                     /locus_tag="ACZPAX_04885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase"
                     /protein_id="HIR1113266.1"
                     /translation="MKKMILRVLLALLLLVLLAAAGFGVLYAGRLRTVNSIEKITDYS
                     DYNLYRMDVRYDYSIDDVINYGITDNQ"
BASE COUNT         1045 a         1763 c         2060 g         1342 t
ORIGIN      
        1 ccagcagctc ctgctcgatg aggtccacgt gggcgatctc ccacgcctgc tgttccaggt
       61 cgctgtaatc ctcgatataa atgccgccgt tggcgaccat cgttgcgatg gcctcggccg
      121 tgtctgcgtc acgcttggcg acggtaatgc tgatgtcagt ccattccatg tgggtggctc
      181 cttgtttata aagtgtgtcg caaatggcgg cggtcagagg actgaccgcc ctacagggca
      241 gctgcatggt agggcggcca gtcctctggc cgccgttggg cagcacccgt gatgcgtaat
      301 tttacctatt ataccgtatt ttcccccaca attcaatcac agaaaggaga aatctccatg
      361 caaatcaagc gcctgtcccc tgctgcgtgc gaagaactct accacacggc gctgccgcgg
      421 gatttccctg cggcagagct caaacccttt gccgagatac agcgcctgtt agacgcgggc
      481 atctacgagc cgctgctgct gaccgacgac gcaggtgtgc ggctggccta cgcgtggcag
      541 gtcgtgctgc cgggccagcg gacggcgctg ctggactact ttgccgtgcg gtgtgacctg
      601 cggggcggcg gcgttggcac ccaggcattg cagctcgtca aggcctacga cgccatgcgg
      661 gtgcatgatc tattgttgga gtgcgagcac cccgccgagg cccctgaccc tgccgtggcg
      721 cggcggcgca tcgggtttta cctgcgggcg ggggcgcggg ccacggcact ggaaagccgg
      781 gtgtttggca cgcggtacgc cgtgctttcc ctgccctgcg gcggcacggt tcccgacgcc
      841 gccctttgca aagagctgcg cgcactgtac aaaacgatga taccgcggcc gtattatcgc
      901 gggaacgtta tattttacgg caactaagcc catactaccc ccaaaaggag gtatggactg
      961 tgaaactgga ccccaagcaa tatgacgaaa tggtaaaccg cgcgtcgccg ccttcgccgg
     1021 tggtgcggga ctgtgtgcgg gcgttctgcg tcggcggggc tatctgcacc ctgggcgagg
     1081 ggctgcgcca gctgtttttg atttggtacg acaaaacgct ggcaggcacc ctgacgagca
     1141 ttgcgctgat tttcctcgcc gccgttttca cgctgccggg ctggtatcag aaactggccg
     1201 ccaaggcggg cgcgggtacg ctggtgccta tcaccgggtt tgccaactcg gtcgtcagcc
     1261 cggcgattga gttcaaggcc gagggctggg tgacaggcgt aggtgcaaag atttttggca
     1321 tcgccgggcc ggtcatcgcc tttggcacgc tggccagctt tgtgtggggc gtcatctact
     1381 ggctgctggg aaaggtgggg tgacacatga tacgaaaggg tgataccctg ctgtttgacg
     1441 cgccgcccac cgtggcggcc tgggcggcgg cgggcggcaa aaaggagagc gaaggcccgc
     1501 tggcctcgac ctttgacttc gtgacccagg acgcgggctt tggtgagcag ggctgcgaaa
     1561 actgggagca ggccgagagc attttgcagc gcaaggcggc ggaactctgc ctgcgcaagg
     1621 cggatacacc gcgcacgaag gtggatatcg cctttgccgg cgatttgcag gcccagtgta
     1681 cggccagcaa ctacacgatg cgggagctgg gcgtgccgtt cgcgggcgtg tacggcgcgt
     1741 gttctacgat ggcagagacg ttggggctgg ccgctgcgtt tgcggcgtcc ggtatggcag
     1801 aacgcgccat ggcgctgacc agcagccatt tttgcgcagc agaacggcaa ttccgcacac
     1861 cactggaata cggcgccaag cgcaccccca cggcccagtg gacggccaca gcggcgggag
     1921 cctgcctggt gcgggcgcac ggcgggcagg gcgtgccggt gctctcggtg acgtttggcc
     1981 gcgtgcagga ttttgaggta cacgacatca acaacatggg cgcggccatg atgcccgccg
     2041 cggcatcaac gctgctgcgc tattttgcgg cgaccaacac ccgcccgcag gactttgacg
     2101 cgatttttac cggggacttg ggcgaggtcg gctcggccct tttgcgagag cagatggcaa
     2161 aggagggcgt accgctggac aaccaccagg actgcggctg cctgctgtat aacacacagg
     2221 gccagaatgt gcaggcgggc ggcagcgggg ccgggtgcag cgcggcggtg ctgtgctgta
     2281 aaatactgcc catgctggcg gctggtcagc tgcgccgcgt gctgtttatg gccaccgggg
     2341 cgctgatgag ccagaccacg tttttgcagg gcgagagcat tccaggcatt gcccactgcg
     2401 ttgagctggg aggtgcggta tgaactacgt ctgggcgttt gtggcaggcg gcctgatttg
     2461 cgtcgtgggg cagttgttta tcgattatac caagctctcc cctgcccgga ttttaaccgg
     2521 gtatgtggtg gcgggcgtaa tcttaagcgc cgtgggtctg tacaagccgc tggtggactt
     2581 tgccggggct ggggccagcg tgccgctgct ggggtttggg cacctgctgg cccagggcgt
     2641 acagaacgcc gtggacgagt gtgggctgcc cggcgcgttt accggcgggc tgacagccgc
     2701 cagcgggggg attgcggcta gtttgatttt tggagtattg gccgcgttgg tggggaaaag
     2761 taaggatcag aactgagttg agaaagcctt cctttgacaa ggaaggcctt tctgttttat
     2821 actaggtatg gtgtaaaaac agaagaagag gttttcagct ctcatgcaaa aacaactcac
     2881 tacaggcccg gtcatgccta cgatgctgca gtttgcggtg cctatgattt tgggcgattt
     2941 gctgcagcaa tgctataata ttgccgatac cctcattgtg gggcggttca tcggcaccgg
     3001 ggcactggcg gcggtggggt cggcgttcac gcttatgacc tttttgacgt ccattctgct
     3061 gggtcttgcc atgggcagcg gcacggtgtt ttccatcaaa tacggacaaa aggacgatgc
     3121 agggttaaaa gagggcatcc ttgccagctt tgtgctgctg gcgggtgtca cggcggtgct
     3181 ggtgtttgct gtgtacgccg ggttcgacgg cattatctgg ttcctgcgca tccctgccga
     3241 gatcgacgcc atgatgcgga cgtacctgcg catcgtgttt gcgggactgg ctgcaacatt
     3301 tctctataat tattttgcgt cgctgctgcg ggcgctgggc aactcggtcg tgccgctggt
     3361 gttcctggcg gtgtcggcgg ggttgaacat cgtgctcgac ctttggttcg tctgcgggct
     3421 ggggcgtggc gtggccggtg ctgccgaggc gaccgtcatt gcgcagtacg tctccggcgt
     3481 gggcattgcg ctgtacacct ggttcgggtt cccgctgctg cgggcggcct gccgggccaa
     3541 gctgcggccc gcccgcataa aagaaatcgc gtccttctcg gcgctgacct gcatccagca
     3601 gtctgtcatg aatctgggca tcctggccgt gcagggtctt gtcaacagct ttggcacggt
     3661 ggtcatggcg gcgtttgcgg cggcggtcaa aatcgacgcc tttgcgtacc tgccggtgca
     3721 ggatttcggc aatgcgttct cgattttctt ggcgcagaac tacggtgcac aggagaaagg
     3781 ccgcctcaaa cagggtatgc gcggcgcgtt tgccgcggcg gcagggttca gcgtggcggt
     3841 cagcgctgtg gtgtatgggt ttgccgcgcc gctgatgcgg atctttattg atgcgggcga
     3901 gatggatgtc atcgccgagg gtgtgcggta tctgcacatc gagggcgcgt tttatttagg
     3961 catcggctgg ctgtttttgc tgtacggcct ataccgcgcc gtggggcggc cgggtatgtc
     4021 ggtggtgctc acggtcattt cgctgggcat ccgcgtggcg ctggcctata cgctttcggc
     4081 aatcccggcg gttggcgtgg tcggcatctg gtggagcgtg cccatcggct ggtttttggc
     4141 cgatgctgtg gggctggggt attattggtg gaaacggcgg acgttgtttg agatgcggtg
     4201 aaaaccgcta ggcagggttg caggggccgc agatgtgcgg cccgcagcct tcccgcaaac
     4261 gaccgttctc cctatacgac cgggccgggc atgcccggcc cctacggata aatatcaaaa
     4321 aagggatgca ggccgcaaaa cctgcatccc ttttcttatg ctattttact gtgcgctcga
     4381 ccccagggcg acgttcagcg tcagcacctt accgtcgcgc tcgacctgca aggccaccgt
     4441 gtcgccgatg gatttatcct tcagatagtt cttcaacgcg gaagtgtcgc tcaccgccgt
     4501 gtcatccacg gccagcacgc ggtcgcccgc cagcacgccg ccggcttcgg caccgctgcc
     4561 tttggtgact tccaccacgt aaacgcccat ggtggaaacg cccagctgct gcgcggtctg
     4621 tgcgtcggtc acatcaacga tcttgacgcc cagcgccgga cgcgccacgc tgccgctttc
     4681 aatcagctgc tggccgattt ccatggctgt gttgatgggg atggcaaagc cgatgccctc
     4741 ggcttcactg tagctgctct tggcgttgac aatgccgatc agctcgccgt tgccgttgaa
     4801 caggccgccg ccagagttgc ccgggctgat ggacgcatcg gtctggatca gcgtcatatc
     4861 gttgtcttgc accgtgacct ggcggttcag ggcgctgaca acgccgtcgg tgacggtgtt
     4921 ggacagcgta cccagcgggt tgccgacggc cacagcgact tcacccacgg ccagcgcgtc
     4981 actgtcgccg atgaccgccg gggtcaggcc ggtggcgtcg attttcagta ccgcgatatc
     5041 ggaggtggaa tcctggccga cgatggtcgc gtcataactc tcatcgctgc cgtccagctg
     5101 gaccttaaca ctggttgcgc cgctgacaac gtgggcgcag gtcagaatgt agccatcctg
     5161 gctgatgatg acgccggaac cggcaccgct ctgcacgtag tagccgccga accaggtctg
     5221 gctgctgctc atctgctcgg tggtgatggc cacgacgctg ggcgagacga cagacgcgat
     5281 ctgctggacg ctcatgttcg tgccgccggt gctgcctgtg gtggtggcgt cggtgtccgt
     5341 actgcgctgc acctgctgca caacgacctg gctgccggtc aggcccatgc ggctggcggc
     5401 cactgcgccg ccaaaaccgc cgccgaagcc cagcgccaca acgccgacac cggccagcac
     5461 gcggagcagg accttgcggc cgcccttctt cttctcgggc acagggggct gctgcggggc
     5521 gctggcgtag ctgtacccat tgtagccgcc gctgccgcca ttgccgccgc tgaagctgct
     5581 ggtgtagccg ctgttgtcct gctgggactg cgggcttgcg ccgcagccgg aataatccgt
     5641 gcgggcggtg tttgcggtgt tggccccgct ggaacctaca ttggggtagg cgctgccctg
     5701 tgcgggctgc tgcggggcgg tttgttcggg ctgtgcaggc tgcgcattgt tctgtgcagg
     5761 ctgcccgccg gatgtattgt tatagagacc ggagtaatcg tattcgttgc tcatatgggt
     5821 cgtctccttt cgctttcgat gcttacagta tacagggtaa ttgtgaaata ggtttcgtca
     5881 gagagtgaaa actctgtgaa aagcggtgaa aaccgtgata aattctccgt tgcgccgcgc
     5941 aacaggatgt gctataatca aattataaag tatacatata aaagggaggc gttttctgtg
     6001 aaaaagatga tcctgcgtgt tctgttggca cttttgctgc tggtattgct ggcggcagcc
     6061 gggtttggcg tgctgtatgc cgggcggctg cgcacggtga acagcattga aaaaatcacc
     6121 gattacagcg actataatct ctaccgcatg gacgtccggt acgattacag catcgacgat
     6181 gtcatcaact acggcatcac cgacaaccaa
//
LOCUS       DBMDSQ010000097         6184 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_3225_length_6184_cov_2.029262, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000097 DBMDSQ010000000
VERSION     DBMDSQ010000097.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 6184)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6184)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6184
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3225_length_6184_cov_2.029262"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..465)
                     /locus_tag="ACZPAX_04890"
     CDS             complement(<1..465)
                     /locus_tag="ACZPAX_04890"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015563723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016994 - precorrin-6A reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6A/cobalt-precorrin-6A reductase"
                     /protein_id="HIR1113267.1"
                     /translation="MNVVIFSGTTEGRALSCQVAALGAAVTVCVATDIGAEEQGNTEG
                     ITVCAGRLTADEMAALLRGAALCVDATHPYATEATRNIRAAAQAVGVEYHRLLRPASA
                     LPAGSVTLQTAAQAAAYLADKPGNVLLTTGAKELPAFAVLDPARLYPRVLPTL"
     gene            complement(462..1202)
                     /gene="cobJ"
                     /locus_tag="ACZPAX_04895"
     CDS             complement(462..1202)
                     /gene="cobJ"
                     /locus_tag="ACZPAX_04895"
                     /EC_number="2.1.1.131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030789 - precorrin-3B
                     C17-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-3B C(17)-methyltransferase"
                     /protein_id="HIR1113268.1"
                     /translation="MNRNLVYVVGLGPGSAQFLTAQARAALQNADVLCGYTVYIDLVR
                     PLFPEKEVYATGMTREIDRCRWALEKAQRSKTVALVCSGDAGVYGMASPLLELAGNYP
                     DVEVEIVPGLTAALSGGAVLGAPLAHDFCVISLSDRLTPWAVIEKRLTCAAAGDFCVA
                     LYNPSSRGRADYLAKAVRVLLANGKHPETVCGIVRNIGRDGQTARLLTLAELESTPVD
                     MFTTVYIGNDATQNLRGRMVTPRGYHGV"
     gene            complement(1192..2196)
                     /locus_tag="ACZPAX_04900"
     CDS             complement(1192..2196)
                     /locus_tag="ACZPAX_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016321222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin 5A hydrolase"
                     /protein_id="HIR1113269.1"
                     /translation="MICAYIAFTARGLALAQRLAAACPGSVARGGADGVPLADWTARQ
                     FAQSDALIFVGAAGIAVRAIAPHCRSKAADPAVVVLDEGGQFAIPLLSGHLGGANDLA
                     RLLAAVCGAVPVITTATDGRGVFAVDEWARRQNCAVAEPERIKKISGALLAGEPVRYA
                     ADWPIQGTPPAGVMPTDTADCADFALTLTPRGEALHVVPRIAALGIGCKRGTSAEKLE
                     AVFAAFCAEHRLALQGIMQAASIDLKQDEPGLAAFCHAHGWPMDFYTADELRTAPGNF
                     TASNFVQSVTGVDNVCERAAVLASGGELLLPKWAHDGVTFAVACAPFAPDWRWKNDEP
                     "
     gene            complement(2193..2951)
                     /gene="cobM"
                     /locus_tag="ACZPAX_04905"
     CDS             complement(2193..2951)
                     /gene="cobM"
                     /locus_tag="ACZPAX_04905"
                     /EC_number="2.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005943270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046026 - precorrin-4
                     C11-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-4 C(11)-methyltransferase"
                     /protein_id="HIR1113270.1"
                     /translation="MVHFVGAGPGAPDLITRRGAALLQTADCIIYAGSLVNPALLGLA
                     KADCAIYNSAEMTLEQVLSVMKENETAHKTTVRLHTGDPCLYGAIREQMDALDAAGIP
                     YDDTPGVSSFCGAAAALNAEYTLPGVSQTVIITRMEGRTPVPEGERLAALAAHGATMV
                     IFLSIGLVEKVQAALLQGAYTVQTPAAVVYKATWPEQKIVHCTVGNLAQHTREAGITK
                     TALIVVGDFLAARYDRSKLYDPAFTTAFRRGTDA"
     gene            complement(2953..4854)
                     /gene="cbiD"
                     /locus_tag="ACZPAX_04910"
     CDS             complement(2953..4854)
                     /gene="cbiD"
                     /locus_tag="ACZPAX_04910"
                     /EC_number="2.1.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015563719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-5B (C(1))-methyltransferase
                     CbiD"
                     /protein_id="HIR1113271.1"
                     /translation="MSYCETQALAIGYGAPLLRDIALHAERGKILAFIGPNGAGKSTL
                     LKTLAAQLPTQSGAVLMDGQNLAAYTPNARARKLALMLPHTVRTELTTCFDVAAAGRY
                     PYTGRLGVLSAADKQQVRAALELVQAAPLADRDFTKISDGQRQRVLLARAVCQQPEIL
                     LLDEPTSFLDAKGKAELMAILQTLAHEKNVAIIVTLHELELAQKLADAVVCVAPSGVS
                     GVLTPQEAFAEQNIRRLFDLTAEQYAMLFKNGDTKPKFEHYIRSGQKLLRCGYTTGTC
                     AALGAAGAARLLLTGHVPESVGLRTPKGIVVEVAPQFCRPTADGAECAIVKDGGDDID
                     ATTGLPIVAAVTLLPDAPRTVTIDGGAGVGRVTKPGLDQPVGAAAINHVPRQMITEAL
                     LKEADAVGYGGGFAVTVSIKGGAAAAKRTFNPHIGVEGGLSVLGTSGIVEPMSQQALL
                     DTLQIEIHQAALKSRRLILAPGNYGLDYLAAHMPELARIPVVKISNFVGEALDMAATE
                     HFAEVLLVGHVGKLVKLAGGIMNTHSKQADCRTELFCAHAALCGADAATCRALMDAVT
                     TDACLEILDAANLREPVVASLLDAIQTHVDRRAAEAFRVGAVLFSNQAGPLGQTKTAD
                     TLLKTWKGA"
     gene            complement(4851..5870)
                     /locus_tag="ACZPAX_04915"
     CDS             complement(4851..5870)
                     /locus_tag="ACZPAX_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015563718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HIR1113272.1"
                     /translation="MHTRTRLTIAYFAMAAALLALFALNLFWGSVALSPAQVVAALLG
                     RAPDALTANILCQLRLPRAVMVVLLGAALSAAGYLLQTFFANPIAGPFVMGISSGAKL
                     AVALTMVVFLNRGLLTNSWTLILAAFAGAMAAMLFVLAVARRVQRMSILVICGVMIGY
                     ICSAVTDIVVAFAQDSNIVNLHNWSMGSFSGMTWGNVTTAAAIVLPCLAAAFLLSKPM
                     AAYQMGESYAASVGVPVRVFSVVLVLLSSLLAACVTAFAGPISFVGIAVPHLVKQTLG
                     SAKPLYVLPGCALGGAAFCLLCDLIARSLFAPTELSISSVTAVFGAPVVIWLLVRRHS
                     GEGAA"
     gene            complement(5881..>6184)
                     /locus_tag="ACZPAX_04920"
     CDS             complement(5881..>6184)
                     /locus_tag="ACZPAX_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005935011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR1113273.1"
                     /translation="SNNLATVNISLEDFYAGAKDADVLIYNSTIEGELRSIDELIAKC
                     PMLADFKAVQSGNVWCTAQSLFQQSMELPDLILDMNKVFTEGTPNADELKFLTKLQ"
BASE COUNT         1133 a         2057 c         1910 g         1084 t
ORIGIN      
        1 cagcgtcggc agcacccggg ggtacagccg cgccgggtcc agcaccgcga aggcgggcag
       61 ttccttggcc ccggtcgtca gcaggacatt tccgggtttg tccgccaggt aggcggcggc
      121 ctgggcggcg gtttgcagtg tcacactgcc cgccggcagg gcgctggccg ggcgcaacag
      181 gcggtgatat tccaccccca cagcctgggc cgctgcacgg atattgcgcg ttgcctccgt
      241 ggcatagggg tgggtcgcgt caacgcagag cgccgcaccg cgcagcaatg ccgccatctc
      301 gtcagcggtc agccgaccgg cgcagactgt gatgccctcg gtgtttccct gctcctcggc
      361 accgatatcg gtcgccacgc agaccgtgac cgccgcgccc agcgccgcca cctggcagga
      421 gagagcgcgg ccctcggtcg tgccgctgaa aattaccaca ttcatacgcc gtgatacccc
      481 ctgggcgtca ccattctgcc gcgcaaattc tgcgtagcat cgtttcctat atacaccgtc
      541 gtgaacatat ccaccggcgt gctttcgagt tcggccaatg tcagcagcct tgcggtctgg
      601 ccgtcacggc ctatgttgcg tacaatgccg cagaccgtct cggggtgctt gccgtttgcc
      661 agcaggacgc gcacggcctt ggcaaggtag tcggcgcggc ccctggacga cggattgtag
      721 agcgccacgc agaagtcccc cgccgccgcg caggtcagcc gcttttcaat cactgcccag
      781 ggcgtcagcc ggtcggacag cgagatgacg caaaaatcat gcgccagcgg tgcgcccagc
      841 acggccccgc cgctcaatgc cgcggtcagg ccggggacga tttcgacttc tacgtccggg
      901 tagttccctg ccagctccag cagcgggctt gccatgccgt agacccccgc gtcgccagag
      961 cagaccagcg ccactgtttt actgcgctgc gctttttcca acgcccagcg gcagcggtcg
     1021 atttcccgcg tcatgccggt ggcgtagacc tctttttcag ggaacagcgg ccgcaccaga
     1081 tcgatgtaga ccgtgtagcc gcagagcaca tcggcatttt gcagcgcggc ccgggcctgg
     1141 gcggtcaaaa actgcgcgct gccggggccc aggcccacca catacacaag gttacggttc
     1201 atcatttttc catctccagt ccggggcaaa gggcgcacat gccaccgcga aggtcacgcc
     1261 gtcgtgcgcc cacttcggga gcagcaactc accgcccgac gccagcaccg ccgcgcgttc
     1321 acagacattg tccacgccgg tgacgctctg cacaaaattg gacgccgtaa aattccccgg
     1381 tgccgtgcgc agttcatcgg cggtataaaa gtccataggc cagccgtggg catggcagaa
     1441 cgccgccagg cccggctcat cctgtttcaa atcgatgctg gcggcctgca taatgccctg
     1501 cagggccaga cgatgttccg cacagaacgc cgcaaacact gcttccagct tttccgctga
     1561 tgtgccgcgc ttgcagccga tccccagcgc cgcaatgcgt gggacgacgt gcaaggcttc
     1621 gccccggggc gtcagcgtca gcgcaaaatc tgcgcagtcg gcggtgtcgg tgggcatcac
     1681 acctgcgggc ggggtaccct gaatgggcca atcggcggca tagcgcaccg gctcacccgc
     1741 caacagtgcg ccgctgattt tcttgatgcg ctccggctcg gccacggcgc agttctggcg
     1801 cctggcccat tcatccacag caaaaacgcc gcgcccatcg gtggccgtcg tgatgaccgg
     1861 caccgcgccg caaaccgccg ccagtagccg cgccaggtcg tttgcgccgc ccagatgccc
     1921 agacaacagc gggatagcaa actgaccgcc ctcgtccagt accacaacgg cagggtcagc
     1981 ggctttgctg cggcaatggg gcgcgatggc ccgcaccgca attccggcag cccccacaaa
     2041 aatcagtgca tcactttgtg caaactgccg agccgtccag tcggccagcg gcacgccgtc
     2101 tgcgccgccg cgggccacac tgccagggca cgcggccgcc agccgctgcg ccaatgccag
     2161 cccccgcgcc gtgaaggcga tatacgcgca aatcatgcgt cggtccccct gcggaacgca
     2221 gtggtgaacg ccgggtcgta gagcttgctg cgatcgtacc gcgcggcgag gaagtccccc
     2281 acgacgatga gcgcggtttt ggtgatgccg gcctcgcggg tgtgctgcgc caaatttcca
     2341 acggtgcaat gtacgatttt ttgttccggc caggtggcct tgtagacgac ggcggcaggg
     2401 gtctgtaccg tatacgcccc ctgcaaaagc gcggcctgga ctttttccac aaggccgatg
     2461 gagaggaaaa tcaccatcgt ggccccatgg gcggccaatg cggccagccg ctccccttcc
     2521 gggaccgggg tgcggccctc catccgggtg atgatgacgg tctggctgac accgggcagt
     2581 gtgtactcgg cattcagtgc cgccgccgcg ccgcaaaagc tggacacgcc gggggtgtcg
     2641 tcatagggaa tgcctgcggc atccaatgcg tccatctgct cacgaattgc accatacaag
     2701 caggggtcgc ccgtgtggag ccgtacggta gttttgtgcg ctgtttcgtt ctctttcatc
     2761 acggaaagca cctgttccag tgtcatttcc gcactgttgt aaatggcgca gtccgccttt
     2821 gcaagcccca gcagcgccgg gttgactagg ctgcccgcgt agatgatgca gtccgccgtt
     2881 tgcagcagcg ccgcaccgcg ccgggtgatg agatccggtg cgccggggcc agcccccaca
     2941 aaatgtacca tgtcaggctc ctttccatgt tttcaacagt gtatcggcgg tttttgtctg
     3001 gcccagcggc ccagcctggt tggagaacag caccgccccc acccgaaacg cctccgccgc
     3061 gcggcggtct acatgcgttt ggatggcatc cagtaggctt gccacgaccg gctcgcgcag
     3121 gttggcagcg tccagaattt ccaggcaggc gtcggtagtc actgcgtcca tcagggcgcg
     3181 gcaggtggcc gcatccgccc cgcagagcgc cgcatgggcg cagaacagct cggtgcggca
     3241 atcagcctgc ttggagtggg tgttcatgat gccgcccgcc agcttgacca gcttgcccac
     3301 atggccgacg agcagcactt ccgcaaagtg ttcggttgct gccatatcca gcgcttcacc
     3361 gacaaaattt gagattttga cgacgggaat tctggccagc tccggcatat gcgcagccag
     3421 ataatccagc ccatagttgc cgggggccag gattaggcgg cggcttttca gcgcggcctg
     3481 atggatttcg atttgcagcg tgtccaaaag tgcctgttgg ctcatcggct cgacgatacc
     3541 gctggtaccc agcacggaca gcccgccctc gaccccgata tgcgggttga aggtgcgctt
     3601 ggccgcagcc gcgccgccct tgatggagac agtgaccgcg aagccgccgc catagcccac
     3661 ggcgtcggcc tctttcaaca gagcctcggt gatcatctgg cgcggcacat ggttgatggc
     3721 tgccgcaccc accggctggt ccagcccggg ctttgtcacg cggcccacac ctgcgccgcc
     3781 gtcgatggtg acggtgcgcg gcgcatccgg cagcagcgtg accgcggcaa cgatcggcag
     3841 gccggtggtg gcatcgatat cgtcgccgcc gtccttgacg atggcgcatt ctgcgccgtc
     3901 cgccgtgggg cggcagaact gcggcgcgac ctcgaccacg atgcccttgg gggtacgcag
     3961 gccgacagat tccggcacat ggccggtcag cagcagccgc gccgcccccg ccgcccccag
     4021 cgccgcacag gtgccggtgg tgtagccgca gcgcagcagc ttttgcccgc tgcggatata
     4081 gtgctcaaat ttcggctttg tatcgccatt tttaaacagc atggcgtact gttcggcagt
     4141 caaatcaaac agtctgcgga tgttctgctc ggcaaaggcc tcctgcggcg tcagcacgcc
     4201 ggataccccg gacggcgcaa cgcagacgac ggcatcggcc agcttttgcg ccagttccaa
     4261 ctcgtgcagc gtcacgatga tagcgacgtt tttttcatgg gccagcgttt gcagtatggc
     4321 catcagctcc gctttgcctt ttgcgtccaa aaacgacgta ggctcatcca gcagcaaaat
     4381 ctccggttgc tggcagacgg cacgggccag cagcacgcgc tggcgctggc cgtcgctgat
     4441 cttggtaaaa tcccggtcag ccagcggtgc ggcctgcacc agttccagcg ctgcccgcac
     4501 ctgctgtttg tcggcggcag agagcacccc cagccgcccg gtgtaagggt agcgcccggc
     4561 agcagccacg tcaaagcagg tcgtcagctc ggtgcggacc gtgtggggca gcatcagcgc
     4621 cagcttgcgg gcgcgggcgt tgggggtgta ggctgccagg ttttgcccgt ccatcagtac
     4681 cgcaccgctc tgtgtgggca gctgcgccgc caacgtcttt aataaggtgg acttgcccgc
     4741 gccgttgggg ccgatgaagg ccagtatctt gccgcgctca gcgtgcaggg caatgtcccg
     4801 cagcagcggt gcgccatagc cgatggcgag ggcctgtgtt tcgcaatagc tcatgctgcc
     4861 ccctccccgc tgtggcggcg caccagcagc cagatgacca ccggcgcacc gaacaccgcc
     4921 gtgaccgagc tgatcgaaag ttctgtcggc gcaaacaggc tgcgggcaat caggtcacac
     4981 agcaggcaga acgccgcgcc gcccagagcg cagccgggca gcacatacag cggttttgca
     5041 ctgcccagcg tttgttttac caaatgcggc accgcgatac ccacaaagga tatcggcccg
     5101 gcaaacgccg tgacgcaggc cgccagcaaa ctggacagca gcaccagcac caccgagaac
     5161 acccgcaccg gcacaccgac gctggcggcg tagctttcgc ccatctggta ggcagccatc
     5221 ggcttggaga gcaggaacgc cgctgccagg cagggcagca caatggccgc tgccgtggtg
     5281 acgttgcccc aggtcatgcc agagaagctg cccatcgacc agttgtgaag gttgacaatg
     5341 ttggaatcct gcgcgaaggc cacgacgata tccgtgaccg ccgagcagat gtagccgatc
     5401 atgacgccgc agatgaccag aatactcatc cgctgcacgc ggcgcgccac agccagcaca
     5461 aacagcatgg ccgccatggc cccggcgaaa gccgccagaa tcagcgtcca tgagttggtg
     5521 agcagcccgc ggttgaggaa cacgaccatc gtcagcgcga cggccagctt ggccccgctg
     5581 gaaatgccca taacgaacgg ccccgcaatg gggttggcaa agaatgtttg cagcaggtag
     5641 cccgccgccg acaaagccgc gcccagcagc acgaccataa ccgcccgggg cagccgcagc
     5701 tggcacagga tgttggcggt cagcgcatcg ggggcgcggc ccagcagcgc ggccacgacc
     5761 tgcgccgggg aaagcgccac gctgccccag aagagattga gtgcaaacag cgccagcagg
     5821 gccgccgcca tggcgaaata tgcgattgtc aggcgtgttc gggtgtgcat ggtatcgtcc
     5881 ttattgcagt ttcgtcaaaa atttcaattc atcggcattg ggggtgcctt cggtgaacac
     5941 cttattcatg tccaggatca aatccggcag ttccatactc tgctggaaaa ggctctgtgc
     6001 ggtacaccag acattgccgc tttgcacagc cttgaagtcg gccagcattg ggcatttggc
     6061 aatcaattca tcgatagagc gcagttcacc ctcaatcgtg ctgttataaa tcagcacgtc
     6121 ggcatccttg gccccggcgt agaaatcttc cagcgaaata ttcacggtcg ccaggttgtt
     6181 gctg
//
LOCUS       DBMDSQ010000098         6164 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_3241_length_6164_cov_1.534059, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000098 DBMDSQ010000000
VERSION     DBMDSQ010000098.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 6164)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6164)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6164
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3241_length_6164_cov_1.534059"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(6..1121)
                     /locus_tag="ACZPAX_04925"
     CDS             complement(6..1121)
                     /locus_tag="ACZPAX_04925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113274.1"
                     /translation="MQKCSLCGGRVVNGRCEECGLPIPPEHTYTLRGESAHYHRVNGE
                     DVLHRVRPTGSRPKVDGYDEEERTAHVHESGTDRHNAPRSAVRTARVEHHIEAPSRRR
                     RSANVAWLIIVVALFLILPMLQVLLQREIFSSVSEPVPEFSEEQAIEADSNAYDDLRG
                     TVPAAGADWDGNLTAGLYTVGQDLPEGTYTIQCAESNTSIDLHIVSDEHGVEIYEDLS
                     SNGYDTSDSERDDVSLPTGTVLFLSGNGTLYFHSSNAQVDALPTETAPNPLVSDAAYT
                     LSDSEDSLYLLVGEDIEPGVYDVTCTAGTGYFSFVLPMENTRTMYCSAWLYGDPDYVD
                     TLRHITLTEDTEVTLSNYSDMDFTVTLTPSETVYVHE"
     gene            complement(1310..2482)
                     /locus_tag="ACZPAX_04930"
     CDS             complement(1310..2482)
                     /locus_tag="ACZPAX_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007045722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR1113275.1"
                     /translation="MVSLLLPIIYLAFISLGLPDALLGAAWPSMYPQLGAGVAWAGGV
                     SMIISAGTIVSSLASERLNTRLGTGRVTALSVATTAVALFGFSTCTQFWQLCLWAIPY
                     GLGAGSVDAALNNYVALHYESRHMSWLHCMWGIGAAGGPVIMGWALAGSTWQNGYRII
                     SILQIVLTAVLIFSLPLWQTPADAGAGEDFTPEHRTLPQLMKVPGVPEVMCCFALYNG
                     LEAVAGMWAASYCTLVRGLDATTAASWASLFYLGITGGRFLSGFLTMRFTDHQMIRLG
                     QTLIAVGIALLLLPGSAALPVGLVVIGLGCAPIYPCIIHETPANFGKNLSMAMTGIQM
                     AAAYVGVTLLSPFFGVLAQNITMQLYPWALLAMLLLMVVLSEQLHKKTAASRAGKV"
     gene            2607..3512
                     /locus_tag="ACZPAX_04935"
     CDS             2607..3512
                     /locus_tag="ACZPAX_04935"
                     /inference="COORDINATES: protein motif:HMM:NF014376.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HIR1113276.1"
                     /translation="MALSVCRTVIDDFARELLKHGTAAFPIACYHDDLQTEPVPWHWH
                     EELEVLVVSEGTILATAAGEKYRLEKGEGLFINAGVLHGDWPLNAGPCRLHSMVFHPR
                     LVGGSPDSVFWQKYLQPLLTDPSRPCAVLRPGVEWQREAMNQMERAFRAVVNEQPGYE
                     FKVRAALLEMLFQLVGHAPAVQALPKKALRSTDRIKTMLQFIQEHYAEDVSVEQIAAS
                     AAISPSECLRCFHDMIGTTPNQYLRDQRTQRAAELLCGTGLQVSEIAAQCGFQDASYF
                     ARAFRQVYGCGPTEYRRKARRAGAV"
     gene            complement(3630..5420)
                     /gene="thrS"
                     /locus_tag="ACZPAX_04940"
     CDS             complement(3630..5420)
                     /gene="thrS"
                     /locus_tag="ACZPAX_04940"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HIR1113277.1"
                     /translation="MKIIYKDGTVAECPQEEELHVLRHTAAHIMAQAIKRLYPQADFA
                     FGPATENGFFYDVDLGDTKLTDEDLAAIEKEMKKICKENLPIKPFILPRDEAVKLMEE
                     RQEHYKIEHMADLADETEFSFYQQGEYVDMCIGPHLTYTKALKAFKITQQSGAYWKND
                     KENKMLTRINGVAFRNQEELDAWEKQQQEARERDHRKIGKEMRLFMTDDLVGRGLPMF
                     LPNGYTVWQQLEDYIKGKERERGYLHVMAPCIGTVNLYKTSGHWDHYRENMFPAMEME
                     GESYVLRPMNCPHHMMIYANQPHSYRQLPIRIGEIAHDFRYESSGTLKGIERGRHFCQ
                     NDAHLFCTPEQIKSEVANVCALIFDVYKDFNITDYRCVLSLRDPADKKKYHDDDAMWN
                     HAENALREVLTELGIHFTEEIGEAAFYGPKLDVNVKPAVGAEYTLSTCQLDFCLPAKF
                     HLTYVDKDGSEKTPVVLHRAILGSLDRFMAYLIEETKGRFPTWLAPTQVKVLPVSEKT
                     LDYAKDVAAKLQAAGVRVVLDESNEKIGYKIRQAQQVDRVPYMLVLGAKEVEANNISV
                     RDRKGETTTMELDAFIAKVVDEIKNRVNNG"
BASE COUNT         1197 a         1791 c         1895 g         1281 t
ORIGIN      
        1 tttttttatt catgcacata caccgtttca ctgggggtca gcgttacggt aaaatccata
       61 tcgctgtagt tggagagcgt gacctcggtg tcctcggtca gggtgatgtg gcggagcgta
      121 tcgacatagt cggggtcgcc gtagagccat gcgctgcagt acatcgtgcg ggtattttcc
      181 atcggcagga caaaggaaaa atatccggtg cccgccgtgc aggtcacgtc gtagacgccg
      241 ggttcgatgt cctcgcccac aagcagatac aggctgtcct cgctgtcgct gagggtgtac
      301 gccgcatcgc tgaccagcgg attgggtgcg gtctcggtgg gcagcgcatc gacctgggcg
      361 ttgctgctgt ggaagtacag cgtgccattg ccgctcagaa acagcacggt gcctgtcggc
      421 agagacacat cgtcccgctc actgtcgctg gtgtcatagc cgtttgagga cagatcctcg
      481 tagatttcaa caccgtgctc atcgctgacg atgtgcaggt caatggatgt attgctctcg
      541 gcacactgga tggtgtaggt gccctcgggc agatcctgcc ctacggtgta caggcccgcc
      601 gtcaggttgc cgtcccagtc cgcacccgcg gcgggcaccg tgccgcgcag gtcgtcatag
      661 gcgttgctgt cggcctctat ggcctgttct tcggagaatt cgggaacggg ctcggaaacc
      721 gaggaaaaga tttcacgctg cagcaggacc tgcagcatgg gcaaaatcaa aaacagcgcc
      781 acaacgatga taagccacgc aacattggca ctgcgtctgc gtctggaggg tgcctcgatg
      841 tggtgctcca cccgggccgt gcgcaccgcg gagcgcggtg cattgtggcg gtctgtgccg
      901 ctctcatgta catgggcggt gcgctcttcc tcgtcgtagc cgtccacctt ggggcggctg
      961 ccggtgggcc gcacgcggtg cagcacatcc tcgccgttga cgcggtggta gtgggcgctt
     1021 tcgccccgca gggtgtaggt atgttcgggc gggatgggca gaccgcattc ctcacatcgg
     1081 ccattgacga cccgcccgcc gcacaggctg catttttgca tggtatcgcc tcctctttcc
     1141 accattatac aggataaaag cctcccttgt caaagggagg tggacgccgt aggcggccgg
     1201 ggggattctg acgggccgtg ccatagctgc tcgtcacacc cgcagccggg caggaatccc
     1261 tccgccttcg ctaacgctcg gcacctccct ttgacaaggg aggctttatt tagaccttcc
     1321 ccgcccgact cgcggctgtc tttttgtgca gctgttccga cagtaccacc atcagcaaca
     1381 gcatggcgag cagtgcccat gggtagagct gcatcgtgat attctgcgct aacacgccga
     1441 aaaacggcga cagcagcgtc acgccaacgt aggcggcggc catctggatg ccggtcatcg
     1501 ccatgctcag gtttttgccg aagttggcgg gcgtctcgtg gatgatgcag gggtagatgg
     1561 gcgcgcagcc cagtccgatg accacaaggc ccaccggcag cgcagcgctg cccggcagaa
     1621 gcagcagcgc aatacccacg gcgatgagtg tctgccccag acggatcatc tggtggtcgg
     1681 tgaaccgcat cgtcaggaag ccgctcaaaa accggccgcc cgtgatgccc agatagaaca
     1741 ggcttgccca gctggcggca gtcgtagcat ccaaaccgcg taccagtgtg cagtagctgg
     1801 ccgcccacat gccggccacg gcctccaaac cgttgtatag cgcaaagcag cacatcacct
     1861 ccggtacgcc gggcactttc atcagctgcg gaagggtgcg atgctcgggg gtgaagtcct
     1921 cccctgctcc ggcatcggcg ggggtctgcc acagcggcag gctgaatatc agcacagccg
     1981 tcaagacgat ttgcagaatc gagatgatgc ggtagccgtt ctgccaggtg ctgcccgcca
     2041 atgcccagcc catgatgacc gggccgcccg ccgcgccgat gccccacata cagtgcagcc
     2101 agctcatgtg gcggctctcg tagtgcaagg ccacatagtt gttcagtgca gcgtctacgc
     2161 tgccggcgcc gaggccgtaa ggaatggccc acaaacacag ctgccagaac tgtgtgcagg
     2221 tggaaaagcc gaacagtgcc acggcggtgg tggccacgct cagcgccgtg acccgtccgg
     2281 tgccgaggcg ggtgttgagc cgctcgctgg caaggctgga cacgatggtc ccggcggaaa
     2341 tgatcatcga tacgccgccc gcccaggcga cgcccgcgcc cagctgcggg tacatgctgg
     2401 gccacgccgc acccagcagc gcgtccggca ggcccaggct aatgaaggcc agatagatga
     2461 tgggcagaag cagagaaacc atagtaaata cctcttgact ttttgtgatt gcgatactat
     2521 gattatatca tcaaacggca ggaaataaag gcactattcc cgtggaattc tggtataatt
     2581 tcgccaaaaa tggagatgaa cagaagatgg cactgtctgt atgcagaacg gtcatagacg
     2641 attttgcccg ggaactgctg aaacatggca cggcagcgtt ccccatcgcc tgctaccatg
     2701 acgatttgca gaccgagccg gtcccttggc attggcatga ggaactggaa gtgctggtgg
     2761 tcagcgaggg cacgatcctg gccactgccg ccggggaaaa ataccgcctg gaaaagggcg
     2821 aggggctgtt catcaacgcg ggcgtgctgc acggcgactg gccgctgaac gctgggccat
     2881 gccgcctgca cagcatggtg ttccacccgc ggctggtggg cggcagcccg gacagcgttt
     2941 tttggcagaa atatttgcag ccgctgctga ccgaccccag ccgcccctgc gccgtgctgc
     3001 gtcccggtgt ggaatggcag cgtgaggcga tgaaccaaat ggagcgggcg ttccgcgcgg
     3061 tggtcaacga gcagcccggg tacgagttca aggtccgcgc ggcgctgttg gaaatgctgt
     3121 tccagctggt gggccacgcc ccggcagtgc aggcgctgcc gaaaaaagcc ctgcgcagca
     3181 ccgaccgcat caagacgatg ctgcaattca tccaggagca ttacgctgag gacgtgtcgg
     3241 tggagcagat cgcggccagc gcggccatca gccccagcga gtgcctgcgg tgcttccatg
     3301 acatgatcgg cacaacgccg aaccaatacc tgagggacca gcgcacccag cgtgccgctg
     3361 agcttttgtg cggcacgggg ttacaggtgt cggaaatcgc ggcgcagtgc ggtttccagg
     3421 atgccagcta ttttgcccgc gcgtttcggc aggtctacgg ctgcggaccg acggagtaca
     3481 ggagaaaggc aaggagagcg ggagcggtgt agcgggaaaa gcccctcagg ccggttttgc
     3541 ggccggaggg ggctttggca cattgccgct gttaccaata aaaaataccg ctgtcaattt
     3601 cctgacagcg gtatttttat aaaccgatat tagccgttat tcacgcggtt cttgatttca
     3661 tcaacaacct tggcgatgaa ggcatccagc tccatcgtcg tggtctcgcc cttgcggtcg
     3721 cggacgctga tgttgttggc ctcgacttcc ttcgcgccca gaaccagcat atacggtacg
     3781 cggtcaacct gctgggcctg gcggatcttg tagccaatct tctcgttgga ctcatccagc
     3841 acaacgcgga caccggcggc ctgcagcttg gcggccacgt ccttggcgta gtccagcgtc
     3901 ttttcgctga ccggcagcac cttgacctgc gtcggggcca gccaggtcgg gaaacgtccc
     3961 ttcgtctcct caatcaggta ggccatgaaa cggtcaagag agccgaggat ggcgcggtgc
     4021 agcacgacgg gggtcttttc gctgccgtcc ttgtccacat aggtcaggtg gaacttggcg
     4081 ggcaggcaga agtccagctg gcaggtggac agcgtgtact cggcaccgac ggcgggcttg
     4141 acgttgacgt ccagcttcgg gccgtagaac gcggcctcgc cgatctcctc ggtgaagtgg
     4201 atgcccagct cggtcaggac ctcgcgcagg gcgttctcgg catggttcca catagcgtcg
     4261 tcgtcatggt atttcttctt gtcagcgggg tcgcgcaggg acagaacaca gcgataatcc
     4321 gtgatgttga agtccttgta gacgtcgaaa atcagggcgc agacattggc aacttcgctc
     4381 ttgatctgct cgggcgtgca gaacaggtgg gcgtcgttct ggcagaagtg gcggccgcgc
     4441 tcgatgccct tcagcgtgcc ggaggactca tagcggaagt cgtgggcaat ctcgccgatg
     4501 cggataggca gctggcggta gctgtggggc tggttggcgt aaatcatcat gtgatggggg
     4561 cagttcatgg ggcgcagcac gtagctctca ccctccatct ccatggcggg gaacatattc
     4621 tcgcggtagt gatcccagtg accggaggtc ttgtacaggt tcaccgtgcc gatgcagggg
     4681 gccatgacgt gcaggtagcc gcgctcgcgc tccttgccct tgatataatc ttccaactgc
     4741 tgccagacgg tgtaaccgtt aggcaggaac atcggcaggc cgcggccgac gaggtcgtcg
     4801 gtcatgaaca ggcgcatctc cttgccgatc ttgcggtggt cgcgctcacg ggcctcctgc
     4861 tgctgcttct cccacgcgtc cagctcctcc tgattgcgga aggccacgcc gttgatgcgg
     4921 gtcagcatct tgttctcttt gtcgttcttc cagtacgcgc cggactgctg ggtgatttta
     4981 aacgccttca gcgccttggt gtaggtcagg tggggtccga tgcacatatc aacgtactcg
     5041 ccctgctggt agaagctgaa ctcggtctcg tcggccagat cggccatatg ctcgatcttg
     5101 tagtgctcct ggcgctcctc catcagcttg acagcctcgt cgcggggcag gatgaacggc
     5161 ttgatgggaa gattctcctt gcagatcttc ttcatctctt tttcgatggc ggccaggtcc
     5221 tcgtcagtca gcttggtgtc gcccagatca acatcgtaga aaaagccgtt ctcggtggcg
     5281 gggccgaagg caaagtcagc ctgcgggtag aggcgcttga tggcctgggc catgatgtgg
     5341 gctgccgtgt ggcgcagaac gtgcagttct tcttcctgcg ggcactcggc aacggtgccg
     5401 tccttgtaga tgatcttcat gattgtacgc tccttttgca tttgtgcaat aaaaaacgct
     5461 ccatccctca cattcaaaaa gggatgaagc ggtaagctcc acggttccac ccaaattgcg
     5521 cttatatata aggtaagcgc cgctcgtgtc ggctgtaacg ggccgtaccc ggcggaggtt
     5581 cgcccccgcg ctcggcagtg gtaaggctgc gggccgctgt aaaaccgctt gcaccacgca
     5641 acgtttgctg cggcggtcgt ctctgataca ggaccctgca tcccggttgt ctgcctcatg
     5701 gctttgtaca ggatgaagtt aagttattta tagcacaagt gcggctttaa gtcaagtaga
     5761 aaaatttgga atgttgaggc tgtaggggcc gcacatgtgc ggcccgcaac gtaaaggcaa
     5821 gcaggcgtct gtcataaagc tgcgggccgg gcatgcccgg cccctacatt tatacctccg
     5881 ccagattgca caaaaaactt tccactcccc tgctctgccc atgtaaaacg ccgaagttat
     5941 taaatataca ttgactttac atatttatgc agttatactg tatacatcaa ccaagtgagg
     6001 gcttggctgt gtacctattt ttacaaatac agaaatagag ggaattcaca tgaaaaagct
     6061 cactgctctg atgctcagct ccgctatgat gctgagcctg gccgcctgcg gcggttccgc
     6121 ttctaccgat actgcttcct ccgaggctgc atcttctgag gctg
//
LOCUS       DBMDSQ010000099         6129 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_3260_length_6129_cov_1.764764, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000099 DBMDSQ010000000
VERSION     DBMDSQ010000099.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 6129)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6129)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6129
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3260_length_6129_cov_1.764764"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1308)
                     /locus_tag="ACZPAX_04945"
     CDS             complement(<1..1308)
                     /locus_tag="ACZPAX_04945"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007045843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR1113278.1"
                     /translation="MPENAKPNNERPADLAVQDSVGGMARRLLNPAHLRDLAGRSVRT
                     LREQGAEQLWRDVKFRVGLAMHHDDWRHRADIPLRRELKAQRAAHLQGPKVSIIVPLY
                     NTPPKFFDEMLQSVVKQTYTNWELVLVDASDADKRLESRLPKAVPGIIVYEPIENGGI
                     AANTTRGFELATGDFIALLDHDDVLYLNALFEVVQTIQNTGADFVYSDEIVLSADLKE
                     LGGYHFKPDFAPDYLRGVNFITHLAVFSRPLLDAAGAYESKEFDGAQDHDLILRLTEK
                     AQRIEHIKKVLYIWRGHAGSTAAGMEAKPYAIAAGERAIDAQLERLSLPGHAMAVPDA
                     PGAFQVKYELTGHPKISVLIPNKDHTDDLDRCLTSLYKNAGYDNFEAIVIENNSTDPA
                     TFAYYKTLHDRFPTCRVVTFEGKGPFNFSAINNFGATFAEGEHL"
     gene            complement(1312..2088)
                     /locus_tag="ACZPAX_04950"
     CDS             complement(1312..2088)
                     /locus_tag="ACZPAX_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007045842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1113279.1"
                     /translation="MAEQKPIISIDHVSMRFNLAKEKHESLKEYFVALLHGGVRFDEF
                     FALDDVSFDIMPGDFYGLIGLNGSGKSTLLKVISGVYKPSAGRVTVNGTIAPLIELGA
                     GFDMDLTARENIYLNGTVLGYTPKYIDSKFDDIVEFSELQDFLDVPLKNYSSGMVARL
                     AFAVATMTKPDILIADEILSVGDFLFQEKCEKRMAELLSGGTTVILVSHSIDQIERMC
                     NKVAWLDHGHLRRLGPTKEITAEYRRFEKKDAMPAKVTEE"
     gene            complement(2101..2898)
                     /locus_tag="ACZPAX_04955"
     CDS             complement(2101..2898)
                     /locus_tag="ACZPAX_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007045841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1113280.1"
                     /translation="MQNLKNTFAGFWKYRYLLQNLITRDFKLKYRRSVLGVAWSVLNP
                     LLTCLVMWAVFGALFNQRGQGIEDFPLFLIIGQLMFNFFRESTTMAMESVLKNGPLLR
                     KVYIPKYIFPLEKCCFALVNFFFSLVALFIVALLTWSHISVKTVLLAVYPIVMLFIFS
                     LGIGLILSTLYVFVRDIMHIWEVFCTLLVYGSAIFYDPRQMESWMQYIINLNPIYWYI
                     TSVRSCVMWGEGLTPNMVLIPFLCAALSLGLGVCVFKKNQDKFVLYM"
     gene            complement(2908..3474)
                     /gene="rfbC"
                     /locus_tag="ACZPAX_04960"
     CDS             complement(2908..3474)
                     /gene="rfbC"
                     /locus_tag="ACZPAX_04960"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015565270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HIR1113281.1"
                     /translation="MGKFNFIKTEIPEVQIIEPTVFGDDRGYFMETYQIDEFAAAGID
                     KPFVQDNQSRSTKGVLRGLHFQTKHTQGKLVRVTLGEVFDVAVDCRPNSKTFGKWVGV
                     TLSAENKKMLWIPEGFAHGFVVLSDVAEFCYKCTDVYDPTAEGGIPYDDPTVNVQWPD
                     CGCAHKTSAKDKEHTPFAEQKFEYFEKY"
     gene            3673..4227
                     /locus_tag="ACZPAX_04965"
     CDS             3673..4227
                     /locus_tag="ACZPAX_04965"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HIR1113282.1"
                     /translation="MTKREARAQARTLRAALDMQAVGRGMAEVLFPLPCWAAAGTVLA
                     FAAMPDEPDTAAILRQALADGKHLLLPRVLSKTAMDWVEIPDLALLRPGAFGILEPPA
                     DLPTADPAVFARPLALIPCLAAGRDGVRLGRGGGYYDRFLAHYKGAKLLLCPEAVLTD
                     TLPCDDWDAHFAPEEILTEKGIQV"
     gene            4224..4901
                     /locus_tag="ACZPAX_04970"
     CDS             4224..4901
                     /locus_tag="ACZPAX_04970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113283.1"
                     /translation="MKKLLFATALLAALLLSACGGKTNDDDFMNKPAVTPEPGMEIDP
                     EFGVDPEPETDENAQLAPDAELSDMVDALYKIQPVELMGMETTAIDLTDETWYGYLAG
                     LTADNVGKVDAAVISEPMTGSQAYSLVLLRLRDKADAREVADSMEENISMRKWVCVEA
                     DKARVVSFDDKRLYVMADSELVDVDLLADAATKAFNATFDVDDSLVNEDESELPPELL
                     SAPAVAD"
     gene            complement(4967..6067)
                     /gene="acrA"
                     /locus_tag="ACZPAX_04975"
     CDS             complement(4967..6067)
                     /gene="acrA"
                     /locus_tag="ACZPAX_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007045836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016628 - oxidoreductase activity, acting
                     on the CH-CH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acryloyl-CoA reductase electron transfer subunit
                     beta"
                     /protein_id="HIR1113284.1"
                     /translation="MAEFNAMDTAAFKGVWVFCEQREGEIQSISYQLLSEGRKLANDL
                     GVELAGVVMGSGLAEADLKTLGGYGADRVYNIDSPLLKDYTTDGYAKALCDLIMDKKP
                     EIMLIGATNLGRDLGPRCAARLHTGLTADCTHLDVDVEKYKNFLRTTSNIDVDNTKFE
                     ENTNLKMTRPAFGGHLMATIICPRFRPQMSTVRPGVMQMQAFDQAGADKVVIENVAPA
                     LTADDIHVQVLDIKKSAEKLVDLIGADVVVSVGRGISADPEKGIKLAEELAEALGGVV
                     GASRAVTDEGWLSADHQVGQTGKTVHPRIYVALGISGAIQHVAGMQDSENIIAINKNE
                     NAPIFDVATYGIVGDLYKVVPELIKEIKAAKA"
BASE COUNT         1243 a         1733 c         1861 g         1292 t
ORIGIN      
        1 caggtgctca ccctcggcaa acgtcgcgcc aaagttgttg atagccgaga aattgaacgg
       61 gcccttcccc tcaaacgtca cgaccctgca ggtcgggaac cggtcatgca gcgttttata
      121 gtaggcgaat gtcgccgggt cagtggagtt gttctcgatg acgatggcct caaaattgtc
      181 atacccggcg tttttgtaca gacttgtcag gcagcggtcc aggtcgtcgg tgtggtcctt
      241 gttgggaatc agcacgctga ttttcgggtg gccggtcagc tcgtacttga cctggaacgc
      301 gccgggtgca tcaggcaccg ccatggcgtg ccccggcagg ctgagacgtt cgagctgcgc
      361 gtcgatggcc cgctcccccg ccgcgatggc gtagggcttg gcctccatgc ctgcggcggt
      421 ggaacctgcg tggccgcgcc agatgtagag cactttcttg atatgctcga tgcgctgtgc
      481 tttttcggtc aggcgcagaa tcaaatcatg atcctgtgcg ccgtcaaact ctttcgattc
      541 ataggccccg gcggcatcca gcagtgggcg gctgaacacg gccagatggg taatgaagtt
      601 gaccccgcgc aggtagtccg gcgcaaagtc gggcttgaag tggtagccgc ccagctcctt
      661 caaatcggcg gagaggacaa tttcatcact gtacacaaaa tctgcaccgg tgttttggat
      721 ggtctgcaca acctcgaaca gtgcgtttaa atacagtacg tcgtcgtggt ccagcagggc
      781 aataaaatcg ccggtggcca gctcgaaccc acgggtcgtg ttggccgcaa tgccgccgtt
      841 ctcaatcggt tcatagacga tgatgccggg cacggccttg ggcaggcggc tttccagccg
      901 cttgtcggcg tcgctggcgt ccaccagcac caattcccag ttcgtgtagg tctgtttgac
      961 gacactttgc agcatttcgt caaaaaattt cggcggcgtg ttgtacagcg ggacgatgat
     1021 gctgaccttc ggcccctgca aatgtgcagc gcgttgggct ttcagctccc ggcgcagcgg
     1081 gatatctgcc cgatgccgcc agtcgtcgtg gtgcatggcc agcccgacgc gaaatttcac
     1141 gtcgcgccac agctgctcgg ccccctgctc ccgcaaggtg cgcacactgc gcccggccag
     1201 atcccgcagg tgggcagggt tcaaaagccg ccgcgccatg ccgcccacgc tgtcctgtac
     1261 ggccaggtcg gcggggcgct cgttgttcgg ttttgcattt tcgggcatcg tttattcctc
     1321 ggtaactttc gccggcatgg cgtccttttt ctcgaacctg cggtactcgg cggtgatctc
     1381 cttcgtcggg cctaagcggc gcagatggcc gtggtcgagc caggcgacct tgttgcacat
     1441 acgctcgatc tggtcaatgg agtggctgac caaaatgacc gtggtaccgc cggaaagcag
     1501 ctccgccatg cgcttttcgc acttttcctg aaacaggaaa tcgcccaccg acagaatctc
     1561 gtcggcaatc aggatatcgg gcttcgtcat cgtcgccacg gcgaaggcca aacgcgccac
     1621 catgccggag gagtagtttt tcagcggtac gtcgagaaaa tcctgcaatt cgctgaactc
     1681 gacgatatcg tcaaattttg agtcgatgta cttgggcgtg tagcccagca ccgtgccgtt
     1741 taaatagatg ttctcccgcg cggtcaggtc catgtcaaag cccgcgccca actcgatcag
     1801 cggcgcaatc gtgccgttga ccgtgacgcg gcccgcgctg ggcttataca cgccggaaat
     1861 gactttcagc agcgtggact tgccgctgcc gtttaagcca atcaggccgt agaaatcgcc
     1921 gggcatgata tcaaacgata catcgtccaa agcgaagaac tcgtcaaagc gcacgccgcc
     1981 gtgcagcagc gccacgaaat actctttcag gctctcgtgc ttttctttgg caaggttgaa
     2041 gcgcatcgaa acatggtcga tggagataat gggtttctgc tctgccattt cgatgccccc
     2101 ttacatgtac aggacgaatt tgtcctggtt ctttttaaag acgcagacgc ccagccccag
     2161 gctgagtgcg gcgcagagga acggaatgag caccatgttc ggggtcagcc cctcgcccca
     2221 catgacgcag gaacgcaccg aggtgatgta ccagtagatg gggttcaggt tgatgatgta
     2281 ctgcatccag ctttccatct ggcgcgggtc gtagaagatg gcgctgccgt agacgagcag
     2341 ggtgcagaac acttcccaaa tgtgcatgat atcgcgcaca aaaacgtaga gcgttgacaa
     2401 aatcagcccg atgcccaggc tgaaaataaa cagcatgacg atgggataga cggccagcag
     2461 tacggtcttg accgaaatgt gcgaccaggt cagcagcgcc acgatgaaga gcgccaccag
     2521 gctgaagaag aagttgacca gcgcaaagca gcacttttcc agtgggaaaa tgtacttagg
     2581 gatgtacact tttcgcagca atgggccgtt tttcagcacg ctctccatgg ccatggtcgt
     2641 actctcgcgg aagaagttga acatcagctg gccgatgatg aggaacagcg ggaaatcctc
     2701 aatgccctgg ccgcgctggt tgaacagcgc accgaacacg gcccacatga ccaggcaggt
     2761 cagcagcggg ttcagcacgc tccaggctac gcccagcacg ctgcggcggt atttcagctt
     2821 gaagtcgcgg gtgataagat tttgcagcag gtagcggtac ttccagaacc ctgcaaaggt
     2881 gtttttcaga ttctgcacag cttatcctca atatttttca aaatactcga acttctgctc
     2941 ggcgaagggg gtgtgctcct tgtccttggc gctggtcttg tgcgcacagc cgcagtcagg
     3001 ccactgcacg ttgacggtcg ggtcgtcgta cgggatgccg ccctcggcag tcgggtcgta
     3061 gacgtcggtg catttgtagc agaactcggc cacgtcagac agcacgacaa agccgtgggc
     3121 aaagccctcg ggaatccaga gcatcttttt attctcggcg gacagcgtca cgcccaccca
     3181 cttaccaaaa gtcttgctgt tggggcggca gtccacggcg acgtcgaata cctcgcccag
     3241 ggtcacgcgg acaagcttgc cctgggtgtg cttggtctgg aagtgcagac cgcgcagcac
     3301 gcccttggtg gagcggctct ggttgtcctg cacgaagggc ttgtcgatac cggcagcggc
     3361 gaactcgtcg atttggtagg tctccatgaa atagccgcgg tcgtcgccaa agacggtcgg
     3421 ctcaatgatc tgcacttcag gaatctcagt cttgataaaa ttaaacttgc ccatggttgg
     3481 aatctccttt ttctatattt cataggacag tatatacata gcgatacagg ttatattata
     3541 ctccattata gcgcattggg caagatggca gtaaaatttt gtcgctgctg taacaacacg
     3601 tccgcctttt ccgtcttgta gggtgagggc agatgtgcta tgataatacg aaatgaaagg
     3661 atggtgcggc agatgacaaa acgcgaagcc cgcgcacagg ccaggaccct gcgtgcagcc
     3721 ctggatatgc aggccgtggg gcgcggcatg gcagaagtgc tctttcccct gccctgctgg
     3781 gcggcggcag gcacggtgct ggcctttgca gccatgccgg atgagccaga tacagcggcc
     3841 attttgcgcc aggcgctggc ggacggcaaa cacctgctgc tgccccgcgt gttgagcaaa
     3901 acggccatgg actgggtaga gatcccggat ttggctctgc tgcgtcccgg cgcttttggg
     3961 attttagagc cgcccgccga cttgcccact gccgatccgg cagtctttgc gcggccgctg
     4021 gcgctgatcc cctgcctggc tgctggccgc gacggtgtgc gcctggggcg cggcggaggg
     4081 tattatgacc gctttttggc acactataaa ggggcaaagc tattgctctg ccccgaagcg
     4141 gtgctgacgg ataccctgcc ctgcgacgat tgggacgccc attttgcgcc cgaagaaatt
     4201 ttgaccgaga aaggaataca agtatgaaga agctgctttt tgcaacagcc ctgctggccg
     4261 cgctgctttt gagcgcctgc ggcggcaaaa ccaacgacga tgattttatg aacaagcccg
     4321 ccgtgacgcc cgaaccgggc atggagatcg acccggaatt tggtgttgac cccgagcccg
     4381 aaaccgatga aaatgcccag ctggcaccgg acgccgagct gagcgatatg gtggacgcgc
     4441 tctataagat tcagccggtg gagctgatgg gcatggagac aacggccatc gacctgaccg
     4501 acgagacctg gtacggctac ctggccgggc tgaccgccga caatgtgggc aaagtggacg
     4561 cggctgtcat cagcgagccg atgactggca gccaggcgta cagccttgtg ctgctgcgcc
     4621 tcagggacaa ggccgatgcc cgtgaggtcg ccgattcgat ggaggaaaat atcagcatgc
     4681 gcaaatgggt ctgcgtggag gcagataagg cccgcgtggt cagctttgat gataagcggc
     4741 tgtatgtcat ggcagacagt gagctggtcg atgtcgatct gttagcggat gctgctacaa
     4801 aggcctttaa tgcgacattc gatgtcgatg attccctcgt caatgaggac gagagcgagc
     4861 tgcccccgga gctgctgtcc gccccggctg tggcagactg agcgatacaa aaaaacaaag
     4921 agccgtgccc tttgcggggc acggctcttg ttgtttacag gcgggatcaa gccttggcgg
     4981 ccttgatctc cttgatgagt tcgggaacga ccttgtacag atcgccgacg atgccgtagg
     5041 tggcaacgtc gaagatgggg gcgttctcgt tcttgttgat ggcgatgatg ttctcggagt
     5101 cctgcatacc ggcaacgtgc tggatggcac cggagatgcc cagggcaacg tagatgcggg
     5161 gatgcacggt cttaccggtc tggccgacct gatggtcagc agacagccag ccctcatcgg
     5221 tgacggcacg ggaagcaccg acaacaccgc ctagagcctc ggccagctcc tcagccagct
     5281 tgatgccctt ctcggggtca gcggagatac cgcggccgac ggagacgaca acgtcagcac
     5341 cgatcaggtc gacgagcttc tcagcgctct tcttgatgtc gagcacctga acatggatgt
     5401 cgtcggcagt cagagcgggc gcaacattct cgatgacgac cttgtcagca ccggcctggt
     5461 cgaacgcctg catctgcatg acgccggggc ggacggtgga catctgcggg cggaagcgcg
     5521 ggcagatgat ggtagccatc aggtggccgc cgaatgcggg gcgggtcatc ttcaggttgg
     5581 tgttttcctc gaacttggtg ttatccacat caatgttgga ggtggtgcgc aggaagttct
     5641 tgtacttctc gacatcgacg tccagatggg tgcagtcggc ggtcaggccg gtgtgcagac
     5701 gggctgcgca gcgggggccg aggtcacggc ccaggttggt cgcgccgatc agcatgatct
     5761 cgggcttctt gtccatgatg aggtcgcaca gcgccttggc atagccatcg gtggtgtagt
     5821 ccttcagcag ggggctgtcg atattgtaaa cacggtcagc gccgtagccg cccagagtct
     5881 tgaggtcagc ctcagccagg ccgctgccca tgacaacgcc agccagctca acgccaaggt
     5941 cgttggccag cttgcggccc tcgctcagca gctgatagct gatggactgg atctcgcctt
     6001 cgcgctgctc gcagaaaacc cacacgccct tgaaggcggc ggtgtccatt gcattgaatt
     6061 cagccattgt tcgtttctcc ttcctcaaat caggtgcttg tcgttcagga tgccgaccag
     6121 ctgggcagc
//
LOCUS       DBMDSQ010000100         6120 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_3269_length_6120_cov_1.739824, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000100 DBMDSQ010000000
VERSION     DBMDSQ010000100.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 6120)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6120)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6120
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3269_length_6120_cov_1.739824"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(10..1314)
                     /locus_tag="ACZPAX_04980"
     CDS             complement(10..1314)
                     /locus_tag="ACZPAX_04980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113285.1"
                     /translation="MKKRIIKIAAFLVGFLLINLLLCGFMPPDNGSSLAMWRNYHSKD
                     SVDIAVIGSSLASCALPEEELAAATGKSVALMATNAQSWDMSKIALESVLREHTPQYV
                     ILVMDLYNMTGEPYPKAQKAFLSAELQTSRWQDRPAELLRYMTSADRFTDADSLNALF
                     PWQGGNWPTDWPATIRQKWNALTARIAARGAAHGQPEGDTVINFDTVGNVNTWNQTNH
                     PFTQQHIDELVSMMQLCQEKGVPLLVISAPKTTLDVISYDTYFDDYAFFKKLAGLYGA
                     SYYDFNFAKQELFANVEPDYYKDFTHMNTTGGHAFSQCMAKFLALLDEGADVDALFET
                     PEEYLAAVNYITNVYLTPEIQTDKILLLAGSYHGPSVEAEYEFWVKAPGETDYAKISD
                     YDTRTWVEYPTTVHGSYQFRVNARVVGSYADFERYYECYLDF"
     gene            complement(1481..3886)
                     /locus_tag="ACZPAX_04985"
     CDS             complement(1481..3886)
                     /locus_tag="ACZPAX_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013483708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HIR1113286.1"
                     /translation="MQIREVFDRKRFVFLDGGMGTQLQARGLQPGQKPELAALEMPDV
                     LTAIHTDYANAGADILLANTFGANAKKLAGCGHTVEDVVTASIACARKAADTTGALVA
                     LDIGPLGELLVPAGTLSFEDAYAEFAQVIRAGAAAGADLVFLETMTDLYELKAAILAA
                     KENCALPIFTSMSFESRGRTFTGCTVESYAVTAAGLGADAVGINCSLGPKEILPFAQR
                     LCRSVPAGVPVFVKPNAGLPNPDGSYNLDPDGFAAEMKEYAAIGVSMVGGCCGTTPAF
                     IAKLHVTFSPLAPADKISIRRSCLCTPVRFVEVNGITVVGERINPTGKKRLQQALRDG
                     DSAYPCTQAVAQAEAGAQVLDVNAGLPGIDEAATLEQLVKDLQAVTDLPLQLDSSNPE
                     ALSRALRIYNGKPIVNSVNGETETLEKILPLCKKYGAAVVGLALDKGGIPPTVEGRVA
                     IARRIVDAAHAAGIPDEDIYIDCLCLTASAQQEGATQTLQALARCKKELGVRTVLGVS
                     NISFGLPCRGYLNTTFLTMAMSAGLDLAIMNPNTPEMMAAVRAYRVLTCQDPQSTDYV
                     AAYADVQIQTTQTSKSAATVAEVSAAAPGGDALFEAVRRGLKAEARAAADAALTMREP
                     LDVVNTSLIPALDAVGDGFEKGTVFLPQLLQAATAAQAAFEAIKAKIAASGQAQGSKG
                     KIVIATVKGDVHDIGKNIVRVILENYGYDVLDLGRDVDPERVVEAVRQTGAKLVGLSA
                     LMTTTVPNMQATIEALHAANLDCKVMVGGAVLTPDYARDIGADYYCKDAKASADLAKQ
                     LLG"
     gene            complement(3898..4566)
                     /locus_tag="ACZPAX_04990"
     CDS             complement(3898..4566)
                     /locus_tag="ACZPAX_04990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HIR1113287.1"
                     /translation="MERRKPAAIRRATALRYMGASGWTPDDATTALLDKAEKQILAIA
                     APHVVWRVLPLAVLPFDNCGTDLARHLQGCDKALLMAATLGSEIDKFLRRLELTDIAS
                     ATAADALASVLLEQVCDEWEAEQRPLFEAKGLYLTGRYAPGYGDCPLDLNDELCLAVD
                     SVRGCGLAVTPQHLLTPRKSTTAILGIADHPVTGTRAGCATCHLKETCSFRKRGTTCF
                     NQEG"
     gene            complement(4566..5429)
                     /locus_tag="ACZPAX_04995"
     CDS             complement(4566..5429)
                     /locus_tag="ACZPAX_04995"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase [NAD(P)H] activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HIR1113288.1"
                     /translation="MTITEQFKTKRCVFSIECFPPKQTTQMDKLKDTLQKMQALTPGF
                     ISVTFGAGGSAGGVSTAEVADYIQNELHIPTLAHLICMGNDETCAAEILDQLEAVGVR
                     DVLALRGDRSPARPESPDFKHASDLTSFIKWKKPNFSVHGACYPEGHPEADTLLHDVE
                     NLRIKQAAGAEHLLTQLFFDNMHFYRFLNLARRAGITLPVSAGVMPIVKRSQIERTVA
                     LSSASLPSDFTRMISRYQDDPASLYDAGIDYAVNQLRDLIEGGADGIHLYAMNDAAVA
                     TKVYDGIRDLL"
     gene            complement(5446..>6120)
                     /locus_tag="ACZPAX_05000"
     CDS             complement(5446..>6120)
                     /locus_tag="ACZPAX_05000"
                     /inference="COORDINATES: protein motif:HMM:NF012955.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HIR1113289.1"
                     /translation="LDALEVETLDYLVLTHPHADHTGNARAVLQNHPVQTLLLPDWQP
                     GPEDTADWPRNLAQAAADSGAEILTTEAGDSYSLGGGTLQILQGGSAEAADDSVNNAS
                     LCTLFTAGDFRFLDTGDAEEAAEQQLLDTYGTSLHTTLFKAGHHGSYTSNSLAFLQAA
                     APQAVAVSCGLNNDYGHPHRAALQNFAAAGAEVYRTDLQGSLTFIWQNDTLTVETTAD
                     SAELAA"
BASE COUNT         1199 a         1750 c         1923 g         1248 t
ORIGIN      
        1 tccgtatatt taaaaatcaa gataacattc gtaataacgc tcaaaatctg cataagaccc
       61 gactacccgc gcattgacgc ggaactgata gctaccgtgt actgttgtgg ggtattcgac
      121 ccaggtacgg gtgtcgtaat cggaaatttt tgcgtagtcc gtctcaccgg gggctttgac
      181 ccagaactca tactcagcct cgactgaggg accgtggtag gaacccgcca gcagcagaat
      241 tttgtccgtt tgaatctccg gcgtcaggta cacatttgtg atatagttga cggcggccag
      301 gtattcttcc ggcgtctcaa acagggcatc cacgtcagct ccctcgtcca gcagggccag
      361 gaactttgcc atgcactggc tgaaagcgtg gccgccggtg gtgttcatgt gggtgaaatc
      421 cttgtagtaa tccggttcaa cgttcgcgaa cagctcctgt ttggcaaagt tgaagtcata
      481 gtaagatgcg ccgtaaagcc ctgccagctt tttgaaaaag gcgtaatcgt caaaataagt
      541 atcgtagctg atgacgtcca gcgtcgtttt tggggcgctg atgacgagca gcggcacgcc
      601 tttctcttgg cagagctgca tcatcgagac aagctcgtca atgtgctgct gggtgaatgg
      661 gtggttcgtc tggttccaag tattcacatt gccgaccgtg tcaaaattga tgacggtatc
      721 cccttccggc tggccgtgcg ccgcgccgcg cgcagcaatg cgggcagtca gggcgttcca
      781 cttttgccgg atggtggcgg gccaatcggt gggccagttg ccgccctgcc aggggaacag
      841 cgcgttcagc gaatccgcgt cggtaaagcg gtcggcgctg gtcatgtaac gcagcagttc
      901 ggcggggcgg tcctgccagc gggaagtctg caactcggca gagagaaaag ccttttgcgc
      961 cttggggtat ggctcccccg tcatattgta caggtccatg accaggatga catactgcgg
     1021 tgtatgttcc cgcagcacgg attccagtgc aattttggac atatcccagc tttgggcgtt
     1081 ggttgccatc agcgccacgg atttgccagt ggcggcggcc agttcttcct ccggcaaagc
     1141 gcaggaggcc agcgacgagc caatgacggc gatatcgacg gaatctttgc tgtgatagtt
     1201 gcgccacatg gcaaggctgg aaccgttatc cggcggcata aagccgcaca aaagcagatt
     1261 gataagcaaa aagccgacaa gaaaagcggc aattttgatg atacgtttct tcaagcagat
     1321 gctcctaaaa atcagacaaa tcagccaatt tacccaaaat gtgcgtattg cttattatag
     1381 cgcataaaac ctgtaactac aatggctttt cctaagaatc agcaattttt acaactgaaa
     1441 acaaaaactg ccgcgtttta gcaaaaaacg cggcaggggg ttatccaaga agctgtttgg
     1501 caaggtcagc gcttgccttg gcgtccttgc agtagtaatc agcaccgata tcgcgggcgt
     1561 agtcaggcgt cagcacggca ccgcccacca tgactttgca gtcgagattc gcggcgtgca
     1621 gggcttcaat cgtggcctgc atgttgggca cggtggtcgt catcagggcg gataggccca
     1681 cgagcttggc cccggtctgg cgcacagcct cgaccacgcg ctcggggtca acgtcgcggc
     1741 ccagatcgag cacgtcatag ccgtaatttt ccagaataac gcggacgatg ttcttgccga
     1801 tatcatgtac atcgcccttg accgtggcaa tgacgatctt gcctttgctg ccctgggcct
     1861 gaccagaggc tgcaatcttg gctttaatgg cttcaaaagc ggcctgggca gcggtcgcag
     1921 cctgcaaaag ctgcggcagg aacacggtcc ccttctcaaa gccgtcgccc acagcgtcca
     1981 gcgcgggaat cagcgaagta ttgaccacat ccagcggctc ccgcatggtc agggcggcat
     2041 cggcggcggc gcgggcctcg gctttcaggc cgcggcgcac agcctcaaac agggcgtccc
     2101 cgcccggcgc ggcggcgctg acttcggcca ccgtggcagc ggatttgctc gtttgggtcg
     2161 tctgaatctg cacatcggcg taggcggcca catagtcggt gctctgagga tcctggcagg
     2221 tcagcacgcg gtaggcgcgc acggcggcca tcatttccgg cgtattgggg ttcatgatcg
     2281 ccagatccag cccggcagac atcgccatcg tcaaaaatgt ggtgttcaag tagccgcggc
     2341 acggcaggcc aaagctgata ttggatacgc ccagcacggt gcgcacgccc agctcttttt
     2401 tgcagcgggc cagcgcttgc agcgtttggg ttgcgccttc ctgctgagcg ctggcggtca
     2461 ggcagagaca gtcaatgtag atatcctcgt ccgggatgcc tgcggcgtgg gcggcgtcca
     2521 caatgcggcg ggcaatggcg acgcggccct ccactgtcgg tgggatgccg cccttatcca
     2581 gcgccaggcc gacgacggca gcaccgtact ttttgcagag cggcaaaatc ttttccagcg
     2641 tctcagtctc gccgttgacc gagttgacga tgggcttgcc gttataaatg cgcaatgcac
     2701 gggaaagagc ctcggggttc gaggaatcca gctgcagcgg cagatcggtg acggcctgca
     2761 aatccttgac gagctgttcc agcgtggcag cttcgtcgat gccgggcaaa ccagcgttga
     2821 cgtccagcac ctgggcaccg gcctcggcct gggccacggc ctgggtgcag gggtaagcac
     2881 tgtcgccgtc acgtaaggcc tgctgcaagc gctttttgcc ggtagggttg atgcgctcgc
     2941 ccacgacggt aatgccgttt acttccacaa agcgaaccgg tgtacacagg cagctgcggc
     3001 ggatggaaat tttatcagcc ggggccagcg ggctgaacgt cacgtgcagt ttggcgataa
     3061 aggcgggtgt ggtgccgcag cagccgccca ccatcgaaac accgatggcg gcgtattctt
     3121 tcatttcggc tgcaaagccg tccggatcca agttgtagct gccgtccggg ttgggcagac
     3181 ccgcattggg tttgacaaag actggcacac cggcgggcac gctgcggcaa agccgctgag
     3241 caaagggcaa aatttccttg gggccaagcg agcagttgat gcccacggcg tccgcgccga
     3301 ggcctgccgc cgtgacagcg tagctttcca ctgtgcagcc ggtgaaggta cggccccggc
     3361 tctcgaagct catcgaggta aagatcggca gggcgcagtt ttctttggca gccaggatgg
     3421 cggctttcag ttcatacagg tcggtcatcg tctccaaaaa gacgaggtcg gcaccggctg
     3481 ccgcgcccgc acggatgacc tgggcgaact cggcataggc atcctcaaag ctcaaggtgc
     3541 ctgcgggaac cagcagctca cctagggggc cgatgtccag cgccaccagt gcgccggtag
     3601 tatcggcggc cttgcgggcg caggcgatgg atgccgtgac gacatcctcc accgtatggc
     3661 cgcagcctgc cagttttttt gcattggcac caaaggtgtt tgccagcagg atatccgccc
     3721 cggcgttggc atagtcggtg tggatggccg tcagtacgtc aggcatttcc agcgcggcca
     3781 gctcgggttt ctgcccgggc tgcagaccgc gtgcctgcag ctgggtaccc atgccgccgt
     3841 ccagaaaaac aaaccgcttg cggtcaaaaa cttcgcgaat ctgcataaga ttctccccta
     3901 tccctcttgg ttgaaacagg tcgtgccgcg cttgcggaac gagcaggtct cttttaaatg
     3961 gcaggtcgca cagcctgcgc gggtgccggt cacaggatga tcggcgatgc ctaaaatggc
     4021 ggtggtgctt ttgcgcgggg tcaaaagatg ctgcggcgtc acagccaggc cgcagccgcg
     4081 aacgctgtct actgccaggc aaagctcatc gttcaaatcc agcgggcagt caccgtagcc
     4141 cggcgcatag cggccggtca ggtacaggcc cttcgcttca aacagcgggc gctgctcagc
     4201 ctcccactcg tcgcagacct gttccagcag aaccgaagcc agtgcatccg cagccgtcgc
     4261 cgaggcaatg tcggtcagtt ccaaacggcg cagaaattta tcgatctccg acccgagggt
     4321 cgcggccatc agcagtgctt tatcacagcc ctgcaaatgg cgggctaggt cggtgccgca
     4381 gttatcaaac ggcagcacgg ccagcggcag gacgcgccac acaacatggg gcgcggcgat
     4441 ggcgaggatt tgcttttccg ctttatccag caacgcggtg gtagcatcat cgggcgtcca
     4501 gccggaggcc cccatgtacc gcagcgccgt ggcgcggcgg atggcggcag gcttgcggcg
     4561 ttccattaca gcaagtccct gatgccgtcg tagaccttgg tggccacggc ggcgtcattc
     4621 atggcgtaca ggtggatgcc atccgcgccg ccttcgatca ggtcgcgcag ctggttgacg
     4681 gcgtagtcga tgccggcatc gtacaggctg gccgggtcgt cctgatagcg ggaaatcatg
     4741 cgggtgaagt ccgacggcag gctggccgag gacagcgcta cggtgcgctc gatctggctg
     4801 cgcttgacaa tgggcatgac gccggccgag acgggcagcg tgataccggc gcgtcgggcc
     4861 aggtttagga agcgatagaa gtgcatatta tcgaagaaca gctgggtcag cagatgctca
     4921 gcacccgctg cctgcttgat gcgcaggttc tccacatcgt gcagcagggt gtcggcctcg
     4981 gggtggccct ctgggtagca ggcaccgtgg acggagaaat tcggcttctt ccatttaata
     5041 aaggaggtca ggtcactggc gtgcttgaag tcggggcttt cggggcgggc cgggctgcgg
     5101 tcaccgcgca gggccagcac atcgcggacg ccgacggcct ccagttggtc aaggatttcg
     5161 gcagcgcagg tttcgtcgtt gcccatgcag atgaggtgtg ccagtgtggg aatgtgcagc
     5221 tcgttctgga tgtagtccgc gacctcggcg gtggaaacgc cgcccgcaga gccgcccgcg
     5281 ccgaaagtca cgctgatgaa gccgggagtc agcgcctgca tcttttgcag cgtgtccttg
     5341 agcttgtcca tctgggtggt ctgcttcggg ggaaagcatt caatcgagaa aacgcagcgc
     5401 ttagttttga actgttcggt gatcgtcatt tatttctcct tattgttacg ccgcaagttc
     5461 ggcggagtcc gcggtggttt ccacagtcag ggtatcattt tgccagataa aggtcaggct
     5521 gccctgtaag tcagtccggt acacctctgc gccggcggca gcaaaatttt gcagcgccgc
     5581 gcggtgcggg tggccgtaat cattgttcag cccacagctg acggccacgg cctgcggggc
     5641 cgcagcctgc aaaaacgcaa ggctgttgga ggtgtaagag ccatggtgcc cggccttgaa
     5701 aagggtcgtg tgcaaggagg tgccgtaggt gtccaagagc tgctgctcgg cggcttcctc
     5761 ggcgtcaccg gtgtctaaaa agcggaagtc acctgctgtg aacagtgtgc agagcgaggc
     5821 attgttgacg ctgtcgtccg cagcctcagc actgccgccc tgtaaaatct gcagcgtgcc
     5881 gccgcctaaa gagtagctgt cccccgcttc ggtggtgagg atttccgccc cgctgtcggc
     5941 ggccgcttgc gctaaattgc gcggccagtc ggcggtgtcc tcggggccgg gctgccagtc
     6001 tggcagcagt agtgtctgaa ccgggtgatt ttgcagcacc gcccgggcat tgccggtgtg
     6061 gtcagcgtgg ggatgggtca gcacaaggta gtcgagtgtt tcgacctcca gtgcatccaa
//
LOCUS       DBMDSQ010000101         6083 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_3292_length_6083_cov_1.885657, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000101 DBMDSQ010000000
VERSION     DBMDSQ010000101.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 6083)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6083)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6083
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3292_length_6083_cov_1.885657"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="ACZPAX_05005"
     CDS             <1..462
                     /locus_tag="ACZPAX_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006574748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HIR1113290.1"
                     /translation="LAGALTFIPYGTMVDHFQHIVYENPELTPAQRHVEWKKLAAIYQ
                     PWMRLDGEIPFYGAGEYWQRQMHIYQSPFYYIDYCLAQTVSLQFWAMLQKDRADAWSH
                     YMAYTKQGGSRTFTELLKNAGLSTPFEESCLRGVSEAAKAWLDSYDLTGIL"
     gene            462..1004
                     /locus_tag="ACZPAX_05010"
     CDS             462..1004
                     /locus_tag="ACZPAX_05010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113291.1"
                     /translation="MADREREKKKKRAYLDDFEKDLNGNYQYRGAHYRYAGEHPHTRV
                     LALLWLFCGGGAALTVGAGALPGATAHAPVYLLLPYMAALVLALYAVVLLVRLTRGGA
                     RVRAYIHEQTAQKLPSLCRATAVLCAAASAGQGVAVFAADAQMLWGMQGVFILFELLS
                     CAAFAAAALFLKRMPWEKEE"
     gene            1342..3639
                     /locus_tag="ACZPAX_05015"
     CDS             1342..3639
                     /locus_tag="ACZPAX_05015"
                     /inference="COORDINATES: protein motif:HMM:NF012706.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR1113292.1"
                     /translation="MKNTKQAIALASAAALSVGMLAGCGGAASSAATASSESNSTATA
                     EASTTAASDGTLVLAETGFESKFSPFFAASAADQDVIDLTQIALLGADRKGEMVLNGI
                     EGETREYNGTDYTYHGPADCVVTENADGTVTYDIKLREDLKFSDGEPVTIDDVIFSMY
                     VFLDPTYDGSVTMYSTPIVGLDEYRSSMTTLSKLIAEAGEDNTDNTKFTAEQQKAFWD
                     AVNDGGVKFAQEIIDYCVESGAAADANDAAGAAAAWNLGELPAGATAKDMFELIGANY
                     DWNFSAMEAETAGTALSDLIPEDVYAYSTTGVNVGDAVASVAGIVKTGDYSMTLTTTE
                     LSTTMIYQLQMPIAPLHYYGDESLYDYDNNSFGFAKGDLSSVRAKTSAPMGAGMFTFS
                     KYSDGVVYLDANPSYYDGAPKVAHVNMKETQEADKITGVQAGTIDISDPSYSLEVADQ
                     IADINGVEGEDGPVITTRLKDYRGYGYIALSAKNVNVGGDPSSQASKDLRKAIMTVIA
                     AYRDEGIDSYYGDTATVINYPVSNTSWAAPSVTDDGYQIAYSTDVDGNEIYTSDMKSE
                     DKYAAALQAALGYFEAAGYTVANGQITAAPAGAKMEYQINIGASGNGDHPSFQTLTNA
                     AAALKTIGFTLTVNDMANASDLFASYQSGAAEGWVAAWQSTNDPDMYQLYHSQGATNY
                     YTINDADLDELIMAARQTTDQEARKAMYKEAMEIILDWGVELPVYQRSEATIFSTERV
                     NIDTIAKDQTPYWTYKSELNNLELN"
     gene            3960..4913
                     /locus_tag="ACZPAX_05020"
     CDS             3960..4913
                     /locus_tag="ACZPAX_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1113293.1"
                     /translation="MRNYIIKRIAQSVLILFCVMFIIYALIRSLPSSFAETMAMQLAQ
                     APGAKPYEEWLAQLNAAYGLDLDIIPGFFTWLSKAVVGNFGDSWKWNVPATQKFTEVI
                     GLSVIMGGISFVLSIVIAVPLGVLAATKQYSRTDYVITAAALVGISLLFSVKLGWFDL
                     YGLVGRDYAQLDAWGQFCDKANHLVLPIATLVIVSIGGYMRYTRTNMLEVLNADYIRT
                     ARAKGLSEHTVIYKHAFRNTLIPLVTIIGGSLPGLFSGALITETLFSIPGIGYISYQS
                     MVAGDIPFTMFYLSFMAVLTLASNLLTDILYGVVDPRVRIS"
     gene            4934..>6083
                     /locus_tag="ACZPAX_05025"
     CDS             4934..>6083
                     /locus_tag="ACZPAX_05025"
                     /inference="COORDINATES: protein motif:HMM:NF024313.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1113294.1"
                     /translation="MSENTQNQNPKQEQYSLNDDRRVKVLSPGALVAKRFFRNRLAVV
                     GLSILIAMFLFSFVGGLVSPYGQDEQFFTYTQMSKEFVGVTRNDTMRFVVADGQNFGS
                     IAQSKALEAVKKGSTEFNYKDTDYTVDIQSDDFYVVYQGSDVMGYASRDLVNEADGAP
                     KFSFDVKLAALTAMTAGESDFTADGVDYTLNKDGEIAANGEQLGYVSRFVVSAADSSV
                     VVTRDFKDCLEEAINENADKFNYTDAEGNEAEYDIVYDASTKVWSVKQMTETYVYDRY
                     ASPSKAHWLGTDTNGMDMLTRLMYGGRVSLIIGFIVVVIEASLGILMGGISGYFGGWI
                     DNIIMRVVDVFYCIPSMPVIIIIGAAMDAMRVDSWKRMLYLMLILGFLG"
BASE COUNT         1238 a         1910 c         1699 g         1236 t
ORIGIN      
        1 ctggcgggtg cgctgacctt catcccctac ggcacaatgg tggaccattt ccagcacatc
       61 gtctacgaga accccgagct gaccccggcc cagcgccacg tggaatggaa aaagctggct
      121 gccatctatc agccctggat gcgtctggac ggcgagatcc cgttctacgg cgcgggtgag
      181 tactggcagc gccagatgca catttatcag agcccgttct actacatcga ctactgtctg
      241 gcccagaccg tcagcctgca gttctgggcg atgctgcaaa aggaccgcgc cgatgcgtgg
      301 agccactaca tggcctacac gaagcagggc ggcagccgca ccttcaccga actgctgaaa
      361 aacgccggcc tgtccacgcc atttgaggaa agctgtctgc gcggcgtcag cgaggcggcc
      421 aaggcgtggc tggacagtta cgatctgacg gggatcctct gatggcggac cgcgagcgcg
      481 agaagaaaaa gaagcgcgcc tatctcgacg actttgagaa ggacctgaac ggcaattatc
      541 agtatcgcgg cgcgcattac cgctatgcgg gcgagcaccc tcacaccagg gtgctggctt
      601 tgctgtggct gttttgcggc ggcggggctg cgctgaccgt cggcgcgggc gcactgcccg
      661 gggccacggc ccacgcacct gtgtacctgc tgctgcccta catggcggca ctggtgctgg
      721 cgctgtatgc ggtggtgctg ctggtgcggc tgacccgggg cggtgcgcgt gtgcgggctt
      781 atatccatga acagaccgca caaaaattgc cctctttgtg cagggcgaca gcggtactct
      841 gcgctgctgc ctccgcgggg cagggggtgg ctgtgtttgc agcggacgca cagatgcttt
      901 ggggaatgca aggtgttttt atactttttg aactgctctc ctgcgctgct tttgccgctg
      961 ctgcgttgtt cctcaagcgg atgccgtggg aaaaagaaga ataattatac attatcataa
     1021 tagagcgtgc tccgcagaaa aacgcagggc acgctttttt ggatgagcag gaaaagtctt
     1081 cccacagggg gaaaaagact ttgggggtta caaaattttc ctctcaaatt cggcaaaagg
     1141 tacaaaaagc aacaaaagtg tgatttatgg ttgacttttt gtcaatttta aggtatctta
     1201 taagctaagt cattatcgac aagtgtacac tgtaaagaga tacagggtgc ggagaggggg
     1261 ttcccccttt ctcgtgttgc gctcttgggg agcacaatat tgatttgtgg gtgagggcaa
     1321 atttatagga ggttttacca catgaaaaac acaaagcaag ccattgcact ggcatctgct
     1381 gccgctctca gcgtcggcat gctggcaggc tgcggcggtg ctgcttcttc cgctgcgact
     1441 gcgtccagtg agagcaacag cacggccact gccgaggcat ccaccactgc cgcttccgac
     1501 ggtacgctcg ttctggccga gaccggcttt gagagcaagt tcagcccctt ctttgctgct
     1561 tctgccgctg accaggatgt catcgacctg acccagatcg ccctgctggg cgctgaccgt
     1621 aagggtgaga tggtcctgaa cggcatcgag ggcgagaccc gcgagtacaa cggcactgac
     1681 tacacctacc acggtcctgc cgactgcgtt gtcaccgaga acgccgacgg caccgttacc
     1741 tatgacatca agctgcgcga ggacctgaag ttctccgacg gtgagcctgt caccatcgac
     1801 gacgtcatct tctcgatgta tgttttcctc gatcctacgt atgatggttc tgtaaccatg
     1861 tactctaccc ccatcgttgg tctggacgag taccgcagct ccatgaccac gctgtccaag
     1921 ctgatcgctg aggccggcga ggacaacacc gacaacacga aattcactgc cgagcagcag
     1981 aaggctttct gggacgctgt caacgacggc ggtgtgaagt ttgcccagga gatcatcgac
     2041 tactgcgttg agagcggcgc tgccgccgac gctaacgatg ctgccggtgc cgccgccgcc
     2101 tggaaccttg gcgagctgcc cgcaggcgca accgcgaagg atatgttcga gctgatcggc
     2161 gcgaactacg actggaactt ctctgccatg gaggccgaga ctgccggtac cgccctgtct
     2221 gacctgatcc ccgaggacgt ctacgcctac tccaccaccg gtgtcaacgt cggtgacgcc
     2281 gttgcttctg tggccggtat cgtcaagact ggtgactaca gcatgacgct gaccaccacc
     2341 gagctgtcca ccaccatgat ctaccaactg cagatgccca tcgctccgct gcactactac
     2401 ggtgatgaga gcctgtacga ctacgacaac aactccttcg gctttgccaa gggcgacctg
     2461 agcagcgtcc gcgccaagac cagcgctccc atgggtgccg gtatgttcac cttctccaag
     2521 tacagcgacg gcgttgtcta tctggatgcc aacccctcct actatgacgg cgcacccaag
     2581 gtcgcccacg tcaacatgaa ggagacccag gaggccgata agatcaccgg cgttcaggcc
     2641 ggcaccatcg acatctccga tccttcctac tctctggagg ttgctgacca gatcgccgac
     2701 atcaacggcg tcgagggtga ggacggccct gtcatcacca cccgcctgaa ggactaccgc
     2761 ggctacggct acatcgccct gagcgccaag aacgtcaacg tcggcggcga cccctcttcc
     2821 caggcctcca aggacctgcg caaggccatc atgaccgtca tcgccgctta ccgtgatgag
     2881 ggcatcgact cctactacgg cgataccgcc accgttatca actaccccgt ttccaacacc
     2941 tcctgggctg ccccgtccgt caccgacgac ggctaccaga tcgcctactc caccgacgtg
     3001 gacggcaacg agatctacac ctccgacatg aagagcgagg acaagtatgc cgctgctctg
     3061 caggctgccc tgggctactt cgaggctgcc ggttacaccg tcgcgaacgg ccagatcact
     3121 gccgctcccg ccggtgccaa gatggagtat cagatcaaca tcggtgcttc cggcaacggt
     3181 gatcacccct ccttccagac cctgaccaac gctgccgccg ctctcaagac catcggcttc
     3241 accctgactg tcaacgatat ggccaacgct tctgacctgt tcgcttccta tcagtccggc
     3301 gctgccgagg gttgggtcgc agcctggcag tccaccaatg accctgatat gtaccagctg
     3361 taccacagcc agggcgctac caactactac accatcaacg atgccgacct ggatgagctg
     3421 atcatggctg cccgccagac caccgaccag gaggcccgca aggccatgta caaagaggcc
     3481 atggagatca tcctggattg gggtgtcgag ctgcccgtct accagcgcag tgaggctacc
     3541 atcttctcca ccgagcgtgt caacatcgac accatcgcga aggatcagac cccgtactgg
     3601 acctacaagt ctgagctgaa caacctcgag ctgaactaag ttcaacttac cgttgtgaaa
     3661 gcaggtgacc gcccgccaag ggcggttgct tgctgtattg gacgaaccga ggaaaggggg
     3721 gtgcgggcag cctgtgcggg cgcatataga atgcgcccct acgaatgcgc tgccctgtag
     3781 gggccgggca tgcccggccc tctgcccggc ggtaatcgcc attcttcccc tataccacta
     3841 atatggtcgt cccactccca cgcaaggcgt cgtccctgcg tgggatgggg atgcccacat
     3901 tacggcattt ccctgcaaaa taaacgcggt ctaaccgtgc aaggaaggaa cgaaaactct
     3961 tgcgaaatta catcatcaag cgtatcgcgc agtcggtatt gatcctgttc tgtgtcatgt
     4021 tcattatcta cgcgctgatc cgcagcctgc ccagctcctt tgccgagacg atggcaatgc
     4081 agctggccca ggcccccggt gccaaaccct atgaggaatg gctggcccag ctcaatgcgg
     4141 cctacggcct tgacctggac attatccccg gcttcttcac ctggctgagc aaggccgtcg
     4201 tcggcaactt cggcgacagc tggaagtgga acgtgcccgc cacccagaag tttaccgagg
     4261 tcatcggcct gtctgtcatc atgggcggca tctcctttgt gctgtccatc gtgatcgccg
     4321 tgcccctcgg cgtgctggct gccaccaagc agtacagccg cacggactac gtcatcacgg
     4381 ctgcggcgct ggtcggtatt tcgctgctgt tctcggtcaa gctcggctgg ttcgatctgt
     4441 acggcctcgt cggccgtgac tatgctcagc tggacgcctg ggggcagttc tgcgataagg
     4501 ccaaccatct ggtgctgccc atcgcaacgc tggtcatcgt ctccatcggc ggctatatgc
     4561 gctacacccg cacgaatatg ctggaagtgc tgaacgcgga ctacatccgc accgcccgcg
     4621 ccaagggcct gtcggagcat acggtcatct acaagcacgc cttccgcaac acgctgattc
     4681 cccttgtcac catcatcggc ggcagcctgc ccggcctgtt cagcggtgcc cttatcaccg
     4741 agacgctgtt ctccatcccc ggcatcggct atatttccta ccagtccatg gtcgcaggcg
     4801 acatcccctt cacgatgttc tacctgtcct tcatggcggt gctgacgctg gccagcaacc
     4861 tgctgaccga tatcctgtac ggcgtggttg acccgcgcgt gcgcatttct taaagaaagg
     4921 agactgcaca cccatgagcg aaaacacgca gaaccagaac ccgaagcagg agcagtactc
     4981 cctgaacgat gaccgccgcg tcaaggtttt gtctcccggc gcactggtcg ccaagcggtt
     5041 cttccgcaac cgtctggctg tcgtcggtct gtccatcctg attgcaatgt tcctgttctc
     5101 cttcgtcggc ggccttgtca gcccctacgg ccaggacgag cagttcttca cctacaccca
     5161 gatgtccaag gagttcgtcg gcgtcacccg caacgacacg atgcgttttg tcgtggccga
     5221 cggccagaac ttcggttcca tcgcccagag caaggccctc gaagccgtga agaagggcag
     5281 caccgagttc aactataagg atactgacta caccgtcgat atccagagcg acgacttcta
     5341 tgtcgtctac cagggcagcg acgtcatggg ctatgccagc cgcgacctcg tcaacgaggc
     5401 cgacggtgcg ccgaagttca gctttgacgt caagctggcc gccctgaccg ccatgacggc
     5461 aggggagagc gacttcaccg ccgacggcgt ggactacacg ctgaacaagg acggcgagat
     5521 cgccgccaat ggcgagcagc tggggtatgt cagccgcttt gtcgtctccg ccgccgattc
     5581 cagcgtcgtc gttacccgcg acttcaagga ttgcctggaa gaggccatca acgagaacgc
     5641 cgataagttc aactacaccg acgccgaggg gaacgaggcc gagtacgata tcgtctacga
     5701 tgcctccacc aaagtttggt ccgtcaagca gatgaccgag acttacgtct acgaccgcta
     5761 cgccagcccc agcaaggcgc actggctggg caccgacacc aacggtatgg atatgctgac
     5821 ccgcctgatg tacggcggcc gcgtctcgct gatcatcggc ttcatcgtcg tcgtcatcga
     5881 ggcatccctg ggtattctga tgggcggtat ctccggctac ttcggcggct ggatcgacaa
     5941 catcattatg cgtgtcgtcg atgttttcta ctgcatccct tcgatgccgg tcatcatcat
     6001 catcggcgcg gccatggacg ccatgcgtgt ggattcctgg aaacgtatgc tgtacctgat
     6061 gctgattctc ggcttcctgg gct
//
LOCUS       DBMDSQ010000102         6048 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_3307_length_6048_cov_2.006726, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000102 DBMDSQ010000000
VERSION     DBMDSQ010000102.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 6048)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6048)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6048
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3307_length_6048_cov_2.006726"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..470)
                     /locus_tag="ACZPAX_05030"
     CDS             complement(<1..470)
                     /locus_tag="ACZPAX_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005923470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraslipin"
                     /protein_id="HIR1113295.1"
                     /translation="MLLIFAILLIIILVVLVRCIVIVPQSNAYVTEWLGVYKDTWGAG
                     LHIRTPFVERISRKVSLKEEAADFPPQPVITRDNVTMMIDTVVFFQVFDAKMYAYGVN
                     RPIQAIENLSATTLRDIIGSMTLDETLTSRDLINTKITASLDEATDRWGIKVNRV"
     gene            complement(498..947)
                     /locus_tag="ACZPAX_05035"
     CDS             complement(498..947)
                     /locus_tag="ACZPAX_05035"
                     /inference="COORDINATES: protein motif:HMM:NF014062.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HIR1113296.1"
                     /translation="MWFDTILWLVAIVAFIAVEAATTALVSVWFAVGAAAAMAASFFN
                     IGFGWQALVFAVVSAITLALMIPTLASRRGEHKAPVTNGSPLTIGKQGVVLVDIEPGT
                     LGRVRVDGLDWQARAETPLPKGERCKVVAVDGAILIVVPVTVNAVAE"
     gene            complement(1056..1499)
                     /locus_tag="ACZPAX_05040"
     CDS             complement(1056..1499)
                     /locus_tag="ACZPAX_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006574878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-thiouracil desulfurase family protein"
                     /protein_id="HIR1113297.1"
                     /translation="MKILVSACLLGVACKYSGGDNVCPALLNAVQHSGHTFIPICPEV
                     YGGLPTPRPPAERTGDCVLTETGTDVTAQYQRGAEAALQLARLTGCEAAILKANSPSC
                     GCGTIYDGTFRHRKIAGDGVTAELLQAHGLCVYNEDNFSELLRYV"
     gene            1713..2870
                     /locus_tag="ACZPAX_05045"
     CDS             1713..2870
                     /locus_tag="ACZPAX_05045"
                     /inference="COORDINATES: protein motif:HMM:NF013740.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HIR1113298.1"
                     /translation="MKKYFDRPPSTMGDWLVLAAGTALLFAAVARLPAALMALGGLLA
                     LLAPFGWGLVLAYVLDIPTRFFAQKLFGGRRGWAMGASYALFFGVLALLAALVVPQLV
                     QSITAFAGRLAAYEKTIRQLLLWVQATFGIDMATAEQLVQTVGSELQGWFASLSRQAA
                     QAAARAAGDAAGAAANAFVALAVSIYLLSGKENLLHAAHTCLRAALPPRAAGSVLAVC
                     TLANRIFSGYLGGQLVDALLVGGETFALMSIFGLEYAPLLAVLVGVTNIVPVLGPFLG
                     AVPGIVILLLESPWKAAEFAIIILVVQQVDGNFIAPRILGGATGLPGLGVLLAIVVGG
                     AWFGIPGMALGVPTLAVVAALLKQAVGAGLAARGLDADALDRDFPPMDTQN"
     gene            2944..4122
                     /locus_tag="ACZPAX_05050"
     CDS             2944..4122
                     /locus_tag="ACZPAX_05050"
                     /inference="COORDINATES: protein motif:HMM:NF013740.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HIR1113299.1"
                     /translation="MKDEHIWSKKPETILDWFCVVGACIAFYLILNNLGYFLGKIGFF
                     IGILSPFAGGVVIAYILDPMVKFFYHKLFKGKNKTRGIAILMAYAVAILLIVLLAWLV
                     IPQIVNSIVMLFTNFPSYIQGVQDLLVMMQDRYGIDLQQAIKMLDDSETMVKEVYSLA
                     TAAMPQIVDSIGSVASNFVGVFTSVAASIYMLADKEHLLHQLRTLAHAFLPEKAADNT
                     LRICHYANVNFTGFFVGKIIDSAIIGVITFVAMTILRLDFALLISVFIGITNIIPVFG
                     PFIGAIPSIFILLLVDPIQAVIFGVLILVIQQLDGNFIGPKILGSSIGISALWILFSI
                     VVGGDLFGIVGMVVGVPVFATLYGLAQEFVHYTLDKRGIDAEGNPREQTAEDKENVFE
                     "
     gene            complement(4259..>6048)
                     /gene="mfd"
                     /locus_tag="ACZPAX_05055"
     CDS             complement(4259..>6048)
                     /gene="mfd"
                     /locus_tag="ACZPAX_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HIR1113300.1"
                     /translation="RLGGNDWAKTRKKVKAATELMAKELIELYARRKQAHGYAFPSDD
                     TWQGDFEQRFAYEETPDQLTCAADIKHDMEEPWPMDRLLCGDVGVGKTEVALRAAFKC
                     VMGGKQCAILAPTTILAWQHFNTALTRMESFPIRIGLLSRYRTPKEQKETLRGLKDGT
                     VDIVVGTHRLLSNDVKFHDLGLVIIDEEQRFGVKHKEKLKENFIGVDMLTLSATPIPR
                     TLNMALSGIRDMSTIEQPPFERQPIETYVLEYDEGIVSEAIRKELARGGQVYYLHNRV
                     DTIDECAARIGKLIPGARIGIAHGKMTEEQISSVWQQLLDNEIDVLVCTTLIETGVDV
                     RNCNTLIIENADRMGLSQLYQIRGRVGRSSRKAYAYFTFTRDKVLTEIAAKRLSAIRE
                     FTSFGSGFRIAMRDLQIRGAGSLLGHSQHGHMEAVGYDLYVKMLGQAIATARGEAPAP
                     DKSDCLVDITVDAFLPEDYIPDAAGRIEAYKRIAAIETTTDAEDVLDELIDRYGSPPK
                     SVQGLVDVSLIRVTAARVGIAEIVQRGDQLILYSDIVGPRQLEQVMAQYPHRVLYNAL
                     GRPYFSLRVQKGENPLVLLRDVVTLLPGA"
BASE COUNT         1156 a         1748 c         1853 g         1291 t
ORIGIN      
        1 acgcggttga ccttgatgcc ccagcggtcg gtggcttcat ctaggctggc ggtgatcttt
       61 gtgttgatga ggtcgcggct ggtcagcgtc tcgtccagcg tcatcgagcc gatgatgtca
      121 cgcagagtcg tggcggacag gttctcgatg gcctggatcg ggcggttgac gccgtaggcg
      181 tacatcttgg cgtcaaacac ctggaagaaa acgacggtgt cgatcatcat ggtgacgttg
      241 tcgcgggtga tgacgggctg cggggggaag tcggcggctt cctccttcag ggaaactttg
      301 cgggagatgc gctcaacaaa cggcgtgcgg atgtgcaggc ccgcgcccca ggtgtcctta
      361 tagacgccga gccactcggt gacgtaggcg ttggactggg gaacaatgac gatgcagcgc
      421 accaaaacta ccagaataat aatcagcaga atggcaaaaa tcaacagcat ggggattcct
      481 cctttttctt ttggaactta ctctgcgacc gcgttgacgg tcacagggac tacgatgaga
      541 atggcaccgt ccacggccac gaccttgcag cgctcgcctt ttggcagcgg cgtttcggcg
      601 cgggcctgcc aatccaggcc gtccacccgc acgcggccga gggtgcccgg ctcaatatcc
      661 accagcacaa cgccctgttt gccgatggtc agcggggaac cgttggtgac aggggctttg
      721 tgctcacctc ggcggctggc aagggtggga atcatcagcg ccagcgtaat ggccgatacg
      781 acggcaaaaa caagcgcctg ccagccgaag ccaatgttga aaaagctcgc tgccatggcc
      841 gctgccgcgc ccaccgcgaa ccacaccgac acaagggcag tggtggcggc ttccacggcg
      901 ataaaagcaa caatggcgac aagccagagg atcgtatcaa accacatggc ggtactcctt
      961 tcggcaggga aaatgtattt atcactactt taacattctg ccgctgcgct gtcaagaatt
     1021 agttgtgggg taaaaatgta aatggttgcg caagactata cataacgcaa taattcgctg
     1081 aaattatcct cgttgtaaac gcaaagcccg tgcgcttgca gcaattccgc cgtcacccca
     1141 tccccggcaa ttttgcggtg ccggaatgtg ccgtcgtaga tcgtgccgca gccgcagctg
     1201 gggctgttgg cttttaagat ggccgcctcg cacccggtca gccgggccag ctgcaaggcg
     1261 gcctcggcac cgcgctgata ttgcgcagtc acgtcagtgc cggtttcggt cagcacgcag
     1321 tccccggtgc gctcggccgg cgggcgcggc gtgggcaggc cgccatagac ctcggggcag
     1381 atggggataa aggtatggcc gctgtgctgc acggcattca gcagggcggg gcagacattg
     1441 tcgccgccgg aatatttaca ggcgacgccc agcagacagg cgctgacgag gattttcatg
     1501 aaaaccaccg actttccgta aattgtgcgg tacaaccagt gtagcataaa aggaaagaaa
     1561 ccacaatgaa aaaatatttt gaccgcccac ccatggacac atataattaa caggttagct
     1621 cttgcaatgt acgcccacag cgcttacaat actactaatt ttccgtaaat tgtgcggtaa
     1681 acccagtgca gcataaaaga ggaggcacca caatgaaaaa gtattttgac cgcccgccga
     1741 gcacgatggg ggattggctg gtgctggcgg cggggacggc gctgctgttt gccgctgtgg
     1801 cacggctgcc cgctgccctg atggcgctgg gcggtctgct ggcgctgctg gcacccttcg
     1861 gctgggggct ggtgctggcc tacgtgctcg acattcctac gcgttttttt gcacaaaaac
     1921 tgtttggcgg gcggcgcggc tgggcgatgg gggccagcta cgcgctgttc ttcggcgtgc
     1981 tggcattgct ggcggcgttg gtcgtgcccc agctggtgca aagcatcacc gcctttgccg
     2041 gacggttggc cgcctatgag aaaaccatcc gccaactctt gctctgggtg caggcgacct
     2101 ttggcatcga catggccacg gcggaacagc tggtgcagac cgtggggagt gagctgcaag
     2161 gctggttcgc gtcgctctcc cgccaggcgg cccaggcggc ggcccgcgcg gcgggggatg
     2221 ctgcaggcgc cgccgccaat gcctttgtgg cgctggcggt cagtatctac ctgctctccg
     2281 gcaaagaaaa tctgctgcac gccgcgcaca cctgcctgcg ggcagcgctg ccgccccggg
     2341 cggcgggcag tgtgctggct gtatgcacac tggcaaaccg gatcttcagc ggctacctgg
     2401 gcgggcagct tgtggatgcc ctgctggtgg gtggggaaac cttcgccctc atgtcgattt
     2461 ttgggctgga gtacgccccg ttgctggcag tgctggtggg cgtgacgaac attgtgccgg
     2521 tgctggggcc gttcctcggc gcggtaccgg gcatcgtcat cctgctgctg gaatcgccct
     2581 ggaaagcggc agaatttgcc atcatcattt tggtagtgca gcaggtggac ggcaacttca
     2641 ttgcgccgcg catcctgggc ggggccaccg gcctgccggg gctgggtgtg ctgctggcca
     2701 ttgtggtggg cggtgcttgg tttggcatcc cgggcatggc gctgggcgtg cccacgctgg
     2761 ctgtggtagc ggcgctgctc aagcaggcgg tgggggccgg gctggcagcc cgcggcctgg
     2821 acgcagatgc cctcgaccgg gatttcccgc ccatggacac acaaaattaa caggttagct
     2881 cttgcaatgt acgcccgcag cgcttacaat actactaata tggtataaaa aggggcggag
     2941 cgcttgaaag acgaacatat ctggtccaaa aagccggaga cgatattgga ttggttctgc
     3001 gtggtcgggg cctgtatcgc attttatctg attttaaaca acctcgggta tttcctgggc
     3061 aaaatcggct tctttatcgg gattctgtcg ccgtttgcgg gcggcgtggt cattgcctac
     3121 atcctggatc cgatggtgaa attcttttac cacaagctgt tcaagggaaa aaacaagaca
     3181 cgcggcattg ctattctgat ggcctatgcc gtggcgatcc tgttgatcgt gctgctggca
     3241 tggctggtca tcccgcagat cgtgaacagt atcgtcatgc tgtttacgaa cttccccagc
     3301 tacatacagg gtgtgcagga tctactggtc atgatgcagg atcgctacgg catcgacttg
     3361 cagcaggcta tcaaaatgct ggacgattct gagaccatgg tcaaggaagt gtacagcctg
     3421 gcgaccgccg ccatgccgca gatcgtggac agcatcggca gcgtggcgtc caactttgtg
     3481 ggcgtcttta cttcggtggc ggccagtatc tatatgttgg cggataaaga gcatctgctc
     3541 caccagctgc gcacgctggc acacgccttt ttgccggaga aagcggctga caatacgctg
     3601 cgcatctgcc attacgccaa cgtgaacttt accgggttct tcgtcggcaa gatcatcgac
     3661 tcggccatca tcggcgtcat cacctttgtg gcaatgacga tcctgcggct ggactttgca
     3721 ctgctcatca gtgtgtttat cggcatcacg aacatcatcc cggtgttcgg ccccttcatc
     3781 ggcgcaattc ccagcatctt tatcctgctg ctggtcgacc ccatccaggc ggttattttc
     3841 ggtgtgctca tccttgtcat ccagcagctg gacggcaact tcatcggccc gaagatctta
     3901 ggctcgtcca tcggtatttc tgccctgtgg attttgttct ccatcgtggt gggcggcgat
     3961 ttgttcggca tcgtcggtat ggtcgtcggt gtgccggtgt tcgccacgct ctacggcctg
     4021 gcccaggagt ttgtgcatta cacgctggat aagcgaggca tcgacgctga gggcaaccca
     4081 agggaacaaa cggccgagga taaggaaaac gtgtttgagt aaaggggctt tgcccgcttg
     4141 ccgcaaaaaa taagccacgg tttacagaac gagcctgtaa accgtggcta tttctatttt
     4201 gtttaaaatt cttttcggtt ctttttcttt taggaaaagg aataaaaccc ccgcaaactc
     4261 atgcccccgg cagcagcgtg accacatccc gcagcagtac cagcgggttc tcgcccttct
     4321 ggacacgcag gctgaaatag gggcggccca gggcgttgta caggacccgg tgcgggtact
     4381 gcgccatgac ctgctccaac tggcgcggac cgacgatatc actatacaag atcagctggt
     4441 cgccgcgctg tacgatctcc gcaatgccaa cgcgggccgc cgtcacgcgg atgagagata
     4501 catccaccaa cccctgcacg ctcttgggcg ggctgccgta acggtcgatg agttcgtcca
     4561 gcacatcctc tgcgtcggtg gtcgtctcga tggccgcaat gcgcttgtag gcctcgatgc
     4621 gcccggcggc gtccggaatg tagtcctccg gcaggaaggc gtccaccgtg atatccacaa
     4681 ggcagtcgct cttgtccggg gccggggcct cgccgcgggc cgtggcaatg gcctggccca
     4741 gcatcttcac gtacagatcg tagcccacgg cctccatatg gccgtgctgg ctgtggccca
     4801 aaaggctgcc cgcgccgcgt atctgtaaat cgcgcatagc gatgcggaaa cccgagccga
     4861 aggaggtgaa ctcccggatg gccgaaagcc gcttggcggc aatttctgtc agcactttat
     4921 cgcgcgtaaa cgtgaaatac gcatacgcct tgcgggagga acgtcccacg cggccgcgta
     4981 tctggtacag ctgcgacagg cccatccggt ctgcattctc gatgatcagc gtgttgcagt
     5041 tgcgcacatc gacgccggtc tcgatcagcg tcgtgcagac aagaacatcg atttcattgt
     5101 ccagtaattg ctgccacacc gacgaaatct gttcttccgt catcttgccg tgggcaatgc
     5161 cgatgcgcgc gcccggtatc agcttgccga tgcgggccgc gcactcgtcg atggtatcca
     5221 cacggttgtg caggtagtag acctgcccgc cgcgggccag ctccttgcga atggcttccg
     5281 agacgatgcc ctcgtcgtat tccagcacat aagtctcgat gggctgacgc tcgaacggcg
     5341 gctgttcaat cgtggacata tcgcggatgc cggacagcgc catgttcagc gtgcggggga
     5401 tcggcgtggc cgacagggtc agcatatcga caccgatgaa gttttctttg agcttctctt
     5461 tatgcttgac gccgaagcgc tgctcctcgt cgatgatgac aaggcccaga tcgtggaact
     5521 ttacatcgtt ggacagcagc cggtgcgtgc ccacaacgat atccaccgtg ccgtctttta
     5581 aaccgcgcag cgtctctttt tgctccttcg gcgtgcggta gcgggacaac agcccgatgc
     5641 ggatggggaa ggactccatc cgggtcagcg ccgtgttaaa gtgctgccac gccaaaattg
     5701 tggtgggggc caggatggcg cactgcttgc cgcccatcac gcacttgaac gccgcccgca
     5761 gcgcaacctc ggttttgccg acgccgacat cgccgcagag caggcggtcc atcggccacg
     5821 gctcctccat atcgtgcttg atgtccgccg cgcaggtcag ctggtcgggc gtttcctcgt
     5881 aggcgaagcg ctgttcaaaa tcgccctgcc aggtgtcgtc ggaggggaag gcgtagccgt
     5941 gggcctgctt gcggcgggcg tacagctcaa tcagctcttt cgccatcagc tccgtggcgg
     6001 ccttgacctt tttgcgggtc ttggcccagt cattgccgcc caggcggc
//
LOCUS       DBMDSQ010000103         5931 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_3372_length_5931_cov_1.439794, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000103 DBMDSQ010000000
VERSION     DBMDSQ010000103.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 5931)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5931)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5931
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3372_length_5931_cov_1.439794"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            122..595
                     /gene="nrdR"
                     /locus_tag="ACZPAX_05060"
     CDS             122..595
                     /gene="nrdR"
                     /locus_tag="ACZPAX_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HIR1113301.1"
                     /translation="MKCPYCGEVDSKVIDSRPTEDGEKIRRRRECLNCKKRFTTYEIV
                     ETVPLMVVKKDRSREVFDRQKLLNGMLRACEKRPVSYTQLDAAVDRIEQQLLNSYDRE
                     VSSIHIGDLAMQELKQMDDVAYVRFASVYRQFSDVNTFMEELKDMLDSRSKPAEK"
     gene            complement(575..943)
                     /locus_tag="ACZPAX_05065"
     CDS             complement(575..943)
                     /locus_tag="ACZPAX_05065"
                     /inference="COORDINATES: protein motif:HMM:NF025634.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4363 family protein"
                     /protein_id="HIR1113302.1"
                     /translation="MKRNWYAAAILLVLCLLLYLAGRYVTDTTDAMQAGVQRAYALAE
                     QGDYPAARAAYAQTTRRAERDSALLALLVRRNIIDQLNQTMAVLPPCAEADSLADLEM
                     ETQRACAQIAQVRQSFFGGF"
     gene            complement(953..1603)
                     /locus_tag="ACZPAX_05070"
     CDS             complement(953..1603)
                     /locus_tag="ACZPAX_05070"
                     /inference="COORDINATES: protein motif:HMM:NF016154.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HIR1113303.1"
                     /translation="MISSVVRTVLVYLAVVVAMRLMGKRQLGELQPSELVTTLLISNV
                     ASICIDEPDLPLSASLVPIFLITALEILNSTLVWFCPKYAQLLLGKPVTIIRNGEIQQ
                     NELAQLRITASDLAEALRGKDIFSPEDVYWGVVEPNGSITAAPMPQDGEAPPMLPLLI
                     DKAVYKENLAFFGMDAAALDALLVRQNVTREKVLMLLYNGEKIVLIQKKAAPKGTV"
     gene            1671..3116
                     /locus_tag="ACZPAX_05075"
     CDS             1671..3116
                     /locus_tag="ACZPAX_05075"
                     /inference="COORDINATES: protein motif:HMM:NF015292.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spore germination protein"
                     /protein_id="HIR1113304.1"
                     /translation="MPQKPLNRSLDANLAFLDEMFGHSDDFYTKRLMIAGVPCAAIMF
                     TGLSSPEKLCRMALDMLDRDPAMLGGGEGLCDYLLTQSRIPAEPDAITDETTLIEMLS
                     NGLSVLLIEGVAKAVAFSTQEMPQRSVSTPTGEGNLRGPQEAFTELLRNNISLLRRQF
                     RTGTLAAEIYTARTRAKTEYCLCYDSRLAPQETIDALRARLAAVEIPVLLDSAYFASF
                     LKQDKLNLFPAAAYTERPATACARLCEGKAVVLVAGCPYALIIPSFFAEHFECLDDYA
                     SSAVFAGLIRILKYLAFLLAVFGPGLYVMAVSFAPEIIPIQLLTKLAQGETSTPLPPM
                     MEMLCVTLLLEIVREAGLRAPQSISHTVSLVGALIIGETAVSAGIVSVPVLTMAAAAT
                     IATLAVPSLYEQTILFRFAVILLAGCFGVPGLACAALTILAMACGSEPFGYDYLYPLL
                     PPTHASLRDGFVRSIWSRLAQSGEVLSRDET"
     gene            3106..4104
                     /locus_tag="ACZPAX_05080"
     CDS             3106..4104
                     /locus_tag="ACZPAX_05080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113305.1"
                     /translation="MKRECPSLFAALLISLLVNVLLRSQTAYWPRAGLAAALVCVPIL
                     AAAGWLFARCWRLAADPVYKLLFVALLFYTSVLELLRLWQLAGRLYPGAVTLTSVCLL
                     CVLPVVYLRRVSALSQTAHAVLCLLVLVTAVMLVSVLPRLRVTNLQTAPLTQGNFRAA
                     AKDQLTLYPEYLLPALWPEQDKRGRHALLRLAGYTLAFDVGMHLLLELFYGAAMPLRV
                     DPVHAAARCGALSIFNRLEWLQFILWVMAVTVKLALYLYALVRLLGGQSKAENSAAGL
                     DRFPLYLGGIWLLCAVLRKLDLDAALTLRSTMTWGFVVLTWMGGAAACLCKKLRRA"
     gene            4074..4988
                     /locus_tag="ACZPAX_05085"
     CDS             4074..4988
                     /locus_tag="ACZPAX_05085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113306.1"
                     /translation="MSVQKVKACLILCVAVLLCGCGQALSEREIVRAVFFAQQQGQYS
                     VCLLLADQNGGEDGVHYKTASARGATPAQALHRTEDTLPGQLYYGLLDLAALPPGSDW
                     QTAQTLGTLLYDKAQPAPELSVFVLDDAAYSWAQDAANLYEDMKAVEQEYALHCGLQQ
                     LFTQADGCAVPGYRTDSGYDFYLLAKDAPARRVSGLPAQLAAVLCGQADNFFSAYHDG
                     QALCKSEVNVTVEDTAVQLHLRDCTLQSLADADDDLQGFLCAELQHAFAALTNDAKAD
                     FFHLQFWHENLYGVGREAPAPTLAVVFE"
     gene            complement(5079..>5931)
                     /locus_tag="ACZPAX_05090"
     CDS             complement(5079..>5931)
                     /locus_tag="ACZPAX_05090"
                     /inference="COORDINATES: protein motif:HMM:NF025644.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DHHW family protein"
                     /protein_id="HIR1113307.1"
                     /translation="MFTKLFTVDDATKKQLDKNITAVSDFAANHPGKVTFLLAPSASV
                     IYPEALPLVAPMVDENAMLDDIFAKVGQNADVIDLREPFTELKDEYLYFKTDHHWTPN
                     GAYHAYEQFCTLKGLTPFDRTAHEAVTVTDFQGTHYSATRLWNVQNDEITYYPLDSLM
                     TVYKITGEAQYEPDKTENIVNADKFATRDKYAAFLDGNNGYSTIEGSGTGSILVVKDS
                     YANSFIPYLTENYAKIGVVDFRNFKYGLDSTIEKEGYDEVLVLYNFQTFIADTNLIYI
                     SRPSTIQ"
BASE COUNT         1087 a         1770 c         1788 g         1286 t
ORIGIN      
        1 tcggtatggt cagcggtgtg tacagcacga tttgcattgc tacgccgctg tgggtcgact
       61 ggaagaacaa gaaaaaggcc cccaagaagg cttgagcttg tagaaaagga gggtacccga
      121 tatgaaatgc ccctactgcg gtgaagtgga ttctaaggtc atcgattccc gcccgacgga
      181 ggatggcgaa aagattcgcc gccgccgtga gtgcctgaac tgcaaaaagc gctttaccac
      241 ctacgagatc gtcgagacag tcccgctgat ggtcgtgaag aaagaccgtt cccgcgaggt
      301 ctttgaccgc cagaagctgc tgaatggtat gctgcgcgcc tgcgagaagc gccctgtcag
      361 ctacacccag ctggacgctg ccgtagaccg cattgagcag cagctgctca actcttacga
      421 ccgcgaggtt tcctccatcc atatcggcga cctggctatg caggagctca agcagatgga
      481 cgatgtcgcc tacgtgcgct ttgcctctgt ctaccgccag ttcagcgacg tcaacacctt
      541 catggaagaa ctgaaggata tgctggacag ccgctcaaaa cccgccgaaa aatgattgcc
      601 gcacctgggc aatctgggca caggcgcgct gcgtctccat ttccaggtcg gccagactgt
      661 ccgcttcggc acagggcggc agcaccgcca tcgtctggtt cagctggtcg ataatgttgc
      721 gccgcaccag cagcgccagc aaggcgctgt cgcgctcggc ccgccgggtc gtctgcgcat
      781 aggctgcgcg ggcggcaggg tagtcgccct gttccgccaa ggcataagcc cgctgcacac
      841 ccgcctgcat ggcatcggtg gtgtcggtca cgtatcgccc cgccaggtac agcagcaggc
      901 agagcaccag caaaattgcg gctgcatacc agttccgttt cataagcacc tttcagaccg
      961 tccctttggg ggcggccttt ttctgtatca atacgatctt ttcaccgttg taaagcagca
     1021 tgagcacctt ttcccgggtt acgttctgtc ggaccagcag agcgtccagt gcggcagcat
     1081 ccatgccgaa aaaagcgaga ttttctttgt aaaccgcctt atcgatgagc agtggcagca
     1141 tgggcggggc ctcgccgtcc tgtggcatgg gcgcggccgt gatgctgccg ttcggctcca
     1201 ccacccccca gtagacatcc tcgggcgaga aaatatcctt gccgcgcagt gcctcggcca
     1261 aatcggacgc cgtgatgcga agctgggcca attcattttg ctgtatctcg ccgttgcgga
     1321 tgatcgttac cggtttgccc aagaggagct gggcatattt cgggcagaac cagacaagtg
     1381 ttgagtttag aatttccagc gcagttatta aaaaaatcgg cacgagactc gccgacagcg
     1441 gcaggtcagg ctcatcgatg cagatggacg cgacgttgga gatcagcagc gtcgtcacca
     1501 gctcggaggg ctgcaattcg cccagctggc gcttgcccat cagccgcata gccaccacca
     1561 cggccaggta caccagcact gtacgcacga ccgaagaaat cattcgggcc acctcctcat
     1621 aaatcccggc gaaagcggca acactatttt gcgtgagagg gggaacaccg atgccgcaaa
     1681 aaccgctgaa ccgttcgctg gatgcaaacc ttgcgtttct cgatgaaatg tttggtcatt
     1741 ctgatgattt ctatacaaag cgcctgatga ttgcgggcgt accctgcgcc gccattatgt
     1801 tcaccgggct atcctcaccg gaaaagctct gccgtatggc gctggatatg ctggaccgcg
     1861 atccggccat gctgggcggc ggggaggggc tgtgcgacta cttgctgacc caaagccgca
     1921 tcccagctga gccggacgcc atcaccgacg agacgacact tatagaaatg ctctccaacg
     1981 gcctgtccgt gctgctgatt gaaggtgttg ccaaggccgt ggcgttttcg acccaggaaa
     2041 tgccgcagcg gtctgtctcg acgcccaccg gcgagggcaa cctgcgcggg ccgcaggaag
     2101 cgttcaccga gcttttgcgc aataacattt cgctgctgcg gcggcagttc cgcaccggga
     2161 cactggcggc agaaatttac acagcccgca cccgcgccaa gaccgaatac tgcctgtgct
     2221 atgacagccg cctagccccg caggagacca ttgacgcact gcgggcgcgg ctggccgcgg
     2281 tagaaatacc cgttcttttg gatagcgcct actttgcgtc gttcctcaag caggacaagc
     2341 tcaatctgtt cccggctgcc gcctacaccg agcgcccggc cacggcctgc gcccgcctgt
     2401 gcgagggcaa ggccgttgtg ctggtggctg gctgccccta cgcgctcatc atccccagct
     2461 tttttgccga gcatttcgag tgcctggacg actacgcctc cagtgccgtg tttgcgggac
     2521 tgatccgcat tttaaaatat ctggcatttc tgttggccgt attcgggccg ggtctgtacg
     2581 tcatggccgt gtcctttgca ccggagatca tccccatcca gctgttgacc aagctcgccc
     2641 agggggagac atcgacgcca ctgccgccga tgatggaaat gctctgcgtg acgctgttat
     2701 tggagatcgt gcgcgaagcc gggctgcgcg caccgcagtc catcagtcac accgtcagcc
     2761 tcgtgggggc gctcatcatc ggcgagaccg ccgtcagtgc gggtatcgtc agcgtaccag
     2821 ttttaacgat ggccgcggct gctaccattg ccacgctggc tgtgccgtcg ctgtacgagc
     2881 agacgatttt gttccgcttt gctgttattc tgctggccgg gtgcttcggc gtgccggggc
     2941 tggcctgcgc cgcgctgacg attttagcca tggcctgcgg cagtgagcca ttcgggtatg
     3001 actacctcta cccgctgctg ccgcccaccc acgccagcct gcgggacggc tttgtgcgca
     3061 gcatctggag ccgcttggcc cagtccgggg aggtgctcag ccgcgatgaa acgtgaatgc
     3121 ccaagcctgt ttgcggcact gctgatcagc ctgctggtca acgtgctgct gcgcagccag
     3181 acagcctact ggccccgggc cgggctggcg gcggcgctgg tctgcgtgcc aatactggcc
     3241 gccgccggct ggctgttcgc ccgatgctgg cggctggccg ccgacccggt gtacaaattg
     3301 ctgttcgtgg cgctgctctt ctatacctcg gtactagagc ttctgcgcct gtggcagctg
     3361 gcggggcggc tgtaccccgg tgcggtcacg ctgacatcgg tctgcctgct ctgtgtgctg
     3421 ccggtcgtct atctgcggcg ggtgtcggcg ctgtcccaga cagcccacgc cgttttgtgc
     3481 ctgttggtcc tcgtcaccgc cgtgatgctg gtctccgtac tgccgcgcct gcgggtgacg
     3541 aatttgcaga ctgcaccact gacgcagggg aattttcgcg ctgccgcaaa agaccagctg
     3601 acgctctacc cggagtatct gctgcctgcc ctctggcccg agcaggacaa gcgtggccgc
     3661 cacgcgctgc tgcggctggc cgggtataca ctggcctttg acgtggggat gcacctgctt
     3721 ttggagctgt tctacggcgc ggccatgccg ctgcgggtcg accccgtaca cgccgccgcg
     3781 cggtgcgggg cgctttcgat tttcaatcgg ctggagtggc tgcaatttat tttgtgggtc
     3841 atggcggtca cggtcaagct ggcgctgtat ctctatgcgc tggtgcggct gctgggcggg
     3901 cagagcaagg ccgagaacag cgccgccgga ctggaccggt tcccgctgta ccttggcggt
     3961 atctggctgc tctgcgccgt gctgcgcaag cttgacctgg acgccgcgct gacgctgcgc
     4021 agcactatga cctggggctt tgtcgttctg acctggatgg gaggtgccgc cgcgtgtctg
     4081 tgcaaaaagt taaggcgtgc ctgattttgt gtgttgccgt gctgctctgt ggctgcggac
     4141 aggcgctttc tgaacgggag atcgtccgcg ctgtgttttt tgcgcagcag caggggcagt
     4201 attcggtctg cctgctgctg gccgaccaga acggcgggga ggacggcgta cactacaaga
     4261 ccgccagcgc ccggggtgca acgcctgccc aggccctaca ccggactgag gacacgctgc
     4321 ccgggcagct ctattacggc ctgctggatc tggccgccct gccaccgggc tcagactggc
     4381 agacagcgca gaccctgggc acactattgt atgacaaagc ccagccagca ccggagctgt
     4441 ctgtatttgt gctggacgat gctgcctaca gctgggcgca ggacgccgcg aatctatatg
     4501 aggacatgaa ggccgtggag caggaatacg ccctgcactg cggcttgcag cagcttttta
     4561 cccaggccga cggctgtgct gtgcccggct accgcaccga cagtgggtat gatttttacc
     4621 tgctggcaaa ggatgccccc gccaggcgcg tcagtggtct gcctgcccaa ctggcggcgg
     4681 tgctctgcgg gcaggcagat aatttttttt cggcgtacca cgatgggcag gcgctttgca
     4741 aaagcgaagt caatgtgact gtggaggata ccgccgtgca gctgcatctg cgggattgta
     4801 cgctgcaatc cctggccgat gcggatgacg atttgcaggg gtttctctgc gcagagttgc
     4861 agcacgcttt cgccgcgctt accaacgatg ccaaagctga ttttttccat ttgcaattct
     4921 ggcatgagaa tttgtatggc gtgggaaggg aagctccggc acccacattg gccgttgttt
     4981 ttgagtagag gcggcgtcct cgacgcactg ccatcgcaca tccatacaaa aaacgagcct
     5041 gaacgtttca cgaaacgttc aggctcgcct tcgttctttt actgtatcgt cgacggccgc
     5101 gaaatataga tcaaattcgt gtctgcgatg aaggtctgga agttgtaaag caccagcact
     5161 tcgtcatagc cctctttctc gatcgtcgag tcgaggccgt acttgaaatt gcggaaatcc
     5221 accacgccga tcttggcata attttcggtc aggtagggga taaagctgtt ggcgtagctg
     5281 tccttcacca ccagaatact gccggtgccg ctgccctcaa tcgtcgaata gccgttgttg
     5341 ccgtccagga acgccgcata cttgtcgcgg gtggcaaatt tatcggcatt cacaatgttc
     5401 tcggtcttgt ccggctcgta ctgggcctcg ccagtgattt tgtaaaccgt catcaggctg
     5461 tccagcgggt agtaggtgat ctcgtcattc tgcacattcc acagtcgcgt ggccgagtag
     5521 tgcgtgccct ggaaatctgt caccgtcaca gcctcatgtg cggtgcggtc aaagggcgtc
     5581 aggcccttca gcgtacagaa ctgctcatag gcgtggtacg caccgttggg cgtccagtgg
     5641 tggtcggtct tgaaatacag gtactcgtcc tttaattcag tgaacggttc ccgcagatca
     5701 atgacatcgg cattctggcc gacttttgca aagatgtcat cgagcattgc gttttcatct
     5761 accatcggtg cgaccagggg gagcgcttcg gggtagatga cactggccga cggtgccagc
     5821 aggaaggtga ctttgcccgg gtgatttgcg gcaaaatcgc tgactgccgt gatatttttg
     5881 tccagctgct tcttcgtggc atcgtccacg gtgaacagct ttgtaaacat c
//
LOCUS       DBMDSQ010000104         5916 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_3387_length_5916_cov_1.606019, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000104 DBMDSQ010000000
VERSION     DBMDSQ010000104.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 5916)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5916)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5916
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3387_length_5916_cov_1.606019"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..233
                     /locus_tag="ACZPAX_05095"
     CDS             <1..233
                     /locus_tag="ACZPAX_05095"
                     /inference="COORDINATES: protein motif:HMM:NF016362.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HIR1113308.1"
                     /translation="RFFGAMMGPPRVEKGLFITTAKFSKGAKEYADAQHIILVDGKQL
                     TSLMIEYGLGVSVQKVYQIKRIDSDYFADTLG"
     gene            256..1281
                     /locus_tag="ACZPAX_05100"
     CDS             256..1281
                     /locus_tag="ACZPAX_05100"
                     /inference="COORDINATES: protein motif:HMM:NF013884.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HIR1113309.1"
                     /translation="MNQEISRRISFLGFVMTCGIVLYHCGPSPAVPSVGLDQVCFDAI
                     SNFFTNTATLAMSYFFAVTGYLLFLNLSLRGWAGKVKRRTFSLLVPYLAWQCLTFAVN
                     LLHHEAMTKREFLKTTFLLDQWPPDGALWYLYAVFLLALLAPVILLIYERAGRCRGFC
                     LTFAAILFFYWLQSADFASAFCQYGYVNNILRYMPSYLLGAYFGFYCTQASEIDNLRF
                     LLAALLAALAADAVFSGFFSLMTLQVLPLFALFLLPIPEFLCDRKVYHLSFLVYALHQ
                     PLLKHLLHPIRDLMYVVYLASPAALINGLGRVLYLLAILCLARALHTTLSRFCPKALD
                     VLTGGRK"
     gene            1388..1780
                     /locus_tag="ACZPAX_05105"
     CDS             1388..1780
                     /locus_tag="ACZPAX_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HIR1113310.1"
                     /translation="MKGWFQRFMSGRYGFDQFGGFLCIASLILIVIGAWVSPILYWLG
                     LAAIIYSYFRILSRNTRKRYAENLKYLSYQNRVTTWLGKYQVRFKQRKDYHYYRCPKC
                     GQQLRVPRGRGKISITCPKCGNQFIKKS"
     gene            1787..2641
                     /locus_tag="ACZPAX_05110"
     CDS             1787..2641
                     /locus_tag="ACZPAX_05110"
                     /inference="COORDINATES: protein motif:HMM:NF039826.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5685 family protein"
                     /protein_id="HIR1113311.1"
                     /translation="MFGYVTIYRKGLADADMDRYQAYYCGLCRALGRRYGRTGQLALS
                     YDMAFVAILLTALYDVPTAYTAGRCAPHPIKPRPRADNEFMDYAADMTAALAYYNFMD
                     DWQDDHRRASLAQAQKLEPQLPALRERWPRQMQTMTDALDKLNALESAGSHDLDALCN
                     AFGALLGDVFACRDDIWAPALRAMGQGLGGFIYLMDAYDDLEKDEKEGRFNALRQLAD
                     ELPPAAYEQRCHELLTQQMGRCAQPFEMLPILKDTPEGKLLYNTIYSGVWSKYALVRK
                     HREGRRHD"
     gene            2634..3371
                     /locus_tag="ACZPAX_05115"
     CDS             2634..3371
                     /locus_tag="ACZPAX_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005921059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HIR1113312.1"
                     /translation="MTDPYSVLGITPGADDETIKKAYRQKCKQYHPDLHPDDPSAEEH
                     FKEVQAAYSEIMRIKQGGGATYRQSSQSYGQSGYGQQYQDPFSGGGFGFGPFGFGYYS
                     TSGSTGGQSYGSTAGDPELRAAANYIRSGFYEEAMNTLNGIPTANRTAQWHYYAALAN
                     QGLGNNIRAQEEARTAVSMEPNNYAYQNLLDQLQSPGRSYTSYQQPYAQPSGRPGSFC
                     LSFWLYLLVCNVLSWCCCGGRGGFYIC"
     gene            complement(3510..4193)
                     /locus_tag="ACZPAX_05120"
     CDS             complement(3510..4193)
                     /locus_tag="ACZPAX_05120"
                     /inference="COORDINATES: protein motif:HMM:NF015639.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HIR1113313.1"
                     /translation="MTAVNVEPGRCGENWDSCPHLTTGETFDFACAGCGDCCRKRRDL
                     VLSGYDLYRIARRLRLSPRIVASAFCKSYVADTTCLPALRLTPDPKTGDCRFFEGGAC
                     AIHAARPLACALYPLGQSIDTVTARMEYYVQTPLCGARAVKRTLQDYLNDAAIPDRAG
                     IDARWAIVCTQLSHKLQAAGGQNNPHFSAAAKRTERALYYDYSLGDEFYPQFCQNIEI
                     LMPLLDRML"
     gene            complement(4190..5176)
                     /locus_tag="ACZPAX_05125"
     CDS             complement(4190..5176)
                     /locus_tag="ACZPAX_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-isomer specific 2-hydroxyacid dehydrogenase
                     family protein"
                     /protein_id="HIR1113314.1"
                     /translation="MKIMFYTLRPFDEQQYCEPYKQQYGIDYTGTPGVPTPENLHLAE
                     GCDAVSTNPCAIRPEYLEAWAGMGVKYLPCRSIGYDHIPLDTAKKLGMRISHSHYPPE
                     GVANYTIMLILMCTRKMNQIMLRANVQDYSLPGKMGRDISDCTVGVIGTGKIGQTVVR
                     NLSGFGCKMLAYDLYPNDAVKKYADYVTLDELYARSDVITLHTNATAENHHLINADAI
                     AKMKDGVLLVNTARGTLIDPDALVTGLECGKIGGAGLDVIEDETGICYYNRMGEAIPN
                     RELNLLRSYPNVILSPHTAFYTDVNVANMVKSPFEALDAYAHGRPYAGEVQL"
     gene            complement(5192..>5916)
                     /locus_tag="ACZPAX_05130"
     CDS             complement(5192..>5916)
                     /locus_tag="ACZPAX_05130"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR1113315.1"
                     /translation="IRPAILILPGGGYAWCSSREADPVAMQFLQAGYNVFILTYTCRK
                     DDTQPPLRWQPLIDAAGAILHIRRNAAKFRVDPFKVAVCGFSAGGHLAASTAILWDAE
                     PVQKALGIHAEEARPDAVVLGYPVITSGIMTHGGSIKNLCGDDAELKATMSLENQIRG
                     DLPPFFIWHTVEDGSVPVKNSLLLASALTEHKVPFELHLFNHDGHGTSTCTREVNTPN
                     AHNRAWVDLCTDWLADLFNYHL"
BASE COUNT         1152 a         1674 c         1748 g         1342 t
ORIGIN      
        1 aaaggttctt tggtgctatg atggggccac ctagggtaga gaagggacta tttatcacaa
       61 cagcaaagtt ttccaaaggt gccaaggaat acgccgatgc acagcatatc attctggtag
      121 atggaaaaca gctcacttcc ttgatgattg aatatggtct tggcgtttcc gtacagaagg
      181 tttaccagat aaagcgcatt gacagtgact attttgcgga tacattaggc tgagaaaaag
      241 gagttttttc ttaccatgaa ccaggaaatc agcaggcgca tatcctttct ggggtttgtg
      301 atgacctgcg gcatcgttct ctatcactgc ggcccttccc cggctgtccc gtctgttggg
      361 ttggaccagg tatgttttga tgccatatca aacttcttca caaataccgc gacacttgcc
      421 atgtcctatt tttttgcagt gactggctat ttgctgtttc tgaatttatc gctgcgcggc
      481 tgggccggta aggtcaagcg ccgcactttt tcgctgttgg ttccctatct ggcgtggcaa
      541 tgtttgactt ttgcggtcaa tctgctgcac catgaggcca tgaccaaacg tgaatttctg
      601 aaaacgacct ttcttcttga ccaatggccg ccggatggcg cactgtggta tctctatgcg
      661 gtctttctgc tggcattgct tgcccctgtt attctgctta tttacgagcg tgcaggtcgt
      721 tgtcgtggct tttgccttac gttcgcggca attttgtttt tctactggtt gcaatccgca
      781 gattttgcct cggctttctg ccaatatggc tatgtcaata atattttgcg gtatatgccg
      841 tcctatcttt tgggcgctta cttcggattt tattgcacac aggcatcgga gatagataat
      901 ctacgctttc tgttggctgc gcttctggct gcacttgccg ccgacgccgt gttttctggc
      961 ttcttctcgc tcatgacgct gcaagtgctt ccgctgttcg cgctgttctt attgcctatt
     1021 ccggagttcc tgtgtgacag aaaagtctat catttgtcgt tccttgtgta cgccctgcat
     1081 cagccattgc tcaagcacct cttgcatccg atccgcgatt tgatgtacgt ggtatacctg
     1141 gcttcgcctg ccgcactcat caatgggctg ggacgtgtgt tgtatctgct tgctattttg
     1201 tgcctggccc gggcgctgca taccacgctt tctcggttct gcccaaaagc gctggatgtg
     1261 ctgaccggcg gacggaagta gtatttgttc aaactctatt tcccgcttgt tcacgatttg
     1321 cccatattta tatgtcatac taaagtgtaa acaatgcggc tttgccgcaa aaaggtggtt
     1381 tttataaatg aaaggctggt ttcagcgctt tatgtccggc cgctatgggt ttgaccagtt
     1441 cggcgggttt ctgtgcattg cctctctcat ccttattgtg attggggcgt gggtgtcgcc
     1501 gattctctac tggctggggc tggcggccat catttacagc tacttccgca ttctcagccg
     1561 caacacccgc aagcgctatg ccgaaaatct caaatactta tcctaccaaa accgcgtaac
     1621 gacctggctg ggcaagtatc aggtgcggtt caagcagcgc aaggactacc actactaccg
     1681 ctgccccaag tgcggtcagc agctgcgcgt gccccgtggg cgcggcaaaa tcagcatcac
     1741 ctgccccaaa tgcggcaacc agttcatcaa aaagagttga cgcccaatgt ttggatatgt
     1801 tacgatttat cgcaaggggc tggcagacgc cgacatggat cgctaccagg cctattactg
     1861 tgggctttgc cgggcgctgg gccgccgtta tggccgcacc gggcagctgg cgctgagcta
     1921 tgacatggcg ttcgttgcca ttttattgac ggcgctgtac gacgtcccca ccgcctacac
     1981 ggcgggccgc tgcgcaccgc accccatcaa acctcggccc cgggctgaca atgagtttat
     2041 ggactacgcc gccgacatga ccgccgcgct ggcgtactat aactttatgg acgattggca
     2101 agatgaccac cgccgtgcca gcctggccca ggcgcaaaag ctggagccgc agctacccgc
     2161 cctgcgggaa cgctggcccc gccagatgca gacgatgacc gacgcactgg acaagctgaa
     2221 cgccctggag agcgcaggga gccacgacct ggacgcgctg tgcaatgcgt tcggtgcgct
     2281 gctgggcgat gtttttgcct gccgcgacga catctgggcc ccggcgctgc gggccatggg
     2341 gcagggcctg ggcggcttta tctatttgat ggacgcctat gatgatttgg aaaaagacga
     2401 aaaagaagga cgtttcaacg cactgcgtca gctggcagat gaactgccgc ctgccgccta
     2461 tgagcagcgc tgccacgagc tgctgaccca gcagatgggc cgctgtgcgc agccgtttga
     2521 aatgctgccg atattaaaag ataccccgga ggggaaacta ttgtacaaca cgatctattc
     2581 cggcgtgtgg agcaaatacg cgctggtacg taagcaccgg gaggggcgca gacatgactg
     2641 atccatacag cgtattgggc atcactcccg gtgccgatga cgaaacgatt aaaaaagcct
     2701 accgtcaaaa gtgcaagcaa taccaccccg acctgcaccc cgacgatcct tccgccgagg
     2761 agcactttaa ggaagtgcag gccgcataca gcgagatcat gcgcatcaag cagggcggcg
     2821 gcgcgacgta ccggcaaagc agccaatcct acggccagag cgggtacggc cagcagtacc
     2881 aggacccctt cagcgggggc gggttcggct ttgggccgtt cgggttcggg tactacagca
     2941 caagcggttc aaccgggggg cagagctacg gcagcaccgc aggcgacccc gagctgcgtg
     3001 cggcggcaaa ctacatccgc agcggcttct atgaggaagc catgaacacg ctgaacggca
     3061 tccccaccgc caaccgcacg gcgcagtggc actactacgc cgcgctggcc aaccaggggc
     3121 ttggcaacaa tatccgtgcc caggaggaag cccgcacggc ggtatcgatg gagccgaaca
     3181 actacgccta tcagaacttg ctggaccagc tgcaaagccc gggccgcagc tacacgagct
     3241 accagcagcc ctacgcccag cccagcggcc gcccgggatc gttttgtttg tcgttctggc
     3301 tgtatttgct ggtatgcaat gtgctgagct ggtgctgctg cggcggcaga ggcggatttt
     3361 atatttgctg attttggata gaaaaggcgc tgtggccggt gtggtcacag cgccttttgt
     3421 tattattcgg cagggccgca catatgcggc ccgcaggcgt ttatgggaaa gttgagggcc
     3481 tggtatgccc gattcctact aattttgatt tacagcatcc tatccagcag cggcatcaaa
     3541 atttctatgt tttggcaaaa ctgcgggtag aactcgtcgc caagggaata gtcgtagtag
     3601 agggcgcgct ccgtgcgctt ggcggcggca gagaagtgcg ggttgttctg cccacccgcg
     3661 gcctgcaatt tatgggacag ctgcgtgcag acgatggccc aacgcgcgtc gatacccgca
     3721 cgatccggga tggcggcatc gttcaggtag tcctgcaagg ttcgtttcac tgcccgcgca
     3781 ccgcacagcg gcgtttggac gtaatactcc atcctggccg tcacggtatc aatgctctgc
     3841 cccagcgggt agagcgcaca ggccagcggc cttgcggcat ggatggcgca ggcgccgcct
     3901 tcaaaaaagc ggcagtcccc ggtcttaggg tcgggcgtca gccgaagcgc aggcaggcag
     3961 gtcgtatcgg ccacatagct tttgcagaag gccgatgcca cgatgcgggg cgacagccgc
     4021 agccgccgcg caatgcggta gaggtcatac ccggacagca ccagatcccg ccgtttgcgg
     4081 caacaatcgc cgcagcctgc gcaggcaaaa tcaaacgtct cccctgttgt caggtgcggg
     4141 cagctatccc agttttcacc gcagcggccc ggctccacgt tgacagccgt cacagctgca
     4201 cctcgcctgc gtagggccga ccgtgggcgt aggcgtccag cgcctcaaag ggcgatttga
     4261 ccatgtttgc cacgttcaca tcggtgtaga acgccgtgtg cgggctgagg ataacattgg
     4321 ggtagctgcg cagcaggttc agctcgcgat tggggatggc ctcgcccatg cggttgtagt
     4381 agcagatgcc ggtctcgtcc tcgatgacgt ccagccccgc gccgccgatc ttgccgcatt
     4441 ccagcccggt caccagcgcg tccgggtcga tgagggtgcc gcgtgccgtg ttgaccaaca
     4501 gcacgccgtc cttcatcttg gcgatggcgt cggcgttgat gaggtggtgg ttctccgccg
     4561 tagcgttcgt gtgcagcgtg atgacgtcgc tgcgggcata cagctcgtcc agcgtgacat
     4621 agtctgcgta tttctttaca gcgtcgttcg ggtagaggtc gtaggccagc atcttgcagc
     4681 cgaaaccgct caaattgcga acgacggtct ggccgatctt gccggtgcca atgacgccca
     4741 ctgtgcagtc cgagatatcg cggcccatct tgccgggcag tgagtagtcc tgcacgttgg
     4801 cgcgcagcat gatttggttc atcttgcggg tacacatcaa aatcaacatg atggtatagt
     4861 ttgccacccc ctccggcggg tagtggctgt ggctgatgcg catacccaat tttttggcgg
     4921 tatccagcgg gatatggtca tacccgatgc tgcggcaggg caggtatttt acgcccatcc
     4981 cggcccaggc ttccaggtat tcgggccgaa tcgcgcaggg gttcgtgctg acggcgtcgc
     5041 agccctcggc caggtgcagg ttctccggcg tgggtacgcc gggcgtgccg gtatagtcga
     5101 tgccgtactg ttgtttgtac ggctcgcaat actgctgctc gtcaaagggg cgcaatgtgt
     5161 aaaacatgat cttcatgttt tgtcatcttc cttacaggtg atagttgaag aggtcggcca
     5221 gccagtccgt acacagatcc acccaggcgc ggttgtgggc gttgggggtg ttgacctccc
     5281 gcgtgcaggt gctggtgccg tggccgtcgt ggttgaagag atgcagctca aacggcacct
     5341 tatgctcggt cagggccgac gccagcagca gtgagttctt cacagggacc gagccatcct
     5401 ccaccgtgtg ccagataaag aagggcggca gatcgccgcg gatttgattt tccaggctca
     5461 tggtggcctt cagttcggca tcgtcgccgc aaagattctt gatggagccg ccgtgggtca
     5521 tgatgcccga cgtgataact gggtagccca gcacaacagc atcggggcgg gcttcctcgg
     5581 cgtggatgcc gagggctttc tgcaccggct cggcatccca caggatggcc gtggatgccg
     5641 ccagatgccc gcccgccgag aagccgcaga cggcaacctt gaaggggtca acacggaact
     5701 ttgctgcgtt gcggcggatg tgcaaaattg cccccgctgc gtcgatcagc ggctgccagc
     5761 gcagcggcgg ctgggtatcg tccttacggc aggtgtaggt caggataaag acgttatagc
     5821 ctgcctgcaa aaactgcatg gcgacggggt cagcctcgcg cgagctgcac caggcgtagc
     5881 cgccgcccgg cagaattagt atagccgggc ggatgt
//
LOCUS       DBMDSQ010000105         5893 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_3399_length_5893_cov_2.237051, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000105 DBMDSQ010000000
VERSION     DBMDSQ010000105.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 5893)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5893)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5893
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3399_length_5893_cov_2.237051"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1610)
                     /locus_tag="ACZPAX_05135"
     CDS             complement(<1..1610)
                     /locus_tag="ACZPAX_05135"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005807027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050151 - oleate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HIR1113316.1"
                     /translation="MYYSSGNYEAFATPKKPANVDGKSAYLVGSGLAALSAACYLVRD
                     AQMKGEHVHILEKDPIPGGACDGYKYQDIGYVMRGGREMDNHFECMWDLFRSIPSIED
                     ENHTVLDEYYWLNKADPNYSLCRATRNRGEDAHTDGKFGLSDKAAMEIMNLFMSPDEK
                     LYDKKITDFFDDEVLNSNFWMYWRTMFAFENWHSALEMKRYLQRYIHHIGGLPDFTAL
                     RFTRYNQYESMILPMVKYLEGFGVQFHYDTKVTNIAFDCAGGKKQATRIDILHDGQEE
                     SIDLTENDLVFITNGGCVENSTLGSQDTPAEFKPELKEGGGWDLWRKIAAQDPSFGHP
                     DKFCYDPELSNWMSATITTLDQRIVPYIKKICKRDPFSGKVVTGGIVTVTDSNWLLSW
                     TLNRQQQFRDQPKDQLCVWVYGLFTDKPGDYIKKPMRDCTGKEICMEWLYHIGVPEDQ
                     IEDMATNSANTVPCMMPYIDAFFMPRAAGDRPDIVPEGAVNFAFLGQFAETARDTIFT
                     TEYSIRTGMEAVYTLLDVDRGVPEVWGSV"
     gene            complement(2101..2661)
                     /locus_tag="ACZPAX_05140"
     CDS             complement(2101..2661)
                     /locus_tag="ACZPAX_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014793420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR1113317.1"
                     /translation="MSQMTKRALVASLKDLLAEKPLDKITVTDLTEHCGVNRMTFYYH
                     FKDIYDLVEWACIESATRALAGQKTYDTWQQGFLQILQAVQKDKVFVTKVYHSISREH
                     IENYLYRLTYDLMFGVVEEKAAGMTVRPEDKEFIANFYKYAFVGLTLEWVRTGMKDDP
                     AALVERVSVLIHGDITKSLETCRIDK"
     gene            3029..3616
                     /locus_tag="ACZPAX_05145"
     CDS             3029..3616
                     /locus_tag="ACZPAX_05145"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002849446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR1113318.1"
                     /translation="MPEMLDIVDENGVPTGRSVPRTVAHAEGLRHRTSHVWIVRRKAG
                     RVQVLLQMRCAAKDSYPGCYDISSAGHIPAGDEFVGSALRELREELGVDARAEDLHEC
                     GLRRFRFEAEFHGKPFKDNQVSKVFCLWLDKEAEDFNVQKSEIDYVKWFDLDEAIRAV
                     DEGTIPNCIFPEELGMVKEFVQKASPLGEAGREAD"
     gene            4102..5340
                     /locus_tag="ACZPAX_05150"
     CDS             4102..5340
                     /locus_tag="ACZPAX_05150"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007048368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HIR1113319.1"
                     /translation="MKKDKKDIKKVVLAYSGGLDTSIIIPWLKENYNNCEVVAVSGDV
                     GQGTELDGLEEKAIKTGASKLYVLDLKKDYVENYIWPCLKADAKYEDYLLGTSHARPC
                     IAAALAEIAKKENADAICHGCTGKGNDQVRFELTLKALAPDMAIIAPWREWSIKSRDE
                     EIDYAEAHNIPLKINRETNYSKDKNLWHLSHEGLDLEKPSLEPQYNKPGFLELGVSPE
                     QAPDKPTYVKIHFEKGIPTAVDGKEMESVELVEYLNKLGGANGIGLLDIVENRLVGMK
                     SRGVYETPGGAILYKAHDVLETITLDKESMHFKAQLAQKMGELVYNGQWFTPLREAIS
                     AFTDDLQKTVTGDVTLKLYKGNMINAGVTSPYTLYDEQTASFGEDDDYNQADANGFIN
                     LFGLPIAERAKLAKNWPTQD"
     gene            5631..>5893
                     /locus_tag="ACZPAX_05155"
     CDS             5631..>5893
                     /locus_tag="ACZPAX_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007048369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113320.1"
                     /translation="MPQLWQGRASKAVDSRVNDFNSSIRFDARMIEQDIQGSLVHSAM
                     LGRQGIISQQDVDDIHKGLHSILEDLHSGALIGFLWYNAFPAQV"
BASE COUNT         1287 a         1620 c         1758 g         1228 t
ORIGIN      
        1 acgctgcccc agacctcggg cacgccgcgg tctacatcca gcagggtata gacagcctcc
       61 atgccggtgc ggatggagta ctcggtcgtg aagatcgtgt cgcgggcggt ctcggcaaac
      121 tggccgagga acgcaaagtt cacagcgccc tcgggtacga tgtcggggcg gtcgccggca
      181 gcgcgcggca taaagaacgc gtcaatatac ggcatcatgc agggcacggt gttggcgctg
      241 ttggtcgcca tatcctcgat ctgatcctcg ggcacaccga tgtggtacag ccactccatg
      301 cagatctcct tgccggtgca gtcgcgcatc ggcttcttga tatagtcgcc cggcttgtcg
      361 gtgaacaggc cgtagaccca gacgcagagc tgatccttgg gctggtcgcg gaactgctgc
      421 tggcggttca gcgtccagct gagcagccag ttggaatcgg tgaccgtgac gatgccgccg
      481 gtgacgacct tgccgctgaa cgggtcgcgc ttgcagattt ttttgatata gggcacgatg
      541 cgctgatcca gcgtcgtgat ggtggcgctc atccagttgg acagctcggg gtcatagcag
      601 aacttgtcgg gatggccgaa gctcgggtcc tgcgcggcga tcttgcgcca caggtcccag
      661 ccgccgccct ccttcagctc gggcttgaac tcggcggggg tatcctggct gccgagggtc
      721 gagttctcga cacagccgcc gttcgtgata aagacgaggt cgttctcggt caggtcgatg
      781 gactcttcct gcccatcgtg gaggatgtcg atgcgggtgg cctgcttctt gccgccggca
      841 cagtcgaagg cgatgttcgt gaccttggtg tcgtagtgga actgaacgcc gaagccctcc
      901 aggtacttga ccatcggcag aatcatcgac tcatactggt tgtagcgggt gaagcgcagc
      961 gccgtaaagt cgggcagacc gccgatgtgg tggatgtagc gctgcagata gcgcttcatc
     1021 tccagtgcgc tgtgccagtt ctcaaaggcg aacatcgtgc gccagtacat ccagaagttg
     1081 gagttcagga cctcatcgtc gaagaagtcg gtgatcttct tgtcgtacag cttttcatcg
     1141 ggggacatga acaggttcat gatctccatg gcggccttgt cgctcagacc gaacttgccg
     1201 tcggtgtggg catcctcgcc gcggttgcgg gtggcgcggc agaggctgta gttggggtca
     1261 gccttgttca gccagtagta ctcatccaga acggtgtggt tctcgtcctc gatggaggga
     1321 atggagcgga acagatccca catacactca aagtggttgt ccatctcacg gccgccgcgc
     1381 atgacatagc cgatgtcctg atatttgtag ccgtcgcagg cgccaccggg gatggggtcc
     1441 ttctcgagaa tatggacgtg ctcgcccttc atctgggcat cgcgcaccag atagcaggcg
     1501 gcagacagcg ccgccaggcc ggaaccgacg agataggccg atttgccgtc aacgttggcg
     1561 ggctttttgg gggtggcaaa ggcttcatag ttaccgctgg aataatacat aacgtacctc
     1621 ctgtaagtgt atacggcggg ggtgggtgca ggggccgatg ctcgtcatcg gcctgcccgc
     1681 gtgccgttgg ttgtttggta tggccttagt atacccggta ggcgcaccgg ttacaatcag
     1741 caaaaaatcc cctttgataa gaattttatc aaaggggagg gattgtatag attttgaaaa
     1801 aaatgttaaa acgcggcttt gtagggggcg gcgtcctcga cgccccgcgg aggggtcaac
     1861 gcttggcggc gcacaacgag cgccgccagt tcgcttcgcg aacccgctcg tgcggttgtt
     1921 tgccccgcag gggcaaacat tgctcgctcc ccagtttcgc ttcgctcaac cgtggccccg
     1981 cgctcgcaac cccgcaaccc ttccctgcaa atccattgaa aaatggggat gcggccataa
     2041 acttgcgggc cgggcatacc cggccccaac gaagcggcgc gtttgaggtg gcaatgaaaa
     2101 ctatttatcg attctgcacg tttccaggct cttggtgata tccccgtgga tcagcacaga
     2161 cacccgctcc accagggcgg cggggtcgtc cttcatgccg gtgcggaccc attccagcgt
     2221 caggccgacg aaggcgtact tgtaaaagtt cgcaatgaac tccttgtcct cggggcgcac
     2281 ggtcatcccg gcggcttttt cctcgacgac gccgaacatt aaatcgtagg tcaggcggta
     2341 caggtagttt tcgatgtgct cgcggctgat ggagtggtag actttcgtaa cgaacacttt
     2401 gtccttctgc acggcctgca agatctgcaa aaagccctgc tgccaggtgt cgtaggtttt
     2461 ctgcccggcc agcgcacggg tggcgctctc gatgcaggcc cactcgacca ggtcgtagat
     2521 atccttaaaa tggtagtaga aggtcatgcg gttgacgccg cagtgctcgg tcaggtcggt
     2581 gacggtgatc ttgtccagcg gcttctcggc cagcaggtct ttcagcgacg caacgagcgc
     2641 gcgtttggtc atttgggaca tgggagtctc cttttatata atgcggtacg gcggacgcgg
     2701 ggcgtcgggg acgccgcccc ctacggaagc tgctttacat tatataggaa gattatgaat
     2761 ttttaaaccc cgcagccttt ttatacaggg tggtgacccg ctcccggggg agaaggtagt
     2821 tgatgagcag tgccacaatg acgccgacgg cggtggattc tggttcatcg ggtactcctt
     2881 tgtttgttaa ataacagact aagtataaaa tttttcttga ctaaaacagc ataccatgaa
     2941 aatgcgggcg gcgcaagaaa aaattactga cgcagccgca aaaatgtggt ataacggtag
     3001 tattgaatta aaaaaggaag tgtaccccat gcctgaaatg ctggatattg ttgacgaaaa
     3061 cggcgtaccc accggccgca gcgtgccgcg caccgttgcc cacgccgagg ggctgcgcca
     3121 ccgcaccagc catgtgtgga tcgtgcggcg caaagcgggc cgcgtgcagg tgcttttgca
     3181 gatgcgctgt gcggccaagg attcctaccc agggtgctac gatatctcct cggccgggca
     3241 tatcccggcg ggggatgagt ttgtcggctc ggccctgcgg gaactgcggg aggaactggg
     3301 cgttgatgcc cgcgctgaag atttgcacga gtgcggcctg cggcggtttc gttttgaggc
     3361 ggagttccac ggcaagccgt ttaaggacaa ccaggtcagc aaggtgttct gtttgtggct
     3421 ggataaagag gccgaggatt tcaacgtgca gaagtcggag atcgactatg tgaagtggtt
     3481 cgatttggac gaggccatcc gcgccgtgga cgaggggacg ataccgaact gcatctttcc
     3541 ggaggagctg gggatggtga aagagtttgt ccaaaaggct tctcccctgg gagaagctgg
     3601 ccgcgaagcg gactgatgag gggcggcctt gcctggctgc gtctttgcgg gacggttgac
     3661 cgaacgcgcc cctcatccgg cctgcgggct gccttcagtc tacgcgctaa gagccgccta
     3721 cggcggttgc gctccgacac gtgcctgcgg gcgcagcccc gcaggggaag gcgtgggggc
     3781 ttttccgttg gcaccacccg tgttgcaaaa cgcgcaattt tgtgttgctt acccgggaaa
     3841 accatgctat aatacccata gcggcgctgt gtatacatac acgcctgtat atttttgcaa
     3901 gcatcccctg cataaatgta taactattcg gcaacctgcc taaaacaaag ccaaaagtag
     3961 aaaatccttt ggcaggtttg ccccttgcaa aatgtataaa agagtgtata atatacagca
     4021 atgaatgaat aaatattcaa gacacacagc aagcaacagc accctgcggg tcttggcaaa
     4081 tacggagagg ggaatttcat catgaaaaag gataagaaag atattaaaaa ggtagttctg
     4141 gcatactccg gcggtctgga cacctccatc atcattccct ggctgaagga aaactacaac
     4201 aactgcgaag tcgtcgctgt ctccggtgac gtcggccagg gcaccgagct ggacggcctg
     4261 gaagagaagg ccatcaagac cggcgcttcc aagctgtacg tcctggatct caagaaggac
     4321 tacgtcgaaa actacatttg gccctgcctg aaggctgacg ccaagtatga ggattacctg
     4381 ctgggtacct cccatgcgcg tccctgcatc gccgccgccc tggcggagat cgccaagaag
     4441 gagaacgccg acgccatctg ccacggctgc accggcaagg gcaacgacca ggtccgtttt
     4501 gagctgaccc tcaaggccct ggcccccgat atggccatca tcgccccttg gcgtgagtgg
     4561 agcatcaaga gccgtgacga ggagatcgac tacgccgagg cccataacat tcccctgaaa
     4621 atcaaccgtg agacgaacta ttccaaggat aagaacctgt ggcacctgag ccatgagggc
     4681 cttgaccttg aaaagccgtc cctggagccg cagtacaata agcccggctt cctggagctg
     4741 ggcgtttccc ccgagcaggc ccccgataag ccgacctacg tcaagatcca cttcgagaag
     4801 ggcatcccca ccgccgtgga cggcaaggag atggagagcg ttgagttggt cgagtacctg
     4861 aacaagctgg gcggcgcgaa cggcatcggc ctgctggata ttgttgagaa ccgtctggtc
     4921 ggcatgaaga gccgcggcgt ctacgagacc cccggtggtg ctatcctgta caaggcccat
     4981 gatgtcctcg agaccatcac cctggataag gagtccatgc acttcaaggc acagctggcc
     5041 cagaagatgg gcgagttggt ctacaacggc cagtggttca ccccgctgcg cgaggctatc
     5101 agcgccttca ccgacgacct gcagaaaacc gtcaccggcg acgtcaccct gaagctgtac
     5161 aagggcaaca tgatcaacgc cggcgtcacc agcccgtaca ccctgtacga tgagcagacg
     5221 gcctcctttg gtgaggatga tgattacaat caggcagacg ccaacggctt tatcaacctg
     5281 ttcggcctgc ccattgccga gcgcgccaag ctggccaaga actggcccac acaggactga
     5341 tttataagcg aacgaacgcc gaataaacaa ccgccaaggg gcgcggcaac cctttggcgg
     5401 ttttggtgtt actaaaaaag ataaaagata ataaaagatg tggagttttg gcctgaggcc
     5461 aaaacaaaaa aacgtagggg cggattccat atccgcccgt caacaggcag acgacctgcg
     5521 ggcgcatata gaatgcgccc ctacggaggc agaaaatttt tgagcggtgc gccggcaccg
     5581 cgaaccacca attttattat ctattatcta aaaaaatgga ggttctactt atgccccaac
     5641 tttggcaggg ccgggccagc aaggccgttg acagccgcgt aaacgatttt aactcttcca
     5701 tccgctttga cgcgcggatg atcgagcagg atattcaggg cagccttgtc cactcggcca
     5761 tgctgggcag acagggtatc atctcccaac aggatgtgga cgatatccac aaaggtctgc
     5821 actctatttt ggaggatctg cacagcggcg cactgatcgg tttcctttgg tacaatgctt
     5881 ttcccgccca agt
//
LOCUS       DBMDSQ010000106         5793 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_3447_length_5793_cov_2.089775, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000106 DBMDSQ010000000
VERSION     DBMDSQ010000106.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 5793)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5793)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5793
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3447_length_5793_cov_2.089775"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..604
                     /locus_tag="ACZPAX_05160"
     CDS             <1..604
                     /locus_tag="ACZPAX_05160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113321.1"
                     /translation="ESLGSDLFTNCRQLRVFTLRAAADAPTGLKKLLGAVSADITAEL
                     DGARLFYPEYSEFLDENTPAHIFNHSIEGEGYRMRQCFTPGGAVDYAAFDASFTQACV
                     GESADTLCRLALGRLVQPFGLSDNARADYELYLTAHPADAFKLAIDTRDETALRLLVG
                     LGLPTADAAVYCSRAGWSAGAAILLGRPRRVKKTYDFEDL"
     gene            607..1920
                     /locus_tag="ACZPAX_05165"
     CDS             607..1920
                     /locus_tag="ACZPAX_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HIR1113322.1"
                     /translation="MPKHIQTQTEWEQTMARRVMEQLRGELYLDQRYLTAALGALPAA
                     QTESGGSFATDGGALYYPAPWVLDTYRKNRRYLPRAYLHSLFHCIFRHLWLRDKRDPD
                     LWGLACDIAVESTLDALNTAATKRPVGWVRQQCYTQLRQSCKFLAAGPIYRVLTKTDA
                     ETLNKWQREFYTDSHRLWPADPTSPEAQMRGKQWENLGRQTELSIEENGQRAGQDTAA
                     QALQAQVQAGRSRRTYRDFLRRFAVWHEEPHLDPEEFDLGFYSYGLRTYGNMPLIEPL
                     ESREVKKIRDFVIVVDTSESTAGELVKAFLKETFTLLKSQDSFFRQCRILVMQADNAV
                     RDETWLNDLDALDRYTAQFTLVGGGGTDFRPAFARIEELRQDGALRDLQGVLYFTDGK
                     GVYPTRRPVFDVAFLFLEDGTAPPDVPPWAMRLVLQPEEFDPKGR"
     gene            1924..3459
                     /locus_tag="ACZPAX_05170"
     CDS             1924..3459
                     /locus_tag="ACZPAX_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR1113323.1"
                     /translation="MDIQRAKEEIKRSVQAYLATDDIGRPLIPPIRQRPILLMGPPGV
                     GKTQIMEQIAQECDIALVAYTITHHTRQSAVGLPFIRERDFGGKTRSVTEYTMSEIIA
                     SVYAAMERTGKKNGILFIDEINCVSETLAPTMLQFLQCKTFGNQAVPEGWVIVAAGNP
                     PEYNKSVREFDLVTLDRVRRIDVEPKLSVWQDYARAHRLHPAVTAYLELRPQHFYKIE
                     NDVDGVQFVTARGWEDLSAFLQAADKLDLPIDEGVIGQYLRHPEVARDFAAYWVLYRK
                     YHQDYGVEDILQGKPFDTVVARAMDASFDERISLVSLLLAGLNTRFAAATATGAVTDA
                     CYQQLRSFKRTLTQQDDADPAALFAAQCENYRARLDTDKAAGALLPDEAAARTRTLAL
                     LEEWSRHLDSALDADDAFDTVRGAFNAQVRHREEAVSTAGDALECAFDFMEQAFEDGQ
                     EMVVFVNELALGPDSAPFLAENECERFEQYSEKLLLHGGEDELLAELQRDDVRGAEHS
                     AEF"
     gene            3693..3962
                     /locus_tag="ACZPAX_05175"
     CDS             3693..3962
                     /locus_tag="ACZPAX_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015563657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HIR1113324.1"
                     /translation="MKAVITVIGRDTVGVVAKVSAVCAELNINIEDVSQSIMQEMFCM
                     IMLVDLSKSTAEAAAVRDRFAELGKEMKMQVTVTRQEVFDAMHTI"
     gene            3975..5342
                     /locus_tag="ACZPAX_05180"
     CDS             3975..5342
                     /locus_tag="ACZPAX_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007045965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF711 family protein"
                     /protein_id="HIR1113325.1"
                     /translation="MRIINSRDIMETIEMLTAENLDVRTVTMGISLLDCIDPDGDKAC
                     EKIYNKIVRLAGNLVPVVDGISAEYGIPIVNKRISVTPIAMLLGAAPDADPVQYAKAL
                     DRAAKAVGVNFIGGFGALVHKGFSAGDKRLIEAIPRALAETDIVCSSVNIGSTKSGIN
                     MDAVKLMGQVVRETAELTKDNMCMGDAKLVVFCNAPEDNPFMAGAFHGAGEPDCEIHV
                     GVSGPGAVRAALAKLPKDAPMDEVAELVKRTAFKITRLGQLVANLASERLGVPAGIID
                     LSLAPTPAIGDSVANILEEMGLESCGCCGTTACLALLNDAVKKGGVMASNHVGGLSGA
                     FIPVSEDDGMINAANCGSLTLEKLEAMTAVCSVGIDMVVIPGDTTAEVISALIADEAA
                     IGMVNSKTTAVRVIPAIGHKAGDVLDFGGLLGHAPIMPISKFSPAVMIHRGGRIPAPM
                     QALKN"
     assembly_gap    5402..5411
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1182 a         1716 c         1791 g         1094 t
ORIGIN      
        1 ggaatccttg ggcagcgatt tgttcacgaa ctgccgccag ttacgggtgt ttacgctgcg
       61 ggccgcagcg gacgccccca ccgggctgaa aaagctgctg ggggccgtca gcgccgatat
      121 cacggcagaa ttggatggtg cgcggctgtt ttaccccgag tattcggagt ttttggacga
      181 gaacaccccc gcgcatattt tcaatcacag cattgagggc gagggctacc ggatgcggca
      241 gtgctttacg cccggcggcg cagtagatta cgcggcgttt gacgcatcgt tcacgcaggc
      301 ctgcgtcggc gaaagcgcag atacgctctg ccgcctggcg ctggggcggc tggtgcagcc
      361 gttcggcctt agcgacaacg cccgcgcaga ctatgaattg tatctgaccg cccacccggc
      421 ggatgcgttc aagttggcca tcgacacccg ggacgaaacc gcgctgcggc tgctggtggg
      481 cctaggcctg cccaccgctg acgccgccgt ctattgttcc cgcgccggtt ggagcgccgg
      541 ggcagccatt ttgttgggcc gccccaggcg ggtgaaaaag acctatgatt ttgaggattt
      601 gtaaaaatgc ccaaacacat ccaaacccaa accgaatggg aacagacgat ggcccgccgg
      661 gtcatggaac agctgcgcgg ggagttgtat ctggatcagc gctacctgac ggcggcgctg
      721 ggggcactgc ccgccgcaca gacggagagc ggcggcagtt ttgccaccga cggcggggcg
      781 ctctactacc ctgcgccctg ggtgctggac acctaccgca aaaaccgccg ctatctgccc
      841 cgggcctacc tgcacagttt gttccactgc atcttccgcc acctgtggct gcgggacaag
      901 cgcgatcccg acctgtgggg cctggcctgt gacattgctg tggaaagcac gctggacgcc
      961 ctgaacaccg ccgccacgaa gcgcccggtg ggctgggtgc ggcagcaatg ttatacacaa
     1021 ctgcggcagt catgcaaatt tctggccgcc gggccaatct accgtgtgct gactaagacc
     1081 gacgccgaaa cgttgaacaa gtggcagcgg gagttttaca ccgacagcca ccgcctgtgg
     1141 cctgccgacc ccaccagccc cgaggcccag atgcgcggca agcagtggga aaacctgggc
     1201 cgccagaccg aattgagcat agaggaaaac ggccagcgcg ccgggcagga cactgccgcc
     1261 caggctttgc aggcccaggt gcaggccggg cgcagccgcc gcacctaccg cgacttcctg
     1321 cgccgcttcg ccgtctggca tgaggagccg caccttgacc ccgaggaatt tgacctgggc
     1381 ttttacagct acggcctgcg cacctacggc aatatgccct taattgagcc gctggaaagc
     1441 cgcgaggtga aaaagatccg tgacttcgtc atcgtggtgg acaccagcga gtccaccgcg
     1501 ggcgagctgg tcaaggcatt tttgaaagag accttcacct tgttaaaaag ccaggacagc
     1561 tttttccgcc agtgccgcat tttggtcatg caggcggaca acgccgtgcg ggacgaaacc
     1621 tggctgaacg atttggacgc cctcgaccgc tacacggccc agtttacgct ggtgggcggc
     1681 ggcggaacgg acttccgccc cgcattcgcc cgcattgaag aactgcgcca ggacggcgca
     1741 ctgcgagact tgcagggtgt tttatacttc accgacggca agggtgtcta tcccacacgc
     1801 cgcccggtgt tcgatgttgc gtttttgttt ctggaggacg gcaccgcccc gcccgatgtg
     1861 ccgccctggg ccatgcgcct tgttttgcag ccggaagaat ttgacccgaa aggacgatga
     1921 accatggata ttcaacgtgc caaagaggaa atcaagcgtt ctgtgcaagc ctaccttgcc
     1981 acggacgata ttggccgccc gctgataccg cccatccgcc agcgccccat tttgctgatg
     2041 ggcccgccgg gcgtaggcaa aacccaaatt atggagcaga ttgcgcagga gtgcgacatt
     2101 gcgctggtgg cctataccat cacccaccac acgcggcaga gtgccgtggg cctgcccttc
     2161 atccgggagc gggattttgg cggcaagacc cgcagcgtga ccgagtacac gatgagtgag
     2221 atcatcgcca gcgtctacgc ggccatggag cgcaccggca agaaaaacgg tatcctgttc
     2281 atcgacgaga tcaactgcgt cagcgagacg ctggccccga cgatgctgca atttttgcag
     2341 tgcaagacct tcggcaacca ggccgtgccg gaaggctggg tcattgtggc ggcgggcaac
     2401 ccgccggagt acaacaagag tgtgcgggaa tttgacctcg tcacgctgga ccgcgtgcgg
     2461 cgcatcgacg ttgagccgaa gctgagcgtc tggcaggact acgcccgcgc ccaccggctg
     2521 caccccgccg tgacggccta tctggaactg cgcccccagc acttttacaa aatcgagaac
     2581 gatgtggacg gcgtacagtt tgtgaccgcc cgcgggtggg aggatctgtc cgccttcctg
     2641 caggcggcgg acaagctgga tctgcccatc gacgagggcg tcatcgggca atacctgcgc
     2701 cacccggagg tcgcgcggga ttttgcggcc tactgggtgc tctaccgcaa gtaccaccag
     2761 gactacggcg tcgaggatat cctgcagggc aagccctttg acacggtggt ggcccgcgcc
     2821 atggacgcga gctttgacga gcgcatcagc ctggtcagcc tgttgctggc ggggctgaac
     2881 acccgctttg ccgccgccac ggccaccggc gcggtgacgg atgcctgcta ccagcagctg
     2941 cgcagcttta agcgcacact gacccagcag gatgacgccg accccgctgc gctgtttgcc
     3001 gcccagtgcg agaattaccg cgcccggctg gacaccgaca aggctgccgg ggcactgctg
     3061 cccgatgaag ccgccgcccg cacccgcacg ctggccctgc tggaagagtg gagcaggcac
     3121 ctggacagcg ccctggatgc tgacgacgcc tttgacacgg tgcgcggggc gttcaatgcg
     3181 caggtgcgcc accgggagga agcggtttcc actgcgggcg acgcgctgga atgcgccttt
     3241 gactttatgg agcaggcgtt tgaagacggc caggagatgg tcgtgtttgt caacgagttg
     3301 gccctcggcc ccgactccgc gccgttcttg gccgagaacg agtgtgagcg gtttgagcag
     3361 tacagcgaga agctgctgct ccacggcggc gaggatgaac tgctggccga attgcagcgg
     3421 gatgatgtac gcggcgcgga acattcggcg gagttttgac atttccgacg ccctgtaggg
     3481 gccgggcatg accggctcgc gcgtgaaagg caagcggatg tccgccgtac agttgcggac
     3541 cacacctgtg cggcccctgc aaagcggcag cttgttggct gttcgctcat ttgaaaaagt
     3601 atcacttttc gttaaattct ttactctttt gcagaacttt gttataatgg cagtattccg
     3661 tttatcaccg gacattttga gggggtcata ctatgaaagc agttatcacg gtcattgggc
     3721 gggacaccgt gggtgtcgtt gccaaggtca gcgcggtgtg tgccgagctg aatatcaaca
     3781 ttgaggacgt ctcccagtcc atcatgcagg agatgttctg catgatcatg ctggtggatc
     3841 tgagcaaatc gaccgccgag gccgccgctg tgcgcgaccg cttcgccgag ctgggcaaag
     3901 agatgaagat gcaggtcact gtgacccgcc aggaagtctt tgacgccatg cacaccattt
     3961 aagggggcgg cagtatgaga atcatcaaca gccgcgacat tatggaaacc atcgagatgc
     4021 tcaccgcaga aaatctcgat gtgcgcaccg tcaccatggg catcagtctg ttggactgca
     4081 tcgacccgga cggcgacaaa gcctgcgaaa aaatctacaa caagatcgtc cgtctggcgg
     4141 gcaatctcgt cccggtcgtg gacggcatca gcgccgagta cggcatccct atcgtcaaca
     4201 agcgcatcag cgtcacgccg attgccatgc tgctgggcgc tgcgcctgat gccgacccgg
     4261 tgcagtacgc caaggcgctg gaccgcgcgg ccaaggccgt gggggtcaac ttcatcggcg
     4321 gcttcggcgc gctggtacac aagggctttt cggcgggcga caagcgcctg atcgaggcta
     4381 tcccccgtgc gctggccgaa accgatattg tatgttccag cgtcaatatc ggctccacca
     4441 agtccggcat caatatggac gccgtcaaac tgatgggcca ggtcgtgcgc gagaccgccg
     4501 agctgaccaa ggacaatatg tgcatgggcg acgcaaaact cgtcgtgttc tgcaatgcgc
     4561 ctgaggacaa ccccttcatg gcgggcgcgt tccacggcgc gggcgagccg gactgcgaga
     4621 ttcacgtcgg cgtttccggc cccggtgcgg tgcgtgccgc gctggccaaa ctgccgaagg
     4681 acgccccgat ggacgaggtg gccgagctgg tcaagcgcac ggcgttcaaa attacccgcc
     4741 tgggccagct ggtggcaaat ctcgccagcg agcgtctcgg cgtgcctgcg ggcattatcg
     4801 acctgtcgct ggccccgacg cccgccatcg gcgacagcgt tgcgaacatt ctggaagaga
     4861 tgggcctgga aagctgcggc tgctgcggca ccacggcctg cctggccctg ctgaacgacg
     4921 cggtcaagaa gggcggcgtc atggcgtcca accatgtcgg cggcctgtcc ggcgcgttca
     4981 tcccggtgtc ggaggacgac ggcatgatca acgccgccaa ctgcggcagc ctgacgctgg
     5041 aaaagctcga agccatgacg gcggtctgct cggtcggtat cgacatggtg gtcatcccgg
     5101 gcgataccac cgccgaggtc atcagtgccc tgattgcgga tgaagccgct atcggcatgg
     5161 tgaacagcaa gacgacggcg gtgcgcgtca tccccgccat cggccacaag gccggcgatg
     5221 tgctggactt tggcggcctg ctgggccacg cgcccatcat gcccatcagc aagttcagcc
     5281 ctgcggtcat gatccaccgc ggcggccgca tccccgcccc gatgcaggcg ctgaaaaact
     5341 aaagcagtaa aggcagcgcc catgccgggg atgcggccgc cgcggtggat catgaccgca
     5401 gnnnnnnnnn nattgatgga ggaagcaaac agggaaatct gtcggttggg cgatttttca
     5461 tggtttactt agttgggtgt atgtcatata tttccttttg cgttactaaa ttcatgcttc
     5521 acggattatg atattgcacc tgcaattaaa aattgatatt atggtaaata atgagtctga
     5581 attacatgtg cttctcattc tagattgatg tatgcttctc aactacactt gaaaaaacag
     5641 ttgatacaca gagaaatact gtaataacaa acttggttaa gaaaaattat ttcatgagtc
     5701 aggttgacaa atatatttat aagtacgatg taaataaaaa tgaaggttat atcttgggag
     5761 atttagggaa aaatcgacaa aagtgtggaa tgt
//
LOCUS       DBMDSQ010000107         5714 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_3490_length_5714_cov_1.609834, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000107 DBMDSQ010000000
VERSION     DBMDSQ010000107.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 5714)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5714)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5714
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3490_length_5714_cov_1.609834"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..625)
                     /locus_tag="ACZPAX_05185"
     CDS             complement(<1..625)
                     /locus_tag="ACZPAX_05185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113326.1"
                     /translation="MLTNLLKYEFKASGRVVLPIASGVLVLNIFTGILSHFIQNTSDR
                     FPLAGLALVLLNLAALVSLLVVLAVCFFIEVQQFYRLLGDRGYLMLALPVQPWKHIAA
                     KVICGTAWTVFGMLFFSVCCALLTGDFAFSVPHAEDFAIWAVMVLIVLALIAGALLHT
                     YLACAFAGQFTQQRILISIAAYFVIGFAGQMAALVTAFFVALYGYQYF"
     gene            complement(619..1317)
                     /locus_tag="ACZPAX_05190"
     CDS             complement(619..1317)
                     /locus_tag="ACZPAX_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015565566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1113327.1"
                     /translation="MDAIYEVHGLTKRYGRTTALDGAEFSVTPGKLVGLLGPNGSGKT
                     TLLKISAGLLTPTAGSVLVNKTPVGVYTKAVTSYLPDRMALPTEFTAADAVSLYNDFF
                     PDFDKAKADAMLADLRIQAHDKIGTMSKGTQEKMQLCLTMSRAAKLYLLDEPLGGVDP
                     AARDYILQTILHNYSEDAALVLSTHLIGDIEKVLDEVIFLQNGKALRQAAVDELREET
                     GMSVDAYFREVYKC"
     gene            complement(1321..1695)
                     /locus_tag="ACZPAX_05195"
     CDS             complement(1321..1695)
                     /locus_tag="ACZPAX_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR1113328.1"
                     /translation="MNWNITAGRPVYVQLVEQLELALVAGEFPPGSRIPPVRELAADA
                     GVNPNTMQRALQELESRGLLQAQRTAGRTVTADDAALHALRRRRAQTLAGEFLHQMHA
                     LSLTETEIETLLRETAQQQTEE"
     gene            complement(1824..2756)
                     /locus_tag="ACZPAX_05200"
     CDS             complement(1824..2756)
                     /locus_tag="ACZPAX_05200"
                     /inference="COORDINATES: protein motif:HMM:NF023568.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR1113329.1"
                     /translation="MSDLHCETITFPSSDGRHTSSAVLYTMQDKPVRAVIQLSHGMCE
                     YVRRYAPMAEFYAAHGIALAGNDHLGHGDTAKAGEHGHYGEPDGRLHLLNDLHTMNGL
                     LHERFPDTPIILYGHSMGSFYARWYAEKWPESITALVISGTAGPSFMNVIGQKLAGLI
                     ARVKGPRYVSPLMVKLNFGSYCKKIANAQSPNAWLSRDENVVNAYDADCLCTFQFTAA
                     TYREMLTTLNHVSTKAWAQAIDKDLPILLIAGDGDPVGGYGSGVRKVWAMLGDAGVND
                     LTCQIFEGGRHELHNETNRDEVFDYVLTWIEDHI"
     gene            complement(2775..3116)
                     /locus_tag="ACZPAX_05205"
     CDS             complement(2775..3116)
                     /locus_tag="ACZPAX_05205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113330.1"
                     /translation="MALAWAYGLYWFPFILIAPGPCIVLSLLAACLDKVAPQIIRGCT
                     FLGGCTLEIYAVHAFAFQVFRDALSDDILYGTDKRWLLLSVLSVLFAVLLARVMAQLT
                     AKTQKQPAQQT"
     gene            complement(3477..4739)
                     /locus_tag="ACZPAX_05210"
     CDS             complement(3477..4739)
                     /locus_tag="ACZPAX_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HIR1113331.1"
                     /translation="MEPLAQRLRPRSLDEVCGQQHLLGKNQVFRRTVESGKIPNMIFY
                     GPSGVGKTTVASIIAAGSGMQLHKLNGTTASTSDIKSVLADIGTLGAAGGILLYLDEI
                     QYFNKRQQQSLLECVEQGTVTLIASTTENPYFYVYNALLSRCTVFEFKSLTADDIRVG
                     LQNAAARLGAEDKTPILIPDDALDYLAQSAGGDMRKALGNLEFAVTAAPIENGSRTIT
                     LDMVQQVAVRTAMRYDKLGDDHYDIVSAYQKSMRGSDPDAALHYLGRLLEAGDLPSAC
                     RRLMVCACEDVGLAWPQIIPIVKAAVDIANAVGLPEARLPLADAVVLVATAPKSNSAH
                     DGINAAIADIQAGRTGPIPRQLQNKHYDGADAKVKGQHYLYPHDYPNHWVAQQYLPDA
                     IKDRQYYVPGENKNEQAFAQYWKKIKGE"
     gene            4861..5508
                     /locus_tag="ACZPAX_05215"
     CDS             4861..5508
                     /locus_tag="ACZPAX_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III domain-containing protein"
                     /protein_id="HIR1113332.1"
                     /translation="MTKQELAAVCIARLKAEYPDSDCTLDYDHAWQLLVSVRLAAQCT
                     DARVNVVVQDLFAKYPGVKELAAASPEEIEAIVKPCGLGHSKARDISACMRVLRDQYD
                     CHVPDDFNALLALPGVGRKSANLIMGDVFGKPAIVTDTHCIRLCNRIGLVDNVKEPAK
                     VEKELWKIIPPEEGNGFCHRLVDHGRAVCTARTKPYCDKCCLADVCRARKEFANE"
     gene            5501..>5714
                     /locus_tag="ACZPAX_05220"
     CDS             5501..>5714
                     /locus_tag="ACZPAX_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005934447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HIR1113333.1"
                     /translation="MNKTTESRLGGELRSQQASQRLLDELRGGRYVKATQLPSELELA
                     DVLGVSRTVVRDALSDLERDGYVERVR"
BASE COUNT         1249 a         1571 c         1734 g         1160 t
ORIGIN      
        1 tgaaatattg gtatccatag agggcaacga aaaacgcagt taccagtgcc gccatctgcc
       61 cggcaaaacc gatgacgaag taagcggcga tgctgataag aatgcgctgc tgggtgaact
      121 gcccggcgaa agcacaggcc aggtaggtgt gcagcagagc ccccgcaatc agggccagaa
      181 cgataagcac cattacagcc cagatggcaa aatcctcagc atgagggacg ctgaatgcaa
      241 aatcgcctgt aagcagtgca cagcagacgg aaaaaaataa cataccgaac acggtccacg
      301 ccgtgccgca aataaccttg gcggcaatat gcttccaggg ctgcacgggc agggccagca
      361 tcaggtagcc gcggtcgccc agcaggcggt aaaattgctg cacttcaata aagaagcaga
      421 ccgctagtac taccagcagg ctgacgagcg ctgccaggtt cagcaacacc agggccagcc
      481 cggccagcgg aaatctgtca gaggtgttct ggataaaatg gctcaggata ccggtgaaaa
      541 tgttcagcac cagcacaccg cttgcaatcg gcaggacaac gcggcccgag gccttgaact
      601 cgtatttcaa taaatttgtc agcatttgta gacctcccgg aagtatgcat caacgctcat
      661 gcctgtttcc tcgcgcagct cgtcaacggc ggcctggcga agggccttgc cgttttgcag
      721 gaaaattacc tcatccagca ccttttcaat gtcgccaatc aggtgggtgg acaggaccag
      781 cgcggcgtcc tcgctgtaat tgtgaaggat cgtctgcaaa atgtagtcgc gggcagcggg
      841 gtcaacgccg cctaagggtt catccagcag gtagagcttg gcggcgcggc tcatggtcaa
      901 gcagagctgc attttctcct gcgtcccctt gctcatggta ccgattttgt cgtgagcttg
      961 gatgcgcagg tcagccagca tggcgtcggc cttggccttg tcaaaatcgg ggaaaaagtc
     1021 attgtacagg ctcacggcgt cagcggctgt gaactcagta ggcagggcca tccgatccgg
     1081 cagatagctg gtgacagcct tggtgtagac accgacgggg gtcttattga ccagcacact
     1141 gcccgctgtg ggcgtaagca gcccggcaga gattttaagc agtgtggttt tgccgctgcc
     1201 gttggggccc agcagcccga ccagcttgcc gggcgtgacg gaaaactctg cgccgtccag
     1261 ggcggtggtg cggccatacc gtttggtcag gccgtgtacc tcataaatag catccatggt
     1321 ttactcctct gtctgctgct gtgcggtttc gcgcagcagc gtttcaattt cggtttcggt
     1381 caggctcagg gcgtgcattt gatgcaggaa ctcccctgcc agcgtttggg cgcggcggcg
     1441 gcgcagggcg tgtagcgcgg cgtcatcggc ggtcacggtg cggcctgccg tgcgctgggc
     1501 ctgtagcagg ccgcggcttt ccaattcctg caaggcacgc tgcatcgtgt tgggattgac
     1561 gcccgcgtcg gcggccagct cccgcacagg cgggatgcgg ctgccgggcg ggaactcccc
     1621 tgccaccagc gcaagctcaa gctgttccac aagctgcacg tagaccgggc ggcctgctgt
     1681 gatgttccag ttcataacgg agactccttt gctgtatcat tgtgttaaga acttaataca
     1741 atgatacaac tacaatatgg atttgtcaag aggaaaaata aaaatcctcc accaatgagg
     1801 cggaggatgc gatacagcgt ggtttaaatg tgatcttcaa tccaggtcag cacgtagtca
     1861 aagacctcgt cgcggttggt ctcgttgtgc agttcgtggc ggccgccctc aaaaatctgg
     1921 caggtcaggt cgttgacacc ggcgtcgccc agcatggccc agaccttgcg cacgccgctg
     1981 ccgtagccgc ccaccgggtc gccatcaccg gcaatcagca gaatgggcag gtctttgtcg
     2041 atggcctgcg cccacgcctt agtgctgaca tggttcagcg ttgtcagcat ttcgcggtag
     2101 gtggcggcgg tgaactggaa ggtacacagg cagtcggcat cgtaggcgtt cacaacgttt
     2161 tcgtcgcggg aaagccaggc gttgggcgat tgggcgtttg cgattttctt gcaatagctg
     2221 ccaaaattca gcttgaccat cagcggcgaa acatagcgcg gccccttgac gcgggcaatc
     2281 agcccggcta gtttttggcc gatgacgttc ataaagctgg ggcctgccgt gccggaaata
     2341 accagtgccg tgatgctctc cggccacttt tcggcgtacc atcgcgcgta aaagctgccc
     2401 atgctgtggc cgtacaggat aatgggcgta tccgggaaac gctcatggag caagccgttc
     2461 attgtatgca ggtcgttcag cagatgcagg cggccatccg gctcgccgta gtggccgtgc
     2521 tcccctgcct tggcggtgtc gccgtggccg aggtggtcat tgcccgccag tgcaatgccg
     2581 tgggcggcgt agaactcggc catgggcgcg taacggcgca catactcgca cataccgtgg
     2641 ctcagctgga tgactgcccg cacaggctta tcctgcatcg tgtacagcac agcggaggaa
     2701 gtatggcgtc cgtccgacga cggaaacgta atggtctcgc agtgaaggtc agacataaat
     2761 ttctcctatt tcagtcaggt ttgttgggcg ggctgctttt gtgttttggc ggtcaattgc
     2821 gccattacgc gggcaagcaa aacggcaaag agcacagaca ggacggaaag cagaagccag
     2881 cgcttgtccg tgccatacaa gatgtcgtcc gagagtgcat cgcgaaagac ctgaaaagca
     2941 aaagcgtgaa cagcgtaaat ttccagtgtg catccgccca ggaacgtaca gccgcggatg
     3001 atttgcgggg caactttatc caggcaggcg gccagcaggg acagtacaat acacggccca
     3061 ggcgcaatca ggataaacgg aaaccaatac agaccatagg cccaagccaa ggccaaataa
     3121 actgtctact cctaagtaag tggcgaccac accctgcacc gtggtaccta ggcgcaggcc
     3181 gtcaaaaatg acccaaggcg tgacacagag ataataggca ggtacaatct tgataagacg
     3241 ctttttaata aagatgagcg tatcagggtt ctttttcagc gagtgatagc acccgagtcc
     3301 tgaggcaaac acaaaaatgt caacaccgcc tgccccgcag ctttttaaga cctgcaaaaa
     3361 ctcacagggg ggggtaagcg aaatatggtg atataccacc cacaaggcag caagggccat
     3421 ccacacactg cggtaggata gaattttttt gaagtcgcaa aagtcagcca cagcggttat
     3481 tctcccttga ttttcttcca atattgggcg aaggcctgct cgttcttgtt ctcgccgggg
     3541 acgtaatact ggcgatcctt gatagcatcg ggcaggtatt gctgggcgac ccagtggttg
     3601 gggtaatcgt gtgggtagag gtaatgctgc cccttgacct tggcatccgc gccgtcgtag
     3661 tgtttgtttt gcagctggcg cgggatgggc ccggtgcgcc cggcctggat gtccgcaatg
     3721 gcggcgttga tgccatcgtg ggcggagttc gacttgggcg cggtggcaac cagcacgacc
     3781 gcatctgcca gcggcaggcg ggcctccggc aggccaacgg cgttggcgat atccaccgcc
     3841 gctttgacaa tggggataat ttgcggccag gccaggccga catcctcaca cgcgcagacc
     3901 atcaggcggc ggcaggcgct gggcaggtcg cccgcttcca gcaggcggcc caaataatgc
     3961 agcgctgcgt cggggtcaga gccgcgcatg gatttctggt aggcagagac gatatcgtaa
     4021 tggtcgtcgc ccagtttatc ataccgcatg gcggtacgca cggccacctg ctgtaccatg
     4081 tccagcgtga tggtgcggct gccattttca atcggcgcgg cggtcacggc aaattccagg
     4141 ttgcccagcg ctttgcgcat atcgccgccc gcgctctggg ccaggtagtc cagcgcatcg
     4201 tcgggaatca agataggcgt tttatcctcg gcccccagcc gcgcggcggc gttctgcaag
     4261 ccgacgcgga tatcgtccgc cgtcagcgat ttgaactcaa acaccgtgca gcgggataaa
     4321 agggcgttgt atacataaaa ataggggttc tcggtcgtgg aggcaatcag ggtgacggtg
     4381 ccctgctcca cgcattccag caggctttgc tgctggcgct tgttgaaata ctggatctcg
     4441 tcaagataca gcaagatgcc cccggccgcg cccaaggtac cgatatccgc cagcactgat
     4501 ttgatatcgc tggtggaggc cgtcgtaccg ttgagcttgt gcagctgcat accgctgccc
     4561 gccgcaatga tgctggcaac cgtcgttttg ccaacaccgg aagggccgta gaagatcatg
     4621 ttggggatct tgccgctctc gaccgtgcgg cggaatacct gatttttgcc gagtagatgc
     4681 tgctgcccgc agacttcatc caggctgcgg gggcgcaggc gttgtgccaa tggctccatt
     4741 tcgtgttcct tttttaagat aagtttgaga tagtgtacca aaacgtcata cttctgctct
     4801 tgattatatc aaacacacgt ggtaaaatca aatgaaaaga acagaaacgg agaaaaagca
     4861 gtgacgaaac aagaattggc ggcagtctgc attgcacggc tgaaagcaga ataccccgac
     4921 tctgactgca cgctggatta tgaccatgcg tggcagcttt tggtatcggt ccggctggcg
     4981 gcccagtgta ccgatgcccg cgtgaatgtg gtggtgcagg atctgttcgc caagtatccc
     5041 ggggtgaaag aactggctgc ggccagcccg gaggaaatcg aggccattgt caagccttgt
     5101 gggctggggc acagcaaggc gcgggatatt tcggcctgta tgcgggtgct gcgcgaccaa
     5161 tatgactgcc atgtgcccga tgattttaat gcgctgctgg cgctgcccgg cgtgggccga
     5221 aagagcgcaa acctgattat gggcgatgta ttcggcaagc ccgccattgt gacggacacc
     5281 cattgcatcc gcctgtgcaa ccgcatcggg ctggtggata atgtcaaaga acccgccaag
     5341 gtcgaaaaag agctgtggaa aatcatcccg cccgaggaag gcaacggctt ctgccaccgg
     5401 ctggtggacc atggccgtgc ggtctgcacg gcccgcacca agccctactg cgacaaatgc
     5461 tgcctggctg acgtatgccg ggcacgaaag gaatttgcga atgaataaga cgacggaaag
     5521 ccgccttggc ggtgaactgc gcagccagca ggcctcccag cgtttgttgg atgagctgcg
     5581 cggcggccgc tatgtcaaag ccacccagct gcccagtgaa ttggaattgg ccgatgttct
     5641 gggcgtgagc cgtaccgtgg tgcgcgacgc cctgagcgat ttggagcgtg acggctatgt
     5701 ggagcgcgtg cgcg
//
LOCUS       DBMDSQ010000108         5710 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_3493_length_5710_cov_2.183990, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000108 DBMDSQ010000000
VERSION     DBMDSQ010000108.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 5710)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5710)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5710
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3493_length_5710_cov_2.183990"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            652..1209
                     /locus_tag="ACZPAX_05225"
     CDS             652..1209
                     /locus_tag="ACZPAX_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005806368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR1113334.1"
                     /translation="MNTIVTSKEEILKTSRALIQEQGWSAVSIRSVAAACGVSVGSIY
                     NYFDSKAELVSATVESVWREIFHRPEDAAVFQDVQACVIWMYERMAYGYEQYPGFFTL
                     HSLGFMRDEKADGKRHMQQAWHHMLMGLCAVLKQDARIRPDAFDEQFTAEKFAEVLFS
                     QLLSALLRQDYDPSAVLEVVRRTLY"
     gene            1316..1981
                     /locus_tag="ACZPAX_05230"
     CDS             1316..1981
                     /locus_tag="ACZPAX_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005940225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113335.1"
                     /translation="MQAIVETVFDAVYLVSVITIGILMIRGSKGNPQFRLFGLMAVVL
                     GAGDSFHLIPRALALCTTGLENYTVPLGLGKWITSVTMTIFYVLLYYVWRQRYRVEGR
                     KSLTAAVYGLAAVRVILCMMPQNQWLSTESPLSWGIYRNIPFALMGLLIIVLFFRSAR
                     EHNDRAFRWMWLTIVLSFGFYIPVVLWADAIPMIGMLMIPKTCAYVWTVLIGYSAMKK
                     ECR"
     gene            2214..2519
                     /locus_tag="ACZPAX_05235"
     CDS             2214..2519
                     /locus_tag="ACZPAX_05235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113336.1"
                     /translation="MNYLDCAQYDCCHNKAGCCCLNSINVRHTGPGDAVCGSYRNSEG
                     YGNVATDNQPASPETDIHCDDKGCRHLEGRCCCADHVSIDERGCGPACATRAPRTAE"
     gene            2761..3876
                     /locus_tag="ACZPAX_05240"
     CDS             2761..3876
                     /locus_tag="ACZPAX_05240"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007045733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HIR1113337.1"
                     /translation="MSETPITVPFVSFRPMERELDAELRGAFARVLDNSWYIGGREDA
                     AFEKAFAEYCGVKHCIGCGNGLDALVLILKAYGIGTGDEVIVPSNTFIATVLAVSYAG
                     ATPVLVEPTLESYNIDPARIEAAVTPRTKAIMAVHLYGQCAPMDEINAIAKAHGLKVI
                     EDAAQAHGAEYKGRRAGSLGDAAGFSFYPGKNLGALGDAGCVTTNDDELAERVRALGN
                     YGSDYKYHHIYKGQNSRLDELQAAFLAAKLPHLDTMNAERRRIAARYLAEMHNPAVTL
                     PAELPGCTHVWHIFAVRCADRNALEKHLNARGIGTNKHYPTPIHLQGAYAELGIPKGA
                     LPLAEEISATELSLPMFYGMTDAQVQAVIDAVNDFKG"
     gene            3880..4767
                     /locus_tag="ACZPAX_05245"
     CDS             3880..4767
                     /locus_tag="ACZPAX_05245"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR1113338.1"
                     /translation="MPKVGIVISNYNGWQDTLVCLESLQKMTFRDFEIVLIDDASPND
                     SVAQLQGKLPPNTVFLPQEKNVGFAAANNIGMRRALADGCDYALLLNNDTSVHPDFLE
                     KLLAETPEGAVSCPKMLFMDPPDEIWFAGGELDPATGKVKHLGGHKKDGPEFAEKRQV
                     SFITFCCVLLPRAVIEKVGYLDETLFMYCEDVDYCIRLAQVGVPMWFLPDAVIHHKAG
                     GSAGGMLSVYYITRNTLYLTCKGKSAAYKKLKTVIPILTGAARYALTKALGRKKGRSY
                     GAYRGAVDFWQGKMGRMEG"
     gene            4933..>5710
                     /locus_tag="ACZPAX_05250"
     CDS             4933..>5710
                     /locus_tag="ACZPAX_05250"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR1113339.1"
                     /translation="MPQLTIVILQYRPDAAALRRTLASLAMQDTRDFAVVLGDDGSQQ
                     DYFAESRAYLAAHGITDVQTAKLIPNGGTVKNAQNALRTAATRWVLMLSPGDFLYDSE
                     TVSWWLGRLQADGPRVAFGKQAYFIPGPAPQPTPGETPFDRTPYDPAHYDAKTIRRNL
                     LLYDDGISGAGLVYERTLFLSALDKMADHVRLAEDFSLRLFAVQGVRITRYDRLTVWY
                     EYGGGVSTDAAARERMLGDWRAMLEVLRAAYPHDRTVRLAY"
BASE COUNT         1194 a         1677 c         1667 g         1172 t
ORIGIN      
        1 ttaatcttct gcatttgtga gtggattcta catagtcaag taacgctaat tgctattcag
       61 ttacaacgaa atatttagat agcagccgcc ctcaatgcga ggacggctgc tttactactt
      121 tctgttggta taataattaa gctgtcagtt atgactgctt ctattttgcc ccaataagtg
      181 atgaggatga ctgtataatt gcccttaaag cggttgcgag atgatacata gaatgatata
      241 attaagtcat cgaaagggaa aaacgaatgt atatatattc gaaatgcctt ttctgatatg
      301 tgacctatgc aaagttcgaa agaactgcgc aggtaaggga tttgtagagg gctctatcgt
      361 acagtgtttg atggtatctg ctatcttaca tattcccaac ggagtgtgaa cagaaaacag
      421 ttcacctatg aaattcaccc atagaaaaca acgtagctaa aacagcttac gaaagttctc
      481 tatgggtgga tttttttgcg gtaatgcggt gcgccgtgct ctgcgtagcc gggccttcac
      541 agtgaaaagt acgaaaaaag ttcacaaaaa acttttccgt tcctattgac agggggcagt
      601 gcgaagctat aataaaagtt gtaagtgaac aatgttcatg ttgaggtgcc catgaatacg
      661 atcgtcacat ccaaagagga gatcctgaaa accagccgtg cgctgatcca ggagcagggg
      721 tggtctgctg tcagcatccg ctctgttgcg gcggcgtgcg gggtatcggt aggttctatt
      781 tacaactatt ttgactccaa ggcggagctg gtcagcgcta ccgttgaaag cgtctggcgc
      841 gagatcttcc accgcccgga ggatgccgct gtctttcagg atgtgcaggc ctgcgtgatc
      901 tggatgtatg agcgcatggc ctacggctat gagcagtatc ccggcttttt cacactgcat
      961 tcgctcggct ttatgcggga cgaaaaggct gacggcaagc gccatatgca gcaggcctgg
     1021 caccacatgc tcatggggct gtgcgcagtt ctcaagcagg atgccaggat acggccggat
     1081 gcctttgatg agcagttcac ggcagaaaaa tttgccgagg tgctgttttc acagctgttg
     1141 tccgccctgc tgcggcagga ttatgacccc agtgctgtgc tggaggtcgt gcgcagaacc
     1201 ttatattgac caccggcttt tcattcccac cagcgtgtgg gaattttaaa tgcttcaaac
     1261 tgaacaatgt tcacaatgcc tacaaaaatt atcagcaaaa aggagaaact gatctatgca
     1321 agcaatcgta gaaactgtat ttgatgccgt ctatctggta tctgtcatca ccatcggtat
     1381 cctgatgatc cgtgggagca agggtaaccc gcagttccgg ctgttcggcc tgatggccgt
     1441 tgtgctgggc gccggtgact ccttccatct gattccccgt gcgctggcgc tctgcaccac
     1501 gggtctggaa aactacaccg tgcccctggg cctcggcaag tggatcacct cggtcacgat
     1561 gacaattttt tatgtgctgc tctactatgt gtggcgtcag cgctacaggg tcgaggggcg
     1621 gaagagcctg accgctgccg tctacgggct ggctgccgtg cgcgtcatcc tttgcatgat
     1681 gccgcagaac cagtggctca gcaccgaatc cccactttcc tggggcatct accgcaacat
     1741 cccctttgcc ctgatgggcc ttctgattat cgtgctgttc ttccgcagcg ccagggagca
     1801 taacgaccgg gcgttccgct ggatgtggct gaccatcgtg ctgagctttg gcttctatat
     1861 cccggtcgtg ctgtgggccg atgcgatccc gatgatcggt atgctgatga tccccaagac
     1921 ctgcgcttat gtctggacgg tgctgatcgg ctattccgct atgaagaagg agtgcagata
     1981 agccacagca ccccgcccgc agaagcccgg cactccctga gaaaaaaatc cggcattttg
     2041 ccctgacccc cgatgctgcc ggcagggccg ggtttgccgc cttcctccgg gagtatgagc
     2101 gggcacgcaa ggcggcgcga gcggtagtct gatgtaagcg gcccttttga atcaatatcc
     2161 ctgcataaac acccccgccc ctgcgcatac tgtcagcacg gaggtgttta gctatgaact
     2221 atcttgactg tgcacagtat gattgctgcc ataacaaggc cgggtgctgc tgtttgaaca
     2281 gtatcaacgt ccgacacacc gggccggggg atgccgtctg cggcagctac cgcaacagcg
     2341 aggggtacgg caacgtcgcc accgacaacc agccggcctc gcccgagacg gatatccact
     2401 gcgacgacaa aggctgccgc cacctggaag gccgctgttg ctgtgccgac cacgtcagca
     2461 ttgacgagcg cggctgcgga ccggcctgcg ccacccgcgc cccgcgcaca gctgaataat
     2521 cgcccgcgca aaaacggatg ccgctttttc aaagtggtat ccgctttttt gttgcgaaat
     2581 acggcccatt gttgtataat aagacgatat agtaggtgtt gtccgccctt ttgcggggcg
     2641 ggacgccgca tgggcgggac gtcgggggcc gattcctgca aggaccgcct attttgagcg
     2701 gtgcgcagca ccgcgatcca ccctatttat tatctattat cttttagcga ggtgctcctt
     2761 atgtccgaaa ctcccatcac ggtgccgttt gtctccttcc gcccgatgga gcgggagctg
     2821 gatgccgagc tgcgcggcgc gtttgcccgc gtgctggaca acagctggta tatcggcggc
     2881 cgcgaggatg ccgcttttga aaaagccttt gccgaatact gcggcgtcaa gcactgcatc
     2941 ggctgcggca acggcctgga tgcccttgtg ctgattctga aagcctacgg catcgggacg
     3001 ggggacgagg tcattgtgcc gtccaacacc ttcattgcca cggtgctggc cgtcagctac
     3061 gcgggcgcga cgcccgtttt ggtcgagccg acgctggaaa gctacaacat cgaccccgcc
     3121 cgcatcgagg ccgccgtgac cccgcgcacg aaggccatca tggccgtgca cctctacggc
     3181 cagtgcgccc cgatggacga aatcaatgcc atcgccaagg cccacggcct caaggttatc
     3241 gaggatgccg cccaggccca cggggccgag tacaagggcc gtcgcgcggg cagtttgggc
     3301 gacgccgcag ggttcagctt ttacccgggc aaaaacctcg gagccctggg cgacgcgggc
     3361 tgcgtgacga cgaacgacga cgaactcgcc gagcgtgtcc gcgccctggg caactacggc
     3421 agcgactaca aataccacca tatttataag ggccaaaact cccgcctgga cgagctgcag
     3481 gccgcgttcc tggccgccaa gctgccccat ctcgacacta tgaacgccga gcgccgccgc
     3541 attgccgcgc ggtatctggc cgaaatgcac aacccggcgg tcacgctgcc cgccgagctg
     3601 cccggctgca cccacgtctg gcatatcttc gccgtgcgct gcgctgaccg caacgcgcta
     3661 gaaaagcatt tgaacgcccg cggcatcggc acgaacaagc actacccgac gcccattcat
     3721 ttgcaggggg cctacgccga gctgggcatc cccaagggcg cactgccgct ggccgaggaa
     3781 atctccgcca ccgagctgag cctgccgatg ttctacggta tgaccgacgc gcaggtgcag
     3841 gccgtcattg acgctgtgaa cgattttaag gggtgaacca tgccgaaagt cggtatcgta
     3901 atttccaact ataacggctg gcaggacacg ctggtctgcc tggaaagcct gcaaaaaatg
     3961 acgttccggg attttgaaat cgtcttaatt gacgatgctt cgcccaatga ctcggtggcg
     4021 cagttgcaag gcaaactgcc gccgaacacc gtgttcctgc cgcaggagaa aaacgtcggc
     4081 tttgcggcgg ccaacaatat cgggatgcgc cgtgccctgg ccgatggctg cgactatgcg
     4141 ctgctgctga acaacgacac atccgtccac ccggattttt tggaaaagct gctggccgag
     4201 acgccggaag gcgcggtcag ctgcccgaaa atgctgttta tggacccgcc cgacgagatc
     4261 tggtttgcgg gcggtgagct ggaccccgcc accggcaagg tcaagcacct gggcggccac
     4321 aaaaaggatg gccccgaatt tgccgagaaa cggcaggtca gcttcatcac tttctgctgt
     4381 gtgctgctgc cccgggcggt cattgaaaag gtcggctatc tcgatgagac gctgtttatg
     4441 tactgcgagg acgttgacta ctgcattcgt ctggcgcagg tcggcgtgcc gatgtggttc
     4501 ctgcccgacg ccgtcatcca ccacaaggcg ggcggcagtg cgggcgggat gctgtcggtc
     4561 tactacatca cgcgcaacac gctctacctg acctgcaagg gcaaaagtgc ggcgtacaaa
     4621 aagctgaaaa ccgttatccc catcctgacc ggcgcggcgc ggtacgccct gaccaaagcg
     4681 ctgggccgca aaaaaggccg cagctacggc gcgtacagag gtgccgtgga cttctggcaa
     4741 gggaagatgg ggagaatgga ggggtaagcg ggcggcccgg cgaatggcgg cggggtgagg
     4801 gcaccccgcc ctacaaccac ccgtagggcg gaatgcccgg cccgcagcat ccccgcaaac
     4861 gcaatggcaa ggcaaggccg agggccggat atgtccggcc cctacaaatt gactggaagg
     4921 gagacgtcct atatgccgca gttgaccatc gtcatcctgc aataccggcc agacgctgcg
     4981 gcgctgcggc gcacgctggc ctcactggca atgcaggata cccgcgattt cgccgttgtc
     5041 ctgggggacg acggttcaca acaggactat tttgccgaga gccgcgccta cctggcggct
     5101 cacggcatca ccgatgtgca gaccgccaag ctgatcccca acggcggcac cgtaaaaaac
     5161 gcccaaaacg ccctgcgtac cgccgccacc cgctgggtgc tcatgctctc gccgggagac
     5221 ttcctctatg acagtgagac ggtttcctgg tggctgggcc gtttgcaggc cgatggtccc
     5281 cgcgtggctt tcggcaagca ggcctacttc atccccggcc cggccccaca accgaccccg
     5341 ggggaaaccc cctttgaccg cacgccctac gaccccgccc actacgacgc caagaccatc
     5401 cgccggaacc tgctattgta cgacgatggg atcagcggcg cggggcttgt gtacgagcgg
     5461 acgcttttcc tctcggcact ggacaagatg gccgaccatg tgcggctggc cgaggatttc
     5521 tccctgcgac ttttcgctgt gcagggggta cgtatcaccc ggtacgaccg cctgaccgtc
     5581 tggtatgagt acggcggcgg cgtctcgacc gatgcagctg cccgggagcg gatgctgggg
     5641 gactggcgcg ccatgctgga agtgctgcgg gctgcatacc cccacgaccg caccgtgcgg
     5701 ctggcctacg
//
LOCUS       DBMDSQ010000109         5658 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_3526_length_5658_cov_2.009897, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000109 DBMDSQ010000000
VERSION     DBMDSQ010000109.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 5658)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5658)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5658
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3526_length_5658_cov_2.009897"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(265..2754)
                     /locus_tag="ACZPAX_05255"
     CDS             complement(265..2754)
                     /locus_tag="ACZPAX_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008716025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HIR1113340.1"
                     /translation="MINTIQIRGARVHNLKNIDVDVPLNKIVGIAGVSGSGKSSLALG
                     VLYAEGSRRYLDALSTYTRRRMTQAAKAQVDEVLYVPAALALHQRPGVPGIRSTFGTG
                     TELLNSLRLMFSRLASHRCPNGHYIPPTLNVAAEQPIYCPECGARVRAPSAEELAFNS
                     QGACRTCDGTGMVRTVDRATLVPDDSLTIDEGAVAPWNSLMWSLMTDVCRAMGVRTDV
                     PFRELTDKEKDIVFNGPAEKKHIFYKAKSTPEAGELDFTYFNAVYTVENALAKVKDEK
                     GMKRVEKFLRVDTCPDCGGSRLSEAARAPKLRGIGLDEACKMTLTALCDWVDGVPASL
                     PPEMRPMAESICESFRVGAKRLLDLGLGYLTLDRASATLSTGERQRMQLARAVRNRTT
                     GVLYVLDEPSIGLHPSNIVGLNGVMHDLIADGNSVVLVDHDTQVLAESDYIIEMGPEA
                     GAGGGRIIAQGTVPELTANAASQIGPFLGGQGSVTPQNRPAEELFAEGRIHLSTGTIH
                     TVKPLEVDIPKGRLTVVTGVSGSGKTTLILESLVPALAAKTSGAALPAHVKDVQAVGI
                     GQVKLIDATPIGINVRSTVATYANVHDELRKVFARTPDAKRLGYKAGDFSYNTGKLRC
                     PVCDGTGTISLDVQFLPDVEIPCTACRGSRYAPEADAVYHENKHGGAYTLPELMDMDI
                     NTALTACTDLKLVTQRLKVLQDLGLGYLTLGEETPSLSGGEAQRLKLASEMGKAQGDT
                     VFVFDEPTIGLHPLDVRTLLGVFRTLIENGATVIVIEHDLDVIRSADYIIDIGPEGGD
                     KGGTVVAQGTPEEVAQSPVSYTGKYVKKYLK"
     gene            complement(2799..3098)
                     /locus_tag="ACZPAX_05260"
     CDS             complement(2799..3098)
                     /locus_tag="ACZPAX_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005934408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HIR1113341.1"
                     /translation="MNNYIYIAVMFAVSYTIRILPLTLIRKPIKNQFIQSFLYYVPYV
                     TLAVMTFPAIIEATQTPISGAAALAAGIFAAWCGAGLFPVSVICCVLVFVIELFV"
     gene            complement(3091..3819)
                     /locus_tag="ACZPAX_05265"
     CDS             complement(3091..3819)
                     /locus_tag="ACZPAX_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004066087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HIR1113342.1"
                     /translation="MEQSTKFSRKVFCDGMRDGFPIALGYFAVAFSLGIAARNAGLTP
                     VQGFLASILNNASAGEYAAFTLIAAKATYWEVAVITLIANARYLLMSCALAQRFSPET
                     PFFHRLLIGYDVTDELFGITIARPGYLNPYYTYGAILLAAPAWAIGTALGIIAGNALP
                     LRVVSALSVALYGMFLAIIIPPARKNRIVAALVVISFVLSFACEYLPGISALSGGTRT
                     IILTVVISAAAAVLFPVKQEADHE"
     gene            3987..4682
                     /locus_tag="ACZPAX_05270"
     CDS             3987..4682
                     /locus_tag="ACZPAX_05270"
                     /inference="COORDINATES: protein motif:HMM:NF017655.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraX family protein"
                     /protein_id="HIR1113343.1"
                     /translation="MSRDAIKYIAMLTMLINHIANVFLPAGMWYTNLCLYIGYFTAVT
                     MCYFLVEGYGYTRSKRKYAGRLLGFAVLAQLPYQLAFPENGIAGFVQFNMLFTLLLCF
                     LVLLAQERIHDRVLRGAAIVLLICTSLFCDWALLAPVFTLLFIWARDSRPRKQIAFAA
                     AAVLYGGMAGLGSGSLAETVGCAVPILAAGFVILYLYNGQRARRGRSFSKWFFYTFYP
                     AHLLVLGLIRILA"
     gene            4837..>5658
                     /locus_tag="ACZPAX_05275"
     CDS             4837..>5658
                     /locus_tag="ACZPAX_05275"
                     /inference="COORDINATES: protein motif:HMM:NF015859.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE domain-containing protein"
                     /protein_id="HIR1113344.1"
                     /translation="MKKRNDQNVIVKRAVLVFILMFLVLLAALAFFTRSIVMREQLKA
                     SYTAEATVANVEAQLNRYLAESELLKSIVESGYEISDSDFAALSKLMYNEDGIIKAVE
                     LAPNGVVRSVYPKASNADVLGLNMLRHPERKVEASLAKESGQYTIAGPFQLVQGGTGA
                     LLFDPIYTTGADGSQKYWGFSVLVLDWESFLDEIQIDKLQDASYEYLIWKEGHNGERI
                     VIAQGDRPVGPNTLEVACAVPNDTWYFEIRPATGWITPTQEAFDVLLLLVLPAARS"
BASE COUNT         1119 a         1676 c         1660 g         1203 t
ORIGIN      
        1 cttgtcggca tggaaatccc ctttctgttc ttacgttctc ctcatcccct tcaactgatc
       61 attacttttt acagtctcta ccggggactt atccggttta gttataacaa ctccataaaa
      121 taatgcgatc aaaacagtcc aacagggctt cacatcctga taagccggat caaaatatat
      181 tatttacgag aattgctata aataatctct tctattatat acaacttcca tttaaaaaga
      241 aagtcctgca atatgatttc aagattattt cagatatttc tttacatatt ttccggtata
      301 ggaaaccggt gactgtgcca cctcctcagg agttccctga gcaactacgg tacctccttt
      361 gtctccaccc tccggtccga tatcaatgat ataatctgcg ctgcggatga catccaggtc
      421 gtgctcaatg acaatgaccg tcgcgccgtt ctcgatcagc gtgcggaaca cccccagcag
      481 ggtgcgcacg tccagcgggt gcaggccgat ggtcggctcg tcaaagacga acaccgtgtc
      541 gccctgggcc ttgcccatct cgctggccaa tttcagccgc tgggcctcgc cgccggaaag
      601 gctcggcgtt tcctcgccca gggtcaggta gccaaggccc aagtcttgca gcacttttaa
      661 acgctgtgtc accaatttta agtctgtgca agccgtcagc gccgtgttga tgtccatatc
      721 catcagctcg ggcagtgtgt aggccccgcc atgcttattc tcgtggtaga cggcgtcggc
      781 ctccggcgca tagcgagagc cgcggcaggc cgtgcagggg atctccacgt cgggcaaaaa
      841 ctgcacgtcc aggctgatgg tgcctgtgcc gtcgcagacc gggcagcgca gtttgcccgt
      901 gttgtaggag aaatcgcccg ccttgtaccc cagccgcttg gcgtccggcg tgcgggcaaa
      961 gaccttgcgc agctcgtcgt ggacattggc gtaggtcgcc acggtggagc ggacgttgat
     1021 gccgataggc gttgcatcaa tgagctttac ctgcccgata cccacggcct gcacatcttt
     1081 gacgtgggca ggcagggccg cgccgctggt tttggccgcc agcgccggga caaggctttc
     1141 caaaatcaac gttgtcttgc ccgacccgga cacgccggtc acgacggtca ggcggccttt
     1201 cgggatatct acttccagcg gcttgaccgt gtggatggtg ccggtggaaa ggtggatgcg
     1261 cccctcggca aacagttcct cggcggggcg gttttggggc gtcacgctcc cttgcccgcc
     1321 caaaaacggc ccgatctgtg acgcggcgtt ggcagtcagc tcgggcacgg tgccctgggc
     1381 aatgatacgc ccgccgcctg caccggcctc ggggcccatc tcgatgatgt agtccgattc
     1441 tgccaacacc tgggtgtcgt ggtcgacaag gacgaccgag ttgccgtcgg caatcaggtc
     1501 gtgcatgacg ccgttcaggc cgacgatgtt cgaggggtgc agcccgatgg atggctcatc
     1561 cagcacgtac agcacgccgg tggtgcggtt gcgaacggcg cgggccagct gcatgcgctg
     1621 gcgttccccg gtggagagcg tcgcggacgc gcggtccagc gtcaggtagc ccagccccaa
     1681 atccagcagc cgcttcgcgc cgacgcggaa ggactcgcag atgctctcgg ccatggggcg
     1741 catttcaggc ggcaggctgg cgggtacgcc gtccacccag tcgcacaggg cggtcagcgt
     1801 cattttgcag gcctcgtcca ggccgatgcc ccgcagtttt ggcgcacgag cggcctccga
     1861 caggcgcgaa ccgccgcagt cagggcaggt gtccacccgc aggaatttct ccacccgctt
     1921 catgcccttc tcgtccttga ctttggcgag ggcgttctca acggtgtaga cggcgttaaa
     1981 gtaggtaaag tccagctcgc ccgcctcggg ggtgcttttt gccttgtaga agatgtgctt
     2041 tttctcggcc ggaccgttaa aaacaatgtc tttctctttg tctgtcagtt cacggaacgg
     2101 tacgtctgtg cgcaccccca tggcgcggca gacatcggtc atcaggctcc acatcaggct
     2161 gttccacggt gcgacggccc cctcgtcgat ggtcagagaa tcgtcaggca caagggtggc
     2221 ccggtccacg gtgcgcacca tgccggtgcc atcacaggtg cggcaagcgc cctggctgtt
     2281 gaaggcaagt tcctctgccg acggcgcacg gacgcgtgcg ccgcactccg ggcagtaaat
     2341 cggctgctcg gcggcaacgt tcagcgtcgg cgggatgtaa tggccgttcg ggcagcggtg
     2401 gctcgccagg cgggagaaca tcaggcgcag actgttcaag agttccgttc cggtgccgaa
     2461 ggtgctgcgg atgccgggca caccgggccg ctggtgcagc gccagcgcgg caggcacgta
     2521 gagaacttca tcgacctggg ccttggcggc ctgggtcatg cggcggcggg tgtaggtgga
     2581 cagggcatcc aggtagcgtc gcgaaccctc ggcgtagagc acgcccagtg ccagcgacga
     2641 cttgcccgac ccggacacgc ccgcaatgcc gacgatttta ttcagcggca catcgacgtc
     2701 gatgtttttt aagttatgca cccgcgcccc gcggatttgg atagtgttta tcatgggatt
     2761 cctcgttctg caaattgtag gggcggccat tggccgccct acacgaatag ttcaatcaca
     2821 aacaccagta cacagcagat caccgatacc gggaacagcc ctgcgccgca ccaggcggcg
     2881 aagatgcctg ccgccagtgc cgccgcgccg gaaatgggcg tctgggtcgc ctcaatgatg
     2941 gccgggaagg tcatgaccgc cagcgtcaca tagggcacgt agtacaagaa cgactggatg
     3001 aattggtttt tgatgggctt gcggatcagc gtcagcggca aaatgcggat ggtgtaggac
     3061 accgcgaaca tgaccgctat ataaatgtag ttattcatgg tctgcctcct gcttgaccgg
     3121 gaacagcacc gcagctgccg ccgagatgac caccgttaaa ataatggtcc gcgtgccgcc
     3181 ggacaatgcc gagatgccgg gcagatactc acaggcgaag ctcagcacaa agctgatgac
     3241 caccagcgcc gccacaatgc ggtttttgcg ggccggcggg atgatgatag ccaaaaacat
     3301 accgtacagc gccacgctca gcgcactgac aacccgcagc ggcagcgcat tgcccgcgat
     3361 aatgcccagc gccgtgccga tggcccaggc gggcgcggcc agcaggatcg cgccgtaggt
     3421 gtagtagggg ttcaagtacc cgggccgcgc gatggtaatg ccgaacagct cgtcggtcac
     3481 atcatacccg atgagcagcc gatgaaaaaa cggcgtctcg ggcgagaacc gctgggccag
     3541 ggcgcagctc atcaagagat accgcgcgtt cgcgatcagc gtaatgaccg cgacctccca
     3601 ataggtcgcc ttagcggcga tcagggtgaa cgccgcatac tcccccgccg aagcgttatt
     3661 gaggatgctg gctaaaaagc cctgcaccgg ggtcagcccc gcattgcgtg ccgcaatgcc
     3721 cagcgagaac gcgacggcaa aataccccaa ggcaatgggg aacccgtctc gcataccgtc
     3781 acagaacacc ttgcggctga attttgtaga ttgttccata ttcctgtatc ttcctttcac
     3841 cgcgttttat tataacgcag atgcgcagga aaagaaagag ggcgggccgg atgtccgccg
     3901 cagaattttt atgagctttg caatattgcc cccaggatat atcgtaagta taatagatgt
     3961 gatacttgaa aaaaggagtt ctccccatgt cccgcgatgc catcaaatat attgccatgc
     4021 tcaccatgct tatcaaccac attgccaatg tgtttctgcc cgccgggatg tggtatacca
     4081 atttgtgcct ttacattggc tattttaccg ccgtgacaat gtgctatttc ctcgtcgagg
     4141 ggtacggtta cacccgctcc aaacgcaaat acgccgggcg gctgctgggg tttgctgtac
     4201 tggcacagct gccctaccag ctggctttcc cggaaaatgg cattgcgggc tttgtgcagt
     4261 tcaatatgct gttcaccctg ctgctctgct ttctggtgct gctggcgcag gaaagaatcc
     4321 acgaccgcgt gctgcgcggg gcggccattg tgttgttgat ttgcacgtcg ctcttctgcg
     4381 attgggcgct gctggccccg gtgttcacac tgctgtttat ttgggcgcgg gattcccgcc
     4441 cgcgcaagca gattgccttt gccgcggcgg cagtgctcta tggcggcatg gcaggccttg
     4501 gcagcggctc actggcggaa accgtcggct gcgcggtgcc catcctcgct gccgggttcg
     4561 tcatcctcta cctttacaat gggcagcgtg cccggcgggg gcgcagcttt tccaagtggt
     4621 tcttctatac gttttacccg gcacacctgc tggtgctggg gctgatccgc atccttgcgt
     4681 gaattttgcc gtatgctttg ccgcatacgg cttttttgtt cggttttgtt cagggttttg
     4741 ttcagatttt caggccctgc ttgcaacttg cccgccaaac aactataatg gttatatgta
     4801 actttgcata aaaatccccc acagggagtt gaagatatga aaaagagaaa cgaccagaat
     4861 gtcattgtaa aacgggcagt tctcgtcttt atcctcatgt ttcttgtgtt gctggcggcg
     4921 ttggcatttt tcacccgcag catcgtcatg cgcgaacagc tcaaggcgtc ctacacggcg
     4981 gaggccaccg tcgccaatgt ggaggcccag ctcaaccgct acctggcgga gtccgagctg
     5041 ctgaaaagca tcgttgagtc cgggtatgaa atcagtgaca gcgattttgc cgcactgtcc
     5101 aagcttatgt acaacgagga cggcattatc aaggccgtag agctggcccc gaacggcgtc
     5161 gtgcgcagcg tttacccgaa ggcgagcaac gccgacgtcc tggggctgaa tatgctccgg
     5221 caccccgagc gcaaagttga agccagcctt gccaaggaga gcggccagta cacgattgcc
     5281 gggccgtttc agctggtgca gggcggcacc ggcgcactgc tgttcgatcc tatctacacc
     5341 accggcgcgg acggcagcca aaaatactgg ggcttctccg tcctggtgct ggactgggag
     5401 agctttctcg atgaaatcca aatcgacaaa ttacaggatg cctcctacga gtacctgatt
     5461 tggaaggagg gccacaacgg tgagcggatc gtcattgccc agggcgaccg ccccgtggga
     5521 ccgaacacac tggaagttgc ctgcgccgtg cccaacgata cctggtattt tgagatacgc
     5581 ccggccacgg gctggatcac cccgacccag gaagcctttg atgtgctttt gctgctggtg
     5641 ctgcccgcgg cccgcagc
//
LOCUS       DBMDSQ010000110         5603 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_3559_length_5603_cov_1.697565, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000110 DBMDSQ010000000
VERSION     DBMDSQ010000110.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 5603)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5603)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5603
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3559_length_5603_cov_1.697565"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(143..2302)
                     /locus_tag="ACZPAX_05280"
     CDS             complement(143..2302)
                     /locus_tag="ACZPAX_05280"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005933140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HIR1113345.1"
                     /translation="MAYEFASRLETFPKYRKFETTFAGRPFVVETGKMCGLSNGSAMI
                     RYGETCVLCNVTMSDKPRDGVDFFPLSVEFEEKLYAAGRIPGSFMRREGRPGEHAILS
                     SRVVDRPIRPLFPKDMRNDVCVTMTVMSLDPDCSAEISGMNGASLAIAMSDIPWGGPI
                     AGVFVGLVDGEIVLNPTKEQREHSDLSLTLAASEEKIVMIEAGANEVDEETMMKAIRA
                     GHEEIKKMLAFINGIVAEIGKPKKSFTPVELDHALLADVYAKHLDEVKAAMDTDDKNV
                     RDAALLPIMDAIAAEHPDLTAADLDLISYKLQKKVVRTWLLEDGKRVDGRGINEIRPL
                     AAEVGLLPRVHGSGMFTRGQTQVLTICTLGSTKDAQLMDDLSDTPYKRYIHHYNFPPY
                     SVGEARAPRSPGRREIGHGNLAERALIPVLPDQAEFPYTIRCVSEVLSSNGSTSQASI
                     CGSTLALMDAGVPIKAPVAGISCGLITDGDPLHGGRWMTMLDIQGVEDFHGDMDFKVG
                     GTRRGITAIQMDIKVDGLTYEIVEEALEKCRKGRLYILDQIIKPVIAEPRAELSKYAP
                     KMFSMMIPVDKIKDVIGKGGKVIQEMCANFNCKIDIEEDGHVFISAVDQDDAKRAIAT
                     IKTIVEDPEIGAIYKGRVTRLMNFGAFVEIAPGKEGLVHISKLDDHRVEHVEDVVAVG
                     DPIFVMVTDIDQQGRINLSRKDALAAIAKKRAEAAQQ"
     gene            complement(2605..2877)
                     /gene="rpsO"
                     /locus_tag="ACZPAX_05285"
     CDS             complement(2605..2877)
                     /gene="rpsO"
                     /locus_tag="ACZPAX_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008688610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HIR1113346.1"
                     /translation="MSQKSAIDKQPIIAKAAIHEGDTGSPEVQVALLTARINHLTEHL
                     KTNKHDNHSRRGLFKMVGRRRNLLAYLQKKDINRYRALIAELGLRK"
     gene            complement(3033..4616)
                     /locus_tag="ACZPAX_05290"
     CDS             complement(3033..4616)
                     /locus_tag="ACZPAX_05290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113347.1"
                     /translation="MKNLSRKETCWAILQQVPTYLIAVLSLLICGFLTVASLFSLAVM
                     RMDEIPVLGELPEQLVLADRNVLHVAPVVAVLSILLLLLARQNFTWKHAKIVTWVAAA
                     VQTILGFWWKSSFASSPVADQGTFWNVAESLAGHQAPSAELLDYLRYWPFQAAVGMTG
                     EPFARLFNGNYGAWRVMNAFCVGGCVMLLSCICGRITNSPRAKACCAVLVAGFFPLNM
                     YTTFVYGTLSGTMTSLLAVYAVIRQCTAEDKKSERKWWAVGTVAITLAITLYTGMQIF
                     LVAVTLLLLATGIFHRDQRQKILGAVLMFVVAFGFQHGWQALAMHRMGLGNEPGCPIW
                     PRVAMGVDANTDVTPGFYNCVNAGLYYASNFVPKAANERAMDYIVLCLNQLWETGRFG
                     SFFYEKTADQWLEPWFGGLTMNNPSIFEEPKWLATAITSKTLFVPVHAWLSMLISVVY
                     VWSTVGVVVLLRKHKTQVWQLSLAVCLIGGFVFQLAAEAKARYCLPYFLCCFPLAAAG
                     LAAAAQKLPCQKRAENTAK"
     gene            complement(4642..>5603)
                     /gene="ribF"
                     /locus_tag="ACZPAX_05295"
     CDS             complement(4642..>5603)
                     /gene="ribF"
                     /locus_tag="ACZPAX_05295"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HIR1113348.1"
                     /translation="KSKNYFAKGDDRPMQIYQSLTPVRAPQGSAVALGYFDGVHCGHR
                     AVLGAAVDCAKANGLTAAAFTFELPAGSTLKGGRILSLRQKHARVAALGIEEYQEAPF
                     DEFRALSPEDFVQTVLVECFNAKELFCGDNFTFGARAAGNVERLRELCKPLGIGVHIV
                     PMAQYQGQTVSSTRIRAALEEGRLADANAMLGTPYAIDWPVVHGKGIGSGKLGTPTLN
                     QNYPAAALQPCAGVYLTRIFLNGTWWPAATGIGKRPTVDQSADAAVTCETFVPDFTGN
                     VYGQQPVLEFYKYFCPVRKFNSLIELAALIHHAADESKAYFAE"
BASE COUNT         1128 a         1627 c         1739 g         1109 t
ORIGIN      
        1 cggctatgct gcgggccggg catgcccggc ccctacggca caataaaaat tcaaaaaggg
       61 aggctgccca cctgggcagc ctccctttta cggcttattt gtttggagtt cttttggtta
      121 cttttcttgc aagaaaagta acttactgct gagcggcctc ggcgcgcttc ttggcgatgg
      181 ctgccagggc gtccttgcgg gacagattga tgcggccctg ctggtcgatg tcggtcacca
      241 tgacaaagat gggatcaccg acggcaacga catcctcaac gtgctcaacg cggtggtcgt
      301 ccagcttgga gatgtgaacc agaccctcct tgccgggggc aatctcaaca aaggcaccga
      361 agttcatcag gcgggtcacg cggcccttgt agatggcacc gatctcggga tcctcgacaa
      421 tggtcttgat ggtcgcaata gcgcgcttgg catcgtcctg atcgacagca gagataaaga
      481 catggccgtc ctcctcaatg tcgatcttgc agttgaagtt ggcgcacatt tcctggatga
      541 ccttgccgcc cttgccgatg acatccttga tcttgtcgac cggaatcatc atgctgaaca
      601 ttttgggcgc atacttggac agctcggcgc gcggctcggc aatgaccggc ttgatgatct
      661 ggtcaaggat gtacaggcgg cccttgcggc acttttccag cgcttcctca acgatctcat
      721 aggtcaggcc gtccaccttg atgtccatct ggatggcggt gatgccgcgg cgggtgccgc
      781 cgaccttaaa gtccatatcg ccgtggaagt cctcaacgcc ctgaatgtcg agcatcgtca
      841 tccagcggcc gccgtgcagc gggtcaccat cggtgataag gccgcaggag ataccggcaa
      901 cgggtgcctt gatgggcaca ccggcgtcca tcagggccag cgtagagccg cagatggaag
      961 cctgggaggt ggagccattg gaggacagaa cctcggagac gcagcggatg gtgtagggga
     1021 actcggcctg atcgggcagt acggggatca gggcacgctc ggccaggttg ccatggccga
     1081 tctcgcggcg gccggggcta cggggtgcgc gagcctcgcc gacggagtac ggcgggaagt
     1141 tgtagtggtg gatatagcgc ttgtaaggcg tgtcggacag gtcatccatc agctgggcat
     1201 ccttggtgga gccgagggtg cagatggtca gcacctgcgt ctggccgcgg gtgaacatgc
     1261 cggagccgtg gacgcggggc agcaggccaa cctcggcggc cagcgggcgg atctcattga
     1321 tgccgcggcc gtccacgcgc ttgccgtcct caagcagcca ggtacgaacg accttcttct
     1381 gcagcttgta gctgatgagg tcgaggtcgg cggcggtcag gtcggggtgc tcggcggcaa
     1441 tagcatccat gatgggcagc agggcggcgt cgcggacatt cttgtcgtcg gtgtccatgg
     1501 cagccttgac ttcgtccaga tgcttggcat agacatcggc cagcagggca tgatccaact
     1561 ccacaggggt aaagctcttc ttcggcttgc cgatctcggc gacgataccg ttgatgaagg
     1621 cgagcatctt cttgatttcc tcatgaccgg cgcggatggc cttcatcatg gtctcctcgt
     1681 cgacctcgtt ggcaccggcc tcaatcatga cgatcttttc ctcgctggcg gccagggtca
     1741 aggacaggtc actgtgctcg cgctgctcct tggtggggtt cagcacgatc tcaccatcca
     1801 ccagaccgac aaagacaccg gcgatggggc cgccccacgg aatatcagac atggcgatgg
     1861 ccagggacgc gccattcatg ccggagattt cggcagagca gtcgggatcc aggctcatga
     1921 cggtcatcgt cacgcagaca tcgttgcgca tatccttcgg gaacagcggg cggatggggc
     1981 ggtcaacgac gcgggaggac aggatggcgt gctcgccggg gcggccctca cggcgcatga
     2041 agctgccggg gatgcggccg gcggcgtaga gtttttcctc aaactcgacg ctcaggggga
     2101 agaagtcaac accgtcgcgc ggcttgtcgc tcatcgtaac gttgcacagc acgcaggtct
     2161 cgccgtagcg gatcatcgcg ctgccgttgg acagaccgca cattttgccg gtctcgacca
     2221 caaaggggcg gcccgcaaag gtcgtctcga acttgcggta cttggggaag gtttccagtc
     2281 tggatgcgaa ttcgtaagcc atagggattc tccttcttta gtagattttt actttcagcg
     2341 ctcccctgcc ccgattctgt acaataaagc cagcagccaa gccagttgct tgggtgctcg
     2401 atttactgca caaaatggcg gcggggcggc tgtaaaatac aggtgcagtg ggttttcagg
     2461 tgtgcattgc cggaaacggg cacgggcgga tatgaaatcc gcccacgggg cggtcgagga
     2521 cgccgcccct acaattacgg caaactgcgc acgtataaaa aaatcaggca gagtgcaaat
     2581 gcaccctgcc cgtttttgcg aaaattactt acgcagaccc agctcagcga tcagggcacg
     2641 gtaacggttg atgtccttct tctgcagata ggccagcaga ttgcggcggc ggccgaccat
     2701 cttgaacagg ccgcggcggc tgtggttgtc atgcttgttg gtcttaaggt gctcggtcag
     2761 gtggttgata cgagcggtca gcagagcaac ctggacctcc ggagaaccgg tgtcaccctc
     2821 gtggatggct gccttagcga tgattggctg cttatcgata gcggatttct ggctcatggg
     2881 taaatacctc tttctaaaaa atatattggc cgtcggcgca gtaaggggta gttggaaccg
     2941 ttgaggcata cgccccatac cgggccgcgg taacattagt agtatagcac aaccttttgg
     3001 ggaatacaag tgcaaatctg ctttttccgc aattattttg cggtgttttc cgcgcgtttc
     3061 tggcagggca gcttttgcgc tgcggcagcc agaccggccg ccgccagcgg gaagcagcac
     3121 aagaaatacg gcaggcagta ccgggccttt gcctcggcgg ccagctggaa cacaaagccg
     3181 ccgatcaggc agacggccaa gctgagctgc cacacctgcg ttttatgctt gcgcagcagc
     3241 acaacaacgc ctacggtgct ccacacatag accactgaga tcagcatact gagccaggcg
     3301 tgcaccggca caaacagtgt tttgcttgtg atggccgttg ccagccactt gggttcctca
     3361 aaaatgctgg ggttgttcat cgtcaggccg ccgaaccagg gttccagcca ctggtctgcg
     3421 gtcttttcgt agaagaagga gccgaagcgc ccggtctccc aaagctggtt caggcagagc
     3481 acaatgtaat ccatcgcgcg ctcgttggcg gccttgggta caaaattgct ggcgtagtac
     3541 aggcccgcgt tcacgcagtt gtaaaagccc ggcgtcacat cggtgttggc atccaccccc
     3601 atggcgacgc gcggccaaat tgggcagccc ggttcattgc ccaggcccat gcggtgcata
     3661 gccagggcct gccagccgtg ctggaaaccg aatgccacca caaacatcag cacagcgccc
     3721 aaaattttct gccgctggtc cctgtggaaa ataccggtcg ccaacaaaag cagcgtgacc
     3781 gccacgagga aaatctgcat acctgtgtac aatgtaatag ccagcgtaat ggcgactgtg
     3841 cccacagccc accacttgcg ctcggacttt ttatcctcgg ccgtacactg gcggatgacc
     3901 gcatagacag caagcagcga cgtcatggtg ccggacagcg taccgtacac aaaggtcgtg
     3961 tacatattga gtgggaagaa gcccgcgacc agcaccgcgc agcaggcttt ggcgcggggc
     4021 gagttggtga tacgcccgca gatgcaggag agcagcatca cgcagccacc gacacagaaa
     4081 gcgttcatga cccgccaggc accatagttg ccgttaaaca gccttgcgaa cggctcaccg
     4141 gtcatgccga cagctgcctg gaacggccag tagcgcaggt agtccagcaa ttccgcgctg
     4201 ggcgcctggt gcccggccag ggattccgcc acattccaga aggtgccctg gtccgcgacc
     4261 gggctgctgg caaacgaact tttccaccag aagcccaaga ttgtctgcac ggcggcggcc
     4321 acccaggtga cgattttggc gtgtttccat gtaaagttct gccgtgccag cagcaggagc
     4381 agaatgctga gcaccgcgac taccggggca acgtgcaaaa cgttacggtc ggcaagcacg
     4441 agttgttccg gcaactcgcc cagaacgggg atctcgtcca tccgcatcac tgccagggaa
     4501 aacagcgacg cgaccgtcag aaagccgcag atcaacaggc tcaaaacggc aatcagatag
     4561 gtgggcacct gctgcaggat ggcccagcag gtttcttttc gggagaggtt tttcatagtc
     4621 cggcgtctcc tcttttcttt ttcattccgc aaaatatgct ttactctcgt cggcggcgtg
     4681 gtggatgagg gccgccagct cgatgaggga attgaacttg cgcacagggc agaaatattt
     4741 gtaaaattcc agtaccggct gctggccgta gacattgccg gtgaagtccg gcacaaaggt
     4801 ctcgcaggtc acggcggcgt cggcgctctg atccacagtg gggcgcttgc cgatgccggt
     4861 tgcggccggc caccacgtac cattgaggaa aatgcgggtc aggtagacgc ccgcgcaggg
     4921 ctgcaatgcg gcggcgggat agttctggtt cagcgtcggc gtgcccagct tgccgctgcc
     4981 gatgcccttg ccgtggacga ccggccaatc aatggcgtaa ggcgtgccga gcatggcgtt
     5041 ggcgtcggcc aggcggcctt cctccagcgc cgcgcggatg cgggtggagg acacagtctg
     5101 gccctgatac tgggccatcg gcacgatatg gacgccgatg cccaagggtt tgcacagttc
     5161 ccgcagccgt tcgacattgc ccgccgcgcg ggccccgaag gtgaagttat cgccgcagaa
     5221 cagttccttg gcgttgaagc actccaccag caccgtctgg acaaaatcct cgggcgagag
     5281 ggcgcggaac tcgtcaaagg gggcttcctg gtattcctca atgcccaggg ccgccacgcg
     5341 ggcgtgcttc tggcgcagag acaggatgcg cccgcctttc agcgtgctgc ccgcaggcag
     5401 ttcaaacgtg aaagctgcgg cggtcaggcc gttggctttg gcgcagtcaa ccgccgcacc
     5461 cagcacggcg cggtggccgc agtggacacc gtcaaagtag cccagcgcca ccgcgctgcc
     5521 ctggggtgcg cggacgggcg tcaggctctg gtatatctgc attgggcggt catccccttt
     5581 cgcaaaatag tttttcgact ttc
//
LOCUS       DBMDSQ010000111         5498 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_3641_length_5498_cov_1.733185, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000111 DBMDSQ010000000
VERSION     DBMDSQ010000111.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 5498)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5498)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5498
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3641_length_5498_cov_1.733185"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..862)
                     /locus_tag="ACZPAX_05300"
     CDS             complement(<1..862)
                     /locus_tag="ACZPAX_05300"
                     /inference="COORDINATES: protein motif:HMM:NF015000.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HIR1113349.1"
                     /translation="MKILFCIVLILVLLVLFAVWPNKRRDALRAPFVGHNCAHRGYFG
                     KDQRPPENSLPAFAAAAQNGYGIELDVQFTSDRRLVVFHDDTLDRMTPAKGFVHDMDW
                     ANFSAQPLAGSNEHPPLFADMLRTVAETNPDTPLIVEIKSRAEYSKTYLEELCRATIA
                     ELKAYPGPYCIESFDPRVVGIVRRQAPDILRGQLADSYQSYRKDGTHAVPAFAFSHLF
                     GNFLGRPDFIAWCPAKRNWAVKLNAALGAMMVMWTALPEHDTAKLEAENDAVIFQWYG
                     PDAQSQLPGAG"
     gene            complement(930..1088)
                     /locus_tag="ACZPAX_05305"
     CDS             complement(930..1088)
                     /locus_tag="ACZPAX_05305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113350.1"
                     /translation="MQAYPAGRFPVFAGHNYMQYQIRDPRGFAAMLDSIIQYDTMPDL
                     PMVRREKS"
     gene            1283..1966
                     /locus_tag="ACZPAX_05310"
     CDS             1283..1966
                     /locus_tag="ACZPAX_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR1113351.1"
                     /translation="MAKILIVDDEPRIRDLIREHLQYAGFTCEEAGDGTAALSALSQG
                     GIDLVILDIMMPFMDGMTCLREMRTRKIMTPVIMLTARSEEYDKLAGLESGADDYVVK
                     PFSPRELVARVKAVLARTMPKPAEDSGSYTFGDLHIDTASHSVKVAGQEAPLTPKEFD
                     LLVFLVSNKGIALSREKILQKVWNYDYYGEDRTVDTHVKMLRSHLGPCRKYITTVWGI
                     GYKFDPDTL"
     gene            1963..3471
                     /locus_tag="ACZPAX_05315"
     CDS             1963..3471
                     /locus_tag="ACZPAX_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015565986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR1113352.1"
                     /translation="MRKPNTPKKPFALSIRWQLMLFIGGITLFTLLLVWGIITYALAP
                     QYNRNIRNKLDAKAAAITALIDQSDSAISNRSYLGLTLNEDFWDGVSETFQDKKINVD
                     SCCVDFSDTTLRCLKAYESMYPCLLHESTGAFDGAFGYNVDTHVAIQLRQKLFQNGEL
                     YQIVKVGSTMQMCVGRLSADGQYGVIVSTSLAQITTAAEVLRSILGPVALLLLVLDLL
                     FAMLFSRWFTNPIRKLSNGAQEIAAGNYDVAIKVEHHDDIGRLAEDFNYMAAEVKRSA
                     QLEKDILANVSHDLRTPLTLIKGYAETVRDLTGSDDAKRTEQCNIIVDETDRLSTLVN
                     SVMELSKVQSGAEKPNLVDFDMSELCFEVAGRYDALCDKNHWKLDLQADDACPVSADP
                     AMMERVLHNLLGNAFHHIGADGVVVLRAVPQEKGCRIEIEDHGPGIPPEDLPYIFDRY
                     YRARQDSGRTGTGLGLSITKAILQQHGFAFGVNSAVGKGSTFWFEMVDTRNK"
     gene            3597..4133
                     /locus_tag="ACZPAX_05320"
     CDS             3597..4133
                     /locus_tag="ACZPAX_05320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113353.1"
                     /translation="MENVNAKMDEQLKARVDDLTRSNDENRDGKLDAADLKAQLNRIE
                     AQLELQDRQNRGIIANQRKRMILSVVLVVVILAALAFFGWRMNIAYNNVMDACSRVND
                     LADTVNTSLATLDQSELDAMMQDLPEITDQLKKIDVDALNNALTQLPALMDTVNSLQS
                     QVQSIVNLFSGLSSVFGR"
     gene            complement(4343..4837)
                     /locus_tag="ACZPAX_05325"
     CDS             complement(4343..4837)
                     /locus_tag="ACZPAX_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015537153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HIR1113354.1"
                     /translation="MELTVRAATIAELPELMQLYTNARAFMQTHGNPTQWHAGYPGEA
                     RLAAEIERGVCHVVMGEGAIQAVFCLIPGDDPTYHHIEDGAWPEDVPYATIHRLASAG
                     HVGGIGRLCVQWCLQQGLPVRADTHADNTYMQHTLEACGFVRCGTIYTEDGTPRWAYY
                     HYAI"
     gene            5017..>5498
                     /locus_tag="ACZPAX_05330"
     CDS             5017..>5498
                     /locus_tag="ACZPAX_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015519198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HIR1113355.1"
                     /translation="MTLAELKVGQDAVLRTIGGQGELRHHLLDMGLTPGTEVTLRKVA
                     PMGDPIEVELRGYELTLRLADAAKIEVENIHETDRAARSEQRHTPVPHPGVGELRKAP
                     SYHDRKNGSEIAKGRPLRFALAGNQNCGKTTLFNQLTGSNQHVGNFPGVTVGFSAMQT
                     "
BASE COUNT         1189 a         1647 c         1602 g         1060 t
ORIGIN      
        1 atccagcacc aggaagttgg gattgcgcat caggaccgta ccactggaag ataacggcgt
       61 cgttctccgc ttccagcttg gcggtgtcat gctcgggcag ggccgtccac atgaccatca
      121 tagcgcccaa ggctgcattg agcttcacgg cccagttgcg cttggcaggg caccaggcga
      181 tgaaatccgg ccgccccaga aagttgccga acaggtggga gaacgcaaac gccggaacgg
      241 cgtgcgtccc atccttgcgg tagctctggt agctgtcggc cagctggccg cgcaggatat
      301 ccggggcttg ccgccgcaca atgcccacga cgcgggggtc aaagctttcg atgcagtagg
      361 gaccggggta ggctttcagc tcggcgatcg tggcgcggca aagctcttcc aggtaggtct
      421 tgctgtactc ggcgcggctc ttgatctcca cgatcaacgg cgtgtcgggg tttgtttcgg
      481 ccacagtgcg cagcatatcg gcaaacagcg gcgggtgctc gttgctgccc gccagcggct
      541 gtgccgagaa gttagcccaa tccatatcat gtacgaagcc tttggccggg gtcatgcggt
      601 ccagcgtgtc atcgtggaac accaccagcc gccggtccga cgtaaactgc acatccagct
      661 cgatgccgta gccattctgc gcggcggcgg caaaggcggg caggctgttc tccggcgggc
      721 gctgatcctt gccaaagtag ccgcggtggg cacagttatg ccccacaaac ggcgcacgca
      781 gggcgtccct gcgcttattc ggccagacgg cgaacagcac cagcagcacc aaaatcaaca
      841 caatgcaaaa caggattttc ataagtagtc actctcctgc cgtcattgta ccgcgccgcg
      901 cggcaaaatg caaccgccgt ttgcagattt tacgattttt cgcgccgcac catcggcagg
      961 tccggcatgg tgtcatattg tataatgctg tccagcatgg ccgcaaagcc ccgcgggtcg
     1021 cgtatctggt actgcatata gttatgcccc gcaaacaccg ggaaccgccc ggccggataa
     1081 gcctgcatga cgacggcacg gcatttgtag tgattctcgg catcgccaaa ggcaaaccag
     1141 caccgcgcct gcctttcggg atttcatatt cctattctat cacacttttg taaatttttt
     1201 tcgatttttt cacaattctt ccactcttgc gttgtattgt ggtaaaatag aaatggagaa
     1261 agcagcaagg aggtcttttc ccatggcaaa aattctgatt gttgacgatg agccgcgtat
     1321 ccgtgacctc atccgtgagc atctgcaata tgccggtttc acctgcgagg aagccggtga
     1381 cggtaccgct gccctcagtg cgttgagcca aggcggcatt gacctggtga ttttggacat
     1441 tatgatgccg tttatggacg gcatgacctg cctgcgggag atgcgcaccc gcaagatcat
     1501 gaccccggtc atcatgctga ccgcccgcag tgaggagtac gacaagctgg ccggcctgga
     1561 aagcggtgcc gacgattacg ttgtcaagcc cttttccccg cgggagctgg tggcccgcgt
     1621 caaggccgtg ctggcccgca cgatgcccaa accggccgag gactccggca gctacacctt
     1681 tggcgacctg cacatcgaca cggccagcca cagcgtgaaa gtggccgggc aggaggcccc
     1741 gctgaccccc aaggagtttg acctgctggt gttcctcgtc agcaacaagg gcattgccct
     1801 gagccgcgag aagattttgc aaaaggtctg gaactacgac tattacggcg aggaccgcac
     1861 cgtggacacc cacgtcaaaa tgctgcgcag ccaccttggc ccctgccgca aatacatcac
     1921 caccgtatgg ggcatcgggt acaaattcga cccggatacg ctatgagaaa gcccaacaca
     1981 cctaaaaagc cctttgcatt aagcatccgc tggcagctga tgctgtttat cggcggcatt
     2041 acgctgttta cgctgctgct ggtgtggggc atcatcacct acgcgctggc accgcagtac
     2101 aaccgtaata tccgcaataa gctggacgcc aaagcggcgg ccatcacggc gctgatcgac
     2161 cagtcggaca gcgcgatctc caaccgaagc tatctgggcc tgacgctgaa cgaggatttc
     2221 tgggacggcg tcagcgagac attccaggac aaaaaaatca atgtggacag ctgctgcgtt
     2281 gatttttccg acacaacgct gcgctgcctg aaagcctacg agagtatgta cccctgcctg
     2341 ctgcacgaga gcaccggtgc attcgacggc gcgttcggct acaatgtgga cacccatgta
     2401 gccattcagc tgcggcaaaa gctgttccag aacggtgagc tgtaccagat cgtgaaggtc
     2461 ggttccacca tgcagatgtg cgtggggcgg ctctcggcag acggccagta cggcgtcatc
     2521 gtctccacaa gtctggcaca gatcaccacc gccgccgagg tgctgcggtc gatcctgggc
     2581 cctgtggcgc tgcttctgct ggtgcttgac ctgctgtttg ccatgctgtt cagccgttgg
     2641 ttcaccaacc ccatccgcaa gctgtccaac ggcgcacagg agatcgcagc gggcaactat
     2701 gacgtcgcca tcaaggtgga gcaccacgac gacatcggac gtctggccga ggatttcaac
     2761 tatatggccg ccgaggtcaa gcgcagcgcc cagctggaaa aggacattct cgccaacgtc
     2821 agccacgacc tgcgcacgcc gctgaccctg ataaagggtt acgccgagac ggtgcgcgac
     2881 ctgaccggca gtgacgatgc caagcgcact gagcaatgca acattattgt cgatgagacg
     2941 gatcgcctct cgacgctggt aaacagcgtg atggaactga gcaaagtgca gagcggcgcg
     3001 gaaaagccaa accttgttga ctttgacatg agcgagcttt gctttgaagt ggcgggccgc
     3061 tacgatgcac tgtgcgacaa aaatcactgg aagctcgact tgcaggccga cgacgcctgc
     3121 ccggtgagcg ctgaccccgc tatgatggag cgggtgctgc acaacctgct gggcaatgcg
     3181 ttccaccaca tcggcgcgga cggcgtcgtg gtactgcggg ccgtcccgca ggaaaagggc
     3241 tgccgcattg aaattgagga ccatggcccc ggcatcccgc cagaggattt accctacatt
     3301 tttgaccgct actaccgcgc ccggcaggac agcggccgca ccggcaccgg cctgggcctg
     3361 tccatcacca aagccatcct gcagcagcac ggcttcgcct tcggtgtcaa cagcgccgtc
     3421 gggaaaggat caacgttctg gttcgagatg gtggatacga gaaataaata actgccagcc
     3481 gtaagggtcg gatatgtccg gccctcagct tacccgtaag cagcatctgc cgatacagcc
     3541 gcgggccggg cacgaccggc ccccacacca cagcacccca aaaaggaggc ccccacatgg
     3601 aaaacgtaaa cgccaaaatg gatgagcagc tgaaagcccg cgtggacgat ttgacgcgct
     3661 ccaacgatga aaaccgtgac ggcaagctgg atgccgccga cctgaaagcc cagctgaacc
     3721 gcattgaggc ccagctggag ctgcaagacc ggcagaaccg cggcattatc gccaaccagc
     3781 gcaaacggat gattttaagc gtcgtgctgg tggtcgtgat tctggcggcg ctggcgtttt
     3841 tcggctggcg gatgaatatt gcctacaaca acgtcatgga cgcctgcagc cgcgtcaacg
     3901 acctggccga cacggtgaac acgagccttg ccacgctgga ccagagcgag ctggacgcca
     3961 tgatgcagga ccttccggaa atcaccgacc agttgaaaaa aatcgacgtg gacgcgctga
     4021 acaacgccct gacccagcta cccgccctga tggacaccgt gaacagtctg caatcccagg
     4081 tgcagagcat cgtgaatctg ttcagcgggt taagttccgt gtttggacgg taaataaaat
     4141 gtagcgaaaa agccttcccc ctttggggga aggtggccgc aggccggatg aggggggcct
     4201 tgccgtaagt ttcgctttgt agggtggcat ggcaaacttg cccctcatca gcccgctttg
     4261 cgggcagctt ccccccaggg ggaagccttt tagcacagca acgccccgca ttccctatac
     4321 cgggaatgcg gggcgctgtt gtttatattg cgtaatggta gtacgcccac cttggtgtgc
     4381 cgtcctcggt gtaaattgtc ccgcagcgca caaagccgca ggcttccagc gtgtgctgca
     4441 tataggtgtt gtcggcgtgg gtatcggcgc gtaccggcag gccctgctgt aaacaccact
     4501 gcacgcacaa gcgcccgatg ccgcccacgt gccccgccga cgccagacga tggatcgtcg
     4561 cgtagggtac atcctcgggc caggcaccgt cctcgatgtg atggtaggtg gggtcgtcgc
     4621 cgggtatcag gcagaacacc gcctgaatgg ccccctcccc catcaccaca tggcagacgc
     4681 ctcgctcgat ttcagcggcc aggcgggctt caccggggta gcctgcgtgc cattgggtgg
     4741 ggttgccgtg ggtctgcatg aaggcccggg cgtttgtgta taactgcatc agttccggca
     4801 actcggcgat tgttgccgcg cggacggtca attccataga cagcactcct taataatacc
     4861 cgcaaagtat atcacatttc atcccggtgc acaatcactg aaactgcggc ggcttaccgc
     4921 ttttttgata tttttcttgc atccggcccc tgtgtgtgct acactgtatc tgcaaagtta
     4981 gaagagccaa acttttagac aaaagaggat tgtaaaatga cactggcaga actaaaagtt
     5041 gggcaggatg ctgtgctgcg cacgattggc gggcagggcg aactgcgtca tcacctgttg
     5101 gatatggggc tgacccccgg caccgaagtc accttgcgca aggtcgcacc gatgggcgac
     5161 cctatcgagg tggagctgcg tgggtatgag ctgaccctgc gcctggcaga tgccgcgaaa
     5221 attgaagtgg aaaatatcca cgaaacggat cgcgccgcac gcagtgagca gcggcacacg
     5281 cccgtccccc acccaggcgt aggcgaactg cgcaaggccc ccagctatca tgaccgcaag
     5341 aatggttcgg agattgccaa gggccgcccg ctgcgctttg ccctggccgg caaccagaac
     5401 tgcggtaaaa cgacactttt caaccagctt accggctcca accagcacgt cggcaacttt
     5461 cccggtgtga cggtgggctt ttcggctatg caaactat
//
LOCUS       DBMDSQ010000112         5430 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_3686_length_5430_cov_1.841434, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000112 DBMDSQ010000000
VERSION     DBMDSQ010000112.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 5430)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5430)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5430
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3686_length_5430_cov_1.841434"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            150..2663
                     /locus_tag="ACZPAX_05335"
     CDS             150..2663
                     /locus_tag="ACZPAX_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008118790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR1113356.1"
                     /translation="MEKLAAFIVDKRNLFFLLYIFALIFCAIATGWVKVEDDITTYLP
                     DTTETRQGLTVMNDNFVTYGTANVMVSNITYDTALEIQEQLEDIDGVTTVDFDDTDEH
                     YTNASALFSVSFDGEVGDAHAEQALQEIRDELEGTYDTSIYSEVGYDSSADLQSEMVV
                     IVIIAAIIIVLVLTLTSRSYAEVPVLIMTFGAAALLNMGTNFLCGTISFISNSVTVIL
                     QLALAIDYAIILCHRFSDEHETMPTREACIAALSKAIPEISSSSLTTISGLAALAFMH
                     FGIGRDLATVLIKAILFSMLCVFTLMPGLLVLFSKLIDKTRHKNLIPKITAVGKFDIK
                     TRFIIPPIFGVIIVAAAVFANLCPYCYTYTDLVTAKQSERQIAYQKIKNTFGSSNMVA
                     VIVPSGDYESEGKILDELDGCAEVKSTMGLANIEAMDGYMLTDAVTPRQLAEMADLDF
                     EVAKALYGAYAVDHDEYGEIINGLDDYKVPIYDMFRFLEQEMHDGNITLSGDVQDTLD
                     DLFDQLDEAQKQLQSDDYSRMVVYLNLPEETDETFAFVNKMHDIIGKYYATDSFYVVG
                     NTTSAMDLSSSFGEDNLLISVLSALFVILVLLFTFKSAGLPVLLIIVIQGSIWINFSV
                     PYLQHTPLYFLGYLIVNSIQMGANIDYAIVISSHYTDLKKVMRPKEAIVTALNESFPT
                     IFTSGSILASAGILISVLTTNPVIAAIGTCLGRGTIVSIMLVLFVLPQILILGDTIIE
                     RTSFEMKGVAGMTHTASGTMHINGRVRGYINGVVDADIKGVLHGQFSANIATGTEFTV
                     DKPDMYLSEENIHESSDAGEETAPDTPAADDAAKGGDAE"
     gene            2660..>5430
                     /locus_tag="ACZPAX_05340"
     CDS             2660..>5430
                     /locus_tag="ACZPAX_05340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113357.1"
                     /translation="MNRIFQLTRRLWAALLALCLVLALTLPVFAEGESSADTTEKETY
                     HIGTMDELLQLADYCRLDSWSENRTVMLDADLELTGSGFSGIPSFSGVFLGQGHIISG
                     LSLVNDGSVVGFFRYVQKGANVRDLVVRGRAMPTGSRTTVGGIAGSNAGTLHNCRFEG
                     VSSGASIVGGIVGTNLASGVIESCTTTGSVYGAHFIGGIAGENHGIIANSTNTANVNT
                     TVEQNDIDLSELTLGDLIGTENVADITDIGGIAGQSGGVIRACMNRGTVGYQHMGYNV
                     GGIAGSQTGYIEGCVNYGTVYARKEGGGIVGQMEPSSTLEYTKDTLQELSDEMGTLQT
                     LVNRACDDASAASSDLSNQLDALKSSVTSSRNAIENLLQQTEDGISISSQTVKTDLSK
                     FKQNLKNTTGDPEDPNATKQPDGAVIDPIYSTGDLTNRNNDDNDDDSDSDIDLPDISL
                     PDIGIDTGDIGKDIPVPESQAEETPTQDEAVTAPEQPADPAPAEQPAEAADTAPVTEK
                     APAGQSAEPEAEREAHGQPDPSTDADSDTTIGPQPTPDPNQDDGGYIVENPENRDFGK
                     ELVDGISDAIPNSVDVEIPKVELTNKDAITASRNDLSSNLTSMGDIVSNLNSNASSNS
                     QALINDVRAISNQINKIGQTLSGASDNIKDPDDIVDDISDEDTDDDVEAKVTNCINSG
                     VVNADINAGGITGAMARENDLDPEDDYTTAGSESLNFTFKTRVVARDCTNYGTVSAKK
                     QNAGGIVGDAEMGSVIDCKGFGTVDAEDATAVGGVAGVLDATNLTGTGNAENLKDITN
                     KSTVVGNAFVGGITGNLYSTTENDSDANTLTGLANQGTVLAGANYQGSTAGQSRVLGQ
                     FFGGIAGYARSLVLDECHSATRSGLSETTLTNLVQAGYNADGTLNENSPLKGDFVGGL
                     VGFGRDVQMT"
BASE COUNT         1196 a         1752 c         1520 g          962 t
ORIGIN      
        1 ttcatcatat ctttgtacat tcccacagat ttgaccgtgt tcccgcgtgc ttgcttttcg
       61 tataataatg atgtatcctt tttatattaa atacaatggg aaagtgaggc tgtataccct
      121 tggagaacga gaagccaaaa ggcaacggca tggaaaaatt ggccgcgttt atcgttgata
      181 agcgcaacct gtttttcctt ttatacatat tcgcgctgat tttctgcgcc atcgccaccg
      241 gctgggtcaa ggtcgaggat gacatcacga cctacctgcc cgacacaacc gagacacgcc
      301 agggcctgac cgtgatgaac gacaacttcg tcacctacgg caccgccaac gtcatggtgt
      361 ccaacatcac ctacgacacg gcgctggaaa tccaggaaca gcttgaggat atcgacggcg
      421 tgaccaccgt tgatttcgac gacaccgacg aacattacac aaatgcgtcg gcgctgttca
      481 gcgttagctt tgacggcgag gtcggcgacg cccacgccga gcaggcgctg caggagatcc
      541 gcgacgagct ggaagggacc tacgacacct cgatttattc cgaggtcggc tacgattcct
      601 ccgccgatct gcagtcggaa atggtcgtca tcgtcatcat cgctgccatc atcattgtgc
      661 tcgtgctgac gctgacctcc cgctcctacg ccgaggtgcc ggtccttatc atgaccttcg
      721 gcgcggccgc gctgctgaac atgggcacca acttcctgtg cggcacgatc agcttcattt
      781 ccaactccgt cactgtcata ttacagctgg cgctggccat cgactacgcc atcattctgt
      841 gccaccgctt cagcgacgag cacgagacga tgcccacccg cgaagcctgt atcgccgcgc
      901 tttccaaggc catcccggag atttcttcct cctcgctgac caccatcagc ggtctggccg
      961 cgctggcgtt catgcacttc ggcatcggcc gcgacctggc tacggtcctg atcaaggcta
     1021 tcctgttcag tatgctctgt gtctttacgc tgatgcccgg cctgctggtg ctgttcagca
     1081 agctgatcga caagacccgc cacaaaaacc tgattcccaa gatcaccgcc gtcggcaagt
     1141 tcgacatcaa gacccgcttt atcatcccac cgatcttcgg cgtcatcatc gtggcggcag
     1201 cggtgttcgc caacctgtgc ccctactgct acacctacac cgatctggtc acggccaagc
     1261 agagcgagcg ccagattgcc taccagaaaa tcaaaaacac cttcggcagc agcaacatgg
     1321 tagccgtcat cgtgccgtcc ggcgattatg agtccgaggg caaaattctc gacgagctgg
     1381 acggctgcgc cgaggtcaaa tccacgatgg gtctggccaa cattgaagcg atggacggct
     1441 atatgctgac cgacgctgtg accccgcgcc agctggccga gatggccgac ctcgacttcg
     1501 aggttgccaa ggcactgtac ggcgcctacg ccgtcgacca tgacgagtac ggcgagatca
     1561 tcaacggtct ggacgattat aaagtgccga tctacgacat gttcaggttc ctggaacagg
     1621 agatgcacga cggcaacatc acgctctcgg gtgatgtgca ggacacgctg gacgacctgt
     1681 tcgaccagct cgatgaagcc cagaagcagc tgcagagtga cgattacagc cgtatggtcg
     1741 tctatttgaa cctgcccgag gagaccgacg agacgttcgc cttcgtcaac aagatgcacg
     1801 acatcatcgg caaatactac gccaccgatt cgttctacgt tgtcggcaat acgacgagcg
     1861 ccatggacct gtccagctcc ttcggcgagg ataacctgct catcagtgtg ctgtccgcgc
     1921 tgttcgttat cctggtcctg ctgttcacat tcaagtccgc gggtctgccc gtgctgctga
     1981 tcatcgtcat tcagggcagt atctggatca acttctcggt gccgtacctg cagcacaccc
     2041 cgctgtactt cctcggctac ctgatcgtca actccattca gatgggtgca aacatcgact
     2101 acgccatcgt tatctccagc cactacaccg acctgaaaaa ggtcatgcgc ccgaaagagg
     2161 ccatcgtcac agccctgaac gagtcgttcc cgaccatctt cacctccggc tccattctgg
     2221 catcggcggg tattctgatc agcgtgctga ccacgaaccc cgttatcgcc gccatcggta
     2281 cctgcctggg ccgcggcacg atcgtttcca tcatgctggt tctgtttgtc ctgccgcaga
     2341 tcctgatcct gggcgacacc atcatcgagc gcacgagctt tgagatgaag ggcgttgccg
     2401 gtatgaccca cacggccagc ggcaccatgc acatcaatgg ccgcgtgcgc ggctacatca
     2461 acggcgtcgt cgatgccgac atcaagggcg tgctgcacgg ccagttcagt gcgaacattg
     2521 ccaccggcac cgaatttacc gttgacaagc ccgacatgta cctgtcggaa gaaaacatcc
     2581 atgaatccag cgacgccggg gaagaaaccg ccccggatac ccccgccgct gacgacgctg
     2641 cgaaaggagg cgacgccgaa tgaaccgtat tttccaactg acgcgccgcc tttgggccgc
     2701 gctgctggcg ctctgccttg tgctggcttt gaccctgcct gtctttgcgg aaggggagag
     2761 cagcgccgac accactgaaa aagagactta ccacatcggt accatggacg agcttttaca
     2821 gctggccgac tactgccgcc tggacagctg gagcgagaat cgtaccgtca tgctggacgc
     2881 tgacctggaa ctgaccggaa gcggctttag cggcatcccg tccttcagcg gcgtgttcct
     2941 gggccagggg cacattattt ccggcctgag cctggtgaat gacggctcgg tcgtgggctt
     3001 cttccgctat gtgcagaaag gcgccaacgt ccgcgacctc gttgtgcggg gccgcgccat
     3061 gcccaccggc agccgcacca ccgtgggcgg cattgcgggc agcaacgccg gcaccctgca
     3121 taactgccgg tttgagggcg tatcctccgg tgcgtccatt gtcggcggca ttgtcggcac
     3181 gaacctagcc tccggcgtca tcgagagctg caccaccacc ggcagtgttt acggtgcgca
     3241 cttcatcggc ggtattgccg gtgagaacca cggcatcatt gccaactcca ccaacacagc
     3301 caatgtgaac acgacggtgg agcagaacga tattgacctc tctgagctga cgctgggtga
     3361 cctgatcggc accgagaacg tggccgatat caccgatatc ggcggcatcg cgggccagtc
     3421 cggcggcgtt atccgcgcct gcatgaaccg cggcaccgtg ggctaccagc acatgggcta
     3481 caacgtcggc ggcattgcgg gcagccagac cggctacatc gagggctgcg tcaactacgg
     3541 caccgtctac gcccgcaagg agggcggcgg catcgtcggc cagatggagc ccagcagcac
     3601 gctggaatac accaaagaca cgctgcagga actcagcgat gagatgggca ccctgcaaac
     3661 gctggtcaac cgcgcctgcg acgacgccag cgccgccagc agcgacctgt ccaaccagct
     3721 ggacgcactg aaaagcagcg tgaccagctc ccgcaacgcc atcgagaacc tgctgcagca
     3781 gaccgaggac ggcatttcca tcagcagcca gaccgtcaag acggacttga gcaagttcaa
     3841 gcagaatttg aaaaacacca cgggcgaccc ggaggacccc aacgccacca agcagcctga
     3901 cggcgcggtc atcgacccca tctactccac aggcgacctg acgaaccgca ataatgacga
     3961 caacgacgac gattcggaca gtgacatcga tctgcccgac atcagcctgc cggatatcgg
     4021 tatcgacaca ggcgatatcg gtaaggatat cccggtgccc gagagtcagg ccgaggagac
     4081 cccgacgcag gacgaggccg tcacagcccc cgagcagccc gcagacccgg ccccggctga
     4141 acagcctgcc gaagcggcgg acaccgcccc tgtgaccgag aaggcccccg ccgggcagag
     4201 cgcggaaccc gaagctgagc gcgaagccca cggccagccc gaccccagca cggatgccga
     4261 cagcgatacc accatcggcc cccagcccac gcccgacccg aaccaggacg acggcggcta
     4321 tatcgtcgag aatccggaga accgggactt cggcaaggag ctggtggacg gcatcagcga
     4381 cgccattcca aactccgtgg atgtggagat ccccaaagta gagctgacga acaaggacgc
     4441 catcacggcc agccgcaacg atttgagcag caacctgacc agcatgggcg atatcgtcag
     4501 caatctgaac tccaacgcaa gcagcaactc ccaggccctg atcaacgatg tgcgcgccat
     4561 ttccaaccag atcaacaaga tcggccagac gctgtccggt gccagtgaca acatcaagga
     4621 cccggatgac attgtggacg atatctccga cgaggacacc gacgacgatg ttgaggccaa
     4681 ggtcacgaac tgcatcaaca gcggtgtggt caacgccgat atcaacgcag gcggcattac
     4741 cggggccatg gcccgggaga acgacctgga ccccgaggac gactacacca ccgcaggcag
     4801 cgagtcgctg aatttcacct tcaagacccg cgttgtggcc cgcgactgca cgaactacgg
     4861 caccgtcagc gccaaaaagc agaacgcggg cggcatcgtg ggtgatgcag agatgggttc
     4921 cgtcatcgac tgcaaaggct ttggcaccgt cgatgccgag gacgccactg ccgtgggcgg
     4981 cgtggccggt gtactggacg ccaccaacct gaccggcacc gggaacgccg aaaacctcaa
     5041 ggacatcacc aacaagtcca ccgtggtggg caatgccttc gtgggcggca tcacgggcaa
     5101 cctgtactcc accaccgaga acgacagcga cgccaacacc ctgaccggcc tggccaacca
     5161 gggcactgtg ctggccgggg ccaactacca gggcagtacc gcaggccaaa gccgcgtgct
     5221 gggccagttc ttcggcggca ttgcgggcta tgcgcgcagc cttgtactgg atgagtgcca
     5281 cagcgccacc cgcagtggcc tgagtgagac gaccctgacc aaccttgtgc aggcgggcta
     5341 taacgccgac ggtacgttga acgagaacag ccccctcaag ggcgactttg tcggcggcct
     5401 ggtgggcttt ggccgcgacg tccagatgac
//
LOCUS       DBMDSQ010000113         5417 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_3698_length_5417_cov_2.328442, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000113 DBMDSQ010000000
VERSION     DBMDSQ010000113.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 5417)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5417)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5417
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3698_length_5417_cov_2.328442"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..600
                     /locus_tag="ACZPAX_05345"
     CDS             <1..600
                     /locus_tag="ACZPAX_05345"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015537643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005543 - phospholipid binding [Evidence
                     IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0006593 - L-ornithine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HIR1113358.1"
                     /translation="KQTTLDALAKYSGIPVISGMTDYAHPLQVLTDLMTVRERKGTLA
                     GLKAGFIGDGYNMANSVIVGCLAMGMAVTAACPKHYRPAADVLMLAQRYGDKFTLTND
                     PDEAARDADVLFTDVWVSMGMEREAEQRRRDFTGFTLDAARMALAKPDCMVQHPLPAH
                     RGEEIAPDVLEGHMEEIFDEAENRIYVEKAVLAILLAGK"
     gene            720..1361
                     /locus_tag="ACZPAX_05350"
     CDS             720..1361
                     /locus_tag="ACZPAX_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013840131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HIR1113359.1"
                     /translation="MSKQTLQQKIQRVLARFTPRRIAIIMLGTAILSFGIHNIHQRTA
                     ITEGGIIGLVLLINHWLGVPASVASPLLDAISYALAFKFLGWDFLLSAGIASISLAGF
                     YRLWECFPPMLPDLTPWPLAAALLGGVFVGVGVGLIVRQGGSSGGDDALALVIHKLTG
                     WRLSRAYLFTDLTVLFASLSYIPFRRIAYSLVTVTVSSLLIDYIKDFRTKTNV"
     gene            1450..2076
                     /locus_tag="ACZPAX_05355"
     CDS             1450..2076
                     /locus_tag="ACZPAX_05355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113360.1"
                     /translation="MKLTRKLAALAMAVLLAVSLTACSPKEWAQNVVVHLVHKLGLVE
                     ESDEEDTDTTTKAPAGGSVEFPAEMDTDSARVVTYPMDDTLYVSFNGIANRSTEYFVA
                     GGDSMTVTGYASTEASSENYMYFKIAFWELSDDKTMTSYVPDSTIYFRADEADYQYTI
                     TGLTAGKQYKITISYDNGNKYYVTGGLKVEGLGSTELNTYGDEETTNS"
     gene            2201..3106
                     /locus_tag="ACZPAX_05360"
     CDS             2201..3106
                     /locus_tag="ACZPAX_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007048226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR1113361.1"
                     /translation="MDKKQLRNTLLLVCGAFIWGTAFVAQSVGSDYMGPYTFLAGRSW
                     LACAFLLGLMAVLRKMGRTNTAAEPGFWQNKKVLVKGGIFCGAALFAASAAQQMGIGT
                     TSTAKAGFMTALYVVLVPVTSIFFGSRPGVKLWGCVVVSVVGLYLLCLAGRDTLSLTG
                     GEWQLLLCAALFTLQIMLVNHFSPQLDGIQLSFMQFLVVAVLSTVFMFAFETPTIEQF
                     RGAAVSIAYCGIMSSGVAYTLQIVGQKELDPTIASLAMCLESVFSALAGWLILGETLS
                     ATELCGCVLMFGAIVASQLPEKKRG"
     gene            3217..4968
                     /locus_tag="ACZPAX_05365"
     CDS             3217..4968
                     /locus_tag="ACZPAX_05365"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1113362.1"
                     /translation="MQDLRMIFGYGKPYRKDLFAAVGLIFVECIFEMVIPVLMSTLVD
                     DGVPDHNMAVIFRQGGLMILCAVLALITGLLYARYAARFANGFASELRLAEYAAVQKF
                     DFANLDRFSNASLVTRMTTDVTVMLNAVNTGLRPLVRSPVMLGMGLAMACVLSPRLTL
                     VFLITTPILAAVLAWIVSKVGPLYGRQQSAVDHLNGRIQESLTAIRAIKAFVRGDYEN
                     DQFNTVNAELSDASTETFRYAVLNLPAFQAVMYTAIVCILWFGGGFVLHGTMSVGQLT
                     AFLSYVLQVLNSVMMFSGVFLQMSRSLASARRIREVLTEQPDLANAAAPVETIPDGRI
                     DFDHVSFKYNAKAEKNALSDITLHIPAGATVGIIGGTGAAKTTLVQLIPRLYDATEGT
                     VKVGGHDVRDYDLAALRDAVGIVLQKNLLFSGSIRDNLKWGSPDATDDDLWAACRAAH
                     ADEFLNRMPQGLDTDLGQGGCNVSGGQKQRLCIARTLLKHPKVLIFDDSTSAVDTATE
                     SGIRRALAELGDVTKLIIAQRISSVQSADIIVILDDGRLHAVGTHDELLKKDTIYQEI
                     YNSQMKGGDADGESIRR"
     gene            4946..>5417
                     /locus_tag="ACZPAX_05370"
     CDS             4946..>5417
                     /locus_tag="ACZPAX_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007048224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HIR1113363.1"
                     /translation="MAKAFGGKRPQNLRHTLRRFGDYLGHHKRKLALIGVLTSISALA
                     ALIGTYMIRPAVNGILENGSLAYLAGVVAFTAFVYLIGALSTLGYTQLMVRTAQRITQ
                     EIRSDLFAHLQTMPLNFFDTNRKGDLMSLFTNDVDTISDALNNSFAVVIQMFIQI"
BASE COUNT         1016 a         1661 c         1643 g         1097 t
ORIGIN      
        1 aagcagacga ccctggacgc gctggcaaaa tacagcggca taccggtgat ttccggcatg
       61 actgactacg cccacccctt gcaggtgctg acggacttaa tgaccgtgcg tgagcgcaag
      121 ggcacgctgg ccgggctgaa ggccgggttc atcggcgacg gctacaacat ggcgaacagc
      181 gtcattgtgg gctgccttgc catgggtatg gcggtcacgg ccgcctgccc gaagcactac
      241 cgcccggcag ccgatgtgct gatgctggcg cagcggtacg gcgataaatt cacgctgacg
      301 aacgaccccg acgaggccgc ccgcgatgcg gacgtgctgt ttaccgatgt ctgggtcagc
      361 atgggcatgg agcgcgaggc cgagcagcgc cgccgggatt tcaccggctt tacgctggat
      421 gcggcccgca tggcgctggc caagccggac tgcatggtgc agcacccgct gcccgcccac
      481 cgcggcgagg agatcgcccc cgatgtgctg gaagggcaca tggaggagat ttttgacgag
      541 gccgagaacc ggatttacgt ggaaaaggcc gtgctggcaa ttttgctggc ggggaaataa
      601 agtacaggaa gcagggcaga gtgcggccct gcttttttgt tggggcgaga ggggctttcg
      661 ccgccttccc ttgacaggct gtgttatact ggatatatca ctgtccaagg agtgccgcta
      721 tgtcaaaaca aacgctgcaa caaaaaatcc aacgtgtact ggcccggttc acgccgcggc
      781 gcattgccat tatcatgctg ggcacggcga tactctcctt cggcatccac aacatccacc
      841 aacgcaccgc catcacggag ggcggcatca tcggactggt gctgctcatc aaccattggc
      901 tcggcgttcc ggcgtcggtg gcgtcgcccc tgttggatgc cataagttac gcgctggcgt
      961 ttaaattttt ggggtgggat ttcctgctct cggcgggtat cgccagcatc agcctggcag
     1021 ggttctaccg cctgtgggag tgttttcccc cgatgctgcc tgacctgacg ccctggccgc
     1081 tggcggcggc gctgctgggc ggtgtgttcg tcggcgtggg cgtcgggctg atcgtgcggc
     1141 agggcgggtc cagcggcggc gacgacgcgc tggccctcgt catccacaag ctgaccggct
     1201 ggcgactctc ccgcgcgtac ctttttaccg atttaacggt gctttttgcg tcgctgtcct
     1261 acattccgtt ccggcgcatc gcctattcgc tggtcacggt cacggtgtcg tcgctgctca
     1321 tcgactacat caaggatttc cgcacaaaaa caaatgtgta aaaaccgccg gaacgcttgt
     1381 acacacggga attttgcggt ataataatct gtgtattttt gcagacatca cccattggag
     1441 gtttacgata tgaaacttac ccgcaagctc gcggcgctgg ctatggccgt gctgctggca
     1501 gtcagcctga cggcctgctc ccccaaagag tgggcacaga atgttgtcgt ccatctggtc
     1561 cacaagctgg gcctggtcga ggaatccgac gaggaggaca ccgacaccac cacgaaagcc
     1621 cccgcaggcg gcagcgtgga gttccccgcc gagatggata ccgactccgc ccgtgtggtg
     1681 acttacccga tggatgatac gctgtatgtc tccttcaacg gcattgccaa ccgctccact
     1741 gagtatttcg tcgcgggcgg cgacagcatg accgtgaccg gctatgcgtc taccgaggcc
     1801 agcagcgaga actatatgta cttcaagatc gcgttctggg agctttccga cgataagacg
     1861 atgacaagct atgtgccgga ttcgacgatc tacttccgcg ccgacgaggc tgactaccag
     1921 tacaccatta ccggcctgac cgcaggcaag cagtacaaaa tcaccatcag ctatgacaac
     1981 ggcaacaaat actatgttac cggcggcctg aaggtggagg gcctgggcag caccgagctg
     2041 aacacctacg gcgacgagga aaccaccaac agttaaaaac aggcaacaat gccgcccgcg
     2101 ccgcagatgg ccgggcggca tttttgatga tgcatcgtag gggccggtgc tcgccatcgg
     2161 cccgcaggaa agtacaacac agagggaaaa gggaaaaatt atggacaaaa aacagctgcg
     2221 caacacctta ttgctggtgt gcggcgcatt catctggggc acagctttcg tcgcgcagag
     2281 cgtcggcagc gactacatgg ggccgtacac gtttttggcg ggccgcagct ggctggcctg
     2341 cgcgttcctg ctggggctga tggctgtgct gcgcaagatg ggcaggacga acaccgccgc
     2401 cgagccgggg ttctggcaga acaaaaaggt gctggtcaag ggcggtattt tctgcggtgc
     2461 ggcgctgttt gcggcgtcgg cggcgcagca gatgggcatt ggcaccacca gcacggccaa
     2521 ggctgggttt atgacggcgc tctacgtggt gctggtgccg gtcacgagca tcttttttgg
     2581 cagccgcccc ggcgtcaagc tgtggggctg cgtggtggtc agtgtggtgg ggctgtatct
     2641 gctctgcctg gcaggccgcg ataccctgag cctgaccggc ggcgagtggc agctgctgct
     2701 ctgcgcggcg ctgttcacgc tgcaaatcat gctggtcaac catttcagcc cccagctgga
     2761 cggcatccag ctgagtttta tgcagttctt agtggtggcg gtgctgtcca ctgtgtttat
     2821 gtttgcgttt gaaacgccga ccatcgagca gttccgcggc gcggcggtgt ccattgccta
     2881 ctgcggcatc atgtccagcg gcgtggccta cacgctgcag atcgtcggcc agaaggagct
     2941 ggacccgacg attgccagcc ttgccatgtg cctggagagt gtgttcagcg ccctggccgg
     3001 ttggctgatt ttgggcgaga cgctgtccgc caccgagttg tgcggctgcg ttttgatgtt
     3061 tggggccatt gtggcgagcc agctgccgga gaaaaagcgc ggctaaaaag gcccccttgt
     3121 caaaggggac tgtccgagcc tgcgaggtcc ggggctttgc agccttccca tcgttaccca
     3181 ttcccttccc gcataacgtc ccaaaggagg tctcctatgc aggacctgcg catgattttt
     3241 ggttacggta aaccctaccg caaagacctg tttgcggcgg tggggcttat ctttgttgaa
     3301 tgtatctttg aaatggtcat ccccgtgctg atgtctaccc tggtggacga cggcgtgccg
     3361 gaccacaata tggccgtcat cttccgacag ggcgggctga tgattctctg cgcggtgctg
     3421 gcgctcatta ccggtctgct ctatgcccgg tacgccgcgc ggtttgccaa cggctttgcg
     3481 tcggagctgc gcctggcgga gtatgccgcc gtgcagaaat tcgacttcgc gaaccttgac
     3541 cgtttctcca atgcgtccct cgtcacccgc atgaccaccg acgttaccgt catgctcaac
     3601 gctgtcaata ccggcctgcg gccgttggtg cgcagcccgg tcatgctggg catgggcctt
     3661 gctatggcct gcgtgctcag cccgcggctg acgctggttt tcctcatcac aacgccgatc
     3721 cttgctgccg tgctggcttg gatcgtctcg aaagtcggcc cgctctatgg ccgccagcag
     3781 agcgctgtgg accacctcaa tggccgcatc caggaatccc tcaccgccat ccgcgccatc
     3841 aaggctttcg tgcgcggcga ctatgagaac gaccagttca acaccgtcaa cgccgaactc
     3901 agcgacgcct cgaccgagac gttccgctat gccgtgctca acctgcccgc attccaggcc
     3961 gtcatgtaca cggccatcgt ctgcattctc tggttcggcg gcggctttgt gctgcacggc
     4021 accatgagcg tcggccagct gaccgccttt ttgagctacg ttttgcaggt gctcaactcg
     4081 gtcatgatgt tctccggcgt gtttctccag atgtcccgtt cgctggccag cgcccgccgc
     4141 atccgcgagg tgctgacaga gcagcctgat ttggccaacg ccgccgcgcc ggtggagacg
     4201 atccccgacg gccggatcga ttttgaccac gtcagcttca aatacaacgc aaaggcggag
     4261 aaaaacgccc tgtccgacat cacgctgcac atccccgccg gtgcgacggt gggcattatc
     4321 ggcggcaccg gcgcggccaa aacgacgctg gtgcagctga tcccccgcct ctacgatgcc
     4381 accgagggca ctgtcaaggt cggcgggcat gacgtgcgtg actacgacct ggccgctctg
     4441 cgggatgctg tgggaattgt gctgcaaaag aacctgctgt tctccgggtc gatccgcgac
     4501 aacctcaaat ggggcagccc cgatgccacc gacgacgacc tgtgggccgc ctgccgcgcc
     4561 gcccacgccg atgaatttct gaaccgtatg ccccagggcc ttgacaccga cctgggccag
     4621 ggcgggtgca atgtctccgg cgggcagaag cagcgtcttt gcattgcccg cacgctgctc
     4681 aaacacccca aggtgctgat ttttgacgac tccaccagcg ccgtggatac ggccaccgag
     4741 tccggcatcc gccgcgcgct ggccgagctg ggcgacgtga ccaagctcat cattgcccag
     4801 cgcatctcgt ctgtccagag cgcggacatc atcgtgattc tggacgacgg ccgtttgcac
     4861 gccgttggca cccatgacga actgctgaaa aaggatacga tctatcagga aatttacaat
     4921 tcccagatga aagggggcga cgcagatggc gaaagcattc ggcggtaagc gcccgcaaaa
     4981 cctgcgccac accctgcgcc gcttcggcga ttacctaggc caccacaagc gcaaactggc
     5041 actgatcggc gtgctgacgt ccatcagcgc cctggccgcc ctgattggca cctacatgat
     5101 acgcccggcc gtcaacggca ttctggaaaa cggcagtctt gcctacctgg ccggggtcgt
     5161 ggcgttcacg gcgttcgtct acctcatcgg cgcgctgtcc accctgggct acacccagct
     5221 catggtccgc acggcgcagc gtatcaccca ggaaatccgc agcgacctgt ttgcccacct
     5281 gcagacgatg cccctgaact tctttgacac gaaccgcaag ggcgatttga tgagcctgtt
     5341 caccaacgat gtggacacga tttccgatgc gctgaacaac agctttgcgg tggtcattca
     5401 gatgtttata cagatcg
//
LOCUS       DBMDSQ010000114         5375 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_3726_length_5375_cov_1.731513, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000114 DBMDSQ010000000
VERSION     DBMDSQ010000114.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 5375)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5375)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5375
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3726_length_5375_cov_1.731513"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(224..1075)
                     /gene="folD"
                     /locus_tag="ACZPAX_05375"
     CDS             complement(224..1075)
                     /gene="folD"
                     /locus_tag="ACZPAX_05375"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004620258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HIR1113364.1"
                     /translation="MGATIIDGKALAAATKAEAAEKVKELKAQGINPCLAVLLVGENP
                     ASQVYVRGKINDCAQCGIESCSINLPADTTQETLLAEVKKLAADPAVHGILVQLPLPA
                     QIDEKAVIDAIPPEKDVDGFSPVNVGRMQIGEPCYLPCTPAGCIRMIESTGIDIGGKN
                     AVVIGRSNIVGKPAALLLLAKNATVTICHSKTKNLKEVCAAADILVAAVGREGFVTGD
                     MVKPGAVVIDVGINRGADGKLHGDVNFEEAAEKAAYITPVPGGVGPMTRAMLMLNTVE
                     AAKRQAM"
     gene            complement(1179..2216)
                     /gene="lgt"
                     /locus_tag="ACZPAX_05380"
     CDS             complement(1179..2216)
                     /gene="lgt"
                     /locus_tag="ACZPAX_05380"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005933951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HIR1113365.1"
                     /translation="MVYHVQFPGLGLDLTVNRVALAIGGFNIYWYGVIIAAGMLLAML
                     YAFRNAVDYGIDSDRLVDVVAIGAVMSIVCARIYYVAMAPFEYQSLWEMVDIRKGGIA
                     IYGAVIGAFVFGGLAAKWRKVPLLPLFDLVSLGFLIGQGIGRWGNFVNQEAFGTNTTL
                     PWGMYSEGTEAYLKSVQVTLQAGVTVDPTAPVHPTFLYESIWCLLGFLVLSHFFKKRR
                     FNGQIFLSYIIWYGLGRAWIEGLRTDSLLIGNTGLRASQLVAIGSVIVAAMLMVIGLR
                     SAKGRELTVTLAVKDIKKQAEARDRFAPDTLPANAPHAEFVKATDAMNERLDALDLNE
                     VEIEEIEEPKE"
     gene            complement(2233..3531)
                     /gene="yfmH"
                     /locus_tag="ACZPAX_05385"
     CDS             complement(2233..3531)
                     /gene="yfmH"
                     /locus_tag="ACZPAX_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P 5-aminopentanol modification-associated
                     protein YfmH"
                     /protein_id="HIR1113366.1"
                     /translation="MLNNDHKNFAAAQARAAAAVNCESVVLPSGLTVLCRTMPGYSSV
                     HAIYATGFGSIHRAFKLDGKQVTLPAGTAHFLEHKMCETPKGDSFTFYAKTGASANAF
                     TSYDRTCYLFSATQKINENLDILLGMVGKPWFTKATIEKEQGIIGQEIKMYDDSPDWR
                     LLNALFRCLYADHPLRDDIAGTVESIAELTPQMLYNCTKAFYAPSNMVLSVAGKITLA
                     QAVDACKRNGLYRARAPHEVEWDIPAQDGPLPHKEALFTMPVTKPCFGVAYREEPLAP
                     GDLKRELLLDMLGDLVVGGLTKLYRRLYDEALVNPEFSGDFIAVRGACAVAFTGESDT
                     PRQVVDLLQEEIERMRREGVDHEVFMLVKNQMYGELLGDVEAVDDAAEEAAAACLKGR
                     TLADEIAALAELTAEDANALMQTALREENRAYVQIDPAEK"
     gene            complement(3521..4822)
                     /locus_tag="ACZPAX_05390"
     CDS             complement(3521..4822)
                     /locus_tag="ACZPAX_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009322449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HIR1113367.1"
                     /translation="MERKQLAPGVCITTLDAAKFNRCRITLHLRFPARRESATDAAVL
                     ALVLERGYAACPDMTALSRRLAELYGADLGVDLASAGTDRVLSADICGIKDAYALAGE
                     NLTDAYADIVFGTIFDPYLVDGAFDPEAVRIETETQARRLEAEFNSKRLYCVRQARRK
                     FYGDTAAGIELGGYPGELVNVTPQSLKAEYDRILSTAYIDVMVQGVDEDRVADMLLKK
                     LDGIRRDPHPFAAPIAMPATPLRHFKEEVPGLTQAKLCMLFTTGEEHPNPPSVSILRV
                     AMSVLGGSATSRLFRNVREKQSLCYYCGSAAQRATGVMMIDSGVEPGKEQQAEAAIIA
                     ELEGLKNGPITQEEVDDCCRGLLSSMDALGDSLAALENWYYGQITRGEPLYPPEYGKV
                     LTSAVTLDEVRQTLQSYSYSVCYAVTAEPGTQGKGGSEDVE"
     gene            5069..>5375
                     /locus_tag="ACZPAX_05395"
     CDS             5069..>5375
                     /locus_tag="ACZPAX_05395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HIR1113368.1"
                     /translation="MDCFALKGTFIDTPTPDALRVREGYLLCENGLCAGFSRTAPAGV
                     EILDYTGRIITPGLVDLHLHAPQYSYCGTAMDLELLDWLQQYTYPEESHYADADYARA
                     "
BASE COUNT          990 a         1673 c         1652 g         1060 t
ORIGIN      
        1 tagtattcta tcatagaact gcacagcttg tcaaggggtt ttcgggcgtg ggaaggttaa
       61 aatttcagaa tttcttttgc cttcccccct gggggggaag gtggccccgc agggccggat
      121 gaggggcaag tctgcggttg tatcccatca gtgggctgca atggcaaggt tacccctcat
      181 cagtccgctt cgcggacagc ttcccccaag ggggggggag cctttacatg gcctgccgct
      241 ttgcggcctc cactgtgttc agcatcagca tggcgcgggt catggggccg acaccgccgg
      301 gcacgggggt gatgtaggcg gccttctcgg cggcttcttc aaagttcacg tcgccgtgca
      361 gcttgccatc cgcaccacgg ttgatgccga catcgatgac caccgcaccg ggcttgacca
      421 tatcgccggt cacaaagccc tcgcggccca cggcggccac caaaatatcc gcagcggcgc
      481 agacctcttt gaggtttttg gtcttagagt gacagatggt cacggtcgcg ttcttcgcca
      541 gcagcagcag cgccgcaggc ttgcccacga tgttggagcg gccgatgacc acagcgtttt
      601 tgccgccgat atcgatgccg gtggactcga tcatgcggat gcagcccgcc ggggtgcagg
      661 gcaggtagca cggctcgccg atctgcatac ggcccacgtt gaccggcgaa aagccatcca
      721 catccttctc cggcgggatg gcgtcaataa cggctttctc gtcgatctgc gcaggcagcg
      781 gcagctggac aaggatgccg tgtacggcgg ggtcggcggc cagctttttt acctcggcca
      841 gcagggtttc ctgggtcgta tcagcgggca ggttgatgga acagctctcg atgccgcact
      901 gggcgcagtc gttgatcttg ccgcgcacgt acacctggct ggcggggttt tcccccacca
      961 gaaggaccgc cagacagggg ttgataccct gggctttcag ttccttgacc ttctctgcgg
     1021 cttctgcctt ggtggccgcg gccagcgcct tgccgtcgat gatagttgct cccataatag
     1081 ttccctcttt caaatgtatt ttttgtccgc cggggcgggc aaaacggtgt cgggtcttac
     1141 accagcggca cctatcgccg tgccgcagaa gcgtctgttt attccttcgg ttcttcgatc
     1201 tcttcgatct cgacctcgtt caggtccagc gcgtccagcc gctcgttcat ggcgtcggtc
     1261 gccttgacaa actcggcgtg gggcgcattc gcaggcaacg tatccggcgc gaagcggtct
     1321 cttgcctcgg cctgcttttt gatatccttc acggccagcg tgacggtcag ctccctgccc
     1381 ttggcgctgc gcagaccgat gaccataagc atcgccgcca cgatgaccga gccgatggcc
     1441 accagctggc tggcccgcag gccggtattg ccgatgagca gcgagtcggt gcgcaggccc
     1501 tcgatccagg cgcggcccaa gccgtaccag atgatgtagc ttaagaaaat ctggccgttg
     1561 aaacgacgct ttttgaaaaa gtgggacagt accagaaacc ccagcaggca ccagatgctc
     1621 tcatacaaaa acgtcgggtg taccggtgcg gtgggatcca ccgtaacacc tgcctgcagc
     1681 gtgacctgca cggatttgag atacgcctcg gtaccttcgc tgtacatgcc ccagggcagc
     1741 gtcgtgttcg tgccgaaggc ctcctgattg acaaagttgc cccagcggcc aatgccctgc
     1801 ccaatcaaaa agcccagcga aacgaggtca aacaacggca gcagcggcac cttgcgccat
     1861 ttggcggcca ggccgccgaa cacaaacgca ccgatgaccg ctccgtagat ggcgatgccg
     1921 cccttgcgga tatccaccat ctcccacagg ctttgatact caaaaggtgc catagccacg
     1981 taataaatgc gggcacagac aatggacatg accgcgccga tggccacaac gtccaccagc
     2041 cggtccgagt cgatgccgta gtccacggcg ttgcggaagg cgtagagcat cgccagcagc
     2101 atacctgccg cgatgatgac gccgtaccaa taaatgttga acccgccgat ggccagcgcc
     2161 acccggttca cggtcaggtc aaggcccaac ccgggaaatt gcacatgata gaccatttta
     2221 tacttatcct cgttattttt ctgccggatc gatctgcacg taagcacggt tttcttcccg
     2281 cagggctgtc tgcatcaagg cattggcatc ctcggcggtc agctcggcca gcgcggcgat
     2341 ctcatcggcc agcgtacggc ccttcaggca ggcagcggcc gcttcctcgg cggcatcgtc
     2401 cacggcttcc acgtcgccca acagctcgcc gtacatctgg tttttcacca gcataaagac
     2461 ctcgtggtcc acaccctcgc ggcgcatccg ctcgatttcc tcttgcagca gatccacgac
     2521 ctggcgcggg gtgtcgctct cgccggtaaa ggccacggcg caggcgccgc gcaccgcgat
     2581 aaagtcaccg gagaactcgg ggttgaccag cgcttcatca tacaggcggc ggtagagctt
     2641 cgtcaagccg ccgacaacca gatcgcccag catatccagc agcagttccc gcttcaaatc
     2701 gcccggggcc agcggctcct cacggtaggc cacgccgaag cagggcttcg tgaccggcat
     2761 tgtgaaaagc gcctctttgt ggggcagcgg gccgtcctgg gcgggaatgt cccactcgac
     2821 ctcgtgcggg gcgcgggcac ggtagaggcc gttgcgcttg caggcgtcaa cagcctgggc
     2881 cagcgtgatt ttgcccgcga cgctcagcac catgttggac ggcgcgtaga acgccttggt
     2941 gcagttgtag agcatctggg gcgtcagctc ggcaatgctc tccaccgtgc cggcgatatc
     3001 gtcccgcagc gggtggtcgg cgtacaggca gcggaacagt gcgttcagca gccgccagtc
     3061 ggggctgtcg tcgtacatct tgatttcctg gccgatgatg ccctgctctt tttcaatcgt
     3121 ggctttcgtg aaccacggct tgcccaccat gccaagcagg atatcgaggt tttcattgat
     3181 tttctgggtc gccgagaaga ggtagcaggt gcggtcatag ctggtaaagg cgttggccga
     3241 cgcgccggtc ttggcgtaga aggtgaagga gtcgcccttg ggcgtctcgc acattttgtg
     3301 ctcaaggaag tgcgccgtac cggcgggcag cgtgacctgc ttgccatcca gcttaaacgc
     3361 gcggtgaatc gagccaaagc ctgttgcata gatggcatgg acgctgctgt acccgggcat
     3421 cgtgcggcag agcacggtca ggccgctggg cagcacaacg ctctcacagt tgacggcggc
     3481 agcggcgcgg gcctgggcgg cggcaaagtt tttgtggtcg ttattcaaca tcttcactgc
     3541 cccctttccc ctgggtaccc ggctcggcgg tcacggcgta gcagacggaa tagctgtaac
     3601 tttgcagcgt ctggcgcacc tcgtccagcg tcacggcgct ggtcagaacc ttgccgtact
     3661 cgggcgggta gagcggttcg ccgcgggtga tctggccata gtaccagttt tccagcgcgg
     3721 ccaggctgtc gcccagggcg tccatgctgg aaagcaggcc gcggcagcag tcgtccactt
     3781 cctcctgggt gatggggccg tttttcagcc cctccaactc ggcaatgatg gcggcctcgg
     3841 cctgctgctc cttgcccggc tcaacgccgg agtcgatcat cataacgccg gtggcccgct
     3901 gggcggcgct gccgcagtaa tagcacaggg actgcttctc gcgcacgttg cggaacaggc
     3961 gactggtggc ggagccgccc agcacgctca tcgccacgcg caggatgctg acgctgggcg
     4021 ggttggggtg ttcctcgccg gtggtaaaca gcatacacag cttggcctgg gtcaggccgg
     4081 ggacttcctc tttaaaatgg cgcagcggcg tggcgggcat ggcgatgggt gcagcgaagg
     4141 ggtgcgggtc acggcggatg ccatccagct ttttcagcag catatcggcc acgcggtcct
     4201 cgtccacgcc ctgcaccatc acgtcaatgt acgcggtgga gaggatgcgg tcatactcgg
     4261 ccttgaggct ttgcggcgtg acgttgacca gctcgccggg gtagccgccc agctcgatgc
     4321 ccgcagcggt gtcgccgtag aacttgcggc gggcctgccg cacgcagtag agccgctttg
     4381 agttgaactc agcttccaag cggcgggcct gggtttcggt ctcgatgcgc acggcctcgg
     4441 ggtcgaacgc gccgtccaca aggtaggggt caaagatcgt gccgaacacg atatccgcgt
     4501 aggcgtcggt caggttctcc cctgccaggg cgtaggcgtc cttgatgccg cagatatcgg
     4561 cggacagcac gcggtcggtg cccgcgctgg caaggtcgac gcccaaatca gcgccgtaca
     4621 gttcggccaa acggcgggac agggcggtca tatcggggca ggccgcatag ccgcgctcca
     4681 gcacaagggc cagcacggcg gcatcggtgg cggactcccg ccgggccgga aaacgcagat
     4741 gcagcgtgat acggcagcgg ttaaatttcg cagcgtccag tgtcgtgata cagacaccgg
     4801 gcgcgagttg tttacgttcc aaatcgcttt tcctttcaaa catcaataga tattggtatt
     4861 ataccgcttt aacggcataa agtaaacaaa aaacttgcaa cagatactat atattgttta
     4921 taatagagat tatgaaagcc ctctggccgc cgcggcgggg tgagggcctc ccgccctgcg
     4981 cccccatgaa aaggggcatc caccgtacac ttgcgggccg ggcatgcccg gcccctacaa
     5041 atcaatttta tatagagagg tcatttctat ggattgtttc gccctgaaag gcacctttat
     5101 agacaccccc accccagacg cactgcgcgt gcgcgagggc tatctgctgt gcgagaacgg
     5161 cctgtgcgcc gggttcagcc gcaccgcgcc cgccggggtg gaaatccttg actacaccgg
     5221 ccgcatcatc acgccgggcc tggtcgacct gcacctgcac gcgccgcagt acagctattg
     5281 cggcacggcc atggacctgg aactgctgga ctggctgcag cagtacacct accccgagga
     5341 atcccactat gccgatgccg actatgcccg cgcgg
//
LOCUS       DBMDSQ010000115         5362 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_3736_length_5362_cov_1.501996, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000115 DBMDSQ010000000
VERSION     DBMDSQ010000115.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 5362)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5362)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5362
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3736_length_5362_cov_1.501996"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            49..189
                     /locus_tag="ACZPAX_05400"
     CDS             49..189
                     /locus_tag="ACZPAX_05400"
                     /inference="COORDINATES: protein motif:HMM:NF024949.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR1113369.1"
                     /translation="MLIIGERIKMLRESHNLSQAALAKRLGITRSIWGCCGALPIPST
                     AL"
     gene            135..275
                     /locus_tag="ACZPAX_05405"
                     /pseudo
     CDS             135..275
                     /locus_tag="ACZPAX_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019161057.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015655 - alanine:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0032328 - alanine transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
     gene            306..1787
                     /locus_tag="ACZPAX_05410"
     CDS             306..1787
                     /locus_tag="ACZPAX_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006356183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HIR1113370.1"
                     /translation="MPPIIAILLALLTKEVYLSLFAGCALGALLVAEFHPWLAFDTLF
                     NTMINSVDFSILMFMILLGMVVMLMQESGGTRAYGEWAAKKLKSKRATLVATSLLGAL
                     IFVDDYFNCLTVGSVMRPVTDKYKVSRAKLAYLIDATAAPVCIIAPISSWAAAVNSYV
                     PAGSAMSGFEMFVRTIPFNLYALLTLYMVFFISLVGFDFGLMKKHERNAANGDLFTSG
                     GEAFQNQETHDPTEGGKYAKGKVIDLIAPMIVMIATAIGAMIWTGYLNGGTNLVEDFA
                     NCSSSESLVFAGLITVGFLLVLYLPRRVIGFKDFMNSVPEGGKLMMPAILILVLAWTL
                     KGMTDALGIGDFVRQAVSLNAGLMRFVPLLIFCIAIFIAFSSGTSWGTFAILVPIVVN
                     MFSETDPNMMIVAVAAVLAGAVCGDHISPISDTTIMSSSGAQSNHINHVQTQMQYAMV
                     VVAACVPGYLIAGFTENWLLTLAASLVILTVMLLWLRKRSLAE"
     gene            complement(1869..2777)
                     /locus_tag="ACZPAX_05415"
     CDS             complement(1869..2777)
                     /locus_tag="ACZPAX_05415"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005933373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HIR1113371.1"
                     /translation="MKELHVKSLLPVRLDKYLMDQYPALGMGRLNKALRENKIKLNGK
                     RQPLSTRVQNGDIIKLFLTDEQLENRPTPAAVFVYEDDDIIIASKPAGIAVDGPDTDT
                     LIRRVQAKLASEGKATHAVLCHRLDTGTSGLVLLAKNNAAEAFLTAAIKARDIEKRYL
                     CVTFGRPNPPAALLRDYLLKDAERGIVRITDTTAGGAKEVITGYETLAVSGRLALLEV
                     ELVTGRTHQIRAHLAHIGCPILGDSKYGSNAANRELRFKYQALCAWELRFPAQISDPR
                     FAHLAGRVFHAEKPWYYQQVVDGTLK"
     gene            complement(2774..4723)
                     /locus_tag="ACZPAX_05420"
     CDS             complement(2774..4723)
                     /locus_tag="ACZPAX_05420"
                     /inference="COORDINATES: protein motif:HMM:NF039722.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6311 domain-containing protein"
                     /protein_id="HIR1113372.1"
                     /translation="MLNHKLSRKAQLFLLGALLGVAIFLLVYGTAPLNVANDAFCRGG
                     YIEKDIQQHYAGWLFYKRSAIGWPLGVTDAVNYPGGVSVAYTDSIPLLAALCRPLAAA
                     LGGTFQYFGWFTLVCFALQGGFGALLCSLFAGGLFSPLAGSLVFAASPVLIERAFRHT
                     SLGAQWLVLAALYCYFVCKRQGLYAPRGLFLLNVLAVGIHPYFLPMTYAVTLALLLEY
                     AVQKRQWLRPAVYLGSNMVCTATLGWALGLLYGTATSGGQALYGYFAMNLNALWNPVG
                     VNGVLYSRLLPAQNQVGGNYDAFAYLGLGVLAALPVALVLARRRLAGYLRRHWTLCFC
                     CVVLTAFAVSNVITANGVTLATLPLPASFIKLFSVFRSGGRLFWPVYYLLVLAAFAGL
                     ARLPQAAVWVAVFAAVQLWDISPALIQRHDAMRDAQTADAFPTEMQSSFWQTASGRYA
                     HLESVEGMQADSLHLALWAADNDMTTNDPFAARYDETALATERKTALDALANGDLQND
                     TLYLFATEGAFLQAVEPVKDAAWCGRVTSTDGTASWYVIAPGLQGKTFDALCTPYDEN
                     YPLRLADYTDALWNRGVLDSTKKTICFMDSHFARAKLDGAAYLCAGGREYPILAVDDH
                     DAGWLMVTLEIDDATILWDEELTTK"
     gene            complement(5006..>5362)
                     /locus_tag="ACZPAX_05425"
     CDS             complement(5006..>5362)
                     /locus_tag="ACZPAX_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004064515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HIR1113373.1"
                     /translation="ITTEIGADIKVLDMPLLDTSTATENLDKRFIADLVLQILSYTAE
                     KERTNIRKRQAQGIEVARAHGVKMGRPTKDYPENWQTVYIKWKQNQILAVDAMRELNL
                     TKPTFYRLVKRYEGKD"
BASE COUNT         1120 a         1564 c         1535 g         1143 t
ORIGIN      
        1 atatcgtaat atcatcgtga ttggcatacc ctataataag gtgattgcat gctgattata
       61 ggggaacgaa ttaaaatgct gcgggagagt cacaaccttt cacaggccgc gctggcaaag
      121 cggctgggca tcacacggtc gatctggggc tgctgtggag cattgccgat accttcaacg
      181 gccttatgag catcccgaac ctcattgcgc tgctcctgct gtccggtacg gtggtcgaac
      241 tgaccaagga ctattttgcg aaagaaaagg tgtaaaaaat gtttgtagga acctggtggt
      301 ctctgatgcc gcctatcatc gccatcctgc tggcgctgct gacgaaagaa gtttacctct
      361 cgctgtttgc gggctgtgcg ctgggggcgc tgctggtggc ggagttccac ccttggctgg
      421 catttgacac gctgttcaac acgatgatca acagcgttga cttctcgatt ctgatgttca
      481 tgatcttgct gggcatggtc gtcatgctga tgcaggaatc cggcggtacg cgcgcctacg
      541 gcgagtgggc ggcaaagaaa ctcaagagca agcgggccac cttggtggca acgtcgctgc
      601 tgggggcgct gatcttcgtg gacgactact ttaactgtct gaccgtcggc tcggtcatgc
      661 gcccggtcac ggacaaatac aaagtttctc gcgcaaagct ggcctacctc atcgatgcta
      721 ccgctgcacc ggtctgcatc attgcgccga tttcctcctg ggcggcagca gtcaactcct
      781 acgtcccggc gggcagcgcc atgtccgggt ttgaaatgtt cgtgcgcacg ataccgttca
      841 acctctatgc gctgctgacg ctctacatgg tgttcttcat ctcgctggtc gggtttgact
      901 tcggcctgat gaaaaagcat gagcgcaacg ccgccaatgg tgacctattc acctcgggcg
      961 gggaggcatt ccaaaaccag gagacacacg accccacaga gggcggcaaa tacgccaagg
     1021 gcaaggtcat tgacctgatt gcgccgatga tcgtgatgat tgcaacggcc atcggggcta
     1081 tgatctggac cggctacctg aacggcggca cgaaccttgt ggaggatttc gccaactgct
     1141 cctcgtcgga gtcgctggtc tttgcgggac ttatcacggt ggggttcctg ctggtgctct
     1201 atctgccgcg ccgggtcatc gggttcaaag atttcatgaa ctctgtgccc gagggcggca
     1261 agctgatgat gcccgctatc ctgattctgg tgctggcttg gacgctgaaa ggcatgactg
     1321 acgcgctggg catcggcgat tttgtacgcc aggccgtcag cctgaacgcc gggctgatgc
     1381 ggtttgtgcc gctgttgatt ttctgcattg ccatcttcat tgcgttttcc tccggcacca
     1441 gctggggcac gtttgccatt ctggtgccca tcgtcgtcaa tatgttcagc gagacagacc
     1501 ccaacatgat gatcgtggcc gtagccgctg tgctggcggg cgctgtctgc ggcgaccata
     1561 tctcgccaat ctccgacacc accatcatgt ccagttccgg cgcacagtcc aaccacatca
     1621 accacgtgca gacccagatg cagtatgcca tggtcgtggt ggctgcctgc gtgcccggct
     1681 acctgattgc gggctttacc gagaactggc tgctcacctt ggccgcgtcc ctcgtcatcc
     1741 tgaccgtgat gcttctctgg ctgcgcaagc gcagtttagc agaataaaaa aagcagtgct
     1801 ttcaaaattg aaggcactgc ttttttattc tgcgtgcccg ggcggattct atatccgccc
     1861 gggccgtctt atttcaacgt tccatccacg acctgctggt aataccacgg cttctcggcg
     1921 tggaacaccc gcccggccag gtgggcaaaa cgcgggtcgg aaatctgtgc agggaagcgc
     1981 agctcccacg cgcagagggc ttgatacttg aaccgcagct cccggttggc ggcgttgctg
     2041 ccgtacttgg aatcgcccaa gatcgggcag ccgatgtgtg cgaggtgcgc ccgaatctgg
     2101 tgggtgcgcc cagtcacaag ctcgacctcc aacagggcca gccgtccgct gacggccagc
     2161 gtctcatacc cggtgatgac ttccttcgcg ccgcctgcgg tcgtgtcggt aatgcgcaca
     2221 atgccgcgct cggcgtcctt caaaaggtaa tcccgcagca gggcggcggg cgggttcggg
     2281 cgaccgaacg tcacgcagag ataccgcttt tcaatgtccc gtgccttgat ggcggcggtc
     2341 aaaaatgcct cggcggcgtt gttttttgcc agcagcacca ggccgctggt gccggtgtcc
     2401 agtcggtggc agagcaccgc gtgggtggcc ttgccctcgg aggccagctt tgcctgcacg
     2461 cggcgaatca gcgtgtcggt gtccggccca tccaccgcaa tgcctgcggg tttgctggca
     2521 atgatgatat cgtcgtcctc atacacaaat acggcggcgg gggtggggcg gttttccagc
     2581 tgctcatcgg tcaaaaatag cttgatgata tcgccgttct gtacccgggt cgaaagcggc
     2641 tgccttttgc cgttcagctt gatcttattc tcgcgcaggg ccttgttcag ccgccccatg
     2701 cctaaggcgg ggtactgatc catcaggtat ttatccagcc gcaccggcag caggcttttc
     2761 acgtgcagtt ctttcatttt gttgtcagct cctcatccca caaaatcgtg gcatcgtcaa
     2821 tttccagcgt aaccatcagc caaccggcgt catggtcgtc cactgccagt atcgggtatt
     2881 cgcggcctcc ggcacacagg tatgcagcac cgtccagctt tgcgcgggca aagtgagaat
     2941 ccataaagca gattgtcttt ttggtggaat ccagcacacc gcggttccac aaagcgtcgg
     3001 tgtaatccgc cagccgcagg ggatagtttt catcgtaggg cgtgcagagc gcatcaaaag
     3061 tcttgccttg cagccccggc gcgataacgt accagctggc cgtgccgtcg gtacttgtca
     3121 cccggccgca ccaggcggca tcttttaccg gctcaacggc ttgcaaaaat gcgccttcgg
     3181 tcgcaaataa atagagagta tcattctgca aatcgccgtt cgccagcgca tccagcgcgg
     3241 ttttccgctc ggtggcaagg gcggtttcat catagcgggc ggcgaagggg tcgttcgtgg
     3301 tcatgtcgtt gtcggcggcc cacagcgcca ggtgcaggct gtcggcctgc atcccttcca
     3361 cggattcaag gtgtgcgtac cgcccggagg ctgtctgcca gaagctgctt tgcatctcgg
     3421 taggaaaagc gtccgccgtc tgcgcgtctc gcatggcatc gtgccgctgg atgagcgcgg
     3481 ggcttatatc ccacagctgc accgctgcaa acacggccac ccagactgcc gcctggggca
     3541 gccgtgccag cccggcgaac gccgccaaaa ccagcaggta atacacaggc cagaacagcc
     3601 gccctccgga gcggaaaacc gaaaacagtt tgataaagga agctggtaac ggcagcgtgg
     3661 ccagcgtaac gccgttggcc gtgatgacgt tgctcacggc aaacgcagtc agcaccacac
     3721 agcaaaagca gagcgtccag tggcggcgca gatacccggc caaccgccgc cgcgccagca
     3781 ccagcgccac cggcagcgcc gccagcacgc ccagccccag gtaggcaaaa gcgtcatagt
     3841 tgccacccac ctggttttgc gcgggcagca gacggctgta caaaacgccg tttacgccca
     3901 ccgggttcca cagcgcgttt aaattcatgg caaaatagcc gtacagcgcc tgcccgccgc
     3961 tggtggccgt gccgtacaaa agccccagtg cccagcccag cgttgccgtg cagaccatat
     4021 tgctgcccag atacacagcg ggccgcagcc attgccgctt ttgcacggcg tattccaaca
     4081 gaagcgccag cgtgacggcg taggtcatgg gcagaaaata ggggtggatg cccacggcta
     4141 aaacattgag caaaaacagc ccccgcggcg catacagccc ctgccgctta cagacaaaat
     4201 agcagtagag cgctgccagc acaagccatt gcgcccccag tgaggtgtgc cggaacgcgc
     4261 gttcgatcaa aacagggctg gcggcaaaga cgagcgaccc cgccagtggg gaaaacaacc
     4321 cgcccgcaaa cagcgaacat agcagcgccc caaaaccgcc ctgcaaggca aagcacacca
     4381 gcgtgaacca gccaaaatac tggaacgtgc cacccagcgc cgccgccagc gggcggcata
     4441 aagccgccag cagggggatg gagtcggtgt aggctacgct gaccccgcca gggtagttga
     4501 cggcatccgt cacacctagg ggccagccga tggcgctgcg cttataaaac agccagcccg
     4561 cgtagtgctg ctggatgtcc ttttcgatat agccgccgcg gcagaatgcg tcgttggcga
     4621 cgttcagcgg cgcggtgcca tacaccagta aaaagatggc cacccccagc agggcaccca
     4681 gcagaaacag ctgcgctttg cgggataatt tatggttcag catatagccc attcctcgca
     4741 ctaaaaaaca aaagtctaac cgaccggacg gaaggttaga ctttacgagg aaatgccgct
     4801 gaccgggatc gaaccggtac ggtatcgcta ccgagggatt ttaagtccca ctaaaagcgg
     4861 tatttctaca tattgagtcc aaacgccttt tggttagcat gttttttcta tatattgtgt
     4921 gtatctaaag taacaggaaa aatattacaa gagattgaag aattgaaatt ttggtagagg
     4981 gattttcaaa taggcataag aatatttaat ctttaccttc atagcgcttc accagtcgat
     5041 aaaatgtagg cttcgtaagg ttcaattctc gcattgcgtc aacagcgaga atctgattct
     5101 gtttccattt gatatacacg gtctgccaat tttcggggta gtcctttgtg gggcggccca
     5161 tcttaacgcc gtgtgcgcgc gcgacctcaa tgccttgcgc ttgacgctta cggatgtttg
     5221 tacgctcttt ctcggcggtg tagctaagaa tctgcaagac caaatctgca atgaagcgtt
     5281 tgtcaaggtt ctctgttgcg gtgctggtgt ccagcagggg catatcgaga actttaatat
     5341 ccgcgccgat ctcggtggtg at
//
LOCUS       DBMDSQ010000116         5361 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_3737_length_5361_cov_1.525475, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000116 DBMDSQ010000000
VERSION     DBMDSQ010000116.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 5361)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5361)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5361
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3737_length_5361_cov_1.525475"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(105..482)
                     /locus_tag="ACZPAX_05430"
     CDS             complement(105..482)
                     /locus_tag="ACZPAX_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003533139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit C"
                     /protein_id="HIR1113374.1"
                     /translation="MKTKFSKLFVTILIVAAVTCCGALGVFAADDAATDTTAATTASE
                     TVNTDNDHNSSLSIGLVAAALATGLAGIGGGMAVASAAPAAIAANSENEKTFGKSLIF
                     VALGESIALYGLVISIMILSKLV"
     gene            complement(635..2551)
                     /locus_tag="ACZPAX_05435"
     CDS             complement(635..2551)
                     /locus_tag="ACZPAX_05435"
                     /inference="COORDINATES: protein motif:HMM:NF013647.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HIR1113375.1"
                     /translation="MVEKMKLVRVQGTMPQLNEFIGSCCMDGRFDLEPATRYMSESLG
                     YGALNEENPHTAIRDQIETMAQEANIELHKGQQHSDPVDAEGRSYLSDLLEQIDELCA
                     ERKILLDQKKLCEDGIEQYSHFTGLKVNVDDLLDCAHVKVRFGFLPTEGYNKLMNNYG
                     DDPYILFTPCNQTKAGYWGVYMTPREKALETDGIFSMLYFEPVHVPGAAGSPAEIIEH
                     FKENLDVVNKSVDDVNGRIATLWQKNAEKVQLLYDTACYYAAVYDLRRMAAVKGQHFF
                     CVGWVPASEVDEVAGKARAVKGLRVTVDGPESAGKMTPPTKLKNPWWSRPFEFFVEMY
                     GLPAYGETDVTGFVAITFTVLFGMMFGDVGQGIVLALFSTFMWKVKHNDLFHLMIPCG
                     ISSTIFGLVYGSLFGYEEALDPLYHAIGMAGKPVSVMDSITGILMVAVYIGIVLVLAA
                     MTLNMYTHARHKEWGEFIFSPNGLVGVVTYLCGVDLASAYMGAVTFMPQVVAIIGLVV
                     GLVLLLFAELLAPMVEGKPWQPAGGMGNYLMQSVFELLETVLSYLSNTISFLRVGAFV
                     IVHASMMMVVFTLAGTPNNIVVVILGNLVVICLEALLSAIQGIRLEFYEMFSRCYKGG
                     GRKFVAFDLKKEKA"
     gene            complement(2695..3753)
                     /locus_tag="ACZPAX_05440"
     CDS             complement(2695..3753)
                     /locus_tag="ACZPAX_05440"
                     /inference="COORDINATES: protein motif:HMM:NF014094.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000220 - vacuolar proton-transporting
                     V-type ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V0D/AC39 family V-type ATPase subunit"
                     /protein_id="HIR1113376.1"
                     /translation="MMKASAASGNAVLAKARALYGRRLRADDYRRLMGCRTMTELAAA
                     LKEYPLYADALAEVNPQYARRAQLENLLRQGLYARYDSLCRYDRSAGSKVYEYFTLCC
                     EVDELTAAMRCLDAGRPGDYLFRLPEFMQQRCCIDLYALAKATSLDGILAAVADTPWE
                     KVLEPMKAAKPDRGLTAQAEPLLQDRRHKALVALAPAKKGTSAAPNLRDLVELECDTS
                     AVSNAARLIRIGAPDSVVRSNARRDCTAMTNDEWGYLLAARDLADFKARFAKTKYGPL
                     LGHHEYSVLKEGFFHYRCDWCKKWLRFSTDPTLVMLCYVWLAQCEVQDLDHIIEGVHY
                     KLPPEEVQSLLVGFDEND"
     gene            complement(3750..4082)
                     /locus_tag="ACZPAX_05445"
     CDS             complement(3750..4082)
                     /locus_tag="ACZPAX_05445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113377.1"
                     /translation="MKDVNDLMQAILEMDAAQRKASEKAKAERTARLAALDEQKQKII
                     AECDAREKTECAAAAKAAADGNAAALAALEQQRRQAAETMTAAAKAKQAQWCAELVQR
                     TLGGEAAQ"
     gene            complement(4312..4785)
                     /locus_tag="ACZPAX_05450"
     CDS             complement(4312..4785)
                     /locus_tag="ACZPAX_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HIR1113378.1"
                     /translation="MPQTKKQEIVYTVMMVCVMVYGMVCYNIALDVGGMQNFIFAAAL
                     SELPIMGVIGFLLDTFLVGPAAMRIAMGWFTPGKDKQVFLVAAISCLSAWMMCPLMSF
                     FATLFFKDSHNANFFAMWVQTTAMNYPTAFLWQLCGAGPLVRFVFGKGLAAMKKQ"
     gene            5121..>5361
                     /locus_tag="ACZPAX_05455"
     CDS             5121..>5361
                     /locus_tag="ACZPAX_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008717854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HIR1113379.1"
                     /translation="MSHSLLHHRGQMSEAFRTVMFLSASGGLQDAYTYIGRGKVFANA
                     QTGNIVLMSQSLFEGDFARFAHYFITVLAFALGVAA"
BASE COUNT         1046 a         1618 c         1572 g         1125 t
ORIGIN      
        1 gccgcaggtc ggcctcgctg cgggctacgg tgccctcaat gcccgccaga cgcaggccgt
       61 tcatggcgtc ggagttgtcg ctgatgagaa agaaccgcat ggccttacac cagcttgctc
      121 aggatcatga tggagatgac caggccgtac agggcgatgg attcacccag ggccacgaag
      181 atcaggctct taccgaaggt cttttcgttc tcgctgttgg cagcgatagc agcaggcgca
      241 gcggaggcaa cggccatgcc gccgccgata ccggccaggc cggttgccaa agcagcagcc
      301 accaggccga tgctcaaaga gctgttgtgg tcgttgtcgg tgttgacagt ctcggacgca
      361 gtcgtggcag cggtggtgtc ggtggcggca tcatcggcgg caaagacgcc cagtgcgccg
      421 cagcaggtca cagcggccac gatcaggatg gtaacgaaca gtttgctgaa cttggttttc
      481 ataatggaac cctccgtaaa tttttattgt atccgcgcct ttttgtcagg cgcttttgta
      541 ggggccgggc atgcccggcc cgcggcttcg attctatcga ccggttgtgg gttggttgcg
      601 ggccggacat gtccggcccc tacctcgcgt cctcttacgc tttctctttt ttcaggtcaa
      661 acgccacgaa cttgcggccg ccgcccttgt agcaacggct gaacatctcg tagaactcca
      721 aacggatgcc ctggatggcg gacagcagcg cttccagaca gataaccacc aggttgccca
      781 ggatgaccac gacgatgttg ttcggcgtac cggccagcgt aaagacgacc atcatcatgc
      841 tggcgtggac aatgacgaac gcgcccacac gcaggaagga aatggtgttg gacaggtagc
      901 tcagcaccgt ttccagcagt tcgaacacgc tctgcatcaa gtagttgccc atgccgcctg
      961 cgggctgcca gggcttgcct tcgaccatcg gggccagcaa ctctgcaaac agcagcagca
     1021 ccaaaccgac caccagaccg atgattgcca cgacctgcgg cataaacgtg accgcgccca
     1081 tgtaggcgct tgccaggtca acaccgcaca ggtaggtgac gacaccaacc aggccgttgg
     1141 gcgagaagat gaactcgccc cactccttgt gacgggcgtg ggtgtacata ttcagcgtca
     1201 tggccgccag caccagcaca atgccgatgt aaacggccac catcaggatg ccggtgatgg
     1261 aatccatgac cgaaacgggc ttgcccgcca tgccaatggc atggtagagc gggtcgaggg
     1321 cctcctcgta gccaaagaga gaaccgtaga ccaggccaaa gatcgtgctc gaaatgccgc
     1381 aggggatcat cagatggaac aggtcattgt gcttgacctt ccacataaag gtgctgaaca
     1441 gcgccagcac aatgccctgc ccgacatcgc caaacatcat gccgaacagc acggtgaatg
     1501 tgatcgctac aaagccggtg acatctgtct cgccgtaggc gggcagaccg tacatttcga
     1561 cgaaaaactc aaaagggcgg ctccaccagg ggtttttcag cttggtgggg ggcgtcatct
     1621 tgccggcgga ttccggccca tcgaccgtga cgcgcagccc cttgacggcg cgggccttac
     1681 ccgcgacctc gtccacctcc gacgccggga cccagccgac gcagaagaag tgttggccct
     1741 tgaccgccgc catccggcgc aggtcgtaca cagcggcgta gtagcaggcc gtgtcgtaca
     1801 acagctgaac cttctcggcg ttcttctgcc acagggtggc aatgcggccg ttgacatcgt
     1861 ccacgctctt gttgacgaca tcaaggtttt ctttaaagtg ctcgatgatc tcggcgggcg
     1921 aaccggccgc gccgggcaca tgcaccggct caaagtacag catcgagaag ataccgtcgg
     1981 tttccagcgc tttctcccgc ggggtcatgt agacgcccca gtaaccggcc ttcgtctggt
     2041 tgcagggggt gaacaggata tacggatcat cgccgtagtt gttcatcagc ttgttgtagc
     2101 cctcggtggg caggaagccg aaacgcacct tgacgtgggc gcagtccagc aggtcatcga
     2161 cgttgacctt caggccggta aagtggctgt actgctcgat gccgtcctcg cagagctttt
     2221 tctggtccag caggatctta cgctcggcgc agagctcgtc gatttgttcg agcagatcgc
     2281 ttaaatagct gcggccctcg gcgtccaccg ggtcgctgtg ctgctgcccc ttgtgcagct
     2341 ctatgttggc ctcctgggcc atggtctcga tctggtcgcg gatcgctgtg tgggggttct
     2401 cttcattcag agcgccgtac cccagcgatt ccgacatata tctggtggca ggttccaggt
     2461 caaagcggcc gtccatgcag cacgagccga taaactcgtt cagctgcggc atcgtgccct
     2521 gtacccgcac cagtttcatc ttttcaacca atcagttcac actccttcaa actgtcttac
     2581 acccgtgcgg tgcggcggcc agaggactgg ccgccctacg gtatcgtacc tcgggtgtag
     2641 ggcggtcagt cccctgaccg ccgcggcgtg ttgccgtcac cgacagtgta aaactcaatc
     2701 gttctcatca aacccgacca gcagcgactg gacttcctcg ggcggcagct tgtaatgcac
     2761 gccctcgatg atgtgatcca gatcctgcac ctcgcactgg gccagccaga cgtaacacag
     2821 cataaccagc gttgggtcgg tggaaaaccg cagccacttt ttgcaccagt cgcagcggta
     2881 gtggaagaag ccttccttca gcacgctgta ctcgtggtgg cccagcagcg ggccgtactt
     2941 tgtcttggcg aaccgcgcct tgaagtcggc caggtcgcgt gcggcgagca ggtaccccca
     3001 ctcgtcgttc gtcatagccg tgcagtcgcg gcgggcgttg gaacgcacca ccgaatccgg
     3061 tgcgccaata cggatgagcc gcgctgcgtt agagacagcg gacgtatcgc attccaactc
     3121 caccaagtcg cgcaggttcg gcgcggcgct cgtgcctttc ttggccgggg ccagcgccac
     3181 aagggctttg tggcggcggt cctgcaaaag cggctcggcc tgggcagtca gaccccggtc
     3241 cggcttggcg gctttcatcg gttccaggac tttttcccac ggcgtgtcgg ccacagcggc
     3301 caggatgccg tccaggctcg tggctttagc cagcgcgtag agatcgatgc agcagcgctg
     3361 ctgcatgaac tccggcaggc ggaagaggta atcgccaggg cggcctgcat ccaggcagcg
     3421 catggcggcg gtcagctcat cgacctcgca gcaaagggtg aagtactcgt acaccttgct
     3481 gcccgcgctg cggtcatagc ggcaaaggct gtcgtaccgg gcgtacaggc cctggcgtag
     3541 caggttttcc agctgggcgc ggcgggcgta ctgggggttg acctcggcca gggcgtcggc
     3601 gtacagcggg tactccttca aggccgcggc aagctcggtc atcgtgcggc agcccatcag
     3661 gcggcggtaa tcgtcggcac gcagcctgcg gccatacagg gcgcgggcct tggccagtac
     3721 ggcgttgccg gaagccgcac tcgccttcat cattgtgcgg cctccccgcc cagggtgcgc
     3781 tgtacaagct cggcacacca ttgtgcttgt ttcgcctttg cggcggcggt catggtttcg
     3841 gcagcctgcc ggcgctgctg ctccagtgcc gccagcgcgg cggcgttacc gtcggccgcg
     3901 gccttggctg cggctgcaca ctcggttttt tcgcgggcgt cacactccgc gatgattttc
     3961 tgtttctgct cgtccagggc cgccaaccgg gcggtacgct cggccttggc cttctcgctg
     4021 gccttgcgct gggcggcgtc catctccaaa atggcctgca tcaggtcgtt gacatctttc
     4081 atgcaatgca cctcactctt tgggagcttt acctgaacca atcagcgttt cccttccctt
     4141 gcattctctg cacccggaag tagttcctat tatgtaaata ggtagaccaa ctttttcagg
     4201 ttcagccgta attatagcac tatgaccaaa tatggcaagt ggtttttgtg cagttttatt
     4261 tgtacaaaaa tgggtttgtg tttttcctgt cgatgttttg cgaaatcgat ctcactgctt
     4321 tttcatggcg gccagccctt tgccgaacac aaaccgcacc agcgggcctg cgccgcacag
     4381 ctgccacagg aacgcggtgg gatagttcat cgccgtggtc tgcacccaca tggcaaaaaa
     4441 gtttgcgttg tggctgtctt tgaagaacag cgtggcgaaa aagctcatca acgggcacat
     4501 catccaggcc gacaggcagg agatggccgc taccagaaac acctgcttat ccttgccggg
     4561 ggtgaaccaa cccatggcga tgcgcatggc cgcggggccg accaggaacg tatccagcag
     4621 gaagccgata acgcccataa tgggcagctc gctgagggca gcggcgaaga tgaaattctg
     4681 catcccaccc acatccagtg cgatattgta gcagaccatt ccgtagacca tgacacagac
     4741 catcatgacg gtgtagacga tttcctgttt ctttgtttgc ggcattgtaa ttactcctca
     4801 tatgtacgtt attttctcca aaacaaaaac agccgtaggg tgtcgctcaa ctcgttgcga
     4861 caccctgcgg ctgttgtctc actgcgttgg aaagtggagc cagtataccg caaattttcc
     4921 gcttatgtaa tacccctgca caaatttctg cactttgtat aaaatatttc tccgttttgc
     4981 cgcgtcactt tggtacaaaa cgccgcatgt gtcgccgtaa actttacagc ggcggcgcgg
     5041 tgcgctataa tgggcaatga ttgcaggggc ggccgttggc cgcccctgca cgcgcaatca
     5101 catagagggg tcgaaaaatc atgtcacatt cactattgca tcaccgcggg cagatgtccg
     5161 aggcattccg cacggtcatg tttctgtccg catcgggcgg gttacaggac gcctacacct
     5221 acatcgggcg cggcaaggtg tttgccaacg cccagaccgg caacatcgtc ctgatgagcc
     5281 aaagcctgtt cgagggagat tttgcacggt tcgcccacta tttcatcacg gtactggcct
     5341 ttgcgctggg tgtggcggcg g
//
LOCUS       DBMDSQ010000117         5355 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_3741_length_5355_cov_2.283974, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000117 DBMDSQ010000000
VERSION     DBMDSQ010000117.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 5355)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5355)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5355
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3741_length_5355_cov_2.283974"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..908)
                     /gene="argS"
                     /locus_tag="ACZPAX_05460"
     CDS             complement(<1..908)
                     /gene="argS"
                     /locus_tag="ACZPAX_05460"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase domain-containing protein"
                     /protein_id="HIR1113380.1"
                     /translation="MDYKNYNPRAAALAQARALLTDAVKAAVADGALPEAALPDFIVE
                     IPADVKNGDIASNVAMAGARAFHKAPRQIAEAITAKLQLGGSLFDRFEVAGPGFINLF
                     LGQDWFTSVVRAAVANPEYGRTDVGAGKRYNVEFVSANPTGPMHMGNARGGALGDGLA
                     ACLDWAGYDVTREFYINDAGNQIEKFGKSLAIRYLQLYKGEDACPLPEECYQGADIIA
                     RAKEFAAVHGDAYVNKDFDELKKAIVADALPKNIAGLQRDLGKYRIEYDVWFHESDLH
                     NSGAVKAVVDKLLETGACYKAEDGAI"
     gene            complement(1031..1468)
                     /locus_tag="ACZPAX_05465"
     CDS             complement(1031..1468)
                     /locus_tag="ACZPAX_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwiB family protein"
                     /protein_id="HIR1113381.1"
                     /translation="MEEKYLITIKGTMEQRGDTDTVELMTRGSLVHKDGAYYIVYKET
                     EATGYEGCTTTVKVADDARKVSMLRFGKQTSQLVIEKGTRHLCHYETGYGAISLGVSA
                     DVIEHALDENGGKLQFSYTLDSGAESFISRNLVDITVDKLPES"
     gene            complement(1679..2494)
                     /gene="murI"
                     /locus_tag="ACZPAX_05470"
     CDS             complement(1679..2494)
                     /gene="murI"
                     /locus_tag="ACZPAX_05470"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HIR1113382.1"
                     /translation="MDPRPIGVFDSGLGGLTAVRQLRRVLPGEDIVYFGDTGRVPYGS
                     RGRDIIVQYARQDIRFLLQQDVKFIIAACGTVSSTYPPEEAAKLPVPFTGVVGATARA
                     AVDATRNKKVGIIGTAATVRSGSYAAVIRDMMPEVEIFARACPMFVPLVENGYFNDGN
                     PVTKLIIAEYLQELKDAGVDTLILGCTHYPLLKKMIGDFMGGDVHLVDSGKVTAQAAA
                     AALDDLGLLNGKTSGGTAKYFVSDTPDNFDELAHTFLGEYAGGTVERIAIETY"
     gene            complement(2494..3603)
                     /locus_tag="ACZPAX_05475"
     CDS             complement(2494..3603)
                     /locus_tag="ACZPAX_05475"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HIR1113383.1"
                     /translation="MNSNSKLRVALFFGGVSSEHEVSCVSASAWLRALGQSPCAEQYE
                     VFPVGITKTGRWLACHPTPEAMADGSWEQGSCTPCVLSPDRADHGIWLLKDGKAELVR
                     IDICAPVMHGKNGEDGTIQGLFEMARIPYVGCGVLGSAVCMDKAVANALMDAAGVPHC
                     KWVAASRADLALNGPVVLDAVEAKLGYPIFVKPANAGSSVGISKAADRAALEQAVEIA
                     LREDDKVVFEEFIDAQEVECAAIGNPDDPASVMTTRPGEILAGAEFYTYDDKYKNGVS
                     QTVIPAHLSEEKLDEVKAEARKAYLALNCAGLSRCDFFVERGTGRVLCNELNTLPGFT
                     PISMYPKLMEHEGLSYAALVDKLLQLALHRRKGAY"
     gene            complement(3920..4240)
                     /locus_tag="ACZPAX_05480"
     CDS             complement(3920..4240)
                     /locus_tag="ACZPAX_05480"
                     /inference="COORDINATES: protein motif:HMM:NF024835.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1113384.1"
                     /translation="MEFNERLRYLIDCEEIKIKDLAPKLCLSASTLSNYTQGIREPDY
                     DTLRRIADYFGVSIDYLLGHKTPAEDDERQLLALFRSFTPSQRRLLLDQAELLTHYKI
                     KLDD"
     gene            4401..4919
                     /locus_tag="ACZPAX_05485"
     CDS             4401..4919
                     /locus_tag="ACZPAX_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002608801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HIR1113385.1"
                     /translation="MEENKVFKKLKDKKGFTLVELIVVLVILAILAALLVPALTGYID
                     KARNKQIIAETRSAVMAAQTLVDEAYANTSKAVEDTTTAATDKVVVGDKDVTYEAIAK
                     LAELDQTKISGVMITKDGKLKQLIYKDGKTCYYSTSTPTGVGTAIETNGNYYVYNSAS
                     NAAGGNTPGNNG"
BASE COUNT         1050 a         1618 c         1598 g         1089 t
ORIGIN      
        1 atgatggcgc cgtcctcggc cttgtagcag gcgccggtct ccagcagctt gtccaccacg
       61 gccttgactg cgccggagtt gtgcaggtcg ctctcatgga accagacatc gtactcgatg
      121 cggtacttgc cgaggtcgcg ctgcagaccg gcgatgttct tgggcagggc atcggccaca
      181 atggcttttt tcagctcgtc aaagtccttg ttgacgtagg cgtcgccgtg gacggcggca
      241 aactccttgg cgcgggcgat gatatccgca ccctggtagc actcctcggg cagggggcag
      301 gcgtcctcgc ccttgtagag ttgcaaatag cggatggcaa ggctcttgcc gaacttctcg
      361 atctggttgc ccgcatcgtt gatgtagaac tcgcgggtca cgtcgtaacc ggcccagtcc
      421 aggcaggcgg ccaggccgtc gcccagggca ccgccgcggg cgttgcccat gtgcatgggg
      481 ccggtggggt tggccgaaac gaactcgacg ttgtagcgct tgcctgcgcc aacgtcggtg
      541 cggccgtact cggggttggc cacggcggca cgcacaacgc tggtgaacca gtcctggcca
      601 aggaacaggt tgatgaagcc ggggcctgcg acctcgaagc ggtcaaacag gctgccgccc
      661 agctgcaatt ttgcggtgat ggcctcggca atctggcgcg gggccttgtg gaacgcgcgg
      721 gcgccggcca tggcaacatt ggatgcgata tcgccgtttt tgacgtcggc cgggatctcg
      781 acgatgaagt cgggcagcgc agcctcgggc agggcaccgt cagcgacggc tgccttgacg
      841 gcatcggtca gcagcgcacg cgcctgtgcc agcgccgccg cacgggggtt gtagttttta
      901 taatccatga tgaacctcct gtgggggatt tttctatgat aacgccgcag gggccgggca
      961 ggcccggccc gcggctttgc ctttacaaag tgctttccgg taggctgaga gccgtagggg
     1021 cagcgcggtt ttaagattct ggcagcttat cgaccgtaat gtcaacaaga ttccggctga
     1081 tgaaactctc ggcaccggaa tccagcgtgt agctgaattg cagcttgccg ccgttttcgt
     1141 ccagcgcgtg ctcgatgacg tcagcggaca cgcccagact gatggcaccg taccccgtct
     1201 cgtaatggca gaggtggcgg gtgcccttct cgatgacgag ctgactcgtc tgcttgccga
     1261 accgcagcat cgagactttg cgcgcgtcgt cggccacctt taccgttgtc gtgcagccct
     1321 cgtagccggt ggcctcggtc tctttgtata caatatagta agcgccatcc ttgtgtacca
     1381 gtgagccgcg cgtcatcagc tccacggtgt ccgtgtcgcc gcgctgctcc atcgtgccct
     1441 tgatggtgat gagatatttt tcttccatgg tgtacctcta ctaaaagcct tccccctcgg
     1501 ggacggactc ccccgatacg ggggaggtgg cgcgcagcgc cagaaggggt gcggtggccc
     1561 gcagggccgg atgagggcag accttccgtc gttgcccggg aatgggcggc cacggcaacc
     1621 cgcccctcat cagtcacctt cggtgacagc ttcccccgag ggggaagccc tttttcccct
     1681 agtacgtctc aatcgcaatt ctttccaccg ttccgccggc gtattcgcct aagaacgtgt
     1741 gggccagctc gtcgaaattg tccggtgtgt cgctgacaaa atacttcgcc gtgccgccgc
     1801 tggtcttgcc gttcagcagc cccaggtcgt ccagcgcggc cgcggcggcc tgcgccgtca
     1861 ccttgccgga atccaccaga tgcacgtcgc cgcccataaa atcgccgatc atctttttca
     1921 gcagcggata gtgcgtgcag cccaaaatca gcgtgtcaac gcccgcgtcc ttcagctcct
     1981 gcagatactc cgcaatgatg agctttgtga ccgggttgcc gtcgttgaaa tagccgtttt
     2041 ccaccagcgg cacgaacatc gggcaggccc gggcgaaaat ctccacctcg ggcatcatgt
     2101 cgcggatgac cgccgcatag gacccgctgc gcaccgtcgc cgccgtgccg atgataccca
     2161 cttttttgtt gcgggtcgca tccactgccg cgcgggccgt cgcgcccacc acgccggtga
     2221 acggcacggg cagtttggcg gcttcctcag gcggataggt cgaggacacc gtaccgcagg
     2281 cagcaatgat aaattttacg tcctgctgca ataaaaagcg gatatcctgc cgcgcgtact
     2341 gcacaatgat gtcccgcccg cggctgccgt agggcacgcg gccggtgtcg ccaaagtaga
     2401 cgatatcctc gccgggcagc acgcggcgca gctggcgcac ggcggtcagc ccgcccaggc
     2461 cgctgtcaaa aacgccgatg gggcgtgggt ccatcagtag gcccccttcc ggcggtgcag
     2521 ggccagctgc agcagcttat cgaccagtgc cgcatagctc agcccctcgt gctccatcag
     2581 cttggggtac atactgatgg gcgtaaagcc gggcagggtg ttcagctcgt tgcacaaaac
     2641 gcgcccggtg ccgcgctcca caaagaagtc gcagcgggac aggcccgcgc agttcagcgc
     2701 cagataggcc ttgcgggcct cggccttcac ctcgtcgagc ttttcctcgc tcaaatgggc
     2761 ggggatgacg gtctgggaca cgccgttttt gtacttgtcg tcgtaggtgt agaactcggc
     2821 cccggccaga atctcgcccg ggcgggtcgt catgacgctg gcggggtcgt cagggttgcc
     2881 gatggcggca cactcgacct cctgcgcgtc gatgaactcc tcaaacacga ccttgtcgtc
     2941 ctcgcgcagg gcaatctcca cggcctgctc cagcgcggcg cggtccgcag ccttgctgat
     3001 gcccacgctg gagccggcgt tggccggctt gacaaagatg gggtagccca gctttgcctc
     3061 cacggcgtcc agcaccacgg ggccgttcag cgcaaggtcg gcgcggctgg cggccaccca
     3121 cttgcagtgg ggcacgcccg cagcgtccat cagggcgttg gcaacggctt tgtccatgca
     3181 gacggcgctg cccagcacgc cgcagcccac gtaggggatg cgggccatct caaacaggcc
     3241 ctgaatggtg ccgtcctcgc cgtttttgcc gtgcatgacc ggggcgcaga tgtcaatgcg
     3301 gacaagctcg gccttgccgt ctttcagcag ccagataccg tgatccgcgc ggtccgggct
     3361 gagcacacag ggcgtgcagc tgccctgctc ccagctgccg tcggccatgg cctcgggcgt
     3421 ggggtggcag gccagccagc ggccggtctt ggtgatgccc accgggaaca cctcatattg
     3481 ttctgcgcag gggctttggc ccagggcgcg cagccaggcc gaggccgaca cgcagctgac
     3541 ctcatgctcg ctggagacgc cgccgaagaa gagcgcaacg cgcagcttgg aattactatt
     3601 catcaaaaag actccctttg taaaattgcg cggccccttg gggcacacta caagtcttca
     3661 gcttataatc ataacatatt atgcggccgt tggctagatt atttttacga ttttttaggc
     3721 gaatatatcg tagggggaga gatttatccc gcccggcgga cttcctaggc agatgtacgg
     3781 ccaaaacgcc gccgccctgt agggaccgca catgtgcggc ccgcagcttc atgtagggaa
     3841 ccctgcgggg cgtcgaggac gccgccccct acaaccgaag gccgctataa aacaaaaaac
     3901 gacccgccat cccggcaggt cagtcgtcca gcttgatttt gtagtgggtc agcagctcgg
     3961 cctgatccag cagcagccgc cgctggctgg gggtaaagct gcggaacagc gccagtagct
     4021 gacgctcatc gtcctcggcc ggggtcttgt ggccaagcag atagtcgatg ctgaccccaa
     4081 aataatccgc aatgcgccgc agggtgtcgt agtcaggttc acggatgccc tgtgtatagt
     4141 tactgagcgt cgaggcactc aagcacaatt tgggtgcaag atctttaatt ttgatttctt
     4201 cgcagtcaat caaatagcgt aaacgttcgt taaattccat aaatttgcct ttttccataa
     4261 aactttggtt tgagaataac agaaaaacac aaaacgagtt gcaaaagcac gacttggctt
     4321 gacaaagcac aaaatgtgtt ataaaatgac aatatgaaat gccgacatgg cgctgaaatg
     4381 ttggtgtaaa agagaaaaat ttggaggaaa acaaggtgtt caaaaaactg aaagacaaaa
     4441 agggtttcac cctcgtcgag ctgatcgtcg tcctggtcat cctggcaatt ttggcggcgc
     4501 tgctggttcc cgcgttgacg gggtatattg ataaggctcg caacaagcag attatcgctg
     4561 agacgcgctc cgctgttatg gcggcacaga cgttggttga tgaggcttac gctaatacgt
     4621 cgaaggctgt tgaggacacg acgactgctg caactgacaa ggttgttgtt ggcgataagg
     4681 atgttactta cgaagcaatt gctaaactgg cagaattgga tcagaccaag atttctggcg
     4741 ttatgattac caaggacggt aaacttaagc agttgattta caaagatgga aagacttgct
     4801 attacagcac ttctactcct actggcgtag gtacggcaat tgagactaat ggtaactact
     4861 atgtctacaa cagtgcttca aatgctgccg gtggtaacac cccgggcaac aacggctaat
     4921 tcctttatct aaatgttctc ccaccacctc ccgtggtggg atttttcata ccctctacct
     4981 tataataccc gctctgtagg ggccgggcat gcccggcccg cagccttgcc atagccgcgc
     5041 ggccccgggt cagccgtagg ggagggattc atccctcccg cagaaaagcc tcccttgtca
     5101 aagggaggtg gacgccgaag gcggccggag ggattctgac gggccttgcc ctgcacgccg
     5161 tcacccccgc agccgggcgg gaatccctcc gcctcgcttg cgctcggcac ctccctttga
     5221 caagggaggc ctttcgacaa tccgccccta caacctccga aaggcggtta ccatgcctca
     5281 gacaccaaag ctttctccac aacaaaaggt cgcgcttgtg gaaaaaataa tatccggcga
     5341 gaccagccgc caagc
//
LOCUS       DBMDSQ010000118         5350 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_3746_length_5350_cov_2.004572, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000118 DBMDSQ010000000
VERSION     DBMDSQ010000118.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 5350)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5350)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5350
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3746_length_5350_cov_2.004572"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1698
                     /locus_tag="ACZPAX_05490"
     CDS             <1..1698
                     /locus_tag="ACZPAX_05490"
                     /inference="COORDINATES: protein motif:HMM:NF042863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HIR1113386.1"
                     /translation="AQGVEGVDHPVNAWVRAQAQEFLDVCDGKDKKYCINEKKLRKIG
                     KLMKLMQMPSGLVAGKSIYEATSGYQWLFYAAVLCVVYRNDLKRRRYETAVLEICRKN
                     FKTFTVAVCFLLLMLTEPRFAKLFSVAPDGALSREVQKAIKEILRSSPALMPPDDPNK
                     YFKILRDKITFKLKDTEYTPLNYSNSRLDGRLPNVFLVDEAGALPNPYAIEAMRSGQL
                     TILNKLGCIISTKYPRSDNPFEDEVAYAKNVLEGRVQNEALFALLFEPEESIQKQWAT
                     NNNVLAQGNPAALTLPTVWEDLLNRRQKAVEQESTRENFLCKHCNIIYQGAGTESYIP
                     IDVVQACRVDDIQWAGCEVYLGVDLSMSNDNCAVAMVGLIGDRIAARVMGFIPADKVD
                     EKTSLEKFDYRMAVRNGECIACGDRTVDYGTIENWVFDLEETYGVRVQGVAYDRYNAL
                     SSAQKWERGRYREEGKPHDGYETVQVRQHSDTLHPATKFLQEICESGRFAYQKNKLLE
                     VNFENARCTYDTNMNRYVTKKRSLGKVDMVVALINAIYLLQQEVLLDEDDGWVCQMV"
     gene            1720..3063
                     /locus_tag="ACZPAX_05495"
     CDS             1720..3063
                     /locus_tag="ACZPAX_05495"
                     /inference="COORDINATES: protein motif:HMM:TIGR01537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HIR1113387.1"
                     /translation="MGLKAAIKALREPENNDAVDNEAFADFLAALNGNGKVTREIARD
                     VPGCSGAVEFIAAQAAGVPFRLYEMTDTGPREVTDDVRTHILNGDTNDLMDATSMKVA
                     MYMDYLYEGNGYAYISRAGNRVDGLYYVEQRSVAVNKGTHPIYKQARFLVQGQEYYEF
                     DFLKLCRHTQDGVTGHGLLEEQGQILALAWAGMVLQTNLMKSGGNKRGFLSSEKRLSQ
                     QSLDALKESFERMYSNTTSGAVVLNSGVTFKEASNTSVELQLNETNQAVQNTICTAIA
                     LPPEAINGKGTEETLEIAVQQAVIPLLTKMEAALNRDLLLEEEKGRMYWKADTFELLK
                     GNMAKRYEAYKRATEGGWIDKNEIRRREQMEEKKGLDIVGLGLGEVLLNTKTGQYFTP
                     NTKTVTDLNGTSRVVDGAEGVGGNGKAAAPPGEGGKRGVGDAAPYNEGLKGGEGK"
     gene            3060..3743
                     /locus_tag="ACZPAX_05500"
     CDS             3060..3743
                     /locus_tag="ACZPAX_05500"
                     /inference="COORDINATES: protein motif:HMM:NF016471.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HIR1113388.1"
                     /translation="MKAKIKGDSVVVSGYVNAVERNSVPLRYMRRGNEHTGKKYVERV
                     QAGAFDRAIKKAASVPLRFNHGWDADLPGDIGSTAEGSLKLREDNIGLYAEATITEPK
                     VVEKARRGELTGWSFLFRCPAGGDTWEEPKDGVPLPCRTLKDIELLDVSVLDRKPAYP
                     ANSLHVESRAADDDGDDTGDVLEARSTDEVVETVEEKPPAAQTEEEKRAALRMERNRR
                     ALRIMKLEE"
     gene            3748..5040
                     /locus_tag="ACZPAX_05505"
     CDS             3748..5040
                     /locus_tag="ACZPAX_05505"
                     /inference="COORDINATES: protein motif:HMM:TIGR01554.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HIR1113389.1"
                     /translation="MKLKKLIEQRNAKLTRMNELMNQAENEARALTEDEAKEFDTLES
                     EARALADTIHRNEQLEGAGLNDRNVKKNAPAADDSDIDAAEARAFADYLRGVSLNGTS
                     SAENEARAANMTFGNNGAIVPATIANKIITQVKNISPIYRMASHYNVPGTLTIPYVDT
                     SETDITVGYQDEFVELTSSGHKYGSVSLTGFLFGALTLVSKKLINNAQFDVVPLVIDH
                     MAENIAAWVDKEHLNGTTDKITGVLPGVKQIITATKMTAITADELIDVQEEVPDVYQA
                     NACWIMNKKTRAMVRKLKDGDGNYLLTRDFSADGVNWTLLGKPVYVSDQMPKAEKEKI
                     SVLYGDLSGYAIKESESLEIQVLVEKYATQHAVGVVAWGEQDGKVENAQKLAVLKMPA
                     ADTQALDDEDSPAVVDAAESTDTETVSTQAAKSKKAVK"
     gene            5063..>5350
                     /locus_tag="ACZPAX_05510"
     CDS             5063..>5350
                     /locus_tag="ACZPAX_05510"
                     /inference="COORDINATES: protein motif:HMM:TIGR01560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HIR1113390.1"
                     /translation="MKLLEVDTATAASYCNVPDYDTDGIKVLETIVMPAAKARLESYT
                     GMKAEALNEYEDVTVAYLALCSFLYDNRSMVVENDKENAVLNLLRSYPNVIL"
BASE COUNT         1331 a         1436 c         1741 g          842 t
ORIGIN      
        1 gcccagggcg tggagggagt cgaccaccct gtcaacgcct gggtgcgtgc ccaggcgcaa
       61 gagttcctgg acgtgtgcga cggcaaagac aaaaagtatt gcatcaacga aaaaaagctg
      121 cgaaaaatcg gcaagctgat gaagctgatg cagatgccca gcggccttgt ggccggtaag
      181 agcatctacg aagcgaccag cggctaccag tggctttttt acgcggcggt gctgtgcgtt
      241 gtgtaccgca acgacctgaa gcggcggcgg tacgaaacgg cggtgctgga aatctgccgc
      301 aagaatttca agacgttcac ggtggcggtg tgctttttgc tgctgatgct gacggagccg
      361 cgctttgcaa agctgttcag cgttgcgccg gacggtgcat tgagccgcga ggtgcagaag
      421 gccatcaagg aaattttgcg gtcctccccc gccctgatgc cgccggacga cccgaacaaa
      481 tacttcaaga tactgcgcga caaaatcacg ttcaagctga aggacaccga gtacacgccg
      541 ctgaactact ccaacagccg cctggatgga cgactgccga acgtgttctt ggtggacgag
      601 gcgggggcac tgccgaaccc ctacgccata gaggctatgc gaagcggcca gctgaccatt
      661 ttgaacaagc tgggctgcat tatcagcaca aagtatccac gcagcgacaa cccttttgaa
      721 gatgaggtgg cctatgccaa gaacgtgctg gaaggccgtg tgcagaacga agcgctgttt
      781 gcgctgctgt ttgagccgga ggaaagcatt cagaagcagt gggcgaccaa caacaacgta
      841 cttgcccagg gcaacccggc ggcgctgacg ctgcccaccg tgtgggagga tttgttgaac
      901 cggcggcaga aagccgtgga acaggaaagc acccgcgaaa actttttgtg caagcactgc
      961 aacatcatct accagggcgc gggcacggag agctatattc caatcgatgt ggtgcaggcc
     1021 tgccgcgtgg atgatatcca gtgggcaggg tgtgaagtgt atctgggcgt tgacctgtcc
     1081 atgagcaacg acaactgcgc cgttgccatg gtggggttga tcggcgaccg cattgccgcc
     1141 cgcgtgatgg gatttatccc ggcggataag gtggatgaaa aaacaagcct ggaaaagttt
     1201 gattaccgta tggccgtgcg caacggcgaa tgtattgcgt gcggggaccg caccgtggac
     1261 tacggcacaa tcgaaaactg ggtgttcgac ctggaagaaa cctacggcgt gcgggtgcag
     1321 ggcgtggcct atgaccgata caacgcgctt tccagcgccc agaagtggga gcgcgggcgc
     1381 taccgagagg aaggcaagcc gcacgacgga tacgagacgg tgcaggtgcg gcagcacagc
     1441 gacaccctgc acccggccac aaaatttttg caggaaattt gcgagagcgg gcggtttgcc
     1501 taccagaaaa acaaactgct ggaagtgaat tttgagaatg cccgatgcac ctacgacacg
     1561 aacatgaacc gttatgtgac caaaaagcga agcctgggca aggtggacat ggtagtggcg
     1621 ctgataaacg ccatctacct gctgcaacag gaagttttgc tggatgaaga tgacggctgg
     1681 gtctgccaga tggtgtaacg gaaagggggt gaaaatgcta tgggattgaa agcggcaatc
     1741 aaggcactgc gggagccgga gaacaacgat gcggttgaca atgaagcgtt tgctgacttt
     1801 ttggcggcgc tgaacggcaa cggaaaggtg acgcgggaaa ttgcccggga tgtgcccgga
     1861 tgcagcggcg cagtggaatt tatcgccgcg caggcggcgg gcgtaccgtt ccgcctgtac
     1921 gagatgacgg acaccggccc ccgggaagtg accgatgatg tgcgcaccca cattttgaac
     1981 ggtgatacca atgacctgat ggacgccacc agcatgaagg tagccatgta catggactac
     2041 ctgtacgagg gcaacggcta tgcctacata agccgggccg ggaaccgggt ggacggcctg
     2101 tactacgtgg agcagcgcag cgttgccgtg aacaagggca cgcaccccat ctacaaacag
     2161 gcgcggtttt tggtgcaggg ccaggagtac tacgagttcg actttttgaa gctgtgccgc
     2221 cacacccagg acggcgtgac cgggcacggc ctgctggaag aacagggcca gattttggcg
     2281 ctggcctggg caggaatggt gctgcaaacc aacctgatga aatccggcgg caacaagcgc
     2341 ggctttttga gcagcgaaaa gaggttgagc cagcaatcgc ttgacgcgct gaaggaatcg
     2401 tttgagcgga tgtattccaa cacaaccagc ggcgcggtgg tgctgaacag cggcgtaacg
     2461 ttcaaagaag cgagcaatac cagcgtggaa ttgcagctga acgagacgaa ccaggccgtg
     2521 cagaacacca tttgcaccgc cattgccctg ccgcccgagg ccatcaacgg caagggcacg
     2581 gaagaaacgc tggaaattgc ggtgcagcag gcggtgatac cgctgcttac caagatggaa
     2641 gcggcgctga accgtgacct gctgctggaa gaagaaaaag gccggatgta ctggaaagcc
     2701 gatacctttg aacttttgaa gggcaacatg gcaaaacggt acgaggccta caagcgagcc
     2761 accgaaggcg gctggatcga taaaaacgag atacgccgcc gcgagcagat ggaagaaaag
     2821 aagggcctgg atattgtggg cctgggcctg ggcgaggtgc tgttgaacac caagacgggc
     2881 cagtacttta cgccgaacac caagaccgtt acggatttga acggaacgag ccgggttgtg
     2941 gacggtgcgg aaggtgtggg cggcaacggc aaggccgccg cgccgccggg cgaaggcggc
     3001 aagcggggcg tcggggacgc cgccccctac aacgaaggac tgaaaggggg tgaaggaaaa
     3061 tgaaagcaaa aatcaagggt gacagtgtgg tcgtgagcgg ctatgtgaac gcggtggagc
     3121 gcaacagcgt accgctgcgg tatatgcgcc gcggcaacga gcacaccggc aagaaatacg
     3181 tggagcgcgt gcaggccggg gcgtttgacc gcgccatcaa gaaagcggcc agcgtgccgc
     3241 tgcggttcaa ccacggatgg gatgctgacc tgcccggaga tatcggcagc acggcggaag
     3301 gcagcctgaa actgcgtgag gacaacatcg gcctgtatgc cgaggcgacc atcacggaac
     3361 cgaaggtggt ggaaaaggca cgccggggcg agctgaccgg gtggagcttt ttgttccgct
     3421 gcccggcggg cggcgatacc tgggaagagc cgaaagacgg tgttcccctg ccctgccgca
     3481 cgctgaagga cattgaactg ctggacgtaa gcgtgctgga ccgcaagcct gcctaccctg
     3541 ccaatagcct gcacgtggaa agccgcgccg ccgacgatga cggcgacgac accggggacg
     3601 tgctggaagc cagaagtacg gatgaggtgg tggagacggt ggaagaaaag ccgcccgccg
     3661 cccagaccga agaagaaaaa cgcgcggcgc tgcgcatgga gcgcaaccgc cgggcattgc
     3721 gaattatgaa attggaggaa tgaacctatg aaactgaaaa agctgatcga gcagcgcaac
     3781 gcgaagctga cccgcatgaa cgagctgatg aaccaggccg agaacgaggc ccgcgccctg
     3841 accgaggacg aggccaaaga gtttgacacc ctggaatccg aggcccgcgc cctggccgac
     3901 accatccacc gcaatgagca gctggaaggt gcaggcctga acgaccgcaa cgtgaagaag
     3961 aacgcccccg ccgccgatga ttccgacatt gatgccgccg aggcacgcgc ttttgccgac
     4021 tacctgcgcg gcgtgagcct gaacggcacg agcagcgccg agaacgaggc ccgcgccgcc
     4081 aacatgacgt ttggcaacaa cggcgctatc gtgcccgcga ccatcgcaaa caagatcatc
     4141 acccaggtga agaatatcag ccccatctac cgcatggcga gccactacaa cgtgcccggc
     4201 acgctgacca tcccctacgt ggataccagc gaaacggata tcacggtcgg ctaccaggat
     4261 gagtttgtgg agctgacgtc cagcggccac aagtacggca gcgtgagcct gaccgggttc
     4321 ctgttcggtg cgctgacgct ggtgagcaag aagctcatca acaacgcgca gttcgacgtt
     4381 gtgccgctgg tcattgacca catggccgag aacattgccg cgtgggtgga caaggaacat
     4441 ctgaacggca cgaccgacaa gatcaccggc gttcttcccg gcgtgaagca gatcatcacc
     4501 gccaccaaga tgaccgccat cacggcggat gagttgatcg acgtgcagga ggaagttccc
     4561 gacgtctacc aggcgaacgc ctgctggatc atgaacaaga aaacccgcgc catggtccgc
     4621 aagctgaagg atggcgacgg caactacctg ttgacccgcg atttcagcgc ggacggcgtg
     4681 aactggacgt tgctgggcaa gccggtctat gtcagcgacc agatgccgaa ggcagagaag
     4741 gagaagattt ccgtgctgta cggcgacctg agcggctacg ccatcaagga gagtgagagc
     4801 ctggaaattc aggtgcttgt ggagaagtac gctacccagc acgccgttgg cgttgtggcc
     4861 tggggcgagc aggacggcaa ggtggaaaac gcccagaaac tggcagtgct gaagatgccc
     4921 gccgccgaca cccaggcgct ggacgacgag gacagcccgg cggtagttga tgccgccgag
     4981 agcacagaca ccgagacggt gagcacccag gcggccaaga gcaaaaaggc ggtaaagtaa
     5041 accgcccgga aaggggtaca agatgaagct gctggaagta gacaccgcca cggcggcgag
     5101 ttactgcaat gtgccggact atgacacgga cggtataaag gtgctggaaa ccatcgtaat
     5161 gcccgccgcg aaagcccgct tggaaagcta taccggcatg aaagccgagg cgctgaacga
     5221 atacgaggat gtgacggtgg cctacctggc actgtgcagc tttttgtacg acaaccgcag
     5281 tatggtggtg gaaaacgaca aggaaaacgc agtgctgaac ctgctgcgca gctaccccaa
     5341 tgttatcctc
//
LOCUS       DBMDSQ010000119         5348 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_3748_length_5348_cov_2.259005, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000119 DBMDSQ010000000
VERSION     DBMDSQ010000119.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 5348)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5348)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5348
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3748_length_5348_cov_2.259005"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..792
                     /locus_tag="ACZPAX_05515"
     CDS             <1..792
                     /locus_tag="ACZPAX_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HIR1113391.1"
                     /translation="AFQNALADVFEVIDNANKYIDATAPWVLAKSEESKPRLARVLYN
                     LAETLRICTVLLQPFMPTTCEKIFAQLNVAADGKTWDSAASFGTLPANATLHKGENIF
                     PRIDAAKELAELEALEAAQKAAAQAANAPAEEPKKEEAAAASAELIGEHEEEISFDDF
                     CKVELRVAEVRACERMKESKKLLHLTVFDGERERCILSGIAKWFAPEDLLGKKIGIVA
                     NLAPRPMMKGKYVSEGMIFAADTDVDGGCAIAFFPDSTPAGARIH"
     gene            802..1740
                     /locus_tag="ACZPAX_05520"
     CDS             802..1740
                     /locus_tag="ACZPAX_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005922266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR1113392.1"
                     /translation="MQKSKLDQFFSIPAAAVLMAVVCNVLWGSAFPFIKLGYRLFAID
                     SADTASILCFAGVRFMMGAALVWLAGLALNRRPLPMPHGKTLASACGLGLWQTAAQYF
                     FYYSAVALLTGAMGGILNSTQSFMGVILAHFLYGKADRMTPRKALGCALGFCGVLIAT
                     LGNHGSGSAKGMAFMLIASAIFASAGPWNKAVTQHADSFSVCVLNLGVGGLALAVMGF
                     AMGGGLHPESAAGIPVLLFLAFISGAGYVIWALLMKNNPVSRIAVFGLIIPVMNVLLS
                     ALLNGEPLFEWNYLAALLLVCIGIYLVNRAPQEAKA"
     gene            1737..2528
                     /locus_tag="ACZPAX_05525"
     CDS             1737..2528
                     /locus_tag="ACZPAX_05525"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004536 - DNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HIR1113393.1"
                     /translation="MTNIFDTHAHYSSRQFDADRAALLTALPAGGVVGVLECATHSGD
                     AARVLELAHAAPYIHAAIGIHPESLIEEDAATVAVYHGDWRAELAAMRPLFEDKSVAA
                     VGEIGLDHHWPVPRQEQYDLFEAQLQLAKELDLPVSVHDREAHAEMYELLRKYKPRGA
                     LHCYSGSADDAAWLVEQGMYLGFGGAVTYKGAKRAAKVLAAIPHERALLETDCPYMAP
                     EPVRGTRNDSRNIAHVAAYIGTIWDMDAQAVLDQTAQNARACFGV"
     gene            2582..2815
                     /locus_tag="ACZPAX_05530"
     CDS             2582..2815
                     /locus_tag="ACZPAX_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015565926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate dehydrogenase"
                     /protein_id="HIR1113394.1"
                     /translation="MKARIPKHREFIIDFPQDMDQAKANEGWNKLNEIVEEYKKAHNG
                     QSVYSATFIEDCEPAVKKLQEEYGFNYTIQEIK"
     gene            2876..3349
                     /locus_tag="ACZPAX_05535"
     CDS             2876..3349
                     /locus_tag="ACZPAX_05535"
                     /inference="COORDINATES: protein motif:HMM:NF024698.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HIR1113395.1"
                     /translation="MQKTVIRPAGEGDIPAIIALYRELDAAMVDLQPEFFCAAPREEA
                     EISSAVKAQDADFLLAEQDGTPVGFALVTYAGWTPAYSCVLPHRYARLEDLIVGKAYR
                     GRGIGGELLAAAKRWARNRRLEYVELQVLAQNEGAQHLYESQDFVEATKVMRCML"
     assembly_gap    4504..4513
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4611..>5348)
                     /locus_tag="ACZPAX_05540"
     CDS             complement(4611..>5348)
                     /locus_tag="ACZPAX_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005931205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DALR anticodon-binding domain-containing
                     protein"
                     /protein_id="HIR1113396.1"
                     /translation="PTYFAADIAYHYNKLAVRGFDKAIDVWGADHHGHVARMKGAMDA
                     IGLDGSRLDIVLMQMVNLMRDGKPVRMSKRTGKAITLTDLLDEVPIDSARFFFNQRES
                     SSTLDFDLDLAVRNDSENPVYYVQYAHARICSVLKKLESEGVKFAGADAVDASVLTDP
                     AEQALIRLLAAFPAEIAAAAEKYDPARITRYCIDVASAYHRFYNACRILDAEGTVQQG
                     RIALCLAVRGVIHNILTMFKVNAPETM"
BASE COUNT          978 a         1534 c         1657 g         1169 t
ORIGIN      
        1 gccttccaga atgcgctggc tgatgtgttc gaggtcatcg acaacgcaaa caagtatatc
       61 gacgccaccg cgccgtgggt gctggctaag agcgaggaat ccaagccccg cctggcccgc
      121 gtgctgtaca acctggccga gacgctgcgc atctgcaccg tgctgctgca gccgtttatg
      181 cccaccacct gcgagaagat ttttgcccag ctgaacgtgg ccgccgacgg caagacctgg
      241 gacagcgccg ccagcttcgg cacgctgccc gccaacgcca ccctgcacaa gggcgagaac
      301 atcttccccc gcattgacgc cgcaaaggag ctggccgagc tggaagccct ggaggctgcc
      361 cagaaggccg ccgcccaggc tgccaacgca cctgccgagg agccgaagaa agaggaggct
      421 gccgctgctt ctgcggagct tatcggtgag catgaggaag aaatcagctt cgacgatttc
      481 tgcaaggtcg aactgcgcgt ggccgaggtc cgcgcctgcg agcgcatgaa ggagagcaag
      541 aagctcctgc acctgaccgt gttcgacggc gagcgcgagc gctgcatcct gtccggcatt
      601 gcgaagtggt ttgcacccga ggatctgctc ggcaagaaga tcggcattgt cgctaacctg
      661 gccccccgcc cgatgatgaa gggcaagtat gtcagcgagg gtatgatctt tgccgcggac
      721 accgatgtgg acggcggctg cgccatcgcg ttcttcccgg acagcacccc cgcgggcgca
      781 cgcatccact aaggcggcgc tatgcaaaaa tctaagttag accaattttt ctccatcccg
      841 gcggcggctg ttttgatggc cgttgtctgc aatgtgctgt gggggtcggc gttccccttc
      901 atcaagctgg gttaccgcct gtttgcgatt gattctgccg atacggcgtc gattttgtgc
      961 ttcgcgggtg tccgttttat gatgggcgcg gcgctggtct ggctggcagg tctggcgctg
     1021 aaccgccgcc cgctgccgat gccgcacggc aaaacgctgg cttcggcctg cgggctgggc
     1081 ctgtggcaga ccgctgcaca gtatttcttc tactactccg ccgtggcgct gctgaccggc
     1141 gcgatgggtg gtatcctgaa cagcacccag agctttatgg gtgtgatcct ggcgcacttt
     1201 ttgtacggca aggccgaccg gatgaccccg cgcaaggcgc tgggctgtgc gcttggcttt
     1261 tgcggtgttc tgattgccac gctgggcaac cacggcagcg gcagcgcaaa ggggatggcg
     1321 ttcatgctga ttgcctccgc aatctttgca tcggccgggc cgtggaacaa ggccgtgacc
     1381 cagcatgcag acagcttttc ggtctgtgtg ctgaacctcg gtgttggtgg tctggcgctg
     1441 gctgtgatgg gctttgccat gggcggcggt ctccaccccg agagcgccgc aggtatcccg
     1501 gtgctgctgt ttttggcgtt tatctccggt gcggggtatg tgatctgggc gctgctgatg
     1561 aaaaacaacc cggtctcccg cattgcagtg ttcggtctga tcattccggt catgaatgtg
     1621 ctgctgtcgg cgctgctgaa tggcgagccg ctgtttgagt ggaactatct cgccgcgctg
     1681 ctgctggtct gcatcggcat ttatctcgtc aaccgcgccc cgcaggaggc caaggcatga
     1741 cgaacatttt tgacacccac gcgcactatt cctcgcgtca gtttgacgct gaccgcgccg
     1801 cactgttgac tgctttgcct gcgggcggcg tggtgggcgt gctggaatgt gccacccact
     1861 ctggtgatgc cgcccgcgtg ctggagctgg cgcatgccgc gccctacatc catgcggcga
     1921 tcggcatcca cccggaaagc ctgatcgagg aagacgctgc caccgtggcc gtttatcacg
     1981 gcgattggcg tgccgagctg gccgccatgc gcccgctgtt tgaagataag tcggtcgcag
     2041 cggtggggga aatcggcctc gaccaccact ggccggtgcc ccggcaggag cagtacgacc
     2101 tgtttgaggc acaattacag cttgcgaaag agctggattt gccggtgtcg gtccacgacc
     2161 gcgaggccca cgccgaaatg tacgagctgc tgcgcaaata taagccgcgc ggcgcactgc
     2221 actgttacag cggcagcgcc gacgacgcgg cctggctggt ggaacagggt atgtacctgg
     2281 gttttggcgg cgctgtgacc tacaaagggg ccaagcgcgc ggccaaggtg ctggccgcca
     2341 tcccgcacga gcgggcgctg ctggaaaccg actgccccta catggccccc gagccggtgc
     2401 gcggcacccg caacgacagc cgcaacattg cccacgtggc ggcgtacatc ggtacgatct
     2461 gggacatgga cgcccaggct gtgctggacc agaccgccca aaacgccagg gcttgctttg
     2521 gcgtgtaaaa tgctgtaata tacagatgta tattgaataa aaaattcagg agtgacttac
     2581 catgaaagca cgtatcccta aacatcgcga atttatcatt gatttcccgc aggatatgga
     2641 tcaggccaag gctaacgagg gctggaacaa gctgaacgag atcgtggagg agtacaagaa
     2701 ggcccacaac ggccagagcg tttactccgc gacctttatt gaggactgcg agcctgctgt
     2761 gaagaagctg caggaagagt acggcttcaa ctacaccatt caggaaatca agtaatttgc
     2821 accggcggac aactgcgtcc gccggttttt ggttatggca aagggggaga caagaatgca
     2881 aaagaccgtg atacgcccgg cgggggaggg ggatatcccc gccatcattg cgctgtaccg
     2941 cgagctggac gccgccatgg tggatctgca gccggagttt ttctgcgcgg ccccgcggga
     3001 agaagcggag atcagcagcg ccgtcaaggc gcaggacgcg gattttctgc tggctgagca
     3061 ggacggcaca ccggtgggct ttgcgcttgt cacctacgcc gggtggacgc ccgcgtacag
     3121 ctgtgtgctg ccccaccgct acgcccggct ggaagatctg atcgtcggca aggcttaccg
     3181 cggccggggc atcggcggcg aactgctggc cgccgccaag cgctgggccc ggaaccggcg
     3241 gctggaatac gtagagctgc aggtgctggc ccagaacgag ggcgcgcagc acctgtatga
     3301 gtcgcaggat tttgtcgagg caacgaaagt tatgcggtgt atgctgtgaa aaccgctctg
     3361 taggggccgg gcatgcccgg cccgcaggtt tacggttgca cccctttcgc agttgatttg
     3421 cagggcgggg cttgacccct ttgcggggcg tcggggacgc cgccccctac aggcataacc
     3481 cgcaaagttt ctcactcccg caaatgggca cggcggccag agggctggcc gccctacgct
     3541 ctcccgtgaa ttaggtttgc agggggggat tcatccctcc cgggttgtgc gcccatattg
     3601 ctgcacaaca caaaaagccg gggcgaacat ttcgttcgcc ccggcggtct tacttcttcg
     3661 gagtcatagc ctcataaccc ttttcgttat ttttcggact gtttccgcat cgtcatggga
     3721 atcatcctat ctttttgcga attttgatgg ccctttcagg atcttactaa ctgttcattg
     3781 gacttaccct ctggttcgtt atttgctcaa aagcatttgt ggaacggagt aatggtcgtt
     3841 attaagatcg ttcctggggt ctgggtctga tgctgaggac caaagcctct taactctagt
     3901 acattggagt ttctccttgg taatcaggtg aaattcagct gcttcctgtt cggcggagtg
     3961 gtgacggtca tctctaccag ccttctttct tcctgatcta tatgttatgc tgggggtctg
     4021 ttttgacggc ccctgggtct gctaaacgaa gttgtaagca accgtgcatc gcgttctttt
     4081 tgctttctgt acaggttatg acctgcggct ttggatgcgg tccttatctt gtcagcaggc
     4141 ccaggcccgc cggtggtgaa tgggatcttt gaagtttgcc attggacttg agcctccttc
     4201 cttacgaatc ctagccggcg gcctttactt tgtctttccg tcttctgccc tcgtgctgct
     4261 ggatcggagt ccttaaagtg cttccttttt cactccccga ctttagggaa cttctggaag
     4321 atcttcttta agatgtcggt ctgttcacga tcggtcttta tgaagatttg ttggaaagtg
     4381 gcaactcttg gagttcttaa ctccttatct ccggctgttt tctttctgtg actatactat
     4441 atcacagcct tgggatttcg tctattcgca gaccgcacaa gctttatatg caatatttgt
     4501 gcannnnnnn nnngcgcgtg tacgccaaag gaaaagccgc agcttcgtcg gaaagctgcg
     4561 gctcttattt tatgaagcct tccccccaag ggggaaggct tggagctttt ttacatcgtc
     4621 tccggggcgt tcaccttgaa catcgtcaaa atgttatgga tgaccccgcg cacggccagg
     4681 cagagggcaa tgcggccctg ctgcacggta ccctcggcat ccaggatgcg gcaggcgttg
     4741 tagaagcggt ggtaggcgga cgccacatca atgcagtagc gggtgatgcg ggccgggtca
     4801 tacttctcgg cggcagcggc gatctcagcg gggaaggcgg ccagcaggcg gatgagcgcc
     4861 tgctcggcgg ggtcggtcag cacgctggcg tccacggcgt ccgcgcccgc aaacttcacg
     4921 ccctcgctct cgagcttttt cagcaccgag cagatgcgcg cgtgggcgta ctggacatag
     4981 tagaccgggt tctcgctgtc gttgcgcacg gccaagtcga ggtcaaagtc cagcgtggag
     5041 gagctctcgc gctggttgaa gaagaaccgc gcggaatcga tgggcacttc gtccagcagg
     5101 tcggtcaggg tgatggcctt gccggtgcgc ttgctcatac gcacgggctt gccgtcgcgc
     5161 atcaggttga ccatctgcat cagcacgatg tccaggcggc tgccgtccag accgatggcg
     5221 tccatcgcac ccttcatgcg ggcgacatgg ccgtggtggt cggcgcccca gacgtcgatg
     5281 gccttgtcaa agccgcgcac ggccagcttg ttgtagtggt aggcgatatc ggccgcaaaa
     5341 taggtggg
//
LOCUS       DBMDSQ010000120         5308 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_3780_length_5308_cov_1.782792, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000120 DBMDSQ010000000
VERSION     DBMDSQ010000120.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 5308)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5308)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5308
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3780_length_5308_cov_1.782792"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            12..85
                     /locus_tag="ACZPAX_05545"
     tRNA            12..85
                     /locus_tag="ACZPAX_05545"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:46..48,aa:Thr,seq:tgt)
     gene            314..745
                     /locus_tag="ACZPAX_05550"
     CDS             314..745
                     /locus_tag="ACZPAX_05550"
                     /inference="COORDINATES: protein motif:HMM:NF024854.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIR1113397.1"
                     /translation="MVPILSYAQHYKKYYTVQFEGAAAAYGLNQLEIDILLFLHNNPE
                     LCTASDICRYRALAKSNVSAAVERLRSRGVLTVSPAPDNRRQRLLGFTDEGCTMAAAL
                     AKIQRRSIEPLFAGFTLEEQQALQDYLRRMDANILRCLQKE"
     gene            750..1523
                     /locus_tag="ACZPAX_05555"
     CDS             750..1523
                     /locus_tag="ACZPAX_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015566287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HIR1113398.1"
                     /translation="MTYLIKFIVCFAAGIGAGLGTGFAGMSAAAVISPMLITFLGMDP
                     YMAVGIALASDVLASAISAYTYGKNGNLDIKNGAVMMAAVLCFTLVGSYVASLLPGTT
                     MGGFSTFMTFLLGVKFIVRPVMTTKSAMSAVSAQKRFMQSLLCGAGVGFICGFVGAGG
                     GMMMLLLLTSVLGYELKTAVGTSVFIMAFTAFTGSVSHFAIGGAPDLFCLVCCVLSTL
                     LWARIAAKFANKAEPATLNRATGVVLVILGAVIMAVNYL"
     gene            complement(1595..2869)
                     /locus_tag="ACZPAX_05560"
     CDS             complement(1595..2869)
                     /locus_tag="ACZPAX_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007048601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR1113399.1"
                     /translation="MTTLPDIPRLYTALAEVLAAVLYAQAMPPRMDKRLCWGITVVWA
                     GLLAVFLQLTGDVPLVWWIPCMTAAIGWIYLYLWGTRAMNLLEAGYSCARAFILAELA
                     ASVEWQLHCVLWPQQGAGKPLSLVLLAVVYAALYGFLYLFEQRRATADQLTITTAAML
                     MAVVMAVTVFAVSNLSFVSNGEATMSVFYIRTLVDFAGVLILSVQHEQLRENALHSEL
                     TAMDNVLRRQYEQYKQSKENIRLINRRYHELKMQIAAIRAERDRAKQDAALAEMESGI
                     RKYEAENKTGNPVLDTLLTAKSLYCQQHGINMTCVADGKLLDFLSTGEICTIVGTALD
                     NATESVSAEPDPEKRLIRVAIYAQNGFVMLRFENYCASPVELAADGLPKRSAHGGYDL
                     KSVRAAAENHGGTLTLHWENEWFTLRVLLLQK"
     gene            complement(2866..3576)
                     /locus_tag="ACZPAX_05565"
     CDS             complement(2866..3576)
                     /locus_tag="ACZPAX_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015564571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HIR1113400.1"
                     /translation="MIRVAIVEDEAAVRDQLMGYVQRYMRQYNAQIEVTMFTDGVEIL
                     EGYRPVYDIIFLDVEMQHLDGMETASRIRALDSDVLLIFITNMAQYAIKGYAVGALDY
                     VLKPVPYFAFSQQLQKAAGQLARRARRYLAVPVDGGMRRLDEASVYYIESEGHRIHFY
                     TEDGDFSAPGALKAWEEKLEPHGFARCNSGYLVNLAYVSSVQMNTVQVGPHQLQVSRP
                     RRKAFLEALADYIGENGA"
     gene            complement(3576..4685)
                     /gene="glf"
                     /locus_tag="ACZPAX_05570"
     CDS             complement(3576..4685)
                     /gene="glf"
                     /locus_tag="ACZPAX_05570"
                     /EC_number="5.4.99.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009984514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008767 - UDP-galactopyranose mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009273 - peptidoglycan-based cell wall
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
                     /protein_id="HIR1113401.1"
                     /translation="MYDYLVVGSGLYGAIFAHEAKAHGKSVLVVDKRPNIAGNIYTEN
                     IEGINVHKYGAHIFHTNNKKVWNYITKFAEFNRFTNSPVANYKGELYSLPFNMYTFNK
                     MWGVVTPEEAAAKIEEQRKEITHEPQNLEEQAISLVGRDIYEKLIKGYTEKQWGRDCK
                     DLPAFIIKRLPVRLTFDNNYFNALYQGIPIGGYTKMIANLLDGIEVRLNTDYLENKAE
                     LDALADKIVYTGPIDAYFGYKLGTLEYRSVRFENELLDKPSFQGNAAVNYTDRETPWT
                     RIIEHKWFEFGKDENGNDLPKTIISREYSSEWKPGDEPYYPVNDAKNGALYAEYKKLA
                     DAEPKVIFGGRLGEYKYYDMDQVIAAVLDRCETEL"
     gene            complement(4699..>5308)
                     /locus_tag="ACZPAX_05575"
     CDS             complement(4699..>5308)
                     /locus_tag="ACZPAX_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002847192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP binding domain-containing protein"
                     /protein_id="HIR1113402.1"
                     /translation="ALRVAYFNELDTYAESKGLDTRQIIDGVCLDPRIGSHYNNPSFG
                     YGGYCLPKDTKQLLANYADVPENLIEAIVESNRTRKDFIADRVLRLAGYYGYGEDNEY
                     DEAQEKPVTIGVYRLTMKSNSDNFRQSSIQGVMKRIKAKGATVIIYEPTLKDGETFFG
                     SRVVNDLDAFKAQSQAIIANRYDACLDDVKEKVYTRDLFRRD"
BASE COUNT         1018 a         1544 c         1532 g         1214 t
ORIGIN      
        1 cttacccacc cgcctcttta gctcagttgg ccagagcacg tgatttgtaa tctcggggtc
       61 gttggttcga atccgacaag aggtcaaggg ttcgaatccc tccgggtcca ccataaatcc
      121 tcaccgtgtt gtggtgggga tttttatttt taccataaga ttgcacaaat ttttaaaggc
      181 cgctttgcac aagatttaca aaaccggcag taggcaaaat gcctgctgcc ggttttgtgt
      241 attgactttt ggggaataaa tagttataat tagaacagtt ctaaatcgaa gcattgcaaa
      301 ggaggtcctg cgcttggtcc cgattttatc ctacgcgcag cactacaaaa agtactatac
      361 tgtgcagttt gagggcgcgg cggccgcgta tgggctgaat cagttagaaa tcgacatttt
      421 gctgttcctg cacaataacc cggagctttg cacggccagt gacatctgcc gctaccgtgc
      481 gctggcaaag tccaatgtgt ctgcggcggt ggagcggctg cgcagccgcg gggtgctgac
      541 cgtctcccct gcaccggaca accgccgcca gcggctgctg ggctttaccg acgaaggctg
      601 cacgatggct gcggcgctgg ccaagatcca acgccgcagc atcgagccgc tgtttgccgg
      661 cttcacgctc gaagaacagc aggccttgca ggactatctg cgccgcatgg atgccaacat
      721 cctgcgctgc ttacaaaagg agtaatcgta tgacatacct tatcaagttt atcgtctgct
      781 ttgcggcagg catcggcgcg gggctgggca ccggcttcgc gggcatgagt gctgcggcgg
      841 tcatctcccc catgctcatc acgtttttgg gcatggaccc ctacatggcc gtgggcatcg
      901 cgctggcaag cgacgtgctg gcaagcgcga tctcggccta cacctacggc aaaaacggaa
      961 acctggacat taaaaacggc gccgtcatga tggcggcggt actctgcttc acgctggtgg
     1021 gcagctatgt agccagcctg ctgcccggta cgacgatggg cgggttctcg accttcatga
     1081 ccttcttgct gggcgtcaag ttcattgtgc ggccggtcat gacgacgaaa agcgccatga
     1141 gcgctgtcag tgcccaaaag cggttcatgc agtcgctgct ctgcggcgcg ggcgtgggct
     1201 ttatctgcgg attcgtcggc gcgggcggcg gtatgatgat gctgctgctc ctgacgtcgg
     1261 tgctgggcta cgaactgaaa accgccgtcg gtacgagcgt ttttatcatg gcattcacag
     1321 cgttcaccgg ctcggtcagc cactttgcca tcggtggcgc gcccgatctg ttctgtcttg
     1381 tctgctgcgt tctcagcacg ctgctgtggg ctcgcatcgc cgcaaagttc gccaacaagg
     1441 ccgagcctgc caccctgaac cgcgccaccg gcgtggtgct ggtgattctg ggcgcagtga
     1501 ttatggcggt gaattatctg taagattgcc tttgtcccat aaagcacaaa aatccatctc
     1561 aagcttctcg cttgagatgg atttttcttt cctgttattt ttgtagcaac agtacccgca
     1621 gggtgaacca ctcgttctcc caatgcaggg tcaaggtgcc gccatggttt tcggcggcgg
     1681 cgcgcacgct ctttaaatcg tacccaccat gggcgctgcg tttgggcaga ccgtcggcgg
     1741 ccagctctac cgggctggcg cagtagtttt caaaccgcag catcacaaaa ccgttctgtg
     1801 cataaattgc cacgcggatg agccgttttt ccgggtcagg ctcggcggaa acgctctcgg
     1861 tggcgttatc cagcgccgtt ccgacgatcg tgcatatttc ccctgtcgag aggaaatcca
     1921 acagcttgcc gtcggctacg caggtcatat tgatgccgtg ttgctggcag tacaggcttt
     1981 tcgcggtcag cagcgtgtcc aaaaccgggt tgccggtctt gttctcggcc tcatacttgc
     2041 ggatgccgct ctccatctcg gccagggccg cgtcctgttt ggcccgatcg cgctcggcac
     2101 ggatggccgc aatctgcatt ttcagttcgt ggtagcgccg gttgataagg cggatgtttt
     2161 ctttgctctg tttgtactgc tcgtactggc ggcgcaggac attgtccatg gcggtcagct
     2221 cgctgtgcag ggcgttctcc cgcagctgct catgctggac gctcaaaatc agcacgcctg
     2281 caaagtccac cagcgtgcgg atgtagaaaa cgctcatcgt cgcctcgcca ttggagacaa
     2341 aactcagatt gctgactgca aagaccgtga ccgccatgac caccgccatc agcatggctg
     2401 ccgtggtgat ggtcagctgg tccgccgtag ctcggcgctg ttcaaacaga tacaaaaatc
     2461 catatagtgc cgcatagacc accgccagca gcaccagcga gagcggcttg cccgccccct
     2521 gctgcggcca caaaacgcag tgcagctgcc attccacgct ggcggccagc tcggccaaaa
     2581 tgaacgctcg ggcacagctg tacccggctt ccagcaggtt catggcccgc gtgccccaca
     2641 agtagagata aatccacccg atggccgccg tcatgcaggg tatccaccac accagcggca
     2701 cgtcgccggt cagctgcaaa aagacagcca gcagccccgc ccaaacgacg gtaatgcccc
     2761 agcagagacg tttgtccatg cgcggcggca tggcctgggc atacaaaacc gccgccagca
     2821 cctctgccaa ggccgtgtac aggcgcggga tatccggcag tgtcgtcatg cgccgttctc
     2881 tccgatgtaa tcggccagcg cttccaaaaa cgccttgcgc cgagggcgac tgacctgcag
     2941 ctggtgcggg ccgacctgca cggtgttcat ctgcacgctg gaaacatacg ccaaattaac
     3001 cagatagccg ctgttgcagc gggcaaagcc atggggttcc agcttttcct cccaggcttt
     3061 cagtgcgccg ggagcggaga aatcaccgtc ctcggtgtaa aaatggatgc ggtggccctc
     3121 gctctcgatg taatagaccg atgcttcatc cagccgccgc ataccaccgt ccaccggcac
     3181 ggccagatac cgccgcgccc gccgggccag ctggcctgcg gccttttgaa gctgctgtga
     3241 gaaggcaaaa tacggcaccg gctttaacac gtaatccagt gcgccgacgg cgtacccctt
     3301 gatggcgtac tgggccatgt tcgtaatgaa gatgagcagc acgtcactgt ccaatgcgcg
     3361 gatgcggctg gcggtctcca tgccgtcgag gtgctgcatc tccacatcca agaaaataat
     3421 gtcgtagaca ggccgatatc cttccagaat ttccacaccg tcggtgaaca tcgtcacctc
     3481 gatctgggcg ttgtactggc gcatatagcg ctgcacatag cccatcagct ggtcgcggac
     3541 agcggcctca tcctctacga tggcgacgcg gatcattaca gttcggtctc gcagcgatcc
     3601 agcacagcgg cgatgacctg atccatgtcg tagtatttgt actcacccaa acggccgccg
     3661 aagatgacct tcggctcagc gtcggccagc ttcttgtact cggcatagag tgcgccgttc
     3721 ttggcgtcgt tgaccggata gtacggctcg tcgccgggct tccactcact gctgtactcg
     3781 cggctgatga tggtcttggg caggtcgttt ccgttctcgt ccttgccgaa ctcgaaccac
     3841 ttgtgctcga tgatgcgggt ccacggtgtc tcgcggtcag tgtagttgac ggcggcattg
     3901 ccctggaagc tgggcttgtc cagcagctcg ttctcgaagc ggaccgagcg gtactccagc
     3961 gtgccgagct tatagccaaa gtaggcgtcg atagggccgg tgtagacgat tttgtccgcc
     4021 agtgcatcca gttccgcctt gttctcgagg taatcggtgt tcaggcgaac ctcaatgcca
     4081 tcgagcaggt tggcaatcat cttggtgtag ccgccgatgg gaatgccctg gtacagggca
     4141 ttgaaatagt tgttgtcgaa agtcaggcgc accggcaggc gcttgatgat gaaggccggc
     4201 aggtccttac agtcgcggcc ccattgcttc tcggtgtagc ccttgatgag cttttcgtag
     4261 atatcgcggc ccaccaggga gattgcctgc tcctcgaggt tctgcggctc gtgggtgatc
     4321 tccttgcgct gctcctcgat ttttgcagcg gcctcctccg gggtcacaac gccccacatc
     4381 ttattgaagg tgtacatatt gaagggcagc gagtacagct cacccttgta gttggccacc
     4441 ggcgagttcg tgaagcggtt gaactcagcg aattttgtga tgtagttcca tacttttttg
     4501 ttgttggtat ggaaaatatg ggcaccgtac ttgtggacgt tgataccctc gatgttttcg
     4561 gtgtagatgt tgcccgcaat gttcgggcgc ttgtccacga cgagaacgct ctttccatgg
     4621 gctttggctt cgtgggcaaa aatggcaccg tacaggccgg agccgactac gagataatca
     4681 tacataactt tttctccctt aatcgcggcg gaaaaggtcg cgggtgtaaa ctttttcttt
     4741 tacatcgtcc aggcaggcgt catagcggtt ggcaatgata gcctgggact gcgccttgaa
     4801 ggcatccagg tcgttgacga cccgcgagcc gaagaaggtc tcaccgtctt tcagcgtcgg
     4861 ctcatagatg atgaccgtcg cgcccttggc cttgatgcgc ttcatgacgc cctgaatgga
     4921 actctggcgg aagttgtcgg agttcgactt catcgtcaag cggtacacgc cgattgtgac
     4981 aggcttttcc tgcgcttcgt cgtactcgtt gtcctcgccg tagccgtagt aacccgccag
     5041 ccgcagcacg cggtcggcaa tgaaatcctt gcgggtgcgg ttggattcca cgatggcctc
     5101 aatcaggttt tccggcacat cggcgtagtt cgccaacagt tgcttggtgt ccttgggcag
     5161 gcagtagccg ccgtagccga agctgggatt gttgtagtgg ctgccgatac gcggatccag
     5221 gcagacgccg tcgatgatct gccgggtgtc caggcccttg ctctcggcgt aggtgtccag
     5281 ctcgttgaag taggccacac gcagcgcc
//
LOCUS       DBMDSQ010000121         5287 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_3798_length_5287_cov_1.917277, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000121 DBMDSQ010000000
VERSION     DBMDSQ010000121.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 5287)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5287)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5287
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3798_length_5287_cov_1.917277"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..2935)
                     /gene="cas9"
                     /locus_tag="ACZPAX_05580"
                     /gene_synonym="csn1"
     CDS             complement(<1..2935)
                     /gene="cas9"
                     /locus_tag="ACZPAX_05580"
                     /gene_synonym="csn1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009324328.1"
                     /note="Cas9, originally named Csn1, is the large,
                     multifunctional signature protein of type II CRISPR/Cas
                     systems. It is well known even to general audiences
                     because its RNA-guided endonuclease activity has made it a
                     popular tool for custom editing of eukaryotic genomes;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR RNA-guided endonuclease Cas9"
                     /protein_id="HIR1113403.1"
                     /translation="MKHPYGIGLDIGIASVGWAVVALNENAEPYGLIRCGSRIFDKAE
                     QPKTGESLAAPRREARSTRRRLRRRSLRKADLYELMAQNGLPGRAKIEEAVQAGHLPD
                     IYALRVQALDGPVTSMDFARILLHLMQRRGFRSNRKADDAQKDGKLLQAIDANTRRME
                     ENHYRTVGEMMYRDPVFAEHKRNKSENYLSTVRRDQIVEEAVQLFAAQRQYGAAWASP
                     EMEAEYLTILTRQRSFDEGPGGNSPYGGNIVEKMVGTCTLEGQAEPRAAKATWSFEYF
                     TLLQKINHIRIIESGAARILTAEERQELLSVCYQTDKLDFARIRKALALSEQARFNMV
                     RYRDGQTTEDCEKKEKIVCLPCYHKMRKVLNTLRKDYIRSVSRDRLDAAATALTMYKN
                     EATLRAKLEEAQFEPLEVDALMTLPSFSGFGHISVKACRKLIPYLEQGLNYNDACKEA
                     GYDFQGNYRGEKSQFLPASTEEMEDITSPVVRRAVAQTIKVVNAIIREQGESPVNIHL
                     ELAREMSKNFQQRNDLDKAMKDNNAENERLMKDLHELFPGRNITGQDLVKYRLWKEQD
                     GRCAYSLQPLELEKAITVSGYAEVDHIVPYSISFDDRRANKVLVLASENRQKGNRLPL
                     QYLQGKRRDDFIVYTKASVKNYRKRQNLLKEKLSEEDSKGFMQRNLQDTQYIASFMLN
                     YIRSHLAFADFPAAGKKRVVAVNGAVTAFLRKRWGLGKVRADGDLHHAVDAAVIACTT
                     DGMIQRVSRFYTHIETSDTRGERFPEPWPRFRDELMQRLSACPQENLMQINPVYYQNV
                     DIASIRPVFVSRMPRHKMTGAAHKETIKGRLDDAHTVQRRSITDLKLDKEGEIAGYFN
                     PSSDTLLYNALKEQLTAFGGDGKKAFAEPFFKPRADGTPGAQVRKVKVYDKATSMVAV
                     HEGKGVADNDTMVRIDVYFVPGDGYYWVPIYVADTVKPELPNKAVVAYKNYDEWKEMA
                     EENFFF"
     gene            complement(3161..4063)
                     /locus_tag="ACZPAX_05585"
     CDS             complement(3161..4063)
                     /locus_tag="ACZPAX_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007045967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HIR1113404.1"
                     /translation="MSKIGFLTDSCSDIPQELADKYGIEVVGFPINLDGVEYMERKDF
                     TNDQFYQMMRDAQGVPTTAAITQLQWCEIYERYVDEGYTDLVHLCINSAGSSTYNNAV
                     KGAEMLAEERPGHKLNIQLIDSHTYSMPYGWYLCECARKVRNGGELATCVEEMEQSLD
                     RVEICLAAYSLKQMKKSGRVSAAAAVVGDLLGIRPIISLNAGVSKVEAKVRGDAAVPA
                     AMIKWVESRVDSMKDTPYMVAYTSSTSKRDELVKACKKAFGHAPVIVFQLGGVVSANT
                     GPDGIAIVYPGHPRHLEAYAPQLP"
     gene            complement(4060..4569)
                     /locus_tag="ACZPAX_05590"
     CDS             complement(4060..4569)
                     /locus_tag="ACZPAX_05590"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007045968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0141098 - tRNA
                     (cytidine(34)-2'-O)-methyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HIR1113405.1"
                     /translation="MPTLNIVLVEPQIPQNSGNIARTCAVTGARLHMVGPMGFTIDDK
                     KLKRAGLDYWHQLDITYYKDLADFFARNKGPFFYFTSKGPRRHVDVHYPDGAYLVFGR
                     EDAGLPEELLAAHEADCVRMPMRRGERCLNLSNAVAVGAYEALRQWDFADLETRGQLT
                     RYEWSEDVK"
     gene            complement(4697..>5287)
                     /locus_tag="ACZPAX_05595"
     CDS             complement(4697..>5287)
                     /locus_tag="ACZPAX_05595"
                     /inference="COORDINATES: protein motif:HMM:NF020132.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HIR1113406.1"
                     /translation="YSGYLADSAQARLVEQAFELWPKALKVVDPVLGDGGRLYSGLGA
                     DMVPAMYNLCSKADLIVPNVTEAALLLGDPLPGVGSAEQAAAQAARLVRIAPQVVVTG
                     VTGLNGGRYIGCVGAARGGEGYTVKTPLLPRMFHGTGDIFAAVLVGRILQGNVPQAAV
                     QAAAAFVAECIRQTPEGADERLGVWLENALPKLRQE"
BASE COUNT         1050 a         1603 c         1361 g         1273 t
ORIGIN      
        1 agaaaaaaaa gttttcttcg gccatttcct tccactcatc gtaatttttg tatgcgacta
       61 cggctttatt gggcaattcc ggtttcaccg tatctgccac atagatcggc acccagtaat
      121 agccgtcgcc cggcacaaaa tagacatcaa tgcgtaccat cgtatcattg tcggccacac
      181 cttttccttc atggactgca accatgctgg tggctttatc atacacttta accttgcgca
      241 cctgtgctcc cggcgttcca tcggcacgcg gtttaaagaa cggttctgca aatgcctttt
      301 ttccatcgcc gccaaaagct gtcagctgtt cctttaaagc attgtacagc agggtatcac
      361 tgctcggatt aaaatatcct gcaatttctc cctctttgtc aagcttcaaa tctgtaatcg
      421 accggcgctg aaccgtatgc gcatcatcca agcggccctt tatcgtctcc ttatgtgccg
      481 ctcccgtcat cttatgccgc ggcatacgcg acacaaacac cggccggatg cttgctatat
      541 ccacattctg ataatatacc gggttgatct gcattaaatt ttcctgcggg caggccgaca
      601 aacgctgcat cagctcatcc cggaatcgcg gccacggctc cgggaagcgc tcaccccggg
      661 tgtccgaggt ttcgatatgc gtgtagaacc gcgacacccg ttggatcatt ccatccgtcg
      721 tgcaggcaat gaccgccgca tctacggcgt ggtgcaggtc gccgtcagca cgcaccttgc
      781 caagccccca gcgtttgcgc aaaaacgctg taactgcgcc gtttacagca accacacgct
      841 tcttacctgc cgcagggaaa tctgcaaacg caaggtggct gcgaatgtag ttcagcataa
      901 aacttgcaat atactgtgtg tcctgcaaat tacgctgcat aaaacctttg ctgtcttctt
      961 cgctcaactt ctcctttagc agattttgac gcttgcggta attttttaca cttgcttttg
     1021 tgtagacaat aaaatcatcc cgtcgttttc cttgcagata ttgcagcgga aggcgattgc
     1081 ctttctggcg gttttccgac gccagcacca atactttgtt cgcacggcgg tcgtcaaagc
     1141 tgatgctgta cggaacaata tggtcaactt ccgcataacc tgaaacagta atggcctttt
     1201 ccagttccag cggctgcagc gaatacgcac accgcccatc ctgttctttc cacaacctgt
     1261 attttacaag atcctggcct gtgatatttc tccctgggaa cagttcatgc aggtctttca
     1321 tcaaacgctc gttctcggcg ttattgtctt tcatggcctt gtcaaggtca ttgcgctgct
     1381 ggaagttttt gctcatctcc cgcgccagct ccaagtggat attgaccggg ctttctccct
     1441 gctcgcggat gatcgcattg acaactttga tagtctgcgc caccgcacgc cgcaccaccg
     1501 ggcttgtgat gtcctccatt tcctctgtgg acgcaggcag gaactgcgat ttttcaccac
     1561 ggtaatttcc ctgaaagtca tatcccgcct ctttgcaggc gtcgttatag ttcagtcctt
     1621 gttccaaata ggggatcagt tttcggcacg ctttgacaga aatatgcccg aacccgctaa
     1681 agctgggcag cgtcatcaaa gcatcaactt ccagcggttc aaactgcgct tcttccagct
     1741 ttgctctgag cgtagcttca tttttataca ttgtcagcgc cgttgccgct gcatccagcc
     1801 tgtcgcggct gacactgcgg atgtagtctt tccgcaaggt attgagcact ttgcgcattt
     1861 tgtggtaaca ggggagacat acaattttct cttttttctc gcaatcctca gttgtttgtc
     1921 cgtcccgata tcgcaccata ttgaatcttg cctgttccga cagggcaaga gccttgcgta
     1981 tcctggcaaa atccaacttg tccgtttgat agcatacgga aagtaattct tgccgttctt
     2041 ctgccgtcag tatacgtgcc gcaccgctct ctataatgcg gatatgattg attttttgca
     2101 gcagcgtaaa atattcaaac gaccaagttg ctttggctgc acgcggttcg gcttgtcctt
     2161 caagcgtgca ggttcccacc atcttttcaa caatgttgcc gccgtagggg ctgtttccgc
     2221 cggggccttc atcaaacgaa cgctgacgcg tcaaaatcgt aaggtactcc gcttccattt
     2281 ccggcgaagc ccacgccgca ccatactggc gttgcgcagc aaaaagctgt actgcttcct
     2341 ccacgatttg atcccgtctg accgtagaaa gatagttttc cgacttgttg cgtttatgct
     2401 ctgcaaacac cgggtcacgg tacatcatct cgccaaccgt gcggtagtgg ttttcttcca
     2461 tgcggcgcgt gttggcatcg attgcttgca gcagctttcc gtctttctgg gcatcatctg
     2521 ccttgcggtt gctgcggaaa ccacggcgct gcatcaagtg cagcaaaatt ctggcaaagt
     2581 ccatcgacgt tacagggcca tccaacgcct gcacacgcag ggcatagata tccggcaggt
     2641 ggcccgcctg caccgcttct tctattttcg ctctgcccgg caagccgttt tgcgccatca
     2701 gctcatacaa gtctgccttg cgcaagctgc ggcggcgcag tctgcggcgc gtgcttctgg
     2761 cttcccgccg cggcgcagca aggctttcgc cggttttggg ctgctccgcc ttgtcaaata
     2821 tgcgcgagcc gcagcggatc agtccataag gctccgcgtt ttcgttcagc gctaccacgg
     2881 cccagccaac agacgcaatg ccaatatcca ggccaattcc ataggggtgc ttcatattca
     2941 ccacaccttt tcttttttac tattatcata ccgcataaag aatcacacgg caactgacat
     3001 tttccacgaa aatttgacaa aaaagatgac gccctgtcat aagacagggc gtcagagctt
     3061 tacggtccta tgaccttatc atagcaacca taatagttct tggtatggta taagaactgt
     3121 ttatagtata caacagccct tgaaaaaatg caagtctttt ttacggcagc tgcggggcat
     3181 aggcctccag gtggcgggga tggccggggt agacgatggc aatgccgtcg gggccggtgt
     3241 tggcgctgac aacgccgccc agctggaaca caatgaccgg cgcatgaccg aatgcctttt
     3301 tgcaggcctt gacaagctcg tcgcgcttgg aggtgctgga cgtgtaagcc accatgtagg
     3361 gggtgtcctt catggaatcg acgcggctct ccacccattt gatcatggcg gcgggcacag
     3421 cggcgtcgcc gcgcaccttg gcctcgacct tggagacgcc cgcgttcaga ctgatgatgg
     3481 ggcggatgcc cagcaggtca cccaccacgg cagcggcggc gctgacgcgg ccggacttct
     3541 tcatctgctt caggctgtat gcggccaggc agatctccac gcggtccagg ctctgctcca
     3601 tctcctccac gcaggtggca agctcgccgc cgttgcgcac cttgcgggcg cactcgcaca
     3661 gataccagcc gtagggcatc gagtaggtgt ggctgtcgat gagctggatg ttcagcttat
     3721 ggccggggcg ctcctcggcc agcatctccg cacctttgac ggcgttgttg taggtactgg
     3781 atcccgcgct gttgatgcac agatgcacca ggtcggtata gccctcgtcc acatagcgct
     3841 cgtaaatctc gcaccactgc agctgggtga tggcggcggt ggtgggcacg ccctgggcgt
     3901 cgcgcatcat ctggtaaaac tggtcattgg tgaaatcctt gcgctccatg tattccacgc
     3961 cgtccaggtt gatggggaag cccacgacct cgatgccgta cttgtccgca agctcctgcg
     4021 gaatgtcgga acaggaatcc gtcagaaaac cgatcttgct cattttacat cctcactcca
     4081 ttcgtagcgg gtcagctgcc cgcgagtctc taagtcggca aaatcccatt ggcgcagggc
     4141 ctcatacgcg cccacggcca cagcgttgga caggtttagg cagcgctcgc cccggcgcat
     4201 gggcatacgc acgcagtcgg cctcgtgggc cgccagcagt tcctcaggca ggccggcgtc
     4261 ctcgcgccca aagacgaggt atgcgccgtc gggatagtgt acatccacat ggcggcgcgg
     4321 gcctttggat gtgaagtaga aaaacggccc cttgtttcgg gcgaaaaagt ccgccaggtc
     4381 tttataatat gttatatcaa gctggtgcca gtagtcaagc ccggcgcgct tcaatttttt
     4441 atcgtcgatg gtaaagccca ttggccccac catatgcagc cgcgcacccg tcaccgcgca
     4501 ggtccgggcg atattgccgg agttctgcgg tatctgcggc tcgaccaata caatgtttaa
     4561 ggttggcata tgtttacagt cctgtttgtt tgaattttgt gtggtggcag ctttgtaggg
     4621 cggccagtcc tctggccgcc acatcgccgc cctgttgcgg ggggcggcgt ctccgacgcc
     4681 ccgcctgtct gccgcttcac tcctgccgca gcttcggcag cgcattttcc agccagacac
     4741 ccaggcgctc atccgcacct tcgggggtct ggcggatgca ctcggccaca aaggcggctg
     4801 ctgcctgcac ggcggcctgg ggcacgttgc cctgcaaaat gcgccctacc agcacagcag
     4861 caaagatatc gccggtgcca tggaacatcc ggggcagcag cggcgttttg acggtgtagc
     4921 cctcgccgcc gcgggccgcg cccacacagc ctatgtaccg cccgccgttc agcccggtca
     4981 caccagtcac aacgacctgc ggcgcaatgc gcaccagccg tgccgcctgg gcggcggcct
     5041 gctcagcact gcccacgccg ggcagcgggt cgcccagcag cagggcagcc tcggtcacat
     5101 tgggcacgat caaatcagcc ttactgcaca gattgtacat agccgggacc atgtcggccc
     5161 ccagcccgct gtacagccgc ccgccgtcgc ccagcaccgg atccacgacc ttgagcgctt
     5221 tcggccacag ctcgaacgcc tgttccacca gccgggcctg ggcggagtcc gccagatacc
     5281 cggagta
//
LOCUS       DBMDSQ010000122         5284 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_3810_length_5284_cov_1.924561, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000122 DBMDSQ010000000
VERSION     DBMDSQ010000122.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 5284)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5284)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5284
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3810_length_5284_cov_1.924561"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..876)
                     /locus_tag="ACZPAX_05600"
     CDS             complement(<1..876)
                     /locus_tag="ACZPAX_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015537373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HIR1113407.1"
                     /translation="MARKNIRNFCIIAHIDHGKSTLSDRMLELTDSVDKRTMTDQVLD
                     DMDIEKERGITIKARAVTMRYKADDGETYEFNLIDTPGHVDFQYEVSRSLAACEGAVL
                     VVDASQGVEAQTLANCYLALDHNLEVVPILNKIDLPSADPDAVAKEVEDVIGLPCMEA
                     PRVSAKLGIGVKDVLECVVRDVPAPTGDPTLPLKALIFDSIYDSYKGVIVYVRIFEGT
                     VKPGDTIRLMSTGAEFQLVEVGHMGATQMTPCDKLEAGEVGYLTASIKTVRDTRVGDT
                     VTLAANPTAEPMPGFR"
     gene            complement(876..2057)
                     /gene="alr"
                     /locus_tag="ACZPAX_05605"
     CDS             complement(876..2057)
                     /gene="alr"
                     /locus_tag="ACZPAX_05605"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HIR1113408.1"
                     /translation="MSRLALEKHCWAEIDLTALRSNFEYIHRAVGGDICAVVKADAYG
                     HGDTTIAHVLQQAGAAAFAVSSLGEGRHLRRSGITKPILILGFADPSYAAVLAENDIA
                     TACFSTEYAQALSAAAVKAGVQAKVHLKIDTGMGRIGFAVRSGFDETIHELEALYTLP
                     GLDICGVFQHFAVADSNTPADTAYTDEQYALFARVVARLQADGFATGTVHCANSAAQL
                     RHPEWRHDMTRAGIILYGLDPSDDVRFPALQPVMSLKCVVTFVKDLQPGQSVSYGRTF
                     TAERPMRVATVCVGYADGYPRRLSGGLGVMNIHGHAAPVVGRVCMDQTMLDVTDIPDV
                     KMGDEVTVFGPGGGDTADTIAAKTQTINYEIVCGLARRVPRVYKENGKICEIWNDLEE
                     T"
     gene            complement(2113..3423)
                     /locus_tag="ACZPAX_05610"
     CDS             complement(2113..3423)
                     /locus_tag="ACZPAX_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HIR1113409.1"
                     /translation="MGKVRHCLRSAAGYLAVCAKWLVLAALVGCVVGPLGAAFGLALN
                     WANATRAAQPWLLYLLPIAGLVIVFLYSHFDPDGGGSTNQVFVSVREHKPMTLRTAPL
                     IFASTVMTHLFGGSSGREGAALLLGGSVSGQIGKVFHLENRDCRLMTMCGMAGAFSAI
                     FGTPLAATIFTLEVVDVGSMQYAALLPCLVSALLGVFISGRMGLAPESFVLKAEVAAT
                     PLNLVRVILLGALLAALSIFFCELLHTAPKLYKKFLPNAYLRVAAGGVLILALTKLLG
                     TTDYNGAGAAVIEAAINGEAVPYAFLLKMLFTALTLGAGFKGGEIVPIFFTGATFGCV
                     AAPLLGLPPQLGAALGMVALFCGCTNSPLASICLAIEVFGGQCIALFALACAVSYMLS
                     SYFSLYREQHFLHSKLRIVGVQRVHGRWSETDAKHFTTNDDGEN"
     gene            complement(3709..3784)
                     /locus_tag="ACZPAX_05615"
     tRNA            complement(3709..3784)
                     /locus_tag="ACZPAX_05615"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3749..3751),aa:Pro,seq:tgg)
     gene            complement(3949..>5284)
                     /locus_tag="ACZPAX_05620"
     CDS             complement(3949..>5284)
                     /locus_tag="ACZPAX_05620"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HIR1113410.1"
                     /translation="PNDTWYFEIRPATGWITPTQEAFDVLLLLVLPALAAAGYWQFEL
                     RRYKDAVHAAELEKAAREAQAANVAKTRFLFNMSHDIRTPMNAIIGYAGLLAAHLDER
                     DRALDYLGKIRSSSDFLLSLINYVLEMARIESGKAALKLEPSDINELAASLKAVFDPL
                     ADEKHITTTCTIDIQHPYVRSDQTKVREIFLNIISNAIKYTPEGGRVDFTMTELPGET
                     PGTVRYKAVVQDDGIGMSAEYLPHLFEEFSREHTSTESRVIGTGLGLPIVKSLVQLMG
                     GTIDVESTVGKGSTFTVVIPFPVISKEELIQQREQPADPAAGLAGKHLLLAEDNDLNA
                     EIALTILQENGMIVNRAEDGIRCLELLGAKPAGYYDAVLMDIQMPRMDGYQAAQAIRA
                     LPDARSTVPIIAMTANAFEEDKQRAFDAGMNDHISKPISVNALLAALRKNLK"
BASE COUNT         1069 a         1581 c         1618 g         1016 t
ORIGIN      
        1 gcggaaaccg ggcatcggct cggccgtcgg gttggcggcc agcgtcacgg tatcgccgac
       61 gcgggtgtcg cgcacagtct tgatgctggc agtcaggtag ccgacctcgc cggcctccaa
      121 tttgtcgcag ggggtcatct gggtcgcgcc catgtggccg acctccacca gctggaactc
      181 tgcgccggta ctcatcaggc ggatggtgtc accgggcttg acggtgccct caaagatgcg
      241 cacatagacg atgacgccct tatagctgtc gtagatggag tcgaagatca gcgctttcag
      301 cggcagcgtc gggtcgccgg tgggggcggg cacgtcccgc acgacgcact ccaaaacgtc
      361 cttaacgccg atgcccagct tggcggacac gcgcggggcc tccatgcagg gcaggccgat
      421 gacgtcctcg acctccttcg ccacggcgtc cgggtcggcg ctgggcaggt cgatcttatt
      481 caaaatcggt acgacttcca ggttgtggtc cagcgccagg tagcagtttg ccagcgtctg
      541 ggcctccacg ccctggctgg cgtccacgac cagcactgca ccctcgcagg cggccaggct
      601 gcggctgacc tcgtactgga agtcaacatg gcccggagtg tcgatgaggt tgaactcgta
      661 ggtctcgccg tcgtcggcct tatagcgcat cgtcacggcg cgggccttga tggtgatgcc
      721 gcgctctttt tcaatgtcca tgtcgtccag cacctggtcg gtcatcgtgc gcttgtcaac
      781 gctgtcggtc agctccagca tacggtcgga cagggtggat ttgccgtggt cgatgtgtgc
      841 gataatgcag aagttgcgga tgtttttgcg ggccatcagg tttcctccaa atcgttccag
      901 atctcgcaga tcttgccgtt ctctttgtat acgcggggca cgcggcgcgc cagcccgcag
      961 acaatttcgt agttgatggt ctgggtcttg gccgcaatgg tgtcggcggt gtcgccgccg
     1021 cccgggccaa agactgtgac ctcgtcgccc atcttgacat caggaatgtc cgttacatcg
     1081 agcatagtct gatccatgca gacgcggccc accacggggg cagcgtggcc gtgtatgttc
     1141 atgacgccaa gcccgccgga caaacggcgg gggtagccgt cggcgtagcc cacgcagacc
     1201 gtggcaacgc gcatcgggcg ctcggctgtg aaagtgcgcc catagctcac gctctggccg
     1261 ggctgcaagt ccttgacaaa ggtcacaacg catttcagcg acatgaccgg ctgcagggca
     1321 ggaaagcgga cgtcgtcgct ggggtcaagc ccgtacaaaa tgatgcccgc gcgggtcata
     1381 tcgtgccgcc actcggggtg gcgcagctgc gcggccgagt tggcacagtg tacggtgccg
     1441 gtggcaaagc catcggcctg caaacgcgct accaccctgg caaacagcgc atactgctcg
     1501 tcggtgtagg cggtgtccgc aggggtgttg ctgtccgcca cggcaaaatg ctggaacacg
     1561 ccgcagatat ccagccccgg cagggtgtac agggcttcca gctcgtggat agtttcatca
     1621 aacccggaac gcaccgcaaa gccaatgcgg cccatgccgg tgtcgatttt caggtgtacc
     1681 ttggcttgta cgcccgcctt gacggcggca gcggagagtg cctgcgcgta ctcggtagaa
     1741 aaacaggccg tggctatgtc attttctgcc aacactgccg cataggacgg gtcagcaaag
     1801 cccagaatca aaatcggttt tgtgatgccg ctgcggcgca ggtgccgtcc ctcgcccagg
     1861 ctgctgacgg caaacgccgc cgcaccggcc tgctgcaaga cgtgggcgat ggtcgtgtcg
     1921 ccgtggccgt aagcgtcggc cttgacaacg gcacagatat cgccgcctac cgcgcggtgg
     1981 atatattcaa aattgctgcg caatgctgtc agatcgatct ccgcccagca gtgcttttcc
     2041 agtgcaagtc tactcataaa cgcccattcc tccataacac ttaattatac catggaaaca
     2101 gcgttcccgc aactaatttt cgccgtcatc gtttgtggta aaatgcttgg catccgtctc
     2161 ggaccagcgg ccatggaccc gctgcacgcc cacaatgcgc agcttggagt gcagaaagtg
     2221 ctgctcacgg tagaggctga aatagctgga cagcatgtac gacaccgcac aggccagcgc
     2281 aaacagcgca atgcactgcc cgccgaatac ttcaatcgcc aggcagatgg acgccagcgg
     2341 gctgttggtg caaccgcaga aaagcgccac catacccagc gccgcgccca gctgcggcgg
     2401 cagacccagc agcggcgcag ctacacagcc gaacgtcgcc ccggtgaaga agatgggcac
     2461 gatctcgccg cccttgaacc ctgcacccaa ggtcagtgcg gtgaacagca tttttagcag
     2521 gaacgcatac ggcacggcct cgccgttgat ggcggcctcg atgaccgccg cgcctgcgcc
     2581 gttgtagtcg gtcgtgccga gcagctttgt cagtgccaga atcagcacgc cgcccgccgc
     2641 aacgcgcaga taggcgttgg gcagaaattt tttgtagagc ttgggcgcgg tgtggagcag
     2701 ctcacagaaa aatatgctca gcgccgccag cagagccccc aaaaggatga cccgcaccag
     2761 gttcagcggc gtggcggcga cctcggcttt caaaacgaag gattccggcg caaggcccat
     2821 ccgcccgctg ataaaaacgc ccagcagtgc cgaaaccagg caggggagaa gggccgcgta
     2881 ctgcatcgag ccgacatcca cgacttccag cgtgaagatg gtggccgcca gcggcgtgcc
     2941 gaagatggcc gagaacgcgc ccgccatgcc gcacatcgtc attaaacggc agtctcggtt
     3001 ttccaagtga aacaccttgc cgatctgccc ggacactgac ccgcccagca gcagggccgc
     3061 gccctcgcgc ccgctggaac cgccgaacag gtgggtcatg acagtggaag caaagatcag
     3121 cggcgcggtg cgcagcgtca tgggcttgtg ctcccgcacc gagacgaaca cctgattggt
     3181 ggaaccgccg ccgtcggggt cgaagtggct gtacaaaaac acgatgacca ggcccgcgat
     3241 gggcagcagg tacagcagcc acggctgggc cgcgcgggtg gcgttggccc agttcagcgc
     3301 cagtccgaac gctgcaccca gcggcccgac aacacacccc accagcgccg ccagcaccag
     3361 ccacttggcg cacaccgcca gataccccgc cgcgctgcgc agacagtgcc tgacctttcc
     3421 cataaagatt ccctcccgaa tattccgaaa tttgagatta ttatagcgtt tttgcgggga
     3481 aaagacaaca gaaaaatacc ctgccagggg caactcggca gggtatcgct tccatggtga
     3541 ggttatgcag caccacgtat acaggggcac aggctttgca gcgtggcagc gtagaagaac
     3601 atatacttga atagctggac agcaaaggcg cgtatctgta tgcagcgcgg taaaaaatgc
     3661 ggtataatac aaaaagacca cccggaaaca aacgtttctg ggtggttttg gtcgaggtga
     3721 caggactcga acctgcggcc tcgtggtccc aaaccacgcg cgctaccaac tgcgcaacac
     3781 ctcgttctta taacttactt attataatac acaagcggcg cgggcgtcaa gccctggctc
     3841 aacaaaaaag cggccctgtc gtattcatat ttcaggtact cggcaaaggt gatgggcttt
     3901 tggtcggcaa aaaatgtttt tcccggggga aaatttcctc tctgcaagtt atttcagatt
     3961 tttccgcagt gcggccaaca gggcgttcac gctgatgggt ttggagatat ggtcattcat
     4021 gccggcgtca aacgcgcgtt gcttatcttc ttcaaaggcg ttggccgtca tggcgatgat
     4081 gggcacggtg gaccgggcat caggcaaagc gcggatggcc tgcgctgcct ggtagccgtc
     4141 catgcggggc atctggatat ccatgagcac ggcgtcgtag tacccggcgg gttttgcacc
     4201 caaaagctcc aggcagcgga taccgtcctc agcgcggtta acgatcatgc cgttctcctg
     4261 caggatcgtc agcgcgatct cggcgtttag gtcgttgtcc tcggccagca gcaggtgctt
     4321 tcctgccagc ccagcggcgg ggtcggcggg ctgctcgcgc tgctggatca gctcttcttt
     4381 gctgatgaca gggaagggga taacaaccgt gaaggtcgaa cccttaccaa cagtgctctc
     4441 gacatcgatg gtaccgccca tgagctggac gagcgacttg acgatgggca ggcccaggcc
     4501 ggtgccgatg acgcggctct cggtgctggt gtgctcacga gaaaactcct caaacaggtg
     4561 ggggagatat tccgcactca taccgatgcc gtcatcctgc acgacagcct tgtaacgcac
     4621 ggtgccgggc gtttcgccgg gcagttcggt catggtgaaa tcgacgcggc cgccctcggg
     4681 tgtgtacttg atggcgttgc tgatgatatt taagaaaatt tcgcgtacct tcgtttggtc
     4741 gctgcgcaca tagggatgtt ggatatcgat ggtgcaggtg gtggtgatgt gcttttcgtc
     4801 ggccagtggg tcaaacacgg ctttcagcga ggcggcaagc tcgttgatgt cagacggctc
     4861 aagcttcaag gcagccttgc cgctctcaat gcgggccatt tccagcacat agttaatgag
     4921 ggagagcagg aaatcgctgg acgaacgtat ttttcccaga taatccagcg cacgatcccg
     4981 ctcgtccagg tgggctgcca gtagccctgc atagccaatg atggcgttca tcggggtgcg
     5041 gatgtcatgg ctcatattaa aaaggaaccg cgttttggca acgttggcgg cctgtgcctc
     5101 ccgtgcggcc ttttccagct cggcggcgtg gacggcgtcc ttgtagcggc gcagttcaaa
     5161 ctgccagtag ccggctgcgg ccagcgcggg cagcaccagc agcaaaagca catcaaaggc
     5221 ttcctgggtc ggggtgatcc agcccgtggc cgggcgtatc tcaaaatacc aggtatcgtt
     5281 gggc
//
LOCUS       DBMDSQ010000123         5259 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_3835_length_5259_cov_1.744087, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000123 DBMDSQ010000000
VERSION     DBMDSQ010000123.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 5259)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5259)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5259
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3835_length_5259_cov_1.744087"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1678
                     /locus_tag="ACZPAX_05625"
     CDS             <1..1678
                     /locus_tag="ACZPAX_05625"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008724621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HIR1113411.1"
                     /translation="VLDFIDTTREEGIPADGFQLSSGYCAVETPEGIKRCTFTWNHKR
                     FKNPADWFAQMEKRGIVVSPNIKPGMLLVHPLLDEMKQKGMFLPASDDNTGVDGLAVG
                     TWWGGPGLFVDYTKESTRLHWKQYIKDALLKYGCRSIWNDNCEYDSLVDKDAKVYFEG
                     KGSTIGTMKPVMSNLMCQLSNEAIEEYDPDCRPFSVCRSGHAGIQRYAQVWAGDNLTC
                     WDTLKYNIATILGMALCGVSNHGCDIGGFFGPAPEGELFVRWVQNGIFQPRFSIHSTN
                     TDNTVTEPWMYSDKKQYIKDAIDFRYKLSPTLYSLMERAHENGLPIWQPTFSVFQNDP
                     ATYDEGVDFMFGDSLLVANVVEKGQTVRPVYLPKTENEAERFYDFYTREAYAPGQTIE
                     VPVDIASIPLFVRSGAIIPMSGNALHNLMTEKTTALDILMAPDVDSAFTLYEDDGRTN
                     DYRKGAYLKTKIDVKAGVKTVVTFTNEGSYETAVESMALDMIHREKSPFYVQVDGKEL
                     PHFLHRRKFEEAESGWYYSQRLKSVQIKYPNPKKNHEVLVSFEQFDLIGM"
     gene            1819..2853
                     /locus_tag="ACZPAX_05630"
     CDS             1819..2853
                     /locus_tag="ACZPAX_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007045615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HIR1113412.1"
                     /translation="MFKKIAALGAAAALCVGLMSGCGAASTTADTSYSAENPLVLTLA
                     HGLSETHTVHIAMTQFADEVAEKTDGRIQVKIFPNGQLGSENENLEQLQAGVIAMTKV
                     SAPGLATYNEAFNAFGLPYIFNDTADFYNVMDSQEMQDFFLSTGDDGFVTLTYYTSGA
                     RSFYTKDKAIRTPADLKGLKIRVQDMKSQTDMMSYLGGIPVAMSYGDVYTSLQTGIID
                     GTENNETALTTGKHGEVCKVYSVDQHAMIPDVLIMSEKVWKTISPEDQQILLEAAHDS
                     TDAHKVMWDTAVEEAVKEAQETMGVEFVYDVDKEAFREATQPMIEAYEEEYPGVKTLM
                     DTIEKARGEE"
     gene            2857..3366
                     /locus_tag="ACZPAX_05635"
     CDS             2857..3366
                     /locus_tag="ACZPAX_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008724624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HIR1113413.1"
                     /translation="MKNTFAAIKYWMDKILSIACAILLSFMTILVLIQVFSRYVLNSP
                     VAFTEELVRYSLIWTGFIGAAYAFSTREHMSLTLVRDKFTGKAHTALLVAIDGLILLM
                     AIFVFTIGGFKLAVSASREFSALLGIPRSLVYAIAPISGVFIVIAQIINIYEDITGEK
                     VDSKEGADK"
     gene            3363..4664
                     /locus_tag="ACZPAX_05640"
     CDS             3363..4664
                     /locus_tag="ACZPAX_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006780811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HIR1113414.1"
                     /translation="MSITMTTVILFAVFGVLLFLGVPISISIVASSIVTAMSTLSWDQ
                     ITFITMQKMNSGVESFSLLAVPLFILAGNIMNNGGIAKRLVNFAQLFVGKIPGSMAQA
                     NILGNMLFGALSGSSVAAASAMGGCISPIEEENGYDPAYSAAANIASAPTGLLIPPTS
                     AFIVYSTVAGGVSISTLFMAGYIPGILMGLCCMIVAFIGAKKAGMKATGFDRSQSVLK
                     TVWDAVPSLLLIVIVIGGIVSGVFTATEGAGIAVLYCLILSIIYKSIDFKGFLNILLN
                     SAKTSGIILFLISASSAMSFVMAYSGIPAAISNGLMSLTDNKYIIFLLMNIILLVIGM
                     FMDITPAILIFTPIFLPIATSFGMTEIQFGVMLIFNMCLGNITPPVGSVLFVGCGIGH
                     VSIEQVTSKLIPYFVALVAALLAVTYIPALSLGLPSLMGLI"
     regulatory      4942..5155
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
BASE COUNT         1074 a         1666 c         1459 g         1060 t
ORIGIN      
        1 cgtgctcgac ttcatcgaca cgacccgcga ggagggcatc cccgcagacg gcttccagct
       61 gtccagcggc tactgcgcgg tcgagacgcc cgagggcatc aagcgttgca ccttcacctg
      121 gaaccacaag cggttcaaga acccggcaga ctggttcgcc cagatggaaa agcgcggcat
      181 tgtcgtctcg cccaacatca agcccggtat gctgctggtc cacccgctgc tggacgagat
      241 gaagcagaag gggatgttcc tgcccgcctc cgacgacaac accggtgtgg atggtctggc
      301 cgtcggcacc tggtggggcg gccccgggct gtttgttgac tacacgaagg aatccacccg
      361 tctgcactgg aagcagtaca tcaaggacgc gctgctcaag tacggctgcc gctccatctg
      421 gaacgacaac tgcgagtatg actccctcgt cgataaggac gcgaaggtct actttgaggg
      481 caagggcagc accatcggca cgatgaagcc ggtcatgagc aacctgatgt gccagctgtc
      541 caacgaggcc atcgaggaat acgaccccga ctgtcgcccc ttctcggtct gccgctccgg
      601 ccacgccggc atccagcggt atgcccaagt ctgggcgggc gacaacctga cctgctggga
      661 caccctgaaa tacaacattg ccacgatttt gggcatggcc ctctgcggcg tgtccaacca
      721 cggctgcgac atcggtggct tcttcggccc cgcgccggag ggtgagctgt tcgtgcgctg
      781 ggtgcagaac ggcattttcc agccgcgctt ctccatccat tcgaccaaca ccgacaacac
      841 cgtcaccgag ccgtggatgt acagcgacaa aaagcagtac atcaaggacg ccatcgactt
      901 ccgctacaag ctcagcccga cgctctactc gctgatggag cgcgctcacg agaacggcct
      961 gcccatctgg cagccgacgt tcagcgtgtt ccagaacgac ccggctacct acgacgaggg
     1021 tgtggacttt atgttcggcg actcgctgct ggtcgcaaac gtggtcgaga agggccagac
     1081 cgtccgcccg gtctacctgc ccaagaccga gaacgaagcc gagcggttct acgacttcta
     1141 cacccgcgag gcttacgccc ccggccagac gatagaagtg ccggtggaca tcgccagcat
     1201 cccgctgttt gtacgcagcg gcgccatcat cccgatgagc ggcaacgccc tgcataacct
     1261 gatgaccgag aaaaccacgg ccctggatat cctgatggcc ccggacgttg actctgcgtt
     1321 caccctgtac gaggatgacg gccgcacgaa cgactaccgt aagggtgcat acctcaagac
     1381 gaaaatcgac gtcaaggcag gcgtcaagac ggtcgtgacc ttcacgaacg agggtagcta
     1441 cgagaccgcc gtggagagca tggcgcttga catgatccac cgcgagaagt ccccgttcta
     1501 tgtgcaggtc gacggcaagg agctgccgca tttcctgcac cgccgcaagt ttgaggaagc
     1561 cgagagcggc tggtattaca gccagcgcct caagagcgtg cagattaagt accccaaccc
     1621 caagaaaaac cacgaggtgc ttgtctcgtt cgagcagttt gatttgattg ggatgtaagg
     1681 gggcggcggt gaggaatccc tccgccctcg cttcgctcgg ccacctccct ttgacaaggg
     1741 aggctgtccg cgaagcggat tgggggattc cccgctgcac ctgcctgcag cgaccacata
     1801 tatcagaagg agaaaattat gttcaagaaa attgcagccc tgggtgctgc ggcggcgctg
     1861 tgcgtcgggc ttatgtccgg ctgcggcgct gccagcacca cggccgatac cagctacagc
     1921 gccgagaacc cgctggtgct gacgctggcc cacggcctgt cggagaccca cactgtccat
     1981 atcgccatga cccagtttgc cgacgaagtc gccgaaaaga ccgatggccg catccaggtc
     2041 aagatcttcc ccaacggcca gcttggcagc gagaacgaaa acctcgagca gctgcaggcg
     2101 ggtgttatcg ccatgacgaa agtctcggcc cccggcctgg ccacctacaa cgaagcgttc
     2161 aatgccttcg gcctgcccta tatcttcaat gacaccgccg acttctacaa cgtcatggac
     2221 tcccaggaga tgcaggactt cttcctctcg accggcgacg acggctttgt cacgctgacc
     2281 tactacacct ccggcgcacg ttccttctac acgaaggaca aggccatccg caccccggca
     2341 gacctgaagg gcctgaagat ccgcgtgcag gatatgaaga gccagaccga tatgatgagc
     2401 tacctcggcg gcatcccggt cgccatgtcc tacggcgatg tctacacctc gctgcagacc
     2461 ggcatcatcg acggcaccga gaacaacgaa acggccctga ccaccggcaa gcacggcgag
     2521 gtctgcaagg tctattcggt cgatcagcac gccatgatcc ccgatgtact gatcatgagc
     2581 gaaaaggtct ggaagaccat cagcccggag gatcagcaga ttcttcttga ggccgcccac
     2641 gattccaccg acgcccacaa ggtcatgtgg gacaccgctg tggaggaggc cgtcaaggag
     2701 gcccaggaga ccatgggcgt cgaattcgtc tacgacgtcg acaaggaagc cttccgcgag
     2761 gcgacccagc ccatgatcga agcctacgaa gaagagtatc ccggcgtcaa gacgctgatg
     2821 gataccattg aaaaggcaag aggggaggag taagtcatga aaaacacctt cgcagccatc
     2881 aaatactgga tggacaaaat tctcagcatc gcctgtgcga ttctgctttc cttcatgacc
     2941 attctggtgc ttatccaggt gttctcccgc tatgtgctca acagcccggt cgcctttacc
     3001 gaggagcttg tccgttacag cctgatttgg accggcttca tcggcgcggc ctacgccttc
     3061 tccacccgcg agcatatgtc gctgaccctc gtccgcgaca agttcaccgg caaggcccac
     3121 accgccctgc tggtggccat cgacggcctg atcctcctga tggcgatctt cgttttcacg
     3181 atcggcggct tcaagctggc tgtttccgcc agccgcgagt tctcggcgct gctgggcatc
     3241 ccccgcagtc tggtttacgc tatcgccccg atttccggcg tgttcattgt gattgcgcag
     3301 atcatcaaca tctatgagga catcaccggc gaaaaagttg attccaagga gggtgcagac
     3361 aaatgagtat cactatgacc accgttattt tgttcgctgt gttcggcgtg ctgctgttcc
     3421 tcggcgtgcc gatctccatc agcattgtgg cgtcctccat cgtgaccgct atgtccaccc
     3481 tgtcctggga tcagatcacc tttatcacaa tgcagaagat gaactccggc gtggagagct
     3541 tctctctgct ggccgtaccg ctgtttattc tggccggcaa catcatgaac aacggcggta
     3601 tcgccaagcg cctggtcaac tttgcccagc tgttcgtcgg caaaattccc ggctctatgg
     3661 cgcaggccaa tattctgggc aatatgctgt ttggcgcgct gtccggctcc tccgtcgccg
     3721 ctgcgtccgc catgggcggc tgcatcagcc ctattgagga ggaaaacggc tacgaccctg
     3781 cctactctgc cgctgcgaac atcgcctctg cccccacggg cctgctgatt cccccgacct
     3841 ccgctttcat cgtctactcc accgtcgcgg gcggtgtctc catctccacc ctgttcatgg
     3901 ccggttatat ccccggcatt ctaatgggtc tgtgctgcat gatcgttgcg ttcatcggcg
     3961 caaaaaaggc cggcatgaag gccaccggct ttgaccgcag ccagagcgtg ctgaaaaccg
     4021 tttgggacgc ggttccgtcc ctgctgctca tcgtcatcgt catcggcggc atcgtctccg
     4081 gtgtctttac cgccaccgag ggcgcaggca tcgcggttct gtactgcctg atcctctcca
     4141 tcatctataa gagcatcgac ttcaagggct tcctgaatat cctgctcaac tctgccaaga
     4201 cctccggcat catcctgttc ctgatctccg cttcctctgc catgtccttc gtcatggcgt
     4261 actccggcat cccggcagct atctccaacg gtttgatgag cctgaccgac aacaagtaca
     4321 tcatcttcct gctgatgaac atcatcctgc tggtcatcgg tatgttcatg gacatcaccc
     4381 cggccatcct gattttcacc ccgattttcc tgcccatcgc caccagcttc ggcatgaccg
     4441 agatccagtt cggcgttatg ctgatcttca atatgtgctt gggcaacatc accccgccgg
     4501 tcggctcggt tctgtttgtc ggctgcggta tcggccatgt ctccatcgag caggtcacgt
     4561 ccaagctcat cccctacttt gtggcactgg tggccgccct gctggctgtc acctacattc
     4621 ccgcgctgtc cctgggcctg cccagcctga tgggcctgat ttgagcgcag cttaacagct
     4681 aactaaaata cgcagccccc gcagaaggtt gacctttctg cgggggctgc gctgttttta
     4741 gggagttata tacattgttt gcccgctctc cacggcctgg ggcagcccgg cggggaaggt
     4801 gcagcccacc gccagcgaga ccgtgcaccg ctctccgggc gtgagcagcg gcggcgcgat
     4861 ctccaacgga cgaacggtga aaaacttgtg gcaaacagcc ccttgcgttt ctgcccgcat
     4921 tgtgttatac tctacaccgt aaaactgaat acgacagcag gtgccttttt gcgcggttcc
     4981 gccgcacaga aaagggtaaa agggaagcgg gcgcaaatcc cgcacgatct cgtcactgtg
     5041 ataaggaagc ctgccgccag ggtgcgtttg caccggtcac tgggcatcag cccgggaagg
     5101 cggcggtggg tgttgatctt tcagccagga aacctgcctg ctgccgggta cgggggcgca
     5161 cagctctaaa tcacgaggaa ttggttgtac cgcaaagaac ctgctgaaag ataacaagtt
     5221 cgtggggagg ggctacctta accaggtggg gataacgga
//
LOCUS       DBMDSQ010000124         5241 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_3855_length_5241_cov_2.074903, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000124 DBMDSQ010000000
VERSION     DBMDSQ010000124.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 5241)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5241)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5241
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3855_length_5241_cov_2.074903"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(4..756)
                     /locus_tag="ACZPAX_05645"
     CDS             complement(4..756)
                     /locus_tag="ACZPAX_05645"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005934989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HIR1113415.1"
                     /translation="MTALQTIAVAFAMFSALPMPQFDWNAKNMRYALCAFPLVGAVCG
                     ALWCLCGMLPLPALARAAGFCLVPVWVTGGIHLDGYADTCDALSSYGDTAKKLEILKD
                     PHCGAFAVIRLCSYFAAYFALCGCVQFTPRVGALWALALVGERALSGLAVAAFPLAKN
                     TGLAHTFATAADRVNVRRALAVLCAAVAAALLALGGWALVLAAAGVFVRYYFVAKKQF
                     GGTTGDLAGWFLQKCEIWMLAALAACQWLGVL"
     gene            complement(756..1277)
                     /locus_tag="ACZPAX_05650"
     CDS             complement(756..1277)
                     /locus_tag="ACZPAX_05650"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005926375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0008820 - cobinamide phosphate
                     guanylyltransferase activity [Evidence IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HIR1113416.1"
                     /translation="MLTLVIGGAASGKSAFAESLCLRAPLPRTYLATMQVWDAECAAR
                     VAKHRAMRAEKQFDTVECPLHLDRLTLPRRGTVLLEDLGNLAANELYDPDGAGENAAA
                     AILHGIENIAQQCENLILVSNEVFSGGADYAGDTDRYLHTLAAVNNAAAARADAVVRV
                     VCGIPVYYKGSER"
     gene            complement(1271..2335)
                     /gene="cobT"
                     /locus_tag="ACZPAX_05655"
     CDS             complement(1271..2335)
                     /gene="cobT"
                     /locus_tag="ACZPAX_05655"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005934993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HIR1113417.1"
                     /translation="MTETELKNLLKTVAPPDEAARAAAHAHWAALAKPLGGLGQLETM
                     VEAAAALTGDPAPDFTRRAVLVLCADNGVVAQGVSQTNASVTRAVLENLTARRTSVCC
                     MAAAAHCEVVPVDMGIAGVPVPGAVDRRVAPGTRDFTQGPAMTREQAVQAVAAGIELV
                     RTQKQNGVRLLATGEMGIGNTTTSSAVAAVLLGRPAAEMTGRGAGLSDEGLHRKIHAI
                     ERGIAVNHPNAADPLGVLAALGGFDVAGLCGVFLGGALEKIPVIMDGFISGVAALCAV
                     RLCPAAAKAVFASHASSEPAARLVLDALHKKPLITADLHLGEGTGAVASLPLWDMALA
                     VYNHCYSFTEGGIAPYTPQC"
     gene            complement(2332..3678)
                     /locus_tag="ACZPAX_05660"
     CDS             complement(2332..3678)
                     /locus_tag="ACZPAX_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015538422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042242 - cobyrinic acid a,c-diamide
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HIR1113418.1"
                     /translation="MLQLVLAAPRSGSGKTTAACALLAAMQARGLAPCAFKSGPDYID
                     PMFHRAVLDVESHNLDLFFSKPETVRELYARHAAGHGAAVVEGAMGYYDGLGGVADTA
                     SAWHLADTLNLPALLVVRPKGASLTLAAELRGLMAFRTPHHIAGILLNDCKESLYKML
                     RPMLEKETGLPVVGCLPPLPEAAIESRHLGLKTAAEITDLQRKIALLADAAQRGIDWA
                     LLEKLCGRPAPAAIPQNFPAARVRIAVAQDAAFCFTYAETLEALRENGAELCFFSPLT
                     DAALPENIAGLYLPGGYPELYAAQLAANAPMRRAIKNAVESGLPTVAECGGFLYLGQT
                     LEDANGTPHQMAGVLPGQGIKVGRLMRFGYATLTARTDSMLFAAGEHIPAHEFHHWDS
                     TANGDALTAAKANGRQWACGFANAHIYAGFPHLYWAGTPLPQRFVCAAEHYIKERP"
     gene            complement(3680..4873)
                     /gene="cbiE"
                     /locus_tag="ACZPAX_05665"
     CDS             complement(3680..4873)
                     /gene="cbiE"
                     /locus_tag="ACZPAX_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015563724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046025 - precorrin-6Y
                     C5,15-methyltransferase (decarboxylating) activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046140 - corrin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6y C5,15-methyltransferase
                     (decarboxylating) subunit CbiE"
                     /protein_id="HIR1113419.1"
                     /translation="MRITLVGMGSGAPGGLTVRGLAALQGAGLIIGARRLLQNLPDGC
                     TDNRTALYKTDEICALLQEAGCERAAVVFSGDTGFYSGAGALCRALDAAGPPYTVEPG
                     VSSVQLLAAALGRPWQDWQLVSAHGCACDPVAACQKGVPTFFLTGGSETPATLCARLA
                     AAGYGEVIATVGENLGSDAQRLVTDTVAALAQQQFAPLSVLLAERCPAPPRRAPGLPD
                     EVFLRGKTPMTKQEVRAAVLAKLAVRPNDTLWDVGAGTGSVSVEMALAAPEGRVYAAE
                     CDADACELVCQNRAKFAVQNLHLTQGLAPEALADWPAPDAVFIGGSKGNLRAIVDAAL
                     AANPAARLCISAIALETLQQAVAALTAHGLTAQVTQIAVSRSRAAGSLHLLMANNPVF
                     LIARE"
     gene            complement(4870..>5241)
                     /locus_tag="ACZPAX_05670"
     CDS             complement(4870..>5241)
                     /locus_tag="ACZPAX_05670"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013977152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016994 - precorrin-6A reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6A/cobalt-precorrin-6A reductase"
                     /protein_id="HIR1113420.1"
                     /translation="DKPGNVLLTTGAKELPAFAVLDPARLYPRVLPTQAGITACEAAG
                     IPHRNILAMQGPFTQELNAALLRQFRISYMVTKDGGVAGGFAEKAEAAAQCGVQLIVL
                     RRPEEQGESTETILQRCREIL"
BASE COUNT          917 a         1806 c         1645 g          873 t
ORIGIN      
        1 catttacagc acccccagcc actggcaggc agccagcgcc gccagcatcc agatctcaca
       61 tttttgcaaa aaccacccgg ccaaatcgcc ggtggtgccg ccaaactgct ttttggcgac
      121 gaaataatac cgcacaaaca cgcccgccgc cgccagcacc agtgcccagc cgcccagggc
      181 cagcagcgcc gccgccaccg cagcgcacag caccgccagc gcacggcgca cattcaccct
      241 gtctgccgct gtggcaaagg tgtgggcaag gccggtgttt ttcgccagcg ggaacgccgc
      301 cacagccaag cccgacagcg cccgctcccc caccagcgcc agcgcccaga gcgccccgac
      361 gcgcggggta aactgcacac agccgcacag cgcaaaatac gctgcaaagt agctgcacag
      421 ccggatgacc gcgaacgccc cgcagtgtgg gtctttcaaa atttccagct tcttggccgt
      481 gtcgccgtag ctggacaggg catcgcaggt gtcagcgtag ccgtccaggt ggatgccgcc
      541 cgtgacccag accggcacca ggcagaagcc ggccgcgcgg gccagcgcgg gcagcggcag
      601 cataccgcac aggcaccaca gcgccccgca cacagccccc accagcggga aagcgcagag
      661 cgcgtaccgc atattttttg cgttccagtc aaattgcggc atcggcaggg cggaaaacat
      721 ggcaaacgcc acggcgatgg tctgcaaagc tgtcatcatc tttcgcttcc tttataatag
      781 accggtatcc cgcacacgac ccgcaccacg gcgtcggcgc gggcagccgc cgcgttgttg
      841 acggcggcca gcgtgtgcaa ataacggtcg gtatcgcccg cgtaatcggc cccgccgctg
      901 aatacttcat tggagacgag aattaaattt tcgcattgct gcgcaatatt ctcaatgccg
      961 tgcaaaatcg cagcggccgc gttttcccct gcaccgtctg ggtcgtacag ctcgttggcg
     1021 gccaggttac ccaggtcctc cagcagtacg gtcccgcgcc tgggcagggt cagccggtcg
     1081 aggtgcagcg ggcattccac cgtgtcaaac tgcttttcgg cccgcatggc gcggtgtttg
     1141 gcaacgcggg cggcgcactc ggcatcccac acctgcatcg tggcaaggta ggtgcggggc
     1201 agcggagcac gcaggcagag agactccgca aaggcgcttt tgccgctggc cgcaccgccg
     1261 atgaccagcg tcagcattgc ggggtgtacg gcgcaatgcc gccctccgtg aaggaatagc
     1321 agtggttgta gacggccagc gccatatccc acagcggcag gctggccacc gcgcccgtgc
     1381 cctcgcccag gtgcaaatct gcggtgatca gcggcttttt gtggagcgca tccagcacca
     1441 gccgggcggc gggttcgctg gaagcatggc tggcaaacac ggccttggcc gcagccgggc
     1501 acagccgcac cgcgcacagc gccgccacac cgctgatgaa gccgtccatg atgacaggga
     1561 ttttttccag cgcaccgccc aaaaacacgc cgcacaaccc ggcgacatcg aacccgccca
     1621 acgcagccag cacgcccagc gggtccgcgg cattggggtg gttgaccgca atgccgcgct
     1681 cgatcgcatg aatcttgcgg tgcagcccct cgtcggacag gcccgcgccg cgccctgtca
     1741 tctctgcagc cgggcggccc agcagcaccg ccgccaccgc gctggacgtc gtcgtgttgc
     1801 cgatgcccat ttccccagta gccagcagcc gcacgccgtt ctgcttctgg gtgcgcacca
     1861 attcaatacc cgccgcgaca gcctgtacag cctgttcgcg ggtcatagcc gggccttggg
     1921 taaagtcccg cgtgcccggg gccacgcggc gatccaccgc gccgggcaca ggtacacccg
     1981 caatgcccat gtccacaggg acgacctcgc aatgggccgc agccgccata cagcagacgc
     2041 tggtgcggcg ggcggtcagg ttttcgagca ccgcccgagt cacactggcg ttggtctggc
     2101 tgaccccctg cgccacaacg ccgttgtcgg cgcaaagcac cagcacggca cggcgggtga
     2161 aatcgggcgc ggggtcgccg gtcagcgcgg cggcagcctc gaccatcgtt tctaactggc
     2221 ccagtccgcc cagcggtttt gccagcgcgg cccagtgggc gtgtgcggcg gcgcgggcgg
     2281 cctcgtcggg cggggccacg gttttaagca ggttttttag ttccgtttcg gtcatggtcg
     2341 ttcctttata taatgttcgg cggcgcacac aaagcgctgc ggcagcggtg tccccgccca
     2401 gtacagatgt gggaatccag cgtagatatg tgcattggca aacccacacg cccattgccg
     2461 cccattagcc ttggcggcag tcagcgcgtc tccgttggca gtggaatccc agtggtgaaa
     2521 ctcgtgggcc gggatgtgct ctcccgccgc aaacagcata ctgtcagtgc gggcagtcaa
     2581 cgttgcgtag ccaaaccgca tgagccgtcc aactttaatc ccctgtccgg gcagcacgcc
     2641 cgccatttgg tggggcgtgc cgttggcatc ctccagcgtt tgccccagat agagaaagcc
     2701 gccgcactcg gccacggtgg gcaagccgct ctctactgcg ttttttatag cgcggcgcat
     2761 cggcgcgttg gcggccagct gggcggcgta cagttcgggg tagccgcccg gcaggtacag
     2821 cccagcgatg ttttccggca aggctgcatc tgtcagcggg ctgaaaaagc acagctccgc
     2881 gccgttttcc cgcaaggctt ccagcgtttc cgcataggtg aagcagaacg cggcatcctg
     2941 cgccacggcg atgcgcaccc gggcggcggg aaaattctgt ggtatggcgg ccggggcggg
     3001 gcggccgcag agcttttcca aaagcgccca atcgatgccg cgctgggcgg cgtctgccag
     3061 cagtgcaatt ttgcgctgca agtccgtaat ttcagcggcg gttttcagcc ccaaatggcg
     3121 gctctcaatg gcagcctcgg gcagcggcgg caggcagccc acaaccggca ggccggtctc
     3181 tttttccagc atggggcgca gcattttgta caggctttcc ttacagtcgt tgagcaaaat
     3241 tcctgcgata tggtgcggtg tgcgaaacgc catcagcccc cgcagctcgg ccgccagcgt
     3301 caggctggcc cctttaggcc gcacgacgag cagggcaggc agattcaacg tatccgccag
     3361 gtgccacgcg ctggccgtgt cggccacacc gcccaggccg tcatagtagc ccatggcccc
     3421 ctcgacgacc gccgcgccgt ggcccgccgc gtgccgggcg tagagttccc gcaccgtttc
     3481 cggttttgag aaaaacagat ccaggttgtg gctttccacg tccagcacag cgcggtggaa
     3541 catcgggtcg atgtagtccg gcccggattt gaaggcgcag ggagccagcc cgcgggcctg
     3601 catggctgcc aacaatgcac aggcagccgt tgtttttccg ctgccggagc gcggcgcggc
     3661 caggaccagt tgcagcatat tactccctcg cgatcaaaaa taccgggtta tttgccatca
     3721 gcagatgcag gctgcccgcc gccctgctgc ggctgacggc aatctgcgta acctgggcag
     3781 tcaggccatg ggcggtcagt gcggcgacgg cctgctgcaa agtttccagc gcaatggcgc
     3841 tgatgcagag ccgcgcggca gggttggccg ccagcgcagc gtccacgatg gcacgcagat
     3901 tgcccttgct cccgccgatg aacacggcgt ccggggcagg ccaatcggcc agggcttccg
     3961 gcgccagccc ttgggtaagg tgcaaatttt gcacggcaaa ttttgcgcgg ttctggcaaa
     4021 ccagctcaca cgcatcggcg tcacattcag cggcataaac gcggccctcg ggcgcagcca
     4081 gcgccatttc cacactgaca ctgccggtgc ccgcacccac atcccacagg gtatcgttcg
     4141 ggcgcacggc cagctttgcc agcacggcgg cgcggacctc ctgcttggtc atcggcgttt
     4201 tgccgcggag aaacacttca tccggcaggc cgggggcgcg gcgcggcggc gcggggcagc
     4261 gctccgccag cagcacgctc agcggcgcga actgctgctg cgccaacgcg gccactgtgt
     4321 cggtcacaag gcgctgggca tcgctgccga ggttttcccc cacggtcgct atgacctcgc
     4381 cgtacccggc tgctgccagc cgggcgcaga gtgtcgcggg cgtctcactg ccgcctgtca
     4441 ggaagaacgt tggcacgccc ttttggcacg cagctaccgg atcgcaggcg cagccgtggg
     4501 ccgatacgag ctgccaatcc tgccaggggc ggcccagtgc cgccgccagc agctgcacgc
     4561 tggacacgcc cggctccacc gtataggggg gacctgcggc gtccaatgca cggcagagtg
     4621 cccccgcccc ggagtaaaac ccggtgtccc cgctgaaaac gaccgccgcc cgctcgcagc
     4681 cggcctcttg cagcagtgcg caaatttcgt ccgttttgta gagggcggtt cggttgtcgg
     4741 tgcagccatc gggcaggttt tgcagcaggc ggcgtgcccc gatgatcagc cccgcgcctt
     4801 gcagtgcggc cagcccgcgc accgtcaggc ctccgggtgc cccactaccc atgcccacca
     4861 atgtgatcct cataaaatct ccctgcatcg ctgtaaaatt gtttcggtgc tctcgccctg
     4921 ctcctccggg cggcgcagca cgatgagctg tacgccgcac tgcgcagcgg cttcggcctt
     4981 ttcggcaaag ccgcctgcga ccccgccgtc cttcgtaacc atataggaga tgcggaactg
     5041 gcgcagcagc gcggcgttca gttcctgggt aaacggcccc tgcatcgcaa ggatattgcg
     5101 atgcgggatg cccgcagctt cgcacgcagt gatgcctgcc tgcgtcggca gcacccgggg
     5161 gtacagccgc gccgggtcca gcaccgcgaa ggcgggcagt tccttggccc cggtcgtcag
     5221 caggacattt ccgggtttgt c
//
LOCUS       DBMDSQ010000125         5201 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_3892_length_5201_cov_1.683529, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000125 DBMDSQ010000000
VERSION     DBMDSQ010000125.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 5201)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5201)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5201
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3892_length_5201_cov_1.683529"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(127..1491)
                     /locus_tag="ACZPAX_05675"
     CDS             complement(127..1491)
                     /locus_tag="ACZPAX_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014794869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA modification/repair radical SAM
                     protein"
                     /protein_id="HIR1113421.1"
                     /translation="MDLAEKLEILADSAKYDVACTSSGVDRPGRHGSLGSCAAAGICH
                     AFTPDGRCVSLLKVLYSNVCSYDCSYCVNRRTNDRPRATFTPRELADLTIGFYRRNYI
                     EGLFLSSAVLGTPDRTMELMIEALHILREEYHFNGYIHAKTIPGADPLLVEKIGRLAD
                     RLSVNIELPSETSLTALAPDKKKTAILRPMGQIAVQSAQSKKEMVLYRGAKPFAPAGQ
                     STQMIIGATPETDRHIMDLTEGLYKKYALRRVFYSAYLPVVADSRLPAPHTAPPLLRE
                     HRLYQADWLLRYYHFSARELLTEDEPNFDPYLDPKCTWAVRHPAFFPVEINTAAKEEL
                     LRVPGIGPKSALRIIQARRTQNLGLAELKRIGVVVKRAQYFITCKGRAAAHANRAEIA
                     NALLDPKAFSVGMQQLSLDDFVPKALPDAAPAVQKLAARGMAADVAAKTVRQEALQCL
                     TKRM"
     gene            complement(1655..2284)
                     /locus_tag="ACZPAX_05680"
     CDS             complement(1655..2284)
                     /locus_tag="ACZPAX_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007045978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family protein"
                     /protein_id="HIR1113422.1"
                     /translation="MFAERLKEARKAKRYSQAEVSKMLGVTQQAVGKWETGRSTPDPQ
                     TVARLAEILDTPSDVLLGLRKEPGVSAAVGRNAFSRYTESQIPVVGTVRAGYGALAFE
                     EDYGKEYACVKDPKNYFFLVVKGDSMEPRISDGDLALVHRQNTLDNGDLGVLVYGADG
                     EGTLKKYIQRGNSVVLQPFNPAYDEMVIKGEELDHLYIAGKVVETKAKW"
     gene            2534..2803
                     /locus_tag="ACZPAX_05685"
     CDS             2534..2803
                     /locus_tag="ACZPAX_05685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113423.1"
                     /translation="MNWELIKNKLCRLVATAAILLGGAMLSAAASGSMPLWAAILGLF
                     AAVIMLNAVCGILLPHPEESAGQPAPAPRKHPAVQLRVVRGGHAA"
     gene            2941..3687
                     /locus_tag="ACZPAX_05690"
     CDS             2941..3687
                     /locus_tag="ACZPAX_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016146259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HIR1113424.1"
                     /translation="MKLLEVCVDSLASARAAKQGGADRLEFCSALAVGGLTPYADLLR
                     QVKAEMSLPVRCLMRPRAGDFLYTKEEIDLLCHQIKTLHAAGADGFVIGALTPEGTLD
                     LPAMHTLLDACDGAPVTLHRCIDVSRDLRETYRAAAALGIDTVLTSGGAENCRKGASQ
                     LAGLLRLRDELHGPEVLIGAGVNAAIIDELRAALPGARAFHASCKTLLDSGMTFRREG
                     VPMGLPGLDEWHIQQTDAETVRAAKDAVMR"
     gene            complement(3893..4645)
                     /locus_tag="ACZPAX_05695"
     CDS             complement(3893..4645)
                     /locus_tag="ACZPAX_05695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HIR1113425.1"
                     /translation="MADRIHVGDKLPFFLYDTPYSAKNRFRDLLSQRAPLVLVFMANF
                     GHPITRTFASRYANTYAALQDGGFALVVRSRADKLSRSIGPNTLPYPLLCDAAGVLYE
                     HLNIPTSKSTLMSYSLEGWRILREAKKQGYKPTKGAEQQLPLTLILDRDGTVLFCHYG
                     ASLTDVPEDCAAIQSLLEELELTPEHREDAYEEELQAAMAAAPRPRSHHGRGAPETTD
                     EFQPVRPVREYNAPDLEKTSMFGLFDDPYDEN"
     gene            complement(4721..4914)
                     /gene="ssrS"
                     /locus_tag="ACZPAX_05700"
     ncRNA           complement(4721..4914)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACZPAX_05700"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(4930..5199)
                     /locus_tag="ACZPAX_05705"
     CDS             complement(4930..5199)
                     /locus_tag="ACZPAX_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007045983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HIR1113426.1"
                     /translation="MEDEDGKEVTFEVIDATEVNGTRYLAVIPYQEDPESLQEDAELI
                     LMRIGTDDEGEYMDIVDDDEELITVGKVFEERLRAMYDIDDSELQ"
BASE COUNT         1007 a         1568 c         1540 g         1086 t
ORIGIN      
        1 cgaacaggtt cagctggcct tcttcaggtg ggcaaccctc cgacggtatc tcgtgccggg
       61 cataactctc gaacacgcag cacaaaaagc ctgcaaaact tccgtcatag cggtaggcaa
      121 cgtcggtcac attcgcttcg taaggcattg cagtgcctcc tgccgcacgg ttttggcggc
      181 cacgtcagcc gccataccgc gggcggccag tttttgcaca gccggggctg catccggtag
      241 ggccttgggt acaaaatcgt ccagcgaaag ctgctgcatc cctacgctga acgccttggg
      301 gtcgagcagg gcgttggcaa tttccgcacg gtttgcgtga gctgctgccc ggcccttgca
      361 ggtgatgaaa tactgcgccc gcttgaccac cacgccaatg cgcttgagtt cggccaagcc
      421 cagattctgc gtgcgccgcg cctgaatgat acgcagggcg cttttggggc cgatgccagg
      481 cacccgcagc agctcctcct tcgcggcagt attgatctcc actgggaaaa acgccgggtg
      541 ccgcacggcc caggtacatt tgggatcgag gtaggggtca aaattcggct catcctcggt
      601 cagcagttcc cgcgccgaaa aatggtagta ccgcagcagc caatccgcct ggtatagcct
      661 gtgctcccgc agcaggggcg gggcggtgtg cggcgcgggc agacggctgt ccgctaccac
      721 tggcagatag gccgagtaaa acacacgccg cagcgcatat tttttgtata gcccttctgt
      781 caggtccatg atatgccggt cagtttccgg cgtagcgcca atgatcatct gggtgctctg
      841 cccggccggg gcaaagggct tcgcgccccg gtagagcacc atctcttttt tgctctgggc
      901 gctctgcacc gcgatctggc ccatggggcg caggatggcg gtctttttct tgtctggggc
      961 cagcgccgtc aagctcgtct cgctaggcag ttcgatattg accgagaggc ggtctgccag
     1021 ccgcccgatc ttctccacca gcagcgggtc cgcgccgggg atcgtcttgg cgtggatgta
     1081 gccgttgaag tggtattcct cccgcaggat gtgcagtgcc tcgatcatca gctccatcgt
     1141 gcggtcgggc gtacctagca ccgcgctgga caaaaacagc ccctcaatat aattgcggcg
     1201 gtaaaacccg atagtcaaat cggccagctc ccgcggggtg aaagttgccc ggggccggtc
     1261 gttggtacgg cggttgacac agtagctgca atcatagctg catacgttgg agtacagcac
     1321 cttcagcagg gaaacgcatc gcccgtctgg cgtaaaggcg tggcagatgc ccgccgccgc
     1381 acagctgccc aggctgccat gccgccccgg acggtcaacg ccgctggacg tgcaggccac
     1441 atcatatttg gcgctgtccg ccagaatttc cagtttttcc gcaaggtcca tcgcacctgt
     1501 ctcctaaaaa agggtataaa aaatacagcc cgccgcatat ccttgttttg caaggtgtgc
     1561 cgcgggcagc atttttgtga aataattcaa tttctacatt gaaagtccaa attataggac
     1621 ttcgcttatg ctacaataga atcgtgaaag ggctttacca ctttgccttg gtttccacga
     1681 ccttgccggc gatgtagagg tgatccaatt cctcaccctt gatgaccatt tcgtcatagg
     1741 ccgggttgaa aggctgtagc acgaccgagt tgccgcgctg gatatacttt ttcagcgtac
     1801 cctcgccgtc tgcgccatag accagcacac ccaggtcgcc gttgtccaac gtgttctgac
     1861 ggtgaacgag ggccaggtcg ccgtcggaaa tgcgcggctc catactgtcg cccttgacca
     1921 cgaggaagaa gtagttcttc ggatccttga cgcaggcgta ctccttgccg tagtcctcct
     1981 caaaggcgag ggccccatac ccggcgcgga cggtgcccac caccgggatc tgggattcgg
     2041 tatagcggct gaaggcgttg cggcccacgg cagcggaaac gcccggctcc ttacgcaggc
     2101 ccagcagcac atcggagggg gtatccaaaa tttctgccag acgcgcgacg gtctgtgggt
     2161 ccggggtgga acgcccggtc tcccacttgc cgacagcctg ctgcgtcaca cccagcattt
     2221 tggaaacctc ggcctggctg taccgtttgg ccttgcgggc ctctttcaag cgctctgcaa
     2281 acatcggaaa tctccttttt atagtagggt aaaccacatc ttgtatgcgg ctctgattct
     2341 ttagtctcat tatacaactg tccgtggttt ttgtaaagag ttttttgcaa acattttcaa
     2401 aaataagttg taaataggca ttgacaacaa ccgtctggtg tattatgata agagcaacga
     2461 acaaaacgct gttgtccgtt gttaaaattg cggcagtcgg ccacgcgggg ccgactgatt
     2521 tggaggtatc ggtatgaact gggaacttat caaaaacaag ctgtgccgtc ttgtcgccac
     2581 ggcggctatc ctgttgggag gggctatgct cagtgctgcc gcctccggct ccatgccgct
     2641 ttgggcggcc attctggggc tttttgcggc ggtcatcatg ctcaacgccg tctgcggcat
     2701 cctcctgccc cacccggagg aatcggccgg ccagcctgcc cccgcgccgc gcaaacaccc
     2761 tgcggtacaa ctgcgcgtcg tgcgcggcgg ccacgcggcg taaaactctg cctactccta
     2821 ttcgttgtga tacacatata actgcggcaa gccccgcgca gctttccggc tgtgcggggc
     2881 ttgccgtatc caaaatttca tgctacaatg aggaaaacgc caaaggggat gtgaccacac
     2941 atgaagttgt tggaagtctg tgttgacagc ctggcctccg cccgcgccgc caagcagggc
     3001 ggggccgacc ggctggaatt ttgcagtgcg ctggccgtgg gcggtctgac gccctacgcc
     3061 gaccttttgc ggcaggtcaa ggccgaaatg tccttgccag tgcgctgcct gatgcgcccc
     3121 cgtgccgggg attttctcta cacaaaagag gagatcgacc tgctctgcca tcaaatcaag
     3181 acgctgcatg ccgccggggc ggacggtttc gtcatcggcg cactgacacc ggagggcacg
     3241 ctggatctgc ccgccatgca cacgctgctg gatgcctgcg acggtgcgcc ggtcacatta
     3301 catcgctgca tcgatgtttc ccgcgatttg cgcgaaacct accgtgccgc ggcggccctt
     3361 ggcatcgaca cagtgctgac cagcggcggg gccgagaact gccgcaaggg tgcatcccag
     3421 ctggccgggc tgctgcgcct gcgggacgag ctgcacggcc cggaagtgct cattggcgcg
     3481 ggcgtcaacg ctgctatcat cgacgaactg cgggctgcgc tgcccggggc gcgtgctttc
     3541 cacgccagct gcaaaacgct gttggacagc ggcatgacct tccgccgcga gggcgtaccc
     3601 atgggcctgc ccggcctgga cgagtggcac atccagcaga ctgacgccga gaccgtgaga
     3661 gcggcgaagg atgcggtgat gcggtgattt tccgagcctt ctgccgaggg agaaggtggc
     3721 cgaaggccgg atgaggggca ggttcacgca gcagcctctt ttaacgggcg atagcggcaa
     3781 agccgccccc ccatcagtcc gcttagcgga cagcttccca cgagagggaa gccattttat
     3841 tcagtcaaaa agacaaaccc cgcctgccga ttgtttcatc ggcagacggg gtctaatttt
     3901 catcataagg gtcgtcaaac agtccgaaca tcgaggtttt ctccagatca ggcgcgttgt
     3961 actcccgcac ggggcgcaca ggctggaact cgtcggtcgt ctcgggggcg ccgcggccat
     4021 ggtgggagcg ggggcggggg gccgccgcca tggcggcctg cagttcctcc tcatacgcat
     4081 cctccctgtg ttcgggtgtc agttccagtt cctccaacag gctctggatg gccgcgcaat
     4141 cctcgggcac gtcggtcagg cttgcgccgt agtggcagaa cagcaccgtg ccgtcgcgat
     4201 ccagaatcag tgtcagcggc agctgctgtt ctgcgccttt tgtgggcttg tagccctgct
     4261 ttttcgcttc gcgcaggatg cgccagcctt ccagcgagta gctcatcagg gtgcttttgc
     4321 tcgtggggat gttcagatgc tcgtacaaaa caccggccgc gtcgcagagc aggggatagg
     4381 gcagggtgtt cggcccgata ctgcgggaga gcttgtccgc gcgggagcgc accaccagcg
     4441 caaagccgcc gtcctgcaag gctgcgtatg tattggcgta gcggctggca aaggtgcggg
     4501 tgatggggtg gccgaagttt gccataaaca ccagcaccag cggcgcgcgc tgcgaaagca
     4561 ggtcgcggaa gcggttcttc gcgctgtacg gtgtatcgta caaaaagaag gggagcttgt
     4621 ccccgacgtg aatcctatcc gccatggtca aacacccctt tcccaaaaat tataacccaa
     4681 aaaaccggac aggaaaagcc ctgcccggga tgtgtgctgt aagaaccccg cacagccgtc
     4741 tgcagccaat caaaacctac cggtataggc aggtgggtat cctacggggg cgttcacgct
     4801 cccttcttca gcccaaaggc gggaaggttt gcaatccagt cccagacccg gattccctaa
     4861 atttgattag taggatttta taaggccgca acaaatcgaa ccgcgcaggg tgtgaggcct
     4921 tgcagcctct tactgcaatt cagagtcgtc gatgtcgtac atcgcacgca ggcgctcctc
     4981 gaacaccttg ccgacggtga tcagttcctc atcgtcgtcc acgatgtcca tatattcgcc
     5041 ctcgtcgtcg gtgccaatgc gcatcaaaat cagctcggcg tcctcctgca ggctctccgg
     5101 atcctcctgg taggggatga cagccagata gcgggtgccg ttgacttcgg tagcatcgat
     5161 cacttcaaac gtgacctcct tgccatcctc atcttccagc g
//
LOCUS       DBMDSQ010000126         5200 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_3894_length_5200_cov_2.035889, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000126 DBMDSQ010000000
VERSION     DBMDSQ010000126.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 5200)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5200)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5200
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3894_length_5200_cov_2.035889"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..443
                     /locus_tag="ACZPAX_05710"
     CDS             <1..443
                     /locus_tag="ACZPAX_05710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113427.1"
                     /translation="YLEGRLHEDDFVAHRLFWRCDKVVCVDKPLYHYRLRNGSICRTN
                     IKPEAFDAVDGLADRYRFYVENRADRTVVDAAYAACWRRYLFLCAKVRQAPTPELVKA
                     ISKEQFLMQGLISYLPNCRNMKMTEKLSAARWAMMSAQSLCPIK"
     gene            565..>1670
                     /locus_tag="ACZPAX_05715"
     CDS             565..>1670
                     /locus_tag="ACZPAX_05715"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR1113428.1"
                     /translation="MALRNHDKEKRPRVLLIPPPDLPVPAVQGGAVETLLTHLIRENE
                     KQGQLELLCASVPDDVARRAAEGWQHTKMLYVPRPSGHRRYWPMVFLERCVGIAAPYD
                     PWYQKVQLALALELPPPDLIIGEGGNLTQCSAISRMYGRRRCLAHLHGQTNCTPVMDD
                     IYGGVLAISEFIRGEYLSTSSIDHQRAYILHNCIDTNRFRPGPASKTLRAQLGFTDDD
                     FIVLYCGRLDPDKGIHKLMEAVASLPLPHVKLLIVGSPFFARTQQSVFQRKLEQQAKS
                     LGDRVQFTGYIPNEDLPDYYRLADLCCVPTLVEEAAGLVAVEAMACGRPVLATRSGGM
                     PEYLEGSQAVLVERGDTVADQLAWSIRMLYEHPAL"
     assembly_gap    1671..1680
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1786..2847
                     /locus_tag="ACZPAX_05720"
     CDS             1786..2847
                     /locus_tag="ACZPAX_05720"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR1113429.1"
                     /translation="MSKPTITVIVPVYKAMATLDRCVESVVSQDVAGGLACILVDDGS
                     PDDSGKLCDAWAARDPRVTVIHQDDLGVSGARNSGLAAADSEYIVFLDSDDALRPGAL
                     QAALDAQNAAPNSFVCWHYTTDENDTTPVTAAAETRPQSGLARLWLDCLLAMPWNKLY
                     RTAYAQQVQFDRQYTLGEDLQFVLDYVELLGKITPDFAYTALTAPLTFYDCSRGGTLS
                     TRYHADYCKIWPEHFAKLNRACNAAHCPQEDMRPLHRAELTVFAEGVADILRRDPAKS
                     ARVRRDKAIAALRSPWMHALLERMKIEGAYSAYYLPCRWHSTRLIWALAEAKRTGSPL
                     YGKLDWAGYYLLRGKMRGD"
     gene            3089..3685
                     /locus_tag="ACZPAX_05725"
     CDS             3089..3685
                     /locus_tag="ACZPAX_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014354890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop nucleotide/nucleoside kinase family
                     protein"
                     /protein_id="HIR1113430.1"
                     /translation="MDKKLILVTSPPACGKTYVAKALAHELQHIVYLDKDTLIVLSKQ
                     IFKVAGQPYDRSSAFFQANIRDYEYDCVLALAMEALDYDDIVLINAPFTQEVRDNAFI
                     ANLKAKLAEKGATLAVIWVETSPEVVHQRMIDRNSDRDTWKLTHWDEYIKQCNFAIPE
                     NLADPQHKDNLILFQNNNDEEFAASMKKCVAILEDAEN"
     gene            complement(3737..4309)
                     /locus_tag="ACZPAX_05730"
     CDS             complement(3737..4309)
                     /locus_tag="ACZPAX_05730"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015558621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HIR1113431.1"
                     /translation="MSDKKIIIDVVVDVQRDFITGALANEQAQANVAHLAKVAKAHAE
                     AGHWMFFTRDTHEDNYLDTREGRHLPVEHCVEGEDGWNFAPELEDAMAATEPEVTHIV
                     NKPTFGSYMLPSDILDELMAHNKAATDIEKFVIYGYCTDICVISNAMVLRAAFPETEI
                     EIQSDCCAGVTPRSHQTALDAMRNCQFTIV"
     gene            4704..4780
                     /locus_tag="ACZPAX_05735"
     tRNA            4704..4780
                     /locus_tag="ACZPAX_05735"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4738..4740,aa:Arg,seq:ccg)
     gene            complement(5011..>5200)
                     /locus_tag="ACZPAX_05740"
     CDS             complement(5011..>5200)
                     /locus_tag="ACZPAX_05740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113432.1"
                     /translation="GHNTEKPGRLILKIDSVPADKKATFSTEEIRLMRITINRLRNER
                     LAKGQYTDAADDMLLKLF"
BASE COUNT         1077 a         1519 c         1444 g         1150 t
ORIGIN      
        1 actacctcga aggccgcctg catgaggatg attttgtggc gcaccgcctg ttctggcgct
       61 gcgataaggt cgtctgtgtg gacaagccgc tctaccatta ccgcctgcgc aacggcagca
      121 tctgccgcac caacatcaaa cccgaagcct ttgacgctgt ggacggtctg gccgaccgtt
      181 accgcttcta tgtggagaac cgcgctgacc gcacggtcgt ggatgccgcc tatgcggcct
      241 gctggcggcg gtatctgttc ctctgcgcaa aggtacgtca ggccccgacg cccgagctgg
      301 tcaaggccat cagcaaggag cagttcctga tgcagggctt aatcagctac ctgcccaact
      361 gccgcaacat gaagatgacc gaaaagctca gcgccgcccg ctgggccatg atgagcgcac
      421 aaagtttgtg cccgatcaag taaggttcgc gcggccctca acctgccggg aactgctttc
      481 ctgcgggaaa gctgcgggtc gggcataccc ggcccctaca cgcagcctat atctgttcaa
      541 aatccacttt ctaaggagcc tgcattggcg ttacgaaatc acgacaaaga aaagcgtccc
      601 cgcgtgctac tgataccgcc gccggatctg ccggtgcctg cggtacaggg cggtgcggtg
      661 gaaaccctgc tgacccatct gatccgtgaa aacgagaaac agggtcagct ggagcttttg
      721 tgcgccagtg tgcccgatga tgtcgcccgc cgcgctgccg agggctggca acacaccaaa
      781 atgctttacg tgccgcgccc cagcgggcac cgccgctact ggccaatggt ctttctggag
      841 cggtgtgtcg gcatcgccgc cccctatgac ccctggtatc aaaaagtcca gcttgccctc
      901 gcactggaat tgcccccgcc cgacctgatt atcggcgagg gcggcaacct gacccaatgc
      961 agcgccatca gccgtatgta tggccgcagg cgctgcctgg cgcacctgca cggccaaaca
     1021 aactgcaccc cggtcatgga tgatatctat ggcggcgtgc tggccatcag cgagttcatc
     1081 cgcggcgagt acctttccac cagcagcatt gaccaccagc gcgcctacat tctgcataac
     1141 tgcattgata cgaaccgctt tcgccccggc ccggcgtcca aaacgctgcg tgcccagctg
     1201 ggctttaccg atgatgattt catcgtcctt tactgcggcc gccttgaccc ggacaagggc
     1261 atccacaaat tgatggaggc cgtcgcgtcg ctgccgctgc cccacgtcaa actgctgatc
     1321 gtgggcagcc ccttctttgc ccgcacccag caaagtgtgt tccaacgcaa attggagcag
     1381 caggccaaga gtctgggcga cagggtgcag ttcaccggtt atatccctaa tgaagattta
     1441 ccagactatt accgcctcgc tgacctgtgc tgcgtgccca ccctggtgga agaagccgca
     1501 ggtcttgtag cggtggaggc catggcctgc ggccgccctg tgcttgccac ccgcagcggc
     1561 ggtatgcccg agtatctgga aggcagccag gccgtgcttg tcgagcgcgg cgacaccgtg
     1621 gccgaccagc tggcgtggag tatccgtatg ctgtacgagc acccggccct nnnnnnnnnn
     1681 gatgggcgcg gccggggccg agcgcgcaaa ggcgttctcg gtagaacatt actatgcgga
     1741 atttgtgcgc atcgtgcgcg acatgactga aaacggaggt gcccagtgag caagccgacc
     1801 atcaccgtca ttgtgccagt ctacaaggcc atggctacgc tggaccgctg cgtcgagagc
     1861 gttgtctccc aggacgtggc aggcgggctg gcctgcatcc tggtggacga cggcagccct
     1921 gacgacagcg gcaaactgtg cgacgcctgg gccgcgcgtg acccccgcgt gacggtcatc
     1981 catcaggacg acctgggcgt ttccggtgcg cgcaacagcg gtctggccgc agcggacagc
     2041 gagtatatcg tctttcttga ctcggacgat gccctgcgcc ccggggcttt gcaggcggcc
     2101 ctggacgccc agaacgccgc accgaattct ttcgtctgct ggcactacac caccgatgag
     2161 aacgacacta cgccggtaac agccgccgcc gaaacccgcc cccagagcgg cctggcccga
     2221 ctgtggctgg actgcctgct ggcgatgccc tggaataaac tctaccgcac ggcctatgcc
     2281 caacaggtac agtttgacag acagtatact ttgggcgaag atttacagtt cgtattggat
     2341 tatgtcgaac ttttgggcaa aatcaccccg gattttgcct atacggccct gaccgcaccg
     2401 ctgacttttt acgattgcag ccgcggcggg acactttcga cccgatatca cgcagattac
     2461 tgcaagattt ggcccgagca ttttgccaag ctgaaccgtg cttgcaacgc cgcccactgc
     2521 ccacaggagg atatgcgccc cctgcaccgt gccgagctga ccgtttttgc cgagggcgtc
     2581 gccgacatcc tgcgccgtga cccggccaag agtgcccgcg tgcgccggga taaggccatc
     2641 gccgcgctgc gcagcccctg gatgcacgct ttgctggaac gcatgaaaat tgagggagcc
     2701 tattcggcct actacctccc ctgccgctgg cacagcaccc gcctgatctg ggcgctggcc
     2761 gaggccaagc gcaccggttc acccctgtac ggcaagctgg attgggctgg ctattacctg
     2821 ctgcgcggaa aaatgcgcgg ggattaaacg taaaaccgcg gtgcctggcc gcggtttttg
     2881 tgttataagg gctgtgacgc gaatcctcta tgtcgcgcac aaaaatccat atttgcactt
     2941 tcgttctcct catgctcccg cccttttctt gatacacttt gcaaggtttg tcctttttgg
     3001 aaacgtttcc tattgaaatt gttaaatgtt tgtcgtacaa tacgtttata cgctaagcgt
     3061 ataacgtttg tatagagaag ggttctccat ggataaaaaa ctgatcctcg taacctctcc
     3121 gcccgcctgc ggcaaaacct acgttgccaa ggcgctggct cacgaattgc agcatatcgt
     3181 ctacctggat aaggacacgc tgatcgtctt gtccaaacag atttttaagg tagccgggca
     3241 gccgtatgac cgcagcagcg cgtttttcca ggccaacatc cgcgattatg agtatgactg
     3301 cgtcctggcc ctggccatgg aggcgctgga ctatgacgat atcgtactca ttaacgcgcc
     3361 gttcactcaa gaagtgcgcg acaatgcgtt catagccaat ctgaaggcca aactggctga
     3421 gaaaggtgcg acgctggccg tcatctgggt cgagacctcg cccgaggtcg tccaccagcg
     3481 gatgatcgac cgcaactccg accgcgacac ctggaagctg acccattggg acgagtacat
     3541 taagcagtgt aattttgcca tcccggaaaa cctggccgac ccccagcaca aagataactt
     3601 gattttgttc cagaacaaca acgacgagga atttgccgcc tctatgaaaa agtgcgtggc
     3661 gattctcgag gacgccgaga actgaaaaac gccggtacag cacttacgct gtaccggcat
     3721 ttttgtgatg taaactttaa acgatggtga actggcagtt ccgcatggcg tctagcgcgg
     3781 tctggtgcga ccggggcgtg accccggcgc agcaatcaga ctgaatctcg atttccgttt
     3841 ctgggaaggc cgcgcgcagc accatcgcgt tgctgatgac gcagatgtcg gtgcagtagc
     3901 cgtagatgac gaatttttca atatcggtcg cggccttgtt gtgggccatc agctcgtcca
     3961 gaatgtcgct gggcagcata tagctcccga atgtcggctt attgacaata tgggtgactt
     4021 ccggctcggt cgcggccatg gcgtcctcca actcgggcgc aaagttccag ccgtcctcgc
     4081 cctcgacgca gtgttccacc ggcaggtggc ggccctcccg cgtgtccaga tagttgtcct
     4141 cgtgcgtgtc acgggtgaaa aacatccaat gccccgcctc agcgtgcgcc ttggcaacct
     4201 tcgccaaatg cgccacattg gcctgcgcct gctcgttcgc caacgcccca gtgataaagt
     4261 ccctctgcac atcgactacc acgtcgatga tgattttttt gtcgctcata gctacaactt
     4321 cctctctgcg tttgcaacca gtatactgca atatgtgcgg gatgtaaagg gctgcggtga
     4381 gcatgggacg ggcggatatt tgggcgattt tttatgagaa gaagaaaaat agttgaagaa
     4441 aacatttgca tttttctgtg agatttgcta tactatataa agccaattat gtaagccata
     4501 agtaatcgtg gcgcggttgg atagtgcgtt gggctccgac agctgcgaca gggcaggttt
     4561 caggctcgct tttgtgcgat acagcgttat ttgcgttcgc aaaaatatgg tggtcaacgg
     4621 tagtcagacc acgaattgac cactattgga aattgactac tttacaagat tgaaatacgc
     4681 tgtaacgcgt ttttcataca taagcgatcg tagctcagct ggatagagcg ttcggctccg
     4741 acccggaagg ccagaggttc gaatcctccc ggtcgcacca agagcagcac cttatggggt
     4801 gctgctcttt ttgttttgca cggattttat ggggcagcaa aagggagaat tcgaacagcc
     4861 cgtgccccgc gctttgcgtc ccgcggggca gaaacgcccc ggtggggcgt ttctagggcg
     4921 cggcttggcg aattccttcg ggcacactta aaaacctcat tgcaaagtgt tttttgcaat
     4981 gaggtttttg tttttattag tgtttgattt tcagaataac tttagcagca tatcatctgc
     5041 ggcatcggtg tattgacctt ttgctagacg ctcgttcctc agcctgttta tagtgattcg
     5101 catgaggcgg atttcttctg tagaaaaagt agctttttta tcggctggga cagaatcaat
     5161 tttcagtatc agtcgccccg gtttttccgt gttatggcca
//
LOCUS       DBMDSQ010000127         5177 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_3912_length_5177_cov_1.794326, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000127 DBMDSQ010000000
VERSION     DBMDSQ010000127.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 5177)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5177)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5177
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3912_length_5177_cov_1.794326"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..505)
                     /locus_tag="ACZPAX_05745"
     CDS             complement(<1..505)
                     /locus_tag="ACZPAX_05745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113433.1"
                     /translation="MKQKTKDIIGALLLGLLCTLALSAVITVADTETARTDLMIGMQY
                     ETTGLDLDAAPGLLVGLVKLHQNFPLDAWAWGPAAAGLAALLYWVRRQRTAKPRCAEL
                     VLSIVFGVAEVLGLSICKLGSWAFVFKNPYQVFVGAVCMAGYGVLFYHAVWGLYALLG
                     RPGGAEAE"
     gene            complement(511..1194)
                     /locus_tag="ACZPAX_05750"
     CDS             complement(511..1194)
                     /locus_tag="ACZPAX_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HIR1113434.1"
                     /translation="MAIFAIGDMHLSLGTDKPMDVFPGWEGYLPRLEANWRKLICPED
                     TVVLAGDTSWAMNLNDTKADFAFIQNLPGQKWLLKGNHDYWWTTTRKMETFLAANGFD
                     TLHILHNNACIVGDVALCGTRGWPFDDVAAQGEKLMAREAGRLRMSLQAAGETREKIA
                     FLHYPPIYPGAQAQELVDVLNEFHVTECCYGHLHGKSIRYAMQGERDGITYRLISADG
                     LSFCPYKIR"
     gene            complement(1222..2670)
                     /gene="thrC"
                     /locus_tag="ACZPAX_05755"
     CDS             complement(1222..2670)
                     /gene="thrC"
                     /locus_tag="ACZPAX_05755"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HIR1113435.1"
                     /translation="MQYLSTRDSALRVSAQEAIVRGLAPQSGLFVPETFPKADLEGWK
                     DLSYPVLAEKVLAGFLTEYSADFLHTATAATYGEAFGGKAGETVKVRDGLYSLELWHG
                     PTCAFKDYALQLMPKLLVEAKKMLGRTETTRILVATSGDTGKAALAGYADLDGIEMEV
                     FYPNDGTSEIQHLQMATQKGQNVQVYAVQGNFDDAQTGVKKVFADADVAAELEKRGIR
                     LSSANSINWGRLVPQIVYYFYAYFRLAERGAVAWGKPVDFCVPTGNFGDILAGYYAKQ
                     MGLPVGKLVCASNKNNVLTDFLRTGTYDAHRTFYKTTSPSMDILISSNLERLLYHVSG
                     SSEKVAAWMQELSQTGKYTVDTETLAKIQASFAAGYADDAEGAAEIKARFDEDNYLCD
                     THTAVAFRVAEALRTEAPMVVLSTASPFKFPRDVLTALGETAPASDFAAMAALTAKTG
                     AEAPASLSELDKLEVRFKTVLQPADIRTASLR"
     gene            complement(2723..3073)
                     /locus_tag="ACZPAX_05760"
     CDS             complement(2723..3073)
                     /locus_tag="ACZPAX_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015538547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HIR1113436.1"
                     /translation="MTDTGHIGEAIAAKYYQQRGWLLLNHNYRTRMGEIDLILYKDDT
                     LVFAEVKTRTGAMLDRPAAAVDARKQQRLILAAQQYLQCSPYRDAAIRFDVVEVTPAA
                     KGWQVHCIPSAFEC"
     gene            complement(3066..3710)
                     /gene="rnhB"
                     /locus_tag="ACZPAX_05765"
     CDS             complement(3066..3710)
                     /gene="rnhB"
                     /locus_tag="ACZPAX_05765"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015564083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HIR1113437.1"
                     /translation="MPRTTGTTTSSNEALYAFDAAKRAEHGVLCGVDEAGRGPLCGPV
                     CCAAVILNPDDPIAGVNDSKKLTEKRREALFEEITNRALAYKIVFISPQEIDERNILW
                     ATMDGMAQAVAGLEITPDYVLIDGNRCPPGLIQPAEAVVKGDANSASIAAASILAKVS
                     RDRYMLELDKQYPQYQLAKHKGYPTKLHYELIAQYGIQPFYRRSFLKKQGYWHD"
     gene            complement(3676..4623)
                     /gene="ylqF"
                     /locus_tag="ACZPAX_05770"
     CDS             complement(3676..4623)
                     /gene="ylqF"
                     /locus_tag="ACZPAX_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HIR1113438.1"
                     /translation="MSQSDIINSRQIQWFPGHMTKTLRLMEKEIRNVDCVLQILDARI
                     PLSSLNPEIERITAGKPHLYVLNKSDLADPEITKQWLAYFKSAGAGCIAMDSKQRGRA
                     TATKGLIEKELSALMERRRNRGMVGAAIRVMIVGIPNVGKSTFINSFAGTTRAKAANK
                     PGVTRGKQWISVDNFDLMDMPGVLWKKFDSLETASNLAFIGSIRDDILDIEELACGLL
                     SSVRGIYPQQLLARYKLDAATLELPPYDLLQAIGAKRGMLISGGEVDTERAAKMLVGE
                     FRASKWGRLSLERPPQRAIQEEAEEVIDDAPDDWDDDEQ"
     gene            complement(4620..5177)
                     /gene="lepB"
                     /locus_tag="ACZPAX_05775"
     CDS             complement(4620..5177)
                     /gene="lepB"
                     /locus_tag="ACZPAX_05775"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HIR1113439.1"
                     /translation="MGAAFDESFKKRYRRNKEALEWYDALAMAVAVIAIVFTFFVRIV
                     KVDGHSMDPTLFNGERILINLMKQPDYGDIVVVDGYTSYGKPLVKRVIGKGGDTINID
                     FAAGIVYRNGVALDEPYTAEPTYLYESVDFPITVPDGCLFVMGDNRNNSTDSRDSRVG
                     CVDERDIMGAAMLRILPFGKIGAAE"
BASE COUNT          946 a         1579 c         1579 g         1073 t
ORIGIN      
        1 gttctgcttc tgccccgcca ggccgcccca gcagggcgta caagccccag acagcgtggt
       61 agaacaacac cccatacccg gccatgcaga cagcgcccac aaagacctga tagggatttt
      121 taaagacgaa ggcccagctg cccagcttgc agatgctcag gcccagcacc tcggcgacac
      181 caaagacgat gctcagcacc agttcggcgc atctgggctt ggccgtgcgc tggcggcgca
      241 cccagtagag cagcgcggcc agccccgccg ccgcaggccc ccaagcccag gcgtccagcg
      301 ggaagttctg gtgcagcttg acaagcccca ccagcagccc cggcgcggcg tccaggtcaa
      361 ggccggtcgt ctcgtactgc ataccgatca tcaggtcggt gcgagcggtt tcggtatccg
      421 ccaccgtgat gacggcggag agcgccaacg tgcagagcag ccccagcagc agcgccccga
      481 tgatatcttt tgttttctgc ttcataggct tcaccgtatt ttataggggc agaacgacag
      541 gccatcggcg gagatcagac ggtaggtgat gccgtcccgc tcgccctgca tggcatagcg
      601 gatggacttg ccgtgcaggt ggccgtagca gcattcggtc acatggaact cgttcagcac
      661 atccacaagc tcctgcgcct gtgcgcccgg gtagatgggc gggtagtgca ggaaggcgat
      721 tttttcacgc gtctcccccg ccgcctgcaa actcatccgc aggcggccag cctcgcgcgc
      781 catgagcttt tccccctggg cggccacatc gtcaaagggc cagccccggg tgccgcacag
      841 cgccacatcg cccacgatgc aggcgttatt gtgcagaatg tgcagcgtgt caaagccgtt
      901 ggcggccaaa aacgtctcca tcttgcgggt cgtcgtccac cagtagtcgt ggttgccttt
      961 cagcagccat ttctggccag gcaggttttg gataaaggca aaatccgcct tggtgtcgtt
     1021 caggttcatg gcccagctgg tgtcgcccgc caggacaacg gtgtcctccg ggcatatgag
     1081 cttgcgccag ttggcttcca gtcggggcag gtacccctcc caccccggga aaacgtccat
     1141 gggcttgtcc gttcccagcg agaggtgcat atccccgatt gcaaagatgg ccatacggcc
     1201 ctcactttct agttgttgtt tttaacgaag ggatgcggtg cggatatccg caggctgcaa
     1261 gaccgtcttg aagcgcactt ccagcttgtc cagctcactt aaggacgccg gggcctcggc
     1321 gccggtcttg gcggtcaagg ccgccatagc ggcaaagtcg ctggcgggag cggtctcgcc
     1381 cagagcggtc agcacgtcgc gcgggaactt gaaggggctt gccgtggaca gcacgaccat
     1441 gggagcctcg gtgcgcaggg cttcggccac gcggaacgcc acggcggtgt gggtgtcgca
     1501 gaggtagttg tcctcgtcaa agcgggcctt gatttccgct gcgccctcgg cgtcatccgc
     1561 atagcccgcg gcgaagctcg cctggatctt cgccagcgtc tcggtatcga cggtgtactt
     1621 gccggtctgg ctgagctcct gcatccaggc ggcgaccttc tcactgctgc cgctgacgtg
     1681 gtagagcaga cgctcgaggt tcgaggaaat caggatatcc atggacggtg acgtcgtctt
     1741 gtagaaggtg cggtgggcgt cataggtgcc ggtgcgcagg aagtcggtca gcacgttatt
     1801 cttgttggac gcgcagacca gcttgcccac cggcaagccc atctgcttgg cgtagtagcc
     1861 ggccaggatg tcgccgaagt taccggtcgg aacgcagaag tcaaccggct tgccccatgc
     1921 gaccgcgccc cgctcggcca gacggaagta ggcgtagaag tagtagacaa tctgcggcac
     1981 caggcggccc cagttgatgg agttcgcgct ggacaggcgg atgccgcgct tttccagctc
     2041 ggcggcaacg tcggcgtcgg caaagacctt cttgacgccg gtctgggcgt cgtcaaagtt
     2101 gccctgcacg gcgtagacct gcacattttg gcccttctgg gtcgccatct gcaggtgctg
     2161 gatctcgctg gtgccgtcgt tcggatagaa cacttccatt tcaatgccgt cgagatcagc
     2221 ataacccgcc agcgcggcct tgccggtatc gccggaagtc gcaacgagaa tgcgggtcgt
     2281 ctcggtgcgg cccagcatct tcttggcttc gaccagcagc ttcggcatca gctgcaaagc
     2341 gtagtccttg aacgcgcagg tcggaccgtg ccacagttca agagaataca ggccgtcgcg
     2401 aaccttgacc gtctcgcctg ccttgccgcc gaaggcttca ccgtaggtag cagcggtggc
     2461 ggtgtgcagg aaatcagcgc tgtactcggt caggaaaccg gccagcactt tttcagccag
     2521 tacggggtac gaaaggtctt tccaaccttc cagatcagcc ttcgggaaag tttccggtac
     2581 aaacagaccg ctctgcggtg ccagaccacg gacgattgct tcctgtgcgg aaacgcggag
     2641 tgcggaatct ctggtgctta aatactgcat atgtttacac cctttctctt attctcttgt
     2701 actttcgttc ctctatatct tctcagcact caaacgcgct gggaatgcaa tgtacctgcc
     2761 agcccttggc ggcgggggtg acttccacca catcaaagcg gatggctgcg tcccggtagg
     2821 ggctgcactg caaatactgc tgggcggcta aaatcaatcg ctgctgcttg cgggcatcca
     2881 ccgcagcggc cgggcgatcc agcatcgcgc cggtacgggt cttgacctcg gcaaagacga
     2941 gggtgtcatc cttatataaa ataaggtcga tttcccccat ccgggtgcgg taattgtggt
     3001 tgagcagcag ccagccgcgc tgctggtaat acttagcggc aatcgcttcg ccgatatggc
     3061 cggtgtcagt catgccagta gccctgtttt ttcaaaaagc tgcggcggta aaacggctgg
     3121 atgccgtact gcgcaatcag ctcatagtgc agcttcgtcg ggtagccctt gtgcttggca
     3181 agctggtact gggggtattg cttgtccagt tccagcatat agcggtcacg gctgaccttt
     3241 gccagaatcg acgctgccgc aatggacgcg ctgtttgcgt cgcccttgac gacggcctcg
     3301 gcgggctgta tcagccccgg cgggcagcgg ttgccgtcaa tcagcacata atcgggcgtt
     3361 atttccagcc ctgccactgc ctgggccatg ccgtccatcg tcgcccacag gatgttgcgc
     3421 tcgtcgatct cctgcggcga gataaatacg attttgtacg ccaaggcacg gtttgtgatt
     3481 tcttcaaaca gcgcttcgcg gcgtttttcg gtcagctttt tggagtcatt gacccccgca
     3541 atggggtcgt cggggttcag gatgaccgcc gcgcagcaga ccgggccgca cagaggaccg
     3601 cgcccggcct cgtccacgcc gcagagtacg ccgtgctcgg cgcgtttggc ggcgtcaaag
     3661 gcgtagagcg cctcgttact gctcgtcgtc gtcccagtcg tccggggcat cgtcgatcac
     3721 ctcttctgct tcttcttgaa tcgcacgctg gggcgggcgc tccagcgaga gccgccccca
     3781 cttgctggcg cggaactcac ccaccagcat ttttgcggcg cgttcggtgt ccacctcgcc
     3841 gccggaaatc agcatcccgc gcttggcacc tatggcctgc agcaggtcgt agggcggcag
     3901 ttccagcgtc gccgcatcca gcttgtagcg ggccaaaagc tgctgggggt agatgccgcg
     3961 aacgctcgag agcagaccgc aggccagttc ctcaatgtcc aggatatcgt cgcggatgga
     4021 gccgatgaac gcgaggttcg acgccgtttc cagcgagtca aactttttcc acagcacgcc
     4081 gggcatatcc atcaagtcaa agttgtcaac gctgatccac tgcttgccgc gggtgacgcc
     4141 gggcttgttg gcagccttgg cgcgggtcgt gcccgcaaag gagttgataa aggtggactt
     4201 gcccacgttc gggatgccga cgatcatcac gcggatggcc gcacccacca tgccgcggtt
     4261 gcggcggcgc tccatcaggg cggagagttc tttctcaatt aaccctttcg tcgccgtggc
     4321 gcggccccgc tgcttggagt ccatggcaat gcagcccgcg cccgcgctct taaagtaggc
     4381 cagccactgc ttggtgattt cggggtcggc caggtcggat ttgttcagta catataaatg
     4441 cggtttgccc gcggtgatgc gctcgatctc ggggttcagg ctggaaagcg ggatgcgtgc
     4501 gtcgagaatc tgcaaaacgc agtcgacgtt gcggatttcc ttttccatca ggcgcaaggt
     4561 tttggtcatg tgcccgggga accactggat ctggcggctg ttgataatgt cgctctggct
     4621 cattcggccg ctcctatctt gccaaagggc agtatgcgca gcatggctgc gcccataatg
     4681 tcacgttcgt ccacgcagcc cacgcgggaa tcgcggctgt cggtggaatt gttgcggttg
     4741 tcgcccatca caaaaaggca gccgtccggc accgtgatgg gaaagtccac gctctcatat
     4801 aaataggtag gctctgcggt gtacggttcg tcgagggcga cgccgttgcg atagacgatg
     4861 cccgcggcga aatcaatgtt gatggtatcg ccgcccttgc cgatgacccg cttgaccagc
     4921 ggctttccgt aactggtgta gccgtccacc acgacgatat cgccgtagtc gggctgcttc
     4981 atcaggttga tgaggatgcg ctcgccgttg aagagcgtcg gatccatcga gtggccgtcc
     5041 accttgacga tgcgcacaaa aaaggtgaac acaatggcaa tgactgccac ggccatggcc
     5101 agggcgtcgt accattccag cgcttcctta ttgcggcggt agcgtttttt gaacgattca
     5161 tcaaacgctg cacccat
//
LOCUS       DBMDSQ010000128         5170 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_3923_length_5170_cov_1.492405, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000128 DBMDSQ010000000
VERSION     DBMDSQ010000128.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 5170)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5170)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5170
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3923_length_5170_cov_1.492405"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(366..773)
                     /locus_tag="ACZPAX_05780"
     CDS             complement(366..773)
                     /locus_tag="ACZPAX_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015565277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RrF2 family transcriptional regulator"
                     /protein_id="HIR1113440.1"
                     /translation="MKFSTRDRYALRLMVELANRPNDLIPLKEISDKQRISLKYLEQI
                     VTPLAKAGLVSSVRGAQGGYRLARSAAEITSGEILRAVEGELTAIPCLASSVDCPHRG
                     QCHTLDFWSGLNDLINKYVDGVTLHQLRNLPEE"
     gene            1263..2156
                     /locus_tag="ACZPAX_05785"
     CDS             1263..2156
                     /locus_tag="ACZPAX_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HIR1113441.1"
                     /translation="MKKLISATLAATLALSLAACGSTASTTETASSEAVSTESTTASS
                     EAAESTSDLAGTTLKVAASPTPHAEILNVAKEVLAEQGIDLEVVEFSDYVQPNLVTEN
                     GEVDANYFQHTPYLDSFNEENGTHLVSVGAVHYEPFGIYPGKSSDLANIADGATIAVP
                     NDTTNEARALQLLAAQGLITVRDGAGLTATVNDITENPHNVKIEEIEAAQLPRTVQDV
                     DFAVINGNYAIEAGFSVGKDALATEDASSEAAQTYANVLVVKEGNESDPAIQALYAAL
                     TSDKVKDYINSTYDGAVVPIF"
     gene            2325..3323
                     /locus_tag="ACZPAX_05790"
     CDS             2325..3323
                     /locus_tag="ACZPAX_05790"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HIR1113442.1"
                     /translation="MIELQHLTKRFATQGGTVVALNDINLTIRDGDVYGIIGMSGAGK
                     STLVRCINMLERPDEGSVIVNGKQMQDLNAADLRAARREITMIFQQFNLLMQRTCLRN
                     IMFPMELAKVPKEKAEARARELLELVGLPDKADAYPAQLSGGQKQRIAIARALATDPK
                     VLLCDEATSALDPNTTHAILQLIQKINRELGITVVIITHQMSVVEEVCNRVAILDNGT
                     VVEEGGVQAIFSHPTSKAARRLVYPAGAPKAEALPGQRLVRVAFGGTQTTDKPLVASL
                     AIECGALVSIMAADTRIVNGQTLGSMLLALPDNDKAEAAIEYIKNYPGITYEEVNG"
     gene            3323..4021
                     /locus_tag="ACZPAX_05795"
     CDS             3323..4021
                     /locus_tag="ACZPAX_05795"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HIR1113443.1"
                     /translation="MAEFFAGKDWQTFLQILPTIPFETWETIWSTVVATLLALVIGLP
                     LGVCLVTGEQGGVRPLPRWVMALLNGLVNLLRSVPFLILMIIVIPLSRAIVGTSIGTV
                     ASIVPLVIASFPFVSRLVETSMREVDAGVIEAAQSMGATPLQIITKVMVPESMPSLVA
                     NVTIAATTIMGYGAMAGIIGGGGLGKIAINYGYYRFNVVLEFFATALLVVLVQILQTV
                     GTKLAIKCDKRIQK"
     gene            complement(4364..>5170)
                     /locus_tag="ACZPAX_05800"
     CDS             complement(4364..>5170)
                     /locus_tag="ACZPAX_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004793 - threonine aldolase activity
                     [Evidence IEA];
                     GO_process: GO:0006567 - L-threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine aldolase family protein"
                     /protein_id="HIR1113444.1"
                     /translation="DSGHIHVHETGAVEATGHKCLALPNKNGKITARQIRHAVEEHRA
                     NASHEHEVQPGMVYISNPTEVGTLYNKEELTELSAACYELGLYLFVDGARMAYGLTSP
                     ANDLSLQDYAALCDVFYLGGTKCGALFGEAVVITNDDLKLDFRYCIKQHGGMLAKGRL
                     LGEQFLALLDGEDGGETSLYFTMARHANEQALHIRAAFEAKGCKVLHDSPTNQQFFAL
                     PDEWYAKLTERYAMTHMDKPDKHHTAVRICTSWATKDETVEQLIEDVNAL"
BASE COUNT         1120 a         1553 c         1467 g         1030 t
ORIGIN      
        1 cccagcacct acgcctgaac cgacccagga acccacacct acccccagcc ctacgcccat
       61 gccggaagtc acttctgttc cggaaagtac agacaccaac ggcaatgata ccggcggtgg
      121 tgaaactgac ggcgaagaca atgccaacac aactaacgag aatacataac agcaaaaggc
      181 aagtgcagat caattgcact tgccttttta ttgcgcattt acttttggcc ccctctgcga
      241 gggggctcct gcgaagcagg tgggggagag agcctttccg cacttcgaag ctttggataa
      301 agggcaagct ctctccctcc ggcccttcgg gccacctccc tcacagaggg aggcattggg
      361 cgcacttatt cttccggcag gttccgcagc tggtgcaggg tcacgccgtc cacatatttg
      421 ttgataagat cgttcagccc gctccagaaa tccagcgtgt ggcattggcc gcggtggggg
      481 caatccaccg aggaagccag acacggaatg gccgtcagct ccccttctac ggcgcgcaga
      541 atctcgccgg aggtgatttc cgccgccgaa cgggccaggc ggtagccgcc ctgggcaccg
      601 cgcacgctgg ataccagccc cgcctttgcc agcggggtga cgatctgctc caaatatttt
      661 aagctgatgc gctgcttgtc gctgatttcc tttaacggga tcagatcatt ggggcggttg
      721 gcaagctcca ccatcaggcg caaagcgtaa cggtcacggg tcgagaattt catgttcagt
      781 ctcccttttc ctgagattgt actactaatc atagcataat tataggaatt ttgcaagcat
      841 tcaacctatc tgcctggtag gatatatgca aaatgccgaa atgtgcaatt ttatgcaaaa
      901 gggtgttgac agaatgtgtt cactggcgta tactgtgagc catggaaagc aaatcaccgc
      961 tgaccaaaca acgagagaaa ggggtacaag actatgaacg cagcctattc cggcatgatg
     1021 atgtgcatgt gcatgatgcg taagcgtcct gtgccagctt tcttgcgcgc aaatttcagc
     1081 gcataaagaa aacttgcagc gggatgccgg agaaaaccga catcccgctt ttgtttggaa
     1141 aggaagtttg tgactatgga aagaggtccc atctacaagc agcccggtgt gcgaatgtga
     1201 cgtttgaggg aacaacacat tcacaaaaca acacacctga caataaaaag gagattacta
     1261 ccatgaaaaa actgatttct gcaacgctcg ccgctaccct ggccctgagc ctggccgcct
     1321 gcggctccac cgcttccacc accgagaccg cttcttccga ggctgtctcc accgagagca
     1381 ccacagcttc ttctgaggct gccgagtcca ccagcgacct ggctggcacg accctgaagg
     1441 tcgccgcctc ccccacgccc cacgccgaga tcctgaatgt ggccaaggag gttctggcgg
     1501 agcagggcat cgaccttgag gtcgttgagt tcagcgatta cgtccagccc aaccttgtga
     1561 ccgagaacgg cgaggttgac gctaactact tccagcacac cccctacctc gacagcttca
     1621 acgaggagaa cggcacccac ctcgtcagcg tcggcgctgt ccactatgag ccgttcggca
     1681 tctacccggg caagagcagc gacctcgcca acatcgctga cggcgcaacg atcgccgtgc
     1741 ccaacgacac caccaacgag gcccgcgccc tgcagctgct ggctgctcag ggtttgatca
     1801 ccgtgcgtga cggcgccggt ctgaccgcca ccgtcaacga catcaccgag aacccccata
     1861 acgtgaagat cgaggaaatc gaggctgccc agctaccccg caccgtgcag gacgttgact
     1921 ttgccgtcat caacggcaac tacgcaatag aggctggctt ctccgtcggc aaggatgctc
     1981 ttgccaccga ggacgcatcc tctgaggctg cccagaccta cgccaacgtt ctggtcgtga
     2041 aggaaggcaa cgagagcgac cccgccatcc aggccctgta cgccgccctg accagcgaca
     2101 aggtcaagga ttacatcaac agcacctatg acggcgcggt cgtgcccatt ttctaaggaa
     2161 aaacatatcc gcggtaaggt cgtaggggcg aacattgctc gcccctacag tgcaaggcac
     2221 caaacagcaa gaaaaacctt gcaacctgtc gtgaaataga gtagaattaa cagcgaatgg
     2281 gttttacact cattcgctat ctttgtaagt gagggagaat acccatgatc gaactgcaac
     2341 acctgaccaa gcgctttgcc acgcagggtg gcacggtcgt cgcgctgaac gacatcaacc
     2401 tgaccatccg ggacggcgat gtctacggca tcattggtat gtcgggcgcc ggaaaatcta
     2461 cgctggtacg ctgcatcaat atgttggaac gccccgatga gggcagcgtc atcgtcaacg
     2521 gaaaacagat gcaggacctg aatgcggctg acctgcgcgc cgcccgccgc gaaattacga
     2581 tgatcttcca gcagttcaac ctgctgatgc agcggacctg cctgcgcaac attatgttcc
     2641 cgatggagct tgccaaggtc cctaaggaaa aggccgaggc ccgcgcccgc gaactgctgg
     2701 agctggtcgg cctgcctgac aaggccgacg cctaccctgc ccagttgtcc ggcggccaga
     2761 aacagcgcat tgccattgcc cgcgccctgg cgaccgaccc gaaggtgctg ctctgcgacg
     2821 aagcgacgag tgcccttgac cccaacacga cccacgccat cctgcagctg atccagaaga
     2881 tcaaccgcga actgggcatc acggtcgtca tcatcaccca ccagatgagc gtggttgagg
     2941 aagtctgcaa ccgcgtggcg attttggaca acggtaccgt tgtcgaggag ggcggcgtgc
     3001 aggccatctt cagccatcct acttccaagg cggcacgccg cttggtctac cccgccggtg
     3061 cacccaaagc cgaggccctg cccggccagc gcctggtgcg tgtggccttc ggcggcaccc
     3121 agaccaccga caagccgctg gtggcaagtc ttgccatcga gtgtggtgcg ctggtatcca
     3181 tcatggcggc cgatacccgc attgtcaacg gccagacgct gggcagcatg ctgttggctc
     3241 tgcccgacaa cgacaaggcc gaagcggcca tcgagtacat taaaaattat cccggcatta
     3301 cctatgagga ggtgaacggc tgatggcaga gttttttgca ggcaaggact ggcagacgtt
     3361 cttgcagatt ttgcccacca ttccgtttga aacgtgggaa acgatttggt ccacggtcgt
     3421 ggccaccctg ctggcgctgg tcattggcct gccgctgggt gtctgccttg tcaccggcga
     3481 gcaggggggc gtgcgtcccc tgccccgttg ggtcatggcg ctgctgaacg gcctggtcaa
     3541 cctgctgcgc agcgtgccgt tcctgattct gatgatcatt gtcatcccgc tgtcccgcgc
     3601 catcgtgggc acatccatcg gtacggtggc atccattgtg ccgctggtca tcgcgtcctt
     3661 ccccttcgtc tcgcgcctgg ttgaaacgag catgcgcgag gtcgatgcgg gcgtcatcga
     3721 ggccgcccag agcatgggtg cgacgccgct gcaaattatc accaaagtca tggtgcccga
     3781 gagcatgccc agcctggttg ccaatgtgac gattgcggct acgaccatca tggggtacgg
     3841 tgccatggcc ggtatcatcg gcggcggcgg cctgggcaaa attgccatca actacggcta
     3901 ctaccgcttt aacgtggtgc tggagttctt cgccaccgcc ctgctggtcg tgctggtgca
     3961 gatcttgcag accgttggca cgaagctggc catcaagtgc gataagcgca tccagaaata
     4021 aattatatga aattgcacag taggggcggg gctctgctcc gcccgggaac gtggtctcgg
     4081 ccgcaaagtc cccgggcgga ctggaagccc gcccctactg cgttataggg aaaagcaaag
     4141 aattaacata tttttcaaat tggcgtgaca ggccagacat aaatgtggtg tagtatttag
     4201 acagagtcaa ggggatgccc cgagacaaca actaccactg acgccccggc catgccagaa
     4261 gcaatcgctt cggccgcggc gcagacaacc aataaggccg cgaagccatt tgaatagatg
     4321 aaaagtcccg catcgtgtga tgcgggattt ttggtgtact gaattacagc gcgtttacgt
     4381 cctcaatcag ctgctccacg gtttcatcct ttgtggccca gctggtgcag atgcgcacgg
     4441 cggtgtggtg cttgtcaggc ttgtccatgt gggtcatggc gtagcgctcg gtcagcttgg
     4501 cgtaccactc gtcgggcagg gcaaagaact gctggttcgt cggggagtcg tgcaaaacct
     4561 tacagccctt tgcctcaaac gcggcacgga tgtgcagggc ctgttcgttg gcatggcgtg
     4621 ccatcgtgaa gtacaggctc gtctcgccgc cgtcctcgcc gtccagcagg gccaggaact
     4681 gctcacccag cagacggccc ttggccagca tgccgccgtg ttgcttgatg cagtagcgga
     4741 aatccagctt cagatcgtcg ttggtgatga cgactgcctc gccgaacagc gcaccgcatt
     4801 ttgtgccgcc caggtagaac acgtcgcaca gggcggcgta atcctgcagg ctcaaatcgt
     4861 tggcggggct ggtcagaccg taggccatgc gggcaccatc cacaaacagg tacagcccca
     4921 gctcatagca agcggcggaa agctcggtca gctcttcctt attataaagg gtgccgacct
     4981 cggtggggtt cgagatatac accataccgg gctgcacctc gtgctcgtgg ctggcgttgg
     5041 cgcggtgttc ttccaccgcg tggcggatct gccgtgccgt gatcttgccg tttttattgg
     5101 gcagggccag gcacttgtgg ccggtggctt ctactgcgcc ggtctcgtgt acatggatat
     5161 ggccgctgtc
//
LOCUS       DBMDSQ010000129         5169 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_3924_length_5169_cov_2.031373, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000129 DBMDSQ010000000
VERSION     DBMDSQ010000129.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 5169)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5169)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5169
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3924_length_5169_cov_2.031373"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(355..1026)
                     /locus_tag="ACZPAX_05805"
     CDS             complement(355..1026)
                     /locus_tag="ACZPAX_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005926398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-8X methylmutase"
                     /protein_id="HIR1113445.1"
                     /translation="MTPQHHLPADIERTSMGIITAELAARGLQIPEESAAVVKRVIHT
                     TADFDYAENLHFTSGAVAAGMAAVRSGVTIVTDTNMALAGITKPGLARLGGQALCFMA
                     DPAVAAAAKQAGTTRAVAAMHKAARDCPGAVLAVGNAPTALLTIVEKIENGLRPALVI
                     GVPVGFVNVVESKERLFAVCTQYGVPAIVAMGRKGGSNVAAAICNALIYSAAEMLDPA
                     ARGWQ"
     gene            complement(1023..2522)
                     /locus_tag="ACZPAX_05810"
     CDS             complement(1023..2522)
                     /locus_tag="ACZPAX_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005943229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HIR1113446.1"
                     /translation="MSKAKCIMVQGTMSGAGKSLLCAALCRIFAQDGYKTAPFKSQNM
                     ALNSFVTRDGLEMGRAQVVQAQAAGVEPDVRMNPILLKPSSDIGSQVIVNGEVRGDMP
                     AQEYFRHKKALIPDILRAYDSLAEEFDVVVIEGAGSPAEINLKADDIVNMGLAKLVNA
                     PVLLAGDIDRGGVFAQLYGTVELLEPDERARIKGLIINKFRGDVEILRPGLTMLEEKT
                     RLPVVGVVPYLKVDIEDEDSLSTRLEQKSAVKPLDIAAVRLPHISNFTDFIPLEQHPL
                     LSVRYVQTARELGAPDLVILPGTKNTVGDLLWLRQSGLEAALLKLAAGGTPVLGVCGG
                     YQMLGQTLDDSQGTESGRPQTLRGLGLLPTQTIFTADKRRAQIEASAAAPFAGAALTG
                     YEIHTGRTAVQGAPFCTHADGTSEGCVQDTVFGTYLHGLFDSGELTERLAAYLCARKG
                     IAPEAAAPIPMAAYREQQFDLLADGVRRALDMDAVYAAIGLVNPKGDKK"
     gene            complement(2519..3571)
                     /gene="cobD"
                     /locus_tag="ACZPAX_05815"
     CDS             complement(2519..3571)
                     /gene="cobD"
                     /locus_tag="ACZPAX_05815"
                     /EC_number="4.1.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015538416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine-phosphate decarboxylase CobD"
                     /protein_id="HIR1113447.1"
                     /translation="MKLVHGGDWAGYRAEFGRDALDFSANVSPLGLPAGVAAAITAAL
                     PMADRYPDPLCRDLRTALAEAEGVPAEQILCGNGAADLIFRLALALRPRRALLPAPTF
                     AEYAAALDTVGCTVQRVFLQEENDFTVTDDFVDAVTDDIDAVFLCQPNNPTGQVTPPE
                     LVQKLLRRCVACGAVLVVDECFLDFLPDRDALTAKRLLHDASNLIILKAFTKLYAMAG
                     VRLGYALCGNAALMDKMRAAGQPWAVSSLAQAAGLAALGETAYADCVRDLIAEERPKL
                     AAGLRALGLYVVGGRANYLLFQAPADFGETLRRRGAVVRSCANYPGLDGSWYRTAVRT
                     NAENQKLLQIMGEVLA"
     gene            complement(3582..4544)
                     /gene="cbiB"
                     /locus_tag="ACZPAX_05820"
     CDS             complement(3582..4544)
                     /gene="cbiB"
                     /locus_tag="ACZPAX_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016321212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0048472 - threonine-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-phosphate synthase CbiB"
                     /protein_id="HIR1113448.1"
                     /translation="MIFWAVLGGFLLDAVFGDPVWLLHPVVLMGRCITALEKRLRAAL
                     PKTPQGELAGGCVVAAVLPLGTLAVTGLSCWGAAKIHPLLGLALQMFWCAQALAARDL
                     VRESMNVYRELQRGDLPAARKAVARIVGRDTQNLTAEGVTKAAVETVAENASDGVIAP
                     LLYMLIGGAPLALTYKAINTMDSMLGYKNERYLYFGRCAAKLDDAANWLPSRLAALLW
                     VCAAALTGSSAAGAWHIWRRDRCNHASPNSAQTESACAGALGVQLAGPAYYFGEYYDK
                     PTIGDPLRPVEPQDILRADRMMYAESVLALALGLAVRALLVFGL"
     gene            complement(4541..5140)
                     /locus_tag="ACZPAX_05825"
     CDS             complement(4541..5140)
                     /locus_tag="ACZPAX_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005926385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HIR1113449.1"
                     /translation="MLIYFLRHGLTDYNAQKRYQGQRDIPLSAQGLAQLRKADIDPKV
                     VYISTLQRTKQTAEVLFPHAKLVPVDALKEMCFGSFEGRNYVEMEHDPDYLAWVAANC
                     ESQCPDGERKAEFSDRICKAFAALVDKALADGEELLVILAHGGTQMAALERYALPHKD
                     YYEWCGPNAGGYVLDARDWPTQHILHLVKTVQYTKEGTC"
BASE COUNT          985 a         1703 c         1494 g          987 t
ORIGIN      
        1 ttgcaaatga agtacctgct gaaaaatggc actattttgc gatgtttact atttccaatg
       61 tagtgtttct ggccattctg atgggcgctt gggccctgat ctgacctgat atttccgtaa
      121 ttctatttta caaagtgcaa gctctttaca aacaaaaaga cccgcagcag caggatacta
      181 aagtaccatt cccgtgttca actatatctg cccgcacctt tctgcaaaat tcaggcagga
      241 agccggagaa acgctgacgg attttatagg gaaacaaaaa agcggaggag gcaaaacgcc
      301 tcctccgcgc caaaattttc ggttgtaggg aaacaccggg gggtagaaag tgtattactg
      361 ccatcctctt gccgcagggt cgagcatctc ggcggcggag tagatgagag cattgcagat
      421 ggcggcggca acgttgctgc cgcccttacg gcccatggcc acgatggcag gcacgccata
      481 ctgcgtgcag acggcaaaaa ggcgttcctt gctttccacc acgttcacaa agcccaccgg
      541 cacgccgatg accagcgccg ggcgcaggcc gttttcgatt ttttccacga tggtcagcag
      601 cgccgtgggg gcgttgccga cagccagcac cgcgccgggg cagtcccgtg cggccttgtg
      661 catggcggcc acggcgcggg tcgtgcctgc ctgcttggcc gctgcggcca cagcggggtc
      721 cgccataaag cagagcgcct gcccgcccag ccgcgccagt ccgggcttgg tgatgcctgc
      781 cagcgccata tttgtgtcgg tgacgatcgt cactccgctg cgcacggcgg ccatgcccgc
      841 cgccaccgcg ccggaggtga agtgcagatt ttcggcatag tcaaaatccg ctgtggtgtg
      901 gatgacccgc ttgacaacgg cggcgctttc ctccgggatt tgcaatcccc gcgcggccag
      961 ttccgccgtg atgatgccca tgctcgtgcg ctcaatatca gcgggcaaat gatgctgcgg
     1021 cgtcattttt tgtctccttt cgggtttaca agacctatgg ctgcgtagac agcgtccata
     1081 tccagggcgc ggcgcacacc gtcggccagc aggtcgaatt gctgttcgcg gtaggcggcc
     1141 atcggaatgg gcgcggcggc ctcgggggca atccccttgc gtgcgcagag atacgccgcc
     1201 agccgctcgg tcagctcacc gctgtcaaac aggccatgca gataggttcc gaacaccgtg
     1261 tcctgcacgc agccctcgga tgtgccgtcg gcgtgggtgc agaacggtgc tccctgcacg
     1321 gcagtacggc ctgtgtggat ttcgtacccg gtcagcgcgg cccccgcaaa gggcgcggcg
     1381 gcgctggctt cgatttgggc gcggcgcttg tcggcggtaa aaattgtctg tgtcggcagc
     1441 agtcccagcc cgcgcagcgt ttgcgggcgg ccgctctcgg tgccctggct gtcgtccagc
     1501 gtttggccca acatctggta gccgccgcag acgcccaaca ccggcgtgcc gcctgccgcc
     1561 aatttcagca gcgcggcctc cagtccggac tgccgcagcc aaagcaggtc ccccaccgtg
     1621 tttttcgtgc cgggcaaaat gactaaatca ggcgcaccca gctcccgcgc ggtctgcacg
     1681 taccgcacgc tcagcagcgg gtgctgctcc agcggtataa aatcggtgaa attcgagata
     1741 tgcggcaatc gcacagcggc gatatccaac ggttttacgg cacttttttg ttccagccgc
     1801 gtcgagagcg agtcctcgtc ctcgatatcc actttcagat acggcactac gcctaccacc
     1861 ggcaggcggg tcttttcttc cagcatcgtc agaccagggc gcaggatctc cacatccccg
     1921 cggaatttat tgatgataag tcctttgatc cgtgcccgct catcgggttc cagcagttcc
     1981 accgtaccgt acagctgcgc aaacacgccg ccccggtcga tatccccggc cagcagcacc
     2041 ggggcgttca ccagcttcgc cagccccata ttgacgatgt cgtcggcctt caggttgatc
     2101 tcggccgggc tgcccgcgcc ctcgatgaca acgacatcga attcctcagc cagactgtcg
     2161 taggcacgca gaatatctgg gatcagcgcc tttttatggc ggaaatattc ctgtgcgggc
     2221 atatcgccac gcacctcgcc gttgacgatg acctggctgc caatgtcact gctgggtttg
     2281 agcaaaatcg ggttcatccg cacatccggc tccacgcccg cggcctgggc ctgcacgacc
     2341 tgggcgcggc ccatttccag cccgtcgcgg gtcacaaagc tgttcaacgc catgttctgg
     2401 cttttgaacg gtgccgtctt gtagccatcc tgggcaaaaa tgcggcagag tgcggcacag
     2461 agcaggcttt tccctgcacc gctcatcgtg ccctgcacca tgatacattt tgctttgctc
     2521 acgccagcac ctcccccatg atttgcaaca gcttttgatt ttcggcgttg gtacgcacag
     2581 cggtacggta ccagctgcca tccaaacccg ggtagttggc gcagctgcgc acgaccgcac
     2641 cgcgccggcg cagggtttcg ccaaagtcgg caggggcctg gaacagcagg tagttggccc
     2701 ggccccctac cacgtacaat cccagggcac gcagtcccgc tgccagcttg gggcgttcct
     2761 cggcaatgag gtcccgcaca cagtcggcgt aggcagtctc gcccagcgcc gccagccctg
     2821 ccgcctgcgc aaggctggac accgcccacg gctgccccgc cgcacgcatt ttgtccatca
     2881 gcgcggcatt cccgcacagc gcgtacccca gccgcacgcc cgccatggcg tagagcttgg
     2941 taaaggcctt taggatgata agattcgatg catcgtgtaa aagtcgttta gcagtcagtg
     3001 catcgcggtc gggtaaaaag tccaaaaagc actcgtccac caccaacacc gcgccgcagg
     3061 cgacacagcg gcgcagcagt ttttgcacca gttccggcgg tgtgacctgc ccggtagggt
     3121 tattgggctg gcacaaaaag acagcgtcga tatcgtcggt cacggcatcc acaaagtcat
     3181 ccgtcacggt aaagtcgttt tcttcctgca aaaacacacg ctgcaccgta cagcccacgg
     3241 tatccagcgc agcggcatac tcggcaaagg tgggcgcggg cagcagcgca cggcgcgggc
     3301 gcagggccag cgccaggcgg aaaatcaaat ccgctgcccc gttgccgcac aaaatttgtt
     3361 cggcgggtac gccctcagcc tctgccagcg ccgtccgcag gtcgcggcac aacggatccg
     3421 ggtagcggtc tgccattggc agcgccgccg tgatggccgc cgccacgccc gcaggcagac
     3481 ccagtgggct gacattggcc gaaaaatcca gcgcatcgcg gccaaattct gcccggtagc
     3541 ccgcccagtc tccgccatgt accaacttca tttttgttgc ctcacaatcc aaacacaagc
     3601 agtgcccgca cggccagccc caaggccagc gccagcacgc tctcggcgta catcattcgg
     3661 tcagcccgca gaatatcctg cggttccacc gggcgcagtg ggtcgccgat cgtcggttta
     3721 tcgtaatact cgccaaaata gtaggctggc cctgccaact gcacgcccaa ggccccggcg
     3781 caggcgctct cggtctgggc gctgttgggg ctggcgtggt tgcagcgatc ccgccgccag
     3841 atatgccatg cccccgccgc gctgctgccg gtcaaggccg ccgcgcagac ccacaagagg
     3901 gcggccaggc ggctgggcag ccagttggca gcgtcgtcca atttggcggc acagcgccca
     3961 aaatagagat atctctcgtt tttgtagccg agcatactat ccatcgtgtt tatggccttg
     4021 taggtcagcg ccaacggtgc accaccgata agcatataca aaagcggcgc gatcacaccg
     4081 tcgctggcgt tctcggccac tgtctctacg gcggctttcg tcacgccctc ggcagtcagg
     4141 ttctgggtgt cccgccccac aatacgcgcc acggccttgc gggcggcggg caggtcgccc
     4201 ctttgcagtt ccctgtacac gttcatgctc tcccgcacaa gatcacgcgc cgccagcgcc
     4261 tgggcgcacc agaacatttg cagcgccagc cccagcagcg gatggatttt tgctgcaccc
     4321 cagcaggaaa gcccggtcac agccagtgtc cccagcggca gcaccgccgc cacaacgcag
     4381 ccgcccgcca gctcaccctg cggcgttttg ggcagggcgg cacgcaggcg cttttccagc
     4441 gccgtgatgc accgccccat cagcacgaca gggtgcagca gccacaccgg atcgccgaaa
     4501 acggcatcca gcaaaaaacc gcccagcacc gcccaaaaaa tcaacacgtg ccctccttgg
     4561 tgtactgcac ggtcttgacc aggtgcaaaa tatgctgcgt cggccagtcg cgggcgtcca
     4621 gcacatagcc gcctgcattg gggccgcacc actcataata gtccttgtgc ggcagggcgt
     4681 aacgttccag cgcggccatc tgggtgccgc cgtgggccag gatgaccagc agttcctcgc
     4741 cgtcggccag agccttgtcc accagcgcgg caaacgcctt gcagatgcgg tcggaaaatt
     4801 cggccttgcg ctcgccgtcg gggcattggc tttcgcagtt ggcggcgacc caagccaggt
     4861 aatcagggtc gtgctccatc tcgacataat tgcgcccctc aaagctgccg aaacacatct
     4921 ctttcagggc gtccaccggc acgagctttg catgcgggaa cagcacctcc gccgtctgtt
     4981 ttgtgcgctg taaggtagaa atatagacga cctttgggtc gatatccgct ttgcgcagct
     5041 gggccagccc ttgcgccgag agcgggatat cccgctggcc ctggtaacgc ttctgggcat
     5101 tatagtccgt taaaccgtgc cgtaaaaagt aaatcagcat tcgggaagtt ctcctttcag
     5161 aaccgtggg
//
LOCUS       DBMDSQ010000130         5155 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_3931_length_5155_cov_1.943411, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000130 DBMDSQ010000000
VERSION     DBMDSQ010000130.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 5155)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5155)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5155
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3931_length_5155_cov_1.943411"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..592
                     /locus_tag="ACZPAX_05830"
     CDS             <1..592
                     /locus_tag="ACZPAX_05830"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HIR1113450.1"
                     /translation="SPYSASKASADMFVKAFHDTYGMPMNITRCSNNYGPYQFPEKLI
                     PLLINNAKQHKNLPVYGDGMQIRDWLYVMDHCKAIDMVAQGGKDGEVYNVGGHNERPN
                     IFIVKTVIAQLHDRLKDEGISEELITHVADRLGHDRRYGIDPTKIKEDLGWYPETPFE
                     KGIVLTIDWYLAHPDWMAHVTSGDYQKYYEQMYKNK"
     gene            716..1618
                     /locus_tag="ACZPAX_05835"
     CDS             716..1618
                     /locus_tag="ACZPAX_05835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113451.1"
                     /translation="MANYREEHIRNNRRLARQIVGGAALLLALIGLFTVLGWVIGLVR
                     SALDDSSLRDEYHDRLYGMVMLDPVAFDDVSTVDEGVFKQAAIWGTVYQVQNSGGSLD
                     QYERDPDTGSALIPALEIDTYISNLLGPDYQVTEGSFSTSEFVYQYDEEKQAYYVPVT
                     SSVALYTPTVEMISQKDGERIVTVGYVPTATNNTTGELSLMAPTEPTKYMDYVFTRGE
                     NRQWYLTALRESDMKVEATPTPAPTDALVEDLQNDQLNTDTAPAEEAPQPEGEQPAEE
                     GDAQPAEDTAESTEDTADSESTAE"
     gene            1749..2228
                     /locus_tag="ACZPAX_05840"
     CDS             1749..2228
                     /locus_tag="ACZPAX_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005935606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HIR1113452.1"
                     /translation="MEELLRILEKNARMPIEDIAAMLDKTPEEVAAMMDEAAAKGYIR
                     GYETLVDWEQAGVNLVEAVIELRVTPHKARGFDDIGMTIAQFDEVDTVLLMSGSYDLQ
                     VIIKGRSFQEIAIFVAKRLSPLDDVLSTATSFVLRPYKRGGRLYLAEEPDERECTVL"
     gene            2228..3400
                     /locus_tag="ACZPAX_05845"
     CDS             2228..3400
                     /locus_tag="ACZPAX_05845"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HIR1113453.1"
                     /translation="MNYDELLAPAAKAMRPSGIRKFFDLAADMPHCISLGVGEPDFKT
                     PWSVRDAGIRSLELGRTKYTANAGLKDLRKEICTYLQRRFELHYQEENVLVTVGGSEA
                     IDLAIRALVQPGDEVIIPEPCFVCYEPITQLIGGVPVHIATRAEDQFRLTAEQLRAAI
                     TPKTKLLIFPYPNNPTGAVMSAAEVEEIAAVLRETNVLVLSDEIYSELTYGLDRHVSI
                     ASLPGMAERTIVVNGFSKSYAMTGWRLGYAVGPAPLIKVMTKIHQSCIMSAPTTSQYA
                     AITALRQCDDQIEMMRDEYNRRRRYVVKALNEMGLTCFEPRGAFYVFPSIQISGLTSS
                     EFCEQLLREKEVAIIPGSAFGASGEGYARISYAYSVDHLQTAMKRIREFLKEHGWI"
     gene            3429..4325
                     /gene="ispE"
                     /locus_tag="ACZPAX_05850"
     CDS             3429..4325
                     /gene="ispE"
                     /locus_tag="ACZPAX_05850"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HIR1113454.1"
                     /translation="MQYRAVTVLAPAKLNLSLDVVGTLPNGYHDLDMVMQTIDLYEKI
                     TLRRSNDLTLTLPGSFVPANDKNTAVKAALAFFHYTGLLAGVDMTIYKRVPVRAGMAG
                     GSADAAGVLVGLNELYNARLSMSELCAIGAGIGADVPFALLGGTCRVRGVGDLMKALP
                     PCPDCWFVVAMPSVGVSTPEAFARYDTMGSPVHPDCEAQEQAIRANDLRGVCAAAGNA
                     LEHCSGAVETPAICKTLRAHGAVTAQMTGSGAAVFGIFDDENAARNAAVELKKSYKQV
                     YVCKPTHGGPRVTVRRAFGAKK"
     gene            4378..5001
                     /locus_tag="ACZPAX_05855"
     CDS             4378..5001
                     /locus_tag="ACZPAX_05855"
                     /inference="COORDINATES: protein motif:HMM:NF021093.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein R"
                     /protein_id="HIR1113455.1"
                     /translation="MTKHTRRVLELGIGLALALAIVLTVSADWHQRTAAQVRADTLRL
                     HILANSDTLDDQLLKLQVRDAVLAALPPAVTEAATPDEAAAALQYALPALQQAADTAL
                     HRAHSPQTARLRLEQWDFAARDYGSFTLPGGAYTALRIELGEARGRNWFCVLYPALCV
                     SGSIAAYPTADENVLVFGRFEVRCAVADFIGRLASPAGEAVSGLPAD"
BASE COUNT         1048 a         1637 c         1550 g          920 t
ORIGIN      
        1 cagcccgtac tctgcctcca aggccagcgc ggatatgttc gtcaaggcgt tccacgatac
       61 ctacggtatg ccgatgaata ttacccgctg ctccaacaac tacggcccct accagttccc
      121 cgagaagctg atcccgctgc tcatcaacaa cgccaagcag cacaagaacc tgcccgtcta
      181 cggcgacggt atgcagatcc gcgactggct gtacgtcatg gatcactgca aggccattga
      241 tatggttgcc caggggggta aggacggcga ggtctacaac gtcggcggcc acaacgagcg
      301 cccgaacatc ttcatcgtca agacggtcat tgcccagctg catgaccgcc tgaaggatga
      361 gggcatcagc gaggaactga tcacccacgt tgccgaccgc ctgggccatg accgccgcta
      421 cggcatcgac ccgaccaaga tcaaggagga tctgggctgg tacccggaga ctccgtttga
      481 gaagggcatt gtgctgacca tcgactggta tctggcccac cccgactgga tggcccacgt
      541 caccagcggc gattaccaga aatattacga gcagatgtat aagaataagt aagaagccca
      601 cctgcggcgg cagggggcat gccgccctac gatccctttc aataggcgcg tagggcgggg
      661 tgccctcgcc ccgccgcacc gcttttcaat ctcccaaagg agcaacacat ccacaatggc
      721 aaattacaga gaagaacata tccgcaataa ccgccgcctg gcgcggcaga tcgtgggcgg
      781 cgcggcgctg ctgctggcgc tcatcggtct gtttaccgtg ctgggctggg tcatcgggct
      841 ggtgcgctcg gcactggatg acagcagcct gcgcgacgag taccacgacc gcctgtacgg
      901 tatggtcatg ctggacccgg tcgccttcga cgatgtcagc accgtcgatg agggcgtgtt
      961 caagcaggcc gccatctggg gcaccgtcta ccaggtgcag aacagcggcg gcagcctgga
     1021 ccagtacgag cgcgaccccg acaccggctc ggcgctgatc cctgcgctgg aaatcgacac
     1081 ctatatctcc aacctgctgg ggccggacta ccaggtgacg gaaggctcgt tcagcacatc
     1141 ggagttcgtc taccagtacg atgaggaaaa gcaggcctat tacgtgcctg tcaccagttc
     1201 ggtggctctg tacacgccca cggtggagat gatttcccag aaggatggcg aacgcatcgt
     1261 cacggtcggt tatgtgccca ccgccacgaa caacaccacc ggtgagttga gcctgatggc
     1321 ccccaccgaa ccgacgaagt atatggacta cgtgttcacc cgcggcgaga accgccagtg
     1381 gtacctgacc gccctgcgcg agagcgacat gaaggtggaa gctaccccca cgcctgcccc
     1441 gacagacgcc ctggtggagg acctgcagaa cgaccagctg aacaccgaca ccgccccggc
     1501 ggaggaagca ccgcagccgg aaggcgagca gcccgcagaa gagggcgacg cccagcctgc
     1561 agaggacacc gcagaatcca cggaggacac cgccgacagc gaaagcacgg cggagtaaac
     1621 agtttctata tcagcatcgg catctctgta tgggggtgct gatttttttt gcaatcctat
     1681 aaacagggat gcaatctccc gacaaatatg ctataatagc cacatatatc tgagtgaggt
     1741 gtggtactat ggaagaatta ctgcgtattc tggaaaagaa cgcccgtatg cccattgagg
     1801 atatcgcggc tatgctggat aagacccccg aggaagtcgc cgccatgatg gacgaggccg
     1861 ccgccaaggg ctatatccgc ggctatgaga ctctcgtcga ctgggagcag gccggcgtca
     1921 acctcgtgga ggctgtcatt gagctgcgcg tcacccccca taaggcccgc ggctttgacg
     1981 atatcggcat gaccatcgcc cagtttgacg aggtcgatac cgtgctgctg atgagcggca
     2041 gctacgactt gcaggtcatc atcaagggcc gcagcttcca ggaaatcgct atctttgtgg
     2101 caaagcgcct gtccccgctg gacgatgtcc tctccaccgc taccagcttt gtgctgcgcc
     2161 cctacaagcg cggcggccgc ctgtacctgg ccgaagaacc cgatgaaaga gagtgtaccg
     2221 tcctgtgatg aactacgatg aactgcttgc ccctgccgcc aaggccatgc gcccctccgg
     2281 catccgcaag ttctttgatc tggcggccga tatgccccac tgcatctcgc tgggcgtcgg
     2341 cgagccggat tttaaaacgc cgtggagcgt ccgcgacgca ggcatccgca gcctggaact
     2401 gggccgtacc aagtacacgg ccaacgcggg cttgaaagac ctgcgcaagg aaatctgcac
     2461 ctacttacag cgccggtttg agctgcatta tcaagaagaa aacgtcctcg tcacggtggg
     2521 cggcagcgag gccatcgacc ttgccatccg cgcactcgtg cagcccgggg acgaggtcat
     2581 catccccgaa ccctgctttg tctgctatga gcccatcacc cagctgatcg gcggcgtgcc
     2641 ggtgcatatc gccacccggg ccgaggatca gttccgcctg acggcggaac agctgcgtgc
     2701 ggccatcacg ccgaaaacga agctgctcat cttcccatac cccaacaacc ccaccggcgc
     2761 ggtcatgagc gccgccgagg tggaggaaat tgccgccgtc ctgcgggaaa cgaacgtgct
     2821 ggtgctgtcg gatgaaatct actccgagct gacctacggc ctcgaccgcc atgtgtccat
     2881 tgcgtcgctg cccggtatgg ccgagcgcac catcgtcgtc aacggcttct ccaagtccta
     2941 cgccatgacc ggctggcgct tggggtacgc tgtcggccct gcgccgctga tcaaagtcat
     3001 gacgaaaatt caccagagct gcattatgtc ggcccccacc accagccagt acgccgccat
     3061 cacggcgctg cgccagtgcg acgaccagat cgagatgatg cgcgatgagt acaaccgccg
     3121 ccgccgttac gtcgtcaagg cgctgaacga gatgggcctg acctgttttg agccgcgcgg
     3181 cgcgttctac gtgttcccct ccatccaaat cagcggcctg acgagcagtg aattctgcga
     3241 gcagctgctg cgggagaagg aagtcgccat cattcccggc tcggccttcg gtgcgtcggg
     3301 cgagggttac gcccgcatca gctatgcgta cagcgtagac catttgcaga ctgccatgaa
     3361 gcgcatccgt gaatttttaa aggaacacgg ctggatatag aaataaaaca acaggagagt
     3421 gaccaacagt gcaataccgc gcggtaacgg tgctggcccc ggccaagctg aacctgtcgc
     3481 tggacgttgt gggcacgctg cccaacggct accacgatct ggacatggtc atgcagacca
     3541 tcgacctgta cgaaaaaata acgctgcgcc gcagcaatga cctgacgctg accctgcccg
     3601 gcagctttgt gcccgccaac gataaaaaca ccgccgtcaa ggcggcgctg gcgtttttcc
     3661 actacaccgg cctgctggcg ggcgtggata tgacgattta caagcgggtg ccggtgcgtg
     3721 ccggcatggc gggcggcagt gctgacgctg cgggcgtgct ggttggtttg aacgaactgt
     3781 acaacgcccg gctttccatg agcgaactgt gcgccatcgg cgcgggcatc ggcgcggatg
     3841 tgccgtttgc gctgctgggc ggtacctgcc gcgtgcgcgg cgtgggcgat ttgatgaaag
     3901 ccctgccgcc ctgcccggac tgctggttcg tcgtggcgat gcccagcgtc ggagtctcga
     3961 cgcccgaggc ctttgcccgg tatgacacga tgggcagccc ggtccacccc gactgcgagg
     4021 cccaggaaca ggccatccgc gccaacgacc tgcgaggcgt ctgtgcggcg gcgggcaatg
     4081 cgctggaaca ctgctccggc gcggtggaaa cgcccgccat ctgtaaaacg ctgcgggcgc
     4141 atggggccgt cacggcccag atgaccggca gcggcgcggc ggtgtttggc atttttgacg
     4201 acgagaacgc cgcccgcaat gccgccgtcg agctgaaaaa gagttacaag caggtctatg
     4261 tctgcaagcc cacccatgga ggcccccgcg tgacggtccg ccgcgcgttc ggtgccaaga
     4321 aataagttta caagctcccc tttctgccca tactccggtt gaaaggggag aactgttatg
     4381 acaaagcata cccgccgcgt gttggaactg ggcataggcc tggcgttggc actggccatt
     4441 gtacttactg tcagcgccga ttggcaccag cgcaccgccg cccaggtacg ggcggacacc
     4501 ctgcggttgc atattctggc caacagcgat acgttggacg accagctgtt gaaattgcag
     4561 gtgcgggacg ccgttttggc tgccctgccg cccgccgtga ccgaagccgc aaccccggac
     4621 gaggctgctg cggccctgca atacgcactg cccgccttgc agcaggccgc cgacacggcc
     4681 ctgcaccgtg cccacagccc ccagaccgca cgcctgcggt tggaacagtg ggactttgcc
     4741 gcgcgggact atggcagctt caccttgccg ggcggggctt acacagccct gcgcattgag
     4801 ttgggcgaag cccggggccg caactggttc tgcgttttgt atccggctct ttgcgtctcc
     4861 ggcagcatcg ccgcctaccc caccgccgat gaaaacgtgc tggtgtttgg aaggtttgag
     4921 gtaaggtgtg cagttgcgga ctttataggc agattggctt cccccgcggg ggaagctgtc
     4981 agcggcttgc ccgctgactg atgaggggcg gccttgccgc tgccgcccgc aaatgggccg
     5041 ctgcggcata tgcgcccctc atccggccct tcgggccacc ttctcccaag ggagaaggct
     5101 attagaaagg acaccgccat gctaaacttc atcctcggtc cctccggttc gggca
//
LOCUS       DBMDSQ010000131         5135 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_3945_length_5135_cov_1.932261, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000131 DBMDSQ010000000
VERSION     DBMDSQ010000131.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 5135)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5135)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5135
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3945_length_5135_cov_1.932261"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(356..1831)
                     /locus_tag="ACZPAX_05860"
     CDS             complement(356..1831)
                     /locus_tag="ACZPAX_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016323887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HIR1113456.1"
                     /translation="MNYKMVGFVLGRIFLIEAVLMLFPMGCAALYGEWNIILAFLWPA
                     LILLALGLVTSLRTPKNTKIYARDGLAIVALVWVLMSVFGALPFVISGEIPSFVDALF
                     ETVSGFTTTGSTILTDVEALSHGTLFWRSFSHWIGGMGVLVFAMAVLPMTDGRAMHLM
                     RAEVPGPTCGKISSKLSDSAKILYAIYLVLTVIEVIMLCAGGMPLFDSLIHAFGTAGT
                     GGFSNKALSVGAYNSPYFEVVIGVFMLLFGINFNLYYFLLLRHFKEAFASEEMHVYLG
                     IVAFSTLTITANIASMYGSVGTALRTAFFQVASIVTTTGYATADFNLWPTYSRVVIVI
                     LMFVGACAGSTAGGLKVSRVIILFKAAKQDLNKMLHSHAITTVRFEGKPLDEKTLRGV
                     HNYFNIYMLMLTLSVLFVSLDGFDIVTTFTSVATCLNNVGPGLEMVGPMGSFADFSAP
                     VKLLLAFDMLAGRLELYPMLALFAPRLWRKRINAMHEARAK"
     gene            complement(1842..3209)
                     /gene="trkA"
                     /locus_tag="ACZPAX_05865"
     CDS             complement(1842..3209)
                     /gene="trkA"
                     /locus_tag="ACZPAX_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014116560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HIR1113457.1"
                     /translation="MRIVVVGLGKVGRALTAQLTAENNDIVVIDQNPELIEDIVNIYD
                     VRGVPGNGGCYEVQKEAFEDGADLLIATTSSDEINILACLVAKKLGTQHTIARIRNPE
                     YEKQLHFMRDELGLSMVVNPEKATAREIARVLRFPTAIKREQFCRQRFELIEYRLADD
                     NPLVGLQLADLYRNIRVKILICAVARGNETIIPTGSTTLQAGDKIYLTASARDLESFF
                     RKLNLFKAKANNVMIVGASRMTYYLVKELQDIQKRVTVIDSNAARCQEMSEKFPGVLV
                     IHGDGADAELLSEERITDMDAFVALTGLDETNIILAMYASQMNSCKVVAKINRNSFAE
                     LATAKGMVDSVVSTASVTSEAIAMYVRAMQNSFESENIKTLHSLVGGRVEALEFNVAP
                     GLPFIGVPLKDLILRKGLLVAGIVRRNGQPVIPSGNDALQEGDDVVIVTTDTTLHSLR
                     DIVVK"
     gene            3474..3758
                     /gene="rpsF"
                     /locus_tag="ACZPAX_05870"
     CDS             3474..3758
                     /gene="rpsF"
                     /locus_tag="ACZPAX_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005923879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HIR1113458.1"
                     /translation="MAKYETMMVTTANLDEEASNALINKFKNLIAANGTIDSVDDWGK
                     KRLAYPINDETEGVYTVISFTSEPDFPAELDRVFKITEGVLRSMTLAVEE"
     gene            3772..4251
                     /locus_tag="ACZPAX_05875"
     CDS             3772..4251
                     /locus_tag="ACZPAX_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005923877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HIR1113459.1"
                     /translation="MLNVVAIMGRLARDPELRQTTTGKSVASFRIACDRGRRDANGQS
                     QADWIDVVAWDRQAEFVCKYFQKGSLIVVDGRLQTRTYQDKNTGANRTAVEVVANNIN
                     FAGPKSSSPASNGDAGYGAPMAAPAPRPAVQAAPAPSYSAGSNDDFALIEDEGDLPF"
     gene            4289..4531
                     /gene="rpsR"
                     /locus_tag="ACZPAX_05880"
     CDS             4289..4531
                     /gene="rpsR"
                     /locus_tag="ACZPAX_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009984529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HIR1113460.1"
                     /translation="MAMDRSSRPMHRGRKKVCSFCVDRVEHIDYKDLPRLRKYVSERA
                     KIIPRRVTGTCAFHQRELTVAIKRARYMALMPYVSD"
     gene            complement(4774..>5135)
                     /locus_tag="ACZPAX_05885"
     CDS             complement(4774..>5135)
                     /locus_tag="ACZPAX_05885"
                     /inference="COORDINATES: protein motif:HMM:NF015734.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HIR1113461.1"
                     /translation="VISQDPPAGTEKLMDDQIIQITVSSGPNLVEMPDIIGFTQANAS
                     KKLDALGIQYKMVMVDNDGTMAAGCVAKTDYLAGTKINTENVTVIVSIAGERDDTLVA
                     QTGGAESTPEPTPQPEE"
BASE COUNT         1117 a         1464 c         1451 g         1103 t
ORIGIN      
        1 gttaatatga aaaatggaga actatataaa ttcattattt tcaataaatc acgctttgca
       61 aaatggtttc aagagtattc tcgataaata caaagtgtca aattccagtt tgtgggggta
      121 ctcacatttt ttcccgccta tagcccccct tttaaaaaca gatggcaccg caaggcagta
      181 tgccccttgc ggcggccaga gggctggccg ccctacatcc tatccccgaa acgtccatac
      241 ataaaccaaa accgggcagc tgccgtaaag atggcagctg cccggttcta tgattgtatt
      301 ggtttggaat ttctttggtt ctttctttgt aaagaaagaa cacccgccct cccccttact
      361 tcgctcttgc ttcgtgcatc gcgttgatgc gcttgcgcca cagacgcggg gcgaaaaggg
      421 ccagcatcgg gtagagctcc aggcggcccg ccagcatatc aaaggccagc agcaacttga
      481 ccggggccga gaaatccgca aagctgccca tcgggccgac catttccaga cccgggccga
      541 cgttgttcag gcaggtcgca accgaggtaa acgtcgtcac aatgtcaaat ccatccagcg
      601 agacaaacag caccgacagc gtcagcatca gcatataaat gttgaagtaa ttgtgtacgc
      661 cgcgcagcgt tttctcgtcc aggggcttgc cctcaaagcg cacggtggtg atggcgtggc
      721 tgtgcagcat cttgttcagg tcctgcttgg cagccttgaa aaggatgata acgcgcgaaa
      781 ctttcaggcc gcccgccgtc gaacccgcgc acgcacccac aaacatcaga atcacgatga
      841 cgacgcggga gtaggtcggc cagaggttga agtctgccgt ggcgtagccg gtggtcgtga
      901 cgatggacgc cacttggaag aacgccgtgc gcagcgccgt accgacactg ccatacatac
      961 tggcaatgtt ggccgtgatg gtcagtgtcg agaacgccac gatacccaag tagacgtgca
     1021 tctcctcgga cgcaaatgcc tccttaaaat gacgcagcag caggaaataa tagaggttga
     1081 agttgatgcc gaacagcagc atgaatacgc cgatgacgac ctcaaaatag gggctgttgt
     1141 acgcgccgac gctcagtgcc ttgttgctga atccgcctgt gcccgccgtg ccgaaggcgt
     1201 ggatgagtga gtcaaacagc ggcataccgc ccgcgcagag cataattacc tcaatgacgg
     1261 tcagcaccag atagatggca tacagaatct tcgcactgtc gctcaatttg ctggaaattt
     1321 tcccgcaggt cgggccgggg acctcggcgc gcatcaggtg catggcgcgg ccgtcggtca
     1381 tgggcagcac agccatagcg aacaccagca cgcccatacc gccgatccag tgggaaaagc
     1441 tgcgccagaa cagtgtgcca tggctcaggg cctcgacgtc ggtcagaatc gtagagccgg
     1501 tcgtcgtgaa gccggagact gtctcaaaga gcgcatccac aaaggacggt atctcgccgg
     1561 aaatcacaaa gggcagtgcg ccaaaaaccg acatcagcac ccagaccaac gccacgatag
     1621 caaggccgtc gcgggcgtaa atttttgtgt ttttgggcgt gcgcaggctc gtcaccaagc
     1681 ccagtgccag caaaatcagc gcgggccaca ggaacgcgag gatgatattc cactcgccat
     1741 agagggcggc acaccccatg ggaaagagca tgagcaccgc ttcaattaag aagatacgcc
     1801 ccagcacaaa ccctaccatt ttatagttca tggctgtgcc ctcactttac cacaatatcg
     1861 cgcagcgagt gcagcgtcgt gtcggtggtg acgatgacca cgtcgtcgcc ctcctgaagg
     1921 gcgtcgttgc cggagggaat gaccggctgg ccgttgcggc gcacgatgcc cgccaccagc
     1981 aggcccttgc gcaggatcaa atcttttaac ggtacaccga tgaaaggcag gcccggcgca
     2041 acgttaaatt ccagcgcctc gacccggccg cccaccaggc tgtgcagcgt cttgatgttc
     2101 tcggactcaa agctgttctg catggcgcgg acgtacatag cgatggcctc cgacgtgacg
     2161 gacgccgtgg aaacgacgct gtccaccatg cccttggcgg tcgccagctc ggcaaaggag
     2221 ttgcggttga tcttggccac aaccttgcag gagttcatct gggaggcgta catggccagg
     2281 atgatattcg tctcgtccaa gccggtcagc gccacaaagg cgtccatgtc ggtgatgcgt
     2341 tcctcgctca gcagctcggc gtcggcaccg tcgccgtgga tgaccagcac accggggaat
     2401 ttttcgctca tctcctggca gcgagcggcg ttgctgtcaa taacggtcac gcgcttctgg
     2461 atgtcctgca attccttgac gaggtagtag gtcatccggc ttgcgccgac gatcatcacg
     2521 ttgttggcct ttgctttgaa gaggttcagc ttgcggaaga aggattccag atcgcgggcc
     2581 gaggccgtca ggtaaatctt atcccccgcc tgcaaggtgg tcgagccggt ggggatgatg
     2641 gtctcgttgc cgcgggcgac agcgcagatc agaattttta cacggatgtt gcggtagaga
     2701 tcggccagct gcaggcccac cagcgggttg tcgtcggcca ggcggtactc aatcaactcg
     2761 aaccgctggc ggcagaactg ctcgcgcttg atggccgtgg gaaagcgcag cacgcgggcg
     2821 atttcccgcg cggtggcctt ctccgggttg acgaccatcg acaggcccag ttcgtcgcgc
     2881 ataaagtgca gctgcttttc gtactccggg ttgcggatac gtgcgatggt gtgctgggtg
     2941 cccagttttt tggcgaccag gcaggccaga atgttgatct cgtcgctgga cgtggtggcg
     3001 ataagcaggt ctgcgccgtc ctcgaaggct tccttctgga cctcatagca gccgccgttg
     3061 ccgggcactc cgcgcacatc gtagatgttt acgatatcct cgatcagttc aggattctgg
     3121 tcgatgacaa cgatatcgtt gttttccgcc gtaagctggg cggtcagggc acggccgact
     3181 ttgccgaggc cgacgaccac gattctcata cgaacgctcc atttctttta ttgcaagggt
     3241 agtgcattat aaagcctatt atagcaaagc aggcggaaaa agcaatagga cattttcacg
     3301 gattgtacaa ggttttttaa aaaaaatcgc aattttgtac cgcacagggc agattttgcg
     3361 ttgacaaacg gcgatttacc tgctataatt agatgctgtg tgaaatcaca cggttatcct
     3421 tgtcttttcc gttaaaagag aaggcctaat gtccaaaagg aggtgcacag aatatggcta
     3481 agtacgaaac catgatggtt accaccgcga acctcgacga agaggcttcc aacgctctga
     3541 tcaacaagtt caagaacctg atcgcagcga atggtacgat cgattctgtt gatgattggg
     3601 gcaagaagcg tcttgcttac cccatcaacg atgagaccga gggtgtgtac acggtgatca
     3661 gcttcaccag cgagcccgat ttcccggctg agctggatcg cgttttcaag atcactgagg
     3721 gcgttcttcg cagcatgact cttgctgttg aggagtaagg agtaaaccga aatgctgaat
     3781 gttgttgcaa tcatgggccg ccttgcgcgt gacccggagc ttcgccagac tacgaccggc
     3841 aaaagcgttg cctcgttccg tatcgcttgc gaccgcggac gccgcgatgc caacggccag
     3901 agtcaggcgg actggatcga cgttgtcgca tgggatcgcc aggcagagtt tgtctgcaag
     3961 tatttccaga agggttcttt gatcgtggtc gatggtcgtt tgcagacccg cacctatcag
     4021 gataagaaca ccggcgccaa tcgcacggct gttgaggtcg tggcaaacaa catcaacttt
     4081 gccggtccga aatcctcgag ccccgcatcc aacggtgacg ccggttacgg cgcccccatg
     4141 gcagcccccg caccccgccc cgccgtgcag gccgcacctg cgccgagcta ttctgccggc
     4201 agcaatgacg actttgcact gatcgaggat gagggcgacc tgccgttcta acaatcgaaa
     4261 gtatcattct aacaaggagg tttccacaat ggctatggat cgttcttctc gtccgatgca
     4321 tcgtggccgc aagaaggttt gctccttctg cgttgaccgc gtcgagcaca tcgattacaa
     4381 ggatctgccc cgcctgcgca agtacgtgag cgagcgcgct aagatcattc cccgccgtgt
     4441 gaccggtacc tgcgctttcc atcagcgtga gctgacggtt gccatcaagc gtgcccgcta
     4501 catggctctg atgccctacg tcagcgacta attcgccgcg tcggcattgc caaaatgcac
     4561 tgaaaaagag cagcctcgtg aggaggctgc tcttttttgt gtttgtgtat aggcaaacgg
     4621 gcaaagttcc tccggtcatc gcttcgctcg accacctccc cactgcgtga ggaggctttt
     4681 tgcggccaaa ggccgccaga aaggatcctc gctatgcggg gagctggcca agccggcgag
     4741 gcctgagggg cttttcccgc ttttcgttat tactcactct tccggctgcg gtgtcggttc
     4801 gggtgtgctc tctgcaccgc cggtctgcgc caccagcgtg tcatcccgct caccggcgat
     4861 gctgacaatg accgtgacat tctcggtgtt gatcttcgtc ccggccaggt aatccgtctt
     4921 ggccacacac ccggccgcca tcgtgccgtc gttgtccacc atgaccatct tgtactggat
     4981 gcccagggcg tccagctttt tgctggcgtt agcctgggtg aagccgatga tgtccggcat
     5041 ctccactaag ttcgggccgc tgctgaccgt gatttgaatg atctggtcgt ccatcagctt
     5101 ttccgtgccc gcgggcggat cctggctgat gacca
//
LOCUS       DBMDSQ010000132         5124 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_3949_length_5124_cov_1.698387, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000132 DBMDSQ010000000
VERSION     DBMDSQ010000132.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 5124)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5124)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5124
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3949_length_5124_cov_1.698387"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..446)
                     /locus_tag="ACZPAX_05890"
     CDS             complement(<1..446)
                     /locus_tag="ACZPAX_05890"
                     /inference="COORDINATES: protein motif:HMM:NF013663.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HIR1113462.1"
                     /translation="MSVLLIPNPTKDAGLAVTRQAAAVLAGLGVQVLMPQTFAGEGRL
                     AGVQFLPEADAYRAADQVVTIGGDGTLLRAGLSCMRHGKPVLGVNLGRMGFLATCEVA
                     ELPTKLQRLAAEEFTLVNRGLLHAEVPAHGWRADAINDIVLFGQTRL"
     gene            complement(465..1286)
                     /locus_tag="ACZPAX_05895"
     CDS             complement(465..1286)
                     /locus_tag="ACZPAX_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HIR1113463.1"
                     /translation="MSKIRLDQYLCQNGLVQSRERAKALIMSGIVFVNEQKVDKAGEM
                     IAPDAKVEVRGHDIGYVSRGGLKLEKAMQVFPMRPDGKVCMDIGASTGGFTDCMLQNG
                     AVKVYAVDVGYGQLAWSLRTDERVINMERTNIRSVKPEDLSEPVAFFSVDVSFISLKH
                     IFPVADAICTPDAVGVCLVKPQFEAGREKVGKKGVVRDPATHREVLQMAEGYAMANHF
                     TPAGLDFSPIKGPEGNIEYLMYVQHCDTPLPLPEGLIEKVVAASHETLDKAPNLH"
     gene            complement(1283..3157)
                     /gene="dxs"
                     /locus_tag="ACZPAX_05900"
     CDS             complement(1283..3157)
                     /gene="dxs"
                     /locus_tag="ACZPAX_05900"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HIR1113464.1"
                     /translation="MSYPLLERINAPGEIKNLPMTDMPALCEEIRQFLIKSVSETGGH
                     LSSNLGVVELTVALHRVLDFPQDKLLFDVGHQCYTHKLLTGRRAGFAQLRRKDGISGF
                     PSPKESDCDAFIAGHGSAALSTAIGIARAKKLKNEPGKVVVIVGDGAFTGGMVYEGMN
                     NVSKLNNLIVILNDNKMSISKNVGQMANYLTKLRTDPKYSHVKAHMETTLERIPVAGN
                     AMVKVLQSGKQLIRRGIYKSTMFEEMGFQYIGPIDGHDVDMLTHTLTNLREQYAPVFL
                     HIVTQKGKGLKPAEENPGEFHAVSSIDLSHLTDPELSPKDSFSSTFGCALADLGDDEP
                     RVCGITAAMKYGTGLNFFKHRHPGRFFDVGMAEEHAVSFAAGLASQGLLPVVAIYSTF
                     FQRAYDQIIHDVSLMQLDVLFAVDRAGLVPGDGATHQGIYDVAFFSNVNIPVFAPSNY
                     AELRYWLTYLVREARGPRAIRYARGEEKPALAALPCTGARYDVLEPVEGAKVALVSYG
                     AETEEIIAAKDLLAQNGVQTDCVKLTQIFPLPDGLCDTLRSYDTILFAEDSVRTGSIG
                     EQLGFAMQQCGWQGKYLLHAVDNTHLLHANVPELRKDQQLDAAALCKDILDYLKEKQA
                     "
     gene            complement(3174..3641)
                     /locus_tag="ACZPAX_05905"
     CDS             complement(3174..3641)
                     /locus_tag="ACZPAX_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent PAP2 family protein"
                     /protein_id="HIR1113465.1"
                     /translation="MPLLRAALSWNFVLVTAVCASLLAQLIKVLLNLFIFHRFIAERM
                     WGAGGMPSSHSATVCAMVVATGRYCGVSSPIFAIAAVLSIIVMYDAMGVRYETGEQAK
                     VLNRMFTEWMDQGFEQFQLPHGKKLKEMVGHTPIEVLTGAALGIVLGFAMPMV"
     gene            complement(3657..4550)
                     /locus_tag="ACZPAX_05910"
     CDS             complement(3657..4550)
                     /locus_tag="ACZPAX_05910"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015564115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HIR1113466.1"
                     /translation="MKHEDYKQQFTAWAQLTEARLAELCDTYLPQQSTLGQAARYSLL
                     GGGKRVRAVLTLAACQLCGADAADALDYACALEMLHCYSLIHDDMPCMDNDDFRRGRP
                     SCHKQFGEATALLAADALVTAAFEVIANAKLSAESRIEAVQLLSKGGGDRGMLYGQEL
                     DKRFETMRASESELLELHAHKTGALIIAAVELGCAAAGVKADTAIRKFLVRYAAEVGL
                     VFQIVDDILDVTSTTEELGKPVGSDADNDKTTFITLYGLQGAHEKAAHHNAAALAALD
                     ALGPKADFLRLMAAELLERKK"
     gene            complement(4540..4761)
                     /gene="xseB"
                     /locus_tag="ACZPAX_05915"
     CDS             complement(4540..4761)
                     /gene="xseB"
                     /locus_tag="ACZPAX_05915"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HIR1113467.1"
                     /translation="MKQPKSFEEGMDRLQGLLGQLQDESTPLADSVKLYAEAAGLIHY
                     CNTALDKAKLQVEEIDASLAPGGEVSHEA"
     gene            complement(4768..>5124)
                     /locus_tag="ACZPAX_05920"
     CDS             complement(4768..>5124)
                     /locus_tag="ACZPAX_05920"
                     /inference="COORDINATES: protein motif:HMM:NF014640.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HIR1113468.1"
                     /translation="CNLCYNRFNQLAAAQILQRRHSQLTHRERELAALQASVQSAAQR
                     RVQSAERQLQNAAAVAESLSPYAVLARGYTLTERQGVPCAVEALRSGETITVRGAKAA
                     AECRVEAVHDLETTGE"
BASE COUNT         1050 a         1574 c         1471 g         1029 t
ORIGIN      
        1 agccgcgtct ggccgaacag aacgatatca ttgatggcat cggcccgcca gccgtgggcg
       61 gggacctcgg cgtggagcag cccgcgattc acgagggtga actcctccgc tgccaagcgc
      121 tgcaattttg tgggcagttc ggcgacctcg caggtggcca gaaagcccat gcggcccagg
      181 ttcacaccca gaaccggctt gccgtgccgc atacaggaga gtccggcccg cagcaacgtt
      241 ccgtcgccgc cgatggtcac gacctggtcc gccgcgcgat aggcgtcagc ctccggcaaa
      301 aactgtaccc cggcaagcct gccctcaccc gcgaaggtct gcggcatcaa cacctgtacg
      361 cccaacccgg ccagcacagc agcagcctgc cgcgtaacgg caagccctgc gtccttggtg
      421 gggttcggaa tcagcaaaac tgacataggc gtcagattcc tttctcagtg cagattgggg
      481 gccttgtcca gcgtctcatg ggaggctgcc acgacctttt caatcaggcc ctccggcagc
      541 ggcaacggcg tgtcgcagtg ctggacgtac atcaaatatt caatgttgcc ctcgggcccc
      601 ttgatgggcg aaaaatccag gcccgcaggg gtgaaatggt ttgccatggc gtagccctcg
      661 gccatctgca agacctcgcg atgggtggcg gggtcacgca cgacaccctt tttgccgacc
      721 ttctcgcggc ctgcctcaaa ctgcggcttg acaaggcata cgccaacagc atccggcgtg
      781 cagattgcat ccgcaaccgg gaaaatatgc ttgagggaga taaacgaaac atcgacgctg
      841 aaaaacgcta ccggctcgga taaatcctcc ggcttcacgc tgcggatgtt cgttctctcc
      901 atattgataa cgcgctcgtc tgtgcgtagg ctccacgcca gctggccgta gccaacgtcc
      961 acggcataga ccttgaccgc gccgttttgc agcatgcagt cggtaaaacc gccggtagag
     1021 gcaccgatat ccatgcagac tttgccgtcc gggcgcatcg gaaagacctg catagccttt
     1081 tccagcttaa ggcctccgcg gctgacatag ccgatgtcgt gtccgcgcac ttcgactttg
     1141 gcgtcgggcg caatcatttc cccggctttg tcgactttct gctcgttgac aaagacaatg
     1201 ccggacatga tgagcgcctt ggcgcgctcc cggctctgca ccagaccatt ctggcacagg
     1261 tattgatcca ggcgaatttt gctcatgcct gtttctcctt taaatagtcc aaaatatcct
     1321 tgcacagggc cgccgcgtcc agctgctgat ccttgcgcag ttcgggcacg ttggcgtgca
     1381 gcaggtgggt attgtctacg gcgtgcagca ggtatttccc ttgccagccg cattgctgca
     1441 tggcaaagcc cagctgttcg ccgatgctgc cggtgcggac ggaatcctcg gcaaacaaaa
     1501 tcgtatcata gctgcgcagc gtgtcgcaca ggccgtcagg cagcgggaaa atctgcgtca
     1561 gcttgacgca gtcggtctgc acgccgttct gggcaagcag atccttggcc gcaataattt
     1621 cctcggtttc ggcaccgtag ctgaccaggg cgactttggc accctccaca ggttccagca
     1681 catcgtaccg cgcccctgtg cagggcaacg ccgcaagggc gggcttttcc tccccgcggg
     1741 catagcggat ggcacgcggg ccgcgggcct cacgcaccag ataggtcagc caatagcgaa
     1801 gctccgcata gttggacggc gcaaagaccg ggatatttac attcgagaaa aatgccacat
     1861 cgtagatgcc ctgatgggtc gcaccgtcgc cgggcacaag acccgcgcgg tctacggcga
     1921 aaagtacatc cagctgcatc aggctgacat catggatgat ttggtcgtag gcacgctgga
     1981 aaaacgtaga gtaaatcgca accacgggca aaagcccctg gctggcaagg cccgccgcaa
     2041 agctgacggc gtgttcttcc gccatgccga cgtcaaagaa gcggccagga tggcggtgct
     2101 tgaagaagtt gaggcctgtg ccgtacttca tggcagcggt gatgccacag acgcgcggct
     2161 cgtcatcgcc caaatccgct aacgcacagc cgaacgttga cgagaaggaa tccttcggcg
     2221 aaagctcggg gtcagtcaaa tggctcaaat cgatggacga taccgcgtgg aactcgccgg
     2281 ggttttcctc ggcgggtttc aggcctttgc ccttctgggt gacaatgtgc aggaacaccg
     2341 gggcatactg ctcccgcagg ttcgtcagcg tgtgggtcag catatccaca tcatggccgt
     2401 cgatgggccc gatgtactgg aagcccatct cctcaaacat cgtggatttg taaatgccgc
     2461 ggcgaatcag ctgcttgcca ctctgcaaca ccttgaccat ggcgttaccg gccaccggga
     2521 tgcgctccag cgttgtttcc atatgggcct tgacgtggga gtattttggg tcggtgcgca
     2581 gcttggtcag ataatttgcc atctggccga catttttgga aatcgacatc ttattgtcat
     2641 tcagaatgac aataagattg ttcagcttgc tgacattgtt catgccctcg tagaccatgc
     2701 cgccggtgaa agcgccatcg cccacgatga ccacgacttt gccaggctca ttcttgagct
     2761 ttttcgcccg ggcaatgccg atggcagtgg ataacgccgc actgccgtgc ccggcaatga
     2821 acgcgtcaca gtcgctctcc ttcgggctgg gaaagcccga aatgccgtct ttacggcgca
     2881 gctgggcaaa gcccgcgcgg cggccggtca gcagtttgtg ggtgtagcac tggtggccga
     2941 cgtcaaacag aagcttgtcc tgcgggaaat ccagcacgcg atgcagggca accgtcagtt
     3001 cgaccacgcc gaggttcgac gatagatggc cgccggtctc ggaaacactt ttaatcaaaa
     3061 actgccggat ttcctcacac agagcgggca tatcagtcat cggcaggttt tttatctccc
     3121 cgggcgcatt gatgcgctcc aacaaaggat agctcataga tgcacctcgt tttttacacc
     3181 atcggcatgg caaagcccag cacaatgcca agcgccgcgc ctgtcaaaac ttcgatcggc
     3241 gtatggccga ccatttcctt caactttttg ccgtgaggca gttggaactg ctcaaaaccc
     3301 tgatccatcc attcggtgaa catacgattc agaactttcg cctgctcacc ggtctcatag
     3361 cgcacgccca tggcgtcata catgacgata atggacagca cggccgcaat ggcaaaaatc
     3421 ggtgagctga cgccgcagta gcgccctgtg gcaacgacca tggcgcagac cgtggccgag
     3481 tgagagctgg gcataccgcc ggccccccac atacgctcgg caataaagcg gtggaagata
     3541 aacaggttga gcagcacctt aataagctgt gccaatagcg atgcgcagac cgccgtgacc
     3601 agcacaaaat tccaactcag cgcagcgcgc agcagtggca taggaaaatc acatccttat
     3661 ttcttacgtt ccagcagttc cgccgccatc agccgcagaa aatctgcctt tggcccaagc
     3721 gcatccagcg cggccagagc tgcggcgttg tgatgcgctg ctttctcatg ggcaccctgc
     3781 aagccgtaca gcgtgataaa ggtggttttg tcgttgtccg cgtcgctgcc gaccggcttg
     3841 ccgagttctt ctgtcgtaga ggtgacgtca agaatgtcgt caacaatctg aaatacaagg
     3901 ccgacttcgg ccgcatagcg aacaaggaat ttgcgaatag cagtatccgc cttaacgcct
     3961 gccgccgcgc agcccagttc cacagcggca atgatgagcg cgccggtctt gtgggcgtgc
     4021 agctctaaaa gttcgctctc actggcacgc atcgtttcaa aacgcttatc cagctcctga
     4081 ccgtagagca tcccgcggtc gccgccgccc ttggagagca gctgcacggc ctcaatgcgg
     4141 ctctcggcag agagcttcgc gttggcaata acttcaaacg cagctgtgac gagtgcgtcg
     4201 gcggccagaa gagccgtggc ctcgccaaac tgcttgtggc agctggggcg gccccggcgg
     4261 aaatcatcat tgtccatgca aggcatatcg tcgtggatga gcgaatagca gtgcagcatt
     4321 tccagcgcac aggcatagtc cagtgcgtcg gcggcatcgg ccccgcacag ctggcaggcg
     4381 gccagtgtca gtacggcgcg tacccgcttg ccgccgccca aaaggctgta ccgggcggcc
     4441 tggcccagtg tggattgctg cggcagatag gtgtcgcaca gttcggccag gcgggcttcc
     4501 gtcagctgcg cccaggcggt gaactgctgc ttataatcct catgcttcat gggatacctc
     4561 cccgcccggg gcaaggctgg cgtcgatctc ctcgacctgc agcttggcct tgtccagcgc
     4621 tgtattgcaa tagtggatca gaccggcagc ctccgcatac agcttgacgg aatctgccag
     4681 cggcgtggac tcatcctgca attgacccag caggccctgc aggcgatcca taccctcctc
     4741 aaaggatttg ggctgtttca tcgggtatca ctcccctgtt gtttctaaat catgcaccgc
     4801 ttccacgcgg cattcggcgg cggcctttgc accgcgcacc gtgatcgtct cgccggagcg
     4861 cagtgcctcc accgcgcagg gcacaccctg ccgctcggtc agtgtatagc cgcgtgcgag
     4921 aacggcgtag gggctcaggg attccgccac agcggcggcg ttttgcagct gccgctcagc
     4981 actctgcacg cggcgctgtg cggcgctttg cacagacgcc tgcagcgcgg ccaactcccg
     5041 ctcccggtgg gtaagctggc tgtgtcggcg ctgtaaaatc tgggcagcag ccagctgatt
     5101 aaacctatta tagcatagat tgca
//
LOCUS       DBMDSQ010000133         5047 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_4010_length_5047_cov_1.570562, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000133 DBMDSQ010000000
VERSION     DBMDSQ010000133.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 5047)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5047)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5047
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4010_length_5047_cov_1.570562"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            73..375
                     /locus_tag="ACZPAX_05925"
     CDS             73..375
                     /locus_tag="ACZPAX_05925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113469.1"
                     /translation="MMGWAGVTENVLHAQGEVATDFGETLSWEYLCYVRAHPLVWDVP
                     TCILYGEKDNLTSYETLAAFAQKVHAPITVMPSGEHWFHTEEQMSFLDEWVKGQCS"
     gene            complement(781..2082)
                     /locus_tag="ACZPAX_05930"
     CDS             complement(781..2082)
                     /locus_tag="ACZPAX_05930"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005534 - galactose binding [Evidence
                     IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HIR1113470.1"
                     /translation="MANTIELKQQIAQGEYDAAFTKLYGAAAVQEQRKRYTDLINEFE
                     KAYGTERTVRLYSAPGRTEIGGNHTDHNNGVVLAGSVNLDMVAVVSPNEENVIRVKSL
                     GFDKIDDVDVNILTPQPREAEHSASLIRGVAKGIVDAGGKVGGFDCYSTSNVLRGSGL
                     SSSAAFEVCIGAILRGEYNNNDMEKFSQVKIAQIGQYAENVFFGKPCGLMDQTACAVG
                     GVITIDFKDPSKPVVGQTAIDLAKHGFVMCISDTKGSHADLTDDYAAIRREMESVAAQ
                     FGKKVLRDVDEDEFYKAIPQLRKVVGDRAVVRAIHFYNDCRRAAQLCDAVRDDDFDTF
                     LKLIIEGGHSSFEFNQNAYSIKAPKEQGVPLALALSQKVLNGRGAWRLQGGGFAGTIQ
                     AFVPVDLLDAYKAAIDGCFGEGSCHVLNIRNYGAVPVTSDM"
     gene            2302..3171
                     /locus_tag="ACZPAX_05935"
     CDS             2302..3171
                     /locus_tag="ACZPAX_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HIR1113471.1"
                     /translation="MTINEMKQSYKQSYTDNVELSIFNCGHEPCLSGHTWGPGVRDHY
                     LIHLVVAGKGVYQVNGASYTLQAGDLFLAKPNQLITYAADETDPWEYYWVGFNGACAN
                     KLVQQTPFSDLHPVHHCKDPQTVREALYNIYLSRGTEPQNEALMTGYLYIFMAHLMKE
                     ARDAMPNVGSSSSQYVLAAIKFIQFNYSHDISVDDIAQAVGVSRSHLYRVFMSNVGQS
                     PIDYLTSYRISEACSLLKNSGLSIAEIAVSVGFFDQFYFSRVFKKVKGVPPSKYLVAL
                     EKEAQAAPQPINP"
     gene            3187..4554
                     /gene="rlmD"
                     /locus_tag="ACZPAX_05940"
     CDS             3187..4554
                     /gene="rlmD"
                     /locus_tag="ACZPAX_05940"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000154 - rRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HIR1113472.1"
                     /translation="MPFKKNQIVTLQIESLSNDGSGVAHQDGQAVFVPLTAPGDVTEV
                     RIVKPMKTYAFGRVEKLITAGPNRIQQDCPVAGPCGGCGLRHISYQAECAAKTQFVRD
                     AFARLGKLDVPVLDVLAAPAVDRYRNKVQLPVGTDENEHIVTGFFAGRSHRIVACSDC
                     KLQPAWMNQLAARTCELLEENGITAYNEETHTGRVRHLYMRQGWHSGQRLLCFVVNGN
                     GLPNEAEICAALQKEFDLTTILINRNSERTNVILGRKTRTAAGPGTIEDTLAGVPLHM
                     GVHEFYQVNTPAAEVLYAKARAYAGLKPDDFLLDLYCGMGTIGLSMLPDCQRLVGVEV
                     VPQAVEGAKETAARLGLGTEKADFRCQDAGAAAAQLAAEGARPDVIVVDPPRKGCDEA
                     TLTAIAEMAPRTVVMVSCNAATAARDTRWLAEHGYKAVEVQPVDLFPRTKHVECVVKL
                     TKDVK"
     gene            4571..>5047
                     /locus_tag="ACZPAX_05945"
     CDS             4571..>5047
                     /locus_tag="ACZPAX_05945"
                     /inference="COORDINATES: protein motif:HMM:NF025696.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HIR1113473.1"
                     /translation="MALPKYDELYSPFLTVIADGQVHNAKSVASDAAALLHLSTEDLS
                     EQLPSQRQTVFVNRLNWAKTYLKKAGLVDSPSRGNYVITQEGKRLLDSGVSITNEYLK
                     KNYASFAAFVGNGTQLSSASSPTQEDVKTPEEQMDALQQTINDTLADDLLSEIMRQT"
BASE COUNT         1068 a         1413 c         1469 g         1097 t
ORIGIN      
        1 tgacctacga gtacatcaat cgcatcgccg accggctaaa cctcgacctc gtaacgctca
       61 agtcgcccaa gtatgatggg ctgggctggg gtgacggaga atgtgctgca cgcgcagggc
      121 gaagtcgcca ccgattttgg cgaaaccctg tcgtgggagt atctctgcta cgtgcgggca
      181 cacccgcttg tgtgggatgt accaacctgc attttgtacg gcgaaaaaga caatctgacc
      241 tcctatgaaa cgttggccgc gtttgcgcag aaagtccatg cgcctattac ggtcatgcca
      301 agcggtgagc attggttcca caccgaagag cagatgtcgt ttttggacga gtgggtcaaa
      361 ggacaatgtt catgaaaaag ctgtatggga ttttgaaggc cgccctcggg tgtttcctcg
      421 gtgtgttttt gggcatgaca acgtacacct gctgggacta ccgcgcccac cccggactgt
      481 atgcgctgca atccgcaccg tggtatctga gtatacagat tcatgcggtg cttaccatag
      541 tggtttgcgg aatgctggca ctgggaatat ggatcttgcg aaagaaaatg taatcctgta
      601 gggcggcctg tcctcaggcc gccgcggagg gagcaaaatt tttccctata aatctacgga
      661 taagagggtg tctgccgtaa gcatgcgggc cgggcatgcc cggcccctac aggcgtaata
      721 taaaataccg taccggcaca tttctgcact ggtacggtgt tttttatgct ttatctaatt
      781 ttacatgtcg gacgtgacgg gcacggcacc gtagttgcgg atgttcagca catggcagct
      841 gccctcgccg aagcagccat cgatggccgc cttgtaggca tccagcagat ccaccggtac
      901 aaacgcctgg atggtacccg caaatccgcc gccctgcaag cgccaggcgc cgcggccgtt
      961 cagcactttc tggctcaggg ccaaggccag gggcacgccc tgctccttcg gggccttgat
     1021 gctgtacgcg ttctggttga actcaaagct ggaatggccg ccctcgatga tgagtttgag
     1081 gaaggtatca aaatcatcat cgcgcacggc gtcgcagagc tgcgcggcgc ggcggcagtc
     1141 gttgtagaag tggatggcac gcacgacggc gcggtcaccc acgaccttgc gcagctgcgg
     1201 gatggccttg tagaactcgt cctcgtcgac gtcgcgcagg acctttttgc cgaactgcgc
     1261 ggcgacgctc tccatttcgc ggcggatggc ggcgtagtcg tcggtcaggt cggcgtggct
     1321 gcccttcgtg tcactgatgc acatgacaaa gccatgcttt gccaggtcaa tggccgtctg
     1381 gcccacgacc ggtttggacg gatccttgaa gtcgatggtg atgacgccgc cgacggcgca
     1441 ggcggtctga tccatcaggc cgcagggctt gccgaagaac acattctcgg cgtactggcc
     1501 gatctgggca attttgacct ggctgaattt ttccatgtca ttgttgttgt attcaccgcg
     1561 caggatggca ccgatgcaga cctcgaacgc cgccgagctg gacaggccgg aaccgcgcag
     1621 cacattgctg gtggagtagc agtcaaagcc acccaccttg ccgcctgcat ccacaatgcc
     1681 cttggcaacg ccgcggatga gtgaggcaga atgctcggcc tcgcggggct gcggggtcag
     1741 gatgttgacg tcaacatcgt cgattttgtc aaagcccagg gacttgacgc ggatgacgtt
     1801 ttcctcgttc ggagagacaa cggccaccat gtccagattg acggaacctg ccagcacgac
     1861 accgttgttg tggtcggtgt ggttgccgcc gatctcggtg cggccggggg ctgagtagag
     1921 acggacggtg cgctcagtgc cgtaagcctt ttcaaattca ttgatcaggt cggtatagcg
     1981 cttgcgctgc tcctgcacgg cagctgcgcc gtacagcttg gtaaatgcgg cgtcgtattc
     2041 gccctgcgcg atctgctgtt tgagttcaat ggtatttgcc ataattccac tcacctttcc
     2101 tgtcgaacag ctctttttgt aattatgcac aaattgagcc ttatgttttt gggcaagcag
     2161 ccaaggagcc ggcggcctgc gtgtttcttg ttttcagtat agcaaatcct gcaaaaaatg
     2221 caaacagttt ttgcgccgca tttatggact tttgatgaat cctctgctat aatagagata
     2281 ctatgcaagc gaggtgcagc catgaccatc aatgagatga agcaatccta caagcagagc
     2341 tacacggaca atgttgagct ctcgattttt aactgcgggc acgagccctg cctgtcgggc
     2401 cacacgtggg gccccggtgt gcgcgaccac tacctgatcc acctggttgt ggcgggcaag
     2461 ggcgtctacc aggtcaatgg tgcatcgtac actttgcagg cgggcgatct gtttttggcc
     2521 aaacccaacc agcttatcac ctacgctgcc gacgagaccg acccttggga gtactattgg
     2581 gtcggtttca atggcgcgtg cgccaataag ctggtgcagc agacaccgtt ctcggacctt
     2641 cacccggttc accactgcaa ggacccgcag accgtgcgcg aggctttgta taatatctat
     2701 ctttcccgcg ggaccgagcc gcagaacgaa gccctgatga ccggctatct gtacattttt
     2761 atggcgcacc tgatgaagga agcccgcgac gccatgccga acgttgggtc gtccagcagc
     2821 cagtatgtgc tagccgccat caagtttatc cagttcaact actcccacga tatctcggtg
     2881 gacgacattg cccaggccgt cggcgtcagc cgtagccatt tgtaccgcgt gttcatgtcc
     2941 aacgtcgggc aaagccccat cgactacctg acgagttacc gcatcagcga ggcgtgcagc
     3001 ctgttgaaaa attccgggtt gtcgattgcc gaaatcgccg tatctgtggg attttttgac
     3061 cagttctatt tctcccgcgt ctttaaaaag gtcaagggtg tgccgccgag caagtatctt
     3121 gtcgcattgg aaaaagaagc acaggctgcg ccgcagccca tcaatccgta actacaagga
     3181 gcaactatgc cttttaagaa aaaccagatc gtaactttgc agatcgagtc tctttccaac
     3241 gacggcagcg gcgtggccca tcaggacggc caggccgttt ttgtgccgct gactgccccc
     3301 ggcgatgtga ccgaggtgcg cattgtcaag ccgatgaaaa cctacgcctt tggtcgcgtg
     3361 gaaaagctca tcaccgccgg gccgaaccgc attcagcagg attgcccggt ggccgggcct
     3421 tgcggcggct gtgggttgcg gcatatcagc taccaggccg agtgcgccgc caagacgcag
     3481 tttgtgcgcg acgcctttgc ccgcctgggc aagctggatg tgccggtgct ggatgtgctg
     3541 gccgcccctg cggtggaccg ttaccgcaac aaagtccagc tgccggtggg caccgatgaa
     3601 aatgagcata tcgtgaccgg gttctttgcc gggcgcagcc accgcattgt ggcatgcagc
     3661 gactgtaagt tgcagcccgc gtggatgaac cagcttgccg cccgcacctg cgaattgttg
     3721 gaagagaacg gcatcaccgc ctacaacgag gaaacccaca ccggccgtgt gcgccatttg
     3781 tatatgcgtc agggctggca cagcggccag cggttgctct gctttgtggt gaacggcaac
     3841 ggcctgccaa atgaggctga aatttgcgct gccttgcaaa aagaatttga tttgacgacg
     3901 attttgatta accgcaacag cgagcgcacc aacgtaattt taggccgcaa gacccgcacc
     3961 gccgccggcc ccggcactat tgaggataca ttggccgggg tgccgctgca catgggcgtg
     4021 cacgagtttt accaggtcaa tacccccgcc gccgaagtac tgtacgccaa ggcgcgggcg
     4081 tatgccgggc tgaagcctga cgactttttg ctggacctgt actgcggcat gggcacgatc
     4141 gggctttcga tgctgccgga ctgccagcgc cttgttggcg tcgaggtcgt gccccaggct
     4201 gtggaaggtg ccaaggagac cgctgcacgg ctgggacttg gcacagaaaa ggcagacttc
     4261 cgctgccagg atgccggtgc cgctgcggcc cagctggccg ccgagggggc ccgccccgat
     4321 gtaattgttg tggatccgcc gcgcaagggc tgcgacgaag ccacgctgac cgccattgcc
     4381 gaaatggccc cgcgcaccgt ggtcatggtc agctgcaacg ctgccaccgc cgcccgcgat
     4441 acccgctggc tggccgagca tggctataag gccgtggagg tgcagccggt ggatttgttc
     4501 ccgcggacaa agcacgtgga gtgtgtagtg aaactaacta aagatgtgaa atgagaggga
     4561 atttgtaaag atggctttac ctaaatatga tgagctgtac agcccctttc tcactgtaat
     4621 tgcagatggc caggttcata atgcaaagtc tgttgcatct gatgccgctg ctctgttgca
     4681 cttgtcaacc gaggatttgt cagagcaatt acccagccag cgccaaacgg tttttgttaa
     4741 tcggttgaac tgggcaaaaa cttatttgaa gaaagcgggc cttgtagatt ccccaagtcg
     4801 cggcaactat gtgataacac aagagggcaa acggctgttg gatagtggcg tttccatcac
     4861 caacgagtac ttgaaaaaga actatgcgtc gtttgccgcc tttgtcggca acggaaccca
     4921 actttccagc gcatcctccc caacacagga agacgtgaaa acgccagaag agcagatgga
     4981 tgccctgcaa cagaccataa acgatacgct tgccgatgac cttctatccg aaatcatgcg
     5041 acagaca
//
LOCUS       DBMDSQ010000134         5012 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_4043_length_5012_cov_1.789249, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000134 DBMDSQ010000000
VERSION     DBMDSQ010000134.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 5012)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5012)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5012
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4043_length_5012_cov_1.789249"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="ACZPAX_05950"
     CDS             <1..252
                     /locus_tag="ACZPAX_05950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113474.1"
                     /translation="SAIPSAPLRDQPKAPRPQRSRAQHSAVPNEPGLDLISRRPPKQK
                     FANFEDYLAAHGGMTVPLPLAAADAPEAEKPAPAAEATE"
     gene            249..1064
                     /locus_tag="ACZPAX_05955"
     CDS             249..1064
                     /locus_tag="ACZPAX_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005934691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative RNA methyltransferase"
                     /protein_id="HIR1113475.1"
                     /translation="MTPETAAALRCPVCGGPLLLTGRSLRCPKAHSFDLAKEGYAYLL
                     PMQKKHTADPGDGKAMVRARRAFLSAGHYAPLMATLAEVCAALPHDHIVDAGCGEGSY
                     DKYLYETLGAPQIVGFDLSKETVRLASKLLPGAAFCVGGSFCAPVRDGWADLLLNIFS
                     PMAEAEFARMLRPGGHLVYAVPTARHLFGLKEVLYERPYENEVRQTEYDGFTFVKAVE
                     SEAVLTLEGQAVQDLFAMTPYYWNTPANGAARLAACGSLTTEIGFRFLVYQKM"
     gene            complement(1217..1399)
                     /locus_tag="ACZPAX_05960"
     CDS             complement(1217..1399)
                     /locus_tag="ACZPAX_05960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113476.1"
                     /translation="MFDEFGPHGAEEEREELWTDLSEVPDEQYNEDDPAETTQEVIVP
                     PAQEPYGPGVVPPEEI"
     gene            1486..2361
                     /locus_tag="ACZPAX_05965"
     CDS             1486..2361
                     /locus_tag="ACZPAX_05965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113477.1"
                     /translation="MRSHRGIFWYSLGLTLLLLLPMVLTVAFFADQYQKQQLLRQASA
                     ADSTLRIEPGAQGVYRLLLIVQQEEPAFVLARADGPKQAVTLCALPGSLLVNAPAGTT
                     TLSECALSAGAGRAAQLLCGTVATGETAAPELHYIAATPATWAACAGKNAAVRFDTAA
                     LLTPTARERLGYGDDPVAACTAAQAGELIADLQGALAGAGESADARAAVWAAFARQDP
                     ALLAAFPTGLRSQSARLLTDLLAADLSALEDTLTYLSTRPALAVDYVTAETAKAKGGV
                     CLTDEGMAAVRDLLS"
     gene            2589..2738
                     /gene="rpmG"
                     /locus_tag="ACZPAX_05970"
     CDS             2589..2738
                     /gene="rpmG"
                     /locus_tag="ACZPAX_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004402360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HIR1113478.1"
                     /translation="MTVKITLACTECKQRNYNKEKNKKNSPDRLEMKKYCRFCKKHTL
                     HRETK"
     gene            2755..3048
                     /gene="secE"
                     /locus_tag="ACZPAX_05975"
     CDS             2755..3048
                     /gene="secE"
                     /locus_tag="ACZPAX_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HIR1113479.1"
                     /translation="MADKKAATAAAQTQSDKPKKNDGVKKVSFFGKVKNFFKGIGKYF
                     KDTKSELKKVVWPSKHDVKNNTITVIVVVLIAAVVLIALDLIFGGAIHLLIGA"
     gene            3073..3612
                     /gene="nusG"
                     /locus_tag="ACZPAX_05980"
     CDS             3073..3612
                     /gene="nusG"
                     /locus_tag="ACZPAX_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HIR1113480.1"
                     /translation="MAEQANWYVAHTYSGYENKVATDLMTMVENRHLQDLICDVKVPT
                     ETRIEEVFDKRGNKTGEKEVQSKLYPGYVFVKMVMNDNTWYIVRNTRGCTGFVGPESK
                     PEPLTEAEVAKMGVETTTDIEVEYKVGDTVEITAGPMEGSVGTVEEIDIPARKVRVKI
                     TMFGRELPAELELHQVKLF"
     gene            3760..4185
                     /gene="rplK"
                     /locus_tag="ACZPAX_05985"
     CDS             3760..4185
                     /gene="rplK"
                     /locus_tag="ACZPAX_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HIR1113481.1"
                     /translation="MAQKITGYIKLQIPAGKATPAPPVGPALGQKGVNIMAFTKEFNE
                     RTKNQMGMIIPVVITVYADRSFSFITKTPPAPVLIKKAAGIDTASGVPNKNKVGSITL
                     AQAEEIAKTKMPDLNAASLEAAVSMIKGTARSMGVTVEG"
     gene            4204..4896
                     /gene="rplA"
                     /locus_tag="ACZPAX_05990"
     CDS             4204..4896
                     /gene="rplA"
                     /locus_tag="ACZPAX_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005944523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HIR1113482.1"
                     /translation="MKHGKKYIDAAKLIENNKTYDPSEALELCCKTATAKFDETVELH
                     VRLGVDSRHADQQVRGAVVLPNGTGKNVRVIAIAKGDAAKAAEAAGAQEVGDDDLIAK
                     IAGGYLDFDVLVTTPDMMGRVGRLGKVLGPRGLMPNPKAGTVTPDVGKAVTDAKAGKI
                     EYRLDKQNIIHVPIGKASFGAEKLMTNLDTVLDAIAKAKPAAAKGQYFKSATIATTMG
                     PGVRVATNKYGV"
BASE COUNT         1033 a         1515 c         1503 g          961 t
ORIGIN      
        1 tcggccatcc ccagcgcccc gctgcgtgac cagcccaagg caccgcgccc gcagcgcagc
       61 cgcgcccagc actccgccgt gcccaacgag ccgggtctgg atctgatttc ccgccgcccg
      121 ccgaagcaga agttcgcgaa ttttgaggat tacctcgcgg cccacggcgg tatgaccgta
      181 ccgctgccgc tggcagctgc ggacgcgccg gaggcagaga agcccgcccc agctgccgag
      241 gccaccgaat gacccccgag actgcggcag cactgcgctg cccggtatgc ggcgggccgc
      301 tgctgctgac cggccgcagc ctgcgctgcc ccaaggccca cagctttgac cttgccaaag
      361 aaggctacgc ctacttactg ccgatgcaga aaaagcacac tgccgacccc ggcgacggca
      421 aggccatggt tcgcgcccgc cgcgcgtttt tgtcggccgg gcactacgcc ccgctgatgg
      481 cgacgctggc cgaggtctgt gccgcgctgc cgcatgacca tattgtggac gcgggctgcg
      541 gcgagggcag ctatgacaag tatttatatg agacgctggg tgcgccgcag attgtcggct
      601 ttgatttgtc caaggagacc gtgcggctgg cgtccaagct cctgcccggt gcggcgttct
      661 gtgtcggcgg cagcttttgc gccccggtgc gggacggctg ggccgacctg ctgctcaaca
      721 tcttctcgcc catggccgaa gctgaatttg cccggatgct gcgtccgggt gggcatctgg
      781 tttacgccgt gcccaccgcg cggcacctgt tcggcctgaa agaggttttg tacgagcgcc
      841 cctacgaaaa cgaggtgcgc cagacagaat atgacggttt tacgttcgtt aaggctgtcg
      901 aatccgaggc cgtgctgacg ctggaaggcc aggccgtgca ggatctgttt gccatgacgc
      961 cgtattattg gaacacgccc gccaatgggg ccgcacggtt agcggcgtgc gggagcctga
     1021 ccaccgagat cgggttccgc tttttggtgt atcaaaaaat gtaaagcctt ccccctgtgg
     1081 ggggaaggtg gccgcgcgaa gcacagccgg atgaggggcg agtttgccgt tgccgaactg
     1141 taaaagggga ggccaagcag actcgcccct catcagtcac cttcggtgac agcttctccc
     1201 acgggagaag ccatttttaa atctcttccg gcggcacaac gcccgggcca tacggctctt
     1261 gggcgggcgg tacgatgacc tcctgcgttg tctccgcggg gtcatcctcg ttgtactgtt
     1321 cgtcaggcac ttcgctcaaa tccgtccaca gctcctcgcg ctcttcctct gcgccgtggg
     1381 ggccaaattc atcaaacatt cgcaaacact cctttataat agtgtataag tgcggcccgg
     1441 ccggcctaca ctctgggcat agtctttgca aggggtgtgt aggttatgcg ttcacatcgc
     1501 gggattttct ggtacagcct ggggctgacg ctgctgttgt tgctgcccat ggtgctgacc
     1561 gtggcatttt tcgccgacca ataccagaaa cagcagcttt tgcgccaggc atcggcagcg
     1621 gacagcacgc tgcgcattga accgggtgcg cagggcgttt accggctgct gctcatcgtc
     1681 cagcaggagg aaccggcctt tgtgctggcc cgggcagacg gccccaagca ggcagtgacc
     1741 ctctgtgcgc tgcccgggtc actgctcgtc aacgcgcctg cgggcacaac gacgctgtcc
     1801 gagtgcgcgt tgagcgccgg agccgggcgt gccgcgcagc tgttgtgcgg caccgtggcg
     1861 actggcgaga cggccgcgcc ggagctgcac tatatcgcgg ccacgcccgc cacctgggcc
     1921 gcctgtgcgg gcaagaatgc tgccgtgcgg tttgacacgg cggcactgct tacccccacc
     1981 gcccgggagc gcctgggtta tggcgacgac ccggtggccg cctgcaccgc cgcccaggca
     2041 ggggagctga ttgccgactt gcagggcgca ctggccgggg caggggagag cgccgacgcc
     2101 cgcgccgccg tctgggccgc ctttgcgcgg caggacccgg cgctgctggc agcgttcccc
     2161 accggactgc gcagccagag cgcccgcctt ttgaccgacc tgctggccgc cgatctcagc
     2221 gcgttggagg atacgctgac ctatctcagt acccgccccg cgctggccgt ggattacgta
     2281 accgccgaaa ctgctaaagc caaaggcggg gtatgtttga cggatgaggg aatggcagcg
     2341 gtgcgtgatt tgttatcgta ggggtcggct ccccaatgac ctgcgggcgg gcattgcccg
     2401 cccctacgtt gcgttcctcg ccacggcgtc aaaaaattac aaaaaaattt gcaaaaccgc
     2461 ttgacgtaaa cccgcttgtg tgttatcatg atagcacctc ttttgcgggt ttatttttta
     2521 tgcccttttt tctagggcgc ctgcaagcgc gagggaggct tattaaccga taatggaggt
     2581 gccgaacaat gactgtcaag atcaccctgg cttgcaccga gtgcaagcag cgcaactaca
     2641 acaaggagaa gaacaagaag aactctcctg accgtcttga gatgaagaag tattgccgtt
     2701 tctgcaagaa gcataccctt caccgcgaaa ccaagtgagg ataggagctt agagatggca
     2761 gacaaaaaag cagccaccgc tgcagcccag actcaaagcg acaagcccaa aaagaacgac
     2821 ggcgtgaaga aagtttcttt cttcggtaag gtcaagaact ttttcaaagg aatcggcaag
     2881 tatttcaaag atacgaagag cgagctgaaa aaggtcgtgt ggcccagcaa gcatgatgtc
     2941 aagaacaata cgatcaccgt tatcgtggtc gtgctgatcg cggcggtcgt gctgatcgcg
     3001 ctggatctca tctttggcgg cgccattcac ctgttgatcg gcgcctgagc gcgttgagcg
     3061 gaggcaaaga ccatggctga gcaagcaaac tggtatgtag cacataccta ttccgggtac
     3121 gagaacaagg tcgctacgga tctgatgacg atggtggaaa accgtcatct gcaggatctg
     3181 atctgcgacg tcaaggttcc caccgagacc cgcattgaag aagtttttga caaacgcggc
     3241 aacaagaccg gcgagaaaga ggttcagtcg aagctctatc ccggttacgt tttcgtcaaa
     3301 atggtcatga acgacaacac ctggtacatc gtgcgcaaca cgcgcggctg caccggcttt
     3361 gtcggcccgg agtccaagcc cgagcccctg accgaggcgg aagttgccaa gatgggcgtc
     3421 gaaaccacga ccgacattga ggtcgagtac aaggtcggtg atactgtcga gatcactgca
     3481 ggcccgatgg agggttccgt cggcactgtg gaggagattg atattcctgc gcgcaaggtg
     3541 cgcgtaaaga tcaccatgtt tggccgcgag ctgcccgcag aactcgagct gcatcaggtc
     3601 aagctgttct gattttcaga acaccctagt ttccggtaaa gaccgcgaaa ggaaattttt
     3661 cctgggtacg gtttttatag ggcgtgcgga ggtcgtgcgc ccgtgggagg tctgcaccgc
     3721 aggccgcaac tcaccacaaa atttggaggt gcaagtatta tggcacagaa aatcactggc
     3781 tacatcaagc tgcaaatccc cgccggcaag gcaactccgg ctccccctgt tggtcctgct
     3841 ctgggtcaga agggcgtcaa catcatggcg ttcaccaagg agttcaacga gcgtaccaag
     3901 aaccagatgg gcatgatcat ccccgtcgtc atcacggttt acgctgaccg ctccttctcc
     3961 ttcatcacca agactcctcc ggcgcccgtt ctgatcaaga aggctgccgg catcgacact
     4021 gcttccggcg tgcccaacaa gaacaaggtt ggctccatca cgctggcaca ggccgaggaa
     4081 atcgccaaga ccaagatgcc cgacctgaac gccgcttctc tggaagctgc tgtcagcatg
     4141 atcaagggca ccgcccgcag catgggcgtt accgttgagg gctgaatagg agggtaatac
     4201 gcaatgaaac acggcaagaa gtatatcgac gctgcgaagc tgatcgagaa caacaagact
     4261 tacgatccca gcgaagccct ggagctctgc tgcaagaccg ctaccgctaa gttcgacgag
     4321 accgttgagc tgcacgtccg cctgggcgtt gacagccgcc atgctgacca gcaggtccgc
     4381 ggcgctgttg tcctgcccaa cggcaccggc aagaacgtcc gcgttatcgc catcgccaag
     4441 ggcgacgctg ccaaggctgc tgaggctgca ggcgctcagg aggtcggtga cgacgatctg
     4501 attgctaaga tcgccggcgg ttacctggac ttcgacgtcc tggtcaccac ccccgacatg
     4561 atgggccgcg ttggccgtct cggtaaggtc ctgggccccc gtggtctgat gcccaacccg
     4621 aaggccggca ctgttacccc cgacgtcggc aaggctgtta ccgacgctaa ggccggtaag
     4681 atcgagtacc gcctggacaa gcagaacatc attcatgtgc ccatcggcaa ggcctctttc
     4741 ggtgccgaga agctgatgac caacctggac accgtcctgg atgccatcgc caaggctaag
     4801 cctgccgctg ccaagggcca gtacttcaag agcgctacca tcgccaccac catgggcccc
     4861 ggcgtccgcg ttgctaccaa caagtatggt gtttaattga gcgcgttgcg cataggcgcg
     4921 ctcaattaaa cgacgcaaag cggcgttcct ctgttacccg cccatgaaca tgggcaatga
     4981 attaagtaga gaaattccgc tttttctatt ga
//
LOCUS       DBMDSQ010000135         5003 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_4055_length_5003_cov_1.685027, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000135 DBMDSQ010000000
VERSION     DBMDSQ010000135.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 5003)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5003)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5003
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4055_length_5003_cov_1.685027"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            72..1949
                     /locus_tag="ACZPAX_05995"
     CDS             72..1949
                     /locus_tag="ACZPAX_05995"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: protein motif:HMM:TIGR01427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIABC"
                     /protein_id="HIR1113483.1"
                     /translation="MRIANYLRPDCVALRQRADSLQGAVQQLVALLDGAKVLTDTAAF
                     AADVRARLELGGVCVGNGLAIPHAKSTAVEKLQLAALTLDPPLPCDTPDGQPLEMLFL
                     IAAPAEANDLHVQVLAELATLFLDTDFCASLRQSETPEAFCRAIARREAQDDPAAPPP
                     TPTNEPGYRLLGVTACPTGIAHTYLAAEALQQAAQTRGLTIKVETNGAGGVGNELTED
                     EIQAADCIIVAVDRSISLARFVGKRLVYTSAGEAVRNADALLEKAVSGKASIYRGGHA
                     FRTNDWKELGREYYGHLMSGISHMLPFVVAGGVMLALSLLLQHLGGPANWSTMMTNVG
                     NAALSMMYPILSAFIAYSIADRPGLMPGLMGGYLAQTGMTTATKLSWIPSGFWGGLIA
                     GFAAGLVVRLLNRLFRRIPQDLDHIKTGLLVPLLSLLFVGWLMLMVVNPPLGRFNRWM
                     SLALAKLQGGSRLVLGAVLGALMATDYGGPINKAAYVTGTLAVTNSQYDLMAAVMAGG
                     MIPPLGLALACLLFPTRFTKAERRTVPQNLVMGASFVTEGALPFALRDPLRVVPSCMA
                     GSALAGFLTVLFGCGCPAPHGGLFLLAVIGNPLGFLAALAAGSLLTTLLVGMLKKPVK
                     S"
     gene            2075..3826
                     /locus_tag="ACZPAX_06000"
     CDS             2075..3826
                     /locus_tag="ACZPAX_06000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113484.1"
                     /translation="MEQVKTFYRRYRWLFLAAAYLVVFAVGVLYLRVTISIPPEGDDK
                     LTLNRWMYDFQRMPFWQYLLQDLQGRLRYFTLQEVRFFPFHYPNAVALLFYGSLSHYR
                     LYIIAWTGAAAFLVSRVAARFGSGDALALGGFTLAMAAAPIWNEGMYSYYAVPQRALF
                     WAMAAWLCLFGWENSHRRGWAAAAALLSFVACGTYEIGYMFAALAFVVWCLRARSVKA
                     GLFSTAPALGGMGVALIFHMLCGRSGGTGNEVSLDIPQVLRVLVQQMAASLPGLNSRI
                     LQEDCGVLSNGDRLWPLVLGVLAGLLLFFAVPREKLNLKMLGAAAGFGLAVWALPALL
                     LALSARYQAPGAITWKWGYIPAAASSIGFALVVAVLLAALAGACRKLPQILSILARLG
                     LTAALAAGLCLNCSYLRGVVRTHHAENLDNYQFFVRTIQSGLADAVQPGDLILCNENV
                     WDSNADAETAFFSRFTGRDLHAAVLGTTYDAPAGNTYAYQTYRKYGGYDLAWCGRLQS
                     ADSDLMDGVQVYVQSACVPDNAVIKYKVRLADGTEEARAICLLDCTQTPRDANGDYLA
                     TVEDSGIVNAKLMIWDG"
     gene            3845..>5003
                     /locus_tag="ACZPAX_06005"
     CDS             3845..>5003
                     /locus_tag="ACZPAX_06005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113485.1"
                     /translation="MKHFFAFCKRNRLFAALLAVELAVLCVLAAGLFGAPYKLTMTPD
                     LFENPFPDIASNEGDHFQVWNQTNFQTSDPLDFPSAGQAVRSGAYEVTVQYFSCQTPD
                     APTFNMLNSAGTLSFSSAMVPSAVSADTLRLDDCHRTLTTRLWVSSGAQLNDLTATLH
                     YNGQGQLYLYAITLTEQPIYRVTRLLGFALFFAVVDFLLWLLFAKTGEEGAASRRALR
                     LPLALIAITAFACLPLFSNYLYFGHDMDYHLQRISAMAAELSYGQFPVRFTTTTLNGY
                     GYANPLCYCELFLLLPALLYNLWLPLRTCYQIYLFAVTLATCAIAYYSFAKIAASRRL
                     GLLGALLYTLSCYRLVCVYTRAAAGEFTAMAFFPLVLAARRLRSRCSNSAIF"
BASE COUNT          762 a         1643 c         1575 g         1023 t
ORIGIN      
        1 actaattttt caagctctca tagcggttcc gttgcggcgg aaccgctttt ttagtaaacg
       61 gaggaacgac gatgcgcatt gccaactacc tgcggccgga ttgtgtcgca ctgcgccagc
      121 gggcggattc cctgcagggc gcggtgcagc agcttgtggc gctgctggat ggggccaaag
      181 tcctgacgga cacggcagcc tttgcggcag atgtccgcgc ccggctggaa ctgggcggcg
      241 tctgcgtggg caatggactg gcgatccccc acgccaagag caccgcggtg gaaaaattgc
      301 agctggccgc attgacgctg gatccaccgc tgccctgcga tacgcccgac ggccagccgt
      361 tggagatgct gttcctcatc gctgcccccg ccgaggccaa cgacctgcat gtgcaggtgt
      421 tggccgagct ggcaacgctg tttttggata ccgatttctg cgccagtctg cgccagagcg
      481 agacgcccga ggccttctgc cgcgccattg cccggcgcga agcccaggac gacccggccg
      541 cgcccccgcc gacgcccacc aacgagccgg gctaccggct gctgggtgtg acggcctgcc
      601 ccaccggcat tgcgcacacc tacctggcgg ccgaggcctt acagcaggcc gcccagaccc
      661 ggggcctgac catcaaagtg gagacgaacg gcgcgggcgg cgttggcaat gaactgaccg
      721 aggacgagat ccaggccgcc gactgcatca tcgtggcggt agaccgctcg atctcactgg
      781 cgcggtttgt gggcaagcgg ctggtctaca cctcggcagg ggaggccgtg cgcaacgcgg
      841 atgccctgct ggaaaaggcc gtctccggca aagcatccat ctaccgcggc ggccacgcct
      901 tccgcaccaa cgactggaag gagctgggcc gcgagtacta cggccacctg atgagcggca
      961 tctcccatat gctgcccttt gtggtggcgg gtggcgtgat gctggcactc tcgctgcttt
     1021 tgcagcacct gggcggcccg gcgaactggt ccacgatgat gacgaacgtc ggcaacgccg
     1081 cgctctccat gatgtacccg attctgtccg cgttcatcgc gtacagcatt gccgaccggc
     1141 ccggcctgat gcccggcctg atgggcggct acctggccca gaccggcatg acaacggcca
     1201 ccaaactcag ctggatcccc agcgggttct gggggggcct cattgcgggc tttgccgccg
     1261 ggctggtggt acgcctgctg aaccgactgt tccgccgcat cccccaggat ctggaccaca
     1321 tcaagaccgg gctgctggtg ccgctgttga gcttgctttt tgtcggctgg ctgatgctta
     1381 tggtcgtcaa cccgccgctg gggcggttca accgctggat gtcgctggcg cttgccaagc
     1441 tgcagggcgg cagccgcctg gtgctgggtg ccgtgctggg tgcgctgatg gccactgact
     1501 acggcgggcc catcaacaag gcggcctacg tcaccggcac gctggctgtg accaacagcc
     1561 aatatgacct catggccgcc gtcatggcgg gcggtatgat ccccccgctg gggctggcac
     1621 tggcctgcct gctgttcccg acgcggttca ccaaggccga gcgccgcacc gtgccgcaga
     1681 accttgtgat gggcgcgtcc tttgtgaccg agggtgcgct gccctttgcc ttgcgcgacc
     1741 cgctgcgggt cgtaccctcc tgcatggcag gcagtgcgct ggccgggttc ctgaccgtac
     1801 tgtttggctg cggctgccct gcgccccacg gcggattgtt cctgcttgcc gtcataggca
     1861 atccgctggg ctttttggct gccctggcgg ctggcagtct gctgaccaca ttgttggtcg
     1921 gtatgctgaa gaagcctgtg aaatcgtaaa tcttcgtttt cttcgttcct gccgcagaat
     1981 atccgctttc cacccccgct gtgcgttgcg tgcagcgggg gtttatgcta tactatgaat
     2041 atatgtgtgt caaaaagagg aggcctttac ccccatggaa caggtcaaga cgttttaccg
     2101 gcggtaccgc tggttatttt tagcggcggc ctatctggtg gtgtttgcgg tgggcgtgct
     2161 gtatctgcgc gtcaccatca gcatcccgcc ggaaggggac gacaaattaa cgctgaaccg
     2221 ttggatgtac gacttccaac ggatgccctt ctggcaatac ctattgcagg acttgcaggg
     2281 gcggctgcgg tatttcaccc tgcaggaggt tcggttcttt ccgttccact accccaacgc
     2341 ggtagcgctg ctgttttacg gcagtttgag ccattaccgg ctctacatca ttgcctggac
     2401 cggggcggcg gcgttcctgg tcagccgggt ggccgcgcgg ttcggcagcg gggatgccct
     2461 ggcgctgggc ggctttacgc tggccatggc ggcggccccc atctggaacg agggtatgta
     2521 cagctactac gccgtgcccc agcgggcgct gttctgggcc atggcggcct ggctctgcct
     2581 gtttgggtgg gagaattccc accgccgcgg ctgggctgca gcggcagcgc tgctctcctt
     2641 cgtcgcctgc ggtacttatg aaattggcta tatgttcgcg gcgctggcct ttgtggtttg
     2701 gtgcctgcgt gcccgcagcg tcaaagccgg gctgttcagc accgcccccg cgctgggcgg
     2761 catgggcgtg gcgctcatat tccatatgct gtgcggtcgc agcggcggca ccggcaacga
     2821 ggtctcactg gatatccccc aggtgctgcg cgtgctggtg cagcagatgg ctgcgtctct
     2881 ccccgggctg aactcccgca ttttgcagga ggattgcggc gtactctcca acggcgaccg
     2941 gctgtggccg ctggtgctgg gcgtgctggc cggactgctg cttttcttcg ccgtgccgcg
     3001 cgaaaaactg aacctcaaaa tgttgggtgc cgccgccggg tttggcctgg cggtctgggc
     3061 gctgcccgca ctgctgctgg cgctgtccgc ccgctaccag gctcccgggg ccatcacctg
     3121 gaagtggggc tacatccccg ccgccgcgag ctccatcggc tttgcgctgg tggtggccgt
     3181 gctgctggct gcgctggcgg gtgcctgccg caagctgccc caaattttgt ccattctggc
     3241 gcggctgggc ctgaccgccg cgctggccgc cgggctttgc ctgaactgct cctacctgcg
     3301 gggcgtcgtg cgcactcacc acgccgagaa tctggataac taccagttct ttgtgcgcac
     3361 catccaatcc ggcctggcag atgccgtcca gcccggggat ctcattctct gcaatgagaa
     3421 tgtctgggac agcaacgccg acgccgagac ggcctttttc agccggttca ccgggcgcga
     3481 cttgcacgct gccgtgctgg gcacgaccta cgatgccccc gccgggaaca cctacgccta
     3541 ccagacctac cgcaagtacg gcgggtacga cctggcctgg tgcggcaggc tgcaaagcgc
     3601 cgacagtgac ctgatggacg gcgtgcaggt ttatgtgcag agcgcctgcg tgcccgataa
     3661 tgcagtcatc aagtacaaag tccgcctggc cgacggcacc gaggaagccc gcgccatctg
     3721 cctgctggac tgcacccaga ccccgcggga cgccaacggc gactaccttg ccaccgtgga
     3781 ggacagcggt atcgtgaatg ccaagctcat gatctgggac ggctaactaa aagaggattt
     3841 acggatgaaa catttctttg ctttctgcaa gcgcaaccgg ctgttcgctg ccctgctggc
     3901 ggtggagctg gccgtgctct gcgtgctggc ggccgggctg ttcggtgcgc cttacaagct
     3961 gacgatgacc cccgacctgt tcgagaaccc cttcccggac attgcctcca acgaggggga
     4021 tcacttccag gtgtggaacc agacgaactt ccagaccagt gacccgctgg atttcccttc
     4081 tgccgggcag gcggtgcgat ccggcgcgta tgaggtgacg gtgcagtatt tctcctgcca
     4141 gacgcccgac gcgcccacct tcaatatgct caattccgcc ggtacgctga gcttttccag
     4201 cgccatggtt ccctcggcgg tcagcgccga caccctgcgg ctggatgact gccaccgcac
     4261 cctgactacc cggctgtggg tcagcagcgg cgcacagctc aacgatttga ccgccacgct
     4321 gcactacaac ggccagggcc agctctatct ctacgccatc accctgaccg agcagcccat
     4381 ctaccgcgtc acccggctgc tgggctttgc gctgttcttc gcggtcgtgg acttcctgct
     4441 ctggctgctg tttgccaaaa caggggagga gggcgctgcc agccgccgtg ccctgcggct
     4501 gccgctggcg ctcatcgcca tcacggcgtt tgcctgcctg ccgctgttca gcaattatct
     4561 ctacttcggc cacgatatgg actaccacct gcagcgcatt tctgccatgg cggcagagct
     4621 gtcctatggg cagttcccgg tgcggttcac caccacgacg ctgaacggct acggctacgc
     4681 gaacccgctg tgctactgcg agctgttttt gctattgccc gcgctgctgt acaacctgtg
     4741 gctgccgctg cgcacctgct accaaatcta cctgtttgct gtgacgctgg ccacctgcgc
     4801 cattgcctat tacagctttg ccaaaatcgc ggccagccgc cgcctgggcc tgctgggggc
     4861 gctgctttac accctgtcct gctaccggct cgtctgcgtc tacacccgcg ccgctgcggg
     4921 cgaattcacg gccatggcct tcttcccgct ggtgctggcg gccagacgat tgcggagccg
     4981 ctgttcgaat tcggccattt tct
//
LOCUS       DBMDSQ010000136         4987 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_4071_length_4987_cov_1.799836, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000136 DBMDSQ010000000
VERSION     DBMDSQ010000136.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 4987)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4987)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4987
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4071_length_4987_cov_1.799836"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..662)
                     /locus_tag="ACZPAX_06010"
     CDS             complement(<1..662)
                     /locus_tag="ACZPAX_06010"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR1113486.1"
                     /translation="MSKKEPKVAIVHDWLVGYAGGDRVVDAMKHVFPNAVIYTLVYDP
                     NNMPEWFKSYDIRTSWFQKVPFSNKLYKAMLPLMPAAFEAFDLTEYDLVLSSCSSCSK
                     GVITRPDAVHICYCHTPIRYVWDFYYTYRANANWLARLVMPGQMHKMRVWDKCAADRV
                     DYFIANSHYISQRIKKYYRRDSDVIYPGCHINEAPFVEKEDFYLTVGRLTWYKRVDLA
                     VQA"
     gene            complement(696..2108)
                     /locus_tag="ACZPAX_06015"
     CDS             complement(696..2108)
                     /locus_tag="ACZPAX_06015"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR03023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HIR1113487.1"
                     /translation="MLEKNQRFKSLLFVLLDAVLLFVGFLLANYIRFNYVRYFEPGGA
                     GPALAVAQDPRNIIAGLATSLVLVLIYWVAGIYNNARLHGLAERCTKIAVINAAGLLI
                     FIGALYLLHLDDYSRIVLALFYILSTAAVVFERMVLRWVDRARRRKGLGLRHILLVGG
                     GKSAALYLRALEHNPYYGFVVDGYLAKVEEPGLGVKFLGGYDNLAAKLDEIAVDEVVV
                     ALEADEIELLPHVFAACDKNGVRITMVPFYSDYLPARPTIDVLDECKLINVRQTPFDN
                     LLNAAVKRGLDILGSLILIILTSPIMLVTAIGVKLSSPGPIIFKQERIGLNKKPFMMY
                     KFRSMRVNVQQETGWSTDYDPRKTHFGSFIRKFSLDELPQFFNVLKGDMSLVGPRPEV
                     PFHVEHFKEEIPRYLVRQQVRPGCTGWAQVHGLRGDTDIAERIRYDIWYIENWTVALD
                     IKIIFRTVFGGKMVNDEKLQ"
     gene            complement(2176..3171)
                     /locus_tag="ACZPAX_06020"
     CDS             complement(2176..3171)
                     /locus_tag="ACZPAX_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005943523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR1113488.1"
                     /translation="MPRKEGQKRKLLVLLEILARETDEKHPLSVPQLVEELQEHGVVA
                     ERKSVYDDLNTLNNLPDSKYEIVQQRGRGGGYYMTETPFELAELKLLVDAVYASKFIT
                     ARKSKILIDKLGQFTSKYRQAELDRKVLVSGRIKSTDEKILYTVDALHTAITAGQQVQ
                     FKYCDWNLQKRMTPRHDGQLYRVSPWVLVWENGNYYLIAYTEGRLKHYRVDKMQNVHQ
                     LPDTTREGAEEYANFDVNAYMQQMFGMFNGPLKRVTLLCENRFAGAMIDRFGTGPILT
                     PCPDGEHFTMTAEIQVSPQFFGWVAGFGTGVVVSGPPEVRAEMKKTLDQLQELYR"
     gene            complement(3255..4142)
                     /locus_tag="ACZPAX_06025"
     CDS             complement(3255..4142)
                     /locus_tag="ACZPAX_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015564704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HIR1113489.1"
                     /translation="MQQEITRELVQSWLPRRQADSNKGSYGSVLAAAGSLAYRGAAAL
                     CAEGALRGGAGLVYLASVEPVVQMVLARTPECCACGCRTAAGGGIHPQDAAALRSWFA
                     EKHAVLLAGPGLGEGAGEVCRALLGKNPPWAGAVLDADALNSLAAGRLRDPLPENTIL
                     TPHPGEAARLLGCTVADVQADREQAVLRLAETYHCVAVLKGSGTLIAAPGGELLHNAA
                     GNAGLARGGSGDILAGLTAGLLAAGLKQNRTPAQMAACAVWLHGTAADRCARRRSMTA
                     MLPTDIFADLGEILAEMEL"
     gene            4219..4956
                     /locus_tag="ACZPAX_06030"
     CDS             4219..4956
                     /locus_tag="ACZPAX_06030"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005922053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein lysine
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HIR1113490.1"
                     /translation="MIRALEEILAETKNMVFFGGAGVSTESGIPDFRSVDGLYHQKFK
                     YPPETMLSHSFYERHTDEFYDFYRQKLIVHGAKPNAAHLRLAALEREGKCKAVVTQNI
                     DGLHQAAGSKVVYELHGSTLRNYCTHCGKFFPVSFIEDAAGVGDGVPRCDECGGIVKP
                     DVVLYEEGLDEQTMENAVNAIRKADTLIVGGTSLAVYPAAGLLRYFRGDNLVVINKQP
                     TSADAMANLLIHEPIGRTLDPNDNVEA"
BASE COUNT         1016 a         1479 c         1505 g          987 t
ORIGIN      
        1 gcctgcacgg cgagatcaac gcgcttgtac caggtcaggc ggcccacggt gaggtagaaa
       61 tcctcttttt cgacaaaagg tgcttcgttg atgtggcagc cggggtagat cacgtcactg
      121 tcacgccggt aatatttttt gatgcgctgc gagatataat gcgagttggc gataaaataa
      181 tctacgcggt cggcggcgca cttgtcccag acgcgcatct tgtgcatctg tcccggcatg
      241 acgaggcggg caagccagtt ggcattggcg cggtaggtgt agtaaaaatc ccacacatag
      301 cggatgggcg tatggcagta gcagatatgc acggcatccg gccgggtgat aacacccttg
      361 gagcaggacg agcaggacga cagcacaagg tcgtactcgg tcagatcgaa cgcttcaaac
      421 gcggcgggca tcaatggcag catggccttg tagagcttgt tggaaaacgg caccttctgg
      481 aaccagctgg tgcggatatc gtagctcttg aaccattcgg gcatattgtt ggggtcgtac
      541 acaagggtgt agatgacagc attggggaaa acgtgcttca tagcgtccac aacgcggtcg
      601 ccgccggcat agccgacgag ccagtcgtgg acgatggcaa ctttcggttc ctttttgctc
      661 atgacggaca ctcctgttcg gtaacgcggg gcgcgttact gcaatttttc gtcgttgacc
      721 atcttgccgc cgaacaccgt gcggaagatg atcttgatat ccagcgccac ggtccagttt
      781 tcgatgtacc agatatcgta gcggatgcgc tcggcaatgt cggtgtcgcc gcgcagaccg
      841 tgaacctgtg cccagcctgt gcagccgggg cgtacctgtt ggcgcacgag atagcgcggg
      901 atttcctctt tgaagtgctc gacgtggaag ggcacctctg gccgcgggcc gacgagggac
      961 atatccccct tcagcacatt gaagaactgg ggcagctcgt cgaggctgaa cttgcggata
     1021 aagctgccga agtgggtctt gcgggggtcg tagtcggtgg accagcctgt ctcctgctgc
     1081 acattgaccc gcatggagcg gaacttgtac atcatgaagg gctttttgtt taggccgatg
     1141 cgctcctgtt taaagatgat cggcccgggg ctggacagct tgacgccgat ggccgtgacc
     1201 agcatgatgg ggctggtcag gataatcagg atcaggctgc ctaggatatc cagcccgcgc
     1261 ttgacggcgg cgttgagcag gttgtcaaag ggcgtctgcc gcacgttgat gagtttgcac
     1321 tcgtccagca catcgatggt cggccgcgcg gggaggtagt cgctgtaaaa cggcaccatc
     1381 gtaatgcgca cgccgttttt gtcgcaggcg gcaaaaacat ggggcaataa ttcgatttcg
     1441 tcggcttcca gcgccacgac gacctcatcc acggcgattt cgtccagctt ggcggccagg
     1501 ttgtcatacc cgcccaggaa ctttacgccc aggccgggct cttccacttt ggcaagatag
     1561 ccgtccacga cgaagccgta gtaggggtta tgctccagcg cgcgcagata aagcgcagcc
     1621 gatttgccgc cgcccaccag caggatatgg cgcagaccca gccccttgcg gcggcgggcg
     1681 cggtcgaccc agcgcaggac catccgctca aacacgacgg cggcggtgga gagaatgtag
     1741 aacagcgcca gcacaatgcg ggagtagtcg tccaagtgca gtaaatacag cgcaccaatg
     1801 aaaatcaaga ggcccgctgc gttgataacg gcaattttgg tgcagcgctc ggccaggccg
     1861 tgcagccgcg cattattgta aatgcccgcc acccagtaga tgagcaccag caccagcgac
     1921 gtggccagcc cggcaatgat gttgcgggga tcctgcgcca cggccagggc cgggccggca
     1981 ccgccgggct caaaatagcg gacataatta aaacgaatat agttggcaag cagaaagccc
     2041 acaaacagca gcactgcatc cagcagcaca aacagcaggc ttttaaagcg ttggtttttt
     2101 tccagcaagg gtagcctccg tgaaacaagt agaagtaaac aatctattct accatatttc
     2161 tactgttatt gcaaatcagc ggtacaattc ctgcagctga tccaaggtct ttttcatctc
     2221 ggcgcggacc tccggcggac cggagacgac gacgccggtg ccaaaaccgg ccacccagcc
     2281 gaaaaactgc gggctgacct gaatctcggc ggtcattgtg aaatgctcgc cgtcggggca
     2341 gggcgttaag atcggcccgg tgccgaaccg atcgatcatc gccccggcaa agcggttctc
     2401 gcacaggagc gtcacccgct tcagggggcc gttgaacatg ccgaacatct gctgcatata
     2461 agcattcacg tcaaaattcg cgtattcctc ggcaccttcg cgggtggtgt cgggcagctg
     2521 gtggacgttc tgcatcttgt ccacgcgata gtgcttgagc cgcccctcgg tgtaggcgat
     2581 gaggtaatag ttgccgttct cccaaaccag cacccagggg ctgacacggt agagctggcc
     2641 gtcgtggcgc ggggtcatcc gcttttgcag gttccagtcg cagtatttga actgtacctg
     2701 ctgccctgcc gtgatggcag tgtgcagtgc atcgacggtg taaaggattt tctcgtcggt
     2761 gctcttgatg cggccgctga ccagaacctt gcggtccagc tccgcctgcc ggtatttcga
     2821 ggtgaactgc cccagcttgt caatgaggat tttggatttg cgcgccgtga taaatttgct
     2881 ggcgtagaca gcgtccacca gaagtttcaa ctcggccagc tcaaagggag tctcggtcat
     2941 atagtagccg ccgccgcggc cgcgctgctg cacgatttcg tacttggaat cgggcaggtt
     3001 gttcagcgtg ttcaggtcat cgtagacgct cttgcgctcg gccacgacgc catgctcctg
     3061 caattcttcc accagctgcg gcacgctgag gggatgcttt tcgtccgtct cgcgggcgag
     3121 aatctccaaa aggacaagca gcttgcgttt ttggccttct ttgcgcggca taacaacggc
     3181 tcctttccgg caatatgcag atatagtcct attatacacc ctataaaggg agagggcaag
     3241 agggacgcct ccccttacaa ctccatctcc gccaaaatct cccccaaatc tgcgaaaata
     3301 tccgtgggca gcatggcggt catgctgcgg cggcgggcac agcggtcggc ggcagtgccg
     3361 tgcagccaga cggcgcaggc ggccatttgg gcgggggtgc ggttctgctt cagcccggcg
     3421 gccagcagcc cggcagtcag cccggccaga atatccccgc tgccgccccg ggccagcccg
     3481 gcgttgccgg cggcgttatg caaaagctcc ccgcccggcg cggcaatcaa cgtgccgctg
     3541 ccctttagaa cggcgacgca gtgataggtt tctgccagcc gcaaaacagc ctgctcccgg
     3601 tcggcttgta catcggccac ggtgcagccc agcagtcgcg cggcctcgcc ggggtggggc
     3661 gtcaggatgg tattttccgg caaggggtcc cgcagcctgc ccgccgcaag cgagttcagt
     3721 gcgtcggcgt ccagcaccgc acccgcccac ggcggattct tgcccagcag ggcgcggcag
     3781 acttcccctg ccccctcccc caagcccggc ccggccagca gaacggcgtg tttttcggca
     3841 aaccagctgc gcagtgcggc ggcgtcctgg gggtggatgc ccccacccgc cgccgtgcgg
     3901 cagccgcagg cacagcattc cggcgtgcgg gccagcacca tctgcacgac cggttccaca
     3961 ctggccaggt acaccagccc cgcgccgccg cgcaatgcgc cctcggcaca cagggccgct
     4021 gcgccgcggt aggcaaggct ccccgctgcg gccagtacac tgccgtagct gcccttgttg
     4081 ctgtccgcct gccgccgggg cagccagctt tgcaccagtt cccgcgtgat ttcctgctgc
     4141 atcttgcatc cctccgagaa tgtattataa taagtatatt ataaagcatt tgcagcgaaa
     4201 acgaaagggg cagtttgcat gatccgcgca ttggaagaaa ttttggcaga gacaaaaaac
     4261 atggtctttt tcggtggggc gggcgtctcg accgagagcg gcatcccgga tttccgctcg
     4321 gtggacgggc tgtaccatca aaagttcaaa tacccgccgg agacgatgct ctctcacagc
     4381 ttttacgaac gacacaccga tgaattttac gacttctacc gccagaagct catcgtacac
     4441 ggcgcaaagc ccaacgcggc ccacctgcgg ttggcggcgc tggagcgcga gggcaagtgc
     4501 aaggccgttg tgacccagaa cattgacggc ctgcaccagg cggcgggcag taaggtcgtg
     4561 tatgagctgc acggctctac cttgcgcaac tactgtacgc actgcggcaa atttttcccg
     4621 gtcagcttta ttgaggatgc ggccggcgtc ggcgacggcg taccccgctg cgacgagtgc
     4681 ggcggcatcg tgaagcccga cgttgtgctc tacgaggaag gcctggacga gcagacgatg
     4741 gaaaacgccg tgaacgccat tcgcaaggcc gacaccctta ttgtcggcgg cacaagtctg
     4801 gcggtctacc cggcggcggg gctgctgcgg tatttccggg gtgacaacct tgtcgtcatc
     4861 aacaaacagc ccacctcggc ggacgccatg gccaatctgc ttatccacga gccaatcggc
     4921 cgcacgctgg accccaatga caatgtggag gcgtaatttt tacacaaaaa tctatggttt
     4981 tttcaaa
//
LOCUS       DBMDSQ010000137         4972 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_4091_length_4972_cov_2.042086, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000137 DBMDSQ010000000
VERSION     DBMDSQ010000137.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 4972)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4972)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4972
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4091_length_4972_cov_2.042086"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(740..2230)
                     /locus_tag="ACZPAX_06035"
     CDS             complement(740..2230)
                     /locus_tag="ACZPAX_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005934483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HIR1113491.1"
                     /translation="MKIGFDNDKYLAMQSAHIRERIAQFDNKLYLEFGGKLFDDYHAS
                     RVLPGFQPDSKLQMLLQLKEQAEVVVVISAEDIVANKMRGDYGITYDVDVLRLIDAFQ
                     GVGLFVGSVCVTKYTDAPEVTAFEQKLNALGIRTFRHYKIPGYPNDVAHIVSDDGYGK
                     NDYIETERPLVVITAPGPGSGKMAVCLSQLYHEYKRGVKAGYAKFETFPIWNLPLKHP
                     VNLAYEAATADLNDVNMIDPFHLEAYGKTTVNYNRDVEIFPVVNAMFELIAGKSPYRS
                     PTDMGVNMAGNCIINDEVCREASLNEIMRRYFKCLCDQKASGIVKQERFKLELLMNQA
                     GIALGQREVEKRAHATSETTDGKPAAAIELADGTIVTGKTGPLLGAASSALLNALKKL
                     AGIDQEIDLVSARAIAPIQMLKTNYLGSKNPRLHTDEILIALSSSVSENEYAAKAMEQ
                     IPNLKGCDIHSTVILSSVDADTLKKLGMYLTCEPTYEEDDRMYHKK"
     gene            2299..4383
                     /locus_tag="ACZPAX_06040"
     CDS             2299..4383
                     /locus_tag="ACZPAX_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009324742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HIR1113492.1"
                     /translation="MPFCGGTLSWPEEALRANIIAQVSLALNRIWTEWYPSRGYSFNI
                     TGSPGYDQAYVNGRTVFAVMERLTAELFNTFVQRSGDAEPYFTEYCDGKTVTCPGMKQ
                     WGTVDRAREGLNALQILRYYYGNRVQLVTTNNIAAIPSSYPGSPLQRGSTGTNVRILQ
                     KQLSRIAKDYPSFGKPAVTGTFDEATENSVKRFQKQFGLTADGIVGKATWYKISYIYV
                     SVKDLAELTSEGETAEGIQSAGGWPGTVLRRGSTGSSVEQVQFWLSDLAQFDSSLVRV
                     SVDGNYGAATERAVRAFQQKQNLTADGVVGQTTWNALYAAWVDAQSDLGGTAWPGTAL
                     RRGDTGMEVRLVQFWLRLAADNYSALHTVTVDGSYGAATLSAVTAFQSLFGLTADGVV
                     GRSTWNKLKEVGLAVANKIVAANVAPGQFTATTREGSSGTAVRAVQYYLRRLAAYYSD
                     VPTVTVDGKFGAATTRAVKAWQTRAGLTVDGVVGRLTFQSLYDAALALDTSGPVVRTV
                     SLPAPDAALRPGDTGAEVLRLNRMLLFLSQWISEINFTAGGTPGSTFDPELEIAVRSA
                     QRYFGLDETGVVTADDWAVFRQAALDLLAASPAGASPKPGGVWPAGALALGSSGPAVL
                     QVQRWLNVVASVDQTADFVPESGQFDAATQAALESYQLTAGLEPLGVVDADTWESLRL
                     TAQGLCRECQEG"
     gene            4387..>4972
                     /locus_tag="ACZPAX_06045"
     CDS             4387..>4972
                     /locus_tag="ACZPAX_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002610643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HIR1113493.1"
                     /translation="MPKVYVYDSYDNKFFRYNLSENDPMPYSADTTLRVREFRGSSKA
                     NVLWTTTAAMEAWNLTRRTYGSGIPVGYAFKRIWEGGHGTTSQHYAGVAFDVGQSTTA
                     ANRRKIYKAAARTGAWGYVEPLSMTPTWVHFDRRYGKPACRSTTAGYPTLRRGSRSTY
                     VLILQDALNALGYSTNTLDGIFGKATENALKACQR"
BASE COUNT          988 a         1368 c         1596 g         1020 t
ORIGIN      
        1 aggatcaaac tctttataaa tggtatttaa tcacttacaa agtgttaaat cgctgtaatc
       61 actcagacac aatcgcttgc gtcctgtatg aattacttgt aaattttgga attgattatc
      121 gtgggtttcc aaacccacgt tcaaggttcc acaagtttca gttttgttca attttcaagg
      181 tcctgctgca ctgtcgcgtc tccgtgacag cttgtatatt atatatcaga aacgcttgat
      241 tgtcaagggg ttttctgaaa gttttttgaa gaatttcttc ttcaaaaaac atatacaggc
      301 tgttatgaag cgcttttcgc ggcgtgtccg ccgcgagaca gcttgattat tatagcaagg
      361 agtatgactc ttgtcaacag tttttttggt ttttttgaca aattttgaga tcaatttttg
      421 ggcaaggtgt atattggggt ggtgggtggt gataggtaca aggggttgcg atgggaaggt
      481 ggggcgggcg gcatgtatgc cgcccctaca tgggcgacct ggcgggagta aggggcaata
      541 aaggcaatgt gcggcggagg gtcaagcccc ctccctacaa tatggaagat taaattgcag
      601 taacggcaag gtggagggcc gggcatgccc ggtcactacc ggggttgcga aagattatgt
      661 ggcaataagg ggatggccgc tgagagggat aaatttcttc tctactgcaa aagggagagg
      721 ttgtccctct cccttttgct tactttttat ggtacatacg gtcgtcttct tcgtaggtgg
      781 gttcgcaggt caggtacatg ccgagctttt tcagggtatc ggcgtcaacg ctggacagga
      841 tgaccgtgga atggatatcg cagcctttca agttgggaat ctgctccatg gccttggcgg
      901 cgtactcatt ctcagaaacc gagctggaca gggcaatcaa aatctcgtcg gtgtgcaggc
      961 gtgggttctt gctgcccagg tagttcgttt tcagcatctg gatcggcgcg atagcgcggg
     1021 cggacaccaa atcgatctcc tggtcgatgc ctgccaattt cttgagggcg ttcagcaatg
     1081 cagaggatgc cgcgcccagc agcgggccgg tcttgccggt gacgatggtg ccgtcggcca
     1141 gttcaatggc agcggcgggt ttgccgtcgg tagtctccga cgtggcgtgg gcgcgttttt
     1201 cgacctcgcg ctggcccagc gcaatgcctg cctggttcat cagcagttcc agcttaaaac
     1261 gctcctgttt gacaatgccg ctggccttct ggtcacagag gcacttgaaa taccgacgca
     1321 tgatctcatt cagcgaggct tcgcggcaga cctcatcgtt gatgatgcag ttgcccgcca
     1381 tgttgacgcc catatccgtg ggtgaacggt aggggctctt gcccgcaatc agttcaaaca
     1441 tggcgttgac gaccggaaaa atctcgacat cgcggttgta gttgactgtg gtcttgccgt
     1501 aggcttccag gtgaaagggg tcgatcatat tgacgtcgtt cagatcggcg gtagcggcct
     1561 cgtaggccag gttgaccggg tgcttcagcg gcaggttcca gatgggaaac gtctcgaact
     1621 tggcatagcc cgccttgacg ccgcgcttgt actcatggta gagctgggac aggcagaccg
     1681 ccattttgcc gctgccgggg ccgggggccg tgatgacgac gagcgggcgc tcggtctcga
     1741 tgtaatcatt cttaccgtag ccgtcgtcgc taacgatatg ggccacatcg ttggggtagc
     1801 cgggaatctt gtaatggcgg aaggtgcgga tgcccagtgc gttgagcttc tgttcaaagg
     1861 ctgtgacttc aggcgcgtcg gtgtactttg tcacgcagac gctgcccaca aacagaccca
     1921 cgccctggaa cgcgtcgatg agacgcagca cgtccacatc gtaggtaatg ccgtagtcac
     1981 cacgcatctt gttggcgacg atatcctcgg cgctgatgac gacgacgacc tcggcctgct
     2041 ccttcagctg caaaagcatc tgcagcttgg aatccggctg gaagcccggc agcacgcggg
     2101 aggcatggta gtcgtcgaaa agcttaccgc caaattccaa atagagttta ttgtcaaact
     2161 gggcaatgcg ctcccggata tgggcagact gcatcgcaag gtatttgtca ttatcgaatc
     2221 ctattttcat ctgcgttccc cttttccctg tctttaaacc atatgaatta agtatagcat
     2281 ttttcgcggg ccgttgcaat gccattttgt ggcggtacgc tttcctggcc ggaagaggcc
     2341 ctgcgcgcca acatcattgc ccaggtcagc ctggcgctga accgcatctg gaccgagtgg
     2401 taccccagcc ggggatatag tttcaacatc acaggctcac cagggtacga ccaggcgtat
     2461 gtcaacggcc gcacggtgtt tgcggtcatg gagcggctga ccgccgagtt gttcaacacc
     2521 tttgtgcagc gcagcggcga cgccgaaccg tactttaccg agtactgcga cggcaagact
     2581 gtgacctgcc cgggcatgaa gcagtggggc acggttgacc gtgcacggga ggggctgaac
     2641 gcgctgcaaa tcctgcgcta ctattatggg aaccgcgtgc agctggtgac gaccaacaac
     2701 attgcggcca ttccgtccag ctacccgggc agccccttac agcgcggaag caccgggacg
     2761 aatgtgcgca tcctgcaaaa gcagctgtcc cgcatcgcga aggactaccc atctttcggc
     2821 aagcccgccg tgaccgggac gttcgacgag gcgaccgaga acagcgtgaa aagatttcag
     2881 aagcagttcg gcctgacggc ggacggcatt gtgggcaagg cgacgtggta caaaatcagc
     2941 tatatctacg tcagcgtcaa ggacctggcc gaactgacca gcgagggcga gaccgccgag
     3001 ggcatccaaa gcgcgggcgg ctggccgggg accgtactgc ggcgcggcag caccggcagc
     3061 agcgtggagc aggtgcagtt ctggctttcc gacctggcgc agttcgattc gtcgctggta
     3121 cgcgtgagcg tggatggcaa ctacggcgca gcgactgagc gggccgtgcg ggcgttccag
     3181 caaaagcaga acctgacggc ggacggtgtc gtcgggcaga cgacctggaa cgcgctgtac
     3241 gctgcgtggg tggacgcgca gagcgatttg ggcggcacag cctggcccgg gacggcactg
     3301 cggcgcgggg ataccggcat ggaggtacgg ctcgtgcagt tctggctgcg gctggcagcg
     3361 gacaattaca gtgccctgca caccgtgacc gtggacggca gctacggcgc agcaacgctc
     3421 agtgcggtga cggcgttcca gagtttgttc gggctgacgg ctgatggcgt cgtcgggcgc
     3481 agcacctgga ataagctgaa agaggtcggc ctggcggtgg caaacaaaat cgtggcagcc
     3541 aatgtcgcac ccgggcagtt cacggccacg acgcgggagg gcagcagcgg gaccgcggtg
     3601 cgggctgtgc agtattacct gcggcggctg gcagcctatt acagcgatgt gcccaccgtg
     3661 actgtggatg gaaagttcgg cgcagcgacg acccgggctg tcaaggcgtg gcagacccgc
     3721 gccgggctga ccgtggacgg cgtggtgggg cggctgacct tccaaagcct gtatgacgcc
     3781 gcactggcat tggacaccag cgggccggtg gtgcgcacgg tcagcctgcc cgcacccgat
     3841 gcggcactgc gccccggcga tacaggcgcg gaggtgctgc ggctgaaccg gatgctgctg
     3901 ttcttgagcc agtggatatc ggagatcaac tttacggccg gcggcacacc gggcagtacc
     3961 tttgaccccg agctggaaat cgccgtgcgc agtgcgcagc ggtactttgg gctggacgag
     4021 accggcgttg tcaccgccga cgattgggct gtgttccgcc aggcagcgct tgacctgctg
     4081 gccgccagcc ccgccggggc cagcccgaaa ccgggcggcg tatggcccgc cggggcgctg
     4141 gcgctgggca gctccggtcc tgccgtttta caggtgcagc ggtggctcaa tgtagtggcc
     4201 tccgtcgacc agaccgccga ttttgtaccc gagagcggcc agtttgacgc cgcgacccag
     4261 gccgcgctgg aaagctacca gctgactgcc ggactggagc cgctgggcgt tgtggacgcc
     4321 gacacctggg aaagcctgcg cctgaccgcg cagggacttt gccgcgaatg tcaggaggga
     4381 tagggtatgc caaaagtcta tgtgtacgat tcctacgaca acaagttttt tcgctacaat
     4441 ttgagcgaga acgacccgat gccgtacagc gccgacacga cactgcgggt tcgggagttc
     4501 cgcgggtcca gcaaagccaa cgtgctgtgg acgacgaccg ccgccatgga ggcgtggaat
     4561 ctgacccgcc gcacctacgg cagcggcatc ccggtggggt acgctttcaa gcgcatctgg
     4621 gagggtggcc acggcacgac gagccagcac tacgcaggcg tggcctttga cgtggggcag
     4681 agcaccaccg ctgcgaaccg ccgcaagatt tacaaggccg ccgcgcggac cggggcgtgg
     4741 gggtatgtgg agccactttc gatgacgccg acctgggtcc attttgacag gcggtacggc
     4801 aagcctgcgt gccgcagcac cacagcgggc taccccaccc tgcggcgcgg cagccgcagc
     4861 acgtatgtgt tgatcctgca ggacgcgctg aacgcgctgg ggtattccac caacacactg
     4921 gacggcatct tcggaaaggc gaccgagaac gcgctgaaag cctgccagcg gc
//
LOCUS       DBMDSQ010000138         4961 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_4100_length_4961_cov_1.700000, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000138 DBMDSQ010000000
VERSION     DBMDSQ010000138.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 4961)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4961)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4961
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4100_length_4961_cov_1.700000"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(311..469)
                     /locus_tag="ACZPAX_06050"
     CDS             complement(311..469)
                     /locus_tag="ACZPAX_06050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113494.1"
                     /translation="MDTKATIYAAPKELTDRQLVNTPIGSVCMSRTEYELYRAELELR
                     KAKETQHL"
     gene            complement(485..1861)
                     /locus_tag="ACZPAX_06055"
     CDS             complement(485..1861)
                     /locus_tag="ACZPAX_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005936940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1113495.1"
                     /translation="MSTPAKSKSMTEGPLAKQILLVSLPLALSNLLQILFNLSDVAVV
                     GRFAGSTALGAVGSTATFVTLFTGFLIGLSNGINVLVARFYGARHPKDVSKTVHSALL
                     VSLAAGVLLLFIGLVGSPALLRLLNTKEDLLPGAILYLRVYFLGMPALALYNFGNAVF
                     SAIGDTKKPLTYLSIAGALNIVLNLFFVIVCKLDVAGVALASTIAQCVSAGLILHALT
                     KVRDCYALDFHKIKLDIPTTKRILLLGIPAGFQNAIFAIANLFIQAGVNSFDSLMVKG
                     NSAAANADALIYDAMAAFYMACASFMSQNYGAGKLDRVKKSYFIALAYSFGLGAVLGG
                     GLLVFGRQFLALFTTESAVIDAGMKRILVMGWAYCTSAFMDCTIAASRGLGRTIGPTV
                     IVILGSCVFRVAWVYTIFAHFHTIPSLYLLYPCSWALTAAAEIAYFVFAYRQSVQRLQ
                     QHDALARL"
     gene            complement(2046..2594)
                     /gene="nrdG"
                     /locus_tag="ACZPAX_06060"
     CDS             complement(2046..2594)
                     /gene="nrdG"
                     /locus_tag="ACZPAX_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HIR1113496.1"
                     /translation="MNYATIKYCDIANGEGVRTSLFVSGCRRHCPNCFNAVAWDFNYG
                     APFTDAVKEQIIESLAPGYINGLSLLGGEPLEPENQRALLPFIKEVKVRCPGKTIWCY
                     TGNNLEHEVWQPGCGRCEVTDEFLQYIDVLVDGEFVQDKYDISLRFRGSSNQRIIDMN
                     KTRAAGKIVLWQDDPIFADHKM"
     gene            3016..>4961
                     /gene="nrdD"
                     /locus_tag="ACZPAX_06065"
     CDS             3016..>4961
                     /gene="nrdD"
                     /locus_tag="ACZPAX_06065"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002565424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HIR1113497.1"
                     /translation="MKIIKRNGSEAVFDITKIISAITKANKVVPEAQRLTKQQIIEIS
                     DHVQEVCYARGHAMNVEEIQDIVEDAIMATGAYEVARKYITYRYVQSLKRTHNTTDDK
                     ILSLIECNNEEVKQENSNKNPTVNSVQRDYMAGEVSKDLTMRMLLPPEIVKAHEEGII
                     HFHDADYYAQHMHNCDLVNLDDMLQNGTVISGTLIEKPHSFSTACNIATQIIAQVASS
                     QYGGQSISLTHLAPFVDVSRKKIRRDVEAEMKDLGIDPGEEKISEIVEKRLREEIKRG
                     VQTIQYQVVTLMTTNGQAPFITVFMYLNEAGDNQRLKSDLAIVVEEMLRQRYQGVKNE
                     AGVWITPAFPKLIYVLEDDNIYEGQPYYYLTKLAAKCTAKRMVPDYISEKKMLEYKVD
                     ANGEGHCFTCMGCRSFLTPYIDENGKPKYYGRFNQGVVTINLVDVALSSGGDFDKFWQ
                     LFDERLELCHKALMCRHNRLKGTLSDAAPILWQYGALARLKKGEPIDKLLYGGYSTIS
                     LGYAGLYECCKYMTGKSHTDPAAKPFALNVMQHMNDACAKWKNQHNIDFSLYGTPLES
                     TTYKFAKCLQKRFGVVPGITDRNYITNSYHVHVTEHIDAFTKLKFESEFQKLSPGGAI
                     SYVEVPNMQDNLEAVIKVMQFIYE"
BASE COUNT         1143 a         1445 c         1397 g          976 t
ORIGIN      
        1 cataaccgcc gagcgtgccc cgatgatccg tgcgacacct acaaggatgc ttccgagtgg
       61 gagaccaagg ctctggatct ggctggccgc ttcatcaaga acttcgccaa gtacgaaggc
      121 aacgagcacg gcaaggctct ggtctctgcc ggcccccagc tgtaatttga gcgactgtgc
      181 ttaaaatcat agcataacac cccgcccgca cgtcacggtt ttcgtgatgt gtgggcgggg
      241 tgcttctata cactgaaaaa caaggccccc gggcgtgctg gcaggcagca gcgcccgggg
      301 gcctgacgtg ttataaatgc tgcgtttcct tggctttgcg cagttccagt tctgcccggt
      361 acagctcgta ctcggtgcgg ctcatgcaga ccgagccgat gggcgtgttg acgagttggc
      421 ggtcggtcag ttcttttggc gcggcgtaaa tggtggcttt ggtgtccata cctctcccct
      481 ccccttacag gcgcgccagg gcgtcgtgct gctgcaagcg ctggacggac tggcggtagg
      541 caaagacaaa ataggcgatc tctgcggcgg cagtcagcgc ccaggagcag ggatacagca
      601 ggtacagcga gggaatcgtg tggaagtggg cgaagatcgt gtagacccac gccacgcgga
      661 atacgcagga ccccagaata acgataacgg tggggccgat ggtccggccc agcccgcggg
      721 aggcggcaat cgtgcagtcc atgaaagccg aggtgcagta ggcccagccc atgactagga
      781 tgcgcttcat gcctgcgtcg atgacggcgc tctcggtggt gaacagcgca aggaactggc
      841 ggccgaacac cagcagccca ccgcccagca ccgcgcccag cccaaaggag taggccagcg
      901 cgatgaagta gcttttcttt acgcggtcca gcttgcccgc accgtagttc tggctcataa
      961 agctggcgca ggccatgtaa aacgccgcca tagcgtcata aatcagggca tccgcgttgg
     1021 cggcggccga gttgcccttg accatcagcg agtcgaagga gttgacgccc gcctggataa
     1081 acaggttagc gatagcgaaa atcgcgttct ggaagcctgc cggaatcccc agcagcaaaa
     1141 tacgttttgt cgttggaatg tctaatttta tcttgtgaaa gtccagcgca tagcagtccc
     1201 ggacttttgt cagcgcgtgc aggatcagcc ccgccgagac gcactgggca atcgtgctgg
     1261 ccagcgccac acccgcgaca tcgagcttgc agacgatgac gaagaacagg ttcagcacaa
     1321 tattcagcgc acctgcgata gaaagatagg tcagtggctt cttggtatcg ccgatggcgc
     1381 tgaacacagc gttgccgaag ttatagagcg ccagcgcggg catacccaaa aagtagacgc
     1441 gcaggtacaa gatggcaccg gggagcaggt cctctttggt attcagcagc cgcagcagcg
     1501 cgggcgagcc gaccagaccg ataaacagca gcagcacacc cgccgccagg ctgaccagca
     1561 gcgccgaatg gacggtcttg ctgacgtcct tggggtgcct ggcaccgtaa aaccgcgcca
     1621 ccagcacatt gataccgttg ctgaggccga taagaaagcc ggtgaacagt gtgacgaacg
     1681 ttgccgtgga gccgactgcg cccagcgccg tggagcctgc aaagcggccc acaacggcaa
     1741 catcggacag gttgaacaga atttgtaaaa ggttagacag ggccagcggc agactgacca
     1801 gcagaatttg tttggccaat ggcccttcgg tcatggattt ggatttagcg ggggtggaca
     1861 tagtagatga tgattgctcc tatttttgaa atatggcaga ggtgtagggg ccgggcatac
     1921 ccggccctcg cgtggaaggc agacggcttc ttaccgcagg gctgcgggcc gcatatctgc
     1981 ggcccctgca cctcggcctt gcggttcgca ggagcggact ccatatccgc ccgcggccgc
     2041 ctccgttaca tcttgtgatc cgcaaaaatc gggtcgtcct gccaaaggac gatctttccc
     2101 gccgcgcggg tcttgttcat gtcaatgatg cgctggttgc tcgaaccgcg gaaccgcagg
     2161 ctgatatcat acttgtcctg cacaaattcg ccgtccacca gcacatctat atattgtagg
     2221 aactcgtccg tgacctcgca gcggccgcag ccgggctgcc agacctcgtg ttcgagattg
     2281 ttgccggtgt agcaccagat ggtcttaccc gggcagcgga cctttacctc tttgataaag
     2341 ggcagcagcg cacgctggtt ttccggctcc agcggctcgc cgcccagcag cgacagaccg
     2401 ttgatgtagc cgggggccag gctctcgatg atttgctcct tcaccgcgtc ggtaaagggc
     2461 gcgccatagt tgaaatccca cgccactgcg ttgaaacagt tgggacaatg gcggcggcag
     2521 cccgagacaa acaggctggt gcgcacccct tccccgttgg caatatcaca atatttgatg
     2581 gtggcgtagt tcatggcagt tctcctgtgg ggcatcccca tatcttgtgt aagggcttgg
     2641 ggcatacccc aaaaccttac ctagtatacc agctttaccg acataatgca accgtgaaaa
     2701 caaaatacag cggtgcgttt tctgcgatac agcgtcattt tgtgtagaat cgctcaaaat
     2761 cactaaaaat tctgcctcat ttttggcagt atcctttttg cacgttgagg cgacgaaaac
     2821 ggaatatttt gttctttgaa tatcatataa cacaatatct agtggtttca tccattgact
     2881 tttccctctg attgcggtat tatagtcttg ctcgcgaaac gggctttgta caacccggca
     2941 acagatacgt gttttataca gtgcgtattg ccatgttccg catctgttta ttctattata
     3001 aaaagaggag tcaccatgaa gatcatcaag cgcaacggca gcgaagctgt ctttgacatc
     3061 accaaaatca tttcggccat cacgaaggcg aataaggtcg tgcccgaggc ccagcgcttg
     3121 acgaagcagc agatcatcga gatcagcgac catgtgcagg aggtctgcta tgcccgcggc
     3181 cacgccatga acgtggaaga gattcaggat atcgtcgagg atgccatcat ggccaccggc
     3241 gcgtatgagg tggcccgtaa gtacatcacc taccgctatg tgcagagcct caagcgcacc
     3301 cacaacacca cggacgacaa gattctgtcc ctgatcgagt gcaacaacga ggaagtcaag
     3361 caggaaaact ccaacaagaa ccccaccgtc aacagcgtcc agcgcgacta tatggccggt
     3421 gaggtcagca aggatctgac gatgcgtatg ctgcttcccc cggagatcgt caaggcgcac
     3481 gaggaaggca tcatccactt ccacgatgcc gactactatg cccagcatat gcacaactgc
     3541 gacctcgtca acctggacga tatgctgcag aacggcacgg tcatttccgg cactttgatt
     3601 gagaagccgc actccttctc caccgcctgc aacattgcca cgcagatcat cgcgcaagtt
     3661 gcgtccagcc agtacggcgg ccagagcatc agcctgaccc atctggcccc cttcgtcgat
     3721 gtcagccgca agaagatccg ccgcgatgtc gaggccgaga tgaaggatct gggcattgat
     3781 cccggcgagg aaaaaatctc cgagatcgtc gagaagcgcc tgcgcgagga aatcaagcgc
     3841 ggcgtgcaga ccatccaata ccaggtcgtc accctgatga ccaccaacgg ccaggcaccg
     3901 ttcatcacgg tgtttatgta cctgaacgag gccggtgaca accagcgtct caagtccgac
     3961 ttggccatcg tcgttgagga aatgctgcgc cagcgctacc agggtgtcaa gaacgaagcc
     4021 ggcgtctgga tcacccctgc cttccctaag ctgatttacg tcctggagga cgacaatatt
     4081 tacgagggcc agccctacta ctacctgacc aaactcgccg ccaagtgcac cgccaagcgc
     4141 atggtgcccg actatatcag cgagaagaag atgctggaat acaaggtcga tgccaacggc
     4201 gagggccact gcttcacctg catgggctgc cgcagcttcc tgacgccgta catcgacgag
     4261 aacggtaagc ccaagtatta cggccgcttc aatcagggcg ttgtcaccat caacctggtg
     4321 gatgtggctc tgtccagcgg cggcgatttt gataagttct ggcagctgtt tgacgagcgt
     4381 ttggagctgt gccacaaggc cctgatgtgc cgccataacc gcctgaaagg caccctgagc
     4441 gacgccgcgc ccatcctgtg gcagtacggc gcactggccc gcctgaagaa gggcgagccc
     4501 atcgacaagc tgctctacgg cggctattcc accatcagcc tgggctatgc gggcctgtat
     4561 gagtgctgca agtatatgac cggcaagagc cacaccgacc ccgccgccaa gccctttgcg
     4621 ctgaacgtga tgcagcatat gaatgacgcc tgcgccaagt ggaagaacca gcacaatatt
     4681 gatttcagcc tgtacggcac accgctggaa tccacgacct acaagttcgc caagtgcctg
     4741 cagaagcgtt tcggcgtggt gccgggcatc actgaccgca actatatcac aaattcctac
     4801 cacgtccacg tcaccgagca tatcgacgcc ttcaccaagc tgaaattcga gagcgagttc
     4861 cagaagctgt ccccgggcgg tgccatcagc tacgtggaag tgcccaacat gcaggataac
     4921 ctcgaggccg tcatcaaggt catgcagttc atctacgaga a
//
LOCUS       DBMDSQ010000139         4917 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_4135_length_4917_cov_1.638081, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000139 DBMDSQ010000000
VERSION     DBMDSQ010000139.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 4917)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4917)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4917
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4135_length_4917_cov_1.638081"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            80..2692
                     /gene="clpB"
                     /locus_tag="ACZPAX_06070"
     CDS             80..2692
                     /gene="clpB"
                     /locus_tag="ACZPAX_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015565255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HIR1113498.1"
                     /translation="MNIEQMTQKTREALQAAQRIAVEYSNNAVEQEHLLAALAQQQDG
                     LIPQMLQTLGVDANAFSNAALQKVEALPRVTGSGRDPEKIYISGDLDRALNAAEQQAK
                     QMKDEYISVEHVFLGILQRPCNAASEIFKTFGITTEKFMQTLSTVRGNQRVTSDNPED
                     TYNALKKYGQDLVEMARANKLDPVIGRDSEIRNVIRILSRKRKNNPVLIGEAGVGKTA
                     IAEGLAQRIVRGDVPENLKDRTVFSLDMGALVAGAKYRGEFEERLKSVLNEVKKSEGK
                     IILFIDELHTIVGAGKTDGAMDAGNLLKPMLARGELHCIGATTLDEYREYIEKDPALE
                     RRFQPVMVNEPTVEDTISILRGLKERYEVYHGVKIQDAALIAAATLSDRYITDRFLPD
                     KAIDLVDEACAMVKTELDSMPAELDEMNHRITQLQIEEASLKKETDELSKQRLVALEK
                     EMAELRDSFNSKKAQWENEKNAVNKVQSLRAEVESTKAEIEKATRTGDYAKAGELQYG
                     KLPTLQKQLEEEEKIAEEKKESSLLRDRVTDDEIARIVARWTGIPVSKLVEGEREKLL
                     RLPDTLHQRVIGQDEAVQKVSDAILRSRAGIANPNRPIGSFLFLGPTGVGKTELAKAL
                     AQALFDDEKNMVRIDMTEYMEKFSVSRLIGAPPGYVGYEEGGQLTEAVRRHPYSVVLF
                     DEVEKAHPDVFNILLQVLDDGRITDSQGRTVDFKNTVIILTSNLGSDLILEDLEKSRA
                     NGKNELSDEAKSAIDQLLKRQFRPEFLNRLDDIVYYKSLTKQEIGSIVDLMLADLRKR
                     LTDKQLNLVVTDAAKDAIIDGGYDPIYGARPLKRYIQAHVETMIAKEIIGGAHAAGDT
                     LTVDADANGQLILR"
     gene            complement(2948..3505)
                     /locus_tag="ACZPAX_06075"
     CDS             complement(2948..3505)
                     /locus_tag="ACZPAX_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0071421 - manganese ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HIR1113499.1"
                     /translation="MTLFDLILIAVSLSMDAFAVSICKGLSVRKVSLAHALTCGVWFG
                     GFQFLMPTLGYFLGDRFATLLTKFGPWVAFVLLALIGANMVKESFGGDEQLDDDYSAK
                     AMLPLAIATSIDAFAVGVSFAAMQVAIISSAALIGITTCLLSAIGVKVGALFGDKYRA
                     PAERIGGIVLLLIGVKTLLTGLGVL"
     gene            3965..4780
                     /locus_tag="ACZPAX_06080"
     CDS             3965..4780
                     /locus_tag="ACZPAX_06080"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HIR1113500.1"
                     /translation="MQYKVLALDLDGTLTNSDKIITPRTKAALQAAAQKGVCIVLASG
                     RPTVGIEPLAKELELEKYGGCILSYNGGKIIDCQTGQTLVQHAFPADLIEPVCTFSRY
                     WNVVPLTYDKNGIVTEDADAAYVKEEARINKIPVRQVENLPAEVTYPVNKLLLTGDPA
                     DMPHVEELMQQEFAGKLSICRSAPFFIETMPLGVGKDTSLEILLRAKGLTPANLMACG
                     DGWNDLPMIRLAGMGVAMGNAQPPVKAAADYQTADNDHDGVGLAVEKFILEEA"
BASE COUNT         1161 a         1434 c         1443 g          879 t
ORIGIN      
        1 cgtaagccga aaaagtttaa cactcaacaa gttagactgc caatacaatg cgctatctgc
       61 aactaaggag gtacgcacta tgaacatcga acaaatgaca caaaagaccc gcgaggcttt
      121 gcaggctgca cagcgcatcg cggtggaata ttccaacaat gcggtcgagc aggaacacct
      181 gctggcggcg ctggcccagc agcaggacgg cctgatcccc cagatgctgc aaacccttgg
      241 cgtggacgca aatgcctttt ccaatgcggc tttgcagaaa gtagaggccc tgccccgcgt
      301 gaccggctcc ggccgcgacc cggagaaaat ctatatctcc ggcgacctgg accgtgccct
      361 caacgctgcg gagcagcaag ccaagcagat gaaggatgaa tacatcagcg ttgaacacgt
      421 tttcctcgga attttgcagc gcccgtgcaa tgctgccagc gaaattttca agacttttgg
      481 catcacgact gagaaattta tgcagacgct cagcacagtg cgtggcaatc agcgggtaac
      541 gagtgataac ccggaggata cctacaacgc gctgaaaaag tacggccagg accttgtgga
      601 gatggctcgc gccaacaaac tggacccggt catcggccgt gacagcgaga tccgcaacgt
      661 catccgcatc ctgagccgta agcgcaagaa caaccccgtc ctcatcggtg aggctggtgt
      721 cggtaagact gccattgccg agggccttgc tcagcgtatc gtgcgcggcg atgtgcccga
      781 gaacctgaaa gaccgcaccg tgttcagcct ggacatgggc gcactggtcg cgggtgcaaa
      841 ataccgcggc gagtttgagg aacgcctgaa gagcgttctg aacgaggtca agaagtccga
      901 gggcaagatc atcctcttca tcgatgaact gcacaccatc gtcggcgcag gcaagaccga
      961 cggcgcgatg gacgcgggca acctgctcaa acccatgctg gcccgcggcg agctgcactg
     1021 catcggtgca accacgctgg acgagtaccg cgagtatatc gagaaagacc ctgcgctgga
     1081 acgtcgtttc cagccggtca tggtcaacga gccgaccgtc gaggatacca tttcgatcct
     1141 gcgcggtctg aaggaacgct acgaggtcta ccacggcgtc aaaattcagg acgccgcgct
     1201 gattgccgct gcgaccctgt ctgaccgcta tatcaccgac cgtttcctgc ccgataaggc
     1261 cattgacctg gtggatgaag cctgcgccat ggtcaagacg gagctggact ccatgcccgc
     1321 tgaactggat gagatgaacc atcgtatcac ccagctgcaa atcgaggaag cctctttaaa
     1381 gaaggaaacc gacgaactgt ctaagcagcg cctggtcgct ctggagaagg aaatggccga
     1441 gctgcgcgac agcttcaaca gcaaaaaagc ccagtgggag aacgagaaga acgccgtcaa
     1501 taaggtgcag agcctgcgcg ccgaggtgga gtccaccaag gctgaaatcg aaaaggccac
     1561 ccgcaccggt gattacgcca aggccggcga gctgcagtac ggcaagttgc ctaccctgca
     1621 aaaacagctg gaagaggaag aaaaaatcgc cgaggagaag aaggaatcga gcctgctgcg
     1681 tgaccgtgtg acggacgacg aaatcgcccg catcgtggct cgttggactg gtatcccggt
     1741 gtcgaaactc gtcgaaggcg agcgcgagaa gctgttgcgc ctgccggaca cgctgcatca
     1801 gcgggtcatc ggccaggacg aggctgtgca gaaggtttcc gacgctatcc tgcgttcccg
     1861 cgcgggtatc gccaacccga accgccccat cggcagcttc ctgttcctcg gccccacggg
     1921 cgtcggcaag accgaacttg ccaaggcgct ggcccaggct ctgtttgacg atgagaagaa
     1981 catggtccgt atcgacatga ccgagtacat ggagaaattc agcgtctcgc gcctgattgg
     2041 tgcgcctccg ggatacgtcg gctatgagga gggcggccaa ctgaccgagg ccgtgcgccg
     2101 ccatccgtac agcgtcgtgc tgtttgatga ggtcgaaaag gcccacccgg acgtgttcaa
     2161 catcctgttg caggtgctgg acgacggccg catcaccgac agccagggcc gcacggtgga
     2221 cttcaagaac accgtcatca tcctgacgtc gaacctcggc tctgacctga ttctggaaga
     2281 tctggaaaag agccgtgcca acggcaaaaa cgagttgtct gatgaggcaa agagcgccat
     2341 cgaccagttg ctcaagcggc agttccgccc cgagttcctg aaccgccttg acgatatcgt
     2401 ctactacaag agcctgacga aacaggaaat cggctccatc gtcgatttga tgctggccga
     2461 cctgcgcaag cgcctgaccg acaagcagct gaatctggtc gtcaccgacg cggccaagga
     2521 tgccatcatc gacggcggct atgacccgat ttacggcgcc cgccccttga agcggtacat
     2581 ccaggctcac gtcgaaacga tgatagcaaa ggagatcatc ggcggcgccc acgcggcagg
     2641 ggacaccctg accgtggatg cggatgccaa tgggcagctg attttgcgat aagacccgca
     2701 taaaataacc gctcctgggt gtgtggaatc tctccacaca cccaggagcg gttttgtttt
     2761 gtcttggcag agttgtaggg gccgcacctg tgcggcccgc aagttcccgg aagcccagcg
     2821 cttccgggtt gatgacgggc atcgacccct acgccgtagg ggcggattcc atatccgccc
     2881 gatcgtccgg gcgtttgcgg gtaagccgca ggcccggcag gccgggctcc tgcagggaac
     2941 accgatttta cagtacgccc aacccggtca gtagcgtctt gacgccaatc aaaagcagca
     3001 cgataccacc gattctctca gccggagcac ggtacttgtc gccgaacagg gcaccgacct
     3061 tcacgccgat ggcagacagc aggcaggtgg tgatgccgat gagcgcagcg gacgaaatga
     3121 tagcgacctg catagcggca aagctgacgc cgacggcaaa agcatcgata ctggtggcaa
     3181 tagccagcgg cagcatggcc ttggccgagt agtcgtcgtc cagctgctca tcgccgccga
     3241 agctctcctt gaccatgttt gcgccgatga gtgccagcag cacaaaagcg acccaggggc
     3301 cgaacttcgt cagcagcgtg gcaaagcggt cgcccagaaa atagcccagc gtgggcatca
     3361 agaactggaa cccgccgaac cagacaccgc aggtcagggc gtgggccagg ctcaccttgc
     3421 gaacggacag gcccttgcag atagaaacgg cgaatgcgtc catcgagagg ctgacagcaa
     3481 tcaggatcag gtcaaataaa gtcatggttt tactcctccg ttttggcagg ggcagacaat
     3541 aaaaaaagac ttatccgccc taaaaccggg cagataagtc tcgtcgttta aagttaagcc
     3601 agagccaagg ctcattgtgt tggcagaact gcgccgcggc tgctccaccg ctgcgctgac
     3661 tactccctta ttgcttttgg tagtgtatca tacgcaggaa tgtttgtcaa tacgttagaa
     3721 atggctaatt cctgcgatga ggcttccccc tcaggggaag ctgtccgcga agcggactga
     3781 tgaggggcaa ccttgccgtt atagcccatt tgtgtttgat tgcggtaaag ctgcccctca
     3841 tccggccctg cggggccgcc ttcccccttg ggggaaggca aggaacttta actgtcttgc
     3901 atattcccct attttagagt acaatataca caaatctgca aatttgcatc ccgggggagt
     3961 aaccatgcaa tataaagttc tggcgctgga ccttgacggt acgctgacca attccgataa
     4021 aatcatcacg ccgcgcacca aagcggcctt gcaggcggcg gcccaaaagg gcgtctgcat
     4081 tgtgctggcc agcgggcggc ccaccgtcgg cattgagccg ctggccaaag agctggaact
     4141 ggaaaaatac ggtggctgca ttttaagcta taacggcggc aagatcatcg actgccagac
     4201 cggccagaca ctggtgcagc atgccttccc ggcggacctc atcgagccgg tctgcacctt
     4261 cagccgctat tggaacgttg tgccgttgac ctacgacaaa aacggcatcg tgacggagga
     4321 cgccgacgcg gcctatgtaa aagaggaagc ccgcatcaac aaaattccgg tgcgccaggt
     4381 cgaaaacctg cccgccgaag tgacctaccc ggtcaacaag ctgctgctta ccggtgaccc
     4441 ggcggatatg ccccatgttg aggaactgat gcagcaggag ttcgcgggaa agctgtccat
     4501 ctgccgcagc gcaccgttct tcattgagac gatgcccctg ggcgtgggca aggatacctc
     4561 gctggaaatc ctgctgcggg ccaagggcct gacgcccgcc aacctgatgg cctgcggcga
     4621 cggctggaac gacctgccga tgatacgcct ggcgggtatg ggcgtcgcca tgggcaacgc
     4681 gcagccgccg gtcaaggctg ccgccgacta ccagaccgcc gacaacgatc atgacggcgt
     4741 gggcctggca gtggaaaaat ttattttaga ggaggcgtga gccgtgaatc tgattgtttt
     4801 tgatttggag tggaacatcg gctaccagcc gaaaaccttc ctgtatcacg gcacagaatt
     4861 gacgctgcgc ggtgaaatca tccagattgg cgcggtgcgc atcaacgccc ggggcga
//
LOCUS       DBMDSQ010000140         4901 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_4144_length_4901_cov_1.724375, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000140 DBMDSQ010000000
VERSION     DBMDSQ010000140.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 4901)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4901)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4901
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4144_length_4901_cov_1.724375"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..2531)
                     /locus_tag="ACZPAX_06085"
     CDS             complement(<1..2531)
                     /locus_tag="ACZPAX_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015563931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HIR1113501.1"
                     /translation="MKPFVTQVWPQFTADQQFCAAFGSVLVDRVELYRTKRQVVICLR
                     SAEPLDQALCGRLCASLAEVFAGYELQVRSYFAYPNITPEAVRLMIEELKGQGMPVNG
                     FLDKTEPVTFGDDGITIHVNAGRPILESVELPRVLAELIQERTGSLPIVRLADSGKAR
                     TEEEFEQYLQEKVPAVKFEAKETPPDFTIEGLALTNKPVKLFYGKTFKPADIRHLNDL
                     GDGGKVTVWGDVFATEVKGSRRKIYFTSITDYSGSVNLKVLGDEDADMSKWENLKPGT
                     TLIVRGNYMYDKYEHDFVILPYDVLQVERQQRQDTAPDGEKRVELHLHTKSSSMDGFN
                     DPGKIVRLAHRMGHRAIAITDHGVCQGYPEAMLATDAIHETDPNFKLIYGCEAYFVDD
                     MIPAVYGGAQMPLSGSFVVFDTETTGLDSNTERLTEIGAVFVENGKINEEKKFCTFVN
                     PGKPIPQKVVDLTGINDAMVADAPTPEEAIRAFKEFCGDNILVAHNAHSFDMLFIRKA
                     GEKTGVSWDENTYIDTLPMGQALFPGLRNYKLDTINKHLEIPPFNHHRAVDDAMALAR
                     IFEVMLTDLQEKDIHTVEAINTGLGGNKEVLKKKYYHLIILVQNQVGLKNLYRIVSAA
                     HTQYFFKKPRVPRSLLNQYREGLLLSPACEAGELYRAIVAGQPYEQLLRIADYYDYLE
                     VQPLGNNEFMVRNGQVDSIEAIKNFNRTVIKLGEDLHKPVVATGDSHFQEPEDWIYRA
                     VLQAGNGFKDADNQAPLYFRTTPDMLEDFSYLPQEKAYEIVVTNPNKIAATIDNNLRA
                     IPKGTYPPSIPGAEQELRDDTWKHAARDYGSPLPDVLQKRLKKEL"
     gene            complement(2774..3826)
                     /gene="ispG"
                     /locus_tag="ACZPAX_06090"
                     /gene_synonym="gcpE"
     CDS             complement(2774..3826)
                     /gene="ispG"
                     /locus_tag="ACZPAX_06090"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HIR1113502.1"
                     /translation="MQRQLKKEVKIGNIKIGGTNPIAVQTMLNVPVKDIAGNVAQAER
                     VAKAGCQIVRVTVPTPADAAVVAAIKEKVDIPVVADIHFDYRAALAALDAGADKIRIN
                     PGNIGGDDRVKAVADACNAKNVPIRIGVNGGSLEKHILAKYGAPVPEAMVESAMYHVR
                     LLQKYDFDNIVISIKSSNVPRMMAAYRLLAGQTDYPLHVGVTEAGGNRMGLIKSGMGI
                     GGLLMEGIGDTLRVSLTDEPENEVYAGYDILRAVGYAVAGPEIISCPTCGRTQYPMIE
                     IANEVEQRLRDEGFKKPLKIAIMGCVVNGPGEASDADIGIAGGKDEALLFIRGEKIRM
                     LRGDIVGQLLDEIHKM"
     gene            complement(3840..>4901)
                     /locus_tag="ACZPAX_06095"
     CDS             complement(3840..>4901)
                     /locus_tag="ACZPAX_06095"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF014244.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HIR1113503.1"
                     /translation="LLVFGGVVLVHELGHFMAARHCGIHVEEFSIGFGPRLWSKTKNG
                     TTYSIRLLPLGGYNLFSTPPPEDDDEAEEGEIPPKPPRKTALFPLAVSGQKFEDATAG
                     QRFFVTLCGALMNFVLGVVVMLVLVLSQGRLGSTTIADFSDGAKSAETLQTGDTILQV
                     DGKPCWTIYTLADVFDGTEKTHTMKVLRGGEVVTLPAVTVGPTLNEDGSVSYATDFRV
                     AAQKLSVHSVAVVTGEMFRYYSGAILGGFWQLFTGQAGMEELSGPIGTVSAVSQAVRY
                     GWRDVLSLMALLTINVGIFNLLPIPALDGCKLLFLAWEGLTGHPVPDSVQGFVNTAGM
                     VALVWLMVLVTLQDLTRFL"
BASE COUNT          892 a         1441 c         1530 g         1038 t
ORIGIN      
        1 tccagctcct ttttcagccg cttttgcagc acatcgggca gcgggctgcc gtagtcgcgg
       61 gccgcgtgtt tccaggtgtc gtcgcgcagt tcctgctcgg cgcccgggat gctgggcggg
      121 taggtgccct tggggatggc gcgcaggttg ttgtcgatgg tggcggcgat cttgttgggg
      181 ttcgtcacca caatttcata cgccttttcc tgcggcaggt agctgaaatc ttccagcata
      241 tcgggcgtgg tgcggaagta gagcggggcc tggttgtcgg cgtccttgaa gccgttgccg
      301 gcctgcaaaa cagcacgata gatccaatct tccggctctt gaaagtggga gtcaccggtg
      361 gcgacgaccg gcttatgcag gtcctcgccc agttttatga cggtgcggtt gaagttcttg
      421 atggcttcga tggaatccac ctggccgttg cgaaccatga actcattgtt gccaagcggc
      481 tgcacctcaa ggtaatcgta gtaatcggcg atgcgcagca gctgctcata gggctggccc
      541 gccacaatgg cgcggtacag ctcacccgct tcgcaggcgg gcgaaagcag cagaccctcg
      601 cgatactggt tcaaaagact gcggggcacg cggggctttt tgaagaaata ctgggtgtgc
      661 gccgcgctga caatgcggta gaggtttttg aggcccacct ggttttgcac gaggataatc
      721 aggtggtagt atttcttttt cagcacctct ttattgccgc cgaggccggt gttgatggcc
      781 tccaccgtgt ggatgtcctt ctcctgcaag tcggtcagca tgacttcaaa gatgcgggcc
      841 agcgccatgg catcatcgac agcgcggtga tggttgaagg gcgggatctc cagatgcttg
      901 ttgatggtgt ccagcttata gttgcgcagg cccgggaaca gcgcctggcc catgggcagc
      961 gtgtcgatat aggtattctc gtcccagctg acacccgtct tctcccccgc tttgcggatg
     1021 aacagcatat caaagctgtg ggcgttgtgg gcaacgagga tgttgtcccc gcagaactct
     1081 ttgaaggcgc ggatggcttc ctccggcgtg ggggcgtcgg cgaccatggc gtcattgatg
     1141 ccggtcaggt caacgacctt ctgcgggatg ggcttgccgg ggttgacgaa ggtgcagaac
     1201 ttcttttctt cgttgatctt accgttttcc acaaagacgg cgccgatctc agtcagccgc
     1261 tcggtgttgg aatcaaggcc ggtcgtctcg gtatcgaaca cgacaaacga gccggacagc
     1321 ggcatctgcg ccccgccgta gacggcgggg atcatatcgt caacaaagta ggcctcgcag
     1381 ccgtaaatca gcttgaagtt cgggtccgtc tcgtggatgg cgtcggtggc aagcatggcc
     1441 tcggggtagc cctggcagac gccgtggtca gtgatggcga tagcgcggtg gcccatgcgg
     1501 tgggccaggc ggacaatttt gcccgggtcg ttgaagccgt ccattgagct ggacttggta
     1561 tgcaggtgca gctcgacgcg cttttcgccg tcgggggcgg tgtcctggcg ctgctggcgt
     1621 tcaacctgca ggacgtcgta gggcaggatg acaaagtcgt gctcgtactt atcgtacata
     1681 tagttgccgc gcacgatcag ggtggtgccg ggcttgaggt tctcccactt ggacatatcg
     1741 gcgtcctcgt cgcccaggac tttcaggttg acggagccgg agtagtcggt gatggaggta
     1801 aagtagattt tgcggcggct gcccttgacc tcggtggcga agacatcgcc ccagactgtg
     1861 accttaccgc catcgcccaa atcgttcaga tggcggatgt cggcaggctt gaaggtcttg
     1921 ccgtagaaca gcttgaccgg cttgttcgtg agggccagtc cctcgatcgt aaagtcgggc
     1981 ggcgtctcct tggcctcaaa cttgacggcg gggactttct cctgcaagta ctgttcaaat
     2041 tcttcttccg tgcgcgcctt tccgctgtcg gcaaggcgca cgatgggcag cgaaccggtg
     2101 cgctcctgga tgagttcggc cagcacgcgg ggcagctcga cgctttccag gatcgggcgg
     2161 cccgcgttga catggatggt gatgccgtcg tcgccgaagg tgacaggctc ggtcttatcc
     2221 aaaaagccgt tgaccggcat cccctgcccc ttcagttcct cgatcatcag acgcacggcc
     2281 tcgggggtga tattcgggta agcaaaatag ctgcggacct gcagctcata cccggcgaac
     2341 acctcggcca gggacgcaca aagacgcccg cacagcgcct ggtccagggg ttcagcactg
     2401 cgcaggcaga taacgacctg tctttttgtg cggtaaagtt ccacgcggtc aacaagcacg
     2461 ctgccgaacg ccgcgcagaa ctgctggtcc gccgtaaact gcggccagac ctgagtgaca
     2521 aatggtttca aggaaatttt acctcacaag tggatagcat attataggag gcggcgtccc
     2581 cgacgccccg cgggcggata tggaatccgc ccctacggag ttttggcttt cccctcgggg
     2641 gaagctgtcc gcgaagcgga ctgatgaggg gcaacgctgc ctatattacc cgtaaatggg
     2701 ttgttgcggg tacgccgccc ctcatccggc cttcggccac cttcccccaa gggggaaggc
     2761 ttttggggag tgtttacatc ttatggatct cgtccagcag ctggccgaca atatcgcccc
     2821 tcagcatacg gatcttctca ccgcggatga agagcagggc ctcgtccttg ccgccggcaa
     2881 tgccgatatc agcgtcggat gcctcgccgg ggccgttgac aacacagccc atgatggcga
     2941 ttttcagcgg ctttttaaag ccctcgtccc gcaggcgctg ctcgacctcg ttggcaatct
     3001 cgatcatcgg gtactgggtg cggccgcagg tggggcagct gatgatctcg ggccctgcca
     3061 cggcataacc caccgcgcgc aggatatcat atccggcgta gacctcgttc tcgggttcgt
     3121 cggtcagact gacgcgcagc gtgtcgccga tgccctccat cagcagaccg ccgatgccca
     3181 tgccggactt gatcagaccc atgcggttgc cgcctgcctc ggtcacaccg acgtgcaggg
     3241 ggtaatccgt ctggcctgct agcaggcggt aggccgccat catgcggggc acgttgctgg
     3301 atttaatcga tataacgatg ttatcgaagt catatttctg caaaaggcgc acatggtaca
     3361 tggcgctttc caccatagcc tccggcacag gcgcaccgta tttcgccagg atgtgctttt
     3421 ccaggctgcc gccgttgacg ccgatgcgga tgggcacgtt cttggcgttg caggcgtcgg
     3481 ccacagcctt gacgcggtcg tcgccgccga tgttgccggg gttgatgcgg attttgtctg
     3541 cacccgcgtc cagggcggcc agcgcagcgc ggtagtcaaa gtggatatca gccacgaccg
     3601 ggatatccac cttctctttg atggcggcca cgacggcggc gtccgccggg gtgggcaccg
     3661 tcacgcggac gatctggcag cccgccttgg cgacgcgctc tgcctgggcc acattgcccg
     3721 caatatcctt gacgggcaca ttcagcatag tctgcacggc aatcgggttc gtgccgccga
     3781 ttttgatatt gccaatcttg acttcctttt tcagttgacg ctgcatcgtt gtttctcctt
     3841 tataagaatc gtgtaaggtc ctgcaatgtg accagaacca tcagccacac cagggccacc
     3901 atgcccgctg tgttgacaaa gccctgcacg ctgtccggca caggatggcc ggtcagcccc
     3961 tcccacgcga ggaagagcag cttgcagccg tccagcgccg ggatgggcag caggttaaaa
     4021 atgccgacat tgatggtcag cagcgccatc agggacagca catcccgcca gccatagcgc
     4081 acagcctggc tgaccgccga caccgtaccg atggggccgg acagttcctc catgcctgcc
     4141 tggccggtga acagctgcca gaagccgccc aggatggccc cgctgtaata gcggaacatc
     4201 tccccggtga cgacggcgac gctgtgtacg ctgagtttct gcgctgccac gcggaaatcc
     4261 gtggcatagc tgacgctgcc gtcctcgttc agcgtagggc cgactgtgac ggcgggcagg
     4321 gtcacgacct cgccgccgcg caggactttc atcgtgtggg tcttttcggt gccgtcaaag
     4381 acatcggcca gtgtgtagat ggtccagcag ggcttgccgt ccacctgtaa aatcgtatcg
     4441 ccggtctgta aagtctcggc gctcttggca ccgtcgctga aatcggcgat cgtcgtgctg
     4501 ccgagcctgc cctgcgagag gacgagcacg agcatcacca caacgccgag gacaaagttc
     4561 atcaatgcgc cgcagagcgt gacaaaaaag cgctgtccgg cggtggcatc ctcaaacttc
     4621 tggccgctga ctgccagcgg aaaaagcgcc gttttgcggg gcggtttcgg gggtatctcc
     4681 ccttcctcgg cctcatcgtc atcttccggc ggcggggtgc tgaacaggtt atagccgccc
     4741 agcggcagca gacggatgga ataggtcgtt ccgtttttcg ttttggacca caggcgcggg
     4801 ccaaagccga tggaaaattc ctcgacatgg atgccgcagt ggcgcgcggc cataaagtgg
     4861 ccgagttcgt gcaccagaac aacgccgccg aacacgagca g
//
LOCUS       DBMDSQ010000141         4835 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_4209_length_4835_cov_1.565484, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000141 DBMDSQ010000000
VERSION     DBMDSQ010000141.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 4835)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4835)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4835
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4209_length_4835_cov_1.565484"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1863)
                     /locus_tag="ACZPAX_06100"
     CDS             complement(<1..1863)
                     /locus_tag="ACZPAX_06100"
                     /inference="COORDINATES: protein motif:HMM:NF014427.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HIR1113504.1"
                     /translation="MGIDWSGTDASGNALPEGTSVDLTLTMAPEYYANADGSYRWDEL
                     KDGAKLSTHLTIDNTAPELISVRQDLTQKTLAVTAMDNRYVAAVILWNMTDTPTPVSY
                     ILPNQPEDAFGKTTDCTFDLSKLSGGEKLLLQVVDYAYNASAYQLISGEEPAEALTSV
                     TLSPESVSLLAGSNYTLEASTEPYYADDKLDWTSSAPAVVTVNNNGQIKAVSAGTATI
                     TAASHKNPEVNASCTVQVLAPNYTFRGLLTADNQVQTFEWNLADSETWEKVAAVDGIG
                     SRVTSAGASSANGIYAFDVGGSAYEIDFATGKILNTGSAPFSDEIGPVAYDDIALSRK
                     YTGTESLHGDLLTGVYSMNIIPMQDPMNGSASSYISVYDLFGYDADGFSGITSLGSIT
                     FKDPDSGEDVDAEAYYVIDNAGGIWQLMIYPQDVDYYAAYQYIPTDLGFQFPYDPNWA
                     TLCSLNVADDGELFLTSYSTQGKGASRKESTTLYWLHYDEQASRFRTAIIGSFGDIAP
                     VSVTEVHTNDSTADTQKTSIFEKALRPESLKTAKAQPISQMKAANKQTAGTLNSVSQP
                     QSTGSTSAQRSTVTLDLTAIAPTASSIMEISYNTKELTLSSVTGAATLTSWYESV"
     gene            complement(1964..2605)
                     /locus_tag="ACZPAX_06105"
     CDS             complement(1964..2605)
                     /locus_tag="ACZPAX_06105"
                     /inference="COORDINATES: protein motif:HMM:NF019869.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HIR1113505.1"
                     /translation="MTGSHAVNDFCVVTLSSLYLDIIKDRLYCDGADSAARRSAQSAL
                     WIILDAMTKVFAPILAFTCDEIWLQMPHRDGDDGRNVLLNQMSKPYADYALSDTEMAV
                     WETALRVRSDVNGVLETARADKRIGKSLEAHVALIAGDEDAKAALDAVKSVDLPEIFI
                     VSNVSTNETAPAEGAVVGQGVNFPGLTIAVTEAKGTKCPRCAAVCKSLGVVFE"
     gene            complement(2632..3282)
                     /gene="pgmB"
                     /locus_tag="ACZPAX_06110"
     CDS             complement(2632..3282)
                     /gene="pgmB"
                     /locus_tag="ACZPAX_06110"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HIR1113506.1"
                     /translation="MIRGLLFDLDGVLVNTNPLHARAWQELAEELGIPFTPADQEAFL
                     GISRAQCLEILLKKGNLSLSESEKERLCTRKNDRYRELIDTLTPAALLPGVSEFLRVA
                     RAEGYRTALDSVSKNAEFVLKKLEIMAFFDAVIDGRKIVNGKPNPEVFLKGAEALGLP
                     PEQCLVFEDSAAGIEAAHRGGMKAIGIGTARLLPSAELTIPGFSGVMPDALLAQLL"
     gene            complement(3279..>4835)
                     /locus_tag="ACZPAX_06115"
     CDS             complement(3279..>4835)
                     /locus_tag="ACZPAX_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012993993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1113507.1"
                     /translation="NLVIENNAFVTLLGPSGCGKTTTLRMIAGLETPTSGRITIGDTV
                     VFDSELGINIPANKRKVGFLFQNYALWPNMTVYQNISFGLSNIKEEMPKISFEAKNAA
                     RLAQILKNPQDVVKTLEECRDKNGKLDETKAIIKLIDTYTISQYTAQKLFGYHLEQGK
                     DVSAEVKALEEKVEAARKAQPFNENFELLKDGEVETAVRKLTKEEIDLSVRRVSRIVK
                     ISMFMDRYPAELSGGQQQRVAIARTLAPEPSVLFMDEPLSNLDAKLRLEMRYELQRLH
                     VETGSTFVYVTHDQMEAMTLATQICLMNNGVLQQYDAPLEVYNHPANLFAADFVGNPS
                     INFVEAKGWQRPEGSIELTLLDGHKAVFTPEQPLQLPQWFHRRDEELEAQAQALKARA
                     GESGYVEKSNKDETFRYHIARVNDEDDGIHEEPMLTNEDLVLGIRPEFLSITGGGNVE
                     CEIYGAMPTGMESTVKVRIGEYLLTGVVFGSTLFTIGSKHLLDITGSSVMLFDRSSGR
                     RITSGTLKLL"
BASE COUNT          903 a         1393 c         1461 g         1078 t
ORIGIN      
        1 cacggactca taccaggagg taagcgtcgc cgcgcctgtg acggaggaga gcgtcagttc
       61 tttcgtattg tacgagatct ccatgatgct gcttgccgtc ggcgcaatgg cggtcagatc
      121 aagcgtcaca gtactgcgct gcgcgcttgt gctgccggtg ctctgcggct ggctgacgct
      181 gttgagcgta cctgcggtct gcttatttgc cgccttcatc tggctgatcg gctgggcctt
      241 tgcggtcttg agcgattccg gacgcagtgc cttttcaaag atgcttgtct tttgcgtatc
      301 tgctgtggag tcgttcgtat ggacctcggt cacgctgacc ggcgcaatat cgccgaagga
      361 gccgataatg gccgtgcgga agcggctcgc ctgctcatcg tagtgcagcc agtaaagcgt
      421 cgtgctttcc ttgcgggaag cgcccttgcc ctgtgtgctg tacgaagtga ggaagagttc
      481 gccgtcatcc gcaacgttca gcgagcaaag cgtcgcccag ttggggtcat acgggaactg
      541 gaagcccaga tccgtcggaa tgtactgata ggctgcgtaa tagtcgacgt cctgcggata
      601 gatcataagc tgccagatgc cgcctgcgtt gtcgatgaca taataggctt cggcgtccac
      661 atcctcaccg ctgtcgggat ccttgaacgt gatagagccc agcgaggtga tgccggagaa
      721 gccgtccgcg tcatagccga acaggtcata cacgctgatg tagctgctgg cgctgccgtt
      781 catcggatcc tgcatcggga tgatgttcat ggagtatacg ccggtcagca gatcgccatg
      841 cagcgactcc gttccggtat atttgcggga aagggcaatg tcgtcgtatg cgacggggcc
      901 aatctcgtcc gagaacggtg cgctgccggt gttcaggatc ttgccggtgg caaaatcgat
      961 ctcatatgca ctgccgccca cgtcaaacgc ataaatgccg tttgcgctgg aagcgcctgc
     1021 cgaggtcacg cggctgccga ttccgtcaac cgctgcgacc ttttcccatg tctcgctgtc
     1081 tgcgagattc cactcgaagg tctgcacctg attgtctgct gtcaaaagtc cgcggaacgt
     1141 gtagttcggc gcaagcacct gtacggtgca ggaagcattg acttcgggat ttttatgaga
     1201 agccgccgtg attgttgcgg tgccggcgct gacggccttg atctggccgt tattgttgac
     1261 cgtcacgacc gcaggcgcgg aggacgtcca atccaactta tcgtcggcgt aatacggctc
     1321 tgtggaagct tcaagcgtat agttggaacc tgcaagcagg gagacgctct ccggggaaag
     1381 cgtgacggag gtcagcgcct ctgcgggctc ctcgccgctg atcagctgat atgcagaggc
     1441 gttgtaggca tagtccacaa cctgaagcag aagcttctcg ccgccgctca gcttggagag
     1501 atcgaacgtg cagtcggtcg tcttgccgaa tgcgtcttcc ggctgattcg gcaggatata
     1561 gctgaccggc gtcggggtat ccgtcatgtt ccagaggatg acagccgcga catagcggtt
     1621 atccatggcc gtaacggcca gcgtcttctg cgtcagatcc tgacggacgg agatgagttc
     1681 cggcgcggta ttgtcgatgg tcaggtgcgt gctcaacttt gcaccatcct tgagttcatc
     1741 ccaacgatag ctgccatcgg catttgcata gtattccggg gccatcgtca gcgtaagatc
     1801 cacgctggtg ccctccggaa gcgcattgcc ggaggcgtct gtgccgctcc agtcaatgcc
     1861 cacctgaatg ccgcgcttga aatgcttcgt gaaaaccgga ctttttcagc acgcaaatgc
     1921 acccgccgct gtagcagcgg cgggtgcggg ttatcagtgc agcttattca aatacaacgc
     1981 cgagagattt gcagacggcg gcacagcgcg ggcacttcgt gcccttcgcc tcggtcacag
     2041 cgatggtcag gccggggaag ttcacgccct ggccgacaac ggcgccctct gcgggggcag
     2101 tctcgttcgt gctgacgttg gagacgatga aaatttccgg caggtcgacg gacttgaccg
     2161 catccagtgc ggcctttgca tcctcgtcgc ctgcgatcag cgccacgtgc gcttcgaggc
     2221 tcttgccgat gcgcttgtcg gcgcgggcag tctccagaac gccgttgacg tccgagcgga
     2281 cgcgcagcgc agtctcccag acggccattt cggtgtcaga cagcgcgtag tccgcatacg
     2341 gcttcgacat ctggttcagc agcacgttgc ggccgtcgtc gccgtcgcgg tgcggcatct
     2401 ggagccagat ctcgtcgcag gtgaacgcaa gaatcggcgc gaagaccttc gtcatggcat
     2461 cgaggatgat ccacagcgcg ctctgtgccg agcggcgggc ggcggaatcc gcgccgtcgc
     2521 agtacaggcg gtccttgatg atatcgagat agaggctcga aagcgtcacg acgcagaaat
     2581 cgttgacggc gtgggagccg gtcaggaatt catatcaatc tgataaaagt gttatagcaa
     2641 ctgcgccaga agcgcgtccg gcattacgcc ggagaagcct gggatggtca gctctgccga
     2701 cgggagcagc cgggccgttc cgatgccgat ggctttcatt ccgccccggt gggcggcctc
     2761 gatacctgcg gctgagtcct cgaaaaccaa gcactgctca ggtgggagtc ccagagcctc
     2821 cgctcctttg aggaacacct ccgggttggg cttgccgttt acaattttcc tgccgtcgat
     2881 gacggcgtca aaaaacgcca taatttccag ctttttcagg acaaactccg catttttgct
     2941 gacactgtca agagctgtcc ggtatccctc ggcccgggca acgcgtaaga attccgacac
     3001 gcccggcagc agcgccgccg gggtcaaagt gtcgatcagc tcccggtagc ggtcattttt
     3061 ccgggtgcac agcctctctt tttcggattc cgaaagggag aggtttccct ttttcagcag
     3121 aatttccagg cactgtgccc ggctgatgcc cagaaaggcc tcctggtctg ccggagtgaa
     3181 gggaattccc aattcttcgg caagctcctg ccacgcccgg gcgtggaggg ggttcgtgtt
     3241 caccagaact ccgtccagat cgaataacag tccccggatc acagcagctt cagggtgccg
     3301 gaggtgatgc gccgaccgct tgaccggtca aagagcatca cgctgctgcc ggtgatatcc
     3361 agcaggtgtt tgcttccgat ggtaaacagg gtgctgccga agaccacgcc ggtgagcaaa
     3421 tactctccga tgcgcacctt gacggtggat tccatgccgg tgggcatggc accgtaaatc
     3481 tcgcactcca catttccacc gccggtgatg gacaggaact ccgggcggat gcccagcacc
     3541 agatcctcgt tggtgagcat cggctcctcg tggatgccgt cgtcctcgtc gttgactctg
     3601 gcgatgtggt agcggaaggt ctcgtccttg ttgctctttt ccacgtagcc gctctctccc
     3661 gcacgggcct tcagagcctg cgcctgggct tccagctcct cgtcccgccg gtggaaccac
     3721 tggggcagct gcaatggctg ctcgggggtg aaaactgcct tgtgtccgtc cagcagcgtc
     3781 agctcaatgc tgccctcagg ccgctgccag cccttggcct ccacaaagtt gatggagggg
     3841 ttgcccacga agtctgccgc aaaaagattg gcagggtgat tgtaaacttc cagcggcgcg
     3901 tcatactgct gaaggacacc gttgttcatc aggcagatct gcgttgccag cgtcatggcc
     3961 tccatttggt cgtgggtgac gtatacgaag gtggagccgg tctccacatg gagccgctgc
     4021 aattcatagc gcatttccag acgcagtttg gcatccagat tggaaagcgg ctcgtccatg
     4081 aacagcacgc tgggttcggg agccagcgtc cgggcgatgg ccacacgctg ctgctgaccg
     4141 ccggacagct ccgcgggata gcggtccatg aacatactga ttttgacgat gcgggacacc
     4201 cggcgcacgg acaggtcgat ctcttctttg gtcagcttgc gcacagcagt ttccacctcg
     4261 ccgtccttga gaagctcaaa gttctcgttg aagggctgag cctttcgagc ggcctccacc
     4321 ttttcttcca gagcctttac ctcggcggac acgtccttac cctgctccaa atgatagccg
     4381 aacagctttt gggcagtgta ctgggagatg gtgtaggtat caatcagctt gatgatggcc
     4441 ttcgtttcgt ccagcttgcc gtttttatcc cggcattcct caagagtctt taccacatcc
     4501 tgaggatttt tgaggatctg tgccagacgg gcggcgtttt tcgcctcaaa gctgatcttc
     4561 ggcatttcct ccttgatgtt tgacaggccg aaggaaatat tctggtagac ggtcatgttg
     4621 ggccacaggg catagttctg gaagaggaag cccaccttcc gcttgttggc ggggatgttg
     4681 atgcccagct cactgtcgaa taccaccgtg tcgccgatgg tgatgcgtcc gctagtgggg
     4741 gtctccagtc ccgcgatcat ccgcagggtg gtggtcttgc cgcagccgga ggggccgagc
     4801 agggtgacaa acgcgttatt ttcaatgacc agatt
//
LOCUS       DBMDSQ010000142         4797 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_4248_length_4797_cov_1.842419, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000142 DBMDSQ010000000
VERSION     DBMDSQ010000142.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 4797)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4797)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4797
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4248_length_4797_cov_1.842419"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..304
                     /locus_tag="ACZPAX_06120"
     CDS             <1..304
                     /locus_tag="ACZPAX_06120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113508.1"
                     /translation="IDDMLNMDPAPFAEGESDTILTIDGDRGSNIAYCGHVFTEPELQ
                     PDGSLTFYQVVLTCESEDFAGWAEEETIVPDTATVTTVRLVPTENGWRVDALELPN"
     gene            1055..1366
                     /gene="rpsJ"
                     /locus_tag="ACZPAX_06125"
     CDS             1055..1366
                     /gene="rpsJ"
                     /locus_tag="ACZPAX_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HIR1113509.1"
                     /translation="MAVKEKIRIRLKSYDASLIDAAAQKIVETAKRTGARVSGPIPLP
                     TDKEVVTILRAVHKYKDSREQFETRTHKRLIDILKPSNKTVEALMSLQLPAGVDIEIK
                     L"
     gene            1532..2164
                     /gene="rplC"
                     /locus_tag="ACZPAX_06130"
     CDS             1532..2164
                     /gene="rplC"
                     /locus_tag="ACZPAX_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005938036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HIR1113510.1"
                     /translation="MQKGIIGKKLGMTQLFDANGKVVPVTIIEAGPCTVVQKKTVESD
                     GYQAVQMGYGEVSAKKVNKAAKGHFDKADVAPKRTLREFRFDDIAAVNVGDILKADVF
                     AEGDKVDVVGTSKGKGYQGVIKRFGQHRLRESHGTGPVARHAGSNGSTSSPSRVFKGK
                     RLPGHMGAVRVTVQNLSIVKVDAENNLIAIKGAIPGPKGSVVTIHDSVKA"
     gene            2179..2799
                     /gene="rplD"
                     /locus_tag="ACZPAX_06135"
     CDS             2179..2799
                     /gene="rplD"
                     /locus_tag="ACZPAX_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HIR1113511.1"
                     /translation="MAQFDVVDMNGKKVSTVELSDAVFGITPNEKAVHEAVVNFLANQ
                     RQGTQNTKIRKEVRGGGKKPWRQKGTGHARQGSIRAPQWTHGGVALGPKPRSYYYTIN
                     KKVKRLALLSALSDKAANGNLIVVDKIAADEYKTKTVVAFLAAVGAGKKNLIVNDTLD
                     AKFVKSAANIPGVQTASTGINTYDVVNADKLILSVDAAKKLEEVYA"
     gene            2799..3092
                     /gene="rplW"
                     /locus_tag="ACZPAX_06140"
     CDS             2799..3092
                     /gene="rplW"
                     /locus_tag="ACZPAX_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015564637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HIR1113512.1"
                     /translation="MKFAQDIVLAPVITENSMAGIADKKYTFKVATDATKIDIANAVE
                     ELFGVKVAKVNTISVRGRYRRQGMHAGYTAKSKKAIVTLTADSKEIEFFNSMV"
     gene            3197..4027
                     /gene="rplB"
                     /locus_tag="ACZPAX_06145"
     CDS             3197..4027
                     /gene="rplB"
                     /locus_tag="ACZPAX_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HIR1113513.1"
                     /translation="MAIKKYKPTTPGRRGMTVTDYSVLSKVDPERSLLESKKKHAGRN
                     NTGKITVRHHGGGNRVKYRVIDFKRNKFDVPATVKTLEYDPNRSAFIALVEYEDGVKS
                     YILAPDGLKVGDVIMAGPNADIKPGNALPFENIPVGTMIHNIELYPGKGGQLVRSAGV
                     MAQLMAKENGYALVRLPSGEMRNVRLNCIATVGVVSNLDHENVNLGKAGRKRHMGVRP
                     GSRGSVMNPCDHPHGGGEGRAPVGHSSPRTPWGKPALGLKTRKHKARSDKFIVKRANG
                     "
     gene            4044..4319
                     /gene="rpsS"
                     /locus_tag="ACZPAX_06150"
     CDS             4044..4319
                     /gene="rpsS"
                     /locus_tag="ACZPAX_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HIR1113514.1"
                     /translation="MSRSTKKGPYVLPSLYKKVEAMNEAGKKSVVKTWSRSSTIFPEF
                     VGHTFAVHDGRKHVPVYVTEDMVGHKLGEFAPTRKFTGHVGAKTAGK"
     gene            4337..4672
                     /gene="rplV"
                     /locus_tag="ACZPAX_06155"
     CDS             4337..4672
                     /gene="rplV"
                     /locus_tag="ACZPAX_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HIR1113515.1"
                     /translation="MEARAILRYARISPRKVSIVMDLIRNKPLDEALAILQYTPKAAC
                     EPLLKLVNSAAANAENNFNMDRNNLYVAECYVCPGPTLKRIMPRAQGRAYRILKRTSH
                     MTVVLKEKE"
     gene            4685..>4797
                     /gene="rpsC"
                     /locus_tag="ACZPAX_06160"
     CDS             4685..>4797
                     /gene="rpsC"
                     /locus_tag="ACZPAX_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015537246.1"
                     /note="forms a complex with S10 and S14; binds the lower
                     part of the 30S subunit head and the mRNA in the complete
                     ribosome to position it for translation; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HIR1113516.1"
                     /translation="MGQKVNPHGMRVGVIKDWDSRWFTSKQAFGDTLVEDH"
BASE COUNT         1138 a         1417 c         1338 g          904 t
ORIGIN      
        1 gatcgatgat atgctgaaca tggatcccgc gccttttgcg gaaggggaga gtgacacaat
       61 cctgacgatc gatggtgacc gcggcagtaa tattgcttac tgcgggcatg tctttaccga
      121 gccggaatta cagcctgacg gaagccttac gttttatcaa gtcgtgctga cttgtgagag
      181 cgaggacttc gcgggctggg cggaggagga gacgattgtg cccgataccg ccactgtaac
      241 gacggtgcgc ctggtgccga cggagaatgg ctggcgggtg gacgcgctgg agctgcccaa
      301 ctgagttgta ggggccgggc attgcggggt tgtgagcgta ggaccacggt tgagtggaac
      361 gaaaccgggg cgcgaacaat gtttgcccct gcggggcaaa caaccgcacg agcgtgtttg
      421 cttgcaaact gtcggcgctc gttgtgcgcc gacaagcgcc cggaccgcgg ccttcccgca
      481 aatgaccgtt taccttacag ctgcgggccg cacatgtgcg gcccctacag agcaataact
      541 tttcacgctg caatttgcct gaaaaacagg acttctttcc cctaaaacca tgcactctgt
      601 tgaaaacgca tacaacctct acgcttttcg gcataacgta aaaatttctc aaaaaaattt
      661 tataaaaatg ccaaaaaaca cttgcaacgc cgggaaacgt gtgctataat acatcttggc
      721 tgcggaaggc ttgtgcagat aaaacaagct gcgcaagccg cgatatgcgt tgatgcggga
      781 ggttgccgcc acatggccga taggctggcg ggtttttccg cggagtatgt ccgatcttag
      841 aaccgggcga aagaattacg gaaacaaagg gatatgtacg tgcctgcatt ctgcggggcg
      901 gaccaatgtt caccttactt tgtaatgatt ctttccggga gaactgtgca aacacggttc
      961 accggatttt gtcatgcgaa aaggttgaac acccggaaac gtgtgtgatt tatcggctcg
     1021 cctaaggcgc aaaattttat aggaggcgaa tgcaatggca gtcaaagaaa aaatcagaat
     1081 ccgtctgaag agctacgatg catccctgat cgatgctgcg gcacagaaga tcgtggagac
     1141 cgctaagcgc actggcgctc gcgtttccgg cccgatcccc ctgcccaccg acaaggaggt
     1201 cgtcacgatc ctgcgcgctg tccacaagta caaggatagc cgtgagcagt tcgagacccg
     1261 tacgcacaag cgtctgatcg acattctgaa gccgtccaac aagacggtcg aggctctcat
     1321 gagcctgcag ctccccgccg gcgttgacat cgagatcaag ctgtaatcga gtttctacat
     1381 tataaaggcc gatacgcgcg gccgtgatcc gtgaacggtc ggcgtgtaat ggaaaacacg
     1441 gtaaagcacc atctcgctgc aatgcaaaca gcggagaggt catgttgatg ggaaaccacc
     1501 aaccaataac ctttacagga ggacaaacac aatgcaaaaa ggtatcatcg gcaagaagct
     1561 gggcatgacc cagctgttcg acgccaacgg caaggtcgtc ccggtcacca tcattgaggc
     1621 cggcccctgc actgtcgtgc agaagaagac cgttgagtct gacggctacc aggctgttca
     1681 gatgggctac ggcgaagtga gcgccaagaa ggtcaacaag gccgccaagg gtcacttcga
     1741 caaggctgac gtcgctccca agcgcaccct gcgcgagttc cgctttgatg acatcgctgc
     1801 cgtcaacgtc ggcgacatcc tcaaggctga tgtcttcgca gagggcgaca aggttgacgt
     1861 tgtcggcacc agcaagggca agggctatca gggcgttatc aagcgctttg gccagcaccg
     1921 cctgcgtgag agccacggca ccggccctgt tgcacgtcat gccggttcta acggttccac
     1981 ttccagcccg tcccgcgtgt tcaagggcaa gcgtctgccc ggccacatgg gtgcggtccg
     2041 cgtgaccgtt cagaacctga gcatcgttaa agtcgatgct gagaacaatc tgatcgcgat
     2101 caagggtgcg atccccggcc cgaagggctc ggtcgtgacc atccacgaca gcgtgaaagc
     2161 gtaaaggagg aaaacacaat ggcacagttt gatgtcgtcg atatgaacgg caagaaggtt
     2221 tccaccgttg agctttccga cgccgtgttc gggatcaccc cgaacgagaa ggctgtccac
     2281 gaggcagtcg tcaacttcct ggccaaccag cgccagggca cccagaacac caagatccgc
     2341 aaggaagtcc gcggcggcgg caagaagcct tggcgccaga agggcaccgg ccacgctcgt
     2401 cagggctcta tccgcgctcc gcagtggacc cacggtggtg ttgctctcgg ccccaagccc
     2461 cgcagctact actacaccat caacaagaag gtcaagcgcc tggctctgct cagcgccctg
     2521 tctgacaagg ctgccaacgg caacctgatc gtcgtcgaca agatcgccgc cgacgagtac
     2581 aagaccaaga ccgtcgttgc tttcctggcc gctgtcggcg caggcaagaa gaacctgatc
     2641 gtcaatgaca ctctggacgc taagttcgtc aagagcgccg ccaacatccc cggtgtccag
     2701 actgcctcta ccggcatcaa cacctacgat gttgtcaacg ctgacaagct gatcctgagc
     2761 gttgacgctg ccaagaaact tgaggaggta tatgcgtgat gaaattcgca caggacatcg
     2821 ttctcgcccc cgtcatcacc gagaactcca tggctggcat cgctgacaag aagtacacct
     2881 tcaaggtcgc taccgacgcc accaagatcg acattgccaa cgctgttgaa gagctgttcg
     2941 gcgtgaaggt cgccaaggtc aacaccatct ccgtccgtgg tcgctatcgc cgccagggta
     3001 tgcacgccgg ctacactgcc aagagcaaga aggccattgt caccctgact gctgactcca
     3061 aggagatcga gttcttcaac tccatggttt gatgacctga tggccgcaag gccgtcactc
     3121 aggaccccgc cgaggggccc ggttatgggg atatgacccc gagaataatt cataagataa
     3181 caacaaggag atcaacatgg ctatcaagaa gtacaagccg actacccccg gtcgccgtgg
     3241 catgactgtt actgattaca gcgtcctgtc caaggttgat ccggagcgca gcctgctcga
     3301 gtccaagaag aagcacgccg gccgcaacaa caccggcaag atcaccgtcc gccatcacgg
     3361 cggcggcaac cgcgtcaagt atcgtgtcat cgacttcaag cgcaacaagt ttgatgtccc
     3421 cgccaccgtc aagacgctgg agtacgatcc gaaccgctcc gctttcatcg ccctcgtcga
     3481 gtacgaggac ggcgtcaaga gctacatcct ggctcctgac ggcctgaagg tcggtgacgt
     3541 tatcatggct ggtcccaacg cggacatcaa gcccggcaac gccctgccct tcgagaacat
     3601 ccccgtcggt accatgatcc acaacatcga gctctacccc ggcaagggcg gccagctggt
     3661 ccgctccgct ggcgttatgg ctcagctgat ggctaaggag aatggttacg ctctggtccg
     3721 tctgccctcc ggtgagatgc gcaacgttcg tctgaactgc atcgccaccg tcggcgttgt
     3781 ttccaacctc gaccacgaga acgtcaacct gggcaaggcc ggccgcaagc gccacatggg
     3841 cgtccgcccc ggcagccgtg gttccgtcat gaacccctgc gaccatcctc acggtggtgg
     3901 cgagggccgc gcacccgtcg gtcattccag cccgcgtact ccgtggggca agcccgctct
     3961 gggcctgaag acccgcaaac acaaggcccg cagcgacaag ttcattgtca agcgcgccaa
     4021 cggctaaggg aggtttttga acaatgtcta gaagcactaa gaagggccct tacgttctgc
     4081 cttccctgta caaaaaggtc gaggctatga acgaggctgg taagaagagc gtcgtgaaga
     4141 cctggagccg ttcctccacc atcttccccg agttcgtcgg tcacaccttc gccgttcatg
     4201 acggtcgtaa gcatgtgcct gtctacgtta ccgaggacat ggttggccac aagctgggcg
     4261 agtttgcacc tacccgcaag ttcaccggcc acgtcggcgc taagaccgct ggcaaataag
     4321 agaggaggaa catcgaatgg aagcacgggc aattctccgt tacgcccgca ttagtccccg
     4381 caaggtttcg atcgtgatgg acctcatccg taacaaaccc ctggacgaag cgctggctat
     4441 cctgcagtac accccgaagg ccgcctgcga gccccttctc aagctggtga actctgctgc
     4501 cgcaaatgcg gaaaacaact tcaacatgga tcgcaacaat ctctacgtcg cagaatgcta
     4561 cgtctgcccc ggccccacgc tcaagcgcat catgcctcgc gcacagggcc gtgcataccg
     4621 catcctgaag cgcaccagcc acatgactgt tgtgctgaaa gagaaagagt aaggaggtaa
     4681 acaaatgggc cagaaagtca atccccacgg catgcgcgtg ggtgtcatca aagattggga
     4741 cagccgctgg tttacctcta agcaggcatt cggcgacacc ctggtcgagg atcacaa
//
LOCUS       DBMDSQ010000143         4793 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_4252_length_4793_cov_1.926471, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000143 DBMDSQ010000000
VERSION     DBMDSQ010000143.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 4793)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4793)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4793
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4252_length_4793_cov_1.926471"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..866)
                     /locus_tag="ACZPAX_06165"
     CDS             complement(<1..866)
                     /locus_tag="ACZPAX_06165"
                     /inference="COORDINATES: protein motif:HMM:NF013703.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1113517.1"
                     /translation="MTTAHKSRVLDMTQGDPFRLVLQFSMPLFCSNLLQQFYNLTDTA
                     LAGHLLGAAALAQIGATAALYGLIMNFAFGMNNGLALTVSRYFGAGDENGMRRAVGWM
                     VTLSVGTSLVLTATSLLGRGALLTLLQVPAETWDGAADYLTVILLGIPLTMLYNMEAA
                     LLRAVGNSITPLLFLLFSTMLNIGLDAAFMGPLGLGVRGAAIATVLAQGISALLGVWY
                     ILRSYPELRFRPAQLKETTRHAVTHMFWAGLSMGLMSAIYNLGSVALQSSINALGSAY
                     IAAQTAARRLAEL"
     gene            complement(935..2341)
                     /locus_tag="ACZPAX_06170"
     CDS             complement(935..2341)
                     /locus_tag="ACZPAX_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015524310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1113518.1"
                     /translation="MDKTEKLTLRECLLGDRAFYRHVLLVVLPIIVQNTLTNVVSLLD
                     NVMVGQVGTLPMSGVAVVGQLLFVYNLAIWGSTSGAGIFGAQFYGRGDMDGVRHTFRF
                     KLLVSTAITAAAIMLFLAAGPALIGAYISADTSPADAAATLDYAWGYLRIMLVGLVPF
                     GLTQCYAGTLRESGQTVLPMKASMLAMVVNFIFNALLIFGLGPFPRLGVAGAAIATVL
                     SRFVELAIVATGAHRSADRYPFMRGVYHSLAIPGVLVKSIFIRSMPLLANELMWGAGQ
                     AVLLQCYSVRGIQAVAALNICNTIAQIFNEVFLSLGNATAIVVGQELGADRLTGARRT
                     AWRMITLSLASCVIMGAALAVCAPFIPRIYNTEEPIRLLATELIRMAALCMPLYGFAN
                     CEYFTLRSGGKTFITFLFDSCFTWAVSVPMAYCLSRFSGLNIVTIYLFVNGMDIIKCT
                     IGFVLVKKGVWVKNIVKE"
     gene            complement(2401..3477)
                     /locus_tag="ACZPAX_06175"
     CDS             complement(2401..3477)
                     /locus_tag="ACZPAX_06175"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008718887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HIR1113519.1"
                     /translation="MKMERIDRVRAALCAQGLTQMIVCDPKSVWYLTGVAVEPYERLL
                     ALYLPTDGEPVLFLNKLFNVPNPPCKTVWHTDADAPVAQIAAVVNAAKPLGIDKEWPA
                     KFLIPLMEAHPGMQVVLSSDCIDDCRACKDAEEQRLMREASRINDEVNEAAKHYVKVG
                     MTEREVAEFIDAQFRAHGCEGPSFTTIVSFGANAADPHHEPDGTVLKEGDCVLFDMGC
                     VKDRYCSDMTRTWFCGQPTEKQAAVHDLVRRANEAAEALIKPGVRLCDLDAAARDLIT
                     EAGYGEYFNHRLGHFIGQTDHEKGDVSSANTAVAKPGMIFSIEPGVYLPGEFGVRVED
                     LVLVTEGGCEVLNHNDKHWDVMGK"
     gene            3857..4309
                     /locus_tag="ACZPAX_06180"
     CDS             3857..4309
                     /locus_tag="ACZPAX_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015616624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIR1113520.1"
                     /translation="MAYTVGEMAKRLGVPASTLRYYDKEGLLPFVGRSSGGIRVFEEK
                     DFEWLRIIECLKKTGMSLKDIREYIELAMQGDATIAQRLEMFRKQKAVLEARMAELQQ
                     TMDTLDYKCWFYETAKARGTTDGISDLPDAKLPENLRPVREHLREVAE"
     gene            4349..4474
                     /locus_tag="ACZPAX_06185"
     CDS             4349..4474
                     /locus_tag="ACZPAX_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113521.1"
                     /translation="MAALKDKGCTGNCDSPIMVFFEGIVPFITTHLKKSRRGRHI"
BASE COUNT         1053 a         1403 c         1454 g          883 t
ORIGIN      
        1 agctcagcca ggcggcgggc ggcggtctgg gccgcaatgt aggcgctgcc cagcgcgttg
       61 atgctgcttt gcagggccac gctgcccagg ttgtagatgg cgctcatcag gcccatactt
      121 aggcccgccc agaacatatg ggtcacggca tggcgggtcg tctctttcag ctgggccggg
      181 cggaaccgca gttccgggta gctgcgcagg atgtaccaca cgcccagcag ggcgctgata
      241 ccctgtgcca gcacggtggc gatggctgcg ccgcgcacgc caaggcccag cgggcccata
      301 aaggccgcgt ccagcccgat attcagcatg gtgctgaaaa gcaaaaacag cagaggcgtg
      361 atgctgttgc ccacggcgcg cagcagcgcg gcttccatat tgtagagcat cgtcagcggg
      421 atgcccagca aaattacggt caggtagtcg gcggcaccgt cccacgtttc ggcggggacc
      481 tgcagcagag tcaacagcgc gccgcggccc agcagggaag tggcggtcag caccagcgag
      541 gtgcccaccg aaagcgtgac catccagccc acggcgcggc gcataccatt ttcatccccg
      601 gcgccgaaat atctgctgac cgtcagtgcc aggccgttgt tcatgccgaa ggcaaagttc
      661 atgataagac cgtacagtgc ggcggtcgcg ccgatctgcg ccagtgccgc cgcgcccagc
      721 aggtgcccgg caagggcggt gtcagtcaaa ttgtagaact gctgcagaag gttcgagcag
      781 aacagcggca tcgaaaattg cagcaccagg cggaatgggt cgccctgggt catatccagc
      841 acgcggcttt tgtgtgcggt ggtcatgggg gtgtacatct cctttatggg gcttgcaggg
      901 gtcgatgtct ggcgtcgccc ctacggaaga accactattc tttcacaata ttctttaccc
      961 agacgccctt cttcaccagc acaaagccga tggtgcattt gatgatgtcc atgccgttca
     1021 caaacaggta aattgtgacg atgttcagcc cgctgaagcg ggacaggcag taggccatcg
     1081 gcacggacac ggcccaggta aagcagctgt caaacaaaaa tgtgatgaac gttttgccgc
     1141 cggagcgcag cgtgaaatac tcacagttgg caaagccgta cagcggcata cacagcgcgg
     1201 ccatgcggat cagctcggtc gccagcaggc ggatgggttc ctcagtgttg tagatgcggg
     1261 ggataaaggg cgcacaaacg gccagcgcag cgcccataat aacgcagctg gcaaggctca
     1321 gggtaatcat ccgccaggcg gtgcggcggg caccggtcag gcggtccgcg ccaagctcct
     1381 ggccgacgac gatggccgtg gcattgccca gcgagaggaa cacctcatta aaaatctgtg
     1441 caatcgtgtt gcagatgttc agcgcggcca cggcctggat gccccgcacg gaatagcatt
     1501 gcagcagcac ggcctgacct gcgccccaca tcagctcatt ggcaagcagc ggcatcgacc
     1561 ggataaagat gcttttgacc agcacgccgg gaatggcaag gctgtggtac accccgcgca
     1621 taaaggggta gcggtcggcg ctgcggtggg cgccggtcgc gacgatggcc agctccacaa
     1681 agcgggaaag cacggtggcg atggccgcac cggccacgcc cagccggggg aacgggccaa
     1741 ggccgaaaat cagcagggcg ttaaagataa agttcacgac catcgccagc atactggctt
     1801 tcatcggcag cacggtctgg ccgctctcgc gcagggtacc ggcgtagcac tgggtcaggc
     1861 cgaagggcac cagtcccaca agcatgatgc gcagataccc ccacgcatag tccagcgtgg
     1921 cggcggcatc ggcgggggag gtatcggctg aaatatacgc tccaatcagc gctggcccgg
     1981 cagccagaaa cagcataatg gctgccgctg tgatggcggt ggacaccagc agtttaaagc
     2041 ggaaagtgtg gcgcacgccg tccatatcgc cgcggccgta aaactgagcg ccgaagatgc
     2101 cagcgcccga ggtgctgccc caaatggcga ggttatacac aaacagcagc tggcctacca
     2161 cggcaacgcc gctcatgggc agggtgccca cctggcctac catgacgttg tccagcaggg
     2221 agacaacgtt cgtcagggtg ttctgcacga tgatgggcag caccaccagc agcacatggc
     2281 ggtaaaaggc ccggtcgccc agcaggcatt cccgcagggt cagtttttcg gttttatcca
     2341 taaaagctcc tttttacatt gcacaaaaaa ggcttcccta tgggggaggc tttaagggag
     2401 ctacttcccc attacatccc aatgtttatc gttgtggttc agcacctcgc agccgccctc
     2461 ggtcacaagg acgagatcct cgacacggac gccgaactca ccgggcaggt agacgcccgg
     2521 ctcaatcgag aaaatcatgc cgggcttggc aacggcggtg ttggcgctgc tcacgtcgcc
     2581 cttctcgtgg tcggtctggc cgatgaaatg gcccagccgg tggttgaagt attcgccata
     2641 acccgcttcg gtgatcaaat cgcgggcggc ggcatccagg tcgcacaggc gcacgccggg
     2701 cttgataagc gcctcggcag cttcgttggc gcggcgcacc aggtcgtgta cggcggcctg
     2761 cttctctgtg ggctggccgc agaaccaggt gcgggtcatg tcgctgcaat agcggtcctt
     2821 cacgcagccc atgtcaaaca gcacgcagtc gccctccttc agcaccgtgc cgtccggctc
     2881 gtggtggggg tcagcggcgt tcgcaccgaa ggaaacgatg gtggtaaagc tcgggccttc
     2941 gcaaccgtgg gcgcggaact gggcgtcaat gaactcggcc acctcacgct cagtcatgcc
     3001 gaccttgacg tagtgcttcg cagcctcgtt gacttcatcg ttgatgcggg aggcctcacg
     3061 catgaggcgc tgttcctcgg cgtccttgca ggcgcggcag tcgtcgatgc aatcgctgga
     3121 cagcacgacc tgcatgccgg ggtgggcctc catcagcggg atcagaaact tggcgggcca
     3181 ctctttgtcg atacccagcg gcttcgcggc gttgaccacg gcggcgatct gggcgaccgg
     3241 ggcgtcagcg tcagtgtgcc aaacggtctt gcagggcgga ttcgggacat tgaacagctt
     3301 gttcaaaaac agcaccggct cgccgtcggt gggcaggtag agcgccagca gacgctcata
     3361 gggttccacg gcaacaccgg tcagatacca cacgcttttg gggtcgcaga cgatcatttg
     3421 ggtcaggccc tgtgcgcaca gcgcggcacg aacccggtca atacgttcca ttttcataag
     3481 ttcgacagtc ctttcgtatg ctttcccact tataataggt agatgagcgg gatttgtcaa
     3541 tataaaaaat aagccccaaa gtgggatctg gtgacgctta cgccccggcg cgtcggcggc
     3601 catgggggca tcgtggtttg cctaaaacac ccattgcagg ggccgggcat gccccgccgc
     3661 tacaactctg cgatgggaag ggcgtgccgc tgccaaatac gttctgctgt tcagatcact
     3721 gcttgttttg cgttttcata ggtgaattgc tccttcccgc agcttgacat ggggctgcaa
     3781 ctgccctata ctgatatcat acaagttaaa ccccggttta agtcgagggg ttctgcaaaa
     3841 agatcaggag gcaactatgg cttatacggt gggcgaaatg gcaaaacggc tgggtgtgcc
     3901 tgcatcgaca ctgcgctatt atgataagga agggctgctg ccctttgtgg ggcgttcctc
     3961 cggcggcatc cgcgtgtttg aggaaaagga cttcgagtgg ctgcgcatca ttgagtgcct
     4021 gaaaaagacc ggtatgtcgc tgaaggatat ccgcgaatac atcgagcttg ccatgcaggg
     4081 cgatgcgacg atcgcccagc ggttggaaat gttccgcaag cagaaggccg tgctggaagc
     4141 ccgcatggcc gaattgcagc agacgatgga cacgctggac tataagtgct ggttctatga
     4201 gaccgccaag gcccgcggca cgacagatgg catcagcgat ctgccggacg ccaaactgcc
     4261 cgagaacctg cgcccggtgc gcgagcacct gcgggaagtg gcggaatgaa ggccgaatca
     4321 aaacgacccg gcaggggaaa gcacaccttt ggccgcgctg aaggacaagg gctgcaccgg
     4381 caactgcgac agccccatca tggtcttctt tgagggcatt gtgccgttta ttacaactca
     4441 cctgaaaaaa tcccgccgcg gacgccatat ttagtggccc ggcggcagcc aaaacgccag
     4501 agcacagcaa aaattccgta aaaatcacgt tttcggtgtt aagcacagca catttccaaa
     4561 cgcctcccca tgtgggagac gttttttgtg cggaaaaacg ctccgcccca atagcacaca
     4621 atttgccccg cttagccacg ttaaatttta caaaatcccc ctgtaaaccc gcaaaaccgc
     4681 ccaaaatcgc accctgcacc accgcaagat atgcacggtt aaatattcgc cagaaaacga
     4741 ctgcaaaatt tgtcgtaaat gcacatattt tttataaaat aacgcagaaa tgc
//
LOCUS       DBMDSQ010000144         4768 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_4283_length_4768_cov_1.542318, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000144 DBMDSQ010000000
VERSION     DBMDSQ010000144.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 4768)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4768)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4768
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4283_length_4768_cov_1.542318"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="ACZPAX_06190"
     CDS             <1..201
                     /locus_tag="ACZPAX_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015525014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR1113522.1"
                     /translation="AFYMVDKSRSRAEGGSGIGLALCARIANAHGAKLEITSTEHVGT
                     TVTIAVPEVLPSEREADNDTQS"
     gene            185..970
                     /locus_tag="ACZPAX_06195"
     CDS             185..970
                     /locus_tag="ACZPAX_06195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113523.1"
                     /translation="MTRKADTLRPFLRCAAVVLCSLALPLAVFALWDRALLAAPHTRT
                     DGERFSAAGRADATACTLYAAEHVTKVPLGSNSLWDGGWTAGTMDGSALACARRTLRT
                     LHEAGLLCDDSYAAALSVCRLDYGSWELYTAPCGLTQLVRRPEEIYTGADTEHVYIQL
                     IFSGDDAPLYFNYQNDLGQGDTLADDAVAQYCALLGLDEFCDWQYPDWGSAVRDFGAA
                     GYSETAQVYAVANASGYSVTLSAVSMTPQTFAALNTQYGEETT"
     gene            967..2355
                     /locus_tag="ACZPAX_06200"
     CDS             967..2355
                     /locus_tag="ACZPAX_06200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113524.1"
                     /translation="MKRYLAITAALALVLTACGHAAPDSTPTLTAATETTQATAAPAE
                     PPQGIGSGALRMLTAAADGVYYQAFNDWEINYTDTMGRALVYAIDEQTGDARPVCSLP
                     GCAHDSAACPAWSNGNVTLCYGDGDEVYLLLFYYNDETSYYRWERIGADHTQRTVLAT
                     IEPGQSVVGRGVAVDDVNLYYSLLDEDNRHQTLWAVDKTSGQAQKICTWDDLADGTGE
                     YAPEMYTLLEVGGQQMTFAKMVQTNDALTKAMQVCAVNLTDGSITPRQRYERDTGNVL
                     VQGDGMEKRNLISYRNDYHILTEGSRGGLANCNYQSGEVGFVDAAVDTLTPVADGFPT
                     TRDGWECYYSLSGFADGWLVWVDECGRDEDGNGTGENTTRQYFCRDGVKTELTQQRYV
                     PGKDVRNIRILDAQQGRVLAAYDTKTGTVHDVDKDGTTYTRPMNWDVYGVIALDDLLA
                     GSTDFTPLNFAE"
     gene            complement(2680..2895)
                     /locus_tag="ACZPAX_06205"
     CDS             complement(2680..2895)
                     /locus_tag="ACZPAX_06205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113525.1"
                     /translation="MNKTLEISAMQYDFHTLLKVSDICGLTGEIGFHDTDTGYLVSFP
                     DDDGKADQRMAEYKKRLVDLENNIWNR"
     gene            3071..3988
                     /locus_tag="ACZPAX_06210"
     CDS             3071..3988
                     /locus_tag="ACZPAX_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006573919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParM/StbA family protein"
                     /protein_id="HIR1113526.1"
                     /translation="MLIAIDHGNKQVKTVHGNAIVSGVQKSKTRPYGRDVLKYGGSYY
                     TLSAQRIPYQKDKTTDERFFILSLFAIAEEIEAQGAYTSGLMPIDLAIGLPPAHFGAQ
                     NKAFVRYFKRKEPIYFSYRDKLYSILICNVQCYPQAFAAAAMILGELATVPRALILDV
                     GGFTADYLLLKNGRADLSTCDSLENGVILLYNRIRSKASSDLDILLEETDVDAILLQG
                     QGSSYGEEVAALVEYQTQEFVNDMLSALRERQLDLRTGRVIFVGGGACLLRRQIETSG
                     KVAHPVFVEDVNANAKGFEHLYRCTVTGA"
     gene            3995..4315
                     /locus_tag="ACZPAX_06215"
     CDS             3995..4315
                     /locus_tag="ACZPAX_06215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113527.1"
                     /translation="MKLKKDRGRFTVRFDMENPDHVRAVECLERQRDRGKAQYLADAI
                     LCYEKAATKSFQQTDRALIEQIVQEYLEKQSMGHIPNLQKEEPSISSPPIDLIASALA
                     DFRG"
     gene            complement(4386..>4768)
                     /locus_tag="ACZPAX_06220"
     CDS             complement(4386..>4768)
                     /locus_tag="ACZPAX_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015564820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR1113528.1"
                     /translation="DVKMMLLFTAIAALGQGPWQGDMNAVIASCSEYTWLTKHKRVDG
                     TMYSCTSLGVKLGGGLGTAITGWLLAFSNYDKALAVQPDSCINMLKLMYLVIPFVLDA
                     IITFILSRMKVEEANDKIRAEMKN"
BASE COUNT         1092 a         1323 c         1418 g          935 t
ORIGIN      
        1 gcattttata tggtggacaa gtcccgcagc cgggccgaag gcggcagcgg gattgggctg
       61 gcgctctgcg cccgcattgc aaatgcccac ggggcaaagc tggaaatcac cagcaccgag
      121 catgtcggca cgaccgtgac catcgccgtg ccggaggtgc tgccgagcga aagggaggct
      181 gacaatgaca cgcaaagctg atacgctgcg ccccttcctg cggtgcgccg ctgtggtgct
      241 gtgcagtctg gcgctgccgc tggcggtgtt cgccctgtgg gatcgcgcac tgctggccgc
      301 gccccacacc cgcacggacg gcgagcgctt ttcggcagcc ggacgggccg acgccacggc
      361 ctgcaccctc tacgccgccg agcatgtgac gaaggtgccg ctcggcagca acagcctctg
      421 ggacggcggc tggacggcgg gcacgatgga cggcagtgcg ctggcctgcg cccgccggac
      481 gctgcgcacc ctgcacgagg ccgggctgct gtgcgatgac agctatgccg ccgcgctctc
      541 ggtctgccgt ctggactacg gcagctggga gctgtacacc gctccctgcg gcctgacgca
      601 gctggtgcgc cgcccggaag agatctacac aggcgccgat accgagcatg tgtatatcca
      661 gctgattttt tccggtgatg atgcaccgct gtattttaac taccagaacg atctggggca
      721 gggcgatacg ctggccgatg acgccgtggc gcagtactgc gccctgctgg ggctggacga
      781 attttgcgac tggcagtacc cggactgggg ctcagccgtg cgggatttcg gcgcggcagg
      841 ctacagcgag accgcgcagg tctacgccgt ggcgaacgcc agcgggtaca gcgtgacgct
      901 gtccgccgtc agcatgacgc cgcagacctt tgcggcgctg aacacacaat acggggagga
      961 aaccacatga aacgatacct tgccatcact gccgctctgg cgctggtctt gactgcctgc
     1021 ggccatgccg cgccggacag cacaccgaca ctgaccgcag ccacggaaac aacgcaagcc
     1081 acggcggccc ccgccgaacc gccgcagggc atcggcagcg gggcattgcg gatgctgacc
     1141 gccgccgcag acggtgtgta ttaccaggcg ttcaacgatt gggaaatcaa ctacaccgac
     1201 acgatgggcc gtgcgctggt ctatgccatc gacgagcaga ccggagacgc ccgccccgtt
     1261 tgcagcctgc ccggctgtgc ccacgacagc gccgcctgcc ccgcatggag caacggcaac
     1321 gtgacgctct gctacggcga cggggacgaa gtctacctgc ttttgttcta ttataacgac
     1381 gagaccagct attaccgctg ggagcgcatc ggcgccgacc acacccagcg caccgtgctg
     1441 gcaaccattg agccgggcca gtccgtcgtg ggccgcggcg tggctgtgga cgatgtgaac
     1501 ctctattaca gcctgctgga cgaggataac cgccatcaga cgctgtgggc tgtggataag
     1561 accagcggtc aggcacagaa aatctgcaca tgggacgatc tggctgacgg gacaggggag
     1621 tatgcccccg aaatgtacac gctgttggaa gtaggcggtc aacagatgac ctttgccaag
     1681 atggtacaga cgaacgacgc cctgacgaag gccatgcagg tttgcgccgt gaacctaact
     1741 gacggcagca tcacgccgcg ccagcgctat gagcgcgata cgggcaatgt gctggtgcag
     1801 ggcgacggca tggaaaagcg caatctaatc agctaccgca atgactatca catcctgacc
     1861 gagggcagcc gtggcggcct ggcaaactgc aactaccaga gcggggaagt cggctttgtg
     1921 gacgcggcgg tggataccct gacccctgtg gccgacggct ttcccaccac ccgcgacggg
     1981 tgggagtgct actactccct gtccggcttt gccgacggct ggctggtgtg ggtggacgag
     2041 tgcggccgcg atgaagacgg caacggcacg ggcgaaaaca cgacccgcca gtatttctgc
     2101 cgcgacggcg tgaaaaccga gctgacccag cagcgctatg tgcctggcaa ggacgtgcgg
     2161 aatatccgca tcctggacgc tcagcagggc cgcgtgctgg ccgcctacga caccaaaacc
     2221 ggcacggtac acgacgtgga caaggacggc acgacctaca cccggccgat gaactgggac
     2281 gtctacggcg tcatagcgct ggacgacctg ctggcaggca gtacagactt tacgccgctg
     2341 aactttgcag aatgacaaaa aaatatgatc aggtacaatt tgttgcttac taggacggga
     2401 actcactgca cctgtcaaaa tgccatcgct ctggaaagcc aatattggca gtttgcgata
     2461 tcctctatta tactggcatt cttcttgaat attgctacgt tcattaccga cgttgtcctt
     2521 gatgcggcag attggcgcaa aatgtcgaca agatgaagaa gagcatataa gaggttagaa
     2581 ataccgataa atcataataa cagccccttc cgctgatgcg atttcagcag aaggggctat
     2641 tactttaatt gtattgaaat ttccccggcg ggtcacaaat taccgattcc agatgttgtt
     2701 ttcaagatcg acaagccgct tcttatactc tgccatacgc tgatcggcct tgccgtcatc
     2761 atcgggaaag ctgacgagat agccggtgtc tgtatcatgg aagccgattt cgccggtaag
     2821 gccgcatata tcggaaactt tcagcaaggt atgaaaatca tactgcatag cactgatttc
     2881 aagcgttttg ttcatggcgg actcctgtgt ttttacttac tttgcccatt atagcgcacc
     2941 cgccggggaa atgcaaacaa ataaataatg cgtattattg cgtataagaa aaaataatgc
     3001 gtaaatttgt tgggaaatga cgtattcaaa acgtataagg taaatataag catcgaaggg
     3061 agttgatgtt atgcttattg ctatcgacca cggcaacaaa caggtcaaaa ccgttcacgg
     3121 gaacgccatc gtttccggtg tacagaaaag taaaaccaga ccttacggca gggatgtact
     3181 gaaatacggc ggcagttact atacgctgtc agcgcagcgt atcccgtacc agaaggacaa
     3241 aaccacagac gagcgcttct tcattctgtc gctgtttgcc atcgcggagg aaatcgaagc
     3301 acagggagct tatacctccg ggctgatgcc cattgacctt gccatcggcc tgccgcccgc
     3361 acatttcggc gcgcagaaca aagcgttcgt gcgctacttc aagcgcaagg aacccatcta
     3421 cttctcctac cgtgacaagc tgtacagcat cctgatctgc aatgtgcagt gttatccgca
     3481 ggcgtttgca gcggcagcca tgatactggg cgagctggca acggtgccgc gtgcgctgat
     3541 tctggacgtg ggcggcttca cggcggacta tctgctgctg aagaacggcc gcgccgacct
     3601 gtctacctgt gactcgctgg agaatggtgt gattctgctg tacaaccgca tccgttctaa
     3661 agccagctcc gatttggaca ttctgctgga ggaaaccgat gtggatgcca ttctcttgca
     3721 ggggcagggg agcagttatg gggaagaagt cgccgcgctg gtagaatatc agacgcagga
     3781 gtttgtcaat gatatgctga gtgcattgcg cgagcgtcag cttgacctgc gcacgggacg
     3841 agtgattttc gtaggcggcg gtgcctgcct gctgcggcgg caaatcgaaa catccggaaa
     3901 ggtggcacac cccgtctttg tggaggacgt gaatgccaat gccaagggct ttgagcatct
     3961 ttaccgctgc acagtgacag gggcgtgatg aacaatgaag ctgaaaaagg accggggacg
     4021 ctttacggtt cgctttgata tggaaaatcc agaccatgtg cgggctgtag aatgcttgga
     4081 acggcagcgt gacaggggca aggcacagta tcttgccgat gcaattctat gctatgaaaa
     4141 ggcagcaacg aagtcttttc agcagacgga ccgcgccttg attgagcaaa tcgtgcagga
     4201 atacttagaa aagcagagca tggggcatat accgaatttg cagaaagagg aaccgtctat
     4261 atcttcgccg ccaatagatt taatagccag tgctttggcc gattttagag ggtaaagcac
     4321 tatacagaaa aagggcttcc gccagcagat gcggaagccc tttttaagaa ggagaatagt
     4381 gaatatcagt ttttcatttc ggcgcgaatc ttatcgttgg cctcctcgac cttcatgcgg
     4441 gagaggataa aggtaatgat ggcgtccagc acaaagggaa ttacgaggta catgagtttc
     4501 agcatattga tgcaggaatc cggctgcaca gccagcgcct tatcgtagtt gctgaacgcc
     4561 agcagccagc cggtgatggc ggtgccgaga ccgccgccca gcttgacacc gagcgaggtg
     4621 caggagtaca tcgtgccgtc cacgcgcttg tgcttcgtca gccaagtgta ctcggagcag
     4681 gaggcgataa cggcattcat gtcgccctgc cacgggccct gacccagtgc agcaatagcc
     4741 gtgaagagca gcatcatctt gacatcgc
//
LOCUS       DBMDSQ010000145         4755 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_4305_length_4755_cov_2.134508, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000145 DBMDSQ010000000
VERSION     DBMDSQ010000145.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 4755)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4755)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4755
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4305_length_4755_cov_2.134508"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(33..887)
                     /gene="argB"
                     /locus_tag="ACZPAX_06225"
     CDS             complement(33..887)
                     /gene="argB"
                     /locus_tag="ACZPAX_06225"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007048372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HIR1113529.1"
                     /translation="MKNEQMALLFSEATPYIQKYHGKTLVIKYGGNAMVNDELKLAVM
                     NDLVTLTLLGVRVVLVHGGGPAINQMLKRVGKESKFVNGLRYTDEETMGIVQQVLAGQ
                     VNKDLVALLKGRGVGLCGMDGHMIMCKRKSDVDLGYVGEIEKVNTTLIDHLLADSFIP
                     VIATVGMDDNGIPYNINADTAAAEIAIALHAEKLVSMTDIVGLLYDKDDESTLIPEVE
                     VSEIEGYKAAGVIAGGMLPKIEGMADAIYQGVHEAVIIDGRVPHSILLEMFSDRGAGT
                     MFYRRGNR"
     gene            complement(1124..2350)
                     /gene="argJ"
                     /locus_tag="ACZPAX_06230"
     CDS             complement(1124..2350)
                     /gene="argJ"
                     /locus_tag="ACZPAX_06230"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016320575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - L-glutamate N-acetyltransferase
                     activity, acting on acetyl-L-ornithine as donor [Evidence
                     IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HIR1113530.1"
                     /translation="MSNFKSIPGGICAPLGFSAAGVHCGIRHNHSKLDLALIKADVRC
                     AGAGCYTTNKVYGAPITVDREHLKDGYAQAIVVNSGNANTCAPGGVQLAKDTCDIVAK
                     ELGISADDVLPSSTGVIGQAMSIEPFAKGIPEAAAKLAASEAGSHAAATAIMTTDTHP
                     KEIALEFEVGGKTVRIGAIAKGSGMIHPNMATMLLFMTTDAKVEPAALQKALSTVVPA
                     TFNQISVDGDTSTNDTVLLLASGLSGAEIVDGTPDYDTFVAALTEAAEHLSRELAGDG
                     EGATKLLECTVTGAPDLATARAVSKSVIHSTLFKAAMFGADANWGRVLCAIGYTPGDF
                     DISKTSVRLKSKAGEVFVCENAAYHPYSEDEAAVVLKEDEIDILVDLGSGDATAKAWG
                     CDLTYDYVKINGDYRT"
     gene            complement(2402..3343)
                     /gene="argC"
                     /locus_tag="ACZPAX_06235"
     CDS             complement(2402..3343)
                     /gene="argC"
                     /locus_tag="ACZPAX_06235"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007048370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HIR1113531.1"
                     /translation="MGKYKIFVDGSSGTTGLRIADRLAERDEFEILKISAADRKDVHA
                     RAAVINESDLSFLCLPDDAAREVVPLLREDVRILDTSTAHRTAPGWLYGLPELHGTRE
                     ALKTATRVAVPGCHATGFITPVAPLVELGILPKTAHLSVTSLTGYSGGGKKMIAEYEG
                     ERTNEALNAPRPYGLALHHKHLPEMAAITGLETAPNFVPVVADYYAGMETMIPLDLTA
                     LGVSALQAAAALAEYYAGQPMIKVHPLCEGTDGGFLAANKLAGKDTLEIFCLANPAGT
                     QMQLISLFDNLGKGSSGAAVQCMNLMLGIEETKGLAR"
     gene            complement(3507..>4755)
                     /gene="argH"
                     /locus_tag="ACZPAX_06240"
     CDS             complement(3507..>4755)
                     /gene="argH"
                     /locus_tag="ACZPAX_06240"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005925343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - L-arginine biosynthetic process
                     via ornithine [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HIR1113532.1"
                     /translation="AMLGRQGIISQQDVDDIHKGLHSILEDLHSGALEIDPNAEDVHT
                     FVEQTLTARVGDAGKRLHTGRSRNDQVALDIRLNLRAASEHIQGQIKELINVLCDQAE
                     KGAGYVMPGYTHLQRAQPVTFGHQLMAYAWMLLRDLGRLQDATKRMDAECPLGSGALA
                     GTTYPLDRFYTAEQLGFAAPCGNSIDGVSDRDFCIELCSAMATCMMHLSRLSEEIILW
                     CSWEFKFIELDDAFTTGSSIMPQKKNPDVTELIRGKTGRVYGDLNTLLVMMKGIPLAY
                     DKDMQEDKEAIFDAVDTLELCLRTVTPMLATMTPLPANMRRAAAKGFINATDCADYLT
                     KKGMPFRDAYKCTGTMVAACIREGKTLEDLTLDEFKQYSDLFEDDVYTAIDLTTCCEG
                     RTSYGGPTKASVLKQVALVKGQL"
BASE COUNT          832 a         1494 c         1450 g          979 t
ORIGIN      
        1 gagccttccc ccatcagggg aagccttttc aatcagcggt tcccccgccg atagaacatt
       61 gtacctgcgc ctctgtcact gaacatttcc agcaaaatcg agtggggcac gcggccatcg
      121 atgatgacgg cctcgtggac gccctggtag atggcgtcgg ccatgccctc gatcttgggc
      181 agcataccgc ccgcaatgac gcctgcggcc ttgtagccct cgatttcgga gacttcgacc
      241 tcagggatca gcgtggattc atcgtcctta tcgtagagca gaccaacgat atccgtcatg
      301 ctgacgagtt tttctgcgtg cagggcaatg gcaatttcag ccgcggccgt gtctgcgttg
      361 atgttgtagg ggatgccgtt gtcgtccatg ccgacggtag caatgaccgg gatgaagctg
      421 tcggccagca ggtgatcgat cagcgtcgtg ttgacctttt caatttcgcc gacatagccc
      481 aaatccacat ccgatttgcg cttgcacatg atcatgtggc cgtccatgcc gcacaggccg
      541 acgccgcggc ctttgagcag cgcgaccagg tctttgttga cctgcccggc cagcacctgc
      601 tggacaatgc ccatggtctc ttcatcggtg tagcgcagac cgttcacaaa tttggattcc
      661 ttccccacgc gcttgagcat ctggttgatg gcaggcccgc cgccgtgtac cagcacgacc
      721 cgcacaccca gcagcgtcag cgtgaccagg tcgttcataa cggccagttt cagttcatcg
      781 ttgaccatgg cattgccgcc gtacttgata accagcgttt tgccgtggta tttttggata
      841 taaggtgtag cctcgctgaa cagcagggcc atttgttcat tcttcatcgt ttcctccatc
      901 ccctacctct aaagccttcc cccaaggggg aaggtggccg agcttgcgag gccggatgag
      961 gggcacgcct gccgcagcat ccgtctaccg gtctgccgcc gaaagcccgc ccctcatcag
     1021 tcggcttcgc cgacagcttc agtctacgcg ctaagagccg ccttcggcgg ttgcgctccg
     1081 acacgcgcct gcgggtgcag tcccgcggga gaagcctttt tcatcaggtt ctgtaatccc
     1141 cgttaatttt cacataatca taggttagat cgcagcccca ggccttggcg gtggcgtcgc
     1201 cgctgccaag gtcaacgagg atatcgatct catcctcctt cagcaccacg gcggcctcat
     1261 cctcgctgta cgggtggtag gcggcgttct cgcagacgaa cacctcgccg gccttgctct
     1321 tgaggcgcac cgacgtcttg ctgatatcga agtcccctgg cgtgtagcca atggcgcaga
     1381 gcacgcggcc ccagttagca tctgcaccga acatggcggc cttgaacaga gtggaatgga
     1441 tgacgctctt ggacaccgcg cgggcggtcg ccaggtcggg tgcgccggtc acggtgcatt
     1501 ccagcagctt ggtcgcgccc tcgccatccc cggccagctc gcggctcagg tgctcggcgg
     1561 cctcggtcag cgcagcaaca aacgtatcat agtcgggcgt accgtcaacg atttccgcgc
     1621 cggacaggcc ggaggccagc agcagcacgg tatcgttggt ggaggtatcg ccgtccacgg
     1681 aaatctggtt aaaggtagcg ggcacgacgg tggacagcgc cttctgcagg gcggcaggct
     1741 cgaccttggc gtcggtggtc atgaacagca gcatcgtcgc catgttcggg tggatcatgc
     1801 cgctgccctt ggcgatggca ccgatgcgaa cggtcttgcc gccgacctca aactccaaag
     1861 cgatttcttt cgggtgggta tcagtagtca tgatggcggt ggcggcggcg tggctgcctg
     1921 cctcgctggc ggccagcttg gcggcagctt cggggatgcc cttggcaaac ggctcaatgc
     1981 tcatggcctg gccgataacg cccgtggagg aggggagcac gtcgtcggcg ctgatgccca
     2041 gctctttcgc gacgatatcg caggtgtctt tcgccagctg cacgccgccc ggggcgcagg
     2101 tgttggcgtt gccgctattg acgacgatcg cctgggcata gccgtccttc agatgctcgc
     2161 gatccaccgt gatgggagcg ccgtaaacct tgttggtagt gtagcagcct gcgcccgcgc
     2221 agcggacatc cgccttgata agcgccaggt cgagcttgga atggttatgg cggatgccgc
     2281 aatgcacgcc cgccgcagaa aatcccagcg gcgcacagat gccgccgggg atggatttaa
     2341 agttgctcat aatatgtacc tctctcttta catctaagcc ttcccccttg ggggggagcc
     2401 tttatcgcgc cagccctttc gtttcctcta ttcccagcat caggttcatg cactgcacgg
     2461 ccgcacccga gctgcctttg cccaggttgt caaacaggct gatgagctgc atctgggtcc
     2521 cggccgggtt ggccaggcag aagatttcca gcgtgtcttt gcccgccagc ttgttggccg
     2581 ccaaaaagcc gccgtccgtg ccctcgcaca gggggtgcac tttgatcatc ggctgcccgg
     2641 cataatactc ggccagcgcg gcggcggcct gcagggccga aacgcccagc gccgtcagat
     2701 ccagcgggat catcgtctcc atgcctgcat agtaatccgc cacgaccggc acaaagttgg
     2761 gggccgtttc cagcccggtg atggccgcca tttcgggcag gtgcttatgg tgcagcgcca
     2821 agccgtaggg acgcggggcg ttcagcgctt cgttcgtgcg ctcgccctcg tattcagcaa
     2881 tcatcttttt gccgccgccg gagtagccgg tcaaactcgt cacactcaga tgagcggtct
     2941 tggggaggat ccccaactcc accagcgggg ccaccggcgt gataaacccg gtggcgtggc
     3001 agcccggcac agccacacgg gtggcggttt tcagcgcctc gcgggtgcca tggagctcgg
     3061 gcaggccgta gagccagccg ggggctgtgc ggtgcgctgt gctggtatcg agaatgcgca
     3121 catcctcgcg cagcaacggt acgacctcgc gggccgcatc gtccggcagg cagaggaacg
     3181 agaggtcgct ctcattgatg accgctgccc gggcgtggac gtccttgcgg tctgccgcgg
     3241 agattttcag aatttcaaat tcgtcccgtt ccgccagccg atccgcaatg cgcaggccgg
     3301 tggtaccgga ggaaccatcc acgaagattt tgtactttcc catacttgca ccccttacct
     3361 tataaaggct tccccttggg gggaagcagc cgcccgcagg cggctgatga ggggcgaacc
     3421 tgccaatgcc gcccgttacc gggttgctgc aaaaacgccg cccctcatcc ggccttcggc
     3481 caccttcccc cagcggggga aggcttttac agttgcccct tcaccagcgc aacctgcttc
     3541 agcacgctcg ccttggtcgg gccgccgtag ctggtacggc cctcgcagca ggtggtcagg
     3601 tcgatggcgg tatacacgtc atcctcaaac aggtcgctgt actgcttgaa ctcgtccagc
     3661 gtcaggtctt ccagcgtctt gccctcgcgg atgcaggcgg ccaccatcgt accggtacac
     3721 ttataggcat cgcggaaggg catacccttc ttggtcaggt agtcggcgca gtcggtggcg
     3781 ttgataaagc ccttggcggc ggcgcggcgc atattggcgg gcagcggggt catggtggcc
     3841 agcatcggcg tgacggtgcg caggcaaagc tccagcgtat ccaccgcatc aaaaattgcc
     3901 tctttgtctt cctgcatatc cttgtcatag gccaggggga tgcccttcat catgaccagc
     3961 agcgtgttca gatcgccgta gacgcggcct gttttgccgc ggatcagctc ggtcacatcg
     4021 gggtttttct tctgcggcat gatggacgag ccggtcgtaa acgcatcgtc cagctcaatg
     4081 aacttgaact cccagctgca ccacaggatg atctcctcgc tcaggcggga gaggtgcatc
     4141 atgcaggtgg ccatggcgct gcacagctca atgcagaaat cgcggtcgga cacgccgtcg
     4201 atggagttgc cgcagggggc tgcaaaaccc aactgctccg ccgtgtagaa gcggtccagc
     4261 gggtaggtcg tacccgccag tgcgccgctg cccagggggc actcggcgtc catgcgcttg
     4321 gtggcgtcct gcaaacggcc cagatcgcgc agcagcatcc aggcgtaggc catcagctgg
     4381 tggccgaagg tcacaggctg ggcgcgctgc aaatgggtgt agccgggcat aacgtagccc
     4441 gcacccttct cggcctggtc acaaagcacg ttgataagct ctttgatctg gccctggata
     4501 tgctcgctgg cggcacgcag gttcagccgg atatccagcg ccacctggtc gttgcggctg
     4561 cggccggtgt gcagccgctt acccgcatcg ccgacgcggg cggtcagcgt ctgctcgacg
     4621 aaggtatgca catcctcggc gttggggtcg atctccagtg cgccgctgtg cagatcctcc
     4681 aaaatagagt gcagaccttt gtggatatcg tccacatcct gttgggagat gataccctgt
     4741 ctgcccagca tggcc
//
LOCUS       DBMDSQ010000146         4753 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_4309_length_4753_cov_2.239037, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000146 DBMDSQ010000000
VERSION     DBMDSQ010000146.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 4753)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4753)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4753
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4309_length_4753_cov_2.239037"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(105..1103)
                     /locus_tag="ACZPAX_06245"
     CDS             complement(105..1103)
                     /locus_tag="ACZPAX_06245"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HIR1113533.1"
                     /translation="MPRNYRPGLDVVRLAALLPVLCYHYCIEAARLGFAVPTALIGRG
                     MADWVEIGLAWFFLLSGAALCLQWQGRFDARRYFVGRAAATYPAFWLGFAALFVYGEV
                     LHGNNADIPRWRVVFSVLGLDGYLAPVTVTFYKIGEWFLGVILILYLVFPLLLWCMET
                     PRRRRALGVLMAVLAVVWPLVCPVPWEAGHTVLGRLPAFALGVWFGNSLKNNVQPSYR
                     ICIGLAVCPLLWVDAVPRLAVLLVLAAVLFWAVYAAGQRLPRRFCPALRRLAGWCYGV
                     YLVHHVLLTLVFLRVAARFDLPLTGMFPMYLVVSFALAAVLMAVSGPISKAIKKLA"
     gene            complement(1104..1583)
                     /locus_tag="ACZPAX_06250"
     CDS             complement(1104..1583)
                     /locus_tag="ACZPAX_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="COG2426 family protein"
                     /protein_id="HIR1113534.1"
                     /translation="MLTKYLSVFFISMVPLIELRGAIPVGVAMGLPLWQVYIIAIIGN
                     MIPVPIIFFFARKVLEWGAGKPVIGKFFTFCLEKGHKGGEKLKAKAGRGLPWALLLFV
                     GIPLPGTGAWTGTLAASLLDMDFKSSVRACMGGVLLAGCIMGALSVLGVSAFGAVVA"
     gene            complement(1747..2505)
                     /locus_tag="ACZPAX_06255"
     CDS             complement(1747..2505)
                     /locus_tag="ACZPAX_06255"
                     /inference="COORDINATES: protein motif:HMM:NF012383.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HIR1113535.1"
                     /translation="MDSIKKWLPRLADALFPRRCPFCGALLGADALQGTVCPACVPEE
                     MRLQHIPPRLPEGEHSFYALSQAAAAYYYADAVRSAILRCKRGGSPWRARELADRMAV
                     LVFGALPARRVGGLPQYLGVAMLPLYNCIVPVPPRQPLPGVPGLPLLLARRLGQVLGI
                     PVETPLSVRPGTRPQKELTRDERLQNARGAFVCQPGADLTGKRVLLVDDIITTGATAS
                     ACALALLQAGATDVTVAAVAVAEELPKEKRKKTK"
     gene            complement(2492..2761)
                     /locus_tag="ACZPAX_06260"
     CDS             complement(2492..2761)
                     /locus_tag="ACZPAX_06260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl cyclase domain-containing protein"
                     /protein_id="HIR1113536.1"
                     /translation="MSVFDIAVSLLLAVVIIALAVRGTRELAKMKTTTDDYIQCETLA
                     RQLQSGSDYLVAFTDRENPGESIFKDADQALYQVKQNGRHGCGFY"
     gene            complement(3022..4017)
                     /locus_tag="ACZPAX_06265"
     CDS             complement(3022..4017)
                     /locus_tag="ACZPAX_06265"
                     /inference="COORDINATES: protein motif:HMM:NF024637.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HIR1113537.1"
                     /translation="MKQCPKCGKMLNDSMAFCTQCGTPLANAAAQPAPQPPQQPVWPA
                     SQPTQSAPRFAQQPTQQHYYAPANPVLNTIKQMATSPLFLAATAAQSGMVLFNIIASM
                     AGTSGLLGTVGTYLSMILSLGGYSSREASGLLDGIGSLFTGASLGATLVGQCPAILIA
                     VGLWLIYLAEAGKISTATKNTGFTLVKISTIISLAVSILGLVVAEILAIMITATAAKY
                     DDSAIGGGIIIMLLLLVVMGLGILMNSKTLSTLGVMAKTAQTAQPSDKISTYVAVISF
                     LNGIGAVIGMLGFGSVPSVLSSLCNAVVCICFAVFLFQYRDKMRALMAGRTAQTV"
BASE COUNT         1025 a         1548 c         1322 g          858 t
ORIGIN      
        1 ttcaatccgt cggggaaggg attcatccct cccgcacacc tccccatcgc cacccaccac
       61 acaaaaaagg acgcacaacc ataaaggtca tgcgtccttt atttttatgc cagctttttt
      121 atggccttgc tgattggccc gctcaccgcc atcagcactg ccgccagcgc aaaactgacg
      181 accagataca tcgggaacat cccggtcagc ggcagatcga accgcgccgc gacccgcaag
      241 aacaccagcg tcagcagcac gtgatgcaca aggtacacgc catagcacca gcccgccagc
      301 cgccgcaggg cagggcaaaa ccgccggggc agccgctgcc cggcagcata gaccgcccag
      361 aacagcaccg ccgccagcac cagcagcacg gccaggcgcg ggaccgcatc tacccacaaa
      421 agcgggcaga ccgccagtcc gatacaaatg cgataggacg gctgcacgtt gttttttaac
      481 gagttgccga accacacgcc cagcgcaaac gcaggtaaac ggcccaacac tgtgtgcccg
      541 gcctcccacg gcacagggca gaccagcggc cagacaaccg ccagcaccgc catcaagacg
      601 cccagcgccc tgcggcgccg cggtgtttcc atgcaccaca aaagcagcgg gaacacgaga
      661 tacaaaatca ggataacgcc caaaaaccac tcgccaattt tgtagaaagt gacggtcaca
      721 ggggctaaat atccgtccag ccccagcacc gaaaatacca cgcgccagcg cgggatatcg
      781 gcgttgttgc cgtgcaggac ctcgccgtag acaaacagcg ccgcaaatcc cagccagaat
      841 gccgggtagg tggccgccgc gcgccccaca aagtagcgcc gggcgtcaaa ccgtccctgc
      901 cattgcaggc agagtgccgc acccgacagc agaaaaaacc acgccagccc gatttctacc
      961 caatcggcca taccgcgccc aatgagcgcc gtcggcacgg caaagcccag ccgcgcggcc
     1021 tcgatgcaat agtggtagca cagcaccggc agcagcgccg ccagccgcac cacatccagc
     1081 ccggggcgat agttgcgggg catttacgcc acgaccgcgc cgaaggcgga gacacccagc
     1141 acgctcagcg cacccatgat gcagcccgcc agcagcacgc cgcccatgca ggcgcggaca
     1201 ctggacttga aatccatatc cagcaggctg gcggccagcg tgccggtcca ggcaccggtg
     1261 cccggcagcg ggataccaac gaacagcagc agcgcccagg gcaggccgcg gccagccttg
     1321 gccttcagct tctcgccgcc cttgtggcct ttttccaggc agaaggtgaa gaatttgcca
     1381 atgaccggct tacccgcacc ccattccagc accttgcggg caaagaagaa aatgatgggc
     1441 accgggatca tattgccgat gatggcaatg atgtacacct gccacagcgg caggcccatg
     1501 gccacgccca ccgggatcgc gccgcgcagc tcgatcaggg ggaccatcga aataaagaac
     1561 acactcaggt attttgtcag catttgaaaa cctcgcttaa acgttcaaaa ccatagtaca
     1621 gacaaattta attatacaaa atatttatgt gcttggcaag agggcacacc cataaaattt
     1681 gcccaaaaat ccggcgggca caaaaaatcc cacccccgcg ccgcgcggag gtgggcgcat
     1741 acagtctcat ttcgttttct tccgcttttc tttcggcagc tcctccgcca cagccacggc
     1801 ggccaccgtc acatccgtgg ccccggcttg cagcagggcc agcgcacagg ccgacgccgt
     1861 cgcaccggtg gtgatgatat catccaccag cagcacgcgc ttaccggtca aatccgcgcc
     1921 cggctggcag acaaacgcgc cccgggcatt ttgcaggcgc tcgtcccggg tcagctcctt
     1981 ctgcgggcgg gtgccggggc ggaccgaaag cggcgtttcc accgggatcc ccaacacctg
     2041 ccccaagcgc cgcgccagca gcagcggcag gccaggcaca ccgggcagcg gctggcgggg
     2101 cggcaccggt acaatgcagt tgtacagcgg cagcatggcg acgcccagat attgcggcag
     2161 cccgcccacc cgcctggcgg gcagcgcacc gaacaccaac accgccatgc ggtcggccag
     2221 ctccctggca cgccaggggc tgccgccgcg cttgcagcgc aaaatcgcac tgcgcaccgc
     2281 gtcggcataa tagtaggctg cggcagcctg gctcagggcg taaaagctgt gctccccttc
     2341 cggcaagcgc ggtgggatgt gctgcagccg catttcttcg ggcacgcagg ctgggcagac
     2401 ggtgccctgc aaggcatccg cgcccagcag cgccccgcaa aaggggcacc gccgtgggaa
     2461 cagcgcatcc gccagccggg gcagccactt tttaatagaa tccacagccg tgcctgccat
     2521 tctgtttgac ctggtacagc gcctgatcgg cgtccttgaa gatgctctcg ccggggttct
     2581 cgcggtcggt aaaggccacc agatagtccg acccgctttg cagctggcgg gccagcgttt
     2641 cgcactggat gtagtcgtcg gtagtggttt tcatcttggc cagttccctg gtaccgcgga
     2701 cagccagcgc gataatcacc accgccaaca gcagcgagac cgcgatatca aagacactca
     2761 cacgttgtat tctaattttt ttcaacggca gtccagcttt ccacctgaaa tttcgacgga
     2821 gcttcaggct attttgtata ttgtgtatac cagtataccg aaaatcccgc cgcattgcaa
     2881 ccaaattgct ttgggcatat cttccaaaat tgtacagtga ttttcagcag attgctcgcc
     2941 gccgcgcagc aaaaaagcgc aggggccttt ggggaaaggt tcctgcgcaa ggtagtttat
     3001 gtaggggcgc cggctgtgct tttagacagt ctgcgcggtg cggcctgcca tcagcgcacg
     3061 catcttgtcg cgatactgga agaggaacac ggcaaagcag atgcagacca ccgcattgca
     3121 caggctgctc agcaccgagg gaacgctgcc gaagcccagc atgccgatca cggcgccgat
     3181 gccgttgagg aagctgatga ccgccacgta ggttgagatt ttgtccgagg gctgcgcggt
     3241 ctgggcggtt ttcgccatca cgccaagagt gctcagggtc ttgctgttca tcaggatgcc
     3301 caggcccatg acgaccagca gaagcagcat aatgatgatg ccgccgccaa tcgcggaatc
     3361 atcgtatttt gccgcggttg cggtgatcat gatggccaaa atttccgcca cgaccaggcc
     3421 cagaatgctc acggccagac tgatgatggt gctgatcttg acgagggtaa agccggtgtt
     3481 tttcgtcgct gtgctgatct tgccagcctc cgcaaggtag atgagccaga ggcccacggc
     3541 gatcaggata gccgggcact gccccaccaa ggtcgcgccc agggacgccc cggtgaacag
     3601 cgagccgatg ccgtccagca ggccggatgc ctcccgcgag ctgtacccgc ccaggctcag
     3661 gatcatcgaa agataggtgc ccaccgtccc cagcaggccg cttgtgcccg ccatggacgc
     3721 gatgatgttg aacagcacca tgccgctctg ggcagccgtc gccgccaaaa atagcggcga
     3781 ggttgccatc tgttttatgg tgttcagcac cgggttggca ggtgcgtagt agtgctgctg
     3841 ggtgggttgc tgggcgaacc ggggcgcgga ttgtgtaggc tgcgatgcag gccagaccgg
     3901 ctgctggggc ggctggggtg caggttgggc agccgcattt gccaagggcg tgccgcactg
     3961 tgtgcagaac gccatagaat cattgagcat cttaccgcat ttaggacatt gtttcatcgt
     4021 tatttcctcc gcttctgtgt tttttactgt ggctttattc tagcacgctg tctgacgtca
     4081 gcggggcaat catggataaa acgtcattga agcgggatga atgaccgatt tctaggctcg
     4141 taggggacgg gttcatccct cccggcagct tcgcctctac cagcacttaa acttttcgca
     4201 tcccggtagg ggccgggcat gcccggccct ccactgtatc tctatcgccc ccttcccttt
     4261 cacaccgtag gggcggattc catatccgcc cgcgcacctt cgcctttacg ccctactaac
     4321 ccccgcgttg ctttactcta ctccctctac gttcgcgccg tgcgtggaat ctcccccgcc
     4381 ctaaaaacag cccgcaggcc tgtttttgcc ccgcggtacg cggacgcggg gcccgggctt
     4441 ttcgattcct gcactcctca ctcctctact ccttaacatg tataataaaa agaaaaaagc
     4501 tcaacctctt gcgaagttga gcttcctggt gacccgtgcg tccatctccc gcggactaaa
     4561 aaacaaaccg caggattgtt ttttgccgcc tgcgggcggc cgtcctgttc gattcctcgc
     4621 acggaagcac caaaaaaact ccccagccta agctggggag cattttttgg tgacccgtgc
     4681 gggaatcgaa tttaatcttg acacattgca ccatagtttt tctacgtttc aacgtgcagt
     4741 caagttttct aca
//
LOCUS       DBMDSQ010000147         4686 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_4378_length_4686_cov_1.994547, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000147 DBMDSQ010000000
VERSION     DBMDSQ010000147.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 4686)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4686)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4686
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4378_length_4686_cov_1.994547"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            208..717
                     /locus_tag="ACZPAX_06270"
     CDS             208..717
                     /locus_tag="ACZPAX_06270"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HIR1113538.1"
                     /translation="MFDKIRKMKNKKGFTLVELIVVLVILAILAALLVPALTGYIDKA
                     NNQKVVATTRSIVVAAQTEVSEKYGVVTSNNGKLKGGSLGELTVTAKNDDGIVPSDIG
                     KLAEVVSGTTPAYKNGITSAIVSYDETGHVTKVVVQQSGRTCTYDASKGTYEVGDTAP
                     VAGGSDSDT"
     gene            1299..1973
                     /locus_tag="ACZPAX_06275"
     CDS             1299..1973
                     /locus_tag="ACZPAX_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015536889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HIR1113539.1"
                     /translation="MSERLVGILVDSFGKILLPGLTVTIPLTVISFTLALIIAIVVAL
                     VQFAHVPVLQQIARFYIWVMRGTPVLVQLFVVFYGLPGVGILIEPFPAAVLVFSINEG
                     AYCAETVRAALEAVPQGQMEAGQCVGMSYLQIIRRIILPQAMRTAFPTLASFLIAMVK
                     DTSLAANITVTEMLMTTQRIVARTYEPLALYIEVGIIYLLFCTVLTRLQAVGEKYLNS
                     KGAQRS"
     gene            1977..2723
                     /locus_tag="ACZPAX_06280"
     CDS             1977..2723
                     /locus_tag="ACZPAX_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007063195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HIR1113540.1"
                     /translation="MLEIRNLRKAFGDLQVLKGIDLDVNKGDVVAILGASGSGKTTML
                     RCLNFLEKADDGTMTFDGQRVELAHMTRTQVNALRRRTGFVFQNYNLFANKTALQNVT
                     EGLIVARKMPRRQADEIARAALVRVGMADRADYYPNQLSGGQQQRVAIARAMAVDPEI
                     IYFDEPTSALDPELTGEVLAVMRSLAEEGRTMLVVTHEMGFARHVANRVVFMDGGVIV
                     EQNTAEAFFNDPQQLRTREFLQTYRQRSQL"
     gene            2723..3571
                     /locus_tag="ACZPAX_06285"
     CDS             2723..3571
                     /locus_tag="ACZPAX_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005931794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR1113541.1"
                     /translation="MKNLAKLTALTLTAALALTACGSTADSAAPAESTADTAATEETG
                     GDLLSQIQANGTITVAMEGTWAPWTYHDENDNLVGYDVEVATKIAEKLGVEPKFVEGE
                     WDGLLAGLDAGRYDIMVNGVDITPERAEKYDFSTPYAYNRTAVITQKDNDSIKTLEDL
                     NGKTTANTISSTYAELAEQYGATVTGVDDLNQTFELLLSGRIDATLNAEVTYYDYMKE
                     HPDANAKIAVLTADANEVAIPMRKGDETATLRAAIDTAIEEMRADGTLKELSEKYFGT
                     DISTNE"
     gene            3897..>4686
                     /locus_tag="ACZPAX_06290"
     CDS             3897..>4686
                     /locus_tag="ACZPAX_06290"
                     /inference="COORDINATES: protein motif:HMM:NF014026.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HIR1113542.1"
                     /translation="MTKQPKVRKPHPVLHGIGLVLSVILLVAVLIGTYVLSTMATIID
                     SFIGAPSGHYTHAEIADTKVKAEALSADVEAEGTVLVQNNDNTLPLSADTKKVNVFGW
                     ASTAWLGGGSGSGGVSTVNTDLLAALTAYGVEYNTELTDMYKDFQDGREYTRTLSAWP
                     EQSGRLYEPDINNRTYYTQSMLDNAKNYSDTAIVVIGRLAGESNDATKQQYKRTEKGG
                     DIVIDDTRTMLELTTEEENLLNYVGANYAHVVVLTTLALTSVYFV"
BASE COUNT         1045 a         1360 c         1329 g          952 t
ORIGIN      
        1 gtaaaataaa atggcgatgc ggcggcatta tcgaacggca cacgttgcaa agcaacaaaa
       61 aactttgcga aaaaggtaca aactaggcaa caaaatgttt tttgctgttt tgctgtaact
      121 ttattgaaat ttggtaaatt gtatgatatt ataacttcac agtaaaagga cggttgaagt
      181 ccttctggag aaattggagg aattataatg tttgataaaa ttcgtaaaat gaagaataag
      241 aagggcttca ccctcgtcga gctcatcgtc gttctcgtca tcctggccat cctggccgcc
      301 ctgctggtcc ccgctctgac gggctatatt gataaggcga acaatcagaa ggttgttgct
      361 accactcgta gcattgtcgt tgccgctcag acggaagttt ctgagaagta cggtgttgtt
      421 acttctaaca atggcaaact gaagggcggt tcgcttggcg agttgaccgt taccgctaag
      481 aatgatgatg gtatcgtccc cagtgatatc ggcaagctgg ccgaggtcgt ttctggtact
      541 acgcctgcct ataagaatgg cattacttct gcaatcgtta gctacgatga gactggtcat
      601 gttactaaag ttgttgttca acagagtggt cgtacttgca cttatgatgc atccaaggga
      661 acatatgagg ttggtgatac tgctcctgtt gctggcggtt ccgattctga tacctaatct
      721 gattaaataa ttgtttccca ccgtctccca ctgacggtgg gattttttat gcccattttc
      781 ccttgccgca ccgtaggggc cgggcatgcc cggccctgcc gtgtaaggca agcatccgcc
      841 cccggcaacg gtagggggcg gcgtcctcga cgccccgggg acgttaccgt aaccaccgca
      901 ttgcacggcg gttccactgg acgtagggcg gggtgccctc accccgccgc agatggagca
      961 acgccaaaac cctgcattac acaacgaacc ggggtagagg atgtaggagc aaacatagtt
     1021 cgcccgcgcc atgtacggta aacggtcgtt tgcgggaagg tcgcgggcgg catatatgct
     1081 gcccctacac cgcagagatg ttttccatcg tgcaattatg gcgcacgggc cgggcgctgc
     1141 gttcccttat ctcgcacctt taaaagcgca cacccttaaa cttcacttca aattacaccc
     1201 ttgcaaaatt cgtgccgatg tgctacaatc tcatagcata ccgttttagt gggaaatctt
     1261 cccgccaccc ctgcatcaca aaaggagaga accgccccat gtccgaacgc cttgtaggca
     1321 tccttgtgga ttcctttggt aaaatattgc tgcccggcct gaccgtcacc atcccgctga
     1381 ctgtcatttc cttcacgctg gcgctgatca ttgccattgt ggtggcgctg gtgcagtttg
     1441 ctcatgtgcc cgtcttgcag cagatcgccc ggttttacat ctgggtcatg cgcggcacgc
     1501 cggtgctggt gcagctgttt gtcgtgttct acggcctgcc cggcgttggc atccttatcg
     1561 agccgttccc cgccgcggtg ctggtctttt ccatcaatga gggcgcgtac tgcgccgaga
     1621 ccgtgcgtgc cgcgctggaa gccgtgcccc agggtcagat ggaggccggc cagtgcgtcg
     1681 gcatgagcta tttgcagatc atccgtcgta tcatcctgcc gcaggccatg cgcacggcgt
     1741 tccccacgct ggccagcttt ttgatcgcca tggtcaagga tacctcgctg gcggccaaca
     1801 tcaccgtgac cgaaatgctc atgaccaccc agcgcattgt ggcccgcacc tatgagccgc
     1861 tggcgctgta catcgaggtc ggcatcatct acctgctgtt ctgcactgtg ctgacccgcc
     1921 tgcaggccgt gggcgagaag tacctgaaca gcaagggcgc gcaaaggagt taagctatgc
     1981 tggaaatcag aaatttgcgc aaggctttcg gtgatttaca ggtgctgaaa ggcattgatc
     2041 tggacgtgaa caagggcgat gtggtcgcca ttctgggcgc aagcggcagc ggaaaaacaa
     2101 cgatgctgcg ctgcctgaac tttttggaaa aggccgacga cggcacgatg acctttgacg
     2161 gccagcgcgt ggagttggcc catatgaccc gtacccaggt caacgccctg cgccgacgca
     2221 ccgggttcgt gttccagaac tacaacctgt tcgccaacaa aacggccttg cagaacgtca
     2281 ccgaggggct gatcgtggcg cgaaaaatgc cgcgcaggca ggccgatgag atcgcccgcg
     2341 ccgcgctggt cagggtgggc atggccgacc gtgccgacta ctaccccaac cagctttccg
     2401 gcgggcagca gcagcgcgtg gcgattgccc gcgccatggc cgtagacccg gagattattt
     2461 attttgacga acccacgtcg gctcttgacc cggagctgac cggcgaagtg ttggctgtca
     2521 tgcgcagcct ggccgaggaa ggccgcacga tgctggtggt gacccacgag atgggctttg
     2581 cccgccatgt ggcgaaccgc gtggtgttta tggatggggg tgtgattgtg gagcagaaca
     2641 cagccgaggc gttcttcaac gacccgcagc agctccgcac ccgggaattt ttgcagacat
     2701 acagacaaag gagccaatta taatgaagaa ccttgccaaa ctgaccgccc tgaccctgac
     2761 cgccgcgctg gctctgacag cctgcggcag caccgccgac agcgccgccc ccgccgagag
     2821 cacggcggac accgccgcga ccgaggagac cggcggcgac ctgttgagcc agatccaggc
     2881 caacggtacc atcaccgttg ccatggaggg cacctgggcg ccctggacct accacgacga
     2941 gaacgacaac ctcgtcggct acgacgttga ggtcgcaacc aagatcgccg agaagctggg
     3001 tgtggagccg aagttcgtcg agggcgagtg ggacggcctg ctggcaggtc tggacgcggg
     3061 ccgttatgat atcatggtca acggcgttga catcaccccc gagcgcgccg agaaatacga
     3121 tttctcgacc ccctatgcct acaaccgcac cgccgtcatc acccagaagg acaacgacag
     3181 catcaagacg ctggaagatt tgaacggcaa gaccaccgcc aacacgattt ccagcaccta
     3241 cgccgagctg gccgagcagt acggcgcaac cgtgaccggt gtggacgacc tgaaccagac
     3301 ctttgagctg ctgttgagcg gccgcattga tgccacgctg aacgccgagg tgacctacta
     3361 tgattacatg aaggagcacc ccgacgcgaa cgcgaagatc gccgtgctga ctgcggacgc
     3421 gaacgaggtt gccatcccga tgcgcaaggg cgacgagacc gccaccctgc gcgctgccat
     3481 cgacaccgcc attgaggaaa tgcgcgccga cggtacgctg aaggaattga gcgagaagta
     3541 cttcgggacg gatatctcca cgaatgagta aatgaagcat cccccgccgc gttgaaacgg
     3601 cgggggaatt tttgtgtggg ggagctggat gggaaaacgg gaaattgccg gaagactgcg
     3661 gaccgggtat gcccggcccg cgctgcgtac ccgcagtggc attttgcaca aagggatgcg
     3721 accttgtctt gtgcgctttg cactacgctt tttgtaatct ttttgttaca attacagatt
     3781 gtgcgcccaa aacgacagct tatgcaaatt ttgtgccgca aagccccttt tctgctacaa
     3841 taaggtcaga acaaaggcag gggccaaaag ccccgcctta gaaggagaaa aggaatatga
     3901 caaaacaacc caaagttcgc aagccgcatc ccgtactgca cgggatcggg ctggtactgt
     3961 ccgtgatctt gctggtcgcg gtcctgatcg gtacctacgt gctgtccacg atggccacca
     4021 tcattgacag cttcatcggc gcacccagcg gccactacac gcacgccgag atcgccgaca
     4081 ccaaggtcaa ggccgaggcc ctgtccgcag acgtcgaagc cgagggcacc gtcctggtcc
     4141 agaacaacga caacaccctg ccgctgtccg ccgataccaa gaaggtcaat gtctttggct
     4201 gggcctccac tgcctggctg ggcggcggtt ccggttccgg cggcgtgtcc accgtcaaca
     4261 ccgacctgct ggccgccctg actgcctacg gtgttgagta caacaccgag ttgaccgaca
     4321 tgtacaagga cttccaggat ggccgcgagt acacccgcac gctgagcgcc tggcccgagc
     4381 agtccggccg cctgtatgag ccggatatca acaaccggac ctattacacg cagtcgatgc
     4441 tcgacaatgc taagaactac tccgacacgg ccattgtcgt catcggccgt ctggcaggcg
     4501 agagcaacga cgccaccaag cagcagtaca agcgcaccga gaagggcgga gacattgtta
     4561 tcgacgacac ccgcacgatg ctggaactga ccaccgagga agaaaacctg ctgaactatg
     4621 tcggcgcgaa ctatgcccac gtcgttgtgc tgaccacatt agcattaact tcagtatact
     4681 ttgtct
//
LOCUS       DBMDSQ010000148         4679 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_4387_length_4679_cov_1.673001, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000148 DBMDSQ010000000
VERSION     DBMDSQ010000148.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 4679)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4679)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4679
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4387_length_4679_cov_1.673001"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1993
                     /locus_tag="ACZPAX_06295"
     CDS             <1..1993
                     /locus_tag="ACZPAX_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007045867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HIR1113543.1"
                     /translation="LADADNMSLRHDIDGAIKARGVMHRDTDYIVKDGEVIIVDEFTG
                     RLMYGRRFNDGLHQAIEAKEGVTVAAESKTLATVTFQNYFRMYKKLSGMTGTASTEAD
                     EFSEIYGLNIVSIPTNKPRARKDLPDSVYKTVNGKYNAVIEQVAECHAKGQPVLVGTV
                     SVEKSEALSKLLKKKGIEHNVLNAKQHEREAEIVAQAGKQGAVTIATNMAGRGTDIML
                     GGNVTYMAKAALKKELSKELTADLAQLKDEYEHAKARAKANGTELPIPPEAGIDAKLE
                     MLMTECDGHAETEDAEILHARKRFEELCEEFAPEVKREAEVVREAGGLFIIGTERHES
                     RRIDNQLRGRAGRQGDPGASRFFLSLEDDLMRIFGGERVQNLMDSLGLDEDVPIENKL
                     ITNTIESAQKKLEASNFAIRKQVLQYDDVMNQQREIIYKQRQMVLDGEDISGKLHEMM
                     RQSIDDACANYLNGETADDWDFAGLRRHFMNWLCLPTDFNYTTEQLGDLTKDGIADEL
                     YKRGMDILTAKEKRYGAKTMRELERICLLRNVDSKWMEHIDNMDQLKQGMGLRGYGQH
                     DPVVEYRIEGFAMFDEMIASIREDAVHMLLTIEIRQQNAEPKREQIAKPTGEGAPNQA
                     GAKGAAPVRVTKIGRNDPCPCGSGLKWKKCTCKEYHPDL"
     gene            2155..2559
                     /locus_tag="ACZPAX_06300"
     CDS             2155..2559
                     /locus_tag="ACZPAX_06300"
                     /inference="COORDINATES: protein motif:HMM:NF024060.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3783 domain-containing protein"
                     /protein_id="HIR1113544.1"
                     /translation="MKARIIREPRCALLWRIDETYPNYAAITGAAKRYDVKLRAVADG
                     DLGGIVGDLCAGKPAPAFAPLIAVPDRPAIIISGLRHDNGELGAFLDLVKMGGASIPV
                     RSMVTPTSKQWTLANLLLELNTEHETVQGDKA"
     gene            2556..3575
                     /locus_tag="ACZPAX_06305"
     CDS             2556..3575
                     /locus_tag="ACZPAX_06305"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005932173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HIR1113545.1"
                     /translation="MKRSLAALALAVLLCLPLTACQKQQNLYSATWFDLFDTVAIVQG
                     YATGQDEWNAQAAAVQEDLSRYNELFDIYNHYDDVVNLYDVNAGAAAAPVAVDDELYA
                     FLRWCKDTVYPATDGATNIAAGAVLRLWHDARESDSPAPPDAEVIAAALEHIDIENLV
                     LDDDAQTVYFADPAMTLDVGAVGKGYAVEQTAQAAQARGLTSALLNIGGNVRAIGTKP
                     GGKLWTAGVENPWGDDPAYIQAVELADGDSLVISGDYQRYFEYGGVRYAHLIDLTTGW
                     PARYVSSVAVLAHADTGGLADALSTGLFCLPESTGQTLAAQNHYAVLWMHSDETTKKS
                     NDWPQ"
     gene            complement(3645..4049)
                     /gene="mgsA"
                     /locus_tag="ACZPAX_06310"
     CDS             complement(3645..4049)
                     /gene="mgsA"
                     /locus_tag="ACZPAX_06310"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009321924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HIR1113546.1"
                     /translation="MTIALIAHDSKKELMVQFCMAYRHILEQHRLLATGTTGRLIEEN
                     TGLKVTKFLPGGHGGAEQIVARIACEEVDMLLFFRDPISAKPSEPNEMNLLRICDVHN
                     VPVATNIATAEVLIHGLEHGDLDWREIINPQR"
     gene            complement(4086..>4679)
                     /locus_tag="ACZPAX_06315"
     CDS             complement(4086..>4679)
                     /locus_tag="ACZPAX_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase family protein"
                     /protein_id="HIR1113547.1"
                     /translation="IAGVYGRTVYDIEDVLCGRCEGAKAVVPSPLYPGLSVISAPYEG
                     GAVDAAPLGRLLVAMRPYFDFVILDTAAGMGAPFTAAAAVADKALLVLTPDPVALRDG
                     KIVADRLLAGARPQSAVRLVMNRVKRESFGKNAAVADLDECIDTVGAQLLAVIPESRV
                     LQLAGANGTVPPAGDPAVAAGQAMAKRLCGQRVPLTF"
BASE COUNT          999 a         1452 c         1408 g          820 t
ORIGIN      
        1 cctggccgac gccgacaata tgtcgctgcg ccatgatatt gacggtgcca tcaaggcccg
       61 cggcgttatg caccgcgaca ccgactacat cgtcaaggac ggcgaggtca tcatcgttga
      121 tgagttcacc ggacgtctga tgtacggccg ccgcttcaat gatggtctgc accaggctat
      181 tgaggccaaa gagggcgtca ccgtcgccgc cgagagcaag accctagcga ctgtcacctt
      241 ccagaactac ttccgtatgt acaaaaagct gtccggcatg accggtacag cctccaccga
      301 ggccgatgag ttcagcgaga tctacggcct gaacatagtc agcatcccca ccaataaacc
      361 ccgtgcccgt aaggatctgc ccgacagtgt ctacaagacc gtcaacggca agtacaacgc
      421 cgtcattgag caggtcgccg agtgccacgc caagggccag ccggttctgg tcggcactgt
      481 cagcgtcgag aagagcgagg ctttgtccaa gctgctgaaa aagaagggca tcgagcataa
      541 cgtgctgaac gccaagcagc acgagcgcga agccgagatc gttgcccagg ccggcaagca
      601 gggcgctgtg accatcgcca ctaacatggc tggccgtggt accgatatca tgctgggcgg
      661 caatgtcacc tacatggcca aggccgcgct gaagaaggaa ctgtccaagg aactgaccgc
      721 cgacctggcc cagctgaagg acgagtacga gcacgccaag gcccgagcca aggccaacgg
      781 aaccgaactg cccatcccgc cggaggctgg cattgacgcg aaattggaaa tgctgatgac
      841 cgagtgtgac ggccacgccg aaaccgagga cgccgaaatt ctccacgccc gcaagcgctt
      901 tgaggaactg tgcgaggagt ttgcccccga agtcaagcgc gaggccgaag tcgtccgcga
      961 ggcgggcggc ctgttcatca tcggtaccga gcgccacgag agccgccgta tcgacaacca
     1021 gctgcgtggc cgtgcaggcc gtcagggcga ccccggtgca tcccgcttct tcctgagcct
     1081 ggaagatgac ctgatgcgta ttttcggcgg cgagcgcgtg cagaacctga tggactccct
     1141 gggccttgac gaggatgtcc ccatcgagaa caagctcatt accaacacga ttgagagtgc
     1201 ccaaaaaaag ctcgaagcat cgaactttgc aattcgtaaa caggtgctgc agtatgacga
     1261 cgtcatgaac cagcagcgcg agatcatcta caagcagcgc cagatggtgc tggacggcga
     1321 ggatatctcc ggcaagctgc acgagatgat gcgccagtcc attgacgatg cctgcgcgaa
     1381 ctacctgaac ggcgagaccg ccgacgattg ggatttcgcg ggcctgcgcc gccactttat
     1441 gaactggctg tgcctgccca ccgacttcaa ctacaccacc gagcagctcg gcgacctgac
     1501 caaagatggc atcgccgacg aactgtacaa gcgcggcatg gatatcctga ccgcgaagga
     1561 gaagcgctac ggtgccaaga cgatgcgcga gttggagcgt atctgcctgc tgcgcaatgt
     1621 tgactccaag tggatggagc acattgacaa catggatcag ctcaagcagg gcatgggcct
     1681 gcgcggctac ggccagcatg acccggtggt cgagtaccgc atcgaaggct ttgccatgtt
     1741 cgatgagatg atcgcctcca tccgcgagga tgccgtgcat atgctgctga cgatcgaaat
     1801 ccgtcagcag aacgccgagc cgaagcgtga gcagatcgcc aagcccaccg gcgagggtgc
     1861 cccgaaccag gccggtgcca agggtgctgc cccggtgcgc gtgacaaaga tcggccgcaa
     1921 cgacccctgt ccctgcggca gcggcctgaa gtggaagaag tgtacctgca aagagtacca
     1981 ccccgacctg taattcatca tcaaggcgcc gcctttaatt ttgggcggtg cctttttaaa
     2041 gtggtagagt tgtaggggcc gcatatgcga ggccctcaac ttttcccgaa aatgacctgt
     2101 tcccgttatg ttgagggtcg ggtatgcccg gcccctgcga ggtagaaacc catcatgaaa
     2161 gcccgaatca tccgtgaacc ccggtgtgct ctgctttggc gcatcgatga aacctacccc
     2221 aactacgccg ccatcaccgg cgcggccaag cggtacgacg tcaaactacg tgccgtggcc
     2281 gacggcgatc tcggcggcat cgtgggcgac ctctgcgcgg gtaagcctgc cccggcgttt
     2341 gccccgctta tcgccgtgcc ggaccgcccg gctatcatca tcagcggcct gcgccacgac
     2401 aatggcgagc tgggtgcgtt cctcgacctt gtcaaaatgg gcggtgcatc catcccggtg
     2461 cgcagcatgg tcacgcccac cagcaagcaa tggacgctgg ccaacctgtt gcttgaatta
     2521 aacacggaac acgaaactgt gcagggagac aaggcgtgaa gcgcagtctt gccgcgttgg
     2581 cgctggccgt gctgctctgc ctgccgctga ccgcctgcca aaaacagcaa aatttgtaca
     2641 gcgcgacctg gtttgatctg ttcgatacgg ttgccatcgt gcaggggtac gccaccggcc
     2701 aggacgagtg gaacgctcag gctgccgccg tgcaggaaga tttatcgcgc tataacgaac
     2761 tgtttgatat ctataaccac tacgacgacg ttgtgaacct gtacgacgtc aacgccgggg
     2821 ccgctgccgc gccagtggct gttgatgacg agctgtacgc attcttgcgc tggtgtaagg
     2881 acaccgttta ccccgccacc gacggtgcga cgaacattgc ggcgggcgcg gtgctgcggc
     2941 tgtggcacga cgcgcgggaa agcgactccc ctgccccgcc cgatgcagag gtcattgcgg
     3001 cggcgctgga acatatcgac atagaaaatc tggtgctcga tgatgatgcc cagaccgtct
     3061 atttcgccga tcccgctatg acgttggacg tcggcgcggt aggcaagggt tacgctgtgg
     3121 agcagaccgc ccaagcggcc caggcccgcg gcctgacaag cgctctgctg aacatcggcg
     3181 gcaacgtccg cgccatcggc accaagcctg gcggcaagct gtggacggcg ggcgttgaaa
     3241 acccatgggg cgacgacccg gcgtacatcc aggctgtgga gctggccgac ggcgacagcc
     3301 tggtcatcag cggcgactac cagcgctatt ttgagtacgg cggtgtgcgg tacgcgcatt
     3361 tgatcgatct gaccaccggc tggcccgccc gctatgtcag cagcgtggcc gtactggccc
     3421 acgctgacac cggcggtctg gcggacgctc tgtccaccgg cttgttctgc ctgccagaat
     3481 ccaccggcca gacccttgct gcccagaacc actacgccgt cctctggatg cactccgacg
     3541 aaaccactaa aaaaagcaac gattggccgc aataaaataa gagccaccga cctttttaag
     3601 ccggtggctc ttatgatatc atggtataat acaaaacgta taattcaccg ctgcggattg
     3661 atgatttcgc gccaatccaa atcgccgtgc tccaggccgt gaatcaggac ctcggcggtg
     3721 gcaatgttgg tggcgaccgg gacgttgtgt acgtcgcaga tgcgcagcag gttcatctcg
     3781 ttcggttcgc tgggtttggc actgatgggg tcgcggaaga agagcagcat gtccacttcc
     3841 tcgcaggcga tgcgcgccac gatctgctcg gcaccgccgt ggccgccggg cagaaacttt
     3901 gttaccttca gcccagtgtt ctcctcaatc aagcggccgg tcgtgccggt agccagcaga
     3961 cggtgctgct ccaaaatatg gcggtaggcc atacaaaact gcaccatcag ctcctttttg
     4021 gagtcgtggg ctatcaatgc gatcgtcata gaaaatgcca ccatccttcg cagcagaatt
     4081 acaagttaaa aggtaagcgg gacgcgctgc ccgcacaaac gttttgccat agcctgtccg
     4141 gccgccacgg cggggtcgcc cgcaggcggc acggtgccgt tggccccggc cagctgcagt
     4201 acgcggcttt cgggtatcac ggccagcaac tgggcaccca cggtgtcaat gcactcgtcc
     4261 aagtcagcca cagcggcgtt tttgccaaag ctctcgcgct tgacgcggtt catcaccagc
     4321 cgcacggcgc tttgggggcg tgccccagcc agcagccggt ccgccacaat tttgccgtcg
     4381 cgcagggcga ccgggtcggg ggtcagcacc agcagggcct tgtccgccac ggcggcggcc
     4441 gccgtgaacg gcgcacccat cccggcggcg gtgtccaaaa tcacaaaatc gaaatagggg
     4501 cgcatggcga ccagaagccg ccccaggggc gcagcatcca ccgcgccgcc ttcataaggg
     4561 gcgctgatga ccgacagccc ggggtaaagc gggctgggca caacggcctt ggccccctcg
     4621 catcggccgc agaggacatc ctcaatgtca tagacggtgc ggccgtagac gcctgcaat
//
LOCUS       DBMDSQ010000149         4678 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_4389_length_4678_cov_1.473236, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000149 DBMDSQ010000000
VERSION     DBMDSQ010000149.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 4678)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4678)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4678
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4389_length_4678_cov_1.473236"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            316..588
                     /locus_tag="ACZPAX_06320"
     CDS             316..588
                     /locus_tag="ACZPAX_06320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113548.1"
                     /translation="MPAKLIASYRRELSALADLQDAGTVTYTLLREADAYCIRAQRDT
                     APAVECMVCDTGEERVGMLTRFLYENAVEPAQVPAVLYDLCGSAVG"
     gene            588..1409
                     /locus_tag="ACZPAX_06325"
     CDS             588..1409
                     /locus_tag="ACZPAX_06325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR1113549.1"
                     /translation="MAVLLHERPQTAGGKTRKTPNLPKTSAVVALFAFSSRLDRDTMS
                     LFAKTHSDEVRAAAIAATGQEVLELLHGGLRPNVLVLDALLTKPGVTEVLQEIGTMQL
                     DPEPAVLVLVPVPEETAARKALRLFSQYQIMLRPYGMKNLFDEVYRMGTNEDEHQLYY
                     LRQCCDDVLDAYGAPETLSGYRYAERMLLYALYADKPQQIGSLQQYVAAEEGIEIGTV
                     TSALNRMSAAMAKRGEEPYRALCLRSGRPADGVLSNGQLFKGLLKDLRDKTEQYL"
     gene            1504..2298
                     /locus_tag="ACZPAX_06330"
     CDS             1504..2298
                     /locus_tag="ACZPAX_06330"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR1113550.1"
                     /translation="MVHQEENAALLALRGGLRGRFRRSFDCMENAFEVLQDRLDHTAT
                     PAERAELTPLLEELQAQLLCLRRLGDQASDAATAALLHGTCEPRPIDLLGQLREICEI
                     LREEAAQYALPFTIELQATGLDVLPTLGDASLLNGLLTNLVSNTLAVDRAAAITLVCA
                     SGQFCYRDNGPGLPPDAKALLTDGQWSDRLLHAGGLGLPLIAAYARAMGWGLAVGEGP
                     GTELCFTLPTAPPLDGLTLESPMERRVEQQTRRLVLRRELKIIAAQ"
     gene            2506..3213
                     /gene="pyrH"
                     /locus_tag="ACZPAX_06335"
     CDS             2506..3213
                     /gene="pyrH"
                     /locus_tag="ACZPAX_06335"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HIR1113551.1"
                     /translation="MAIQYKRILLKVSGEALSGPKGTGFDEETIASICAGIKAAHDLG
                     TQIAIVVGGGNFWRGRSSGKMDRMDADKIGMLATVMNALAVKDALHQQGVDAVVMTSS
                     FMPQVAECFTSDKAIAALESGKIVVFGGGTGNPIFSTDTASALRALEISADAMFKATM
                     VDGVYDKDPHKYADAVRYDTLTFTRVLDERLAVMDSTAATLCRDNGLPILVFDLGKPE
                     NIAKACEGEMVGTLVKE"
     gene            3277..3831
                     /gene="frr"
                     /locus_tag="ACZPAX_06340"
     CDS             3277..3831
                     /gene="frr"
                     /locus_tag="ACZPAX_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015563925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HIR1113552.1"
                     /translation="MSSTTKPFEEKMNASVNHLVHELASIRAGRANPAILDKVTVDYY
                     GSPTPIQQIAAVAVAEARILTISPWDRSMLKPISKAIQTSDIGINPIDDGQVIRLVFP
                     APTEERRKQLTKDVKKQGEEAKTAVRNIRRDAMDKFKAMKKAGEITEDDQKKLEDETQ
                     KLTDKYVKLIDDTCTDKNKEIMEV"
     gene            3931..>4678
                     /locus_tag="ACZPAX_06345"
     CDS             3931..>4678
                     /locus_tag="ACZPAX_06345"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HIR1113553.1"
                     /translation="MKTRIITAVVGLGVLAVVLGFFDTFLFDLVLSAICLIAIHEVFS
                     AMGFGKKQWYLYAAAVPLTLLVMLSTSQMVRSLLLPAAFLTVLFYNVCQIAHVKTLDF
                     GKLTGFVYFSGVITFCFYSLIHLKRMLPFDQYRYDAIYFILLILCFAWGGDTAAYFAG
                     RAFGKHKLAPIVSPHKTVEGAVGGVLGSMAAGCVLTLVYSLLSARHNVLSIQVQPRHY
                     LVLLGMGAVASVLGILGDLFASSVKRQVGIK"
BASE COUNT          968 a         1394 c         1382 g          934 t
ORIGIN      
        1 ccaactgagc tacgggccca cgtcatttga ctaaattagt ttaccccttt ttgtgggaaa
       61 tgtcaagggt gaaacacaaa ttttgttgtg cgaaggcgga catatcctgt aacatacgat
      121 tttatattat catagcacca ctttcgtatc tatcttgcac atctctatat aaaatgctat
      181 aatactacaa atcttgcact tgtcgtataa aaacgtatac gcagtgtgga ttgaatcccg
      241 cacatcggtt gaattatagt agggtaataa aaccatcgca gtttacgctg cgaaagtatg
      301 tgagggggag cctttatgcc agccaaactg attgcaagct accgacggga actgtccgcg
      361 ctggcagacc tgcaagatgc cgggacagtg acctacacgc tgctgcgcga agccgatgcc
      421 tactgcatcc gtgcccagcg ggacacagcc ccggcggtgg agtgcatggt gtgtgataca
      481 ggggaggaac gcgtcgggat gctcacacgt tttctgtatg aaaatgcggt ggaacccgcg
      541 caggtgcccg ctgtcctgta cgacctctgc ggatcggcgg tgggctgatg gcggtactgt
      601 tgcatgaacg gccgcagacg gccggcggaa aaacgcgcaa gaccccgaac ctgcccaaaa
      661 cctctgctgt ggtggcgctg ttcgccttca gctcccggct ggaccgggat acgatgagcc
      721 tgtttgccaa gacccacagc gatgaagtcc gcgcggcggc cattgcggcc accgggcagg
      781 aggtgctgga actgctgcac ggcgggctgc gccccaacgt attggtgctg gacgcgctgc
      841 tgaccaagcc cggcgtgacc gaggtcttgc aggaaatcgg cacgatgcag cttgacccgg
      901 aaccagcggt tctggtgctg gtccctgtgc cggaggaaac cgccgcccgc aaggcactgc
      961 ggctgttctc ccagtaccag atcatgctgc gcccttacgg catgaaaaat ctctttgatg
     1021 aggtctaccg catgggcacg aacgaggatg agcaccagct gtactacctg cgccagtgct
     1081 gcgacgatgt gctggatgcc tacggcgcgc ccgaaacgct gagcggctac cgctatgccg
     1141 agcggatgct gctgtacgcc ctgtacgcgg acaaaccgca gcagatcggc agcctgcagc
     1201 aatacgtggc tgcggaggaa ggcatcgaga tcggcaccgt gacttcggcg ctgaatcgta
     1261 tgtcggcagc catggcaaag cggggcgaag aaccttaccg ggcgttatgc ctgcgcagcg
     1321 gccgcccggc ggacggtgta ctctccaatg ggcagctgtt caaagggctg ctgaaagact
     1381 tgcgggataa gacggaacag tacttataag gtgcgggcgg ataggggacc cgcccctgcg
     1441 gccggtgccg tgcggctcgg caccgcaccc tgaaactgaa atgaaataaa gaaggtgtcc
     1501 accatggttc atcaggagga aaacgcggca ctgctggcgc tgcggggcgg gctgcgcggg
     1561 cggttccgcc gttcgttcga ctgcatggag aatgcctttg aggtcctgca ggaccgtctg
     1621 gatcacacgg ctaccccggc ggaacgcgcc gagctgacgc cgctgctgga agaattgcag
     1681 gcgcagctgc tctgcctgcg ccgcctgggc gaccaggcat cggacgcggc cacggcggca
     1741 ttgctgcatg gcacctgtga gccgcggccc atcgacctgc tgggtcagct gcgggaaatc
     1801 tgcgaaattt tgcgggagga agccgcgcaa tacgcgctgc ccttcacgat agaattgcag
     1861 gccacagggc tggatgtgct tcccacgctg ggggatgcgt cgctgctcaa cggtctgctg
     1921 acgaaccttg tctccaacac actggctgtt gaccgggccg ccgccatcac gctggtctgt
     1981 gcgtcgggac agttctgcta ccgcgataac ggccccggtc tgccgcccga cgcaaaagcg
     2041 ctgctgaccg acggacagtg gagcgaccgt ctgctccacg cgggcgggct gggcctgccg
     2101 ctgattgccg cctatgcccg ggccatgggc tggggtctgg ccgtggggga ggggcctggc
     2161 acggaactct gcttcaccct gcccactgcg ccgccgctgg acggcctgac gctggaatcc
     2221 ccgatggagc gccgagtcga gcagcagacc cggcggctgg tgctgcgaag agaactgaaa
     2281 attatagccg cccagtagga aatgcctgtc gtatgcgccc gggctgggta tgcccggccc
     2341 ctacaacaca acaaagtttc agaccgtcca gcttgggcgg cctctttttt tcctccaccg
     2401 ctttacttta tcccggattt attgtaaaat gtactacaaa aagaatggtt tactatgcct
     2461 gcacggcagg gtagaccgcc aaaatactta caggtggtgt tttttatggc aattcagtat
     2521 aaacgtattc ttctgaaagt cagcggcgag gcactctccg gccccaaggg aaccggcttt
     2581 gatgaggaaa ccatcgcctc catctgcgcg ggcatcaagg ccgcgcacga cttgggcacc
     2641 caaatcgcca ttgtggtagg cggcggcaac ttctggcgcg gccgttccag cggcaaaatg
     2701 gaccgcatgg acgccgacaa gatcggtatg ctggccaccg ttatgaacgc actggccgtc
     2761 aaggacgccc tgcaccagca gggtgtggac gctgtcgtta tgaccagctc cttcatgccc
     2821 caggtggccg agtgcttcac cagcgacaag gccatcgccg cgctggaaag cggcaagatc
     2881 gtcgttttcg gcggcggcac aggcaaccca atcttctcca ccgataccgc cagcgccctg
     2941 cgcgcactgg aaatcagcgc cgacgccatg ttcaaggcca cgatggtgga cggcgtctac
     3001 gacaaagacc ctcacaaata tgccgacgcc gtgcgctacg acacgctgac ctttacccgt
     3061 gtgttggatg agcgcctggc tgtcatggac tccaccgctg ccaccctctg ccgtgacaac
     3121 ggtctgccca tcctcgtgtt cgaccttggc aagcccgaga acattgccaa agcctgcgag
     3181 ggcgagatgg tcggcaccct ggtcaaagag taagacccga aattgaaaat ttcatatttt
     3241 ccccctatac tactttaaaa ataaggagaa tcacctatga gcagcacgac gaaacccttt
     3301 gaagaaaaaa tgaacgccag cgtcaaccac cttgtccatg agctggcctc catccgcgca
     3361 ggccgcgcca accccgccat tctggacaaa gtcaccgtgg actactacgg cagccccacg
     3421 cccattcagc agattgccgc cgtcgccgtg gccgaggccc gcatcctgac catctccccc
     3481 tgggaccgct cgatgctcaa gcctatctcc aaggccatcc agactagcga tatcggcatc
     3541 aaccccatcg atgacggcca ggttatccgc ctggtgttcc cggccccgac cgaggaacgc
     3601 cgcaagcagc tgaccaagga cgtcaagaag cagggcgagg aagccaagac cgccgtgcgc
     3661 aacatccgcc gtgacgccat ggataagttc aaggctatga agaaggccgg cgagatcacc
     3721 gaggacgacc agaagaagct ggaagacgag acccagaagc tgaccgacaa gtacgtcaag
     3781 ctcatcgacg atacttgcac cgacaagaac aaggagatca tggaggtctg atgccttcat
     3841 gagcgatagc ctgtgcgggg atatctgcgg atatgcccgc ataggctgtt attgcaataa
     3901 aatctattcc gcgaaaggga accaccgttt atgaagaccc gcatcatcac cgccgttgtc
     3961 ggcctgggtg tcctggctgt cgttctgggc ttttttgaca cgtttttgtt cgatctggta
     4021 ctcagtgcca tctgcttgat tgccattcat gaggtttttt ccgccatggg ctttggcaaa
     4081 aagcagtggt atctctacgc tgccgctgtg ccgctgaccc tgctggtcat gctcagcacc
     4141 tcgcagatgg tgcgcagtct gctgctgccc gccgcatttt tgacggtgct gttttacaac
     4201 gtctgccaga tcgcccatgt caaaacgctg gatttcggca agctgacggg atttgtctac
     4261 ttttcgggcg ttattacgtt ctgtttttac tcgctcatcc acttaaagcg gatgctgcct
     4321 tttgaccagt accgctatga cgccatctac tttatcctgt tgatcctgtg cttcgcctgg
     4381 ggcggggata cggccgcgta tttcgcgggc cgcgctttcg gcaagcacaa gctggccccc
     4441 atcgtcagcc ctcacaagac cgtagagggc gcggtgggcg gcgtgctggg cagcatggcc
     4501 gccggctgtg tgctgacgct ggtctactcg ctgctgtccg ccaggcacaa cgtcctcagc
     4561 atccaggtac agccgcgcca ctatctggtg ctgctgggca tgggcgcggt ggcgtcggtg
     4621 ctgggcatcc tgggcgattt gtttgcctcg tcggtcaagc gccaggtcgg catcaagg
//
LOCUS       DBMDSQ010000150         4641 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_4418_length_4641_cov_1.862996, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000150 DBMDSQ010000000
VERSION     DBMDSQ010000150.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 4641)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4641)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4641
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4418_length_4641_cov_1.862996"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..>839
                     /locus_tag="ACZPAX_06350"
     CDS             <1..>839
                     /locus_tag="ACZPAX_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018213109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1113554.1"
                     /translation="HKLNEIMAVSDRCTVLRKGKYIGTVDIKDTTKEELSKMMVGRDV
                     QFAVEKKPCEPGKPILEVENLVVPSKVHKNNAVRGVSFNVRAGEIVCVAGIDGNGQSE
                     LIYGITGLEKPTSGTIKLEGKDITSAPIRERSKAGMSHIPEDRHKHGLVLDYTLADNM
                     VLQRYWQPEFQNHGFIKKAAVQEYAERLIKQYDVRSGQGPATIARSMSGGNQQKAIIA
                     REVDKEPELLVAVQPTRGLDVGAIEYIHKQIVAERDKGRAVLLVSLELDEVMNLSDRI
                     LVI"
     assembly_gap    840..849
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            954..2069
                     /locus_tag="ACZPAX_06355"
     CDS             954..2069
                     /locus_tag="ACZPAX_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1113555.1"
                     /translation="MSKQNKQPLLQRPAVVGVLASLLSIVIGLVLGFVLLVLLNPSAS
                     LGGMKAMLTTGFSNMDKLGKVLYQAAPLMMCGLSVGFAFKTGLFNIGASGQYTMGAFF
                     SLLCAIQFQMPWYVCLLAGAVGGAIWGVFPGLFKAYFNVNEVITAIMFNWIGMYLVNL
                     LLANMPNMLASGWGASNSDRTAALSAANPGAILPKGPFAALFGNSSWINISIIITILF
                     AILVWVILQKTTFGYELKACGLSRDAAQYAGINSKRNIVLSMVISGALSGIGGAIYYL
                     AGTGQYVLEKSILGMGFNGIPVALLASSNPIGTIFAALFISYIQVGGAAMQPAYSSET
                     IDIVIAVIIYLAAFALLMRGVIAKAVSGRKEKGGAAK"
     gene            2066..3058
                     /locus_tag="ACZPAX_06360"
     CDS             2066..3058
                     /locus_tag="ACZPAX_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1113556.1"
                     /translation="MMIAANIISMTILFAVPLLLVAMGGMFSEHSGVINIALEGMMII
                     GALFACFTLQGLDQSGFGPAHPQLSMFIAILVAGITGMIFSLFLAFAAINLKADQTIG
                     GTALNQFAPAFAVVMTWAIQGQGLTTIFIPNWVRITRETFGLAPVDGPSFWNNLIFKY
                     FYLTTPVAIVLFIAAYIVLYKTRFGLRLRACGEHPQAADSVGINVYKMRYAGVLISGL
                     LGGIGGLAYTVAAGSGFNSDVGGYGFLALAVMIFGNWKPLPILGASLFFALFKVIAAL
                     NSTLSFLPKFEGVKEISNFYQMLPYIVTMIVLIFTSKNSQAPKAEGIPYDKGSR"
     gene            3128..4450
                     /locus_tag="ACZPAX_06365"
     CDS             3128..4450
                     /locus_tag="ACZPAX_06365"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015537890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HIR1113557.1"
                     /translation="MRMYDVIAKKRDGGTLNHDELAFAVNGYVAGDVPDYQMSALLMA
                     IYLRGMTDEETAMLTDVMAHSGDMVNLSAIAGIKADKHSTGGVGDKTTLVIAPIVAAC
                     GVKIAKMSGRGLGHTGGTIDKMESVPGTRTSLTQEEFFKQVNEIGISVIGQSGKIAVA
                     DKKMYALRDVTATVGCIPLIASSIMSKKLAAGSDAILLDVTMGDGAFMKDLDGALELA
                     RQMVAIGTAHGRKVAALITDMDKPLGYNIGNSLEVAESMAVLQGKGPADLTEVCLQLA
                     SNMLVLAGKGDMPTCRKLAESVIADGTAFEKCCRMFAAQGGDISVLKDADKFRKAKYS
                     YELTAQADGYIYKNDVEKIGNASVLLGAGRIKKEDSIDFAAGITMHKKLGDYVKAGES
                     ICTFYADDESLFAAAEEMYRGGLIIRAEKPTVPPLIYARVTADGVERF"
BASE COUNT          929 a         1404 c         1340 g          958 t
ORIGIN      
        1 cccacaagct caacgagatc atggccgttt ccgaccgctg cacggtcctg cgcaagggca
       61 agtacatcgg cacggtggat atcaaggaca ccaccaaaga ggaactgtcc aagatgatgg
      121 tcggccgcga cgtgcagttt gccgtcgaga aaaagccctg cgagccgggc aagccgattc
      181 tggaagtgga aaacctcgtt gtacccagca aggtacacaa gaacaacgcc gtgcgcggcg
      241 tcagcttcaa tgtccgcgcg ggcgagatcg tctgcgtcgc cggtatcgac ggcaacggcc
      301 agagcgaact gatctacggc attaccggtc tggaaaaacc cacctccggc accatcaagc
      361 tggaaggcaa ggacatcacc agcgccccca tccgcgagcg cagcaaggcg ggtatgagcc
      421 atatcccgga ggaccgccac aagcacggcc tggtgctgga ttacacgttg gcggacaata
      481 tggttttgca gcgctattgg cagcccgagt tccagaacca cggcttcatc aaaaaggccg
      541 ccgtgcagga atacgccgag cgccttatca agcagtatga cgtgcgcagc ggccaaggcc
      601 ctgccaccat cgcccgcagt atgtccggcg gcaaccagca gaaggccatc atcgcccgcg
      661 aggtcgataa ggagccggag ctgctggtcg cagtccagcc cacccgcggc ctggacgtcg
      721 gcgcgatcga gtatatccac aagcagatcg tggccgagcg cgacaagggc cgcgcggtcc
      781 tgctcgtcag tctcgaactg gacgaggtca tgaacctgtc tgaccgcatc ctggtcattn
      841 nnnnnnnnna tttatgaagg tgagatcgtg ggcgagtttg acccgcgcaa gaccaccgtg
      901 gaagaattgg gcctgtacat ggcaggcgcc aaaaagcaag gggaggcgaa agcatgagca
      961 aacaaaataa acagccgctt ttgcagcgtc ccgccgtcgt aggcgtactg gcaagccttt
     1021 tgtccatcgt catcggtctg gtgctgggct ttgtgctgct ggtcttgctg aatccctcgg
     1081 catccctggg cggcatgaaa gccatgctga ccactggttt ttccaacatg gacaagctgg
     1141 gcaaggtgct gtatcaggca gcgccgttga tgatgtgcgg cctgtccgtc ggctttgcct
     1201 tcaagaccgg cctgttcaac atcggtgctt ccggtcagta caccatgggt gcatttttct
     1261 cgctgctgtg cgccattcag ttccagatgc cgtggtacgt ctgcctgctg gcgggcgctg
     1321 tgggcggtgc catctggggc gttttcccgg gcctgttcaa ggcgtatttc aacgtcaatg
     1381 aggtcatcac cgccatcatg ttcaactgga tcggtatgta tctggtgaac ctgctgctgg
     1441 cgaatatgcc caatatgctg gcttccggct ggggcgcgtc caactcggac cgtaccgccg
     1501 cgctgagcgc cgccaaccca ggggctatcc tgcccaaggg gccgtttgcc gcgctgttcg
     1561 gcaattccag ctggatcaac atttccatca ttatcacgat tctttttgcc atcctcgtct
     1621 gggtcatcct gcaaaagacg accttcggct atgagttgaa agcctgcggc ctcagccgtg
     1681 atgccgccca gtatgcaggc atcaattcca agcgtaacat cgtgctttcc atggtcattt
     1741 ccggtgcgct gtccggcatt ggcggtgcca tctactacct ggccggtacc ggccagtacg
     1801 tgctggaaaa gtccatcctc ggcatgggct tcaacggcat cccggtcgcc ctgctggcca
     1861 gctccaaccc catcggcacg atttttgccg cgctgttcat cagctacatt caggtcggcg
     1921 gtgccgccat gcagcctgca tactcctccg agacgatcga tatcgtcatt gccgttatca
     1981 tctacctggc ggctttcgcc ctgctgatgc gcggcgtcat tgccaaggcg gtctccggcc
     2041 gtaaagaaaa gggaggtgct gcgaaatgat gatcgctgct aacattatca gtatgacaat
     2101 cctgttcgca gtgccgctgc tgctcgtcgc tatgggcggt atgttcagtg aacactccgg
     2161 cgttatcaac atcgcgctgg aaggcatgat gatcattggc gcactgtttg cctgctttac
     2221 gctgcagggc ctggaccaga gcggtttcgg ccccgcgcac ccgcagttgt cgatgttcat
     2281 tgccattttg gtggcgggca tcaccggcat gatcttctcg ctgttcctgg cgttcgcggc
     2341 catcaacctg aaggctgacc agaccatcgg cggcacagcg ctgaaccagt ttgcccccgc
     2401 cttcgcggtc gtcatgacct gggccatcca gggccagggc ctgaccacca tcttcattcc
     2461 caactgggtg cgcatcaccc gcgagacctt cggccttgcc ccggtggacg gccctagctt
     2521 ctggaacaac ttgatcttca agtatttcta cctgacgact ccggtcgcca tcgtcctgtt
     2581 cattgcagcc tacatcgtgc tgtacaagac ccgctttggc ctgcgtctgc gcgcctgcgg
     2641 cgagcatccc caggcggcgg attccgtcgg cattaacgtc tacaagatgc gctatgcagg
     2701 tgtgctgatt tccggcctcc tgggcggcat cggcggcctg gcctacacgg tggcagccgg
     2761 ttccggcttc aactccgacg tcggcggcta cggcttcctg gcgttggccg ttatgatttt
     2821 cggcaactgg aaaccgctgc cgatcctggg cgcgtcgctg ttcttcgcgc tgttcaaggt
     2881 cattgccgca ctgaactcca ccttgagctt cctgccgaag tttgagggtg tcaaggaaat
     2941 ttccaacttc taccagatgc tgccgtatat cgtcacgatg atcgtgctga tcttcacctc
     3001 caagaactcc caggctccca aggccgaggg catcccttac gacaagggca gccgttaatc
     3061 acaacagtac gcagggccgc ccggttgaac tgccgggtgg cctctgcttt ttaaaaggag
     3121 aaaaattatg cgaatgtacg atgttatcgc caagaaacgc gacggcggta cgctgaacca
     3181 tgacgagctt gcctttgccg tcaacggcta tgtagcaggg gacgtgcccg actaccagat
     3241 gtcggcgctg ctcatggcga tttacctgcg cggcatgact gacgaggaga ccgccatgct
     3301 gaccgacgtc atggcccact ccggcgatat ggtcaatctg tccgccatcg cgggcatcaa
     3361 ggcagacaag cactccaccg gcggcgtcgg cgacaagacg acgctggtca ttgcgcccat
     3421 cgtcgcagcc tgcggtgtga agattgccaa gatgagcggc cgaggcctcg gccacaccgg
     3481 cggcacgatc gataagatgg agtctgtgcc cggcacccgc acgagcctga cccaggagga
     3541 gttcttcaag caggtcaacg agattggcat ttcggttatc ggccagagcg gtaaaattgc
     3601 tgtggccgac aaaaaaatgt acgccctgcg cgatgtgacc gccacagtcg gctgcattcc
     3661 gctgattgcc tcctccatca tgtccaaaaa gctggccgcc ggttccgacg ccattttgct
     3721 ggatgtcacg atgggcgacg gcgcgttcat gaaggatctg gacggtgccc tggagctggc
     3781 ccgccagatg gttgccatcg gcacggccca tggccgcaag gttgcggcgc tcatcaccga
     3841 tatggacaag cccctcggct acaacatcgg caactcgctg gaagtggccg agagtatggc
     3901 cgttttgcag ggcaaaggcc cggccgattt gaccgaggtc tgcctgcagc tggccagcaa
     3961 tatgctggtg ctggcgggca agggcgatat gcccacctgc cgcaagctgg ccgagagcgt
     4021 cattgccgac ggcacggcct ttgaaaaatg ctgccggatg tttgcggccc agggcggcga
     4081 tatcagcgtg ctgaaagacg ccgacaagtt ccggaaagcc aagtacagct atgagctgac
     4141 cgcccaagct gacggctata tctataagaa tgacgttgag aagatcggca acgccagcgt
     4201 gctgctgggc gcgggccgca tcaagaaaga ggacagcatc gactttgccg ccggtatcac
     4261 tatgcacaaa aagcttggcg attacgtcaa agcgggggag agcatctgca cgttctacgc
     4321 tgacgacgag agcctgtttg ccgcagccga ggaaatgtac cgcggcggcc tcatcatccg
     4381 tgccgaaaag ccgaccgtcc cgccgctgat ttacgcccgc gtgaccgcag acggtgtgga
     4441 gcggttctga ttccttaact gcccctacaa gcctgcccgg aaaagccttg taggggcggc
     4501 atatatgccg cccgcgcggc ctttacaaca aaagagcctt gcaacgcatc aacacgctgc
     4561 aaggctcttt ttatttcttc ttccgtttca gccagaacac cttgaatttg ctgtacccat
     4621 tcgccttgtc cgcgtcaaat t
//
LOCUS       DBMDSQ010000151         4585 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_4480_length_4585_cov_1.302855, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000151 DBMDSQ010000000
VERSION     DBMDSQ010000151.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 4585)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4585)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4585
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4480_length_4585_cov_1.302855"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(176..946)
                     /locus_tag="ACZPAX_06370"
     CDS             complement(176..946)
                     /locus_tag="ACZPAX_06370"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014069.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HIR1113558.1"
                     /translation="MKIDRARAQKAFADYAAHYNADDPKVKLKIDHTYRVAALCDCIA
                     KSLTLPPEDADLAWLSGMLHDVGRFEQLRRYNTFIDAQSTNHAALSASVLFDEGHIRD
                     YIDDAAEDALLRTAVAWHSAFRLPEKLDDRTRMFCQILRDADKIDILRVNVETPMEEI
                     YNVSTEALRQSPVTPAVLDAFYEHHCILHSLKKWPADNAVGHASLVFELCYPESLRIV
                     DEQGWLWQLLDFKTENPDTAKAFEAIADEMKRWIGEHM"
     gene            1115..1681
                     /locus_tag="ACZPAX_06375"
     CDS             1115..1681
                     /locus_tag="ACZPAX_06375"
                     /inference="COORDINATES: protein motif:HMM:NF025636.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR1113559.1"
                     /translation="MNAKGDANRSVRMTKQRLYQALITLLQQKSLREITVRELTELAG
                     ISRGTFYFHYADIYALMDQMEAAQLARLNDLMDSLLPGISQEEVPPALVALFTYMTEN
                     PDLCTALYGKSWESEFTRNAKKLIAERCLGEFAPGGGTEEQKYTLVFAVNGCFGTIVA
                     WQEAGVDLPPAEIARITWQAIRAVKERI"
     gene            1783..2298
                     /locus_tag="ACZPAX_06380"
     CDS             1783..2298
                     /locus_tag="ACZPAX_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HIR1113560.1"
                     /translation="MGFFDFLKKSSAPATPAEPTFPLTLAADAKGTVVAMENIPDEVF
                     AQGILGKCCGIEPTEGKVFAPVDGEITQAPDSGHALGIMGVGGVEVLIHVGVDTVEMK
                     GDGFSPKVKEGDKVKKGDLLLEMDLDKIAAAGHPAVVITVVTNTDDFKDVEVVASGDV
                     EPGADLIKVSK"
     gene            complement(2435..2593)
                     /locus_tag="ACZPAX_06385"
     CDS             complement(2435..2593)
                     /locus_tag="ACZPAX_06385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113561.1"
                     /translation="MKQNTRILCGAMAAAMTVSLAGCASNAASSTTAESSAASSSVAA
                     ASATAAAA"
     regulatory      2768..2885
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            2963..3529
                     /gene="thiT"
                     /locus_tag="ACZPAX_06390"
     CDS             2963..3529
                     /gene="thiT"
                     /locus_tag="ACZPAX_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005923196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HIR1113562.1"
                     /translation="MSKTYSKTRTLVECALMIALGTVLANIKIFEMPNGGSITLLSML
                     PFILISFRHGVKWGLFTGFVNSLLQMLLGFYAPPAPGLLPLVGMILLDYVLAFTLLGL
                     ACVIAKPFKNRLVGVAVGTAAVCGIRFLCSFLSGVLIWGNLSDGLPAWTYSLTYNGSY
                     MLPETILTTIAAVLIYKAAPQLFAEQEK"
     gene            complement(3598..4008)
                     /locus_tag="ACZPAX_06395"
     CDS             complement(3598..4008)
                     /locus_tag="ACZPAX_06395"
                     /inference="COORDINATES: protein motif:HMM:NF013412.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HIR1113563.1"
                     /translation="MKPNEEIMEFLKENPTFYLATVEGYLPRVRPIGFAMWYNDHLCI
                     GLGKHKAAYKQLLDNTNLEICAANDRGEWLRVQGTANFDNTPEAQAAAFQVMPQLADL
                     YNEETGHKLGIVYLDHISAKLFSMSGVVKDIMSY"
     gene            complement(4028..>4585)
                     /locus_tag="ACZPAX_06400"
     CDS             complement(4028..>4585)
                     /locus_tag="ACZPAX_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015537799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR1113564.1"
                     /translation="FVSEQGLARSIYEVEGAKEVAIEVCSFSKIAGFTGTRCGYTVVP
                     KALADGKLNKMWLRRQTTKFNGVAYVVQRGAEAVFTEEGMKEIQQNLDYYRANAAVIA
                     AALDEAGIWYCGGKNSPYIWLRCPNNMGSWEFFDWLLENAHVVGTPGEGFGPCGKDYF
                     RLTAFGDAERTKEAAARIKNALAAL"
BASE COUNT          916 a         1303 c         1355 g         1011 t
ORIGIN      
        1 ccgaaccccc gcagggggaa ggggtggccc ccttgttaaa gggggctgtc cgcggttaaa
       61 gggggctgtc cgcgttagcg gactggggga ttccgacggg ctttgccgca gctgctgtgg
      121 acggggagga ctgccgccct cccttgtccc tctcgttttg cccgtttgcc gtttattaca
      181 tatgctcgcc tatccaccgc ttcatctcat ccgcaatcgc ctcaaacgcc ttggcggtat
      241 caggattctc cgtcttaaaa tccagcagct gccacagcca gccttgctca tccacgatgc
      301 gcaggctctc gggatagcac agctcaaata ccagcgacgc atggcccacc gcattgtcgg
      361 cgggccattt tttcaggctg tgcaaaatgc agtggtgctc gtaaaatgcg tccagcacag
      421 cgggcgttac tgggctttgg cgcagggcct cggtgctgac gttgtagatt tcctccatcg
      481 gcgtttcaac gttgacgcgc agaatatcga ttttatcggc atcccgcaaa atctggcaga
      541 acatccgcgt gcggtcgtcc aacttttccg gcaggcggaa ggcactgtgc cacgccaccg
      601 ccgtgcgcag caaggcgtcc tcggcggcat cgtctatata gtcgcggatg tgcccctcgt
      661 caaacagcac gcttgcactc aatgcagcgt ggttggtgga ctgggcgtcg atgaaggtgt
      721 tgtaccgccg cagctgctca aagcggccga catcgtgcag cataccggaa agccaggcca
      781 gatccgcatc ctccggcggc agggtcaggc ttttggcaat gcagtcacac agcgcggcca
      841 cgcggtaggt gtggtcgatt ttcagcttga ccttcgggtc gtcggcgttg tagtgcgcgg
      901 cgtagtcagc aaaggctttc tgtgcgcggg cacggtcgat tttcataatg atcacttcct
      961 tgcctctatt gtagcatatt tgcaccgggg cgcaaattgc attgtttata aaatttcatc
     1021 aacgacagac tacatttttg tacaaaatcc ttgacaatgt ccgcgaaaac ggctattctt
     1081 aaagtagaaa tttgcgagga aggcggtgca tacgatgaac gccaaaggag acgccaaccg
     1141 cagtgtacgc atgaccaaac agcggctcta ccaggcgctt attacgctgt tgcagcagaa
     1201 aagtctgcgg gagatcacgg tccgggaact tactgagctg gccggaatca gccgcgggac
     1261 gttctatttc cactatgccg atatctatgc cctgatggat cagatggagg ctgcccagct
     1321 ggcgcggctc aatgacctga tggacagtct actgcccggc atctcgcagg aggaggttcc
     1381 ccctgccctg gtggcgctgt tcacctacat gaccgaaaac cccgacctct gcacagcgct
     1441 ttacggcaaa agctgggagt cggagtttac ccgcaacgcc aaaaagctga ttgccgagcg
     1501 ctgcttgggc gagttcgcgc cgggcggcgg caccgaggag cagaaatata cgctggtctt
     1561 tgcagtgaac ggctgctttg gtaccatcgt tgcctggcag gaggctgggg ttgacctgcc
     1621 gccggcagag atcgcccgca ttacctggca ggccatacgc gctgtaaaag agcggatttg
     1681 acccgggcaa tttctgcttg caagccttgc ctttttgggc gcggcgggat ataataaact
     1741 tacaacacac cccttgtggt gctttaagaa ggagagatta ccatgggatt ttttgatttt
     1801 ctgaaaaagt cttctgcacc ggctacccct gcggagccga cgttccccct gaccctggcg
     1861 gctgacgcca agggcaccgt tgtagcgatg gagaacatcc ccgacgaagt gtttgcacag
     1921 ggcatcctgg gcaagtgctg cggcatcgaa cccaccgagg gcaaggtctt tgccccggtc
     1981 gacggcgaaa tcacccaagc cccggacagc ggccatgcgc tgggcattat gggtgtaggc
     2041 ggcgtcgagg tgctgatcca tgtcggcgtt gacaccgtcg agatgaaggg cgacggcttc
     2101 agcccgaagg tcaaggaagg cgacaaggtc aagaagggtg acctgctgct ggaaatggat
     2161 ctggacaaga tcgcagccgc cggccacccc gccgtggtca tcacggtcgt gaccaacacc
     2221 gacgacttca aggacgttga ggtcgttgcc tccggcgatg tcgagccggg cgctgatctc
     2281 atcaaagtca gcaagtaaat tgcactacaa aaaaccgggc acctgctgtg gtgcccagtt
     2341 tttcgttgca ggggagcgag gcctgcatcc gcccctgcaa taaggttggc aacagaaaaa
     2401 tcttacgcct tctccacctc atactcgctg aacgtcacgc cgccgcagcg gtggcggaag
     2461 ctgccgccac ggaggacgac gcggcgctgg attcagccgt ggtgctgctg gcggcattgg
     2521 atgcgcagcc cgccagcgaa acggtcatgg cggcggccat tgcaccgcag aggatgcgtg
     2581 tattttgctt cataataaaa tcactcctgt tgaaataggt ggtgtgtttt ttgagtacag
     2641 ccttagtata gccgctgcaa atggcagaac cttgttgaac aggtgtgaaa ttgtgggaga
     2701 aactgtgtgt gaattattaa accgccgcgc ttgacaaacg cccgccgtcg tgtataatgg
     2761 aaactggaca taaaaacagg ggcgctgagg ggcatcctcg gctgagatcg aaacaaaccg
     2821 cagtttcggg tcgccttgaa cctgatccgg gtaatgccgg cgtaggaagt ttgacccacc
     2881 agtcataacg cttttgcgcc tgcaatgtat gttgtgggcg ctttcttgtt tttatgttac
     2941 atttttttga ggagtggaat ccatgtccaa aacctattcc aagacccgca ctctcgttga
     3001 gtgcgccctg atgatcgccc tgggcactgt gttggcgaac atcaaaattt tcgagatgcc
     3061 caacggcggc tccattacgc tgctgagtat gctgcccttt atcctgattt ccttccgcca
     3121 cggcgtgaaa tggggcctgt tcaccggctt cgtcaacagc cttttgcaga tgctgctggg
     3181 cttctacgca cccccggccc ccggcctgct gccgctggtg ggtatgattt tgctggacta
     3241 cgtgctggcc tttaccctgc tgggcctggc ctgcgtcatt gccaagccgt ttaagaaccg
     3301 cctggttggt gtggctgtcg gcacggcggc ggtgtgcggc atccgcttcc tgtgcagctt
     3361 cctgtccggc gtgctgattt ggggcaacct gagcgacggc ctgcccgcct ggacgtacag
     3421 cctgacctac aacggcagct atatgctgcc cgagaccatc ctgactacga ttgctgcggt
     3481 gctgatctac aaggcagccc cccagctgtt cgcagagcag gaaaagtaag atacgataaa
     3541 aaattcccgg cacgcaaaat cctgcgtacc gggaattttt tatacctgtt tacggtatca
     3601 ataactcatg atgtccttca cgacgccgga catggagaag agcttggcgg aaatgtggtc
     3661 aaggtagaca atgccgagct tgtggccggt ctcctcgttg tacaggtcgg ccagctgggg
     3721 catgacctgg aaagcggcgg cctgggcttc gggggtgttg tcaaagttgg cagtgccctg
     3781 cacgcgcagc cactcgccgc ggtcgttagc cgcgcagatc tccaggttgg tgttgtccag
     3841 cagctgcttg taggcggcct tgtgcttgcc caggccgatg cacaggtggt cgttgtacca
     3901 catggcaaag ccgatgggcc gcacgcgggg cagatacccc tcgacggtgg caaggtagaa
     3961 tgtaggattc tctttcagga attccatgat ctcttcattc ggtttcatgc tgatccctcc
     4021 cgcagtttta cagtgcagcc agcgcgtttt tgatgcgcgc agcggcttcc ttagtgcgct
     4081 cggcatcgcc gaacgccgtt aaacggaaat agtccttgcc gcaggggccg aagccctcgc
     4141 cgggggtgcc caccacgtgg gcgttttcca gcagccagtc aaagaactcc cagctgccca
     4201 tattgttggg gcagcgcagc cagatgtagg ggctgttctt gccgccgcag taccagatgc
     4261 ctgcctcgtc cagcgcagcc gcaatgacgg cggcgttggc gcggtagtag tcgaggttct
     4321 gctggatctc cttcatgcct tcctcggtaa agacggcctc ggcaccacgc tgtacgacgt
     4381 aggcaacgcc gttgaacttg gtggtctggc ggcgcagcca catcttgttc agcttgccgt
     4441 cggccagcgc cttgggcacg accgtgtagc cgcagcgcgt gccggtgaag cctgcaatct
     4501 tggagaagct gcagacctca atggcaacct ccttggcacc ttcgacctcg tagatactgc
     4561 gggcgaggcc ctgctcggag acaaa
//
LOCUS       DBMDSQ010000152         4573 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_4495_length_4573_cov_1.946556, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000152 DBMDSQ010000000
VERSION     DBMDSQ010000152.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 4573)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4573)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4573
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4495_length_4573_cov_1.946556"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..821)
                     /locus_tag="ACZPAX_06405"
     CDS             complement(<1..821)
                     /locus_tag="ACZPAX_06405"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HIR1113565.1"
                     /translation="MLAVTSPGFVLFAAVLAGLWFACPPPRRWQLMLGASLVFYAVLS
                     WRTLPVLLASAFLVWWCARRAAEKGIFYAGLAAALLPLALLKYLPAALGWHGTLAGAL
                     GLGYFTLQLAGYLCDVRRGRMLPEEKYLRLLCYASFFLSITQGPFNRYNKLMPQLDRP
                     TKWDTARAWRGAQRSAWGYFKKFAVADRAAVVVDAVFANPAAFDRTQLLLGGVLFTVQ
                     LYADFSGYTDIVLGLGEVLGLELPENFRQPFFADSVRDIWARWHISLSQWLRDYV"
     gene            complement(855..1949)
                     /locus_tag="ACZPAX_06410"
     CDS             complement(855..1949)
                     /locus_tag="ACZPAX_06410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113566.1"
                     /translation="MKHSFLQKAAAAALFVLLTAALLAAADFLLVDDVHSYSRVMLQE
                     LYRDAGNIDTLFLGSSHCYRSVDPAAVDAALGTHSFNAGSSQQLPDGSYYMLKEAAAQ
                     NDLKTVYLEMFYTGYNESASSNVPLACYLLVDHMNALSPNRYEYLWEMGGLAAFADLL
                     LPARHGIASLSGMPALWQAKLADGYTTDSYRYVTYEDSGEAYRGRGFVYTAGIPQDGF
                     ATLLNVDPDAPLSDFGWEYLNKITDFCQENGIQLVLFTAPLPSGYLYNTQNYQAYVDA
                     LQDFCTAHAGLEYWDFSLYRLQRDLNLKIEDFSDAHHLNGAGAEKFTAILCQTIQERA
                     AGKAVDEIFYPTVEEKLTLTPDDSILMAKM"
     gene            complement(1963..3741)
                     /locus_tag="ACZPAX_06415"
     CDS             complement(1963..3741)
                     /locus_tag="ACZPAX_06415"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIC family phosphatase"
                     /protein_id="HIR1113567.1"
                     /translation="MDCFHYPLDTETLLRKKRKLRRELLAQNPHPLHKKIAILGGSTT
                     NEVADQLGLFLLQYGIEAEFYQSEYAQYWQDAMFGTPELDDFHPDVIYIHTNWRNLTA
                     LPTTADSEADIDAMLDAQYAHFETMWQALERKFACPVIQNNFDRPNYRLMGNRDIWDP
                     HGRSNFISRLNQKFYAYAASHEHFYINDIDYLSADYGLTAWGDAFFWHMYKYAMCLDA
                     IPSLANSVANIIKSLYGRNKKALVLDLDNTLWGGIVGDDGVDGLAIGPEVPEGQVYAE
                     FQSYCKALKSIGVILAVDSKNDEANALAGLNHPDGVLRPDDFVAIKANWEPKDLNLKA
                     IASELNLGADSFVFADDNPAERAIVAAQVPGVAVPALDGAENYIKTLDHGGYFEVTAL
                     SKEDLKKTELYHQNAERAKAQAAFTDYGQYLDSLEMTATVKGFEPLYIQRIAQLTNKS
                     NQFNLTTLRCSEDDIHAMAENAAYISLCGKLVDKFGDNGIVTVVAGQQQGGALHIRLW
                     LMSCRVLKRGMEDAMMDTLVAEAAARGIKTIVGYYYPTAKNAMVREFYAQFGFAKTAE
                     DADGNTTWTLDVAAYAPKHPHMKIER"
     gene            complement(3975..4232)
                     /locus_tag="ACZPAX_06420"
     CDS             complement(3975..4232)
                     /locus_tag="ACZPAX_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03905 family TSCPD domain-containing
                     protein"
                     /protein_id="HIR1113568.1"
                     /translation="MTYTYNNKGTCSVRTVVELNDDHTIKSVQVLGGCDGNLKGIAKL
                     LPGMKAEDAIARMEGTTCGRKPTSCPDQIAQALKGALAEMQ"
     gene            complement(4264..>4573)
                     /locus_tag="ACZPAX_06425"
     CDS             complement(4264..>4573)
                     /locus_tag="ACZPAX_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HIR1113569.1"
                     /translation="SRAVVVLAHPSVYHSMELARELIAAGRLDGVEINHPRNTPEDKA
                     ELARLAKENGLIVTGGTDYHGINTVTPRPVGAFSTSDEMIARIGDIAKARKATWKKAK
                     "
BASE COUNT          883 a         1316 c         1425 g          949 t
ORIGIN      
        1 tagacgtaat cccgcagcca ttgggacagg ctgatatgcc accgcgccca gatatccctg
       61 acggaatccg caaagaaggg ctggcggaag ttttcgggca gctccagccc cagcacctcg
      121 ccgaggccaa ggacaatgtc cgtgtagccg gagaagtccg cgtagagctg cacggtgaac
      181 agcacaccac ccagcaaaag ctgggtgcgg tcaaaggcgg cggggttggc gaacacggcg
      241 tccaccacca cggcggcgcg atccgccacc gcaaactttt taaagtagcc ccacgcgctg
      301 cgctgcgcgc cgcgccacgc ccgggcggta tcccattttg tggggcggtc cagctggggc
      361 atgagcttgt tgtagcggtt gaaaggtccc tgggtgatag acaaaaagaa cgacgcatag
      421 cacaaaagcc gcaggtattt ttcttccggc agcatacgtc cccggcgcac atcgcagagg
      481 tagcccgcca gctgcagggt gaagtaccca agccccagcg cccccgccaa ggtaccatgc
      541 cagcccaggg cggcgggcag gtacttgagc aaggccagcg gcagcagcgc ggcggccagc
      601 cccgcgtaaa agatgccttt ttccgccgcg cgacgggcgc accaccaaac cagaaacgcg
      661 cttgccagca gcaccggcag cgtccgccag gagagcacgg cgtaaaatac caggctggcc
      721 cccagcatca actgccagcg gcgcggcggc gggcaggcaa accaaagccc cgccagcacc
      781 gctgcgaaca gcacaaaacc gggtgatgtc acagcgagca tagtggggcc tccttgtata
      841 gattattgtt gcgattacat ttttgccata aggatggaat catccggcgt tagggtcagt
      901 ttttcttcca ccgtggggta gaaaatttcg tccacggcct tccctgccgc gcgttcctga
      961 atcgtttggc agaggatggc agtgaacttt tccgcgcctg caccgttcag gtggtgcgca
     1021 tcggagaagt cctcaatttt caaattgaga tcgcgctgca ggcggtagag ggagaaatcc
     1081 cagtattcca ggcccgcgtg cgcggtgcaa aaatcctgca gggcatccac ataggcttgg
     1141 tagttttgcg tgttgtacag atagccgctg ggcagcggag ccgtgaacag cacgagttgg
     1201 atgccgtttt cttgacaaaa atcggtaatt ttgttcaggt attcccagcc aaagtcagag
     1261 agcggcgcgt cagggtcgac attcagcagc gtggcaaagc cgtcctgcgg gatacccgcg
     1321 gtgtagacga agccccggcc gcggtaggcc tcaccgctgt cctcgtaggt tacatagcgg
     1381 tagctgtccg tcgtgtagcc gtcggccagc ttggcctgcc acagcgcagg catcccggac
     1441 agcgacgcta tgccatggcg ggcgggcagc agcagatcgg caaaagccgc caagccgccc
     1501 atctcccaca ggtactcata acggttgggc gaaagggcgt tcatgtggtc gaccagcaag
     1561 tagcaggcca gcggcacatt gctggatgca ctttcgttgt aaccggtgta gaacatttca
     1621 agatatacgg tctttaggtc gttctgcgca gcggcttctt tgagcatata gtaggagccg
     1681 tccggcagct gctgggacga cccggcgtta aagctgtggg tgcccagcgc ggcgtccacg
     1741 gcggcgggat ccacgctgcg gtaacagtgg gacgaaccaa ggaacagtgt atcgatattg
     1801 cccgcatccc ggtacaattc ttgcagcatc acacgggagt aggagtgtac gtcatccacc
     1861 agcagaaaat ccgcagccgc cagcagtgcc gccgtaagca gcacaaacag cgcggcggcc
     1921 gccgcttttt gcaggaagga gtgtttcata gcgtttttcc tatcagcgtt caattttcat
     1981 gtgggggtgc ttcggcgcgt aggcggccac atccagcgtc caggtcgtat tgccgtcagc
     2041 atcctcggcg gtcttggcaa agccgaattg agcgtaaaat tcacgcacca tagcgttttt
     2101 agccgtggga tagtagtagc ccacaatggt cttgatgccg cgggcggcgg cctcggctac
     2161 gagagtgtcc atcatagcat cctccatgcc gcgcttgagc acgcggcagc tcatgagcca
     2221 caggcgtata tgcagtgcgc cgccctgctg ctggcccgcc acaacggtga cgatgccgtt
     2281 atcgccgaac ttatccacga gcttaccgca caggctgata taggcagcat tctcggccat
     2341 agcgtggata tcatcctcgg aacaacgcag cgtcgtcaaa ttgaactggt tggacttgtt
     2401 ggtgagctgg gcaatgcgct ggatgtacag tggttcaaag cccttgaccg tggcggtcat
     2461 ttccaggctg tcgagatact ggccgtagtc ggtgaaagcc gcctgggcct tggcgcgctc
     2521 ggcgttctgg tggtagagtt cggtcttttt caaatcctct ttcgagaggg ccgtgacctc
     2581 aaagtagccg ccgtgatcca gcgttttgat gtagttttcg gcgccgtcca gcgcgggcac
     2641 tgccacgccg ggcacctggg cggccacgat ggcgcgctct gccgggttgt catcggcaaa
     2701 aacaaagcta tccgcgccga ggttcagctc ggacgcgatg gccttgaggt tcaagtcctt
     2761 tggctcccag ttggccttga tggcgacaaa atcgtcgggg cgcagcacgc cgtccgggtg
     2821 gttcaggccc gccagcgcgt tggcctcgtc gtttttggag tcgacagcca ggataacgcc
     2881 gatggatttc agcgccttgc agtagctttg gaactcggcg tagacctggc cttcgggcac
     2941 ttcggggccg atggcaaggc cgtccacacc gtcgtcgccg acgatgccgc cccagagcgt
     3001 attgtccagg tcgagcacaa gcgctttttt gttgcggccg tacaaggact tgatgatatt
     3061 ggccacgctg ttggccagcg aagggatagc gtccaggcac atggcatatt tgtacatatg
     3121 ccagaagaag gcgtcgcccc aggcggtcag gccgtagtca gcggaaagat agtcgatatc
     3181 gttgatatag aaatgttcat gcgatgcggc gtaggcgtag aatttctggt tcagacggct
     3241 gatgaaattg gagcggccgt ggggatccca gatatcgcgg ttgcccatga gtcgataatt
     3301 aggcctatca aagttattct ggatgaccgg gcaggcgaat tttcgttcca gcgcctgcca
     3361 cattgtctca aaatgggcat actgcgcgtc cagcatggca tcaatatcgg cttcgctgtc
     3421 ggcggtggtg ggcaaggcgg tcaggttgcg ccagttggtg tggatgtata tgacatccgg
     3481 gtggaagtcg tccagctcgg gcgtaccgaa catggcgtcc tgccagtact gggcgtactc
     3541 gctctggtaa aactctgcct cgatgccgta ttgcaacaga aacagcccca gctggtcggc
     3601 cacttcgttg gtggtcgaac cgccgaggat ggcgattttt ttgtgcagcg ggtgggggtt
     3661 ctgggccagc agctcacgcc gcagcttgcg ctttttccgc agcagggtct cggtatcgag
     3721 agggtaatgg aagcagtcca tgggaagctc ctttcaacgt caaaaggctt ccccatcggg
     3781 ggaagctgtc tgcgaagcag actgatgagg gcaaagcctg ccggcaccac ctgtctgcgg
     3841 gggatgccac tgaaaagcct gccctcatcc ggcctcgcca gctcggccac cttcagtctt
     3901 cgcgctaaga gccgcctgcg gcggttgcgc tccgacacgc acctgcgggt gcagccccgc
     3961 ggggaaaagg ctttttactg catctccgcc agtgcgccct tcaatgcctg ggcgatctgg
     4021 tcggggcagc tggtgggctt gcggccgcag gtcgtgcctt ccatgcgggc gatggcgtcc
     4081 tcggccttca tgccgggcag cagcttggcg atgcccttca ggttgccgtc gcagccgcca
     4141 agaacctgca cgctcttgat ggtgtggtcg tcgttcagtt ccacaacggt gcggacggaa
     4201 caggtgcctt tgttgttgta cgtgtaagtc atgatgtatt gctccttaaa ttttattgct
     4261 cttttatttt gcctttttcc aggttgcctt gcgcgccttg gcaatgtcgc cgatgcgtgc
     4321 aatcatttcg tcactggtgg agaatgcccc caccgggcgc ggggtcacgg tgttgatgcc
     4381 gtggtagtcc gtgccgccgg taacgatcaa tccgttttct tttgccaggc gggcaagctc
     4441 ggccttgtcc tccggcgtgt tgcgggggtg gttgatctcg acgccgtcca gccgcccggc
     4501 tgcgatcagc tcccgggcca gctccatgct gtggtagacg ctggggtggg ccagcacgac
     4561 cactgcgcgg ctt
//
LOCUS       DBMDSQ010000153         4566 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_4503_length_4566_cov_1.855767, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000153 DBMDSQ010000000
VERSION     DBMDSQ010000153.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 4566)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4566)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4566
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4503_length_4566_cov_1.855767"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..2301)
                     /locus_tag="ACZPAX_06430"
     CDS             complement(<1..2301)
                     /locus_tag="ACZPAX_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HIR1113570.1"
                     /translation="MPSITGLTAAEVNARRAAGQTNTPPKSQTKTTGEIVRDNICTYF
                     NLVFLVLAVMLALVRSWLNMGFLGIVFCNTFIGIVQQLRAKRTIDKLTLVSAQKIRCL
                     RDGRWCEVLSDDLVQDDVVEFGAGDQIVADAVVLDGSAQANESLITGEARAVPKECGA
                     ELKSGSFLMAGRCVARLTRVGAESYASRLTAEAQANGHKVARGEMMRSLDKLIKFIGI
                     ALVPIGAVLIWKQHWVLELPMKDTVDATVAALIGMIPEGLYLLTSVALAVSMMRLARR
                     KVLTRDMNCIETLARVDTLCVDKTGTITESAMQADDPLPLAENEPLDAILASFYAGEQ
                     PDNDTGRALAARFGQGGTGWFARCSVPFNTAYKYSAKDFGAQGCYVVGAPDVLAGVRA
                     GEFADKLAPLLAQGRRVLLLAKYNAALPDPPAALDPAQLEFLALLPLQNRIRENAPKT
                     FRYFAKQGVAVKVISGDDPQAVSHVAANAGIAGAERWVDAATLRSEQALAETAEKCTV
                     FGRVTPEQKRQLVHALQAKGHTVAMTGDGVNDVLALKDADCGIAMASGAQAASQVAQL
                     VLLDSDFAALPSVVGEGRRVINNIQRSASLFLVKNIFSFLLSIVALALPLAYPFQPLQ
                     LSLVTFATIGAPAFFLALEPNHELVRGKFMRNVLRAALPGGLTDFLLVFCTQGFGYAF
                     DIPTDMVGTICTFVILCVGLQILWGVCIPFTPLHWIIWGGMTVMGVAGAFLLAPWLPL
                     VRLDLGGMLVLVVLLALSAPTLAGLTF"
     gene            complement(2543..3010)
                     /locus_tag="ACZPAX_06435"
     CDS             complement(2543..3010)
                     /locus_tag="ACZPAX_06435"
                     /inference="COORDINATES: protein motif:HMM:NF020897.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIR1113571.1"
                     /translation="MQDRFIRLSVSLARLQKVIQRIKTSGMGRIALKAGHTLVLCQLA
                     AAPEGWHFSDLTERCDLDPAMISRVLADLVRSGLVEKCGAPGKYNALYLLTDAGRDRA
                     ARVTAVVRDVERRADEGIDPADLATFYKVLNQLTKNLEAVAADPAEAFEPLER"
     gene            complement(3012..4133)
                     /gene="prfB"
                     /locus_tag="ACZPAX_06440"
     CDS             complement(3012..4133)
                     /gene="prfB"
                     /locus_tag="ACZPAX_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HIR1113572.1"
                     /translation="MIEFDEYKVKLNNIRPRLDALADSLGIEAAKEEIDRLHAQIDSD
                     GFWDNQEISQKVMKQSRTLEAKVARYEKMCSQWDDLYTLCEMALEDNDDSMLPELTEG
                     YAQLEQEMENARLETLLSGEYDNNNAIVSFQAGAGGTEAQDWASMLYRMYTHWTEQHG
                     LTNKILDYLDGDEAGIKSASILIEGENAYGMLKSEHGVHRLVRISPFDANARRQTSFS
                     SLEVMPELDNNINIEINPADIEMQVYRSSGAGGQHINKTSSAVRLIHKPTGIVTSCQT
                     QRSQLQNREYAMEMMKAKLYQIALQQHKDKIDDIKGVQNEIAWGHQIRSYVFMPYTLV
                     KDNRTGYESSNVQAVMDGDLDGFIFAYLKAASRGELKED"
     gene            complement(4221..>4566)
                     /locus_tag="ACZPAX_06445"
     CDS             complement(4221..>4566)
                     /locus_tag="ACZPAX_06445"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013702.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HIR1113573.1"
                     /translation="ILKAIQFIKDDKASIAVFPEGGTNKTDAPLLPYRSGVFKIAQKS
                     NVPIVVCSLVNSRAILHNMFRKHTEVWLDVLDVVPAEELDGKTAIEVGERVHAVMEAG
                     IVARETEQGLRA"
BASE COUNT          853 a         1425 c         1386 g          902 t
ORIGIN      
        1 aaaggtcagc cccgccaggg tcggggcgct gagggccagc aatacgacca agaccagcat
       61 gccgcccaag tccagccgca ccagcggcag ccagggggcc agcaggaacg cccccgcgac
      121 ccccatcacc gtcatgccgc cccagatgat ccagtgcagc ggcgtgaacg gaatgcagac
      181 accccacaag atctgcaagc ctacgcacaa aatcacgaag gtgcagatcg tgccaaccat
      241 gtctgtgggg atatcaaacg catagccaaa tccctgggtg cagaacacga gcaggaagtc
      301 tgtcagcccg ccgggcagcg cggcccgcag gacgttgcgc atgaatttcc cgcgcaccag
      361 ctcgtggttt ggctccaatg ccaggaaaaa cgccggtgcg ccgatggtgg caaatgtgac
      421 gagggacagc tgcaaaggct ggaacggata ggccagcggc agcgccaatg ccacgatgct
      481 gagcaggaac gagaagatat ttttgaccag aaacagcgac gctgaccgct ggatgttgtt
      541 gatgacacgc cgcccctcgc ccacaacgct gggcagtgcg gcaaagtcag aatccaacag
      601 caccagctgc gccacctggc tggcggcctg tgcaccggac gccatggcga tgccgcagtc
      661 ggcatctttt aacgctaata cgtcatttac tccatctccg gtcatggcaa ccgtgtgtcc
      721 tttcgcctgc aaggcgtgga cgagctggcg cttctgctcg ggcgtcacac ggccaaagac
      781 cgtgcatttt tcggcggtct cggccagcgc ctgctcactg cgcagcgtcg cggcgtccac
      841 ccagcgctcg gcccccgcaa tgcctgcgtt ggcggccaca tggctgacgg cctgcgggtc
      901 atcgcccgaa atgaccttga ccgccacacc ctgcttggca aagtagcgga aggtcttggg
      961 cgcgttttcc cggatgcggt tttgcagcgg cagcagcgcc agaaattcca gctgcgcggg
     1021 atcgagcgcg gctggcgggt cgggcagtgc ggcgttatat tttgccagca gcagcacgcg
     1081 gcggccctgg gccagcagcg gtgccagctt gtcggcaaac tcccctgccc gcaccccggc
     1141 cagcacatcc ggcgcaccga cgacgtagca gccctgcgcg ccaaaatcct tggcgctgta
     1201 cttgtacgcc gtgttgaagg gcacgctgca ccgcgcaaac cagccggtgc cgccctgccc
     1261 gaagcgagcc gccagagcac ggccggtgtc attgtcgggc tgctcacccg cgtaaaagct
     1321 cgccaagatc gcgtccagcg gttcattttc cgccagtgga agcggatcgt cggcctgcat
     1381 ggcgctttcg gtgatcgtgc cggtcttgtc cacacagaga gtgtccaccc gggccagcgt
     1441 ctcaatgcag ttcatatcgc gggtcagcac cttgcggcgg gccaggcgca tcatgctcac
     1501 agccaaagct acactggtca gcaggtacag cccctcgggg atcatgccga tgagcgccgc
     1561 gaccgtcgcg tccacggtgt ccttcatcgg cagttccagc acccaatgct gcttccagat
     1621 cagcacagcg ccgatgggaa ccaacgcgat accgatgaat tttatcagct tatccaggct
     1681 gcgcatcatt tcgccccggg cgactttgtg accgttggcc tgggcctcgg cggtcaggcg
     1741 gctggcgtag ctctcggccc cgacgcgggt cagccgggcg acacagcgcc ccgccattaa
     1801 aaagctgcca gatttcagct ctgcgccgca ctccttcggc acggcccggg cctcgccggt
     1861 gatgagggat tcgttcgcct gggcactgcc gtccagcaca acagcgtcgg ccacgatctg
     1921 gtcgcccgcg ccaaactcga ccacatcgtc ctgcacaagg tcgtcggaca gcacctcgca
     1981 ccaccggccg tcccgcaggc agcgaatttt ctgcgccgag acaagggtca atttgtcgat
     2041 ggtacgttta gcgcgcagct gctgcacgat gccgatgaag gtattgcaaa acacgatgcc
     2101 aaggaacccc atgttcagcc aggagcgcac gagcgccagc ataacggcca gcacaaggaa
     2161 cacaaggttg aagtaggtgc agatattgtc ccgcacgatc tcgccggtgg tcttggtctg
     2221 ggacttgggc ggtgtgttcg tctgccctgc cgcgcggcgt gcgttgacct cggcagcggt
     2281 cagcccggtg atggatggca tgagagggtg gctcctttca ggtggtagag gttgtagggg
     2341 ccgggtatgc ccggcccggt cgcagaaggc aaacgaccgt ttatgggaaa gctgcgggcc
     2401 gcacctgtgc ggcccctaca atagtaaaac gaactacatc agcttattat ggcaagtata
     2461 cccgccaagt atgaacaaaa caagtgcccg gctgcgtttt ggggcagtcg ggcatttttg
     2521 tctatatgcg gttgtcgcgg ttttatcttt ccaacggttc aaacgcctct gccgggtcgg
     2581 cggccacggc ttccaggttc tttgtcagct ggttcagcac cttataaaac gtcgccaggt
     2641 cggcaggatc tatcccctcg tcggcgcggc gctccacgtc acgcaccacg gcggtcacac
     2701 gggcggcgcg gtcgcggccc gcgtcggtca acagatacag cgcgttgtac ttgccgggcg
     2761 caccgcactt ttccaccagg ccgctgcgga ccaaatccgc cagcacgcgg gagatcatgg
     2821 ccgggtccag gtcgcaccgc tcagtcaggt cggagaaatg ccagccctcg ggggcggctg
     2881 ccagctggca gagcaccagc gtgtggcccg ctttcagcgc aatgcggccc ataccgctgg
     2941 tcttgatgcg ctggatgacc ttttgcagcc gcgccagcga gacggacagt cggatgaaac
     3001 ggtcctgcat atcaatcttc cttcagctcg ccgcgggagg ccgccttcag gtacgcaaag
     3061 atgaagccgt ccaggtcgcc atccatgacg gcctgtacgt tgctgctctc gtagccggtg
     3121 cggttgtcct tgaccagcgt gtagggcatg aacacatagc tgcggatctg gtggccccag
     3181 gcaatctcgt tctgcacgcc cttgatgtcg tcgattttgt ccttgtgctg ctgcaaagcg
     3241 atctggtaca gcttggcctt catcatttcc atggcgtact cacggttctg cagctggctg
     3301 cgctgggtct ggcagctggt cacaatgccg gtgggcttgt ggatcaggcg cacggccgag
     3361 gacgtcttgt tgatgtgctg gccgcccgcg ccggaggaac ggtagacctg catctcgata
     3421 tcggcggggt tgatttcgat attgatgttg ttgtccaact caggcatgac ctccaggctg
     3481 gagaagcttg tctggcggcg ggcgttggcg tcaaacgggc tgatgcgcac caggcggtgg
     3541 acgccgtgct cgcttttgag cataccgtag gcgttctcac cctcgatgag aatgctggcg
     3601 gatttgatgc ccgcctcgtc gccgtcgaga tagtcaagga tcttgttggt caggccatgc
     3661 tgctcggtcc agtgcgtgta catccggtag agcatcgagg cccagtcctg cgcctcggtg
     3721 ccgcctgcgc ccgcctggaa cgagacgatg gcgttgttat tgtcatattc gccggaaaga
     3781 agcgtctcaa gccgtgcgtt ttccatctcc tgctcgagct gggcatagcc ctcggtcaac
     3841 tcggggagca tggagtcgtc gttgtcctcc agcgccattt cgcagagcgt atacagatcg
     3901 tcccactggc tgcacatttt ttcgtatcgc gcgaccttgg cctccagcgt ccggctctgc
     3961 ttcatgacct tctgcgagat ctcctgatta tcccagaagc catcggaatc gatctgcgcg
     4021 tgcaggcggt cgatttcctc tttcgcggcc tcaatgccga gggagtccgc cagcgcgtcc
     4081 agcctggggc ggatattatt gagctttacc ttatattcat cgaattcaat catgcttttc
     4141 ccacactttt caaaattgtc gggatatatt atacaggaaa accggcggga tgtaaagggg
     4201 aaacgagaat tctggacgct ttatgcgcgc agcccctgct ccgtctcacg ggcgacgatc
     4261 ccggcctcca tgaccgcgtg gacgcgttcg ccgacctcaa tggccgtttt tccgtccagc
     4321 tcctccgcag gaacgacgtc cagcacgtcc agccagacct ccgtgtgctt gcggaacata
     4381 ttgtgcagaa ttgcgcggga attgaccagc gagcagacca cgatgggcac attcgacttc
     4441 tgcgcgatct tgaacacgcc gctgcggtag gggagaagcg gagcgtccgt tttgttcgtt
     4501 ccgccctccg ggaatacggc gatggaggcc ttgtcgtcct tgatgaactg gatggccttg
     4561 agaata
//
LOCUS       DBMDSQ010000154         4525 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_4543_length_4525_cov_1.694168, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000154 DBMDSQ010000000
VERSION     DBMDSQ010000154.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 4525)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4525)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4525
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4543_length_4525_cov_1.694168"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            104..532
                     /locus_tag="ACZPAX_06450"
     CDS             104..532
                     /locus_tag="ACZPAX_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HIR1113574.1"
                     /translation="MAERRFLLVDAEVLPEVFVKVLQAKELLASGAVSNISAAAKQAG
                     ISRSAFYKYKDTVFDAETGRESITVVATLLDKTGALQSLLSGISAAGASIVTITQSRP
                     ENGAAQVEVTVRTGTMQMPVDEMLTRLSHQPCVVEIHRGA"
     gene            620..1849
                     /locus_tag="ACZPAX_06455"
     CDS             620..1849
                     /locus_tag="ACZPAX_06455"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HIR1113575.1"
                     /translation="MAKIAIMGFGTVGSGVLEVCRRNAASIARRVGEPVEVKYILDVR
                     DFSASPDAALFTNSIDTILNDPEIKVVVETIGGTRFAYPYVRQCLESGRSVCTSNKEM
                     VATYGAELLALAKAHNAAFLFEASVGGGTPIITPMHQCLAANHISQIRGIVNGTTNFM
                     LTKMKRENMGFDAALKIAQQLGYAETKDPGDDVDGRDACRKIAILASLACGHHVYPDN
                     IPARGIRDITVADIKAAEKLDSAIKLIAWYDEGEDGNMAAGVEPMLVSNANQLAGVDD
                     VFNAVLMKGDMLGDVVFYGKGAGKLPTASAVVADVIDALKEGVKIHDSLFWKPAEKPE
                     GQLEDKNSYPWYIRVTGVAAALLPSVAGAGHTVFEENGEAAYLIDKATAADVAEITKK
                     IEVLGGKVVLAMKKLEE"
     gene            1865..>1993
                     /locus_tag="ACZPAX_06460"
     CDS             1865..>1993
                     /locus_tag="ACZPAX_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HIR1113576.1"
                     /translation="MVTVTVPATSANVGAGFDSLGLAVSMHNVFTFEESDRIQISSV"
     assembly_gap    1994..2003
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2004..2760
                     /gene="thrB"
                     /locus_tag="ACZPAX_06465"
     CDS             <2004..2760
                     /gene="thrB"
                     /locus_tag="ACZPAX_06465"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002847587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HIR1113577.1"
                     /translation="THVPAGSNNLVYRSARVVYDQLGIPLKGLRITQRNDIPMARGLG
                     SSSACIVAGIMGANALLGDKLTERQMLTLATAIEGHPDNVAPAMLGGFVTSVIDEGQV
                     YSVKKDIDPELAFAAFVPDFRLLTSKARAALPAMVSHKDAVYNLSRAALATAAFCEGN
                     YALLGVATKDVLHQKYRLPLIEGGDDIFELAQDLGAQAVYISGAGPTIMAVVHKDDTE
                     FFTRAEAALAADGPLHHFTVHRLLADNVGAVVS"
     gene            2784..3989
                     /locus_tag="ACZPAX_06470"
     CDS             2784..3989
                     /locus_tag="ACZPAX_06470"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HIR1113578.1"
                     /translation="MGLIVQKFGGSSVKDRDRIFNVARIVMNTRNAGHDVVVVVSAQG
                     DTTDDLITKAAEITSDPSHREMDMLLAAGEEISISLLTMALQELGVSAISLTGWQAGF
                     TTDRAYSKARIKRLDTERVESELARNRVVVVAGFQGLNRSDDITTLGRGGSDTSAVAL
                     AAALHADRCQIFTDVEGVYTADPRKIPKATKLKEITFDEMLELASLGAQVLNNRSVEL
                     AKKYNVELEVISSINPVPGTIVKEETKVEGMLIKGVAKDTDVAVLTVKDVPDVPGMSF
                     KIFSLLAQKNINVDIILQTTGRDGKKDMSFTVPLGDAENAVSALKNATSRIGGGDITV
                     DKTCAKVSIVGAGMQSHSGVASTMFEALFNQNINIKMISTSEIKISVIIDEADADKAV
                     AAIHDAFIN"
     gene            complement(4071..>4525)
                     /locus_tag="ACZPAX_06475"
     CDS             complement(4071..>4525)
                     /locus_tag="ACZPAX_06475"
                     /inference="COORDINATES: protein motif:HMM:NF014081.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR1113579.1"
                     /translation="KRNRVMIAHCPTSNENVIAGIAPAAKYLHGGWRIGLGSDVAGGH
                     TLDLFAVMAAAVQVSKLRWRYIDQSEKPLTMTEALYMATVGGGDFWADFGENVGLFED
                     GYAFDALVLDDSALKNMRTFTPTERLERYAYLGRGALTAKFAQGKKLL"
BASE COUNT          941 a         1381 c         1373 g          820 t
ORIGIN      
        1 tgacaaattg cacaaaaacc gctcaaaaag gcttgcaaca ggccccgggc cgtggtatca
       61 tgggacacat cgagaaacat ttgtacacaa gggggaaaca ccaatggccg aaaggcgctt
      121 tctgctggta gatgcagagg ttctgccgga agtttttgtc aaggtgctgc aagccaagga
      181 actgctggcc tccggcgcgg tgagcaacat ctccgccgcc gccaagcagg caggcatttc
      241 gcgcagcgca ttctataaat ataaggatac cgttttcgat gccgagaccg gccgcgagtc
      301 catcacggtg gtggcaacgc tgctggataa gaccggcgca ctgcaaagcc tgctgtccgg
      361 tatttcggcg gcgggcgcgt ccatcgtgac catcacccag tcccgcccgg agaacggcgc
      421 ggcccaggtg gaggtcacgg tccgcaccgg cacgatgcag atgcctgtgg atgagatgct
      481 gacccgcctg tcccaccagc cctgcgtggt ggagattcac cgcggggcgt aacttctctt
      541 ggccgcaggg taagcccggc ccctacaagg cggcttcacg gacagatacc gagtgtaaga
      601 atacaggggg aaaattccaa tggctaaaat tgctatcatg ggcttcggca cggtcggttc
      661 cggcgtgctg gaggtttgcc gccgcaacgc ggcatccatc gcccgccgcg tcggtgagcc
      721 ggtcgaggtc aaatacatcc tggatgtgcg ggatttctcc gcctccccgg acgccgcact
      781 ttttaccaac tccatcgaca cgattttgaa cgaccccgag atcaaagtcg tggtcgagac
      841 catcggcggc accagattcg cctatccgta tgtgcgccag tgtttggaga gcggacgcag
      901 cgtctgcact tctaataagg aaatggtcgc tacctacggc gcggagctgc tggcccttgc
      961 caaagcgcac aacgcggcct tcctgtttga ggccagtgtc ggcggcggca cgcccatcat
     1021 caccccgatg caccagtgcc tggccgccaa ccacatcagc cagatccgcg gcatcgtcaa
     1081 cggcaccacg aacttcatgc tgaccaagat gaagcgggag aacatgggct ttgacgctgc
     1141 gctcaaaatc gcccagcagc tcggctatgc cgagacgaag gatcccggcg acgatgtgga
     1201 cggccgcgat gcctgccgca aaattgcgat tctcgccagc ctggcctgcg ggcatcacgt
     1261 ttacccggat aacatcccgg cccgcggcat ccgcgacatc acggtcgccg atatcaaggc
     1321 cgctgaaaag ctggacagcg ccatcaagct gatcgcctgg tacgacgagg gcgaggatgg
     1381 aaacatggcc gccggcgtgg agccgatgct cgtctccaac gcgaaccagc tcgcgggcgt
     1441 ggacgatgtg ttcaatgctg tgctgatgaa gggcgatatg ctgggcgatg tcgtgttcta
     1501 cggcaagggc gcgggcaagc tgcccaccgc cagcgccgtg gtggcggatg tcatcgatgc
     1561 actgaaagag ggcgtcaaga tccacgactc gctgttttgg aaacccgccg aaaagcccga
     1621 gggccagctg gaggacaaga acagctaccc ttggtacatc cgtgtgaccg gcgttgcagc
     1681 ggcgctgctg cccagtgtgg ccggtgcggg ccacacggtc tttgaggaaa acggcgaggc
     1741 cgcttacctc atcgacaagg ccactgccgc cgatgtggcc gagatcacca agaaaattga
     1801 ggtgctgggc ggcaaggtcg tcctggccat gaaaaaactg gaagagtaag aggggacagg
     1861 gcatatggta acagtaacgg tcccggccac cagcgccaat gtcggcgcag ggtttgactc
     1921 gctggggttg gccgtctcga tgcacaacgt tttcacgttt gaggaatccg accgcatcca
     1981 gatctcgtcc gtcnnnnnnn nnncacccac gtcccggcgg gcagcaacaa cctggtctac
     2041 cgctcggccc gcgtcgtcta tgaccagctg ggcatcccgc tgaaaggtct gcgcatcacc
     2101 cagcgcaacg atatccccat ggcccgcggt ttgggatcca gttcggcctg catcgtggcg
     2161 ggcatcatgg gcgccaacgc cctgctgggc gataaactga ccgagcgcca gatgctgacg
     2221 ctggcaacgg ccattgaggg ccaccccgac aacgttgcgc ccgccatgct gggcggcttt
     2281 gtcaccagcg ttatcgacga aggccaggta tacagcgtca aaaaggatat cgacccggaa
     2341 ctcgcctttg cggcgttcgt gccagacttc cgcctgttga ccagcaaagc gcgtgcggcg
     2401 ctgcctgcca tggtcagcca taaggacgcg gtctacaatt tgtcccgcgc ggcgctggca
     2461 acagcggctt tctgcgaggg caactacgcg ctgctgggcg tggcgaccaa ggacgtactg
     2521 caccagaaat accgcctgcc gcttatcgaa ggcggcgacg atatctttga attggcgcag
     2581 gatttgggcg cacaggctgt gtatatctcg ggcgcagggc ctaccatcat ggccgttgtc
     2641 cacaaggacg acaccgagtt tttcacccgc gccgaggcgg cgctggcggc ggatggcccg
     2701 ctgcatcatt ttaccgtgca tcgtctgctg gccgacaatg tcggcgcagt agtcagttaa
     2761 gataggggag agggataaca ccaatgggct tgatcgtaca aaaattcggc ggctcctcgg
     2821 tcaaagaccg tgaccgcatc ttcaacgtgg cgcgcatcgt catgaacacc cgcaacgccg
     2881 gccacgacgt tgttgtggtc gtttcggcgc agggcgacac caccgacgac ctgatcacca
     2941 aggcggcgga aattacgtcc gacccgtccc accgcgagat ggatatgctg ctggccgcag
     3001 gcgaggaaat ttcgatttcc ctgctgacga tggccttgca ggaacttggc gtctccgcca
     3061 tcagcctgac cggttggcag gcgggcttca ccaccgaccg cgcctactcc aaggcccgca
     3121 tcaagcggct ggacaccgag cgtgtcgaga gtgagcttgc ccgcaaccgc gtcgtcgtcg
     3181 tggcgggctt ccagggcttg aaccgctctg acgatatcac gacactgggc cgcggcggca
     3241 gcgatacctc tgccgtggca ctggcggctg ccctgcatgc agaccgctgc cagatcttca
     3301 ccgacgtcga aggtgtctac actgccgacc cgcgcaagat ccccaaggcc accaagctga
     3361 aagagatcac gtttgacgaa atgctcgagc tggcgtccct gggcgcacag gtgctgaaca
     3421 accgcagtgt cgagctggcc aaaaagtaca atgtcgagct ggaagtcatt tccagcatca
     3481 atccggtgcc cggcaccatc gttaaggagg aaaccaaagt ggaaggtatg ctcatcaagg
     3541 gtgttgcaaa ggacaccgac gtcgccgttt tgaccgtcaa ggatgtgccg gatgtgcccg
     3601 gtatgtcgtt taagattttc agcctgctgg cccagaagaa catcaacgtg gatatcattc
     3661 tgcagaccac cggccgcgac ggcaagaagg atatgagctt taccgtgccc ctgggcgacg
     3721 ccgaaaacgc tgtgtctgcg ctgaaaaacg ccaccagccg catcggcggc ggcgacatca
     3781 ccgtcgacaa gacctgcgcg aaggtctcca tcgtcggcgc gggtatgcag agccactccg
     3841 gcgtggcctc cacgatgttc gaggcgctgt tcaaccagaa tatcaacatc aagatgatct
     3901 ccaccagcga aatcaagatc agcgtcatca tcgatgaagc cgacgccgac aaggccgtcg
     3961 ccgccatcca cgacgcattc atcaactaag ttttttcagc aatatagagg gattgggtcg
     4021 gcccccatgc acagtgcggt gcatgggggc cgttggtgtt gtataaaggt ttacagcagc
     4081 tttttgccct gcgcaaactt tgcggtcagc gcacccctgc ccaggtaggc gtagcgctcc
     4141 aggcgctcgg tgggggtgaa ggtgcgcata tttttcagtg cggagtcgtc caaaaccagc
     4201 gcgtcaaagg cgtagccgtc ctcaaacagg ccgacgttct caccgaaatc ggcccagaag
     4261 tcaccgccgc ctacggtcgc catatacagc gcctcggtca tggtcagcgg tttctcgctc
     4321 tggtcgatgt accgccagcg cagcttgctg acctgcacgg ccgctgccat cacggcgaag
     4381 aggtccagcg tgtggccgcc cgccacgtcg ctgcccaggc cgatgcgcca gccgccgtgc
     4441 aggtacttgg cggcgggggc gatacccgca atcacgttct cgttgctggt ggggcagtgg
     4501 gcgatcatga cacggttgcg cttca
//
LOCUS       DBMDSQ010000155         4517 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_4558_length_4517_cov_1.640172, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000155 DBMDSQ010000000
VERSION     DBMDSQ010000155.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 4517)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4517)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4517
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4558_length_4517_cov_1.640172"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /locus_tag="ACZPAX_06480"
     CDS             <1..306
                     /locus_tag="ACZPAX_06480"
                     /inference="COORDINATES: protein motif:HMM:NF015431.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HIR1113580.1"
                     /translation="TEPFIESYPGMETDITLLFDKLKTKPHTSYSTMDITATYAMVSA
                     GLGISVNNAINHQHGYPGVVERPMDPPQLMEIGLACGENLAPAAQAFLEFVKTRMPE"
     gene            complement(517..1356)
                     /locus_tag="ACZPAX_06485"
     CDS             complement(517..1356)
                     /locus_tag="ACZPAX_06485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HIR1113581.1"
                     /translation="MAWMIVSDSSCEIRELERPAPGVQFALVPFKIRVGEREYVDLPT
                     LNTHQMLQAMTDYNGASTTACPSPEEWAEYFLQADNVFAFTISSNLSGSYNAAMAART
                     MVLEEHPEKKIFVLDTLSCSGALAGAAELANKLIGENQEFDDICFALKKFAESTHILF
                     ALASFDNLAKNGRVNKVVGFIAGRLNMRVLGRRTADGKIDFYFKTRGETRVLAKILEQ
                     MDEDGYDGVHPVLISECGNQTAAQLLHHGIDAKWPGAPVRIVPCSGLCSFYAQDQGII
                     ITY"
     gene            1554..2561
                     /locus_tag="ACZPAX_06490"
     CDS             1554..2561
                     /locus_tag="ACZPAX_06490"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012863.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HIR1113582.1"
                     /translation="MADFYTYRNEVASKRRKRKMFLVLIVILALLCAGAGFFWFKNSG
                     TPAPAASAEPVAATPETAPTATEAPTATPAAIAEPQRIVQAVDTAVWDKSDAVEQTID
                     TEYLNTDHRMVAVPMLGTVTKDYFNTVTFAGDSIASGLGIYSTGYNNAHYATYVSAGV
                     QTFVNNVSVKNAVTGTTETPMETIVSSQPDYLYILVGTNNLVVQGSEDSFLAYYERLI
                     DMLREQLDPGVKFYIQAIPGVQEDVVASKPGLDNTRIATVNDLLANMALRKGCYYINI
                     REVLTNPADGSQIDDYATKDGVHFNAAGYHAWADYLATHTVWNRRSVYSGENPYYIYG
                     A"
     gene            2821..>4517
                     /gene="glgB"
                     /locus_tag="ACZPAX_06495"
     CDS             2821..>4517
                     /gene="glgB"
                     /locus_tag="ACZPAX_06495"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HIR1113583.1"
                     /translation="MKQRKETPNSSDLPIYLFKQGNNQEAYRYFGAHLCEQNGEAGVV
                     FRVWAPHAKAVSIVGDFNSWTPGEHPMEKVTDGIWERFVPGIKQFDVYKYCITTPADE
                     LVYKADPYAFHTETRPSNGSKVYDIEGYTWGDAAWVKDEDKRDVINSPMSIYELQAGS
                     WKMKDPDNGVPYNYAELADELIPYIKDMGYTHVELLPITEYPFDGSWGYQVTGYFAPT
                     SRYGTPKDFMTFVDKLHQAGIGVILDWVPAHFPKDGYGLYMFDGAPCYEDPNPRRGEH
                     KEWGTMVFNYGMKEVESFLISSAMFWVDHYHIDGLRVDAVASMLYLDYNRKDGEWEQN
                     VKGGKENLEAIAFLQKLNTAILGRYPHKMMIAEESTAWPLVTKPASEGGLGFNFKWNM
                     GWMNDMLSYMKTDPLFRAGNHGKVTFSFFYAFSENFVLPISHDEVVHGKCSLLNKMPG
                     DYEEKFANLRTFFGYMMAHPGKKLLFMGQDFGQFIEWDEKKQLDWMLLSYDKHRELQG
                     YVKDLNHFYRDTPALWQVDYSWEGFQWIVPDDSKQSVIAFLRRDAAGKMILVVCNFNP
                     "
BASE COUNT         1051 a         1330 c         1238 g          898 t
ORIGIN      
        1 accgagccct tcatcgaaag ctaccccggc atggagacgg atatcacgct gctgttcgat
       61 aagctcaaga ccaagccgca cacgagctat tccaccatgg acatcacggc gacctacgcc
      121 atggtatcgg cggggctggg catcagcgtc aacaacgcca tcaaccacca gcacggctac
      181 cccggcgtgg tggagcgccc gatggacccg ccccagctga tggagattgg ccttgcctgc
      241 ggtgagaacc tcgcccctgc cgcccaggcg tttttagagt ttgtaaaaac acggatgccg
      301 gaataaaaaa gccttcccca ctcaggggaa agtgaccgaa ggtcggatga ggggcgcgtc
      361 tgccgtgcca tcccctaaag gggtgatgct ggcaaaaccg cccctcatca gtcagcggtc
      421 aagccgctga ctgcttctcc cgcaggagaa gccaaaccgc ccggaaagaa gctattgcac
      481 ttcttcccgg acggtttgtt ttatatttta ataaacttaa tacgttataa taatcccctg
      541 atcctgcgca taaaaactgc acagaccaga gcagggcacg atgcgcaccg gggcaccggg
      601 ccacttggcg tcaatgccgt ggtgtagcag ctgggcggcg gtctggttgc cgcactcgct
      661 gatcagcaca ggatgcacgc cgtcgtagcc gtcctcatcc atctgctcca gaatcttagc
      721 cagcacgcgg gtctcgccgc gcgtcttgaa gtagaagtca attttgccgt ccgctgtgcg
      781 ccggcccagc acgcgcatgt tcaggcggcc tgcgataaag cccaccacct tgtttacacg
      841 accgttcttg gccaggttgt caaagctcgc cagagcaaac aggatgtggg tgctctcggc
      901 aaactttttc agcgcaaagc agatatcatc aaattcctga ttctcgccga tcagcttgtt
      961 ggcaagctcg gcagcaccgg ccagcgcacc ggaacagctc agggtgtcca gcacgaaaat
     1021 cttcttttcg gggtgttctt ccaacaccat ggtgcgggcg gccatggcgg cgttgtagct
     1081 gccggacaga ttgctgctga tggtaaaggc aaacacgttg tccgcctgca aaaaatactc
     1141 ggcccattcc tcggggctgg ggcaggcggt ggtgctggca ccattgtagt cggtcatagc
     1201 ctgcagcatc tgatgggtgt tcagcgtggg caggtcaacg tactcgcgtt cgcctacacg
     1261 gattttaaac ggtacaaggg caaactgcac gcccggcgcg gggcgctcca actcgcggat
     1321 ctcgcaggaa ctatcggata cgatcatcca tgccataggg caggacctcc gttctgtgat
     1381 aattggtcac ggttattctt atctacatta tagaggcaca gaggaaaagc gtcaagttta
     1441 tttttgcggc agatcccgcc cggctctgta aaattttgcg ggcccacttg tacctgtgcg
     1501 gattgtggcg tataataacc atatatgttt ttgagataaa gatgggagct tctatggctg
     1561 atttctatac ctaccgcaat gaggtcgcca gcaaacggcg caagcggaaa atgtttctgg
     1621 tgctcatcgt aattttggcg ctgctctgcg ctggggctgg gttcttctgg ttcaagaaca
     1681 gcggcacgcc tgcgcccgct gcctcggcag agccggtggc cgccacgccg gagaccgctc
     1741 ccacggccac cgaggcaccg accgccacgc ccgccgccat cgccgagccg cagcgcatcg
     1801 tgcaggctgt ggataccgcc gtgtgggaca agtccgacgc cgttgaacag accatcgaca
     1861 ccgaatacct gaacaccgac catcgcatgg tggctgtgcc gatgctgggc actgtgacga
     1921 aggactactt caacaccgtg accttcgcgg gggacagcat tgcctcgggc ctgggcattt
     1981 acagcaccgg ctacaacaac gcccactatg cgacctacgt cagcgcgggc gtgcagacct
     2041 ttgtcaacaa cgtcagcgtc aagaacgccg tcaccggcac gacggaaacg ccgatggaga
     2101 ccatcgtctc cagccagccg gactacctct acatccttgt cggcaccaac aaccttgttg
     2161 tgcagggcag tgaggacagc ttccttgcct actatgagcg cctgatcgat atgctgcgag
     2221 aacagcttga ccccggcgtc aagttctaca tccaggccat ccccggcgtg caggaggatg
     2281 tcgtggcatc caagcccggc ctggacaaca cccgcattgc caccgtcaat gacctgctgg
     2341 ccaatatggc gctgcgcaag ggctgctact acatcaacat ccgcgaggtg ttgacaaacc
     2401 ccgccgacgg cagtcagatc gatgattatg ccaccaagga cggcgtgcac ttcaacgccg
     2461 caggctacca cgcctgggca gattacctgg cgacccacac ggtctggaac cgccgcagcg
     2521 tgtacagcgg cgagaacccc tactatattt acggcgcgta aaaaactttt ttaaattaca
     2581 caaaccccct gcaaggtgta aaaagtacct tgcagggggc tttttttgta caaaggtccg
     2641 aaaaacagcc cgttttatgt cgaacttgta caaaagcggc gggtaacata cgaaaatttt
     2701 agcagaatat tacacaaaag accttgaaat tccttttcaa aacaggtaat atagatacag
     2761 agattataca gagtcggtgt actgtatcgc gtacagcacc gctcaaaagg ggagattacg
     2821 ttgaaacagc gcaaagaaac gccaaatagc tccgatctgc ccatttatct gttcaaacag
     2881 ggcaacaatc aggaagctta ccgctacttt ggcgcccatc tgtgtgagca gaacggggag
     2941 gccggcgtgg tgttccgcgt ttgggcaccg cacgccaagg ccgtttccat cgtgggcgac
     3001 ttcaacagct ggacacccgg cgaacacccg atggaaaaag tcacggacgg catctgggaa
     3061 cggttcgtac ccggcatcaa acagtttgat gtctacaaat actgcatcac caccccggcg
     3121 gatgagctgg tctacaaggc cgacccctac gccttccaca ccgagacccg cccctccaac
     3181 ggcagcaagg tctatgatat cgagggctac acctggggcg acgccgcctg ggtcaaggac
     3241 gaggacaagc gggatgtcat caacagcccg atgtccattt atgaattgca ggccggcagc
     3301 tggaagatga aggatcccga caacggcgta ccctataatt acgccgagct ggccgacgaa
     3361 ctgatccctt acataaagga tatgggctac acccatgtgg agctgctgcc catcactgag
     3421 tatcccttcg acggcagctg gggctaccag gtcaccggtt actttgcccc caccagccgt
     3481 tacggcacgc ccaaagactt catgaccttc gtggataagc tccaccaggc aggcatcggc
     3541 gtcattctgg actgggtccc ggcccacttc ccgaaggatg gctacggcct gtatatgttc
     3601 gacggcgcac cctgctatga ggaccccaat ccccgccgtg gtgagcacaa agagtggggc
     3661 acgatggtct ttaactacgg catgaaggaa gtcgagagct tcctgatttc cagcgccatg
     3721 ttctgggtcg accactacca catcgacggt ctgcgtgtgg acgccgtagc cagtatgctc
     3781 tatctcgact acaaccgcaa ggatggcgag tgggagcaga acgtcaaggg cggcaaggaa
     3841 aacctggagg ccattgcctt cctgcaaaag ctgaacaccg ccattttggg tcgctacccc
     3901 cacaaaatga tgatcgccga ggaatccact gcctggccgc tggtcacaaa gcctgcgtcc
     3961 gaaggcggcc tgggcttcaa cttcaagtgg aacatgggct ggatgaacga tatgctcagc
     4021 tacatgaaga ctgacccgct cttccgtgcg ggcaaccacg gcaaggtcac cttcagcttc
     4081 ttctacgcgt tcagcgagaa ttttgtcctg cccatcagcc acgacgaggt cgtgcatgga
     4141 aagtgcagcc tgctgaacaa gatgcccggc gactacgaag aaaagtttgc gaacctgcgc
     4201 actttcttcg gctacatgat ggcccacccc ggtaagaagc tgctctttat gggccaggac
     4261 ttcggccagt tcatcgagtg ggacgagaaa aagcagctgg attggatgct gctgagctat
     4321 gacaagcacc gcgagctgca aggctacgtg aaagacttga accacttcta ccgtgatacg
     4381 cccgcattgt ggcaggtgga ttacagctgg gagggcttcc agtggatcgt gcccgatgac
     4441 agcaagcaga gtgtcatcgc cttcctgcgg cgcgacgcag cagggaagat gatcctggtc
     4501 gtctgcaact ttaaccc
//
LOCUS       DBMDSQ010000156         4511 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_4562_length_4511_cov_1.722449, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000156 DBMDSQ010000000
VERSION     DBMDSQ010000156.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 4511)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4511)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4511
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4562_length_4511_cov_1.722449"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..3444)
                     /locus_tag="ACZPAX_06500"
     CDS             complement(<1..3444)
                     /locus_tag="ACZPAX_06500"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005934311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HIR1113584.1"
                     /translation="MPETTPRQFTHLHVHTEYSLLDGACRIDRMFDQLKAMGQTACAI
                     TDHGVMYGCVAFFDAAKAAGIKPIIGCEVYVATRTRFDKVNRIDGNNHLILLCKNETG
                     YKNLIKMVSAGFTEGFYSKPRIDKELLEKHHDGLICLSACLAGEIPRAILAGDYDRAK
                     QTALWYRDLFGEGNYYIELQDHGLEEDQVVLPQLIRLARETGIPMAATNDAHYITKED
                     AKMQSILLCIQTGKTINDVDRMEFQTDEFYLKSTDEMYDLFSMVPEACENTNKIAEQC
                     NFAFTFGETKLPYFKAPDGMENQAYFEKLCWEGLERRYPGKVTDVLRERLTYEINVVK
                     TMGYTNYYLIVYDFINYAKSHDIPVGPGRGSGAGSLAAYCVGITDIDPIRYNLIFERF
                     LNPERVSMPDFDVDFCYERRQEVIDYVNEKYGRDHVAQIVTFGTMAARAAVRDVGRVM
                     GMSYQDVDRVAKLIPTDLKMTLKKALEVSPDLKTLYDGDSQVHELINTSLKVEGMPRH
                     ASTHAAGVVITRDPATEYVPLSTNDGLPVTQFNMVEIERLGLLKMDFLGLRTLTVIHD
                     TEMAVRHTKDPDFRIANLDYDDPDTYAMLAKGETEGIFQLESTGMTQVLVSMRPKNLE
                     DIIALISLYRPGPMDSIPTYLRNRKDPSQVTYKTPQMAHIVDVTNGVVIYQEQVMQIC
                     RELAGFSFGQADNVRRAMSKKKLKVMEAEREHFVHGCKEPGKECAGCVANGVPENVAN
                     EIYDDMISFASYAFNKSHAACYAYVAFQTAYLKCHYPHEFMAALLTSVLDNTSKVIEY
                     TTECQRLGIKVQQPDINISRGGFTADGGLIRFGLNAVKSVGRDVIDAVIKERTHQPYR
                     SLYDFCKRLRGGGLNRRALENLVKAGAFDNLGATRHGMLESVEGILKSVENDARQNLD
                     GQMDLFGLMGGGSDDKPADDYKIPNLPEYSAGELLKMEKEVSGLYLSGHPLDAYRAQI
                     AQIATCTIAQLLGEDAKQFDNQTVTFVCTIVKNKIMTTKSNTLMAFTTVEDLTGSMEM
                     IVFPRILAECRAALQENAVVVANGRVSVKEDEAARLLVEGVQPIDSYDPNKNFGMNRV
                     ERVKRETSGGGAAGYFLTVPARDCPEMHKVENLLCNIFDGGTVKVYFRFLDTGKAVLA
                     RH"
     gene            complement(3437..4369)
                     /gene="whiA"
                     /locus_tag="ACZPAX_06505"
     CDS             complement(3437..4369)
                     /gene="whiA"
                     /locus_tag="ACZPAX_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005934313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HIR1113585.1"
                     /translation="MSFSQEVKNEILRRKISKPCCNMAAAYAVACFGKYFDDRGVVLQ
                     TENEGVAHFAQKLYRRCGVQGTVISKERPTGPVYEFSVKDPAEVARMLALFGHTGKEP
                     TLRIRPENFRCQNCMQAFISTAFLCCGTMTDPEKSYNLEFLSTRHYLSQQLEAMLAEH
                     DFHPHRALRKGANTIYIKAADHIEDLLTFMGAGGAAMRIMDQRVYNEMRNKTNRLSNC
                     ETANLGKSVQAAVQVQLAIEKLQAAGAMNTLPEALRHTAKLRMQYPDLPLAKLAQKFD
                     PPVSKAGLSHRMKKIQEAAARLDTAPDEKETPHA"
     gene            complement(4386..4505)
                     /locus_tag="ACZPAX_06510"
     CDS             complement(4386..4505)
                     /locus_tag="ACZPAX_06510"
                     /inference="COORDINATES: protein motif:HMM:NF046811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RapZ C-terminal domain-containing protein"
                     /protein_id="HIR1113586.1"
                     /translation="MGCTGGKHRSITFARKIGEFCEKLGYAPSVQHRDVNRSL"
BASE COUNT          872 a         1260 c         1333 g         1046 t
ORIGIN      
        1 gtgccgggcc agcaccgctt tcccggtgtc caggaagcgg aagtatacct tgactgtgcc
       61 gccgtcaaag atgttgcaca aaagattttc aaccttgtgc atttccggac agtcccgagc
      121 aggcacggtc aggaaatacc cggcggcccc tcccccgctg gtctcgcgtt tgacacgttc
      181 gacacggttc atgccaaagt ttttgttcgg gtcataactg tcaatgggct gcacgccctc
      241 gaccagcaac cgggcggcct cgtcctcctt gacggagacg cggccattgg cgacgacgac
      301 ggcgttttcc tgcaaggctg cgcggcactc tgccaggatg cgcgggaaaa cgatcatctc
      361 catgctgccg gtcagatcct cgacggtggt aaaggccatc agggtgttgg actttgtcgt
      421 catgattttg ttcttgacga tggtacagac aaaggtgaca gtctggttgt caaactgctt
      481 ggcgtcctcc cccagcagct gggcgatggt gcaggtagct atctgcgcga tctgggcgcg
      541 gtaggcgtcc agcggatgac cggacaggta gaggccggac acctcttttt ccattttcag
      601 cagttcgcca gccgaatatt ccggcaggtt cgggatttta taatcgtcgg caggcttgtc
      661 gtcgctgccg ccgcccatca ggccaaaaag atccatctga ccgtccaggt tctggcgggc
      721 atcgttctcg acgctcttca agatgccctc gacgctttcc agcataccgt ggcgggtagc
      781 accgaggttg tcgaatgcac cggccttgac gaggttttcc agcgcgcggc ggttgaggcc
      841 gccgccccgc aagcgcttac agaaatcgta cagactgcgg taaggctggt gggtgcgttc
      901 cttaatgacg gcgtcaatta cgtcgcgccc cacgctcttg acggcgttca ggccgaagcg
      961 gataaggccg ccatccgccg taaagccgcc gcgggagata ttgatatccg gctgctggac
     1021 cttgatgccc agacgctggc actcggtcgt atattcaata acttttgacg tattatcgag
     1081 cacactggtc aggagtgccg ccataaattc atgcggatag tggcacttta aatacgccgt
     1141 ctggaatgcc acgtaggcat agcaagcggc atgggatttg ttgaacgcat acgacgcaaa
     1201 gctgatcata tcgtcgtaaa tctcgttggc aacgttctcg ggcacgccgt tggccacgca
     1261 gcccgcacac tcctttcccg gttccttgca gccgtggaca aagtgctcgc gttcagcctc
     1321 catgaccttg agctttttct tactcattgc gcggcggaca ttgtcagcct gaccgaagct
     1381 gaacccggcc aactcgcggc agatctgcat aacctgttcc tggtagatga caacgccgtt
     1441 ggtaacgtca acgatatgcg ccatctgcgg cgttttgtag gtcacttggg aagggtcctt
     1501 gcggttgcgc aggtaggtcg gaatggaatc catcgggccg gggcggtaga gcgaaatcaa
     1561 ggcaatgata tcttccaggt tctttggccg catgctgaca agcacctggg tcatgccggt
     1621 agattcaagc tggaagatgc cctccgtctc accttttgcc agcatggcgt aggtgtcggg
     1681 gtcgtcgtaa tcgaggtttg caatacggaa atccggatct ttggtatggc gcacggccat
     1741 ctcggtatca tggatgacgg tcagcgtgcg cagacccaga aagtccattt tcaaaaggcc
     1801 cagccgctcg atttccacca tgttgaattg ggtgaccggc aagccgtcgt tcgtggacag
     1861 cggtacatac tcggtggccg ggtcgcgggt aatgacgacg cccgccgcgt gggtggatgc
     1921 atggcgcggc atcccctcga ctttcagcga ggtgttgatg agttcatgca cctggctgtc
     1981 gccgtcgtag agtgttttca aatcggggct gacttccagc gcctttttca gcgtcatttt
     2041 caggtcggtg ggaatgagct ttgccacccg gtccacatcc tggtagctca tgcccataac
     2101 gcggcccaca tcgcgcacgg cagcgcgggc ggccatcgta ccaaacgtca cgatctgggc
     2161 cacatggtca cggccgtatt tctcgttgac atagtcaatg acctcctggc ggcgctcgta
     2221 gcagaagtcg acgtcgaagt cgggcattga gacgcgctct gggttcaaaa aacgctcgaa
     2281 gatcaggttg tagcggatcg ggtcgatatc ggtgatgccg acacagtagg ccgccagact
     2341 gcccgcgccg gagccacgtc ccggaccgac cgggatatcg tggcttttgg cgtagttgat
     2401 aaagtcgtag acaatgaggt agtagttcgt gtagcccatc gtcttgacga cgttgatctc
     2461 ataggtcagg cgctcacgca ggacatcggt cactttaccg gggtaacggc gctccaggcc
     2521 ctcccagcag agcttttcaa aatatgcctg gttctccatg ccgtcgggcg ctttgaagta
     2581 cggcagcttc gtctcgccga aggtaaacgc gaagttgcac tgctcggcaa ttttgttcgt
     2641 gttctcgcag gcttcgggca ccatggagaa gaggtcgtac atctcatcgg tggatttcag
     2701 atagaactcg tcggtctgga actccatccg atccacatcg ttgatggtct tgccggtctg
     2761 gatgcaaagc agaatgctct gcatcttggc gtcctctttg gtgatataat gggcgtcgtt
     2821 cgttgccgcc atcgggatgc cggtctcccg cgccaagcgg atgagctgtg gcagcacgac
     2881 ctggtcttct tcaaggccgt ggtcctgcaa ttcgatgtaa tagttgccct cgccgaacaa
     2941 gtcccgatac cacagcgctg tctgcttggc gcggtcatag tcccctgcca gaatcgcgcg
     3001 gggtatctcg cccgccaagc aggcggacag gcaaatcaga ccatcgtggt gcttttccag
     3061 cagttcctta tcgatacgcg gcttggagta aaaaccctcg gtaaagccgg cagacaccat
     3121 cttgataagg ttcttgtagc ctgtctcgtt tttgcagagc agaatcaggt ggttgttgcc
     3181 gtcgatacgg ttaaccttat caaagcgggt gcgggtggct acatagacct cacagccgat
     3241 gatgggcttg atgcccgccg ctttggccgc atcaaagaaa gccacgcagc cgtacatgac
     3301 accgtggtcg gtgatagcgc acgccgtctg ccccatcgct ttcagctggt caaacatccg
     3361 gtcaatgcgg caggcaccgt ccaacaagct gtactcggtg tggacatgaa ggtgtgtgaa
     3421 ttgtctcggg gtcgtttcag gcatggggtg tttccttttc gtcaggggcc gtgtccaggc
     3481 gggcggcagc ctcctgtatc ttcttcatcc ggtgggaaag cccggctttg ctcaccggcg
     3541 gatcaaactt ctgggccagc tttgccagcg gcaggtcggg gtactgcatc cgcagcttgg
     3601 cggtgtggcg cagggcctcg ggcagggtgt tcatagctcc cgccgcctgc agcttttcaa
     3661 tggcaagctg cacctgtacg gcggcctgga cgcttttgcc caggttggct gtctcgcagt
     3721 tggaaagacg gttcgtcttg ttgcgcatct cgttgtaaac acgctgatcc ataatgcgca
     3781 tggccgcgcc gcccgccccc ataaaggtca gcagatcttc gatatggtcg gcggccttga
     3841 tataaatggt gttggccccc ttgcgcaggg cgcggtgggg atgaaaatca tgctcggcca
     3901 gcatggcttc cagctgttgg gaaaggtaat gccgggttga gaggaattcc agattgtagc
     3961 ttttttcagg gtcggtcatc gtgccgcagc acaaaaacgc cgtggagata aatgcctgca
     4021 tacagttctg gcatctgaaa ttttccggac ggatgcgcag ggtaggctcc ttgccagtgt
     4081 ggccaaacag tgccagcatc ctggcaacct cggctgggtc ctttacgctg aactcataca
     4141 ccgggcctgt ggggcgttcc ttgctgataa cagtgccctg cacaccgcag cgacggtaga
     4201 gcttttgggc aaaatgggca acgccctcgt tttcggtctg taagacaaca ccgcggtcat
     4261 caaagtattt gccaaaacag gccaccgcat acgcggcggc catgttgcag cagggtttgc
     4321 tgattttgcg gcgcagtatt tcgtttttca cttcttgaga aaagctcaag gtggtggtct
     4381 ccttattata aacttcggtt cacgtcgcgg tgctgcacgc tgggggcata gccgagtttc
     4441 tcgcagaact cgccaatctt ccgtgcgaac gtgatggagc ggtgcttgcc gccggtgcag
     4501 cccaccgcga t
//
LOCUS       DBMDSQ010000157         4504 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_4567_length_4504_cov_2.372019, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000157 DBMDSQ010000000
VERSION     DBMDSQ010000157.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 4504)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4504)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4504
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4567_length_4504_cov_2.372019"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..753
                     /locus_tag="ACZPAX_06515"
     CDS             <1..753
                     /locus_tag="ACZPAX_06515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HIR1113587.1"
                     /translation="QQQILTLLKENHIDVIVPLIDQDLFTWAADAPELAALGVRSTAP
                     LRQTAQLLTDKKAMAAFLQAHGLPTPPLVAPEQMEPGRAYIEKREVGCGSVGLRTVIG
                     GEDYTPHPGSILQEKCDGGDTEITAEVFNAQGKLRVFCRERVVTKSGVCTKMRPLHIP
                     EIEDYICTLTGLLPCPATFCAQFMLHRGRWNLIDCNLRIGAGTAMASAAGFQLVRAFW
                     ANLCGQPVPDEWLQADPTVKTVLRVYQEVVVR"
     gene            750..1376
                     /locus_tag="ACZPAX_06520"
     CDS             750..1376
                     /locus_tag="ACZPAX_06520"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR1113588.1"
                     /translation="MTLYCDVDGTLLDSSARHEKLLRDLLAVRDLAWPAAAPDYMRYK
                     ADGHSTKAWLALAGFAPEVCNEIAAAWREGIEKPEYLAMDRPYPDTLDFLRWLRASGR
                     RCALISARQNADALRETLDRCGILPLVEELLVVLPVQAEQRKAALLRPRIAPGDAMLG
                     DTEVDKTCAETLGLPCAVLDRGFRSAAFWEAHRVKALHGLEEAKRWLR"
     gene            1555..2616
                     /locus_tag="ACZPAX_06525"
     CDS             1555..2616
                     /locus_tag="ACZPAX_06525"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HIR1113589.1"
                     /translation="MNQPKVSVLISFYNLAPYVDKTMETVLAQKTNFPVEVLCADDGS
                     DDGTIEKLRVWEQKYPDTVRVFVMDRIPGAKYEANDRIKRMNAIRGRLFYEARGEYVS
                     YLDGDDFYTDDHKLQKQADVLDADTEHKYIGCGHNGCYYWESTGKTVPIERPIRECSL
                     TAEEYWSFLYVHTNALLLRNLGLQGIDPYKSGIPIIDNPLTFQFLPYGDIYYLPDCMF
                     NYRQIEGSTYHRRSPYQNDILTALILYEQRRITRRFDAAGLVRFLREYEDLFKNRRDP
                     QLTKDAQTYMENIRTYRADRLRRIVNYNNESAVSRLWCDIENPVWFFITKIHRHFIWK
                     PKVRALLEEARVKTEKGEC"
     gene            2620..3723
                     /locus_tag="ACZPAX_06530"
     CDS             2620..3723
                     /locus_tag="ACZPAX_06530"
                     /inference="COORDINATES: protein motif:HMM:NF025663.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp fold amidoligase family protein"
                     /protein_id="HIR1113590.1"
                     /translation="MEDMSELTAEEQAKLLRHELEAVYGSSSYKIGRAVTWLPRNAKR
                     GLKYLLHNGPVVSAKYIYTYAKYHKIANENYAYWACLQKKDYPEALKKWFLETNYTHT
                     PLNLEHPKTFSEKTQWLKLNGGFEDVYPLVDKYAVREWVKEKIGEEYLIPLLGVWDNF
                     DDIDFDALPDKFMLKVNHGAGWNIAVQDKSKFDKADAKRKIEGWLKLNYCYLMGGLDV
                     QYIHIKPRIIAEKFIENDGGDLYDYKIFCFDGEPKIILHIEERYTDKEERMFFYDTQW
                     NQLPFNINVPLEMVDIPKPENLDKMLDIARTLSQGYPCVRVDLYSLNDGSIKFGEMTF
                     TTESGISRWHPESANEYMGSLIHLPGVDDKGDN"
     gene            3723..>4504
                     /locus_tag="ACZPAX_06535"
     CDS             3723..>4504
                     /locus_tag="ACZPAX_06535"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR1113591.1"
                     /translation="MPTVSILVPVYKAEKTLDACVESILAQSYADFELLLIDDASPDH
                     CPEMCDAWAEKDPRIRTLHPAKTGPGPSGARNAGLDAAKGQWLTMVDSDDTVAPDLLE
                     KLLAGAERSGAELVLCNCRPVTEDGAEHPLPADEQFAKETVLDVNAFWDAFNTPWINQ
                     FTGTAHRLYAAKLFDGVRYPLGMLHEDYYVLPDLIARCTKILCLPFTGYYVLRHAGSI
                     TDGAKHEVRLAMTKGDIHRAEYFLQNGWYDRAEGALTDAALF"
BASE COUNT          992 a         1401 c         1348 g          763 t
ORIGIN      
        1 caacaacaga tcctcactct gttgaaagaa aaccacatcg acgtcattgt gccgctcatc
       61 gaccaggatt tgttcacctg ggcggcggat gcgccagagc tggccgcatt gggcgtgcgc
      121 tccaccgcgc cgctgcggca gacggcccag ctgctcaccg ataaaaaggc catggccgca
      181 tttttgcagg cccacggcct gcccaccccg ccgctcgtcg cgcctgaaca aatggagccg
      241 ggccgcgcct acatcgaaaa gcgcgaggtc ggctgcgggt ccgtcgggct gcgcacggtc
      301 atcggcggcg aggactacac cccgcacccc ggcagcattt tacaggaaaa atgcgacggc
      361 ggcgacaccg aaatcaccgc cgaagttttc aacgcccagg gcaagctgcg ggtgttctgc
      421 cgtgagcgcg tcgtgacaaa atccggcgtc tgtacgaaga tgcggcccct gcacatcccc
      481 gaaatcgagg attacatctg cacactgacc ggactgctgc cctgcccggc gacgttttgc
      541 gcccagttca tgctgcaccg cgggcggtgg aacctcatcg actgcaacct gcgcatcggc
      601 gcgggcacgg cgatggcatc cgccgccggg ttccagctgg tgcgggcgtt ctgggcgaac
      661 ctctgcggcc agcctgtgcc cgacgaatgg ctgcaggccg accccacggt caagactgtg
      721 ctgcgggtct accaggaagt ggtggtacgg tgacgctcta ctgtgatgtg gacggcacgc
      781 tgctggacag cagcgcccgc catgaaaagc tgttgcggga cctgctggcg gtccgggacc
      841 ttgcctggcc cgccgccgcg cccgactata tgcgctataa ggctgacggc cacagcacca
      901 aggcgtggct ggccctggcc gggtttgcgc cggaagtctg caatgaaatt gccgccgcct
      961 ggcgcgaggg cattgaaaaa ccggagtatc tggccatgga caggccttac ccagatacgt
     1021 tggatttcct gcgttggctg cgggcaagcg ggcggcgctg tgcgttgatc tcggcccggc
     1081 agaatgccga cgccctgcgg gaaacgcttg accgatgcgg catcctgccg ctggtggagg
     1141 aactgctggt cgtgctgccg gtgcaggccg agcagcgcaa agccgccctg ctgcgcccgc
     1201 gcatcgcccc gggggacgcc atgctgggtg acaccgaggt ggacaagacc tgcgccgaga
     1261 cgctgggcct gccctgtgcc gtgctggacc ggggcttccg cagtgcggcg ttttgggaag
     1321 cgcacagggt aaaggcattg catggtttgg aagaggccaa gcggtggctg cggtaatgtg
     1381 ccgcgggcgg atatggaatc cgcccctacg gaccggcagg cagcgttgta ggggccgggc
     1441 atgcccggcc cgcagccgaa gggcagccgc acggcaaaga caaggcacgt gggtcgtcga
     1501 ggacgccgac ccctacaaaa cgaccattat aacttttaaa gaaggacaaa cactatgaac
     1561 cagcccaagg tcagcgtgct gatctcgttt tacaatctgg ccccctatgt ggacaagacg
     1621 atggagacgg tgcttgcgca gaaaacgaac tttcccgtcg aggtcctctg cgcggacgat
     1681 ggctccgacg acggcaccat tgaaaagctg cgcgtgtggg agcagaaata cccagacacc
     1741 gtccgcgtgt tcgtcatgga ccgcatcccc ggcgcaaaat atgaggccaa cgaccgcatc
     1801 aagcggatga acgccatccg cgggcggctg ttctatgagg ccaggggcga gtatgtcagc
     1861 tacctggacg gcgacgactt ctacaccgac gaccacaagc tgcaaaagca ggccgatgtg
     1921 ctggacgccg acacagagca taaatacatc ggctgcggcc acaacggctg ctattactgg
     1981 gagagcaccg gcaagaccgt ccccatcgag cggcccatcc gggagtgcag cctgactgcc
     2041 gaggaatatt ggagctttct ctacgtccac accaacgcgc tgctgctgcg caacttgggg
     2101 ctgcaaggca tcgaccccta caagagcggc atccccatca tcgataaccc gctgaccttc
     2161 cagttcttgc cctacggtga tatctactat ctgcccgact gtatgttcaa ctaccgccag
     2221 atcgagggca gcacctacca ccgccgcagc ccctaccaga acgatatcct gacggcgctg
     2281 attctgtacg agcagcgccg catcacccgc agatttgacg cggcgggcct ggtgcgtttc
     2341 ctgcgcgagt atgaggatct ttttaaaaac cgcagggacc cgcagctgac aaaggacgcg
     2401 cagacctata tggagaacat tcgcacctac cgcgctgacc gcctgcgccg catcgtcaac
     2461 tataacaacg agagcgcggt atcccgcctg tggtgcgata tcgagaaccc ggtgtggttc
     2521 tttattacaa aaatccaccg ccacttcatc tggaaaccca aggtgcgggc gctgctggaa
     2581 gaagcccgcg tgaaaaccga aaaaggggag tgctgaacca tggaagatat gagcgaactg
     2641 accgccgaag aacaggccaa actgctgcgc catgagttgg aggccgtcta cggctcgtcg
     2701 tcgtacaaaa tcggccgcgc tgtgacctgg ctgccccgca acgccaagcg cgggctgaag
     2761 taccttttgc acaacggccc ggtcgtcagc gcaaaatata tctataccta cgcgaaatat
     2821 cataagatcg ccaacgaaaa ctacgcctac tgggcctgcc tgcaaaagaa ggactacccc
     2881 gaggcgctga aaaagtggtt cctggaaacg aactacaccc acacgccgct gaacctcgag
     2941 caccccaaga ccttcagcga aaagacccag tggctcaagc tcaacggcgg gtttgaggac
     3001 gtctaccccc tcgttgacaa atacgccgtg cgcgaatggg tcaaggagaa gatcggcgag
     3061 gaatacctga ttccgctgct gggcgtctgg gataactttg acgatatcga ctttgacgcc
     3121 ctgccggaca aattcatgct caaggtcaac cacggcgcgg gttggaatat cgccgtgcag
     3181 gataagtcaa aattcgacaa ggccgacgcc aagaggaaga tcgagggctg gctcaagctg
     3241 aactactgct acctgatggg cggcctggat gtgcagtaca tccacatcaa accgcgcatc
     3301 atcgctgaaa aattcataga gaacgacggc ggcgacctat acgactacaa aatcttctgc
     3361 ttcgacggtg agccgaaaat catcctgcac atcgaggagc gctacaccga caaggaagag
     3421 cggatgttct tctacgacac ccagtggaac cagctgccct tcaacatcaa tgtcccgctg
     3481 gaaatggtcg atatccccaa gccggaaaac ctcgataaga tgctggatat tgcccgcacc
     3541 ctgagccagg gctacccctg cgtgcgcgtt gatttgtact cgctgaacga tggcagcatc
     3601 aaattcggcg agatgacctt caccaccgag agcggcattt cccgctggca ccccgagagc
     3661 gccaacgagt acatgggcag cctgatccat ctgcccggcg tcgacgacaa aggagacaac
     3721 taatgcccac cgtcagtatc ctggtgccgg tctacaaggc cgaaaaaacg ctggacgcct
     3781 gtgtggaaag catcctggcg cagagctatg cggattttga gctgctgctc atcgacgatg
     3841 ccagccccga ccattgcccg gaaatgtgcg atgcctgggc ggaaaaagac ccccgcatcc
     3901 gcaccctgca cccggctaag accgggccgg ggccgtccgg tgcgcgcaac gccgggctgg
     3961 atgccgccaa gggccaatgg ctcacgatgg tggacagcga cgacaccgtt gcccctgacc
     4021 tgctggaaaa gctgctggcg ggtgcggaaa ggagcggcgc ggagctggtg ctctgcaact
     4081 gccgcccggt gacagaggac ggcgcagagc acccattgcc cgcagacgaa cagttcgcaa
     4141 aagagaccgt tctggacgtg aacgctttct gggatgcctt caacacgccc tggatcaacc
     4201 agttcaccgg cacggcccac cggctgtacg ccgccaagct gtttgacggc gtgcgctatc
     4261 cgctggggat gctgcacgag gattactacg tgctgccgga cctgatcgcc cgctgcacaa
     4321 aaattttgtg cctgcccttt accggctact acgtgctgcg ccacgcaggc agcatcaccg
     4381 acggcgccaa acatgaggtg cggctggcca tgacgaaggg ggatatccac cgggcggagt
     4441 atttcctgca aaacggctgg tacgaccgcg ccgagggtgc cctgaccgac gccgcgctgt
     4501 tctt
//
LOCUS       DBMDSQ010000158         4491 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_4586_length_4491_cov_1.862187, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000158 DBMDSQ010000000
VERSION     DBMDSQ010000158.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 4491)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4491)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4491
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4586_length_4491_cov_1.862187"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1266)
                     /locus_tag="ACZPAX_06540"
     CDS             complement(<1..1266)
                     /locus_tag="ACZPAX_06540"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HIR1113592.1"
                     /translation="MPKLLCPHCLQPLDDGYHGDCPHCGRTLENRNPEGALPFGTQLG
                     GKYTVGAYLSADGDGLAYRGVLNDEKRFVLIKEYFPVTLCNGRSAAGALMPKEEKEVL
                     FKTSRMDFKDLYDDLKNLTPATGLSTILDVMEENNTVYAVEESEKGMTLSHYLHLRSR
                     TLTPAEARTLLQPIMEGVAQLHRSGLIHRGICPDNILLPIDGTARLTGYGTLALRTGG
                     SELKSQLYPGYAAPEQYSAAEFSGRYTDVYALAAVCYKMVTGQTPVAAPQRRVRDSLE
                     SAHSLEAGVPTWFSQVLACAMRLDPAKRMQTVPELMSALTDPNVANAMFERGDNQIST
                     RKIMGVSLVVIFVLVVLLLWSLLGGSKDGTTPTSTPQTVTSTVAGESSTEKATVPNLV
                     GLRYATDIKGNDAYDGYRIVMVEQSSDTVA"
     gene            complement(1388..2125)
                     /locus_tag="ACZPAX_06545"
     CDS             complement(1388..2125)
                     /locus_tag="ACZPAX_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR1113593.1"
                     /translation="MSGHSKWNNIKRKKEKTDGARAKVFTKIGREIAVAVKEGGGNPA
                     SNSKLAALIAKAKANSVPNDNIQRIIKRAEGGDKTEYEAITYEGYGPGGIAVMVETLT
                     DNRNRTAANMRHYFDKFGGNLGQMGCVGFMFTQKGVIVVDLEDKDPDELMMDALDAGA
                     DDFDAGEDAAEVTTSPENFTAVCDALEKKGYKFISADVAQVPSTTTTLTDPDQLVQMG
                     KLLDALDDDDDVQNAWHTLENEEDLDR"
     gene            complement(2237..3610)
                     /locus_tag="ACZPAX_06550"
     CDS             complement(2237..3610)
                     /locus_tag="ACZPAX_06550"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015563952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HIR1113594.1"
                     /translation="MEFPQDFTARERALLGDRFDELYAYATPQPARGVTVNALRCTPD
                     WFAKHMDFVAEPSKFCPAAFTTAPDFRPGRHPWHHAGVLYAQEPSASAPAALLDVQPG
                     MRVADLCAAPGGKTSQLAAALQGRGLLLANEFVAARAEILRQNLERMGVANAIITNED
                     TARLAAAMPGRFDRVLVDAPCSGEGMFRKEAAATAQHNDGLVAHCAELGAEILENAAA
                     LLAPGGVLVYSTCTFAPDEDEGQIANFLAKHPEFTLCDLSACGFGRPGEGNRAPDHPD
                     FHAEYTRRIWPADGGEGHFMAKLQKAAAAEAPAPPKVKPPKALKAPAEWLDFAKTYFP
                     ALASRPLAGAGEWLLLPAPGSEGLNTAKLRVVRGGVLVGSVLKKRFQPAHALFMAYGT
                     QCTNREELTLADPRTAAWLRGEEIDAATAQNGWCAVLVDGFPLGGGKVSGGRIKNHYP
                     KGLRNLQ"
     gene            3714..3851
                     /locus_tag="ACZPAX_06555"
     CDS             3714..3851
                     /locus_tag="ACZPAX_06555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113595.1"
                     /translation="MAKNQTNNSTNRTSDSTQKTTNRTQQKTTNRSTNTTNTTNSKNC
                     K"
     gene            complement(3958..4362)
                     /locus_tag="ACZPAX_06560"
     CDS             complement(3958..4362)
                     /locus_tag="ACZPAX_06560"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HIR1113596.1"
                     /translation="MLFTQKSEVDIHEMSPLALAFVGDAVLELLVRARLVGTTRLQPN
                     RLHTVATHYVSAHAQNLELDIIEPMLTDAEAAVLRRGKNASKATVAKHATVQEYRAST
                     GFECLLGWLYLQGKNDRIEELFNALWEGYKPE"
     gene            complement(4375..>4491)
                     /locus_tag="ACZPAX_06565"
     CDS             complement(4375..>4491)
                     /locus_tag="ACZPAX_06565"
                     /inference="COORDINATES: protein motif:HMM:NF020830.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1967 domain-containing protein"
                     /protein_id="HIR1113597.1"
                     /translation="QYFQTQLGDSGILDELVAQDVEEDDTIKIGEYEFDYLY"
BASE COUNT          802 a         1395 c         1392 g          902 t
ORIGIN      
        1 ggccactgtg tccgagcttt gttccaccat gacgatgcgg tagccgtcgt aggcatcgtt
       61 gcccttgata tcggtcgcgt accgcaggcc aacgaggttc ggcaccgtcg ctttttcggt
      121 gctgctctct cctgctaccg tggacgtcac cgtctgcggg gtggatgttg gcgtcgtgcc
      181 gtccttgctg ccgcccagca ggctccagag cagcagtacc accaacacaa agataaccac
      241 cagcgacaca cccataattt tgcgggtgct gatctggttg tcaccgcgct caaacattgc
      301 gttggcaacg ttcgggtcag tcagggcgct catcaattcg ggcacggtct gcatacgctt
      361 ggccggatcc agccgcatgg cgcaggccag cacctgggaa aaccaggtcg gcacgcccgc
      421 ttccaggctg tgggcggatt ccaggctgtc ccgcacgcgg cgctggggcg cggccaccgg
      481 cgtttgccct gtgaccatct tgtagcagac cgccgccagg gcgtacacgt ctgtgtagcg
      541 gccgctgaac tcggcggcgg agtactgttc cggcgctgcg tagccggggt aaagctggct
      601 tttcagctcg ctgccgccgg tgcgcagcgc cagcgtgccg tagccggtca gccgggccgt
      661 accgtcgatg ggcagcagga tattgtccgg gcagatgccg cggtggatca ggcccgagcg
      721 gtgcagctgg gccacgccct ccatgatggg ctgtaaaagc gtccgcgcct cggcgggggt
      781 cagcgtgcgg ctgcgcaggt gcaggtagtg ggacagggtc atgcccttct cgctttcctc
      841 cacagcgtag accgtgttgt tttcctccat gacgtccaaa atcgtggaca ggccggtggc
      901 aggggtcagg tttttcagat catcgtacag atccttgaag tccatgcggc tggtcttgaa
      961 caggacctct ttttcctctt tgggcatcag ggcaccggcg gcgctgcggc cattgcagag
     1021 cgtgaccggg aaatattcct tgatgaggac aaaacgtttt tcgtcgttca gcacgccgcg
     1081 gtaggccagg ccgtcaccgt cggcgctcag gtacgcgccc acggtgtact tgccgcccag
     1141 ctgggtgcca aagggcagtg cgccctcggg gttgcggttt tctaatgtgc ggccgcagtg
     1201 ggggcagtcg ccgtgatagc cgtcatccag cggctgcagg cagtgcgggc aaaggagttt
     1261 gggcatagta acgctccttt tcaattaaaa acatgcttgt aggggccggg catgcccggc
     1321 cctgaacttt gccgtacaaa aaaagagagc aaaagttgcc cttcactctc tttttgcatt
     1381 ctgtggttta gcggtcgagg tcttcctcgt tctccagggt gtgccaggcg ttctggacat
     1441 cgtcatcgtc gtccagggca tccagcagct tgcccatctg gaccagctgg tcggggtcgg
     1501 tcagcgtggt ggtggtggaa ggcacctgtg ccacgtcggc ggagatgaac ttgtagccct
     1561 tcttctccag cgcgtcgcag acagcggtga agttctcggg gctggtggtg acctcggcgg
     1621 catcctcgcc tgcatcgaaa tcgtcagcac cagcgtccag cgcgtccatc atcagctcat
     1681 cggggtcctt gtcctccagg tcaacgacga tgacgccctt ctgggtgaac atgaagccga
     1741 cacagcccat ctggcccagg ttgccgccga acttatcaaa gtagtggcgc atgttggcag
     1801 ccgtgcggtt gcggttatcg gtcagggtct cgaccatgac ggcgatgccg ccggggccgt
     1861 agccctcgta ggtgattgct tcgtactcgg tcttgtcgcc gccctcggcg cgcttgatga
     1921 tgcgctggat gttgtcgttg ggcacgctgt tggccttggc cttggcgatc agggcagcca
     1981 gcttggagtt ggaagcaggg ttgccgccgc cctccttaac ggcaacggcg atctcacggc
     2041 cgatcttggt gaaaaccttg gcgcgggcgc cgtcggtctt ttccttttta cgtttgatgt
     2101 tattccattt gctatgtccg gacatggaaa aatcccccta atttgtattc acacaggcaa
     2161 cggcgttgca gccgataatt accagtaaat tattatagca caacaggtcg tacttttcaa
     2221 gaggggccgc agcggattat tgcagattcc gcagaccctt tggataatga tttttgatgc
     2281 ggccgccgct gaccttgccg ccgcccagcg gaaaaccgtc caccagcacg gcgcaccagc
     2341 cgttttgcgc ggtggcggcg tcgatctcct cgccccgcag ccaggcggcg gtgcgcggat
     2401 cggccagggt cagctcctcg cggttcgtac actgggtacc ataggccatg aacagcgcgt
     2461 gggcgggctg gaaccgcttt ttcagcacgc tgcccaccag cacgccgccg cgcaccacgc
     2521 gcagcttggc ggtgttcagc ccctcgctgc ccggtgcggg cagcagcagc cactcccccg
     2581 cccctgccag cgggcgggat gccagcgccg ggaagtaggt cttggcaaag tcgagccatt
     2641 ccgccggagc tttcagcgcc ttgggcggct tgactttcgg cggggcgggc gcttcagcgg
     2701 cggcggcttt ttgcagcttc gccataaaat gcccctcgcc gccgtcggca ggccagatgc
     2761 ggcgggtata ttcggcgtgg aagtccgggt ggtccggggc gcggttgccc tcgcccgggc
     2821 ggccaaaacc gcaggccgac aggtcacaga gggtgaactc ggggtgcttt gcaagaaagt
     2881 ttgcaatctg cccttcatcc tcatccgggg caaacgtgca ggtcgaatag accaatacac
     2941 cgcccggggc cagcagggcc gccgcatttt cgagaatttc cgcgcccaac tcggcgcagt
     3001 gggccacaag gccgtcattg tgctgagctg tggcggcggc ctccttgcgg aacatcccct
     3061 cgcccgagca gggcgcatcg acgagcaccc ggtcaaaccg gccgggcatg gcggcggcca
     3121 ggcgggccgt gtcctcgttg gtaatgatgg cgttcgccac acccatccgc tccaaattct
     3181 ggcgcagaat ttccgcgcgg gccgcgacaa attcatttgc cagcagcagg ccccggccct
     3241 gcaaagccgc tgccagctgg cttgtcttgc cgccgggcgc ggcgcagagg tcggccaccc
     3301 gcataccggg ctgcacatcc agcagcgctg ccggggcgga cgcgctgggt tcctgggcgt
     3361 agagcacacc tgcatggtgc caggggtggc ggcccgggcg gaagtctggt gccgtcgtaa
     3421 aggcggcggg acagaatttc gacggttctg caacaaaatc catatgtttg gcaaaccaat
     3481 ccggcgtgca gcgcagcgcg ttgaccgtga cacctcgggc gggctgcggc gtggcgtagg
     3541 catacagctc gtcaaagcgg tcgcccaaga gcgcacgctc ccgcgcggtg aaatcctgtg
     3601 gaaattccat gaccgttccc cctgcataaa aaagccatgg gcgggcaaac tacccttgca
     3661 gtccaaaaac gactgtatat cgttttctta cctgcgaaag gagaatacca cctatggcaa
     3721 agaatcagac taacaattcg accaaccgca cgagcgacag cacccagaaa acgacgaacc
     3781 gcacccagca gaaaaccact aaccgcagca ccaacaccac caacaccacc aacagcaaga
     3841 actgcaagtg atgcagggcg gcgttcctgc tgcaccccat taccgggcaa ttgcgggaaa
     3901 cgcgcccctc atccggcctt cggccacctt ccccctaggg gggaaggctt ttttgtttta
     3961 ctccggctta tacccttccc acagggcatt gaaaagctct tcgatgcggt cattctttcc
     4021 ttgcaggtac agccagccca gcagacattc aaagccggtg gaggcgcggt actcctgcac
     4081 ggtggcgtgc ttggccaccg tggccttgct ggcgtttttg ccgcgccgca gcacagcggc
     4141 ctcggcgtcg gtcagcatcg gctcgatgat gtccagctcc aaattctgcg cgtgggcgct
     4201 gacgtagtgg gtcgcgaccg tgtgcaggcg gttgggttgc agccgggtcg tgcccaccag
     4261 gcgggcgcga accagcagtt ccagcacggc gtcgcccaca aaggccagcg ccagcggcga
     4321 catttcgtga atgtcgactt ccgatttttg tgtaaacaac atagttttca ctgcttagta
     4381 caggtaatca aactcgtact caccgatctt gatggtgtcg tcctcttcca cgtcctgcgc
     4441 gaccagctcg tccagaatgc cggaatcacc cagctgcgtc tggaaatact g
//
LOCUS       DBMDSQ010000159         4468 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_4609_length_4468_cov_1.726815, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000159 DBMDSQ010000000
VERSION     DBMDSQ010000159.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 4468)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4468)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4468
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4609_length_4468_cov_1.726815"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..842
                     /locus_tag="ACZPAX_06570"
     CDS             <1..842
                     /locus_tag="ACZPAX_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005927183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR1113598.1"
                     /translation="IGTWEAKELTTDMIITKMVGRELSNLYPPRSNTPGEVVFEVKDF
                     TSINPKSFRHVNFNVRKGEILGVAGLVGAQRTELMEGLFGLRCHTSGTITYKGKELTI
                     NQPQDAIKNGIAMLTEDRRATGILGVLSIADNVSIASLNKYLIGGIMLDDNKIEKLVQ
                     DNVAKLSIKTPSSKTQIQSLSGGNQQKVLIARWLANDPDVFIMDEPTRGIDVGAKYEI
                     YCIIAEMAKQGKSIIMISSEMSELIGMSDRIMVMCDGRCTGFIDGDKANQENIMTLAT
                     QFE"
     gene            857..1840
                     /locus_tag="ACZPAX_06575"
     CDS             857..1840
                     /locus_tag="ACZPAX_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015554198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HIR1113599.1"
                     /translation="MEKKFNFKKFLSNNAIIILIILLALFVGCTTQNFFTETNGKNLL
                     LNVAPRFIIACGVSGCLITKGTDLSAGRQVGLAACFSAMLLQSVDYSARMLPWLPDIP
                     WPVALVIVMAIMACFGAINGCIIAFLKVPPFIATLGMQTIVYGLCSVITNNQPMGGYK
                     QSYLTVASGTLGPIPFLAIFALIVGIFFWFLYNKTRHGKYMYAIGGNENAAQVAGVNV
                     TASLIRIYILASCMYALGGFLVGAKAGGASTNTGNGYELEAIAACTIGGVSTNGGVGK
                     VSGVLVGVLVFEVLKICLQFLGVDPAYTYIAQGLVIVIAVALDLRKYLAKK"
     gene            1844..2044
                     /locus_tag="ACZPAX_06580"
     CDS             1844..2044
                     /locus_tag="ACZPAX_06580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113600.1"
                     /translation="MGEEKTTPETEKEPDRPDRSQLNAFENFYEKFRKVPLKYIDIFI
                     GVCVAALVIVVLVGILKSKGIL"
     gene            complement(2200..2778)
                     /locus_tag="ACZPAX_06585"
     CDS             complement(2200..2778)
                     /locus_tag="ACZPAX_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005924945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HIR1113601.1"
                     /translation="MSRQVWKGSTLLNPEPPVLVSCGSPDKPNLITVGWTGTICTQPP
                     MLSISVRPERYSHHLIQESGEFVVNLPTESLVRAIDWCGVKSGRDVDKFAAMHLTAAP
                     AAKVGTVLVEESPVNLECKVTQVISLGSHDLFLAECVAVDVDERLLDESGKLCLNKAK
                     LIVYSHGDYLALGRKLGSFGYSVRKKKKAVKK"
     gene            complement(2775..3761)
                     /locus_tag="ACZPAX_06590"
     CDS             complement(2775..3761)
                     /locus_tag="ACZPAX_06590"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR1113602.1"
                     /translation="MNNRTEPILYIVIPCYNEQEVLPITAPMFLQKINDLAAAGKISP
                     DSRVLFVNDGSKDRTWEIINELAASDEHYVGICQSRNRGHQNAVLAGLMEAKSRCDIT
                     ISIDCDGQDDINAMDGMVDAYRDGCDIVYGVRSKRDTDTFFKRFTAESFYKILNAMGA
                     EVVFNHADYRLMSARALEEFAKFKEVNLFLRGMVPLVGFKSTCVTYERHERIAGESHY
                     PLSKMLALAFDGITSLSIKPIRFITGFGVFVALVSFIGVLWAVIEAAMGLTVSGWASM
                     TSIICFVSGVQLICLGVIGEYIGKIYLESKHRPRYIISETTPNFGEAKEDAQ"
     gene            complement(3796..>4468)
                     /locus_tag="ACZPAX_06595"
     CDS             complement(3796..>4468)
                     /locus_tag="ACZPAX_06595"
                     /inference="COORDINATES: protein motif:HMM:NF012257.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIR1113603.1"
                     /translation="ETMRRTRLLRGMTDAELDTLMTCFAPRVRRYAKGELLLMAGYET
                     REVGLVLEGSITASKPLADGSTVVISHMGPGGVFADVLAGGRSRSPVNVAAAEPCLAL
                     YLPCEALLRPCAALHSAHLKLLQNWLETISDKYFALDRRLELICCKSLRGRICMWLLE
                     QRERCGADTFTTSMNRTELAAFLNCDRSALSRELSRMQEEGLIELFRGSFKLPDPERL
                     RAACP"
BASE COUNT          940 a         1371 c         1221 g          936 t
ORIGIN      
        1 acatcggcac ctgggaggcc aaggaactga ccaccgatat gatcatcacc aagatggtcg
       61 gacgtgagct gtcgaacctc tacccgccgc gctcgaatac acccggtgag gtcgtgtttg
      121 aggtcaagga cttcacatcc atcaacccca agagcttccg tcacgtcaac ttcaacgtcc
      181 gcaagggcga gatcctcggc gttgcgggtc tggtcggtgc ccagcgcacc gagctgatgg
      241 aaggtctgtt cggtctgcgc tgccacacgt cgggcaccat cacctataag ggcaaggaac
      301 tgaccatcaa ccagccgcag gacgccatca aaaacggcat cgccatgctg accgaggacc
      361 gccgcgcgac cggtattctg ggtgtgcttt ccattgcgga taacgtctcc attgcatcgc
      421 tgaacaagta tctgatcggc ggcatcatgc tggacgacaa caaaatcgaa aagctcgtgc
      481 aggataatgt cgccaagctg tccatcaaga cgccgtcctc caaaacgcag attcaatcgc
      541 tgtccggcgg taaccagcag aaggtgctga tcgcccgctg gctggccaac gaccccgatg
      601 ttttcatcat ggacgagccg acccgcggta tcgacgtcgg tgcaaagtat gaaatttact
      661 gcatcatcgc cgagatggcc aaacagggca agagcatcat catgatctcc tccgaaatga
      721 gcgagctgat cggtatgtct gaccgcatca tggtcatgtg cgatggccgc tgcaccggct
      781 ttatcgacgg tgataaggcg aaccaggaga acatcatgac gctggctacc cagtttgaat
      841 aaggaggaat ccgaaaatgg agaaaaaatt caactttaaa aaattcctgt ccaataacgc
      901 gatcattatt ctgatcattt tgctggccct cttcgtcggc tgcacgaccc agaacttctt
      961 taccgagaca aacggcaaaa acctgctgct gaacgtcgcc ccgcgtttca tcatcgcctg
     1021 cggcgtttcg ggatgcctga ttaccaaggg cactgacctg tccgccggcc gtcaggtcgg
     1081 cctggccgcc tgcttctccg ccatgctgct gcagagcgtt gactactctg cccgcatgct
     1141 gccttggctg cccgatattc cgtggcccgt ggccctcgtc atcgtcatgg cgatcatggc
     1201 ctgcttcggt gccatcaacg gctgcattat cgcattcctg aaggttcctc ctttcatcgc
     1261 tacgctgggt atgcagacca tcgtctacgg cctgtgctcc gtcatcacga acaaccagcc
     1321 catgggcggt tacaagcaga gctacctgac cgttgccagc ggcacgctgg gccccatccc
     1381 gttcctggcc atctttgcac tgatcgtcgg catcttcttc tggttcctct acaataagac
     1441 ccgtcacggc aagtacatgt acgccatcgg cggcaacgag aacgccgctc aggttgccgg
     1501 tgtcaacgtc accgcgtccc tgatccgcat ctacatcctg gcctcctgca tgtatgctct
     1561 cggtggcttc ctcgtcggcg caaaggccgg cggcgcttcc accaacaccg gcaacggcta
     1621 tgagctggaa gctatcgcag cctgcaccat cggcggcgtt tccaccaacg gcggcgtcgg
     1681 caaggtctcc ggcgttctgg tcggtgttct ggtctttgaa gtcctgaaga tttgcctgca
     1741 gttcctcggc gtcgaccccg cctacaccta cattgcacag ggccttgtca tcgtcatcgc
     1801 cgttgccctt gacctgcgca agtacctggc gaagaagtaa cctatgggcg aagaaaagac
     1861 tactcccgag accgaaaagg aacccgaccg ccctgaccgc agccagctga atgcctttga
     1921 gaatttctac gagaaattcc gcaaggttcc gctgaaatac atagacatct tcatcggcgt
     1981 ctgcgtagcc gccctggtca tcgtagtgct ggtcggcatc ctcaagagta agggaatatt
     2041 gtaaaaaaag aagcaccgtt cacggagaaa aatctctgtg aacggtgttt ttatttgtgc
     2101 tttagcagag ttgtaggggc cgcacatgtg cggcccgcag ccttgcctat acagcgcggc
     2161 ggccttctag ccgagaggcg gatatggaat ttgcccctat tattttttga ccgccttttt
     2221 cttcttcctg acactatacc caaagctgcc cagtttgcgc cccagcgcca ggtaatctcc
     2281 atggctgtac acaatcagct ttgccttgtt caagcacaat ttcccgcttt catccagcag
     2341 ccgctcgtcc acatctacgg cgacgcactc cgccaagaac aagtcatggc tgcctaaaga
     2401 aatgacctgc gtgaccttgc actccaaatt caccgggctt tcctcgacca aaaccgtgcc
     2461 caccttggcg gcgggggcgg cggtcaggtg catggcggca aacttgtcca cgtcgcggcc
     2521 gctcttgacg ccgcaccagt cgatggcccg gactaagctc tcggtgggca ggttcacgac
     2581 gaactcgccg ctttcttgaa ttaaatggtg gctgtaccgc tcgggccgca cactgatgga
     2641 cagcatcggc ggctgggtgc agatggtgcc ggtccagccc acggtgataa ggttcggctt
     2701 gtcggggctg ccgcagctga ccaacaccgg cggctccggg ttcagcaggg tagacccctt
     2761 ccatacctgg cggctcattg tgcatcctcc ttggcttcac caaaattcgg cgtcgtttcg
     2821 ctgataatat agcgcgggcg gtgcttggat tccaggtaga ttttgccaat gtactcaccg
     2881 atcacgccca ggcaaatcag ctgcacgcca gacacaaagc agatgatgct cgtcatcgat
     2941 gcccagccgg aaacagtcag gcccatggcg gcctcaatga cagcccacag cacgccgatg
     3001 aagctgacca gcgccacaaa cacgccaaag ccggtgatga accgaatcgg cttgatggac
     3061 aaactcgtga tgccgtcgaa agccagcgcc agcattttgg acaggggata gtggctctcg
     3121 cccgcaatgc gctcgtggcg ttcgtaggtc acgcaggtgg atttaaagcc caccagcggc
     3181 accatgccgc gcaggaacag gttgacctct ttgaatttcg caaactcttc cagtgcgcgg
     3241 gcgctcatca ggcggtagtc tgcgtggtta aagacaactt ccgcacccat ggcattcaag
     3301 attttgtaga aactctcggc ggtaaaacgc ttgaaaaacg tgtcggtatc ccgcttgctg
     3361 cgcacgccgt agacgatatc gcaaccgtcg cggtaggcat ccaccatgcc gtccatggcg
     3421 ttgatgtcgt cctggccgtc gcagtcgatg gaaatggtga tgtcgcagcg gctctttgcc
     3481 tccatcaagc ccgccagcac ggcgttctgg tggccgcggt tgcggctttg gcagatgccg
     3541 acgtaatgct cgtcactggc ggccaattcg ttgatgattt cccaggtgcg gtcctttgag
     3601 ccgtcgttca caaacagcac acggctgtcc gggctgatct tgcccgccgc ggccagatcg
     3661 ttaattttct gcaaaaacat cggtgcagtg atgggtaaaa cctcctgctc gttgtagcag
     3721 gggatgacga tgtacaaaat cggttcagtg cggttgttca tacaaataac ctcgatgtct
     3781 ttattttatg ggtacttatg ggcaggcggc ccgcagccgc tcgggatccg gcagtttaaa
     3841 gctgccccgg aacagctcga tcagcccttc ctcctgcata cggctcaact cgcgggaaag
     3901 cgcactgcgg tcgcagttca aaaaggcagc cagctcggtg cggttcatgc tggtggtaaa
     3961 ggtgtccgcg ccgcagcgct cccgctgttc cagcagccac atacagatgc gcccacgcag
     4021 gctcttacag caaatcagct ccaaacggcg gtccagcgca aaatatttgt cactgatggt
     4081 ttccagccag ttctgtagca gcttcaaatg ggcgctgtgc agcgcggcgc aggggcgcag
     4141 caacgcctcg cagggcaggt agagcgccag gcacggctcg gcagcagcta cattgactgg
     4201 gctgcggctg cgcccaccgg ccaaaacatc ggcaaacaca ccgcccggcc ccatgtggga
     4261 gataacgacc gtgctgccgt cggccagcgg cttgctggcg gtgatggaac cttccagcac
     4321 caagccgact tcccgcgtct cgtacccggc catcagcagc agctcgccct tggcatagcg
     4381 gcgcacccgg ggcgcaaagc aggtcatcag cgtgtccagc tcggcgtcgg tcatcccgcg
     4441 caaaaggcgg gtgcggcgca tcgtctcg
//
LOCUS       DBMDSQ010000160         4457 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_4619_length_4457_cov_2.408861, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000160 DBMDSQ010000000
VERSION     DBMDSQ010000160.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 4457)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4457)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4457
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4619_length_4457_cov_2.408861"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            55..252
                     /locus_tag="ACZPAX_06600"
     CDS             55..252
                     /locus_tag="ACZPAX_06600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113604.1"
                     /translation="MGTDAVANLIREGKCYQIPSVLQSGAALGMHSLNADLARLVSLG
                     RITRETAERCATDKSDLRNYL"
     gene            complement(302..745)
                     /locus_tag="ACZPAX_06605"
     CDS             complement(302..745)
                     /locus_tag="ACZPAX_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HIR1113605.1"
                     /translation="MEERNVFLWVKAAVCTACGAFTAAFGWLGWLIAAWAACMALDWL
                     SGSAAAASRGQWSSAAARAGIWHKAGMLVVVLVSALTDAVLGMAAANLPGLELPFDGV
                     VLPVVLVWYIFTELGSIAENAAAMGAPVPGCLVKILAEGKEKAGK"
     gene            complement(766..1374)
                     /locus_tag="ACZPAX_06610"
     CDS             complement(766..1374)
                     /locus_tag="ACZPAX_06610"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007048324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HIR1113606.1"
                     /translation="MRQTGIFAGRTQVVYPYGRYGWTRGGGKIWHGGLDLVGLDDTTV
                     RMPYYKGKRITGRVVRARIVTDRRDKTWEWGWYVCVQLDSAQTPDVVNFLYFAHCEKL
                     LAQAGQTVTSGDALAVMGNTGNAAGGYKHCHLEVRATAGGTGLDPTAYAGCENAVGIY
                     GAAAKLQTITIGPVSQGDADAVKALCDARGLTAAGLYTSKWA"
     gene            complement(1371..2219)
                     /locus_tag="ACZPAX_06615"
     CDS             complement(1371..2219)
                     /locus_tag="ACZPAX_06615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113607.1"
                     /translation="MDLKAIQAALLFGGQSGPGGDWAEAVCAPFTASGGTVVCSPLAG
                     RALTVAAAGATTQAGGGTAAPGNIRALGGVGKNGSLTLTAAGGETREVEIPLTGPLYT
                     GDMVALAADGSVTETHRAIKLTVDGSENWISWTNPRDTADITVPGTIGSLGDILCSHY
                     SPLAGTLSSDAGLYTVSTNTTAEPGKTRIILRHGFKTLALFKEYLKAQADAGTPVTFV
                     YKLTPATETPTVTKRTAEPLCAVPDADGLCTVQGGGTLTVTGYEDPRATRRALEKRIA
                     ALEAKA"
     gene            complement(2243..2461)
                     /locus_tag="ACZPAX_06620"
     CDS             complement(2243..2461)
                     /locus_tag="ACZPAX_06620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113608.1"
                     /translation="MKLIEETWKTGTAYEVLLVSDKAPAALPASGKDVDGMNDAHTFA
                     PMSVLYVVDPAAAHKFYVADESGRFVGQ"
     gene            complement(2458..3915)
                     /locus_tag="ACZPAX_06625"
     CDS             complement(2458..3915)
                     /locus_tag="ACZPAX_06625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113609.1"
                     /translation="MKLHGHARLELTDVHTGEVEVVESDNLITNAVSDIFNGYGSSLN
                     KAMLLWRGDTGYTDAPKDLVSMFYGGLLLYDTALGAEPGTLFAPAAAGVVGTARCNVV
                     NTTKNTTRGSANLTETNIDPAGGVVSYVYEFATNQANGIIRSVCLTHPMGALYSEMTE
                     APDASPTNIDRLAFGGALGGDQLLTGLLATNCYPTNEKGVLLYADPAADEAVAGRVDV
                     ENAALELRHHCLGLHTLDLFRRDTAGTWDSLLRTETLDITDFLQDVSANAGRYCRLCF
                     DAEADKLYLVSIPAGSSIAVGASIKVREYDRKTLAAKDYTVPNNTGGTLRGLVSESRA
                     TPLNGTVYGGSLYMTDPATYQIYRFPLSDPTDVQAVTMHSDYMQPLCDAHNGRLYCYY
                     RTRGVVLNTEKNELFSCEAAGYDTASSGFSTTVPVLGEPAAPLRRYLSDNRIRTERVL
                     RANYLATINDLPKAVEKTADKTMKVIYTLRKGEDA"
     gene            complement(3912..>4457)
                     /locus_tag="ACZPAX_06630"
     CDS             complement(3912..>4457)
                     /locus_tag="ACZPAX_06630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113610.1"
                     /translation="NPYLTNNSDGTTEKLLRKVQNSAESKRLVYYSFTNTAALTVRTA
                     ETPAVSIAFAAVEDTSAMFLAQLLLTAEPEGGDPLTLEVRYYVNEVRVENFTPQQRLL
                     AGAHTLALFYPFASVEANAAKRLSVRLVCTGGKVKIAPYSIKATVTGQGMASELPWDG
                     TLQFEELLMPLQLTERKVILE"
BASE COUNT          709 a         1529 c         1357 g          862 t
ORIGIN      
        1 ctcgggatct ggtagcactt gccctcgcgg atcaggttcg ccacggaaat tcttgtgggc
       61 accgacgccg tggcgaacct gatccgcgag ggcaagtgct accagatccc gagcgttttg
      121 caatccggcg cggcgctggg tatgcacagc ttgaacgctg acctggcccg cctggtcagc
      181 ctgggccgca tcacccgcga aaccgccgag cgctgcgcga cagacaagag cgatctgcgg
      241 aattatttgt aatagcaacc gaaaaaaggg tatctgccga ttcgacagat acccttttgt
      301 tttatttccc cgccttctcc tttccttccg ccaagatctt taccaggcag cccggcaccg
      361 gggcgcccat ggcggcggcg ttctcggcaa tggagcctag ctcggtaaaa atataccaca
      421 ccagcactac cggcagcaca acgccgtcaa agggcagttc cagccccggc aggttggcgg
      481 cggccatgcc cagcaccgca tcggtcaacg cgctgaccag cacgaccacc agcattccgg
      541 ccttgtgcca gatgcccgcc cgcgccgctg cgctggacca ctgcccccgg ctggccgcag
      601 cggcactgcc gctgagccaa tccagcgcca tgcaggccgc ccacgctgcg atgagccacc
      661 ccagccagcc gaaggccgcc gtaaacgccc cgcaggcggt gcagaccgcc gccttgaccc
      721 acaaaaacac attgcgttct tccactcctt gctcctttcc gggcgttacg cccacttgct
      781 tgtgtacagc cccgccgccg tcagaccccg ggcgtcgcac agggctttca ccgcgtcggc
      841 gtcaccctgg ctgacggggc cgatcgtgat ggtttgcagc tttgccgccg cgccgtagat
      901 tcccaccgcg ttctcacacc cggcgtaggc cgtggggtcc agcccggtgc cgcccgctgt
      961 ggcccgcacc tccagatggc agtgcttgta gccgcctgcc gcattgccgg tattgcccat
     1021 cacagccagc gcgtcgccgc tggtgaccgt ttggcccgcc tgcgccagca gtttttcaca
     1081 gtgggcaaag tacaaaaagt tcacgacgtc gggcgtctgg gcgctgtcca gctgcacgca
     1141 gacataccag ccccactccc aggttttgtc gcggcggtcg gtcacaatgc gcgcccgcac
     1201 cacgcggccg gtaatgcgct tacccttata atagggcatc cgcacggtgg tgtcgtccag
     1261 tcccaccagg tccagcccgc cgtgccagat tttgccgccg ccccgggtcc agccgtagcg
     1321 gccatagggg tacaccacct gggtgcggcc cgcaaaaata ccggtctgcc tcacgccttc
     1381 gcctccaaag cggcaatgcg cttttccagg gcacggcggg tggcgcgggg atcctcgtac
     1441 ccggtcacgg tcagggtgcc cccgccctgc acggtacaca ggccgtcggc atccggtacg
     1501 gcgcacagcg gttcggcggt gcgttttgtc accgtcggcg tttcggtggc gggcgtcagc
     1561 ttgtacacaa acgtcaccgg tgtgcccgcg tcggcctggg ctttcaggta ttctttgaac
     1621 agcgccagcg tcttgaagcc gtggcgcagg atgatccgcg ttttgcccgg ctcggcggtt
     1681 gtgttggtgc tgaccgtgta cagccccgcg tccgaggaca gcgtgcccgc cagcgggctg
     1741 taatggctgc acaggatgtc ccccaggctg ccgatggtgc cgggcacggt gatatccgcc
     1801 gtgtcccgcg ggttcgtcca ggagatccag ttctcgctgc cgtccacggt caattttatt
     1861 gcgcggtgcg tctcggtcac gctgccgtct gccgccagcg ccaccatgtc cccggtgtac
     1921 agcggtccgg tcagcgggat ttccacttcc cgcgtctcgc cgcctgcggc ggtcagggtc
     1981 aggctgccgt tcttccccac gccgcccaac gcgcggatgt tcccgggggc ggcggtgccc
     2041 ccgcccgcct gggtcgtcgc gcccgccgcc gccacggtca gggcacgccc ggccagcggg
     2101 ctgcaaacga ccgtcccgcc gctggcggtg aacggcgcgc agacagcctc ggcccagtcg
     2161 ccgcccgggc cgctttggcc gccaaacagc agcgcggcct gtatcgcttt caaatccatg
     2221 ttacacactc ctttttcggg ctttattggc ccacgaaccg gccggactcg tcggccacgt
     2281 agaacttatg cgctgccgcc gggtccacca catacaaaac cgacatcggt gcaaaggtgt
     2341 gggcgtcgtt catgccgtcc acgtccttgc cgctggcggg cagtgctgcc ggggccttgt
     2401 cggacaccag cagcacctca taggcggtgc cggttttcca ggtttcttcg atcagtttca
     2461 tgcgtcttct cctttccgca gggtgtagat tactttcatc gtcttatcgg cggttttctc
     2521 caccgccttg ggcaggtcgt tgatggtggc caggtagttg gcccgcagca cccgttccgt
     2581 gcggatgcgg ttgtcgctca aatagcggcg cagcggtgcg gcaggctccc ccagcaccgg
     2641 cacggtggtc gagaaccccg aactggccgt gtcatacccc gccgcctcgc aggagaacag
     2701 ctcgttcttc tcggtgttca gcacgacgcc gcgggtgcgg tagtaacagt agagccgccc
     2761 gttgtgggcg tcgcacagcg gctgcatata atcgctgtgc atcgtgacgg cctgcacgtc
     2821 ggtgggatcg ctaagcggga agcggtagat ctggtaggtc gccggatccg tcatgtacag
     2881 gctgccgccg tagaccgtac cgttcagcgg cgtggcccgg ctctcgctga ccaggccgcg
     2941 cagcgtgccg ccggtgttgt tgggcacggt atagtccttg gcggccagcg tctttcggtc
     3001 gtactcccgc actttgatac tggcccccac cgcaatgctg ctgcccgccg ggatggagac
     3061 aagatacaac ttgtcggcct cggcgtcaaa gcaaagacgg cagtaccgcc cggcgttggc
     3121 cgaaacatcc tgcaaaaagt cggtgatatc cagcgtctcg gtgcgcagca ggctgtccca
     3181 ggtgcccgcc gtatcgcggc ggaagaggtc cagggtatgc agccccaggc agtggtggcg
     3241 tagttccagc gcagcgttct ccacatccac ccggcccgcc acggcctcat ctgccgccgg
     3301 gtccgcgtag agcagcaccc ccttctcgtt cgtgggatag caattggtcg ccagcagccc
     3361 ggtcagcagc tggtcgccgc ccagtgcccc gccgaaggcc agccgatcaa tgttcgtcgg
     3421 gctggcgtcg ggagcctccg tcatctcgga gtagagtgct cccatcgggt gggtcaggca
     3481 gaccgagcgg atgatgccgt tggcctggtt cgtggcaaac tcatagacgt agctcaccac
     3541 accgcccgcc gggtcgatgt tcgtctcggt caggttggca ctgccgcggg tcgtgttttt
     3601 ggtggtgttg acgacattgc accgcgccgt gcccaccacg cccgcagccg ccggggcaaa
     3661 cagggtgccc ggctcggccc ccagcgcggt atcgtacaaa agcagcccgc cgtaaaacat
     3721 cgagaccaaa tccttcggtg cgtcggtgta gccggtatca ccgcgccaca gcagcatggc
     3781 cttgttcagg ctgctgccgt agccgttgaa aatatcgctc accgcgttcg taataaggtt
     3841 gtcgctctcc accacctcta cttccccggt gtgcacatcc gtcagttcca ggcgggcgtg
     3901 gccgtgcagt ttcattccaa aatcaccttt ctttctgtca gttgcagcgg catcagcagt
     3961 tcctcaaatt gcagcgtgcc gtcccagggc agctcgctgg ccatgccttg gccggtcacg
     4021 gtggccttga tgctgtaggg cgcaattttt accttgccgc cggtgcagac cagccgcacc
     4081 gaaagccgct tggccgcatt ggcttcaaca ctggcgaagg ggtaaaacag cgccagcgtg
     4141 tgcgcccccg ccaaaagccg ctgctgcggg gtaaagtttt ccacccgcac ttcatttaca
     4201 tagtaccgca cttccagtgt cagcgggtcg ccgccctccg gctcggcggt cagcagcagc
     4261 tgggccagga acatggcgct cgtgtcctcc accgccgcaa aggcgatgga cacagccgga
     4321 gtctccgctg tgcgcacggt cagcgccgcc gtgttcgtaa aactgtaata caccagccgt
     4381 ttgctctcgg cgctgttctg cactttgcgc agcagctttt ccgtcgtacc atcgctgttg
     4441 ttggtcaagt aagggtt
//
LOCUS       DBMDSQ010000161         4427 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_4655_length_4427_cov_1.969718, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000161 DBMDSQ010000000
VERSION     DBMDSQ010000161.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 4427)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4427)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4427
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4655_length_4427_cov_1.969718"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..523)
                     /locus_tag="ACZPAX_06635"
     CDS             complement(<1..523)
                     /locus_tag="ACZPAX_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015565409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1113611.1"
                     /translation="MASISCRHVKKVYPGNVTAVPDFSLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGEMYIGDRLINDVPPKDRDIAMVFQNYALYPHMTVYKNMAFGLE
                     LRKMPKDEIDKRVREAAKILEIEHLLDRKPKALSGGQRQRVALGRAMVRNPAVFLLDE
                     PLSNLDAKLRTSMR"
     gene            644..1426
                     /locus_tag="ACZPAX_06640"
     CDS             644..1426
                     /locus_tag="ACZPAX_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005923096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1113612.1"
                     /translation="MPKLIFDPEIYCNAAENAELDGSTPLTLAGNGLWVGVLSRGECL
                     LDGFDRPGQSGDILLGPAPLTLTPQTDCHLLAVRLTGHCTDAYLLQLGAARWADGAAC
                     PGAAELIAQLHGIHTAAMAYALLCAVGKADETARPLPPLVAEAVAAIRQNYMALYGVE
                     ELSEQLGVTKSHLVRVFKQEIGIPPGKYMTNVRIEAVKALLLSDEYNLDMIAGLTGFS
                     GANYLCRVFKREVGLSPAAWRAAAAPLPAVPKLPPRSQEMYV"
     gene            complement(1591..2616)
                     /gene="galE"
                     /locus_tag="ACZPAX_06645"
     CDS             complement(1591..2616)
                     /gene="galE"
                     /locus_tag="ACZPAX_06645"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005937424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HIR1113613.1"
                     /translation="MAILVSGGAGYIGSHTCVELLAAGYDIVVADNYYNSCPEALARV
                     KKIAGKDFRFVEADMTDKAAVEKIFAENEDIDCVIQFAAYKAVGESVSKPIEYYSNNL
                     ACTLNILDVMRRHNCHNIIFSSSATVYGDPASVPIREDFPVGGTTNPYGTTKVFTEQI
                     LTDCCKADPELNVALLRYFNPIGAHPSGLIGEDPNGIPNNLVPYIAKVAVGKLEKVHV
                     FGNDYPTPDGTGVRDYIHVVDLARGHVAAIKKLEEKPGLFICNLGTGHGYSVLDVIHA
                     FSKACGKEIPYVIDPRRPGDIAECWCDPSKAKRELGWEAQYGIEEMCAHSWNWQSHNP
                     DGYKTAK"
     gene            complement(2688..3620)
                     /locus_tag="ACZPAX_06650"
     CDS             complement(2688..3620)
                     /locus_tag="ACZPAX_06650"
                     /inference="COORDINATES: protein motif:HMM:NF024242.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1113614.1"
                     /translation="MLNDDFPLRVEAVPAAHTTEWRADDSFLPSETEAWQLIFVRSGT
                     IEERCDDRHVLLRAGGLLFHQPGEVFAMTTVGEVPPETLRIDFYCTGAAMDRFRGAML
                     HAEPAEQHDLNWLANAANELFDAAPVPGGRPVVKEELPFGAMQQFIIHLENLLILLAR
                     RCRRLRRPVARVRRERRQNALVEDARAYFVENLERELKVDEVCEALGCTRPQLQQAFR
                     ARLRRTAMEEFSAMRIEYAAQLLARGASPGEVAAQMGYCSGAYFSQKFRAATGHTPSA
                     YRRIQQGLPAKRQNRQQKDKAESKTSIAESPQKK"
     gene            complement(3630..>4427)
                     /locus_tag="ACZPAX_06655"
     CDS             complement(3630..>4427)
                     /locus_tag="ACZPAX_06655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113615.1"
                     /translation="SVFLNDAAHGLTWDEADRTAAVQQTRMAVDWITAQAESYGVMPD
                     LICDRTEDGSNAALTRSYLVQSAMRGGEDSDESTAFIEEMDALCESLAADSRLAVYGT
                     RHIGFLFYLPISGTSFTMAHYADDGEYFYYEYSCLYRHDAYTDGERESPATYAHEILH
                     LFGAPDLYEGSGDPYVDRALIDYVAETYPDDIMVSTYEDDGTSRFDVITKEISPLTAY
                     CLGLTDTCPELEQFPLLTQVEPGVFRRELTADTSDETADAWPGAVAA"
BASE COUNT          798 a         1320 c         1411 g          898 t
ORIGIN      
        1 tacgcatgct ggtacgcagc ttagcatcca ggttggacag aggctcgtcg agcaggaaga
       61 ccgccgggtt acggaccatt gcacggccca gggcaacacg ctgacgctga ccaccagaca
      121 gagccttcgg cttacggtcg agcaggtgct caatctccag gatcttagca gcctcgcgga
      181 cgcgcttgtc gatttcgtcc ttgggcatct tgcgcagctc cagaccgaaa gccatgttct
      241 tgtaaacggt catgtgcggg tacagagcgt agttctggaa gaccatcgcg atgtcgcggt
      301 ctttcggggg aacgtcgttg atcagacggt cgccgatgta catctcgccg ccggagatct
      361 cctccaggcc agcgatcata cgcagggtgg tggacttacc acagccggaa ggaccgacga
      421 aaacgataaa ctccttatcc gcgatttcca ggctgaaatc gggaacagcg gtaacattgc
      481 cggggtaaac tttcttgacg tgacggcagc taatggaagc cataatgctc atcctccata
      541 aaattcagtg gtattcagcc catgtagagg gggctgtttc tgttgacatg attataataa
      601 caggttataa tctgtttgaa aatagcattt tattgatatc gaggtgccaa aattgatatt
      661 tgacccagaa atttactgca atgctgccga gaacgccgag ctggacggca gtacccccct
      721 gaccctggcc gggaacggcc tgtgggtggg ggtactctcc cgcggggaat gcctgctgga
      781 cggctttgac cgcccaggcc agagcggcga tatcctgctg gggcctgccc ccctgacgct
      841 gacgccccaa acggactgcc acctgctggc tgtgcggctg accggccact gcacggacgc
      901 ctacctatta cagttgggtg cggcccgctg ggctgacggc gcggcctgcc ccggcgcggc
      961 cgagctgatt gcccagctgc acggtatcca cacggcggcg atggcctatg cgctgctctg
     1021 cgccgtgggc aaggctgacg agacggcccg tcccctgccc ccgctggtgg ctgaggctgt
     1081 ggcggccatc cggcagaact acatggccct ctacggcgtg gaggaactga gcgagcagct
     1141 cggcgtgacg aagagccatc tggtgcgggt gttcaagcag gaaatcggta tcccacccgg
     1201 aaagtacatg acgaatgtgc gcatcgaggc cgtgaaggcg ttgctgctca gcgatgagta
     1261 taacctggat atgattgcag gcctgaccgg gttttccggc gcaaactacc tctgccgcgt
     1321 gttcaagcgg gaggtgggcc tctcccctgc cgcctggcgt gccgccgctg caccgctgcc
     1381 cgccgtgccc aaactgccgc cgcgcagtca ggaaatgtat gtgtaaaaga atatggcagg
     1441 agcagtaggg gccggacatg tccggcccgc agccttcccg caagcttccg tttgccatac
     1501 acacccgggc cgggcatgcc cggcccctac tgtgcgatga cataaaaaac ctcccaatgc
     1561 aagagcatca ggaggttttt ttatgcagtt ttatttcgcg gtcttatacc catcggggtt
     1621 gtggctctgc cagttccagc tgtgggcgca catttcctca atgccgtact gggcctccca
     1681 gcccagctcg cgctttgcct tgctggggtc gcaccagcac tcggcaatgt cgccggggcg
     1741 gcgcgggtcg atgacgtagg gaatctcttt gccgcaggcc ttgctgaagg catggatgac
     1801 gtccagcacg ctgtaaccgt ggccggtgcc caggttgcag atgaacaggc cgggcttctc
     1861 ttccagcttc ttgatggctg ccacatggcc gcgggccagg tcaacgacat ggatgtagtc
     1921 acgcacgccg gtgccgtcgg gcgtcgggta gtcgttgcca aagacatgga ccttctcgag
     1981 cttgccgaca gcgaccttgg cgatgtaagg caccaggttg ttgggaatgc cgttgggatc
     2041 ctcgccaatc aggccggacg gatgggcacc gatggggttg aagtaacgca gcagcgcaac
     2101 attcaactcg ggatccgcct tgcagcagtc ggtcaggatc tgctcggtga acaccttggt
     2161 tgtgccgtag gggttcgtgg tgccgcccac ggggaaatcc tcgcggatgg gcacgctggc
     2221 ggggtcgccg tagaccgtgg cagaggaaga gaagatgatg ttgtggcagt tgtggcggcg
     2281 catgacatcc aggatgttca gcgtgcaagc caggttgttg gagtagtact cgatgggctt
     2341 ggaaacgctc tcgcccacgg ccttgtaggc ggcgaactgg atgacgcagt caatgtcctc
     2401 gttctcagca aaaatcttct caacggcggc cttgtcggtc atgtcggcct cgacaaagcg
     2461 gaagtccttg ccggcaatct tcttgacgcg ggccagggcc tcggggcagg agttgtagta
     2521 gttatcggcc acgacgatgt cgtaccctgc ggccagaagt tccacacagg tgtggctgcc
     2581 gatgtaaccg gcgccaccgc taaccaaaat tgccataaca gtcgtctcct tatatttatg
     2641 cttttgttgc ctgtgggcgg gcgttctgcc tgccctttgt cactatttta ctttttctgc
     2701 ggtgattctg caatagaggt tttagattct gctttatctt tttgttgcct gttttgccgc
     2761 ttagcgggca gcccctgctg gatgcgccgg taggccgacg gtgtgtggcc tgtggctgcg
     2821 cggaatttct ggctgaaata cgcgcccgag cagtagccca tctgggccgc gacctcgccg
     2881 ggggatgccc cgcgtgccag cagctgggcc gcatactcga tgcgcatggc ggaaaattcc
     2941 tccatggccg tgcggcgcag ccgggcgcgg aaggcctgct gcagctgggg ccgggtgcag
     3001 cccagggcct cgcagacctc gtccactttc aactcccgtt ccaggttttc gacaaaatag
     3061 gcgcgggcat cctccaccag ggcattctgg cggcgctccc gccggacgcg ggccacgggg
     3121 cggcgcaggc ggcggcagcg ccgggccagc aggatcaaca ggttttccag gtggatgatg
     3181 aactgctgca tcgcgccgaa gggcagctcc tcttttacga cgggccgccc gcccggtacg
     3241 ggcgcggcgt cgaacagctc gttggcggcg ttggccagcc agttcaaatc gtgctgctcg
     3301 gcgggctcgg cgtggagcat cgcaccccgg aaccgatcca tggccgcgcc ggtgcagtaa
     3361 aaatcgatgc gcagcgtctc gggcgggacc tcccccaccg tcgtcatggc gaacacctcg
     3421 ccgggctggt ggaacagaag cccgcccgcc cgcagcagca catggcggtc gtcgcagcgt
     3481 tcctcaatgg tgccgctgcg cacaaaaatc agctgccaag cctcggtctc gctgggcaga
     3541 aaggaatcgt cggcgcgcca ttcggtggtg tgggcggcag gaaccgcctc gacccgcagc
     3601 ggaaaatcat cgttcagcat ggggcctcct tatgccgcca cggctcccgg ccaggcgtcg
     3661 gcggtttcgt cagatgtgtc tgccgtcaat tcacgccgga acacccctgg ctcgacctgg
     3721 gtcagcagcg ggaactgttc cagctccggg caggtgtcgg tcaggcccag gcagtaggcc
     3781 gtcagcgggc tgatctcctt cgtgattacg tcaaaccggc tcgtgccgtc gtcctcgtag
     3841 gtgcttacca tgatgtcgtc ggggtaagtc tcggccacgt agtctatcaa tgcgcggtcc
     3901 acataggggt cgccgctgcc ctcgtagagg tcaggtgctc cgaacaggtg caggatctcg
     3961 tgggcgtagg tcgccgggct ttcccgctca ccgtcggtgt aggcgtcgtg gcggtacagg
     4021 cagctgtact cgtagtagaa atactcgccg tcgtcggcat agtgtgccat tgtgaaggac
     4081 gtgccgctga tgggtaggta gaacagaaac ccgatgtgcc gcgtgccgta gaccgccagg
     4141 cggctgtccg cggccagcga ctcacacagg gcgtccattt cctcaatgaa ggcggtgctc
     4201 tcgtcggaat cctcgccccc gcgcatggca gactgcacaa gatagctgcg ggtcagggcg
     4261 gcgttgctgc catcctcggt gcggtcacag attaaatccg gcatcacgcc gtagctttcg
     4321 gcttgggctg tgatccaatc cacggccatc cgggtttgct gcacggcggc ggttcggtcg
     4381 gcctcgtccc aggtcaggcc gtgggcggcg tcgttcaaaa agacact
//
LOCUS       DBMDSQ010000162         4424 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_4659_length_4424_cov_1.989591, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000162 DBMDSQ010000000
VERSION     DBMDSQ010000162.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 4424)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4424)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4424
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4659_length_4424_cov_1.989591"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..540)
                     /locus_tag="ACZPAX_06660"
     CDS             complement(<1..540)
                     /locus_tag="ACZPAX_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PCRF domain-containing protein"
                     /protein_id="HIR1113616.1"
                     /translation="MITIDELKAKLGGMKTAVDDLRDALSIEASEKRLAELEHQMTMP
                     GFYDDQARSQKIIGEMGEVKNKLERFGKLATQYEEAETLLEMIEEENDPALAAEGEEA
                     VEGVEKSIDELQLMTMLNGEYDHNNAILTFHAGTGGTEAQDWAEMLYRMYTRWAEAHG
                     YSVEVLDVLDGDEAGIKSAS"
     gene            871..1887
                     /locus_tag="ACZPAX_06665"
     CDS             871..1887
                     /locus_tag="ACZPAX_06665"
                     /inference="COORDINATES: protein motif:HMM:NF022400.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RING finger protein"
                     /protein_id="HIR1113617.1"
                     /translation="MTRYTGNHCPVCEQAFTDEDDIVVCPDCGTPYHRACWQKVGVCM
                     HKSEHAAGFEWQPEVGPDADKAAHEATCPNCGTHNTPGAVRCAHCGCPLPKAPDPITA
                     KTDDEKSVPIYARDPSAVNNSRTAPEPHIDTFGTGEDGGIYRREIGPEDPIDGIKAKD
                     WAAYVGRSPMYYLMQFFRMSMTKKNATVCLSAFLFGPAYLFYRKMWKEGLLAALVMLL
                     LSAPELIAIVATFNPTLFGSLPLGWIPTAANICAVAAWAFKIVLGLFAVSWYRNDAKR
                     SIEKIYADYPEGDARTDALLQKGGTSFLSVILYYAVILLLASLVINLAGPDFVQYAMA
                     MSGY"
     gene            1941..2870
                     /locus_tag="ACZPAX_06670"
     CDS             1941..2870
                     /locus_tag="ACZPAX_06670"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HIR1113618.1"
                     /translation="MKTALVIMAAGIGSRFGGGIKQLAAVGPNGEIIMDYSIHDAIEA
                     GFDKIVFIIRHDIEDAFKEAIGDRIEKICAELGVEIDYAFQALENVPAGYAVPEGRSK
                     PWGTGQAVLACKGIIKEPFAVINADDYYGKEAFVQLHDFLQGYTPDKSGDLAMAGFVL
                     KNTLSDNGAVTRGICQMNDAHYLTGVLETSGIEKTADGAAAGGKSIDTDSLVSMNMWA
                     LTPEFVDLLDAGFVEFFEKAEPANPLKAEYLLPIYIDELLHAGKVSVKVLPTHDKWFG
                     VTYAEDKQTVIDSFAKLVADGGYKKDLFSDLKK"
     gene            complement(2991..3227)
                     /locus_tag="ACZPAX_06675"
     CDS             complement(2991..3227)
                     /locus_tag="ACZPAX_06675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HIR1113619.1"
                     /translation="MGGYKCDTCGHLESAKQLSGYANVKGVYTPQTTGAAQAAKASTI
                     PQTSDDMPIIPIVVISIAALLGLGVTAYLKKKQN"
     gene            3624..>4424
                     /locus_tag="ACZPAX_06680"
     CDS             3624..>4424
                     /locus_tag="ACZPAX_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HIR1113620.1"
                     /translation="MAKNELDITTLTPEQRDARLALDVERLLRFGRKHKLIKDLDVLV
                     ARNTLLDLLALAAPSEAKPPKEDPATPAALLDEMVELAAQKELFDGAVNQYRINFETR
                     LMGALMPRESEVCKKFKKLYQKQGAKAATDWFYQLCVDTNYIRTAQIAKNIQWNTATP
                     YGELEITINLTKPEKDPKTIALERLQPKSGYPACMLCKENIGYAGRINFPARQTHRIV
                     PITLAGEQFYLQYSPYAYFHEHCIMLHEQHKPMEMNKQTLAEIFDFVAQ"
BASE COUNT          959 a         1332 c         1252 g          881 t
ORIGIN      
        1 ggaggcggac ttgatgccgg cctcgtcgcc gtccagcacg tccagcacct caacgctgta
       61 cccgtgggct tcggcccagc gggtgtacat acggtagagc atttcggccc agtcctgggc
      121 ctcggtgccg ccagtgcctg cgtggaaggt caggatggcg ttgttgtggt cgtactcgcc
      181 gttcagcatc gtcatgagct gcagctcgtc gatggacttc tcgacgccct caacggcctc
      241 ctcaccctcg gcggccaggg cgggatcgtt ctcttcctcg atcatttcca gcagcgtctc
      301 ggcttcctca tactgagtgg ccagcttacc gaagcgctcg agcttgttct tcacttcgcc
      361 catctcaccg atgattttct ggctgcgggc ctggtcatcg tagaagccgg gcatcgtcat
      421 ctggtgttcc agctcagcaa ggcgcttctc gctggcctca atggacaggg cgtcgcgcag
      481 gtcgtccaca gcggttttca tgccgccgag ttttgctttc agttcgtcaa tggtaatcat
      541 atatggtatc cctcatcaca taagttggca taaaattaca tagtactatt ataagcgaat
      601 tagcgggcgt tgtaaagagg ggcagccgca taaaatattt gtaaaagagg gatgtatccc
      661 tcccggcaaa aacaacgccg ccatgtaggg gccgggtatg cccggcccgc agccgtcggg
      721 caaacgaccg cccggcccct acccggtggc gcaaggaaaa agggaaataa attcatttct
      781 cttgtaaagt ttttatgttt tctttgcatt tatccatttg atgcggtata atgtctttat
      841 gttactgaaa gaagaacgag gacacaccac atgacacgtt acaccggaaa ccactgccct
      901 gtctgcgaac aggcatttac cgacgaggac gatatcgtcg tttgccctga ctgcggcaca
      961 ccctaccacc gggcctgctg gcaaaaagtt ggcgtatgta tgcacaaatc cgagcacgcc
     1021 gccgggtttg agtggcagcc cgaggtcggc cccgacgccg acaaggccgc acacgaggcc
     1081 acctgcccca actgcggcac ccacaacacg ccgggcgctg tgcgctgtgc gcactgcggc
     1141 tgccccctgc ccaaagcgcc ggaccccatc accgccaaga cagacgatga aaagtctgtg
     1201 cccatctacg cccgcgaccc gtccgcggtg aacaacagcc gcaccgcgcc cgagccgcac
     1261 attgacacct tcggcaccgg cgaggacggc ggcatctacc gccgcgagat cggccccgag
     1321 gaccccatcg acggcatcaa ggccaaggac tgggccgcct acgtgggccg cagcccgatg
     1381 tactacctga tgcagttttt ccgcatgagc atgacgaaga agaatgccac ggtctgcctg
     1441 tcggcatttt tgttcggccc tgcgtacctt ttctaccgca agatgtggaa agagggtctg
     1501 ctggccgcac ttgtgatgct gctgctcagc gcgccggagc tgattgccat tgtggcgacg
     1561 ttcaacccga cgctgttcgg ctcgctgccg ctggggtgga tccccacggc ggccaacatc
     1621 tgcgccgtgg cggcgtgggc gttcaaaatc gtgctggggc tgtttgcggt atcgtggtac
     1681 cgcaacgatg ccaagcgcag cattgagaaa atttatgccg actaccccga gggcgacgcc
     1741 cgcaccgatg ccctgctgca aaagggcggc acgagctttt tgtcggtgat tttgtactac
     1801 gccgtcattc tgctgctggc gtccctggtc atcaacttgg ccggccccga ttttgtgcag
     1861 tatgccatgg cgatgagtgg gtattgatgg caccgcaaaa caacgcaata caccaatggt
     1921 gttaaaatag gaggaaactt atgaaaaccg cattagttat tatggccgct ggtatcggct
     1981 cccgcttcgg cggcggcatc aagcagctgg cagccgtcgg ccccaacggc gagatcatca
     2041 tggactattc cattcatgac gccatcgagg ccgggtttga caaaatcgtc tttatcatcc
     2101 gccacgatat cgaggacgct ttcaaagagg ccatcggcga ccgcatcgag aaaatctgcg
     2161 ccgagctggg cgttgagatc gactacgcgt tccaggcgtt ggagaacgtt cccgccgggt
     2221 acgctgtgcc cgagggccgc agcaagccct ggggcaccgg ccaggctgta ctggcctgca
     2281 agggcatcat caaggagccg ttcgccgtta tcaacgccga cgactactac ggcaaagagg
     2341 cttttgtcca gctgcacgac tttttgcagg gctacacgcc cgacaagtcc ggcgacctgg
     2401 ccatggcggg ctttgtgctg aaaaacaccc tgagcgacaa cggcgctgtc acccgcggca
     2461 tctgccagat gaacgacgcc cactacctga ccggcgtgtt ggagaccagc ggcattgaaa
     2521 agaccgccga cggcgcagca gcgggcggca agagcatcga cacggacagc cttgtctcga
     2581 tgaatatgtg ggcactgacc ccggagtttg tggacctgct ggacgccggt tttgttgagt
     2641 tctttgagaa ggccgagcct gccaacccgc tgaaagccga gtacctgctg cccatctaca
     2701 ttgacgagct gctgcacgcg ggcaaggtca gtgtcaaggt cctgcccacc cacgacaagt
     2761 ggttcggcgt gacctacgcc gaggacaagc agaccgtcat tgacagcttc gccaagctgg
     2821 tcgccgatgg cgggtataag aaggatttgt tcagcgattt gaagaagtaa gaaagcaaag
     2881 cctccctttg acaagggagg ctttaggctt cccccttggg ggaaggcaaa accgccccat
     2941 gcaccatcac ggtgcatggg gcggtttgct tttgctattt cagaacaacc ttagttctgc
     3001 tttttcttca aataggcggt cacacccagg cccagcaggg cggcgatgga gataacgacg
     3061 atcgggatga tcggcatatc atcgctggtc tgcgggatgg tgctggcctt ggcagcttgg
     3121 gctgcaccgg tggtctgcgg cgtgtacacg cccttgacgt tggcatagcc ggaaagctgc
     3181 ttcgcgcttt ccaaatggcc gcaggtatcg cacttgtagc cccccattgc attctgtcaa
     3241 tgcacagttg atacatttgt gtaaattgtg tggaattttt cccatgcgca caaaaatccc
     3301 gggctggttt gtgcaagttg atggatttgg gctcccatgt aggggccggg catgcccggc
     3361 cctcgcgtga aaggcaggcg gtcgtctgcc taaaagtcgc gggctgcaca tgtgcggccc
     3421 ctacattctc tgccccgggc aagctattga caaccccacc ataaaatgcg acaataaaaa
     3481 caaaattaca gcaggccctg ccaggctttt ggttgaaaat tgcaaaagcc tggcacacaa
     3541 cgctatggaa agccgaggca aaaggcggta tggttaatac aacagaatca acacaccgca
     3601 tacaccgtta caggagggta cttatggcaa agaacgaatt ggacattacg accctgaccc
     3661 cggagcagcg cgacgcgcgc ctggccctgg atgtggagcg cctgctgcgc tttggccgca
     3721 agcacaagct catcaaagac ctggacgttt tggtggcacg caacaccctg ttggacctgc
     3781 tggccctggc cgcccccagc gaggccaagc ccccgaagga ggaccccgcc acccccgccg
     3841 ccctgctgga cgagatggtc gagctggccg cccagaagga actgttcgac ggcgcggtga
     3901 accagtaccg catcaacttt gagacccgcc tgatgggtgc cttgatgccc cgcgagagcg
     3961 aagtctgcaa aaagttcaag aagctctacc agaagcaggg cgcaaaggcc gccaccgact
     4021 ggttctatca gctctgcgtg gacactaact acatccgcac cgcgcagatt gccaagaaca
     4081 tccagtggaa caccgccacc ccctacggcg agctggaaat caccatcaac ctgaccaaac
     4141 ctgaaaaaga ccccaagacc atcgcgctgg agcgtctgca gcccaagagc ggctacccgg
     4201 cctgtatgct ctgcaaggag aatatcggct atgccgggcg catcaacttc cccgcgcggc
     4261 agacccaccg cattgtgccc attacgctgg cgggcgagca gttctacctg caatacagcc
     4321 cctatgccta cttccacgag cattgcatca tgctccacga gcagcacaag cccatggaga
     4381 tgaataaaca gacgctggcc gaaatttttg actttgtggc gcag
//
LOCUS       DBMDSQ010000163         4403 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_4691_length_4403_cov_1.591353, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000163 DBMDSQ010000000
VERSION     DBMDSQ010000163.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 4403)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4403)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4403
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4691_length_4403_cov_1.591353"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..382)
                     /locus_tag="ACZPAX_06685"
     CDS             complement(<1..382)
                     /locus_tag="ACZPAX_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HIR1113621.1"
                     /translation="MQYSIENDILRLTVDTHGAEPVSVIHKPTGAELLWRGDPAVWGR
                     HAPILFPYTGKLTGGKMIAKGVEYAGGQHGFARDVEHTMTHHTEALLEFELHANAETL
                     PKWPYAFVLRSTFTLEGETVHHTLT"
     gene            complement(517..1383)
                     /locus_tag="ACZPAX_06690"
     CDS             complement(517..1383)
                     /locus_tag="ACZPAX_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese transporter"
                     /protein_id="HIR1113622.1"
                     /translation="MELFVDALLDALVDGVKMLPFLYLAYLLIEWLERHHGESIEGAL
                     AGGGRWGFIPGALLGCVPQCGFSAVASNLYASRVITPGTLLAVFIATSDEAIPLLAAE
                     PSQWVTLVLLLACKVAFAIVGGWLLDIPLRHILPHSLYGGYEGHADDVDCHEEHEETS
                     SIFLAALRHTLEIFVFILLFSFIISLLFEGFGEEPITAALSGMGIFQPMLTALVGLVP
                     NCAVSVLLAQLYVQGAISFGSLFAGLTAGAGVGLAVLWRVNPSWKQNLFMTGLLWAIG
                     AAVGMLLQILPI"
     gene            complement(1412..1807)
                     /locus_tag="ACZPAX_06695"
     CDS             complement(1412..1807)
                     /locus_tag="ACZPAX_06695"
                     /inference="COORDINATES: protein motif:HMM:NF017416.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HIR1113623.1"
                     /translation="MYRAREKQMSIYDYLPPYHGELCNANRWVQLAAAIDWDGFEKAY
                     SELFAPGGKVALPARVALGCRIIQLHYGVSDREVVALVQESPYLQYFLGFENFTDEVP
                     FSSRTVARFRARIPDKAVRPAVRLLRSFQ"
     gene            complement(1832..>4403)
                     /locus_tag="ACZPAX_06700"
     CDS             complement(1832..>4403)
                     /locus_tag="ACZPAX_06700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113624.1"
                     /translation="IYRKKRNTVEFTPGQKIQTTPPPQPMPQHVGEPVGEPIETPRNQ
                     PAQPPVSTGFTMQMDALDAKPVDSTQEFMSAYNAVRRSRPAAQPAPAPRPAPAPQPKQ
                     PEPEEDAYDDTLVIGDQSREAIEETGERLLPHRQRDDVDTLVDTLTGRIKLTPVAPAP
                     QPAPAQPAHTGFTQNLGTVQPVPSRPAQAHSGYTQDLAGPAPAEPNTSAFVSDIANAI
                     NSEKEDAAQYAAAAARLTASLAEDAGATRTRARIPTAADVKKAAARLTGPAADEGDDN
                     TVSPFDTAEIPVPHSREYERAEDAPAVRAELAKRLTRATIGCIAAGVSAAVMLVLAVL
                     PARPAALNDATVYPAVMLVLLLAACAANWQTFLSGFKGLAKMPTPDSLAILPALGAAI
                     QCIVLLATGGYTGTLPMMTGPAALVLCLNAVGRRLNAVTVSENFQLVSAKVEHSVAYR
                     LKDAGALKAVSQGLAEPHPSVLVSRPTQIFRSFLTNSAAHGTSDKNQQQFAWVLAACG
                     AAAFLFTVISTKNAATAATAMAGVFCLAAPLAGTLLSALPARMMQRSAAQVGAVIPGW
                     RDIRQLGRINVIQVTARDLFPAGCVTLAGIKPVKPEHIDTAIVYAASILSEAGPTLRD
                     VFMNMLGENRSLLAKVDDRETVYGKGYVGWVNKRRVLVGNRALMQDYGIKIPSLEYEQ
                     HHTVNQRRVIYLAVSGKLFAMFQVAYQRDPDTAAVLETLHHNGLSLVVDCDDFNCDVK
                     LLETAYSLPAGSVKVLSSDEHQAMAPAVAWLPESEGNMLHLGSFASFVGGLEAASGAA
                     EGEHKSAIVLTVSVLLSCVVGVLLTLTGGLVTLPLPGIVLYQAAWCVLALIFPLLQRY
                     "
BASE COUNT          793 a         1365 c         1486 g          759 t
ORIGIN      
        1 cggtcagggt gtggtggact gtctcccctt ccagcgtgaa ggtggagcgc agcacaaagg
       61 cgtagggcca tttggggagc gtttcagcgt tggcgtgcag ctcaaattcc agcagggctt
      121 cggtgtggtg ggtcatcgtg tgctccacat cccgggcaaa gccgtgctgg ccgcccgcgt
      181 actccacgcc cttagcaatc atcttgccgc cggtcagctt gccggtgtag gggaacagaa
      241 tgggcgcatg gcggccccag acagcggggt cgcccctcca gagcaattca gccccggtgg
      301 gcttgtggat gacgctgaca ggctccgcgc cgtgggtatc cacggtcagg cgaaggatgt
      361 cgttttcaat gctgtattgc atagggaagc ctccttataa atatacaaat tgtatagtat
      421 acagttttaa agtgcgttct cacgcgaatg tagggcgggg tgtcctcacc ccgccgcggc
      481 ggcatgaggg catgccgccc tacgcttggt gttattttat atcggcagga tctgcagcag
      541 catacccaca gctgcgccga tggcccagag caggccggtc atgaaaaggt tctgcttcca
      601 gctggggttc acccgccaca gcacggccag gcccacgcct gcaccggcgg tcaggcccgc
      661 aaacagactg ccgaagctga tggccccctg cacgtagagc tgggccagca gcacgctgac
      721 cgcgcagttg ggcacaaggc cgaccagcgc cgtcagcatc ggctggaaga tgcccatgcc
      781 ggacagtgcc gccgtgatgg gttcctcacc aaagccctca aacagcagac tgatgataaa
      841 gctgaacagc aggatgaaca caaaaatttc cagcgtgtgg cgcagagccg ccaaaaagat
      901 gctgctcgtc tcctcgtgtt cctcgtggca gtccacgtcg tcggcgtggc cctcataccc
      961 gccgtagagc gagtggggca ggatatggcg cagcgggata tccagcagcc agccgcccac
     1021 gatggcaaag gccaccttgc aggccagcag cagcaccagc gtcacccact gggacggctc
     1081 agccgccagc agggggatgg cttcgtcact ggtggcaatg aacacagcca gcagcgtgcc
     1141 cggggtgatg acccggcttg cgtacaggtt ggaggctacc gcgctgaagc cgcactgcgg
     1201 cacacagccc agcagcgcac cggggatgaa cccccagcgc ccgccgcccg cgagggcacc
     1261 ttcaatgctc tcgccgtggt ggcgttccag ccattcgatc agcaggtagg ccaagtagag
     1321 aaacggcagc atttttacgc catccaccaa ggcgtccaga agggcgtcta caaacaattc
     1381 catgaatacc tctctataat gaaactatgg gtcactgaaa gctgcgcagc aggcggactg
     1441 ccgggcgcac agccttatcg gggatgcggg cgcggaaccg cgccaccgtg cgggaagaaa
     1501 acggcacctc gtcggtgaaa ttttcaaacc ctaaaaaata ttgcagatag gggctttcct
     1561 gcaccagcgc cacgacctcg cggtccgata caccatagtg cagctggatg atacggcagc
     1621 ccagcgccac gcgggcgggc agggccactt tgccgccggg ggcaaacagt tcgctgtacg
     1681 ccttttcaaa gccatcccag tcgatggcag cggccagctg cacccaacgg ttggcgttgc
     1741 acagctcccc atggtagggc ggcagatagt cgtaaatcga catctgtttt tcgcgcgcac
     1801 ggtacattgg gatttcccct ttctttttac attagtagcg ctgcagcagc gggaagatca
     1861 gcgccagcac acaccacgcc gcctgataca gcacaatgcc gggcagcggc agcgtgacaa
     1921 ggccgccggt cagggtcagc aacacgccga ccacacagct gagcagcaca ctcaccgtca
     1981 gcacgatggc cgacttatgc tcgccctcgg ctgcgccgga ggcggcctcc aacccgccga
     2041 caaagctggc gaagctgccc agatggagca tattgccctc gctctcgggc agccaggcaa
     2101 ccgccggggc catggcctgg tgttcgtcgc tggagagcac cttgaccgaa ccggcgggca
     2161 ggctgtacgc ggtctccagc agcttgacgt cgcagttgaa atcatcgcag tccaccacca
     2221 gggacaggcc gttgtggtgc agcgtttcca gcacggcggc ggtgtcgggg tcgcgctggt
     2281 aggctacctg gaacatggca aacagcttgc cggatacagc caggtagatg acccggcgct
     2341 ggttcacagt atggtgctgc tcatattcca ggctggggat tttgatgccg tagtcctgca
     2401 tcagggcgcg gttgcccacc aggacgcggc gcttgttgac ccagcccaca tagcccttgc
     2461 cgtagaccgt ctcacggtcg tcgaccttgg ctagcaggct gcggttctcc cccagcatat
     2521 tcataaagac gtcccgcagg gtggggccgg cctcggacaa gatggaggcc gcgtagacga
     2581 tggcggtgtc gatatgctcg ggcttgacag gctttatgcc cgccagcgtg acgcaccctg
     2641 ccgggaaaag gtcacgcgcc gtgacctgga taacattgat gcggcccagc tggcggatgt
     2701 cgcgccagcc ggggatgacc gcgcccacct gggcagcgct gcgctgcatc atgcgggcag
     2761 gcagagccga gagcagcgta ccggccaacg gggccgccag gcaaaacaca cccgccatgg
     2821 ccgtggccgc cgtggccgcg ttcttggtgg aaataaccgt gaaaaggaac gccgccgcgc
     2881 cgcaggccgc cagcacccag gcgaactgct gctggttctt atcactggtg ccgtgggcag
     2941 cactgttggt caaaaagctg cggaaaattt gggtcgggcg gctgaccagc acactggggt
     3001 gcggctcggc caggccctgg gagacagctt tcagcgcccc ggcgtccttg agccggtagg
     3061 cgacggaatg ctcgactttg gcgctgacga gctggaagtt ctcgctgacc gtgaccgcgt
     3121 tcaggcggcg gcccacggcg ttcaggcaga gcaccagcgc tgccgggccg gtcatcatcg
     3181 gcagcgtgcc ggtgtagccg ccggtggcca gcagcacaat acactggatg gccgcgccga
     3241 gggcgggcag aatggccagg ctgtccggcg tgggcatctt ggcaaggccc ttgaagccgc
     3301 tcaaaaacgt ctgccagttg gcggcgcagg ctgccagcag cagcacgagc atgaccgccg
     3361 ggtagacggt ggcatcgttc agggcggcag gccgggcagg cagcactgcc agcacgagca
     3421 tgaccgctgc ggacacacct gccgcgatac agccgatcgt ggcccgggtc aggcgttttg
     3481 ccagctcggc acgcacggcg ggggcatcct cggcccgctc atactcacgg ctgtggggca
     3541 caggaatctc cgctgtgtca aacggcgaga cggtgttgtc gtcaccctcg tcggcggcgg
     3601 ggcctgtcag gcgggcggca gcctttttca cgtcggctgc cgtggggatg cgggcgcggg
     3661 tgcgggtggc accggcgtcc tcagccagcg aggccgtcag ccgggccgct gcggcagcgt
     3721 actgggccgc atcctctttt tcgctgttga tggcatttgc aatgtcgctg acgaaagccg
     3781 aggtgttcgg ctctgccggg gcggggccag ccaggtcttg cgtgtagccg ctgtgggcct
     3841 gcgcgggccg tgacgggaca ggctgcaccg tgccgaggtt ctgggtgaac ccggtgtggg
     3901 caggctgtgc aggagcgggc tgcggggccg gggcaaccgg ggtcagcttg atgcgcccgg
     3961 tcagcgtatc gacaagggta tccacatcgt cgcgctggcg gtggggcagc agccgttcgc
     4021 cggtctcctc gatggcctct cgggactggt cgccgatgac gagggtgtcg tcgtaggcgt
     4081 cctcttccgg ttccggctgc ttcggctgcg gggccggggc aggccggggc gctggggcag
     4141 gctgcgcagc cgggcgggaa cggcgcacgg cgttgtaagc gctcatgaat tcctgggtgc
     4201 tgtccaccgg cttggcgtcc agcgcgtcca tctgcatcgt gaagccggtg gagaccggcg
     4261 gctgggcagg ctgattgcgg ggcgtctcga tgggttcgcc caccggctcg ccgacatgct
     4321 gcggcatcgg ctggggcggc ggggtcgtct gtatcttctg gccgggggta aactcgaccg
     4381 tgttgcgctt tttgcggtag atc
//
LOCUS       DBMDSQ010000164         4358 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_4731_length_4358_cov_1.691567, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000164 DBMDSQ010000000
VERSION     DBMDSQ010000164.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 4358)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4358)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4358
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4731_length_4358_cov_1.691567"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..968)
                     /gene="radA"
                     /locus_tag="ACZPAX_06705"
     CDS             complement(<1..968)
                     /gene="radA"
                     /locus_tag="ACZPAX_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015564132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HIR1113625.1"
                     /translation="MPKDKLKTIYVCTQCGETSPRWLGRCPSCGAWNTMTEDVVAEPA
                     KASSGKAAAAPRVTGQTSLVAQKLKNISTTEEKSRIVTGISELDRVLGGGIVIGSVTL
                     IGGEPGIGKSTILLQLCGEVSKTRNVLYVTGEESVRQIKLRAVRLNVPEDNISLVAES
                     DVDEICGLIESMKPDLVVIDSIQTMRCTDISSSSGTVSQVKESSARFLSVAKTLEIPT
                     FIVGHVNKDGAIAGPKVMEHIVDTVLYFEGDKTLPYRVLRAVKNRYGSTNEIGMFDMT
                     GRGLAQIENPSQVMLEGRPLGISGTCVACVMEGTRPVLSEIQALAT"
     gene            1149..1883
                     /locus_tag="ACZPAX_06710"
     CDS             1149..1883
                     /locus_tag="ACZPAX_06710"
                     /inference="COORDINATES: protein motif:HMM:NF027796.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5058 family protein"
                     /protein_id="HIR1113626.1"
                     /translation="MDFNVNAPILFVLVGIIVAFVLAQSAFFLWRAVNRAREIGLPQE
                     TIRKTISAAAVFTVAPAVAILVGVISLSKSLGIALPWLRLSVVGSLTYETVAAGTSLT
                     ELGLDTNTPIPTASDYVTVAAVMTVGIMVGLVLVPLLTKRIQGGMMKLGAKDHRWADI
                     FNNAMFLGMISAFLGYVFSDVMNVTHGDFSGLIPVLVMLTSAVVMVICGVAAMRTKKR
                     WIQDYALPISMLCGMAVSIPLTSWLS"
     gene            1898..2578
                     /locus_tag="ACZPAX_06715"
     CDS             1898..2578
                     /locus_tag="ACZPAX_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009002797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113627.1"
                     /translation="MEKTVLEQKETYVDSVHRWGRIWGLTLLVLIVAYPIAVSLIFHA
                     QINWPAFIKGFIAIGPMYWAVGIVEIFTYVPMLGAGGSYLSFVTGNISNLKLPCALDA
                     MERAGVKSNSEEGEVISTIAIAVSSIVTTLIILVGVVLIIPLTPLLSSPILAPAFSQI
                     LPALFGGLAVVFVSKNFRLALAPLLLMLVLFIFVPALNASTVGIMVPVGVIFSLVVAR
                     ILYKKGLV"
     gene            2600..4036
                     /locus_tag="ACZPAX_06720"
     CDS             2600..4036
                     /locus_tag="ACZPAX_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009002796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HIR1113628.1"
                     /translation="MIVLWLILAVVVVLAAVVLVRTARFTPGTAPAVPEGEETIDRDK
                     AVENLRTLIRCRTVSNTNHALEDDAEFEKLINTLPTLYPNVFKTCKVERLEDRGLLFT
                     WSGKEHTEPTVLMAHYDVVDIDEAGWKKPPFEGVLEDGVLWGRGALDTKVTFNGVLTA
                     ADKLIGEGYTPAQDIYFAFSGGEEVNGLGAVHIVDWFEANHITPALVVDEGGAVVEDV
                     FPGVKQPCGLIGIAEKGMLNVEYKVKSGGGHASAPKPHSPLVALSEACTKIESHPFKA
                     HVTKPVAEMFDTLGRYSNVTYRMIFANLWLFGGVLDSICRKSGGELNALMRTTVAFTQ
                     AKGSAGRNVIPPEATLISNLRLNPEDTMDSALAYLKETINDPAVEITKLEGMNPSRIS
                     ETNCPAWDKVASAVAGTWKGCLVSPYLMVQCSDSRHYGRISDHVYRFSAMDLTAEERG
                     TLHGNNERIRVETVGRAVEFYVRLMRQC"
     gene            complement(4169..>4358)
                     /locus_tag="ACZPAX_06725"
     CDS             complement(4169..>4358)
                     /locus_tag="ACZPAX_06725"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016321566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR1113629.1"
                     /translation="TRVNENIDDETFGMLFIADINSFEELHSEIEKILISDKLVENWT
                     YPLIQPILIEEAKKRGFI"
BASE COUNT          880 a         1240 c         1235 g         1003 t
ORIGIN      
        1 ttggtggcaa gggcctgtat ttcgctcagc accgggcggg tgccctccat cacgcaggcc
       61 acgcaggtgc cgctgatgcc caagggccgc ccttccagca tgacctggga gggattctcg
      121 atttgcgcca gccctcggcc ggtcatatcg aacataccga tttcgttcgt ggagccgtaa
      181 cggttcttga cggcccgcag cacgcggtat ggcagggtct tgtcaccctc aaagtaaagc
      241 accgtatcaa cgatgtgctc catgaccttg gggcctgcaa tcgcaccgtc cttgttgaca
      301 tggccgacga tgaaggtggg gatttccagc gttttcgcta cacttaaaaa gcgggcggaa
      361 ctctccttca cctggctgac cgtgccggac gacgacgaaa tatcggtgca gcgcatcgtc
      421 tggatggagt cgatgacgac caagtcgggc ttcatcgatt cgatcaggcc gcatatctca
      481 tccacgtcgc tctcggcgac cagcgagata ttatcctcgg gcacattcag gcgcacagca
      541 cgcagtttga tctgccgcac cgattcctcg ccggtgacat agagcacatt gcgggtcttg
      601 ctgacctcgc cgcagagctg cagaagaatc gttgacttgc cgatgcccgg ctcgccgccg
      661 atgagcgtca cactgccgat gacgatgcca ccacccagaa cgcgatccag ctcgctgatg
      721 ccggtgacga tgcggctttt ttcctcggtc gtgctgatgt tttttaattt ctgtgccacc
      781 aggcttgtct gcccggtcac gcgcggcgcg gcagcggcct tgccgctgga tgccttggcg
      841 ggttctgcca ccacatcctc ggtcatagtg ttccacgccc cgcaggaggg gcagcgcccc
      901 agccagcggg ggctggtctc gccgcattgg gtgcagacat agatggtttt gagtttatct
      961 ttgggcatgg acgcctccct gaactatact ttacctatta tagcccaaaa tgggcgcggg
     1021 ggcaagtatc cactgaacaa aaacatttgt agctgtaaaa tgtacttttt ctcttgctat
     1081 tttttgaaca atgtgttaaa gttaaaaaac gctttaaagc tataaagcaa aagaaaaggt
     1141 gtggtgttat ggacttcaac gtgaacgcgc cgatcctgtt tgtgctggtc ggcatcattg
     1201 tggcgttcgt gctggcacag tcggcgtttt ttctgtggcg tgcggtcaac cgcgcaaggg
     1261 aaatcggtct gccgcaggaa accatacgca agaccatttc ggcggcggcc gtctttacgg
     1321 tagcaccagc agtggccatt ctggtgggcg ttatctccct gagcaagagc ctgggtatcg
     1381 ccctgccgtg gctgcgccta tcggtcgtag gatccctgac ctatgagacc gtcgcggcgg
     1441 ggacctcact gacggagctg gggctggaca ccaatacccc catccctacg gcgtcggact
     1501 atgtcacggt ggccgccgtt atgacggtgg gcatcatggt cggcctggtc ttggtgccgc
     1561 tgctgaccaa gcgtatccag ggtggtatga tgaaactggg cgcaaaggat caccgctggg
     1621 cagatatttt taataatgcc atgttcctcg gtatgatcag cgccttcctc ggctatgtgt
     1681 tcagcgacgt gatgaatgtg acccacggcg atttctcggg tcttatcccg gtgctcgtca
     1741 tgctgacgtc ggcggtcgtg atggttattt gcggcgtggc ggccatgcgc acgaaaaagc
     1801 gctggataca ggactatgcg ctgccaatca gtatgctgtg cggcatggca gtgtccatcc
     1861 cgctgacaag ctggctgagc tgaaaggagc ggtactcatg gaaaagacag ttctggaaca
     1921 gaaagaaacc tatgtcgaca gtgtgcaccg ctggggccgc atttggggcc tgacgctttt
     1981 ggtgctgatt gtggcatacc cgatcgcagt gtcgctcatc ttccacgcac agatcaactg
     2041 gcccgcgttc attaagggct ttatcgccat cgggcctatg tactgggccg tcggcattgt
     2101 ggaaattttt acctatgtgc cgatgctcgg cgcgggcggc agctacctgt ccttcgtcac
     2161 cggtaacatc tccaacctga aactcccctg cgcccttgac gccatggagc gggcgggcgt
     2221 caagtccaac agtgaggaag gtgaggtcat ttctaccatc gccattgccg tgtcaagcat
     2281 cgtgacgacg ctgattattt tagtgggcgt ggtgctcatc atcccgctga caccgctgct
     2341 ctcctccccc atcctagccc cggcgttctc gcagatttta cccgcactgt tcggcggtct
     2401 ggcggtggtg tttgtctcca aaaacttccg cctggcgctg gccccactgc tgctgatgct
     2461 ggtgctgttc atctttgtac ccgcgctcaa tgcgtccacg gtaggtatca tggtgccggt
     2521 cggtgtcatt ttcagccttg tagtggcgcg tatcctgtac aaaaagggcc ttgtctaagc
     2581 ccataaggag gaatacaaaa tgatcgtttt atggttgatt ctggcggttg tcgtcgtgct
     2641 ggccgccgtg gtgctggtgc gcacggcccg ctttacgccg ggcactgccc ccgctgtgcc
     2701 cgagggcgag gaaaccatcg accgtgacaa ggccgtggaa aatctgcgca ccctcatccg
     2761 ctgccgtacc gtctccaaca cgaaccacgc gctggaagac gatgccgagt ttgaaaagct
     2821 catcaatacc ctgccgacgc tctaccccaa cgttttcaag acctgcaagg ttgagcgcct
     2881 tgaggaccgt ggcctactgt tcacctggtc cggcaaggaa cacaccgagc cgaccgtatt
     2941 gatggctcac tatgacgtcg tcgacattga cgaggcaggc tggaagaagc cgccgtttga
     3001 gggcgtgctg gaagatggcg tgctctgggg ccgcggtgcg ctggacacga aagttacttt
     3061 taacggcgtg ttgaccgctg ctgacaaact gattggcgag ggctacaccc ccgcgcagga
     3121 tatctacttt gcattcagcg gcggcgagga agtcaatggc ctgggcgcgg tacacattgt
     3181 agattggttt gaagccaacc acattacccc ggcgctggtt gtggatgagg gcggcgcggt
     3241 cgtcgaggat gtgttccccg gcgtcaagca gccctgcggc ctgatcggca ttgcggaaaa
     3301 aggtatgctg aacgtggagt acaaggtcaa gtccggcggc ggccacgcca gcgcgcccaa
     3361 gccccacagc ccgcttgtgg ccctgagcga agcctgcacc aagatcgaga gccacccctt
     3421 caaggcgcac gtcacaaagc cagtggccga aatgttcgat acgctgggcc gttactccaa
     3481 cgttacctat cgcatgatct ttgccaacct ctggctgttc ggcggcgtgc tggattccat
     3541 ctgccgtaag agcggcggcg agctgaacgc cctgatgcgc acgacggtgg cctttaccca
     3601 ggccaagggc agtgcgggcc gcaacgttat cccgcccgag gcaacgctca tcagcaacct
     3661 gcgcctgaac cccgaggaca cgatggacag tgctcttgcc tacctgaaag agaccatcaa
     3721 cgaccccgcc gtcgagatca cgaagctgga aggcatgaac ccgagccgta tcagcgagac
     3781 gaactgccct gcctgggaca aggtcgccag cgctgtggca ggcacctgga agggctgtct
     3841 tgtctcgccc tatttgatgg tgcagtgctc cgacagccgt cactatggcc gcatcagcga
     3901 ccatgtgtac cgcttctccg ccatggatct gaccgccgag gagcgcggca ccctgcatgg
     3961 caacaacgaa cgcatccgcg tcgagaccgt cggtcgtgcc gtggagtttt acgtccgttt
     4021 gatgcggcag tgctgagaaa ataagcatac aaagaggtgc agcccgtttg aagtgctgca
     4081 ccttttactg tttatatccc gaaatgtata gagggcggca ttctcgatgc ccaatcatac
     4141 aaactgaaag tttccgttgc ttgtctattt atataaagcc tctttttttt gcttcttcaa
     4201 tcaatatagg ttgaattaaa ggatatgtcc aattttcaac taacttatct gaaatcaata
     4261 tcttctcaat ctcactatgt aattcctcaa atgagttaat gtctgcaata aataacattc
     4321 cgaaagtttc atcatcaata ttttcattta ctcgtgtc
//
LOCUS       DBMDSQ010000165         4307 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_4793_length_4307_cov_2.338088, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000165 DBMDSQ010000000
VERSION     DBMDSQ010000165.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 4307)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4307)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4307
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4793_length_4307_cov_2.338088"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(146..892)
                     /locus_tag="ACZPAX_06730"
     CDS             complement(146..892)
                     /locus_tag="ACZPAX_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HIR1113630.1"
                     /translation="MGVNKQNVKIHVEHLKKNFGNLEVLRDVSTDIYEGEVVVILGPS
                     GSGKSTFLRCLNRLEEITGGTVIVDGHDLTDKKTDLNKVRENVGMVFQHFNLFNNLNV
                     MGNMTLAPVELKKATKGEAKEKALHLLNRVGLSDKAEAYPAQLSGGQKQRVAIARALA
                     MDPDIMLFDEPTSALDPEMVGEVLQVMKQLAAEGMTMVVVTHEIGFAREVASRVIFME
                     GGYIVEEGTPDEVINHPKQKRTIDFLSKVL"
     gene            complement(879..1574)
                     /locus_tag="ACZPAX_06735"
     CDS             complement(879..1574)
                     /locus_tag="ACZPAX_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014354665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HIR1113631.1"
                     /translation="MAKILANYGGLLAAAMGQTLLLALCGLFFACIIGLVVGLMSVVK
                     NRACNIIATIFVDVIRGVPMIVLAFYVFFAVPMLLNNIMHLNTMLGINTQLTLTALQA
                     GTICLALNCGAYMAEIIRAGIESVDKGQMEAARSLGLSYWKAMAKVVLPQAIRTMIPS
                     IINQFIITLKDTSILSVIGFPELVNTAKNVVANTMSAFQVWSIVALMYLIVITLLSRL
                     AKRVERRLNRGRQ"
     gene            complement(1824..2639)
                     /locus_tag="ACZPAX_06740"
     CDS             complement(1824..2639)
                     /locus_tag="ACZPAX_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007048001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HIR1113632.1"
                     /translation="MKKLLAFGVASVMALSLAACGGASSTAASSEAADTASSGAATTE
                     ATGKKWTIATDTVFKPFEYTDASGNFVGIDVDILAAIAEDQGFEYELKSLGWDASIAA
                     CQAGQADGMIAGASITDERKASGWTFSDGYYDSTQTLTVGADSTIAGFADLSGQTIAV
                     KTGTQGASYAESLKDEYGFNITYFEDSPTMYQAVLGGQCVGCFEDTPIMKASIKDGDL
                     ALKVLDDTASDPAPYGFAIFNADNQELLDAFNAGLADIKANGKYDEILAKYLG"
     gene            complement(2905..>4307)
                     /locus_tag="ACZPAX_06745"
     CDS             complement(2905..>4307)
                     /locus_tag="ACZPAX_06745"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015574303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1113633.1"
                     /translation="MFIQIAGTLFLLFVLNWRLSLLVVVGYIAMFWYINYSTRRSRAY
                     YASQQASLGELDAYMEEMIAGQKVVKVFNHQQADMAAFAARNEKLRQAGTGAQSYAAT
                     MIPAVVSIGYINYAIIAVVGGLMVMWGWSDLGSLASYLVFVRQTTLPINQFTQQGNFL
                     LAALAGAERIFTVMEERPEIDEGTVELVRVRENADGTLTECAEKTGRWAWCDRSRPDV
                     PPEPLTGDVRFHDVSFGYTPERMILQNLSLYAKPGQKIAFVGSTGAGKTTITNLINRF
                     YEVSGGSITYDGMDVRLIKKNDLRRSLGVVLQDTHLFTGTVADNIRFGKLDATQEEIE
                     RAARIANADSFIRRLPRGYDTMVTADGANLSQGQRQLLAIARAAVADPPVLILDEATS
                     SIDTRTEALIQKGMDGLMEGRTVFVIAHRLSTVRNADAIIVLEHGHIIERGTHEELLA
                     KQGEYYQLYHGMFELS"
BASE COUNT          840 a         1278 c         1298 g          891 t
ORIGIN      
        1 atgacggatg tctggagact gcctgctatt ggacgttggg cgcgtttgcc gttaccaccc
       61 attcacgggc agcagcggca aacgcgcccc tcatccggcc tgcggccacc ttccccccaa
      121 ggggaaggct ttatctgttt tccgcttaca gcaccttact caagaaatcg atcgtccgct
      181 tctgtttcgg gtggttgatg acctcgtcgg gggtgccttc ctcaacgata tacccaccct
      241 ccatgaagat cacacggctg gcgacctcgc gggcaaagcc gatctcgtgg gtgacaacga
      301 ccatcgtcat accctcggcg gcaagctgct tcatgacttg caggacttca ccgaccattt
      361 ccgggtccag cgcactggtc ggctcgtcaa acagcatgat atccgggtcc atggccaggg
      421 cgcgggcaat agccacgcgc tgcttctggc cgccggacag ctgcgccggg taggcttcgg
      481 ccttgtcgga cagacccacg cggttcagca ggtgcagggc cttctccttg gcttcaccct
      541 tcgtggcctt tttcagctcc accggggcca acgtcatgtt gcccatgacg ttgaggttat
      601 tgaacaggtt gaagtgctgg aacaccatgc cgacattctc gcgcactttg ttcaaatcgg
      661 ttttcttgtc ggtcaggtcg tggccatcca caatgactgt gccgccggtg atttcttcca
      721 ggcggttcag gcagcgcaga aaagtcgact tgccgctgcc ggaggggcct aggatgacaa
      781 cgacctcgcc ctcatagata tccgtcgata catcgcgcag gacctcgagg ttgccgaagt
      841 tttttttcaa atgctcaacg tgaattttga cattctgctt attgacgccc acggttgagc
      901 ctcctctcca cccgcttggc aaggcgggac agcagtgtaa tgacgatcag gtacatcagc
      961 gcgacaatgc tccacacctg gaacgcgctc atcgtgttgg ccacgacgtt cttggcggtg
     1021 ttgaccagct cggggaagcc gatgaccgac aggatggacg tgtctttcag cgtgatgatg
     1081 aactggttga tgatggaggg gatcatcgtg cggatggcct ggggcagcac gaccttggcc
     1141 atggccttcc agtacgacag gcccaggctg cgggcggcct ccatctggcc cttgtccacg
     1201 ctctcgatac cggcgcggat aatttccgcc atgtatgcac cgcagttcag cgccaggcag
     1261 atggtgcctg cctgcagggc ggtcagcgtg agctgcgtgt tgatgcctag catggtattc
     1321 aggtgcatga tgttgttgag cagcatcggc actgcgaaga acacgtagaa cgcaagcacg
     1381 atcataggca cgccgcggat gacgtcaaca aaaatcgtag cgatgatgtt gcaggctcgg
     1441 tttttgacga cactcataag gccgacgacc aggccgatga tgcaggcaaa gaacagcccg
     1501 cacagcgcca gcagcagcgt ctggcccata gcggccgcca gcagcccgcc gtagtttgca
     1561 agaattttag ccaagggtca agactccttt cggggtggtt ggatacaagg cttcccccct
     1621 cgggggaagg tggccgcaaa gcggcctgat gaggggcagc ctgcccgcag ctgcccctgt
     1681 atgaggcggc ccggcagact cggccctcac ccggcgctgc ggcgccaccc tccccctcgc
     1741 gggggagggc aagggatttt cgcagaggcc acaaaagcgg ggccgcccgc aaaaacaggc
     1801 gtccccgcgt gctttattgt ttgttagccc aggtactttg ccaggatctc atcatacttg
     1861 ccgttggctt tgatgtcagc cagaccggcg ttgaaggcat ccagcagctc ctggttgtca
     1921 gcgttgaaga tggcgaagcc gtagggggcg gggtcgctag cggtgtcatc caaaaccttc
     1981 agggccaggt cgccgtcctt gatggaggcc ttcatgatgg gggtatcctc aaagcagcca
     2041 acgcactggc cgcccaggac agcctggtac atcgtggggg aatcctcaaa gtaggtgatg
     2101 ttgaagccgt actcgtcctt caggctctcg gcataagaag caccctgggt gccggtcttg
     2161 acggcaatgg tctggccgct caggtcggcg aagccggcga tggtggaatc cgcgcccacg
     2221 gtcagggtct gggtgctgtc atagtagccg tcggagaagg tccagccgct cgccttgcgc
     2281 tcatcggtga tggaggcgcc ggcgatcatg ccgtcagcct ggccggcctg gcaggccgcg
     2341 atggaagcat cccagcccag gctcttgagc tcatactcaa agccctggtc ctcggcgatg
     2401 gccgccagga tgtctacgtc gatgccgaca aagttgccgg aggcgtcggt gtactcaaag
     2461 ggcttgaaca ccgtgtcggt agcgatggtc cactttttgc cggtggcctc ggtggtggca
     2521 gccccagagg aggcagtatc ggcggcttcc gaggaagcag ccgtggagga tgcgccgccg
     2581 caggcagcga gagacagggc catgacggag gccacgccga atgcaagcag ttttttcatg
     2641 agatactttc ctttccactc cacaaatttt tccgccgccg cgcggcgggc cgatgcagaa
     2701 ccaaaaaagg gcaatagcgc catgggacca tgctgctatc gcccttgata gtttctgctg
     2761 tatcgtactt ataattatag cacaccgtcg gtaaaagtca ataccaaaaa acagttacag
     2821 ctttattaaa ttaagtttcg ccgccgtttc gacgatgttt ttgtaaattt gtgcgtgatg
     2881 cagggacaca aggttgcata tgactcacga cagctcgaac ataccgtggt agagctggta
     2941 gtattcaccc tgctttgcca gcagttcctc atgggtgccg cgctcaatga tatgcccgtg
     3001 ttccagcaca atgatggcgt cggcgttgcg cacggtggac agccggtgcg cgatgacaaa
     3061 caccgtgcgg ccctccatca ggccgtccat gcctttttgg atgagcgcct cggtgcgggt
     3121 gtcgatactc gacgttgctt cgtccaatat cagcaccggc gggtcagcca ccgccgcgcg
     3181 ggcaatggcc agcagctgcc gttgtccctg gctcagattc gcaccgtcag ccgtgaccat
     3241 cgtgtcatac cccctgggca ggcggcggat gaaggagtcc gcgttggcga tcctggccgc
     3301 ccgctcaatt tcctcctgcg tggcatccag cttgccgaaa cggatgttgt ccgccacggt
     3361 gccggtgaac aaatgtgtat cctgcaggac gacacccagc gaacggcgca gatcattttt
     3421 cttgataagc cgcacatcca tcccatcgta ggtgatggag ccgccggaca cctcatagaa
     3481 gcggtttatc aaattggtga tggtcgtttt gcccgcgccg gtggagccaa cgaaggcgat
     3541 tttctggccc ggtttggcgt aaagcgagag gttttgcaaa atcatccgct cgggcgtgta
     3601 gccaaagctg acgtcgtgga accgcacatc gccggtcaac ggctcgggcg gcacatcggg
     3661 gcggctgcgg tcacaccagg cccaacggcc ggtcttttcg gcgcattcgg tcagggtgcc
     3721 gtcggcgttt tcccgcacgc ggaccagctc caccgtgccc tcgtcgatct cggggcgctc
     3781 ttccatgacc gtaaagatac gctccgcgcc cgccagcgcc gccaacaaaa agttgccctg
     3841 ctgggtaaac tggttgatgg gcagggttgt ctggcgcaca aacaccaggt agcttgccag
     3901 gctgcccaaa tcggaccagc cccacataac catcaggccg cccaccaccg caatgatggc
     3961 gtagttgatg taaccgatac tgacgacggc ggggatcatc gtggcggcgt agctctgggc
     4021 accggtgccg gcttggcgca gtttttcgtt gcgggcggca aaggcggcca tgtcggcctg
     4081 ctggtggtta aagaccttga ccaccttctg gcccgcgatc atttcctcca tgtaggcgtc
     4141 cagctcgccc aagctggcct gctggctggc gtagtaggcg cggctgcgcc tggtggagta
     4201 gttgatgtac cagaacatgg caatgtagcc caccacgacc agcagcgaga gccgccagtt
     4261 gagcacgaac agcaaaaaca gcgtgcctgc gatctgtata aacatct
//
LOCUS       DBMDSQ010000166         4295 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_4806_length_4295_cov_1.845732, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000166 DBMDSQ010000000
VERSION     DBMDSQ010000166.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 4295)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4295)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4295
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4806_length_4295_cov_1.845732"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..652
                     /locus_tag="ACZPAX_06750"
     CDS             <1..652
                     /locus_tag="ACZPAX_06750"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015537501.1"
                     /note="catalyzes the dehydration of
                     2,3-dihydroxy-3-methylbutanoate to 3-methyl-2-oxobutanoate
                     in valine and isoleucine biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HIR1113634.1"
                     /translation="VPTVTGKTLGENIQGAKILNNKAIRSIDNPYSQTGGLQILWGNI
                     APDGCVVKRSAVAPEMQVHSGPARVFDSEDTAIAAIYAGDIHPGDVVVIRYEGPKGGP
                     GMREMLNPTSALAGMKLDTTVALITDGRFSGASRGAAIGHVSPEAASGGPIGLVKEGD
                     TIEIDIPNASIHLAVSDDELAARKAAYIQPEPNIKTGWLARYARLVTSANLGAVLK"
     gene            complement(1006..1521)
                     /locus_tag="ACZPAX_06755"
     CDS             complement(1006..1521)
                     /locus_tag="ACZPAX_06755"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HIR1113635.1"
                     /translation="MPFITVNAHSPADYGAAFAAENATIAGSVRLEKNSSVWYGAVLR
                     ADTGRITIGENSNVQDNAVLHTGPGLDVTIGRGVSIGHGAIVHGCTVGDNCLIGMHAT
                     ILNGAVIGAGSLIAAGALVPEKMVVPAGSLVIGVPGKVMHPVSAAQAAGIKANEEEYL
                     ELARAHKNAFP"
     gene            1661..2434
                     /locus_tag="ACZPAX_06760"
     CDS             1661..2434
                     /locus_tag="ACZPAX_06760"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005922152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR1113636.1"
                     /translation="MSIKMICSDVDGTLLQYGRQKLEGEIFDQIRALHDRGILFCPAS
                     GRQYTSLRLLFEPVADCCVFLCENGGVLFKDEQCIAENPMPRALAEEIARDMWQNSDG
                     RGEVMLSGQNCAYLMERGLGMLDRIKFIGNRYQIIHDPAEVPEEIVKTSVYLHEGVEK
                     YVDRFVPRWQQANAAVAGPFWIDTTTANKGIGVQSLCRVLGIAPDEVMAFGDNFNDVS
                     MLDIVDTPYIMDSAAPALRARYPLHTPRPEDVLRDFLAK"
     gene            2629..2889
                     /locus_tag="ACZPAX_06765"
     CDS             2629..2889
                     /locus_tag="ACZPAX_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HIR1113637.1"
                     /translation="MKEFTYTIKEPVGIHARPAGMLAKEAKTCQSTITIVDKNGKSVD
                     ATRLMALMGMGIKCGDTVTVKIEGADEETAAPAMEKFFNEHL"
     gene            2964..>4295
                     /gene="ptsP"
                     /locus_tag="ACZPAX_06770"
     CDS             2964..>4295
                     /gene="ptsP"
                     /locus_tag="ACZPAX_06770"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015528951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HIR1113638.1"
                     /translation="MITIQGKGVSAGVGVGPLYFYRRATAEIKRYTVEDTDAEWHRFK
                     GAQTGAVEQLGVLAEQARKEAGDEAAMLFETHQMMAEDLDYEEAIEDRILNQKMNAEA
                     AVSDTAEQFAEMFASMDDAYMQARAADVKDVSQRILSILCGIVQGGIASDVPVLLCAD
                     DLAPSETIQLDKSKILGFITAGGSGSSHTAILARTMGIPAIVGMGDALKPEYEGRQAI
                     ADGATGALVIDPDDDTRDRLLKKRDEQLRLQRLLETLKGQANVTKDGKTIRIYCNIGS
                     PEDVHAVQVNDGGGIGLFRSEFLYLNTSDYPTEDQQFEAYKQVLSDMDGKEVIIRTLD
                     IGADKQIGYFNLPKEDNPAMGMRALRICLTRPEIFKTQLRALFRASAFGKLGIMFPMV
                     TSVWEVREAKKLCEEVKRELKHEGTPYSEDVQIGIMIETPAAAVMSDRLAKE"
BASE COUNT          889 a         1345 c         1255 g          806 t
ORIGIN      
        1 tgtgcccacc gtcaccggca agaccctggg ggagaacatc cagggcgcga agattctcaa
       61 caacaaggcc atccgctcta tcgacaatcc gtacagccag accggtggtt tgcagatcct
      121 gtggggcaac attgcgccgg acggctgtgt cgtcaagcgc agcgccgttg cgcctgaaat
      181 gcaggtacac agcggcccgg cccgcgtctt tgacagtgag gacaccgcca ttgcggccat
      241 ctacgcaggg gacatccacc ccggcgacgt cgtagtcatc cgctacgaag gccccaaggg
      301 cggtcccggt atgcgtgaga tgctcaaccc caccagtgcg ctggcgggca tgaagctgga
      361 caccaccgtg gcgctcatca ccgatggccg cttctccggt gcgtcccgcg gcgcggccat
      421 cggccatgtc agccccgagg cagcctccgg cggccccatc ggcctcgtca aggagggtga
      481 caccatcgag atcgatattc cgaatgcgtc catccacttg gccgtcagtg acgacgagct
      541 tgccgcccgc aaagccgcct acatccagcc cgagccgaac atcaagaccg gctggctggc
      601 acgctacgca aggcttgtta caagcgccaa cctgggtgcg gtgctgaaat aaaaacaaaa
      661 acggatgctg tggcaaaacc acagcatccg ttttttcttt tagggcaata ccgcataatt
      721 ctaagtgccg atacggcgag cgaggtgcgg cagctgctaa gccaaaagcg cagataatac
      781 tttgtgtatt atcgagcatt ttggcaacgc agatgccgta ccgcagccgc cggagcggtg
      841 cttaagccgt caggcgggaa ttgtgcggtg ttgccttagg cttcccccga ggggggaagc
      901 tgtcaccgaa ggtgactgat gcggggcagc gttttcttta tcgcccatta ccgggttact
      961 gccggaacgc cgcccctcat ccggcgctgc gcgccacctt ccccctcagg ggaaggcatt
     1021 tttgtgtgcc ctcgccaatt ccaaatattc ttcctcgttt gctttgattc ctgcggcctg
     1081 cgccgcactg accgggtgca tgacctttcc cggcacgccg atgacaaggc tgcctgcggg
     1141 cacgaccatc ttttccggca ctagcgcccc tgcggcaatc aaactgcccg cgccgatgac
     1201 tgcgccgttc aaaatcgtgg cgtgcatccc aattaaacag ttatcgccca ccgtgcagcc
     1261 gtggacgatg gctccatgcc cgatggacac gccgcgccct atcgtcacat ccagcccggg
     1321 gccggtgtgc agcacagcgt tatcctgcac gttggagttc tcgccgatgg tgatgcgccc
     1381 ggtatcggcc cgcagcaccg cgccatacca gacgctgctg ttcttttcca gccgcacgct
     1441 gcccgcgatt gtcgcgtttt ccgctgcgaa ggccgcaccg taatcggcag gggagtgggc
     1501 gttcactgtg ataaaaggca taggttttct tcctttcgat atcgtcctac ggggcgtcgg
     1561 ggacgccgcc ccgtatatct attatacaaa atgaaatcga aattgaaaag tcaccgccgc
     1621 ctgtggtaca ataaaagcgc gaaataaaag gagttgtact atgtccatca aaatgatctg
     1681 cagcgacgtt gacggtacgc tgctgcaata tggccgccag aagctggaag gcgaaatttt
     1741 tgaccagatc cgcgccctgc atgaccgggg catcctgttc tgcccggcct ccgggcggca
     1801 gtacaccagc ctgcgcctgc tgtttgagcc ggtggctgac tgctgcgttt tcctgtgcga
     1861 gaacggcggc gtcctgttca aagatgagca gtgcatcgcc gaaaacccaa tgccccgcgc
     1921 cctggcggag gaaattgccc gcgatatgtg gcagaacagc gacggccggg gtgaggtcat
     1981 gctctccggc cagaactgcg cctacttaat ggagcgcggg ctggggatgc tggatcgcat
     2041 caagttcatc ggcaaccgct accaaatcat ccacgacccc gccgaggtgc ccgaggagat
     2101 cgtcaagaca tcggtctacc tgcatgaggg cgtggaaaaa tacgtcgacc gctttgtgcc
     2161 gcgctggcag caggccaacg ctgccgtggc cgggcctttc tggatcgaca cgaccaccgc
     2221 caacaagggc atcggcgtac agtccctttg ccgcgtgctg ggcattgccc ccgatgaggt
     2281 catggccttt ggcgacaact ttaacgatgt atcaatgctg gacatcgtgg acacccccta
     2341 catcatggac agcgccgccc ccgcgctgcg cgcccgttat ccgctgcaca ccccgcgccc
     2401 cgaggacgtg ctgcgggatt ttcttgcaaa atgacgccaa atgacaaaat ctcccatgtt
     2461 atggcttttc ggtgcagtcc ctgccgaaaa tttttaacaa attcaccaaa gattaggggt
     2521 gtttttggct ctttttccgt tgacgtatac ggcgcagtat gttattataa taacagctta
     2581 atgattgcgc cgcgctctac cgctgcgcac ttatgggagg aaaaagctat gaaagaattc
     2641 acgtatacca tcaaagagcc ggtcggcatc catgcacgtc ccgccggtat gcttgcaaaa
     2701 gaggccaaga cctgccagag caccatcacc atcgtggaca agaacggcaa gtccgttgac
     2761 gccacccgcc tgatggctct gatgggcatg ggcatcaagt gcggcgacac cgtcaccgtc
     2821 aagattgagg gcgccgacga ggagaccgcc gcccccgcca tggagaagtt cttcaacgag
     2881 catctgtaat tccgatttca tttacggccg cggccgccac accagaacgg cgggcgcggc
     2941 cctttttgta aggaggtttt cccatgatca cgattcaggg caagggcgtt tccgccggtg
     3001 tcggcgttgg cccgctctac ttctaccgcc gcgccacggc cgagatcaag cgctataccg
     3061 tagaggatac agacgccgag tggcaccgtt ttaagggggc ccaaaccggc gcggtcgagc
     3121 aactgggcgt tctggccgag caggcccgca aggaagccgg cgacgaggcc gccatgctgt
     3181 ttgagaccca ccagatgatg gccgaggacc ttgactacga ggaggccatc gaggatcgca
     3241 tcctcaacca gaagatgaac gccgaggccg ccgtctccga cacagccgag cagtttgccg
     3301 agatgttcgc ctcgatggac gatgcctaca tgcaggcccg cgccgctgat gtcaaggacg
     3361 tcagccagcg catcctcagc atcctgtgcg gcatcgtgca gggcggtatt gcgtccgatg
     3421 tgccggtgct gctctgcgcc gacgacctag ccccctccga gacgatccag ctggataagt
     3481 cgaagattct gggcttcatc acggcgggcg gctccggctc cagccatacg gccattctgg
     3541 cccgcaccat gggcatccct gccatcgttg gcatgggcga cgccctgaag cccgagtacg
     3601 agggccgcca ggccattgcg gacggtgcta ccggcgcact ggtcattgac cccgacgacg
     3661 acacccgcga ccgcctgctg aagaagcgcg acgagcagct gcgtctgcag cgcctgctgg
     3721 aaacgctgaa aggccaggcc aacgtgacga aggacggcaa gaccatccgc atctactgca
     3781 acatcggctc gcccgaggat gtccacgccg tacaggtcaa cgacggcggc ggcattggcc
     3841 tgttccgctc tgagttcctg tacctgaaca ccagcgacta tccgaccgag gaccagcagt
     3901 ttgaggccta caagcaggtg ctctccgaca tggacggcaa ggaagtcatc atccgcacac
     3961 tggacatcgg cgcggacaag cagattggct acttcaacct gcccaaggag gacaaccccg
     4021 ccatgggtat gcgcgcgctg cgcatctgcc tgacccggcc ggagattttc aagacccagc
     4081 tgcgcgcact gttccgtgcg tcggctttcg gcaagctggg cattatgttc ccgatggtca
     4141 cgtccgtctg ggaagtccgc gaggcaaaga agctctgcga ggaagtcaag cgtgaactga
     4201 agcacgaggg cacgccgtac agcgaggacg tgcagatcgg tattatgatc gagacgcccg
     4261 ccgccgctgt gatgagcgac cgcctggcga aggaa
//
LOCUS       DBMDSQ010000167         4289 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_4817_length_4289_cov_1.664995, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000167 DBMDSQ010000000
VERSION     DBMDSQ010000167.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 4289)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4289)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4289
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4817_length_4289_cov_1.664995"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..667)
                     /locus_tag="ACZPAX_06775"
     CDS             complement(<1..667)
                     /locus_tag="ACZPAX_06775"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005930056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:0051991 -
                     UDP-N-acetyl-D-glucosamine:N-acetylmuramoyl-L-alanyl-D-
                     glutamyl-meso-2,
                     6-diaminopimelyl-D-alanyl-D-alanine-diphosphoundecaprenol
                     4-beta-N-acetylglucosaminlytransferase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0030259 - obsolete lipid glycosylation
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HIR1113639.1"
                     /translation="MRVLIAAGGTAGHINPALAIAGALKAADPTAEIHFAGRREGMEY
                     GLVTKAGYPFHHIEINGFQRHLSLENIGRNIVAIWHLALSGPRTRKILNEVNPDLVIG
                     CGGYVSGPIVRAAAKRSIKTAIHEQNAFPGVTNKLLAKEVNIVLAASADAVEKLGAPE
                     KTTVVGNPVRPEVLTADRATARAKLNAGDRTVILSFGGSLGADRINQVVADLCAWEKQ
                     TNQH"
     gene            complement(808..2088)
                     /locus_tag="ACZPAX_06780"
     CDS             complement(808..2088)
                     /locus_tag="ACZPAX_06780"
                     /inference="COORDINATES: protein motif:HMM:NF013279.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0015648 - lipid-linked peptidoglycan
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HIR1113640.1"
                     /translation="MNTPSSLLGVLFDHTIGNILRMLAVIRLPKLERGRVSRGFVATL
                     IVIMGYGLVMLFSASYSSGYANYGDVYHFIRPQSIIAVAGLGVMWLVSNINYRALRYM
                     NESFYIITLVLLVAALFSPVDTNGTYRWVYFAGGAFSLQPSELAKFSVMLWTADMLDR
                     DNDKKKSLWYGLLKPALPLVPILVLLKMEPHNSAMMLLCLIFVTMIACNGSAMRWWPL
                     AGAGTVAGAIAYVKHLASGEGYAAGRLGGVWGLTPTNTAGMQWQTRQSIYAICTGGLF
                     GVGIGNSVQKHQWLPYAENDFVFAVIGEELGFVGAIALIALFAALVIQGIFIALRAPD
                     LYGTLLGIGIVSQVAWQVFLHIGVGTALLPNTGISLPFFSYGGTSLLLLLGEMGVLLS
                     ISRAGNAREERMAEQHRAETDRMLHRTRYRRGTV"
     gene            complement(2123..3133)
                     /gene="mraY"
                     /locus_tag="ACZPAX_06785"
     CDS             complement(2123..3133)
                     /gene="mraY"
                     /locus_tag="ACZPAX_06785"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HIR1113641.1"
                     /translation="MEEISYLLASAAIGGFVITAVSGKFMIPALHRLHFGQTVREEGP
                     TWHNKKNGTPTMGGLCFIFGILVTLGLVWGMFHNQLPEVLGLPQMKAGMMVLFLAFGS
                     GLIGFLDDFIKVIKHRNLGLTEAQKMILQIALTFGFMFGLNSLGLLTTQVQLPIFGLV
                     DMGLVYYPIAFFGIIFLVNAVNLTDGLDGLCTGVTFVSMIGYLLAGSLLGFVHVSLIA
                     AATAGACAGFLVWNFYPAKVFMGDTGSMFFGGIVTALAFVMQRPELVLFFGIVYIWDA
                     MTVVIQRVYFKATHGKRIFRMTPIHHAFEMRGWREVKIDAFFALIAIIGVAMGIFYIC
                     VA"
     gene            complement(3263..>4289)
                     /locus_tag="ACZPAX_06790"
     CDS             complement(3263..>4289)
                     /locus_tag="ACZPAX_06790"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HIR1113642.1"
                     /translation="KEFCYAVLSAFGKTLKTEGNQNNDIGVPNTLFRLDNDTEYAVVE
                     MGMDHAGEIERLTRCARPSAGIITMIGVSHLENLGTRENILKAKMEICTGLPDGAPLV
                     LNADNDLLPTADVPTRLKAVWFGIDAENADVRAVDVETGTNGTTFKIIDKEYGTFNAA
                     IPTAGVHTVYDALAAYAAATRLGLDAARCAAALATYQTTGMRQHIVKKGGVTFIEDCY
                     NASPDSMRAALSVLKALPNTRKIALLGDMLELGDASEDGHRHTGEWAADAGVDQLIAY
                     GPRSAAMADAAKAHGVVTVHCKNAEEVLQYLRQFVRPGDAVLAKASHAMGLEQLLQDF
                     YKELPQG"
BASE COUNT          914 a         1273 c         1282 g          820 t
ORIGIN      
        1 tgtgttggtt cgtctgcttt tcccaggcac agaggtcggc cacgacctgg ttgatgcggt
       61 ctgcacccag gctgccgcca aagctcaaaa tgaccgtgcg gtcgcctgcg ttcagttttg
      121 cgcgggctgt ggcgcggtcg gcggtcagga cctcggggcg aaccgggttg cccacaacgg
      181 tggttttctc gggtgcgccc agcttttcta cggcatcggc gctggcagcc agaacgatgt
      241 tgacctcctt cgccagcagc ttgtttgtca cgccggggaa ggcgttctgc tcatggatgg
      301 cagtcttgat gctgcgtttg gccgccgcgc gcacaatagg gccgctgacg taaccgccgc
      361 agccgatgac aaggtcggga ttgacctcgt tcaggatttt ccgggtgcgg gggccggaca
      421 gcgccagatg ccagatggcg acgatgttgc ggccgatgtt ttccaggctg aggtggcgct
      481 ggaagccgtt gatttcgata tggtggaagg ggtaacccgc cttggtgaca agtccgtact
      541 ccatcccctc ccggcggccc gcaaagtgaa tttccgccgt cgggtctgcg gctttcaagg
      601 caccggcgat tgccaacgcg gggttgatat gaccggctgt accgccggca gcaatgagaa
      661 cacgcatgat tcggcctccc taaaattata agtttatgct aacggcaagc gggcaaagct
      721 tcactggtca tcgctggcac tcgaccacct cccctgtaag gggaaggctt acggggcgtc
      781 gaggacgccg ccccctacag cacggtctta caccgtacct ctgcggtagc gggtgcggtg
      841 gagcatacgg tcggtctcgg cgcggtgctg ttcggccatg cgttcctccc gcgcgttgcc
      901 cgcacgggat atactcaata gcacgcccat ctcacccagc agcagcaaca aactggtgcc
      961 gccgtaggag aagaacggca aactgatacc cgtgttgggc agcaacgccg taccgacgcc
     1021 gatgtgcaga aaaacctgcc acgccacctg ggacacaatg ccgatgccca gcagcgtacc
     1081 gtacaaatcg ggcgcacgca gcgcaatgaa gattccttgg atgaccagcg ccgcaaacag
     1141 ggcaatcagg gcaatcgccc cgacgaagcc cagttcctcc ccgatgacgg caaagacgaa
     1201 gtcgttctcc gcgtagggca gccattggtg tttctggacg ctgttgccga tgcccacacc
     1261 gaacagtcct ccggtacaga tagcatagat gctctggcgg gtctgccact gcatacccgc
     1321 cgtgttcgtg ggggtcaggc cccacacgcc gcccaggcgg cccgcggcgt agccctcgcc
     1381 cgaggcaagg tgcttcacgt aggcaatggc gcccgccacg gtacccgcac cagccagcgg
     1441 ccaccaccgc atggcggagc cgttgcaggc aatcatggta acaaaaatca ggcacaaaag
     1501 catcatggcc gagttgtgcg gttccatttt cagcagcacg agaatcggca ccagcggcag
     1561 cgcgggtttg agcagaccgt accacagcga ctttttctta tcgttgtcgc ggtcgagcat
     1621 atcggcagtc cagagcatga ccgaaaattt tgccagctca gacggctgca gcgagaacgc
     1681 accgcccgca aagtagaccc agcggtaggt accgttggtg tccaccgggc tgaacagcgc
     1741 cgccaccagc agcacgagcg tgatgatata aaaactctcg ttcatgtagc gcagggcacg
     1801 gtagttgatg ttggacacca gccacataac gcccaggccc gccactgcaa tgatgctttg
     1861 cgggcggatg aagtggtaga catcaccgta gttggcgtac ccgctggaat agctagctga
     1921 aaacagcatg accagcccat atcccataat gacgatcagg gtcgccacga aacctcggga
     1981 gaccctgccg cgttcgagtt ttggcaggcg gatgaccgcc agcatacgca gaatattacc
     2041 gatggtgtgg tcaaacagca cccctaacag gcttgacggc gtattcacag gcagtcacct
     2101 ccttagcaga ttttgcgcgg tgttacgcca cgcagatgta gaaaataccc atagccacac
     2161 caataatggc aatcagggca aagaaagcgt caattttgac ttcgcgccag ccgcgcattt
     2221 caaaggcgtg gtggatcggc gtcatgcgga agatgcgctt gccgtgggtc gccttgaagt
     2281 agacgcgctg gataacaacg gtcatggcgt cccagatgta cacgataccg aagaagagca
     2341 ccagttcggg gcgctgcatt acaaaggcca gcgccgtgac gatgccgccg aagaacatac
     2401 tgccagtatc gcccatgaac accttggccg ggtagaagtt ccagaccagg aacccggcgc
     2461 aggcacccgc cgtggcggcg gcgataagcg agacatggac aaagcccagc aggctgcccg
     2521 ccagcaggta gccgatcatc gacacaaagg tcacaccggt gcaaaggccg tcaaggccgt
     2581 ccgtcaggtt gacggcgttg accaaaaaga tgatgccgaa aaacgcgatg ggatagtaga
     2641 caagacccat atccaccaaa ccgaaaatcg gcagctgcac ctgggtggtg agcaggccca
     2701 ggctgttgag gccgaacata aagccaaagg tcagggcaat ctgcaagatc atcttttggg
     2761 cttcggtaag gccgaggttg cggtgcttga tgaccttgat aaagtcatca aggaagccga
     2821 tcagccccga gccaaacgcc agaaacagca ccatcatgcc agccttcatc tggggcaggc
     2881 ccagaacttc cggcagctgg ttgtggaaca tcccccacac caggcccagc gtgaccagga
     2941 tgccgaagat gaagcacagg ccgcccatgg tgggggtgcc gtttttcttg ttgtgccagg
     3001 tggggccttc ctcgcggacg gtctggccga agtgcagccg atgcagcgcc gggatcataa
     3061 acttgcctga caccgccgtg ataacaaagc cgccgatggc cgccgacgcc aacagatagg
     3121 atatttcctc catgcagtga ttcctccaaa atgatgtggt gcagggggat ttgggaattt
     3181 acgctgttta cagacaacta tgggcagacc agcccctcat cagtccgctg cgcggacagc
     3241 ttttcccgaa gggggaagcc ctttacccct gcggcaattc tttatagaaa tcctgaagca
     3301 gctgttccag ccccatggcg tggctggcct tggccaggac tgcgtcaccg gggcgcacga
     3361 attgccgtag atattgtaac acttcttcgg cgtttttgca atgcacagtt acaacaccgt
     3421 gcgcttttgc ggcgtctgcc atggcagcgc tgcgcgggcc gtaggcaatc agctggtcaa
     3481 cacccgcgtc ggcagcccac tcaccggtgt ggcggtggcc gtcctcgctg gcgtcgccca
     3541 gttccagcat atcgcccagc agggcaattt tgcgggtgtt gggcagggct ttcagcaccg
     3601 agagcgcggc tctcatgctg tcggggctgg cgttgtagca gtcctcaatg aacgtaacac
     3661 cgcctttttt tacaatatgc tggcgcatac cagtcgtctg gtatgtggca agggccgctg
     3721 cacagcgggc cgcatccagc cccaggcggg tggcagcggc ataggcagcc agcgcgtcat
     3781 agacggtgtg aacgcctgca gtgggaatgg cggcattgaa agtgccgtat tctttatcaa
     3841 tgatcttgaa cgttgtaccg ttcgttccgg tctcaacatc cacggcgcgg acatcagcgt
     3901 tctcggcatc gataccgaac cagacggcct tcagcctggt aggcacatcg gcggtgggca
     3961 gcaggtcgtt gtcagcgttg agcaccagcg gcgcaccatc gggcaggccg gtacaaatct
     4021 ccatctttgc tttcagtata ttctcccgag tgccgagatt ttccaggtgg gagacgccta
     4081 tcatcgtgat gatgcccgcc gacggccgtg cgcaacgggt caggcgctcg atctcgcccg
     4141 catgatccat gcccatttcg acgacggcgt actcggtatc gttgtccagg cggaacagcg
     4201 tattgggcac gccgatatcg ttgttctggt tgccctcggt tttcagcgtc ttgccaaagg
     4261 cggacagcac ggcgtaacag aactctttc
//
LOCUS       DBMDSQ010000168         4286 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_4822_length_4286_cov_2.234409, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000168 DBMDSQ010000000
VERSION     DBMDSQ010000168.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 4286)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4286)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4286
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4822_length_4286_cov_2.234409"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(170..1405)
                     /locus_tag="ACZPAX_06795"
     CDS             complement(170..1405)
                     /locus_tag="ACZPAX_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015537785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase type-2 family protein"
                     /protein_id="HIR1113643.1"
                     /translation="MDVFLRKDADAIISASLRAVLPDEAVHRALQTFQPRGGRVVLVA
                     AGKAAWQMAQAAVETLGRVDGGVVITKYGHVKGALPGVTCCEAGHPVPDANGFAATEQ
                     ALAVVRGLTAADTVLFLLSGGGSALFEQPLLPSTELQDITNQLLACGADIVEMNTVRK
                     RLSGVKGGRFAQACAPAQVFSIVLSDILGDPLDMIASGPAAPDSSTCAQALAVAEKYH
                     LTLSTQARALLGRETPKELTNVTTQITGSVRQLCAAAADACRALGYEPVLLTDRLCCE
                     AREAGSFLGAIARTHAGQGRRLAFIAGGETVVHLTGHGLGGRNQELVLAAAPALDGLH
                     AAVFAVGSDGTDGPTDAAGGYVDGGTLAALTAHGLTVFDTLQKNDAYHALQAAGGLIL
                     TGPTGTNVNDVAVALVAGD"
     gene            complement(1433..2077)
                     /gene="pcp"
                     /locus_tag="ACZPAX_06800"
     CDS             complement(1433..2077)
                     /gene="pcp"
                     /locus_tag="ACZPAX_06800"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004065983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016920 - pyroglutamyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HIR1113644.1"
                     /translation="MKILLTGFDPFDREKVNPAFEAVKLLPDTIAGAQIVKLEVPTQF
                     VRAGEVLEAAMAEHKPDVVICVGQAGGRAAITPEKVAINLMDGRIPDNAGYQPVDVPI
                     RADGPAAYFATLPVKAMVQRMQDAGIPAAVSYTAGTYVCNSLMYMLLFLIDRKFPSVR
                     GGFIHVPYAMEQAVNKPLGTPSMDLRQISRGLEKAVEAVVICGRDTAVPMGTTH"
     gene            2268..3104
                     /locus_tag="ACZPAX_06805"
     CDS             2268..3104
                     /locus_tag="ACZPAX_06805"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007048517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine kinase"
                     /protein_id="HIR1113645.1"
                     /translation="MSKLLLINDLAGYGKVALSAMIPILSHMQYEVCSLPTALVSNTL
                     DYGKFEILETTSYMKNTLQVWDALGFQFDAVSTGFIVSRPQTELVRDFCRAQKVKGTR
                     IFTDPIMGDEGKLYNGVGEETVALMRELIATADYIVPNYTEAAYLAGVPYQCGGTTRE
                     ELYALTDKLHALGAGSVLITSARMLVDGGWKPIACVVGYDAAADQHFILPYEELPVRF
                     PGTGDIFSAVFMGHILRGDALKTAAQAAMQTVSKMLAQNADNQDKYKGIPLETCLEDV
                     VL"
     gene            3101..3379
                     /locus_tag="ACZPAX_06810"
     CDS             3101..3379
                     /locus_tag="ACZPAX_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009260542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04076 family protein"
                     /protein_id="HIR1113646.1"
                     /translation="MSRPRVKITLIDRKGPVGCHRGHRVGDSWDFDTQRGEICPMAMH
                     VAFLYIDILRYGGSIPGQNPGEAIFSCPDADTLNIFKVEVVQGESLLP"
     gene            complement(3429..>4286)
                     /locus_tag="ACZPAX_06815"
     CDS             complement(3429..>4286)
                     /locus_tag="ACZPAX_06815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113647.1"
                     /translation="TTETSFTVPNGAVGYPTDPYHDYDPYQQENVPEDAAFIFGPGDA
                     EVSITGVTRSDPDADGSVTYTVAFDTTATLRYAFTKALLDDGTITELGMGRCFAGFGL
                     LDAQTGTVFAPQQGAGNWPWSVPYLGYNSYTVVMRPQAEADKKYSDAPSPTREDPCQQ
                     VRPLTSYTLDDGRTCRLGFAEVGSWDFGETTGGQGNANGKTWALFINKSTMHGVLQIK
                     VDAGYDDLVLYYNAQGFDENDAFAYDLWAGDGEDGPLYLDELGYFKGMQGMRFYRLDE
                     LVSLFAEKA"
BASE COUNT          802 a         1403 c         1298 g          783 t
ORIGIN      
        1 gcctcccggg gtcggggtct tgtggatata ttcccattgc aaacccgctt gccctgtgcc
       61 gcctggacta cacgatgagc gaggccaatg gtgtggccaa ctttgtacgg cagtacacgc
      121 cggcctccgg cgggccgtac tgcgactgcg gctacaagaa aaaagcgctt taatccccgg
      181 ccaccagcgc cacggccacg tcgttgacgt ttgtgccagt ggggcctgta agaatcaatc
      241 cgcctgccgc ctgcaaagcg tggtaggcgt catttttttg cagcgtgtca aacacggtca
      301 ggccgtgggc ggtcagcgcg gccagcgtgc cgccgtccac atagccgcct gcggcatcgg
      361 tggggccgtc ggtgccgtcg ctgcccaccg cgaataccgc cgcgtgcagg ccatccagcg
      421 ccggggccgc cgccaggaca agctcctgat tgcgcccgcc caagccgtgc ccggtcaggt
      481 ggaccacagt ctcgccgccc gctataaagg ccaaccgccg cccctgcccg gcgtgggtgc
      541 gggcaatcgc accgaggaaa ctgcccgcct cccgcgcctc gcagcagagc cggtcggtca
      601 gcagcaccgg ctcgtacccc agcgcccggc aggcgtcggc tgcggcggcg caaagctgcc
      661 gcacactgcc ggttatctgc gttgtaacgt tggtcaattc cttcggcgtc tcccggccca
      721 gcagcgcccg ggcctgagta gagagggtca aatggtactt ttccgccacg gccagcgcct
      781 gggcgcaggt ggaggaatct ggcgcagccg ggccgctggc gatcatatcc agcgggtcgc
      841 cgagaatgtc gctcagcacg atgctgaaca cctgcgcggg ggcgcacgcc tgggcaaacc
      901 gcccaccctt cacgcccgaa agccgcttgc ggactgtgtt catctcgacg atatctgccc
      961 cgcaggccag cagctgattc gtgatgtcct gtaattccgt ggaggggagc agcggctgct
     1021 caaacagcgc actgccgccg cctgaaagca aaaacagcac ggtgtccgcc gccgtcagcc
     1081 cacgcaccac cgccagcgct tgttcggtgg cggcaaagcc gttcgcgtcg gggaccgggt
     1141 ggcccgcctc gcagcaggtc acgccgggca gtgcgccctt gacgtggccg tacttggtga
     1201 tgaccacgcc gccgtccacc cggcccagtg tttccaccgc ggcctgtgcc atctgccacg
     1261 cggccttgcc cgccgcaacc aacacgaccc gcccgccccg tggctggaag gtctgcagcg
     1321 cccggtgcac cgcttcatcc ggcagaaccg cccgcagact ggcagaaata atcgcgtcag
     1381 cgtcctttcg caggaacaca tccatgggat tgacctcctt gtgcggccat gatcaatgag
     1441 tcgtgcccat gggcacggcg gtgtcgcggc cgcatattac cacggcttcc acggcctttt
     1501 ccagcccgcg ggagatttgt cgtaaatcca tgctgggggt gccaagcggc ttgttgacgg
     1561 cctgctccat ggcgtagggc acatgaatga acccgccccg cacgctggga aatttccggt
     1621 cgataaggaa aagcagcatg tacatcagtg aattgcacac ataggtgccg gcagtgtagg
     1681 acacggcggc ggggatgccc gcgtcctgca tccgctgcac catggctttg accggcagcg
     1741 tggcaaagta ggcggctggc ccatcggccc ggatgggcac atccacaggc tgatacccgg
     1801 cgttgtccgg gatgcggcca tccattaggt tgatagcgac cttctccggc gtgatggccg
     1861 cgcgcccgcc cgcctgcccg acgcagataa ccacatcggg cttatgttcc gccatggcgg
     1921 cttccagcac ctcgcccgcg cggacaaact gggtggggac ttcgagcttg acgatctgtg
     1981 ccccggcaat cgtgtctggc agcagcttca ctgcctcaaa cgccgggttg accttctccc
     2041 tgtcgaacgg atcaaagcca gtgagtagga ttttcatgga ggaaacctcg tttcgcattt
     2101 tttattatta tatcataggt tgcccccgcc cgcaaagcat gctacaatac aggcatgtaa
     2161 cccttttatc tgtaaagttg tagggaggat ggttgatctc tctgcggagc gtcgaggacg
     2221 ttgctcctta cacatgccaa catatacaac aaaggatggt ctcctctatg agtaaactgc
     2281 ttctcatcaa cgacttggcc gggtacggca aggtcgcgct cagcgctatg atccccattc
     2341 tctcccatat gcagtacgag gtttgcagcc tgcctaccgc gctggtcagc aacacactgg
     2401 actacggcaa gtttgagatc ctcgaaacca ccagctacat gaaaaacacc ctgcaagtct
     2461 gggacgcgct gggctttcag ttcgacgcag tgtccaccgg attcatcgtc tcccgcccgc
     2521 agaccgaact ggtgcgggac ttctgccgcg cgcaaaaagt caaaggaacg cggattttca
     2581 cagaccccat catgggcgac gagggcaaac tctacaacgg cgtcggcgaa gaaaccgtcg
     2641 ccttaatgcg ggaactgatt gccacggcgg actacatcgt gccaaactac accgaggccg
     2701 cctacctggc gggcgtgccc taccagtgcg gcggcaccac gcgggaggaa ctgtacgccc
     2761 tgaccgacaa gctgcacgcc ctgggtgcgg ggtcggtgct cattaccagt gcacggatgc
     2821 tggtggacgg cggctggaag ccgattgcct gcgtcgtcgg ctacgacgcg gcagcagacc
     2881 agcactttat tctgccctac gaggaacttc ccgtgcggtt ccccggcacc ggtgatatct
     2941 tctcggcggt gttcatgggg cacattctgc gcggtgacgc gctgaaaacc gccgctcagg
     3001 ccgccatgca gaccgtgagc aaaatgctgg cccaaaacgc cgacaaccag gacaaatata
     3061 agggcatccc actggagacc tgcctggagg atgtggtgct atgagccgcc cgcgcgtgaa
     3121 aatcaccctc atcgaccgca aaggcccggt gggctgccac cgcggccacc gggtgggcga
     3181 cagctgggac ttcgacaccc agcgcggcga gatctgcccc atggcgatgc acgtcgcatt
     3241 tttgtacatc gacatcctgc gctacggcgg cagcatcccg ggccaaaacc ctggtgaggc
     3301 aattttcagc tgccccgacg ccgacacgct gaacattttt aaagtcgagg tcgtgcaggg
     3361 agaaagcctc ctcccgtaag cagggaggtg gccgaaggag ctttgccccg gggctgggat
     3421 gccgccccct acgccttctc ggcgaacagg gaaaccagct cgtccaagcg ataaaaccgc
     3481 atcccctgca tccccttaaa ataccccaat tcatccagat acagcggccc atcctcgccg
     3541 tcaccggccc acaggtcata cgcaaacgcg tcattttcat caaagccctg ggcgttataa
     3601 tacagcacca aatcgtcata cccggcatcc acttttatct gtaaaacgcc gtgcatcgtg
     3661 cttttgttga tgaaaagggc ccaggtcttg ccgttggcat tgccctggcc gccagtcgtc
     3721 tcgccaaaat cccagctgcc tacctcggca aagccaaggc ggcaggtgcg gccgtcgtcc
     3781 agcgtgtagc ttgtcagcgg gcgcacctgc tggcagggat cctcccgcgt ggggctgggg
     3841 gcgtcgctgt attttttgtc ggcctcggcc tgtgggcgca tgacgaccgt gtagctgttg
     3901 taccccaggt acggaacgct ccacggccag ttgcccgcgc cctgctgcgg ggcgaacact
     3961 gtgccggtct gggcgtccaa caggccaaag cccgcaaagc agcggcccat gcccagctcg
     4021 gtgatggtgc cgtcgtccag caaggccttg gtaaaggcgt agcgcagcgt ggccgtggtg
     4081 tcaaaagcga ccgtgtaggt cacgctgccg tcggcgtcgg ggtcgctgcg ggtcacgccg
     4141 gtgatgctca cctccgcgtc cccgggaccg aagatgaacg cggcgtcctc gggaacgttc
     4201 tcctgctggt aggggtcgta gtcgtggtag gggtcggtgg gatagcccac cgcgccgtta
     4261 ggcacggtga agctcgtctc ggtggt
//
LOCUS       DBMDSQ010000169         4286 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_4824_length_4286_cov_1.857109, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000169 DBMDSQ010000000
VERSION     DBMDSQ010000169.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 4286)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4286)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4286
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4824_length_4286_cov_1.857109"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1600)
                     /locus_tag="ACZPAX_06820"
     CDS             complement(<1..1600)
                     /locus_tag="ACZPAX_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007493406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HIR1113648.1"
                     /translation="MKRLVIGLLAHVDSGKTTLAEGLLYRAGVLRKLGRVDHRDAFLD
                     TDSQERARGITIFAKQAVLTLPAADGCGETALTLLDTPGHVDFSAEAERALQVLDYAV
                     LVVSGTDGVQAHTETLWKLLARYRVPTFVFVNKMDLPGADAAVRLRELRGRFGDGCVD
                     FSTAPDPETLALCSEPLMNEVLDTGTPASETVITAIARRQVFPVFFGAALRLDGLDGL
                     LRGLQTLTRTPPQWPEFGARVFKISEDAGTRLTWLKVTGGVLHVKDVLPGGEKADALR
                     LYNGGKFRLVSEAFPGMVVAVAGPAATRPGQGLGAESDAETPLLEPVLNYRIDCDADA
                     HTVLKALQTLEDEDPQLHVNWRDDLGEVHVQLMGEVQLEILQNILQDRFGLDVTFNEG
                     GILYKETLTTAVEGIGHYEPLRHYAEVHLLLEPGARGSGIQLAADCPPDTLAENWQRL
                     ILTHLAERTHPGVLIGAPLTDVKITLAAGRAHQKHTEGGDFRQATYRAVRQGLRMAAA
                     KDGVTLLEPWYDFTLELPADALGRA"
     gene            1920..3122
                     /locus_tag="ACZPAX_06825"
     CDS             1920..3122
                     /locus_tag="ACZPAX_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015564934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR1113649.1"
                     /translation="MNLQKQLRCVYLHAFFSTLRFTDAVWVALLAARGFSLAEIGLAE
                     GVFHLTSLLCEVPSGMAADLLGRRRTLIFGGVLGVFSALAMASAPGLGLICTAMALKA
                     LGYNMLSGTAEALIYDSLKMAGREKDYIKVDANASICMKIATALGSLASLLAGVLRYA
                     GYYLADAVSALLSALAAAQLEEPIVTDEQAARERHTLRELPARLRVHILDSADCLRGS
                     ALARRLIAADAVISLPSYLTSMFVQQRLTEQGGAMEWLFLPGLLAGAAGMAGTALGRR
                     LHPKTLRGLYLACALTIGAGALLAGAAPAWACAVGPMLVQGALAVWFLHSMQRMNESI
                     SSDRRATLISVDSMTYSLLMIPASPMLGWLGDAAGSAGVGLCLLGAMVAASGLAVAAK
                     TRYNTRGA"
     gene            3185..4204
                     /locus_tag="ACZPAX_06830"
     CDS             3185..4204
                     /locus_tag="ACZPAX_06830"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015536924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HIR1113650.1"
                     /translation="MTYTEVLQAAKGQMGFCHACPVCNGRACGGTIPGPGAKGSGTVA
                     IRNFDAWRNVRLNMDTIHENFEPDTGLELFGRKFKYPFFAGPVGAMTLHYGDKYNDLQ
                     YNEILVAACAAAGIAAFTGDGTNPKVMEGATAAIAASGGAGIPTVKPWDNATVAEKMT
                     MARQAGCFAMAMDIDAAGLPFLQHLDPPAGSKTVEQLQEIAQAAGVPFILKGIMTVRG
                     AEKAIEAGAVGIVVSNHGGRVLDDCPATEEVLADIVAAVDGRAKILVDGGIRSGNDVF
                     KALALGADGVLVARPFVTAVYGGGADGVAAYVEKLGAELADTMRLCGAASLADISRDM
                     VRVVK"
BASE COUNT          742 a         1229 c         1497 g          818 t
ORIGIN      
        1 tggcgcggcc cagcgcgtcg gcgggaagtt ccagcgtgaa gtcataccac ggttccagca
       61 gcgttacgcc gtccttggct gcggccatcc gcaggccctg gcgcacggcg cggtaggtgg
      121 cctggcggaa gtcgccgccc tcggtgtgct tctggtgggc gcggcccgcc gccagcgtga
      181 tttttacgtc ggtcagcggc gcaccgatca gcacgccggg gtgggtgcgc tcggcaagat
      241 gggtcaaaat cagccgctgc cagttttcgg ccagtgtgtc gggcgggcag tctgccgcca
      301 gttgtatgcc gctgccccgc gcaccgggtt ccagcaacag gtggacctcg gcgtaatggc
      361 gcagcggctc atagtggccg atgccctcca cagcggtggt cagggtttct ttgtagagga
      421 taccgccctc gttgaaagtg acgtccagcc cgaagcggtc ctgtaaaata ttctgcaaaa
      481 tttccagctg cacctcgccc atcagctgga cgtgtacctc gcccaggtcg tcgcgccagt
      541 tgacgtggag ctgcgggtcc tcgtcctcga gggtctgcag ggcttttaaa acggtgtggg
      601 cgtcggcgtc acagtcgatg cggtagttga gcacaggctc caaaagcggc gtctccgcgt
      661 cggattccgc accaaggccc tgcccggggc gggtggctgc cgggcctgcc actgccacga
      721 ccatgccggg aaacgcctca ctgaccaggc ggaacttgcc gccattgtac agccgcaggg
      781 cgtcggcctt ttcgccgccg gggagcacgt cttttacgtg cagcacgccg ccggtgactt
      841 tcagccaggt caggcgggtg cctgcgtcct cactgatttt gaacacgcgt gcgccaaatt
      901 ccggccactg gggtggggtg cgggtcagcg tttgcaagcc gcgcagtaag ccgtccaggc
      961 cgtccagacg cagcgccgcg ccgaaaaaca ccggaaagac ctgccgccgc gcaatggccg
     1021 tgatgacggt ctcggacgcg ggggtgccgg tgtcgaggac ttcgttcatc agcggctcac
     1081 tgcacagggc cagggtctcg gggtcgggcg cggtggaaaa atccacacag ccgtcgccga
     1141 agcggccccg cagctcccgc aggcggacag cagcgtccgc gccgggcaga tccattttgt
     1201 tgacaaaaac aaacgtagga acgcggtagc gggcaagcag cttccacagt gtctcggtgt
     1261 gggcctgcac gccgtcggtg ccggagacga cgaggacggc gtagtccagc acttgtaagg
     1321 cgcgctcggc ctcggcggag aaatccacgt ggccgggagt gtccagcagg gtcagcgcgg
     1381 tctcgccgca gccgtcggcg gcgggcagcg tcagcaccgc ctgtttggca aaaatcgtga
     1441 tgccccgcgc ccgctcctga ctgtccgtgt ccagaaaggc gtcacggtgg tcgacgcggc
     1501 ccagcttgcg cagcacgccg gcgcggtaga gcagcccttc ggccagtgtg gttttgccgc
     1561 tgtcaacatg ggccagcagg ccgatgacga gacgtttcat agatgtttcc cccgagcttt
     1621 tcaaagttgc ttctattgta caatttttct tgcaatttgg caaacggtgt ggtatagtaa
     1681 agccaaattt accgggaagg agagcacacg tatgcgttgg cagttcggtg tggtgttcca
     1741 gaaaaaagga aacccctgac gagttgacgc tgtaagtgtg ccctttttta ggtattgccc
     1801 taagttccgg taaagctata agtgtgtgca tttttgcgcc ctgcggcttt gctgcggggc
     1861 gctttttgtg tgcaaaaaag ggcacgtttt cgtgattttt gagcgaaagg gtgttcccga
     1921 tgaatttaca aaaacaactg cgctgcgtgt atctgcacgc atttttctcg acactgcggt
     1981 ttaccgatgc cgtctgggtg gcgctgctgg cggcgcgggg cttctcgctg gcggaaatcg
     2041 gcctggccga aggggtgttc catctgacga gcctgctgtg cgaggtgccc agcggcatgg
     2101 ctgcggatct gctggggcgg cggcgcacgc tgattttcgg cggtgtgctg ggcgttttct
     2161 cggcgctggc catggcgtct gcaccggggc tggggctgat ctgcacggcc atggcgctga
     2221 aggcgctggg ctacaatatg ctttccggca cggccgaagc cctgatctac gacagcctga
     2281 aaatggccgg gcgggagaaa gactatataa aagtggacgc caatgcgtcg atttgcatga
     2341 aaattgcaac ggcgctcggc tcgctggcaa gcctgctggc aggggtgttg cgctacgcag
     2401 ggtactactt ggcggacgcc gtgtcggcgt tgctctcggc actggcggcg gcccagctgg
     2461 aagaacccat cgtcaccgat gagcaggccg cccgggagcg gcacaccctg cgggaactgc
     2521 ccgcccggct gcgtgtgcat attttggaca gcgccgactg cctgcgcggc agtgcgctgg
     2581 cacggcggct gattgcggcg gacgcggtga tttcgctgcc aagctacttg acgtcgatgt
     2641 ttgtgcagca gcggctgacg gaacagggtg gggccatgga atggctgttc ctgccggggt
     2701 tgctggccgg ggctgcaggt atggcgggca cggcactggg gcggcggctg caccctaaga
     2761 ccctgcgcgg cctgtattta gcctgtgcgc tgaccattgg cgcaggggca ctgctggccg
     2821 gggcggcccc ggcgtgggcc tgtgccgtcg ggccgatgct ggtgcagggg gcactggctg
     2881 tgtggttctt gcacagtatg cagcggatga acgagtcaat ttccagcgac cgccgcgcga
     2941 cgcttatcag cgtggacagc atgacgtaca gtttgctgat gatcccggca agcccgatgc
     3001 tgggctggct gggggatgct gccgggtccg caggggtcgg gctgtgtctg ctgggggcaa
     3061 tggtggcagc cagcggtctt gcagtcgcgg ccaaaacacg gtataataca cgcggagcat
     3121 aattcccttg attttttgtg gggtcgatgg gatgcgtcgg cccgccaacg gaggcaaaat
     3181 tgctatgacc tatacagaag ttttgcaggc ggcgaagggc cagatgggct tttgccatgc
     3241 ctgcccggtg tgcaatggcc gcgcctgcgg tggcaccatc cccggcccgg gggccaaggg
     3301 cagcggcacg gtggcgatcc gcaactttga cgcgtggcgg aatgtgcggc tgaacatgga
     3361 tacgatccac gagaattttg agccggatac gggcctggag ctctttggac gcaagttcaa
     3421 atacccgttt tttgcggggc cggtgggcgc aatgacgctg cactacggcg ataaatacaa
     3481 cgacttgcag tacaatgaaa ttttggtcgc tgcctgcgcg gcggcgggca ttgcggcgtt
     3541 caccggtgat ggcacgaacc ccaaggtcat ggagggagca acggcggcca ttgcggccag
     3601 cggcggcgca ggcatcccca ctgtgaagcc gtgggacaac gccacagtgg cggaaaaaat
     3661 gacgatggcg cggcaggccg gctgctttgc catggcgatg gacattgacg cagcgggcct
     3721 gccgtttttg cagcacctgg atccgcccgc cggcagcaag accgtggagc agctgcaaga
     3781 aatcgcccag gcggcgggcg tgccgtttat cttaaagggc atcatgacgg tgcgcggcgc
     3841 ggaaaaggcc atcgaggccg gggccgtggg catcgtcgtc tcgaaccacg gcggccgcgt
     3901 gctggacgac tgccccgcca ccgaggaagt gctggcggac attgtggcgg ccgtggatgg
     3961 ccgcgcgaaa attctggtgg acggcggcat ccgcagcggc aacgatgtgt tcaaggcgct
     4021 ggccctgggc gcggacggcg tgttggttgc ccgccccttt gtgacagctg tgtacggcgg
     4081 cggtgccgac ggcgtggctg cctatgtgga aaagctgggt gccgagctgg ccgacacgat
     4141 gcgcctgtgc ggcgcggcga gcctggcgga catcagccgc gatatggtca gggtcgtaaa
     4201 gtaaaggcaa taccgcataa ttctaagtgc cgatacggcg agcgaggtgc ggcagctgct
     4261 aagccaaaag cgcagataat actttg
//
LOCUS       DBMDSQ010000170         4284 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_4828_length_4284_cov_1.649534, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000170 DBMDSQ010000000
VERSION     DBMDSQ010000170.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 4284)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4284)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4284
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4828_length_4284_cov_1.649534"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..187)
                     /locus_tag="ACZPAX_06835"
     CDS             complement(<1..187)
                     /locus_tag="ACZPAX_06835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113651.1"
                     /translation="MLIFKVAKFALRHPLLTHGAKQLVHKKLRPKKEASPMKQKVRGH
                     AFRLVGLILGIIGATVSI"
     gene            complement(211..660)
                     /locus_tag="ACZPAX_06840"
     CDS             complement(211..660)
                     /locus_tag="ACZPAX_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005929301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HIR1113652.1"
                     /translation="MQKLKGFIKKYYEGLAYLFFGGVATLLNLVVFAAFQAAFGTDFA
                     AGIGNVIDNIICILFAYWTNHTFVFKSENKGKAALAEFGQFVSCRIATMVMDQLIIWV
                     GVSLVGPHVAFAAANGKLWAMGVKLFSQVIVILSNYVFSKLFIFKKK"
     gene            complement(793..>2200)
                     /locus_tag="ACZPAX_06845"
     CDS             complement(793..>2200)
                     /locus_tag="ACZPAX_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HIR1113653.1"
                     /translation="WENCKGFLKKECTKAKIFDTICNATWARQQEAEDLSQKCDRMVV
                     IGGHHSSNTQKLLQVAARHTKAINVETADELDPSWLAGAAVVGVTAGASTPSSIIEEV
                     LNSMSEIRDDMSFEEMLAASEAKPLYAGKIVKAKVISVSPTECTVGIDGSKHTGIVPL
                     REMSHDPNAKMEDLVKEGDDLDLVVVKTNDQEGVDTLSRVRFEAQKGMKDVSEAAENG
                     TVMEGDVMEANKGGVVVNVKGVRVFVPRSQATMRRDEDYTKLVGQHVQLVITECAGRK
                     IVGSINKVTAEANKAKREEFWANVEVGKQYKGVVKSLTSYGAFVDVGGVDGLCHISEL
                     SWNNIKHPSEVVKVGDEIEVYVKSYDPENQKVSLGYKKEEDNPWVKLENEVPVGTEFT
                     APVVSITKFGAFVRIMPGIDGLVHISEISNERVNKVSDVLKVGDEVRVKLTAVDFDRK
                     RISLSMKACLDENGEDAE"
     assembly_gap    2201..2210
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2211..2677)
                     /locus_tag="ACZPAX_06850"
     CDS             complement(<2211..2677)
                     /locus_tag="ACZPAX_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005944721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase/30S ribosomal protein S1"
                     /protein_id="HIR1113654.1"
                     /translation="MQIIRAKTAGFCFGVDRAVKLTYDLLAQGRKVATLGPLIHNAQV
                     VADLEEKGAVTCPDIDAVPDGYEVIIRSHGVPRTVYDKISTRRLAYHDATCPFVAKIH
                     KIAMEADKIGALLLVAGDADHPEVQGIVGHTTGPVRVFADLEQLQKLLPELLQ"
     gene            complement(2679..3392)
                     /locus_tag="ACZPAX_06855"
     CDS             complement(2679..3392)
                     /locus_tag="ACZPAX_06855"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009322090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HIR1113655.1"
                     /translation="MLLYWILLPIVWVLAHIIWRFEVIGRENLKAVRDGRAYVIAPNH
                     IANLDPVFVAITVFDWKRLRILAKEELFKNPLAGWFLRCMGAVAIERGKGDTTIVEKL
                     TNECKNGTGIMVFPEGTRTKTGKLGMIKSGAFVIAAAAGADMLPVRIIYGTKDGKMHL
                     FCKIRIVFGEAIPAEDLQIKDQSHKMAELRRMKNRLRDELEQLLADNAFTPQIAENPA
                     PAVDVPEMTDKPQLPKGDA"
     gene            complement(3404..4066)
                     /gene="cmk"
                     /locus_tag="ACZPAX_06860"
     CDS             complement(3404..4066)
                     /gene="cmk"
                     /locus_tag="ACZPAX_06860"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0036431 - dCMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HIR1113656.1"
                     /translation="MVSIAIDGPSGAGKSSLAKALAKDLGYVYVDTGAMYRSIGLYAV
                     RAGVDPHDADAVAALLPKIRLDIRLLDGAQHIYLNGEDVSDAIRAENIGMAASAVAAH
                     PAVRTFLLDTQRGLAASQNILMDGRDIGTVVLPNATVKIFLTASAEARAQRRAKELAE
                     KGQPADFATVLADIQQRDYQDSHRAVAPLKQAADAILVDTSDIGLQESFDLLKRTILA
                     HI"
BASE COUNT          854 a         1243 c         1210 g          967 t
ORIGIN      
        1 taatgctgac ggtagcgcca atgatgccaa ggatcaaacc aaccagacgg aacgcatggc
       61 cgcgcacttt ctgtttcata ggggatgcct cctttttggg acgtaatttt ttgtgcacca
      121 gctgctttgc tccgtgggtc agcagcgggt gcctcagtgc gaatttggct accttaaaaa
      181 taagcatggc tatctccttc ccggttcaag tcacttcttt ttaaagataa acaatttgct
      241 gaaaacatag ttggacaaaa tgacgatgac ctggctgaac agcttgacgc ccatcgccca
      301 cagcttgccg ttggcagcgg cgaaggcgac gtgcgggccg accaggctga cgcccaccca
      361 gataatgagc tggtccataa ccatcgttgc gatgcggcag gacacaaact gcccgaactc
      421 tgccagggcc gccttgcctt tgttctcgct cttgaacaca aaagtgtggt ttgtccaata
      481 ggcaaagagg atgcagatga tgttgtcgat gacattgccg atacccgccg caaaatccgt
      541 gccgaacgcc gcctggaagg ccgcgaacac gaccaggttc aagagcgttg ccaccccgcc
      601 gaagaatagg taggccaagc cctcgtagta ttttttgatg aagcctttga gtttttgcat
      661 gatgtacccg ttttatttgt aggggcgggc atcgcccacc cgcggggcgt cgagaacgcc
      721 gtctcctaca acgttcttat acaaaagggg cctgcctgtt acaacaggca ggccccttgc
      781 ggttgctcag acttattcag cgtcctcgcc gttctcgtcg aggcaggcct tcatggacag
      841 gctgatgcgc ttgcggtcga agtcaactgc ggtcagctta acgcgaacct cgtcgccaac
      901 cttcagcaca tcggaaacct tgttcacgcg ctcgttgctg atctcgctga tgtgaacgag
      961 accgtcaatg cccggcatga tgcggacgaa agcgccaaac ttggtgatgg agacaacggg
     1021 agcggtgaac tcagtgccaa cggggacttc gttctccagc ttgacccagg ggttgtcctc
     1081 ttccttctta tagcccaggg agaccttctg gttctcggga tcgtagctct tgacgtagac
     1141 ttcgatctcg tcgccgacct tgacgacctc agagggatgc ttgatgttgt tccagctcag
     1201 ctcgctgatg tggcacaggc catcgacacc gcccacgtca acgaaagcgc cgtaagaagt
     1261 caggctcttg acaacgccct tgtactgctt gccgacctca acattggccc agaactcctc
     1321 gcgcttggcc ttgttggcct cggcagtgac cttgttgatg gagccgacga tcttgcggcc
     1381 ggcacactcg gtgatgacca gctggacgtg ctggccaacc agcttggtgt agtcctcgtc
     1441 gcggcgcatg gtggcctgag agcggggaac aaagacgcga acgcccttga cgttgacaac
     1501 aacgccgccc ttgttggctt ccatgacatc gccttccatg acggtgccgt tctcggcagc
     1561 ctcggagacg tccttcatgc ccttctgggc ctcaaaacgg acgcgggaca gggtgtcaac
     1621 accctcctgg tcgttggtct taacgaccac cagatccaga tcgtcgccct ctttaacaag
     1681 gtcttccatc ttggcgttgg gatcatggct catctcgcgc aggggcacaa tgccggtgtg
     1741 cttggaacca tcgatgccga cggtgcattc cgtcggagaa acgctgatga ccttggcttt
     1801 tacaatcttg cctgcgtaca ggggctttgc ttcggatgcg gcgagcatct cctcaaagct
     1861 catgtcgtca cggatttcac tcatactgtt aagtacctcc tcaattatag acgaaggcgt
     1921 tgaagccccg gccgtcacgc ccaccaccgc agcaccggca agccagcttg ggtcgagttc
     1981 atcagcagtc tcgacgttga tggctttggt atgccgcgcg gcgacctgta acagcttttg
     2041 ggtattggaa gaatgatgac caccaatgac gaccatgcgg tcgcattttt ggctgaggtc
     2101 ttccgcttcc tgttgcctcg cccacgttgc attacatatt gtatcaaaaa ttttggcttt
     2161 tgtacactct ttttttaaaa aacctttgca attttcccaa nnnnnnnnnn tgttgcaaaa
     2221 gctcagggag caatttttgc agttgctcca aatcggcaaa aacccgcacc gggcctgttg
     2281 tatggcctac aattccctgc acttccgggt ggtcagcgtc gccagcgacc agcaaaagcg
     2341 caccgatttt gtctgcttcc atagcaattt tatggatttt tgctacaaac gggcaggtgg
     2401 catcgtgata cgccaggcgg cgtgtgctta ttttatcata gacagttctg ggcacgccat
     2461 gactgcggat gatgacctcg tacccatcgg gtacggcgtc gatatcgggg caggtcacag
     2521 cgcctttttc ttccaggtcg gctacgacct gggcgttgtg gatcagcggg ccaagggtcg
     2581 caactttgcg tccctgcgcc aataaatcat aggtcagctt gacagcgcgg tccacgccga
     2641 agcagaaccc ggctgttttt gcacggatga tttgcatatt atgcgtctcc ttttggcagc
     2701 tgcggcttgt cggtcatttc gggcacgtcc acggcgggag cggggttctc ggcaatctgc
     2761 ggggtgaacg cgttgtccgc cagcagctgc tccaattcgt cgcgcaggcg gttcttcatg
     2821 cggcgcagct cggccatttt gtggctctgg tctttaattt gcagatcctc ggcagggata
     2881 gcctcaccga acacgatgcg gattttacag aacaggtgca ttttaccgtc cttggtgccg
     2941 tagatgatgc gcaccggcag catatcggcc ccggctgcgg cggcaatgac gaacgcgccg
     3001 cttttgatca tgcccagctt gccggttttg gtacgggtgc cctcgggaaa gaccatgatg
     3061 cccgtaccat ttttgcactc attcgtcagc ttttccacga tggttgtgtc gcccttgccg
     3121 cgctcaatgg cgaccgcgcc catgcagcgc aggaaccacc cggccagcgg atttttaaag
     3181 agttcttcct ttgccaaaat gcgcagccgt ttccagtcaa acaccgttat agcgacgaaa
     3241 acagggtcaa ggttggcgat atggttcggc gcaatgacgt aagcgcggcc atcgcggacg
     3301 gctttcagat tctcgcggcc gatgacctca aaccgccaga tgatgtgcgc caggacccag
     3361 acaatgggca gcagaatcca gtataaaagc ataaacagcc ccctcagata tgcgcaagaa
     3421 tggtgcgctt cagcaggtca aagctctcct gcaaaccgat gtcactggtg tccaccagaa
     3481 tggcgtccgc agcctgtttc agcggggcga cggcgcggtg gctgtcctga tagtcgcgct
     3541 gctggatatc tgccagcacc gtggcgaagt cggcgggctg gcccttctcg gccaactcct
     3601 tggcgcggcg ctgggcgcgg gcctcggcgc tggcggtcaa gaaaatcttg acggtggcat
     3661 tcggcagaac caccgtaccg atatcgcggc catccatcag gatgttctgg ctggcggcca
     3721 gcccgcgctg ggtgtccagc aggaaggtac gcacggcggg gtgcgcagcc acggcggagg
     3781 cggccatgcc gatgttctcg gcacggatgg cgtcgctgac gtcctcaccg ttcaggtaga
     3841 tgtgctgtgc gccgtccaac aggcggatat ccagccggat cttcggcagc agtgcggcta
     3901 cggcgtcggc gtcgtgggga tccacgcccg cgcgcacggc gtacagcccg atggagcggt
     3961 acatagcgcc ggtgtcgacg tagacatagc ccagatcctt ggccagtgcc ttggccaggc
     4021 tggatttacc ggccccggac gggccatcga ttgcgataga aaccatgata tatcaccctt
     4081 tttaaataat aaatatcact ttggcttctc ccctgagggg agaagctgcc gcccgcaggc
     4141 ggctgatgag gggagcgttt tccggcacaa cccataaaca tgttgccgcc gtaaagttgc
     4201 gggccgggca tacccggccc ctaccgggtg gtttgcccat caacgggctg ccgccgtaag
     4261 gccgcccctc atccggccct gcgg
//
LOCUS       DBMDSQ010000171         4253 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_4876_length_4253_cov_1.535405, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000171 DBMDSQ010000000
VERSION     DBMDSQ010000171.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 4253)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4253)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4253
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4876_length_4253_cov_1.535405"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            136..1212
                     /locus_tag="ACZPAX_06865"
     CDS             136..1212
                     /locus_tag="ACZPAX_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HIR1113657.1"
                     /translation="MSSYIDVVHPDRHAPYLDIPNDVVEYLHYLDFVKMRSPRTINAY
                     YLDLRGFFRFMMAMQWSRVPRDTPLEEINLKDISTEDIKKITKRDIFSYLDYVRSTDA
                     NGGKARARKLSALKGFFSYLVTQVNKLQENPTEGISLGSPKKALPKYLTAAESIDLLK
                     NIQSDFYERDFCMITLFLNCGMRLTELVTINMGNFKQDTIRITGKGNKERLVYLNDAC
                     LFSLQRYKKARAALPNLADKDALFVSKRTGKRLSARRVEQIVARCLQSAGLSGRGFSP
                     HKLRHTAATLMYQGGVDMLALKEILGHENVSTTQIYTHINRDQLRQAVASSPLADKTF
                     EEQPSAPDLGSDEGEESPDGTENS"
     gene            complement(1150..1800)
                     /locus_tag="ACZPAX_06870"
     CDS             complement(1150..1800)
                     /locus_tag="ACZPAX_06870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113658.1"
                     /translation="MRAAQTTLRRPALLLPRRAERPRRECAGTAHSGAVWLFGTMFLA
                     GYLPGIWLGRGGVSALGQQLAAYYTKSPENAAVAAIFGTEFSVSFLQLTAVFLCGFCV
                     LGVGLLVLLFAARGVFLGYCAASVAAVNGAAGLVQYRLATIVSDAATLFFCLWLAGWS
                     ARLAGELFNVICGRATRETPGTLRRLAVRFGAALVLSAVFSAVGTFLAFVTAKVGR"
     gene            complement(1800..2156)
                     /locus_tag="ACZPAX_06875"
     CDS             complement(1800..2156)
                     /locus_tag="ACZPAX_06875"
                     /inference="COORDINATES: protein motif:HMM:TIGR04086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04086 family membrane protein"
                     /protein_id="HIR1113659.1"
                     /translation="MAVLLAFGCGVGACMVLLALGAFLLTHTGLPLAAVRPMACMAAA
                     IGAAVSARVLAGRLKQKLLLCGLGCGTFYTVCLMAATLAASGELDWQGANAMLPMALL
                     LGGLLGGALAALRGSR"
     gene            complement(2279..2629)
                     /gene="yajC"
                     /locus_tag="ACZPAX_06880"
     CDS             complement(2279..2629)
                     /gene="yajC"
                     /locus_tag="ACZPAX_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HIR1113660.1"
                     /translation="MIQFLDAATGTATTAEMLISFLPMILIIVFMVILIYLPQKRQDK
                     KDAAMRSSIEIGDKVTTIGGIVGIVCAIADKDDTIVIETGSDRTKIRFRRTAISSVEK
                     LDAGKDSKSTPVKK"
     gene            complement(2711..3844)
                     /gene="tgt"
                     /locus_tag="ACZPAX_06885"
     CDS             complement(2711..3844)
                     /gene="tgt"
                     /locus_tag="ACZPAX_06885"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HIR1113661.1"
                     /translation="MPYRLIKQEGAARRGEFTTVHGTVQTPAFQNVATAAAIKGGLSA
                     LDLKDIRAQVMLCNTYHLHLRPGDKLVAEMGGLHKFTKWDGPILTDSGGFQVFSLAKL
                     RHITEEGVTFNSHLDGHRIFMGPEQSMQIQANLGSTIAMAFDECVENPATYEYAKNSC
                     ARTARWLLRCKDEMNRLKHEEKAVNPDQLLFGINQGCTFEDLRVEHMKQIAEYDLDGY
                     AIGGLAVGEPAEVMYDVISAVEPFAPKNKIRYLMGVGTPGNIIEGVYRGVDLFDCVMP
                     SRNARHGHLFTWDGIINIKNLKYERDESPIDPHCDCPVCRNFSRAYIRHLQKADELLG
                     MRLAVMHNLYFYNHLMERIREALDNGTFQQFHDTYVHKLDTRI"
     gene            complement(3991..>4253)
                     /locus_tag="ACZPAX_06890"
     CDS             complement(3991..>4253)
                     /locus_tag="ACZPAX_06890"
                     /inference="COORDINATES: protein motif:HMM:NF018184.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HIR1113662.1"
                     /translation="LECAKVKKPNPLLEEIRFEIVQGGTCVQILHIGAYDDEPVSFAM
                     LDKFTRALSLIRAESCHREIYLNNANRTEKSKLKTILRYMVK"
BASE COUNT          989 a         1258 c         1141 g          865 t
ORIGIN      
        1 ccggcaccac ctgacagcag gcagaaaaaa gccccgcgct tgacacaggg gaagcagata
       61 gctataataa agacataaca tgctatttcg ctaaatcaaa gtttagcgaa ataaagggag
      121 gggcaaacag ggaaaatgag cagttatatc gatgtggtcc acccggaccg ccatgcgccg
      181 tatttggata tacccaatga tgtggtggaa tacctgcatt atctggactt tgtaaagatg
      241 cggtcaccgc gcaccatcaa cgcatactat ctcgatttgc gcggcttctt ccgttttatg
      301 atggcgatgc aatggtcgcg tgtgccgcgg gatacgccgc tggaagaaat aaacttaaag
      361 gatatctcaa ccgaggatat aaaaaagatc accaagcggg acatcttttc ctatttggac
      421 tatgtgcgca gcacggacgc caacggcggc aaagcccgtg cccgcaagct gagcgctcta
      481 aaaggctttt tctcctatct ggtgacccag gtgaataaac tgcaggaaaa tcccaccgag
      541 ggaatcagtc tcggctcacc aaaaaaggcc ctgcctaagt acctgactgc cgcggaaagc
      601 atagacttgt taaaaaatat acaaagtgac ttctatgagc gcgacttctg catgattacc
      661 ctgtttttaa actgcggcat gcggctgaca gaactggtga ccatcaacat gggcaatttc
      721 aagcaggata ccatccgcat caccggcaaa ggcaacaaag agcgactggt ctacctgaac
      781 gatgcctgct tattttcctt gcagcgctat aaaaaagccc gcgctgccct gccgaatctc
      841 gccgacaagg atgccttgtt cgtctccaaa cgcaccggca agcggctttc ggcgcgccgg
      901 gtcgagcaga tcgtcgcacg ttgcctgcaa agtgcaggtt tgagcgggcg cggattttcg
      961 ccccacaagc tgcgccacac cgccgccaca cttatgtatc agggcggcgt ggatatgctg
     1021 gctttaaagg aaattctggg tcacgaaaac gtctccacaa cgcagattta cacccacatc
     1081 aaccgcgacc agctacgcca ggcggtggcc tcttccccgc tggccgacaa aacgtttgaa
     1141 gaacagccgt cagcgcccga ccttggcagt gacgaaggcg aggaaagtcc cgacggcact
     1201 gaaaacagct gacagcacca gcgccgcgcc gaaccgtaca gccagccgcc gcagcgtgcc
     1261 aggcgtttcc cgcgtggcgc ggccgcatat aacattaaac agttcccctg ccaggcgtgc
     1321 cgaccagccc gccagccaca ggcaaaaaaa cagcgttgcc gcgtcggaaa cgattgttgc
     1381 taatcggtac tgcaccagcc ccgccgcacc gttcaccgct gccaccgagg ccgcgcagta
     1441 gcccaggaac acgccgcgcg ccgcaaacag cagcaccaac agacccacgc ccaacacaca
     1501 gaagccgcac aaaaatactg cagtcagctg taaaaagctc accgaaaatt ctgtcccgaa
     1561 aattgctgcg accgctgcat tttcgggact ttttgtgtaa tacgccgcca gctgctgccc
     1621 cagcgccgaa acaccgcccc gccccagcca gatgcccggc aggtagccag ccaggaacat
     1681 cgtgccaaac agccacaccg caccgctgtg cgccgttccc gcgcactctc ggcgcggtcg
     1741 ttctgcccgc cggggcagca gcaaggccgg gcggcgcagt gtcgtctgtg ccgcccgcat
     1801 cagcggctcc cccgcaaggc cgccagcgca ccgcccaaca gaccgcccag cagcagggcc
     1861 attggcagca tggcgtttgc cccctgccag tccagctcac cgctggctgc caatgtcgcc
     1921 gccatcaggc agaccgtata aaacgtacca cagcccagac cgcagagcag cagcttctgt
     1981 ttcagcctgc ccgccagtac ccgggccgat accgccgcac cgattgccgc cgccatacac
     2041 gccattgggc gcaccgccgc cagcggcaga cccgtgtggg tcagcaaaaa cgctcccagc
     2101 gccagcagca ccatacacgc ccccacgccg cagccaaagg caagcagcac tgccatcaca
     2161 gccggatttt ttgccgcagc tctgccttgt ttcggtcttt gcagattttt tccacccata
     2221 acaaaacgcc cccttgcaca gtcaacacta tgcaagaggg cattagtttt atgtcagatt
     2281 acttcttgac cggggtggat ttgctatcct tgccggcatc cagcttctcc acagaggaaa
     2341 ttgcagtgcg gcggaaacga atcttggtgc ggtcgctgcc agtctcgatg acgatggtgt
     2401 catccttatc agcgatggcg cagacgatgc cgacaatgcc gccaatggtg gtgaccttat
     2461 cgccgatctc aatagagctg cgcatggcgg cgtccttctt gtcctggcgc ttctgcggca
     2521 ggtagatcag aatgaccatg aaaacgataa tgagaatcat cggcaggaag ctgatgagca
     2581 tttcggcagt ggtagccgtg ccggtagcgg catccaggaa ctgaatcata gcaagtatac
     2641 tccttaaaaa taacgatttc ttctattata tcgaatatgt gcgcattgtg caagcgtttt
     2701 gcacgaaaaa tcagatgcgg gtgtcaagtt tatgcacata ggtgtcgtgg aactgctgga
     2761 aggtgccgtt gtccagcgcc tcacggatgc gctccatcag gtggttgtaa aaatacaggt
     2821 tgtgcataac ggccagacgc atccccagca actcgtccgc tttctgcaga tggcgaatgt
     2881 acgcgcggga gaagttgcgg cagaccgggc aatcgcagtg cgggtcaatg ggcgactcgt
     2941 cgcgctcgta tttcaggttc ttgatgttga tgatgccgtc ccaggtgaac aggtggccgt
     3001 ggcgggcgtt acggctgggc ataacgcagt cgaacaggtc aacaccgcgg tagacgccct
     3061 cgatgatgtt gcccggggtg ccgacgccca tcaggtagcg aatcttgttt ttcggcgcaa
     3121 acggttcaac agcagagata acatcgtaca tgacctcggc aggctcgccc accgccagac
     3181 ctccgatggc atagccgtcc agatcatact cagcaatctg cttcatatgc tcgacgcgca
     3241 gatcctcaaa cgtgcagccc tggttgatgc cgaacagcag ttgatccggg ttgacggcct
     3301 tttcctcgtg cttgaggcgg ttcatttcgt ccttgcagcg cagcagccag cgtgccgtgc
     3361 gggcacagct gttcttggcg tactcgtagg tggccgggtt ctcaacgcat tcgtcaaagg
     3421 ccatggcgat ggtggagccg aggttcgcct gaatctgcat gctctgctcc ggccccatga
     3481 aaatgcggtg gccgtcgagg tgggagttga aggtgacgcc ctcctcggtg atatgacgca
     3541 gcttggccag gctgaacacc tggaacccgc cgctgtcagt cagaatgggg ccgtcccact
     3601 tggtaaactt gtgtaggcct cccatctcgg ccacgagctt gtcacccggg cgcaggtgca
     3661 gatgataggt attgcacagc atgacctgtg cgcggatatc cttgagatcc agcgccgaca
     3721 ggccgccctt gatggcggcg gctgtggcga cgttctggaa cgccggggtc tgtaccgtac
     3781 cgtggaccgt ggtaaactca ccgcggcggg cggcaccttc ctgtttgata aggcggtaag
     3841 gcataggttt ctatctccgt ttttaaaatg attcatccga aaaaggcggc acagggggct
     3901 tttttccctg caccgcctaa tagattatta taaaagaaaa atcgtgattc gtcaaccgta
     3961 aaacggaagc ccaacgaccc tcgcacggaa ttattttacc atataacgca aaatggtttt
     4021 gagcttgctt ttttctgtgc ggttggcgtt attcagatag atttccctgt ggcagctttc
     4081 cgcacgtatc agactgagag cgcgtgtaaa tttgtccagc attgcaaaag acactggctc
     4141 atcatcatac gcgccaatgt gcaaaatctg cacgcaggtt ccgccctgca cgatttcaaa
     4201 gcggatttct tccaagagcg ggttcggctt tttgactttt gcgcattcca aag
//
LOCUS       DBMDSQ010000172         4250 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_4879_length_4250_cov_2.051097, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000172 DBMDSQ010000000
VERSION     DBMDSQ010000172.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 4250)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4250)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4250
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4879_length_4250_cov_2.051097"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..304)
                     /locus_tag="ACZPAX_06895"
     CDS             complement(<1..304)
                     /locus_tag="ACZPAX_06895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113663.1"
                     /translation="MPKIDIDNYIEQSGMRKARFGGYEPEDVRQAMLDLCADYEQHLA
                     AATAEARAARQENDALRRHAQNLMMQNQTLSGQNATLAGQVDKLQSYRANLETQFST"
     gene            complement(445..1443)
                     /locus_tag="ACZPAX_06900"
     CDS             complement(445..1443)
                     /locus_tag="ACZPAX_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005935035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGGtGRT protein"
                     /protein_id="HIR1113664.1"
                     /translation="MATFESQNRRMPKIEACLKANGLESLDACNEMLLAKGIDCDKII
                     RGIQPICFDNAVWAYTLGTAIAVKRGLKSAADCAAAIGEGLEAFTIPGSVAEQRKVGL
                     GHGNLGAMLLHEDTHCFAFLAGHESFAAAEGAIGIARTANKVRKEPLRVILNGLGKDA
                     AQIISRINGFTYVKTDYDFATGELKVVETIPYSDGERAAVKCYGANDVLEGVAIMKAE
                     GVEVSITGNSTNPTRFQHLVAGTYKKWAIENGKKYFSVASGGGTGRTLHPDNVAAGPA
                     SYGLTDSMGRMHGDAQFAGSSSVPAHVEMMGLIGAGNNPMVGMTVACAVAASQANA"
     gene            complement(1464..2165)
                     /locus_tag="ACZPAX_06905"
     CDS             complement(1464..2165)
                     /locus_tag="ACZPAX_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015537775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HIR1113665.1"
                     /translation="MEYSAQVNAMCPITKGPKHGPAPIPEEGQWVKAYKIEDISGYTH
                     GVGWCAPQQGTCKLSLNIKNGIIEEALVETIGCSGMTHSAAMAGEILPGKTILEALNT
                     DLVCDAINVAMRELFLQIVYGRSQTAFSENGLPVGAGLDDLGKGLRSMTGTIFSTKAK
                     GVRYLELTEGYILKTALDKDNQVIGYQFVRLGKMMEDIRHGKDPKEAYEKNIGTYGRF
                     SAEQGAVKYIDPREE"
     gene            complement(2486..4165)
                     /locus_tag="ACZPAX_06910"
     CDS             complement(2486..4165)
                     /locus_tag="ACZPAX_06910"
                     /inference="COORDINATES: protein motif:HMM:NF016800.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008754 - O-antigen ligase activity
                     [Evidence IEA];
                     GO_function: GO:0008755 - O antigen polymerase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HIR1113666.1"
                     /translation="MTQTQQLRRLAAQSATAFLVAALCAFPLYIDKFSNLGVVKFTGI
                     CTVCWAFALWLGALAVVGAKPMPDRLPWKTDPGLGALGAVTASGVLSTVLSLSPAASF
                     WGLGGYYGGCMMVLFTAAGYLAVRAFAPQKILNGLTFCVGVATAIVTVLYVLNIFNID
                     LIGTYADTAVVERAQFFSTLGQKNFCSGFMAFALPLVFYAFLVARGVRHTLFYGVPAF
                     FGGLALAVVDAEGLTLGIGAAVLVLICQKNFTTRTLRRLAVIGAFFFFHAGWMQYMRT
                     HVYTQGGKPMLAALGHVAQFGFTVCLAVWAVLRFALRGRKLPLYKLGRALAAVIVLGA
                     AVLTLLANFMPGFPSLGRLDDLLVFNDDWGTYRGTAWRITAESWLAQPVWRKLFGVGP
                     GMMHTAVAQWAGADITERMRTFYAAHNEYLELLLTTGVAGLAAWLWFVIAHLRKAAHN
                     WLRPGVAPVTLALVSYLAHAVISIRVSMIFPEIMLLFALLQVFCLPPEQEENSATTEK
                     PVRYGKGKKTKSAEPAEQPGLVRQWLPPIVAGIAMMAVCGAASRVIFGFLF"
BASE COUNT          867 a         1271 c         1309 g          803 t
ORIGIN      
        1 cagtggaaaa ctgggtttcc aggttggcgc ggtagctttg cagcttgtct acctgcccgg
       61 ccagcgttgc gttctggcca gaaagcgtct ggttctgcat catcaggttt tgggcgtggc
      121 ggcgcagggc atcgttctcc tgccgggcgg cgcgggcctc ggcggtagct gccgccaggt
      181 gctgctcata gtcggcgcag aggtccagca tggcctggcg cacatcctcc ggctcatagc
      241 cgccgaaccg cgccttgcgc ataccgctct gttctatgta gttgtcgatg tcaattttgg
      301 gcattggtag tttctcccaa gataaattta cacatactcg attttattat agaacatgat
      361 acgctgtaaa gcaagtaaaa cccacaaaaa agccgccctt tccttgcgga aagggcggcg
      421 aaaagcctaa tggttcagtt caaatcaagc gttggcctga gaagcagcaa cagcgcaggc
      481 gacggtcatg ccgaccatcg ggttgttgcc ggcgccgatc agacccatca tctcgacgtg
      541 agccgggacg gaggaagaac cagcgaactg ggcatcaccg tgcatacggc ccatggagtc
      601 ggtcaagccg taggaagcgg ggcctgcagc gacgttgtcc gggtgcaggg tacggcccgt
      661 gccgccgccg gaagcgacgg agaagtactt cttgccgttc tcgatggccc acttcttgta
      721 ggtaccggcg accagatgct ggaagcgggt ggggttggtg gagttaccgg tgatggaaac
      781 ctcaacgccc tcagccttca tgatggcaac gccttccaga acgtcgttgg cgccgtagca
      841 cttgacagca gcgcgctcgc cgtcggagta agggatggtc tcgacgacct tcagctcgcc
      901 ggtggcgaag tcgtagtcgg tcttgacgta ggtgaagccg ttgatacggc tgatgatctg
      961 ggcagcgtcc ttgcccaggc cgttcaggat gacgcgcagg ggctctttgc ggaccttgtt
     1021 ggcggtacgg gcaatgccga tagcgccctc agcggcggcg aaggactcgt ggccggccag
     1081 gaaagcgaag cagtgggtgt cctcatgcag cagcatggca cccaggttgc cgtggcccag
     1141 accgaccttg cgctgctcag cgacagagcc ggggatggtg aaagcctcga ggccctcgcc
     1201 gatggcggcg gcgcagtcag cagcgctctt caggccgcgc ttgacggcga tagcggtgcc
     1261 cagggtgtaa gcccagacgg cgttgtcgaa gcagatgggc tggatgccgc ggatgatctt
     1321 atcgcagtcg atgcccttgg ccagcagcat ttcgttgcag gcgtcaaggc tctccaggcc
     1381 gttggccttg aggcaagcct cgatcttggg catacggcga ttctgggatt caaaagttgc
     1441 cataatctat gttcctccct ctcttattct tcacgcgggt cgatgtactt gacagcgccc
     1501 tgctcggcag agaagcggcc gtaggtgccg atgttcttct cgtaggcttc cttcgggtcc
     1561 ttgccgtgac ggatgtcctc catcatcttg cccagacgga cgaactgata gccgatgacc
     1621 tggttgtcct tgtccagagc ggtcttgagg atgtagccct cggtcagctc cagataacgg
     1681 acgcccttgg ccttggtgga gaaaatggta ccggtcatgg aacgcaggcc cttgcccagg
     1741 tcgtccaggc cagcgccgac aggcaggccg ttctcagaga aagcggtctg gctgcggccg
     1801 tagacgatct gcaggaacag ctcacgcata gcaacgttga tagcgtcgca gacgaggtcg
     1861 gtgttcagag cctccaggat ggtcttgccg ggcaggatct cgccggccat agcggcggag
     1921 tgggtcatgc cggagcagcc gatggtctca acaagggctt cctcgatgat gccgttcttg
     1981 atgttcaggc tcagcttgca ggtgccctgc tggggagcac accagcccac accatgggtg
     2041 tagccgctga tatcctcaat tttatacgcc ttgacccact ggccctcttc ggggatgggc
     2101 gcgggaccgt gcttgggtcc ttttgtgatc gggcacattg cattgacctg tgcggagtat
     2161 tcgatcataa ttttctctcc tttgcatatt gttaggcttt tcacagctat ctttattata
     2221 aagggtcgaa tcctgttttt caagatgtta gtttaagcta acctttggtt ttttggcatt
     2281 ttctatgacc gggcggcatg gcaggcggcg gataggcatg gtggagcaaa taaaaaagcc
     2341 tcccttgtca aagggaggtg gacgccgcag gcggccggag ggattcctgc ccgcgtgcgg
     2401 atgtaacacc gtacaaggca aggcgcgtcg gaatccctcc gcctcgctat gctcggcacc
     2461 tccctttgac aagggaggct tttccttaaa acagaaaccc aaagatcacc cgcgaggctg
     2521 caccgcagac agccatcatc gcaatgcctg ccacaatcgg cggcagccat tgccgcacca
     2581 ggccgggctg ctccgccggc tcggcagatt tcgtcttttt gcctttgccg taccgtacag
     2641 gtttctccgt cgtggcggag ttttcctcct gctccggcgg cagacaaaac acctgcaaaa
     2701 gcgcaaacag cagcatgatc tcggggaaaa tcatcgacac gcgtatcgag atgaccgcat
     2761 gggccaggta gctgaccagt gccagtgtga ccggggccac gccggggcgc agccagttat
     2821 gggcggcctt gcgcaggtgg gcgatcacaa accacagcca cgcggccagc cccgccacgc
     2881 cggtggtcag cagcagttcc aggtactcgt tgtgggcggc gtaaaaggtg cgcatccgct
     2941 cggtgatgtc cgcgcccgcc cactgggcca cagccgtgtg catcatgccg gggccgacgc
     3001 cgaacagctt gcgccacaca ggctgcgcca gccagctttc ggcggtgatg cgccaggccg
     3061 tgccgcggta ggtgccccag tcatcgttaa aaaccagcag gtcatccagg cggcccaggc
     3121 tggggaagcc cggcataaaa ttggcaagca gcgtcagcac tgccgcaccg agcacaatga
     3181 ccgccgccag tgcccggccc agcttgtaca gcggcagttt ccgcccgcgc agggcaaacc
     3241 gcagcacggc ccagactgcc agacagaccg tgaacccgaa ctgcgccaca tgacccagcg
     3301 ccgccagcat tggcttaccg ccctgggtgt agacatgggt gcgcatatac tgcatccacc
     3361 cggcgtggaa gaagaaaaac gccccgatga ccgccagccg ccgcagcgtg cgggtggtga
     3421 agtttttctg gcagatgagc accagcaccg ccgcgccgat gcccaaagtc aggccctcgg
     3481 catccaccac agccagcgct aacccgccaa agaacgcagg cacgccgtaa aacagtgtgt
     3541 gccgcacgcc ccgcgcgact aagaacgcat agaacaccag cggcagcgcg aacgccatga
     3601 acccggagca gaagtttttc tgccccaatg tcgagaaaaa ctgcgcgcgc tccacgaccg
     3661 ccgtgtccgc ataggtaccg atgaggtcga tattaaatat gttgaggacg tacagcaccg
     3721 tgacgatggc ggtggcaacg ccgacgcaga aggtcagtcc gtttaaaatt ttctgcggcg
     3781 caaaggcgcg gacggccaga tagcccgccg ccgtgaacag caccatcata cagccgccgt
     3841 agtagccgcc caacccccag aaggacgccg caggcgacag cgacagcacc gtggacagca
     3901 cgcccgatgc cgtgaccgcc cccagtgccc cgaggccggg gtcggttttc cagggcaggc
     3961 gatccggcat cggcttggca ccaacgacgg ccagcgcacc gagccagagc gcaaacgccc
     4021 agcagaccgt gcagataccg gtgaatttga caacgccgag gttggagaat ttatcgatat
     4081 acagtgggaa cgcacagagc gccgccacca aaaatgccgt ggccgactgt gcagccaggc
     4141 ggcgaagctg ttgggtttgg gtcatagtga aaacctctta aagcacgtaa tttattgccg
     4201 gagctgctat gtaggggccg cacatgtgcg gcccgcaacg caacggaaat
//
LOCUS       DBMDSQ010000173         4240 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_4894_length_4240_cov_1.989128, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000173 DBMDSQ010000000
VERSION     DBMDSQ010000173.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 4240)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4240)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4240
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4894_length_4240_cov_1.989128"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..2150
                     /gene="ileS"
                     /locus_tag="ACZPAX_06915"
     CDS             <1..2150
                     /gene="ileS"
                     /locus_tag="ACZPAX_06915"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005941863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HIR1113667.1"
                     /translation="SIYVRFKLTDDPNGVLAKHNIPLDKAWIVIWTTTTWTLPANVAT
                     CLNPNLEYAFVKIGDAYHIMARELVESTMKGCHIDEYEVLPETVLGSELELMQYQHPF
                     LDRKGLVILGDHVTLEGGTGCVHTAPGHGVEDFEVCVNHYPQVPVVVPVDDAGRLTAE
                     AGEKFAGLKVWDANKVILEHIKESGHLMGVQHITHQYPHCWRCHHPIIFRATEQWFCS
                     IDAFKEDVYKAIDSVHWQPAWGHDRMAGMVRDRSDWCISRQRVWGVPIPVFYCKKCGK
                     YHITDASIKAVSDLFRKEGSDAWYKYDANDIMPKTEVCECGASDWEKDPDIMDVWFDS
                     GSTWSAVCRERPELHWPVDMYMEGADQFRGWFQSSLLTSVATQGVAPYREVLCHGWVV
                     DAQGKQMHKSAGNGMEPSEIIRDYGADIIRLWVASSDYTVDVRAGKEIFRQLSEAYRK
                     MRNTARFMLGNINDFDPAKDMVADDQLFEIDRWALDACNKLTANLRDAYDHYDFSRAY
                     HALYNFCVIDMSNFYMDVIKDRLYCADDHARRCAQTALYRILVDFTKLLAPILCFTSQ
                     EIWSYIPKLPGMKDYVVFEQMPEAKADADEAFEAKWARIMAIRDDVKKVLEQARADKT
                     IGSALEAGLTLYCSKEVYDFLNAIPMDELADLFIVSHVDLVEGEGGVKGLVEGLGVAA
                     AHAAGNKCLRCWKYEPSVGEDGLCPRCAKVLKQ"
     gene            2215..2712
                     /gene="lspA"
                     /locus_tag="ACZPAX_06920"
     CDS             2215..2712
                     /gene="lspA"
                     /locus_tag="ACZPAX_06920"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HIR1113668.1"
                     /translation="MIFGLFSVLGAAALVVIDQLIKHWATAALLPVGQMNVLPGIVEL
                     RYCLNDGMAFSMLAGKQGLLIGMTSVMLLVVLVMLFTRKMTAWERVAWTLVLGGGVGN
                     LIDRVLNGVVVDYINVLFMHFAIFNFADICVCVGVGLLMLVVLLDSCKKEKPAEQKAD
                     DDGAA"
     gene            2699..3631
                     /locus_tag="ACZPAX_06925"
     CDS             2699..3631
                     /locus_tag="ACZPAX_06925"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HIR1113669.1"
                     /translation="MEQLEFTAAADDTGRLDAWLAAQCPALSRSALQNLIEQGCVTKN
                     AAPVNKKEKVVPGAVYTVELPDPQPIEAQPQNIPLDIVYEDDDLLVVNKPKGMVVHPA
                     PGNPDGTLVNALLYHCAGQLSGIGGAIRPGIVHRIDKDTSGLLVVAKNDAAHQALSEQ
                     MSVHSIHRVYHAVAYGNLKDDEGFVEKWLGRDPRDRKKMAVLADNAVGAKYAYTGWQV
                     LERYGNFTYIACKLKTGRTHQIRVHMASTGHPLAGDAVYGPKNCIKSLNGQCLHAKEL
                     GFVHPRTGEWMQFDSPLPPYFADFITRLRKEHRA"
     gene            3628..>4240
                     /locus_tag="ACZPAX_06930"
     CDS             3628..>4240
                     /locus_tag="ACZPAX_06930"
                     /inference="COORDINATES: protein motif:HMM:NF019887.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HIR1113670.1"
                     /translation="MKPPLGDTLVICDMDNTLLTAKEGIPACNRAVIRLYTGMGGLFT
                     VATGRPPESIRAALGNLKLSLPAISCNGALLYDFSTESVLHRSTLNREQATTAIIDIL
                     AKFPRMGVEVMAGNGEMYVIHANEVTHAHQVDEHLGSIACPVENVPDGWVKVVFASDP
                     ESIRKLGQYAKTKYYGRDNYFLATNSIYLEIMPGGVSKASGLHD"
BASE COUNT          841 a         1294 c         1269 g          836 t
ORIGIN      
        1 attccatcta cgtccgcttc aagctgaccg acgaccccaa cggcgtgctg gctaagcaca
       61 acatcccgct ggacaaggca tggatcgtca tttggaccac caccacctgg acgctgcccg
      121 ccaacgttgc tacctgcctg aaccccaacc tggaatacgc cttcgtcaag atcggcgacg
      181 cttaccacat catggcccgc gagctggtcg agtccacgat gaagggctgc cacatcgacg
      241 agtacgaagt cctgcccgag actgttctcg gcagcgagct ggaactcatg cagtaccagc
      301 atccgttcct ggaccgcaag ggcctggtca tcctgggcga ccatgtcacc ctcgaaggcg
      361 gcaccggctg tgtccacacc gcccccggcc atggtgtcga ggactttgag gtctgcgtca
      421 accattaccc gcaggtgcct gtcgttgtcc ccgtggacga tgccggccgc ctgaccgccg
      481 aggctggcga gaagtttgcc ggtctgaagg tctgggacgc caacaaggtt atccttgagc
      541 atatcaagga gagcggccac ctgatgggcg tgcagcacat cacccaccag tacccgcatt
      601 gctggcgctg ccaccatccc atcatcttcc gcgccaccga gcagtggttc tgctccattg
      661 acgctttcaa agaggacgtc tacaaggcca tcgacagcgt gcattggcag ccggcctggg
      721 gtcatgaccg catggcgggc atggttcgcg accgcagtga ctggtgcatc agccgtcagc
      781 gcgtctgggg cgtgcccatc ccggtattct actgcaagaa gtgcggcaag taccacatca
      841 ccgatgcttc catcaaggct gtctccgacc tgttccgcaa ggagggcagc gacgcctggt
      901 acaagtatga tgccaacgac atcatgccca agaccgaggt ctgcgagtgc ggtgcttctg
      961 attgggagaa ggaccccgat atcatggacg tctggttcga ctccggctcc acctggagtg
     1021 ctgtctgccg tgagcgcccc gagctgcact ggcctgtcga tatgtatatg gagggcgccg
     1081 accagttccg cggctggttc cagtccagcc tgctgaccag cgttgccacc cagggcgttg
     1141 ccccgtaccg cgaggttctg tgccatggct gggtcgtcga tgcccagggc aagcagatgc
     1201 acaagtccgc aggcaacggc atggagcctt cggagattat ccgcgattac ggtgctgata
     1261 tcatccgtct gtgggtcgca tccagcgact acaccgtcga tgtccgcgcc ggcaaggaaa
     1321 tcttccgcca gctgtccgag gcgtaccgca agatgcgcaa caccgcccgc ttcatgctgg
     1381 gcaacatcaa cgactttgac cccgcgaagg atatggtcgc ggatgaccag ctgtttgaga
     1441 tcgaccgctg ggcgctggat gcctgcaaca agctgaccgc caacctgcgc gacgcctacg
     1501 accattacga tttcagccgt gcctaccatg cgctgtacaa cttctgtgtc attgatatgt
     1561 ccaacttcta catggacgtc atcaaggacc gcctgtactg cgctgacgac cacgcacgcc
     1621 gctgtgccca gaccgcgctg taccgcatcc tggtcgactt cacgaaactg ctggccccga
     1681 tcctctgctt taccagccag gagatttgga gctacatccc gaagctgccc ggcatgaagg
     1741 actacgttgt ctttgagcag atgcccgagg ccaaggccga tgccgacgag gcgtttgagg
     1801 ccaagtgggc ccgcatcatg gccatccgcg acgatgtcaa gaaggtcctg gagcaggccc
     1861 gcgctgacaa gaccatcggt tccgcgctgg aagccggcct gacgctgtac tgcagcaagg
     1921 aggtctacga cttcctgaac gccatcccga tggatgagct ggctgacctg ttcatcgtct
     1981 ctcacgttga cctcgtcgaa ggcgagggcg gcgtcaaggg cctggtcgaa ggtctcggcg
     2041 ttgcagctgc ccacgcggcc ggcaacaagt gcctgcgctg ctggaagtat gagccttccg
     2101 tcggtgagga cggtctgtgc ccgcgctgcg ccaaggtcct gaaacagtaa gttttactcc
     2161 gtcagcggtg cttatagtgg taagcaccgc tgattttttc ggataaggtg ttgtatgatt
     2221 ttcggtttgt tttctgtgct gggggcggcc gcgctggtcg tgattgacca gcttatcaag
     2281 cattgggcca cggcggcgct gctgccggtg ggtcagatga acgtgctgcc cggcattgtg
     2341 gagctgcgct actgtctgaa tgacggtatg gcgttctcaa tgttggcggg caagcagggc
     2401 ttgctcatcg gtatgacatc ggtcatgctg ctggtcgtgc tcgtcatgct gttcactcgc
     2461 aaaatgacgg cgtgggagcg cgttgcctgg acgctggtgc tcggcggcgg tgtgggcaac
     2521 ctcatcgacc gcgtgctcaa cggcgttgtg gtggattaca tcaatgtgct gttcatgcac
     2581 tttgccatct tcaactttgc ggatatctgc gtctgcgtgg gcgtcggcct gctgatgctg
     2641 gtcgtgctgc tggactcctg caagaaggaa aagcccgcag agcaaaaggc ggacgatgat
     2701 ggagcagctt gaatttaccg ccgcggccga cgataccggc cgcctggatg cctggctggc
     2761 cgcgcagtgc ccggcactgt cccgcagtgc gctgcaaaat ctcattgagc agggctgtgt
     2821 gacgaaaaac gccgcgccgg tcaacaagaa agaaaaggtc gtacccggtg cagtctacac
     2881 tgtggagctg cccgacccgc agcccatcga ggcccagccc cagaatatcc cgctggatat
     2941 cgtctatgag gacgacgacc tgcttgtcgt caataagccc aagggaatgg tcgttcaccc
     3001 ggctcccggc aacccggacg gcacgctggt caatgcgctg ctctaccatt gcgcgggaca
     3061 gctgtccggc attggcgggg ccatccgccc gggcatcgtc caccgtatcg ataaggacac
     3121 cagcggtctt ttggtcgttg ccaaaaacga tgcggcgcac caggccctta gcgagcagat
     3181 gagcgtacac agcatccacc gcgtctacca tgcggtggcc tacggcaacc tgaaagacga
     3241 tgagggcttt gtggaaaagt ggctgggacg cgacccgcgc gaccgcaaaa agatggccgt
     3301 gctggccgac aacgccgtcg gggccaagta tgcctacacg ggctggcagg tgctggaacg
     3361 ctacggcaac tttacctaca ttgcctgcaa gctcaaaacc ggccgcacgc accagattcg
     3421 cgtgcatatg gcatctacag ggcacccgct ggcgggggat gccgtttacg gcccgaaaaa
     3481 ctgcatcaaa tcgttgaacg gccaatgcct gcacgccaag gagttgggct ttgtgcaccc
     3541 cagaacaggg gagtggatgc agttcgattc tccgctgccg ccctattttg cggattttat
     3601 aacacggcta agaaaggaac atcgcgcatg aaaccacccc tcggcgatac ccttgtgatc
     3661 tgtgatatgg ataatacgct gctgaccgcc aaggagggca tcccggcctg caaccgtgcg
     3721 gttatccggc tgtacaccgg catgggtggg ctgttcacgg tcgccaccgg ccgcccgccg
     3781 gagtccatcc gcgcggcgct gggaaatctg aagctgtcgc tgccggctat ctcctgcaac
     3841 ggtgcgctgc tgtatgattt ttctacggaa tcggtgctgc accgctcgac attgaaccgg
     3901 gagcaggcca cgacggctat catcgatatc ctggcgaaat tcccgcgtat gggcgtggag
     3961 gtcatggcgg gcaacggcga aatgtacgtc atccacgcca acgaagtgac ccacgcccac
     4021 caggtggacg agcacctggg cagcatcgcc tgcccggtgg aaaacgtacc cgacggctgg
     4081 gtcaaggtcg tgtttgcctc tgaccccgag agcatccgca agctgggaca gtacgcaaag
     4141 acaaagtatt atggccgaga caactacttc cttgccacca acagtattta cttagagatt
     4201 atgcccggcg gcgtcagcaa ggccagcggc ctgcacgatt
//
LOCUS       DBMDSQ010000174         4213 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_4927_length_4213_cov_1.646158, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000174 DBMDSQ010000000
VERSION     DBMDSQ010000174.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 4213)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4213)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4213
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4927_length_4213_cov_1.646158"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1593
                     /locus_tag="ACZPAX_06935"
     CDS             <1..1593
                     /locus_tag="ACZPAX_06935"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HIR1113671.1"
                     /translation="GWCSVELYSLSFVLLVTVGLLLYYTVLRTRQWLCLLGLSLVFFA
                     ASGWQDFAFLLFTALTVWGGGLCLAKLDTDAAARRKQPGLPKEEKKAIKAQTTRRKKG
                     VLAGILVVNFLVLGYFKYWSHFAALLSGAWAASTPSALGLVMPLGISFYTFQAVGYLI
                     DCYRGRHPAEKNFARFLLFISFFPQLVQGPINRYGELEPQFMARHTWDWERTKRALFL
                     FAFGAMKKYAISNLTSPTIAAILDVEDASGLTGAMALLAILLYSLQQYADFSGGIDMV
                     LGVAQLYDIQMMPNFRQPYFSTSLGDFWRRWHISLGAWMRDYLFYPFALTKPMQRFGK
                     WAAKHGGKHLGRVLPAAVANLLVFAVVGIWHGPEIHYLVWGLYNGAVIALSDLLEPAF
                     KKLNAALHIPTESRAWHLFRILRTFVIVNIGWYFDRNGFMRGLLCLQKTFTDFRFDSL
                     AANAPGAFAAVSGPAWGIVIISTILVFVHSVLKENGRDPYADVQRLPLVVRWALYYLV
                     IFLTLISFICVTDTTGFLYANF"
     gene            1608..3134
                     /locus_tag="ACZPAX_06940"
     CDS             1608..3134
                     /locus_tag="ACZPAX_06940"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008723833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043038 - amino acid activation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HIR1113672.1"
                     /translation="MQTNILDWLEATAARCPDAPAVGDVQQDYTWAQLAELARRSGSA
                     LAARTAPRCPAAFYLEKSASAFAAMLGTVYAGCCYSVLDPRQPAARTRQVLDTLAPAL
                     LITDAEHADAAAKLSYSGPILPLESLLTADEDAALLAARRVQAVDVDPLYINFTSGST
                     GVPKGVTVSHRSVLDFIPQFAGIFGINGDDILGNQAPFDFDVSVKDLYTALYTGAKVQ
                     IIPRAYFSQPTKLMDYLADHAVTTLVWAVSAMCFVSIMNGFGYRVPSAVRQVLFSGEV
                     MPIKHLMKWKKALPAARFVNLYGPTEITCNCTYAILPDRDFAPDEVLPIGRAFPNEKV
                     FLLDESDALVTEPGQAGELCVSGTALALGYYADPVRTAQAFTQNPLNPNWYERIYRTG
                     DLARYDENGNLFYIGRKDFQIKHMGHRVELGEIEAATMRCDDVSRACCIFDAEKQRLH
                     LFYTGSCEKATLLTALKANLPPFMQPNTVQQLDELPTNKNGKIDRMALAALTKRGARK
                     "
     gene            3131..>4213
                     /locus_tag="ACZPAX_06945"
     CDS             3131..>4213
                     /locus_tag="ACZPAX_06945"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012500.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase family protein"
                     /protein_id="HIR1113673.1"
                     /translation="MTDAALQSIAAAYGTPTFVFDADALQARVRAIEEIFGPDIALCY
                     SIKANPFLLHAMSSVTARLEVCSPGELSICEQLHAADARVIYSGVNKTPVDIVRAVAD
                     GVGSYTAESLLQVRYLQEAALAAGKHLPVLLRLNAGSQFGMSKTDLFHALAHRAETPD
                     LEFIGIHYFVGTQRKKLANQQKELAMLRELYDEIEQTFGLRLSELEYGPGLSVPYFNG
                     DDFTDTLAPAKTLAPALREAARWAHLTVEMGRFYTAECGYYLTTAMDCKDHDGQHYCI
                     VDGGMNHLNYLGQIMGMKRPHLRHFAAQAGETLDWTLCGSLCTTNDVLVRSMPLDGLA
                     PGDLLVFENAGAYSVTEGLGLFLSRDL"
BASE COUNT          756 a         1340 c         1195 g          922 t
ORIGIN      
        1 ggatggtgta gtgtggaact ctattcccta agctttgttc tgctggttac ggttggcctt
       61 ttgctgtatt ataccgtgct gcgcacccgc caatggctct gcctgctggg gttgagtctg
      121 gtgttttttg cagccagcgg ctggcaggac tttgcgtttt tgctttttac ggccctgacg
      181 gtctggggcg gtgggctttg ccttgccaag ctggacaccg atgccgccgc ccgccgcaaa
      241 cagcccggct tgccaaaaga agaaaagaag gccatcaagg cccagaccac ccgccgcaaa
      301 aagggcgtgc tggccggtat cctggttgtg aactttctgg tgctgggcta ctttaaatat
      361 tggtcccact ttgcggcact gctttccggg gcttgggccg ccagcacgcc gtcggcgctg
      421 gggctggtca tgccgctggg catcagcttt tatacatttc aggctgtggg ctacctcatc
      481 gactgctacc gcggccgcca cccggctgag aaaaactttg cgcgttttct cttgttcatc
      541 agctttttcc cgcagctcgt gcaggggccg atcaaccgct acggcgagct ggaaccccag
      601 ttcatggccc gtcatacctg ggattgggag cgcaccaaac gtgcgctgtt cctgttcgcc
      661 ttcggcgcga tgaagaaata cgcaatttcc aacctgacgt ccccgacgat tgccgcgatt
      721 ttggacgttg aggatgccag cggccttacc ggcgcgatgg cgctgctggc aattttgctg
      781 tattctttgc agcagtacgc tgatttttcc ggcggtatcg atatggtgct gggcgtcgcg
      841 cagctgtatg acatccagat gatgcccaac ttccgccagc cgtatttttc cacctcactg
      901 ggggatttct ggcggcgttg gcatatctcc ctcggcgcat ggatgcgcga ctacctcttt
      961 taccccttcg ccctgaccaa gccgatgcag cgtttcggca aatgggcagc caagcacggc
     1021 ggcaaacatt tgggccgcgt actgcccgct gctgtggcga acctgctggt gttcgccgtc
     1081 gtcggcatct ggcacggccc ggagattcac tacctcgtct ggggtctcta taacggcgcg
     1141 gtcattgctc tgtctgactt gctggaaccc gcattcaaaa agctcaacgc tgcgctgcat
     1201 atccccactg agagccgcgc ctggcatctg tttcgcattc tgcgcacctt tgtcatcgtc
     1261 aacatcggct ggtactttga ccgcaacggt tttatgcgcg ggctgctctg cctgcaaaag
     1321 acctttacgg atttccgctt tgactcgctg gcggcaaacg ctcccggcgc atttgccgct
     1381 gtttcggggc ctgcgtgggg catcgtaatc atcagcacca tactggtctt tgtacatagt
     1441 gttttgaagg aaaacggccg tgacccctac gcggatgtgc agcgcctgcc gctggtcgtg
     1501 cgctgggcgc tgtactatct cgtcatcttc ctgacgctaa tcagctttat ctgcgttacc
     1561 gacaccaccg gcttccttta cgcgaatttc tgagaaaaga gttttttatg caaaccaata
     1621 ttctggattg gcttgaagcc accgccgccc gctgccccga tgcccccgcc gtgggggatg
     1681 tgcagcagga ctacacctgg gcgcagctgg cagaattggc ccgccgcagc ggcagtgccc
     1741 tggcggcccg caccgcgccc cgctgcccgg cagcgttcta tctcgaaaaa tctgcctcgg
     1801 cctttgccgc tatgctgggc acggtctatg cggggtgctg ctactcggtg ctggaccccc
     1861 gccagcccgc cgcccgcacc cggcaggtgt tggatacact tgccccggca ttgctcatca
     1921 ccgacgccga acacgccgac gctgcagcaa agcttagcta ttccggtccg attcttccgc
     1981 tggaaagtct gctgaccgcc gatgaggacg ccgcactgct ggctgcccgc cgtgtccagg
     2041 ctgtggacgt tgacccctta tacatcaact ttaccagcgg cagtaccggc gtgcccaagg
     2101 gcgtcacggt cagccaccgc tcggtgctgg atttcatccc gcagtttgcc ggtatttttg
     2161 gcatcaacgg ggacgatatc ctcggcaacc aggccccctt tgattttgac gtctcggtca
     2221 aggatctgta caccgccctt tacaccgggg ccaaggtgca gatcatcccc cgggcctatt
     2281 tcagccagcc taccaagctg atggactacc tggccgacca cgctgtcacc acgctggtct
     2341 gggctgtcag cgccatgtgc tttgtctcca tcatgaacgg cttcggctac cgcgtgcctt
     2401 ccgccgtgcg ccaggttctt ttcagcggcg aggtcatgcc catcaagcac ctgatgaagt
     2461 ggaagaaagc gctgcccgct gcccggttcg tcaacctcta cggccctacg gaaatcacct
     2521 gcaactgtac ctacgccatc ctgcctgacc gggattttgc ccccgacgag gtgcttccca
     2581 ttggccgcgc cttcccgaac gagaaggtct ttctgctgga cgaaagcgac gcccttgtga
     2641 ccgaaccagg ccaggcaggg gagctttgtg tctccggcac ggcactggcg ctgggctatt
     2701 acgctgaccc ggtgcgcaca gcccaagcct ttacccagaa tccgctgaat cccaactggt
     2761 acgagcgcat ttaccgcacc ggcgatctgg cgcggtatga tgaaaacggc aatttattct
     2821 acatcggccg caaggatttc caaatcaagc atatgggcca ccgcgtcgag ctgggcgaga
     2881 tcgaagccgc caccatgcgg tgtgacgacg tgagccgtgc ctgctgcatc tttgacgccg
     2941 aaaagcagcg actgcacctg ttctacaccg gcagctgtga gaaagccacc ctgctgaccg
     3001 cgctgaaagc caacctgccg ccctttatgc agcccaacac ggttcagcag ctggacgaac
     3061 ttcccacgaa caaaaacggg aagatcgacc gaatggcgct ggccgccctg acgaaaagag
     3121 gagcacgcaa atgactgacg ccgctttaca aagcattgcc gctgcctacg gcacgccgac
     3181 cttcgtcttt gatgccgacg ccctgcaagc ccgcgtgcgg gccatcgagg agattttcgg
     3241 cccagacatt gcgctgtgct attccatcaa ggccaatccg tttttgctgc acgctatgtc
     3301 ctccgtcacc gcgcggctgg aagtgtgcag ccccggcgag ctttccatct gcgagcagct
     3361 tcatgccgcc gacgcccgcg tcatctactc tggcgtgaac aaaacgccgg tggatatcgt
     3421 ccgcgccgtg gcagacggtg tcggcagcta cacggcggaa tcccttttgc aggtgcgcta
     3481 tctgcaagag gccgccctcg cggcgggcaa gcacctgccg gtgctgttgc ggctgaatgc
     3541 gggcagtcag ttcggtatgt ccaaaacgga tttgttccac gcgctggcgc atcgcgccga
     3601 aacgcccgat ttagagttca tcggcatcca ctattttgtg ggcacccagc gcaaaaagct
     3661 ggcgaatcag caaaaggaac tggcgatgct gcgcgagctt tatgacgaga tcgagcagac
     3721 ctttggcctg cgcctgtcag agttggaata cggccccggc ctgtcggtgc cttattttaa
     3781 cggcgacgat ttcaccgaca ccctcgcccc ggccaaaacg ctggcccccg ccttgcggga
     3841 agccgcccgc tgggcgcacc tgaccgtgga gatgggccgc ttttacaccg ccgaatgcgg
     3901 ctattacctg actactgcca tggactgcaa ggaccacgac ggccagcact actgcattgt
     3961 ggacggcggc atgaaccacc tgaactacct gggccaaatc atgggcatga agcgcccgca
     4021 cctgcgccac tttgccgccc aggctgggga gacactggat tggaccctct gcggcagcct
     4081 gtgtaccacc aacgatgtac tggtgcgcag tatgccgttg gacggccttg cccccggcga
     4141 cctgcttgtc tttgagaatg ccggggctta ctccgtcacc gaggggttgg ggctgtttct
     4201 cagccgcgac ctg
//
LOCUS       DBMDSQ010000175         4211 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_4929_length_4211_cov_1.452311, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000175 DBMDSQ010000000
VERSION     DBMDSQ010000175.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 4211)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4211)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4211
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4929_length_4211_cov_1.452311"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1662
                     /locus_tag="ACZPAX_06950"
     CDS             <1..1662
                     /locus_tag="ACZPAX_06950"
                     /inference="COORDINATES: protein motif:HMM:NF024863.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HIR1113674.1"
                     /translation="ERYEEKAGMSPTQRKACVVLALCALAIVLSIVVAWVLPGKLGLF
                     NGGASAYDKDAYPLDTSLEAILPQAAADDAYITASVFAGDQYAVTLQKDARITLNQFA
                     GQEGLQTAKALSTACVNFASDSNNYTIPQAIPKMKARRVFVQLGSNDVDGTVSVDSFI
                     ADYKQFLQNIHSAYSYADIIAVSIPPVTEDSANAAETQTYIDQFNQAIAVACSDMGFK
                     YLNSAETLKNSRGFAENSYMEANGYSASGARALLEYAKSHAYNTMDSRPDTSDIPQRA
                     SSTSGAASTPLPTATPTKFTASYEVEDSAKGTLTGNGQTGVSSVEIQADSGTSVNVTA
                     VAADGFVFYKWSDGITTATRYDNVTKDISVKAMFNDARVELTLDKGDTTIKKGESLTV
                     NATVKLGGKAYDATNVQWSINDEMEKNGSSLTFTPDAAGTYTLKAGIEINGTFSTAQL
                     TVTVEADAATVNISGNSTLTAGQSTTLTATVANGSGDTVWGCEETSWKATGDQVTFTA
                     NEPGTYRIHAANNGTESVFILTVTAAATPEPTATPAPAPESQAES"
     gene            1685..2440
                     /locus_tag="ACZPAX_06955"
     CDS             1685..2440
                     /locus_tag="ACZPAX_06955"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005924005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HIR1113675.1"
                     /translation="MAIYTCVLLDIDNTLLDFDAAERQAVTDMLAEYELPHDEAAYET
                     YHKVNRELWDSLAKGQLNKQKLFQIRFSRFMQAMQLPDNGKGKAMNDRYEELLATHAD
                     LLPGALTALEELSEVATLAIVSNGAAAVQESRIAASGIDRYMDGIYISEKVGVAKPSA
                     KLFEHAFHDLGITNKSRVLMVGDDLLADIKGGMNAGVDTCWFNPGNVENKTGITPKFT
                     VGSYEELYRIVMEPEELENLGVRNRRHSNEALL"
     gene            2510..>4211
                     /locus_tag="ACZPAX_06960"
     CDS             2510..>4211
                     /locus_tag="ACZPAX_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005924008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HIR1113676.1"
                     /translation="MLLELQNLGKSFGEHEVLRDVNAGVERGDRIGIIGANGTGKTTL
                     LRILCGESLPDAGDAAFGTGVTTGYLEQNARLDPALDVYATMKLVFTPALDAMQQMET
                     LQKQLAADPHNAELTEKIAHCTAVIDAMDAYNMDTQIKKVLNGMGFPADTWAKLAGVL
                     SGGEQTRLRLARLLLERPDLLILDEPTNHLDLETMEWLENYLKAYRGAVLVVTHDRYF
                     LDAVCTRIWELRGKSIKTYRGNYSAYLPQREAADERQQKLHDAAVEKAAKLQDYVDRN
                     LVRASTTKMAQSRRKQLEKLEIVEAPTAEAWELNFRFEYDIEPYDELVIMKNLSVHIG
                     ERTLIDALDYVVHRGDKLVIAGPNGAGKSTLLQVLDGKRRPSGGMVRLGSGAKPGIFT
                     QQQARRAGRVIDAIWNQYPRFTELEVRSHLARFGYRGEEVFKDCASLSGGELARLRFA
                     ELALERPNLMFLDEPTNHLDIYMRESLTQALAAYTGTLLLVTHDRYLMQTLGCPIMYL
                     EDGKATFYPNFAALQNREAGKTQEQQKTEDTAKKQAYGKEQRKRRAEVRARLKKVETE
                     I"
BASE COUNT          954 a         1322 c         1238 g          697 t
ORIGIN      
        1 gaacgttatg aggaaaaagc aggtatgagc cccacccagc gcaaggcgtg tgtggtgctg
       61 gcgctgtgtg cgctggctat cgtgctgagc attgtggtcg cctgggtact gcccggcaag
      121 ctgggccttt ttaacggcgg tgcaagcgcc tatgataagg acgcctaccc gctggataca
      181 tcgctggagg ctatactgcc ccaggctgcg gcggatgatg cctatatcac cgccagtgtc
      241 tttgcgggcg atcagtatgc tgtcacgctg caaaaggacg cccgcatcac gctgaaccag
      301 tttgcggggc aggaggggct gcaaaccgcc aaggcgctgt ccacggcctg cgtaaacttt
      361 gcctcagact ccaacaacta cacgatcccg caggcaatcc ccaagatgaa agcccgccgt
      421 gtttttgtgc agctgggctc caacgatgtg gacggaacgg tcagtgtgga ttccttcatt
      481 gccgattaca agcagttctt gcagaacatc cactcggcct actcctatgc ggatatcatc
      541 gcggtgtcca tcccgcctgt caccgaggac agtgccaacg ccgccgagac ccagacttac
      601 attgatcagt tcaaccaggc cattgccgtg gcctgcagcg acatgggctt caaatacctg
      661 aacagcgccg agaccctgaa aaacagccga ggctttgccg agaacagcta catggaagcc
      721 aatgggtaca gcgcctccgg tgcccgcgcc ctgctggaat acgccaagtc ccacgcctat
      781 aacacgatgg acagccgccc ggacaccagc gacatcccac agcgcgcctc gtccaccagc
      841 ggcgcggcca gcacgccgct ccccaccgcc accccgacga agttcactgc cagctatgag
      901 gtggaggaca gcgccaaggg caccctgacc ggcaacggcc aaaccggcgt ttccagcgtg
      961 gagatccagg cggactccgg caccagtgtc aacgtgacgg cggtagccgc cgacggcttt
     1021 gtattctaca agtggagcga cggcatcacc accgcgaccc gctatgacaa cgtgacgaag
     1081 gacatctcgg tcaaggccat gttcaacgac gcccgtgtcg aactgacgct ggacaagggc
     1141 gacacgacga tcaagaaggg cgagagcctg accgtcaacg ccaccgtcaa gctgggcggt
     1201 aaggcctacg acgccaccaa tgtgcagtgg tccatcaatg acgagatgga gaaaaacggc
     1261 agcagcctga cctttacgcc cgacgctgcc ggaacctaca ccctcaaagc gggcatcgag
     1321 atcaacggga ccttctccac cgcgcagctg accgtgactg tggaggccga cgccgcgacg
     1381 gtgaacatca gcggcaacag caccctgacc gcgggccaga gcacgaccct gactgcgacc
     1441 gtcgccaacg gcagcggcga tacagtttgg ggctgcgagg aaacgtcctg gaaagccacc
     1501 ggcgaccagg tgacattcac cgccaacgag ccgggcactt accgcatcca cgccgccaac
     1561 aacggcaccg agagcgtctt tatcctgacg gtcacggctg ccgccacgcc ggagccgacc
     1621 gccacaccgg cccctgcgcc tgagagccag gcggaatcct aaaacgcgac agaggttaca
     1681 agaaatggca atttacacct gtgtcctgct ggacattgac aatacgctgc tggactttga
     1741 cgccgccgag cgccaggctg tgaccgatat gctggccgag tacgagctgc cccacgacga
     1801 ggccgcctac gagacctacc acaaggtcaa ccgtgagctg tgggactcgc tggcaaaggg
     1861 ccagctcaac aagcaaaaac tgttccagat tcgtttttcc cgctttatgc aggccatgca
     1921 gctgccggat aacggcaaag gcaaggccat gaacgaccgc tatgaagaac tgttggcgac
     1981 ccacgccgat cttctgcccg gtgccctgac cgcgctggaa gaactcagcg aggtggcaac
     2041 gctggccatc gtctccaacg gcgctgccgc cgtgcaggag tcacgcattg cagcctccgg
     2101 cattgaccgc tatatggacg gcatttatat ttcggagaag gtaggcgtgg ccaagcccag
     2161 cgccaagctg tttgagcacg ccttccacga tttgggcatc acgaacaaaa gccgcgtgct
     2221 gatggtcggc gatgacctgc tggccgatat caaaggcggc atgaacgccg gggtggacac
     2281 ctgctggttc aaccccggga atgtggaaaa caagaccggc atcacgccaa aatttacggt
     2341 cggttcctat gaggaactgt atcgcattgt gatggagccg gaggagctgg aaaacctggg
     2401 cgtgcgcaac cgccgtcaca gcaacgaagc cctgctgtaa aaaccggggc acactgccgc
     2461 tgcccgcaaa aggcggcggc atcattttgg aattgaggat gtaccgtata tgttgttgga
     2521 actgcaaaac ctgggcaaga gcttcggcga acacgaggta ctgcgggatg tgaacgcggg
     2581 cgtcgagcgc ggcgaccgca tcggcattat tggtgcaaac ggcaccggca aaacgacact
     2641 tttgcgtatt ctgtgcggcg agagtctgcc cgacgcgggc gacgccgcct tcggcaccgg
     2701 cgtgacgacc ggatatctgg aacagaacgc ccggctcgac ccagcactgg atgtctacgc
     2761 cacgatgaaa ctggtgttca ctccggcgct ggacgcgatg cagcagatgg aaaccctgca
     2821 aaaacagctg gctgccgacc cgcacaacgc tgagctgacc gaaaagattg cccactgcac
     2881 cgctgttatc gacgccatgg atgcctataa catggacacc caaatcaaaa aggtccttaa
     2941 cggcatgggc tttcccgcgg acacctgggc gaagctcgcg ggcgtgcttt ccggcggtga
     3001 gcagacccgc ttgcgcctgg cgcggctgct gctggaacgc cctgacctgc tgattctgga
     3061 cgagccgacg aaccacctgg acctggaaac gatggagtgg ctggaaaatt acctgaaagc
     3121 ctaccgcggt gcggtgctgg tcgtcaccca tgaccgctat ttccttgacg ctgtctgcac
     3181 ccgtatctgg gagcttcgcg gcaaaagcat caagacctac cgcggaaact actcagccta
     3241 cctgccccag cgcgaagccg ctgacgagcg ccagcaaaaa ctgcatgacg ccgccgtgga
     3301 aaaggctgcc aaattgcagg actatgtgga ccgcaacttg gtccgtgcct ccaccaccaa
     3361 gatggcccag agccgccgca agcagctgga gaaactggag atcgtcgagg cacccaccgc
     3421 cgaggcgtgg gaactgaact tccgctttga gtacgacatt gagccgtatg acgagttggt
     3481 catcatgaaa aacttgagcg tacacatcgg cgagcgcacc ctcatcgatg cactggatta
     3541 cgttgtccac cgcggagaca agctcgtcat tgccggcccc aacggcgcgg gcaaatcgac
     3601 gcttttgcag gtgctggacg gcaagcgccg cccttctggc ggcatggtgc gtttgggcag
     3661 cggggccaaa cccggcatct tcacccagca gcaggcccgc cgggcgggcc gcgtcatcga
     3721 cgccatctgg aaccagtacc cccgcttcac cgagctggaa gtccgcagcc atctggcacg
     3781 cttcggctac cggggcgagg aagtctttaa ggactgcgcg tccctctccg gcggcgagct
     3841 ggcccgcctg cgctttgctg agctggccct tgagcgcccg aacctgatgt ttttggacga
     3901 gccgacgaac cacttggata tctacatgcg cgagagcctg acccaggcgt tggccgccta
     3961 caccggcacg ctgctgctcg tcacccacga tcgctatctg atgcagacgc tgggctgccc
     4021 catcatgtat ttagaggacg gtaaggcgac gttttacccg aattttgcag cgctgcaaaa
     4081 ccgcgaagcg ggcaaaaccc aagagcagca gaaaaccgag gacactgcca aaaagcaggc
     4141 ctacggcaag gaacagcgca agcgtcgcgc cgaggttcgc gcccgcttaa agaaagtgga
     4201 aacagagatc g
//
LOCUS       DBMDSQ010000176         4170 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_4982_length_4170_cov_1.930204, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000176 DBMDSQ010000000
VERSION     DBMDSQ010000176.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 4170)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4170)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4170
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4982_length_4170_cov_1.930204"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..363)
                     /locus_tag="ACZPAX_06965"
     CDS             complement(<1..363)
                     /locus_tag="ACZPAX_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005931246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR1113677.1"
                     /translation="MQTVIETKSLCKQYGTRMAVDHVELHVPQGCVYGFIGPNGAGKS
                     TTMKMLLGLIHPTSGSVRLLGQPLTRESRLALLRQTGSLIENPAGYLHLTAQENLEIV
                     ADLKGVPHKDIGRVLDIVH"
     assembly_gap    636..645
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            647..1288
                     /locus_tag="ACZPAX_06970"
     CDS             647..1288
                     /locus_tag="ACZPAX_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR1113678.1"
                     /translation="MADNLHTAGYKTVRTAASCADAKTAFARRRPELMILDINLPDGD
                     GFGLFRDLHAGSDVPALFLSARDADADRLFGLGLGADDYLTKPFLMQELLLRVQHILQ
                     RAYRAELCRARTVALGSVTVDLQDAAVHRAEGTAALTATERAILQKLADERGHIVTYD
                     ALCEAVWGADYYGYENSLNVHIRHLREKIEADPGRSQWLVTARGIGYKLAGEM"
     gene            1285..2667
                     /locus_tag="ACZPAX_06975"
     CDS             1285..2667
                     /locus_tag="ACZPAX_06975"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005931240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR1113679.1"
                     /translation="MKLKTFTRMIRRYVLATAAVVLLVVGLLLGLTVYLGLHYGETSD
                     DRARIGTLAEALTPTADGLQFDPAQTPAQWMQGYAWAMVLDGDGTVIWQYDLPQNLNK
                     HYTASEIASFSRWYLDDWPVFCWTADYGLFVAAQPRGSVWKYNIYSSAALMYATAAGL
                     LPTLGVLLAAVVGCCVFFSWRGARQLRAIAAGLDTIADGGTVELPTTGYAGELADAVN
                     RASEQLRRRKEIIARRDDARTNWIAGVSHDVRTPLALILGWAEQLEQDAALPAAVRQK
                     AGDIRTQSEKLRALIEDLNLTSKLQYGAQPLRRQPVQAGPLLRRLAAEFCESPLAAGC
                     DMAVELPPDADKAVLHADAALLGRAVENLLNNAARHNAPGVHITLAAVLRENALELTV
                     ADDGAGYPAAVLHALQTGETGANTPHILGLHVVEQIAQAHGGSVQFMQNAPQGAKAVV
                     TLPAENQAHR"
     gene            3041..>4170
                     /gene="ispF"
                     /locus_tag="ACZPAX_06980"
     CDS             3041..>4170
                     /gene="ispF"
                     /locus_tag="ACZPAX_06980"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005940105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HIR1113680.1"
                     /translation="MENQNLPAVSAIIVAAGASRRMGFDKLSFRLPDGRTVLETSCAA
                     LAAHPAVTQLVLVCGGNRAACEAIAAACPKPCAVVQGGATRADSVRNGLAAATGELVA
                     IHDAARPFISAEVITAALTAAAASGAAAPAVPVKDTIKIAGKSGRVVETPDRAALYAV
                     QTPQCFQRTLYLQALDAVTGEKASLVTDDCSLFELAGLPVTLTAGDYANYKITTKEDL
                     QKEKTMRIGHGYDVHRLVEDRKLILGGVEVPYEKGLLGHSDADVLLHAVMDAVLGAAA
                     LGDIGQHFPDTDPAYKGADSLALTREVAKIIAQHGYKVGNIDATILCQRPKLAPHIPA
                     MRQNIADAFGLPLDAVSVKATTEEHLGFTGEGLGIAAHAVAL"
BASE COUNT          771 a         1323 c         1315 g          751 t
ORIGIN      
        1 gtggacgata tccagcacgc ggccaatgtc tttgtggggc acgcccttta agtcggcgac
       61 gatttccagg ttttcctgcg cggtcaggtg caggtagcca gccgggtttt caatcaggct
      121 gccggtctgg cgcagcagag ccaggcggct ttcgcgggtc agcggctggc ccagcagccg
      181 cacgctgccg gacgtcgggt ggatcagccc cagcagcatc ttcattgtcg tggatttgcc
      241 cgcaccgttt ggcccgatga acccatacac gcagccctgg ggcacgtgca gctccacatg
      301 gtcaacggcc atccgcgtgc cgtactgctt gcacagagat tttgtctcaa ttaccgtttg
      361 cattgtcaac gctccttttt gtttcggtgg tttcagcata cactgccgac cttaaaggag
      421 ctgtaagggc agcttaattt tggcttaaat ttaggggcgt gttatggtgg agctgattga
      481 atcgttgtat gataggtagg ggccgggcat acccagcccg cagccgcatg gcagatgacc
      541 gtttcaggaa aaaccgtggg ccgcctatgt gcggccccta cattctcaac accaatgtaa
      601 agggatgcaa ccatgaccac catttacgac gttgannnnn nnnnntgtgg cggataacct
      661 ccacacggcg ggctacaaaa ccgtgcgcac cgccgccagc tgtgccgatg ccaaaaccgc
      721 ctttgcccgc cgccgccccg aattgatgat tttggacatc aacctgcccg acggcgacgg
      781 cttcgggctg ttccgcgacc tgcacgcagg atccgatgtg ccggcgctgt ttctctcggc
      841 ccgggatgcc gacgccgatc ggctgttcgg cttaggcctc ggcgcggacg actacctgac
      901 aaaaccgttt ctgatgcagg aactgctcct gcgcgtccag catattttgc agcgcgccta
      961 ccgggccgaa ctgtgccgcg cccgcacagt agcgctgggc agtgtcacgg tggacctgca
     1021 ggacgccgcc gttcaccgcg cggagggcac tgccgcgctg acggccaccg agcgggccat
     1081 cctgcaaaag ctggccgatg agcgcgggca tatcgtgacc tacgacgccc tgtgtgaggc
     1141 cgtctggggc gcggactact acggctatga gaacagcctg aacgtacaca tccgccattt
     1201 gcgggagaaa atcgaggccg atcccggccg ttcgcagtgg cttgtgaccg cccggggcat
     1261 cggctacaaa ctggcagggg agatgtgaag ttaaaaacct ttacacgcat gatacgccgc
     1321 tatgtgctgg ccacggcagc ggtggtgttg ctggtggtgg gcctgctgct ggggctgacg
     1381 gtctatctgg gcctgcatta tggcgaaaca tccgacgacc gtgcccgcat cggcacactg
     1441 gccgaggccc tgacccccac cgccgacggc ctgcaatttg accccgccca gacgcctgcc
     1501 caatggatgc agggctacgc ctgggccatg gtgctggatg gggacggcac ggtcatctgg
     1561 cagtacgacc tgccgcaaaa tttaaataaa cactacacag cgtcggaaat cgcgtccttc
     1621 tcccgctggt atctcgacga ttggccggtg ttctgctgga cggcggacta cggtttgttt
     1681 gtggcggcgc agccccgcgg ctcagtctgg aagtacaaca tttacagcag tgccgccctg
     1741 atgtacgcta cggcagcggg cctgctgcct acgctggggg tgctgctggc cgctgtggtg
     1801 gggtgctgcg tctttttcag ctggcggggg gcgcggcagc tgcgggccat tgcggcaggg
     1861 ctggacacca ttgccgacgg cggcacggtg gaactaccca ccaccggcta tgcgggcgag
     1921 ctggccgacg cggtcaaccg cgccagtgag cagctgcgcc gccgcaagga aatcattgcc
     1981 cggcgggacg acgcgcggac gaactggatc gccggggtca gccatgatgt gcgcacaccg
     2041 ctggcgctga ttctgggctg ggccgaacag ctggaacagg acgccgcgct gcccgccgcc
     2101 gtgcggcaga aggccgggga catccgcacc cagagcgaga agctgcgggc gctcatcgag
     2161 gatttgaacc tgaccagcaa actgcaatac ggcgcacagc cgctgcgccg ccagcctgtg
     2221 caggccgggc ccttgctgcg ccgcctggcc gccgagttct gcgaaagccc gctggcggcg
     2281 ggctgcgaca tggcggtgga actgccgccc gatgctgaca aggctgtgtt gcacgccgat
     2341 gccgccctgc tgggccgcgc ggtggaaaat ctcttgaaca acgctgcacg ccacaacgcc
     2401 cccggcgtgc atatcacgct ggcggcggtc ctgcgcgaaa acgcgctgga actgacagtg
     2461 gccgacgatg gcgcgggcta cccggcagcg gtgctgcacg ccttgcagac cggtgaaaca
     2521 ggggcgaata cgccccacat cctgggcctg cacgttgtcg agcagatcgc ccaggcccac
     2581 ggcggcagtg tgcagtttat gcagaacgca ccgcaggggg caaaggccgt ggtgacattg
     2641 cccgcagaaa atcaggcaca taggtagggg cgggacgtgt cccgccctta gggcaatacc
     2701 gcataattct aagtgccgat acggcgagcg aggtgcggca gctgctaagc caaaagcgca
     2761 gataatactt tgtgtattat cgagcatttt ggcaacgcag atgccgtgcc gcagccgccg
     2821 gagcggtgct taagccgtaa ggcgggaatt gtgcggtatt gccttagctc tctcgaaata
     2881 tcgcgcttgc cgtacggtgg agggccgggc ctgcccggcc cctacgggaa atgcgatact
     2941 tttttcactc cgcacaaaaa atcatccaga ttttagtgga ttttttctgt atgaccttgt
     3001 ataatattta ttatacctat ataaaggggg cactttgccc atggaaaacc aaaacctgcc
     3061 cgccgtgtcc gccatcatcg tggcggcggg cgcgtcccgc cgaatgggat ttgataaact
     3121 cagcttccgt ctgccggatg gccgcaccgt gttggagaca agctgcgccg ccctggctgc
     3181 gcacccggct gtcacccagc tggtgctggt ctgcggcggc aaccgcgccg cctgcgaggc
     3241 aatcgccgcc gcctgcccca agccctgtgc cgttgtgcag ggcggggcca cccgcgccga
     3301 cagcgtgcgc aacggcctgg ccgccgccac cggggagctg gtcgcaatcc atgacgccgc
     3361 ccgccccttt atcagcgccg aggtcatcac cgcagcgctg accgctgccg ccgcttctgg
     3421 cgcggcggcc cccgctgtgc cggtcaagga caccatcaaa attgcgggca agtccggccg
     3481 cgtcgtcgag acgcccgacc gcgccgcgct ctacgccgtg cagacgcccc agtgctttca
     3541 gcgcacactg tatttgcagg cgctggatgc cgtcaccggc gaaaaagcga gccttgtcac
     3601 cgacgattgc agcctgtttg agctggcggg cttgccggtc acgctgacgg cgggggacta
     3661 cgccaactac aaaatcacca ccaaagagga cctacaaaag gagaaaacca tgcgcattgg
     3721 tcacggatat gatgttcacc gccttgtgga ggaccgcaag ctgattctgg gcggcgtcga
     3781 agtaccctac gagaagggac tgctcggcca ctcggacgct gacgtactgc tgcacgccgt
     3841 catggacgct gtgctgggcg cggcggcgct gggggacatc ggccagcatt tccctgatac
     3901 cgaccccgct tacaagggcg cggactcgct ggcactgacc cgcgaggtgg caaaaatcat
     3961 cgcccagcac ggctataaag tcggcaacat cgacgccacg attttgtgcc agcgccccaa
     4021 actggccccg cacatcccgg ccatgcgcca gaacattgcc gacgccttcg gcctgccgtt
     4081 ggacgccgtc agcgtgaagg ccaccactga ggaacacctc ggcttcaccg gcgagggcct
     4141 cggcatcgcc gcccacgcgg tggcgctgat
//
LOCUS       DBMDSQ010000177         4157 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_5000_length_4157_cov_1.948471, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000177 DBMDSQ010000000
VERSION     DBMDSQ010000177.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 4157)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4157)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4157
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5000_length_4157_cov_1.948471"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1560
                     /locus_tag="ACZPAX_06985"
     CDS             <1..1560
                     /locus_tag="ACZPAX_06985"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF012301.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HIR1113681.1"
                     /translation="AALHSGEIDAYYTYTYQAERTIFDDKKNELRSMLSDERRSFCIG
                     VNRDYDVLLRSVLNKSVNSLTRAEVVSITNKYINLGTQKFSLVRLAYQYPPVIVLTYL
                     CVVAAIVCIRLVLRSRRFRAEAEKALHKAEEASAAKTEFLSNMSHDIRTPINGIMGML
                     DIAEENFDDKARVKDCIAKMRGAASHLLSLVNDVLDMSKIESGSMQMLNTPFDLRVLL
                     DSCCGILEGQITERNMTFTKQIGPFWHPYLVGSELHIRQVLINILSNAVKYTPDGGEI
                     NFRAKETLFEEGLVHLRMEIKDNGIGMSEEFMQHIFEPFTQEQRTSRTTYKGTGLGMA
                     ITKKLVDQMHGSLDVESEPGKGSTFILRLSLPVGERPANAPDPMEEKRAPDLKDLHLL
                     LAEDNELNAEIAVTLLEEQGAKITAVTNGKEALEAIQNAAPRTYDAILMDVMMPEMNG
                     LEATRCIRAFEGKGPDEGTPIIAMTANVFADDVKACLDAGMNSHVGKPLDMKILMAEI
                     LKYTNRTRSIC"
     gene            1713..3359
                     /locus_tag="ACZPAX_06990"
     CDS             1713..3359
                     /locus_tag="ACZPAX_06990"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HIR1113682.1"
                     /translation="MATKYIFVTGGVVSGLGKGITAASLGRLLKTRGLKVASQKLDPY
                     INVDPGTMSPLQHGEVFVTDDGTETDLDLGHYERFIDENLNKYSNLTTGKVYWNVLNK
                     ERQGAYLGQTVQIIPHITNEIKSYIYNLAKSTEADVVITEIGGTTGDIESQPFLEAIR
                     QVGLEQGPENCCYIHVVLVPYISGSDEYKSKPAQHSCKELQGMGIAPNIIVLRADGPV
                     GNDIKRKISMFCNVRPDCVIENLTMPSLYECPLMLETAGLTNVVARQLHLQTPPTDLT
                     EWKELISRIATRSKTCTIALVGKYVKLHDAYLSVMESLYHAGFENESKVNIKWVDSEH
                     LVDQNCCAEELGDADGIIVPGGFGDRGIEGMIQAAQYARENHVPYFGICLGMQIMVME
                     YARNVLGYADANSSEFTPEGHHNVIDLMPDQQGVETKGGTMRLGKYPCTITPGTRMAE
                     CYGTLNIDERHRHRYEFNNEFRAEFEEKGLVIAGTSPDGRLVEAVEIPGRDFHLGAQF
                     HPEFKSRPNRAHPLFKGFIAAALKYQSEHTPTDHPMSLGE"
     gene            3602..>4157
                     /locus_tag="ACZPAX_06995"
     CDS             3602..>4157
                     /locus_tag="ACZPAX_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005924360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HIR1113683.1"
                     /translation="MRNWCSRHPVWFMALYALFYLTFFSLLEHTVLTPDLWVHCRLDD
                     VIPFCKYAIVPYLLWFPWIPFTLFYLLYKAPREDFWRLCVPLFTGMTMALLFYVIVPN
                     GLALRPHFVPGNDIFAQLVRMIYRSDTPMNVCPSIHVFNSVTLMLAYYRSAVFDAPRR
                     RWMRPAAMVLCIFLHVWSGSSGGDT"
BASE COUNT          900 a         1219 c         1187 g          851 t
ORIGIN      
        1 gcggcgctgc acagcggcga aattgacgcc tactacacgt acacctacca ggccgagcgg
       61 accatctttg acgacaaaaa gaacgagctg cggtcgatgc tcagcgacga gcggcgcagt
      121 ttttgcatcg gcgtcaaccg cgattacgac gtgctgctgc gcagtgtgct gaacaagagc
      181 gtaaacagcc tgacccgcgc cgaggtcgtc tccatcacca acaaatatat caacctgggc
      241 acgcagaaat tctcgctggt gcgcttggcg taccagtatc cgccggtcat tgtgctgacc
      301 tacctgtgcg ttgtcgccgc aatcgtctgc atcaggctgg tgttgcgcag ccgccgtttc
      361 cgcgccgagg ccgagaaggc cctgcacaag gctgaggaag ccagtgctgc caagacggaa
      421 tttttgtcga atatgtccca cgatatccgc acacccatca acggcattat gggtatgctg
      481 gacattgccg aggaaaattt tgacgataaa gcacgtgtca aggactgcat agccaagatg
      541 cgcggtgcgg cgtcccacct gctctcgctg gtgaacgacg tgctggatat gtccaaaatc
      601 gagtccggca gtatgcagat gctcaacacc ccgtttgatt tgcgggtgct gctggattcc
      661 tgctgcggca ttttggaagg gcagatcacc gagcggaaca tgacgttcac gaagcagatc
      721 ggcccgttct ggcatcccta cctggtgggc agtgagctgc acatccgcca ggtgctcatc
      781 aacattctgt ccaacgccgt caagtacacg cccgacggcg gcgagatcaa ctttcgcgcc
      841 aaggagacgc tgtttgagga aggccttgtc cacctgcgca tggagatcaa ggacaacggc
      901 atcggcatga gcgaggagtt catgcagcac atctttgagc cgttcaccca ggaacagcgc
      961 acgagccgca caacctacaa gggcaccggc ctgggcatgg ctatcacgaa aaaactcgtt
     1021 gaccagatgc atggctcgct ggatgtggag agtgaacctg ggaagggttc gacctttatt
     1081 ttacggctgt cgctgccggt gggcgagcgc cccgccaacg cccctgaccc gatggaggaa
     1141 aagcgtgcgc ctgatttgaa ggacctgcac ctgctgctgg ccgaggacaa tgagctgaac
     1201 gctgagatcg ccgtgacgct gttggaggaa cagggcgcga agatcaccgc tgtgaccaac
     1261 ggcaaggagg cactggaggc catccagaac gccgcgcccc gcacctacga cgcgattctg
     1321 atggacgtga tgatgcctga gatgaacggt ttggaagcta cccgctgcat ccgcgcgttt
     1381 gagggcaaag gccccgacga gggcacgcct atcattgcca tgacggccaa cgtctttgcc
     1441 gacgatgtaa aggcctgctt ggatgcgggc atgaacagcc acgtaggtaa gccgctggac
     1501 atgaagatcc tgatggccga aattttgaag tacacgaacc gcacccgctc gatttgctga
     1561 agaatctaaa gtaaaacccg cttttttcaa attagattaa gaaaatgtgc ccgccatatt
     1621 gcaaaaacta ttttttcgga tgtataatag gaaggatagt ttttgtggta tggcgggtgc
     1681 ttttgtgtgc ccccgaacat ggagggtgaa ttatggcaac gaagtatatt tttgtgaccg
     1741 gcggcgttgt gtcgggctta ggcaagggca tcacagcggc cagcctgggc cgtctgctca
     1801 agacccgcgg gctgaaggtg gccagccaga agctggatcc ctacatcaat gtggaccccg
     1861 gcacgatgag ccccttgcag cacggcgagg tgttcgtgac ggacgacggc accgagaccg
     1921 acctggactt gggccattat gagcgtttta tcgatgaaaa cctgaacaaa tactccaacc
     1981 tgaccaccgg caaagtctac tggaacgtcc tgaacaagga gcggcagggc gcctacctgg
     2041 gccagaccgt gcagatcatc ccccacatca ccaacgagat caagagctac atttataacc
     2101 tggccaagag caccgaggcc gatgtcgtta tcaccgagat cggcggtacc accggcgata
     2161 tcgagagcca gccctttctg gaggccatcc gccaggtcgg cctggagcag ggccctgaga
     2221 actgctgcta catccatgtc gtgctggtac cctacatttc cggctccgac gagtacaaat
     2281 ccaaaccggc ccagcattcc tgcaaggagc tgcagggcat gggcatcgcg ccgaacatca
     2341 tcgtcctgcg cgccgacggc cctgtgggca acgacatcaa gcgcaagatc agtatgttct
     2401 gcaacgtgcg ccccgactgc gtcattgaga acctgacgat gccgagcctg tacgagtgcc
     2461 ccctgatgct ggaaaccgcc ggcctgacca acgtagtggc ccgtcagctg cacttgcaga
     2521 ccccgcccac cgacctgacc gagtggaaag aacttatcag ccgcattgcg acccgcagca
     2581 agacctgcac catcgcgctg gtgggcaagt acgtcaaact gcacgacgcc tacctgagcg
     2641 tcatggagag cctgtaccat gcaggttttg agaacgagag caaggtcaat atcaagtggg
     2701 tggacagtga gcacctggtg gaccagaact gctgcgccga ggaactgggc gacgccgacg
     2761 gtatcatcgt gccgggcggc ttcggcgacc gcggcattga gggcatgatc caggctgccc
     2821 agtacgcgcg ggagaaccat gttccctact ttggcatctg cctgggtatg cagatcatgg
     2881 tcatggagta cgcccgcaat gtgctgggct acgccgacgc caattccagc gagtttacgc
     2941 ccgagggcca tcataacgtc attgacctga tgccggatca gcagggtgtc gagacgaagg
     3001 gcggcacgat gcgcctgggc aagtacccct gcaccatcac cccgggcacc aggatggccg
     3061 agtgctacgg cacgctgaac atcgacgagc gccatcgcca ccgctatgag ttcaacaacg
     3121 agttccgcgc cgagtttgag gaaaagggcc tggtcattgc aggcaccagc ccggacggcc
     3181 gcctggtcga ggctgtcgag attccgggcc gtgacttcca cctgggtgcc cagttccacc
     3241 ccgagttcaa aagccgcccg aaccgcgccc acccgctgtt caagggcttc atcgccgccg
     3301 cgctgaagta ccagagcgag cacaccccaa ccgaccatcc catgtcgctg ggggaataaa
     3361 cttgtaataa tagagccttc ccccgagggg gaaggctttt tgctgcccat caatttacaa
     3421 ctctttcaca ttttccccgc aatatcccct gccttcgatt cgtactgtta tatagagcag
     3481 gctcacacaa aaaaaccgcc aatatcctgt caaaaacccg cgtatttctt gtgcggattt
     3541 tcgcttgctt tatgcgcccc tggcggcgta taatggtaca tcatcgaaag ggtgtaaatc
     3601 tatgcgtaac tggtgttcgc gccatccggt ctggttcatg gcgctctatg ctttgtttta
     3661 cttaacgttt ttctcgctgc tggaacacac cgtcctgacg ccggacctgt gggtccactg
     3721 ccggttggac gatgtgattc ctttctgcaa atatgccatt gtaccgtatc ttttgtggtt
     3781 cccgtggatc ccgttcacgc tgttctacct gctgtacaag gccccgcggg aggatttttg
     3841 gcggctgtgc gtgccgctct tcaccggcat gacgatggcg cttttgttct acgtcatcgt
     3901 gcccaacggc ctggcgctgc gcccgcattt tgtgccgggc aacgacattt tcgcgcagct
     3961 ggtgcggatg atctaccgca gcgacacgcc gatgaacgtc tgtccgtcca tccatgtctt
     4021 caactcggtc acgctgatgc tggcctacta ccgcagtgcg gtttttgacg ccccgcgccg
     4081 ccgctggatg cgccccgctg ccatggtgct gtgtatcttt ttacatgttt ggtctgggag
     4141 cagtggtggt gacactg
//
LOCUS       DBMDSQ010000178         4154 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_5005_length_4154_cov_1.892179, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000178 DBMDSQ010000000
VERSION     DBMDSQ010000178.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 4154)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4154)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4154
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5005_length_4154_cov_1.892179"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..888)
                     /gene="ilvC"
                     /locus_tag="ACZPAX_07000"
     CDS             complement(<1..888)
                     /gene="ilvC"
                     /locus_tag="ACZPAX_07000"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015565506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HIR1113684.1"
                     /translation="MAINKYYDSDCNLGVLDGKTVAVIGFGSQGHAHSENLAESGVNV
                     VVGLRKGSAHWEKASEFAATHENFKVMEVEEAAKAGDVVMMLVPDELCADIYNSQVAP
                     YMTEGKALAFAHGFNIHFKTITPPKNVDVIMIAPKGPGHIVRRLYTEGEGCPSLICVE
                     QDYTGKAKEIALAYASGIGAGRAGILETTFKEETETDLFGEQAVLCGGVCELIQAGFD
                     TLVEAGYAPEMAYFETCHEMKLIVDLINEGGFSKMRYSISNTAEYGDYRTGKRLITED
                     TRKEMKQVLKEIQDGTFASE"
     gene            complement(1053..1622)
                     /gene="ilvN"
                     /locus_tag="ACZPAX_07005"
     CDS             complement(1053..1622)
                     /gene="ilvN"
                     /locus_tag="ACZPAX_07005"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007045719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HIR1113685.1"
                     /translation="MEQNNNTRRVISLLVDNSNGVLARISSLFCRRGFNIDSLTVSAT
                     NDPKVSRITVTLRGSEQAMTQLILQTERLEVTRQVFELDPDKSLQRELLLLKVACDNQ
                     NRAELREISSIYKAKIIDLSPDSMVFELTGKPDKIDAFLTMFKGYEILELCRTGVTAL
                     ERGGKHQHMQKPAQEVREAQLPLPGTHCH"
     gene            complement(1633..3297)
                     /gene="ilvB"
                     /locus_tag="ACZPAX_07010"
     CDS             complement(1633..3297)
                     /gene="ilvB"
                     /locus_tag="ACZPAX_07010"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015537499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HIR1113686.1"
                     /translation="MKLTGSQIFVEVLVEQGVDTLFGYPGGAVLNLYDELYKNSDRIR
                     HVLTAHEQGASHAADGYARATGRTGVVLATSGPGATNLVTGIATAYMDSVPMVAFTGN
                     VATTLLGKDSFQEAYIEGITMPITKHNYTVRRVEDLADTMRAAFRIAQSGRKGPVLVD
                     IPKDITAASCEFTPKAPELIRTVTYYNEEDVKEAARLINESERPIVYFGGGVRSAAGC
                     QPLRDLLEKTGMPATYTLMAAGVLSYGEPHNLGLLGMHGCYAANKAIDEADLVIAVGT
                     RFSDRVALNPDSFAKRAKIIQIDIDPSELGKNVDVDLSLTGDASYILQAILPYVEKTE
                     HKFWMEQIRGWQKNDYKPVDSDTELKPHQIIDEICSQAGPEAVYVTDVGQHQMWAAQY
                     LHHTKSRGFLTSGGLGTMGFGYGAAIGAQMALGNDARVVMLTGDGSFHMNLNEACTAV
                     SYGLPIITVIFNNQVLGMVRQWQTTFYEKRYSDTDPHRRTDFVKLAEGFGAKGYRATT
                     PAEFKAAFADAMQQKGPSWIDCRIGKDEKVLPMIPGGGTINDIIME"
     misc_binding    complement(3420..3664)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3804..>4154
                     /locus_tag="ACZPAX_07015"
     CDS             3804..>4154
                     /locus_tag="ACZPAX_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007045716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR1113687.1"
                     /translation="MLRNEWLAHLDGLVVFRGLLAAEPLCTLRAALAADDAALTGAVA
                     AFEAALFARGTNWTDALLEAVLEDENLCLKVAAGQDAGPVLEKAMDDELDFLQALGRA
                     PLDEVFPGAAAFLPR"
BASE COUNT          739 a         1183 c         1300 g          932 t
ORIGIN      
        1 ctcggaggca aaggtgccgt cctggatctc cttcaggacc tgcttcatct ccttgcgggt
       61 gtcctcggtg atgaggcgct tgccggtgcg gtagtcgccg tactcggccg tgttggagat
      121 ggagtagcgc atcttggaga agccgccctc gttgataagg tcaacgatga gcttcatctc
      181 gtggcaggtc tcaaagtagg ccatctcagg ggcgtaaccg gcctcgacca gcgtgtcaaa
      241 gccagcctgg atcagctcgc agacaccgcc gcagagcaca gcctgctcgc cgaacaggtc
      301 agtctcagtc tcctccttga aggtggtctc caggatgccg gcacgaccgg cgccgatgcc
      361 ggaagcgtaa gccagggcaa tctccttggc cttgccggtg tagtcctgct caacgcagat
      421 cagagacggg cagccctcgc cctcggtgta caggcggcgg acgatgtggc cggggccctt
      481 gggggcaatc atgatgacgt caacgttctt cggcggggtg atggtcttga agtgaatgtt
      541 gaagccgtgg gcgaatgcca gggccttgcc ctcggtcatg tagggggcaa cctgagaatt
      601 gtagatgtcg gcgcacagct cgtcgggaac cagcatcatg acgacgtcac cggccttggc
      661 ggcctcctcg acttccatga ccttgaaatt ctcgtgggtg gcggcaaact cggaggcttt
      721 ctcccagtgg gcagaaccct tgcgcagacc aacgacgaca ttgacgccgc tctcggccag
      781 gttctcggaa tgggcgtggc cctgggagcc gaagccgatg acggcaacgg ttttgccgtc
      841 cagcacgccg aggttgcagt cggaatcgta gtacttgttg atagccattg tggtatctcc
      901 ttttgttgtt gatacttttt atatttgtaa ttttaattgg gaaacgaaaa taaaaatccg
      961 taggggcgca ttctatatgc gcccatgcag tgcagcctac ggcgggcgga tatggaatcc
     1021 gcccctacag gcattgaaaa tttacgagca gctcaatggc agtgcgtccc cggcagcggc
     1081 agctgtgcct ctctgacttc ctgcgcgggt ttctgcatat gctggtgctt gccgccgcgc
     1141 tcgagagcag tgacgccggt gcggcaaagc tccagaatct catagccctt gaacatcgtc
     1201 aggaaagcgt cgattttatc cggcttgccg gtcagctcaa ataccatgct gtcaggcgac
     1261 aggtcgatga ttttcgcctt gtagatgctg gaaatctccc gcagctcggc gcggttttgg
     1321 ttgtcgcagg ctacctttaa cagcagcagc tcgcgctgta agctcttgtc ggggtccagc
     1381 tcaaagacct ggcgggtgac ttcgaggcgt tccgtctgca aaatcagctg ggtcatggcc
     1441 tgctcgctgc cgcgcagcgt gaccgtaatg cggctgactt tggggtcatt cgtagccgag
     1501 acggtcaggc tgtcaatgtt gaagccacgg cggcagaaca ggctggaaat gcgggccaga
     1561 acgccgttgg agttatcgac cagcaggctg atgacccgcc gggtgttgtt gttttgttcc
     1621 atgcgtgtca cctcattcca tgatgatatc gttgatggtg ccgccgccgg ggatcatcgg
     1681 aaggactttt tcatccttgc cgatgcggca atcgatccag ctcgggcctt tctgctgcat
     1741 ggcgtcggcg aaagccgcct tgaactcggc gggagtcgtg gcgcggtagc ccttggcccc
     1801 aaagccctcg gccagcttga caaagtcggt gcggcggtgc gggtcggtgt cagagtagcg
     1861 cttctcgtag aaagtcgtct gccactggcg caccatgcca agcacctggt tgttgaagat
     1921 gactgtgata atgggcaggc catagctgac ggcagtgcag gcctcgttca ggttcatgtg
     1981 gaaggagccg tcgccggtca gcatgacgac gcgggcatcg ttgcccagcg ccatctgtgc
     2041 gccgatggcc gcgccgtagc cgaagcccat cgtgcccaat ccgccgctgg tcaggaaccc
     2101 acggctcttg gtgtggtgca ggtattgcgc cgcccacatc tggtgctggc cgacgtcggt
     2161 gacgtagacg gcctcgggac cggcctggct gcagatttcg tcgatgatct gatggggttt
     2221 cagctcggta tcgctgtcca ccggcttata atcgttcttc tgccagccgc ggatctgctc
     2281 catccagaac ttgtgctcgg tcttttcgac gtagggcaga attgcctgca aaatgtagct
     2341 ggcgtcgccg gtcaggctca aatcgacgtc gacgttcttg cccagctcgc tggggtcgat
     2401 gtcgatttga atgatttttg cgcgcttggc aaagctgtcg gggttcagcg ccacgcggtc
     2461 actgaaccgc gtgcccacgg cgatgacgag gtcggcctcg tcgatggcct tgttggcggc
     2521 gtagcaaccg tgcataccca gcaggcccag attgtgcggc tcgccgtagc tgagaacacc
     2581 cgcggccatc agggtgtagg tggcgggcat accggtcttt tccagcaggt cgcgcagggg
     2641 ctggcagccc gccgcactgc gcacaccgcc gccgaagtag acgatggggc gctccgactc
     2701 gttgatgagg cgggcggctt ccttgacgtc ctcttcatta taataggtca cggtgcggat
     2761 gagttccggc gctttggggg tgaactcgca ggacgccgcc gtgatatcct tcgggatatc
     2821 gacgagcacc gggcctttgc ggccggactg tgcaatgcgg aaggccgcac gcatcgtgtc
     2881 ggccagatcc tctaccctgc gcactgtgta gttgtgcttg gtgatgggca ttgtgatgcc
     2941 ctcaatgtag gcctcctgga aggagtcttt acccagcagc gtcgtggcga cattgccggt
     3001 gaaggcgacc atcggcacgc tgtccatgta ggccgtggca atgccggtga caaggttcgt
     3061 cgcacccggg ccgctggtgg ccagcacaac gccggtacgg cccgtggcgc gggcgtaacc
     3121 gtccgccgcg tgggacgcgc cctgctcgtg ggcggtcaat acgtgacgta tacgatcaga
     3181 gtttttatac aactcgtcat acagattcag caccgcgccg cccggatagc cgaacagggt
     3241 atcgacgccc tgctcgacca acacttcgac gaagatctgt gaaccggtca atttcatggt
     3301 gtttgctcct tcctacttcc tgtgcgactt ccccggggca tccgtatggc ggggttccct
     3361 caccccgctg cggcccggta aggggcaata taagaaaaca ggctttgccc cttgcttttc
     3421 caagagacaa agcctgtgcg tttcacatac tctgcggtac cactctcatt ggggcgttgc
     3481 ccccacctca tgaacctcta acaaggtccg gcactgtaac ggatgcgccc gtaccgccct
     3541 actgtgattt tcgggtggtc tgctccggga tcagttcgcg gcttgcctgc cttgctgcct
     3601 tgcaccgacc ggcagttctc tgaaaggggc ggggccgtta ctaacttccc atccaagcat
     3661 tatctggatt tatggtaaac tataccgtac ttttgcatgt ttgtcaagac ttctttgaaa
     3721 attctgcaac aaaccttgca actttttgtc gcggcagtta tactggggta aagagtgttt
     3781 ctcttgggaa aggattataa actatgttac gtaatgaatg gctggcccat ctggatgggc
     3841 ttgtggtgtt tcgcggcctg ctggcggccg agccgctgtg cacgctgcgt gcggcgctgg
     3901 cggcggacga tgccgccctg accggcgcag tggccgcgtt tgaggcggcg ctgtttgccc
     3961 gcggcaccaa ctggaccgat gccctgctgg aagcggtgct cgaggatgag aacctttgtt
     4021 taaaggttgc cgccgggcag gatgccgggc cggtgctgga aaaggcaatg gatgatgaat
     4081 tggactttct gcaagcgttg ggccgtgcac cgctggacga ggtgttcccc ggtgcggcag
     4141 cgttcctgcc ccgg
//
LOCUS       DBMDSQ010000179         4143 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_5028_length_4143_cov_1.855022, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000179 DBMDSQ010000000
VERSION     DBMDSQ010000179.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 4143)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4143)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4143
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5028_length_4143_cov_1.855022"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..620)
                     /locus_tag="ACZPAX_07020"
     CDS             complement(<1..620)
                     /locus_tag="ACZPAX_07020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113688.1"
                     /translation="MRILFVLDRVENAASANALMGFRLAEQLVACDHSVHILTLWDGL
                     HTPPAPPEGVVQHLLAFADERLMNEALENGRRGGTPVPLRLARLAAHPTAVAAAFRQL
                     VLKRPRRAVDTQREIERLDAEFHYDAVCAVCAPYRAAFALEEASIGGKKYLWQLDPYA
                     SNRDYTAPGGFAREGRLLDALDGTFITPQAAPDYADDAPLANWCEKT"
     gene            complement(621..1610)
                     /locus_tag="ACZPAX_07025"
     CDS             complement(621..1610)
                     /locus_tag="ACZPAX_07025"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR1113689.1"
                     /translation="MKPCEIAVIVPVHNAAAFLTACTDAIAAQKFDAFRCILVDDGST
                     DGSPALCDAIAAGDQRFVAIHQPNSGVCAARTAGLRRAQELGARWYAFCDADDLYHPE
                     LLDTLYAAVTNSGLPLACCRYDTFTDAAPANSAVPSNCKVLRSPAHLDALLHDHAVDY
                     GLWNKLFSADLLTEEMLDNGLAYNEDLLANWRAFLAAPGCAYCDFSGYHYRQHAASAS
                     HRALPPQSIDDQRRAAAEIRATAPDALRESVNAFYYEKLVYLASMILRRANADDYRTQ
                     LCELKVAIAAGEKDPQLGRNPQLPRSIQLAAFLTLHAPRLWRWACKNFLKDRQ"
     gene            complement(1607..2722)
                     /locus_tag="ACZPAX_07030"
     CDS             complement(1607..2722)
                     /locus_tag="ACZPAX_07030"
                     /inference="COORDINATES: protein motif:HMM:NF024831.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HIR1113690.1"
                     /translation="MPPIRVLQIMPAMDAGGMETFVMNVYRTIDREKVQFDFLYHYDK
                     PCFFDDEIRALGGRIYKLTVRQDNNIPRYLRELRTLFAAHPEWRIIHGHYSGFGMFYN
                     PVAKRAGIPVRCGHSHNTAYEPNLVGQLDRVMSSFFRFGLTDRFACSEKAGQMLYGKA
                     PFTVVPNGIDTARFAARDAQRRALLRGELGVTDQEILFGHVGRFSAQKNHPGLLKIFA
                     AVRTRLPKARLVLLGGGEPAYVEKMQNLAAELGLGDSVIFAGVRSNMQSFYDAMDAFM
                     LPSLFEGLPVVLVEAQTAGLPCFVSDTVDAGAAFTDRVKFLPLNDVSAWTNTIAAASF
                     RRDPQAREKAVKAGYDIHTSAQVLQQFYLRRYAEVTK"
     gene            complement(2722..3591)
                     /locus_tag="ACZPAX_07035"
     CDS             complement(2722..3591)
                     /locus_tag="ACZPAX_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009323419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006797 - polyphosphate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PPK2 family polyphosphate kinase"
                     /protein_id="HIR1113691.1"
                     /translation="MKADKYRFDGSRACDLRALSCDGKDDAPDKDEIIAKTAENLQKM
                     AALQDAFYADGREGLIIVLQALDAAGKDGTVKHVMSGLNPQGVQVTSFKQPNSEELKH
                     DFLWRVNKALPPRGTIAIFNRSYYEDVLVVQVHDLQKTYQMADRVLGESKKAFFKKRY
                     RQIRNYEEYLFDNSYRIVKIFLNISKKEQKKRFLERIDNPAKNWKFSSSDLAERAHFD
                     EYLDVYTDVIDATATPESPWYALPADRKWYTRYLVSEIVLDALQQCSHTYPQLDAAAQ
                     AALKDCRTQLEAE"
     gene            complement(3787..3939)
                     /locus_tag="ACZPAX_07040"
     CDS             complement(3787..3939)
                     /locus_tag="ACZPAX_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HIR1113692.1"
                     /translation="MASVIVVVIIFVLLALAILFICQNGGWQGGCGGNCAGCHNKCAE
                     SEKKKK"
     gene            complement(3968..>4143)
                     /locus_tag="ACZPAX_07045"
     CDS             complement(3968..>4143)
                     /locus_tag="ACZPAX_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015260841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HIR1113693.1"
                     /translation="VFLIFTLLYTPCVAAIAAVKRELGGAKAAAGVVLMQCAIAWGVA
                     FVVHCVGSLIGFV"
BASE COUNT          857 a         1222 c         1230 g          834 t
ORIGIN      
        1 gtcttttcgc accaattcgc cagcggggca tcgtcggcat agtcgggcgc ggcctgcggc
       61 gtgatgaagg tcccatccag cgcgtccagc agccgaccct cccttgcaaa gccgcccggg
      121 gccgtgtagt cccggttgct ggcgtagggg tctagctgcc acaggtactt tttaccgccg
      181 atggacgcct cttccagcgc aaaggccgcg cggtagggcg cacagacagc acagacggcg
      241 tcatagtgaa attctgcgtc cagccgctcg atctcccgct gcgtatccac ggcgcggcgg
      301 ggccttttca gcaccaactg gcggaacgct gccgccacag ccgtggggtg ggcggccagc
      361 cgggccagcc gcagcggtac cggcgtgccg cctctgcggc cgttttccag cgcctcattc
      421 atcaggcgct cgtcggcaaa ggccagcaga tgctgcacga ccccttccgg cggggcaggc
      481 ggggtgtgca ggccgtccca caaggtcaaa atatgtacgg agtggtcgca ggcaaccagc
      541 tgttccgcca gtcggaaccc catcagggcg ttggcgctgg cggcgttctc cacccggtcc
      601 agtacgaata aaatgcgcat ttattgtcgg tccttcaaga aatttttgca cgcccaccgc
      661 cacaaacgcg gcgcgtgcag cgtcaaaaat gccgcaagct gtatgctgcg cggcagctgc
      721 gggttgcggc ccagctgggg gtctttttcc ccggctgcaa tcgccacctt tagctcacac
      781 agctgggtac ggtaatcgtc ggcgttggca cggcgtaaaa tcatgctggc caggtagacg
      841 agcttttcat aataaaaggc gttcacgctc tcgcgcaggg cgtcgggggc ggttgcgcgt
      901 atctcagcag cggcgcggcg ctggtcgtcg atgctctgcg gcggcagggc gcggtggctg
      961 gcgctggccg cgtgctggcg gtagtggtag cctgaaaaat cgcagtaggc acaccctggt
     1021 gctgccaaaa atgcacgcca gttcgccaat aaatcttcat tgtaggccag gccattgtcc
     1081 agcatttcct cggtcagcaa atcggccgag aacagcttgt tccacaggcc gtagtccacg
     1141 gcgtggtcat gcagcagcgc gtccagatgc gcggggctgc gcaggacctt acagttggac
     1201 ggtacggcag aatttgccgg tgcagcgtcg gtaaatgtgt catagcggca gcaggccagc
     1261 ggcaggccgc tattggtcac ggcggcgtac agcgtgtcca gcagttccgg gtgatacaga
     1321 tcgtccgcgt cgcaaaaggc ataccaccgt gcgcccagct cttgggcacg gcgcagtccg
     1381 gctgtacggg ccgcgcagac accgctgttg ggctggtgta tggccacaaa ccgctggtcg
     1441 cccgccgcaa tggcatcgca cagggcgggg gaaccatccg tggagccgtc gtccaccaaa
     1501 atgcaccgaa aggcgtcaaa tttttgtgcg gcaatggcat cggtgcaggc ggtcaaaaac
     1561 gccgccgcgt tatgtaccgg cacaatgact gcaatctcgc agggcttcat ttcgtcacct
     1621 cggcatagcg gcgcaggtaa aattgttgca gcacttgggc gctggtgtgg atatcatacc
     1681 cagccttcac ggccttttcc cgcgcctgcg ggtcacggcg gaaagatgcc gccgcgattg
     1741 tatttgtcca ggcggaaaca tcgttcagcg gcaaaaattt cacccggtcg gtaaaggccg
     1801 cgcccgcgtc caccgtgtca gacacaaagc agggcaggcc cgcggtctgg gcttccacca
     1861 gcacgacagg gagcccctca aacaggctgg gcagcataaa ggcgtccata gcatcataaa
     1921 aactttgcat attgcttcgc acaccggcaa aaatcacgct gtcgcccagg cccagctcgg
     1981 cggccagatt ttgcattttt tccacgtagg caggttcacc gccgcccagc agcaccagcc
     2041 gcgccttggg cagacgtgta cgcactgcgg caaaaatttt cagcagtccg gggtggtttt
     2101 tctgggccga aaaccgcccc acgtggccga acaaaatctc ctggtcggtc acgcccagtt
     2161 cgccccgcaa aagagcgcgg cgctgggcat cgcgggcggc aaatcgcgcc gtgtcaatac
     2221 cgttgggtac tacggtaaac ggcgctttgc cgtacagcat ctgcccggct ttctcgctgc
     2281 aggcgaagcg gtcggtcagg ccaaagcgga aaaagctgct catgacgcgg tccagctggc
     2341 ccaccaggtt cggctcatag gcagtgttgt ggctgtggcc gcaccgtacc gggatgcccg
     2401 cccgcttggc cacagggtta taaaacatcc caaacccgct gtaatggccg tggatgatgc
     2461 gccactccgg gtgggcggca aacagggtgc gcagctcgcg cagatagcgg gggatgttat
     2521 tgtcctgccg gacggtcagc ttgtaaatac ggccgcccag ggcacgtatc tcatcgtcaa
     2581 aaaagcacgg tttatcgtaa tggtagagga aatcaaactg caccttttcg cggtcgatgg
     2641 tgcggtatac gttcatgacg aacgtctcca ttccgccggc gtccatggcg ggcatgatct
     2701 gcaaaacacg gataggcggc atcactcggc ctccagttgg gtgcggcagt ccttgagcgc
     2761 ggcctgtgcg gcggcgtcca gctgcgggta ggtgtggctg cactgctgca aagcgtccag
     2821 cacaatttca ctgaccagat agcgggtgta ccatttacgg tcggcgggca gcgcatacca
     2881 ggggctttcc ggcgtggcag tggcgtcgat gacatccgtg tatacgtcca ggtattcgtc
     2941 aaaatgcgcc cgctcggcca gatcgctgga cgagaatttc cagttcttgg ccgggttgtc
     3001 gatgcgctcc aaaaaccgct ttttctgctc tttcttggag atgttgagga aaattttcac
     3061 aatgcggtag ctgttgtcga aaagatactc ctcataattt ctgatctggc ggtagcgctt
     3121 tttaaaaaat gcctttttgc tctcgcccag cacgcggtcg gccatctggt aagtcttttg
     3181 caggtcgtgt acctgcacca caagcacatc ctcatagtag ctgcggttga agatggcgat
     3241 ggtcccgcgc gggggcagcg ctttgttgac gcgccagagg aagtcgtgct tcaattcctc
     3301 gctgttgggc tgtttaaagc tcgtgacctg cacgccctgg gggttcagcc cggacataac
     3361 gtgtttgacc gtgccgtcct tgcctgcggc gtccagcgcc tgcaaaacaa tgataagccc
     3421 ttcgcgccca tcggcgtaaa aggcgtcctg caaagcggcc attttttgca gattctcggc
     3481 cgttttggct ataatttcgt ccttgtcggg cgcgtcgtcc ttgccatcgc aggacagcgc
     3541 gcgcagatca caggcgcggg agccgtcaaa gcggtattta tcggctttca tgggcagaac
     3601 ctccccaaaa tatagatata aatagtatac ccggttttga tggattgtgc aaccttggca
     3661 gcaagaaaaa aggccgggca ttaagcatag gggagtagaa acgcgaaacc cggaaaggaa
     3721 aacaagatgg gaaaacagag ccatttgaaa aaggctttcc ccgcggggaa agcctttaca
     3781 agtgcgttac ttctttttct tctcgctctc ggcgcatttg ttgtggcagc cggcgcagtt
     3841 gccgccgcag ccgccctgcc agccgccgtt ctggcagata aacaggatcg ccagcgccag
     3901 caaaacaaaa ataattacaa caacaatgac acttgccatg taggatacct cgatttattg
     3961 tattgtgtta cacaaaacct attaaactgc ctacacagtg taccacaaat gccacgcccc
     4021 atgcaatagc acattgcatc aaaactaccc cggcagcggc cttagcacca cccagctcac
     4081 gcttgacagc cgcaatggcg gccacacagg gcgtatacag caaggtaaat atcaggaata
     4141 cga
//
LOCUS       DBMDSQ010000180         4142 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_5030_length_4142_cov_1.865627, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000180 DBMDSQ010000000
VERSION     DBMDSQ010000180.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 4142)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4142)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4142
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5030_length_4142_cov_1.865627"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(105..1106)
                     /locus_tag="ACZPAX_07050"
     CDS             complement(105..1106)
                     /locus_tag="ACZPAX_07050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113694.1"
                     /translation="MTNDRDEEFLRTKLTDGLHPAEVDLWPAVAEALPNARTAWPWRR
                     IALCLAAPLLIAAGVLAGTAGFTNLRENPRPSFAPEANTGYAITYERQYFTLDSDLLA
                     GLTANHNGEVSADGWLPMHCGINGFLHLPDEDEMNRYSRAVRGYDSWGELTAATDLPL
                     AQNALLDTEANNFAAVSLYPSGMIEIPEEYDGRPYTPKAQEYPAGLIPVGRGETPDKL
                     YLRTGARHGIYDVEMMAVVYLGDAAPETVAQLYRQKSAGLTWECESYPMANGNTALIP
                     HCTSNPNSMPYVQSSAYFAQDGILYQVYCRPHTDGLDYSVKQEDIWAELKVVLNGFT"
     gene            complement(1096..1581)
                     /locus_tag="ACZPAX_07055"
     CDS             complement(1096..1581)
                     /locus_tag="ACZPAX_07055"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR1113695.1"
                     /translation="MQTEQDLEKLVHAHTAALLRYCTGLLGCEADAQDAVQKTFIKAW
                     QQRRTLRGDGTDNERAWLYRIAYRTALDMLRAARRAANYPTPEPREQDPGISDELRTA
                     LAALPPLDRALVLERVLDGLDYAALAHIHGRPAAYLRTRYHRAKKQLASRLREGEHHD
                     E"
     gene            complement(1757..2851)
                     /locus_tag="ACZPAX_07060"
     CDS             complement(1757..2851)
                     /locus_tag="ACZPAX_07060"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HIR1113696.1"
                     /translation="MAKQRDPVLDSMRGIGIVLMVLGHSGFPGTGFIYLFHMALFFML
                     SGWLFSLRGGLVHFAKRKVVTLWLPFVLANTAFTVLNNLFLRLNILTGDERILALPGN
                     IVTTPTTLKDIIGRTAHWCVFDGGTQLGGALWFIQALFQISILYAVVEFLLQKLLRGG
                     DTLIAQGLVSGVLLWVGWRCGLAGWNVWGLGIAASCYSLFYLGTVLRCTTRSDVKPLH
                     RAGLAAAAFVLLAVLGQFGSVGLAGNGYPGPIFLLAASLAGWVLVRQAACLAVAWPKF
                     SAVLAYLGRATMPIVILHFLSFKLVTWLGLLATGGEPYLLAAFPTYFTGGLWWLAYTV
                     VGLALPLAGDWVYKKVREAFPLGGRWPKAG"
     gene            complement(2904..3278)
                     /locus_tag="ACZPAX_07065"
     CDS             complement(2904..3278)
                     /locus_tag="ACZPAX_07065"
                     /inference="COORDINATES: protein motif:HMM:NF013629.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HIR1113697.1"
                     /translation="MTRQRALILDIMREKCPEHMTADEIFSHARQAMPHIARGTVYRN
                     LKLMELDGEIGHLEMPDGPDRYDANPSPHGHLLCESCGDLADLPVVGLIRDIESAIGT
                     EVRGYTLTIRYICPKCRAKNAQ"
     gene            3467..4009
                     /gene="rbr"
                     /locus_tag="ACZPAX_07070"
     CDS             3467..4009
                     /gene="rbr"
                     /locus_tag="ACZPAX_07070"
                     /EC_number="1.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007045590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016692 - NADH peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin"
                     /protein_id="HIR1113698.1"
                     /translation="MELRGSKTEKNLWEAFAGESQARNKYTYFASKAKKEGYEQISAI
                     FTETANNEKEHAKIWFKLLMENGEIPDTLTCLKMASAGENEEHTSMYPRMAAEAKEEG
                     FNQVAALFTMVAAIEKEHEERYKALAANIEAGKVYARETEQVWQCRNCGFVYIGQHAP
                     VKCPVCAHPQSYFELKSSNY"
BASE COUNT          891 a         1230 c         1251 g          770 t
ORIGIN      
        1 ttcctaaagg gagactcgct gcggttagct ttaactaacg ctaacaatat agcatgctat
       61 cccacagttg tcaagcgttt tttgcaaggt tttccaggtg cgtattatgt aaacccattc
      121 agcaccacct tcagttcggc ccagatatcc tcctgcttga cgctgtaatc caggccgtcc
      181 gtgtgtgggc ggcaataaac ctgatacaaa atgccatcct gcgcaaaata ggcgctggac
      241 tgcacatagg gcatcgagtt cgggttgctg gtgcagtgcg gaatcagcgc agtgttgccg
      301 tttgccattg ggtagctttc gcattcccag gtcagcccgg cgctcttttg gcggtagagc
      361 tgggccacgg tctccggcgc ggcgtcgccc agatagacga cagccatcat ttccacatcg
      421 tagatgccat ggcgcgcgcc ggtgcgcagg tagagcttgt ccggcgtttc accgcgccca
      481 acgggaataa ggccagccgg gtattcctgc gctttcggcg tgtaggggcg gccgtcgtat
      541 tcttcgggta tctcgatcat gccagacgga tacagcgaga cggcggcaaa attgttggct
      601 tcggtatcca gcagcgcgtt ctgtgccagc ggcaggtcgg tcgcagcggt cagttcgccc
      661 caactgtcgt agccgcgcac tgcgcgggaa tagcggttca tctcgtcctc atcaggaagg
      721 tgcaaaaatc cgttgatgcc gcagtgcatc ggcaaccagc cgtcggcgga aacctcgccg
      781 ttgtggtttg ccgtcagccc cgccaataag tcgctgtcca gcgtaaaata ctgccgctca
      841 tacgttatgg catacccggt gttcgcctcg ggcgcgaagg aggggcgcgg gttctcccgc
      901 aggttcgtaa atcctgctgt gcccgccagt accccggctg caatcaacag cggcgcagcc
      961 aggcagaggg cgatgcgccg ccagggccat gccgtgcggg cgttgggcag agcctcggcc
     1021 acggcgggcc agagatcgac ctcggccggg tgcaggccgt cagtcagctt cgtgcgcaaa
     1081 aattcttcat cgcggtcatt cgtcatggtg ctccccctct ctcaatcgtg aggccagctg
     1141 ttttttggcc cggtgatagc gtgtgcgcag gtaggcggcg ggccgcccgt ggatgtgtgc
     1201 cagcgcggca tagtccaggc cgtccagcac gcgctccaac accagcgccc ggtcgagtgg
     1261 cggcagagcg gccagcgcgg tacgcagttc gtcgctgatg ccggggtcct gttcccgcgg
     1321 ttccggcgtt gggtagttgg ccgcgcggcg ggctgcccgc agcatatcca gcgcggtgcg
     1381 gtaggcgatg cggtagagcc acgcgcgttc gttgtcggtg ccgtcgcccc gcagggtgcg
     1441 acgctgctgc catgccttga tgaaggtttt ctgcacggcg tcctgggcat cggcttcaca
     1501 ccccagcagg ccggtacagt agcgcagcag ggcggcagtg tgggcatgta cgagtttttc
     1561 caggtcctgt tcagtctgca taggctcgac ctcctttcac ccttataacg ccgatggagg
     1621 cggaaatata tcaaaaaaag agccccgaaa ggctcgaaaa ttttggcgct ttggcttctc
     1681 ccgagggaga agctgtccgc gaagcggact gatgaggggc gcgtttcctt atggggcggc
     1741 aaggcaaaac cgcccctcat ccggccttcg gccaccttcc cccaaggggg aaggcttccc
     1801 gcactttctt gtaaacccaa tcccctgcca gcggcaaagc caggccaacc acagtatacg
     1861 ccagccacca aaggccgccg gtgaaatagg tggggaacgc ggccagcagg tacggctcgc
     1921 caccggtggc cagcagccca agccaggtca cgagcttgaa gctcaagaaa tgcaaaatga
     1981 caatgggcat tgtggcgcgg cccaggtagg ccagcacggc gctgaatttc ggccacgcca
     2041 ccgccagaca cgcagcctgc cgcaccagca cccagcccgc cagactggcg gccagcagga
     2101 agatcggccc ggggtagccg ttgcccgcca ggccaacgga gccaaactgc cccagcacgg
     2161 ccagcagcac aaacgcggca gcagccagcc cggcgcggtg cagcggctta acgtcgctgc
     2221 gggtggtgca gcgcagcaca gtccccaggt agaacaggct gtaacagctg gccgcaatgc
     2281 caagccccca gacgttccac ccggccagac cgcagcgcca gcccacccag agcagcacgc
     2341 cggagacaag cccctgggca atcagtgtat caccgccgcg cagcagcttc tgcagcaaaa
     2401 attccacgac agcgtacaaa atcgaaatct ggaacagcgc ctggatgaac cacagcgccc
     2461 cgcccagctg ggtgccgccg tcaaatacgc accagtgcgc cgtgcggccg atgatgtctt
     2521 tcagcgttgt gggcgtggtg acgatattgc cgggcagcgc cagaatgcgc tcatccccgg
     2581 ttaaaatatt caggcgcagg aacaggttgt tcagcaccgt gaacgccgtg ttggccagca
     2641 caaagggcag ccagagcgtg accaccttgc gcttggcaaa atgtaccagc ccgccccgca
     2701 ggctgaaaag ccacccgctg agcataaaaa acagtgccat gtggaaaaga tagataaaac
     2761 cggtgccggg aaagcccgag tgccccagca ccataagcac gatgccgatg ccgcgcatac
     2821 tatctaatac agggtcgcgc tgctttgcca taagtaccaa cctcgctttt acaaaaatac
     2881 cgcccacggt tgggcggcaa gggtcactgc gcgtttttag cgcggcattt ggggcagata
     2941 taccggatcg tcagcgtgta accgcgtacc tcggtgccga tggcggactc aatatcgcgg
     3001 atcagcccca ccaccggcag gtcggccagg tcgccgcagc tttcgcacag caggtggccg
     3061 tgggggctgg ggttggcgtc gtagcggtcg gggccgtccg gcatttccag gtggccgatc
     3121 tcgccgtcca actccataag ctttaggttg cggtacacgg tgccgcgggc aatgtgcggc
     3181 atggcctgcc gcgcgtggct gaaaatttcg tcagcggtca tatgctcggg gcatttttca
     3241 cgcataatgt ccagtatcag ggcgcgttgg cgggtcatgg gcagacctcc tttaaggatt
     3301 taagattaac tcctattcag atggtacact attttggatg atttgtcaac gttttgggca
     3361 aaatacacaa gagttgttct cgatatttga cttgacatac gcacttctta tggtataata
     3421 gaagaaaatt aagaacaaat cctaatttaa aacggaggta ttcactatgg aactcagagg
     3481 cagcaagacc gagaaaaacc tgtgggaggc tttcgcaggt gagagccagg cccgcaacaa
     3541 gtacacctac tttgcatcca aagccaagaa ggaaggctac gagcagatca gcgccatctt
     3601 caccgagacc gccaacaacg agaaagagca cgccaaaatt tggttcaaac tcctgatgga
     3661 gaacggcgag atccccgaca cgctgacctg cctgaagatg gcctccgcgg gcgagaacga
     3721 ggagcatacc tcgatgtacc cgcgcatggc cgccgaggca aaggaggagg gcttcaacca
     3781 ggttgcagcc ctgttcacga tggtcgccgc catcgagaag gagcacgaag agcgctacaa
     3841 ggccctggcc gccaacatcg aagctggcaa ggtctacgcc cgcgagaccg agcaggtctg
     3901 gcagtgccgc aactgcggtt tcgtctatat cggccagcac gcgccggtca aatgcccggt
     3961 ctgcgcccat ccgcagagct attttgagct gaagagcagc aactactaag agagcagaaa
     4021 agagaagcgc ggtttccaaa atgggaaagc gcttctcttt ttccatggaa gagcttttat
     4081 gagcgatacg gctgggagtt tttgtgcatg gtgcagggcg acggtgagga tgagataacc
     4141 cg
//
LOCUS       DBMDSQ010000181         4124 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_5059_length_4124_cov_2.105891, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000181 DBMDSQ010000000
VERSION     DBMDSQ010000181.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 4124)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4124)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4124
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5059_length_4124_cov_2.105891"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(15..293)
                     /locus_tag="ACZPAX_07075"
     CDS             complement(15..293)
                     /locus_tag="ACZPAX_07075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113699.1"
                     /translation="MCNSLVHYIAAKIKALLSQTVRHAAQSLLALCGVGIIEDGKLCW
                     SPPGYTTICCNFGEPDAYSKMEAANMIASKINLGELPEKKKSPKRKIA"
     gene            complement(518..1306)
                     /locus_tag="ACZPAX_07080"
     CDS             complement(518..1306)
                     /locus_tag="ACZPAX_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015565556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HIR1113700.1"
                     /translation="MKKKLFSLLTALVLCLLCAVPAFAEMPLVMDTYELFKPEATAEL
                     EQKAQDASAGHGVNVYLLTVSDIGGQNVREFAKDWYRNYDLGYGEGKSGILFLIALDS
                     RDYVTITYGGGVTAFTDYRIAQIEDKIVPMLSDGSYYKASDTYIEMCADTLDFYAEKG
                     APLDSGNDPAKGWIKWVFVFVIPLVAAGIVCGIFYAQMKTANEQTHANAYMPAEGLAL
                     SVVEDTYTHTTETETYDPVKVKDDSSSGSSTDSDGFGGSSGGKF"
     gene            complement(1323..2399)
                     /locus_tag="ACZPAX_07085"
     CDS             complement(1323..2399)
                     /locus_tag="ACZPAX_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005930134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HIR1113701.1"
                     /translation="MATQLTNYQCPACTGPLHFDGASGKLVCDFCGSSYDTAEIEALY
                     AEKEAAAETAAQSKQAPPPDSVWSENEAAGLRSYSCPSCGAELICDETTAATSCPYCG
                     NPTVIAGQFSGMQRPELVLPFVLDKNAAKAALKKYYRGKRFLPNAFSSQNHIEEIKGV
                     YVPFWLFDANASGSGLYEATTSSSHRSGDYVITTTKHYDVRRAGTTQFMGVPVDGSTK
                     MPNGHMDAIEPYDYRAFQPFSTAFLPGYMADKYDEDADTCQARAHSRMQNSVSSELSA
                     SITGYDSVSTLGEDINIDYTASHYALLPVWMLHTKWQGKDYLFAMNGQTGKLVGDLPV
                     DKRKVAAWFAGISVPLMILLAFLL"
     gene            complement(2399..2887)
                     /locus_tag="ACZPAX_07090"
     CDS             complement(2399..2887)
                     /locus_tag="ACZPAX_07090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113702.1"
                     /translation="MRKKIAAVLCAAAAFLTMSGCKKAPPGTLTGISISYSGMCYDDT
                     YGFSIRNDPAGGCLFSCNYKGDEWVELENIPVEDTHWQEALALAEKLGLESLPDEKKN
                     SPGLFITDETLNSVCLIYKAPDDEIIYRYLDADGNTRSALRDFLEDLAGQLQTEGKRG
                     DA"
     gene            complement(2887..>4124)
                     /locus_tag="ACZPAX_07095"
     CDS             complement(2887..>4124)
                     /locus_tag="ACZPAX_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005930131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HIR1113703.1"
                     /translation="ADLLATRGWKRQSGRSANTKGSDNIITNGSIVAVADGQCAMIVE
                     QGKVVDMCAEPGEYTYDTGSAPSLFSGDLSDSIGAVFQNIGKRFTFGGEAPMDQRIYY
                     FNTKELVGNKYGTPSPVPFRVVDQRAGIDIDIAIRCFGEYSYRITNPILFYTNVCGNV
                     EEGYTRDEIDGQLKSELMTALQPAFAKISDMGIRYSALPGHTMELAEALNEVLSAKWG
                     KLRGIEIVSFGVSSVKASEEDEQMLKEMQRNAAFMDPTRAAAHLVGAQASAMQTAAGN
                     QGAGPAMAFMGMNMAGAAGGMNAQNLYQMGAQQQAAAQPAPAPAAAPAGWTCSCGQTG
                     NTGKFCANCGSPKPAPTPAAGSWVCSCGTSNTGKFCCNCGNPKPAPAPAKCSQCGWTP
                     VDPAHPPKFCPECGKPFGQ"
BASE COUNT          799 a         1156 c         1250 g          919 t
ORIGIN      
        1 acggagtttt tctcctaagc gatttttcgc ttgggagatt tcttcttttc gggaagttcg
       61 ccaaggttga ttttgctggc aatcatgttc gcggcttcca ttttgctgta ggcatcgggt
      121 tcgccaaaat tgcagcaaat cgtcgtgtaa ccgggcggcg accagcacaa cttgccgtcc
      181 tcgatgatgc cgacgccgca gagggccagc agcgattgcg cggcgtgccg cacggtttgg
      241 gagagcaggg ctttgatttt ggctgcgata taatgaacga ggctgttgca catcccccaa
      301 aaaagaaaga ccgaagtaaa agtccaaaaa gggctttaca acttcggtct ttcgctttaa
      361 gttaacagtt gtgaaaaaag aaactcaaaa gcaagaacag catatcgaaa ttttggaaaa
      421 aggtcaacag ttaaaatttc ggatgccaac agaaataaga atatcgaaac acaacgcgct
      481 ctatcaggca agcatccagc ttgaggaact gtatggttta gaattttccg ccggagctgc
      541 cgccgaagcc gtcgctgtcg gtactcgagc cgctcgagct gtcgtccttg accttgacgg
      601 ggtcgtacgt ttcggtctcg gtggtgtggg tgtaggtgtc ctccaccacg gacagggcca
      661 aaccctcggc gggcatatag gcgttggcgt gggtctgctc gttggcggtc ttcatctggg
      721 cgtagaagat gccgcagacg atgccggctg caaccagcgg gatgacaaac acaaacaccc
      781 acttgatcca acccttggcg gggtcgttgc cgctgtccag cggtgcaccc ttttcggcat
      841 agaaatccag cgtgtcagcg cacatttcta tgtaggtgtc ggaggccttg taatacgatc
      901 cgtccgagag catcggcacg atcttatctt cgatctgcgc gatgcggtag tcggtaaagg
      961 cggtcacgcc gccgccgtag gtgatggtaa cataatcgcg ggaatccagc gcgatcagga
     1021 acaggatacc gcttttgccc tcgccgtagc caaggtcata gttgcggtac cagtccttgg
     1081 cgaactcgcg tacgttctgc ccgccgatgt cagacaccgt cagcaggtag acgttgacac
     1141 cgtgtccggc actggcgtcc tgcgcctttt gttccaattc agcggtcgct tcgggtttga
     1201 acagctcgta ggtgtccatc accagcggca tttcggcaaa cgccggtaca gcgcagagca
     1261 agcacagcac cagtgccgta agcagagaaa acagtttctt tttcatactc tgtttccccc
     1321 tttcaaagca ggaatgcaag caggatcatc agcggaacac tgatgcccgc gaaccaggcg
     1381 gccacctttc gcttatcaac cggcagatcg ccgaccagct tgccggtctg cccattcata
     1441 gcgaacagat agtccttgcc ctgccatttg gtgtgcagca tccagaccgg cagcagtgcg
     1501 tagtgcgagg cggtgtagtc aatgttgatg tcctcgccca gggtgcttac gctgtcgtag
     1561 cctgtaatgc tggccgaaag ctcgctgctg accgaattct gcatacggct gtgggcgcgg
     1621 gcctggcagg tgtcggcgtc ctcgtcgtat ttgtcggcca tatagcccgg caggaatgct
     1681 gtcgagaagg gctggaaggc gcggtagtca tacggctcaa tggcgtccat gtggccgttg
     1741 ggcatcttgg tcgagccgtc caccggaacg cccatgaact gcgtggtacc ggcgcggcgg
     1801 acatcatagt gcttggtcgt ggtgatgacg taatcgccgc tgcggtggct ggagctggtc
     1861 gtggcttcat ataggcccga gccggaggcg ttggcatcga acagccagaa cgggacatac
     1921 acgcccttga tctcctcgat atggttctgc gaggagaagg cattgggcag gaaacgcttc
     1981 ccgcgatagt attttttcag cgcggccttg gcggcgttct tatccagcac aaacggcagg
     2041 acaagctcag gccgctgcat accgctgaac tgccccgcaa tgaccgttgg gttgccgcag
     2101 tacgggcagc ttgtcgcggc ggtcgtctcg tcacagatca gttctgcgcc gcaggacggg
     2161 caggaatagc tgcgcagccc ggcggcctcg ttttcgctcc agacggagtc gggcggcggt
     2221 gcctgtttac tctgtgccgc ggtttcggca gcggcttctt tttcagcata gagcgcttcg
     2281 atctcagcag tatcgtagct gctgccgcag aagtcacaca ccagctttcc gctggcaccg
     2341 tcaaagtgaa gcggaccggt acaggcaggg cattggtagt tggtaagctg cgttgccatt
     2401 atgcgtcacc tctctttcct tcggtttgca gctgccctgc cagatcctcc aaaaaatcac
     2461 gcagggcgct gcgcgtattg ccatcggcat ccagataacg gtagatgatc tcgtcatccg
     2521 gggctttgta aatcaggcaa acgctattta acgtttcatc ggtaataaat aagccaggag
     2581 aatttttctt ttcgtccggc aggctttcca gccccagctt ttctgccaga gccagggctt
     2641 cttgccaatg ggtgtcctca acagggatat tttccagttc cacccattca tcacccttgt
     2701 aattgcagct gaaaaggcag ccgccagccg ggtcgtttct tatgctgaac ccataggtgt
     2761 cgtcatagca cataccggaa taactgatgc tgataccggt cagtgtgccg ggcggtgctt
     2821 ttttacagcc ggacattgtc agaaaagcgg ctgcggcgca aagcaccgca gcaatttttt
     2881 tgcgcatcac tggccaaacg gcttaccgca ctccgggcag aacttgggcg gatgggccgg
     2941 gtcaaccggg gtccaaccac actggctgca cttggcgggg gccggtgctg gcttggggtt
     3001 gccgcagttg cagcagaact taccggtgtt ggaggtgccg cagctgcata cccagctgcc
     3061 ggctgcgggt gtcggggcag gcttggggct gccgcagttg gcgcagaatt ttccggtgtt
     3121 gccggtctgg ccgcagctgc aggtccagcc tgcgggagcg gctgccgggg ccggggcggg
     3181 ctgtgcggcg gcctgctgct gggcgcccat ctggtacagg ttctgggcgt tcataccgcc
     3241 cgccgcaccg gccatattca tgcccataaa ggccatggcg gggcctgcgc cctgattgcc
     3301 ggctgcggtc tgcatggcgc tggcctgtgc gccgacaaga tgcgcagcgg cgcgggtcgg
     3361 gtccataaag gctgcgttgc gctgcatttc cttgagcatc tgctcgtctt cctcgctggc
     3421 cttcacgctg gaaacaccga aggatacgat ctcaatgccg cgcagcttgc cccacttggc
     3481 agagagcact tcgttgaggg cctcggcaag ctccatcgta tggccgggca gtgcggaata
     3541 gcggatgccc atatcgctga tcttggcaaa ggcgggctgc agggcggtca tcagctcgga
     3601 tttcagctgc ccgtcgattt catcgcgggt gtagccttct tccacattgc cgcagacatt
     3661 agtgtagaac aaaatggggt tggtgatgcg gtagctgtac tcaccgaagc agcggatggc
     3721 aatgtcgatg tcgatgccgg cgcgctgatc caccacgcgg aagggcaccg ggctgggcgt
     3781 gccgtatttg ttgcccacca gctccttggt gttgaagtaa tagatgcgct gatccatcgg
     3841 ggcctcgccg ccgaaggtaa agcgcttgcc gatgttctgg aacactgcgc cgatcgagtc
     3901 ggacaggtcg ccgctgaaga gcgagggggc gctgccggta tcgtaggtgt actcacccgg
     3961 ctcggcgcac atatccacga ccttgccctg ctcgacgatc atggcacact ggccgtcggc
     4021 aacggccacg atcgagccgt ttgtaatgat gttgtcgctg ccctttgtgt tggcgctgcg
     4081 tccgctctgg cgtttccagc cgcgggtagc caacaggtca gccg
//
LOCUS       DBMDSQ010000182         4122 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_5065_length_4122_cov_1.346431, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000182 DBMDSQ010000000
VERSION     DBMDSQ010000182.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 4122)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4122)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4122
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5065_length_4122_cov_1.346431"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..762)
                     /locus_tag="ACZPAX_07100"
     CDS             complement(<1..762)
                     /locus_tag="ACZPAX_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017415532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1113704.1"
                     /translation="MPKYLLRRFAEMLITLFLIATATFFLLEAVPGDPLTERASKLSP
                     TVKENLYKRYGLDKPMMERYVITMKGICKGEFGESVVYAGQTVQKLIHDRLPVSARLG
                     IQQVLAGITIGLLLGILAAMRRGSFWDYMVIALSVLLISIPNLIFGLMLQKIFAGNLK
                     VLPTIGWPAGKDLWFGGWEYTILPTLTGCFSYIATYARLLKTSMLDVINQDYVLTAES
                     KGLSRGQIIRRHILRNSFIPVITQLPMSVAMCITGS"
     gene            complement(878..1528)
                     /locus_tag="ACZPAX_07105"
     CDS             complement(878..1528)
                     /locus_tag="ACZPAX_07105"
                     /inference="COORDINATES: protein motif:HMM:NF012706.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR1113705.1"
                     /translation="MPVLLWAFVQRRHAVCRLLRAYGFRGRQYAGDADKLKALFQKGL
                     DELGITDKPEDITITLLSQGSATENQLEREYLQQSISQNLGVKVELNTVGDYQMFANE
                     RDNKNYDIFVGGWFSDYNDPLDFLNIMKTGVYDSYGLYSNSEYDSLIDSLTGENDNAK
                     RLEIYQKLEDLLVAQDCGVAPLYYSDKHYYYQNWVKDFYTSSFGASQELFRATVEK"
     gene            complement(1420..1905)
                     /locus_tag="ACZPAX_07110"
     CDS             complement(1420..1905)
                     /locus_tag="ACZPAX_07110"
                     /inference="COORDINATES: protein motif:HMM:NF012955.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR1113706.1"
                     /translation="MNIQRFHGVPPLYTNTFLIITNAKHGIIIDAAAEPETYLKALDE
                     AGATLTHILQTHGHFDHVGAVASLKKATGCKVYMDKIDAEGSQLLPLTPDVITDEWPA
                     NGELVIDELPFKIYRTPGHTKGGVCLSCYGLLFSGDTLFAGSCGRTDFAGGSMQEMQT
                     S"
     gene            complement(1932..2387)
                     /gene="dtd"
                     /locus_tag="ACZPAX_07115"
     CDS             complement(1932..2387)
                     /gene="dtd"
                     /locus_tag="ACZPAX_07115"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - peptidyl-tRNA hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HIR1113707.1"
                     /translation="MRAIIQRVTRASVTVNGGEPRKIGPGLVILLGVRDTDDSAIVPK
                     LAEKCAHLRIFEDENGKLNRSAVELGYSALVVSNFTLYGDTAKGKRPSFIHAAKGELA
                     VPCYEKFLEEMGHQGLKDVQHGEFGADMKIDLVNDGPVTIVIDTDEWKK"
     gene            complement(2398..>4122)
                     /locus_tag="ACZPAX_07120"
     CDS             complement(2398..>4122)
                     /locus_tag="ACZPAX_07120"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015538323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HIR1113708.1"
                     /translation="HNMRTGDAWPEQKRRDKALETMEVYAPIAHRLGISNIKEELEDR
                     SLHYLDPVGYETIRDLLNKHGDEFLHQVCHTIAKHLAENGIQKATIRHRVKSIYGIYR
                     KMYMQNKDFEEIYDIYAVRIILDNVPECYTALGLIHDMYHPLPNRFKDYISTPKPNGY
                     QSLHTTVIGREAIPFEVQIRTWDMDRMAEYGIAAHWKYKAGITGGNDKLDERLAWVRQ
                     LLESQRSSADAADLLSDIKSDLLPEEVFAFTPRGDVINLPAGATAIDFAYAIHSAVGN
                     RMIGAKVNNRIVPIDHKVQTGEIIEIITGPENRGPSRDWLNIVKTSEAKNKIRNWFKK
                     ERREENIQEGRDALEREMRRNLMTLTDEQHDAFMEALARRNRCNSVEEMYAAIGYGGL
                     QISRMLPKLKEEYTKLQATEPKPVAVELKRMHSSDGVIVEGIDNCPIKFAKCCSPLPG
                     DEIIGFVTRGFGVSIHKKDCANARESMRHPENADRWVRAYWDEAEKENYKATLDIICM
                     DRPNLVSDVALALGDMRVPIYSLMARAAEQGRARMSVTVGITNTEHLNSVVARLKKIK
                     DVVSVTRN"
BASE COUNT          819 a         1119 c         1164 g         1020 t
ORIGIN      
        1 ggagcctgtg atacacatgg caaccgacat cggaagctgt gtgataaccg ggataaacga
       61 gttccgaagg atatggcggc ggataatctg tccgcggctt aagcccttcg attcagcagt
      121 cagcacgtaa tcctgattga tcacatcgag catcgaggtt ttcagcagac gcgcatacgt
      181 tgcgatataa gaaaagcatc ctgtcagggt cggaagaatc gtgtattccc atcctccgaa
      241 ccacagatcc tttcccgcag gccagccgat cgtcggcaga accttcaggt tacctgcgaa
      301 aattttctga agcatcagac caaagatcag gttcggaatg gaaatcaaga ggaccgacag
      361 ggcgatcacc atatagtccc agaagcttcc cctcctcatt gccgcaagaa tcccaagcag
      421 cagaccgatc gtgataccgg ccagcacctg ctggatacca aggcgggcgg ataccggaag
      481 acggtcatga atcagcttct gtaccgtctg tcccgcatag acaacagatt ccccgaactc
      541 tcccttgcag attcccttca ttgtgatcac atagcgctcc atcatcggct tgtcgaggcc
      601 gtagcgctta tagaggtttt cctttacggt cggcgagagc ttgctcgcac gctccgtcag
      661 cggatctccc ggaaccgctt ccagaaggaa aaacgtggcc gttgcaatca gaaacagtgt
      721 gatcagcatt tccgcgaaac ggcgcagtaa atattttggc ataaaaatcc tctccctgtc
      781 cggtcttttc cgaccggaat tttattatga tagcgaaaga agagcctatt gtttcataag
      841 cccttcttcc ctcatttctt ctgttttgct gcttttttta tttctctaca gttgctctga
      901 aaagctcctg gcttgcgccg aaggaggatg tgtagaaatc ttttacccag ttctgataat
      961 agtaatgttt atcagagtag tacagcggtg caacaccaca atcctgtgca accagcagat
     1021 cttccagctt ctggtaaatc tcaaggcgtt ttgcgttgtc gttctctccg gtcagagaat
     1081 cgatcaggct gtcgtactcg ctgttgctat agaggccgta ggaatcataa acgcctgtct
     1141 tcatgatgtt caggaaatcc agcggatcgt tgtaatcgct gaaccatccg cctacaaaga
     1201 tatcgtagtt cttgttatcg cgctcgtttg caaacatctg gtaatcgcca acggtattca
     1261 gctctacctt tacgcccaga ttctgggaaa tggactgctg caggtactct ctctcgagct
     1321 gattctcggt agcagaaccc tgggacagca gtgtaatggt gatgtcttcc ggtttgtcgg
     1381 tgataccaag ctcatcaagg cctttctgga acagtgcttt taacttgtct gcatctcctg
     1441 catactgccg cccgcgaaat ccgtacgccc gcaggagcct gcaaacagcg tgtcgccgct
     1501 gaacaaaagc ccatagcagg acaggcagac gccgcctttg gtatgcccgg gcgtgcggta
     1561 gattttaaac ggtaattcgt caatcacaag ttcgccgttg gcgggccatt cgtcggtgat
     1621 gacgtcgggc gtcagcggca gaagctggct gccctcggcg tcgatcttat ccatatagac
     1681 tttgcagccg gtcgcctttt tcagcgaggc caccgccccc acatggtcaa agtggccgtg
     1741 ggtctgcagg atatgcgtta atgttgcacc cgcctcgtcc aaggctttca ggtaggtttc
     1801 cggctcggcg gcggcatcaa tgatgatgcc gtgctttgca ttggtgatga tcaaaaaggt
     1861 gttggtgtac agcggcggaa cgccgtggaa gcgctggata ttcattgcat cctccccttg
     1921 ggtttgtggg tttatttctt ccattcgtcg gtgtcgatga cgatggtgac tgggccgtcg
     1981 ttgacaaggt caattttcat gtccgcaccg aactcgccgt gctggacgtc tttgaggccc
     2041 tgatgaccca tctcctccag gaatttttca tagcacggca cggccagctc gcccttggcg
     2101 gcatggataa agctggggcg ctttcccttg gccgtatcac cgtagagtgt gaagttggac
     2161 acgacgaggg ccgagtagcc aagctccacg gcgctgcggt tcagcttgcc gttttcgtcc
     2221 tcaaaaatac gcagatgggc acatttttcg gccagcttgg gcacgatggc gctatcgtcg
     2281 gtgtcccgca cacccagcag gatgaccagg cccggcccga ttttgcgcgg ttcaccgccg
     2341 ttgacggtga cactggcacg ggtaacgcgt tggatgatgg ctcgcataat tgtttcctca
     2401 gtttctcgtc acactgacga cgtctttgat tttcttcaaa cgggcaacga cgctgttcag
     2461 gtgttccgtg ttggtgatgc ccacggtcac gctcatgcga gcgcggccct gctcggcggc
     2521 gcgggccatc aaagaataga tgggcacccg catatccccc agcgccagag ccacgtcgga
     2581 cacaaggttc gggcgatcca tacagatgat atccagcgta gctttgtagt tttccttttc
     2641 tgcctcatcc caataagcgc gaacccagcg gtcggcgttc tcggggtggc gcatactctc
     2701 gcgggcgttg gcacagtcct ttttgtggat ggagacacca aagccgcggg tgacaaaacc
     2761 gatgatctca tccccgggca gcggtgaaca gcacttggca aacttgatgg gacagttgtc
     2821 gatgccctct acaataacgc cgtcggagga gtgcatccgc ttgagttcca ccgcgaccgg
     2881 cttcggctcg gtggcctgca gcttggtgta ctcctctttc agttttggca gcatgcggga
     2941 aatctgcaga ccgccgtagc cgatggccgc gtacatttcc tcaacgctgt tgcagcggtt
     3001 gcggcgggcc aatgcctcca taaaggcgtc gtgctgctca tcggtcaggg tcatgaggtt
     3061 gcggcgcatt tcgcgttcca gtgcgtcgcg gccctcctga atattctctt ccctgcgttc
     3121 ttttttgaac cagttgcgaa tcttgttttt ggcctcgctg gtcttgacga tgttcagcca
     3181 gtcgcggctg gggccgcggt tttcggggcc ggtgatgatt tcaataattt cgccggtctg
     3241 gaccttgtgg tcgatgggca cgatgcggtt gttgactttc gcgccgatca tccggttgcc
     3301 gacggcggag tggatggcat aggcaaagtc gatagccgtt gcgcctgcgg gcaggttgat
     3361 gacgtcgccg cgcggcgtga aggcgaacac ttcctcgggg agcaggtcgc tcttgatatc
     3421 gctgagcagg tcggcggcat cggcgctgga acgctggctt tccaaaagct ggcgcaccca
     3481 ggccagacgc tcgtccagct tgtcattgcc gccggtgatg ccggccttgt acttccagtg
     3541 ggcagcgatg ccgtactcgg ccatgcgatc catgtcccag gtgcggatct gcacctcaaa
     3601 ggggatggcc tctcggccga tgaccgtggt gtgcagggat tggtagccgt ttggttttgg
     3661 cgtagagatg taatctttga agcggttggg cagcgggtgg tacatatcat ggataaggcc
     3721 cagtgcagtg tagcactcgg ggacgttgtc cagaatgatg cgcacggcgt agatatcgta
     3781 gatttcctcg aaatccttat tctgcatata catcttgcgg tagatgccgt agatgctctt
     3841 gacgcggtgg cggatggtgg ccttctggat accgttctcg gccaggtgct tggcaatggt
     3901 gtggcagacc tggtgcagga actcgtcgcc gtgcttattt aaaaggtcgc gaatcgtctc
     3961 atagcccacc gggtccagat aatgcaggct gcggtcctcg agttcctcct tgatgttgga
     4021 aatacccaga cggtgagcga tgggcgcgta gacctccatc gtttccaggg ccttgtcgcg
     4081 gcgcttctgc tcgggccagg cgtcgccggt gcgcatattg tg
//
LOCUS       DBMDSQ010000183         4111 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_5078_length_4111_cov_2.268828, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000183 DBMDSQ010000000
VERSION     DBMDSQ010000183.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 4111)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4111)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4111
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5078_length_4111_cov_2.268828"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..698)
                     /locus_tag="ACZPAX_07125"
     CDS             complement(<1..698)
                     /locus_tag="ACZPAX_07125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113709.1"
                     /translation="MSDQKKTSGESFAGNVMKYSVATYLGFAITGLALIVKGLLPAEV
                     LGAPVTFMTYTATLMNIGILGLDQALLRFYHEPPAGAEPRQLFAACTRISASFMVLLG
                     VAGSVFFAQPLAAAFGLGSAGPGIVPLLFINAALYMLVRYLNVLLRLENDLRAYTAET
                     LWMQGCYNLFYLLPGFFTSNVFILAVAAILSFGVVAIAFGYKYRSAVFAPLPASQLGG
                     IARQTVPYGVALAPA"
     gene            complement(813..2126)
                     /locus_tag="ACZPAX_07130"
     CDS             complement(813..2126)
                     /locus_tag="ACZPAX_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007048234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="surface carbohydrate biosynthesis protein"
                     /protein_id="HIR1113710.1"
                     /translation="MALDFLILYEHTVREYESDLLLKLELERRGYTVEIRQLLDPKHL
                     RLFGKDKPEVLVASCMYDNEAINSHVYNNIGKCNKIVNLHWEQMLSDTQEEGDWFNMN
                     GNAKRCVQTCWGKRTAARLQAHGMDAKNTPVTGAVMMDFLRPEFDGYFKDKQTLCKEF
                     GLDPAKQLHLYISSFGYASMNDDEVAELSKMAGTDFTGFAKTNRVSMKETLRWFDEYL
                     GQHPEVELVYRRHPSEWNSPALEELAKKRPNFHVIFADSVKQWIVAADSISIWMSTAI
                     AEVYMAGKSCHILRPVPIEHEYDPVIYKDAHYVTTCGEFAAAMAAPEPPFPIAKDVIE
                     GYFDPSDTPAYKRMADLLEDVYKNPPRDEPMGEGFTPHFNKVKFCALAGVHFLYRHGW
                     EPKKVFAFCPPLANFAQRIYGYVDKAHVTPEQVAAMTERIKPFVR"
     gene            complement(2292..3236)
                     /locus_tag="ACZPAX_07135"
     CDS             complement(2292..3236)
                     /locus_tag="ACZPAX_07135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113711.1"
                     /translation="MKNILKKLALLMIPVLLWFAFFVAFEPNNYFGLKVSASSSQPVA
                     RVRAYQQESGTNLILGDSRLAHFDMALVDELSGRNWQNLAFGGASLKETLDLADYVLD
                     SGREVDTLLVEVSFYTLNAGYNTDRFATLEKTLNNPLAYCFNLEYNVNALTVAMDTLR
                     GTPDTIESGDWTDADYLADDGTVLPLHRKLYDYTATITPRCKSWALNTEQFDRLHTLA
                     RRCQAEGVRLIVVLPPMADNVRTEVCDVFGITDVMQDTVLPQLTGWAAECGFTLLDYE
                     WGGSAITDDDTQFFDGFHLDEKYGLPVWTQELFNDIAG"
     gene            complement(3461..>4111)
                     /locus_tag="ACZPAX_07140"
     CDS             complement(3461..>4111)
                     /locus_tag="ACZPAX_07140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113712.1"
                     /translation="PLAWADMSGVTGGRLTRLPAEVCVFTVFFVSGFWHGNTLPFVVW
                     GLLQACYRLGEELLHRRLGKPKKKAPARVLWAKRAGVFVLWCISMVFFRVGSAPAAAP
                     LTVGDAFAYLGRCFLGWSPARFGSELYAAASNGFYANAIMVAAYYVFVVLVLALAFYL
                     DTRRAFAYKNKPAEVCLAGEKHRWAVYYALVVALLVGYIMQSGGFGNSGFGMYAGF"
BASE COUNT          849 a         1266 c         1225 g          771 t
ORIGIN      
        1 gccggggcca gtgccacgcc gtagggcaca gtctggcggg caatgccgcc cagctggctg
       61 gcgggcagcg gcgcaaacac agcgctgcgg tacttgtacc cgaatgcaat ggcaaccacg
      121 ccaaagctta aaatggccgc cacggccagg ataaatacgt tggaggtgaa aaagcccggc
      181 agcaggtaga acagattgta gcagccctgc atccagagtg tttcagccgt gtaggcgcgc
      241 aggtcgtttt ccaggcgcag cagcacgttc agataccgca ccagcatata cagcgccgca
      301 ttgatgaaca gcagcggcac gatgccgggg cctgccgacc ccaggccgaa ggccgccgcc
      361 agcggctggg caaagaacac gctgcctgcg acacccagca gcaccatgaa cgacgccgaa
      421 atgcgggtgc aggctgcgaa cagctggcgc ggctcggccc cggcgggcgg ctcatggtaa
      481 aagcgcagca gcgcctgatc caggcccagg atgccgatgt tcatgagggt ggccgtgtag
      541 gtcatgaagg tgaccggcgc acccaggacc tcggcgggca gcagcccctt gacgatgagc
      601 gccagcccgg tgatggcgaa gcctaaatag gtcgccaccg agtatttcat cacatttccg
      661 gcaaaggatt caccggaggt ttttttctgg tctgacatga ttatttgtcc tgtcgtagag
      721 ttgtaagggc cgggcatgcc cggcccgcgg ccttgccatt actgcgcgtc tgccgcactg
      781 cggagggccg ggcatacccg gcccctacat gctcagcgga cgaacggctt aatgcgttcc
      841 gtcattgctg ccacctgttc cggcgttaca tgggctttgt ccacataccc ataaatgcgc
      901 tgggcaaaat tggccagcgg ggggcagaag gcaaacacct ttttcggctc ccagccgtgg
      961 cggtagagga aatgcacccc ggccagcgca cagaacttta ccttattaaa atgcggcgtg
     1021 aagccttcgc ccattggctc gtcccggggc gggttcttgt atacatcctc caataggtcg
     1081 gccatccgct tgtaggcggg cgtatcgctc gggtcaaaat agccctcgat aacgtccttt
     1141 gcaatcggga acggcggttc cggcgcggcc atggcggcgg caaactcgcc gcaggtcgtc
     1201 acataatggg catccttgta gatgaccggg tcgtactcgt gctcaatggg caccgggcgc
     1261 aggatgtggc agctcttgcc ggccatatac acctcggcga tggcggtgct catccagatg
     1321 gaaatagagt ctgccgccac gatccactgc ttgaccgagt cggcaaaaat gacatggaag
     1381 ttcggccgct tcttcgccag ttcttccagc gcggggctgt tccactcgct ggggtggcgg
     1441 cggtagacca gctcgacctc gggatgctga cctaaatatt catcgaacca acgcagtgtc
     1501 tccttcatcg agacacggtt cgtcttggca aagccggtaa aatcggtgcc cgccatcttg
     1561 gaaagctcgg cgacctcgtc gtcattcatg ctggcgtagc cgaagctgga aatatacagg
     1621 tgcagctgct tggccgggtc caggccgaac tctttgcaca aggtctgttt gtctttaaag
     1681 tagccgtcaa actccggccg cagaaagtcc atcatgactg cgccggtgac aggcgtgttt
     1741 ttggcgtcca tgccgtgggc ctgcaggcgg gcggcggtgc gcttgcccca acaggtctgt
     1801 acgcagcgct tggcgttgcc gttcatattg aaccagtcgc cctcctcctg cgtgtcggaa
     1861 agcatctgct cccaatgcag gttgacgatc ttgttgcatt tgccgatgtt gttgtagaca
     1921 tggctgttga tggcctcgtt gtcatacata cagctggcga cgagcacttc cggcttgtcc
     1981 ttaccgaaca gccgcaggtg cttcgggtcc agcagctggc ggatctccac cgtgtaacca
     2041 cggcgttcca gctcgagctt caagagcagg tcgctctcat actcgcggac ggtatgctcg
     2101 tataaaatca ggaaatctag tgccattgat agcaactccc atgcaaagta agttgtaggg
     2161 gcgggtgctt gcatccgctc gtggggcgtc ggggacgccg ccccctacaa atgaggatgc
     2221 ccctttgtcc ggtggatgta gggcggggtg ccctcacccc gcggcggcat gggggcatgc
     2281 cgccctacat gtcaacctgc aatatcgtta aacaactctt gtgtccaaac cggcaggccg
     2341 tacttttcat ccagatggaa accgtcaaaa aactgcgtgt catcgtcggt gatagcgctg
     2401 ccgccccact cgtaatccaa tagggtaaag ccgcattctg cggcccagcc ggtcagctgc
     2461 ggcagcacgg tatcctgcat cacatcggtg atgccgaaca cgtcacagac ctcggtgcgg
     2521 acattatcag ccatcggagg cagaaccacg atgagccgca caccctcggc ctggcagcgc
     2581 cgggccagcg tgtgcaggcg gtcgaattgc tcagtgttca gcgcccagct tttgcagcgg
     2641 ggcgtgatgg tggcggtgta gtcatataat ttgcggtgca gcggcaaaac cgtgccatcg
     2701 tcggccaggt aatccgcgtc ggtccagtcc ccggactcga tggtgtccgg cgtgccgcgc
     2761 agcgtatcca tggccacggt cagggcattg acgttgtact ccaaattgaa gcagtaggcc
     2821 agcggattgt tcagcgtctt ttccagcgtg gcaaagcggt cggtgttgta cccggcgttc
     2881 agcgtgtaga acgagacttc caccagcagg gtgtcaactt cgcggccgga atccagcaca
     2941 tagtccgcga ggtcgagggt ttctttcagc gacgcgccgc cgaaggcgag attctgccaa
     3001 ttccggccgg aaagctcgtc caccagcgcc atatcaaaat gcgccaggcg gctgtcaccc
     3061 aagatcagat tcgtgccaga ctcctgctgg taggccctta cccgcgccac cggctggctg
     3121 gacgaagcgg agactttcag cccgaagtag ttgttaggct caaaggcaac gaagaatgcg
     3181 aaccaaagca aaactggaat catcaataat gcaagttttt ttaggatatt cttcatgggc
     3241 ggaagcctcc tttcgttaaa atggcttccc cctcggggga agctgtcagc ggcttgtccg
     3301 ctgactgatg aggggcgagt ttgccgcggc gacccattca tgggcaatcg cggtacatac
     3361 gccccgcacc cggcgctgcg gcgccacctg ctccccctct ggcgctacgc gccacctccc
     3421 ccgtatcggg ggagtctgtc ccttggggga aggcgtgggg ttaaaacccc gcatacatac
     3481 cgaatccgct gttcccaaat ccaccgctct gcatgatgta ccccaccagc aaagcgacga
     3541 ccagggcata atacacggcc cagcggtgct tctcgcccgc caaacacacc tcggcgggct
     3601 tgttcttata ggcaaacgcc cggcgggtgt cgaggtagaa cgccagcgcc agcaccagca
     3661 ccacaaagac atagtacgcc gccaccataa tggcgttggc gtagaatcca ttagacgcag
     3721 cagcgtacaa ttcgctgcca aaccgcgcgg gtgaccagcc caggaagcag cggcccaggt
     3781 aggcaaacgc atcccccacg gtcagcggcg cggcggcggg ggcggacccc acgcggaaga
     3841 acaccatgct gatgcaccac agcacaaaca ccccggcccg cttggcccac aaaacgcggg
     3901 cgggcgcttt ctttttgggc ttgcccaggc ggcggtgcaa aagctcctcg cccaggcggt
     3961 agcaggcctg cagcagaccc caaaccacaa agggcagcgt gttcccatgc cagaaacccg
     4021 acacaaaaaa caccgtaaac acgcacacct cggcgggcag gcgggtcagc ctgcccccgg
     4081 tcacgccgga catatccgcc cacgccagcg g
//
LOCUS       DBMDSQ010000184         4109 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_5081_length_4109_cov_1.734032, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000184 DBMDSQ010000000
VERSION     DBMDSQ010000184.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 4109)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4109)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4109
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5081_length_4109_cov_1.734032"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            4..84
                     /locus_tag="ACZPAX_07145"
     CDS             4..84
                     /locus_tag="ACZPAX_07145"
                     /inference="COORDINATES: protein motif:HMM:NF036802.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113713.1"
                     /translation="MREALSSYIYRRTKRSPMILPILLEV"
     gene            110..2077
                     /locus_tag="ACZPAX_07150"
     CDS             110..2077
                     /locus_tag="ACZPAX_07150"
                     /inference="COORDINATES: protein motif:HMM:NF013528.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HIR1113714.1"
                     /translation="MKHKDGLDIAAVLLLLLAAVAVMVVPVALVRPQLLAVPAMLVLI
                     ALCVVLYQRRRLRTFLAKQLCSTEFENSRIQYSLANLPIATVLINDGRILWYNQHFKE
                     QVLGDYDAVTRPVERVLPEFDLAVCSRPHGQDLTVGERRFTAYSATAKGSRGASLVYL
                     IDDTFYKETLDEYNASRPACLIIVIDSYDELFDDMKDSEQAKELEAINSLLEEYIGRS
                     SGFLRKISNSRYIAVVEERDVRWMLEERFDILDKVRALHPGGLTTLSIGVGRGGKNLQ
                     ECHQMARESIDIALGRGGDQAAVKTVDGFEFYGGISHGVEKRSHVRSRIISNALCDLI
                     KRSDSVIIMGHRMGDLDAVGSAVGVLRMCKMCGVPAVIAVNSEATLAGPLLKTFIEAG
                     EGHNFIAPDQTLDVITPNTLLIIVDTYQKRLLESQQIYERCKRVVVIDHHRMAVGHID
                     NPLLLYHEPYASSASELVCELLQFMPKENNITPLEAQALLAGIMLDTRSFALHVGVRT
                     FEAAAWLRSRGAQTADTKLLFNTSKEEYEARAHIVEAAYIYKGCAIALSGELEAGMNV
                     VLPMAANDLLTINGVDASFVAVAKNGGVNISARSMGALNVQVILEPLGGGGHLMMAGA
                     QLQNCTLQDAEHRIREQIDLYRAAQAKNAAQ"
     gene            2108..2554
                     /gene="rplI"
                     /locus_tag="ACZPAX_07155"
     CDS             2108..2554
                     /gene="rplI"
                     /locus_tag="ACZPAX_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HIR1113715.1"
                     /translation="MKIILKQDVKTIGKKDEIHEVSDGYARNFLFPRDLAAPADAAAL
                     NQARTKSEAKAHHEAEARAAAEALAAKIKGQVITAKVKGGASGKLYGKVTGKEVAELL
                     TTLTGTEIEKKKVELPSAIKEFGSYDASVRLYAGVVAPFKLKVEEL"
     gene            2633..3982
                     /gene="dnaB"
                     /locus_tag="ACZPAX_07160"
     CDS             2633..3982
                     /gene="dnaB"
                     /locus_tag="ACZPAX_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HIR1113716.1"
                     /translation="MPYNMQAEQSVLGAALMDETVLNRLITDMEPEMFYSDQNRAVYE
                     TMRSLYTESEAVDVITLVNALANGGTFASADDAKVYITRIAETVPAISNVDSYFKIVK
                     EKYQARRLIDAARGILSETASGEDADLLLESAEQKIYDIRSGRDKTGVKTIKESILEV
                     IDTMQKLSGADRDKFAGIPTGFNYLDTVLTGLGRSDLIILAARPGMGKTSFALNIATN
                     VARQQKVPTIIFSLEMTCEQLTDRILSSTANIDSQAFRTGRLNNSDWNDFAQATSMLY
                     NAPIYMDDSSGISVPEIKAKIRTINQDPKKEKIGLVIIDYLQLMQSAKRTESRVQEIS
                     DITRNLKIMAKELNVPVIALSQLSRAAEKTAGRSDHRPQLSDLRDSGSIEQDADIVLF
                     LYRAAYYNSQNGEDSQANENEAECIVAKNRHGETSVVRLGWDGAHTRFSNLDVAHEF"
BASE COUNT          920 a         1234 c         1170 g          785 t
ORIGIN      
        1 cgcgtgcggg aggcgctgtc cagctatatc taccgccgca ccaagcgcag cccgatgatt
       61 ctgccgatcc tgctggaagt gtaaaagcct ttgagctgag gaatgtacaa tgaaacacaa
      121 ggatggtctg gatatagccg ccgtactgct gctgttgctg gccgccgtcg ccgttatggt
      181 cgtgccggtg gccctcgtca ggccgcagct gctcgctgtg cctgcgatgc tggtgctcat
      241 tgcactttgc gtggtgctct accagcgccg ccgtctgcgc acctttctgg ctaaacagct
      301 gtgcagcacc gagtttgaaa acagccgtat ccagtacagc cttgccaacc tgcctattgc
      361 gaccgttctt atcaacgatg gccgcatcct gtggtacaac cagcatttca aagagcaggt
      421 cctcggcgat tatgacgccg tgacccgccc ggtagagcgc gttctgccag agtttgacct
      481 ggctgtctgc tcccgccccc atgggcagga tctgaccgtg ggggaacggc gcttcaccgc
      541 ctactctgcc accgccaagg gcagccgtgg agccagcctt gtgtacctta tcgacgatac
      601 cttctataag gaaacactcg atgagtacaa cgccagccgc ccggcgtgtc ttatcatcgt
      661 tatcgacagc tatgatgagt tgttcgatga tatgaaggac agtgagcagg ccaaggaact
      721 ggaagctatc aacagcctgc tggaagagta cattggccgc agctcgggct tcctgcgcaa
      781 aatctccaac agccgctata ttgcggtggt ggaggagcgc gacgtgcgct ggatgctgga
      841 agaacggttc gatatcctcg ataaagtccg tgccctacac ccgggcggtt tgaccacact
      901 gtccatcggc gtaggccgcg gcggcaagaa cttacaggaa tgccatcaga tggcccgcga
      961 gagcattgac attgcccttg gccgcggcgg agaccaggcc gccgtaaaaa ccgtggacgg
     1021 ctttgagttc tacggcggca tttcccacgg cgtggagaag cgcagccacg tccgcagccg
     1081 cattatctcc aacgcgctgt gcgaccttat caagcgcagc gacagcgtca tcattatggg
     1141 ccaccgtatg ggcgacctgg atgccgtcgg ctcggcggtc ggtgtgctgc ggatgtgcaa
     1201 gatgtgcggt gtgcccgccg tcattgccgt taacagcgag gccacgctgg cagggccact
     1261 gctgaaaacg tttatcgaag caggtgaggg acacaacttt atcgcccctg accaaacgct
     1321 ggacgtcatc acgccgaaca ccttgttgat catcgtcgat acctaccaga agcgactgct
     1381 ggaaagccag caaatctacg aaagatgcaa gcgcgtcgtc gtcatcgacc atcaccgcat
     1441 ggcggtgggg cacatcgaca atccgctgtt gctctaccac gaaccctatg cgtccagcgc
     1501 tagcgagctg gtctgtgaac ttttgcaatt tatgccgaag gaaaacaaca tcaccccgct
     1561 ggaagcccag gctctgctgg ccggcatcat gctggacacc cgcagctttg cactgcatgt
     1621 cggcgtgcgt acctttgaag ctgctgcctg gctgcgcagc cgcggtgccc agactgccga
     1681 caccaagttg ctatttaaca cctcgaagga agagtatgag gcccgcgccc atatcgtgga
     1741 ggcggcctac atatataagg gctgtgcaat cgccctgtct ggcgaactgg aagccggtat
     1801 gaacgtggtg ctgcccatgg cggctaacga cctgctgacc atcaacggtg tggacgccag
     1861 ctttgtggcc gtcgccaaga acggcggtgt caatatctca gcccgcagca tgggcgcact
     1921 gaacgtacag gtcattctgg aaccgctggg tggtggcggc catctgatga tggcgggcgc
     1981 acagctgcag aactgcaccc tgcaggatgc ggaacaccgc atccgtgaac aaatcgacct
     2041 ttaccgcgcg gcgcaagcca aaaacgccgc acagtaatcg ggatataatt ttgggaggga
     2101 aacccttatg aagatcattc tcaaacagga cgtcaagacc atcggcaaga aagatgagat
     2161 ccatgaagtc tcggacggtt acgcccgcaa cttcctgttc ccgcgcgacc tggcagcccc
     2221 ggcagatgcc gctgccctga accaggcccg caccaagagc gaggccaagg cccaccacga
     2281 ggccgaggcc cgtgccgccg ccgaagccct ggctgccaaa atcaagggcc aggtcatcac
     2341 cgctaaagtc aagggtggtg cctccggtaa gctgtacggc aaagtcaccg gcaaggaagt
     2401 cgccgagctg ctgaccaccc tgaccggcac cgaaatcgaa aagaaaaagg tcgagcttcc
     2461 ttccgctatc aaggagttcg gcagctatga tgcctccgtg cgcctgtacg ccggtgttgt
     2521 tgcgcccttc aagctgaagg tcgaggaact gtaactacaa atttaacagg ggaggaggac
     2581 gcacgctgtg ccaaaaaatg aagaagtaag cctgtccagc ctgggtatcc agatgcccta
     2641 caatatgcag gcagagcaga gcgtgctggg ggctgcgctg atggatgaga cggtgctgaa
     2701 ccggctcatc accgacatgg aacccgaaat gttctactca gaccagaacc gcgctgtcta
     2761 cgagacaatg cgttcgctct ataccgagag cgaggccgtt gatgtcatca cgctggtgaa
     2821 cgcccttgcc aacggcggta cctttgccag cgctgacgac gctaaagtgt acattacccg
     2881 cattgccgag accgtccctg ctatctccaa tgtcgattct tattttaaga tcgtcaagga
     2941 gaagtaccag gcccgtcgcc tcatcgatgc cgcccgcggc attttgagcg agacggcctc
     3001 cggcgaggac gccgacctgc tgttggaaag cgctgagcag aaaatctacg atatccgctc
     3061 gggccgtgat aagactggcg tcaagaccat caaggagtcc atccttgagg tcattgacac
     3121 gatgcagaag ttaagcggtg ctgaccgcga caagtttgcc ggcattccca cgggcttcaa
     3181 ctacctggac acagtgctca ccggcctggg ccgctcggat cttatcattc tggccgcccg
     3241 ccccggtatg ggcaaaacgt cctttgcgct gaatatcgcc acgaacgttg cccgccagca
     3301 gaaggtgccc accatcattt tcagcctgga aatgacctgc gagcagctga ccgaccgcat
     3361 cctttccagc acagcgaaca tcgacagcca ggcgttccgc accggccgtc tgaacaactc
     3421 cgactggaac gactttgccc aggcgacctc gatgctctac aatgccccca tttacatgga
     3481 cgattcctcg ggtatctcgg tgccggagat caaagcaaag atccgcacca tcaaccagga
     3541 tcccaagaaa gaaaagatcg ggctggtcat catcgactac ctgcagctga tgcagagcgc
     3601 caagcgcacc gagtcccgcg tgcaggaaat cagtgatatc acccgaaact taaaaatcat
     3661 ggcaaaggaa ctcaacgtcc ctgttatcgc gctgtcccag ctgtcacgtg cggcggaaaa
     3721 aacggcaggc cgctccgacc accgccccca gctgtccgac ctgcgtgact ccggctctat
     3781 cgagcaggac gccgatatcg tgttgttcct ctaccgcgcg gcctactata actcccagaa
     3841 cggtgaggac agccaggcca acgagaacga agccgagtgc attgtggcca agaaccgcca
     3901 cggcgagacg agcgtcgtcc gcctgggctg ggatggtgcc cacacgcgct tcagcaatct
     3961 ggatgtagcc catgagttct gacgcggaaa aaactgtaca gcgtattgaa gaggctctgt
     4021 tggacggctg ccgccggcag ggtttgttca agtctggcga cagagtcatt gcggcctgct
     4081 ccggtggggc agactcgatg gcgctgttg
//
LOCUS       DBMDSQ010000185         4105 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_5088_length_4105_cov_1.573177, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000185 DBMDSQ010000000
VERSION     DBMDSQ010000185.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 4105)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4105)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4105
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5088_length_4105_cov_1.573177"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..649
                     /gene="hypB"
                     /locus_tag="ACZPAX_07165"
     CDS             <1..649
                     /gene="hypB"
                     /locus_tag="ACZPAX_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009004363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HIR1113717.1"
                     /translation="EIKQSVFANNDQRAELLRRELKEKRIFLLNLMSAPGSGKTTTLR
                     RTIAALKDELRIGVMEADIDSDVDARAIAGTGAKSIQLHTGGMCHLDAEMTRQGLEGL
                     GTGDIDLAILENVGNLVCPAEFDTGAVKNAMILSVPEGHDKPLKYPLMFQVCDIVLVN
                     KIDVMPYFDFDLDKCREYVAMRNPKAKVIPICAKTGEGIDEWADWLREQVREWQR"
     gene            832..3540
                     /locus_tag="ACZPAX_07170"
     CDS             832..3540
                     /locus_tag="ACZPAX_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004602088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR1113718.1"
                     /translation="MSKPTAFPLDESKLPFRIPKDQPFRENIAKLGQMITDRIPAKKG
                     TLTQDDPEYWGLASIVTDEMAEVALKMKVRKPMTLPELVKATGKPADELEPLLQQMAV
                     VGLLEYNWENPRREKQYILPMFVPGSAEFFNMNKQQIADHPEVTAFFERMTFLPLEHI
                     TAMVPPGGAGIGMHVIPVERAIETENQSVDVEHISHWLKKYDGKYAAGPCSCRMSRAA
                     MGEGCGDDPDDWCIGVGDMADYLVETNKGHYVTYDEVMQILQKAEDNGFVHQITNIDG
                     ENKIFAICNCNVNVCNALRTSQLFNTPNMSRSAYVAKVAPENCVACGRCVEYCPAGAV
                     KLGQKLCTKEGRVVYPRQELPNTVKWGPEKWAIDYRDKNRINCYDTGTAPCKTACPAH
                     IAVQGYLKMAAQGRYRDALALIKKENPFPAVCGRICNRRCEDACTRGTVDQAVAIDAV
                     KKFIAQQDLHAEHRYVPDVVQPSLQGPWPQKIAIIGGGPAGLSCAYFLATQGYKPTVF
                     EKNERPGGMLRYGIPSFKLEKDVIDAEIDVLRELGVDIRCGVDVGRDVTLAQLREQGY
                     KAFYIAIGCQGGRKAGVPGEDAAGIETAVHLLRTVGGDESHKMQGKTVVIGGGNVAID
                     AARVALRCGSDDVTMVCLEPREKMPASVEEIAEAEEEGTKITCGYGPKEFIAENGHVT
                     AVVLKKCTGLYNAEGRFAPTYDENATITLPCENVVLSIGQCIEWGDLLNGEAVELGRG
                     QGAVADALTYQTAQPDVFVGGDVYTGPRFAIDAIAAGKQGAISIHRYVQPNTSMTIGR
                     NRRDFHELDKNNLSLGEYDHAPRQTAALDESFPEVIRACARESAQTERELWARREANA
                     EQNMRKRGICVTELDEAEKARFRAAVQPMYDAFSAQQELIDRIQKS"
     gene            3826..>4105
                     /locus_tag="ACZPAX_07175"
     CDS             3826..>4105
                     /locus_tag="ACZPAX_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005923418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HIR1113719.1"
                     /translation="MKMISRRSFMAVAGAATAAVALTACGGSKSGSTSTAASTSTAAS
                     SAAAGDAAAAASDPKVTLVYAEVNPLDTIVGQTATHFKEKVEELTGGSV"
BASE COUNT          912 a         1240 c         1248 g          705 t
ORIGIN      
        1 ggagatcaaa cagagcgtat tcgccaacaa cgaccagcgc gccgagcttt tgcgccggga
       61 gctgaaagaa aagaggatct ttctgctcaa cctgatgagc gcaccggggt ccggcaaaac
      121 cacgacgctg cgcagaacca ttgcagccct caaagatgag ctgcgcatcg gtgtgatgga
      181 agcggacatt gactccgatg tggatgcccg cgccatcgcc gggaccgggg caaagtctat
      241 ccagctgcat accggcggta tgtgccacct tgacgccgaa atgacccggc aggggttgga
      301 aggtctgggc accggggaca tcgatttggc gattctcgaa aacgtcggca acctggtctg
      361 ccctgccgag tttgacaccg gcgcagtgaa gaacgccatg attttgtcgg tgccagaggg
      421 ccacgacaaa ccgctgaaat acccgctgat gttccaggta tgcgatatcg tgcttgtgaa
      481 taaaatcgac gtgatgccgt actttgattt tgatctggac aaatgccgag agtacgtcgc
      541 catgcgcaac ccgaaggcga aggtcatccc catctgtgcc aagaccgggg aaggcatcga
      601 cgaatgggca gactggttgc gggagcaggt cagagagtgg cagaggtaaa ggcttccccc
      661 tcgggggaag ctgtccgcga agcggactga tgagggacaa gtttgccatt gccaccattt
      721 accggacagt cgcggcacac ccgcccctca tccggccctg cggggccacc ttccccctcg
      781 ggggaaggct ttggagcata aacggagcaa caacataaaa aggagcacgc catgagcaaa
      841 cccactgcgt tcccgctgga cgagagcaag ctgcccttcc ggatcccgaa ggaccagccg
      901 ttccgggaaa atatcgccaa gctgggccag atgatcactg accgcatccc tgccaaaaag
      961 ggcacgctga cccaggacga ccccgaatac tggggccttg cgtccatcgt gaccgacgag
     1021 atggccgagg ttgcattgaa aatgaaggtc cgcaagccta tgaccctgcc cgagctggtc
     1081 aaggctaccg gcaagcctgc cgacgagctg gaaccgctgc tgcagcagat ggctgtggtc
     1141 ggattactgg aatacaactg ggaaaatccc cgccgggaga agcagtacat cctgccgatg
     1201 ttcgtgcccg gcagtgcgga atttttcaat atgaacaaac agcagattgc cgaccacccc
     1261 gaggtcacgg ccttcttcga gcggatgacc tttttgccgc tggaacacat caccgccatg
     1321 gtgccgccgg gcggcgcggg tatcggtatg cacgtcatcc cggtggaaag ggccatcgag
     1381 accgagaacc agtccgtaga cgtggagcat atctcccact ggctgaaaaa gtatgacggc
     1441 aagtatgccg ccgggccgtg cagctgccgt atgtcccgcg ccgccatggg cgagggctgc
     1501 ggcgacgacc ccgacgactg gtgcatcggc gtcggcgaca tggccgacta ccttgtggag
     1561 acgaacaaag gccactacgt cacctacgac gaggtgatgc agattttgca aaaggccgag
     1621 gacaacggct ttgtacacca gatcacgaac atcgacggcg agaacaagat tttcgccatc
     1681 tgcaactgca acgtcaacgt ctgtaacgct ttgcgcacca gccagctgtt caacacgccc
     1741 aacatgagcc gctcggccta cgttgccaaa gtcgcgccgg aaaactgcgt agcctgcggc
     1801 cgctgtgtgg agtactgccc cgccggtgcg gtcaagctgg gccaaaagct ctgcaccaag
     1861 gaagggcgcg tcgtctaccc gcgccaggaa ctgcccaata ccgtaaagtg gggccccgag
     1921 aagtgggcca tcgactaccg cgacaagaac cgtatcaact gctatgacac cggcacggcc
     1981 ccctgcaaga cggcctgtcc tgcccacatt gccgtgcagg gctacctgaa aatggccgcc
     2041 cagggccgct accgcgacgc gctggcgctg atcaaaaaag agaacccctt ccccgccgtc
     2101 tgcggccgca tctgcaaccg ccggtgcgag gatgcctgca cccgcggcac cgtagaccag
     2161 gctgttgcca tcgacgccgt gaagaagttc atcgcccagc aggacctgca cgccgagcac
     2221 cgctatgtgc ccgacgtcgt ccagccgtcg ctgcaagggc cgtggccgca gaaaatcgcc
     2281 atcatcggcg gcggcccggc gggcctgagc tgcgcctact tcctggcgac ccagggctac
     2341 aagcccaccg tgtttgagaa aaacgaacgt cccggcggta tgctgcggta cggtatcccc
     2401 tccttcaagc tcgagaaaga cgtcattgac gccgagatcg acgtactgcg ggagctgggc
     2461 gtggacatcc gctgcggcgt ggacgtgggc agggatgtga cgctggccca gctgcgcgag
     2521 cagggctaca aggcgttcta cattgccatc ggctgccagg gcggccgcaa ggcgggcgtg
     2581 cccggcgagg atgccgcggg tattgagact gccgtacacc tgctgcgcac cgtgggcggc
     2641 gacgaaagcc ataagatgca gggcaagacc gttgtcatcg gcggcggcaa tgtcgccatc
     2701 gacgcagcgc gggtcgcctt acgctgcggg tccgacgatg tgacaatggt ctgcctggag
     2761 ccgcgggaga agatgcccgc ctcggtggag gaaatcgccg aggccgagga ggaaggcacc
     2821 aaaatcacct gcggctacgg cccgaaggag tttatcgccg aaaacggcca tgtcaccgcc
     2881 gtggtgctga aaaagtgcac cggtctctac aatgccgagg gccgctttgc acccacctac
     2941 gacgagaacg ccaccatcac cctgccttgc gagaacgttg tgctcagcat cggccagtgc
     3001 atcgagtggg gcgacctgct gaacggcgag gccgtagagc tgggccgtgg ccagggtgcc
     3061 gtggccgacg cgctgaccta ccagaccgcg cagcccgacg tgtttgtggg cggcgacgtc
     3121 tacaccggcc cgcgctttgc catcgacgcg attgcggcag gcaaacaggg ggcaatctcc
     3181 atccaccgct atgtgcagcc caacaccagc atgacgatcg gccgcaaccg ccgggatttc
     3241 cacgagctgg acaagaacaa tctgtccctg ggcgagtacg accatgcccc gcgccagacc
     3301 gccgcgctgg acgagagctt cccggaggtg atccgcgcct gcgcccggga gagcgcacag
     3361 acagagcggg agctttgggc acggcgggag gcgaacgcag agcagaatat gcgcaagcgg
     3421 ggcatctgcg tcaccgaact ggacgaggcc gaaaaagccc gcttccgcgc agcggtacag
     3481 cccatgtatg atgcgttctc tgcccagcag gaactgatcg accggataca gaaaagctga
     3541 aaaagacacc ccgagaagtg cccggacgga catttttcgg ggtgttgttt tttgctatga
     3601 atacaggaat gtatccaact ggtcagtagt gttgcacaaa caagacggaa agcagctgtg
     3661 caatgtgtca aaaacaatct tgacattctt tcgaaaaata ggacattttt cgaaagctct
     3721 tgtttcattt cggaaaattt gctaaagtgt gtacagtcaa tgaacgaaag ctccggcacc
     3781 tgtgcgcccg gagaatttca caattattta aggaggacga ttcctatgaa aatgatctct
     3841 cgtcgtagct ttatggccgt tgccggtgca gctactgctg ctgttgctct gaccgcctgc
     3901 ggtggttcca agagcggctc cacttctacc gctgcttcca cctctaccgc agcttcttct
     3961 gctgctgccg gtgatgcagc tgctgctgcc agcgatccca aggtgactct ggtctacgcc
     4021 gaggtcaacc cgctggatac tatcgttggc cagaccgcta cccacttcaa ggagaaggtc
     4081 gaggagctga ccggcggctc cgtcg
//
LOCUS       DBMDSQ010000186         4087 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_5114_length_4087_cov_1.974410, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000186 DBMDSQ010000000
VERSION     DBMDSQ010000186.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 4087)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4087)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4087
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5114_length_4087_cov_1.974410"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..582)
                     /locus_tag="ACZPAX_07180"
     CDS             complement(<1..582)
                     /locus_tag="ACZPAX_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015538323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HIR1113720.1"
                     /translation="MPEGKKNTAPVPAPVRDEDGYSAKTHLHQPVQETATVAATALLA
                     DAPEGALPSEVRPEIVTWEKLCEAIQASGKAYNMEMIQKAYELANNAHNGVCRRSGEP
                     YICHPLAVARLVLDLGMDSESIAAALLHDVVEDTPTTLDDLKAAFGEEVALLVDGVTK
                     LTKIQFSNIEELQAENLRKMLLAMSRDVRVMIIK"
     gene            complement(583..2715)
                     /gene="recJ"
                     /locus_tag="ACZPAX_07185"
     CDS             complement(583..2715)
                     /gene="recJ"
                     /locus_tag="ACZPAX_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015538322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HIR1113721.1"
                     /translation="MTYRAWEQKPLDRTAVRELTAAIAEQAAAQLEEQAMDEAPWSDE
                     KYKAVLAAQQKENALLAGILAARGITDPAEALTLLAGEEELSDPALLTDMDAACQRIW
                     QAIDNGETIAVFGDYDVDGVTATALLYQHLKGMGATVKCMLPSREGDGYGLSKNAIQS
                     MHNKGCTLIVTVDNGISAVEEADFAASLGMDLIITDHHLPPETLPKAVAVVDPRREDD
                     HSPFKGLCGAGVAFKLCAALDGCPPEEMLDYCGDLAAVGTVADVMPLVGENRTLVKAG
                     LRQLQQTDRPGFGALLEEVGLAGKPITAENISYAIAPRINAAGRMDNAVTALQLVLCE
                     DPDRAEELAHKLNEINAHRQETEQQIFKAAEELLEQQPERLDDRIMLLWGRDWHPGVI
                     GIVASRLVERTGRPVIVVTIDEHGEGKGSGRSVQGFNLHACIGSCADLLVRYGGHAMA
                     AGLSVREENLPELRRRLNDWAARECPVLHTPPLTCDVTIHLDRITVESVRHLDQLAPY
                     GAENPTPVFLLQSAVVDGVYPVSEGRHSRLRLRQGNSCLYAVWFGMPAEQLPYALGDV
                     VDAALNLSVYESARGAQLSGRIIDLHPAGLGAELARQAALVQALRRGTPLTEEQKKQI
                     APARTDIIAVYRELQSRRWHAEDLQPLCAKLGEEQTGKTLVAVAALEQVGLITAAEKG
                     GAKFWELVPTAGKKNLADAPILKCLEEL"
     gene            complement(2712..>4087)
                     /locus_tag="ACZPAX_07190"
     CDS             complement(2712..>4087)
                     /locus_tag="ACZPAX_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005940110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HIR1113722.1"
                     /translation="HRQKGEKILMYDRENDNRNNEGRQVQQELEDQLRAFGNTMTDAF
                     AHGFEGRGMDIGDRAWDVGKAAVHAANYGIGEAAKAFRQGRRGYPYGDAKQPFREGAA
                     DADPAGMPGWFRQIFAKPEPTAVESIRISAKKRYSAGCTLLAVGITFAVIFGLGTLGC
                     LIGLGTISPAALGDVVVSATEGGGILMTGTDYVMNTAYNVLGIVSSVLGLATAGFGWM
                     TACGAARMKAGRQMGQFADYADSVDYHKGLPVSMLADLTHQTKKKALKKLRSYIHKGW
                     LNAWLDDETETLYLTAEDYRAAKEAATAAAAQPQPEKTETGDTPLNLDTARRFAAVLE
                     KEQQLMQDAQAREELAAMHKTTTAICDWLEAHPESQPKTRRFAEYYIPTTLKLLHTYN
                     DVQGQQGENAETIRRDIAGILHTLNQAYENLYNNLLSDVAMDISSEIAALQGMLANDG
                     LTGREFE"
BASE COUNT          661 a         1331 c         1252 g          843 t
ORIGIN      
        1 cttgatgatc atcacgcgga cgtcccggct catggccagc agcatcttgc gcagattctc
       61 ggcctgcaat tcctcaatat tggagaactg gatcttggtc agcttggtca cgccgtccac
      121 cagcagggcc acttcctcgc caaaggcggc tttcaggtcg tccagcgtgg tgggggtgtc
      181 ctccactacg tcatgcagca gggcggcggc gatgctctcg gagtccatgc ccagatccag
      241 caccagccgg gcgactgcca gcggatggca gatatagggc tcgccgctgc ggcggcacac
      301 gccgttatgg gcgttgttgg ccagctcgta ggctttttgg atcatctcca tattgtaggc
      361 tttgccgctg gcctgaatgg cctcgcacag cttttcccag gttacgatct ccgggcgcac
      421 ctcagagggc agcgccccct ccggcgcgtc tgccagcagc gccgtagcgg ctacagtggc
      481 ggtctcctgc accggctggt gcagatgagt cttggcagag tagccatcct cgtcccgtac
      541 cggagccggg accggtgcag tgtttttctt tccctcaggc atttatagtt cctccagaca
      601 tttcaggatg ggggcatcgg caaggttctt tttgcctgct gtcggcacca gctcccagaa
      661 ttttgccccg cctttttccg ctgcggtgat cagccccacc tgctctaagg cggcaactgc
      721 caccagcgtt ttgccggtct gctcctcgcc cagctttgcg cacaggggct gcaggtcctc
      781 ggcgtgccag cggcgggact gcaattcccg gtacacggca atgatatccg tgcgggcggg
      841 ggcgatctgt ttcttttgtt cttcagtcag gggggtgccc cggcgcagtg cctgcaccag
      901 tgcagcctgc cgcgccagtt ctgcacccag ccctgccggg tgcaggtcga tgatgcggcc
      961 ggagagctgt gccccccggg cggactcgta gaccgaaagg ttcagtgccg cgtccaccac
     1021 atcgcccaaa gcatagggca gctgctcggc gggcatcccg aaccagacgg cgtacaggca
     1081 gctattgccc tgccgcagcc gcaggcggct gtgccgcccc tccgataccg ggtatacgcc
     1141 gtccaccacc gcgctttgca gcaggaacac cggggtgggg ttctctgccc cgtagggtgc
     1201 cagctgatcc agatggcgca cgctctccac cgtgatacgg tccagatgga tggtcacgtc
     1261 acaggtaagg ggcggggtgt gcagcaccgg gcattcccgg gcagcccagt cgttcaggcg
     1321 gcggcgcagc tcgggcagat tttcttcccg gacggaaagt cccgccgcca tggcgtggcc
     1381 gccgtagcgc accagaaggt cggcacagga gccgatgcag gcgtgcaggt taaagccctg
     1441 cacgctgcgt ccgctgccct tgccctcgcc gtgctcgtca atggtgacca cgatgaccgg
     1501 gcgtccggtg cgctccacca gccgggaagc cacgatgcca atgacgccgg gatgccagtc
     1561 ccgtccccac agcagcatga tgcggtcgtc cagccgctcg ggctgctgtt ccagcaattc
     1621 ctcggcggct ttgaagatct gctgctcggt ctcctgccgg tgggcgttga tctcgttgag
     1681 tttatgcgcc agctcctcgg ctctgtccgg gtcctcgcag agcaccagct gcaaagcggt
     1741 aacggcgtta tccatgcggc ctgccgcgtt gatgcggggc gcgatggcat agctgatatt
     1801 ttcggcggtg ataggcttgc ccgcaaggcc gacttcctct aaaagtgccc caaagccggg
     1861 gcggtcggtc tgctgcagct gccgcagccc ggccttgaca agggtgcggt tctcccctac
     1921 cagcggcatc acgtccgcca cagtgcccac ggcggcaagg tcgccgcagt aatccagcat
     1981 ttcctccggc gggcagccgt ccagcgcggc acaaagcttg aaggctacgc ccgcgccgca
     2041 cagacccttg aaggggctgt ggtcgtcctc tcggcggggg tccaccacgg ccacggcctt
     2101 gggcaaagtc tccggcggca gatggtggtc ggtaatgatc agatccatac ccagcgaagc
     2161 cgcaaagtcg gcttcttcca cggcagagat gccgttatcc accgtcacga tgagagtgca
     2221 gcccttgttg tgcatggact ggatggcgtt tttggacaga ccgtagccat ccccctcgcg
     2281 gctgggcagc atacacttga cggtcgcgcc catgcccttg aggtgctggt acagcagggc
     2341 ggtagcggtc acgccgtcca catcgtaatc gccaaaaacc gcgatggtct cgccgttgtc
     2401 aatggcctgc cagatacgct gacaggcggc gtccatgtcg gtcagcaggg cggggtcgga
     2461 aagctcctcc tcccctgcca gcaaggtcag cgcttcggcg gggtcggtga tgccccgggc
     2521 ggcaaggatg cccgccagca gggcgttttc cttttgctgc gcagccagca cggccttata
     2581 tttttcgtct gaccacggcg cttcgtccat ggcctgctct tccagctgtg ctgctgcctg
     2641 ctccgcaatg gcggcggtca gctcccgcac agcggtgcgg tccagcggtt tttgttccca
     2701 agcgcggtag gtcattcaaa ttccctcccg gtcaggccgt cgttggccag cattccctgc
     2761 aaagcggcga tctcggaaga aatatccatg gctacgtccg aaagcagatt gttgtacagg
     2821 ttctcatacg cctgattcaa ggtatgcagg atgcctgcga tgtcccggcg gatggtctcg
     2881 gcgttttcgc cctgctgccc ctgcacatcg ttataggtgt gcagcagctt gagggtggtg
     2941 ggaatataat attccgcaaa acggcgggtc ttgggctggc tttccgggtg tgcctccagc
     3001 cagtcgcaga tggcggtagt ggtcttgtgc atggcagcca gctcttcccg cgcctgcgcg
     3061 tcctgcatga gctgctgctc tttttccagc acggcagcaa agcggcgggc ggtatcgaga
     3121 ttgagcggag tatcgccggt ttccgtcttt tccggctgcg gctgcgcggc ggcagcggta
     3181 gctgcctcct tggcggcgcg gtagtcctct gccgtcaggt agagcgtttc ggtctcatcg
     3241 tccagccatg cgttcagcca gcccttatga atgtagctgc gcagcttttt cagcgctttt
     3301 ttcttggtct ggtgggtcag gtcggccagc atcgaaacag gcaggccctt atggtagtcc
     3361 acgctgtcgg cgtagtcggc aaactggccc atctgtctgc ccgccttcat ccgggctgca
     3421 ccacaggccg tcatccagcc aaagcccgcc gtggcaaggc ccagcacgct gctgacgatg
     3481 cccagcacgt tgtaggcggt gttcataacg tagtccgtgc ccgtcatcag gatgccgccg
     3541 ccctcagtag ccgacaccac aacgtcaccc aaagcagccg gcgaaatagt gcccagcccg
     3601 atgaggcagc ccagcgtccc cagcccgaag atcaccgcaa aggtgatgcc cacagccagc
     3661 aaagtgcaac ctgcgctgta ccgctttttg gcgctgatac ggatactttc caccgcggtc
     3721 ggctccggct ttgcaaaaat ctgccggaac cagccgggca tcccggcggg gtcggcatcc
     3781 gcagcacctt cgcggaaagg ctgctttgca tccccgtagg gataacctct gcgcccctgc
     3841 cggaaggcct ttgcggcctc tccgatgccg tagttggctg catggacagc ggccttgccc
     3901 acatcccacg cccggtcgcc gatgtccatg cccctgccct caaagccgtg ggcaaaggca
     3961 tcggtcatgg tgttgccaaa ggcgcgcagc tggtcttcca gctcctgctg cacctgtcgg
     4021 ccttcgttat ttcggttatc gttttcacga tcgtacatca aaattttctc acccttctgc
     4081 cggtgca
//
LOCUS       DBMDSQ010000187         4075 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_5134_length_4075_cov_1.433065, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000187 DBMDSQ010000000
VERSION     DBMDSQ010000187.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 4075)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4075)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4075
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5134_length_4075_cov_1.433065"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..268)
                     /locus_tag="ACZPAX_07195"
     CDS             complement(<1..268)
                     /locus_tag="ACZPAX_07195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113723.1"
                     /translation="MSLLKKLAGLAVGAAAVGAAVYVLQNREHHDDEYEHIWGSEDEV
                     EPETDSAEPADDASAVAEPAPAAAPEEPAVPAEEEKPEEPAEVEE"
     gene            461..820
                     /locus_tag="ACZPAX_07200"
     CDS             461..820
                     /locus_tag="ACZPAX_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016148401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HIR1113724.1"
                     /translation="MKYIFQFARITAFCLAGEVLAVVLPLPIPASVYGLLLMVAALKT
                     GVLKLDQVRETGLFLTGIFPLLFVPAAAGVVELGSQLMDLLLPAVLAIVPITALVMAV
                     TGMVAQKCAGRKEHKNG"
     gene            813..1508
                     /locus_tag="ACZPAX_07205"
     CDS             813..1508
                     /locus_tag="ACZPAX_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HIR1113725.1"
                     /translation="MAEFLQQCGSWGVLATLAAYAAGVWVNRKTGKALFNPLLMGSIF
                     VIVFLSCFGVPYADYKSSAQPISWLLMPATVSLAIPLYEKWELLEKNLAAIFAAIAAG
                     VLTSLGSVLAMAWVLRIERTHAVSFLPKSVTTAIGMDVAETLGGAAALAGAVIILTGI
                     VGSLMGETVCKVCHITDPLAKGLALGTSAHAIGTSKALQMGEVEGAMSGLAIAVAGIM
                     TAILAPVAANFLP"
     gene            complement(1804..2181)
                     /locus_tag="ACZPAX_07210"
     CDS             complement(1804..2181)
                     /locus_tag="ACZPAX_07210"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA];
                     GO_process: GO:0016075 - rRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HIR1113726.1"
                     /translation="MEVHRGEVFYADLSPVVGSEQGGIRPVLIIQNEIGNRHSPTVIA
                     AAITSRQDKNRLPTHISVRADRCGLAKDSIILTEQIRTLDKRRLRERAGIIPPDDMRR
                     VDEALGVSMGLESADVHTDTGYF"
     gene            complement(2235..3323)
                     /locus_tag="ACZPAX_07215"
     CDS             complement(2235..3323)
                     /locus_tag="ACZPAX_07215"
                     /inference="COORDINATES: protein motif:HMM:NF013884.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HIR1113727.1"
                     /translation="MPGKTQRSSNLELLRILCMLLIIGDHLTGQGGIADYTTPPSSFA
                     FCLIGCGSRIACSVFILIGGWFLCEQPYKTRRPLSLWLSLWLYTVPVTLLCRLAGLDV
                     SFGALRWAAFPASTRQLWFISDYLLLLLCVPLLNRVLRGLPRTAHRGLLAVLAVPMIL
                     YPTLFGENGIVSDTAWMFLYEYLLAAYLRRYPENRLARLLQNRAAALALGLGLPLVNT
                     AIRAMLETRGLTDGKAFQYVAYYRTALGALPNLLAALALFYLFKNLDLGCVRWVNALS
                     GTTLGVYILHQVPAFRNFLWNGLLQAQAHHGSVVYTLFAIFAVLFGCAAVDAVRTRLV
                     MRPLENTKLFTALCEKGDALAAKITADD"
     gene            complement(3437..3817)
                     /gene="acpS"
                     /locus_tag="ACZPAX_07220"
     CDS             complement(3437..3817)
                     /gene="acpS"
                     /locus_tag="ACZPAX_07220"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HIR1113728.1"
                     /translation="MILGVGIDLCDAGRMEKSITRQSFLDHVFAPEEQALLAALGDKH
                     RAETAAANFAAKEAFLKAAGTGLGGFAMPELAVLRRESGAPYFALTGAAAAWAAEQNL
                     TVHVSLSHERGLASAVVILETGTP"
     gene            complement(3814..>4075)
                     /locus_tag="ACZPAX_07225"
     CDS             complement(3814..>4075)
                     /locus_tag="ACZPAX_07225"
                     /inference="COORDINATES: protein motif:HMM:NF025112.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113729.1"
                     /translation="HGGGHRQVGLAGARRADVEAFEHRMDELAKTGCYTMRQLTVNGA
                     DLMDAGIPTGPDVGATLQMLLTAVMEGKLPNEREALLKAVIS"
BASE COUNT          813 a         1228 c         1265 g          769 t
ORIGIN      
        1 cctcttcaac ttccgcgggt tcctcgggct tttcctcctc ggcagggacg gcaggttctt
       61 caggtgcggc ggcgggggcg ggttcagcca cagcggacgc atcgtcagcg ggctcggcgg
      121 aatcggtctc aggctcgacc tcgtcctccg aaccccaaat atgctcgtac tcgtcgtcgt
      181 gatgctcacg gttctgcaga acataaacgg cagcgcccac agcagcggcg cccacggcca
      241 aaccggccag ttttttcagt aaagacatgg tcaaaacctc ttttcgagta aaaatcttgt
      301 gtttgcggtt agtataccac aatcgtttcc aaaatacaag tgccgaatcc tacaacgcag
      361 gtgggaaaaa gtggacgctt ttcatacaat gtacaacttg tcgaaaatgg agcggtgtgg
      421 tatgatgtac aggaattttg caaaaagagg gtctaataag gtgaagtata ttttccagtt
      481 tgcccgcatc acggcgttct gcctggcggg cgaggttctg gcggtggtgc tgccgctgcc
      541 catcccggcc agcgtgtacg gcctgctgct gatggtcgcg gcgctgaaaa caggcgtgct
      601 caagctcgac caggtgcggg agacagggct gtttttgacc ggcatcttcc cgctgctgtt
      661 cgtcccggcg gcggccggcg ttgtggagct gggcagccag cttatggatt tgctgctgcc
      721 cgccgtgctg gctatcgtgc ccatcacggc gctggtcatg gccgtgaccg gcatggtcgc
      781 gcagaaatgc gcaggcagaa aggagcacaa aaatggctga atttttgcag caatgcggca
      841 gctggggcgt gctggcgacg ctggcggcct acgcggcggg cgtctgggtc aaccgcaaga
      901 ccggcaaggc actgttcaat cccctgctga tgggcagcat cttcgtcatc gtgtttttaa
      961 gctgcttcgg cgtgccctac gccgactata aatccagcgc ccagcccatc agctggctgc
     1021 tgatgcccgc cacggtcagc ctggcgatcc ccctgtatga aaaatgggag ctgctggaaa
     1081 agaacctggc cgcgatcttt gcggccattg cggcgggtgt gctgacgagc ttgggcagcg
     1141 tgctggcgat ggcgtgggtg ctgagaatcg agcgcaccca tgcggtgagc tttctgccca
     1201 agtcggtcac gacggccatc ggtatggatg tggccgagac gctgggcggt gcggcggcgc
     1261 tggcgggcgc ggtcatcatt ctgaccggca ttgttggcag cctgatgggt gagacggtct
     1321 gcaaggtctg ccatattaca gacccgctgg ccaaggggct ggcactgggc acctcggccc
     1381 atgccatcgg cacgagcaaa gccttacaga tgggcgaggt agaaggcgcc atgagcgggc
     1441 ttgcgattgc ggttgcgggt atcatgaccg cgatcctggc cccggtggcg gcgaactttt
     1501 tgccgtaaag gggcgataag ggaaagagac ggggcgtcga ggacgccgcc cccgaggcgc
     1561 gggtcccgct tgacggcgca caacgagcgc cgccagtttg cttcgcaaac ccgctcttgc
     1621 ggttgtttgc cccacagggg caaacattgt tcgccacccc agtttcgctt cgctcaactg
     1681 tggcccctga ctcacaaccc cgcaccacgt cgcaacagcg gcagttttct ttgccagctt
     1741 tcttttgcgg ccaaaagaaa gctgcttccc gggagcctgc cgtttatcct ttagccgaca
     1801 ctctcaaaaa tacccggtat ccgtgtgcac atccgcgctt tccagtccca tggatacccc
     1861 taacgcttca tccacgcggc gcatgtcgtc gggcgggatg atacctgccc gctcccgcag
     1921 gcggcgcttg tcgagggtgc gaatctgttc ggtcaaaatg atactgtcct tcgccaggcc
     1981 gcagcggtcg gcccgcacgc tgatatgtgt gggcagccgg tttttgtcct ggcggctcgt
     2041 gatggctgcc gcaatgacgg tgggactgtg ccggttgccg atctcattct ggatgataag
     2101 taccggccgt atgcccccct gctcggaccc gacgaccggg cttaagtcgg cgtagaaaac
     2161 ctctccgcgg tggacttcca taagaaaaac ctccctttgc ttgttactca aagtataagc
     2221 agagggaggt ctttttaatc atccgccgtg atttttgcag cgagggcatc gcctttttca
     2281 cacagcgctg taaacagctt ggtgttttcc aagggccgca tcaccagccg ggtgcgcacg
     2341 gcgtccactg ccgcacaccc gaagagcacg gcaaagatcg cgaacagcgt gtacaccacc
     2401 gagccgtggt gcgcctgggc ctgcaaaagg ccgttccaca aaaagttgcg gaacgcgggc
     2461 acctgatgca gaatgtaaac gcccagcgtc gtgccggaca gggcgttgac ccaccgcaca
     2521 cagccgagat ccaaattttt aaacaggtag aacagcgcca gcgcggccag caggttgggc
     2581 agtgcaccca gtgccgtacg gtagtaggcc acgtactgga acgccttgcc gtcagtcaga
     2641 ccgcgcgttt ccagcatagc gcggatggcc gtgttgacca gcggcagccc cagcccaagg
     2701 gccagcgccg ccgcgcggtt ttgcaaaagg cgggccaggc ggttttctgg atagcggcgc
     2761 aggtaggcgg ccagcaggta ctcatacaaa aacatccagg cagtgtcgct gacgatgccg
     2821 ttctcgccga aaagcgtcgg gtagaggatc atcggaacgg ccagcacggc cagcagcccc
     2881 cggtgcgcgg tgcgcggcag gccgcgcagt acgcggttca gcagcggcac gcagagcaac
     2941 agcagcagat agtccgagat aaaccacagc tgccgggtcg aggccgggaa cgccgcccac
     3001 cgcagtgcac cgaaggaaac atccagcccg gcgagcctgc acagcagcgt gacaggcacc
     3061 gtgtagagcc acagcgacag ccacagcgag agcgggcggc gtgttttgta cggctgttcg
     3121 cacaaaaacc acccaccgat gaggatgaac acactgcacg caatgcgcga cccgcagccg
     3181 atgaggcaga atgcaaagga ggacggcggc gtggtatagt ccgcaatgcc gccctgcccg
     3241 gtcagatggt cgccgatgat gagcagcata cacaggatgc gcagcaattc taaattgctg
     3301 ctgcgctggg tcttgccggg catgggaacc tccttgaaaa atgacagcaa aattgtaagg
     3361 gccggacata ccaggcccgc agcctataaa taggagcgca tttgcgggaa agctgagggc
     3421 tgggcaagcc cggcccctac ggggtgcctg tttccagaat caccactgca ctcgcaagtc
     3481 cacgttcatg gctcaagctg acatgcacag tcaaattctg ctccgcagcc caggcggcgg
     3541 cagcgccggt cagggcaaag tacggtgcgc cgctttcccg gcgcagcacg gcaagctcgg
     3601 gcatggcaaa gccgcccagg ccggtgccag cggccttcaa aaaggcctcc ttcgccgcaa
     3661 agttcgcagc ggcagtctcg gcccggtgct tatcccccaa agcggccagc agggcctgtt
     3721 cttcgggggc aaatacatgg tcaagaaagc tctgccgagt gatgcttttt tccatgcggc
     3781 ctgcatcaca caggtcaatg ccgacgccca aaatcatgaa atcaccgctt tcagcagtgc
     3841 ttcccgctcg ttgggcagtt ttccttccat gacggcagtc agcagcattt gcagggtcgc
     3901 gccgacgtcc ggcccggtgg ggatgccggc atccattaaa tccgcgccgt tcacggtcag
     3961 ctggcgcatg gtgtagcagc cggtcttggc aagttcgtcc atgcggtgct caaaggcctc
     4021 gacatcggcc cggcgggcac cggcaagacc tacctggcgg tggccgccgc cgtgg
//
LOCUS       DBMDSQ010000188         4055 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_5170_length_4055_cov_1.643399, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000188 DBMDSQ010000000
VERSION     DBMDSQ010000188.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 4055)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4055)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4055
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5170_length_4055_cov_1.643399"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1231)
                     /locus_tag="ACZPAX_07230"
     CDS             complement(<1..1231)
                     /locus_tag="ACZPAX_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002608396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR1113730.1"
                     /translation="MRGIYTPVTDIRRKVFTEVARMAYEVNELSDYEQLMRELPFKII
                     PGEEKSLRSSIFLERAIISERVRLAMGMSLRPLDESVPASEGLEHSVIADKYYEPPLI
                     NVIKFACNACPEKVIKVTAMCQGCLAHPCQEVCPKHAISFRNGKSHIDQSLCVKCGRC
                     VNSCPYSAIVKTERPCAAACGMGAIHSDEHGRAEIDYDKCVSCGMCLVNCPFGAIVDK
                     GQIFQLIQSIKRGDEVVAIVAPAFINQFPGMTPAKLKEAMRQLGFADVAEVAIGADLC
                     TIDEAKDFMEEVPAKIPFMGTSCCPAWSVMAKKLFPQQAECISMAMTPMVLTARLIKK
                     EKPNARICFVGPCAAKKLEASRRSVRSEVDFVLTFEELMGLFEAKAVNFADLSDNQED
                     AFNSASADARGFAASGGV"
     gene            complement(1396..2694)
                     /gene="mtaB"
                     /locus_tag="ACZPAX_07235"
     CDS             complement(1396..2694)
                     /gene="mtaB"
                     /locus_tag="ACZPAX_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015563702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035598 - tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HIR1113731.1"
                     /translation="MRVTFYTLGCKVNQNETGALAQLFEENGYTVVSNEEGADVYIVN
                     SCTVTNFGDQKSRKWLRRAKRENPGAVTVLTGCYPQAFPEEAAEIAEADVVTGSGNRH
                     AILADVQKVLNGEEERVVDIRPHEKGERFEELPMDKFAEHTRAFVKVEDGCNRRCAYC
                     VIPRARGPVRSRDEASVLKELRRLSDAGYKEVVLTAISLPSYGIDSGTSLVELIEKAA
                     EIPGIERIRLGSLDPDMLHDEDILRMAGVKKLCPQFHLSLQSGCDKTLRAMRRPYTTA
                     QYADIAAKLREAFGAEKVSFTTDVIVGFPGETEEDFEASMAFVTGMQFLKVHVFPYSR
                     REGTAAYDFPGQVPEHEKESRSRRMTAAVEEVRAAEVAAMQGRTASVLLETPLSNTLF
                     TGYTKQYLPVLVSAPGHKTGDIVDVTLGAWDGKRCRAESI"
     gene            2822..3997
                     /locus_tag="ACZPAX_07240"
     CDS             2822..3997
                     /locus_tag="ACZPAX_07240"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HIR1113732.1"
                     /translation="MKKRVITLLLCLVLAVLSILRGFAASAEGITPATIADFDVPCAA
                     AILIDEDSGTVLYEKNADDRRPIASITKVMTLLLTFEALEAGKIHLDDFVPVSEHAYH
                     MGGSQIWLEPGEQMTLDDMLKAICISSANDAAVAVAEYVGGSETAFAQQMNTRAAELG
                     MTSTHFSNACGLDEEGHLSSARDVALMSREILLHHAEVRNYCSIWMDTLRGGATQLVN
                     TNKLLKSYSGITGLKTGTTGKAGVCISASAERDGLRLIAVVLGSASGKERFQAASALL
                     DYGFAHYESAAAELPADAPQTLSVTHGTAETVTLTYTAPNRCLMPKGEGTALEAAIDL
                     PEKLEAPVTAGQTVGTVTIRNNGAEVQAYPITAAQDVDALSFGYCFRKLAQSLVALD"
BASE COUNT          799 a         1212 c         1205 g          839 t
ORIGIN      
        1 caacaccgcc ggaagctgca aagccacggg catcagcact ggcgctgttg aaggcatcct
       61 cctggttgtc ggacaggtcc gcaaagttga cagccttcgc ttcgaacagg cccatcagtt
      121 cctcaaaggt cagcacgaaa tcgacctcac tgcggacgga gcggcggctg gcttccagct
      181 tcttggcagc gcaggggccg acgaagcaga tgcgggcgtt gggcttttcc ttcttgatga
      241 ggcgggcggt caggaccatc ggggtcatgg ccatgctgat gcactcggcc tgctggggga
      301 acagcttctt cgccataacg ctccatgccg ggcagcagga ggtgcccatg aacggaatct
      361 tggcggggac ttcctccatg aagtcctttg cctcgtcgat ggtgcacagg tcagcaccga
      421 tagcgacctc ggccacatcg gcgaagccca gctgacgcat ggcctccttc agcttggcgg
      481 gggtcatacc ggggaactga ttgatgaagg cgggggcgac gatggcaacg acctcgtcac
      541 cgcgcttgat ggactggatc agctggaaga tctgaccctt gtcgacgatg gcaccgaacg
      601 ggcagttgac aaggcacata ccgcaggaaa cgcacttatc gtaatcgatc tcggcacggc
      661 cgtgctcgtc agagtggatc gcacccatgc cgcaggcggc ggcgcaggga cgctcggtct
      721 tgacgatggc gctgtagggg cagctgttga cgcagcggcc gcacttgacg cacaggctct
      781 ggtcaatgtg gctcttgccg ttgcggaagc tgatggcgtg cttggggcag acctcctggc
      841 agggatgcgc cagacagccc tggcacatgg ccgtgacctt gatgaccttc tccgggcagg
      901 cgttgcaggc aaacttgatg acgttgatca gcggcggctc gtagtacttg tctgcgatga
      961 cgctgtgctc cagaccctcg ctggcaggga cgctctcgtc cagcgggcgc agggacatac
     1021 ccatagccag gcgaacgcgc tcagagatga tggctctctc caggaagata ctgctacgca
     1081 gggatttctc ctcgccgggg atgatcttga agggcagctc acgcatcagc tgctcgtaat
     1141 cggaaagctc gttgacctca taggccatgc gggcaacctc agtgaacacc ttgcgacgga
     1201 tatccgttac gggggtgtaa atgcctctca ttctaattgc tccttttttt gctccgtttg
     1261 ctcttaaatg gacttatcgg cgggcatacc cgctcctgtt aaaattttac caaaaatcca
     1321 tgctttttgc aagaggacag gcaacaaaaa acaggaaaaa taagcatatt ttttgcttcg
     1381 cagtaaatgt agctctcaga tactctccgc ccggcagcgt ttgccgtccc acgctcccag
     1441 cgtcacatcc acaatatccc cggttttgtg cccgggggcg ctgaccagca ccggcaggta
     1501 ctgcttggtg tagccggtga acagcgtgtt ggacagcggc gtttccagca gtacactggc
     1561 cgtgcggccc tgcatggcgg ccacctcggc ggcgcgtact tcctccacag cggcggtcat
     1621 acggcggctg cgggattcct tctcgtgctc tggcacctgg ccggggaagt catacgcggc
     1681 tgttccctcg cggcgggagt aggggaacac gtgtaccttc aaaaactgca tcccggtcac
     1741 gaaagccatg ctggcttcaa agtcttcctc ggtctcaccg ggaaaaccga caatgacgtc
     1801 ggtcgtaaag ctgaccttct ccgcaccgaa ggcttcccgc agtttggcgg caatgtcggc
     1861 atactgggcc gtggtgtagg ggcggcgcat ggcgcgcagc gtcttatcgc agccgctttg
     1921 caggctcaaa tggaactgcg ggcagagttt tttcacgccc gccatccgga gaatatcctc
     1981 gtcgtgcagc atatccgggt ccaggctgcc aaggcggata cgctcaatgc caggtatctc
     2041 ggcggctttc tcaatcagtt ccaccaggct tgtgccgctg tcgatgccgt agctgggcag
     2101 gctgatggcc gtcagcacga cctctttata gcctgcgtcg gacagacggc gcagttcttt
     2161 cagcacgctg gcctcgtctc ggctgcgcac cgggccgcgg gcgcggggaa tgacgcagta
     2221 ggcacagcgg cggttgcagc catcctcgac cttaacaaag gcgcgagtgt gctcggcgaa
     2281 cttatccatg ggcagttctt caaaccgttc tcccttctcg tggggacgga tatctaccac
     2341 gcgctcctcc tcgccgttca gcaccttctg cacatcggcc aaaatcgcgt gccggttgcc
     2401 ggagccggtc acaacgtcag cctcggcgat ctcggcggcc tcctcgggga aagcctgggg
     2461 atagcagccg gtcaacaccg tcaccgcgcc ggggttctcg cgcttggcgc ggcgcagcca
     2521 cttacggctt ttctggtcac cgaaattcgt gacggtgcag ctgttgacga tatagacatc
     2581 ggccccttcc tcgttggaga cgacggtgta gccgttttcc tcaaacagct gtgccagtgc
     2641 gccggtttcg ttctgattta ccttgcagcc caaggtgtaa aaagttaccc gcatggtttt
     2701 gtgtccttta catatagaat agctttccta ttataataga aaaaccacga aagtgcaata
     2761 ctaattccag gcgccgccgc atagctttag acaaagcgat ttccgccgga ggtgtgctgc
     2821 catgaaaaaa cgtgtcataa ctctcttgct ctgccttgta ttggcggtgc tgtccatcct
     2881 gcgcgggttt gctgcgtccg ctgaaggcat cacccctgcc acaatcgccg actttgacgt
     2941 gccctgcgcg gcggctatcc tcattgatga ggactccggc acagtgctgt acgagaaaaa
     3001 cgccgacgac cgccgcccca ttgcgtccat caccaaagtt atgacgctgc tgctgacctt
     3061 tgaggcactg gaagcgggga agatacacct ggacgatttt gtcccggtca gcgagcatgc
     3121 ctaccacatg ggcggcagcc agatatggct ggaacccggc gagcagatga cgcttgacga
     3181 tatgctcaag gctatctgta tttccagcgc caacgatgcc gctgtggcgg tggccgagta
     3241 tgtcggcggc agtgaaacgg cgtttgccca gcagatgaac acccgcgccg ccgagctggg
     3301 catgacgagc acccactttt ccaatgcctg cggcctggac gaggaaggcc acctctcgtc
     3361 agcccgggat gtggccctca tgagccgcga aattctcctg catcatgccg aggtgcggaa
     3421 ctactgctcc atctggatgg atactctgcg cggcggcgca acgcagttgg tcaacaccaa
     3481 caaactgctg aaaagctact ccggcatcac ggggctgaag accggcacca ccggcaaggc
     3541 gggcgtctgc atctccgcca gtgccgagcg cgacggcctg cggttgattg ccgttgtgct
     3601 ggggtctgcc tcgggcaagg agcgtttcca ggctgccagc gcgctgttgg actacggctt
     3661 tgcccactat gagagcgctg ccgctgagct gcccgccgat gcgccgcaga cgctgtccgt
     3721 cacccacggc actgcggaaa ccgtgacact gacctatacg gccccgaatc gctgcctgat
     3781 gcctaagggc gagggcacgg cgttggaagc cgctatcgac ctgcccgaaa agctggaagc
     3841 cccggtgacg gcagggcaga ctgtcggtac tgtgacgata cgcaataacg gcgcggaagt
     3901 gcaggcatac cccatcacgg cagcacagga cgttgatgcg ttaagtttcg gctattgctt
     3961 ccgcaaattg gcacaaagcc tggtggcttt ggattgattt tgtgcaaatt tcatgaaaaa
     4021 gtcgaaaaac ccccccggct tttgcttgac aatac
//
LOCUS       DBMDSQ010000189         4031 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_5203_length_4031_cov_1.838677, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000189 DBMDSQ010000000
VERSION     DBMDSQ010000189.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 4031)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4031)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4031
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5203_length_4031_cov_1.838677"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..208)
                     /locus_tag="ACZPAX_07245"
     CDS             complement(<1..208)
                     /locus_tag="ACZPAX_07245"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005921018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent flavin oxidoreductase"
                     /protein_id="HIR1113733.1"
                     /translation="MKLLNEILGTKYPIIQGGMANIATGEFAAACSNAGALGLIGSGG
                     MDADSLRENIRRCRALTDKRFGVNI"
     gene            complement(205..1158)
                     /locus_tag="ACZPAX_07250"
     CDS             complement(205..1158)
                     /locus_tag="ACZPAX_07250"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005921020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase 3"
                     /protein_id="HIR1113734.1"
                     /translation="MKGLQLLATGGALPNRVVTNEDLRRQVDTSDEWITTRTGICQRY
                     YCTENEDAATLAITAARQALTHSGLTANDIACCVVATLSAPTATPSIACRVQAALGLP
                     EDRPAFDVNAACSGFIYAAAAAHGLLNTLGGRYALVIGCEALSRMVDPTDRTTCVLFG
                     DGAGAAIFELAENVPFAVTLGARGSEAIRAGGPAARDTAPITMDGKAVFRFAVEAMPR
                     CLQAVLDRSQKTLADLDWVVCHQANSRIIDHCVKSLHAEPSKFYKNISRHGNTSAASI
                     PIALHEMAESDLLRPGQTLACIGFGGGLTWGGMLLTYEGRK"
     gene            complement(1155..2114)
                     /locus_tag="ACZPAX_07255"
     CDS             complement(1155..2114)
                     /locus_tag="ACZPAX_07255"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005935299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HIR1113735.1"
                     /translation="MIKEVLQQLEPLDAQIDALCSRADDAEMANTTAHAAELAELSAE
                     ENALLQRCTSLTAEDRVFLARRPDRPHITEIIDNLFTDFFEQCGDRQCKEDAAILCGI
                     ARFHGMPVTVLGHRKGTTLEENLRCNFGMPGPEGYRKALRVMRQAEKFHRPIITFIDT
                     PGAYPGKDAEERGQGEAIARNLMEMSGLTVPVLAIVTGEGSSGGALGLGVANHILMLE
                     NAVYSVLSPEGFASILWKDASRSGEACTMMKLTAQDLYADGIVEEIIPEPLGGAQRGR
                     QLLFSRLDTAIAVHLAALCKMSGKALADQRYKKYRRIGEMRGA"
     gene            complement(2111..2995)
                     /gene="accD"
                     /locus_tag="ACZPAX_07260"
     CDS             complement(2111..2995)
                     /gene="accD"
                     /locus_tag="ACZPAX_07260"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015565117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HIR1113736.1"
                     /translation="MSAIEKLFPTSPQERSFQMKARRDAGTAAPNWVPPQFVACKKCG
                     RRATRQVWTKSLYVCPNCGVHLPIGAYYRFSTVLDHGTFKELNPDIAPNDVLHFPDYP
                     EKLAAASVKTGLKEAAVTATGKIGGVQAVVAVLDSRFFMGSMSTAVGEKITRAIEYAA
                     DKHLPLIIFSASGGARMQEGIFSLMQMAKTSAALERFNRSGGLYISVLTHPTTGGVTA
                     SFASLGDITLAEPGALIGFAGPRVIEQTIGEKLPDGFQRSEFQLEHGFVDQVVPRTEL
                     KEVLTRLLQLHTQGRKRR"
     gene            complement(3009..3239)
                     /gene="acpP"
                     /locus_tag="ACZPAX_07265"
     CDS             complement(3009..3239)
                     /gene="acpP"
                     /locus_tag="ACZPAX_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HIR1113737.1"
                     /translation="MNFEELKEVFVDTLNCDADKVTMDASLTDDLQADSLDAVELNMA
                     FEDKFGVTIADEDLGTLKTVGDIFNYLAARAA"
     gene            3597..>4031
                     /locus_tag="ACZPAX_07270"
     CDS             3597..>4031
                     /locus_tag="ACZPAX_07270"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005930504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HIR1113738.1"
                     /translation="MKRSGILLQYNAPVTLTFFFLSLASLILGYLTNNWTTSHLFSVY
                     RSSMTDPLFYVRLFGHVLGHGGWNHFINNMLLFLVIAPPLEERYGSRTLLSGILMTAL
                     VSGILQCVLFPTSALLGASGIVFMLIMLSSLAGSKNGGIPITF"
BASE COUNT          828 a         1180 c         1064 g          959 t
ORIGIN      
        1 tgatgttcac gccaaagcgc ttatcggtca gggcgcggca gcggcggatg ttttcccgca
       61 gggaatccgc gtccatgccg ccggagccga tcagccccag cgccccggca ttggaacagg
      121 cagcggcaaa ctcgcctgtg gcgatattgg ccatgccgcc ctgtataatg ggatattttg
      181 tacctaaaat ttcattgagc agcttcattt tctaccctca tacgtcagca gcatcccgcc
      241 ccatgtcaga ccgccgccaa agccgatgca ggccagcgtc tggccggggc gcagcaaatc
      301 actttccgcc atttcatgca gggcaatcgg gatgctggca gcactggtgt tgccgtgacg
      361 gcttatgttt ttgtaaaatt ttgacggttc tgcgtgtaag cttttgacac aatggtcaat
      421 tatgcggctg ttggcctggt ggcagactac ccagtccagg tcagccaatg tcttttggct
      481 gcggtccagc acggcttgca gacagcgcgg catagcctcc acggcaaagc ggaaaacagc
      541 cttgccgtcc atcgtgatcg gtgcggtatc gcgggccgca gggccgcccg cgcggatcgc
      601 ttcactgccc cgtgcgccta aagttacggc gaacggtaca ttttcggcca gttcaaaaat
      661 agccgcaccc gcgccatcgc caaataatac acaggtcgtg cggtcggtcg gatccaccat
      721 gcgggagagt gcctcacagc caatgaccag cgcataccgc ccgcccagcg tgttcagaag
      781 cccgtgggcc gcggcggcgg cataaataaa gcccgagcag gccgcattca catcaaaggc
      841 aggccggtct tccggcagac ccaaggcagc ctgtacgcgg caggcaatgc ttggggtagc
      901 cgttggggcc gagagcgttg ccacaacaca gcaggcaatg tcgtttgccg tcagaccgct
      961 gtgggtcagg gcctgacggg cggcggtgat ggccagcgtg gcggcatcct cgttttcggt
     1021 gcagtaatag cgctggcaga tgccggtgcg ggtcgtgatc cattcatcgc tggtgtccac
     1081 ttgccggcgc agatcctcgt tcgtcaccac acggttgggc aatgccccac cggtagccag
     1141 cagctgtaaa cctttcatgc gcccctcatt tctccgatgc ggcggtattt tttgtatcgc
     1201 tggtcggcca aggccttgcc gctcatcttg cataaggcag caaggtgtac cgcaatggcg
     1261 gtgtccaacc ggctgaaaag cagctgccgc ccacgctgtg cgccgcccag cggttcgggg
     1321 atgatctctt ccacgatgcc atctgcgtac aggtcctgtg ccgtcagctt catcatggtg
     1381 caggcctcac cgctgcggga agcatctttc cacaaaatgc tggcaaaccc ctcgggactg
     1441 agcactgagt acacggcatt ttccagcatc agaatgtggt tggcaacgcc cagccccagt
     1501 gcgccgccgc tggatccctc gcctgtgaca atggccaaca ccgggacggt caggccgctc
     1561 atctccatca ggttgcgggc aatggcttcc ccctgaccgc gttcttcagc atccttgcca
     1621 gggtaagcac cgggagtgtc aataaaagtg atgatgggac ggtgaaattt ttctgcctgc
     1681 ctcatcacgc gcagggcctt gcggtaaccc tccggtcccg gcatgccaaa gttgcagcgc
     1741 aggttttctt ccagcgtggt ccctttgcgg tgccccaaaa cggtaacggg catcccgtgg
     1801 aaccgtgcga ttccgcataa aatggccgca tcctctttgc attgtcggtc gccgcactgc
     1861 tcaaaaaagt cagtaaacaa gttatcgatt atttcggtaa tgtgcgggcg gtcagggcgg
     1921 cgggccagga aaacacgatc ctccgccgtc agcgaggtgc agcgctgtaa aagcgcattt
     1981 tcctctgcgg aaagctcggc cagttcggcc gcatgggcgg tcgtgttcgc catttcggcg
     2041 tcatcggcac ggctgcacag cgcgtcgatc tgcgcgtcca gcggttccag ttgctgcaaa
     2101 acttccttga tcatctgcgc ttcctcccct gtgtgtggag ctgcaagagc cgtgttaaga
     2161 cctcttttaa ctcggtgcgc ggcacgacct gatccacaaa gccgtgttcc agctggaact
     2221 cgctgcgttg gaacccgtcg ggcagttttt caccgatggt ctgttcaatg acgcgcggcc
     2281 ctgcaaagcc gatgagcgcc cccggttcgg cgagcgtaat atcacccaga ctggcaaagc
     2341 ttgccgtaac tccgcctgtg gtggggtgag tcagcacact gatgtacaac ccgccgctgc
     2401 ggttgaaccg ttccagcgca gcgctggttt tggccatctg catcaggctg aaaataccct
     2461 cctgcatccg tgcgccgccg ctggcagaaa aaatgatgag cggcaggtgc ttgtcggcag
     2521 cgtactcgat cgcacgtgtg attttctcac caacagcggt gctcatgctg cccataaaaa
     2581 agcggctgtc caacacagcg accaccgcct gcacgccgcc gatcttgccg gttgctgtaa
     2641 cggcggcttc tttcaggccg gtcttgaccg aagcagcggc cagcttttcc ggataatcag
     2701 gaaaatgcag cacatcgttg ggcgcaatgt ccgggttcag ttctttaaag gttccgtgat
     2761 ccaaaaccgt actaaaccga taataggccc cgatgggcag atgcaccccg cagttggggc
     2821 aaacatacag gcttttggtc caaacctgac gggtggcgcg gcggccgcat tttttgcagg
     2881 cgacaaattg cggcggcacc cagttgggcg ctgccgtacc ggcatcgcgg cgggctttca
     2941 tctgaaagct gcgctcctgc gggctggtcg ggaacagctt ttcaatggca ctcattccgc
     3001 aatacccttt aggctgcccg tgcagccaga tagttgaaga tatcgcccac ggttttaagc
     3061 gtccccagat cctcgtcagc gatcgtgacg ccgaacttgt cctcaaacgc catgttcagc
     3121 tctaccgcat ccaggctgtc cgcctgcagg tcgtcggtca ggctggcatc catggttacc
     3181 ttgtcagcat cgcagttcag ggtgtcaaca aatacttctt tcaattcttc aaagttcata
     3241 ctacgtacct cacataattt aactaaataa attgacataa ttgtcaatga ggtcattata
     3301 gcacatttgc ttcgaatgtc aatattatta gataaaaata tttatctaat tgaagcgtca
     3361 ttgtgctcct cttccggtat tataaaagga tagtacacaa attatcccgc tacaggggca
     3421 gcccttgctg actactattt gaccactgct gcgtatcttc cctgtgaaat atccaatcgc
     3481 ctttaaatgg ttttatttac tttttctttt actattccag attattttct attctgcaac
     3541 atatttcgct ctaatgtgct atactaataa tatccaataa tgatggagga acttctatga
     3601 aacgcagcgg catcctactc caatacaatg cccccgtcac actgaccttt ttctttttgt
     3661 ccctcgcctc tctgatattg ggctacttaa caaacaactg gacaacctcg cacctgttta
     3721 gtgtgtatcg ctcgtcgatg acggatccac tattctatgt ccggttgttc ggtcatgtcc
     3781 ttggccacgg cggttggaat cactttataa acaatatgct cctcttcctt gttatcgctc
     3841 cacctttgga agaacgatat ggaagccgaa cacttctaag cggtatcctt atgaccgcgc
     3901 tggtatccgg catattgcag tgtgtgctgt tccccacttc tgcattgctt ggtgcatccg
     3961 gtatcgtatt catgctcatc atgctctcca gtttggccgg cagtaagaat ggaggtatcc
     4021 ctatcacctt c
//
LOCUS       DBMDSQ010000190         4026 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_5208_length_4026_cov_1.752357, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000190 DBMDSQ010000000
VERSION     DBMDSQ010000190.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 4026)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4026)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4026
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5208_length_4026_cov_1.752357"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            49..912
                     /gene="fba"
                     /locus_tag="ACZPAX_07275"
     CDS             49..912
                     /gene="fba"
                     /locus_tag="ACZPAX_07275"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016281851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0030388 - fructose 1,6-bisphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HIR1113739.1"
                     /translation="MLVNATNMLLKARDGHYAVGQFNINNLEWTKSVLLTAQEMNSPV
                     ILGVSEGAGKYMTGFKTVAAMVKAMDESLGITVPVALHLDHGTYEGAKACVAAGFTSI
                     MFDGSHYAIDENVAKTTELVALAHDHGLSIEAEVGSIGGEEDGVIGMGEVADPAECAK
                     IASLGIDFLAAGIGNIHGKYPANWKGLDFVALDKIHNATDNIPLVLHGGTGIPDEMIK
                     KAISLGVSKINVNTECQLSFADATRKYIEAGKDLEGKGFDPRKLLAPGAEAIKATVRE
                     KIKLFGSANKA"
     gene            1057..1557
                     /locus_tag="ACZPAX_07280"
     CDS             1057..1557
                     /locus_tag="ACZPAX_07280"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR1113740.1"
                     /translation="MNIPELDELYALYRADLYRYLCRLTRDSAAAEDLLSETFLRALK
                     GAVTFRGESGVKTWLFTIARNAWCESLRRQHPTVSTDELVTAQYLADDTLHTHTETRA
                     LLHRVRELLDGYGDRPRDIVLLRAQGYSYAEIAARLDITENSARVIEHRTRARLRADL
                     TKEGYE"
     gene            1554..2477
                     /locus_tag="ACZPAX_07285"
     CDS             1554..2477
                     /locus_tag="ACZPAX_07285"
                     /inference="COORDINATES: protein motif:HMM:NF051836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HIR1113741.1"
                     /translation="MTPEKISCGICRDLLPLVLDGVAAPESEAAVRAHLAACPECRAV
                     WQAECGDTPSPAPDILPDDARILCRLRRRVRGWLALALAAALLVALFAYTVNGDPISG
                     AWAQQRAIRYAEQRFPGQTFTAPKGSWYSYYFNYSVEVQSDQSPDTHFTVETRKWLWL
                     DDYGTTDAVTNGVTARQRMEQALTDKAQRTFDALSDRSDLDGTLQVELCVEPDADSSS
                     GYAPHTEHYSDVIYLDAPMDEAILDKVPSCLTLTIFWPTAATETDVQTVLREVKQLME
                     QSDLPMTYYHVTLLGSDGIVGSGVVAANAVG"
     gene            complement(2495..3361)
                     /locus_tag="ACZPAX_07290"
     CDS             complement(2495..3361)
                     /locus_tag="ACZPAX_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014793475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR1113742.1"
                     /translation="MIYLSGFRFPGAAQEANYMANSWRAKQTCYDSYYPFGLFEGRTL
                     PELDLLEITILYGGNGSGKTTLLNVMADALRLHRRAEYNRTPFFDDYVRLCEPRTMRP
                     VPAGSLILTSDDVFDYMLDLRALNDGVDMRREQMFADYNSLRCEKFQMHGMQDYDRLK
                     QVSAARRYSQSQMARRTLPANVRTRSNGESALAVFSERIRENALYLLDEPENSLSPAR
                     QLELAQFLHDSARFYNCQFVIATHSPFLLAMPGARVYDLDAEPVTTRKWTELENVRAT
                     WEFFQAHRDEFA"
     gene            3436..>4026
                     /locus_tag="ACZPAX_07295"
     CDS             3436..>4026
                     /locus_tag="ACZPAX_07295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HIR1113743.1"
                     /translation="MANGSIQIYTSAGQAAPLAGVALTVLDENGAALAHLTADADGFA
                     EAADLPAPDAAYSLDEANSAVRPYAIYSVEAVLDGWQTVVLNGVQVFDGQQTVARLSL
                     LPHGGIPSAARTAGGETDPDVVTIPPHTLFAGDGGSGPAPEELLPGDVLARVVVPKKI
                     TVHLGKPSANVRNVTVNFQSYIANVASSEVYPTWDGV"
BASE COUNT          790 a         1274 c         1175 g          787 t
ORIGIN      
        1 gacctcggta cgtcactact gataacgttt tacgtggagg tatgttctat gctggtaaat
       61 gctaccaata tgctgctgaa ggcccgcgat ggtcattacg cagtcggtca gttcaacatc
      121 aataacctgg agtggaccaa gagtgtcctg ctcaccgcac aggagatgaa ctctcccgtc
      181 atcctcggtg tttctgaggg tgctggcaaa tatatgactg gtttcaagac cgtggccgcc
      241 atggtcaagg ccatggacga gagcctgggc atcaccgttc ccgttgccct gcatctggac
      301 cacggcacct atgagggcgc taaggcctgc gtcgccgccg gtttcacctc tatcatgttc
      361 gacggcagcc actacgccat cgacgagaac gtcgccaaga ccaccgagct ggtcgccctg
      421 gcccatgacc acggcctgtc catcgaggct gaggtcggct ccatcggcgg cgaggaggac
      481 ggcgtcatcg gtatgggcga ggttgctgac cccgctgagt gcgccaagat cgcatccctg
      541 ggcatcgatt tcctggctgc cggtatcggc aacatccacg gcaagtaccc cgccaactgg
      601 aagggcctgg atttcgtcgc gctggacaag atccacaacg ccaccgacaa cattcctctg
      661 gtcctgcacg gcggcacggg cattcctgac gagatgatca agaaggccat ctccctgggc
      721 gtctccaaga tcaacgtcaa caccgagtgc cagctgagct ttgccgatgc tacccgcaag
      781 tacatcgagg ccggcaagga tctcgagggc aagggcttcg atccccgcaa gctgctcgcc
      841 cccggcgccg aggccatcaa ggctaccgtc cgcgagaaga tcaagctgtt cggctctgcc
      901 aacaaggctt gatttttcag ctgcctgaaa taagcatttc gcccgcaggt gtggttttcc
      961 accacgcctg cgggcgtttt tatttttgtg cggcttgtaa tattttgccg ctttgtgtac
     1021 tctaatgata taagagcaga aaggggggcg caccatatga atatacccga gttggatgaa
     1081 ctgtacgccc tctaccgcgc cgacctgtac cgctatctct gccgcctgac ccgcgactcc
     1141 gccgcggcgg aagacctgtt gagcgagaca ttcctgcggg cgctgaaggg cgcggtgacg
     1201 ttccgtggcg aaagcggcgt caagacctgg ctcttcacga ttgcccgaaa cgcctggtgc
     1261 gaaagcctgc ggcggcagca ccccaccgtc agcaccgacg aactggtgac cgcgcaatac
     1321 ttggccgatg acaccctgca tacccacact gagacccgcg ccctgctgca ccgcgtgcgc
     1381 gaactgctgg acggctacgg cgaccgcccc cgggacattg tactgctgcg ggcgcaaggg
     1441 tacagctacg ccgagattgc cgcgcggctg gacattaccg aaaactccgc ccgagtgatt
     1501 gaacaccgca cccgcgcccg gctgcgtgcg gatttgacga aggagggata cgaatgacgc
     1561 ccgagaaaat ttcctgcggc atttgccgcg atttgctgcc gctggtgctg gacggtgtgg
     1621 ccgcccccga gagtgaagcc gccgtgcgtg cccatctggc cgcctgcccc gaatgccgcg
     1681 cggtgtggca ggccgaatgc ggggatacgc cctcccccgc cccggatatc ctcccggatg
     1741 atgcaagaat tctgtgcaga ctgcggcggc gcgttcgcgg ctggctggca ctggctttgg
     1801 cggcggcgct gcttgttgcg ctgtttgcct acacggtcaa cggcgacccc atcagcggcg
     1861 cgtgggcgca gcaacgggcc atccgctatg ctgagcagcg ttttcccggc cagaccttta
     1921 ccgcgccgaa gggcagctgg tattcctact atttcaatta ttctgtcgag gtgcaatccg
     1981 accaaagccc tgatacccat ttcacagtgg agacgcgcaa gtggctgtgg ctggatgact
     2041 acggcaccac cgacgctgtg accaacggcg tgaccgcccg tcagcggatg gaacaagcgt
     2101 tgaccgacaa ggcccagcgg acgtttgacg ctctgagcga ccggtcggat ttggacggca
     2161 ccctccaagt ggaactctgc gtagagccag acgcggacag cagcagcggc tatgccccgc
     2221 atacggaaca ttacagcgat gtgatttatc ttgatgcccc gatggatgaa gcgattctgg
     2281 acaaagttcc ctcctgtctt accctcacca ttttctggcc gacggcggcg acggagacgg
     2341 atgtgcagac ggttttgcgg gaggtcaaac agctgatgga gcagtccgat ttgccgatga
     2401 cctactacca cgtcaccctc cttggcagcg acggcattgt gggttccggc gtggttgcgg
     2461 caaatgctgt tggctgaatt ttccaccaga cgctctatgc aaactcatcc ctatgcgcct
     2521 ggaaaaactc ccatgttgcc cttacattct ccaattccgt ccacttccgt gttgtgacag
     2581 gctcggcatc caaatcatac acccgcgccc cgggcatggc cagcaaaaac ggtgagtggg
     2641 tcgcgatgac aaattggcag ttataaaacc gcgccgaatc atgcaaaaac tgtgccaatt
     2701 ccaactgccg cgcgggcgaa agactgtttt ccggctcgtc cagcaggtag agcgcgttct
     2761 cgcggatgcg ctcactgaac actgccagcg cactttcgcc gttggagcgg gtgcgcacat
     2821 tggcgggcag cgtgcgtcgg gccatctggg actggctgta ccgccgcgct gcgctgacct
     2881 gcttgagccg gtcatagtcc tgcataccgt gcatctggaa cttttcacac cgcagcgaat
     2941 tataatctgc aaacatctgt tcgcggcgca tatctacgcc gtcattcagc gcccgcagat
     3001 ccaacatata gtcaaacaca tcatcactcg tcaagatcag gctgcctgcc ggcaccgggc
     3061 gcattgtgcg cggctcacac aggcgcacgt aatcgtcaaa gaacggcgtg cggttgtact
     3121 ctgcccggcg atgcagcctc aatgcgtcgg ccatcacatt taacagtgtc gtcttgccgc
     3181 tgccgttgcc gccgtacaaa atcgtgattt ccagcaaatc cagctccggc agcgtgcggc
     3241 cctcaaacag cccgaacgga tagtaggaat catagcaggt ctgctttgcc cgccagctgt
     3301 tcgccatgta gttggcctcc tgcgccgcgc cggggaaccg aaacccggaa agataaatca
     3361 tgcgcccgcc tccttttccc ctattgtagc acgttttcgc gccgctgcat agcgtgggac
     3421 aggaggtgtt ttgctatggc caatggcagt atccaaattt acacatcggc ggggcaggcg
     3481 gccccgctgg ccggtgtcgc cctgaccgtg ctggacgaga acggcgcggc gctcgcccac
     3541 ctgacggccg acgcggacgg ctttgccgaa gccgccgacc tgcccgcccc cgacgccgcg
     3601 tacagtctgg acgaggccaa ctcagcggtg cgcccctacg cgatttacag cgtagaggcc
     3661 gtactggacg gctggcaaac ggtcgtgctg aacggcgtgc aggtctttga cggccaacag
     3721 accgtggccc ggctgtcgct tttgccccac ggcggcatcc ccagcgccgc acggacagcg
     3781 ggcggcgaga cggatcctga tgtcgtcacc atcccgcccc acacgctgtt tgccggggac
     3841 ggcggcagcg gcccggcacc ggaggaactc ttacccggcg acgtcctggc ccgggtcgtc
     3901 gtgccgaaaa aaatcacggt gcatctgggg aaaccatccg ccaacgtcag aaatgtgacg
     3961 gtcaattttc agtcctacat cgccaacgtc gcgtccagtg aggtctaccc tacctgggat
     4021 ggcgtt
//
LOCUS       DBMDSQ010000191         4001 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_5242_length_4001_cov_1.880256, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000191 DBMDSQ010000000
VERSION     DBMDSQ010000191.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 4001)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4001)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4001
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5242_length_4001_cov_1.880256"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..867)
                     /gene="truB"
                     /locus_tag="ACZPAX_07300"
     CDS             complement(<1..867)
                     /gene="truB"
                     /locus_tag="ACZPAX_07300"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HIR1113744.1"
                     /translation="MQTPNGILPIDKPAGWTSFDVLAKLRGALGTRKLGHSGTLDPMA
                     TGVLAVFIGKATSAADRQLDHDKTYEATIRLGLRTDTGDVTGTTLETAPVTVGERELR
                     TMLSRFMGDRMQLPPMYSAVKINGQPLYKAARKGQTVERTPRPITIYNIEYLSSPAPD
                     EYTIRVACSKGTYIRVLAEEIGAALGVPATLAALRRTQAGVFNLKQCHTLPEILAAAE
                     SGELETNGWVLPIETVFAPLLALHVNEGVKAHLLNGCPTSHYKAADGRYRTYDENGAF
                     LGLATVEGGVLKV"
     gene            complement(929..1915)
                     /locus_tag="ACZPAX_07305"
     CDS             complement(929..1915)
                     /locus_tag="ACZPAX_07305"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015565886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HIR1113745.1"
                     /translation="MTQNVDRETVVSRLLAADDILILCHKNPDGDTIGSGTALCLALQ
                     QLGKNAAVLCSDPIPAMYDYMPITVFDGSFSPAFVVAVDVAGIQLFGERNNIRDYAEH
                     VNLCIDHHGSNSGYAYETLVDDHAAAAAELLTTLIPEMGAKITPEIAACLYTGVATDT
                     GCFRFSNTTAETHRAAAALIEAGADVEKLNERLFESRSHARVIAERMALESLEFYYDD
                     RCALICLTWDQIQAAGVAGAELEDLTSLPRSIEGVEVGLTLRQQKDGSFKISVRTGHD
                     TNACNIARRLGGGGHPRAAGCEISGNPDNAKHAILEEVKKELDRVDAEKEAQ"
     gene            complement(1946..2311)
                     /gene="rbfA"
                     /locus_tag="ACZPAX_07310"
     CDS             complement(1946..2311)
                     /gene="rbfA"
                     /locus_tag="ACZPAX_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005945412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HIR1113746.1"
                     /translation="MPTKNHGRMAQDMKRELIAIIGEMKDPRVNGGLLTVTRLDVTPD
                     LDQAKVYISVMGREGGPEPVVKALNKASGHVRTEVSRRMHIRKAPRFVFVKDEGAAYA
                     AHINKLLGELDIESDSDAE"
     gene            complement(2329..>4001)
                     /gene="infB"
                     /locus_tag="ACZPAX_07315"
     CDS             complement(2329..>4001)
                     /gene="infB"
                     /locus_tag="ACZPAX_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HIR1113747.1"
                     /translation="IAKFMQMGEMHAISDVVDFDTAALIAEEFHAKVEHEVHVSIEER
                     LFVQEEDNAADLVERPPVVVVMGHVDHGKTSILDAIRKTNVTKGEAGGITQAIGAYQV
                     KSNDSVITFLDTPGHEAFTSMRARGANMTDIAVLVVAADDGIMPQTIESINHAKAANV
                     KLIVAINKMDKPTANPERVKEQLTKYEIVPEDWGGDVACIPVSAVTGMGISDLLERIA
                     LEAEVMELKANPSRRGKGAVVEARLDKGQGPIATLLVQNGTLHKGDCLIAGTAVGRVR
                     TMRNDKGQEITEAGPSTPVEITGLTEVPEAGELFEAVEDERLARELADKRTAEAKEKQ
                     FAAYTKVTLDNLFDQMAANDMKELPIIVKADVQGSAEAVKQSLEKISNDEVRVRVIHA
                     GVGAISKSDVSLADASNAIIIGFNVRPDAVAKAEAEQTGVEMRMYRVIYDAINDVSDA
                     MKGMLAPKVREVALGEAQVRQVYKISSVGTVAGCRVTDGKITRDAQLRLVRDGIVICE
                     DSIASLKRFKDDAKEVAEGYECGITLAKFQDIKEGDVFECFKMEEYRD"
BASE COUNT          668 a         1211 c         1279 g          843 t
ORIGIN      
        1 gactttcagc acaccgccct cgacggtggc aagacccagg aacgcaccgt tttcgtcgta
       61 ggtgcgataa cggccatcgg cggccttata gtggctcgta gggcagccgt tgagcaggtg
      121 ggccttcacg ccctcattga catgcagggc gagcagcggg gcgaacaccg tttcaatcgg
      181 cagcacccag ccgttggtct caagttcccc gctctcggcg gcggccaaga tttccggcag
      241 cgtgtggcac tgtttcaggt tgaacacgcc cgcctgggtg cggcgcagcg ctgccagcgt
      301 ggcgggcacg ccgagggccg cgccgatctc ctcggccagg acgcggatgt aggtgccctt
      361 ggagcaggcc acgcggatgg tgtattcatc gggtgcgggg ctgctgaggt actcaatatt
      421 ataaatcgtg atggggcggg gcgtgcgctc cacggtctgg cccttgcggg cggccttata
      481 cagcggctgg ccgttgattt ttaccgcact gtacatcggc ggcagctgca tcctgtcgcc
      541 catgaaccgg gacagcatgg tgcgcagctc acgctccccc accgtgaccg gcgcggtttc
      601 cagcgtggtg cccgttacgt cgccggtgtc ggtgcgcagg cccaggcgga tggttgcctc
      661 ataggtttta tcgtggtcaa gctggcggtc ggcggcagaa gttgccttgc cgataaacac
      721 cgcgagcaca ccggtggcca tagggtctag tgttcccgaa tgtcccagct tgcgtgtgcc
      781 cagcgcgccg cgcagcttgg ccagcacatc aaagctggtc cagcctgcag gcttatcgat
      841 aggcagaatg ccgtttggcg tttgcatagc agggttcctt ttctgttgtt agggtttaaa
      901 aggctttccc ctagggggga aaaaggcttt attgcgcttc tttctccgcg tcgacgcggt
      961 ccagttcctt tttgacttcc tcgagaatgg catgtttggc gttgtccggg ttgccgctga
     1021 tctcacagcc tgcggcgcgg gggtggccgc cgccgcccag ccgccgtgca atgttgcaag
     1081 cgttggtgtc gtggccggtg cgcacgctga ttttaaaact gccgtccttc tgctggcgca
     1141 gggtcaggcc gacctcgacg ccctcaatgg agcggggcag actggtcaag tcttccagct
     1201 cggccccggc cacgcctgcg gcctggatct ggtcccaggt caggcagatg agcgcacagc
     1261 ggtcgtcgta gtaaaattcc aggctttcca gcgccatccg ctcggcaatg acccgcgcgt
     1321 gggagcggct ctcgaacagg cgctcgttga gcttttccac atcggcaccg gcctcgatga
     1381 gcgccgccgc cgcgcggtgg gtctccgccg tcgtgttgct gaaccggaag cagccggtgt
     1441 cggtcgccac gcccgtgtac aggcaggcgg cgatctccgg cgtgatcttc gcgcccatct
     1501 cggggatgag cgtcgtcagc agctcggcgg cggcagccgc gtggtcgtcc accagcgtct
     1561 cataggcata gccgctgttg gagccgtggt ggtcgatgca cagattgacg tgctcggcgt
     1621 aatcccggat gttgttgcgc tcgccaaaca actggatacc ggccacatcc accgccacga
     1681 cgaacgcagg gctaaagctg ccgtcaaaca ccgtgatggg catatagtcg tacatggccg
     1741 ggatggggtc gctgcacagc accgcggcgt ttttgcccag ctgctgcagg gcaaggcaga
     1801 gcgccgtgcc cgagccgatg gtatcgccat ccgggttttt atgacacaag atcaagatgt
     1861 catctgcagc aagcagacgc gaaacaacgg tctcgcgatc cacattctga gtcataggtt
     1921 cctcttttct aaccgggtgc agggtttact ctgcgtcaga gtcggactcg atgtccagct
     1981 cacccagcag cttgttgatg tgcgcggcat aggctgcgcc ttcatctttc acaaatacaa
     2041 agcgcggtgc cttgcggatg tgcatacggc ggctgacctc ggtgcggaca tggccggagg
     2101 ccttgttcag cgccttgact accggctcgg ggccgccctc gcggcccata acgctgatgt
     2161 agaccttggc ctggtcgagg tcaggggtca catccagtcg ggtcacggtc agcaaaccac
     2221 cgttcacacg ggggtccttc atctcgccga taatcgcaat cagctcacgc ttcatatcct
     2281 gggccatacg gccgtgattt ttggttggca tttagtttac ctctgcaatt agtcgcggta
     2341 ctcttccatc ttgaagcact cgaacacgtc gccttccttg atatcctgga acttggcaag
     2401 cgtgatgccg cactcgtagc cctcggccac ttccttggcg tcgtccttaa agcgcttcag
     2461 gctggcgatg gaatcctcgc agatgacgat gccgtcacgc accaggcgca gttgggcatc
     2521 gcgggtgatc ttgccgtcgg taacgcggca gcctgcaacg gtgccgacgc tggagatctt
     2581 gtagacctga cggacctgtg cttcgcccag ggcgacttcg cggaccttcg gggccagcat
     2641 acccttcata gcgtcggaaa catcgttgat ggcgtcgtag atgacgcggt acatacgcat
     2701 ctccacgccg gtctgctcgg cttccgcctt ggcaacggcg tcggggcgga cgttgaagcc
     2761 gatgatgatg gcgttggacg catcggccag agagacgtcg gacttggaaa tggcaccgac
     2821 acccgcatgg ataacgcgga cgcggacctc atcattggag atcttctcaa ggctttgctt
     2881 gacggcctcg gcggagccct ggacgtcggc cttgacgatg atgggcagtt ccttcatatc
     2941 gttggcagcc atctggtcaa acaggttatc cagcgtaacc ttggtgtagg cggcaaactg
     3001 cttttccttg gcctcggcgg tacgcttgtc agccagctca cgggccaggc gctcgtcctc
     3061 aacggcctcg aacagctcac cggcctcggg cacttcggtc aggccggtga tctccacggg
     3121 ggtggagggg ccggcctcgg tgatttcctg gcccttgtcg ttgcgcatgg tacgcacacg
     3181 gccaacggct gtaccggcga tcaggcagtc gcccttatgc agggtaccgt tctgaaccag
     3241 cagggtcgcg atggggccct gacccttgtc caggcgggcc tcgacaacag cgcccttgcc
     3301 gcggcggctc gggttggctt tcagttccat gacctcagcc tcaagggcga tgcgctccag
     3361 caggtcgctg atgcccatgc cggtgacggc agagacgggg atgcaggcga cgtcgccgcc
     3421 ccaatcctcg gggacgatct cgtacttggt cagctgctcc ttgacgcgct ccggattggc
     3481 ggtgggctta tccatcttgt tgatggcgac gatcagcttg acgttggcgg ccttggcatg
     3541 gttgatagac tcgatagtct ggggcatgat gccgtcatcg gccgcgacga ccagaaccgc
     3601 gatatcggtc atgttcgcgc cgcgggcacg catcgaggtg aaggcctcgt ggcccggggt
     3661 gtccaggaag gtgatgacgc tgtcattgct cttgacctgg taggcgccga tggcctgggt
     3721 gataccgccg gcctcaccct tggtgacatt ggtcttgcgg atggcgtcca ggatggaggt
     3781 tttaccgtgg tcaacgtggc ccatgacgac gacgaccggc ggacgctcga cgaggtctgc
     3841 ggcgttgtcc tcttcctgca cgaagaggcg ctcctcgatg gagacatgga cctcgtgctc
     3901 gaccttggcg tggaactcct cggcaatcag ggcggcggtg tcgaaatcga cgacgtcaga
     3961 gatggcgtgc atctcgccca tctgcatgaa cttggcaatg a
//
LOCUS       DBMDSQ010000192         3989 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_5261_length_3989_cov_1.710391, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000192 DBMDSQ010000000
VERSION     DBMDSQ010000192.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 3989)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3989)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3989
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5261_length_3989_cov_1.710391"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(9..230)
                     /locus_tag="ACZPAX_07320"
     CDS             complement(9..230)
                     /locus_tag="ACZPAX_07320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113748.1"
                     /translation="MKLQDGGAFTPPLGATIHMQQKIPELTFEDFGADEGIFPQAGEL
                     SAGQFAPALRRRRPVQILFTARPNEKPRT"
     gene            482..1744
                     /gene="sstT"
                     /locus_tag="ACZPAX_07325"
     CDS             482..1744
                     /gene="sstT"
                     /locus_tag="ACZPAX_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006735332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HIR1113749.1"
                     /translation="MNQLTKAAKAVATRYNQTSLILRITVGLVVGAALALICPGAAWI
                     EELGNLFVGALKGIAPVLVFVIVASALAQGSSKLDRRFGTVVWLYMLTTFVAAALSVV
                     TSKLFPQTLVLAEAATADVIPQGLGDVMHTLLANIVSNPVASIMNGNYIGILMWACLF
                     GLAMKRLGSDTTKNFMANTADAVSTIVRWVINMAPFGIMGLVFTNVADNGLSIFTQYG
                     RLLLLLVGTMLFMALVINPFIIFIYLHRNPYPLVFRCLRESGLTAFFTRSSAANIPVN
                     MALCEKLGLDKDIYSVSIPLGATINMDGAAITITIMTLAAANTLGMDVSVPAAVLLSV
                     MSALGACGASGVAGGSLLLIPMACSLFGISNDIAMQVVGVGFIIGVIQDSVETALNSA
                     GDVEFAATAEYHQWLKESKPLPAFMGGK"
     gene            complement(2231..2317)
                     /locus_tag="ACZPAX_07330"
     tRNA            complement(2231..2317)
                     /locus_tag="ACZPAX_07330"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2281..2283),aa:Leu,seq:cag)
     gene            complement(2468..3886)
                     /gene="glgA"
                     /locus_tag="ACZPAX_07335"
     CDS             complement(2468..3886)
                     /gene="glgA"
                     /locus_tag="ACZPAX_07335"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015537347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - alpha-1,4-glucan
                     glucosyltransferase (UDP-glucose donor) activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HIR1113750.1"
                     /translation="MKILYAASEAAPFAKSGGLADVAGALPKALVRDGIDARVVMPLY
                     GDLKFKDTLTYVTNFSVPVGWRSQYCGLFKAERDGVVYYFIDNEYYFKRSGLYGFYDD
                     GERFAFFSRAILEMLFYTDFEPDIINCNDWQTALTPVYLNLYYRHLDKFSRIKTVFTI
                     HNIAYQGKYGLDILEDTCGIGARDAHVVEYDGCANFMKGAIECADKVTTVSPTYAQEI
                     LDPWFSHGLDGLLRQKQYKLCGILNGIDVDVFNPATDPDIAKNYDAETFQDGKAVCKE
                     HLQDEFGLHKDGSPIMAMVTRLVGHKGVDLVQAIAEGLLQQGIELVILGSGESQYENF
                     FNELCARNPGRVGVYIGFNAKLAQEIYAGADIFLMPSRSEPCGLAQMVSCRYGTIPVI
                     RETGGLKDSIRDSGDGYGNGFTFANYNAHELYEACWRANEGYWQKDGWPVLVKRAMEC
                     DFSWSNSAKSYEGLYNEVVNLW"
BASE COUNT          834 a         1129 c         1133 g          893 t
ORIGIN      
        1 ccccgaactt aggttcgggg cttttcgttt ggtcttgcgg tgaagaggat ttgaacaggg
       61 cgacggcgcc gcagcgccgg ggcaaactgt cctgcggaca gttcgccagc ctgcgggaaa
      121 atcccttcat ccgcaccaaa atcctcgaac gttagttcgg ggattttttg ctgcatatga
      181 attgtggcgc caagcggcgg ggtgaaggca cccccgtcct gtaatttcac agtaaactgt
      241 ccgcaccacg aatacaccta tttcgctcat aaaaacaaat tttaatattt ttttacatcg
      301 tcccatcgca aaaaacgacg ggtttctctg gctggtacaa ctgtccgtat cttgcaatct
      361 gcacattttg cggtataatg tggattatta cgcaaaagcg gtctgtgcgg gcggcttttg
      421 cacgctgggc gcaccctgca agccgtgccc ggcgcagtat aaaaagggaa gaggaatttg
      481 tatgaaccaa ctcacgaaag cggccaaagc ggtggctacc cggtataatc agaccagcct
      541 gattctgcgc atcaccgtcg gcctagtcgt cggtgcggcg ctggcgctga tttgccccgg
      601 cgcggcatgg atcgaggaac ttggcaacct gtttgtcggc gcactgaaag gcattgcccc
      661 ggtgctggtt ttcgtcattg tcgccagtgc gctggcgcag ggctcgtcca agctggaccg
      721 ccgcttcggc acggtcgttt ggctgtatat gctcaccacc tttgtggcgg cggcgctcag
      781 cgtcgtgacc agcaagctgt tcccccagac gctggtgctg gccgaggctg ctactgcgga
      841 cgtgattccc cagggcttgg gtgacgtcat gcacacgttg ctggcgaaca tcgtctccaa
      901 cccggtggcg tccatcatga acggcaacta catcggcatc ctgatgtggg cctgcctgtt
      961 tggcctggct atgaagcgcc tgggcagcga caccaccaaa aactttatgg ccaacacggc
     1021 ggacgcggtt tccaccattg tgcgctgggt catcaacatg gctcccttcg gcatcatggg
     1081 cctggtgttt accaacgtgg cggacaacgg cctgtccatc tttacccagt acggccgtct
     1141 gctgctgttg ctggtgggca cgatgctgtt catggcgctg gtcatcaacc cgttcatcat
     1201 cttcatctac ctgcaccgca acccctaccc gctggtgttc cgctgcctgc gtgaaagcgg
     1261 cctgaccgcc ttcttcaccc gcagctctgc cgccaacatc ccggtcaata tggccttgtg
     1321 tgagaagctg gggctggaca aggacattta ctctgtctcc atccctctgg gcgcgaccat
     1381 caacatggac ggtgcggcca tcaccattac cattatgacg ctggctgcag ccaacacgct
     1441 gggcatggat gttagtgtgc ctgccgccgt cctgctgtcc gtcatgtccg cactgggcgc
     1501 gtgcggcgct tccggcgtcg cgggtggctc gctgttgctc atccctatgg catgctcgct
     1561 gttcggcatc tccaatgata tcgctatgca ggtcgtcggc gtcggtttca tcatcggcgt
     1621 cattcaggac tccgtcgaga ctgcgctgaa ctccgccggt gacgtggagt tcgccgccac
     1681 cgccgagtat caccagtggc tcaaggagag caaacctctc ccggcattta tgggtggaaa
     1741 gtaagagata cattaaataa gtacagcccc ggtttcgtca gcaaaaccgg ggctgcattt
     1801 atgtcagaga aaaaaaggaa agtctcgcgg cggcagagga ttaccgccgc ctgctgcccc
     1861 tactttgtta ttcgaaattt gtgccgccct ttttgggaaa aataaaattc aataatcaac
     1921 agtagatttt gttctatgta cgtggagcgg taatcagtat aatatctgcg gggtgcttgg
     1981 gggaacgctc tgaatgacca gcatcttttg tatcgcggtg cggtaaaagt tcggtacggc
     2041 aaaaagaaaa gacctaagaa caaacgttct taggtctttt tctttggtgc ggatgaaggg
     2101 attttcccgc aggctggcga actgtccgca ggacagtttg ccccggcgct gcagcgccgt
     2161 cgccctgttc aaatcctctt caccgcaaga ccaaacgaaa agccccgaac ctaagttcgg
     2221 ggcttccgtt tggtgcggat gaagggattt gaacccacac tcttttaagg gaactagaac
     2281 ctgaatctag cgcgtctgcc agttccgcca catccgcata ttctgttggc caccgtgttc
     2341 cggcgacgtg tattattata gccaaacgtc acgtaaatgt caagcatttt tgcagaaaaa
     2401 tatcactttt tcctacaaaa aatgcagccc tgcgcttttg gacgcagggc cgcattttat
     2461 ttaccgatta ccagaggttg acgacctcgt tatacaggcc ctcatagctc ttggcagagt
     2521 tggaccagct gaaatcacac tccatggcgc gcttgaccag cacaggccag ccatccttct
     2581 gccagtagcc ctcattggca cgccagcagg cttcgtacag ctcgtgtgcg ttgtagttgg
     2641 caaaggtgaa gccgttgccg tagccgtcgc cggagtcgcg gatagagtcc ttcaggccgc
     2701 cggtctcgcg gatgacagga atggtgccgt aacggcagga gaccatctgt gccaggccgc
     2761 agggttcaga gcgggacggc atcaggaaga tatccgcacc ggcgtagatt tcctgcgcca
     2821 gcttggcgtt gaagccgatg taaacgccga cacggcccgg gttacgggcg cacagctcgt
     2881 tgaagaagtt ctcgtactgg ctctcgccgg aaccgaggat gaccagctcg atgccctgct
     2941 gcagcagacc ctcggcgatg gcctgaacca ggtcaacacc cttatggccg accaggcggg
     3001 tgaccatagc catgatcggg ctgccgtcct tatgcaggcc aaactcgtcc tgcaggtgtt
     3061 ccttgcagac agccttgccg tcctggaagg tctcggcgtc gtagttcttg gcaatgtcgg
     3121 ggtcggtggc ggggttgaac acatcgacgt cgatgccgtt cagaatgccg cacagcttat
     3181 actgcttctg gcgcagcagg ccgtccagac catggctgaa ccaggggtcg agaatttcct
     3241 gtgcataggt ggggctgacg gtcgtgacct tatcggcaca ctcgattgca cccttcatga
     3301 agttggcaca gccgtcgtac tccaccacat gggcgtcgcg ggcaccgatg ccgcaggtat
     3361 cctcgaggat atccaggccg tacttgccct ggtaggcaat gttatggatg gtgaacaccg
     3421 tcttgatgcg gctgaactta tccagatggc ggtaatacag gttcagatag accggggtca
     3481 gcgcggtctg ccagtcgttg cagttgatga tatccggctc gaaatcggtg tagaacagca
     3541 tttcgaggat ggcgcgggag aagaaggcaa agcgctcgcc gtcatcgtag aagccgtaca
     3601 ggccgctgcg tttgaagtaa tactcgttgt cgataaagta gtagacaacg ccgtcgcgct
     3661 cggccttgaa caggccgcag tactggctgc gccagcccac aggcacgctg aaattggtga
     3721 cataggtcag cgtatccttg aatttcaaat cgccgtacag cggcataacg acgcgggcgt
     3781 cgatgccatc gcgaaccagc gccttgggca gagcgcccgc aacgtcggcc aggccgccgg
     3841 acttggcaaa gggagcagcc tcgctggctg catacaggat tttcataagc acctccgtgc
     3901 gaatttcaaa aaatgagggc gagggaaaac aggctctccc ccgccctctc atcaaagccg
     3961 actccgggga gaaggagaac agcctctat
//
LOCUS       DBMDSQ010000193         3925 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_5351_length_3925_cov_1.881799, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000193 DBMDSQ010000000
VERSION     DBMDSQ010000193.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 3925)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3925)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3925
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5351_length_3925_cov_1.881799"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="ACZPAX_07340"
     CDS             complement(<1..219)
                     /locus_tag="ACZPAX_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HIR1113751.1"
                     /translation="MNRNYSNISYAESPFGTLSEFMSKSFGWMFAGLLVTFGVGMGAV
                     ASGLIFPIINSGLFIFTSIAELVMVFVLS"
     gene            313..1392
                     /gene="hemW"
                     /locus_tag="ACZPAX_07345"
     CDS             313..1392
                     /gene="hemW"
                     /locus_tag="ACZPAX_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HIR1113752.1"
                     /translation="MSKKEPFGIYIHIPYCKAKCRYCDFYSAPGARGVPQSYVDALLR
                     ELARNTRRPDTVYFGGGTPGLLSPAQVDALIQAAAPLPGAEITLEANPDIVTSETLRG
                     FRAAGVTRISFGVQSADDAQLRRLGRTHTAAGAAQALAWAREAGFDEICGDIMLALPE
                     YTNAEFDRTLALLRDGGCTHISAYLLKVEPGTAFYRSPPAGLPDGDAAADFYLYAVQQ
                     LEAAGYRQYEISNFARPGHEGRHNLLYWNCNDYWGIGPAAHSCVDNVRRFWPGDVQGF
                     IDGTIHEEIEGDCTVEDYLLMQLRLVSGLDIAEYERRGGRFTAAQRAFMRQCVGHEYA
                     TLDGNVFRLTPAGMVVQNAILAELM"
     gene            complement(1440..2003)
                     /locus_tag="ACZPAX_07350"
     CDS             complement(1440..2003)
                     /locus_tag="ACZPAX_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015565902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR1113753.1"
                     /translation="MSIEIENLCKKFGATPVLQNFSWTVDRPMVLMGRSGCGKTTLLR
                     ILLGLETADSGTVTGVETPAAVFQEDRLCPQLTAAVNLTLTGHGLSPQEAERELRGLG
                     FTDAELALPVARLSGGQKRRAALLRALLCKDAATLLLDEPFTGMDAELVADAVAATKR
                     LAGARPTILVTHDRTAADLLGWPVKEV"
     gene            complement(2000..2818)
                     /locus_tag="ACZPAX_07355"
     CDS             complement(2000..2818)
                     /locus_tag="ACZPAX_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005920396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1113754.1"
                     /translation="MQAKSDLIPSCRGGCEHRPLQTPLWQKAAAILVWILIWQLAAMG
                     LGHGGLFLATPLQTLGALAQLAPTAAFWQRIVFSALRIVAGFLLAAAGGLLLGAAGAR
                     WHWVRVFIDPAMQLIRAMPVASFVILALLWVRSANLSVVVSFTHVLPVVYAGVLGGIA
                     DTDPKLLEMAKVYRLPLSARLRYIWLPGVFPSFCESCIAAMGMCWKSGVSAEVIGLPD
                     HSVGDALYRAKITLSTPDVFAWTLVIVLLSAVLSAVAAWGLRTVKARLCGEVRT"
     gene            complement(2808..3878)
                     /locus_tag="ACZPAX_07360"
     CDS             complement(2808..3878)
                     /locus_tag="ACZPAX_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011459725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR1113755.1"
                     /translation="MKLKRVFAAVTAFTLCASLAGCGATAAVSESAVESVAESTVESA
                     AESAVEAEPATDESADTDAAEAALRIAGLKGPTTMGLVNLMADEVAENYNFTMYGAAD
                     EIVPLLVKGELDAAAVPANLAATLYNKTNGAVEVACINTLGVLYIVENGETVNSVEDL
                     KGQTIVTTGKGATPEYVLRYVLSENGIDPDSDVTIEYCSEATEALSKVQAGEATIAML
                     PQPFVTSALSQVEGLRVALDMNEEWQKVAGSKLVTGVLVVRKDAVENDPEAFTAFMDG
                     YAASVEAANNDLEGTAALCEEYGVIAKAALAQKALPQCNIVFETGNEMKTDLETYFNV
                     LYAADPASVGGTLPADDFYYAG"
BASE COUNT          807 a         1203 c         1211 g          704 t
ORIGIN      
        1 ggacaggaca aacaccatga ccagctctgc gatggaggta aagatgaaca ggccggagtt
       61 gataatcggg aaaatcaatc cggacgcaac cgcgcccatg ccgacgccga acgtgacaag
      121 caggcctgca aacatccagc cgaagctctt ggacataaac tcggaaagcg taccaaaggg
      181 gctttccgca tacgaaatat ttgaataatt gcgattcata gttgcaatct cctttcttta
      241 tgatgtatta tacaggtatt tgcacagcaa atcatcacta aattgtgaaa ttcccaaaac
      301 aaaaggtgca ttatgtccaa aaaagagccg tttggtattt acattcatat tccctactgc
      361 aaggcaaaat gccggtactg cgatttttac tcggccccag gtgcacgcgg cgtgccgcag
      421 agctacgtag acgcactgct gcgggagctg gcgaggaaca cccgccgccc cgatactgtg
      481 tatttcggcg gcggtacgcc cgggctgctg tcgcccgcac aggtcgatgc tttgattcaa
      541 gccgctgccc ccttgcccgg cgcagaaatc accttggagg cgaatcccga catcgtaacg
      601 tccgagacgc tgcgcgggtt cagggccgcg ggcgtaacac gcatcagctt tggtgtgcag
      661 agtgccgacg atgcccagct gcgccggtta ggccgcaccc acacggccgc cggggctgcg
      721 caggcgctgg cttgggcgcg ggaggccggg tttgacgaga tctgcggcga cattatgctg
      781 gcgctgcccg agtacacgaa cgccgagttt gaccgcacgc tggcgttgct gcgcgacggc
      841 ggctgcacgc atatctcggc ctatttatta aaggtagaac cgggcacagc cttttaccgc
      901 agcccgcctg caggcctgcc agacggcgat gcggcggcgg atttttacct gtacgccgtg
      961 cagcagttgg aggctgcggg ctaccgccaa tacgaaatca gcaattttgc ccgcccgggc
     1021 cacgaagggc ggcacaattt gttatattgg aactgcaacg actactgggg catcggcccg
     1081 gcagcccaca gctgcgtgga caatgtgcgg cggttctggc ccggggatgt gcaggggttt
     1141 attgacggca cgatccacga ggaaatcgag ggcgactgca cagtcgagga ttatctgctg
     1201 atgcagctac ggctggtcag cggactggat atcgccgagt acgagcggcg cggcggacgg
     1261 ttcacggcag cgcagcgggc gttcatgcgg cagtgcgtgg gccacgagta tgcaacgctg
     1321 gatggcaatg tgtttcgcct gacccccgcc gggatggtgg tacagaacgc gatattggcg
     1381 gaattgatgt gaaaaagaaa agcacgccca cgcgatagtt ttcgcgcggg cgtgcttttt
     1441 tatacttctt tcaccggcca gcccagcagg tcggcggcgg tgcggtcatg ggtgaccaaa
     1501 atcgtgggcc gcgcccctgc cagccgcttt gtggcggcga cagcatcggc gacaagctcg
     1561 gcgtccatgc cggtgaaggg ttcatccaac agcagcgtgg cagcatcctt gcagagcagc
     1621 gcccgcagca gggcagcgcg gcgcttctgc ccgccggaga gccgtgcaac gggcaaagcc
     1681 agctcggcat cggtaaagcc caggccgcgc agctcccgct ctgcctcctg cggtgacaga
     1741 ccatggcctg tcagcgtcaa gttcacggcg gcggtcagct gcgggcagag gcgatcctcc
     1801 tgaaagacgg cagcgggcgt ttccacgcct gtgaccgtgc cggagtcagc cgtttccaac
     1861 cccagcagaa tgcgcagcag cgtcgtttta ccacaaccgg accggcccat cagcaccata
     1921 gggcggtcga ccgtccagct gaaattctgc aaaaccggcg tggcgccaaa cttcttgcag
     1981 agattttcta tctcaatgct catgtgcgca cctccccgca gaggcgggct ttcaccgtgc
     2041 gcaggcccca ggccgctacg gcggacagca ccgccgacag cagcacgata accagcgtcc
     2101 aggcaaacac atccggtgtg gacagcgtga ttttggcgcg gtagagcgcg tcgccaacgc
     2161 tgtgatccgg cagaccgatg acctccgccg agacgccgct cttccagcac atgcccatgg
     2221 cggcgatgca gctttcgcag aacgacggga aaacgcccgg cagccagatg taccgcagcc
     2281 gtgcggacag cggcagacgg tagactttcg ccatttccag cagcttcggg tcggtgtcgg
     2341 cgatgccgcc cagcacaccc gcgtagacca ccggcagcac atgggtaaag ctgacaacga
     2401 ccgaaaggtt cgcgctgcgc acccagagca gggccagaat gacaaagctg gcgacaggca
     2461 tggcgcggat cagctgcatg gccgggtcaa tgaacacccg cacccaatgc cagcgcgccc
     2521 ccgccgcacc aagaagcagg ccccccgctg ctgccagcag gaagcccgcc actatgcgca
     2581 gcgcactgaa tacgatgcgc tgccagaacg cagccgtggg tgccagctgc gccagcgccc
     2641 ccagcgtttg caaaggcgta gccagaaaca gcccgccgtg ccccagcccc atggcggcaa
     2701 gctgccaaat cagaatccac acgaggatag cggcggcttt ttgccacagc ggagtttgta
     2761 ggggtcgatg ctcgcatccg cccctgcaag aggggatcaa atcagattta gcctgcatag
     2821 tagaagtcgt cggcaggcag ggtgccgccc acactggcgg ggtcagcggc gtacagaacg
     2881 ttgaagtacg tctcgaggtc ggtcttcatc tcattgccgg tctcaaagac gatgttgcac
     2941 tgaggcagcg ccttctgggc cagagcggcc ttggcaatga cgccgtactc ctcgcacagg
     3001 gcagcggtgc cttccaggtc attgtttgcg gcctcgacgc tggcggcata gccgtccatg
     3061 aaggcagtga acgcctcggg gtcattctcg acggcatcct tgcggacgac cagaacgccg
     3121 gtgacgagct tggaaccggc caccttctgc cattcctcgt tcatgtccag ggcaacgcgc
     3181 aggccctcga cctgggacag agcgctggtg acgaagggct ggggcagcat ggcaatggtc
     3241 gcctcaccgg cctggacttt gctcagggcc tcggtggcct cgctgcaata ctcgatggtg
     3301 acgtcgctgt cgggatcgat gccgttctcg ctcaggacat agcgcaggac gtactcaggc
     3361 gttgctccct tgccagtggt gacgatggtc tggcctttca ggtcctcgac cgaattgaca
     3421 gtctcgccgt tctcgacgat gtagagcacg cccagcgtgt tgatgcaggc gacttcgact
     3481 gcaccgttgg tcttgttata cagggtcgca gccaggttgg cgggcacagc ggcggcgtcc
     3541 aactcgccct tgaccagcag cgggacgatc tcatcagccg caccgtacat cgtgaaattg
     3601 tagttctcgg caacctcatc ggccatcagg ttgaccaggc ccatcgtggt ggggcctttc
     3661 agaccggcaa tgcgcagggc agcctcggcg gcatcggtgt cggcggactc gtcggtggca
     3721 ggctcggcct cgacggcgga ttcagcggca gattccacgg tggactcggc cacggattcc
     3781 acagcggact cgctgacagc ggcggtagcg ccgcagccag ccagagatgc acagagagta
     3841 aacgccgtaa cagcggcgaa aacacgtttc aacttcataa aaatcttatc ctccttgttg
     3901 cttttgcggc cctactatac tataa
//
LOCUS       DBMDSQ010000194         3909 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_5372_length_3909_cov_1.783876, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000194 DBMDSQ010000000
VERSION     DBMDSQ010000194.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 3909)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3909)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3909
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5372_length_3909_cov_1.783876"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="ACZPAX_07365"
     CDS             <1..308
                     /locus_tag="ACZPAX_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HIR1113756.1"
                     /translation="VTAGFLSLCGGALALAVYLLLLHLPGVSGYIFCTCGALAHNLGQ
                     LMGAAILLSSGMALAYAPVLLAAGLIVGGISCVMTKALFTALPSSAGQQRKGSPFKN"
     gene            429..965
                     /locus_tag="ACZPAX_07370"
     CDS             429..965
                     /locus_tag="ACZPAX_07370"
                     /EC_number="1.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002609625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH peroxidase"
                     /protein_id="HIR1113757.1"
                     /translation="MTKYVCTVCGYIAEGSIPEFCPVCKAPASKFIEKKDNTYVTEHV
                     VGIAKDVPEEIKQGLRENFNGECSEVGMYLAMSRVADREGYPEIAEAWKRYAFEEAEH
                     ASKFAELLGEVLTDSTKKNLQMRVDAECGACAGKMDLAKKAKELNLDAIHDTVHEMAK
                     DEARHGRGMQGLLDRYFK"
     gene            1045..1674
                     /locus_tag="ACZPAX_07375"
     CDS             1045..1674
                     /locus_tag="ACZPAX_07375"
                     /inference="COORDINATES: protein motif:HMM:NF021101.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YunB"
                     /protein_id="HIR1113758.1"
                     /translation="MRRCSVKAMRRAQLRGVLWLLAASLLAGGLWLDRYVNTVIKPTL
                     HELAEYEARAATVQAVNRAVAAALAADASLTDALFVQSGGIVSLDAAAAGAAQLRLVS
                     AVQEEMNVLPEEDFTIPFGSLTNNSLLSGLGPGWRMQVQPKGYAEGHIREATESLSIN
                     TTRYSAVLQLSVTVNMILDGSTATLTVYADYPLASVLIGGDTPSAYAGI"
     gene            1744..3735
                     /gene="ligA"
                     /locus_tag="ACZPAX_07380"
     CDS             1744..3735
                     /gene="ligA"
                     /locus_tag="ACZPAX_07380"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005942022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HIR1113759.1"
                     /translation="MELEQARKRAEELRVIIEKNNRLYYDQDAPELEDFEYDALNREL
                     KQIEAEYPELVTASSPTQHVGGTASSKFSKVTHAVKMESLQDAFSFDELREFDTRVRE
                     AGIEPEYVVEAKIDGLSVSLEYRMGQLVRGSTRGDGVVGEDVTENIMTIKDIPHELPD
                     APDFLEVRGEVYMPHSAFFKLKEQQELEDKMPFKNPRNAAAGSLRQKDSKITAERGLS
                     IFVFNLQQCEGRTFKTHHETLDYIKTLGFPVSPRYSVFENIEDAIKEIESIGEARGTL
                     EFDIDGAVIKVNDLAARRTLGSTNKFPRWAIAFKYPPEVKESVVRDIEVTVGRTGVLT
                     PTAVFDPIFLAGTSVSRASLHNGDIIANLGVGIGDTIKVRKAGDIIPEVIGVSARLPG
                     SKTFTMPTVCPSCGAPVVHLQDETALRCVNPECPAQSLRNLIHFASRNAMAIDGLGEA
                     VAVQLIDKGLVSTVADLYTLTEEQLLTLDKFKKKSVQNLLNAIEGSKRNNLDKLIFGL
                     GIRNIGDKAAALLGEHFGSMDALRQATAEQMCEIDGFGEVMAQSVLEFFAKDGTTDLL
                     RRLADDGVNMQWTGEKKGTALAGMTLVVTGTLPTLSRQEAEALITQNGGKAAGSVSKK
                     TSYVVAGEAAGSKLTKAQTLGIPVLDEAGLYRLIEENDQ"
BASE COUNT          873 a         1111 c         1162 g          763 t
ORIGIN      
        1 gcgtgaccgc tggttttctt tccttatgcg gcggggcgct ggcgctggcc gtgtatcttt
       61 tgctgttgca tctgcccggg gtcagcgggt acatattctg tacctgtggg gcgctggcgc
      121 acaacctcgg ccagctgatg ggggcagcca tcctcctgtc cagcggcatg gcgcttgcct
      181 atgcgccggt gctgctggcg gccgggctga tcgttggcgg catcagctgc gtgatgacca
      241 aagcactgtt caccgcgctg cccagttccg ccgggcagca gcggaagggg agtcccttta
      301 aaaattaaga ttatttctta aaaactacaa aattgctatt gacaaacctg taaacgtatc
      361 gtataatata gccacagaca caggaatgtg tcctaggagc tattacaaaa taacaggagg
      421 caattcttat gacgaagtac gtttgcaccg tttgtggtta tatcgctgag ggttctattc
      481 ccgagttctg cccggtctgc aaggccccgg catccaaatt tattgagaag aaggacaaca
      541 cctacgtgac cgagcatgtc gtcggcatcg ccaaggatgt tcccgaggaa atcaagcagg
      601 gtctgcgcga gaacttcaac ggcgaatgca gcgaggtcgg tatgtacctg gctatgagcc
      661 gcgtcgctga ccgcgagggc taccccgaga ttgccgaggc ttggaagcgc tacgctttcg
      721 aggaggccga gcacgcttcc aagtttgctg agctgctggg tgaggtcctg accgacagca
      781 ccaagaagaa cctgcagatg cgtgttgacg ccgagtgtgg cgcctgcgcc ggcaagatgg
      841 accttgccaa gaaggccaag gagctgaacc tcgatgccat ccatgacacc gtccacgaga
      901 tggcaaagga cgaggcccgt catggccgcg gtatgcaggg cctgctggac cgctacttca
      961 aataatcata cctaaagcac actgcgcccc ggagtttgaa cgctccgggg cgtttttgca
     1021 taggatacgc cggggggtgg tgccatgcgg cggtgcagtg taaaagccat gcggcgggcg
     1081 cagctgcgcg gggttttgtg gctgctggcg gcgtctctgc tggcgggagg gctgtggctg
     1141 gaccgctatg tcaacacagt catcaaaccc actctgcacg agctggccga atacgaagcc
     1201 cgcgcagcca ccgtgcaggc cgtcaaccga gccgtggcag cggcgctcgc agcggatgcg
     1261 tcccttaccg atgcgctgtt tgtgcagagc ggcggcatcg tcagtctgga cgcagcagcc
     1321 gccggggccg cgcagctgcg ccttgtcagc gccgtgcagg aggaaatgaa cgttttgccg
     1381 gaggaagatt tcacgattcc tttcggcagc ctgacgaaca actcactgct cagcggcctt
     1441 ggcccgggct ggcggatgca ggtacagccc aagggctacg ctgaggggca tatccgggag
     1501 gcgacggagt ctttatccat caataccaca cgatacagcg cagttttgca gctatccgtc
     1561 accgtcaata tgattctgga cggcagcacc gccacgctga ccgtatacgc cgactaccca
     1621 ttggcaagtg tgctgatcgg cggcgatacc ccgagcgcct acgccgggat ttgatttgat
     1681 tgttgaagtt tctctgtgca gtgtggtata ctaaagtgat accaacaaac ggagtggaac
     1741 agtatggaac ttgagcaagc ccgcaaacgc gccgaagaac tgcgcgtcat catagaaaaa
     1801 aacaaccgcc tttattacga ccaggacgcc cccgagctgg aagatttcga gtacgacgcc
     1861 ctgaaccggg agttaaagca aatcgaggct gaataccctg agctggtcac agcgtcctcg
     1921 cccacacagc atgtcggcgg cacggccagc agcaaattca gcaaggtaac gcacgccgtc
     1981 aagatggaga gtttgcagga cgcattctcg tttgacgagc tgcgggagtt tgacacccgc
     2041 gtgcgcgagg ctggcatcga gccggagtat gtcgtcgagg cgaaaatcga tggcctttcg
     2101 gtcagccttg aataccgcat gggccagctc gtgcgcggct cgacccgcgg cgacggcgtt
     2161 gtgggcgagg atgtgaccga gaacatcatg accatcaaag acatcccgca cgaactgccc
     2221 gacgcgccgg actttttgga ggtgcgcggc gaagtctata tgccgcactc ggcattcttt
     2281 aaactgaaag aacagcagga gcttgaggac aaaatgccct tcaaaaatcc ccgcaatgcg
     2341 gcggccggtt ctctgcggca gaaggattcc aaaatcacgg cagaacgcgg attgtccatc
     2401 tttgtgttca acttgcagca gtgcgagggt cgaaccttca agactcacca tgaaacgctt
     2461 gactacatca aaacgctggg gttcccggta tcaccgcggt acagcgtatt tgagaatatc
     2521 gaggacgcca tcaaagaaat tgaatccatc ggcgaagccc gcggcacgct ggaatttgac
     2581 attgacggcg cagtcatcaa ggtcaacgac ctggccgccc gccgcacgct gggcagcacg
     2641 aacaagttcc cgcgctgggc gattgcattc aagtatccgc ctgaggttaa agagagcgtc
     2701 gtccgtgata ttgaagttac cgtcggccgc accggcgtac tgacgccgac ggcggtgttt
     2761 gacccgattt tcttggcggg caccagcgtt tcccgcgcca gtctgcacaa cggcgatatc
     2821 attgcaaacc tcggcgttgg cattggcgat accatcaagg tgcgcaaagc gggggacatc
     2881 atccccgagg tcattggcgt ttcggcccgg ctgccgggca gcaagacttt tacgatgccc
     2941 acggtctgcc ccagctgtgg cgcaccggtc gtgcatttgc aggacgaaac ggccctgcgc
     3001 tgcgtcaacc ccgagtgccc ggcccaaagc ctgcgcaacc tcatccactt tgcgtcccgc
     3061 aacgccatgg ccattgacgg cctgggcgag gctgtggccg tgcagctcat cgacaaaggg
     3121 ctggtcagca ccgtggccga tctgtacacc ttaactgagg aacagctgct taccttagac
     3181 aagttcaaga aaaagagcgt gcagaatttg ctgaatgcca ttgagggttc caagcgcaac
     3241 aacctcgaca agttgatttt cggcctgggc atccgcaaca tcggcgacaa ggccgccgca
     3301 ctgctgggcg agcatttcgg cagcatggac gcacttcgcc aggccacggc cgaacagatg
     3361 tgcgagatcg acggctttgg cgaggtcatg gcccagagcg tgctggaatt ttttgcaaag
     3421 gacggaacga ccgacctgct gcgccgcctg gccgacgacg gcgtgaatat gcagtggacc
     3481 ggcgaaaaga agggcacagc actggccggt atgacgctgg tcgtgaccgg cacgttgccg
     3541 acgctctccc gtcaggaagc cgaagccctc atcacccaga acggcggcaa ggcggcaggg
     3601 tctgtcagca aaaagacgag ctacgttgtg gccggtgagg ccgccggttc caaattaacc
     3661 aaagcccaga cactgggcat tccggtattg gacgaggccg gactgtatag gctgattgag
     3721 gaaaacgacc aataacgcgc ggacgcatcg catatagaat gcacccctgc aaccgtaagt
     3781 gtcggcatcc tcgatgaccc acaaaacatc acacttttct actggacatt tccattcggg
     3841 gatgtcctct tttttgttct atccgcccgc acgaaacgga aattctgaac atcggtatca
     3901 gttatttac
//
LOCUS       DBMDSQ010000195         3888 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_5410_length_3888_cov_1.612886, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000195 DBMDSQ010000000
VERSION     DBMDSQ010000195.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 3888)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3888)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3888
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5410_length_3888_cov_1.612886"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..986)
                     /locus_tag="ACZPAX_07385"
     CDS             complement(<1..986)
                     /locus_tag="ACZPAX_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1113760.1"
                     /translation="MTKDLTSGSPLKVILMFTFPLVLGNLFQQFYALADTIIVGRFCG
                     VSALASVGATGSVNYLILGFVIGVCNGFAIPIAQLFGARDYKDLRRHVANAAWLCIAA
                     SVVLTIATVALTRPMMQLMQTPDDIIDGAVIYIGWIFAGIPFIFLYNMVAAIMRALGD
                     SKTPLYFLILTSALNIGLDLLFVIPFKFGVFGAALATDISQVISGVLSFIYLCRKFDV
                     LKMQKDDLVFSKKACVRLMGIGVPMGLQCSITAIGSVIMQWAVNMLGSTAVAAVTAAG
                     KTQNLLTVPLESVGTAMATYAGQNLGASRMDRVRKGTNSAMLIILVYGLASA"
     gene            complement(1144..2832)
                     /locus_tag="ACZPAX_07390"
     CDS             complement(1144..2832)
                     /locus_tag="ACZPAX_07390"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF024703.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HIR1113761.1"
                     /translation="MLFPALKAVGEGGPIFYLTARGTTRAAAENALTLLRAADPDLKL
                     RSVTLTAKDKICLQEHRECTPEACPYANGYYDRVKAALWDGLDTRALTADALQALARK
                     HKVCPFELGLDLSLWCDVVVGDYNYLFDPVVHLMRFFETAGDYLFLIDEAHNLPGRAR
                     DMHSASLCKSVFYDAKKRLGKGKSSLKNTLTKVNNIFIEWRHRCEEVLGDSRFGRTYF
                     EKSRAEDFDRALTKLCEPLEIWLDEHRDPSETHDALLQLYFDIRAWLRVADTFDNHFV
                     LQISAVGSEVRAAMLCLDPSDFLAADFAKGRAAVLFSATLAPAGYYKDLCGLPDARAV
                     ALRSPFDPTNMGLWCARQVSTRYKDRADSIAKVSDLLAVMAAAQPGHYLAFFPSYSYL
                     QQVWEDFTARYPDQPTLCQESAMDEGQRTEFLAQFLARDGKPLLGFAVLGGVFGEGVD
                     LTGESLIGVAVVSPGLPQIGPRQEQLRDYFEETRGAGFDYAYRYPGMNKVLQAAGRVI
                     RTPQDRGVVLLIDDRFLAPDTRRLMPPHWEHLRVVDSADAWKDELAAFWKKHEP"
     gene            complement(2863..3528)
                     /locus_tag="ACZPAX_07395"
     CDS             complement(2863..3528)
                     /locus_tag="ACZPAX_07395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113762.1"
                     /translation="MEPEILALTLPIRRLVEFLLRTGSIDSRFTGFDRALEGARLHRK
                     LQRAAVKKYPDYQAEAALKQDYTCAGIAYTLEGRADGIFTDKDGTPTIDEIKTTTLPP
                     EFITGEQSPEHWAQAQIYAAIYARQQGLPAMRVRLVYYQVDEDLEFTFNHDYSADALD
                     AIVTDLLTQYAPWAKRSAEWQRMSRASRQALPFPFASYRPGQRAMMNAVYKTCTEGGQ
                     LLC"
     gene            complement(3565..>3888)
                     /locus_tag="ACZPAX_07400"
     CDS             complement(3565..>3888)
                     /locus_tag="ACZPAX_07400"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HIR1113763.1"
                     /translation="IVKWRPPQNRDPLPEESTACMPWLRQQFQLIRPKIVVCLGRIAA
                     QQMMGPTFSVSRDHGKFFEKQGTLFMATYHPAALLRYPVNKPAVFGDFVALREKITQV
                     CDHTY"
BASE COUNT          800 a         1145 c         1177 g          766 t
ORIGIN      
        1 aacgcggatg ccaagccata caccagaatg atcagcatag cgctgttggt acccttgcgc
       61 acacggtcca tgcgggatgc gccaaggttc tgcccggcgt aggtcgccat agccgtgccg
      121 acgctttcca gcgggacggt cagcaggttc tgcgttttgc cggcagcggt gacggcggcc
      181 acggcggtac tgcccagcat attgacagcc cactgcatga taacgctgcc gattgccgtg
      241 atgctgcact gcagacccat gggaacgcca atgcccatca gccgcacaca ggcttttttg
      301 ctgaacacaa ggtcgtcctt ctgcatcttg aggacatcaa acttgcggca gaggtagata
      361 aagctcagca cgccggaaat gacctgggaa atgtcggtcg ccagcgccgc gccgaacaca
      421 ccgaacttga acggaatgac gaacagcaag tccagtccga tgttcagcgc actggtcaaa
      481 atcaggaagt aaagcggcgt tttgctgtcg cccaaggcac gcatgatagc cgccaccatg
      541 ttgtacagaa agatgaatgg gatgcccgcg aaaatccagc caatatagat gacggcaccg
      601 tcgataatat cgtcgggggt ctgcatcagc tgcatcatgg gacgggtcag cgccaccgtg
      661 gcaatggtca gcaccacgct ggccgcaatg cagagccagg cggcgttggc cacatggcgg
      721 cgcaggtctt tatagtcccg cgcaccgaac agctgcgcaa tggggatggc aaagccgttg
      781 catacgccga tgacaaagcc cagaatcaga tagttgaccg agccggtcgc accgaccgat
      841 gccagcgcac tgacgccgca gaaccgacca acgatgatgg tatccgccag tgcgtagaac
      901 tgctggaaca ggttgcccag caccagcggg aacgtgaaca taagaataac tttgagcgga
      961 ctgccgctcg tgaggtcctt ggtcatggat ttttccttac ccttatatgt atacaaaatt
     1021 tcgtgcgaat cgtattatag cacgtacata tataaatgca agcggataat ccatgcaaat
     1081 tttgcgcact ttttcgccat ccgcacaaaa aggaagaaac cgcccgctga cggttgtttt
     1141 cgcttatggt tcgtgctttt tccagaaggc cgccagttcg tctttccacg cgtcggcaga
     1201 atctaccacc cgcagatgct cccagtgggg cggcatcagg cggcgagtgt caggggccag
     1261 gaatcggtcg tcaatgagca ataccacgcc cctgtcctgc ggcgtgcgga tgacccgccc
     1321 cgccgcctgc aagaccttgt tcatgccggg gtagcggtag gcatagtcaa aacccgcgcc
     1381 gcgggtctcc tcaaagtaat ctcgcagttg ttcctggcgg ggcccgatct gcggcagacc
     1441 ggggctgaca acggctacac cgatcaagct ctcccctgtc aggtctacgc cctcgccaaa
     1501 gacgccgccc agtacggcaa acccgagcaa gggctttccg tcccgcgcca agaactgcgc
     1561 cagaaactcc gtgcgctggc cctcgtccat ggcggattcc tggcagaggg tgggctggtc
     1621 cgggtagcgg gcggtgaaat cctcccacac ctgttgcaag tagctgtaac tggggaagaa
     1681 ggccaggtag tgcccaggct gtgcagcggc catcacggcc agcagatccg acactttggc
     1741 aatgctgtct gcccggtcct tgtagcgggt gctgacctgc cgggcgcacc acagccccat
     1801 attggtcggg tcaaaagggc tgcgcaatgc cacggcgcgg gcgtcgggca gaccgcacaa
     1861 atccttgtag taacctgccg gggccagtgt ggcgctgaac agcaccgccg cacggccctt
     1921 ggcaaagtct gccgccagga agtcactggg gtccaggcag agcatagcgg cgcgaacctc
     1981 gctgccgacg gcggatatct gcaaaacaaa gtggttgtca aacgtgtctg ccacccgcag
     2041 ccaggcgcgg atatcaaagt acagctgcaa cagtgcgtcg tgggtctcgc tggggtcgcg
     2101 gtgttcgtcc agccaaattt ccagcggctc acagagtttt gtcagcgcac ggtcaaagtc
     2161 ctcagcacgg gacttttcaa agtaggtgcg gccaaagcgg ctgtcgccga ggacttcttc
     2221 gcagcggtgc cgccattcaa taaaaatatt gttgaccttt gtcagcgtat ttttcaggct
     2281 gcttttgcct tttcccagcc gcttttttgc gtcatagaag acgcttttgc aaaggctggc
     2341 gctgtgcata tcccgggcgc ggccgggcag attgtgtgct tcatcgataa gaaacaggta
     2401 gtcccccgct gtctcaaaaa aacgcatcag gtgtacgacc gggtcaaaga ggtagttgta
     2461 gtcccctacc actacgtcac accacaggct caaatcaagc cccagctcaa aggggcagac
     2521 tttatgcttg cgggccagcg cttgcagggc gtcggcggtc agggcgcggg tatcgaggcc
     2581 gtcccacagc gccgccttga cgcggtcata gtagccgttg gcgtaggggc aggcctcagg
     2641 tgtgcattcg cggtgctcct gcaagcagat tttgtctttg gcagtcagag tcacgctgcg
     2701 cagcttcaaa tcagggtctg ctgcgcgcag cagagtcagt gcattttctg ctgccgcgcg
     2761 ggtcgtgccc cgggccgtca ggtaaaaaat cggcccgcct tcccccacag ctttcagtgc
     2821 cgggaacagc acactcatcg tcttgccgat accggtggga gcctagcaga gcagctggcc
     2881 gccctcggtg caggtcttgt acacggcgtt catcatagcc cgctgaccgg ggcggtagct
     2941 tgcaaacgga aatggcagtg cctgccgcga tgcacggctc atccgctgcc actcggccga
     3001 gcgcttggcc cagggcgcgt actgcgtcag caggtcggtg acgatggcgt ccagcgcatc
     3061 ggcggagtag tcgtgattga aggtaaattc cagatcctca tccacctgat agtaaaccag
     3121 ccgcacccgc atggcgggca gcccctgctg gcgagcgtag atggccgcgt agatctgcgc
     3181 ctgcgcccag tgctcggggc tttgctcgcc ggtgatgaat tcaggcggca gcgtcgtggt
     3241 cttgatctca tcaatggtcg gtgtgccgtc cttgtcggta aagatgccat cggcgcggcc
     3301 ttccagcgtg taggcaatgc cggcgcaggt gtagtcctgc tttagggcag cttcggcctg
     3361 gtagtcgggg tatttcttca cggcggcgcg ctgcaatttg cggtgcaggc gtgcgccttc
     3421 cagcgcacgg tcaaagccgg taaaacggct gtcgatgctg ccggtgcgca ataaaaattc
     3481 caccagacgc cggatgggca gcgtcagtgc caaaatttca ggctccatgc cgcgtgcctc
     3541 cttgcatcgg gtggtatact aaagttaata ggtgtggtcg cagacctgcg tgattttctc
     3601 acgcagggcc acaaagtcgc cgaacacggc aggcttgttt acgggatagc gcagcagcgc
     3661 cgcagggtgg taggttgcca tgaacagtgt gccctgcttc tcaaagaatt tgccgtgatc
     3721 gcggctgacc gagaaggtcg gccccatcat ctgctgcgcg gcgatgcggc ccagacagac
     3781 aacaatcttg ggtcgaatca gctggaactg ctggcgcagc cacggcatac aggcggtgga
     3841 ttcctccggc agcgggtcgc ggttctgggg cgggcgccat ttgacgat
//
LOCUS       DBMDSQ010000196         3875 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_5431_length_3875_cov_2.309486, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000196 DBMDSQ010000000
VERSION     DBMDSQ010000196.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 3875)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3875)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3875
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5431_length_3875_cov_2.309486"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1164)
                     /locus_tag="ACZPAX_07405"
     CDS             complement(<1..1164)
                     /locus_tag="ACZPAX_07405"
                     /inference="COORDINATES: protein motif:HMM:NF027890.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HIR1113764.1"
                     /translation="MAEQTKTRRGPLAWVKRHKKLTIFLVIVLVIALVITNVVRKANK
                     AASGTTYQYVRTTVLQKTTLSDSVNVNGTVKSGSSASVTASDSVKTYKVTAVNVAVGD
                     TVKKGDVIATLDTTDVEKQIANAELKLSDTQTDAQKSYDQAVEDQSTDLASARADLEE
                     AQKKYDTLGLDDYYGSMADGSNSGKTNREIVTEYYTRYAEEIAGYENTLANLRIQLTQ
                     AQQDGDADRAAGLQGQIDDYTNRESVAKGQCSIPELGLQGFDAVSQCYNQIEQLAQAL
                     DKAQQSYQKAATSASRSVDNAETKLEQSQREDDTLTSLQTALDNCTLTATMDGTITAL
                     NATVGDVCSGAVATIQDVDNLTVEVTIPASSVGKLKAGMQCNITSDATEDTVTG"
     gene            complement(1477..2469)
                     /locus_tag="ACZPAX_07410"
     CDS             complement(1477..2469)
                     /locus_tag="ACZPAX_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009003951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1113765.1"
                     /translation="MHSSLIAWILRAIPFGTIIMYGALGETVTEKSGNLNLGVPGIMY
                     LGGFAGFASAWAYENTAANPSGAVSVVIALTCAILASMAGGLIYAFLTISLRANQNVT
                     GLALTTFGMGVANFFGVFILNGASYSAAPIANKAFSAKIPVLSTLGGFGQVVFSYGFL
                     VYAAIALAIALHWFLTRTRAGLNLRAVGENPATADAAGINVTLYKYLATCIGAGICGI
                     GGLYYVLDYNQGIWATTGQIEALGWLAVALVIFTTWKPLNAIWGAYLFGMLYWLYQFL
                     PTLLGITMASHITDLVQMLPYVVTIVVLIVVSMRKKKENQPPAHLGLAYFREER"
     gene            complement(2471..3574)
                     /locus_tag="ACZPAX_07415"
     CDS             complement(2471..3574)
                     /locus_tag="ACZPAX_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014118678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1113766.1"
                     /translation="MSQTAAAAREPLLRIAKREGTTMPQKIAVRAIAILLALVVDALF
                     IFFVTGLNPLAVYGVMWGGTFANMTRFSWMLRDLSTLLCVGIALAPAFKMRFWNCGGE
                     GQILIGGLTAALIMVYQGNNLPLPLLFAAMALGSIAAGALWGFIPAWFKSRWNTNETL
                     FTLMMNYVATSIVACMTNIMRGQASSLGTLNKATKAGWFPVILGQRYTINIIVVIVLT
                     FVMYAYLRFSKQGYEISVVGESENTARYAGINVRRVTVRTMLISGAICGLCGFLIVAG
                     KDQTISTASANGRGFTAIIVAWLAKFNTFYMALISFLLVFLERGASEIASAYSLNEYA
                     ADIITGIILFFILGSEFFINYRVIPRGAHKEGK"
     gene            complement(3571..>3875)
                     /locus_tag="ACZPAX_07420"
     CDS             complement(3571..>3875)
                     /locus_tag="ACZPAX_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016322304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1113767.1"
                     /translation="VQKVLVGRELAADPIVLMTAYAVRGLDINTSYTIYHLLNEQKKR
                     GVAVIYVGEDLDVLLELCDRIVVLCGGKVNGILDGRKTTKEEVGLRMTNLVKEEQA"
BASE COUNT          749 a         1144 c         1208 g          774 t
ORIGIN      
        1 gccggtgacg gtgtcctcgg tagcatcgct ggtaatgttg cactgcatgc cggctttcag
       61 ctttcccacc gagctggcgg ggatcgtgac ctcgacggtc aggttgtcca cgtcctgaat
      121 ggtggcaact gcaccgctgc acacgtcacc cacggtagcg ttcagcgccg tgatggtgcc
      181 gtccatcgtg gcggtcaggg tgcagttatc cagtgcggtc tgcaagctgg tcagggtgtc
      241 gtcctcgcgc tgggactgct caagcttcgt ctcggcgttg tccacgctgc gggacgcgct
      301 ggtggcagcc ttctggtagg actgctgggc cttgtccagc gcctgggcca gctgctcgat
      361 ctggttgtag cactggctga cggcgtcaaa gccctgcagg cccagctccg ggatgctgca
      421 ctggcccttg gcgacgctct cacggttggt gtaatcgtcg atctgcccct gcagacccgc
      481 ggcgcggtcg gcatcgccgt cctgctgggc ctgggtcagc tggatgcgca ggttcgccag
      541 ggtgttttcg tacccggcaa tttcctcagc gtagcgggtg taatactcgg tgacgatctc
      601 acggttcgtc ttgccgctgt tgctgccgtc ggccattgag ccgtagtagt catccaggcc
      661 gagggtgtcg tactttttct gggcttcttc cagatccgcc cgggcactgg ccaggtcggt
      721 ggactgatcc tcgacagcct ggtcatagct cttctgagcg tcggtctggg tgtcgctgag
      781 cttcagctcg gcgttggcga tctgcttttc cacatcggtg gtgtccagcg tggcaatgac
      841 gtcgcccttt ttgaccgtat cgcccacagc cacattgacc gccgtgacct tgtaggtctt
      901 gacggaatcc gaggcggtga cgctggcgct gctgccggac tttaccgtgc cgttgacgtt
      961 gaccgaatcg ctgagggtcg tcttttgcag gacggtcgtg cgcacatact ggtaggtcgt
     1021 accggaggcc gccttattgg ccttgcgcac aacgttggtg atgaccagcg cgataacgag
     1081 cacaatgacc aaaaagatcg tcagtttttt gtggcgtttg acccaagcca acggaccgcg
     1141 ccgggttttt gtttgttctg ccataccggg ttcccttctg tgtgtggaga ttttttgaca
     1201 aaatttagta tagggggtaa atgtgtttaa actatgaaag aagcatgaaa aagcggcaca
     1261 ccttttggaa agtgtgccgc tgtataagtc gtgctatggt aggggtcgat gcccgtatcg
     1321 acctgcgggg cgtcgaggac gccgcctcct acaaaacctg ttagtgccgt aggggccggg
     1381 catgcccggc ccgcaacata tggaataccg cgcagttcgg gccaagctga gggccggaca
     1441 tgtccggccc ctaccccgct cctttttcgc ccgggtttac cgctcctcgc ggaaataggc
     1501 caggcccagg tgtgccgggg gttggttttc tttcttcttg cgcatggata ccacaatcaa
     1561 aaccacaatc gtaacgacgt agggcagcat ctgcaccagg tcggtgatgt ggcttgccat
     1621 cgtgatgccc agcagcgttg gcaggaactg atacagccag tagagcatac cgaacaggta
     1681 cgcaccccag atggcgttca gcggtttcca ggtcgtgaag atgaccagcg ccacggccag
     1741 ccagcccagc gcctcgatct gcccggtggt ggcccagatg ccctggttgt agtccagcac
     1801 gtagtacagg ccgccaatgc cgcagatgcc cgcgccgata caggtcgcca ggtacttgta
     1861 cagcgtaacg ttgatgcccg ccgcatctgc ggtggcgggg ttctcaccca cggcgcgcag
     1921 gttaaggccc gcgcgggtgc gggtcaaaaa ccagtgcagc gcaatcgcca gtgcaatggc
     1981 ggcatagacg aggaacccat agctgaacac gacctgcccg aagccgccca acgtggacag
     2041 caccgggatt ttggcgctga atgccttgtt ggcgatgggg gcagcagagt agctggcgcc
     2101 gtttaagata aacacgccga agaagttcgc cacgcccata ccgaaggtcg tcagcgccag
     2161 gccggtgacg ttctggttgg cgcgcaggga aatcgtcagg aatgcgtaaa tcaggccgcc
     2221 cgccatggat gccagaatag cgcaggtcag ggcaatgaca acgctgaccg cgccgctcgg
     2281 gttggcggcg gtattctcat aggcccaggc gctggcaaag ccggcaaagc cgcccaggta
     2341 catgatgccc ggcacgccca ggttcaggtt gccggatttc tcggtcacag tctcgcccag
     2401 cgcaccgtac atgataattg taccaaaggg gatggcgcgt aaaatccatg caatcaaact
     2461 gctgtgcata tcacttgccc tccttgtgtg cgccgcgcgg gatgacgcgg tagttgatga
     2521 agaactcact gccgaggatg aagaagagga tgatgccggt gatgatgtca gcagcgtact
     2581 cgttcagcga gtaggcggac gcaatctccg acgcgccgcg ctccaggaac acgagcagga
     2641 aggagatcag cgccatatag aaggtgttga actttgccag ccaggccacg atgatagccg
     2701 tgaagccgcg cccattggcg gaggccgtgg aaatcgtctg atccttgccc gcgacgatga
     2761 ggaagccgca caggccgcag atggcaccgg aaatcagcat cgtgcgcacc gtgacgcggc
     2821 ggacgttgat gcccgcgtac cgcgcggtgt tctcgctctc accgacgacg gaaatttcat
     2881 acccctgctt ggagaagcgc aggtaggcgt acatgacgaa ggtcagcaca atgacaacga
     2941 tgatgttgat cgtatagcgc tggcccaaaa tgaccgggaa ccagcccgcc ttggtggcct
     3001 tgttcagcgt gccaaggctg gacgcctggc cgcgcatgat gttggtcatg caggcgacga
     3061 tcgacgttgc cacgtagttc atcatcaggg taaacaacgt ctcgttcgtg ttccagcggg
     3121 acttgaacca cgcagggatg aagccccaca gcgcaccggc ggcaatgctg cccagcgcca
     3181 tggccgcaaa cagcagcggc agcggcaggt tgttgccctg gtacaccatg atgagggcgg
     3241 cggtcaggcc gccaatcaaa atctgaccct cgccgccgca gttccagaag cgcatcttga
     3301 acgcgggggc cagcgcgatg ccgacgcaga gcagcgtgga cagatcgcgc agcatccagc
     3361 tgaagcgggt catgttggca aaggtgccgc cccacatgac gccgtagacg gccagcgggt
     3421 tcaggccggt gacgaaaaag ataaacagcg catcgacgac cagcgccagc agaatggcaa
     3481 tggcacgcac agcgatcttc tggggcatcg tggtcccctc gcgcttggca atgcgcagca
     3541 gcggctcgcg ggccgcagcg gcagtctgac tcatgcctgc tcctccttta ccaggttggt
     3601 catacgcaga ccgacctctt ccttcgtggt cttgcggccg tcgagaatgc cgttgacctt
     3661 gccgccgcag agcacgacga tacggtcaca cagttccagc aggacgtcca gatcctcgcc
     3721 gacataaatg accgccacac cgcgcttttt ctgctcgttc agcagatggt aaatcgtgta
     3781 ggacgtgttg atatccagcc cgcgcaccgc gtaggcggtc atcaatacga tggggtcggc
     3841 ggccagttcg cggcccacca gcaccttctg cacgt
//
LOCUS       DBMDSQ010000197         3860 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_5461_length_3860_cov_1.830540, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000197 DBMDSQ010000000
VERSION     DBMDSQ010000197.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 3860)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3860)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3860
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5461_length_3860_cov_1.830540"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..351)
                     /locus_tag="ACZPAX_07425"
     CDS             complement(<1..351)
                     /locus_tag="ACZPAX_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylyltransferase domain-containing protein"
                     /protein_id="HIR1113768.1"
                     /translation="MKTIAVIPARYASTRMPGKPLADVLGKPMIYWVYQAAKACPKLD
                     DVLIATDDERIADACKAYDMRYLMTSPDHDTPTGRIWEVSTVENADLYLQLMGDEPLV
                     NPAAFDLILPSTLPE"
     gene            complement(498..1472)
                     /locus_tag="ACZPAX_07430"
     CDS             complement(498..1472)
                     /locus_tag="ACZPAX_07430"
                     /inference="COORDINATES: protein motif:HMM:NF019535.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 52"
                     /protein_id="HIR1113769.1"
                     /translation="MKPNLYICHTAYQVLVDLLRAGRCACENGPHTMVLSASVPDTAV
                     LAARLDAAGVVKTVIVDETKWPGTVTGPFAHQRAARAFEKLCGWKFDRAAYENVYIHN
                     DWSVLGRYMQDCRAGYILCEDTFGSTLGPDQHLVTDQRAAADFAAKQRGKGYLYWGDS
                     PWCVCIESEDAAKCTLFPAARMVTDSKAILLESLTDAEKALVCRIFLTQPLPEKADGA
                     TLLLPRSFVADGLMTQGQQDAMFKAVAEKYAVGPLFIKTHPRDTTDYKALFPDATVLE
                     RTMPSEVLNFCLPFHFARAVTVQSFVLRGFTAADEKIHLTLDEARALV"
     gene            complement(1476..2753)
                     /locus_tag="ACZPAX_07435"
     CDS             complement(1476..2753)
                     /locus_tag="ACZPAX_07435"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1113770.1"
                     /translation="MSATEKRPVIEVSGLKKQYKLGQIGGGTLTHDLQSWWARVRGKE
                     DPNTVIGTDQRLFGQTFMALNGVDLTVYQGEALGIIGRNGAGKSTLLKLLSRITAPTE
                     GEIKIRGRVASMLEVGTGFNNEMTGRENIYMNGAILGMTRAEIDAKLPQIIEFSECGD
                     FIDTPVKRYSSGMFVKLAFAVAAHLDSEIMVMDEVLAVGDMKFQQKCLGKMSDVANQD
                     GRTVLYVSHNMSTIRQLCTRCVVLDKGKVIFDGDVEQAIAVYMDTTDVNVVHYDLADV
                     ARMTGSAGKRFRLETLDFVGKESSVFADTEKMRVKITWRVSEPFAGIHMKMNLHARDS
                     TPVGITHPVDLGSAEPGKVYTSVFEFDPSLLGEGQYFFYLDIFDGALAHAVCLDKPVT
                     EFAFEVTNSDLTMPEWASGWGRIHFPPVKLVQE"
     gene            complement(2772..3650)
                     /locus_tag="ACZPAX_07440"
     CDS             complement(2772..3650)
                     /locus_tag="ACZPAX_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1113771.1"
                     /translation="MAENLQTTRPAEEGWTTVIRPKTGWFDIDLNELWHYRDLITMFV
                     KRNFTVLYKQTILGPAWIILNPLITTIIFNVVFGGMANMPTDGVPGFLFYMAGNTVWT
                     FFANCVNNTANTFVTNSQVFGKVYFPRLTMPISQVLTSLINFGIQAVMYLIFWGYFFA
                     TGSGITFTLWALAIPFVMLEVMLLGLGVGIIVSSLTTKYRDLAIAVGFGVQLWMYASP
                     VVYPLSMLDNSPRLKVLVQLNPMTSPIEIFRMATLGTGTVTVFGIVYSLIFTAAALVL
                     GVVLFSRIEKTFMDTV"
BASE COUNT          808 a         1126 c         1124 g          802 t
ORIGIN      
        1 ctcgggcagg gtgctgggca ggatcaggtc aaacgcggcc gggttgacca gcggttcgtc
       61 gcccatcagc tgcaagtaga ggtctgcatt ctccacggtg ctgacctccc agatgcgccc
      121 ggtgggggta tcgtggtcag ggctggtcat caagtagcgc atatcgtagg ctttgcaggc
      181 gtcggcaatg cgctcgtcgt cggtggcaat aagcacatcg tccagcttcg ggcaggcctt
      241 ggcggcctgg tagacccagt agatcatcgg tttgcccagc acatccgcca gcggcttgcc
      301 cggcatccgg gtactggcgt aacgcgcggg gataacggcg attgttttca tggtattata
      361 cctcgcatat ttggcttata tataaaatga accacgcatg taggggccgg gcatgcccgg
      421 cccacagctt tacggaaagc gctctattac gatcaggttg agggcgggac atgtcccgcc
      481 cctacagttg caacccttca caccagcgcc ctcgcctcgt ccagcgtcag gtggattttt
      541 tcatccgccg cggtgaaccc gcgcaaaaca aagctctgca cggtcacggc gcgggcaaaa
      601 tggaagggca gacagaagtt cagcacctcg ctgggcatcg tgcgctccaa cacggtagcg
      661 tcagggaaca gggctttata gtccgtcgtg tcccgggggt gggtcttgat aaacagcggc
      721 ccaacggcat atttttccgc taccgccttg aacatcgcgt cctgctgtcc ttgggtcatc
      781 agtccgtccg ccacaaagct gcggggcagc aaaagcgttg cgccgtcggc cttttcgggc
      841 aggggctggg tcaaaaatat gcggcacacc agcgcttttt cggcatcggt caggctctcc
      901 aacagtatgg ccttgctgtc cgtcaccatg cgcgcagcgg ggaacagcgt acacttcgcg
      961 gcgtcctcgc tctcgatgca cacacaccac gggctgtcgc cccagtagag gtagcctttt
     1021 ccgcgctgtt tcgcggcaaa atcggccgcc gcgcgctggt cggtcacaag atgctggtcg
     1081 gggccaaggg tgctgccgaa cgtgtcttca cacaagatat acccggcgcg gcagtcctgc
     1141 atatagcggc ccagcacgct ccaatcgttg tggatataca cgttttcata cgctgcgcgg
     1201 tcaaatttcc agccgcacag cttctcaaac gcccgcgccg cacgctggtg ggcaaagggg
     1261 cctgtcactg tgccgggcca ctttgtctcg tccacaatga ctgtctttac aacgccggcc
     1321 gcgtccagcc gggccgccag cacggcggtg tcgggcacgg acgcggaaag caccatcgtg
     1381 tggggcccat tttcacaggc gcagcgcccg gcccgcagca ggtctaccaa aacctgatag
     1441 gccgtgtggc agatgtacaa attcggtttc atggcttact cctgcaccag cttgaccggc
     1501 gggaagtgga tgcgccccca gccggacgcc cactcgggca tcgtcaggtc gctgtttgtc
     1561 acttcaaagg cgaactcggt cacaggcttg tccaggcaaa ccgcatgggc cagtgcgccg
     1621 tcaaaaatat caaggtagaa gaaatactgt ccctcgccca gcaggctcgg gtcaaactca
     1681 aacaccgagg tgtagacctt gcccggctcg gcagagccga ggtccaccgg gtgggtgatg
     1741 cccaccggcg tcgagtcccg cgcgtggagg ttcatcttca tgtggatgcc tgcgaacggc
     1801 tccgacacgc gccaggtgat tttcacgcgc atcttctcgg tgtcggcaaa gacgctcgat
     1861 tctttcccga cgaagtccag cgtttccagc cggaaccgct tgccggcact gccggtcatg
     1921 cgggccacat cggccaaatc gtagtggacg acgttgacgt cggtggtgtc catgtagacc
     1981 gcgatagcct gctcgacgtc gccgtcaaag atgaccttgc ccttgtccag cacgacacag
     2041 cgggtgcaaa gctgacggat cgttgacata ttatggctga catacagcac cgtgcggccg
     2101 tcctggttgg caacgtcgct cattttccct aagcatttct gctggaattt catgtcgccg
     2161 acggccagca cctcgtccat gaccataatt tcactgtcga ggtgcgcggc tacggcaaag
     2221 gcaagtttca cgaacatacc gctggagtag cgcttgacgg gggtgtcaat aaaatcgccg
     2281 cactcggaaa attcgatgat ctgcggcagt tttgcatcga tttcggcgcg ggtcatgccg
     2341 agaatcgcgc cgttcatata gatgttctca cggcctgtca tttcgttgtt gaagccggtg
     2401 ccgacctcca gcatcgacgc cacgcggccg cggattttga tttcgccctc ggtgggggcg
     2461 gtgatgcggg agagcagctt taacagcgta gatttgcccg cgccgtttcg gccgatgatg
     2521 cccagcgcct cgccctggta gacggtcagg tcaacaccgt tcagtgccat aaaggtttgc
     2581 ccaaacagcc gctggtcggt gccgatgacg gtgttcgggt cctccttccc gcgcacgcgg
     2641 gcccaccagc tttgcaggtc atgcgtcagc gtgccgccgc cgatctggcc gagcttgtac
     2701 tgttttttca gccccgaaac ttcgatgacc gggcgttttt ctgttgcaga cataattcaa
     2761 gcgccccttt cttacaccgt atccataaac gttttttcaa tgcggctgaa cagcacaacg
     2821 cccaaaacca gcgccgcggc ggtaaaaatc agactgtaca cgatgccaaa caccgtgacc
     2881 gtgccggtgc ctaaggtcgc catgcggaaa atctcgatgg gcgacgtcat ggggtttagc
     2941 tggaccagca ctttcagacg cggggaattg tccagcatcg acagcgggta gacgaccggg
     3001 gaagcgtaca tccaaagctg cacgccgaag cccacggcga tggctaaatc gcgatatttc
     3061 gtcgttaaag aggaaacgat gatgccgacg ccgaggccca gcagcatgac ttccagcatg
     3121 acgaagggaa tcgccagcgc ccacagcgta aaggtgatgc cgctgcccgt ggcgaagaaa
     3181 tagccccaga aaatcaggta cataaccgcc tggatgccaa agttgataag gcttgtcagc
     3241 acctggctga tgggcatggt caggcggggg aagtagacct tgccgaacac ctgcgagttt
     3301 gttacaaacg tattggccgt gttgttgacg cagttggcga aaaacgtcca gaccgtgttg
     3361 cccgccatgt agaacaaaaa gccgggtacg ccgtctgtgg gcatattggc catgccgccg
     3421 aacacaacgt tgaagatgat agtggtgata agcggattca gaatgatcca cgccgggcct
     3481 agaatcgtct gtttgtacag cacggtaaag ttgcgcttga caaacatcgt gatgaggtcg
     3541 cggtagtgcc acagctcgtt caaatcaata tcgaaccagc ctgttttggg ccgtataacg
     3601 gtggtccagc cctcctcggc cgggcgggtg gtttgcagat tttcagccat aaaaatacct
     3661 gttccttatg tgtaaagtcg tagtcgcaca attatccata atagtagtgc tattttacca
     3721 cattatgcgc aaagaatcaa cttgccaaag cgtttataat aatagtagag gccgggcata
     3781 cccggcccgg tcgtgaaagg catgggcgtt tcccggaagg cggtgggccg cataggtgcg
     3841 gcccctacaa ctcaacataa
//
LOCUS       DBMDSQ010000198         3848 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_5480_length_3848_cov_2.041633, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000198 DBMDSQ010000000
VERSION     DBMDSQ010000198.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 3848)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3848)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3848
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5480_length_3848_cov_2.041633"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..452
                     /locus_tag="ACZPAX_07445"
     CDS             <1..452
                     /locus_tag="ACZPAX_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005807020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HIR1113772.1"
                     /translation="RWGIKVNRVELKNIEPPVEIRQAMEKQMKADREKRASILLAEGE
                     KQAAITRAEGEKESAILRAEAVKQQRIREAEGEAEALLTVQKAQADAIRMINEANPNH
                     NYLALRSMEAMEKVADGKATKLIVPSDMQNLAATVSAIKEISGEVTE"
     gene            653..1261
                     /locus_tag="ACZPAX_07450"
     CDS             653..1261
                     /locus_tag="ACZPAX_07450"
                     /EC_number="2.7.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007045559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0050145 - nucleoside monophosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HIR1113773.1"
                     /translation="MKEPMVITMAREYASGGSEIAQAVADKLGIPLYNKELITLAAKK
                     SGLTEEAIAASENQRSGSLIYSLYMMGNTMPLADQVYILQSNVIKELAAQGSCVILGR
                     CGDYVLRERPNVLRTFVYAPLDARIGFAKSRPDAKDMPDRLWETQLAKHDRARASYYN
                     YYTENRWGEAKNYDLCLNATLGREACADLIVAAAKAMQAANK"
     gene            1284..1961
                     /locus_tag="ACZPAX_07455"
     CDS             1284..1961
                     /locus_tag="ACZPAX_07455"
                     /inference="COORDINATES: protein motif:HMM:NF015674.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009268 - response to pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HIR1113774.1"
                     /translation="MLRYVKSGMMLLSIAYIVIGMMLLIMPQTSLLWICYAFGAVVLI
                     TGIVCLIQYARIRGTGFTAPFMLVGGVITAGLGIFTLAKPQVVASFLPIVFGIFIVVD
                     GLSRIGSAIDLAKRKGQKWWVLLLLSIVSVALGILLVLHPFGAAVSVVMVCGILLIVE
                     GAMNLGCILYAAMELRTLDRMATAAANAAMSAIGDALDEAEAAESPADTPVVYDAESK
                     DVSEDGE"
     gene            2130..3533
                     /locus_tag="ACZPAX_07460"
     CDS             2130..3533
                     /locus_tag="ACZPAX_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007045557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR1113775.1"
                     /translation="MYDLIIVGAGPAGIFTALELLRKSCNPHKILLVEKGKPVEKRHC
                     PKDKTGVCVNCKPTCAITTGFSGAGAFSDGKLSLSYQVGGELPDLIGEDFAQELIDYT
                     DKIYLEFGADPKVEGVYEGPDIKDIRKRAIQAGLQLVDCPIRHLGTEKAQQLYLNIQN
                     YLADAGVEMLFNTECENIILEDSVCKGVRLREKDGTRDVLAKQVVIATGRRGADWLEK
                     LCAEHNIAHKPGTVDIGVRVECRNEVMEKINQVLYEGKLIGYPAPWHNKVRTFCQNPG
                     GFVAQENYDNDLAVVNGHSFKEKKSNNTNLAILVSHNFTEPFNQPIAYAQKVGELTNM
                     LGAGHILVQRYGDILDGKRTWAKELARTNVRPTLKDAVAGDITAAMPYRAMVNIIEFI
                     KMMDEVVPGFASPETLLYSPELKFYSNKVKMDTDLETNIAGLHCLGDSSGWTRGLMMA
                     SVMGVLMGRKLMEKHGF"
BASE COUNT          810 a         1197 c         1139 g          702 t
ORIGIN      
        1 accgctgggg catcaaggtc aaccgcgttg agctgaaaaa catcgagcct ccggtggaaa
       61 tccgccaggc tatggaaaag cagatgaagg ccgaccgcga gaagcgcgcc tcgatcctgc
      121 tggccgaggg cgaaaagcag gccgccatca cccgtgccga gggcgaaaag gagtctgcca
      181 tcctgcgcgc cgaggctgtc aagcagcagc gcatccgcga ggccgaaggt gaagccgagg
      241 ccctgctgac cgtgcagaaa gcccaggccg acgccatccg catgatcaac gaggcgaacc
      301 ccaaccacaa ctacctggcc ctgcgcagca tggaagccat ggagaaggtt gccgacggca
      361 aggccacgaa gctgatcgtg cccagcgata tgcagaacct ggccgccacc gtctccgcca
      421 tcaaggagat ttccggcgaa gtgaccgaat aaaattcagc attctgtttt ccctgcccgc
      481 ggtgttctgc cgcgggcagc ttttgttgtg gtggtcgatg cacgcatccg ccctgcacag
      541 taaaaatccc accaaatctt cttgcgtttt ccacaaaatc aggttataat ataagcagat
      601 tagtatgcca ttttgcgcaa actatacaaa aataattgtg aggtgtatca gtatgaagga
      661 acctatggtc attactatgg cgcgtgagta cgcatccggc ggcagcgaaa tcgcacaggc
      721 cgtcgcagat aagctgggta tccccctgta caataaggag ctgatcaccc ttgccgccaa
      781 aaaaagcggc ctgaccgagg aggccatcgc cgccagcgag aaccagcgca gcggcagcct
      841 gatttacagc ctgtacatga tgggcaacac catgcccctg gccgaccagg tctatatttt
      901 gcagagcaac gtcattaaag agctggccgc ccagggttcc tgcgtcattc tgggccgctg
      961 cggcgactat gtcctgcggg agcgcccgaa tgtgctgcgc acctttgtct atgcgccgct
     1021 ggatgcccgc atcgggttcg ccaagagccg ccccgacgcc aaggatatgc ccgaccgcct
     1081 gtgggagacc cagctggcca agcatgaccg cgcccgcgcg agctactaca actactacac
     1141 cgagaaccgc tggggcgagg ccaaaaacta cgacctgtgc ttgaatgcca ccctgggccg
     1201 cgaagcctgc gccgacctga tcgtcgcggc agccaaggcc atgcaagcgg ccaacaagta
     1261 aaaaccggaa ggagccaccc gccatgctgc gctatgtcaa aagcggaatg atgctgttga
     1321 gcatcgccta tatcgtcatc ggtatgatgc tgctcatcat gccccagacc agcctgctct
     1381 ggatctgcta cgctttcggg gccgtcgtgc tcatcaccgg catcgtctgc ctgatccagt
     1441 acgcccgcat ccgcggcaca ggctttactg cgccgttcat gctggtgggc ggcgtcatca
     1501 cggcggggct gggcatcttt acgctggcga aaccgcaggt ggtggcgagc tttctcccca
     1561 tcgtgttcgg catcttcatc gttgtggacg gtttgagccg catcggcagc gccatcgacc
     1621 ttgccaagcg caaggggcag aaatggtggg tgctgctgct gctcagcatc gtctctgttg
     1681 cgctgggcat actgctggtg ctgcatccgt ttggtgcggc tgtcagcgtc gtgatggtgt
     1741 gcggtattct gctgatcgtc gaaggtgcga tgaacttagg ttgcattctg tatgctgcca
     1801 tggagctgcg cacgctggac cgcatggcga ctgccgccgc caacgctgcc atgtccgcca
     1861 tcggtgacgc cctggacgaa gccgaagccg ccgagtcccc cgccgacacc ccggtcgttt
     1921 acgacgccga gagcaaggac gtgtcggagg acggtgaata accgttccgt gacaccgtag
     1981 gggcgcattc tatatgcgcc cgcagcgccg tgccatcagc aaacgggcac gggcggatat
     2041 agaatccgcc cctacaagct ccgtttgccc tcattctgtt tcccgcgcga acgttcgcga
     2101 gttattttta aaggagagaa cccccatcca tgtatgacct tatcatcgta ggcgccggcc
     2161 ctgccggtat tttcaccgcg ctggagctgc tgcgcaaaag ctgcaacccc cacaagatcc
     2221 tgctcgtcga aaagggcaag cctgtggaaa agcgccactg ccccaaggat aaaaccggcg
     2281 tctgcgtaaa ctgcaagccg acctgtgcca ttaccaccgg cttttccggc gcgggcgcat
     2341 tctcggacgg caagctgtcc ctgtcctacc aggtcggcgg cgagctgccc gacctgatcg
     2401 gcgaggattt tgcccaggag ctcatcgact acaccgacaa aatctacctc gagttcggcg
     2461 ctgaccccaa ggttgagggc gtctacgaag gccccgatat caaggacatc cgcaagcgcg
     2521 ccatccaggc gggcctgcag ctggttgact gccccatccg ccatctgggc accgagaagg
     2581 cccagcagct ctatttaaac atccagaact atctggccga cgcgggcgtc gagatgctgt
     2641 tcaacaccga gtgcgagaac atcatcctgg aggattccgt ctgcaagggc gtccgcctgc
     2701 gcgagaagga cggcacccgc gacgtgctgg cgaagcaggt cgtcattgcc acgggccgcc
     2761 gcggcgcgga ctggctggag aaactctgcg ccgagcacaa catcgcccac aagcccggca
     2821 ccgttgacat cggcgtccgc gttgagtgcc gcaacgaggt catggagaag attaatcagg
     2881 tcctgtacga gggcaagctg atcggctacc cggccccgtg gcacaacaag gtgcgcacct
     2941 tctgccagaa ccccggcggc tttgtcgcgc aggagaacta cgacaatgat ctggcggtcg
     3001 tcaacggcca cagcttcaaa gagaagaaga gcaacaacac caacctggcg atcctcgtca
     3061 gccataattt taccgagccg ttcaaccagc ccatcgccta cgcgcagaag gtcggtgagc
     3121 tgacgaatat gctgggcgcg ggccacattc tggtgcagcg ctacggtgat atcctggacg
     3181 gcaagcgcac ctgggccaaa gagctggccc gcaccaacgt ccgcccgacg ttgaaggacg
     3241 ctgtggccgg tgatatcacc gccgccatgc cctaccgcgc catggtcaac atcatcgagt
     3301 tcatcaagat gatggacgag gtcgttcccg gttttgccag ccccgaaacg ctgctgtaca
     3361 gccccgagct gaagttctac tccaacaagg tcaagatgga caccgacttg gagacgaaca
     3421 tcgcgggtct gcactgcctg ggcgattcct ccggctggac gcgcggcctg atgatggcct
     3481 ccgtcatggg cgtcctgatg ggccgcaagc tgatggagaa gcacggattt taacggatac
     3541 gataagggga gagcgcccga cgtgggtgct ctcccctgtt tttgtgcagc cgctgttgca
     3601 ggggggcggc gttgcggggt tgcgagcgtg ggcccacggt tgagcgtgcg aaaccgggga
     3661 gcgagcaatg ttcgtcccat gggacgaaca accgcacgag ctggtttgcg cagcaaactg
     3721 gcggcgctcg ttgtgcgccg ccaagcggcc gacgccccgg gcggtgtttc ctcaagcggg
     3781 gcctcccctg cggccgcccc cttccgcaaa atttttacaa gaattttcaa aaaccgccct
     3841 ccgtgtga
//
LOCUS       DBMDSQ010000199         3835 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_5502_length_3835_cov_1.752812, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000199 DBMDSQ010000000
VERSION     DBMDSQ010000199.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 3835)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3835)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3835
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5502_length_3835_cov_1.752812"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..143)
                     /locus_tag="ACZPAX_07465"
     CDS             complement(<1..143)
                     /locus_tag="ACZPAX_07465"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017825909.1"
                     /note="catalyzes the isomerization between
                     2-isopropylmalate and 3-isopropylmalate in leucine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HIR1113776.1"
                     /translation="MGMTMTQKILAAHSGEAAVTAGQLINAKLDVVLGNDITTPVAIN
                     EFE"
     gene            complement(285..>986)
                     /locus_tag="ACZPAX_07470"
     CDS             complement(285..>986)
                     /locus_tag="ACZPAX_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009987793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HIR1113777.1"
                     /translation="ERTPYAGSLVFAAFSGSHQDAIAKGMTWRKEKQLHQWTCPYIPI
                     DPHDIGRTYDADVIRINSQSGKGGIGFLLQQNYGYNPPPKMREDLGYRVKDVSDHEHR
                     ELSVKEVLGVFESSYLNHTHPLNVPEAHFIQNSDGSITASVVITSGGSTIKCNATGNG
                     RLDAVATAIEQQTGMHFTLVHYSEHALDSDTNSRACSYVGLKWASGEESWGCGTHTDI
                     IVAGIKALVSAINNK"
     assembly_gap    987..996
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<997..1959)
                     /locus_tag="ACZPAX_07475"
     CDS             complement(<997..1959)
                     /locus_tag="ACZPAX_07475"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015536897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HIR1113778.1"
                     /translation="MMDASKYNVGYYPPPVEPGHVYEWPQKDHIEQAPAWCSVDLRDG
                     NQSLIVPMNLEEKLEFYDMLVKIGFKEIEVGFPAASETEYEFLRTLIDGNRIPQDVTV
                     QVLTQCRDHIIRKTFEAVKGAPRAIIHFYNSTSVAQREQVFKKSKEEIKKIAVDGAKL
                     VKQLSEEYEGNFLFEYSPESFTGTEPEYAVEVCNAVLDVMQPTPDRPMIINLPVTVEM
                     SMPHIYANQIEWCSKHLKYRDSVILSTHPHNDRGCGVADAELAVLAGAQRIECCLFGN
                     GERTGNVDAITLAMNMYSHGVDPHLDFSNMPEICEVYERVTRMHV"
     misc_binding    complement(2040..2289)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2559..3695
                     /locus_tag="ACZPAX_07480"
     CDS             2559..3695
                     /locus_tag="ACZPAX_07480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113779.1"
                     /translation="MLYQKYKPLASRVYCAGLALLLVLGEVFSSNLQSTLPTFSRVAR
                     LGLTGFAVLLLAGKIVLLTEYETQLQKIIVAVVLVYTAFSAWYGGDQWFFLAALVGLG
                     AKDVDLQTALRVYLATAVAGLLLVQALHFITPLMPYNFYCRNWDFGYGHYNGFGARLV
                     GVFFAWGWLRHDRMRAFDWAGLAALAIFTYKVPGSRGAFGGMAVLFGLFLVQKFLPKL
                     FDNKIFYGFSIALPVALAAFSLYAGYVYNPEWPYERMALLLLSIALSGRFEIWHNVFW
                     QAPLSLLGGLPTDGDEHHAIDNTFLAVPMNKGIIGAILVAAVFLLLLWRLAKKHRATE
                     VICLVALTLYLFMENKPFLVSANPFLLMLPVVFFGSPDSKNQTE"
BASE COUNT          674 a         1036 c         1153 g          962 t
ORIGIN      
        1 ttttcaaatt cgttgatggc caccggggtg gtgatatcat tgccgagcac tacatcgagc
       61 ttggcgttga tgagctggcc cgccgtgacg gctgcttccc cgctgtgggc cgctaagatt
      121 ttttgggtca tggtcatgcc catggttgtg tacctccgtt ttaggatcat ctcaccgtag
      181 gggcggggca tgccccgccc tcggccttgc ctttacgtgc cgcctgccct tatgttgagg
      241 gccggacaag tccggcccct acccggcagt gcgttgtagg gtgtttactt attgttgatc
      301 gcgcttacca gcgccttaat gcccgccacg ataatatccg tatgcgtgcc gcagccccag
      361 ctctcctcgc cggaggccca tttcaggccg acgtaggagc aggcgcggga gttcgtatcg
      421 gaatcgaggg cgtgctcact gtaatgcaca agcgtgaaat gcatcccggt ctgctgctcg
      481 atggccgttg ccacagcgtc caggcggcca ttaccggtgg cgttgcattt gatcgtgctg
      541 ccgccgctgg tgatgacgac gcttgccgtg atgctgccgt ctgagttctg gataaagtgc
      601 gcttccggca cattgagcgg gtgggtgtgg ttgaggtagc tgctctcaaa gacgcccagc
      661 acttctttca cgctcaactc gcggtgctcg tggtcgctga cgtccttgac gcggtagccc
      721 agatcctcgc gcattttcgg cggcgggttg taaccgtagt tctgctgcag caggaagccg
      781 atgccgccct tgccgctctg ggagttgata cggatgacgt cggcatcgta ggtacggcca
      841 atgtcgtggg ggtcgatggg gatgtagggg caggtccact ggtggagctg cttttccttg
      901 cgccatgtca tgcccttggc gatggcgtcc tgatggctgc cgctgaacgc cgcaaagacg
      961 agggagcctg catacggcgt gcgctcnnnn nnnnnngacg tgcatccggg tcacgcgctc
     1021 gtagacttcg cagatttcgg gcatattgct gaagtcaagg tgtgggtcca cgccgtggct
     1081 gtacatattc atagccagcg tgatggcgtc cacgttgccg gtgcgctcgc cgttgccgaa
     1141 gaggcagcac tcgatgcgct gagcaccagc cagcacagcc agctcggcat cagcgacgcc
     1201 gcagccgcgg tcgttgtggg ggtgggtaga aagaatcacg ctgtcacgat atttcagatg
     1261 tttggagcac cattcaatct gatttgcgta gatgtggggc atgctcatct cgaccgtgac
     1321 cggcaggtta atgatcatgg ggcggtcggg ggtgggctgc atgacgtcga gaacggcgtt
     1381 gcagacttcc acggcgtact ccggctcggt cccggtgaag ctctcggggc tgtactcaaa
     1441 caggaagttg ccctcgtact cctcgctcag ctgcttgacg agcttagcgc cgtcaacggc
     1501 gatcttcttg atttcctctt tggacttctt gaacacctgc tcgcgctggg cgacactggt
     1561 ggagttgtag aagtggataa tggcccgggg cgcgcccttg acggcctcga acgtcttgcg
     1621 gatgatgtgg tcacggcact gggtcagcac ctgcacggtc acatcctggg ggatgcggtt
     1681 gccgtcgatc agcgtgcgca ggaactcgta ctcggtctcg ctggcggcgg ggaaaccgac
     1741 ctcgatttcc ttaaagccga ttttgaccag catatcgtaa aattcaagtt tttcttccag
     1801 gttcatcggc acgatcaggc tctgattgcc atcgcgcaga tccacactgc accaggccgg
     1861 ggcctgctcg atatggtctt tctggggcca ctcatagaca tggccgggtt cgaccggcgg
     1921 cggatagtag cctacattgt acttggatgc gtccatcata ataaaaatgc ctccttcaaa
     1981 gcgttcggtt cgcgctttgg ggaggtaaaa ataaaccgtc ccccaaaggc gaaagacctt
     2041 tgagagacgg gagcaaaatt cacgttgctg tacccgcggt accactctca ttgccgttga
     2101 aacggccgct ctgtacgatc agcttttcag cgaatcgcgc ctgcatgata acggtcaggt
     2161 tccgtccgcg cctacctgtc agcgcggctg ctccggggcc agtatacaac atctgccata
     2221 ccgccttgca ccgaccggcg gctctctgca atgggatgga tgctgctttt tccccttcac
     2281 tgcatttgca atggttctat tttagcggat tcttggggga atgtcaagta ttatttttgc
     2341 aaaatttgca aggatgtgtt ttgtgtttat caacattatt tatgtagggg ccgggtatgc
     2401 ccagcccccg cgtgaaggat aagcaggcac ttatgggaaa gctgcgggcc gcgtatgcgc
     2461 ggcccctacc gtgtgccgct acttttacag ggttttcttt ttatcgttta tatgatatac
     2521 ttataatgat ttttatttaa ttccgaaagg attcttctat gctttaccaa aaatacaaac
     2581 cgctggccag ccgcgtgtac tgtgcggggc tggcgttatt gcttgttttg ggcgaggtat
     2641 tcagttccaa ccttcagtct acgctgccga ctttcagccg ggtggcgcgg ctggggctga
     2701 ccgggtttgc cgtactgctg ctggcgggca aaatcgtatt gctgaccgag tacgagacgc
     2761 agctgcaaaa aatcatcgtg gccgttgtgc tggtctacac ggcgttttcg gcctggtacg
     2821 gcggcgacca gtggtttttc ctggctgcgc tcgtcgggct gggggccaaa gatgtggatt
     2881 tgcagacggc cctgcgggtc taccttgcca cggcggtggc cgggctgctg ctggtgcagg
     2941 cgctgcactt tatcacgccg ctgatgccct acaatttcta ctgccgcaac tgggatttcg
     3001 gctacggcca ctacaacggt ttcggtgcgc ggttggtggg cgtgttcttc gcctggggtt
     3061 ggctgcggca cgaccggatg agagctttcg actgggccgg gctggccgcg ctggcgattt
     3121 ttacctacaa agtacccggc agccggggcg cgttcggcgg tatggccgtg ttgttcgggc
     3181 tgtttctggt gcagaaattt ctgccgaagc tgtttgacaa caaaattttc tacgggtttt
     3241 cgattgccct gccggtggcg ctggcagcgt tcagcctata cgctggatac gtttacaacc
     3301 ccgaatggcc ctatgaacgg atggcactgc tgctgttgag cattgcactg tcgggtcggt
     3361 ttgagatttg gcacaatgtt ttctggcagg ccccgctctc gctgctgggc ggcctgccca
     3421 ccgacggcga cgagcaccac gccattgaca acacgttttt ggcggtgccg atgaacaaag
     3481 gcattatcgg tgccattctg gtggccgccg tgttcctgtt gctgctgtgg cggttagcga
     3541 agaagcaccg cgccaccgag gtcatctgcc tggtggcgct gacgctgtat ctcttcatgg
     3601 agaacaagcc gtttttggtg tcggccaatc cgtttttgct gatgctgccg gtggtgtttt
     3661 tcggcagccc cgattccaaa aatcaaaccg aatgaaccct cttaggctgg atcgtatcac
     3721 attttcgcgg aaatgcggta tcgatcctgc catttttgtg ctatactggg tgtattctaa
     3781 aacaaaggag tctccccgaa tgcagacgaa aatcgcattt tttgatatcg acggc
//
LOCUS       DBMDSQ010000200         3775 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_5589_length_3775_cov_2.710942, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000200 DBMDSQ010000000
VERSION     DBMDSQ010000200.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 3775)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3775)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3775
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5589_length_3775_cov_2.710942"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..691
                     /locus_tag="ACZPAX_07485"
     CDS             <1..691
                     /locus_tag="ACZPAX_07485"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014663.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="5-oxoprolinase subunit C family protein"
                     /protein_id="HIR1113780.1"
                     /translation="LKIGVAASDLRSYLAVGGGIATPPVLGSRATDVKCGIGGLKGRK
                     LAKGDTLPTGVCDTAALWRIITAKHLDRPLRDKAVCRGTHSVRVQGAQTLPLLRTVPG
                     PQDAAFTAQGLHDFTHGIYTLTPNCDRMACKLAGPPLAMHHGADILSDSIVPGSVQVS
                     ADGQPIVMLADHQTTGGYAKIATVISADLPALAQLRPGQRVCFTFVTPAQAVQAARQQ
                     TALWQQVRNRL"
     gene            707..1498
                     /locus_tag="ACZPAX_07490"
     CDS             707..1498
                     /locus_tag="ACZPAX_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002581102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative hydro-lyase"
                     /protein_id="HIR1113781.1"
                     /translation="MKYSAFPPAEVRALIRAGKLDAPTTGYCDGYAQGNLVVLPKALA
                     WDFLLFCQRNPKACPLLEVADAGERSFSQFAPGSDIAQDIPRYRIYQNGELIEETTDV
                     TRYFEERDDLVSFLIGCSFSFESALLDADIPVRQIEEGVNVPMYNTNIPCAPAGVFSG
                     NMVVSMRPIPYAQVPAAVAITAGMPRVHGAPVQIGTPEAIGITDLAHPDYGDAVTIRP
                     GETPVFWPCGVTPQAVVMHSKPEFCITHAPGHMFVTDVKNTALKF"
     gene            1513..2292
                     /locus_tag="ACZPAX_07495"
     CDS             1513..2292
                     /locus_tag="ACZPAX_07495"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LamB/YcsF family protein"
                     /protein_id="HIR1113782.1"
                     /translation="MPTIDLNCDLGESFGAYTIGMDDEILPYVTSANIACGFHAGDPS
                     VMKKSVLLCKKYGVQVGAHPGLPDLQGFGRRKMAISPAEAEADVVYQIGALKAFCDAA
                     GVPLHHVKPHGALYNMAAKDPALAAAICRAVQAAAPGAVLLALSGSEMIKAAHTIGLP
                     VASEVFADRGYQPDGTLVPRGTPGAMVEDEDTAIVRVLQMAKQGTVQANDGSTVTLQA
                     DSVCIHGDGPKALAFVQKISTALPAAGVEVRTFTATTSGAT"
     gene            complement(2426..2752)
                     /locus_tag="ACZPAX_07500"
     CDS             complement(2426..2752)
                     /locus_tag="ACZPAX_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005946353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HIR1113783.1"
                     /translation="MTAQPFDAVVDALYGTGFHGSLRPAGLAACALINRQRENGAFVL
                     AVDLPSGVNTDTGEVAEGAVHADLTVTFDSYKPLHIAPSSASFCGEILCADIGIRDEW
                     HPTDNA"
     gene            2928..3173
                     /locus_tag="ACZPAX_07505"
     CDS             2928..3173
                     /locus_tag="ACZPAX_07505"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019887.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR1113784.1"
                     /translation="MTGEHWLDFTATGKDKGLHLLCAHFGIPQENTYAFCDSYNDAPM
                     LRAAAHPYLMNIAPADLKAEFATQIDNVAGVLKTLGH"
     gene            3258..3671
                     /locus_tag="ACZPAX_07510"
     CDS             3258..3671
                     /locus_tag="ACZPAX_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005945636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SseB family protein"
                     /protein_id="HIR1113785.1"
                     /translation="MKMKEDENRRLNQALEDAVQGLQKAPTQEQLAHTLTVVRRVMQA
                     GGQWIAAVETTPGASAALRPKIVQTADGGRWWYAFTSFDEQMRSPDAVKSTFWADINS
                     LFDAALAAEEIQGIILNPWHCTLQLDKTLIRIIKP"
BASE COUNT          759 a         1140 c         1157 g          719 t
ORIGIN      
        1 actgaaaatc ggcgtggctg cgtctgattt gcgcagctac ttggctgtgg gcggcggcat
       61 tgccaccccg cccgtgctgg gtagtcgcgc caccgatgtg aaatgcggca tcggcggctt
      121 gaaaggccgc aagctggcaa aaggcgacac gctccccacc ggggtatgcg ataccgccgc
      181 gctctggcgg atcattacgg ccaaacatct ggaccgcccg ctgcgggaca aggccgtgtg
      241 ccggggcacc cattctgtgc gcgtgcaggg ggcacagact ttgccgctgc tgcgcaccgt
      301 acccggcccg caggatgccg cgttcaccgc gcaagggtta catgatttta cccacggcat
      361 ctacacattg acacccaact gtgaccgcat ggcctgcaag ctggcgggcc cgccgctggc
      421 aatgcaccat ggcgcggaca tcctctccga tagcattgtg cccggttcgg tgcaggtctc
      481 cgccgacggt cagcccatcg tgatgctggc cgaccaccag accaccggcg gctacgccaa
      541 aattgcaacg gtcatctccg ctgacctgcc cgcgctggcg cagctgcgcc caggtcagcg
      601 agtatgcttc acctttgtga cccctgccca ggccgtacag gccgcccggc agcagaccgc
      661 cctgtggcag caggtacgaa atcgtttgta aaaggaggtg tgttgtatga aatactccgc
      721 atttcccccg gctgaggtcc gtgcgctgat ccgggcgggt aagctggatg cccccactac
      781 cggctattgc gacggctatg cccaaggtaa cctggttgtg ctgcccaaag cgctggcatg
      841 ggactttctg ctattctgcc agcgcaaccc caaggcctgc cccctgctgg aagtggcaga
      901 tgccggggag cgcagcttct cccaatttgc gccgggcagc gatatcgcgc aggacatccc
      961 gcgctaccgc atttaccaaa atggcgaact aatcgaagaa actaccgatg tgacgcggta
     1021 ttttgaagag cgggacgatt tggtcagctt ccttatcggg tgcagcttct cgtttgagtc
     1081 ggccctgctg gacgccgaca tcccggtgcg ccagatcgag gagggcgtca acgtgccgat
     1141 gtacaacacc aacattccct gcgcgcctgc gggggtgttc agcggcaaca tggtcgtcag
     1201 tatgcggccc atcccgtatg cgcaggtgcc cgcggcggta gccatcacgg caggcatgcc
     1261 ccgcgtacac ggagcaccgg tgcagattgg tacaccggag gctatcggca ttactgacct
     1321 agcccacccc gactacggcg acgccgtgac gatacgcccg ggcgaaacgc ctgtattctg
     1381 gccctgtggg gtcacgccgc aggccgtggt catgcactca aaacccgagt tttgcatcac
     1441 ccacgcgccg ggacatatgt ttgtgaccga tgtgaaaaac acggcgctga aattttaaga
     1501 gggggctttg taatgccaac cattgatttg aactgcgatt tgggcgagag tttcggcgcg
     1561 tacaccatcg ggatggacga tgaaattcta ccctacgtga ccagcgcgaa tattgcctgc
     1621 gggtttcacg cgggcgaccc gtccgtgatg aaaaagagcg tattgttgtg caaaaaatac
     1681 ggcgtgcagg tgggcgcaca ccccggcctg cccgacttgc agggctttgg ccgccgcaaa
     1741 atggcaatca gtcccgctga agctgaggcc gacgtagtgt atcaaattgg cgcactgaaa
     1801 gccttctgcg atgcggcggg tgtgccgctg caccacgtca agccccacgg cgcactctac
     1861 aacatggctg ccaaagaccc ggcgctggcg gcggccatct gccgcgccgt gcaggctgcg
     1921 gcccccggtg cggtgttgct ggccctgtcc ggcagcgaaa tgatcaaggc cgcccacacc
     1981 atcggcctac cggtcgccag cgaggtattt gccgaccgcg gctaccagcc ggacggaacc
     2041 ttagtgcccc gcggtacgcc tggtgcgatg gttgaggacg aggacactgc catagtgcgg
     2101 gtcttgcaga tggcaaagca gggcactgta caggccaacg atggcagcac ggtaactttg
     2161 caggccgatt ccgtctgcat ccacggcgac gggccgaaag ctcttgcctt cgtacagaaa
     2221 atctccaccg ccctgcccgc tgcaggagtg gaagtcagaa cgtttaccgc gaccaccagt
     2281 ggggcaacgt agcccgcaac aaaatgtggc atcactagct gtgggggcga ccggactttt
     2341 gcgattacgc gctgctggtc tttgccatca agagcttaca agaaaataag agcagggcgg
     2401 agtagggaaa tgctccgccc tgcttttatg cgttgtctgt tggatgccac tcatcccgga
     2461 tgccgatatc ggcacagagg atctcgccgc agaaggacgc ggagctgggg gctatgtgca
     2521 ggggcttgta actgtcaaat gtcacggtca ggtccgcgtg gacggcacct tcggccacct
     2581 cgcctgtgtc ggtgttgacg ccgctgggca ggtcaactgc cagcacaaat gcgccgtttt
     2641 cccgctggcg gtttatcagt gcgcaggcgg ccagcccggc cgggcgcaga ctgccgtgga
     2701 acccggtacc gtacagggcg tctaccacgg cgtcaaaggg ctgtgccgtc aaggccgcag
     2761 cgtcacggta tcacgctcga tgaggatgag atcgcctaca tcgcgctgca tctgggcggt
     2821 gcgctggaaa cgcagaagga actggcaaac cgtgtgcctg ccgtgctgta ctgccccagc
     2881 tactacaata atggacagca gcccggcagg atcgcatcgg tgcagccatc acgggcgagc
     2941 actggctgga ttttaccgca acgggtaagg ataaggggct gcatttgctg tgcgcgcatt
     3001 tcggtatccc gcaggaaaat acatacgcct tctgtgacag ctacaacgat gcccccatgc
     3061 tccgtgcggc ggcgcacccc tacctgatga acattgcgcc tgccgattta aaggcagagt
     3121 ttgccacgca gatcgataac gtggcaggtg tgctgaaaac acttggacac tgaataacgg
     3181 cttttacggc ttttctttac aagcgcggcg aatccttcta taatggcgct atacactatg
     3241 aatagcgagg aatttccatg aagatgaaag aagatgagaa ccggcggctc aaccaggcgc
     3301 ttgaggatgc cgtgcagggc ctgcagaagg cccccacgca ggagcaactt gcccataccc
     3361 tgaccgttgt gcgccgcgtc atgcaggcag gcgggcagtg gatcgccgcc gtggagacaa
     3421 cgcccggcgc gtccgcagcg ctgcgcccca aaatcgtgca gaccgccgac ggcgggcgct
     3481 ggtggtatgc cttcacctcg tttgacgagc agatgagaag ccccgatgcc gtgaaatcta
     3541 cattctgggc tgacataaac agcctgtttg acgccgcgct ggctgcggag gagattcagg
     3601 gcatcatcct gaatccgtgg cactgtacac tgcagctgga taaaacgctg atccgcatca
     3661 tcaagccata aaagcagggg agagggtaag ttcgttctgt tgcgattcca ggagtcggtg
     3721 ctgttggttc ctatctgctg tcggtgctat cggtgcccgt ccagccggtt ttcag
//
LOCUS       DBMDSQ010000201         3749 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_5640_length_3749_cov_2.023849, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000201 DBMDSQ010000000
VERSION     DBMDSQ010000201.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 3749)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3749)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3749
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5640_length_3749_cov_2.023849"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..910
                     /locus_tag="ACZPAX_07515"
     CDS             <1..910
                     /locus_tag="ACZPAX_07515"
                     /inference="COORDINATES: protein motif:HMM:NF019161.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HIR1113786.1"
                     /translation="VDPACLDMYCYTDGSMWGSFSGPSTSVGGDSISHVYGYWYNYDE
                     NGEKNFVIHLLGNQDADGTARPTDVDGGADADVFIFDTDHGAYSMEASLSYGVYGGAF
                     TRNINIYGQPYAPAKSLTIDASNLRTFYTGDTFNPAELVVTATRANGAEESIWGGRLT
                     FTGYDSDTTGTKTVTASFLGATATFEVQVEDLVTEQYTGSYELVQGETPTATDAVLLV
                     DYSHKVCTLTAADGSASITGTLVDAQDNALTMTLNGSDALTVPITEGEDGSKQLTIPA
                     HDEIVSGWGNSTTYSINEAVVTLAAE"
     gene            1020..3440
                     /locus_tag="ACZPAX_07520"
     CDS             1020..3440
                     /locus_tag="ACZPAX_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015537136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HIR1113787.1"
                     /translation="MQNTELLLKKLTLEEKCALLSGAETFKTRGMPEHGIPQIWLSDG
                     PHGLRKQAGESDHLGLNPSVPATCFPTASAVANSWDAALGEEIGAALGEEAAAQEVSV
                     VLGPGLNMKRNPLCGRSFEYFSEDPYLAGKLAAGYIRGIQSKGVAACPKHFAVNSQET
                     RRMASDSIVDERTLREIYLTGFEIAVKEGHPRSIMSSYNLVNGTYANENKHLLMEILR
                     GEWGFDGAVITDWGGINDHALGVKNGSTLEMPAPGGDSVRELLAAVESGKISESDIDA
                     RLSELLPLVFDTKAALDAAPREFDAAAHHALARRAAAESLVLLKNEGALLPLAAGSKV
                     AVIGDFAKNPRYQGAGSSMVNSTQVDVLLDKLIDSELNVIGYQQGFDRHGKPDAALQK
                     SACELAAQANTVILCMGLDEIAESEGLDRSNLRLAQNQVDLLQAVAAVNPKIVVVLYS
                     GSVVETPWLDNCQALLYAALGGQAGAGAVADALAGKVNPCGKLAETWPLAYADVPSAA
                     DFATRRKTVEYREGLYIGYRYFTTAEKAVRFPFGYGMSYTTFAYSDMAADEQGVSLTV
                     TNTGSVAGTEIVQLYVAKKSSELFRPAKELKGFARVTLAPGEKQRITITLDDKAFRFW
                     NVKANRWEIEGGEYELLVGASVEDIRLCEKISVHGTATVHPYEDRDLDCYYKGDVLNV
                     SDADFEKLLGHPIPNGKTKIDRNLTLGELNHARSPLGWLVWLVLTILLDVSYKRGKPD
                     LNILFQYNMPLRALAKMTNGAISMGMVDGIVMELQGFWILGLVRVIYEAIKNVVLNAQ
                     MEKRLRGA"
     gene            3454..>3749
                     /locus_tag="ACZPAX_07525"
     CDS             3454..>3749
                     /locus_tag="ACZPAX_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006735322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113788.1"
                     /translation="MQFWNNFAAKHPAAAKWVREGGLFVIVSNLITVFKYLLLQFLPA
                     AFSSLPVVDFGWPGVDVTLFGETFKWNILGYDAAHGGLPYFCAYMIAMVIGECI"
BASE COUNT          784 a         1038 c         1162 g          765 t
ORIGIN      
        1 cgtggatccg gcctgtctgg atatgtactg ctacaccgac ggttcgatgt ggggttcctt
       61 cagcggtccc agcacctccg tgggcggcga ctctatctcc catgtttacg gctactggta
      121 caactacgat gaaaacggcg agaagaactt cgtcattcat ctgctgggca accaggacgc
      181 tgacggcact gcgcgcccca ccgatgtgga tggcggcgca gatgcggatg tcttcatctt
      241 tgacaccgac cacggcgcat acagcatgga agccagcctg agctatggcg tttatggcgg
      301 tgcgttcacc cgcaacatca acatctacgg tcagccctat gcccctgcca agagcctgac
      361 gatcgatgcc tccaacctgc gcaccttcta cacgggcgac accttcaacc cggctgagct
      421 ggttgtcacg gctacccgcg ccaacggtgc ggaggagtcc atctggggcg gtcgcttgac
      481 cttcaccggt tacgacagcg acaccacagg caccaagacc gtgacggcct ccttcctggg
      541 tgcgaccgct acgtttgaag tgcaggtcga ggatctggtc accgagcagt acacgggcag
      601 ctatgagctg gtgcagggcg agacgcccac cgcaacggat gccgtgctgc tggtggatta
      661 ctcccacaag gtctgcacgc tgactgccgc cgacggctct gcatccatca ccggcacgct
      721 ggtggatgcg caggacaatg cccttaccat gaccctgaac ggcagcgatg ccctgacggt
      781 gcccatcacc gagggtgagg acggcagcaa gcagctgacc atccccgccc acgacgaaat
      841 cgtctccggc tggggcaaca gcaccaccta ctccatcaac gaggctgtgg tcacgctggc
      901 ggcagagtaa gcgttcattg cccctgatga agcataacgg tacttgctga atgaaagccg
      961 cgtctgatgt ctgcttgcag gcgttggatg cggcttttct tatgagaagg gaaaaccata
     1021 tgcaaaatac agaacttttg ctgaaaaaat tgaccttgga agaaaaatgc gccctgcttt
     1081 ccggcgcgga aaccttcaaa acgcggggta tgcccgaaca cggcatcccg cagatctggc
     1141 tgtcggacgg tccccacggc ttgcgcaagc aggcggggga gagcgaccac ttggggctga
     1201 atcccagtgt tcccgctacc tgcttcccca cggcgtcggc tgttgcaaac agctgggatg
     1261 ccgcgctggg cgaggaaatc ggtgcagcct tgggcgagga agccgctgcg caggaagtgt
     1321 cggtggtgtt gggaccgggg cttaacatga aacgtaaccc gctgtgcggg cgcagctttg
     1381 agtatttctc cgaggacccg tatctcgcgg gcaagcttgc ggcaggatat attcgcggca
     1441 tccagtccaa gggcgtagcc gcctgcccga agcactttgc cgtcaacagt caggaaacgc
     1501 ggcgtatggc ttcggattcc atcgtggacg agcgcaccct acgggaaatt tacctgaccg
     1561 ggtttgaaat tgccgtcaaa gaggggcatc cccgcagcat tatgtcgtcc tacaatcttg
     1621 tgaacggtac gtatgccaac gagaataagc acctgctcat ggagattttg cgaggtgagt
     1681 ggggctttga tggcgcggtc atcaccgact ggggcggaat caacgaccac gcgctgggtg
     1741 tgaaaaacgg ctccacgctg gaaatgcctg cgcccggcgg ggacagtgtg cgcgaactgc
     1801 tggcggcggt ggaaagcggg aaaatctctg aatctgacat cgatgcccgc ctgtcagagt
     1861 tgctgccgct ggtgtttgat accaaggctg cgctggacgc tgccccgcgt gagtttgatg
     1921 cggcagccca tcatgcgctt gcccgccgcg ccgccgcgga aagccttgtg ctgctgaaaa
     1981 acgagggcgc tttgctgccg ttggcggcgg gcagcaaggt ggctgtcatc ggcgactttg
     2041 ccaaaaatcc gcgctatcag ggcgcaggct ccagtatggt gaactcgaca caggtggatg
     2101 tgctgctgga taagctgatc gactccgagt tgaatgtcat cgggtatcag cagggctttg
     2161 accgccacgg caagccggat gctgctttgc agaaatccgc ctgcgaactg gcagcacagg
     2221 caaatacagt tattttgtgc atgggtctgg atgaaatcgc cgagagtgag gggcttgacc
     2281 gcagcaatct gcgccttgcg caaaatcagg tggatctgct gcaggctgtg gcggccgtca
     2341 acccgaaaat tgtcgtggta ttgtacagcg gcagcgtagt cgaaacgccg tggctggaca
     2401 actgccaagc cctgttgtac gccgccttag gcggtcaggc aggggcgggc gccgttgcgg
     2461 atgcactggc gggcaaggtc aacccctgcg gcaagctggc agaaacatgg ccgttggcct
     2521 acgccgacgt acccagcgcc gccgactttg ccacccgccg caagactgtg gagtaccgcg
     2581 aggggctgta catcggctac cgctacttta ccacggcgga aaaagctgtg cgcttcccgt
     2641 ttggttatgg catgagttac acgacgtttg cgtacagcga tatggctgcc gatgaacaag
     2701 gggtcagcct taccgtaacg aacaccggca gcgtggcagg tacggaaatt gtgcagctgt
     2761 atgttgccaa aaagagcagt gagcttttcc gtcctgcaaa ggaactgaaa ggctttgccc
     2821 gcgtaacgct tgcccccggc gagaaacagc gcatcaccat tacgctggac gataaggcgt
     2881 tccgtttctg gaatgtcaag gcgaaccgct gggaaatcga gggcggcgag tacgagctgc
     2941 ttgtcggcgc aagcgtggag gatatccggc tgtgcgagaa aatctccgtg catggaacgg
     3001 ctaccgtgca tccgtatgaa gatagggatc tggattgcta ttataaaggg gatgtgctga
     3061 acgtttccga tgcggacttt gaaaagctgc tagggcatcc catcccgaac ggcaaaacca
     3121 aaattgaccg caacctgacc ttgggcgaac tgaaccacgc acgcagtccg ctggggtggc
     3181 ttgtgtggct cgtgctgacg atcctgctgg atgtcagcta taaacgcggc aagccggacc
     3241 tgaacatttt gttccagtac aatatgcccc ttcgcgcctt ggcaaagatg acgaacggcg
     3301 ccatcagcat gggcatggtg gacggcattg tcatggaatt gcagggcttc tggatcctgg
     3361 gtcttgtgcg ggtgatttat gaagcgatta agaatgtggt gctgaatgcg cagatggaaa
     3421 agcgtctgcg cggcgcttga tggaggttgc attatgcagt tttggaataa tttcgcggca
     3481 aaacaccccg ccgcagccaa gtgggtgcgc gagggcggtc tgttcgtcat cgtgtcgaat
     3541 ctgattaccg tgttcaagta tctgctgctg caatttttgc ccgctgcgtt cagcagtctg
     3601 ccggtggtgg atttcggctg gcccggcgtg gatgtgaccc tgtttgggga aaccttcaag
     3661 tggaacatcc tcggctacga tgccgcccac ggcgggctgc cgtacttctg cgcgtatatg
     3721 atcgccatgg tcattggcga gtgcatcaa
//
LOCUS       DBMDSQ010000202         3715 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_5706_length_3715_cov_1.521306, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000202 DBMDSQ010000000
VERSION     DBMDSQ010000202.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 3715)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3715)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3715
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5706_length_3715_cov_1.521306"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..359
                     /locus_tag="ACZPAX_07530"
     CDS             <1..359
                     /locus_tag="ACZPAX_07530"
                     /inference="COORDINATES: protein motif:HMM:NF015721.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HIR1113789.1"
                     /translation="QMITKVNPYGHISLTNDYFSGLVEQAAKQCYGIAAMGQAPAESV
                     VRSALRTGSLPPKGVTVTQEGGKLVIALHIKVGFGLNISTITQSITHRVKDEVELATG
                     LKVARIDVYVDDIIAD"
     gene            397..2082
                     /locus_tag="ACZPAX_07535"
     CDS             397..2082
                     /locus_tag="ACZPAX_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005945873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HIR1113790.1"
                     /translation="MITGQTLRDAILSGANNIANQRTRVDELNVFPVPDGDTGTNMSM
                     TIGAARAELEAMPDSCTVAEASKTAASAMLRGARGNSGVITSLLFRGFSKALKDKTEA
                     SAADLAAALQQGVEAAYKAVMKPTEGTILTVSRLAAEKAAECTELDVPAMWDATLQAG
                     QAALDDTPNLLPVLKKAGVVDAGGQGIMLIFEGMKQVFDGGEIIAGAEVAAKPKVSSE
                     AAGKGVFADDLMKVEDIKNGYCTQFLLHKDEGASVARLRAFLESNGDSVVVIEDDDVA
                     NCHVHTSDPGMMLSEAIKYGYLTNFKIENMHEQFLARQKQAKGLEKQAEAEENKTSEF
                     TYAAVDPEREYGFVAVAAGEGLKAVFGDLGVDAVVSGGQTMNPSTEDILAAIQSVPAK
                     TVLVLPNNKNIIMASEQAQKLADRKVIVLPTRTVPQGMTAMLNFDPDLKPEENAVAMM
                     QAADHVSTGLITYAARDSEFDGKPIKKGEIMALENGKIVATGTDLVKMTYRLARSMKK
                     KDTQFITVISGCDVSDEDAEKTTELVRAKCGSSVEVSHISGGQPVYYYMISVE"
     gene            2227..>3715
                     /locus_tag="ACZPAX_07540"
     CDS             2227..>3715
                     /locus_tag="ACZPAX_07540"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HIR1113791.1"
                     /translation="MPTINDSVQYLKGVGPAFAKKFEKLGIVTIRDLLLCYPRKYIDY
                     TQPYTVVSAPYDVDCCVRATVLQKEPPRRITGGRVMNRVLAADDSGILSMTWFNASYA
                     ANNLVVGETYYFEGRIGGTLTRREISHPLVRTEAQVAACPFVAVYPGTDGLPAARHAS
                     CAHAALAFADELTDPLPPELLTHYRMPTKAAAVRGIHAPQSSEELAAARRRLIFEELY
                     LLQIGIFLLRSHGRHKTSAPMHPLDLGPFWRSLPYPPTGAQKRSTEEIVGDLCGEVPM
                     NRLLQGDVGSGKTLVAAAAIWFAAQNGWQSAMLAPTEILARQHAVTLADRLEPFGVNV
                     TLLVGGMKAKEKRIALEAIADGRANLVVGTHAVLTDTVEFKNLGLAIVDEQHRFGVRQ
                     RGLLAGKAQSPHLLVMSATPIPRTLGLLMYGDLDISVLDELPPGRKPIKTWFITGKKR
                     RDMYGFLDKQIAAGHQVYIVCPAIEENEAMGDLQAVTTYYTETACA"
BASE COUNT          759 a         1195 c         1116 g          645 t
ORIGIN      
        1 cgcagatgat caccaaggtc aatccctatg gacatatttc gctgaccaat gactacttct
       61 ccggcctggt cgagcaggcc gccaagcagt gctacggcat tgccgccatg ggtcaggccc
      121 cggccgaaag cgttgtgcgc agcgccctgc gcaccggcag cctgccgccg aagggcgtca
      181 ctgtcacgca ggagggcggc aagttggtca tcgccctgca catcaaggtc gggttcggcc
      241 tgaacatttc gaccatcacc caaagcatta cccaccgcgt caaagacgaa gttgaacttg
      301 ccaccggcct gaaagtcgcc cgcatcgatg tgtatgtgga cgacatcatt gccgactaac
      361 agaattgtat ctgcaaacat gaggtgtaac gaaaccatga ttactggcca gaccctgcgc
      421 gacgccattc tttccggcgc caacaacatt gccaaccagc gcacccgcgt ggatgagctg
      481 aacgttttcc ctgtgcccga cggcgacacc ggcacgaaca tgagcatgac catcggcgca
      541 gcccgcgccg agctggaagc catgcctgac agctgcaccg tggccgaggc cagcaagacc
      601 gctgcctctg ccatgctgcg cggcgcacgc ggcaactccg gcgttatcac gagccttctg
      661 ttccgcggct tctccaaggc actgaaggat aagaccgagg cctccgccgc cgaccttgct
      721 gctgcattgc agcagggtgt tgaggctgcc tacaaggccg tcatgaagcc caccgagggc
      781 accatcctga ctgtctcccg cctggctgcc gagaaagccg ccgagtgcac cgagctggac
      841 gtgcccgcca tgtgggatgc cactttgcag gccggtcagg ccgcactgga cgacaccccg
      901 aacctgctgc ctgtgctgaa aaaggccggc gtcgtggacg ccggcggcca gggcatcatg
      961 ctgatctttg agggcatgaa gcaggtgttc gacggcggcg agatcattgc cggtgccgag
     1021 gtcgccgcca agcccaaagt gtccagcgaa gccgccggca agggcgtctt tgccgatgac
     1081 ctgatgaagg tcgaggacat caagaacggc tactgcaccc agttcctgct gcataaggac
     1141 gagggtgcca gcgtggcccg tctgcgcgca tttttggagt ccaacggtga ctccgttgtc
     1201 gtgatcgagg acgacgacgt ggctaactgc catgtccaca cctccgaccc cggcatgatg
     1261 ctgagcgagg ccatcaagta cggctacctg accaatttta aaattgaaaa tatgcacgag
     1321 cagttcctgg cccgccagaa gcaggccaag ggcctcgaga agcaggctga ggccgaggag
     1381 aacaagacca gcgagttcac ctacgccgcc gttgaccccg agcgtgagta cggctttgtg
     1441 gccgtagctg caggcgaagg gctgaaggct gtgttcggcg acctgggcgt ggacgcggtt
     1501 gtcagcggcg gccagaccat gaacccctcc accgaggata tcctcgccgc catccagagc
     1561 gtgcccgcca agacggtgct cgttctgccc aacaacaaga acatcatcat ggcatccgag
     1621 caggcgcaga agctcgcgga ccgcaaggtc atcgtgctgc ccacccgcac cgtgccccag
     1681 ggcatgactg ccatgctgaa ctttgacccc gacctgaagc cggaggagaa cgccgttgcc
     1741 atgatgcagg cggcggacca cgtcagcacg ggtctgatca cctatgccgc ccgcgacagc
     1801 gagtttgacg gcaagcccat caagaagggc gagatcatgg cgttggagaa cggcaaaatt
     1861 gttgccaccg gcaccgacct ggtcaagatg acctatcgtc tggcccgctc catgaagaag
     1921 aaggacaccc agttcatcac ggtcatttct ggctgcgacg tcagcgacga ggacgccgaa
     1981 aagaccaccg agctggtgcg cgccaagtgc ggcagcagtg tcgaggtcag ccacatcagc
     2041 ggcggccagc ccgtgtacta ctacatgatc tctgtggagt aattcggacg ttataacatt
     2101 aaaaaggcga atttggggat gttccgagtt cgcctttttt gggtatatgg gcgtggctgc
     2161 ggcaaggctg caaagcccct cgccttaatc accctgtaaa aattccacga aaggaggccg
     2221 ccacccttgc ccaccatcaa cgactccgtc caatacctca agggcgttgg ccctgctttc
     2281 gccaaaaaat ttgaaaaact cggcatcgtc accatccgtg acctgctttt gtgctacccc
     2341 cgcaaatata tcgactacac ccagccctac accgtcgtgt ctgcgccgta tgacgtggac
     2401 tgctgcgtca gggccaccgt cctgcaaaag gagccgcccc ggcgcatcac cggcgggcgg
     2461 gtcatgaacc gggtgctggc cgctgacgat tccgggattt tgtccatgac ttggttcaat
     2521 gcgtcctacg ccgccaacaa tctggtggtg ggggagacct actactttga ggggcgcatc
     2581 ggcggcacac tgacccggcg cgagatttcc cacccgctgg tgcgcaccga ggcccaggtc
     2641 gcggcctgtc cgttcgtggc tgtctacccg ggcaccgacg gtctgcccgc cgcccgccac
     2701 gccagctgcg cccacgcggc gctggccttt gccgacgagc tgaccgaccc gctgccgcct
     2761 gaactgctga cccactaccg gatgcccacc aaggccgccg ccgtgcgggg catccacgcg
     2821 ccccagagca gtgaggagtt ggccgccgcc cgccgccgcc tgatttttga ggaactgtat
     2881 ttactgcaaa tcggcatctt tttgctgcgc agccacgggc gccacaagac cagcgccccg
     2941 atgcacccgc tggatttagg gccgttctgg cgcagcctgc cgtacccgcc caccggcgca
     3001 cagaagcgct cgaccgagga aattgtgggg gacttgtgcg gtgaggttcc gatgaatcgc
     3061 ctgttgcagg gcgacgtcgg cagcggcaaa acgctggtgg cggcggcagc tatctggttc
     3121 gctgcacaaa acggctggca gagcgccatg ctggccccca cggaaatttt ggcccggcag
     3181 cacgccgtca cgctggcgga ccggctggag cccttcggcg tcaatgtcac gctgctggtc
     3241 ggcggcatga aggccaaaga aaaacgcatc gcgctggaag ctatcgccga cggccgggcc
     3301 aatttggtcg taggtacgca tgccgtgctg acggacaccg tggaattcaa aaatctcggt
     3361 cttgccatcg tggacgagca gcaccgtttc ggcgtgcggc agcgcggcct gttggcgggc
     3421 aaggcccaaa gcccgcacct gctggtcatg agcgccacac ccatcccgcg cacgctgggg
     3481 cttttgatgt acggcgacct ggatatttcc gtcctggacg agctgccgcc aggccgcaag
     3541 cccatcaaaa catggtttat caccggcaaa aagcgccggg atatgtacgg ctttttggac
     3601 aaacaaatcg cggcggggca tcaggtgtac atcgtctgcc cggccatcga ggaaaatgag
     3661 gccatgggcg acctgcaggc cgtgacgacc tactacaccg agacggcctg cgcgc
//
LOCUS       DBMDSQ010000203         3695 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_5740_length_3695_cov_1.527824, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000203 DBMDSQ010000000
VERSION     DBMDSQ010000203.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 3695)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3695)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3695
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5740_length_3695_cov_1.527824"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..927
                     /locus_tag="ACZPAX_07545"
     CDS             <1..927
                     /locus_tag="ACZPAX_07545"
                     /inference="COORDINATES: protein motif:HMM:NF013700.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HIR1113792.1"
                     /translation="FEENGTYDAKLVVKVDGSSSDRDSSVTGTETWQFQFTVNIKPTM
                     QLCTPSVQQGSVAAVRVGPTLSGEAPAIKTALQTPGFVQAANGWICYLPIPWNESTGN
                     VTLTVTADGYTEDMTLSIRAASYTYKDYSKTSQMISPYIGESDAPAAVTKVLSTTDDS
                     IEWAVGGFVQPFLDSFDTPLIYGMTEYAGRARSERSTNYGYGGRTATNVVIKPKKSND
                     SMIVPASGRVLLAEDLGGIYGNTVVIEHGAGLKSIFYGLGALNVKAGENVKQGQLLGV
                     CSKTVVAEMRIGTVPINPLLVWRGQCDGLKNY"
     gene            1072..1875
                     /locus_tag="ACZPAX_07550"
     CDS             1072..1875
                     /locus_tag="ACZPAX_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR1113793.1"
                     /translation="MPKTFCVVGHDARQQAAARVLRRAGYGVTAADNAAAADYILLPM
                     SQGRVSDEVARALQGAGQGTWIFAGRPGMPVRTAVRQANLPLVDYFLRPELESLNAVP
                     TAEGCLELLLRLRQRTIWESDFLVLGYGRVGRAVARRLGLLGGRVTVAARSAEQRANA
                     RCAGCRAAPLTALPGLLPEFDAVINTIPAPVLPRALLQKLPGGALIIDLASLPGGTDF
                     AAAEELGLHAEHALALPGRCAPQTAGALIAQTVLTILEERGEYAERSTP"
     gene            1872..2453
                     /locus_tag="ACZPAX_07555"
     CDS             1872..2453
                     /locus_tag="ACZPAX_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005943861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit B"
                     /protein_id="HIR1113794.1"
                     /translation="MTTQPKRTVAFALCGSFCSFSAVLPQVRLLAARGWDVLPILSAS
                     AAEQDTRFGTAAALQQTLYEVTGHKPLTTLQGVEPLGPQKLADALVIAPATGTTMALL
                     AAGISSTPVTLAAKSLLRGGRPIVIAPSTNDGLSGSAPALGQLLQRRHYYFVPFGQDD
                     SYKKPCSLKSDFTLLPDTLEAALRGVQIQPMLL"
     gene            2511..>3695
                     /locus_tag="ACZPAX_07560"
     CDS             2511..>3695
                     /locus_tag="ACZPAX_07560"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF006873.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HIR1113795.1"
                     /translation="MQTITIHGRQPLHGSIRPAAAKNSALPLLAASLLCGGPCRLRGV
                     PALADVDTSLALLQAVGADAQRCGADICTRPADALCGEIPDYLAGAMRSSVFYLAPLL
                     CRAGYVRLPLPGGCRLGPRPVDIHLAGLASMGAEVELEGIAVTLRRKGPLHGVDFTLR
                     LPSVGATMTLLMAACCAQGRTVLRGAAREPEIGDMIAFLCACGADVSGYGTSILTVQG
                     GRPLSGAVHAPLPDRIAAATYAAALACAGGEVTVTHCAPAYYAAFLDFLQAAGVDVAR
                     QDTTVTLARSPARPLRGGQTLCANAWPAFATDTAPLAAAVLLTADAPSEIYDHLFARR
                     FACAEGFAALGARCRTTGRTLYMDGRAALHGAAVTAPDLRGGAALLLAALGAEGVTFL
                     RDP"
BASE COUNT          596 a         1278 c         1186 g          635 t
ORIGIN      
        1 tttgaagaaa acggcaccta cgacgccaag ctcgtcgtca aggtcgatgg aagctcgtcc
       61 gaccgcgatt ccagtgtcac cggtaccgag acctggcagt tccagttcac ggtcaacatc
      121 aagcccacca tgcagctctg cacgccgtcc gtgcagcagg gcagcgtggc ggcggtgcgc
      181 gtcggcccga cgctgagcgg cgaagccccc gccatcaaaa cggccttgca gacccccggc
      241 tttgtccagg cggccaacgg ctggatctgc tatctgccca ttccctggaa cgagagcacc
      301 ggcaacgtga ccctgaccgt caccgccgac ggctacaccg aggatatgac gctgtccatc
      361 cgcgcggcgt cctacaccta taaggattac agcaaaacgt cccagatgat ctcgccctac
      421 atcggcgaga gcgacgcccc cgccgccgtc accaaggtgc tttccaccac ggatgacagc
      481 atcgagtggg ccgtgggcgg ttttgtacag ccgttcctgg attccttcga tacaccgctg
      541 atttacggca tgaccgagta cgcaggccgc gcccgcagcg aacgcagcac caactacggc
      601 tacggcggcc gcaccgcgac caatgtcgtc atcaagccga agaagtccaa cgattcgatg
      661 atcgtgcccg ccagcggccg cgtgctgctg gccgaggacc tgggcggcat ctacggcaat
      721 accgtcgtta tcgagcacgg cgccggcctc aagagcattt tctacggcct gggtgcgctg
      781 aacgtcaagg ctggcgagaa cgtcaagcaa ggccagcttt tgggcgtttg cagtaaaacc
      841 gtcgtcgccg aaatgcgcat cggcaccgta cccatcaacc cgctgcttgt ctggcgcggg
      901 caatgcgatg ggttgaagaa ttattgatat tgtataacgg cacccgctga aaacggcggg
      961 tgctgttttt ttgttgtaca aggcttcccc ccgaggaggg aaggcttagg caattcacac
     1021 ctcccgccgc cccggcatac tatatcccaa aaccaaccga ggtgagcgct tatgccgaaa
     1081 acattttgcg tagtggggca cgatgcccgc cagcaggcgg cggcccgtgt gctgcgccgt
     1141 gcaggctacg gcgtgaccgc agccgacaat gccgccgcag ccgattacat cttattgccc
     1201 atgtcccagg gccgcgtcag cgacgaagtt gcccgcgcct tgcagggggc cgggcagggc
     1261 acatggattt ttgcgggccg tcccggtatg ccggtgcgca ccgccgtgcg gcaggccaat
     1321 ctgccgctgg tggattactt cctgcggccc gagctggaat ctctgaacgc cgtgcccacc
     1381 gccgaaggct gcctggaact gctgctgcgc ctgcgtcagc gcacgatctg ggagagtgac
     1441 tttcttgtgc tgggctatgg ccgggtaggc cgcgccgtgg cccggcggct gggacttctg
     1501 ggcgggcgcg tcactgtggc tgcccgcagt gccgagcagc gcgccaacgc ccggtgtgcg
     1561 ggctgccgcg ccgcgccgct gaccgccctg cccgggctgc tgccggagtt tgacgccgtc
     1621 atcaacacga tccccgcgcc ggtgctgccc cgggcactgc tgcaaaaact gccgggcggg
     1681 gcgctgatca tcgatcttgc cagcctgccg ggcggcaccg attttgccgc cgccgaggaa
     1741 ctgggtctgc acgctgagca cgccctggcc ctgccgggcc gctgtgcgcc ccaaacagcc
     1801 ggggcactga ttgcgcagac cgttctgaca attttagagg aacgcggcga atacgcggaa
     1861 aggagcaccc catgaccacc caaccgaaac gcacggttgc ctttgcgctg tgcggcagct
     1921 tttgcagctt ttccgccgtg ctgccccagg tgcggctgct cgccgcgcgg gggtgggatg
     1981 tcctacccat tttaagcgcc agcgctgccg agcaggacac ccgcttcggc acggccgccg
     2041 ccctgcagca gaccttgtac gaagtcaccg gccacaaacc gctgaccacc ttgcagggcg
     2101 tcgagccgct gggcccgcag aaattggccg acgccctcgt cattgccccg gccaccggca
     2161 caacgatggc cctgctggcg gcagggatca gttcgacccc ggtcacactg gccgccaaga
     2221 gccttttgcg cggcgggcgg cccatcgtca tcgcaccgtc cacgaacgat gggctttcgg
     2281 gcagtgctcc ggcgctgggc cagcttttgc agcggcggca ctactatttt gtgcccttcg
     2341 gccaggacga cagctacaaa aagccgtgca gcttaaagag cgattttacc cttctgcccg
     2401 acaccctgga agccgccctg cgcggcgtgc agatacaacc gatgctgttg taatatgtgg
     2461 ccgggcaggc ggcatataac aaggtagaac acattcaggg aggcccgctc atgcagacga
     2521 tcacgattca cggcaggcag cccctgcacg gcagcatccg cccggcggcg gccaaaaaca
     2581 gcgccctgcc gctgctggcg gcgtcactgc tctgcggcgg gccgtgccgt ctgcgcggcg
     2641 tgcccgccct ggccgatgtt gacaccagcc tggccctttt gcaggctgtg ggggccgacg
     2701 cgcagcgctg cggcgcggat atctgcaccc gccccgccga tgctctctgc ggcgagattc
     2761 ccgactacct ggccggggcc atgcgcagct cggtgttcta tctggcgccg ctgctctgcc
     2821 gggcggggta tgtgcggctg cccctgccgg gcggctgccg cctggggccg cgtcctgtgg
     2881 atatccacct ggccgggttg gcctccatgg gggccgaggt agaattagag ggcatcgccg
     2941 tgacgctgcg gcgcaaaggg ccgctgcatg gggtggattt cacactgcgg ctgcccagcg
     3001 tcggtgctac aatgacactg ctgatggccg cctgctgcgc ccagggccgt actgtgctgc
     3061 gaggcgcggc gcgggagccg gaaatcggcg atatgattgc ctttctgtgc gcctgcgggg
     3121 ctgacgtttc cggctacggc acgtcgattt tgacggtgca gggcgggcgg cccctgagcg
     3181 gggcggtgca cgccccgctg cccgaccgca ttgccgccgc cacctacgcc gccgccctgg
     3241 cctgcgccgg gggagaggtg accgtcaccc actgcgcccc cgcctattat gcggcctttc
     3301 tcgacttttt acaggcggcg ggcgtggatg ttgcccgcca ggataccacc gtcacgctgg
     3361 cccgaagccc cgcccggcct ttgcgcggcg ggcaaacgct ttgcgccaac gcctggcccg
     3421 cctttgccac cgataccgcg cccctggctg ccgccgtgct gctgaccgcc gacgccccca
     3481 gcgagatcta cgaccacctg tttgcccgcc gctttgcctg cgccgagggc tttgccgccc
     3541 tgggggcaag atgccgcacc accgggcgta cactttatat ggacggccgc gcagccctgc
     3601 acggagccgc ggtgactgcc cctgacctgc gcggcggggc cgccctgctg ctggctgcac
     3661 tgggggccga aggcgtgacc ttcctgcggg acccc
//
LOCUS       DBMDSQ010000204         3685 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_5762_length_3685_cov_1.343471, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000204 DBMDSQ010000000
VERSION     DBMDSQ010000204.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 3685)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3685)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3685
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5762_length_3685_cov_1.343471"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..753
                     /locus_tag="ACZPAX_07565"
     CDS             <1..753
                     /locus_tag="ACZPAX_07565"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR1113796.1"
                     /translation="VAQAVSDGLYKRCDRLIAMSEPLQERLCVRTGKTAAEVAIIPQY
                     CEDFYAVPQPDPVLEERFAGRFNLVFTGTFTPAQSLDMVINAVLDARAAGAEALHLLL
                     VGDGMSREALQKQVADLCAEDAVTFYGSVPATDVPKFTALADALLISLSDSPDLGLTV
                     PGKLASYMAAAKPVLASMNGAGYAAVKESGGGLVSPACDRRALADNMLALYRMSNDER
                     AALGQKAYAYYAAHYRRAELLKRLEEFTVEGK"
     gene            833..>1177
                     /locus_tag="ACZPAX_07570"
     CDS             833..>1177
                     /locus_tag="ACZPAX_07570"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR1113797.1"
                     /translation="MDKKILVIIPCYNEEDNIVNTVENLRAVCPEVDYLVVNDCSTDR
                     SAEILKEHHYPFLDLPVNLGIGGNVQCGYLYAVHHGYDVAVQMDGDGQHDPAYLMEIV
                     QPVLNGECDMCIG"
     assembly_gap    1178..1187
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1188..1559
                     /locus_tag="ACZPAX_07575"
     CDS             <1188..1559
                     /locus_tag="ACZPAX_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR1113798.1"
                     /translation="RFIKKEGFQTSFMRRVGIRWLSGMIYLLCGKRVLDVTSGFRATN
                     AGMTAFFSRHYAADYPEPEAILAASLAGYHVGEAPVIMRERQGGVSSIHSFKSAYYMI
                     KVTLALIIDRLSIRKEKGGCA"
     gene            1556..1915
                     /locus_tag="ACZPAX_07580"
     CDS             1556..1915
                     /locus_tag="ACZPAX_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2304 domain-containing protein"
                     /protein_id="HIR1113799.1"
                     /translation="MTAHLQLFMILGAVVLLLIIFALLKRGLMSVKYSLLWLLLAVGL
                     VIAAAFPYVVYVLRDLLDIEMPVNLVFLLMFCFVLVVLLSLSIAISQLADKCKRLTQA
                     NAILEKRVRDLEDTLKK"
     gene            1943..3445
                     /locus_tag="ACZPAX_07585"
     CDS             1943..3445
                     /locus_tag="ACZPAX_07585"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HIR1113800.1"
                     /translation="MTITWEQILAFLCEDQSMAITSLLFLAFFAAVALINYALPRVLR
                     PYFLLIASYGFFCYDPVNRPLVWVIAAATVVTWLCGLIIGKVKNKAVRVLALLVSVGF
                     GIGILIYYKYWNLLADSLGGSFLSHRENLLAPLGLSYFTFAAMSYTIDVFKRRCRVET
                     NPLHYALFVSFFPTLINGPIERYPQMRPQIQKSRRFSYGRCAGGAFRMLWGYTKKMVI
                     ADNLSQYVSLVYGDVSGISGPNLVAATVLFAVQLYMDFSGCCDIALGAARILGYDLIE
                     NFQSPFEARTFNDFWRRWHISMTSWFRDYVYFSLGGSRCAPWRHYLNIVIVFVCSGLW
                     HGADWRYLAWGLFTGILAAFGVMTAKLRRRINRWNPLYRMGWFKALWQTIVTDGLFCL
                     TLVFFASAIYNTDPFAVYGSLLQGWDGLSGSWAQVSNLIYSSGIDGRLPVALLFGCFV
                     VFAAEHGGRSVAKWIRQQVWPLRWTLYYGAAAAILFFAAFGQSAFIYQQY"
     gene            3464..>3685
                     /locus_tag="ACZPAX_07590"
     CDS             3464..>3685
                     /locus_tag="ACZPAX_07590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113801.1"
                     /translation="MPKSPTPIPAPPVKIQRGAPSRAPAKKRRRKKKRNPFLWFLQGL
                     VRRIYFGLKTGFKFLLLVPVLVFMVAFSGF"
BASE COUNT          667 a         1094 c         1111 g          803 t
ORIGIN      
        1 gtggcccagg cggtcagcga cggcctgtac aagcggtgcg accggctgat tgccatgagc
       61 gagccgctgc aagaacggct gtgcgtccgc accgggaaaa cggcggcgga agtggcgatc
      121 atcccgcagt actgcgagga tttctacgcc gtgccgcagc ctgatccagt gctggaagaa
      181 cggtttgccg ggcggttcaa cctggtattc accggcacct tcacccctgc gcagagcctg
      241 gacatggtca ttaacgccgt gctggacgcc cgcgccgccg gggcggaagc actgcacctg
      301 ctgctggtgg gcgacggcat gagccgcgag gccctgcaaa agcaggtggc tgatttgtgc
      361 gccgaggacg ccgtgacatt ctacggcagc gtgcctgcca ccgatgtgcc gaagttcacg
      421 gcgctggcgg acgcgctgct gatttccctg tccgactcgc ccgatcttgg gctgaccgtg
      481 cccggcaagc tggccagcta tatggccgcc gccaagccgg tgctggcctc aatgaacggc
      541 gcagggtatg cggcagtaaa agagagcggc ggcgggctgg tcagcccggc ctgtgaccgc
      601 agggcgctgg cggataatat gctggcgctg taccgcatga gtaacgacga gcgggcggcg
      661 ctggggcaga aagcctacgc gtattacgcc gcgcactacc gccgggccga gctgctgaag
      721 cggttggagg aattcacggt ggaaggaaaa tgaggaagcc ctcgcagcct tgccgttacc
      781 gcctctatcc cacaaaaacg caacacaccc aaaattgaga ggtacaccca ttttggataa
      841 aaaaattctt gtcattatcc cctgctataa tgaggaagac aacatcgtca acacggtgga
      901 aaatctgcgg gcggtctgcc cggaggtgga ctatcttgtg gtcaacgact gctccaccga
      961 ccgcagtgcg gaaattttga aagagcacca ctatcccttc ctcgacctgc cggtcaactt
     1021 agggataggt ggcaatgtgc agtgcggcta cctgtatgcc gtacatcatg gctatgatgt
     1081 cgccgtgcag atggacggcg acggccagca cgaccccgcc tacctaatgg agattgtgca
     1141 gccggtgctg aacggcgaat gcgatatgtg catcggcnnn nnnnnnncgt tttattaaaa
     1201 aggaaggatt ccagaccagc tttatgcgcc gggtcggcat ccgctggctg agtgggatga
     1261 tctatctgct gtgcggcaag cgggtactgg atgtgaccag cgggttccgc gccaccaacg
     1321 ccggcatgac cgcgtttttc tcccggcatt acgctgccga ttaccccgag ccggaagcca
     1381 ttctggctgc cagcctggcg ggctaccatg tgggcgaggc cccggtcatc atgcgggagc
     1441 gacagggcgg cgtgtccagc atccacagct tcaagagtgc ctactacatg atcaaagtca
     1501 cgctggcgct catcattgac cgactgtcca tccgcaagga gaaagggggc tgcgcatgac
     1561 cgcacacttg cagctgttta tgattctggg cgcagtggtg ctgctgctca tcatctttgc
     1621 ccttttaaag cgcgggctga tgagcgtaaa gtacagcctg ctgtggctgc tgctggccgt
     1681 ggggctggta attgccgcgg cgtttcccta tgtggtgtat gttctgcggg acctgctgga
     1741 cattgaaatg ccggtcaacc tggtattttt gctgatgttc tgctttgtac tggtggtact
     1801 gctgtccttg agcattgcca tcagccagct ggccgacaag tgcaagcggc tgacccaggc
     1861 gaacgcgatt ctggaaaagc gtgtccgcga cctggaagat accctgaaga aataaccctg
     1921 cggcgaaacg gagagaggaa tcatgacaat aacctgggag caaatcctcg cctttttgtg
     1981 cgaggatcag tcaatggcga tcacctcgct gttgtttctt gccttttttg cggcggtggc
     2041 actcatcaac tatgccctgc cccgcgtgct gcggccgtat tttctgctga tcgccagcta
     2101 cggcttcttc tgctatgacc cggtcaaccg cccgctggtc tgggtcattg ccgccgctac
     2161 ggtcgttacc tggctgtgcg ggcttatcat cggcaaggtc aaaaacaagg ccgtgcgggt
     2221 gctggcccta ctggtctcgg tagggttcgg catcggtatt ttgatttact acaaatactg
     2281 gaacctgctg gcggattctc tgggcggcag cttcctctcc caccgggaga atctgctggc
     2341 ccccctgggc ctcagctact tcacctttgc ggccatgagc tacaccatcg atgtgttcaa
     2401 gcgccgttgc cgggtcgaaa cgaacccgct gcactatgcg ctgttcgtca gcttcttccc
     2461 cacgctcatc aacggcccca tcgagcgtta cccccagatg cggccgcaga ttcaaaagag
     2521 ccgccggttc agctatggcc gctgcgcagg cggcgcattc cgtatgctgt ggggctacac
     2581 aaaaaaaatg gtcatcgccg acaacctgag ccagtatgtc tcgctggtct acggtgatgt
     2641 ttccggcata agcggcccga atctcgttgc cgccaccgtg ctgttcgccg tgcagctgta
     2701 catggatttc tccggctgct gtgatatcgc cctgggcgcg gcgcgtatcc tggggtacga
     2761 cctgatcgaa aacttccagt ccccctttga ggcccgcacc ttcaacgatt tctggcggcg
     2821 gtggcatatc tcgatgacca gctggttccg ggattacgtc tacttcagcc tgggcggcag
     2881 ccggtgcgca ccatggcggc actacttaaa catcgtcatc gtctttgtct gctccggcct
     2941 gtggcacggc gcggactggc gctacttggc ctggggcctg ttcaccggca ttctggcggc
     3001 cttcggcgtc atgacggcca aactgcgccg ccgcatcaat cgctggaatc cgctctaccg
     3061 catgggctgg ttcaaggcac tgtggcagac gattgtcacg gacgggctgt tttgcctgac
     3121 gctggtgttc tttgcatcgg ccatctataa taccgacccc ttcgccgtct acggttcgct
     3181 cctgcagggc tgggatggcc tgtctggctc ctgggcacag gtgtctaacc tgatttacag
     3241 cagcggcatt gatggccgtc tgccggtggc gctgttgttc ggctgctttg tcgtttttgc
     3301 ggcagagcac ggtggccgca gcgtcgccaa atggattcgc cagcaggtct ggccgctgcg
     3361 ctggacgctc tactacggcg cggctgcggc gatcttgttc ttcgccgcgt tcggtcagtc
     3421 ggcgttcatc taccagcaat actaaggaaa ggcggtgtat gttatgccca agtcccctac
     3481 cccgatcccc gcaccgccgg tcaaaatcca gcgcggtgcc cccagtcgcg ccccggccaa
     3541 aaagcgccgc cgcaagaaaa agcgcaaccc cttcctctgg tttttgcagg ggcttgtccg
     3601 ccgcatctac tttggcctga aaaccgggtt caagttcctg ctgttggtgc cggtgcttgt
     3661 gtttatggtg gcgttcagcg ggttc
//
LOCUS       DBMDSQ010000205         3663 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_5808_length_3663_cov_1.643459, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000205 DBMDSQ010000000
VERSION     DBMDSQ010000205.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 3663)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3663)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3663
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5808_length_3663_cov_1.643459"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..668)
                     /locus_tag="ACZPAX_07595"
     CDS             complement(<1..668)
                     /locus_tag="ACZPAX_07595"
                     /inference="COORDINATES: protein motif:HMM:NF019887.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HIR1113802.1"
                     /translation="MKPSEVNPMKYKLLLLDIDGTLRPGSCERVPRENAEAVCAVQKA
                     GVKIAIATGRGRIGVGKGLLRSIRPDYWVCAGGAQLVDYKGADLALHRLTTEEMYALV
                     DFFENYDLPLRFTFHDANHAYIGYEEFARREREKNLALNIVDGEDQDHHLTEMPFGAF
                     GFLPQAMAEKFQEQYGYLGLNFLFSYPGSDGCDILQKGIDKGTGLLELAKLAGLDPAD
                     CVAVG"
     gene            761..1429
                     /locus_tag="ACZPAX_07600"
     CDS             761..1429
                     /locus_tag="ACZPAX_07600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005618 - cell wall [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MreC domain-containing protein"
                     /protein_id="HIR1113803.1"
                     /translation="MQLDLSRSRRRRRWWLLPLVLAAVLLAGPWLAAVVPGTAASIDR
                     FVGARYTAQIDALQTENFTLHQQLARSADALAENDALRRLAGCGRVSAAVAPARVLAR
                     GVSGFTLACPGAVSGAAVLDAGGRYAGIVTAVDGDTCTVALVRTAGLCGAYAGLVTPG
                     IWQLTGLPADCGLAAGDVVTTPSGDWLGTLAAAPMPDADGLTASATLADTADLCASVY
                     FVKK"
     gene            1521..3311
                     /locus_tag="ACZPAX_07605"
     CDS             1521..3311
                     /locus_tag="ACZPAX_07605"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF013100.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HIR1113804.1"
                     /translation="MSNRKNAHRRRLRVAVLAVVCGAVFAAFFARLAWMQFARADYYA
                     DKAAEASSASYTITQHAARGVITDANGVVLARDTTVYDLYLRIPAPPGTDLRETVKNI
                     ETLTGSKDVETQLAAFCSAASAGELAVARGADADMLTAFYTAGLVQSGAVRPAARGVR
                     SWPGGTLLPHALGLTGPITAAQWPAAKARGLAMDAVIGQSGLEAAYDDFLRGQDGRVL
                     INTGFDGAVRRTAQLRQAVPGAALVLTVDSSLQKALQDALISQIHTLQATKAAGAGRE
                     CRAGAAVVVDVKTGGILAAASVPGFDLNACRADYAALAADAAAPLLDRTAQGLYAPGS
                     AFKPAVAAAALTAGIDPAATVNCTGRYGFYSGYQPGCLQYGHSGPVDLRTALEHSCNI
                     FFYDVGRRLGVDTFSAMAQQLGLAAPTGVEVAEARGRLTWSSDGNYQAGLTLMAAIGQ
                     GNTAVTPLQLAAYAATLANYGQRPALHYADRAVSAATGETLWQYTPTFTAVQGGEAVF
                     GPIRDGMKRMAQTTRVLREAPVVCAAKTGSPQLADTLPGGSHYVNSVLIGYAPADDPQ
                     IAVAVVLEYGGGGANAAPILRAVLDAVFGI"
     gene            3526..>3663
                     /locus_tag="ACZPAX_07610"
     CDS             3526..>3663
                     /locus_tag="ACZPAX_07610"
                     /inference="COORDINATES: protein motif:HMM:NF020704.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide-binding protein"
                     /protein_id="HIR1113805.1"
                     /translation="MVCSGKGGTGKSTVSVLLGACLARLGRKVLLIELDSGLRSVDII
                     AG"
BASE COUNT          601 a         1183 c         1184 g          695 t
ORIGIN      
        1 cccacggcca cacagtccgc cgggtccagc cctgcgagct tggccagctc caacaggccg
       61 gtacccttgt cgatgccctt ttgcaggata tcgcagccgt cgctgccggg gtaggagaag
      121 aggaagttca gcccgaggta gccgtactgt tcctggaatt tttcggccat cgcctgcggc
      181 aaaaagccga acgcgccgaa gggcatttct gtcaggtgat ggtcctgatc ctctccgtcc
      241 acgatgttca gcgcaaggtt tttctctcgc tcacgccggg cgaactcctc gtagccaata
      301 taggcgtggt tcgcatcgtg gaaggtgaac cgcagcggca ggtcgtaatt ttcaaaaaag
      361 tccaccaggg cgtacatttc ctcggtcgtc agacggtgca aggccagatc cgcacccttg
      421 tagtccacca gctgggcacc gcccgcacag acccagtagt ccggccgaat gctgcgcagc
      481 agacccttgc ccacaccgat gcgcccgcgc ccggtggcaa tagcgatttt aacgcccgcc
      541 ttctgcacag cgcagacggc ctcggcattt tccctgggta cacgctcaca gctgcccggc
      601 cgcagcgtac cgtcgatatc gagtaataat agtttatatt tcatagggtt gacctcactt
      661 ggtttcatgg aatcgctccc acatcctttt atcattgttt tctattatac caaagtctaa
      721 ccccctcccg caaggcatat tctgctttgg ggtgatgttt gtgcagctgg atctttcgcg
      781 ttcccgcagg cgtcggcggt ggtggcttct gccgttggtg ttggccgccg ttttgctggc
      841 tgggccgtgg ctggccgctg ttgtgccggg cacggctgct tctatcgacc gattcgtcgg
      901 ggcgcggtat acggcgcaga tcgacgcgct gcaaaccgaa aatttcacgc tgcaccagca
      961 gcttgcccgc agtgccgatg ccctggccga gaacgatgcc ctgcgccggc tggccgggtg
     1021 cgggcgggtc agcgccgctg tggccccggc gcgggtcctt gcccggggcg tgagcggctt
     1081 cacgctggcc tgtccggggg ccgtgtccgg ggccgccgtg ctggacgcag gcgggcggta
     1141 tgcgggcatc gtcaccgccg tggacggcga cacctgcacc gtagcgctgg tccggactgc
     1201 cgggttgtgt ggggcttatg ccgggctggt cacaccgggg atatggcagc tgaccggtct
     1261 gcccgccgac tgcgggttag cggcggggga cgtcgtaaca acgccgagcg gcgactggct
     1321 gggcacgctg gccgccgcac ctatgcccga cgccgacggc ctgaccgcca gcgctaccct
     1381 ggccgatacc gccgatttgt gcgccagcgt ctatttcgtg aaaaagtaat tgctttttcg
     1441 tcgaaaatta cgtacaataa atatgtatgt tgcgtgggct tatgccccat accttcatct
     1501 atatttttcg aggtgattcc atgtccaacc gcaagaatgc ccaccgccgc cgtctgcgcg
     1561 tggcggtgct ggccgtcgtg tgcggcgcgg tgtttgcggc gttctttgcc cggctggctt
     1621 ggatgcagtt tgcccgcgcc gactactacg ccgacaaagc cgccgaggcc agttcggcca
     1681 gctacaccat aacccagcac gccgcacggg gcgtcatcac cgacgcaaac ggcgtggtgc
     1741 tggcccggga caccaccgtg tacgatttat acctgcgcat cccggccccg ccggggaccg
     1801 acctgcgcga gactgtaaaa aacatagaga ccctgacagg cagcaaggat gtggaaacac
     1861 agcttgctgc cttttgttct gccgcctcgg cgggggagct ggccgtggcc cggggcgcgg
     1921 atgctgatat gctgaccgcc ttctacacgg cgggccttgt gcagagcggc gcggtgcggc
     1981 ccgccgcccg gggcgtgcgc agctggccgg gcggtacgct gctgccccac gcgctgggcc
     2041 tgaccggccc tatcaccgcc gcgcaatggc ccgccgccaa agcgcggggc ctggccatgg
     2101 atgccgtcat cggccagagc gggctggaag ccgcctacga cgactttctg cgcgggcagg
     2161 atgggcgggt gctcatcaac accgggtttg acggggccgt gcgccgtacc gcgcagctgc
     2221 ggcaggccgt acccggggcc gcgctggtgc tgacggtgga cagttccctg caaaaagcac
     2281 tgcaggacgc actgatttcg cagatccaca ccctacaggc caccaaggcc gccggagccg
     2341 ggcgggagtg ccgcgccggg gccgccgtgg tcgtagacgt gaaaaccggc ggcatcctgg
     2401 ccgccgccag cgtgccgggg tttgacctga acgcctgccg tgcggactac gcggcccttg
     2461 ccgccgatgc cgccgcgccg ctgctggacc gcaccgccca ggggctgtat gcgccgggtt
     2521 cggctttcaa gcccgccgtg gcagccgccg cgctgactgc aggcattgac cctgccgcca
     2581 ccgtgaattg caccgggcgg tacggctttt acagcggcta ccagcccggc tgcctgcaat
     2641 acggccacag cggcccggtc gacctgcgca ccgcgctgga acacagctgc aatatctttt
     2701 tttacgatgt cgggcggcgg ctgggggtag atactttctc cgccatggcg cagcagctgg
     2761 gcctggccgc ccccaccggt gttgaggtcg ccgaagcccg gggccgtctg acctggtcca
     2821 gcgacggaaa ctaccaggcc gggcttacgc tgatggcagc catcgggcag gggaacactg
     2881 ccgtgacgcc gctgcagctg gccgcctacg ccgccacgct ggccaactac ggccagcgcc
     2941 ccgcactgca ctacgccgac cgcgccgtga gcgccgccac cggcgagacg ctgtggcagt
     3001 acacaccgac cttcaccgcc gtgcagggcg gcgaggccgt gtttggcccc atccgggacg
     3061 gcatgaagcg gatggcccag acgacccgcg tgctgcggga ggccccggtc gtctgcgccg
     3121 caaaaacggg cagtccgcag ctggctgaca ctttacctgg cggcagccac tatgtcaact
     3181 cggtgctcat cgggtacgcg cccgccgacg acccgcagat cgccgtggcc gtcgtgctgg
     3241 aatacggcgg gggcggggcc aacgctgcgc ccatcctgcg ggcggtgctg gacgccgtgt
     3301 tcgggatatg aaacgtttcc attttttcgc agtccatcgc tattccttgt gcattttgtc
     3361 catatttgtg catcaaaaag tataccttat cgtgattttc ttcaaaaaga aggtgtcctt
     3421 ttctgacaaa aagccagttg cagttcatcc ggaaatgtgc tagaatatac gggagatagt
     3481 gttgcacaac tgcggaaggg agccgacatg aacgcacagt gtatcatggt ctgctccggc
     3541 aagggcggca ccggcaaaag cacggtttcg gtgctgctgg gggcctgttt ggcgcggctg
     3601 gggcggaagg tcctgctcat tgagctggat tcgggtctgc gtagcgtgga cattattgca
     3661 ggc
//
LOCUS       DBMDSQ010000206         3660 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_5812_length_3660_cov_1.538353, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000206 DBMDSQ010000000
VERSION     DBMDSQ010000206.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 3660)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3660)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3660
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5812_length_3660_cov_1.538353"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..206
                     /locus_tag="ACZPAX_07615"
     CDS             <1..206
                     /locus_tag="ACZPAX_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1113806.1"
                     /translation="IPGTTVFIIAQRISSVENADKVLVLDNGRVSGFDTPANLLKNNA
                     IYQDVYNSQTKGSGDFDEKGGEA"
     gene            206..2137
                     /locus_tag="ACZPAX_07620"
     CDS             206..2137
                     /locus_tag="ACZPAX_07620"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1113807.1"
                     /translation="MAQQAKVVKVGPGGRNNGPRPKVENPGKVFKRILAYVMSRYKAQ
                     VIVVLCCILLAVFAQLQGVMFSQTLIDGYILPLLNSGSTDFSGLAAAIARVAAFYCVG
                     ICAVFVQNRLMAKITQGTLKDLRDEMFTHMQTLPIKYFDTHAHGDIMSVYTNDIDTLR
                     QMISQSLPQLVNTIVTIIGVLFSMIYLSIPLTLLALAMVGVMLLATKYLTGNSGKFFI
                     KQQQELGKVNGYIEEMMNGQKVVKVFCHEEAAIKEFNELNDELFHSADKANAYSLVAM
                     PVNGQLGNLSYVLCAIIGGALVISGFGGSLSLGGLASFLVLTRQFNQPISQISMQLNS
                     VVMALAGGARIFALLDEKPEVNEGDITLVHAKYAADDTVTETNESTGMWAWKHIGADG
                     KPQYTELKGDIVFKDVDFGYDEKKIVLHDINLYGRPGQKIAFVGSTGAGKTTITNLIN
                     RFYDIQKGQILYDGHDIKSIEKDALRSSLGIVLQDTHLFTGTVMENIRYGRLTATDEE
                     CMAAAKLANADTFIKHLPDGYNTMLTGDGTNLSQGQRQLLAIARAAVADPPVLILDEA
                     TSSIDTRTEKLVQDGMDGLMYGRTTFVIAHRLSTVRNSDCIMVLEQGRIIERGTHDEL
                     IAQKGRYYRLYTGNFAENS"
     gene            2378..3214
                     /locus_tag="ACZPAX_07625"
     CDS             2378..3214
                     /locus_tag="ACZPAX_07625"
                     /inference="COORDINATES: protein motif:HMM:NF012859.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RICIN domain-containing protein"
                     /protein_id="HIR1113808.1"
                     /translation="MAPKKKIAQTVITEGKFYTISAANGKVVEVADYNIDNGAKIQLW
                     DNANAEWQQWNFVAAGDGVYRIQNRFTAKMLDLDMGGVSDGTRVHQWEGAQASSQLWV
                     VEPTNDGRVKIKSNLAGKLLDPGMATENGTVLQIWADVNGDNQFWTINEVTRKPKTSV
                     KASAVKAKAAAEKAATEAVKAAEPVVEKAVKAAKPAVEKAVKAAEPVVEKAVETAKPV
                     VEETVKAAKPVVEKAVKAAEPVVEKAVEAAKPVVEKTVEAAKTAVAKTNTRKTTKGKR
                     RK"
     gene            complement(3469..>3660)
                     /locus_tag="ACZPAX_07630"
     CDS             complement(3469..>3660)
                     /locus_tag="ACZPAX_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015565625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113809.1"
                     /translation="KELARLNKELTKAEKELGMFTNQLNNPKFVEKAPAKLVEETRAK
                     LAAAQEKAAKIKESIAALG"
BASE COUNT          824 a         1100 c         1049 g          687 t
ORIGIN      
        1 agatccccgg cacgacggtc ttcatcattg cccagcgcat ttcctctgtg gagaatgcgg
       61 acaaggtgct tgtgctggat aacggcaggg tcagcggttt tgacacaccc gccaacctgc
      121 tgaaaaacaa cgcgatttac caggatgtct acaacagcca gaccaagggc tccggcgatt
      181 ttgacgagaa gggaggggag gcataatggc acagcaggca aaagttgtca aggtcggccc
      241 cggcgggcgc aataacggcc cccgccccaa ggtcgaaaac cccggcaagg tgttcaagcg
      301 catccttgcc tatgtgatga gccgctataa ggcacaggtc attgtggtgc tctgctgcat
      361 cctgctggcg gtgttcgccc agctgcaggg cgttatgttc tcccaaacgc tgattgacgg
      421 gtacatcctg ccgctgctga attccggcag cacggatttc agcggcctgg ccgccgccat
      481 cgcccgcgtg gcagcgttct actgcgtcgg catctgcgcc gtatttgtgc agaaccgcct
      541 gatggcaaaa atcacccagg gcacgctgaa agacctgcgt gacgaaatgt tcacccatat
      601 gcagacgctg cccatcaaat actttgacac ccacgcccac ggcgatatca tgtcggtcta
      661 caccaacgat atcgacaccc tgcgccagat gatcagccaa agtctgccgc agctcgtcaa
      721 caccatcgtc accatcatcg gcgtgctgtt ctcgatgatt tacctcagca tccccctgac
      781 gctgctggcg ctggctatgg tcggcgttat gctgttggca accaagtacc tgaccggaaa
      841 ctccggcaag ttcttcatca agcagcagca ggaactgggc aaggtcaacg gctatatcga
      901 ggaaatgatg aacggccaga aggtcgtcaa ggtcttctgc catgaggaag ccgccatcaa
      961 ggagttcaac gagctcaacg atgagctgtt ccacagcgcc gacaaggcca acgcctactc
     1021 tctggtggca atgccggtca acggccagct gggcaatctg tcctacgtac tctgcgccat
     1081 catcggcggt gcgctggtca tcagcggctt tggcggcagc ctgtcgctgg gcggtctggc
     1141 cagcttcctg gtgctgaccc gccagttcaa ccagcccatc agccagatct ccatgcagct
     1201 gaactctgtt gtcatggcat tggccggcgg tgcgcgtatc tttgcgctgc tggatgaaaa
     1261 gccggaagtc aacgagggcg atatcaccct tgtccatgcg aagtacgccg ctgacgacac
     1321 cgtcaccgag accaacgagt ccaccggcat gtgggcgtgg aagcacatcg gagctgacgg
     1381 taagccccag tataccgagc tgaagggcga tatcgtcttt aaggatgtag acttcggcta
     1441 cgatgagaag aagatcgtcc tgcacgatat caacctgtac ggccgcccag gccagaagat
     1501 tgccttcgtc ggctcaaccg gtgcaggcaa gacgaccatc acgaacctca tcaaccgctt
     1561 ctacgatatc cagaagggcc agatcttgta cgacggccac gatatcaaat ccatcgagaa
     1621 ggacgcgctg cgttcctcgc tgggtatcgt cttgcaggac acccacttgt tcaccggcac
     1681 ggtcatggag aacatccgtt acggccgcct gaccgccacc gatgaggagt gcatggccgc
     1741 cgccaagctg gcgaacgccg ataccttcat caagcacctg cccgacggct acaacacgat
     1801 gctgaccggc gacggcacga acctgagcca gggtcagcgc caactgctgg ctatcgcccg
     1861 tgcagcggtc gcggatccgc cggtgctgat cctcgacgag gcaacttcct ccatcgacac
     1921 ccgcaccgaa aagctcgtgc aggacggcat ggacggcctg atgtatggcc gcacgacctt
     1981 cgtcattgcc caccgcctgt ccacggtgcg taactcggac tgcatcatgg tgttggagca
     2041 gggccgcatc atcgagcgcg gcacccacga cgagctgatt gcccagaagg gccgttacta
     2101 tcgcctgtac accggcaatt ttgccgagaa cagctaaacc gtcgaaaaag gtcgcaaaac
     2161 agcctgaaat caatcaaaac tgccgggata gcatccgctg tcccggcagt ttttgtgcaa
     2221 aatgccgcaa cagcgccaaa aagttgacga cgagggaaaa attggttata atagtatgcg
     2281 tagacacgca aaaagcgctg cctgaccacg cggccagagg gaaaacaggc cgccttacat
     2341 acactatttt taacaataca caggagggga ttccattatg gcacccaaga agaaaattgc
     2401 acagaccgtt attaccgaag gtaagttcta caccatcagt gccgctaacg gcaaagtcgt
     2461 cgaagtcgct gactataaca tcgacaacgg cgcgaagatc cagctgtggg acaacgccaa
     2521 cgccgagtgg cagcagtgga acttcgtcgc agcaggcgac ggagtttacc gcatccagaa
     2581 ccgtttcact gcaaagatgc ttgacctgga catgggcggc gtcagcgacg gcacccgcgt
     2641 ccaccagtgg gagggcgcac aggcttccag ccagctgtgg gtcgttgagc cgaccaacga
     2701 tggccgggtg aagatcaagt ccaacctggc cggcaagctg cttgacccgg gcatggctac
     2761 cgagaatggc accgtgctgc agatttgggc tgacgtcaac ggcgacaacc agttctggac
     2821 catcaacgaa gttacccgca agcccaagac cagtgtgaag gcttccgccg tcaaggccaa
     2881 ggctgccgcc gagaaggccg ccaccgaggc tgtcaaggct gccgagcctg ttgttgagaa
     2941 ggctgttaag gccgctaagc ccgccgtcga gaaggccgtc aaggccgccg agcctgtcgt
     3001 tgagaaggct gtcgagaccg caaagccggt cgtcgaggag actgtcaagg ccgcgaagcc
     3061 cgtggtcgag aaggctgtca aggctgccga gcctgttgtt gagaaggctg ttgaggccgc
     3121 aaagcccgtc gtcgaaaaga ccgttgaagc tgccaagacc gctgtcgcca agaccaacac
     3181 ccgcaagacc accaagggca agcgccgcaa gtaatttact ttgaaccttt ttcctgcaat
     3241 ttttgtgctg taacgagaaa accccgcgcc gtcaggatgc agttcctgac ggggcggggc
     3301 tattttttgt ttgtggctga aaagccttcc ccctgcgggg gaaggtggcc cgaagggccg
     3361 gatgaggggc gagtgtgcca gtattgccca cgaacgagct gcggcggaaa tgctgccctc
     3421 atcagtcacc tgcggtgaca gcttccccca cgggggaagc cttcagactt accccagcgc
     3481 cgcaatgctc tccttgatct tggcggcttt ctcctgggcg gcggccagct tggcgcgggt
     3541 ctcctcgacc agctttgcgg gggccttctc gacgaacttc gggttgttca gctggttggt
     3601 gaacatgccc agctccttct cggccttggt cagctccttg ttcaggcggg ccagctcctt
//
LOCUS       DBMDSQ010000207         3654 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_5826_length_3654_cov_1.777934, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000207 DBMDSQ010000000
VERSION     DBMDSQ010000207.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 3654)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3654)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3654
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5826_length_3654_cov_1.777934"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..422)
                     /locus_tag="ACZPAX_07635"
     CDS             complement(<1..422)
                     /locus_tag="ACZPAX_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HIR1113810.1"
                     /translation="MFWLYRYKGFSRTAARTVHTAVVLAGQQGCSQADTGHLLLALVQ
                     TARGTAADFLRRKRVTATALAGCSAARSLGSPRQLHSRDLAPELTKAMEFAVLGAHAA
                     SAAKAENEHLLCAMLEDSSCTASRWMASLGIELPQAAR"
     gene            575..2314
                     /locus_tag="ACZPAX_07640"
     CDS             575..2314
                     /locus_tag="ACZPAX_07640"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1113811.1"
                     /translation="MHKLMRYIKGYERQALLAPLFKMLEACFELFVPLVIASIIDTGI
                     KNQDAAFIWTRCGLLVLLAAIGLASSLTAQWFSATAALGFGTALRKDLFRHIDTLSYS
                     ELDGIGTPTLVTRMTSDINQVQNGVNLTLRLLLRSPFIVIGALIMAFSISPKLTLLFI
                     GVTIMVSLIIWAIMRVTVPIYHEAQNGLDRVTLLTRENYVGARVVRAFARQADELAAF
                     VETNDHLKKLQFAAGRISALMNPLTYLVVNLAVVALLLRGGVEVDAGALTQGEVIALI
                     NYMSQILLSLLRLADLVVSVTRALASGMRVNEILNTHTTMEDPGTATLDIANAADAAK
                     FDHVNFTYKGAGAPSLTDISFTAKVGETIGVIGGTGSGKSTLIDLVCRFYDADNGGVT
                     LFDHDVKQYSFAQLRGMVGIVPQQAVLFTGTIRDNMQWAAPGATDEEIWQALEIAQAA
                     EFVRGKPGMLDAPVETAGRNFSGGQRQRLTIARALVPKPKILILDDSASALDFATDAA
                     LRKAIKEETSGMTVFIVSQRAASVQRADHILVLDDGKLVGDAPHAELLQSCEVYKEIC
                     LSQLSKEEVAKTL"
     gene            2311..>3654
                     /locus_tag="ACZPAX_07645"
     CDS             2311..>3654
                     /locus_tag="ACZPAX_07645"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1113812.1"
                     /translation="MSKQKPKLNQNTIHRVLKLIRPYTGMVILTLTFALITVVTTLLA
                     PVLSGKAVDMILGPGEVDFVGLGKIALVMAGTIACTALSQWLMNVVNNRITFQVVRDI
                     RVKAFEQLEILPLKYMDAHRPGDAISRITTDVEQFSDGLLMGFTQLFTGVLTIFGTLG
                     VMIFIDWRIALVVVALTPLSIFVARFIATHTYSMFKVQSETRAEMTSLVEELVGNEHM
                     VRAFGYESRAEERFDKINLDLQKCGVKATFFSSTTNPCTRFVNALVYAAVGVLGAFVA
                     IARGITVGQLSVFLNYANQYTKPFNDISDVMTELQNALACAQRVFDLIDETPITPDSP
                     DAVALPHGAGSVEFDHVKFRYVDDVPLIEDMNLKVAPGQRIALVGPTGCGKTTLVNLL
                     MRFYEINGGTLRVDGHPIDTVTRDSLRGNLGMVLQETWLKAGTIAENIAYGKPDAM"
BASE COUNT          730 a         1075 c         1084 g          765 t
ORIGIN      
        1 tcccgcgcgg cctggggcag ctcgatccct aaggacgcca tccaccggct ggccgtgcag
       61 gacgaatcct cgagcatcgc gcagagcagg tgctcattct cggccttagc ggcgctggcg
      121 gcgtgggccc ccaacacggc aaattccatg gccttggtca actcgggggc caggtcacgg
      181 gaatgcagct gccgggggct gcccaggctt cgggcggcgc tgcacccggc cagcgccgtg
      241 gctgtcacac gcttgcggcg cagaaaatcc gccgccgtgc ctcgagcagt ttgcaccaac
      301 gccagcagca ggtggccggt gtcggcctgg gaacacccct gctgcccggc cagcactacc
      361 gccgtatgca cggtgcgtgc cgccgtgcgg gaaaaacctt tatatcggta aagccagaac
      421 atctgcatat cctccctcaa caatgtgtcc attgaaagta tagacgccgg gcaaataaat
      481 agtcatcgaa atatgtagct tcatgcaatg acaagcggga aaatatgtga tataataaac
      541 cgatattgtt aatttttggt ttgaggtggt ttcaatgcac aaattgatgc gctacatcaa
      601 gggctacgag aggcaagccc tgttagcgcc gctgttcaag atgctggaag cctgctttga
      661 gttgttcgtg ccgctggtca ttgccagcat tattgacaca ggcatcaaaa atcaggacgc
      721 agctttcatc tggacgcgct gcggtctgct ggttttgctg gcggccatcg gccttgccag
      781 cagtttgacg gcccagtggt tctccgctac ggcggcgcta ggctttggta cggcgctgcg
      841 caaggacttg ttccgccata tcgacacact gagttacagc gaactggatg gcatcggcac
      901 cccgacactg gtcacccgta tgaccagcga catcaatcag gtgcagaacg gcgtcaacct
      961 gacgctgcgc ctgctgctgc gttcgccgtt catcgttatc ggtgcgctga ttatggcgtt
     1021 ctccatcagc ccgaagctga cgctactgtt tatcggtgtg acgattatgg tgtcgctcat
     1081 catatgggcc atcatgcgcg tgacggtgcc tatctaccac gaggcacaga atggtctgga
     1141 tcgcgtgacg ctgctgaccc gcgaaaacta cgtaggtgcc cgcgtcgtgc gtgcctttgc
     1201 ccgccaggcg gacgagctgg ccgcctttgt ggagacgaac gaccacctga aaaaattgca
     1261 gtttgcagcc gggcgtatct ccgcattgat gaacccgctg acctatctcg tcgtcaacct
     1321 ggctgtggtg gccctgctgc tgcgcggcgg tgtcgaggtc gatgccggtg cgctgaccca
     1381 gggcgaggtt attgccctta tcaactatat gagccaaatc ttgttgagtc tgctgcgcct
     1441 ggcggacctg gtcgtctcgg tcacgcgtgc cctggccagc ggtatgcgcg tcaatgaaat
     1501 tttaaacacc cataccacga tggaggaccc tggcacggcg acgctggaca ttgcgaatgc
     1561 ggccgacgcg gcgaagttcg accatgtcaa cttcacctac aagggggcag gcgcacccag
     1621 cctgaccgat atctcgttta cggccaaagt cggtgagacc atcggtgtaa tcggcggcac
     1681 cggcagcggc aaatcgacct tgattgatct tgtctgccgt ttctatgatg ccgataacgg
     1741 cggcgtgacg ctgtttgacc acgatgtaaa gcagtacagc tttgcacagc tgcgcggcat
     1801 ggtgggcatc gtgccccagc aggccgtgct gttcaccgga accatccgcg acaatatgca
     1861 gtgggccgcc cccggtgcga ccgacgagga gatttggcag gcgctggaga ttgcccaggc
     1921 ggcggagttc gtgcgcggca aacccggcat gctggacgct ccggtcgaga ctgcaggccg
     1981 caacttctca ggcgggcagc gccagcgcct gacgattgcc cgcgcactgg tgccaaagcc
     2041 gaagattctg attctcgatg acagtgcctc agcactggat tttgctactg acgcggcgct
     2101 gcgcaaggct attaaagagg aaacgagcgg tatgactgtg ttcatcgtct cccagcgtgc
     2161 ggccagcgtg cagcgtgccg accatatcct ggtgttggac gacggcaagc ttgtgggcga
     2221 tgctccccac gccgagctgc tgcaaagctg cgaagtttat aaggaaatct gcctgagtca
     2281 gctgagcaag gaggaggtgg ccaaaacgct atgagcaagc aaaaaccaaa actgaaccag
     2341 aacaccatcc atcgcgtttt gaagctgatc cgcccctaca ccggcatggt catcctgacg
     2401 ctgacatttg cgttgatcac agtggtcact accctgctgg ccccggtgct gtccggcaag
     2461 gccgtggata tgattctcgg ccccggtgag gtcgacttcg tgggccttgg caaaatcgca
     2521 ctggtcatgg cgggcacaat cgcctgcacg gcgctgtccc aatggctgat gaatgttgtg
     2581 aacaaccgca tcaccttcca ggtcgtgcgg gacatccgcg tcaaggcgtt tgagcagctg
     2641 gaaattttac cgctgaaata tatggacgcc caccgccccg gcgacgctat cagccgcatc
     2701 actaccgatg tagagcagtt cagcgacggt ttgttgatgg gttttaccca gctgtttacc
     2761 ggcgtgctga cgatttttgg cacgctgggc gtgatgattt tcattgactg gcgcattgcg
     2821 ctggtggtcg tggcgctgac gccactgtcc atctttgtgg cgcggttcat tgccacccat
     2881 acctattcta tgttcaaggt gcagagcgag acccgcgccg agatgaccag cctggtggaa
     2941 gagctggttg gcaatgagca tatggttcgt gccttcggct acgaaagccg cgccgaggaa
     3001 cgctttgaca agatcaacct cgatttgcag aagtgcggcg tcaaggcgac gttcttctcc
     3061 tccaccacga acccctgcac ccgatttgtc aatgcgctgg tctacgccgc cgtcggcgtg
     3121 ctgggcgcat tcgtcgccat tgcgcgcggc atcacggtcg gtcagctgtc ggtgtttttg
     3181 aactacgcga accagtacac gaagcccttc aacgatatct ccgacgttat gaccgaattg
     3241 cagaacgccc ttgcctgcgc acagcgcgtg tttgacctca tcgatgagac acccatcacg
     3301 ccagattccc ctgacgcggt ggcactgccc cacggtgcgg gcagcgtcga gttcgaccac
     3361 gtcaagttcc gctacgtgga cgatgttccc ctgattgagg acatgaattt gaaggtcgca
     3421 cccggccagc gcatcgccct tgtcggcccc accggctgcg gcaagacgac cctggtcaac
     3481 ctgctgatgc ggttctatga gatcaacggc ggcacgttgc gggtcgatgg acaccccatt
     3541 gataccgtga cccgggacag cctgcgcggc aacctgggta tggtcttgca ggaaacgtgg
     3601 ctgaaagccg gtacgattgc cgagaacatt gcctacggca agccggacgc aatg
//
LOCUS       DBMDSQ010000208         3639 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_5852_length_3639_cov_1.800735, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000208 DBMDSQ010000000
VERSION     DBMDSQ010000208.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 3639)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3639)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3639
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5852_length_3639_cov_1.800735"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..946)
                     /locus_tag="ACZPAX_07650"
     CDS             complement(<1..946)
                     /locus_tag="ACZPAX_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HIR1113813.1"
                     /translation="MTPLQKNILNAVKRRPMTLRELQNNLQVDNSRLRTSVSAMVATG
                     ELSMRNGTYAPGFATYVNANAPEAIPCTLVKLAARFGFASRDDGEGDIFIPGRALHGA
                     MPQDKINVKLFDHPRVEGSSEGEVVEVTVPNNRFAGTVCLSEDGRLAVEPDGCRDVKF
                     LLAKQGSEGVHLGDKVGFLITHRGNRHSDHRAAVVEKFGSSDYASECAKAILYGRNVR
                     LEFPPEVLEEAHAYDDATIDPTEAAKRLDLRGIPIFTIDSAETKDIDDAISLQKLDDG
                     YELGVHIADVSHYVRPGSALDEEAFERATSIYYADKVIP"
     gene            complement(1103..1351)
                     /gene="secG"
                     /locus_tag="ACZPAX_07655"
     CDS             complement(1103..1351)
                     /gene="secG"
                     /locus_tag="ACZPAX_07655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HIR1113814.1"
                     /translation="MNWYEIALGVVLVVVSVLIIVFTLAQEQKGQGLSGAIMGESSAT
                     MAAGRERGVDAKLAKLTKVCGIVFFVVTLVVCVLSARL"
     gene            complement(1472..2446)
                     /gene="bsh"
                     /locus_tag="ACZPAX_07660"
     CDS             complement(1472..2446)
                     /gene="bsh"
                     /locus_tag="ACZPAX_07660"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008399830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HIR1113815.1"
                     /translation="MCTAATYKTEDFYFGRNLDYEFSYGDEVTVTPRAYPFALRCMGN
                     FRTKYAMIGMAYVAGEYPLYYDAVNEKGLGIAGLNFVGNAVYHPAAEGKNNVTQFELI
                     PWLLGSCATLAEARTLLEKTNIVNIPFSDQLPLAQLHWLIADKTGSIVVESTADGLHI
                     YDDPVGVLTNNPPFPQQLFALNNYRALSPRTPASTFAEGLDLTAYSRGLGGLGLPGDL
                     SSQSRFVRAAFVRMNAKSGSGEAESVNQFFHILHSVEQQRGCCELDGGKYEITLYTSC
                     CNADKGIYYYTTYGNHQITAVDMHRENLDGTKLTRYPLVQGEQIALQN"
     gene            complement(2684..2800)
                     /locus_tag="ACZPAX_07665"
     CDS             complement(2684..2800)
                     /locus_tag="ACZPAX_07665"
                     /inference="COORDINATES: protein motif:HMM:NF012948.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HIR1113816.1"
                     /translation="MPTIPQTGDNSSVTLLFALLLLSGGAYLGMAASKKAKQ"
     gene            complement(2973..>3639)
                     /locus_tag="ACZPAX_07670"
     CDS             complement(2973..>3639)
                     /locus_tag="ACZPAX_07670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YveK family protein"
                     /protein_id="HIR1113817.1"
                     /translation="ISAILKWLTIVTVLLFFVLGGVVGFVKAKVFKEEKYTSTALLFV
                     DLNRDAIDTSNDMNESSKISMLANSFSQIVSSDSVIAPIAKQYALDSEDIDVENEDAV
                     NTKVDNLRENITVTVPSDTQTINVSVTDDNAERCQSICETIVDTGIKAMDSATNYATI
                     SIVSPASEGKKEKSEAAGAAASALTMAAIFLVVAILIVAVRELGIAYKAHEAAKQEEE
                     KKN"
BASE COUNT          740 a         1044 c         1056 g          799 t
ORIGIN      
        1 tcgggatgac cttgtcggcg tagtaaatgc tcgtcgcgcg ctcaaaggct tcctcatcca
       61 gggcggaacc ggggcgcaca taatggctga cgtcggcaat gtggacgccc agctcgtagc
      121 cgtcatccag cttttgcagg ctgatggcat cgtcgatgtc cttcgtctca gcggagtcga
      181 tggtgaagat ggggatgccg cgcaggtcca gccgcttcgc agcctcggtc gggtcaatcg
      241 tggcgtcgtc ataggcgtgg gcctcttcca gcacctcggg cgggaactcg agccggacgt
      301 tgcggccgta cagaatggct ttggcgcact cggaggcgta gtcggaggag ccgaactttt
      361 cgacgacggc ggcgcggtgg tcgctgtggc ggttgccgcg gtgggtaatg aggaaaccga
      421 ccttgtcgcc caggtggacg ccctcgctcc cctgtttcgc cagcaggaac ttcacatcgc
      481 ggcagccgtc cggctcaacg gccaggcggc catcctcaga caggcagacc gtaccggcaa
      541 agcggttgtt gggcacggtg acttcgacga cctcgccctc agaggaaccc tccacgcggg
      601 gatggtcgaa cagcttgacg ttgatcttgt cctggggcat cgcgccatgc agcgcgcggc
      661 ccgggatgaa aatatcgccc tcgccgtcgt cgcggctggc aaagccgaag cgggccgcca
      721 gcttgaccag cgtgcagggg atggcctcgg gggcattggc attcacatag gtggcaaaac
      781 ccggggcata ggtgccgttg cgcatcgaca gctcgccggt ggcgaccatg gccgacacgc
      841 tggtgcgcag gcgggaatta tccacctgca aattgttctg cagctcgcgc agcgtcatgg
      901 ggcggcgctt gacggcgttt aaaatgtttt tctgtaacgg tgtcatgtgt actcctttaa
      961 tttacgtcgc accgtcgtcc ggcggtgccc taccagtttg acttctttat atactttcac
     1021 aaaaaacggc ccgccggggc gctgcctgca aaggcctaaa gcgcttggcg gacccttttg
     1081 ttttgtgctt accctgccaa aatcacaggc gtgcgctgag gacgcagacg accagcgtga
     1141 cgacaaagaa aacaatgccg cagaccttgg tcagcttggc cagcttggcg tccacgccgc
     1201 gctcacggcc agcagccatc gtggcggagc tttcgcccat gatcgcgcca gacagaccct
     1261 ggcccttctg ctcctgagcg agggtaaaga cgatgatcaa aacggatacg acgacgagga
     1321 cgacgccgag agcgatctca taccagttca ttgattagca ctccttctat ttctatccac
     1381 gccgccatga cgtgaaaagt ctcatgcacc gtacagtata gcatagattc ctgtacgatg
     1441 caagcgcttt tttcacgttt tgtgcgtaaa atcagttttg cagcgcaatt tgctcgccct
     1501 gcaccagcgg gtagcgggtc agcttggtgc cgtccaggtt ttcccggtgc atatccacag
     1561 cggtgatctg gtggttgccg taggtcgtgt agtaataaat gcctttgtcg gcgttgcagc
     1621 aactggtgta gagcgtgatc tcatacttgc cgccgtccag ctcacagcag ccgcgctgct
     1681 gttcgaccga gtgcaggatg tggaagaact ggttgacact ctcggcctcg ccgctgccgg
     1741 atttggcgtt catacgcaca aaggccgcac ggacaaagcg ggactggctg gacaggtcgc
     1801 ccggcaggcc cagcccgccc aggccgcggc tgtacgcggt caggtccagc ccctcggcaa
     1861 aggtactggc gggtgtgcgg ggcgacaggg cacggtagtt gttcagcgcg aaaagctgct
     1921 gcgggaacgg cggattgttt gtcagcacgc ccaccgggtc atcataaatg tgcaggccgt
     1981 cggcggtgga ttccaccacg atgctgccgg tcttgtcggc aatcagccaa tgcagctggg
     2041 ccaggggcag ctggtcactg aatggaatat tgacgatgtt tgtcttttcc agcagcgtgc
     2101 gggcctcggc cagggtggcg cagctgccca gcagccaggg gatcaattca aactgtgtga
     2161 cgttgttttt accctcagcc gccggatggt agaccgcgtt gcccacaaag ttcagccccg
     2221 caatgccgag gcccttctca ttgacagcgt cgtaatacag cgggtactcc cccgccacgt
     2281 aggccatgcc gatcatggcg tatttcgtgc ggaaattccc catgcagcgc agggcaaacg
     2341 gataggcgcg cggcgtgacc gtgacctcgt cgccgtagga aaattcatag tccagattgc
     2401 ggccaaagta gaaatcttcg gttttatacg ttgcagcggt acacatggca agctcccctt
     2461 tttataaaaa atgtagtatg tttcttatat tgagatgtag gggcgaacat tgttcgcccg
     2521 cagcacacga cggcagccgc aaagtcccgc ggtcgcacgc tgtgcgcccc tacacatttg
     2581 gcgagtcaat gcaactagtg taccacgccg gcaaggaata atccaccgag aaattgtaaa
     2641 caaatggcgg caggggtgtt tcctgccgcc atttggctgt atattactgt tttgcttttt
     2701 tggatgccgc catacccagg tatgcgccgc cgctcaacag gagcagcgcg aacagcagcg
     2761 tgacactgct gttatccccc gtctggggaa tcgtgggcac tgccttgccg tcagaaactt
     2821 ttcctcattt ccgcagtatt ttaagcactt ttaactgtgc gcccctatcc aaaatttgtc
     2881 aagaaacgca cagaaattaa acatccgcgc aaaaatcgca caaaaaagcg caccggctta
     2941 ccgccgatgc gcttgcagac tatgctataa aatcagtttt tcttttcttc ttcctgcttg
     3001 gcggcctcgt gtgccttgta ggcaatgccc agttcacgca cggcgacaat cagaatcgca
     3061 acgaccagga agatggcggc catcgtgagg gcggaagctg ccgcacctgc ggcctcgctc
     3121 ttctcctttt tgccctcgct agccggggaa acgatgctga tggtggcgta gttggtagcg
     3181 ctgtccatcg ccttgatgcc ggtatccacg atggtctcgc agatgctctg gcagcgctct
     3241 gcattgtcat cggtcaccga aacattgatg gtctgcgtgt cgctgggaac cgtcacggta
     3301 atattctcgc gcagattgtc caccttggta ttgacggcgt cctcgttctc cacatcgata
     3361 tcctcgctgt ccagcgcgta ctgcttggcg atgggcgcaa tgacggagtc actggaaacg
     3421 atctgcgaga aggagtttgc cagcatactg atcttggagg actcgttcat atcgttactg
     3481 gtgtcgatcg catcacggtt caggtcaacg aacagcaacg ccgtggaggt atatttctct
     3541 tccttaaata cctttgcctt gacaaagccg accacaccgc cgagcacaaa gaacaacaat
     3601 acggtcacaa ttgttagcca ctttaaaatt gcggatatc
//
LOCUS       DBMDSQ010000209         3590 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_5938_length_3590_cov_1.943537, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000209 DBMDSQ010000000
VERSION     DBMDSQ010000209.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 3590)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3590)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3590
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5938_length_3590_cov_1.943537"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..562
                     /locus_tag="ACZPAX_07675"
     CDS             <1..562
                     /locus_tag="ACZPAX_07675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113818.1"
                     /translation="EVELVRKDLIAMAAQAEARRDYLPSVIGDVDVFKRNLLASMPKP
                     KFADYMADSLVWLCSFALASAATYTVMGRPWDCYYDAVWLVLCIAIMMPSAWLLQRIV
                     PAEWAASTEKGVRYGAITAAMMLLWFVEFWFVDKWITPLLPAVGLPNLAAVILFGAAL
                     ATLLIWRNHRRNELAEKRPWRDVMEK"
     gene            complement(750..1820)
                     /gene="leuB"
                     /locus_tag="ACZPAX_07680"
     CDS             complement(750..1820)
                     /gene="leuB"
                     /locus_tag="ACZPAX_07680"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015565545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HIR1113819.1"
                     /translation="MQKNITLIYGDCSSPEIVTQAVRVLDKIAEKHGHTFTYTRAYMG
                     GEAIDKFGDPLPAAELEKCKNADSVLLGAIGGPKWEGLPGDQRPEKGLLRLRSGMGLY
                     ANNRPAKIWPQLADASPLKKSIVDKGIDFIVVRELIGGVYFGEHKTIEENGEKVAIDV
                     MPYAEHEIERIGRIGFETAMKRRKKLTCVDKANVLDTSRLWRAVMHRLQVEYPEVEYS
                     EMFVDNCAMQICKNPAQFDVIVTENMFGDILSDEASEVTGSIGMVPSSSLGQGTCGLY
                     EPIHGSAPDIAGQDVVNPIACILSAAMMLRYSFDMPAEADEIEAAVNAVLDEGYRTAD
                     MMAPGCTRVGCTEMGNKILEHI"
     gene            complement(1940..2422)
                     /gene="leuD"
                     /locus_tag="ACZPAX_07685"
     CDS             complement(1940..2422)
                     /gene="leuD"
                     /locus_tag="ACZPAX_07685"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013484849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HIR1113820.1"
                     /translation="MNARGPVFKYGDNIDTDVIIPARYLNTQSAAELASHCMEDIDKT
                     FVTRVKAGDIMVGGENFGCGSSREHAPVAIKASGISCVIAKSFARIFYRNAINIGLPI
                     LECPAASEAINEGDTVSINFDTGVITDETTGQTFQAAPFPPFIQNIIAKGGLMNSLKD
                     "
     gene            complement(2426..>3590)
                     /gene="leuC"
                     /locus_tag="ACZPAX_07690"
     CDS             complement(2426..>3590)
                     /gene="leuC"
                     /locus_tag="ACZPAX_07690"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005942255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HIR1113821.1"
                     /translation="LGNDITAPVAINEFEKAGFDSVFDNTRVNIVLDHFVPNKDIKSA
                     AQSKQCREFADKYDILNFFDVGRMGVEHALLPEQGIVTAGDCIIGADSHTCTYGAVGA
                     FSTGVGSTDMAAGMAKGMAWFKVPAAIKVELKGQLHKPVTGKDVILHLIGQIGVSGAL
                     YKSLEFVGEGVKTLTMEDRLCICNMAIEAGAKNGIFPVDEVTEAYLKGRSQRPWTKYE
                     ADPDAEYERTIVIDLDALEPTVSYPHLPENTHLAKEGKDIKIDQIVIGSCTNGRIEDM
                     EAAYEILKGKHIAKGVRGIIIPATMAVYKECILRGYTEAFIDAGCIVSTPTCGPCLGG
                     YMGILAAGERCVSTTNRNFVGRMGHVESEVYLASPATAAASALTGYITDPREV"
BASE COUNT          637 a         1047 c         1129 g          777 t
ORIGIN      
        1 tgaggtcgaa ctggtgcgca aagatctgat tgccatggcg gcacaggccg aggcccggcg
       61 cgactatctg ccgtcggtca tcggggacgt agatgttttt aaacggaatc tgctggcaag
      121 catgcccaag ccgaagtttg cggattacat ggcagacagc cttgtgtggc tgtgctcgtt
      181 tgcgttggcc tccgccgcga cctatacggt gatgggccgc ccgtgggact gctattatga
      241 cgcggtctgg cttgtgctct gcattgccat tatgatgccc agtgcgtggc tgctgcagcg
      301 cattgtcccc gccgagtggg cggcctctac cgaaaaaggt gtgcggtacg gcgctattac
      361 agccgctatg atgcttttgt ggttcgtgga gttttggttc gtcgacaaat ggatcacgcc
      421 gctgctgccc gcggtgggcc tgcccaacct tgcggcagtc atcctgttcg gcgcggcact
      481 ggcaacgctg cttatctggc gcaaccaccg ccgcaacgag cttgccgaaa aacggccctg
      541 gcgggatgtg atggagaaat aaaagtgtcc cccgatggtg cggaaccatc gggggacgct
      601 ttgccttccc ctgcggggga aggtggccga acgcagtgag gtcggatgag gggcgagctt
      661 tccgcaattg cccataaacg ggttgctgcg ggaaggtcgc ccctcatcag tcaccttcgg
      721 tgacagcttc ccccgcgggg ggaagccttt taaatatgct ccaaaatctt gttgcccatc
      781 tcggtgcagc ctaccctggt gcagccggga gccatcatgt cggcggtgcg gtagccttcg
      841 tcgagaacag cgttgacagc cgcctcgatc tcgtcggcct cggcgggcat atcgaagctg
      901 taccgcagca tcatggccgc ggagaggatg caggcaatcg ggttgacgac gtcctgcccg
      961 gcaatatcgg gcgcagagcc gtggatcggc tcatacaggc cgcaggtccc ctgccccagt
     1021 gaggaggacg gcaccatgcc gatggaaccg gtcacttcgc tggcttcgtc agagaggata
     1081 tcgccgaaca tattttcggt gacgatgacg tcaaactggg cgggattctt gcagatctgc
     1141 atagcgcagt tgtccacaaa catttcactg tactcgacct cggggtactc gacctgcaga
     1201 cgatgcatga cggcgcgcca caggcggctg gtgtccagca cgttcgcctt gtccacgcag
     1261 gtcagctttt tgcggcgctt catggccgtc tcaaagccga tgcggccgat gcgctcaatt
     1321 tcatgttcgg cgtagggcat cacgtcgatg gcgaccttct cgccgttttc ctcaatcgtc
     1381 ttatgctcgc caaagtagac gccgccaatc agctcacgca cgacgataaa gtcaatgccc
     1441 ttgtccacaa tggacttttt cagcggggag gcatccgcca actggggcca gatcttggcc
     1501 gggcggttgt tggcatacaa tcccatgccg gaacgcaggc gcagcaggcc cttctcaggg
     1561 cgctggtcgc cgggcaggcc ctcccacttg gggccgccga tggcccccag caggacgctg
     1621 tcggcgttct tgcacttttc cagttccgca gcgggcagcg ggtcgccgaa tttatcaatg
     1681 gcctcgccgc ccatgtaggc gcgggtatac gtaaacgtat ggccgtgctt ttctgcaatt
     1741 ttatccagca cgcgcaccgc ctgggtcacg atctccgggc tggaacagtc gccgtagatc
     1801 agagtgatgt ttttctgcat agttctgttc tccctctcgt tgctattgta ggggcgggac
     1861 atgtcccgcc ctcagccttt ccatcacagc acttttgccg taaagccgcg ggccgggcat
     1921 gcccggcccc tacagggcat cagtccttca agctgttcat caggccgccc ttggcgataa
     1981 tgttctggat gaacggcggg aagggcgcgg cctggaaggt ctggcccgtg gtttcgtcgg
     2041 taatgacgcc ggtgtcaaag ttgatgctga ctgtatcgcc ctcgttgatt gcttcgctgg
     2101 cggcggggca ttcgagaatc ggtaaaccga tgttgatggc gttgcggtag aagatgcggg
     2161 caaagctctt ggcgataacg cagctgatac cggacgcctt gatggcaacg ggggcatgct
     2221 cgcgggagga gccgcagcca aagttctcac cgcccaccat gatgtccccc gccttgacac
     2281 gggtaacaaa cgttttatcg atatcttcca tacagtggct tgccagctcg gcggcactct
     2341 gggtattcag gtagcgggcc gggatgatca cgtcggtgtc aatgttgtcg ccgtatttaa
     2401 aaacaggacc tcttgcgttc atagctcaaa cctccctcgg atcagtgatg tagccggtca
     2461 gggcgctggc cgccgccgtg gcagggctgg cgaggtagac ctcggactcc acgtggccca
     2521 tgcggccgac gaagttgcgg ttggtggtgg agacacagcg ctcgcccgcc gccaggatac
     2581 ccatgtagcc gcccaggcag gggccgcagg tgggcgtgga gacgatgcag ccagcgtcga
     2641 tgaaagcctc ggtgtagccg cgcagaatgc actccttgta gacggccatc gtggcgggga
     2701 tgatgatgcc gcgcacgccc ttagcgatgt gcttgccctt caggatctcg taggcggcct
     2761 ccatgtcctc aatgcgtccg ttggtgcaag agccaatcac gatttggtcg atcttgatgt
     2821 ccttgccctc tttggcaaga tgcgtgttct cgggcagatg cgggtagctg accgtcggct
     2881 cgagcgcgtc gaggtcaatc acgatggtgc gctcatactc ggcgtcgggg tcggcctcgt
     2941 acttggtcca cgggcgctgg ctgcggcctt tgaggtaggc ttcggtcact tcgtccacgg
     3001 ggaagatgcc gtttttggca ccggcctcga tggccatgtt gcagatgcag aggcgatcct
     3061 ccatcgtcag ggtcttgacg ccctcgccga caaattccag actcttgtac agtgcgcccg
     3121 agacgccgat ttgaccaatc aggtgcagaa tgacatcctt gccggtgacg ggcttgtgca
     3181 gctgaccctt cagctcgacc ttgatggcgg cggggacctt gaaccaggcc atgcctttcg
     3241 ccatgccggc ggccatatcg gtggagccga cgccggtgga gaacgcgccc accgcgccgt
     3301 aggtacaggt gtggctgtcg gcgccgatga tgcagtcacc ggcggtcacg atgccctgtt
     3361 cgggcagcag tgcgtgctca acgcccatgc ggcccacgtc gaaaaaattg aggatgtcgt
     3421 acttgtcggc gaactcgcgg cactgcttgg actgggccgc gctcttgatg tccttgttgg
     3481 ggacaaagtg atccagcaca atgttgacgc gggtgttatc gaacaccgag tcgaaccctg
     3541 ctttttcaaa ttcgttgatg gccaccgggg cggtgatatc attgccgagc
//
LOCUS       DBMDSQ010000210         3559 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_6010_length_3559_cov_1.719780, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000210 DBMDSQ010000000
VERSION     DBMDSQ010000210.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 3559)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3559)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3559
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6010_length_3559_cov_1.719780"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     regulatory      219..314
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF02680"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1-III riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="pre-queuosine1"
                     /db_xref="RFAM:RF02680"
     gene            321..845
                     /locus_tag="ACZPAX_07695"
     CDS             321..845
                     /locus_tag="ACZPAX_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005924938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HIR1113822.1"
                     /translation="MNQKKLSVHSLVRCAVIAAVYVVVCLVLAPFSYGAIQVRVAEAL
                     CLLPVFGAEYIVGVTLGCFLANLLGSTVIDVVFGTLATLLACLLTYKLRDIRVKGLAI
                     PASLPPVVFNMIIVGAFEITFFFSDGAPTAALAAFNAVTVGIGELISCTILGVALVKL
                     IESNAALHKFFTEE"
     gene            859..1506
                     /locus_tag="ACZPAX_07700"
     CDS             859..1506
                     /locus_tag="ACZPAX_07700"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007045869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR1113823.1"
                     /translation="MRPELILWDWNGTLLDDVELCENALNRLLQRYGYPQRYDHEQYR
                     AIFGFPVEDYYVRAGFDFTRHSFAELAESYMEDYIPASAACPLMAGAVDALEAFKNAG
                     LRQVILSASPVTTLTRQVEERGATPYFDRLLGLGDIYAKSKVEVGLAYLQEAGFDPAK
                     AVMIGDSVHDFEVAQALGVGCVLQSSGHQPPETLRRTGAPVVAGLREAAELILKK"
     gene            1589..2494
                     /locus_tag="ACZPAX_07705"
     CDS             1589..2494
                     /locus_tag="ACZPAX_07705"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR1113824.1"
                     /translation="MSDRVKGACLTMGGAACWGVSGCMGQYLFTREGMDSTWLVPIRL
                     FLAGLILCAFFFVKNRKMLFDPWNFKKNPRNVVDLLIYGLAGVSCCQFTYFLTIQLSS
                     AGMGTILQDLSPVIILLVVCIQQRRGPRVFEIFSILLALVGVFLITTHGSLTDLAISP
                     AALASGVVSACCVAIYTMWPKKLQEQYPTPMLQGWAFLMGGTMFALIFRPWTINYVPT
                     PMGVFGIFTVVVLGNVLAFSLYMSGVPLIGPQKSSLYSFAEPVTAAIISTLVLGSPFT
                     LWDALGFGCIFLMLVLLSLPAKTKA"
     gene            2757..>3559
                     /locus_tag="ACZPAX_07710"
     CDS             2757..>3559
                     /locus_tag="ACZPAX_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005925040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIR1113825.1"
                     /translation="MSFFEKLFGSFSDKELKRINPIKDKVLALEPEMAKLTDEELQAK
                     TTEFKERLAKGETLDDILPEAFAVCREADWRVLGMKPYPVQIIGGIVLHRACIAEMQT
                     GEGKTLVATMPTYLNALTGEGVHVVTVNDYLARRDSEWMGKVYRFLGLTVGLVVHGVE
                     STDRKKAYEADVTYGTNNEFGFDYLRDNMVVYKANMVQRGHAFAIVDEVDSILIDEAR
                     TPLIISGKGEDSSVMYKRADDFAKTLKKSVIVELDDKVEAEEQVDGDYVV"
BASE COUNT          696 a          996 c         1069 g          798 t
ORIGIN      
        1 cacaccgtag cgggcggcga cgtccgtgca gattatacca taccaagagc tattagggaa
       61 ctatgacatt agagcctgca acaaactatt taatcgaatt ataccatacc aagagctatt
      121 agtaaatcat cccccccaat aaaatcccgc taaccccaaa atttcttccc cgcaggcctt
      181 gaaacggtct gcggggtttg ctataattta aatttgttga gcaacttagg attttaggct
      241 ccaccgcggc ttgctgcggt gcaaaccccg caagcctggc ggtcttgcgc gcggtcaaaa
      301 tctatccgcc ggagtattgt atgaatcaga aaaaactgtc cgttcacagc cttgtgcgct
      361 gtgctgtcat tgccgcagtg tatgtggtcg tctgcctggt gctggccccc ttcagctatg
      421 gggctatcca ggtgcgcgtg gccgaggccc tgtgcctgct gccggttttc ggcgcggagt
      481 acatcgtcgg cgttacgctg ggctgctttt tggccaacct gctgggctcc accgtcattg
      541 acgtggtgtt cggcacgctg gctaccctgc tggcctgcct gctgacttac aagctgcgtg
      601 atatccgcgt aaaagggctg gcaattccgg catccctgcc gcctgttgtt tttaacatga
      661 tcattgtggg cgcgttcgag atcaccttct tcttctcgga cggcgcaccc acggcggccc
      721 tggcggcgtt caacgccgtt acggtgggca tcggagaact gatcagctgc acgattttgg
      781 gcgtggccct cgtcaagctg attgagagca acgcggccct gcacaagttc tttacagaag
      841 aataacgagg taacttctat gcgcccggaa ctgattttgt gggattggaa cggtaccctg
      901 ctggatgatg tcgaactctg cgaaaacgcg ctcaaccgtc ttttgcagcg ctacggctac
      961 ccccagcggt acgaccacga gcagtaccgg gcgatttttg gcttcccggt cgaggactac
     1021 tacgtccgcg ccgggttcga cttcacccgc cacagctttg ccgagctggc ggaaagctac
     1081 atggaggatt acatcccggc cagcgccgcc tgtccgctga tggcaggggc cgttgacgcg
     1141 ctggaagcgt tcaaaaatgc cgggctgcgg caggtgattc tgtcggcaag cccggtcaca
     1201 acgctgaccc gccaggtgga agaacgcggt gcgacgccgt attttgaccg cctgctggga
     1261 ctgggcgata tctatgccaa aagtaaggtg gaggtcgggc tggcctattt acaggaggca
     1321 ggtttcgacc cggcgaaggc cgtgatgata ggtgattccg tccatgattt tgaggtggcc
     1381 caggcgctgg gtgtgggctg tgtgctgcaa tcgagcggcc accagccgcc cgagacactg
     1441 cgccggaccg gtgcaccggt ggtggcaggg ctgcgggaag cggcggagtt gattttgaaa
     1501 aagtgagccg tgcggcgcgg ggcgtcaagg gcgccgcccc ctacaaccgt agagaggcat
     1561 tggaaatgat aggcagagag gtacaaaaat gtctgaccgt gtaaaaggcg cctgcttgac
     1621 gatgggcggc gcggcgtgct ggggtgtttc gggctgcatg gggcagtacc tgttcacccg
     1681 ggaagggatg gattccacct ggctggtgcc catccggctg tttctggcgg ggctgatttt
     1741 atgtgcgttc ttttttgtca aaaaccgcaa aatgctgttt gacccatgga atttcaagaa
     1801 aaatccgcgc aacgtcgttg atctgctgat ttacggcctg gcgggcgtca gctgctgcca
     1861 gttcacctat tttctgacga tccagctgtc cagtgcgggc atggggacga tcttgcagga
     1921 cctctcccct gtcattattc tgctggtggt ctgcatccag caaaggcgcg ggccgcgggt
     1981 gtttgagatt ttctccatcc tgctggcgct ggtgggggtg ttccttatca cgacccacgg
     2041 cagcctgacc gaccttgcca tcagcccggc ggcgctggcg tcgggcgttg tcagcgcctg
     2101 ctgcgtcgcc atctatacga tgtggcccaa gaaattgcag gagcagtacc ccaccccgat
     2161 gctgcagggc tgggcgttct tgatgggcgg cacgatgttt gccctgatct tccgcccctg
     2221 gacgataaat tatgtgccga cgccgatggg cgtgttcggt attttcacgg tcgtggtgct
     2281 gggcaatgtg ctggcgttca gcctgtatat gtcgggtgtg ccgctcatcg gcccgcagaa
     2341 gtccagcctg tacagctttg ctgagccggt cacggcggct attatcagca ccttggtgtt
     2401 gggctcgccg tttacgctgt gggatgcctt gggctttggc tgcatctttt tgatgctggt
     2461 gctgttgagt ctgccggcga aaacaaaagc gtaatagatt cgagaggctg tccgcaatgg
     2521 acagcctttt ttgctgccgt gtaggggccg gacatgtccg gccctcaacc tgcccgcgaa
     2581 cgaccgtccg cctttatgct gcgggccggg catgcccggc ccctacaacg ctataataaa
     2641 ataaacccat cctaactatg aattgacata tccccctccc tgggtgtatt atagtatcat
     2701 gtgtaaaact atatagccgc acctctacac gcggcggaaa atacagggga actgttatgt
     2761 ctttttttga aaaactgttc ggctctttct cggacaagga acttaagcgc atcaatccca
     2821 tcaaggacaa agtccttgcc ttggagccgg aaatggctaa gctgaccgac gaggaactgc
     2881 aggccaagac caccgaattc aaagagcgcc tggccaaggg cgagacgctg gacgatatcc
     2941 tgccggaggc gttcgccgtc tgccgtgagg ccgactggcg tgtgctgggc atgaagccct
     3001 atccggtgca gatcatcggc ggtattgtgc tgcaccgcgc ctgcatcgcc gagatgcaga
     3061 ccggtgaagg caagacgctg gttgccacca tgcctaccta cctgaacgcc ctgaccggcg
     3121 aaggcgtcca cgttgtcacc gtcaacgatt acctggcccg ccgcgactct gagtggatgg
     3181 gcaaggtcta ccgtttcctg ggtctgaccg ttggtctggt cgtccacggc gtggagtcca
     3241 ccgaccgcaa gaaggcctac gaggccgacg tcacctacgg cacgaacaac gagttcggct
     3301 tcgactacct gcgcgacaac atggtcgtct acaaggcgaa catggtccag cgcggccacg
     3361 cctttgccat tgtggacgag gtcgactcca tccttatcga cgaggcccgt accccgctga
     3421 tcatcagcgg caagggtgag gattccagcg tgatgtacaa gcgcgccgac gacttcgcca
     3481 agacgctgaa aaagagtgtc atcgtcgagt tggacgacaa ggttgaggcc gaggaacagg
     3541 tcgacggcga ttacgttgt
//
LOCUS       DBMDSQ010000211         3550 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_6021_length_3550_cov_1.812409, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000211 DBMDSQ010000000
VERSION     DBMDSQ010000211.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 3550)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3550)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3550
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6021_length_3550_cov_1.812409"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            74..505
                     /locus_tag="ACZPAX_07715"
                     /pseudo
     CDS             74..505
                     /locus_tag="ACZPAX_07715"
                     /inference="COORDINATES: protein motif:HMM:NF019217.6"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GLUG motif-containing protein"
     gene            536..1399
                     /locus_tag="ACZPAX_07720"
     CDS             536..1399
                     /locus_tag="ACZPAX_07720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113826.1"
                     /translation="MLGGIVGRSGYDSVAITNCYYLTGTAGQVYGDGKATVTNTTEKS
                     AFDFANGGVLALLMADRPADAQPWDTTCKYLESAGMTLPVLARQNLTVHDHVWSTYTT
                     DTAAKTHTHSCACGVVETEACTITPATCKDVSACAVCGQQYGGLDPHNHADLQHFPAV
                     AATTDAEGNKEYWYCGGCGKYFADAAAAREITQADTVTAKLPQPTTPPTATPTASPTA
                     QPTTAPQAAEQPRRTAQPTVQPTAAPTVQPVSTIPATGDTSSPILWAALLLCSGVGLA
                     VTAYKKNRHRA"
     gene            1557..2489
                     /locus_tag="ACZPAX_07725"
     CDS             1557..2489
                     /locus_tag="ACZPAX_07725"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005933392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HIR1113827.1"
                     /translation="MGIVVIGAVFVDIKGYPLSTYIPGGRNAGRVEQVHGGVSRNVAE
                     DIANVELRPTFVSLVDDTGSGQDVIDKLNKHKVNTRFIRRVHDGMGTWLAVFDNDGDV
                     TAAISKRPDTRPLTALLAENEEGIFADCDSVAIELDLEKETVKQVLRSAKKYGKRVYA
                     VISNMSIAMERRDFLQQIDCFVCNQQEAGMLFSDDYDHLAPAAMCRVLADNVRSARIP
                     CMVVTMGGQGAVYARANGECGIVPAKKVDVIDTTGAGDAFFAGTVIGLTYGKGLADSC
                     EIGSRLAASVICTAENVCPRFRPQEFGLDVEVVD"
     gene            2509..3504
                     /locus_tag="ACZPAX_07730"
     CDS             2509..3504
                     /locus_tag="ACZPAX_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007048708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR1113828.1"
                     /translation="MTIKDIARESGYAVGTVSRVLNHNPSVRPEAREKILAVVAQYGY
                     QPNANAKHLKQQAYEGLALVVKGTQNALFADLLDKLQAYVEKCGYTPTVYYINETGDE
                     MAYAQMLCTERKPYGIFFIGSHPHCFTPELAGLGIPCVLLTNNAASLGIPNLSSVSVD
                     DRAAAKVMIEHMYKQGRRRIGIIGDRPDCSRPSQQRLDGCLEAMQQLGLPFDFDKQYG
                     YARFSLEESYAAAEYLLAHCPDLDAVFAMSDLMALGAVRAFQDHGLRVPQDIGVAGYD
                     GISLGRFTVPRLTTIRQNTALLSQRAAQILLASIEHNAPAAHEIVPFELILGESV"
BASE COUNT          726 a         1107 c         1042 g          675 t
ORIGIN      
        1 ctttaacgcg aacaacgctg aacaagcggg ctgtattgca gcacttgcga aaagctcccg
       61 cattacgggc tgtgtgagca ccgcttctat aaatggacat cactcttttc agtcttcgac
      121 aggtggcatt gttggaattg cagattctag cacccaagta agtgactgta tcaattatgg
      181 cactgtaaag tgcacccctt ccaattatta cgctaccttt gcaggcggca ttgtcggatg
      241 tcttgcagat tattccaccg ttacaaattg cagtaacttc ggcaccataa tcggccttgt
      301 caacagcagt tccacccaaa cgatttatgt tggcggcatt gttggaactg ctcggcttga
      361 aagtatatcc gattgctaca acgctggtgc tgtaagaatc gatgaacaag gcaattcgtc
      421 cgccacgcaa ggggccggcg gcattgcagg atacgcggga gatgatgccc aatatctgac
      481 acatatttcc aactgttata acgttggcag tgtcacagct gccaatgcaa atgcaatgct
      541 gggcggtatt gtgggtaggt ctggctatga tagtgttgcc atcacaaact gctactacct
      601 gaccggcacc gccgggcagg tttacggcga tggcaaagcc accgtcacca acaccaccga
      661 aaaatctgcc tttgactttg ccaacggcgg cgtgctggcc ctgctgatgg cagaccgccc
      721 ggccgacgcc cagccgtggg acaccacctg caaatatctg gaatccgccg ggatgaccct
      781 gccggtattg gccaggcaaa acctgaccgt ccacgaccat gtgtggagca cctacacgac
      841 cgacactgcg gccaagaccc acacccacag ctgtgcctgc ggcgttgtgg agacggaagc
      901 ctgcaccatc accccggcca cctgcaagga tgtgtcggcc tgcgctgtct gcggtcagca
      961 gtacggcggc cttgaccccc acaaccacgc ggacttacag cacttccccg ctgtggcggc
     1021 caccacggac gccgagggca ataaggaata ctggtactgc ggcggctgcg gcaagtattt
     1081 tgccgatgcc gcagcggcca gggaaatcac ccaagccgac accgtgacgg caaagctgcc
     1141 ccagcccacc acgccgccga cggcaacccc gaccgcatcc cccacggcgc agcccaccac
     1201 cgcgccgcag gctgcggagc agccccgccg gaccgcacag cccacggtgc agcccacggc
     1261 agcccccacc gtgcagccgg tatccaccat ccccgccacc ggcgatacca gcagccccat
     1321 tttgtgggcc gccctgctgc tttgcagcgg cgtgggcctg gcagtaacgg catataagaa
     1381 aaacagacac cgtgcataaa aagtgtaggc agctgttcgt ggctgcctac attttttatg
     1441 ccgcatttgc aggggccgga tatgtccggc ctctgcgtgc cgtttttgta aaattcttgc
     1501 aatttttgcc gtaatacggt acactggttt tatagcaaac ggaaaggaaa cacaccatgg
     1561 gaatcgtagt aatcggcgca gtttttgttg acatcaaggg gtatcccctt tccacctata
     1621 tcccgggcgg gcgcaacgcc ggccgggtcg agcaagtcca cggcggcgtc agccgcaacg
     1681 tggccgagga tatcgccaat gtggagctgc gccccacctt cgtcagcctg gtggacgaca
     1741 ccggctccgg ccaggacgtc attgataagc tgaacaagca caaggtcaac acccggttca
     1801 tccgccgcgt gcatgacggc atgggcacct ggctggccgt gtttgacaat gacggcgacg
     1861 tgaccgccgc catctcgaag cggcccgaca cccgcccgct gaccgccctg ctggctgaga
     1921 acgaggaggg catctttgcc gactgcgaca gcgtcgccat tgagctggat ctggaaaaag
     1981 agacggtcaa gcaggtgctg cgctcggcca aaaagtacgg taagcgggtg tatgcggtca
     2041 tttccaacat gagcattgcg atggagcgcc gcgacttttt acagcagatc gactgttttg
     2101 tctgcaacca gcaggaggcg gggatgcttt tctccgacga ttacgaccac cttgcccccg
     2161 ccgccatgtg ccgcgtgctg gccgacaatg tgcgcagcgc ccgcatcccc tgcatggtgg
     2221 tgacgatggg cggccagggc gctgtctacg cccgcgccaa cggcgagtgc ggcattgtgc
     2281 ccgccaagaa ggtggatgtc atcgacacga ccggcgcggg cgacgcgttc tttgcgggca
     2341 cggtcattgg cctgacctac ggcaagggcc tggcggattc ctgcgaaatt ggcagccggt
     2401 tggcggcgtc ggtcatctgc accgccgaaa acgtctgccc ccgcttccgc ccgcaggagt
     2461 tcgggctgga cgtggaagtg gtggattgag gaaagaaggc agaccctttt gaccattaag
     2521 gatatcgccc gcgagtcggg ctacgcggtg gggaccgtct cccgcgtgtt gaaccataac
     2581 cccagcgtcc gccccgaggc acgggagaag atccttgccg tggtggcgca gtacggctac
     2641 cagcccaatg ccaacgccaa gcacctgaag cagcaggcct acgagggcct tgccctggtg
     2701 gtaaagggca cccagaacgc cctgtttgcg gatttgctgg acaaattgca ggcatatgtt
     2761 gaaaaatgcg gctacacgcc cacggtctat tacatcaacg agaccggcga cgagatggcc
     2821 tacgcccaga tgctctgcac cgagcgcaag ccctacggca tctttttcat cggctcgcac
     2881 ccgcactgct tcacgccgga gctggccggg ctgggcatcc cctgcgtgct gctgacgaac
     2941 aatgcggcga gcctgggcat cccgaacctg tccagcgtca gcgtggacga ccgcgccgcc
     3001 gcaaaggtca tgatcgagca tatgtacaag cagggccgcc gccgcatcgg catcattggc
     3061 gaccggcccg attgcagccg cccgagccaa caacgtctgg acggctgtct ggaggccatg
     3121 cagcagcttg gcctgccctt tgattttgac aagcagtacg gttacgcccg cttttcctta
     3181 gaggagagct acgcggcggc ggagtacctg ctggcccact gccccgatct ggacgccgtg
     3241 tttgccatgt cggatctgat ggcgctgggg gccgtgcgcg ccttccagga tcacggcctg
     3301 cgggtgccgc aggatatcgg cgtggccggg tacgacggta tttcgctggg gcggttcacc
     3361 gtgccgcggc tgacgaccat ccgccagaac accgcgctgc tctcccagcg tgccgcgcag
     3421 attttgcttg ccagcatcga gcataacgcc cctgcggcgc atgagattgt gccgtttgag
     3481 cttattttgg gggagagcgt gtgatacaaa ggctttcccc tttcccaata tcgcaccgta
     3541 ggggccggac
//
LOCUS       DBMDSQ010000212         3549 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_6023_length_3549_cov_1.926624, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000212 DBMDSQ010000000
VERSION     DBMDSQ010000212.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 3549)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3549)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3549
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6023_length_3549_cov_1.926624"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="ACZPAX_07735"
     CDS             complement(<1..213)
                     /locus_tag="ACZPAX_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015564642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HIR1113829.1"
                     /translation="MSRIGRKPIVIPAGVEVKVDAAEHAITVKGPKGTLHSKFHPLMN
                     VAVEGNEILVTRPNDEKEARSLHGLTR"
     gene            complement(230..628)
                     /gene="rpsH"
                     /locus_tag="ACZPAX_07740"
     CDS             complement(230..628)
                     /gene="rpsH"
                     /locus_tag="ACZPAX_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005934908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HIR1113830.1"
                     /translation="MQITDPIADLLTRIRNASTAKHPSVDIPASNLKKAICQILVDEG
                     YIKGMKVTEDNKQGTITLTLKYQENGTPVIAGLKRVSKPGLRIYTNCEDMPKVMKGLG
                     TAIISTSKGVMTDKAARAAHVGGEVLAFVW"
     gene            complement(695..880)
                     /locus_tag="ACZPAX_07745"
     CDS             complement(695..880)
                     /locus_tag="ACZPAX_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008752089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HIR1113831.1"
                     /translation="MAKLSMKLKQQQPAKFSTRAYNRCKICGRPHAYLRKYGVCRICF
                     RELAYKGEIPGVKKASW"
     gene            complement(896..1435)
                     /gene="rplE"
                     /locus_tag="ACZPAX_07750"
     CDS             complement(896..1435)
                     /gene="rplE"
                     /locus_tag="ACZPAX_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HIR1113832.1"
                     /translation="MARLKEQYVNEIAPALNKKFGYKSVMQIPKLDKVIINVAAGEAK
                     ENAKVIDAIVADLGQITGQKAIVCKARKSVANFKLREGMPIGAKVTLRGERMYEFVDR
                     LFNVALPRVRDFRGINGNSFDGRGNYAFGLKEQIIFPEIDFDKVDAIRGMDICFVTTA
                     KTDEEAKELLKALGAPFAN"
     gene            complement(1450..1764)
                     /gene="rplX"
                     /locus_tag="ACZPAX_07755"
     CDS             complement(1450..1764)
                     /gene="rplX"
                     /locus_tag="ACZPAX_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015537247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HIR1113833.1"
                     /translation="MNNLHVKTGDNVMVISGKDKGQTGKVLQTSPKEGKVIVEGLNMV
                     TKHVKPRRQGEQGGIVKAEGAIYACKVMPVCPKCGKATRVGHSVKDGKTVRVCRKCGA
                     EL"
     gene            complement(1780..2148)
                     /gene="rplN"
                     /locus_tag="ACZPAX_07760"
     CDS             complement(1780..2148)
                     /gene="rplN"
                     /locus_tag="ACZPAX_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011197180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HIR1113834.1"
                     /translation="MVQMQTYLKVADNTGAKELMCFRVLGGTRKRYANIGDIVVCSVK
                     KAAPGGTVKKGDVVKAVIVRSKHGLRRDDGSYIRFDENAAVIVMADKSPKGTRIFGPV
                     ARELRDAGFTKILSLAPESL"
     gene            complement(2182..2436)
                     /gene="rpsQ"
                     /locus_tag="ACZPAX_07765"
     CDS             complement(2182..2436)
                     /gene="rpsQ"
                     /locus_tag="ACZPAX_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005347536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HIR1113835.1"
                     /translation="MERNLRKTRVGVVVSDKMDKTIVVAVKDSVQHPLYKKILKRTKK
                     FKAHDENNEAGIGDRVEIMETRKISKDVNWRLVKIIEKAK"
     gene            complement(2452..2649)
                     /gene="rpmC"
                     /locus_tag="ACZPAX_07770"
     CDS             complement(2452..2649)
                     /gene="rpmC"
                     /locus_tag="ACZPAX_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HIR1113836.1"
                     /translation="MKATELREMTDVELNKQLKDLKAELFNLRFQHAINQLDNPIRIE
                     AVKKDIARVMTVLAEKNAKNA"
     gene            complement(2646..3086)
                     /gene="rplP"
                     /locus_tag="ACZPAX_07775"
     CDS             complement(2646..3086)
                     /gene="rplP"
                     /locus_tag="ACZPAX_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HIR1113837.1"
                     /translation="MLLPKRVKYRRVQRGRMTGKAMRGNKVNQGDYGLVALEPAWITS
                     NQIEAARVAMTRYIKRGGKVWIKIFPDKPVTEKPAGTRMGSGKGSPEYWVAVVKPGRV
                     LFELADVNEETAREALRLASHKLPIRCKFVKREELDTVKEGDAE"
     gene            complement(3086..>3549)
                     /gene="rpsC"
                     /locus_tag="ACZPAX_07780"
     CDS             complement(3086..>3549)
                     /gene="rpsC"
                     /locus_tag="ACZPAX_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015537246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HIR1113838.1"
                     /translation="VIGKGGAESAKIEKQLAALTGKAVNLNIIEVKGTTTNAQLVAED
                     VASQLERRIAFRRAMKQAIRNAMQPRDRSATPAKGIKVTCSGRLAGADIARVESYHEG
                     TIPLQTLRADIDYGFAEANTTYGKVGVKVWIYKGEILKGAKAPAKKEGGKQ"
BASE COUNT          658 a         1015 c         1021 g          855 t
ORIGIN      
        1 gcgggtcagg ccatggaggc tacgtgcctc cttctcgtca ttggggcggg taaccaaaat
       61 ctcattgccc tcgacagcaa cattcatcag aggatgaaat ttggaatgca gggtaccctt
      121 ggggccctta acggtgatag cgtgctcggc cgcatcgacc ttcacttcca caccggcggg
      181 aatgacgatg ggttttcttc caattctcga cattgtctat acccctttct taccacacaa
      241 aggcgagaac ttcaccgccg acatgagcag cgcgggctgc cttgtcggtc atgacgcctt
      301 tagaggtgga gatgattgcg gtgcccaggc ccttcatgac cttgggcata tcctcgcagt
      361 tggtgtaaat acgcaggcca ggcttagaaa cgcgcttcag gccggcgatg accggagtac
      421 cattctcctg gtacttcagg gtcagggtga tggtcccctg cttgttgtcc tcggtcactt
      481 tcataccctt gatgtagccc tcgtcaacca ggatctggca gatcgccttc ttcaggttag
      541 atgcggggat gtccacggaa gggtgtttgg cagtgctagc attgcggatg cgggtcagca
      601 ggtccgcgat aggatcggtg atttgcatgc cactaacctc cttaggttgt gtttaagagt
      661 gttgttattc gttcagacag aagcaatcaa gggcttacca ggaagccttc ttgacgccgg
      721 ggatctcgcc cttgtaagcc agctcacgga agcagatacg gcagacaccg tacttgcgca
      781 ggtaagcgtg gggacgaccg cagatcttgc agcggttgta agcgcgggtc gagaacttgg
      841 cgggctgctg ctgcttgagc ttcatagaaa gtttagccat ttacagttcc ctcccttagt
      901 ttgcgaacgg agcgcccaga gccttcagca gctccttggc ttcctcgtcg gtctttgcgg
      961 tggtgacaaa gcagatgtcc atgccgcgga tcgcgtcgac cttatcgaag tcgatctcag
     1021 ggaagatgat ctgctctttc aggccgaaag cgtagttgcc gcggccgtcg aagctgttgc
     1081 cgttgatgcc gcggaagtcg cggacacggg gcagcgccac attgaagagg cggtcgacga
     1141 actcgtacat acgctcacca cgcagggtga ccttagcgcc gatgggcata ccctcgcgca
     1201 gcttaaagtt tgcaacgctc ttgcgggcct tgcagacgat agccttctgg ccggtgatct
     1261 ggcccaggtc ggcgacgatg gcgtcgatga ccttggcgtt ctccttagcc tcaccagctg
     1321 caacgttgat gatgacctta tccagcttgg ggatctgcat aacgctcttg tagccgaact
     1381 tcttgttcag ggccggagcg atctcgttga catactgttc tttcaaacgt gccattgtgt
     1441 actcctccct tacagttcag cgccgcactt gcggcaaacg cggacggtct tgccgtcctt
     1501 gacagaatgg cccacgcggg tggccttgcc acacttgggg caaacgggca tgaccttgca
     1561 ggcgtagata gcgccctcgg ccttgacgat gccgccctgc tcaccctgac ggcgaggctt
     1621 gacgtgcttc gtgaccatgt tcaggccctc gacgatgacc ttgccctctt tcggggaggt
     1681 ctgcaggact ttgccggtct ggcccttgtc cttgccggag atgaccataa cgttgtcgcc
     1741 ggttttaaca tgaagattgt tcatttaatt aaaacctcct tacagcgatt ccggagccag
     1801 gctcaggatc ttggtgaagc cagcgtcgcg cagttcgcga gcgacaggtc caaagatacg
     1861 agtgcccttg gggctcttgt cggccatgac gataacggcg gcgttctcat caaaacggat
     1921 gtaggaaccg tcgtcacggc gcaggccgtg cttgctacgg acgatgacgg ccttgaccac
     1981 gtcgcccttc tttacagtac caccgggagc agccttcttg acgctgcaaa ccacgatgtc
     2041 accgatgttt gcgtatctct tgcgggtacc gcccagtaca cggaagcaca ttaactcctt
     2101 agcaccggta ttgtcggcca ctttgagata agtttgcatc tgaaccatct ttcaggtcct
     2161 cctttcaaag taccaggctg attattttgc cttctcgatg atcttgacca gacgccagtt
     2221 gacgtccttg gagatcttgc gggtctccat gatctcaacg cggtcgccga tgccggcttc
     2281 gttgttctcg tcatgggcct tgaacttctt ggtgcgcttc aagatcttct tgtacagagg
     2341 gtgctggacg ctgtccttaa cagcgacaac gatggtcttg tccatcttgt cggagacaac
     2401 gacgcccaca cgggtctttc tcagattacg ttccatgctt taccctccct cttacgcgtt
     2461 cttagcattc ttctcagcca ggacggtcat cacgcgggcg atgtccttct taacggcctc
     2521 gatgcgaatg gggttgtcca actggttgat ggcgtgctga aagcgcaggt tgaagagttc
     2581 ggccttcagg tccttgagct gcttgttcag ctcgacatcg gtcatttcgc gcagttcagt
     2641 ggctttcatt cagcgtcacc ctcctttaca gtgtcaagct cttcgcgctt gacgaatttg
     2701 cagcgaatcg gcagcttgtg gctggccaga cgcagagcct cgcgagcggt ctcctcgttg
     2761 acatcagcca gctcgaagag cacgcggccc ggcttaacga cggctaccca gtactccggg
     2821 ctgcctttac cggaacccat tcgggtgccg gccggcttct cggtaacggg cttgtcgggg
     2881 aagatcttga tccagacctt gccgccacgc ttgatataac gggtcatggc aacacgagct
     2941 gcctcgatct ggttggaggt gatccaagcc ggctcgagag cgaccaggcc gtaatcgccc
     3001 tggttgacct tgttgccgcg catggcctta ccggtcatgc ggccgcgctg gacgcggcgg
     3061 tacttaacac gcttcggcag taacattact gtttgcctcc ttccttcttg gcgggggctt
     3121 tggcgccctt caggatctcg cccttgtaga tccaaacctt gacgccgacc ttaccgtagg
     3181 tggtgttggc ctcagcgaag ccgtagtcga tgtcagcgcg cagagtctgc agggggatgg
     3241 taccctcatg gtagctctca acgcgggcga tgtcagcgcc agccagacgg ccggagcagg
     3301 tgaccttgat acccttggca ggcgtggcgc tgcggtcacg gggctgcatg gcattgcgga
     3361 tggcctgctt catggcgcgg cggaaagcga tgcggcgctc cagctgggaa gcgacgtcct
     3421 cagcaaccag ctgagcattg gtggtggtgc ctttgacctc gatgatgttg aggttgacag
     3481 ccttgccggt cagggcagcg agctgcttct cgatcttggc gctctcagca ccgcccttac
     3541 cgataacga
//
LOCUS       DBMDSQ010000213         3533 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_6053_length_3533_cov_2.093531, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000213 DBMDSQ010000000
VERSION     DBMDSQ010000213.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 3533)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3533)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3533
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6053_length_3533_cov_2.093531"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1217)
                     /gene="metG"
                     /locus_tag="ACZPAX_07785"
     CDS             complement(<1..1217)
                     /gene="metG"
                     /locus_tag="ACZPAX_07785"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014115915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HIR1113839.1"
                     /translation="MDQKKFYITTPIYYPSDKLHIGHTYCTVATDAMARYKRLQGYNV
                     KFLTGTDEHGQKIELKAKEAGVTPQQFVDNIVEGPKGVKDLWKLMNISYDRFIRTTDD
                     YHVAAIQKIFKKMYEKGDIYKGKYVGKYCTPCESFWTESQLVNGCCPDCGRPVVDAEE
                     EAYFFRLSKYADRVRDLLVNTDFLLPRSRVNEMVHNFIDPGLEDLCVSRTSFKWGIPV
                     DFDPKHVVYVWIDALFNYTTALGFMNDKYPDSDYETFWPADVHFIGKEIVRFHSIIWP
                     AMLMSMDMPLPKHVYGHGWLLLDGGKMSKSKGNVVDPYLLAERYSADALRYFLLRDFP
                     FGSDGNFSNELLINRINMDLANDLGNLLSRTTAMADKYFGGELPAEQAEGPEDAALIE
                     KATALRARYEADM"
     gene            complement(1447..2229)
                     /gene="cuyB"
                     /locus_tag="ACZPAX_07790"
     CDS             complement(1447..2229)
                     /gene="cuyB"
                     /locus_tag="ACZPAX_07790"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009323952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteate racemase"
                     /protein_id="HIR1113840.1"
                     /translation="MHNFAAGLIIYYKCYYKEGCATAQTKPQKQVLGILGGLGPAASC
                     YLYQMLIDHTPASCDQDHIDIVISSRASTPDRTAFIMGKSKDDPFAVMEQDGFSLVHY
                     GATVLAIPCNTAHYFYDRLAEALPVPVLNMPRLTVADAKAAGCTKLGILATDGTLAAE
                     TYQLACQAQDIDWAIPSEEHQQEVMSVIYDDIKQGKRADMAKFGAAVHDLKKQGCDMA
                     VLGCTELSLVKRDEHLGKFFIDSTEVLCRHALEACGVEPVGF"
     gene            2409..2933
                     /locus_tag="ACZPAX_07795"
     CDS             2409..2933
                     /locus_tag="ACZPAX_07795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113841.1"
                     /translation="MQPITYMTSLFTFYLKGEIKSEQNFISFKVPNTILGLIPLGAQT
                     EKFTISQIASTATNTRLSLKYLLLGILLVLWGFSGLTESFLLGLILLLLGINNVVNAF
                     IINLVVTTTAGQQKIISFLIFDKQKANQAAEQINAMIAGRLDDTNTRQQTDRVIDANA
                     QQTDRIIDAINSNK"
     gene            complement(3380..3454)
                     /locus_tag="ACZPAX_07800"
     tRNA            complement(3380..3454)
                     /locus_tag="ACZPAX_07800"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3419..3421),aa:Glu,seq:ctc)
BASE COUNT          716 a         1043 c          960 g          814 t
ORIGIN      
        1 tccatgtcgg cctcatagcg ggcgcgcagg gcggtggcct tctcgatcag ggcagcatcc
       61 tcggggcctt cggcctgctc ggcaggcagc tcgccgccaa agtacttgtc ggccatggcg
      121 gtggtgcggc tcaacaggtt gccgaggtca ttggccaggt ccatgttgat gcggttgatg
      181 agcagttcgt tggagaagtt gccgtccgaa ccgaacggga agtcgcgcag caggaagtag
      241 cgcagcgcgt cggcgctgta acgctcggcc agcaggtagg ggtcaaccac gttgcccttg
      301 gacttggaca ttttgccgcc gtccagcagc agccagccgt ggccgtaaac gtgcttgggc
      361 aagggcatat ccatactcat cagcatggca ggccagatga tggagtggaa gcggacgatt
      421 tccttgccga taaaatgcac gtcagcaggc cagaaggtct cgtaatcact gtcggggtac
      481 ttgtcattca tgaagcccag cgccgtggtg tagttgaaca gcgcgtcgat ccagacgtag
      541 acgacatgct tggggtcgaa atcgaccggg atgccccact tgaaactggt gcgggaaacg
      601 cacagatcct caaggcccgg atcgatgaag ttgtgtacca tctcgttgac gcgggagcgc
      661 ggcagcagga agtcggtgtt gaccagcagg tcgcgcacgc ggtctgcata cttggacaga
      721 cggaagaagt aagcctcctc ctcggcatcg acaacggggc ggccgcagtc ggggcagcag
      781 ccgttgacga gctggctctc ggtccagaag ctctcgcagg gggtgcagta cttgccgacg
      841 tacttgccct tataaatgtc gcccttctcg tacatctttt tgaagatttt ctggatggcg
      901 gcaacgtggt agtcgtcggt ggtgcggatg aaccggtcat agctgatgtt catgagcttc
      961 cacagatcct tgacgccctt ggggccttcg acgatgttgt cgacgaactg ctgcggggtg
     1021 acgcctgctt ctttggcttt cagctcaatc ttctggccgt gctcatcggt gccggtcagg
     1081 aacttgacgt tatagccctg caagcgctta tagcgtgcca tagcgtcggt ggcaacagtg
     1141 cagtaggtgt ggccgatatg cagcttatcg ctggggtaat agatcggcgt agtgatgtaa
     1201 aattttttct gatccatttg tgatttctct ccttatataa acaaaccgtt tttggtgtgt
     1261 tttttgcgct tatgctggat tttatattct gggcattcgg gcgaagcccg caaaagcctg
     1321 cccttacaag ggcgatggcc gagccgcgag ggcgaaaggg ctttgcccgt tcactgcagc
     1381 cacacgcgcc cgggcggttt cgccgcttcg tagggcggcc agtcctctag ccgcctcctc
     1441 gcgcctttag aaccccaccg gttccacccc gcacgcttcc agcgcgtggc ggcacagtac
     1501 ctcggtgctg tcaataaaaa acttgcccag gtgctcgtcc cgcttgacca ggctcaactc
     1561 ggtgcagccc aaaaccgcca tgtcgcaacc ctgctttttc aggtcgtgca ccgccgcgcc
     1621 gaactttgcc atatcggcac gcttgccctg cttgatgtcg tcgtaaatca ccgacatgac
     1681 ttcctgctgg tgttcctccg acggtatcgc ccagtcgatg tcctgcgcct ggcaggccag
     1741 ctggtaggtc tccgccgcca gcgtgccgtc cgtcgccagg atgcccagct ttgtgcagcc
     1801 cgccgccttg gcgtctgcca cggtcagccg gggcatattc agcaccggta ccggcagcgc
     1861 ctcagccagg cggtcgtaaa aatagtgcgc tgtgttgcag ggtatcgcca gcaccgtggc
     1921 accgtagtgt accaggctga aaccgtcctg ctccatcacg gcaaaggggt catccttgct
     1981 tttgcccatg atgaacgccg tgcggtcggg cgtcgatgcc cgcgacgaaa taacaatgtc
     2041 gatgtggtcc tgatcgcagc tggccggggt gtggtcgatc agcatttggt acaggtaaca
     2101 gctggccgca ggccccagcc cgcccagtat gcccagcacc tgtttttgtg gtttggtctg
     2161 cgccgtggca cacccctcct tataataaca cttatagtat attataaggc ctgcagcgaa
     2221 attatgcaag ggtgtattgc ccctgcgctg caaaaagaat cttgtgatag tatattgcat
     2281 tttctgacaa aatatgttat cctaacacca gaaaatgtag cttgttccgg cagtgctgcg
     2341 gcagggaaat tgcggctagt ttccttgcgg cggcaaccgg ttcaaaataa attatcggag
     2401 gaatttttat gcagcccatt acctacatga cttctctctt cacgttctac ctcaagggtg
     2461 aaatcaagag cgagcagaac tttatcagct ttaaagttcc caacacgatt ctcggtctga
     2521 ttcctctggg tgcccaaact gagaagttca ccatcagcca gattgcttcc accgctacca
     2581 acacccgcct gagcctgaag tatctgcttc tcggcattct tctggtgcta tggggatttt
     2641 cgggactgac cgaatctttc ctgctgggtt tgattctgct gctgcttggc atcaacaacg
     2701 ttgtcaacgc cttcatcatc aacctggtcg tcaccaccac ggctggtcag cagaagatca
     2761 tcagcttcct gattttcgac aagcagaagg ccaaccaggc cgctgagcag atcaacgcca
     2821 tgattgctgg ccgtttggac gacaccaaca cccgccagca gacggatcgc gtcatcgacg
     2881 ccaacgccca acagaccgac cgcatcatcg atgccatcaa cagcaacaag taaatccatc
     2941 gtatcatccc gcccatggcc ccgcgccatg ggcgtttttc tatttgtagg ggaggggttc
     3001 atccctcccg gcagcttcgc ctctaccagc acttaaactt ttcgcatccc agtaggggcc
     3061 gggcatgccc ggccctccac tgtatctcta tcgccccctt ccctttcacg ccgtaggggc
     3121 ggattccata tccgcccgcg caccttcgcc tttacgccct actaaccccc gcgttgcttt
     3181 actctacttt ctctacgttc gcgccgtgcg tggaatctcc cccgccctaa aaacagcccg
     3241 cagggctgtt tttgccccgc ggtacgctga cgcggggccc gggcttttcg attcctgcac
     3301 gggtcaccat ttacgcccga cacctattga aaagcaaata caaagaaaaa agcactacct
     3361 caaatgaagt agtgctttct ggtgacccgt gcgggaatcg aacccacgtt aacggcgtga
     3421 gaggccgctg tcttaaccgc ttgaccaacg ggccataata aaaagagaag ccgacatcta
     3481 cctattttca caggccgttt ccagccaact atcttcggca caagtgagct taa
//
LOCUS       DBMDSQ010000214         3528 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_6064_length_3528_cov_2.290341, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000214 DBMDSQ010000000
VERSION     DBMDSQ010000214.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 3528)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3528)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3528
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6064_length_3528_cov_2.290341"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            34..297
                     /locus_tag="ACZPAX_07805"
                     /pseudo
     CDS             34..297
                     /locus_tag="ACZPAX_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005926695.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
     gene            626..1822
                     /locus_tag="ACZPAX_07810"
     CDS             626..1822
                     /locus_tag="ACZPAX_07810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113842.1"
                     /translation="MTTHSITNRMPRLLAMLFLLLGVFCAGALTVHADDYDTTIDRLS
                     RLQALARDFSASQSDTPDPIELTLAYTRTGSYNTTIWQLTAGTRDPEFESYVSSNDPD
                     LVALQSVNTVTLPNGEKIDFGHLLASMNLVYNGIPITGSWGGDCQEMAKAYAGQASDA
                     AGYASAMRATFNIDDDGSTSYFGNQDLRADMDSVVVGSKVTKDTDLADALRSYYANLT
                     DYDRAREFISLSFGTVDTSSSDFADAVYRALMDDSGMQLLFYMNDMWSVQGWAIKDEY
                     APAVRGTTDLFAEYLSGAVNGEKIKSDTNDRLVAMGGQALSDALSALGDTDAAQAALS
                     AADSLANNAESTVSSGSDAISTARETFQTKFDVKIFQMILLIVGGLAAFMLVFSLVMF
                     AVRRRD"
     gene            2246..3316
                     /locus_tag="ACZPAX_07815"
     CDS             2246..3316
                     /locus_tag="ACZPAX_07815"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009323915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HIR1113843.1"
                     /translation="MLDIKITKTTHPKEKPQDESKLGFGKKFSDHMFLMDYTAGEGWH
                     DARIVPYGPWEMDPATTVFHYAQEIFEGMKAYRTAEGKIQLFRPECNANRFNDSADRL
                     GMPPIPPEDFVQAVKALVDVDRDWVPHSDGASLYIRPFCIATDVGLGVHAAKHYRFAI
                     ICAPSGAYYAEGLDPVRIYVEDEYIRAAPGLTGFTKCGGNYAASIKAGELAEERGFSQ
                     VLWLDGVEKKYVEEVGSMNIMFKIDGEIYTAPCVGTVLPGVTRRSIIELLKDWGYKVH
                     EERVAIADIMKAADEGKLEEVFGTGTAAVVSPVKELDWEGKVAHISGGKIGELTQKLY
                     DTLTGIQWGKLPDTKGWIVPVE"
BASE COUNT          806 a         1013 c          975 g          734 t
ORIGIN      
        1 tagacagcct ccatgccggt gcggatggag tacacgatct tcacgaccga gtactccatc
       61 cgcaccggca tggaggctgt ctataccctg ctggatgtag accgcggcgt gcccgaggtc
      121 tggggcagcg tctatgacat ccgctgcctg ctggacgcca ccgtcaagct gcgcgacggc
      181 cgcaagatca ccgacatgga gatgccgctg gtcggcaaga ttgccatgaa ggaagcgctg
      241 aaaaagattg aaggcaccga ggtggagaag ctgctgaagg aatacaacgt gatttaagcg
      301 gtcgacaatt tacaaattgt tcatacccat atcatagttt tttagcagtt atcctgtatc
      361 ttataagcac agggtcacgg agccgcaacc gatgatcctg gaacatgatt cctcctcaca
      421 caaaccgata ccccaagccc ccatccgaag ccgcaatcgg atgggggcga tttttgtgca
      481 cacagctttc acaaaaaaag tggtgacaaa ccaccgcccc ctgtggtata ctaaagatta
      541 caaattggtt aaaacaagat tttcctaccg gggcgcagat gcaaacgcgg tgtgccccgg
      601 ctttgccgtg tactaccgga ggtgcttgac tacgcattct atcacgaacc gtatgccccg
      661 cctgttggct atgctgtttt tgctgctggg ggtattttgt gcgggggcgc tgaccgtcca
      721 cgccgacgac tacgatacca ccatcgaccg cctttcccgc ctgcaggcgc tggcaaggga
      781 tttttcagca tcccagagcg acacgcccga ccccattgag ctgacgctgg cctacacccg
      841 caccggcagc tacaatacca ccatctggca gctgaccgcc ggtacccgcg acccggagtt
      901 tgagagctat gtcagcagca atgaccctga cttggtggct ttacagtctg taaacaccgt
      961 cacgctgccc aacggcgaga agatcgactt cgggcacctg ctggcttcga tgaaccttgt
     1021 ctacaacggc atccccatca ccggcagctg gggcggcgac tgccaggaaa tggccaaggc
     1081 ctacgctggc caggccagcg acgcagcggg ctatgccagc gccatgcgcg ccacctttaa
     1141 tattgacgac gacggctcga cgagttattt cggcaaccag gacctgcggg ccgacatgga
     1201 ctcggtggta gtcggctcca aggtgacgaa ggacactgac ctggccgacg cgctgcgcag
     1261 ctactacgcc aacctgaccg attacgaccg cgccagggag tttatctcgc tgagttttgg
     1321 cacggtggat acctccagca gtgactttgc cgacgccgta taccgcgccc tgatggacga
     1381 ttccggtatg cagctgctgt tctatatgaa cgatatgtgg tcggtgcagg gctgggccat
     1441 caaggacgaa tatgcccccg ctgtgcgcgg caccaccgac ctgtttgccg agtatctgtc
     1501 cggcgcggtc aacggcgaaa agatcaagag cgacacgaac gaccgcctag ttgccatggg
     1561 cgggcaggcg ctctccgatg cgctgtctgc cctgggcgac accgacgcag cccaggcagc
     1621 tttgagcgca gcggacagcc tggcgaacaa tgccgagtcg acggtgtcgt cgggttcgga
     1681 cgccatttcc accgcgcggg agaccttcca gacgaagttt gacgtgaaga ttttccagat
     1741 gattttgctg atcgtcggcg ggctggcagc gtttatgctg gtgttcagtc tggtcatgtt
     1801 cgctgtgcgg cgcagggatt gagatagcgt atagttttta gcgggcggct ttcccggaag
     1861 ggggaggccg cctttttgct gttcacatcc tattcacaat ccgtatattt gccacaactt
     1921 atccaccgcg tataggggtt atccaccgtg gaaatgttga aaactacgtg gaaactgtgg
     1981 aaaatcactc cactcgcagc cgttaaaaag cggaaaacct gaaaaatcca cgataatcaa
     2041 ccgtttactt tccacagttt caaccgtcac actgtggaaa acggtggaaa gccggggaaa
     2101 actgtccttg catttttcgc aacataaaat tcacaaaatg taaataaggg gttgcacttg
     2161 ccgctttaca gcgctataat aggcttaatc atcacccgca ctgctgtgcg gcggtgcgct
     2221 tgaatataaa gggagagtgc gaaaaatgtt ggatatcaaa atcaccaaaa caacccatcc
     2281 gaaagaaaaa ccgcaggacg agtccaagct cggctttggc aaaaagttca gcgaccatat
     2341 gttcctgatg gactataccg caggcgaagg ttggcatgat gcccgcatcg tcccctatgg
     2401 cccgtgggag atggacccgg ctaccaccgt gttccactat gcccaggaaa tttttgaagg
     2461 catgaaggcc taccgtaccg ccgagggcaa gatccagctg ttccgccccg agtgcaacgc
     2521 gaaccgtttc aatgattctg cggaccgtct gggcatgccc cccatcccgc cggaggattt
     2581 cgtccaggct gtcaaggcac tggtcgatgt tgaccgcgac tgggtgcccc attctgacgg
     2641 tgcatccctc tacatccgcc ccttctgcat cgctaccgat gtgggcctgg gcgtccacgc
     2701 cgccaagcat taccgctttg ccatcatctg cgcaccttcc ggtgcgtact acgccgaggg
     2761 tctggaccct gtccgcatct atgtcgagga tgagtacatc cgcgccgccc cgggcctgac
     2821 cggctttacc aagtgcggcg gcaactacgc tgcttccatc aaggctggtg agcttgctga
     2881 ggagcgcggc ttctcccagg tcctgtggct ggacggtgtc gagaagaagt acgtcgagga
     2941 agtcggctcc atgaacatca tgttcaagat cgacggcgag atttacaccg ctccctgcgt
     3001 gggtaccgtt ctgcccggcg tcacccgccg ctccatcatc gagctgctga aagactgggg
     3061 ctacaaagtc catgaggagc gcgtcgccat cgccgacatc atgaaggctg ccgacgaggg
     3121 caagctggag gaggtctttg gcaccggcac ggccgcggtc gtttcccctg tcaaggagct
     3181 ggactgggag ggcaaggttg cccacatcag cggcggcaag atcggcgagc tgacccagaa
     3241 actgtacgac accctgaccg gcatccagtg gggcaagctg cctgacacca agggctggat
     3301 cgttccggtt gaataatcca tattcagaaa caaattaaag aaactttctt gcgcttgcgg
     3361 tttacgatgc cgcaagcgct ttttctatga catttctatg aacgattgtg caatatgcac
     3421 aaaacagcca ccgtgcttta cagcacattg tacaagtacg catcttgcaa attcgacaaa
     3481 aaaggtgtac tataagagca tcgaaagcga gagcttccga gcgaaaag
//
LOCUS       DBMDSQ010000215         3527 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_6066_length_3527_cov_1.942207, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000215 DBMDSQ010000000
VERSION     DBMDSQ010000215.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 3527)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3527)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3527
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6066_length_3527_cov_1.942207"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="ACZPAX_07820"
     CDS             complement(<1..213)
                     /locus_tag="ACZPAX_07820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113844.1"
                     /translation="MYKKMAALLLAVALLCGCAAQVTVQRIPAELPKAESPAATPTPE
                     PTPTFTEEQKKYGSASLLSEPTVLISV"
     gene            complement(222..2537)
                     /locus_tag="ACZPAX_07825"
     CDS             complement(222..2537)
                     /locus_tag="ACZPAX_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015565224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HIR1113845.1"
                     /translation="MLCVKCQKRPAVVFVQRLENGKAIQEGYCLTCARAMHIQPVDDL
                     MKQFGMSDQDLESMEERMSSFLQEAADSGMMAPMMGGDMTDGGDENAPQDDFVPGGSP
                     VFPFGFGARQQKTDDAKGKNGKKDKAPRRKFLDTYCENLTQKARDGKTDRIIGRDREI
                     YRTIQILCRRQKNNPCLIGEAGVGKTAIAEGIAQRIAEGKVPARLQDKEIFLLDMTSL
                     VAGTQFRGQFEQRVKGLISEVKAAGNIILFIDEIHSIVGAGDSDGSMNAANIMKPALS
                     RGQMQVIGATTFAEYRKYIEKDSALERRFQPVKVEEPSLLDTIEVIKGIRVYYEKHHC
                     VRVPDPIVTSIVKLSERYITDRFLPDKAIDLLDEACACCNLRHPEITELIETQRTVAD
                     LEAREHELENPEPQNGQDAAIDYEALAQVKTDLAKQREKLPALQLKVAEIEVTMDDVA
                     QVIELWTGVPAVKIKETEYGRLQGLEDALKAHIIGQDEAVHAVAGAIKRARADLSGRH
                     RPASFIFVGPTGVGKTELVKQLANQLFDTVDPLISIDMSEYMEKYAVSRLIGSPPGYV
                     GYDEAGQLTEKVRRHPYSVVLFDEIEKAHPDVMNILLQILDEGKINDAQGRTVDFSNT
                     VICMTSNAGSTDTAGATGFGKKAEVASADRSMKALQEFLRPEFIGRVDEIITFHPLSE
                     ENLEKITALMLDEYKPGLAAHGITLHYGQPALADIVAESSTKYGARELRRTIRKTIED
                     PVSEALVDGRLDGREVTLELADHDGKAVLEI"
     gene            complement(2716..3387)
                     /locus_tag="ACZPAX_07830"
     CDS             complement(2716..3387)
                     /locus_tag="ACZPAX_07830"
                     /inference="COORDINATES: protein motif:HMM:NF014704.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HIR1113846.1"
                     /translation="MSFSPAILYDILFLIVFSFAAVKSWQKGFLAGLAELIGAVLGVG
                     IAVWGSRTLAPEIYQRFFSDAIANKVDQAVAQSGGDMAAAVQGLDFLPESLRTAVAAG
                     LQTAGDQLPEKITALLEPLFLPLVQVVLFVLLCVVVRWVFALLVRLLRGINGVPLVGG
                     VNRLLGLALGLCTGALDCWLLALVLWFLVSVTAGGLDWLNTAVLQRSVGYSFFGAFNP
                     FLVHY"
BASE COUNT          642 a         1066 c         1096 g          723 t
ORIGIN      
        1 gacactgatc agcaccgtcg gctcactcaa tagggacgcg ctgccgtatt ttttttgctc
       61 ctcggtaaag gtgggcgtcg gttccggcgt cggcgtggcg gcggggcttt cggccttcgg
      121 cagctcggcc gggatgcgct gcaccgtcac ttgggccgcg cagccgcaga gcagcgccac
      181 ggccagcagc agggcggcca tttttttgta catggcgggc ctcaaatttc cagaacggcc
      241 ttaccgtcgt ggtcggccag ttccagcgtg acctcgcggc catccagtcg gccatccacc
      301 agcgcctcgg agaccgggtc ctcgatggtc ttgcggatgg tgcggcgcag ttcacgcgca
      361 ccgtacttgg tggagctttc ggctacgatg tccgccaacg cgggctggcc gtagtgcagc
      421 gtgataccgt gggcggccag gccgggcttg tactcgtcca gcatcagcgc ggtgattttt
      481 tcaaggtttt cttcgctcag tgggtggaag gtgatgatct cgtccacgcg gccgatgaac
      541 tcggggcgca ggaactcctg cagcgccttc atgctgcggt ccgcgctggc gacctctgcc
      601 tttttgccaa agcctgttgc cccggctgtg tcggtggagc ctgcgttgga cgtcatgcag
      661 atgaccgtgt tggaaaagtc caccgtgcgg ccctgggcgt cgttgatctt gccctcgtcc
      721 aaaatttgca gcaagatatt catgacgtca ggatgcgctt tttcgatttc gtcaaacagc
      781 acgacgctgt acggatggcg gcgcaccttc tcggtcagct gcccggcctc gtcgtagccg
      841 acatagcccg gaggcgagcc gatcaggcgg gacaccgcgt acttttccat atactcgctc
      901 atgtcgatag agataagcgg gtcaaccgtg tcaaacagct ggtttgccag ctgcttgacc
      961 agctcggttt tgccgacgcc ggtggggccg acgaagatga aacttgccgg gcggtggcgg
     1021 ccggacaggt ccgcccgggc gcgtttgatg gccccggcca cggcatggac ggcctcgtcc
     1081 tggccgatga tgtgggcttt cagggcatct tccagccctt gcaggcggcc gtactcggtc
     1141 tccttgatct tcacggcagg cacaccggtc cacagctcaa tgacctgggc cacgtcgtcc
     1201 atcgtgactt caatttcagc cactttcagc tgcagggcgg ggagcttttc gcgctgcttc
     1261 gccaaatcgg tcttgacctg ggccagcgcc tcgtagtcga tggcggcgtc ctggccgttc
     1321 tgcggctcgg ggttttccag ctcgtgctcg cgggcttcca ggtcggccac ggtgcgctgc
     1381 gtctcgatca gttcggtgat ttcggggtgg cgcaggttgc agcaggcgca ggcctcgtcc
     1441 aacaggtcaa tggccttgtc gggcaggaaa cggtcggtga tgtaccgctc ggacagcttg
     1501 acgatgctcg tcacgatggg atccggcacg cggacgcaat ggtgcttctc gtagtaaacg
     1561 cggatgccct tgatgacctc gatggtgtcc agcagcgacg gctcctcgac cttgacgggc
     1621 tgaaaacggc gttccagcgc ggagtctttc tcgatatatt tgcggtactc ggcaaaggtc
     1681 gtcgcgccga tgacttgcat ctgcccgcgg gagagcgcgg gcttcatgat gttggcagca
     1741 ttcatcgagc cgtccgaatc accggcaccc acgatggagt gaatctcatc gatgaacaaa
     1801 atgatgttgc cggcggcctt gacctcgctg ataaggccct tgacgcgctg ttcaaactgg
     1861 ccgcggaact gggtgcctgc gacgagggag gtcatatcca gcaggaaaat ctccttgtcc
     1921 tgcaaacgcg cagggacttt gccctcggca atgcgctggg caatgccctc ggcaatggca
     1981 gtcttgccga caccggcctc gccgatgagg caggggttgt tcttctggcg gcggcaaaga
     2041 atttggatcg tgcggtaaat ttcgcggtcg cggccaatga tgcggtcggt tttgccgtcg
     2101 cgggccttct gggtcaagtt ctcgcagtag gtatccagga acttgcggcg gggagccttg
     2161 tccttcttgc cgtttttgcc cttggcgtcg tcagttttct gctggcgcgc accaaagccg
     2221 aagggaaaaa ccgggctgcc gccggggaca aaatcgtcct gcggggcgtt ctcgtcgccg
     2281 ccgtcggtca tatcgccgcc catcatgggg gccatcatgc cggagtcggc ggcctcctgc
     2341 aaaaagctgg acatacgctc ttccatgctc tccaggtcct ggtcggacat accgaactgt
     2401 ttcatcaggt catccaccgg ctggatgtgc atagcacgcg cgcaggtcag gcagtagccc
     2461 tcctggatgg ccttgccgtt ttccagccgc tgcacaaaga cgacggccgg gcgtttctgg
     2521 catttaacac aaagcatata cttcacctct atggaaagtg ccgcatgggc acgtgggatt
     2581 gctgaaaatt tgtggggcgt cctcgacgcc ctgcggcggc atatgggcat gaccaccccg
     2641 ccgtaccacc ttattactat tatcaagcaa accctgccaa aatgcaagtt ttggcggggt
     2701 ttgttcaaaa aatgtttaat aatggactaa aaacggattg aacgcgccaa aaaagctgta
     2761 cccgacgctg cgctgcaata cggcggtgtt cagccagtcc agcccgcccg cggtgacgct
     2821 gacgaggaac cagagcacca gagccagcag ccagcagtcc agcgcgccgg tgcacaagcc
     2881 cagtgccagg cccagcaggc ggttgacgcc gcctaccagc ggcacgccgt tgatgccgcg
     2941 cagcagacgt accagcaggg cgaacaccca ccgtaccacg acgcagagca gcacgaacag
     3001 aacgacctgc accagcggca ggaaaagcgg ctccagcagt gcggtgattt tttccggcag
     3061 ctggtcgcct gcggtttgca gcccggcggc cacggcagtg cgcaggcttt ccggtaaaaa
     3121 gtccagcccc tgcacggcag cggccatgtc cccgcccgac tgtgccacgg cctggtccac
     3181 cttgttggcg atggcgtcgc tgaagaagcg ctggtaaatt tccggggcca gcgtgcggct
     3241 gccccatacg gcaatgccca cgccaagcac cgcgccaatc agctcagcca gcccggcaag
     3301 aaatcccttc tgccagcttt tgaccgcggc gaaggaaaac acgataagga agagaatatc
     3361 atagagaata gcgggagaaa atgacataga tgggtacttc tttctgtgta aatgttttgg
     3421 ggtgcgaatg gcttccccca agggggaagg cttttagttc ggtgcctcca gcacgctggc
     3481 cctgctcccg gtaaacacca gcgtctgggc ggcgtccagc acggcca
//
LOCUS       DBMDSQ010000216         3521 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_6084_length_3521_cov_1.510526, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000216 DBMDSQ010000000
VERSION     DBMDSQ010000216.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 3521)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3521)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3521
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6084_length_3521_cov_1.510526"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..876
                     /locus_tag="ACZPAX_07835"
     CDS             <1..876
                     /locus_tag="ACZPAX_07835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113847.1"
                     /translation="GLESAEAAQAVVLSWVGGYAVCARLAGQLYDAGRISRRDAVRLQ
                     ILGCCSGPGFVIGCVGGLLLGNVRLGVVLYAAQIGANLAAGAVCLFGVCGRTRGNAPV
                     LRDGCGGRCASPPRGRMQASAPTVQQVSISTAITSAVTSSLSVCGCVVFFRLVGAVLG
                     AVIPLPSVLVSAALEVSAGCADFAVLGGQAALYGCCAVLSLLGVSVWAQMQLFAGAAF
                     CPQVLLASRLLHLVFLQGILRFCVRFLPGDVGVCSTLSARIVPLLHLPPDAAAVGFVF
                     LCAALYKAWQNLYNK"
     gene            909..1130
                     /locus_tag="ACZPAX_07840"
     CDS             909..1130
                     /locus_tag="ACZPAX_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006355383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113848.1"
                     /translation="MFKKTYYGANIAPACAYCLHGSTAADPKMILCKHKGVVSPYYCC
                     RKFSYDPLMRVPRRQVLPELDPRDFTLDD"
     gene            1143..2000
                     /locus_tag="ACZPAX_07845"
     CDS             1143..2000
                     /locus_tag="ACZPAX_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113849.1"
                     /translation="MDHPKFEALKKAWHEKRSVTMVYILLRTSVVLVMLAQIFNRNFE
                     NVFLCVLTLILFMLPSTLERKLDLDLPNTLEIIILLFIYAAEILGEIGAYYVTFPYWD
                     TVLHTLNGFLCAAIGFSLLDILNRHNDARFHLSPLYLAIMSFCFSMTVGVIWEFFECT
                     MDQLFFLDMQKDTVVSAIGSIMLDPTGGNTPIVLKNITDVIVVQADGTQTALGLGGYL
                     DIGLLDTMEDLFVNFIGALTFSIIGYFYVRSRGKGKFAKRFIPVANNSTPMAEESAPE
                     TEKDPGGQK"
     gene            1997..2539
                     /locus_tag="ACZPAX_07850"
     CDS             1997..2539
                     /locus_tag="ACZPAX_07850"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014118158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HIR1113850.1"
                     /translation="MISTCCYGSPLGDILLAADEVSLIGLWFEGQKYFASTLPAAHTV
                     QETPPLTQARRWLDVYFSGQQPDFTPPLHLVGSPFRQAVWTLLLQIPYGQTTTYGALA
                     RQLAAQTGAARVSAQAVGGAVGHNAVSLIIPCHRVVGTNGSLTGYAGGLDRKIKLLEL
                     EKADMRQLFVPCQEHRLIKL"
     gene            complement(2787..2859)
                     /locus_tag="ACZPAX_07855"
     tRNA            complement(2787..2859)
                     /locus_tag="ACZPAX_07855"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2824..2826),aa:Thr,seq:cgt)
     assembly_gap    2989..2998
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3038..>3521)
                     /locus_tag="ACZPAX_07860"
     CDS             complement(3038..>3521)
                     /locus_tag="ACZPAX_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046409.1"
                     /note="catalyzes the removal of amino acids from the N
                     termini of peptides; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HIR1113851.1"
                     /translation="GLNSMYTFHFLQQLCAGQGADYITACKAAKCLSADVTAAFDPTF
                     ADAFEPDNGTYAGCGVAIYKYTGSRGKSGTSDASAEMVSYLTGLMDKNNVIWQIGEMG
                     KLDLGGGGTVAKYVANQDIDTIDIGVPVISMHSPFEIVSKADVYMAYLTFKAFCEDAE
                     "
BASE COUNT          681 a          967 c         1037 g          826 t
ORIGIN      
        1 gggttagaat ctgcggaagc agcccaagcg gtcgtgctct cctgggtggg cgggtacgcc
       61 gtgtgcgcaa gattagccgg gcagctgtac gacgcgggga gaatttcccg ccgggacgct
      121 gtgcggctgc aaattttggg gtgctgctcc gggccggggt ttgtcattgg ctgtgtgggc
      181 gggctgctgc tgggcaatgt gcggctaggg gtcgtgctgt atgcggcaca gatcggggcg
      241 aatctggcgg cgggggcggt ttgccttttc ggggtgtgtg ggcgcacgcg cgggaacgca
      301 cctgttctgc gagatggctg cggggggcga tgcgcgtcac cgccccgcgg gcggatgcaa
      361 gcgtccgccc ctacagtgca acaggtcagc atttcaaccg ccatcacctc cgctgtcaca
      421 tcctcgctgt cggtctgcgg gtgcgtggtg tttttccggc tggtaggcgc ggtactgggg
      481 gcagtgattc cgctgccgtc ggtgttggtc agcgcggcgt tggaggtcag cgccgggtgt
      541 gcggactttg cggtgctggg cgggcaggcg gcgctgtacg gctgctgcgc agtgctcagc
      601 ctgctggggg tgtcggtctg ggcgcagatg cagctcttcg ccggggcagc gttctgtccg
      661 caggtgctgc tcgccagccg cctgctgcat cttgtatttt tgcagggcat tttacggttc
      721 tgcgtgcggt ttttgccggg ggatgtgggg gtgtgcagca cgctttctgc ccggatcgta
      781 ccgcttttgc atctgccgcc cgacgctgca gcggtggggt ttgtctttct ctgcgcagcc
      841 ctttacaagg cctggcaaaa cctttataat aaatagtggt atatttttgt attttgaggg
      901 tgaggctaat gtttaaaaag acctactatg gggccaatat cgccccagcg tgcgcctact
      961 gcctgcacgg ttccacggca gccgacccca aaatgattct atgcaagcac aagggcgttg
     1021 tttcgcccta ctactgctgc cgcaaattca gctatgaccc cttgatgcgg gtgccgcgcc
     1081 gccaagtctt gccggagctg gacccccggg attttacgct ggacgattaa gaaggagaaa
     1141 acatggacca cccgaagttc gaagccctca agaaagcgtg gcatgaaaaa cgttccgtca
     1201 cgatggtgta catcctgctg cgcacctcag tcgttttggt catgctggcg cagattttta
     1261 accgcaattt tgaaaacgta tttctctgcg ttctgacgct gattttgttc atgctgccca
     1321 gcaccttgga gcgcaagctg gaccttgacc tgcccaacac actggaaatc atcatactgc
     1381 tgtttattta tgcggcggaa atcctgggcg agatcggcgc gtactacgtg accttcccct
     1441 actgggacac agtcctgcac acgctgaacg gctttttgtg tgcggccatc gggttttccc
     1501 ttctggacat tttgaatcgg cacaacgacg cgcggttcca cctgtcgccc ttgtatctgg
     1561 ccattatgtc cttctgtttt tcgatgacag tgggcgtcat ctgggaattt tttgagtgta
     1621 cgatggatca gctgttcttc ctcgatatgc agaaggacac cgtcgtcagc gcgattggtt
     1681 cgatcatgct cgaccccacc ggcggcaaca cacccattgt gctgaaaaac atcaccgacg
     1741 tcatcgttgt gcaggctgac ggtacccaga ccgcgctggg cctgggcggg tatctggaca
     1801 ttggcctgct ggatacgatg gaagatttgt ttgtcaattt catcggcgca ctgacgtttt
     1861 ctatcatcgg ctacttctac gtgcgcagcc gcggcaaagg caagtttgcc aagcgtttta
     1921 tccccgtcgc caacaacagc acccctatgg cggaggagtc tgccccggaa accgagaaag
     1981 acccgggagg gcagaaatga tttccacctg ttgctatggc tccccactgg gcgacattct
     2041 tttagcggca gatgaagtca gcctgattgg gctgtggttt gaggggcaga aatattttgc
     2101 cagtaccctg cccgccgcac ataccgtgca ggaaacgccg ccactgaccc aggcacggcg
     2161 ctggctggat gtgtatttca gcggtcagca gccggatttt acaccgccgc tgcacctggt
     2221 cggctcccct ttccggcagg cagtgtggac gttgcttttg cagattccct acggccagac
     2281 gaccacctac ggcgcactgg cccggcagct tgcagcccag accggtgcgg cccgggtatc
     2341 cgcccaggcg gtgggcgggg ccgtgggcca caacgcggtc tccctcatca tcccctgcca
     2401 ccgcgttgtg ggcacaaacg gtagcctgac cggctacgcc ggaggccttg accgaaaaat
     2461 caagctactg gaactggaaa aggcggatat gcggcagttg tttgtcccct gccaggagca
     2521 ccgccttata aaactataaa tgcagcaaaa gccacagctc acccgccgtg gcttttgcta
     2581 ttgcacaatt tcgttgaaaa taaaatagat ttgcggtagc cacttttaat gcagattttg
     2641 tataagaata catacttcgc aaaggattgt ggaattttac tcacaaaaaa gattacccca
     2701 cagagagtta gcaatagatg ctatatctac gctattctct atggggtaat cctatgggtg
     2761 aaggcaaaat cttcaccctc attaaaagct ggtggccgga cttgaaccgg cgacctgctg
     2821 attacgaatc agctgctcta ccaactgagc cacaccagca cacaaggcaa cagtaaatat
     2881 tatacaggtt tttgcacaga agtttcagat ttttgtagtc cactactata taactttttc
     2941 cgtcgccgtc aacatttacg gcaagagctg caccgacatc atcgttgtnn nnnnnnnncg
     3001 gtaaaaaata tttctgctgc cctggtgtag gggctgctta ctcggcgtcc tcacagaagg
     3061 ctttgaaggt caggtaggcc atatatacat ccgccttgga gacgatctcg aacggagagt
     3121 gcatcgagat gaccggcacg ccgatatcga tggtgtcgat gtcctggttg gcaacgtact
     3181 tggcaaccgt gccgccgccg cccagatcca gcttgcccat ctcgccgatc tgccagatga
     3241 cgttattctt gtccatcaga ccggtcaggt agctgaccat ctcggcagag gcatcgctgg
     3301 tgccggactt gccgcgggag ccggtatact tgtagatggc cacgccgcag cccgcgtagg
     3361 tgccgttgtc cggttcaaag gcatcggcaa acgtcgggtc aaaggcggcc gtgacatcgg
     3421 cggacaggca cttggcggcc ttgcaggcgg tgatgtagtc tgccccctgc cctgcgcaga
     3481 gctgctgcag gaagtggaag gtgtacatac tgttcaggcc g
//
LOCUS       DBMDSQ010000217         3518 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_6093_length_3518_cov_1.925080, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000217 DBMDSQ010000000
VERSION     DBMDSQ010000217.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 3518)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3518)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3518
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6093_length_3518_cov_1.925080"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            142..1455
                     /locus_tag="ACZPAX_07865"
     CDS             142..1455
                     /locus_tag="ACZPAX_07865"
                     /inference="COORDINATES: protein motif:HMM:NF019561.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HIR1113852.1"
                     /translation="MANPNKPGVPGAKGAAPKGPRTIWDNPIFTMALAIILGFVTWTI
                     VTVFIDPQGSKIVANVPINYTSGASTYTAQGLDIVEKPDIEGVTVKVEGNSTIIGNIQ
                     NSDIMVYPSYAGVSGAGKVTLRLQARVTNTTDFPGDIDCTVESPETIDVVFDEVSEKT
                     IPVTVDASAVPISTGYMLNRTAAVPAEITLRGPTSELDQVSSIVAPVQIDGELADTTT
                     VPAMLELRDEEGNVFTPQYTSMDSDSANVTLTVYQVRELPLEVDFIGTPNGFDVESLH
                     CSLSQQTLKVAGPARTIGTLESLTVTDFDLAREFEPGRDYQRLIELPAGIVSLDGVTN
                     VTLEFDTADMASTKLNVSNIRAINVPSNYELQILSSLVSNVTLYGPAAEIEELSADSI
                     VAQIDCQSLNLTVGQQTIAVSIQIPSSSRIFATGSYTVQCEVTSK"
     gene            1535..3133
                     /locus_tag="ACZPAX_07870"
     CDS             1535..3133
                     /locus_tag="ACZPAX_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006520966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR1113853.1"
                     /translation="MLLVRNIRLPLTCPDPDAEAAAKALAILRVPARKAAHCGVAKLS
                     VDARHGTPVLVYTIAVTLNDEGEEPALAGASPCVCYTQPPKFDFSVGKIPLAHRPVVV
                     GLGPAGLFAALLLARNGYRPLVLERGPALDERIRAVGHFEKTGELNPNANIQFGEGGA
                     GTFSDGKLTTRVGDPLCAFVTGAFLDHGAPADIAWRQKPHVGTDLLRGVIRNIRGEIE
                     ALGGEVRFNTALTGLQTKNGALTGIETTVGSFPCEQLILAVGHSARDTFAMLHGLGLP
                     LECKPFSVGFRAEHLQTEIDKSLYHGAAGHPALPKGEYQLSQHVGGGRCVYTFCMCPG
                     GTVCAAASAEGGVVTNGMSLHARDGKNANAAVVVSVDGRDFDNDPAKAVAFQRRLEQA
                     AYRAGGGGYIAPAETVGSFLAGRGALELGAVQPTYPRGVTPCDLGSLLPGELSGALRD
                     GLTAFGRKLAAYRAPDAVLTGLETRTSSPIRLLRGKEDLQCTGLSGLYPCGEGAGYAG
                     GIMTAAVDGVRCAAELMKNWAPAQ"
     gene            3150..>3518
                     /locus_tag="ACZPAX_07875"
     CDS             3150..>3518
                     /locus_tag="ACZPAX_07875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113854.1"
                     /translation="MPDNKHNYEYNYDLGDVIDNALQMGSKIGSSVLGSIADALEQAG
                     DAMGMKQPDTFAAWKRRLERKWKNGGQSVGVVLTAVGWTFTGCFGITALVMGILTAVG
                     AGPLGLTHDEFMAIPILFGVF"
BASE COUNT          683 a         1122 c         1106 g          607 t
ORIGIN      
        1 agaacggtgt gctgatccgc cgcctggatc gccagaacct cttcaatctg ctgcaggagg
       61 agatcgtccc cccggcggag gacgaggccg aaaacacttc gcccctgcaa aagctgctgc
      121 gcaagggagg cgcactgaac catggcaaat ccgaataaac ccggcgtgcc gggggcaaag
      181 ggcgcagcgc ccaaaggccc gcgcacgatt tgggataacc cgatttttac aatggcgctg
      241 gccatcatcc tgggctttgt tacctggacg atcgttaccg tttttattga cccgcagggc
      301 agcaaaatcg tagcaaacgt gcccatcaac tacacaagcg gcgcgtccac ctacacggcc
      361 cagggcctgg atatcgtgga aaagccggac attgagggcg ttaccgtcaa ggtcgagggc
      421 aacagcacca tcatcggcaa catccagaac tccgatatca tggtctaccc cagctacgcg
      481 ggcgtcagcg gcgcgggcaa agtcacgctg cgcctacagg cccgtgtgac gaacactacc
      541 gatttccccg gcgatatcga ctgcacggtc gaaagccccg agaccatcga cgttgtgttt
      601 gacgaggtca gcgagaaaac catcccggtc acggtggatg cctctgctgt gccgatttcc
      661 accggctata tgttgaaccg caccgccgcc gtgcctgccg aaattacgct gcgcggcccg
      721 acgagcgagc tggatcaggt gtccagcatc gtggccccgg tgcagatcga tggcgagctg
      781 gccgacacga ccacagtgcc cgccatgctg gaactgcgcg acgaggaagg caatgtgttc
      841 acaccgcagt acacctcgat ggacagcgat tccgccaatg tgacgctgac ggtataccag
      901 gtgcgcgaac tgccgctgga ggtggatttc atcggcacgc cgaacggttt cgatgtagaa
      961 agcctgcact gttcgctgag ccagcagacg ctcaaagtgg ctggtccggc acgcaccatc
     1021 ggcacactgg aaagcctgac cgtgacggac tttgacctgg cgcgggagtt tgagccaggc
     1081 cgcgactacc agcggctcat cgagctgcct gcgggtatcg tctcgctgga cggcgtaacg
     1141 aacgttaccc tggaatttga taccgccgat atggcctcca ccaagctgaa cgtgtcgaac
     1201 atccgggcca tcaacgtgcc cagcaactac gagctgcaaa ttctgtcgag ccttgtcagc
     1261 aacgtgacgc tctacggccc ggcggctgaa atcgaggaat tgagcgccga cagcatcgtc
     1321 gcgcaaatcg actgccagag cttgaacctg accgtcggcc agcagaccat cgccgtcagc
     1381 atccagatcc cgtcgtccag ccgcatcttc gccaccggca gctacaccgt ccagtgcgag
     1441 gtcacgtcga aataacgcag tgccgtaggg gccgggtatg cccggcccct acccggtggc
     1501 gtgccataaa tcaataaccg aaggaatcct gtctatgctt ctagtccgca atatccgcct
     1561 gccgctgacc tgccccgacc cggatgccga ggcggcggcc aaggccctgg ccatcctgcg
     1621 tgtgcccgcc cgcaaggcgg cgcactgcgg cgtggcaaaa ctcagcgtgg acgcccggca
     1681 cggtacgccg gtgctcgtct acaccatcgc agtcacactc aacgacgagg gtgaggaacc
     1741 ggcgctggcc ggggcctcgc cctgtgtctg ttatacgcag ccgccaaaat ttgacttttc
     1801 tgtgggaaaa attccgctgg cgcaccgccc ggtcgtcgtg ggccttggcc ctgcgggcct
     1861 gtttgcggcg ctgctgctgg cccgcaatgg ctaccgcccg ctcgtgctgg aacgcggccc
     1921 tgcgctggat gagcgcatcc gggccgtcgg gcattttgaa aagaccggcg aactgaaccc
     1981 aaacgccaat atccagtttg gtgagggcgg cgcgggaacc ttttccgacg gcaagctgac
     2041 cacccgcgtc ggcgacccgc tctgcgcgtt cgtgaccggg gcgttcctcg accacggcgc
     2101 acccgctgat atcgcctggc ggcaaaagcc ccatgtgggt actgacctgc tgcgcggcgt
     2161 catccgcaat atccgcggtg aaattgaagc cttgggaggc gaggtgcggt tcaacaccgc
     2221 cctgaccggc ctgcagacga aaaacggtgc gctgaccggc attgagacaa cggtgggcag
     2281 tttcccctgc gagcagctga ttttggccgt cggccacagc gcccgggaca ccttcgccat
     2341 gctgcacggc ctgggtctgc cgctggaatg caagccgttc tccgtcggct tccgcgcgga
     2401 gcacctgcaa accgaaattg acaaaagcct ctaccacggc gcggcaggcc acccggcgct
     2461 gccaaagggc gagtaccagc tcagccagca cgtgggcggc gggcggtgtg tttacacctt
     2521 ctgtatgtgc ccgggcggca cggtctgcgc tgctgccagc gcggaaggag gcgtcgtcac
     2581 caacggtatg agcctgcacg cccgcgacgg caaaaatgcc aacgcggcgg tggtcgtcag
     2641 cgtggatggc cgcgactttg acaacgaccc ggccaaggct gtggcgttcc agcgccggtt
     2701 ggagcaggcg gcctaccgcg cgggcggtgg gggatacatc gccccggcgg agacggtcgg
     2761 cagctttttg gccgggcgcg gtgcgctgga attgggcgcg gtgcagccta cctacccgcg
     2821 cggcgtgacg ccctgcgatt tggggagcct gctgcccggc gagttgagcg gcgctctgcg
     2881 cgacggcctg acggcctttg gccgcaagct ggccgcttac cgcgcccccg acgcggtgtt
     2941 gacggggttg gagacccgca ccagcagccc aatccgcctg ctgcgcggca aagaggattt
     3001 acagtgtacc ggactttccg gcctgtaccc ctgcggagag ggcgcaggtt acgcgggcgg
     3061 catcatgacc gccgccgtgg acggcgtgcg ctgcgccgcc gaactgatga aaaattgggc
     3121 accggcccaa taaacagatt ggacaaacca tgccagacaa caaacacaat tacgaatata
     3181 actatgacct cggcgatgtg atcgacaacg cgcttcagat gggcagcaag atcggctcgt
     3241 cggtgctggg cagcattgcc gacgctctgg aacaggcggg ggacgcgatg ggaatgaaac
     3301 agcccgacac tttcgctgcc tggaaacgcc gcctggaacg caagtggaaa aacggcggcc
     3361 agtccgtcgg tgttgtgctg actgccgtag gctggacctt taccggctgc ttcggcatca
     3421 cggcgctggt catgggtatc ctgaccgctg ttggcgcggg cccgctgggc ctgacccacg
     3481 atgagtttat ggctatcccg attttgtttg gcgttttc
//
LOCUS       DBMDSQ010000218         3459 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_6205_length_3459_cov_2.169744, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000218 DBMDSQ010000000
VERSION     DBMDSQ010000218.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 3459)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3459)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3459
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6205_length_3459_cov_2.169744"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1039
                     /gene="aroB"
                     /locus_tag="ACZPAX_07880"
     CDS             <1..1039
                     /gene="aroB"
                     /locus_tag="ACZPAX_07880"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005921655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HIR1113855.1"
                     /translation="DVRSMKLTMQLKSRSYDIILKAGCLANLHQFTNVQGRKVFVLTD
                     SGVPADYAKIVAGQCQDSTVYTIPQGEGSKCLKVYGQVLQAMLEFGMDRKDLLVAVGG
                     GVVGDLGGFCAASYMRGIDFVNCPTTVLSQVDSSIGGKTAIDLGETKNIVGAFWQPKV
                     VLVDFDTLKTLPRRQIVNGLAEALKTGIIGDPQLFALFECEHPEENIEQIVYRSLKFK
                     KKIVEQDEREGSVRACLNFGHTIGHGIEAVRGVRGRRTTGLYHGECVALGMLPMIEDN
                     ALLKRTRAIYRTLGLPTRTGANKEKVLAYMQHDKKARGDSITIIKCPGLGCWRADKLP
                     MTELPTFLGID"
     gene            1097..2209
                     /gene="aroC"
                     /locus_tag="ACZPAX_07885"
     CDS             1097..2209
                     /gene="aroC"
                     /locus_tag="ACZPAX_07885"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HIR1113856.1"
                     /translation="MKNTFGNTVSMTIFGESHGPAVGAVLDGLAAGLPVDEAAIAAAM
                     DRRRARGDGLSTARTEADAVEFLSGVYNGHTTGTAITLMVRNLNTRSSDYAKTADLLR
                     PGHADYTAYAKYEGFQDARGGGHFSGRITAASVAAGTICETVLNSLGVKVYTHIAECA
                     GVQDAPLSSAAGLVMPDPQPGHFALLDASNEEAMQTAIRAAGSEGDSVGGILETIVTG
                     LPAGIGEPWFDSVESELAHLMFAIPACKGIEFGAGFGFAALRGSQANDPFTMRGDTVA
                     TATNKNGGINGGITNGMPIVFRTVLKPTPSIYKQQHTVDYIGKTDGELQIKGRHDPCI
                     VPRAAVVQNTLTAFGLLDLLTVRYGTLAQKHGLPQE"
     gene            2225..>3459
                     /locus_tag="ACZPAX_07890"
     CDS             2225..>3459
                     /locus_tag="ACZPAX_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HIR1113857.1"
                     /translation="MQQPLYGLIGGKLGHSYSKIIHEQIADYTYDLLPLPTEAEARAF
                     MEKRAFAAINVTIPYKQLVIPYCDEVDPKAKAIGAVNTIVNRDGKLYGYNTDYAGFAY
                     LAKAHGVDFADKTVLILGTGGTHSTVAAVCRDGGAKEILTASRTGKDGALLYSEAMHR
                     RDVQIIVNTTPCGMFPNVGQCLIDPKAFPALEAVLDVVYNPFRTELLLRAEDCGVTAA
                     GGFEMLVAQAVFAAEHFTGRQLDTAALIPAVSRRLRHELANVSIIGMPGCGKSTVGAA
                     LAKRLGKRFVDLDAEIERRTGHNIPDIFAQEGETAFRHYEAEVLAEVAKDNSQVIACG
                     GGVIKNPANTRALRQNGPVLWVRRPLERLATGGRPLSKGGAALKQLEAERTPLYEAAA
                     TAVLENSSTLTEAVNKAVEL"
BASE COUNT          701 a         1078 c         1071 g          609 t
ORIGIN      
        1 ggatgtgcga agcatgaaac taacgatgca gcttaaaagc cgcagctacg atattatttt
       61 gaaggccggc tgccttgcca acctccacca gtttaccaac gtgcagggcc gcaaggtctt
      121 tgtgctgacg gattccggcg tgcctgccga ctacgccaag atcgtggccg gccagtgcca
      181 ggactctacc gtctacacca tcccccaggg cgagggcagc aaatgcctga aagtctacgg
      241 ccaggtcttg caggccatgc tggaatttgg catggatcga aaagatttgc tggtcgccgt
      301 gggcggcggc gttgtaggtg atctgggcgg cttctgtgct gccagttata tgcgcggcat
      361 cgatttcgtc aactgcccca cgacggttct gtcccaggtg gactcctcca ttggcggcaa
      421 aacggccatc gacctgggcg aaacgaaaaa catcgtcggc gcgttctggc agccgaaggt
      481 cgtgctcgtc gattttgaca cactgaagac cctgccccgc cgccagatcg tcaacggcct
      541 ggccgaggcg ctgaaaaccg gcatcatcgg cgacccgcag ctgttcgccc tgtttgagtg
      601 cgagcacccc gaggaaaata ttgaacagat cgtctaccgc agtctgaagt tcaaaaagaa
      661 gatcgtcgaa caggacgagc gcgagggcag cgtccgcgcc tgcctgaact ttggccatac
      721 catcggccac ggcatcgagg ccgtgcgcgg tgtgcgcggc cgccgcacga ccgggctgta
      781 ccacggcgag tgcgtcgcgc tgggcatgct gccgatgatt gaggacaatg ctctgctcaa
      841 gcgtacccgg gcgatctacc gcacgctggg cctgcccacc cgcaccgggg ccaacaagga
      901 aaaagtcctg gcctacatgc agcacgacaa aaaggcccgg ggggattcca tcaccatcat
      961 caagtgcccc ggccttggct gctggcgcgc cgataagctg ccgatgaccg agctgccgac
     1021 tttcttgggc atagattgag tgtgcagggg cggatgcttg catcgccccc tgcggagata
     1081 tacaagagat aaacttatga aaaacacctt tggaaatacc gtcagtatga cgatttttgg
     1141 cgagagccat gggcctgcgg tgggggcggt gctggatggc ctggctgcgg ggctgcctgt
     1201 ggatgaggcc gccatcgctg cagccatgga tcgccgccgc gcccgggggg atgggctttc
     1261 caccgcccgc accgaggccg acgccgtgga atttctctcc ggcgtttaca acggccacac
     1321 gaccggcaca gccatcacct tgatggtccg caacctgaac acccgcagca gtgattacgc
     1381 caagactgct gacctgctgc gccccggcca cgccgactac acggcctacg ctaagtatga
     1441 aggctttcag gacgcacgag gcggcgggca tttctccggc cgcatcacgg cggccagcgt
     1501 cgcggcgggc acgatctgcg agaccgtgct gaacagcttg ggcgtaaaag tctacaccca
     1561 cattgccgag tgcgcaggcg tgcaggacgc gcccctttcc agcgcggcgg gactggtgat
     1621 gcccgacccg cagcccgggc attttgccct gctggatgcc tcaaatgagg aggccatgca
     1681 gacggccatc cgcgctgcgg gcagcgaggg tgacagtgtc ggcggcatcc tggagaccat
     1741 cgtcactggg ctgcccgccg gtatcggcga accatggttt gacagcgtcg aaagcgagct
     1801 ggcccacctg atgttcgcca tccccgcctg caaaggcatc gagtttggtg cgggcttcgg
     1861 ctttgccgcc ctgcgcggca gccaggccaa cgaccccttc acgatgcgcg gcgatacggt
     1921 tgccactgcc accaacaaga acggcggcat caacggcggc atcacgaacg gtatgcccat
     1981 cgtgttccgc acggtgctca aacccacgcc cagcatctat aaacaacagc ataccgtgga
     2041 ttatatcggc aagaccgacg gagaattgca gatcaagggc cgccatgacc cctgcattgt
     2101 gccccgtgcc gccgtggtgc agaacaccct gacggccttc gggctgctgg atcttttgac
     2161 cgtgcggtac ggcaccctgg cccaaaagca cggcctgccc caagagtaaa aggaagtgaa
     2221 cactatgcag caaccgctgt acggattgat cggcggcaag ctgggccaca gctactccaa
     2281 gatcatccat gagcagatcg cggattacac ctacgacctg ctccccctgc ccaccgaggc
     2341 agaagcccgc gcgtttatgg aaaaacgcgc gtttgcggcc atcaatgtga cgataccgta
     2401 taagcagctc gtcattccct actgtgacga ggttgaccct aaggcaaagg ccatcggtgc
     2461 ggtgaacacc attgtgaatc gggacggcaa gctgtacggt tacaacaccg actacgcggg
     2521 ctttgcttac ctggccaagg cccacggcgt ggattttgcc gataaaaccg tgctgattct
     2581 gggtacaggc ggtacgcatt cgacggtcgc agcggtctgc cgcgacggcg gcgcaaaaga
     2641 aattttgacc gccagccgca ccggtaagga cggtgcgctg ctgtacagtg aggccatgca
     2701 ccgccgcgat gtgcaaatca tcgtcaacac gacaccctgc gggatgttcc cgaatgtcgg
     2761 gcagtgcctg atcgacccga aagccttccc tgcactggaa gccgtgttgg atgtagtgta
     2821 caatcccttc cgcaccgaac tgctgctgcg ggccgaggac tgcggtgtga ccgcggctgg
     2881 cggctttgag atgctggtag cccaggccgt gtttgcggcc gagcatttca ccggcaggca
     2941 gctggatacg gcggccctca tccccgctgt cagccgccgt ctgcggcacg agctggcgaa
     3001 tgtctccatc atcgggatgc cgggctgcgg caaatccacg gtgggcgcgg cgctggccaa
     3061 gcggctgggc aagcgattcg tggatctgga cgcggaaatt gaacgccgca ccgggcataa
     3121 tattcccgac atctttgccc aggagggcga gactgctttc cgccactatg aggcggaagt
     3181 gctggccgag gttgccaagg acaacagcca ggtcatcgcc tgcggcggcg gggtcatcaa
     3241 aaatccggcc aacacccgcg ccctgcgcca gaacggcccg gtgctgtggg tgcgccgccc
     3301 gctggaacgc ctggctacgg gcgggcgtcc cctttcgaag ggcggggctg ccttaaagca
     3361 gctggaggcc gagcgcacac cgctgtacga ggccgccgcc accgccgttt tggagaatag
     3421 cagcaccttg accgaagccg tgaataaagc agtagaact
//
LOCUS       DBMDSQ010000219         3427 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_6275_length_3427_cov_1.959110, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000219 DBMDSQ010000000
VERSION     DBMDSQ010000219.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 3427)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3427)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3427
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6275_length_3427_cov_1.959110"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /locus_tag="ACZPAX_07895"
     CDS             <1..346
                     /locus_tag="ACZPAX_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009322406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HIR1113858.1"
                     /translation="NLSLGEYDHAPRQTAALDESIDTHRSFRDAHLTLTEDQVKIETA
                     RCLGCGASVVDPNKCIGCGVCTTKCEFDAIHLHRDLPECSKMVKSEDKFKAILPYMAK
                     REIKIRFSKKEK"
     gene            347..694
                     /locus_tag="ACZPAX_07900"
     CDS             347..694
                     /locus_tag="ACZPAX_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009322405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HIR1113859.1"
                     /translation="MHELGIVFHIIRTVEDVAKQNDVSRIRRVTLQLGEVSGVVESYL
                     QDCWKWAAAKSEILPGAVLAVETIPAVTLCEDCGQQYPTVQYGRICPHCRSEHTHLVQ
                     GNEMMIKEVEAVE"
     gene            complement(765..1310)
                     /locus_tag="ACZPAX_07905"
     CDS             complement(765..1310)
                     /locus_tag="ACZPAX_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006735322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HIR1113860.1"
                     /translation="MQFWNTFAAKHPAAAKWVREGGLFVIVSNLITVFKYLLLQFLPA
                     AFSSLPVVDFGWPGIPVTLFGETFQWNILGYDAAHGGLPYFCAYMIAMVIGECINFPI
                     QRNFVFRSKGSLGKQIAWYVAAFCIITCIVNSINCVWVAVAGLLVPDFIYNIGTTVLN
                     GGVSMVIFFFVNKVIFPEGEK"
     gene            complement(1325..>3427)
                     /locus_tag="ACZPAX_07910"
     CDS             complement(1325..>3427)
                     /locus_tag="ACZPAX_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015537136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HIR1113861.1"
                     /translation="GLNTKRSPLCGRNFEYFSEDPYLSGKMAAAYVRGIQSEGISACP
                     KHFAVNSQELRRMASDSVVDERTLRELYLTGFEIVVKEAHPKALMSSYNLINGIYANE
                     NAHLLQDILREEWGFDGAVVTDWGGSNDHALGVKNGSTLEMPAPGGDAVRELLAAVRS
                     GKIAEADVDARLDELLTLVLDTHAAVERHSRSFDADAHHALARRAAGESVVLLKNDDA
                     LLPLAEGTRVAVIGDFAETPRYQGAGSSAVNSIKVDTFLDCLNDSGLHSVGFAPGFDR
                     QGKPDAAKQAEAVALAAEADAVLLCLGLDEIKESEGLDRADMKLADNQIELLQAVREA
                     NPNTVVIVNAGASLETPWLAHCKALVYGALGGQAGAGAMVDVLTGKINPGGKLAETWA
                     NAYADTPARDHFAGPGRTVQYREGLYVGYRYYQTAGVPVAFPFGHGLSYTQFAYSDLH
                     ADAHSASLTVTNTGDRAGAEIVQLYVAKPNADIFRPAQELKAFAKVQLAAGESKTVTL
                     TLDDKAFRYWNTRTDSWEVEGGTYELRVGASSADIRLTAAVEVIGTDALNPYAGKALP
                     HYQSGQVQTVPDAEWETLLGRPIPDDRVKIDRNMTLGELNHGRSPLGWLVWAVLTVLL
                     NASYKKGKPDLNVLFQYNMPLRALAKMTNGAISMGMVDGIVMEVQGFWVIGLVRVIFE
                     AVKNVILNAQLENRLRNS"
BASE COUNT          664 a         1072 c         1050 g          641 t
ORIGIN      
        1 caatctgtcc ctgggcgagt acgaccatgc cccgcgccag accgccgcgc tggacgagag
       61 catcgacacc caccgttcct tccgggatgc gcacctgacg ctgaccgagg atcaggtcaa
      121 aatcgagaca gcccgctgtc tgggctgcgg cgccagcgtc gtcgacccga acaagtgcat
      181 cggctgcggc gtctgcacga ccaagtgcga gtttgacgct atccatctgc accgcgacct
      241 gcccgagtgc agcaagatgg tcaagagcga ggacaagttc aaggccattc tgccctacat
      301 ggcgaagcgg gaaatcaaga ttcgtttctc caaaaaggag aagtaaatgc acgagctggg
      361 cattgtgttc cacatcatcc gcacggtgga ggatgtggca aagcaaaacg atgtatcccg
      421 catccgacgg gtgacattgc agctgggcga agtctccggc gtggtggaga gctacctgca
      481 ggattgttgg aagtgggccg ccgcgaagtc tgagatttta cccggcgcgg tgctggcggt
      541 ggagaccatc cccgccgtga ctctctgcga ggactgcggg cagcagtacc ccaccgtgca
      601 gtatggccga atctgccccc actgccggag cgaacatacg catctggtgc aggggaacga
      661 gatgatgatc aaagaggtgg aggcagtaga ataaaagcct tcccccttgg gggaagccaa
      721 gaaaaggcgc gtgtgcgaga agttttcgca cacgcgcctt tttcttattt ctccccttcg
      781 gggaaaatca ccttattgac gaagaagaag atcaccatgg acacaccgcc gttcaaaacc
      841 gtcgtgccga tgttgtagat gaagtccggc accagcaggc cagccaccgc cacccaaacg
      901 cagttgatgg agttgacgat gcaggtgatg atgcagaacg ccgccacata ccaggcaatt
      961 tgcttgccca ggctgccctt gctgcggaac acaaaattgc gctggatcgg gaagttgatg
     1021 cactcgccaa tcaccatcgc gatcatgtag gcgcagaagt agggcagacc gccgtgggcg
     1081 gcatcgtagc caaggatgtt ccactggaaa gtctcgccaa agagcgttac cgggatgccc
     1141 ggccagccga aatccacgac cggcaggctg ctgaatgcgg cgggcagaaa ctgcaaaagc
     1201 aagtatttga acaccgtaat taagtttgac acgatgacga acaagccgcc ctctcgcacc
     1261 cacttggcag cggcggggtg cttagccgca aaggtattcc aaaattgcat ggctgcacct
     1321 cctgtcagct gttgcgcaga cggttttcca gctgcgcgtt caaaatcacg ttcttgacgg
     1381 cctcaaaaat cactcgcacc aggccgataa cccaaaagcc ctgcacttcc atcacgatac
     1441 cgtccaccat gcccatgctg atggcaccat tggtcatttt ggccagtgcg cgcagcggca
     1501 tattgtactg gaacagcaca ttcaaatccg gcttgccttt tttgtaactg gcgttcagca
     1561 gcacggtcag caccgcccag accagccagc ccagggggct gcgtccatgg ttcagctcac
     1621 ccagcgtcat gttgcggtca attttgaccc ggtcgtcagg gatggggcgg cccagcagag
     1681 tctcccactc ggcgtcgggt acggtttgca cctggccgct ctggtagtgc ggcagtgcct
     1741 tcccggcata ggggttcagc gcgtcggtgc cgatgacctc gaccgcagcg gtcaggcgga
     1801 tgtccgcaga gcttgcccct acgcgaagct catacgtgcc gccctccacc tcccagctgt
     1861 cggtgcgggt gttccagtag cggaacgcct tgtcgtccag cgtcagggtc acggtcttgc
     1921 tctcccccgc cgccaactgc accttggcaa aggctttcag ctcctgtgca gggcggaaaa
     1981 tgtcagcatt cggcttggcc acgtacagct gcacaatctc tgccccggcg cggtcgccgg
     2041 tgttggtcac ggtcaggctc gcgctgtggg cgtcggcatg caggtcactg tacgcaaact
     2101 gcgtatagct caacccatga ccgaagggga acgccaccgg caccccagcg gtttggtaat
     2161 agcggtagcc gacgtacagt ccctcgcggt actgcacggt gcggccaggg cctgcaaagt
     2221 ggtcgcgggc gggggtgtcg gcgtaggcgt tggcccaagt ctcggccagc ttgccgccgg
     2281 ggttgatttt gccggtcagc acgtccacca tcgcacctgc gcccgcctgg ccgcccagtg
     2341 cgccgtaaac cagcgctttg cagtgggcca gccacggcgt ttccagcgag gcaccggcgt
     2401 tcacaatgac caccgtgttg gggttggctt cgcgcacggc ctgcaaaagc tcgatctggt
     2461 tgtcggccag cttcatatcg gcgcggtcca agccctcgct ctccttgatc tcgtccagtc
     2521 ccaggcagag cagcacggcg tccgcttctg cggccagggc gacggcctcg gcctgcttgg
     2581 cggcatcggg tttgccctgg cggtcaaaac ccggggcaaa gcccacactg tgcaggccgc
     2641 tgtcgttcag acaatccaag aaggtgtcca ctttgatgga gttgacggcg ctggaccctg
     2701 caccctggta gcgcggcgtc tcggcaaagt cgccgatgac cgccacacgc gtgccctcgg
     2761 ccagcggcag cagggcatcg tcgtttttca gcagcacaac actctcccct gccgcgcggc
     2821 gggccagcgc atggtgggcg tcggcgtcaa aactgcggct gtggcgttct acggcggcgt
     2881 gggtgtccag caccagcgtc agcagctcat ccaggcgggc gtccacatcg gcctcggcga
     2941 ttttgccgga ccgtacggcg gccagcagct cccgcacggc gtccccgccg ggagcgggca
     3001 tttccagcgt cgagccgttt ttcacgccca gcgcatggtc attgctgccg ccccagtcgg
     3061 tcacgaccgc gccgtcaaag ccccactcct cgcgcaggat atcctgcaaa aggtgggcgt
     3121 tctcgttggc gtagatgccg ttgataagat tgtagctgga catgagggcc ttggggtgcg
     3181 cctctttgac gacgatctca aacccggtca ggtacagctc cctcagcgtc cgctcgtcca
     3241 ccaccgagtc cgatgccatg cggcgcagct cctggctgtt gacggcgaag tgcttggggc
     3301 aggcggagat gccttccgac tggatgccgc gcacgtaggc ggcggccatc ttgccggaga
     3361 gatagggatc ctcggagaaa tactcaaagt tgcggccgca gagggggctg cgcttggtgt
     3421 tcaggcc
//
LOCUS       DBMDSQ010000220         3402 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_6321_length_3402_cov_2.090579, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000220 DBMDSQ010000000
VERSION     DBMDSQ010000220.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 3402)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3402)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3402
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6321_length_3402_cov_2.090579"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1060)
                     /locus_tag="ACZPAX_07915"
     CDS             complement(<1..1060)
                     /locus_tag="ACZPAX_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003497180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HIR1113862.1"
                     /translation="MTPLEALKRYFGYDFFRPGQAEIVDAILAGRDALAIMPTGAGKS
                     ICYQVPALLLPGITLVISPLISLMQDQVKALNAAGIRATYINSSLSESQISRALNLAA
                     EGSYKIIYVAPERLESGAFRSFATAADISMVTVDEAHCISQWGQDFRPSYLKILDFVD
                     SLPERPILSAFTATATREVRDDIICTLRLHDPELLVTGFDRPNLYFQVEQTHRKDDFV
                     LQYLHDHPGESGIIYCATRKNVDKLQALLADYGFAATKYHAGLAAETRKKNQNDFIYD
                     KAPIIVATNAFGMGIDKSNVRFVLHYNMPQSMENYYQEAGRAGRDGLPSQCVLLFSAQ
                     DVVINKFLLDRKDFSESDD"
     gene            complement(1300..2199)
                     /locus_tag="ACZPAX_07920"
     CDS             complement(1300..2199)
                     /locus_tag="ACZPAX_07920"
                     /inference="COORDINATES: protein motif:HMM:NF024242.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HIR1113863.1"
                     /translation="MIIQKVPHYVDSVLTQEVASAALQGGDVVVAWYTNQVNSQSVVH
                     SHPYYELILPVAGSSVRYSVGGSVYDLHLGELILFPGEMYHSCKFNITADTSERLVVQ
                     IAPGIWDRAWQQSGLPRRAWNRDPIILDANAVLHWDFRGLLERMAQTVSLDERIRPIV
                     QCCEVTELILLASHVITRRNAAPPPSATSLLIAKAVDYLQTHYTDPGLTVAQLAKYTY
                     TSREHLSRVFKEYTLESVHGYLTNLRMQHCRNAIAAGASVLDACNASGFTNYSSFLKS
                     FRALYGVTPTEYRAQLRQSLAPN"
     gene            2415..>3402
                     /locus_tag="ACZPAX_07925"
     CDS             2415..>3402
                     /locus_tag="ACZPAX_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007045616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM-barrel domain-containing protein"
                     /protein_id="HIR1113864.1"
                     /translation="MKVCYTGGAVTDHGSYYAVATNAVELRIWFLTDDILRIRAGFDG
                     DWDEASYSLTMTAWDSRTDELMKDCRKRVQPAEAALTDGGKQAVIQGKRLKVVIEKAP
                     FRIMVYDKDGSLLHADIPDLAYREDSNHRRIHASQIEADDCFYGFGEKSGEINKAEKY
                     MNMAPGDAMGYNAKETDSLYKHIPFYIRLNRGTRQAVGYFYHNTAECDFNMGREKRNY
                     WHRYSSYRTDAGDIDLFLIAGPSIREVIERYTDLTGKSVMLPKAALGYLGSSMYYPEL
                     PENSDDAVLDFIDTTREEGIPADGFQLSSGYCAVETPEGIQLNSSTCNSFPPN"
BASE COUNT          714 a          995 c          998 g          695 t
ORIGIN      
        1 cgtcgtcgga ttccgaaaaa tccttgcggt ccagcaaaaa cttgttgatg accacatcct
       61 gtgccgaaaa gagcaaaacg cactgggacg gaagtccatc gcgcccggcg cggcctgcct
      121 cctggtaata attctccatg ctttggggca tattgtagtg cagcacgaac cgcacgttgg
      181 atttgtcgat gcccatgccg aaggcgttcg tcgccacaat gatgggggct ttgtcgtaga
      241 tgaaatcgtt ctggttcttc ttgcgcgtct cggcggccag acccgcgtgg tacttggtgg
      301 cggcaaagcc gtagtcggcc agcagggctt gcagcttgtc cacatttttg cgggtggcac
      361 agtagatgat gccgctctcg cccgggtggt cgtgcaggta ttgcagcaca aaatcatcct
      421 tgcggtgggt ctgctccacc tgaaagtaga ggttcggccg gtcaaaaccg gtgacaagca
      481 gctccgggtc atgcagccgc agcgtgcaga tgatatcgtc ccgcacctcc cgcgtggcag
      541 tggccgtaaa ggcggacagg atggggcgtt ccgggaggct gtccacaaaa tcaaggattt
      601 ttaagtagct cgggcggaaa tcctggcccc actggctgat gcagtgggcc tcgtccactg
      661 tgaccatcga gatatccgcc gccgtagcaa agctgcggaa cgcgccgctt tccagccgct
      721 cgggcgcaac gtagatgatt ttatagctgc cctcggcggc caggttcagc gcacggctga
      781 tttggctttc tgacagggaa gaattgatgt atgtcgctcg gatgccagcc gcgttcagcg
      841 ccttgacctg gtcctgcatc aaggagatca gcggcgagat gaccagcgtg atgcccggca
      901 gcagcagcgc cgggacctgg tagcagatag atttgcccgc gccggtgggc atgatagcca
      961 gcgcgtcccg cccggccagg atggcgtcca caatttcagc ctgcccgggg cggaagaaat
     1021 cataaccgaa atagcgtttc agcgcctcca acggtgtcat gcagtgtgcc cccctcttta
     1081 cagtagtatt ctaaggcaat accgcacaat tcccgcctga cggcttaagc accgctccgg
     1141 cggctgcggc acggcatctg cgttgccaaa atgctcgata agacatccag tattatctgc
     1201 gcttttggct tagcagctgc cgcacctcgc tcgctgtatc ggcacttaga attatgcggt
     1261 attgccctaa gtataccccg atttgccgcc agtgtaaagc tagttagggg ccaggctttg
     1321 ccgcagctgg gcgcggtact cggttggggt aacgccgtac agcgcgcgga aactctttaa
     1381 aaaactgctg taatttgtaa agccgctggc attgcaggcg tccagcacgc tggccccggc
     1441 cgcaatggcg ttgcggcagt gctgcatccg caggttggtc aggtagccgt gtacgctttc
     1501 cagcgtgtac tccttgaaca cgcgggacag atgctcccgg cttgtgtagg tgtacttggc
     1561 cagctgtgcc acggtcagcc cggggtcggt gtagtgggtt tgcaggtagt caacggcctt
     1621 ggcaatcagc agactggtgg ccgagggcgg cggtgcagcg ttgcgccggg tgatgacatg
     1681 gctggcgagc agaatcagct ctgttacctc gcagcattgc acgatggggc gtatcctttc
     1741 gtccagtgag acagtctgtg ccatccgttc cagcaggccg cggaaatccc agtgcaggac
     1801 ggcgtttgca tccaagatga tggggtcgcg attccatgcg cggcggggca gaccgctctg
     1861 ctgccaggca cggtcccaga tgcccggtgc gatctgcacg acgagccgct ccgaagtatc
     1921 cgccgtgatg ttgaacttgc aggagtggta catctcgccg gggaacaaaa tcagttcgcc
     1981 caggtgtaaa tcgtagacgc tgccgccgac ggaatagcgg acactgctgc ccgccaccgg
     2041 caaaatcagc tcatagtagg ggtgggagtg gacgaccgac tggctgttga cctggtttgt
     2101 gtaccaggct accactacgt caccgccctg cagggcggcg ctggcgacct cctgtgtcag
     2161 aacagaatct acatagtgcg gaactttttg gataatcatg gcgtcggccc tcctgcaatt
     2221 tttgtcctta aaacgattat atcacatttt gcaacattcg tgccacaaat gcagaagaaa
     2281 tttcatgaac aatttgttat tataaggtcg atacagggcg aataacttat agatgcacaa
     2341 aaattatatc cgatttttgt gcatattgcc tatttgtgcc cgaactaccg catcacaaag
     2401 aaaggggaaa atctatgaaa gtgtgctaca ccggtggcgc agtcaccgac cacggcagct
     2461 actatgctgt ggctactaac gccgtggaac tgcgcatctg gttcctgacc gacgacatcc
     2521 tgcgcatccg cgcgggcttt gacggcgatt gggacgaagc ctcttacagc ctgaccatga
     2581 cggcgtggga ctcccgcacc gacgaactga tgaaggactg ccgcaagcgc gtccagcccg
     2641 ccgaggctgc gctgaccgac ggcggcaagc aggccgtcat ccagggcaag cggctgaaag
     2701 tcgtcatcga gaaagcgccg ttccgcatca tggtctatga taaggacggg agcctgctgc
     2761 acgccgatat tcccgacttg gcctaccgcg aggacagcaa ccaccgccgc atccatgcca
     2821 gtcagatcga ggctgacgac tgcttctacg gctttggcga gaaatccggc gagatcaaca
     2881 aggccgagaa atacatgaac atggcccccg gcgacgccat gggctacaac gccaaggaga
     2941 ccgactccct ctacaagcat attccgttct acatccgcct gaaccgcggc actaggcagg
     3001 ctgtgggcta tttctaccac aacacggccg agtgtgattt caacatgggc cgcgagaagc
     3061 gcaactactg gcaccgctat tcttcctacc gcaccgacgc gggcgacatt gacctgttcc
     3121 tgattgccgg cccgtccatc cgcgaggtca tcgagcgcta caccgacctg accggcaaga
     3181 gcgtcatgct gccgaaggcc gcgctgggct atctgggcag ctcgatgtat tatcccgagc
     3241 tgcccgagaa cagcgatgac gccgtgctcg acttcatcga cacgacccgc gaggagggca
     3301 tccccgcaga cggcttccag ctgtccagcg gctactgcgc ggtcgagacg cccgagggca
     3361 tccagttgaa ctcctccacc tgcaacagct tcccgccaaa ct
//
LOCUS       DBMDSQ010000221         3396 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_6331_length_3396_cov_2.752352, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000221 DBMDSQ010000000
VERSION     DBMDSQ010000221.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 3396)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3396)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3396
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6331_length_3396_cov_2.752352"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(53..1036)
                     /locus_tag="ACZPAX_07930"
     CDS             complement(53..1036)
                     /locus_tag="ACZPAX_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008401494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HIR1113865.1"
                     /translation="MNNRKRTALCLLGAALCVFMTFGAALALGRYPITPAALLAGDAM
                     AVRTFTVLRLPRAVMALIGGFGLGAAGFVYQTVFRNPLASPDIIGVSSGASVGAAFAI
                     LFVSSGALSTTVCAFAGGLAAVFLSLGLAAVAPGRSKMSLVLAGIAVHALAQTLLMLL
                     KLTADPEKELASIEYWIMGSLAAVTRSRVWFPVPVVLVCCAAIFALHRQALLLSIEEG
                     EARLLGVRVGAMRLLLLTLATMTVAAVVSVTGLISFVGLLAPHSARLLAGHNRRSACL
                     LSGLLGSALLLAADTLAKTAASTELPVSIFTSLLGVPFLLYLILRPGRDAS"
     gene            complement(1029..2120)
                     /locus_tag="ACZPAX_07935"
     CDS             complement(1029..2120)
                     /locus_tag="ACZPAX_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015553262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR1113866.1"
                     /translation="MKMTKMTALLLAAAMALTACGSTAAVSSEAAASATAAPEVTASP
                     EEATAAPAAAETAVTYPLTVTDQLGREVTIETEPKTLASGYYISTSLLIALGVQDELV
                     GVEAKADKRTIYSLSAPELQSLPSIGTAKEFDLEGCAALTPDLVIVPAKLKDSIPQME
                     ELGLTVLAVKPEDQDKLYGAIDLLAQATNTVARGEELKSAIEDNLLSLHEAIGDAEAP
                     TVYMAGNSSVLQTAGPAMYQNYMIENAGGLNAAASVEDTYWAEVSYEQVLDWDPDYII
                     LASDADYDVDSVLNDAALADCTAVREGHVYQLPHAIEAVDSPVPGSFLGSVYLGSVLH
                     PEQVSEQFYHDCADAFYTTYYGFTPASYE"
     gene            2267..3028
                     /locus_tag="ACZPAX_07940"
     CDS             2267..3028
                     /locus_tag="ACZPAX_07940"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015564051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HIR1113867.1"
                     /translation="MMTIREYARPATLEEAWQLNQKKPNRVLGGMLWLKMEKINVGTA
                     IDLSALGLDTIEETDTEIIIGAMATLRDLETNAALNAYTAGAVREAVRHIVGVQFRNC
                     ATVGGSIYGRFGFSDVLTLFLALDCEVELYRAGRMPLAQFAAMPYDRDILTHICLRKT
                     PGLKVQYQSVRATQTDFPILTCAAARTAQGQYRFSIGARPMKAVLVTPDCDPALLPAT
                     VQAAVKTGSNLRGSAEYRTHLTGVLVRRAVAALEN"
     gene            3041..>3396
                     /locus_tag="ACZPAX_07945"
     CDS             3041..>3396
                     /locus_tag="ACZPAX_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015538514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HIR1113868.1"
                     /translation="MQITFTLNHKKVTAEIAADTVLLDLVRSLGCKSVKRGCETANCG
                     LCTVFLDEKPVLSCSVLAARVNGRSVTTLEGLQEEAAEFGAFIADQGAEQCGFCNPGF
                     IMNALALFRENPDPGE"
BASE COUNT          641 a         1056 c         1144 g          555 t
ORIGIN      
        1 acggtgaggt cctgcacgca aagccccacg ggcgggggcg tggtttgtgg catcatgacg
       61 catccctccc cggccgcagg atcagataaa gcagaaacgg tacgcccagc agcgaggtaa
      121 agatgctgac cggcagctca gtggaggcag ccgtcttggc cagcgtgtcg gcggccagca
      181 gcagggcgct gcccagcagc ccgctcagca ggcaggccga gcgccggttg tgccccgcca
      241 gcagccgggc gctgtggggg gccagcagcc cgacaaagct gataagccct gtcacgctga
      301 caacggcggc aaccgtcatg gtggcaagcg tcagcagcag caggcgcatc gcacccaccc
      361 gcacgccgag cagccgggcc tcgccctcct cgatcgagag cagcagcgcc tgccggtgca
      421 gcgcaaagat cgctgcgcag cagaccagca cgaccggcac cgggaaccac acccggctgc
      481 gggtcacggc ggccagactg cccatgatcc aatactcgat gctggccagc tccttctcgg
      541 ggtcggcggt cagcttgagc agcatcagca gcgtctgggc cagcgcgtgg acggcgatgc
      601 ccgccagcac cagcgacatt ttgctgcgcc ccggcgcgac agcggccagc cccagcgaga
      661 ggaacaccgc cgccagcccg cccgcaaagg cacagaccgt ggtggacagc gcccctgacg
      721 aaacgaacag gatggcgaac gccgcgccga cactggcccc cgaggaaacg ccgatgatgt
      781 cggggcttgc cagcgggttg cggaatacgg tctggtagac aaagccggcc gcccccagcc
      841 caaagccgcc gatcagagcc ataacggcgc ggggcagccg cagcacggtg aaggtgcgca
      901 ccgccatggc atcgccggcc agcagcgccg ccggtgttat ggggtagcgg cccagcgcaa
      961 gggccgcacc aaaagtcata aaaacacaga gcgctgcgcc cagaaggcac agcgccgtgc
     1021 gcttgcggtt attcatagga ggcaggggtg aagccgtagt aggtcgtgta gaatgcatcg
     1081 gcgcagtcat ggtagaactg ctcgctcacc tgctcggggt gcagcacgct gcccagatag
     1141 acgctgccca ggaagctgcc gggcacgggg gagtccacag cctcgatggc gtggggcagc
     1201 tggtagacat ggccctccct gacggcggtg cagtcggcca gtgcggcatc gttcaggacg
     1261 gaatcgacat catagtcggc atcggacgcc agaatgatgt agtcgggatc ccagtccagc
     1321 acctgctcat aggagacctc ggcccagtag gtgtcctcaa cactggcggc agcgttgaga
     1381 ccgccggcgt tctcgatcat atagttctgg tacatggcgg ggccggcggt ctgcagcaca
     1441 gagctgttgc cggccatgta gacggtgggg gcctcggcat cgccgatggc ctcgtgcagg
     1501 ctgagcaggt tgtcctcgat cgcggacttc agctcctcac cgcgggcaac ggtgttggtg
     1561 gcctgagcca gcaggtcgat ggcgccgtac aacttgtcct gatcctccgg cttgacggcc
     1621 agcacggtca ggcccagctc ctccatctgg gggatggaat ccttcagctt ggcgggcacg
     1681 atgacaaggt cgggggtcag cgcggcgcag ccctcaaggt caaactcctt ggcggtgccg
     1741 atgctgggca ggctctgcag ctcgggggcg gagaggctgt agatcgtgcg cttgtcggcc
     1801 ttggcctcga cgccgaccag ctcgtcctgc acgccgaggg caatgagcag gctggtggag
     1861 atgtagtagc cgctggccag cgtcttgggc tcggtctcga tggtgacctc gcggcccagc
     1921 tggtcggtga cggtcagcgg gtaggtgacg gcggtctcgg cggcggcggg tgcagcggta
     1981 gcctcctcgg ggctggcggt gacctcgggt gcagcggtgg cggaggcggc ggcctcgctg
     2041 cttacggcgg cggtgctgcc gcaggcggtc agagccatgg cggcggccag cagcagggca
     2101 gtcattttgg tcattttcat ggatcttctt ccctcataca ataaaaaata tcgttgtatt
     2161 tttaaaacaa caacggtatt ataccagatt ttatggtaga ataaaagtac ctatccacga
     2221 aaagtgtgat tttttcgccc aaaacagaaa aaacggagat tgacccatga tgacgatacg
     2281 cgaatatgcc cgccccgcca cgctggagga ggcatggcag ctgaaccaga aaaagcccaa
     2341 ccgcgtgctg ggcggtatgc tctggctcaa gatggaaaag atcaatgtcg gcacggccat
     2401 cgacctgtcg gccttggggc tggatacgat cgaggagact gacaccgaga tcatcatcgg
     2461 cgctatggca acgctgcgcg atctggaaac gaacgccgcc ctgaatgcct ataccgcggg
     2521 ggctgtgcgg gaggctgtgc gccacattgt cggcgtgcag ttccgcaact gcgccacggt
     2581 gggcggcagc atctatggcc ggttcggctt ttccgatgtg ctgacgctgt ttttagcgct
     2641 ggactgcgag gtcgagctgt acagggcggg ccggatgccg ctggcgcagt ttgccgcgat
     2701 gccctatgac cgcgatattc tgacccacat ctgcctgcgc aagacgccgg ggctgaaggt
     2761 gcagtaccag tcggtgcgcg cgacccagac cgatttcccg atcctgacct gtgcggccgc
     2821 ccgcacggcg caggggcagt accgcttttc catcggcgcg cgcccgatga aggccgtgct
     2881 tgtcacgccg gactgcgacc cggcgctgct gcccgccacc gtgcaggcag ccgtcaagac
     2941 cggcagcaac ctgcgcggca gtgccgagta ccgcacccac ctgaccggtg tgctggtccg
     3001 gcgtgccgtg gccgcgctgg aaaattgacg gaggtatacc atgcagatca catttacctt
     3061 aaatcataaa aaagtcaccg ccgaaatcgc ggctgacacc gtgctgctgg accttgtgcg
     3121 cagcctcggc tgcaagagcg tcaagcgcgg ctgcgagacc gccaactgcg gcctttgcac
     3181 ggtctttctc gatgaaaagc cggtgctgtc ctgctcggtg ctggcggcgc gggtcaacgg
     3241 ccgcagcgtc accacgctgg aggggctgca ggaggaggct gccgagttcg gcgcctttat
     3301 cgccgatcag ggtgcggagc agtgcggctt ctgcaacccg ggctttatta tgaacgcgct
     3361 ggcgcttttc cgggaaaacc ccgaccccgg cgagga
//
LOCUS       DBMDSQ010000222         3380 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_6366_length_3380_cov_1.705703, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000222 DBMDSQ010000000
VERSION     DBMDSQ010000222.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 3380)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3380)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3380
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6366_length_3380_cov_1.705703"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1423)
                     /locus_tag="ACZPAX_07950"
     CDS             complement(<1..1423)
                     /locus_tag="ACZPAX_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005924018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HIR1113869.1"
                     /translation="MSEKVKIKIARNVVHLPAIALRGLVVFPNNVVHFEVGRTKSIAA
                     IEAAMHANSSVFLIAQREMDIEEPTIRDLYTYGVIAEIKQVLRVSDDLVKVLVEGKTR
                     ARLLELDAGEKYLQATVRPVAVRGIAADKRNQVEALVRSLKDCFEEYLSYSPQISKDV
                     VYNIISSDSPLFLSEYMPANLLFKYEDKQTILNESTLLGRLEKLLTLLRQECQIMDIE
                     RDLDDKVNARMDKGQREYYLREQMNVISEELGDAEDTRAEADTYREKVKALNLDEEST
                     EKLLKECDRLARMQGSSAESGVIRSYLDACLALPWHTATEDDLDQAHARKVLDREHYG
                     LQKVKERILELLAVRKLAPDIKGQIICLVGPPGVGKTSIARSIAKSMGRKYTRISLGG
                     VRDESDIRGHRKTYIGAMPGRIMNAMKLAGSRNPLMLLDEVDKLGNDYRGDPSAALLE
                     VLDSEQNHAFRDHFIEVPFDLSDV"
     gene            complement(1416..2399)
                     /locus_tag="ACZPAX_07955"
     CDS             complement(1416..2399)
                     /locus_tag="ACZPAX_07955"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol/lipid kinase family protein"
                     /protein_id="HIR1113870.1"
                     /translation="MQPVFIINPTAGKLDASVALIPQIHAAASRAGVKPIVEITRRPG
                     HACDLAAKYAASGEEVALYACGGDGTLNEVLQGVIGRPNAAVGCVPCGSGNDYVRNFG
                     TQAQFLDIDAQLAAKPFAVDLIQTPQGYGIDIYAAGIDAQVANGIPKWRRVPLCGGTT
                     AYTLSILEAICSTFKHRLRITADDRRLEDTFMMLAVCNGQMYGGGYCAAPHASMADGL
                     LDVVLLKPIPRLKLPGLLGGYKKGEHLAAGDAVTEKFKPYLTFFRTSKIDIEVLDGNP
                     LITTLDGECSPQLTMHAEAVRSAAHILLPSALAETANKTPVLAGYGRVTNV"
     gene            complement(2647..3276)
                     /locus_tag="ACZPAX_07960"
     CDS             complement(2647..3276)
                     /locus_tag="ACZPAX_07960"
                     /inference="COORDINATES: protein motif:HMM:NF013100.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HIR1113871.1"
                     /translation="MKLGQLLGAEAIRDEAEHFGFGTAVQIAPGLAAAAGTLPDAETL
                     ENPGQLATFSFGQGSLTVTPLQITAMMNTVANGGVYRTPGFVQGITGADGMLTDPAAA
                     ESAPRTVCDAATARVLRSMLASVVTDGIGHDAQPRHGTAGGKTGTAQTGQYNADGEEL
                     LNYWFTGFYPADAPQYTITVMQDGILKPETSSAALFERITEILDVWAGY"
BASE COUNT          601 a         1036 c         1023 g          720 t
ORIGIN      
        1 gcacgtcgga gagatcgaac ggcacctcga taaaatggtc gcggaacgcg tggttctgct
       61 ccgagtcgag cacctccaga agcgccgcgg acgggtcgcc gcggtagtcg tttccaagct
      121 tatcgacctc gtcaagcagc atcagcggat tgcggctgcc ggcaagcttc atcgcattca
      181 tgatccgtcc gggcatcgcg ccgatgtacg ttttgcggtg gccgcgaata tcgctctcat
      241 cgcgcacgcc gccaagcgaa atgcgcgtgt acttacgtcc catcgatttt gcaatcgacc
      301 gcgcgattga tgttttgcca acgcccggcg gtcccacaag acagatgatc tgccccttga
      361 tatccggcgc gagcttgcgc acagccaaaa gttcgaggat gcgctccttg accttttgca
      421 ggccgtagtg ctcgcggtcc agcaccttgc gggcgtgggc ctggtcgagg tcatcctcgg
      481 tggcggtatg ccacggcagg gccaggcagg cgtccagata gctgcggatg acgccgctct
      541 cggcgctgct gccctgcatc cgggccaggc gatcacattc tttcagcagc ttttccgtgg
      601 attcctcatc gaggttcagc gccttgactt tctcgcggta tgtatcggcc tcagcgcggg
      661 tgtcctcggc gtcgcccagt tcctcactga tgacgttcat ctgctcgcgc aggtagtatt
      721 cgcgctggcc cttgtccatg cgggcgttga ccttatcgtc aaggtcgcgc tcgatatcca
      781 tgatctggca ttcctgccgc agcagggtca agagcttttc aaggcggccc aacaaggtcg
      841 attcgttcag aatcgtctgc ttgtcctcat acttgaacag caggttggcg ggcatatact
      901 cgctcaaaaa cagcggcgag tcggaagaaa tgatgttata gacaacatcc ttggagattt
      961 gcgggctgta cgagaggtat tcctcgaagc agtctttcag gctgcggaca agggcttcga
     1021 cctgattgcg cttgtcggcg gcaatgccac gcaccgcgac cgggcgcacg gtggcctgca
     1081 aatacttctc ccctgcatcc agttccagca ggcgggcgcg ggtcttgccc tcgaccagga
     1141 ctttgacgag atcgtcggag acgcgcagca cctgcttgat ctcggcaatg acaccgtagg
     1201 tgtacaggtc gcggatggtc ggttcttcga tgtccatttc ccgctgggcg atcaggaaca
     1261 cgctgctgtt ggcgtgcatg gcggcctcga tggcggcgat agacttggtg cggcccacct
     1321 caaagtggac aacgttgttc gggaacacca ccagcccgcg cagcgcgatg gccggcagat
     1381 gtaccacatt gcgggcaatc ttgattttta ctttttcaga cattcgttac tctcccataa
     1441 cctgccagaa caggcgtctt gtttgcggtt tcggccaagg cggacggcag caggatgtgc
     1501 gccgcactgc gcacagcttc cgcgtgcatc gtcagctggg gcgaacactc gccgtccagc
     1561 gtcgtgatga gcgggttgcc gtccagcacc tctatgtcaa ttttgcttgt acggaaaaac
     1621 gttaagtagg gtttgaattt ttctgtcacg gcatcgcctg ccgccaggtg ctcacccttt
     1681 ttgtagccgc ccagcaggcc gggcagcttt aagcgcggga tgggtttcag cagcaccacg
     1741 tccagcaggc cgtccgccat gctggcgtgg ggcgcggcac aatagccgcc gccgtacatc
     1801 tggccgttgc agactgccag catcatgaag gtatcttcca gccggcggtc gtccgccgtg
     1861 atgcgcagac ggtgcttgaa ggtgctgcag atggcttcaa gaatcgacag cgtgtaagcg
     1921 gtcgtgccgc cgcacagcgg tacgcgccgc cacttgggga taccgtttgc cacctgcgcg
     1981 tcaatgccag cagcgtagat atcaatgccg tacccctggg gcgtctgaat cagatccacc
     2041 gcgaacggct ttgccgccag ctgggcatcg atatccaaaa actgcgcctg ggttccgaag
     2101 ttgcgcacgt agtcgttgcc gctgccgcag ggcacacagc ccacagcggc attggggcgg
     2161 cctatgacgc cctgtaaaac ttcgttcagc gtgccgtctc cgccgcaggc gtatagggct
     2221 acttcttccc cgctggcggc atatttggcg gccaggtcgc aggcatggcc cgggcggcgg
     2281 gtgatctcca cgatgggttt taccccggcg cggctggcgg cggcgtggat ctgcgggatc
     2341 agcgccacgc tggcatccag cttgcccgcc gttggattta taatgaatac aggctgcatc
     2401 ggtttgtccc tttcagcacc aggccattgt ggtttacaga taataattat tgtagctttt
     2461 ttaccgcaaa agtgcaatac cctgggggaa attggatgca ataaatggct ttccccgagg
     2521 gggaagctgt ccgcgaagcg gactgatgag gggcggtctt gccatgtttg cccataaagg
     2581 ggttgccatg ggacacgcac ccctcatccg gccctgcggg gccgccttcc cccgagggga
     2641 gaaggcttag taccccgccc acacatccag aatttccgtt attcgttcaa acagggcggc
     2701 gctggaggtt tcgggcttca agataccgtc ctgcatgacc gtgatggtat attggggcgc
     2761 atcagccggg taaaagccgg tgaaccaata gttcagcagc tcctcaccgt cggcgttgta
     2821 ctggccggtc tgggccgtgc cagtcttgcc gcccgctgta ccatggcgtg gctgggcgtc
     2881 atggccgatg ccatccgtca ccaccgaggc cagcatactg cgcagcaccc gggccgtggc
     2941 ggcgtcacag acggtacgcg gggcgctctc cgctgccgcc gggtcggtca acataccatc
     3001 ggccccggtg atgccctgca caaagccagg tgtcctatac actccgccgt tggcaacagt
     3061 gttcatcatg gcggtgattt gcagcggcgt cacggtcagc gacccctgcc caaagctgaa
     3121 cgtcgccaac tggccggggt tttccagcgt ctccgcgtcc ggcagcgtac cggcagctgc
     3181 ggccaggccc ggggcaattt gtaccgctgt gccaaagccg aagtgttctg cctcgtcccg
     3241 gatggcctct gcccccaaca gctggcccag tttgataaac taggtgttgc agctttcttc
     3301 cagcgcaccg cgcaggttga cttcgccgtg ggcgcggccc tgagcgcagc ggtaggtctg
     3361 ccccgccacc acgatttcac
//
LOCUS       DBMDSQ010000223         3353 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_6414_length_3353_cov_1.480012, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000223 DBMDSQ010000000
VERSION     DBMDSQ010000223.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 3353)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3353)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3353
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6414_length_3353_cov_1.480012"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..408)
                     /locus_tag="ACZPAX_07965"
     CDS             complement(<1..408)
                     /locus_tag="ACZPAX_07965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113872.1"
                     /translation="MEEEKNTAAAVDWEEAPNPKHRKRSRKKEPAANAPRPAETAVSA
                     ADSAPDAEPDTASDTPEQPEPAEPAETPAAPETVGRDVPIPPQPADAPAEEAAPTDTP
                     EDTPEASAKSADPAEAESAPAEPDDAPTEQESAS"
     gene            complement(529..1761)
                     /locus_tag="ACZPAX_07970"
     CDS             complement(529..1761)
                     /locus_tag="ACZPAX_07970"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC family protein"
                     /protein_id="HIR1113873.1"
                     /translation="MRKLLLWLVMVAVMVAAILGGTAAFLYNRTGEDALPQEPVQFGD
                     ITLESNGWDWAVPVLGDKVTKTYESPTNLTVQKLGTFTDTIPQLMLPAWVTSATVQIT
                     APDGTAWSGDKSSCNTYTYAQNGDYQIIVTAYHNDADAPGDPAGWYAYRAGFTMAMNP
                     KVTLSTERAAQGSIVAIQLSGILDGEPSVESDLGTVWFRKTSSGYMGYLPVTYNAEGG
                     DHTLHLTCGTLEKDITLTVTRTMYDTQAVPAEEDVGGGEEFRNAIWPLYTTGSSAKLW
                     NGRFEAPSAGAVTMEYGTTQMVDSQRSGQATGLTYDAPEGETVTAPQAGNVVFAGTLT
                     LTGGTVVIDHGCGVKSYLYGLETVDVERGQSVSTGDPLGTAGTTHNLIYELRIGSKST
                     DPQIALNGNSGLQFRESE"
     gene            complement(1837..2052)
                     /locus_tag="ACZPAX_07975"
     CDS             complement(1837..2052)
                     /locus_tag="ACZPAX_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015564446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HIR1113874.1"
                     /translation="MMKKSTFGAVLAFLFAAAGALTAAALYLYHREKELDEYERLLFS
                     DDYDDFDDEDDEDTTTYEKIPDEPTEK"
     gene            2309..>3353
                     /locus_tag="ACZPAX_07980"
     CDS             2309..>3353
                     /locus_tag="ACZPAX_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1113875.1"
                     /translation="MQNTFARESVLKNVLRFSLPYLLSYFLQTLYGMADLLIIGQFDG
                     VAGTTAVSIGSQVMHMVTVMLVGLAMGITVGIGHAVGAGENRRAGSTIGNGAVLFLVL
                     SAALAALLAGLVGPITAIMSTPAEAVAGTRAYLTICFIGIPCITAYNIIAAIYRGLGD
                     SKSPMYFIAVACAANIGLDYLFMGALHMGPAGAALGTTLAQGISVVVSLGVILRRKSG
                     IRLQKSDLRPQKSVIGRILRIGVPVALQDGLIQISFLLITVIANRRGLTDAAAVGIVE
                     KIISFIFLVPSSMLSTVSALGAQNVGAGKHDRAAKTLYYAIGISLTFGAIVGVLVQFI
                     APNAVALFTSDAAV"
BASE COUNT          552 a          987 c         1072 g          742 t
ORIGIN      
        1 gctggcagat tcttgttcgg tgggagcatc gtccggttca gcaggggcgg attcggcttc
       61 ggctggatcg gcagacttcg cagatgcctc gggcgtatct tcgggggtat ccgtaggtgc
      121 agcttcttcc gccggggcgt cggcaggctg cggcggaatg gggacatccc gacctacggt
      181 ctccggggca gcgggggtct ccgccggttc ggcgggttcg ggctgctcgg gggtatcgga
      241 tgccgtgtca ggttcggcat ccggggccga gtcagccgca gaaacagccg tttcggcggg
      301 cctgggggca ttggcggccg gttccttttt gcggctgcgt ttgcggtgct tcgggttggg
      361 tgcttcctcc caatccacgg cagccgcggt gttcttctct tcttccatgg tttctcccac
      421 tcttctaata actcgattcc ctaggatgta gggggcggta tcctcgacgc cccgcaactt
      481 ttgggcgggc ggtcgtttgc ccttcgtgtg gggcccgccg tataatattt attccgactc
      541 tctgaattgc agaccactgt tgccgttcag ggcgatctgc gggtcggtgg atttgctgcc
      601 aatgcgcagc tcataaatca aattgtgggt cgtcccggca gtgcccagcg ggtcgccggt
      661 ggaaaccgac tgcccgcgct ccacatccac ggtttccagg ccgtagaggt agctcttgac
      721 gccgcagccg tgatcgatga ccaccgtgcc gccggtcagg gtcagcgtgc ccgcaaagac
      781 cacgttgccc gcctggggcg cggtgacggt ctcgccttct ggggcgtcgt aggtcaggcc
      841 ggtggcctgg ccgctgcgct ggctgtccac catctgggtc gtgccgtatt ccatcgtgac
      901 agcgcccgcg ctgggggcct caaagcgacc gttccacagc ttggcagaac tgccggtggt
      961 gtacagcggc cagatggcat tgcggaattc ctcgccgccg cccacgtcct cctcggcagg
     1021 gacagcctgg gtatcgtaca tcgtgcgggt cacggtgagg gtgatatctt tttccagcgt
     1081 gccgcaggtc aagtgcagcg tgtggtcacc gccctcggcg ttgtaggtca cgggcaggta
     1141 gcccatgtac ccgctggacg tcttgcggaa ccagaccgtg cccaggtcgc tttcgacact
     1201 gggctcgccg tccagaatgc cggacagctg gatagcaacg atgctgccct gggccgcacg
     1261 ctcggtgctc agggtcactt tggggttcat ggccatcgtg aacccggcgc ggtaggcgta
     1321 ccagcccgcc gggtcgccgg gggcgtcggc gtcgttgtgg taagccgtta cgatgatctg
     1381 ataatcaccg ttctgggcat aggtataggt gttgcagctg gatttatcgc cgctccaggc
     1441 tgtgccgtcg ggcgcggtaa tctgcaccgt ggccgatgtg acccatgcgg gcagcatcag
     1501 ctgggggatg gtatcggtga aagtgcccag cttctgcacg gtcaaatttg tggggctttc
     1561 gtatgttttt gtgactttgt cacccagcac cgggacagcc cagtcccagc cgttggattc
     1621 cagcgtgata tcgccgaatt gcacaggctc ctgcgggagg gcgtcctcgc cggtgcggtt
     1681 gtacaggaat gccgctgttc cgcccaggat cgcggcaacc atcacggcga ccatcaccag
     1741 ccaaagcagc agtttacgca taattcatat tctccattac caaaagaccg gacgccgcca
     1801 gcgcccggcc ggaaggtgtt ttgcacggga aggaaattac ttctccgtcg gttcgtcggg
     1861 gatcttctcg taggtggtgg tgtcctcgtc gtcctcgtcg tcaaaatcat cgtaatcgtc
     1921 gctgaacagc aggcgctcgt actcgtccag ctccttctcg cggtggtaca ggtacagcgc
     1981 ggcggcggtc agcgcaccgg cagcggcaaa caggaaggcc agaacggcac cgaaggtaga
     2041 tttcttcatc ataatgggtt ctcctttaca taaaaactgc acagatgcgc gagttttgga
     2101 aacgcataca gaaatggatg atttgctgat tctattatac ccgacgcggc gggcaaatac
     2161 aacactgcaa tttgtaaaat attcctggat ttttgcgccg ctttttgccg gaaatgtgat
     2221 gccgcccggc tctgcctcgc cttgacaagc ggccccgaat gcggtttact atattagtac
     2281 tttaaatcgg aaaagaggat aatcccctat gcaaaacacc tttgccaggg agagtgtact
     2341 aaaaaatgta ctgcggttct cgctgccgta cctgctctcc tactttttgc agacgcttta
     2401 cggcatggcg gacctgctca tcatcggcca atttgacggt gtggcgggca cgacggcggt
     2461 gtccatcggc agccaggtca tgcacatggt cacggtcatg ctggtggggc tggctatggg
     2521 tatcacggtg ggcatcggcc acgccgtcgg tgcgggcgaa aaccgccgcg cgggcagcac
     2581 catcggcaac ggcgcagtgc tgttcctggt gctctcggcg gcgctggcgg cgctgctggc
     2641 ggggctggtg gggcccatca cggccatcat gtccaccccg gccgaggccg ttgcgggcac
     2701 ccgggcgtac ctgaccatct gttttatcgg catcccctgc atcacggcgt acaacatcat
     2761 tgcggccatc taccgcgggc tgggagactc taaaagcccg atgtacttta tcgcggtggc
     2821 ctgcgcggcc aacatcgggc tggactactt gtttatgggg gcactgcaca tgggccccgc
     2881 cggtgcggcg ctgggtacta cgctggccca gggtatcagc gttgtcgtct cgctgggggt
     2941 cattctgcgg cgcaaaagcg gcatccggct gcaaaaaagt gacctgcggc cgcagaaaag
     3001 tgtcattggc cgcatcctgc gcatcggcgt gccggtggcc ttgcaggacg gacttatcca
     3061 aatctcgttt ttacttatca cggtcattgc caaccggcgc ggcctgacgg acgccgccgc
     3121 tgtgggcatc gtggagaaga tcatcagctt catcttcctg gtgccgtcct cgatgctgtc
     3181 cacggtgtcg gcgctggggg cccagaacgt gggcgcaggc aagcacgacc gcgccgccaa
     3241 gacgctgtac tatgctatcg gcatttcgct gacctttggg gccatcgtgg gtgtgctggt
     3301 gcagtttatc gcccccaatg ccgtggctct gtttaccagc gacgcggctg tgg
//
LOCUS       DBMDSQ010000224         3350 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_6417_length_3350_cov_1.707295, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000224 DBMDSQ010000000
VERSION     DBMDSQ010000224.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 3350)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3350)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3350
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6417_length_3350_cov_1.707295"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..701
                     /locus_tag="ACZPAX_07985"
     CDS             <1..701
                     /locus_tag="ACZPAX_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HIR1113876.1"
                     /translation="MNDTLDYFKKTPDERRENLGKLTFSMMYAWNEHYILPFSHDENV
                     HGKATIVQKMYGDYEGKFPQARALYLYMAIHPGKMLDFMGNEFAQLREFDESREQDWM
                     VLKYPSHDDFHRYRKALNEAYCANEAFWSREYDPVAFQWLDCRHPELDACAIWREGHD
                     GAVLAAFNFGDTELKEYELTLPRAGRLTPILDTDWQCFGGQTEKPKRLVGKACEGTLK
                     LTLAPFSGRLYKLV"
     gene            complement(837..2354)
                     /locus_tag="ACZPAX_07990"
     CDS             complement(837..2354)
                     /locus_tag="ACZPAX_07990"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007045727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HIR1113877.1"
                     /translation="MDLRRLIQNTDCQLVRGDALTEVTALCCDSRCAGPGALFVCLPG
                     RHTDGRQFAAQAVAAGAVAVLCAGPPPDVGAAVVETENPRRMMAVLAARLCGEPARAM
                     TMIGVTGTKGKTTAAHLLAAILQADGRRVGLVGTNGVCWPGHRHDLNHTTPESCDLQA
                     LLRRMADDGCDTCVMEVSSLGMKFDRAAGIEFDVGVFTNLSPDHIGPGEHADFAEYLH
                     WKAALFQQCRAGVFNIDDPWVGKIVDGVPCRVVTYGTVPGADVRADSNFTLTREGGRL
                     GAAFTVDGVRYTVGMPGAFSVQNALAALAAARVLGAGEDAIRRGLAAAVVQGRVEPVA
                     VDAPFTVLIDYAHNEAAAESLLRTLRAYTPARLVAVFGCGGERSRLRRFGMGSVCARL
                     ADFCVITEDNSRSEPMEHILADIRAGLKLGNPATPFAEIPDRRQAIYYALAHAQPGDI
                     VAILGKGHETTIERNGVKEPFVEREVVEEFFSLPSPKGEGGPAGPDEGRLFEGRP"
     gene            complement(2361..>3350)
                     /locus_tag="ACZPAX_07995"
     CDS             complement(2361..>3350)
                     /locus_tag="ACZPAX_07995"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:NF012936.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0070981 - L-asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthetase B family protein"
                     /protein_id="HIR1113878.1"
                     /translation="YTISYAEPKYDESFPARALARNLGLRNRVRCISPGEFWDAVPAV
                     QYHMDEPMADAAAVALYFLNREAARDVKVVLSGEGADELFGGYNIYRDPFTARWYNRL
                     PPWLRGGLGAAASLLPPGPGVNFLVRRGLSLEERYFGPTALLTEREKRRLLPGYEGDG
                     DPVCLTESIWDMTEDQDPVTRMQQVDLQLWLAGDILLKADKMSMAHSLELRVPYLDKE
                     VFALAAALPAAAKANARMTKIALRQAAARTLPPAAAARKKLGFPVPVRDWLRQEPYTS
                     RVRAVFSRPAAGEFFNVRLLHTMLNHHLHGGDCWRQIWCVYSFLIWYEQFFGA"
BASE COUNT          610 a         1147 c         1054 g          539 t
ORIGIN      
        1 ggatgaacga taccctagac tactttaaaa agaccccgga cgagcggcgg gagaacttgg
       61 gcaagctgac cttctcgatg atgtacgcct ggaacgagca ttacattttg cccttcagcc
      121 acgacgagaa cgtccacggc aaggcgacca tcgtgcaaaa aatgtacggc gactacgagg
      181 gcaagttccc ccaggcgcgg gcgctgtacc tgtacatggc tatccacccg ggcaagatgt
      241 tggactttat gggcaacgaa ttcgcgcagc tgcgcgagtt tgacgagagc cgcgagcagg
      301 actggatggt gctgaagtac ccgagccacg acgatttcca ccgctaccgc aaggcgctga
      361 acgaggcgta ctgcgccaac gaggcgttct ggagccgtga gtacgacccg gtggcgttcc
      421 agtggctgga ctgccggcac cccgagctgg acgcctgtgc catctggcgt gagggccatg
      481 acggcgcggt gctggcggcg ttcaacttcg gcgatacgga gctgaaagag tacgagctga
      541 cactgccccg cgcgggcagg ctgacgccga ttctggacac ggattggcag tgcttcggcg
      601 gccagaccga gaaaccgaag cggcttgtgg gcaaggcgtg cgaaggcacg ctgaagctga
      661 cgctggcacc gttcagcggg cggctgtata agttggtata actgcatata aaaaggccgc
      721 gcctttcccg atacaggatg ggcgcggcct tttgctgtgc ggtaggggcc gggcgtgcca
      781 ggcccggccg caaagggtga ggcgagcggt tgtggggacg gtttctcgct gtcacctcac
      841 ggtctgccct caaaaagccg cccctcatcc ggccctgcgg ggccaccttc ccccttgggg
      901 gaaggcaggg agaaaaactc ctccactacc tcccgctcca caaacggctc cttgacccca
      961 ttgcgttcta tcgtcgtctc gtggcctttg cctaaaatcg ccacgatatc cccgggctgg
     1021 gcgtgggcca gcgcgtagta aatggcctgc cgccggtccg gtatctcggc aaagggggtg
     1081 gccgggttgc caagtttcag ccccgcccgg atatccgcca aaatatgctc catcggctca
     1141 ctgcggctgt tgtcctcggt aatgacgcag aaatccgcca gccgggcgca gacggacccc
     1201 ataccgaacc gccgcagccg gctccgctcg ccaccgcagc cgaacaccgc caccagccgc
     1261 gccggggtgt aggcccgcag cgtgcgcaac aggctctcgg cggcggcctc gttgtgggcg
     1321 tagtcgatca gcaccgtgaa cggtgcgtcc acggccacag gctccacccg gccctgcacc
     1381 acggcggcgg ccagcccccg gcgtatcgcg tcctcgcccg cgcccagcac ccgcgctgcg
     1441 gccagggcag ccagggcgtt ctgcacgctg aacgccccgg gcatccccac ggtgtaccgc
     1501 acaccgtcca ccgtgaaggc cgcgcccagc cgaccgccct cccgtgtcag agtaaaattg
     1561 ctatcggccc gcacatcggc cccggggacc gtgccgtagg ttacgacgcg gcaggggacg
     1621 ccgtccacga ttttccccac ccaggggtcg tcgatgttga aaactcccgc gcggcactgc
     1681 tgaaacagcg cggctttcca gtgcagatac tcggcaaaat ccgcgtgctc gccggggccg
     1741 atgtggtcgg gcgacaggtt cgtgaacacg cccacgtcaa actctatccc cgccgcgcgg
     1801 tcaaacttca tccccaggct tgaaacttcc atcacgcagg tgtcgcagcc gtcgtcggcc
     1861 atgcggcgca aaagcgcctg caagtcacag ctttccggcg ttgtgtggtt caaatcgtgg
     1921 cgatggccgg gccagcagac gccgttggtg cccaccagcc cgacccgccg cccatcggcc
     1981 tgtaaaatgg ccgccagcag gtgggccgcc gtcgtcttgc ccttcgtgcc ggtcacgccg
     2041 atcatcgtca tggcgcgggc cggttcaccg cagagccgcg ccgccaaaac cgccatcatg
     2101 cggcgtgggt tttccgtctc taccacggcc gcgcccacgt ccgggggcgg cccggcgcac
     2161 agcaccgcca cagccccggc ggccacggcc tgggccgcaa actgccgccc gtcggtgtgg
     2221 cggcccggca ggcagacaaa cagcgccccc ggcccggcac agcgggaatc acagcagagc
     2281 gccgtcacct cggtcagggc gtcgccccgc acaagctggc agtcggtgtt ctggataagc
     2341 cgccgcagat ccatttggca tcacgctcca aaaaactgtt cgtaccaaat caaaaaactg
     2401 tacacgcacc agatctgccg ccagcagtcg ccgccgtgca ggtggtggtt caacatagta
     2461 tgcagcagcc gcacattaaa gaactctccg gctgccgggc ggctgaatac ggcccgcacc
     2521 cggctggtgt acggctcctg ccgcagccag tcccgcaccg gcactgggaa acccagcttt
     2581 ttccgggcgg cagccgccgg gggcagtgtg cgggccgcag cctggcgcag ggcaattttt
     2641 gtcatgcggg cgttggcctt ggcggcggcg ggcagcgcgg cggccagggc aaagacttct
     2701 ttatcaaggt agggcacccg cagctccagg ctgtgggcca tgctcatttt gtcggctttc
     2761 agcaaaatat ccccggccaa ccagagctgc agatctacct gctgcatccg ggtcacaggg
     2821 tcctgatcct cggtcatgtc ccagatggat tcggtcaggc agaccgggtc gccgtcgccc
     2881 tcgtacccgg gcagcaggcg gcgcttttcc cgctcggtca aaagcgccgt ggggccaaag
     2941 tagcgttctt ctaaacttaa accccgccgc acaagaaaat ttacccccgg cccgggcggc
     3001 agcaggctgg cggcggcccc cagcccgccc cgcagccagg gcggcaggcg gttataccac
     3061 cgcgcagtga agggatcgcg gtagatgtta tacccgccga acagctcgtc cgcgccctcg
     3121 cccgagagga cgaccttcac atccctggcg gcctcacggt tcaaaaagta gagcgccacg
     3181 gcggccgcgt cggccatcgg ctcgtccatg tggtactgca cagcgggtac ggcgtcccag
     3241 aactcgccgg ggctgatgca ccgcacccgg ttccgcagcc ccaaattccg cgccagcgcc
     3301 cgggcgggga aggactcgtc gtacttcggc tctgcgtagg aaatcgtgta
//
LOCUS       DBMDSQ010000225         3332 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_6446_length_3332_cov_1.643145, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000225 DBMDSQ010000000
VERSION     DBMDSQ010000225.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 3332)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3332)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3332
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6446_length_3332_cov_1.643145"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(171..254)
                     /locus_tag="ACZPAX_08000"
     tRNA            complement(171..254)
                     /locus_tag="ACZPAX_08000"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(218..220),aa:Leu,seq:taa)
     gene            456..1838
                     /gene="miaB"
                     /locus_tag="ACZPAX_08005"
     CDS             456..1838
                     /gene="miaB"
                     /locus_tag="ACZPAX_08005"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020073503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HIR1113879.1"
                     /translation="MEKLTYTHNDAAAKVLAAYYETPPLAYVRSYGCQQNVNDGEKIK
                     GVLQDVGYGLCDNVEHADLILFNTCAVREHAEQRVFGNVGALKKLKEQNPRLMIGICG
                     CMAQQEHIVEKLRQSYPYVDLVFGVDGIDRLPAMLAERIRKGKRYLEKPEERNAVVEE
                     LPIRRDSGFRAWLPIMYGCDNFCTYCIVPYVRGRERSREPAAILAEFKQLVAQGYKEI
                     TLLGQNVNSYGKGLENPIDFADLLNLLCEVPGDYQIRFMTSHPKDASRKLIDTIAAQE
                     HLCKHIHLPVQSGSDRLLKEMNRHYNVAQYMDLINYAKEKVPGVTFSSDIIVGFPGET
                     EEDFAATLDLIRKVGYMQLFTFIYSKRMGTPAAKLPDPTTHAEKAARMDRLLKTQEEF
                     AFAATAAMTGQTVRVLVEAAGRSEGTVNGRLDNNLVVEFKAPETLIGQWADVHVTGSR
                     AALLVGELAE"
     gene            1875..2282
                     /locus_tag="ACZPAX_08010"
     CDS             1875..2282
                     /locus_tag="ACZPAX_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015564067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA];
                     GO_process: GO:0043934 - sporulation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF/YmcA family competence regulator"
                     /protein_id="HIR1113880.1"
                     /translation="MDCIDLFKKAAAALQTDVRYLELDAARRENDADEELQNMIGEFN
                     LARLDLNNESAKPETDAARVAELNQRVNDLYSQIMASEGMVRYNDAKAECEALVSHID
                     AIINTAMNGGDPMTVQAPTGGCTGSCSTCGGCH"
     gene            2433..>3332
                     /locus_tag="ACZPAX_08015"
     CDS             2433..>3332
                     /locus_tag="ACZPAX_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015538562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MutS N-terminal domain-containing protein"
                     /protein_id="HIR1113881.1"
                     /translation="MAEQAVSPMMQQYFEIKKQHPNEILFYRVGDFYEMFYDDALTAS
                     RELELTLTGKNCGREERAPMCGVPFHSYETYMARLVAKGYKVAICEQIEDPALAKGLV
                     KRDIIRVVTPGTVIESSMLREDKNNYLASIFCKRTRGRWRAGVCFADISTGEAYATEL
                     NAEKIGGAIITELCRYMPSEILICPPMLDFKDVTTYIKQHTNALVELREDACFKDTVM
                     ESTMADQFGANWRETLGYEKDALVPYAVAALLNYLHETQKHGVERIKTVQNYAYAQYM
                     RLSPVTRANLELTETMRGREKRGT"
BASE COUNT          795 a          989 c          919 g          629 t
ORIGIN      
        1 agaaattata ccgcaaaatc gagggattgt caacagaatc gagggatttt tggccgaaaa
       61 aatactaggg attgccggaa taaaattgac tagggattac ttttgacaca cagaaatcaa
      121 aacaaaaaag gccctatatt cagcgaataa acgttgaata tagggctgta tgccgctgac
      181 gggaatcgaa cccgtacgat gtttccgtcg agggatttta agtcccttgc gtctgccagt
      241 tccgccacag cggcataaca tatgtattgt agcgcatgat ggcggaaatg tcaatcttcc
      301 cggcgcgttt ttttcatccc aaaacccaat ttgcacaaaa aaatcaacat cagccgcttg
      361 tgcttttgcc cgagatttgc tatactaata tggtatgttt ggataggtgc gcccatttgg
      421 caagacccgg gcgttttata ataaggagaa ttcacttgga aaaactgacc tatacccaca
      481 atgacgctgc ggccaaggtg ctggcggcct actacgagac cccaccgctg gcgtatgtgc
      541 gcagctacgg ctgccagcag aacgtcaacg acggagagaa aatcaagggc gttttgcagg
      601 atgtcggcta cggcctgtgt gacaatgtcg agcacgccga cctcatcctg ttcaacacct
      661 gtgccgtgcg tgagcacgcc gagcagcggg tgttcggcaa tgtcggtgcg ctgaaaaagc
      721 tcaaagagca gaacccccgc ctgatgatcg gcatctgcgg ctgcatggcc caacaggagc
      781 atatcgttga aaagctgcgc cagagctacc cctatgtgga tctcgtgttc ggtgttgacg
      841 gcatcgaccg cttacccgcc atgctggcgg agcgcatccg caaaggcaag cgctacctgg
      901 aaaagcccga ggaacgcaac gccgtggtcg aggaacttcc catccggcgg gactccggct
      961 tccgtgcctg gctgcccatc atgtacggtt gcgataactt ttgcacctac tgcatcgtgc
     1021 cttatgtccg cgggcgggag cgcagccgtg aacctgccgc cattttggca gagttcaagc
     1081 agctggtggc ccaaggatac aaggaaatca ccctcttggg ccagaacgtc aacagctatg
     1141 gcaaggggct ggaaaacccc atcgactttg ccgacctgtt gaatcttttg tgcgaggtgc
     1201 ccggcgacta tcagattcgc ttcatgacga gccatccgaa ggatgccagc cgcaagctca
     1261 ttgatacgat tgctgcgcag gagcatctgt gcaaacacat ccatctgccg gtgcagtccg
     1321 ggtctgaccg cctcttgaag gagatgaacc gccactacaa cgtcgcgcag tacatggacc
     1381 tcatcaatta tgcgaaagaa aaggtccccg gcgtgacctt ctccagcgac attattgtcg
     1441 gcttccccgg cgagacggaa gaagattttg ccgcgacgct cgaccttatc cgcaaggtgg
     1501 ggtatatgca gctgttcacc tttatctatt ccaagcgcat gggtacgccc gccgccaaat
     1561 tgcctgaccc cacgactcac gccgaaaagg ccgcccgcat ggaccgcctg ctgaaaacgc
     1621 aggaagagtt cgcctttgcg gccaccgccg ccatgaccgg ccagaccgtc cgcgtgctgg
     1681 tggaggccgc cggccgcagc gagggcaccg tcaatggccg cctggacaac aacctggtgg
     1741 tcgagttcaa agcccccgaa accctcatcg gccagtgggc cgacgtccac gtcaccggct
     1801 cccgcgccgc gctgctggtg ggtgaacttg ccgaataaac ccaatctaca ttatatatac
     1861 aaggagattt taccatggat tgcattgatc tttttaagaa agccgcagct gctttgcaga
     1921 ctgacgtccg ctacctggaa ctggacgccg cccgccgcga gaatgacgcg gacgaggagc
     1981 tgcagaacat gatcggcgag ttcaacctcg cccgcctgga cctgaacaac gagagtgcca
     2041 agcccgaaac cgacgctgcc cgtgtggccg agctgaacca gcgtgtcaac gacctgtaca
     2101 gccagatcat ggccagtgag ggcatggtgc gctacaacga cgccaaggcc gagtgcgagg
     2161 ccctggtcag ccacatcgac gccatcatta acaccgctat gaacggcggc gatcccatga
     2221 ccgtgcaggc ccccaccggc ggctgcaccg gcagctgctc cacctgcggc gggtgccact
     2281 aaagcttgag gcgcggtagg gaactgtagg ggcgagcatt gctcgcccgt cacttcccgc
     2341 tgctgcccat agcccgggca aacggcccgc ggacgggcaa tgcccgcccc tacaaagcaa
     2401 cgtatcaacc agacaaaaca ggagtgtgaa acatggccga acaagcagta tcgcccatga
     2461 tgcagcagta ttttgaaatt aaaaaacagc atcccaacga gatattgttc taccgcgtcg
     2521 gtgattttta cgagatgttc tacgacgacg cgttgacggc ctcccgcgaa ctggaactga
     2581 cgctgaccgg caaaaactgc ggcagggaag aacgcgcccc catgtgcggc gtgccgttcc
     2641 actcctacga aacctacatg gcacgcctgg tggccaaagg ctacaaagtc gccatctgtg
     2701 agcaaatcga ggacccggcc ctggccaagg gccttgtcaa gcgcgatatc atccgcgttg
     2761 tcacccccgg cacagtcatc gagagcagta tgctccgcga ggacaaaaac aactatctag
     2821 cgagcatttt ctgcaagcgc acccggggcc gctggcgtgc gggcgtctgt tttgccgata
     2881 tctccaccgg cgaagcctac gctaccgagc tgaacgctga aaaaatcggc ggggccatca
     2941 tcaccgagct gtgccgttac atgcccagcg aaattctgat ttgcccgccg atgctcgact
     3001 tcaaagacgt gacgacctac atcaagcagc acacgaacgc ccttgttgaa ctgcgggaag
     3061 atgcctgctt caaggacacc gtcatggaat ccacgatggc ggatcagttc ggcgcgaact
     3121 ggcgggagac gctgggctat gagaaagacg ccctcgtgcc ttacgccgtg gctgccctgc
     3181 tcaactatct gcacgagacg cagaagcacg gcgtcgagcg cattaagacc gtgcagaact
     3241 acgcctacgc acagtatatg cgcctgtcgc ctgtgacccg cgccaatttg gaactgaccg
     3301 agacgatgcg cggccgcgaa aagcgcggca cg
//
LOCUS       DBMDSQ010000226         3331 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_6449_length_3331_cov_1.842415, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000226 DBMDSQ010000000
VERSION     DBMDSQ010000226.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 3331)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3331)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3331
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6449_length_3331_cov_1.842415"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..609)
                     /locus_tag="ACZPAX_08020"
     CDS             complement(<1..609)
                     /locus_tag="ACZPAX_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005931939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T family protein"
                     /protein_id="HIR1113882.1"
                     /translation="MAANTVLNYLPRKSVVHELTGTTKLAFFLLFTFAGMITYNTWVL
                     LGLMVVSFAAFKLSHIKLREVRFMLVFMLVFLALNDLFIFLFDPNQGTTLYGTRTVLC
                     HLFWRYDLTAEQLFYMFNISLKYFVALPVAILFISATNPSEFAASLNSIGISYKVGYS
                     VAIALRYIPDIQRDYHSISQAQQARGVELGRSEPFFKRLKNSG"
     gene            complement(599..2323)
                     /locus_tag="ACZPAX_08025"
     CDS             complement(599..2323)
                     /locus_tag="ACZPAX_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005931941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1113883.1"
                     /translation="MAETKTPIISFKNFSFQYRAQKQPTLHDINLDIYPGERVLIAGP
                     SGSGKSTLAACINGLNPFSNPGECTGSLTVDGVDAPNSSIFELADHVGTVLQDPDGQF
                     IGLTVAEDIAFALENSCTPQKEMHETTRRAAELVGIENHLDYAPHELSGGQKQRVSLA
                     GVMVDEVKILLFDEPLANLDPATGKQAIELIDTIQQKTDTTVLIIEHRLEDVLWRSVD
                     RIVLVNDGRILADLRPDELLSGALLAENGIREPLYVTAIRYAGVAITPEKHPAHIDSV
                     VLDDADTAKLQAWFKAEPLPAPKPAPQPLLEVKGLSFGYNKGQRTLTNVSFTIGRGEM
                     VSIVGRNGAGKSTLSKLICGFETPDSGEIFFDGKNLKEENIRRRARHIGYVMQNPNQM
                     ISKTMIFDEVALGLQGSGLSEADTRAKVEDTLKICGLYPFRNWPISALSFGQKKRVTI
                     ASVLVQDPELIILDEPTAGQDFRHYTDIMEFLRGLNAKGVTVVMITHDMHLMLEYTPR
                     ALAFCDGRLIADCSAAAILCDPKLIEQAALKETSLYTLANRCGIAPAEDFVERFIAAD
                     REVRAHGC"
     gene            complement(2407..2955)
                     /locus_tag="ACZPAX_08030"
     CDS             complement(2407..2955)
                     /locus_tag="ACZPAX_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005931943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type riboflavin transporter
                     substrate-binding protein"
                     /protein_id="HIR1113884.1"
                     /translation="MKNNTIKTFVAVGIGAALFFVLGRFVAIPSPVPNTNISLQYAVL
                     GLLAAMYGPVAGGLIGFIGHALIDLSWGGIPWWSWVIASAFTGVVVGLFAKKLNIQDG
                     DFNKAKAAIFAVANVVAHAIAWIVVAPVLDILIYSEPVKKVFAQGAFAAVSNIITAVV
                     VGGLLILAYTKTIAKKGSLDKE"
     gene            complement(3123..3198)
                     /locus_tag="ACZPAX_08035"
     tRNA            complement(3123..3198)
                     /locus_tag="ACZPAX_08035"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3163..3165),aa:Gly,seq:gcc)
     gene            complement(3205..3280)
                     /locus_tag="ACZPAX_08040"
     tRNA            complement(3205..3280)
                     /locus_tag="ACZPAX_08040"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3245..3247),aa:Phe,seq:gaa)
BASE COUNT          729 a          919 c          981 g          702 t
ORIGIN      
        1 gcccgagttt ttcagtcgct tgaaaaacgg ctcgctgcgg cccagttcaa cgccgcgggc
       61 ctgctgcgcc tggctgatgc tatggtagtc acgctggata tccgggatgt accgcagggc
      121 aatcgccacc gagtagccga ccttgtagga aatgccgatg ctgttgaggc tggcggcaaa
      181 ctcgctgggg ttcgtggccg aaataaagag gatagccacc ggcagcgcca caaaatattt
      241 cagcgaaatg ttgaacatat aaaacagctg ctcagcggtc agatcatacc gccagaacag
      301 atggcacagc accgtgcggg tgccatagag cgtcgtgccc tggttggggt caaacaaaaa
      361 gatgaacaaa tcgttcagcg ccaggaacac cagcataaag accagcatga accggacttc
      421 ccgcagcttg atgtggctta gtttgaacgc cgcaaagctg acgaccatca gccccaacag
      481 cacccaggta ttgtaggtga tcatgccggc gaaggtgaac agcagaaaga acgccagctt
      541 ggttgttcct gtcagctcat gcaccacgct tttacgcggc aggtagttca aaacggtatt
      601 agcagccatg cgcacgcacc tccctgtcag ccgcgataaa gcgttctaca aaatcttccg
      661 cgggcgcaat gccacagcgg ttggccagcg tgtacaggct ggtttctttc agtgccgcct
      721 gctcgatgag ctttgggtcg cagaggatgg ccgccgcgct gcaatccgca atcagccgcc
      781 cgtcacagaa ggccagcgct cggggcgtgt attccagcat caggtgcata tcgtgggtaa
      841 tcatcacgac agtcacaccc ttggcattca gcccgcgcag gaactccata atgtccgtat
      901 aatggcggaa atcctgtccg gctgtcggct catcgaggat gataagctcg gggtcctgca
      961 ccagcacgct ggcgatggtc acacgctttt tctggccgaa gctcaatgca gaaatcggcc
     1021 agttgcggaa gggatacagg ccgcagatct tgagggtgtc ctcgactttg gcgcgggtgt
     1081 cagcctcgct caggccgctg ccctgcagac ccagcgcgac ttcatcgaaa atcatcgttt
     1141 tggaaatcat ctggttcggg ttctgcataa cgtagccgat gtgcctggcg cggcggcgga
     1201 tgttttcttc tttcaagttc ttgccgtcaa agaaaatttc accgctgtcc ggcgtctcaa
     1261 aaccgcagat cagtttggag agggtcgact tgcccgcgcc gttgcgaccc acgatgctga
     1321 ccatctctcc cctgccaatc gtgaaactta cattggtcag ggtgcgctgg cccttgttgt
     1381 agccgaagct caggcccttg acttccagca gcggctgtgg tgcgggcttg ggggcaggca
     1441 gcggctcagc cttgaaccag gcctgcagtt ttgcggtgtc ggcgtcgtcc agtacgacag
     1501 aatcaatgtg agcagggtgc ttttcaggcg tgatggcaac gcccgcatac cgtatagccg
     1561 tgacgtagag cggctcgcgg atgccgtttt cggccagcag tgcgccggac agcagctcgt
     1621 cggggcgcag gtccgccaaa atgcggccgt cgttgaccag tacgatgcgg tccacgctgc
     1681 gccagagcac atcctcgagg cggtgctcga tgatgagcac cgtcgtgtcg gtcttttgct
     1741 ggattgtgtc gatcagttcg atggcctgct tgccggtggc cgggtcgagg tttgccagcg
     1801 gctcgtcaaa gagtaggatt ttgacttcat cgaccatgac acccgccaga ctgacgcgtt
     1861 gcttctgccc gcccgaaagc tcgtgcgggg cgtaatccag gtggttttcg attcccacca
     1921 gttcagcggc gcggcgggtc gtctcgtgca tttctttctg cggggtgcag ctgttttcca
     1981 gcgcaaaggc gatatcttcc gccaccgtca ggccgatgaa ctggccgtcc ggatcctgca
     2041 gcacggtgcc gacatgatcc gccagctcaa aaatgctgct gttgggcgca tccacgccgt
     2101 ctactgtcag gctgcctgta cactcaccgg ggttggagaa gggattcagg ccattgatgc
     2161 aggctgccag tgtgcttttg ccgctgccgg aaggcccggc aatcaggacg cgctcacccg
     2221 ggtagatatc caggttgata tcatgcaggg tgggctgctt ttgggcgcgg tactggaacg
     2281 aaaaattttt gaaggatatg atgggtgttt tggtctctgc catacggcac ctcgcaggga
     2341 aatagttgtt gtaacagcaa aatgccttcc cgcgttcgtg ggaaggcata cgggggtcaa
     2401 acgtgattac tctttgtcca gagagccctt cttggcaatg gtcttggtgt aagccagaat
     2461 cagcaggccg ccgaccacga cagccgtgat gatgttggac acagcggcga aagcgccttg
     2521 tgcaaagacc ttcttaacag gctcagaata aatcaggatg tccagcacag gcgcaacgac
     2581 gatccaggca atggcgtggg ccacaacgtt ggcaacggcg aagatagcgg ccttagcctt
     2641 gttgaagtca ccatcctgaa tgttcagctt cttggcaaac aggccaacga caacaccggt
     2701 gaaggcagag gcaatgaccc agctccacca cgggatgccg ccccaggaca ggtcgataag
     2761 ggcgtgaccg atgaagccga tcaggccgcc ggccacaggg ccgtacatag cagccagcag
     2821 acccagaaca gcgtactgaa ggctgatgtt agtgttgggt accgggctgg ggatcgctac
     2881 gaaacggccc aggacaaaga acagggctgc gccgatgccg acggcaacga aagttttaat
     2941 ggtattgttt ttcatgattc cgcactcctt ttttaatata ccgcaaatct tccctgcttt
     3001 tgtcagggaa cagcttcagt atacctttat agagaggccg cgtcaatggg caaaaggcag
     3061 ggtatgcact atttttgccg cattggggca caaaaaaacc accccgaagg gtggttacgc
     3121 tgtggagcgg ccgacgaggc tcgaactcgc tacctccacc ttggcaaggt ggcgctctac
     3181 cagatgagct acggccgctt aaaatggtgc ctccgatcgg aatcgaacca atgacacggg
     3241 gattttcagt cccctgctct accgactgag ctacagaggc aagcaacgga tataattata
     3301 cacgatggag atgggtttgt caaggagaat c
//
LOCUS       DBMDSQ010000227         3324 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_6468_length_3324_cov_1.778778, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000227 DBMDSQ010000000
VERSION     DBMDSQ010000227.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 3324)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3324)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3324
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6468_length_3324_cov_1.778778"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1247
                     /locus_tag="ACZPAX_08045"
     CDS             <1..1247
                     /locus_tag="ACZPAX_08045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113885.1"
                     /translation="PLVLAGLYGIYTRDKPRFADWLPMSLGMAAMVQSHLLSCELTAL
                     LLILFCLLRLRETLRPARLLAWVKAALLAVGLSAWYLVPFFLSTSSIPFMVNGPLIGK
                     IQFQGLYLIQLFNPFYNGFAGADAGTNPDMMLSLGLPLILGLGLVVFCLLRRRDAQGQ
                     AASILTTAAGFFALTVFLALHIFPWDYVQTTLGRTAGKLAGLFQYPWRFLSLATVLLC
                     LAVIMAVKLLTARDRRLGLTATVVLAAAALFMTGAMQAQMLNGQREVSYNVFSYENPT
                     SCIGVGEYLIDGTSSYETIWAQPKPGSDELHLVSYEKKQGVAYVSVQNDGGEATISLP
                     IFNYGSYCAADENDQPFTITSGENERIVLTIPANYTGTVRVWYQAPGFWRVFEVISAL
                     SLLGTVGYSVFARRKRRVAVTV"
     gene            1263..1901
                     /locus_tag="ACZPAX_08050"
     CDS             1263..1901
                     /locus_tag="ACZPAX_08050"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015554877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - tRNA queuosine(34) biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HIR1113886.1"
                     /translation="MPNRINYQLEMEKVLRTLDGTRPKLLLHACCAPCSSATLERLAA
                     HFDITILYYNPNIYPPEEYHRREAELERFVEEAGYHYPVVELPYDPQEFYTAVKGLES
                     EPEKGARCTVCYRLRLEQAARYAAAHGFDWYCTTLSISPMKDPIRLNDLGTELGKQYG
                     VPFLPSEFRKKDGYKRSLQLSAEYGLYRQDYCGCVFSKKERERPSAPANVIP"
     gene            1917..3215
                     /locus_tag="ACZPAX_08055"
     CDS             1917..3215
                     /locus_tag="ACZPAX_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007045852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease"
                     /protein_id="HIR1113887.1"
                     /translation="MTFYQIAFLFFTYSFLGWVGEVVFTAAVRRKYQDRGVLSGPLCI
                     LYGIGGLVITFALGDMSRDGWFFLFVFSAVYATVIEWIGGHILERTSHTRWWDYSAMP
                     FNLDGYICLGASATWGVLGVVLLKWGNPLLLALFSLIPHGLAAVALWASLVVLGIDLV
                     GTLLAMAGLRYKWPAAEAIENRLANMTVRGGMWILDRVERRMAKAHPALTFARPERQQ
                     SGTFAAGCSPYKIILLFFIGAFLGDITETIFCRVTGGEWMSRSSVVWGPFSIVWGLAI
                     ALVTQLLYRYKDKPASWLFVTGTLLGGAYEYLCSVFTEVVFGAVFWDYSAIPFNLGGR
                     INLLYCFFWGFAAVAWFKVLYPPISAAIEKLPRRFGTVLTWALCVFMAADMVVSSAAL
                     VRYNDRLNGVPAANSIETYLDEHYDNDRMYKVYPKAVHTG"
BASE COUNT          564 a         1021 c         1023 g          716 t
ORIGIN      
        1 tcccgctggt gctggcgggc ctgtacggca tctacaccag ggacaagccc cgctttgccg
       61 attggcttcc catgtcgctg ggcatggcgg ccatggtgca gagccatctg ctctcctgcg
      121 agttgacggc gctgctgctc atcctcttct gcctgctgcg cctgcgggag acgctgcgcc
      181 ccgcgcggct gctggcctgg gtcaaggccg cactgctggc cgtggggttg agcgcgtggt
      241 atctggtgcc gttcttcctc tccacttcgt cgattccctt catggtcaat ggccctctga
      301 tcggcaaaat tcagttccag ggcctctacc tgatacagct gttcaacccc ttctacaacg
      361 gctttgcggg cgcagatgcg ggcacgaacc ccgacatgat gctttcgctg gggctgccgc
      421 tgattctggg gctggggctg gtcgtgttct gcctgctgcg ccgccgcgac gcccagggcc
      481 aggccgcgtc catcctgacg acggccgccg ggttctttgc gctgacggtg tttttggcgc
      541 tgcacatctt cccctgggat tacgtgcaga ccacgctggg ccgcacggcg ggcaagctgg
      601 ccgggctgtt ccagtatccg tggcgtttcc tctcgctggc gacggtgctg ctctgcctgg
      661 cggtcattat ggccgtcaag ctgctgacgg cgcgtgaccg ccgcctgggg ctgaccgcca
      721 ccgtagtgct ggccgctgcc gccctgttta tgaccggggc catgcaggcg cagatgctca
      781 acggccaacg cgaggtcagc tacaatgtgt tcagctacga aaacccgacg agctgcatcg
      841 gcgtggggga gtacctcatc gacggcacca gctcttacga gacgatctgg gcgcagccca
      901 agccgggcag cgatgagctg cacctggtca gctatgagaa aaagcagggc gtcgcctacg
      961 taagcgtaca gaacgacggc ggcgaggcaa ccatttcgct gcctatcttt aactacggca
     1021 gctactgcgc ggccgacgaa aacgaccaac cctttaccat caccagcggc gagaacgagc
     1081 gtattgtgct tacgatcccc gccaactaca ccggcactgt gcgtgtctgg taccaggccc
     1141 ccggcttctg gcgcgtgttc gaggtcattt ccgccttgag cctgctgggc acggtggggt
     1201 acagtgtgtt cgcccgccgc aagcgccgcg tggccgttac cgtgtaaaaa cgaggtctct
     1261 tcatgccaaa ccgcatcaac tatcagctgg aaatggaaaa ggtcctgcgc acactggacg
     1321 gcacgcggcc aaagctcctg ctgcacgcct gctgtgcgcc ctgttccagc gcgacactgg
     1381 aacgcctggc cgcgcatttc gacatcacga tcttatacta caaccccaac atctacccac
     1441 cggaggaata ccaccgccgg gaggccgagc tggaacgctt tgtggaggag gcgggctacc
     1501 actacccggt ggtggagctg ccctacgacc cgcaggagtt ttacaccgcc gtgaagggac
     1561 tggagagcga gccggagaag ggcgcacgct gcacggtctg ctaccggctt cggctggaac
     1621 aggccgcccg ctatgccgcc gcgcacggct ttgactggta ctgcacgacg ctgtccatct
     1681 cgcccatgaa ggaccccatc cgcctgaacg acctgggcac cgaactgggc aagcagtacg
     1741 gcgtgccgtt tttgcccagt gagttccgca aaaaggacgg ctacaagcgc agtttacagt
     1801 tgagcgccga gtatgggttg taccggcagg attactgcgg gtgtgtattc agcaaaaaag
     1861 aaagagaacg tccctccgcc cctgcaaacg ttatccccta aacaggagtt acctttatga
     1921 cattctacca aatcgcattt ttatttttca cttactcgtt cctgggctgg gtgggggagg
     1981 tcgtctttac ggcggcggtc cgccgcaagt accaggaccg gggcgttctg agcggcccgc
     2041 tgtgcatttt gtacggcatc ggcggtctgg tcatcacgtt tgctttgggg gatatgagcc
     2101 gcgacggctg gttcttcctc tttgtgttca gcgccgtcta tgccaccgtc attgagtgga
     2161 tcggcgggca cattttggag cgcacctccc acacccgctg gtgggattac agcgctatgc
     2221 cgttcaacct ggacggttac atctgcctgg gtgcatcggc cacctggggc gtgctgggcg
     2281 ttgtgctgct caagtggggc aacccgcttt tgctggcgct gttcagcctg atcccccatg
     2341 gcctggccgc cgtagcgctg tgggcatcgc tggtggtgct gggcattgat ctggtcggca
     2401 cgctgctggc tatggcgggc ctgcgctaca agtggcccgc cgccgaggcc atcgaaaacc
     2461 gccttgccaa catgacggtg cgcggcggta tgtggatcct ggatcgtgtg gagcgccgta
     2521 tggccaaagc ccacccggcc ctgacctttg cccgccccga gcggcagcag agcggcacct
     2581 ttgccgccgg gtgcagcccc tataaaatca ttctgctgtt cttcatcggc gcattcttag
     2641 gcgatatcac cgagaccatt ttctgccgcg tcacgggcgg cgagtggatg agccgttcca
     2701 gcgtggtgtg gggcccgttt tccatcgtgt ggggcctcgc catcgcgctg gtcacacagc
     2761 tgctctaccg ctacaaggac aaacccgcca gctggctgtt tgtcaccggc acgctgctgg
     2821 gcggcgccta tgagtacctg tgcagcgtct ttaccgaggt cgtgttcggc gcggtgttct
     2881 gggattacag cgcgataccg ttcaatttgg gcgggcgtat caacctgctg tactgcttct
     2941 tctggggctt tgcggcggtg gcgtggttca aagtgctgta cccgccgatt tctgccgcca
     3001 tcgaaaagct gccccgccgc ttcggcacgg tgctgacctg ggcgctctgc gtgttcatgg
     3061 cggcggatat ggtcgtcagc tcggctgcgc tggtgcgcta caacgaccgc ctgaacggcg
     3121 ttcccgccgc gaacagcatc gaaacttatt tagacgagca ctacgacaat gaccgaatgt
     3181 ataaggtgta cccgaaggcg gtgcatacag ggtaattgca aaaatctttg tatattccgc
     3241 acatatgtgt tataataatt ttgtatctgt tgtataagaa tcgaggaggc ggcgtaggcc
     3301 ttgtagctgc gtccggcctt gagc
//
LOCUS       DBMDSQ010000228         3298 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_6537_length_3298_cov_1.586175, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000228 DBMDSQ010000000
VERSION     DBMDSQ010000228.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 3298)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3298)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3298
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6537_length_3298_cov_1.586175"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="ACZPAX_08060"
     CDS             <1..363
                     /locus_tag="ACZPAX_08060"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005930056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:0051991 -
                     UDP-N-acetyl-D-glucosamine:N-acetylmuramoyl-L-alanyl-D-
                     glutamyl-meso-2,
                     6-diaminopimelyl-D-alanyl-D-alanine-diphosphoundecaprenol
                     4-beta-N-acetylglucosaminlytransferase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0030259 - obsolete lipid glycosylation
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HIR1113888.1"
                     /translation="VVTEYINNMPQLLAAADLVIARSGALTLAELEAVGRASVLIPSP
                     NVAENHQYYNALELQKAGAAVVIEEKNLTGDGLVKTVEELLTTPGKLAEMGANAKTLG
                     NPHSLDMITEKLLELVKS"
     gene            462..1613
                     /locus_tag="ACZPAX_08065"
     CDS             462..1613
                     /locus_tag="ACZPAX_08065"
                     /inference="COORDINATES: protein motif:HMM:NF020067.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HIR1113889.1"
                     /translation="MDRNQRSRRQKSVSGSYSQAPKRSMGSRMGLEKPGTPRQQPPKK
                     SSPRSQRPTQQINPQKAGYDPAAPKRQRRVTQAEIIRKRNRRRAMSIMAVLAVLAVGA
                     FVSLNLLFKVAFFRVENFDRTTPADTGIYTSDELINALNIEKNSNLFGFSTAEKTRQL
                     AQQFPYLEQVQVEVQMPATVVVKVQPAVERFACMYSGGWMVLSDSLKILRTDVNQPDG
                     LILLSIALPTDFAPTVGQNITPESYNSLLGGEQATAETAGLQASAMQTLTEILDGLQT
                     YNLFDGTTAISVQDLSDIEFTYQNRVQVKLGSETNLAYKLRLAAAALADPEKGLAASD
                     KGVLDISTQQSNGDIRAYFAPDEPPPPPPPPPTTTPEPEEEETLANTAE"
     gene            1717..2217
                     /locus_tag="ACZPAX_08070"
     CDS             1717..2217
                     /locus_tag="ACZPAX_08070"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR1113890.1"
                     /translation="MPLPELEELYTLYRTDLYRYLCHLTHDPTEAEDILSETFLRAMR
                     RLPTYRGDCAVKTWLFGIARNIWLESLRKRRPDVSADDLLENYLVEDTLTEHTDTRLQ
                     WQRVCELLQQKDDRARRIMQLRAEGYTYAEIAVQLHISESSARVIEHRTRSWLKEILT
                     KEGYDL"
     gene            2214..2786
                     /locus_tag="ACZPAX_08075"
     CDS             2214..2786
                     /locus_tag="ACZPAX_08075"
                     /inference="COORDINATES: protein motif:HMM:NF024881.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HIR1113891.1"
                     /translation="MKKLAENAKIPCAVCRDLMPLVQDGIASPESEQLVRAHIAVCPA
                     CQALWQGTEPPAAPELQPPDDTKILKRLRRRYSIHLLLLAFVGIAVGIALGYTGSANW
                     VILFLPLVWSVLTYRDIGMGKPLLLFTLGLAVLDAIIKQMALLESFAPGNFLANLVSG
                     CILYVGLGGVGWLIGWLLHYAKESTHENKG"
     gene            2770..>3298
                     /locus_tag="ACZPAX_08080"
     CDS             2770..>3298
                     /locus_tag="ACZPAX_08080"
                     /inference="COORDINATES: protein motif:HMM:NF051836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfjL-like protein"
                     /protein_id="HIR1113892.1"
                     /translation="MKTKAKRTKTMRRFALTAALLLILALLLAFDAINGDPLSQQWAI
                     HRAIRYAEKLYPEETFHWDQRTAADGQFFHYVVYVQSDQSSDTNFAVQTRFWLLTDDE
                     IRGKELEPDHVFCVDHRWNTAYRLGDEAAQQAAAYLTVQAPELNFASVYGAKGNTVEI
                     DLCYTEQDGMTPANYV"
BASE COUNT          755 a         1005 c          933 g          605 t
ORIGIN      
        1 gttgtgaccg agtacatcaa caatatgccc cagctgctgg cggcggcgga tctcgtcatt
       61 gcgcgttccg gtgcgctgac gctggccgaa ctggaagccg tgggccgcgc gtcggtgttg
      121 atccccagcc ctaacgtggc cgaaaaccac cagtattaca acgcccttga actgcaaaag
      181 gctggggcag ccgtggtcat tgaggaaaag aacctgaccg gtgacggcct ggtaaagacc
      241 gtggaagaac tgctgaccac cccaggcaag ctggccgaaa tgggagccaa cgccaagaca
      301 ttaggcaacc cccacagcct ggatatgatt acggaaaaat tgctggagct ggtaaaatca
      361 taacagccgc agggggcggc atcctcgacg ccccgtagcc gccactgccc catagggcag
      421 ccggcacgca cctataaaaa tgtattggaa aggagccgcc catggatcga aaccagcgca
      481 gccgcaggca aaaatccgtg agcggctcct attcgcaagc tcccaaacgc agcatgggca
      541 gccgtatggg gctggaaaaa ccgggcaccc cacgccaaca gccgccgaaa aaatcctcgc
      601 cgcgcagcca gcgccccacc cagcagatca acccgcaaaa agccgggtac gaccccgccg
      661 cgcccaaacg ccagcgccgc gtaacccagg cagaaatcat ccgaaaacgc aaccgccgcc
      721 gtgccatgag tatcatggcg gtgctggccg tactggccgt aggggctttc gtctcgctta
      781 acctgctgtt taaagtggcc tttttccggg tcgaaaactt cgaccgcacc acccccgccg
      841 acaccggcat ctacacctcg gacgagctta tcaacgcgct gaatatcgag aagaactcga
      901 atcttttcgg cttctccacc gcagaaaaga cccgccagct ggcccagcag tttccgtacc
      961 ttgagcaggt gcaggttgag gtccagatgc ctgctactgt cgtggtcaag gtgcagcccg
     1021 ccgtggagcg attcgcctgt atgtactccg gcggctggat ggtgctgagc gattccctca
     1081 agatcctgcg caccgacgtc aaccagcccg acggcctgat tttgctctcc atcgcgctgc
     1141 ccacagattt tgccccaact gtcggccaga acatcacgcc ggaaagctat aactcgttgc
     1201 ttgggggcga acaggccacc gccgagacgg caggattgca ggcgtcggcc atgcagacct
     1261 tgaccgaaat actggacggc ttgcagacgt acaacctgtt tgacggcacc acagccatta
     1321 gtgtgcagga tttgagcgat atcgagttca cctaccagaa ccgtgtgcag gtcaagttgg
     1381 gcagcgagac gaatcttgcc tataaactgc gcctggctgc tgccgcactg gccgaccccg
     1441 aaaagggcct ggccgccagc gataagggtg tgctggatat ctcgacccag cagtccaacg
     1501 gcgacatccg cgcctacttt gcccccgacg aacccccccc cccccccccc cccccgccta
     1561 ccaccacgcc ggaaccggag gaagaggaaa ccctggctaa cacagcagaa taaaccagcg
     1621 ccccgacaaa agtcggggct tttttgtagt tgctgtaacg tttggggcaa aatcatcact
     1681 tatagagtga gagcacagag gaaaggagag caggccatgc cgctgccgga actggaagaa
     1741 ctgtatacgc tataccgtac ggacctgtac cgatacctct gccacttgac acacgaccca
     1801 accgaggctg aggacatact gagcgagacc ttcctgcgtg ccatgcggcg gctacccacc
     1861 taccgcggcg actgcgctgt caaaacatgg ctgttcggca ttgcccgcaa catctggctg
     1921 gaaagtctgc gtaagcgccg ccccgacgtc agcgcagatg acctgctgga aaactacctg
     1981 gtcgaggata ccctgaccga gcataccgac acacgcctgc aatggcagcg ggtctgtgaa
     2041 ctgctgcagc agaaagacga ccgcgctcgc cgcattatgc agctgcgcgc ggagggctat
     2101 acctatgcgg aaatcgcggt acagctgcat atcagtgaaa gctcggcccg cgtcatcgaa
     2161 caccgcaccc gcagctggct gaaggaaatt ttgacgaagg agggttatga cctgtgaaga
     2221 aacttgctga aaacgcaaaa atcccctgtg cggtctgccg ggacttgatg ccattggtgc
     2281 aggacggaat cgccagtccg gaaagtgagc agcttgtccg cgcccacatt gccgtctgtc
     2341 cggcctgtca ggccctgtgg caggggacag agccgcccgc agcgccggaa ctgcagcccc
     2401 ccgacgatac caaaatactc aagcggctgc gccgccgtta cagcatccac ctgttgctgc
     2461 tggcctttgt aggcattgca gtgggcatag ccctggggta tacaggcagc gcgaattggg
     2521 tcattctgtt tttgccgcta gtctggagtg ttctgaccta ccgtgacatc ggcatgggaa
     2581 agccgctgct gctgttcacg ctggggctgg cggtgctgga tgctatcata aagcagatgg
     2641 ccttgctgga atcgttcgca cctggtaatt tcttggcaaa tttggtgtcc ggctgcattt
     2701 tgtacgtcgg tttgggcggc gttggctggc tgatcggctg gctgctgcac tatgcaaagg
     2761 agagtaccca tgaaaacaaa ggctaaaaga acgaaaacca tgcgccgctt tgcgctgaca
     2821 gcagccctgc tgctgattct cgcgctgctg ctggcctttg acgccatcaa cggtgacccg
     2881 ctcagccaac agtgggctat acatcgggcc attcgatatg cggaaaagct atatccggag
     2941 gaaacatttc actgggacca gcgcaccgcc gctgatggac agttcttcca ctatgtggta
     3001 tacgttcagt ctgaccagag cagtgacaca aactttgcag tgcagacgcg gttctggctg
     3061 ctgactgatg atgaaatccg gggtaaggag cttgagccag accatgtgtt ctgtgtagac
     3121 caccgctgga acaccgctta ccgcctgggg gatgaagctg cccagcaggc agccgcgtat
     3181 ctgaccgtgc aggcccccga attaaacttt gcatctgtct acggcgcaaa gggcaacacg
     3241 gtagagatcg acctttgcta taccgagcag gacggcatga ccccggctaa ctatgtgg
//
LOCUS       DBMDSQ010000229         3281 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_6582_length_3281_cov_1.997484, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000229 DBMDSQ010000000
VERSION     DBMDSQ010000229.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 3281)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3281)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3281
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6582_length_3281_cov_1.997484"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1437
                     /locus_tag="ACZPAX_08085"
     CDS             <1..1437
                     /locus_tag="ACZPAX_08085"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR1113893.1"
                     /translation="FMLDVLRRCQTVACLGYQGYRYVQHSGSTMAVAGVARNYLERPE
                     YLLEWSRYFAEKGNYLRAEGLLNDAIENLSQKENFDLSTQWQQARYHELRHSCAEVYR
                     LLSRRTGRESMLLRAGLIHLGLPVYLAFLRERRSSAQTPPAAPQWRTPAPPTPVPAVS
                     IIIPVYKVERYLDTCVQSVLDQTFQDIEVLLVDDGSPDRCPEMCDAWAKSDRRVRVIH
                     RKNGGLSAARNTGIETARGRYIVFVDSDDFLEPDTIRRAVAEQERTGAELVIFNLVYA
                     NDRGERWPTPDFTIFQDEILDEDGVWARYFALENQKIYYTVAWNKLYTAKLFADGLRF
                     PEGKRYEDQFMLPHILHRCQNIACLGYQGYRYVQRADSIMAQGPASHYLDRPEYLLEW
                     CGYFTEKGDYLRAEGLLNDAIKNLAEKDGFDLTTAQQRTRYRQACHDCSTAYGNLSRR
                     TGRESMLLRAGLLRLGLPLYRIFLKHKT"
     gene            1452..2312
                     /locus_tag="ACZPAX_08090"
     CDS             1452..2312
                     /locus_tag="ACZPAX_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006572152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1113894.1"
                     /translation="MEKTQLQKGYHVHYGPAQGWSQVDVSELWRYKDLIWLFVKRDFT
                     VMYKQTVLGPAWILINPLLSTAMYCVMFGTIAHLSTDDVPQILFYLAGTALWGFFSAC
                     INKVSGTFTTNSAIFSKVYFPRLAMPISTMLSGLINFLVQFALFFVLLLVYLAKGEVS
                     PHWGMLLLTVPLVLQVGLLGLGCGIIVSSLTTRYRDLMVVVTFGVQLWMYGSPVVYPL
                     SMIDNRLLRAIMIVNPMTAPMESFRYIFFGTGQVNGLMWVLSLAWTAVLLVVGLSLFS
                     KVEKTFVDTV"
     gene            2325..>3281
                     /locus_tag="ACZPAX_08095"
     CDS             2325..>3281
                     /locus_tag="ACZPAX_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007045744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1113895.1"
                     /translation="MSENELMIRVQDVKKQYRLGQIGGGTLRSDLQSWWARKRGKEDP
                     NSLIGTDQRLIGTTFMALNGVSFTVKKGEAVGIIGSNGAGKSTLLKLLTHVTAPSAGD
                     IDLYGRVASMLEVGTGFHPEMTGRENVYLNGAILGMTHAEIDAKMDEIIAFSEVGDFI
                     DTPVKRYSSGMYVKLAFSVAAHLDSEIMIMDEVLAVGDMKFQKKCLDKMRQAAQRDGK
                     TVLYVSHNMSTIRDLCDRCIVLDKGKVIFDGDVDEGIATYLSTRWQTSDFVDYTNHKR
                     DNWFKRDNIRIQNIHVQVQSVEAIERTQPVRIHYVLKANELAE"
BASE COUNT          643 a         1000 c          984 g          654 t
ORIGIN      
        1 tttatgctgg atgtgctgcg ccgctgccag acggtggcct gcctgggcta ccaggggtac
       61 cgctacgtgc agcacagcgg cagcaccatg gctgtggccg gtgtggcccg caactacctg
      121 gaacgccccg agtatctgct ggaatggagt cgctattttg ccgagaaagg caactacctc
      181 cgggccgagg gccttttgaa cgacgccatc gagaacctgt cccagaagga aaacttcgac
      241 ctgtccaccc agtggcagca ggcgcggtat catgagctgc gtcacagctg cgccgaggtt
      301 taccgactgc tctcccgccg caccgggcgg gaaagtatgc tgctgcgcgc cgggctgatc
      361 cacttagggc ttccggtcta cctggccttc ctgcgggagc ggcgcagcag cgcccagacc
      421 ccgcccgccg cgccccagtg gcgaaccccg gccccgccca ccccggtccc ggcggtcagt
      481 atcattatcc cggtgtacaa ggtcgagcgg tatcttgaca cctgtgtgca gagcgtgctg
      541 gatcagacct tccaggatat tgaggtgctg ctcgtcgacg acggcagccc cgaccgctgc
      601 cccgaaatgt gcgatgcctg ggcgaaaagc gaccgccgcg tgcgggtcat ccaccgcaag
      661 aacggtgggc tttccgctgc gcgcaacacc ggcatcgaga ccgcccgcgg gcggtacatc
      721 gtctttgtgg acagcgacga ctttctggag ccggatacca tccgccgcgc tgtggccgag
      781 caggagcgca ccggcgcgga gctggtcatc ttcaacctcg tctacgccaa tgaccgcggc
      841 gaacggtggc ccacgccgga cttcacgatc tttcaggatg aaatactcga cgaggacggc
      901 gtgtgggcgc ggtatttcgc gctggaaaac caaaaaatct actataccgt cgcttggaac
      961 aagctgtaca ccgccaagct ttttgcggat ggcctgcgat ttcccgaggg caagcggtac
     1021 gaggatcagt tcatgctgcc ccacatcctg caccgctgcc agaacattgc ctgcctgggc
     1081 taccagggct accgctatgt gcagcgggca gacagcatca tggcgcaggg cccggccagc
     1141 cactacctgg accgccccga atacctgctg gaatggtgcg gctactttac cgagaaaggc
     1201 gactatctgc gggccgaggg cctgctgaac gacgccatca agaacctggc cgagaaggat
     1261 ggctttgacc tgaccacggc gcagcagcgc acccgctacc gccaggcatg ccacgactgc
     1321 tctacggctt acggcaattt gtcccgccgc accgggcggg agagtatgct gttgcgcgcc
     1381 gggcttttgc gcctgggcct gcccctgtac cgaatcttcc tcaaacacaa aacctaagga
     1441 gttcccctac aatggaaaaa acacaactgc aaaaaggata ccatgtccac tacggccccg
     1501 cgcaggggtg gagccaggtc gatgtaagcg aactgtggcg ctataaggat ttgatctggc
     1561 tctttgtcaa gcgcgatttc accgtcatgt acaagcagac cgtgctgggc cctgcgtgga
     1621 tcctgattaa cccgctgctg tccacggcca tgtactgcgt tatgttcggc accatcgccc
     1681 acctgtccac cgacgatgta ccgcagattt tgttctacct ggccggtacg gcgctgtggg
     1741 ggttcttctc cgcctgcatc aataaggtgt cgggcacgtt taccaccaac tccgctattt
     1801 tcagcaaggt gtatttcccg cggctggcga tgcccatttc cacgatgttg tcaggcctta
     1861 tcaatttcct ggtgcagttt gcgctgttct ttgtgctgct gctcgtctac ctggccaagg
     1921 gtgaagtcag cccccactgg gggatgttgc tgctgacggt gccgctggtg ctgcaggtgg
     1981 gtctgctggg cctgggctgc ggcatcatcg tgtccagcct gactacccgt taccgtgatt
     2041 tgatggtcgt ggtgaccttt ggcgtgcagc tatggatgta tggttccccg gtggtctatc
     2101 cgctgtcgat gatcgacaac cgactgctgc gggccattat gatcgtcaac ccgatgaccg
     2161 ccccgatgga gagcttccgt tatatcttct tcggtaccgg gcaggtcaac gggttaatgt
     2221 gggtgctttc gctggcctgg accgccgtgc tgctggtcgt gggtctgtcg cttttcagca
     2281 aggtcgaaaa gaccttcgtc gatacggtgt aagggggtcc tgacatgagt gaaaatgaac
     2341 tgatgatccg tgtacaggac gtcaaaaagc agtaccgcct gggccagatc ggcggcggca
     2401 cgctgcgcag tgacctgcaa agctggtggg cccgcaagcg cggcaaagag gacccgaaca
     2461 gcctgatcgg caccgaccag cgcttgatcg gtactacatt tatggcgctg aacggcgtca
     2521 gcttcaccgt caaaaagggc gaggccgtgg gtatcatcgg ctccaacggc gcgggcaaat
     2581 ccacgctgct caagctgctg acccacgtca cggcccccag cgccggtgat atcgacctgt
     2641 acggccgtgt ggcctcgatg ttggaggtcg gcaccggctt ccaccccgaa atgaccggcc
     2701 gcgagaacgt ctacctgaac ggcgcgattt tgggcatgac ccacgcagaa attgacgcca
     2761 agatggatga gatcattgcc ttctccgagg tcggcgactt catcgacacg ccggtcaagc
     2821 ggtattccag cggtatgtat gtcaagctgg cgttcagcgt ggcggcccac ctggacagtg
     2881 aaatcatgat catggacgag gtcctggccg tcggcgatat gaagttccag aaaaagtgtc
     2941 tggataagat gcgccaggcc gctcagcgcg acggcaagac cgttttgtac gtcagccaca
     3001 atatgtccac gatccgcgat ctgtgcgacc gctgcatcgt gctggacaag ggcaaggtta
     3061 tctttgacgg tgacgttgac gagggcattg ccacctacct gtccacccgc tggcagacgt
     3121 cggattttgt ggactacacc aaccacaagc gcgacaactg gttcaagcgc gacaacatcc
     3181 gcatccagaa catccatgtg caggtgcagt cggtggaggc aatcgagcgc acccagcctg
     3241 tgcgcatcca ttacgtgctg aaagccaacg aactggccga g
//
LOCUS       DBMDSQ010000230         3262 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_6629_length_3262_cov_1.527997, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000230 DBMDSQ010000000
VERSION     DBMDSQ010000230.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 3262)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3262)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3262
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6629_length_3262_cov_1.527997"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..239
                     /locus_tag="ACZPAX_08100"
     CDS             <1..239
                     /locus_tag="ACZPAX_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta-related
                     protein"
                     /protein_id="HIR1113896.1"
                     /translation="KAVAVVLQTNMQRACKQLTKVELFDIYRGEKLGADKKSMAFSLF
                     FQPGDKALTPDEVDRFVKKILGNLKFKLGIEIRE"
     gene            368..1339
                     /locus_tag="ACZPAX_08105"
     CDS             368..1339
                     /locus_tag="ACZPAX_08105"
                     /inference="COORDINATES: protein motif:HMM:NF016119.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase domain-containing protein"
                     /protein_id="HIR1113897.1"
                     /translation="MAKIHRNDAPLYQGQPANPYSTATLPEIPAQKNAAEFYDQDHDE
                     EFTEREEKKHPKKHAQKNAEARKKKRGDLVYTIAMVVFAAIFLVSGGLLVKRFIDDKK
                     TESEFADLQSMIDTSATPEPAAAGENVNPNGAKFAALKDKNTDFIGWISIDGTTLDFP
                     VMYAPDNKDFYLRHDFNKQYSVYGVPYLDEKTTLGANAESGNLIIYGHNMKTGTIFGC
                     LTEYKKAAYYQEHPYIEFDTLYGDAKYEVFAAFAIDVVNDTSFVYNQYVDMDENTYNA
                     YVDEVLSRSDVDSGIRPSYGEQLLTLSTCEYSTDNGRYVVVARRVSS"
     gene            1709..>3262
                     /gene="glmS"
                     /locus_tag="ACZPAX_08110"
     CDS             1709..>3262
                     /gene="glmS"
                     /locus_tag="ACZPAX_08110"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HIR1113898.1"
                     /translation="MCGIVGFTGAAQAAPILLDGLAKLEYRGYDSAGLAVQNAAGKIE
                     VVKAKGRLKVLSEMTDGGSAVSGSCGIGHTRWATHGEPSVINAHPHYSRDQKIAVVHN
                     GIIENYLEIKERLLNKGFTFVSQTDTEVVAQLLDYYYTGESAGNALDAITRMMVHVRG
                     SYALGVLFADEPGVIYAVRKDSPLIVGKCGDGAIIASDVPALLKYTRTVYYIDNLEIA
                     RLTPDSIDFFNIDKEPITRESTTIEWDAEAAEKGGYEHFMMKEIYEQPAAVRDTISPR
                     LKDGQIDLSELALDEAAIQNVRRLYIIGCGSAYHVGMAARYVFESLARIPVEVDVASE
                     FRYRNPVLESDSLAMVISQSGETADTLAALRLCKERGVRTIGIVNVVGSSIAREADAT
                     MYTWAGPEISVATTKAYSTQLAACYLLATEFARVRGTLADGQYEHLVTELEALPEKIE
                     KTLADKERIQWFASKYANAKDAFFIGRGLDYAVALEGSLKFKEISYIHSEAFAAGEMK
                     HGPISLVENG"
BASE COUNT          773 a          951 c          882 g          656 t
ORIGIN      
        1 gcaaagctgt tgcagtggta ctgcaaacta atatgcagcg cgcctgcaag cagctgacca
       61 aggtcgagct gttcgacatc taccgtggtg aaaaactcgg cgctgacaaa aagagcatgg
      121 cgttcagcct gttcttccaa cccggcgaca aggccctgac ccccgatgag gttgaccgct
      181 tcgtcaagaa gattctcggc aacctcaagt tcaagctggg catcgagatc cgcgagtaat
      241 cctgtatcat acaaaccgct ctgtgccgct tggcgcaggg cgttttttcg cggaatttcg
      301 cttgcatttt ttctggttcc gtcgtatagt aaatagagac tatccagact gtcacaggag
      361 aatgattatg gcaaaaatac accgcaatga cgcgccgctg taccagggcc agcccgcgaa
      421 tccatacagc accgccactc tgccggaaat tcccgcccag aagaacgcgg ctgaatttta
      481 cgaccaggac cacgatgagg aatttaccga gcgcgaggag aaaaagcacc ccaaaaagca
      541 cgcccagaag aacgcggagg cccgcaagaa aaagcgaggc gatctggtct atacgattgc
      601 catggtcgtg ttcgcggcga ttttcctggt cagcggcggg ctgctggtca agcgctttat
      661 cgatgataaa aagaccgaga gcgagtttgc cgacctgcaa tcgatgattg acacctccgc
      721 cacgccggaa ccggccgcag caggggagaa cgtcaacccc aacggtgcca agtttgccgc
      781 gctgaaagat aagaacactg acttcatcgg ctggatttcc attgacggca caacgctgga
      841 cttcccggtt atgtacgcgc ccgacaacaa ggatttctac ctgcgccatg acttcaacaa
      901 gcagtacagc gtctacggcg tgccgtacct ggacgaaaag accacgctgg gtgccaacgc
      961 cgaaagcggg aaccttatca tctacggcca caacatgaag accggcacga ttttcggctg
     1021 cctgaccgaa tacaaaaagg ccgcctacta ccaggagcac ccctacattg agttcgacac
     1081 actttacggt gacgcaaaat atgaggtgtt tgccgccttc gccatcgacg tggtgaacga
     1141 tacgtccttc gtctacaacc agtatgtgga catggacgag aatacctaca acgcctatgt
     1201 ggatgaggtc ctttcccgca gcgatgtgga cagcggcatc cgccccagct acggcgagca
     1261 gctgctgacc ctctccacct gcgaatactc gactgacaac ggccgctatg tcgtcgtggc
     1321 gcggcgcgtc agctcctgat tttcataagt tttacagaac aatacttaaa aatcttttgc
     1381 ggcaagtacc aaacgcaggt aaaattgcgt ttggtacttg ctattctttg tcggatgtgc
     1441 tatcctaata aatgcgcgat aacttttgat tcgttcttat atggtgtaag aaaccggaaa
     1501 gggagaatgc gcgcagcggg cggccgtagc ccttgacggc cgtccgcttt tgtttttgca
     1561 ccgcgtacaa aggcgcaaaa aatgcacaaa agtatagaca aattgcgcaa gaaagcatat
     1621 actatataag tattttgatt ttacgcgcta aagtatatgt ataggaaagt atatcaaaac
     1681 acagttacag taagggagga tacctgctat gtgtggaatc gtaggcttca ccggtgcggc
     1741 acaggccgcg cccattctgc tggatggcct ggcgaaactg gaataccgcg gctatgactc
     1801 tgccggtctg gccgtgcaga acgctgccgg taaaatcgag gtcgtcaagg ccaagggccg
     1861 cctgaaggtt ctgagcgaaa tgaccgacgg cggcagcgcg gtttccggca gctgcggcat
     1921 cggccatacc cgctgggcta ctcacggcga accgtccgtc atcaacgccc acccgcacta
     1981 ttcccgtgac cagaaaatcg ctgtcgtaca caacggcatc attgaaaact acctggaaat
     2041 taaagagcgc ctgctgaaca aaggcttcac cttcgtctcc cagaccgaca ccgaggttgt
     2101 ggctcaactg ctggactact attacaccgg cgagtccgcc ggcaacgcgc tggatgccat
     2161 cacccgcatg atggtccatg tgcgcggctc ctacgcgctg ggcgtgctgt ttgccgatga
     2221 acccggcgtc atctacgccg tgcgcaagga cagccccctc atcgtcggca agtgcgggga
     2281 cggtgccatc atcgcctccg atgtgcccgc gctgctcaag tacacccgca cggtctacta
     2341 cattgacaat ctggaaatcg cccgcctgac accggattcc atcgacttct tcaatatcga
     2401 caaggaaccc atcacccgcg agtccactac catcgaatgg gatgccgaag cagccgaaaa
     2461 gggtggctac gagcatttca tgatgaagga aatctacgag cagcccgccg ctgtacgcga
     2521 cacgatctcg cctcgcctga aggacggcca gatcgatttg tctgagctgg cactggacga
     2581 ggctgccatc cagaatgtac gccgcctcta catcatcggc tgcggctcgg cttaccatgt
     2641 cggcatggcc gcgcgctatg tgtttgagag cctggcccgc atcccggtcg aggttgacgt
     2701 cgccagcgag ttccgctacc gtaacccggt gctggagtcg gattccctcg ccatggtcat
     2761 cagccagagc ggcgagaccg ccgacacgtt ggccgccctg cgcctgtgca aggagcgcgg
     2821 cgtgcgcacc atcggcatcg tcaacgttgt cggttccagc atcgcccgcg aggctgacgc
     2881 caccatgtac acctgggccg ggccggaaat ttccgttgcc accaccaagg cgtatagcac
     2941 ccagttggcg gcctgttatc tgctggctac cgagtttgcc cgcgtgcgcg gcaccctggc
     3001 tgacggccag tatgagcacc ttgtcaccga actggaagcc ctgcccgaga aaatcgagaa
     3061 aacgcttgcc gataaggagc gtatccagtg gtttgccagc aagtacgcca acgccaagga
     3121 cgccttcttc atcggccgcg ggctggacta tgctgttgcg ctggaaggca gcctgaaatt
     3181 taaggaaatc agttatatcc actccgaggc atttgctgcg ggtgagatga agcacggccc
     3241 gatttccctg gtcgagaacg gc
//
LOCUS       DBMDSQ010000231         3257 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_6640_length_3257_cov_1.740177, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000231 DBMDSQ010000000
VERSION     DBMDSQ010000231.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 3257)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3257)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3257
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6640_length_3257_cov_1.740177"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..382)
                     /locus_tag="ACZPAX_08115"
     CDS             complement(<1..382)
                     /locus_tag="ACZPAX_08115"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003469727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HIR1113899.1"
                     /translation="MKTYLVTGCAGFIGSNFVHYMLEKYEDIRLINLDKLTYAGNLEN
                     LQDIEDDARHIFVQGDICDKALVTDLIAKYDPDYVINFAAESHVDRSIKNPEIFVESN
                     VLGTVNLLQCCKDAWYDAAAKTWKE"
     gene            complement(386..1291)
                     /gene="rfbD"
                     /locus_tag="ACZPAX_08120"
     CDS             complement(386..1291)
                     /gene="rfbD"
                     /locus_tag="ACZPAX_08120"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HIR1113900.1"
                     /translation="MKILITGCRGQLGTELQRQLSEEGCAIGPLPERLRKATVIPVDV
                     DELDITDREATINYIRRHQPDTVINCAAFTNVDGCETARDAAFKVNAIGPRNLALACE
                     KIGARLIHVSTDYVFPGTANGGVALDECAVPAPISAYGQTKLLGEQYVERFCRRHFIV
                     RTAWLYSYYGKNFVKTMIRLGRTHDKITVVNDQLGNPTNAVDLAYHILKLAVSHDYGI
                     YHCTGNGICSWYDFASAIMQGAGLSCKVEPVTSAQYAAANPASANRPAWSALENRMLR
                     CTVGDEMRDWQDALKDFFANWNGEL"
     gene            complement(1310..2194)
                     /gene="rfbA"
                     /locus_tag="ACZPAX_08125"
     CDS             complement(1310..2194)
                     /gene="rfbA"
                     /locus_tag="ACZPAX_08125"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015536678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HIR1113901.1"
                     /translation="MKGIILAGGAGTRLYPLTMVTSKQLLPVYDKPMIYYPLSTLMLA
                     GIQDILIISTPTDTPRFEALLGDGSQYGLHLQYKVQPSPDGLAQAFILGEEFIGDDCC
                     AMVLGDNIFYGAGFSKRLKTAAANAANGRSTVFGYYVNDPERFGVVAFDKDGHATDIE
                     EKPANPKSNYAVTGLYFYNNEVVKMAKQVKPSARGELEITTLNQMYLDQGKLDVQLLG
                     RGYAWLDTGTMDSLVEAATFVQTVEQRQGIKISAPEEIAYKYGWITKEKLLESAARYG
                     KSPYGQHLKNVADDKLQY"
     gene            2484..2822
                     /locus_tag="ACZPAX_08130"
     CDS             2484..2822
                     /locus_tag="ACZPAX_08130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113902.1"
                     /translation="MKRFFCAAFFVAVVALLAACGALNVNSAAAQATATPTSSTSRTQ
                     KSDSTVKVNETAAKVDVVTAQSSASPTATAAPAQVETPATGDFPVVYLAGVVGLILIC
                     SAGLLMLRKI"
     gene            2957..>3257
                     /locus_tag="ACZPAX_08135"
     CDS             2957..>3257
                     /locus_tag="ACZPAX_08135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113903.1"
                     /translation="MPRRYRKLRGDTAYYPFAARTGLRARQRRHARLLRVLGTAALAL
                     AAVLLVRQTVFRGGQPTAPAPIAAAQTTAPGQAHVTVAPVAATRHFLINGIAAPEK"
BASE COUNT          603 a          941 c         1006 g          707 t
ORIGIN      
        1 cctctttcca ggtcttggcg gcggcgtcat accaggcgtc cttgcagcac tgcagcaggt
       61 tcacggtgcc cagcacgttg ctctcgacga aaatttcggg gttcttgatg ctgcggtcga
      121 cgtggctctc ggcggcaaag ttgatgacgt aatcggggtc atacttggcg atcaggtcgg
      181 tgacgagcgc cttgtcgcag atatcaccct gcacaaagat gtggcgggcg tcgtcctcga
      241 tgtcctgcaa attctcgagg ttgccggcgt aggtcagctt gtcgaggttg ataaggcgga
      301 tatcctcata cttttccagc atatagtgga caaagttcga gccgatgaaa ccggcgcagc
      361 cggtaacgag ataggttttc ataaatcaaa gttctccatt ccagtttgca aaaaagtcct
      421 tgagggcatc ctgccagtcg cgcatctcgt cgccgacggt gcagcgcagc atgcggttct
      481 ccagggcgct ccacgccggg cggttggcac tggcggggtt ggcggcggcg tactgggcgc
      541 tggtgacagg ctcgaccttg caggaaagcc ctgccccctg catgatggcg gacgcgaaat
      601 cataccagct gcaaatgccg ttgccggtgc agtggtagat gccgtaatcg tggctgacgg
      661 ccagcttgag aatatggtag gccaaatcga cggcgttcgt cgggttgccc agctggtcgt
      721 tgacaacggt gattttgtcg tgggtccggc ccaggcgaat catcgtcttt acgaagtttt
      781 tgccgtagta gctgtacagc catgccgtgc gcacaatgaa gtggcggcgg cagaaccgct
      841 cgacatactg ctcacccagc agcttggtct ggccgtaggc agagatgggc gcgggcacgg
      901 cgcactcgtc cagtgcaacg ccgccgttgg ccgtgccggg gaacacatag tcggtggaaa
      961 catggataag ccgcgccccg attttctcgc acgccagggc caggttgcgc gggccgatgg
     1021 cgttgacttt aaacgccgcg tcgcgggcgg tctcgcagcc gtccacgttg gtgaaggcgg
     1081 cacagttgat gaccgtatcg ggctggtggc ggcggatgta gttgatggtg gcctcccggt
     1141 cggtgatgtc cagttcgtcc acatctaccg ggatgaccgt ggctttgcgc aggcgctcgg
     1201 gcagcgggcc gatggcgcag ccttcctcag aaagctggcg ctgcagctcg gtgcccagct
     1261 gcccgcggca gccggtaatc aaaattttca tgcgtactcc cctgccctat cagtattgca
     1321 gcttgtcgtc ggcgacattc ttcaaatgct ggccgtaggg ggatttgcca tagcgggcgg
     1381 cgctttccag cagcttttcc ttggtgatcc agccgtactt gtaggcaatt tcttccgggg
     1441 cagagatttt gatgccctgg cgctgctcaa cagtctgcac aaaggtagca gcctcgacga
     1501 gggagtccat cgtgccggtg tcgagccagg catagccgcg gcccagcagc tggacatcca
     1561 gcttgccctg gtcgaggtac atctggttca gtgtggtgat ttccagctca ccgcgggcac
     1621 tgggcttgac ctgctttgcc atctttacga cctcattatt gtagaagtag aggccggtga
     1681 cggcgtagtt gctcttgggg ttagcgggtt tttcctcaat gtcggtggcg tggccgtcct
     1741 tgtcaaacgc gacgacgccg aagcgttcgg ggtcgttgac atagtagccg aacaccgtgc
     1801 tgcggccgtt ggcggcgttg gcggcggcgg tcttcaggcg cttggagaaa ccggcgccgt
     1861 agaagatgtt gtcgcccaga accatggcgc agcagtcgtc accgatgaac tcctcaccca
     1921 ggatgaacgc ctgggccagg ccgtccggag aaggctggac cttatactgc agatgcaggc
     1981 cgtactggct gccgtcgccc agcagcgcct caaagcgcgg ggtgtccgtg ggggtggaaa
     2041 tgatcagaat gtcctggatg cccgccagca tcagcgtaga cagcggatag tagatcatcg
     2101 gcttgtcata gacgggcagc agctgcttgg atgtgaccat cgtcagcggg taaagtcggg
     2161 tgccggcgcc gccggcaagg ataatacctt tcatcgttac aactccttta aaattttggt
     2221 gcagtgcgcg ccgcccatcc cattggggca taatctcaca caatacgatt aaagttatta
     2281 tacagcattt tgcaagggca cgcaaggggg aaatcttgcc gtgatgtgta cccatttgtt
     2341 gccaatttgt tactatttct ttgcaatgcc ttgacggata gaacaagctg tgctacaata
     2401 caaacagtat aaaccacgat ggcacgtcgc tgcatcgttt ttgatagaag ctaaagactg
     2461 aatatgagga ggcgttttct tcgatgaaac gctttttttg tgcggccttt tttgtggccg
     2521 tcgttgcgtt gctggccgcc tgcggcgcac tgaatgtaaa ctccgccgca gcccaggcta
     2581 cggctacccc caccagctct acttcccgca cacagaagag tgattccacc gttaaggtca
     2641 acgagaccgc cgccaaggtg gatgtcgtca cggcgcagag cagcgccagc cccaccgcta
     2701 cggcggcccc ggcccaggtc gagaccccgg ccaccgggga ttttcctgtt gtgtatctgg
     2761 ctggcgtggt aggtcttatc ctgatttgct ccgccgggct gctgatgctg cgcaaaatct
     2821 gatgtaaacg ccctccgggg cgttttttgt ttttaagcct cccctgtcaa aaaagggtag
     2881 acgccgaagg cggccagaga gatcccgacg agcgttccct tatgtgccgc cactttcaca
     2941 gaaaggagcc ccgtacatgc ccagacgcta ccgcaagctg cgcggggata ccgcgtacta
     3001 ccctttcgcg gcccgcaccg ggttacgcgc ccggcagcgc cgccatgcgc ggctgctgcg
     3061 cgttctgggc acagcggcgc tggccctggc ggcggtgctg ctggtgcggc agactgtgtt
     3121 ccgtggcggc cagcccaccg cccccgcgcc gattgccgcc gcacagacca ccgcccccgg
     3181 gcaggcccat gtgaccgttg cgccggttgc cgcgacccgg cactttctga ttaacggcat
     3241 tgctgcccct gaaaaac
//
LOCUS       DBMDSQ010000232         3249 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_6665_length_3249_cov_1.527001, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000232 DBMDSQ010000000
VERSION     DBMDSQ010000232.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 3249)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3249)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3249
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6665_length_3249_cov_1.527001"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..830
                     /locus_tag="ACZPAX_08140"
     CDS             <1..830
                     /locus_tag="ACZPAX_08140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113904.1"
                     /translation="FALNNVALSDTYATQYKEFLNFESPIQYSTDKNSWTTELSGFKF
                     VDAGSYTLYAKQEVIVTLTDFEGKTKTEPRDVVGQATYTITPRDVTLTSGSAEKEYDR
                     TALTNNTVTAEGFVNGEGATYTVTGTQTEVGSSENKFNYTLNSNTKAGNYNITTQCGT
                     LTVTEKTTPDPVDPKTNPDTTPTPTPTPIVTPAPTAAPSTDDNAPENTATPAPTATAA
                     PTATPAPAPTAAPAAARAATTIPQTGDNFPAVALVIVMLVAIVGLGITVVLRTDKKK"
     gene            1050..2321
                     /locus_tag="ACZPAX_08145"
     CDS             1050..2321
                     /locus_tag="ACZPAX_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005921083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HIR1113905.1"
                     /translation="MDDGSTFMIVALIVLIGLSAFFSAAETAYNSLNEIRLKSKADDG
                     DEKAAHTLALVERYDSLLSTILIGNNIVNIGASSLATVLFTRLVGGVYGPTVSTIVMT
                     LLVLTFGEITPKSMAKEMPETLAMAFAPVLSALVTIFTPLNALFGAWKKFLAKRFDTG
                     EKDTITEGELVTMVSEAEKDGELTNRESELIRSAIEFDDVEAQDVLTPRVDVVAVEDD
                     TPMDEVIERFAESGYSRLPVYHDTIDNIIGVVHEKDCFAALQKHDKNVKLENLIGPTL
                     YTTSVTQISALLRTLRESKHHMAVVVDEYGGTAGIITLEDILEELVGEIWDEHDDVVE
                     DVRQQSDGSWLVSGSASVDDTAEELGIKEDDDIDAVAIGGLVQEKLSRLPKVGDRFIW
                     GDFDGTVTRATNRRVQEVRLTPRKVQDENEK"
     gene            2652..>3249
                     /locus_tag="ACZPAX_08150"
     CDS             2652..>3249
                     /locus_tag="ACZPAX_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015567205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase-like
                     domain-containing protein"
                     /protein_id="HIR1113906.1"
                     /translation="MAEQNLGPRDFFAKEDAIADLGLIACPGAEELCKLVDSHLVRWA
                     REVGNTDVDTFIIPSDCPRFQSGDAKGLVKASTRGDDLYIFVDPGNYSVTYQLFGYEN
                     HLSPDDHFQNLMRLIQAVAGRAHRISVIMPSLYGGRQHRRVSRESLDCAFALQQLRDV
                     GVKNIITFDAHDPRVMNAVPTMSFDNVMPTYQVLKCLLH"
BASE COUNT          764 a          954 c          879 g          652 t
ORIGIN      
        1 ccttcgcact aaataatgtg gcattgtcag atacatacgc gacgcagtat aaagaatttt
       61 tgaatttcga gagtccgatt cagtactcaa ctgataaaaa ttcttggaca acagaattaa
      121 gtgggtttaa gtttgtcgat gctggtagtt atacactcta tgccaagcag gaagttattg
      181 ttaccctgac tgattttgaa gggaagacca agacggaacc gcgggatgtc gtcggccagg
      241 ctacctacac catcaccccg cgcgatgtta cgctgaccag cggcagcgcc gagaaagaat
      301 acgacagaac tgcgctgacg aacaacaccg ttacggcgga aggtttcgtg aacggtgagg
      361 gtgcgaccta caccgtcacc ggcacgcaga ccgaagttgg ttcttccgag aataaattca
      421 actacacgct gaacagcaac acgaaggcag gcaactacaa cattaccacc caatgcggca
      481 ccctgacggt cacggaaaaa accacgcctg acccggttga ccccaagacg aaccccgata
      541 ccacgccgac cccgacaccg acgcccattg tgacgcccgc gccgacggca gcgccgagca
      601 ctgacgacaa cgcgccggag aacaccgcaa ccccggcccc gacggcgacc gccgcaccca
      661 cggccacgcc cgcccctgcg ccgactgccg ccccggcagc ggcccgggct gcaacgacca
      721 tcccgcagac cggcgacaat ttcccggctg tggcgctggt cattgtgatg ctggtcgcga
      781 ttgttggcct tggcatcacg gttgtccttc gcacagataa gaaaaagtaa atctgcatag
      841 gcagtgccgc cccggcccgg ttgggtcggg gcgatttttg ttccttcttt gcaaagaagg
      901 aaccgaagaa actccaaata tagataaata gacgttttta cccaaatttg acggtatacc
      961 ctttgcgtat cccgcatatc tatgatataa tagctaaaat gtgtattttt gcacataaat
     1021 aaagaataga aagagttgat agttccccca tggacgatgg cagtacattt atgatcgtcg
     1081 cactgatagt tctgattggt ttatccgcgt ttttctcagc ggccgaaaca gcgtacaatt
     1141 cgctgaacga gattcgtctg aaaagcaaag cggatgatgg tgacgagaaa gccgcccata
     1201 cgttggccct ggtcgagcgc tatgacagcc tgctcagcac cattctgatt ggaaacaata
     1261 tcgtgaacat cggtgcgtcc tcgcttgcca ccgtgctgtt tacccgcttg gtgggcggtg
     1321 tatacggccc cacggtctcc acgatcgtca tgaccctgct ggtcctgacc ttcggcgaga
     1381 tcacccccaa gagcatggca aaagaaatgc ccgaaaccct ggccatggcc tttgccccgg
     1441 tgctcagtgc gttggtcacg atttttacgc cgctgaacgc cctgttcggt gcgtggaaaa
     1501 agttcctcgc caagcggttt gacactggcg agaaagacac catcaccgaa ggcgagctgg
     1561 tcacgatggt cagcgaagcc gagaaggacg gcgagctgac caatcgcgaa agcgagctga
     1621 tccgcagtgc catcgagttt gacgacgtcg aggcccagga tgtgctgacc ccccgcgtgg
     1681 atgtggtcgc cgtcgaggac gataccccga tggacgaggt catcgagcgc tttgccgaga
     1741 gcggctattc ccgtctgccg gtctaccatg acacgatcga taacatcatc ggcgtggtgc
     1801 atgagaagga ctgctttgcg gctctgcaaa aacacgacaa gaacgtcaag ctggagaacc
     1861 tgattggccc gacgctctac accaccagcg tgacccagat ttccgcgctg ctgcgcaccc
     1921 tgcgcgagag caagcaccac atggccgtcg tcgtcgatga gtacggcggc acggcgggca
     1981 tcatcacgct ggaggatatt ctggaggagc tggtcggcga gatttgggac gaacatgacg
     2041 acgtggtcga ggatgtccgc cagcagtccg atggcagctg gcttgtctcc ggcagcgcca
     2101 gcgtggacga caccgccgag gaactgggca tcaaagagga tgatgatatc gacgctgttg
     2161 ccatcggcgg cctggttcag gaaaagctca gccgcctgcc taaggtgggg gacaggttca
     2221 tctggggcga ctttgacggc accgtcaccc gcgccaccaa ccgccgcgtc caggaagtcc
     2281 gcctgacccc ccgcaaagtc caggacgaga acgagaagta aatagaatga aatctcctgc
     2341 tggattccag caggagcctt tttgtaggag cagagccccg ctcctacggc gccagctgac
     2401 cgataaaatg ttactgtaaa tgtttgtgaa aattcccttt gcaaattttt tgtaaaatcc
     2461 gacagaattt ttcgttggcc gcggccaaca caatgtctaa agtgcggaaa atgtagaaat
     2521 tgctgaaatt cttgccttgc agggctgatt ttatcccgcc gctgtgctac aatactagta
     2581 aatggggcat tgcggcacgc cgcgccgccc cgccgcaaat gaatacaaga tggaagtaag
     2641 gagtacctgt aatggctgaa cagaatcttg gcccgcgtga tttctttgcc aaggaggatg
     2701 cgatcgctga tctcggcctg atcgcctgcc ccggtgcaga ggaactttgc aaactcgttg
     2761 atagtcacct tgtccgctgg gcgcgtgaag tcggcaacac cgatgttgac accttcatca
     2821 tcccgtccga ctgcccgcgt ttccagtccg gcgacgccaa gggcctggtc aaggcctcga
     2881 cccgcggcga cgacctgtat atcttcgtcg atccgggcaa ttacagcgtt acctaccagc
     2941 tgttcggcta tgagaaccac ctctccccgg acgaccactt ccagaacctg atgcgcctga
     3001 tccaggctgt tgcaggccgc gcccaccgca tcagcgttat catgcccagc ctgtacggtg
     3061 gccgtcagca tcgccgcgtc agccgcgaaa gcctggactg cgcttttgcg ctgcagcagc
     3121 tgcgcgacgt cggcgtcaag aacatcatca cctttgacgc ccatgacccc cgcgtcatga
     3181 acgccgtgcc caccatgagc ttcgacaacg tcatgcccac ctaccaggtg ctcaagtgcc
     3241 tgctgcatc
//
LOCUS       DBMDSQ010000233         3197 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_6796_length_3197_cov_1.998385, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000233 DBMDSQ010000000
VERSION     DBMDSQ010000233.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 3197)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3197)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3197
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6796_length_3197_cov_1.998385"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..899
                     /locus_tag="ACZPAX_08155"
     CDS             <1..899
                     /locus_tag="ACZPAX_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HIR1113907.1"
                     /translation="DFICGKGGVIDTWLNLGASGFRLDVADELPDDFIEKIRTAVKSH
                     GDDKLLLGEVWEDATTKEAFGQRRTYLRGRGLDAVMNYPFRNAALDYLHGGDVAAVAD
                     ALMQICENYPAPALDCAMNFLSTHDTERAITAIAGEPCNGRDRCWQSKRCIPADKMDD
                     AVRKLLLGYAMIFTLPGVPCVYYGDEIAMQGYRDPFNRAFFDWNSTEQRLRGPIKTLA
                     ALRRSCDAFDGGQLEIVRAEGDVLHYRRVGKTQTAEIILNRGPHLLAEEAFGKNTEVN
                     PGGFTVLVEDNEPEHVGYFSVY"
     gene            1112..2251
                     /locus_tag="ACZPAX_08160"
     CDS             1112..2251
                     /locus_tag="ACZPAX_08160"
                     /EC_number="7.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015538182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HIR1113908.1"
                     /translation="MQFLQTFAGLFANLGNLTWQMVVMWVIGGLLIYLAIAKKMEPSL
                     LLPMGFGAILVNLPLSGAITQGETVGPINALFDAGMSNELFPLLLFIGIGAMIDFGPL
                     LEKPWLMLFGAAAQLGIFVTLVLAGLFFDLPDAASIAVIGAADGPTAIFVANTLGSKY
                     LGAIMVAAYSYMALVPIVQPPVIRLCTTKKERLIRMPYQKGNVSKITKILFPIVVTML
                     AGLVAPASVALVGFLMFGNLLRECGVLNALSETAQNVLANLITIVLGLTVAGQMTADK
                     FVQPDTLLILALGLVAFIFDTAGGVFFAKLLNLFLPEGKKINPMIGAAGISAFPMSGR
                     VVNQMGLAEDNQNFLLMYSISVNVSGQIASVIAGGLILTLMSSCV"
     gene            2264..2389
                     /locus_tag="ACZPAX_08165"
     CDS             2264..2389
                     /locus_tag="ACZPAX_08165"
                     /inference="COORDINATES: protein motif:HMM:NF040909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OadG-related small transporter subunit"
                     /protein_id="HIR1113909.1"
                     /translation="MSTALLPVTLSMLCAGMAGIFLVLGVLVLGVYALNKFFSGK"
     gene            complement(2459..>3197)
                     /locus_tag="ACZPAX_08170"
     CDS             complement(2459..>3197)
                     /locus_tag="ACZPAX_08170"
                     /inference="COORDINATES: protein motif:HMM:NF014679.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HIR1113910.1"
                     /translation="QLDDGTMTKDEFFVRLAEDSKLPRTSQPSPASFMQVYEDAAAAG
                     DEVLVITIGQKLSGTYQCAHLAAADVGLQAHIVDSEAASQAEALLVREAVRLRDEEGL
                     TAEEIAAVLEQFKKRVRIVAVVDSLKHLQKGGRLPAAVALVGGALGIKPVLALQDGAI
                     KLVDKGRGRPGALVAMFKQLDALGGVDPRYGYTLLYSDNKQLIAPVHHYMHQNLQLTG
                     GRVAQLGPTIGTHVGPGVVGVVFVAKE"
BASE COUNT          578 a         1015 c          949 g          655 t
ORIGIN      
        1 tagattttat ctgcggcaag ggcggcgtca tcgacacttg gctgaacctg ggcgcgtcgg
       61 gcttccggct ggatgtggcc gacgaactgc ccgatgattt cattgaaaaa atccgcactg
      121 ccgtcaaatc ccacggcgac gacaagctgc ttctaggcga agtgtgggag gacgcgacca
      181 cgaaggaagc cttcggccag cgccgtacct acctgcgcgg gcgcgggctg gacgccgtga
      241 tgaactatcc cttccgcaat gcggcgctgg actacctgca cggcggcgac gtggccgctg
      301 tggccgatgc cctgatgcaa atttgcgaga actatcctgc cccggcgctg gactgtgcca
      361 tgaatttcct gtccacccac gacaccgagc gcgccatcac cgccattgcg ggcgagccct
      421 gcaacggccg cgaccgctgc tggcagagca agcgctgcat ccctgccgac aaaatggacg
      481 atgctgtgcg caaactgctg ttggggtatg cgatgatctt cacgctgccc ggcgtaccct
      541 gcgtctacta cggcgacgag atcgccatgc agggctaccg cgaccccttc aaccgggcgt
      601 tctttgactg gaactccacc gagcagcggc tgcgcggccc catcaagacg ctggccgccc
      661 tgcgccgcag ctgcgacgcc ttcgacggcg ggcagctgga aatcgtccgc gctgaggggg
      721 atgtgctgca ctaccgccgc gtcggcaaaa cccagacggc agagatcatc ctgaaccgcg
      781 ggccgcacct cctcgccgag gaggccttcg gcaagaacac cgaggtcaac cccggcggct
      841 tcacggtgct ggtcgaggac aacgagcccg agcatgtggg gtatttttcg gtatattgag
      901 caaatttccc ttgcattttt cgcgccccta tgctataatc cactcaaggt aaaagggagt
      961 aagggacgcg ccgccgtgcg tcgtatcgct tcgtcaccac gggctttggc cccggggcat
     1021 actgaaacac tgagactttt gccgcagact ttttctgcgg caggggtctc tttttttgct
     1081 ccctgccgcc cacgattcgg gagggtcctg tatgcaattt ctccaaacct ttgccgggct
     1141 gttcgcaaac ctcggcaacc tcacctggca gatggtggtc atgtgggtca tcggtggcct
     1201 gctgatctac cttgccatcg ccaaaaagat ggagccgagc ctgctgctgc ccatgggctt
     1261 cggtgccatc ctcgtcaact tgccgctgtc cggtgccatc acacagggcg aaaccgttgg
     1321 ccctatcaac gcgctttttg acgccggtat gtccaacgag ctgttcccgc tgctgctctt
     1381 tatcggcatc ggcgcgatga tcgactttgg cccgctgctg gaaaagccgt ggctcatgct
     1441 gttcggtgcg gcggcgcagc tgggcatctt tgtcacgctg gtgctggcgg ggctgttctt
     1501 tgacctgccc gacgcggcgt ccatcgcggt catcggcgcg gcggacggcc cgacggctat
     1561 cttcgtggcg aacacactgg gcagcaaata cctgggtgcc atcatggtcg cggcgtacag
     1621 ctacatggcg cttgtgccca tcgtgcagcc gccggtcatc cgcctgtgca caacgaaaaa
     1681 agagcggctg attcggatgc cgtaccagaa aggcaatgtc agcaaaatca ccaaaatttt
     1741 gttcccgatt gtggtcacta tgttggccgg gttggtagcc cccgccagtg tggcgctggt
     1801 cggcttcctc atgttcggca acctgctgcg cgagtgcggc gtgctgaacg ccctgtctga
     1861 gacggcacaa aacgtgctgg caaatcttat cacgatcgtg ctgggcctga ccgtagccgg
     1921 gcagatgacc gcagacaagt ttgtgcagcc cgacacgctg ctgatcctgg cgctgggtct
     1981 ggtcgcgttt atttttgaca ccgccggcgg cgttttcttt gccaagctgc tgaatttgtt
     2041 cctgcccgag ggcaaaaaaa tcaacccgat gatcggtgcg gcaggcattt ccgcgttccc
     2101 gatgagcggg cgcgtcgtca accagatggg tctggctgag gacaaccaga atttcctgct
     2161 gatgtactca atcagtgtca acgtctcggg ccagatcgcg tcggtcattg ccggcggcct
     2221 gatcctgacc cttatgagca gctgcgtata aggaggtcaa cccatgtcaa ccgcacttct
     2281 tcccgtcacc ctctcgatgc tctgcgccgg tatggccggc atcttcctcg tcctcggcgt
     2341 cctcgtgctg ggggtgtatg cactgaataa gtttttcagc ggaaaataaa aaagcttctc
     2401 cgaatggaga agctcaactt acaggggcgg gcattgctcg cccctgcatt ttattttatt
     2461 actccttcgc cacaaacacc acgccaacca cgccggggcc gacatgggtg ccgatggtgg
     2521 ggccaagctg ggccacacgg ccgccggtca gttggagatt ctggtgcatg tagtggtgta
     2581 ccggtgcaat aagctgcttg ttatcgctgt agagcagcgt atagccgtag cgtgggtcaa
     2641 caccgccgag cgcgtccagc tgcttgaaca tggcgaccag cgcaccgggg cggccgcggc
     2701 ccttgtccac caacttgatg gcaccgtcct gcagggccag caccggcttg atgcccagcg
     2761 caccgcccac cagcgccacc gctgccggca gccggccgcc cttctgcaga tgcttgaggc
     2821 tgtccaccac ggcaacgatg cgcacccgct tcttgaactg ttccagcaca gcggcgatct
     2881 cctccgccgt caggccctcc tcatcacgca ggcgcaccgc ctcacggacg agcagcgcct
     2941 cagcctgcga agccgcttcg gagtctacga tatgcgcctg caggccgaca tcggcggcgg
     3001 ccagatgcgc gcactggtag gtgccggaca gcttctggcc gatggtgatc acaagaacct
     3061 catcccccgc cgcggcggcg tcctcataca cctgcataaa gctggccgga ctgggctggc
     3121 ttgtgcgggg cagcttgctg tcctctgcca gacggacgaa aaattcatcc ttcgtcatcg
     3181 tgccgtcatc aagctgg
//
LOCUS       DBMDSQ010000234         3195 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_6806_length_3195_cov_1.562379, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000234 DBMDSQ010000000
VERSION     DBMDSQ010000234.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 3195)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3195)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3195
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6806_length_3195_cov_1.562379"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..550)
                     /locus_tag="ACZPAX_08175"
     CDS             complement(<1..550)
                     /locus_tag="ACZPAX_08175"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR1113911.1"
                     /translation="MYKNIIFDFGGVVVDFAPKDFLMDHFMNRRAEEETYELVFGSQE
                     WQDLDRGTITREAANKQMLEHAAEAGRIFEVQTCIDEWATMLRTKKTTVQIMRKLKAA
                     GYRLYYLTNIPTDIMDELRQREWFSLFDGGIASCDVHLCKPEPEIFTTLMQTCRLAYD
                     ESIFVDDNKANAQAAYNLGITGI"
     gene            634..888
                     /locus_tag="ACZPAX_08180"
     CDS             634..888
                     /locus_tag="ACZPAX_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HIR1113912.1"
                     /translation="MTLRDLSNKDVIQLKTGENLGRIDDIVFDERGARLQSVILRGRA
                     HCFGLLGCDDDLILPWESIKTIGTDAIMVDAEPLPGTRRR"
     gene            992..1345
                     /locus_tag="ACZPAX_08185"
     CDS             992..1345
                     /locus_tag="ACZPAX_08185"
                     /inference="COORDINATES: protein motif:HMM:NF015867.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HIR1113913.1"
                     /translation="MQTTRKLAALTAAAAVLIILGSLFLIAPVWQPQSPAAVYIPAAV
                     AAPTPTPAPAIININTATAEELTTLPGLGPEKAAALVAYRTAHGAFASLADAANVKGI
                     SLRMTESWVGLAAVQ"
     gene            1361..>3195
                     /gene="ppk1"
                     /locus_tag="ACZPAX_08190"
     CDS             1361..>3195
                     /gene="ppk1"
                     /locus_tag="ACZPAX_08190"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HIR1113914.1"
                     /translation="MEKKPESIFINRELSWLDFDSRVLALAKEKTVPLGERIKFAAIF
                     GSNMDEFFMVRVGSLYDQTLLKNNKTDNVTHMTAAEQIAAITPRVAELQAKCDKYFQH
                     LVTALATEGYRKVDFAKLSKPQEHFWKTYFQRELFPLLSPQIVDSRHPFPFLNNKDIY
                     YIAQLHTKNEGVSYGIVPVSSQFERVLFVKDGETACFAFVEELIAHYAATIFSASTVT
                     KQCLFRVTRNADITVDEGMMDHDVDFRDVMSELLKKRRKLAAVRLQFWPDAPQEIVKF
                     LRDKLVVPADRCYTQTSPLDSGALFKLAGRISGDGGHTELFYPTARPMQAPAGYDLYT
                     EVRKHDVLLAYPYQSIRPFIKMLLRAGADPDVVSIKMTLYRMASDSQIVNALIAAAEN
                     GKEVVAMVELRARFDEQNNIDWSKQLEDAGCTVFYGFDDYKVHSKLTLITSRVNGQYK
                     YLTQIGTGNYNEKTSELYTDLSFITTRQEIGEEASAVFNNMALQRLTSEADTMLVAPL
                     RFKSVLLEEMDRQIALAMQGKPASIILKNNSINDPQIIDKISEASCAGVRVDMIVRGI
                     CCVRAGVPGRTENVHIRSLVGRYLEHSRIYCFGSGENMRIYIASG"
BASE COUNT          715 a          971 c          876 g          633 t
ORIGIN      
        1 aaataccggt gatgcccagg ttgtacgcgg cctgggcatt tgccttgttg tcgtccacaa
       61 aaatcgactc atcgtaggcc aggcgacagg tctgcatcag cgttgtaaaa atctccggct
      121 cgggcttgca caggtgtacg tcgcaggacg caatcccgcc gtcgaacagt gagaaccact
      181 cccgctggcg cagctcgtcc atgatgtcgg tgggaatgtt cgtcaggtag tacagccggt
      241 agcctgcggc tttcagcttg cgcatgatct gcacggtcgt cttttttgtg cgcagcatcg
      301 tggcccactc gtcaatgcag gtctgcacct caaatatgcg gcctgcctcg gcggcgtgct
      361 ctaacatctg cttgttggcg gcctcgcggg tgatggtgcc ccggtccaga tcctgccact
      421 cctggctgcc gaaaaccagc tcgtaggttt cctcctcagc gcggcggttc atgaaatggt
      481 ccatcaagaa atccttgggg gcaaaatcca ccacaacgcc gccaaaatcg aaaataatat
      541 ttttatacat aaaaacctcc gcaaggtgca tagcatattt tctactatta tacacgcaat
      601 ttgcatagga tacaagagaa agggggctat cccatgacat tgcgtgattt gagcaacaag
      661 gatgtcatcc agctgaaaac cggcgaaaac cttggccgca tcgacgatat cgtttttgac
      721 gagcgggggg cgcggctgca atcggtcatc ctgcgcgggc gggcgcactg ctttggcctg
      781 ctgggctgcg atgatgacct gatcctcccg tgggagagca tcaagaccat cggcaccgat
      841 gctattatgg tggacgccga gccgctgccc ggtacccgcc gccgctaagg caagaaaatc
      901 gtatattttg tgtaaaattc cgcaaaagca gatgcttttt ttagagcaat gtgctataat
      961 taaaattcaa caggaaatgg gggacacggc catgcagaca acccggaaac tcgcagcgct
     1021 gacggctgcc gcagcggtgt tgattatcct ggggtcgctg ttcctcatcg cgcctgtgtg
     1081 gcagccgcaa agccccgccg ctgtgtatat cccggcggct gtggcagcgc cgacgccgac
     1141 tcccgccccg gcaatcatca acatcaacac cgctactgcc gaggaactga ccaccctgcc
     1201 gggccttggc cccgagaaag ccgccgccct tgtcgcatac cgcaccgccc acggcgcatt
     1261 tgcgtcgctg gctgacgcgg ccaacgtcaa ggggatatcc ctgcgcatga cagaaagctg
     1321 ggtgggtctt gccgccgtac aataaatgga ggagatcaac ttggaaaaaa agccggaaag
     1381 catcttcatc aaccgtgaac tcagctggct cgactttgac agccgcgtac tggccctagc
     1441 aaaggaaaag accgtgcctt tgggcgagcg catcaagttt gccgccatct ttggcagcaa
     1501 catggacgag ttctttatgg tgcgcgtcgg ttcgctctac gaccaaacgc tcttgaaaaa
     1561 caacaagacc gacaacgtca cccacatgac cgccgccgag cagattgccg ccatcacgcc
     1621 ccgcgtggcg gaattgcagg ccaagtgcga taaatatttc cagcaccttg tcacggcact
     1681 ggccaccgag ggctacagga aagtcgattt cgccaagctg tccaagccgc aggaacattt
     1741 ctggaaaacc tacttccagc gcgaactctt cccgctgctc agcccccaaa ttgtcgactc
     1801 ccgccatccg ttcccgtttt tgaacaacaa ggatatctac tacatcgccc agctgcacac
     1861 gaaaaacgag ggcgtcagct acggcatcgt gccggtcagc agccagtttg agcgcgtact
     1921 cttcgtcaag gacggcgaga cggcctgctt cgcctttgtg gaagagctca tcgcccatta
     1981 cgccgccacg attttcagcg ccagcaccgt gaccaagcag tgccttttcc gcgtgacccg
     2041 caatgccgat atcacggtcg acgaaggcat gatggaccac gacgttgact tccgcgacgt
     2101 catgagcgag ttgctgaaaa agcgccgcaa gctcgctgcc gtgcgcctgc aattctggcc
     2161 cgacgccccg caggaaatcg tcaagttcct gcgggacaag ctcgtcgtcc cggcggaccg
     2221 ctgctatacc cagacttcgc cgctcgactc cggcgcactg ttcaagctgg ccgggcgtat
     2281 ctccggcgac ggcggccaca cggagctgtt ctatcccacc gccaggccga tgcaggcccc
     2341 ggcagggtac gacctctaca ccgaggtgcg caagcatgat gtgctgctgg cttacccgta
     2401 ccagagtatc cgcccgttta ttaaaatgct gctgcgcgcg ggcgcggatc cggacgttgt
     2461 atcgataaaa atgacgctct accgcatggc aagcgactcc cagatcgtca acgccctgat
     2521 tgccgccgcc gagaacggca aggaagtcgt cgccatggtc gagctgcgcg cccgctttga
     2581 cgagcagaac aacatcgact ggtcgaagca gctcgaggat gccggctgca cggttttcta
     2641 cggtttcgat gactataaag tacattccaa attgaccctt atcaccagcc gcgtcaacgg
     2701 ccaatataaa tacttgacgc agatcggtac cggcaactat aatgaaaaga cgagcgagct
     2761 gtacaccgac ctgtccttca tcacgacccg gcaggagatc ggcgaggaag ccagcgccgt
     2821 gttcaacaat atggccctgc agcgcctgac cagtgaggca gacacgatgc tcgtcgcgcc
     2881 gctgcgattc aagagcgtat tgctcgagga aatggaccgc cagatcgccc ttgccatgca
     2941 gggaaagccc gcgtcgatta tcctcaaaaa caactccatc aacgacccgc agattattga
     3001 taagatcagc gaggccagct gcgcgggcgt gcgcgtggat atgatcgtgc gcggcatctg
     3061 ctgcgtacgg gcaggcgtgc cgggccgcac cgagaacgtg catatccgca gcctggtcgg
     3121 gcggtatttg gagcattccc gcatctactg cttcggcagc ggcgagaata tgcgcattta
     3181 tatcgcctcc ggcga
//
LOCUS       DBMDSQ010000235         3089 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_7080_length_3089_cov_2.002677, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000235 DBMDSQ010000000
VERSION     DBMDSQ010000235.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 3089)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3089)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3089
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7080_length_3089_cov_2.002677"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            111..>409
                     /gene="pgk"
                     /locus_tag="ACZPAX_08195"
     CDS             111..>409
                     /gene="pgk"
                     /locus_tag="ACZPAX_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004063754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HIR1113915.1"
                     /translation="MGLGKKTIDDENYAGKRVLVRCDFNVPMKDGVITNDNRINAALP
                     TIKKLINDGAKVILCSHLGKPKNGPEAKFSLAPVAVRLSEKLGQPVIFADDDTVVG"
     assembly_gap    410..419
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <420..1299
                     /locus_tag="ACZPAX_08200"
     CDS             <420..1299
                     /locus_tag="ACZPAX_08200"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015538191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HIR1113916.1"
                     /translation="GNGDVVLLQNTRFRKEETKNEPAFSEELASLADAYVDDAFGSCH
                     RAHCSTAGVTDYIKDTAVGYLMEKEIKYLGNAVNDPERPFTAILGGAKVADKLNVISN
                     LLEKVDTLIIGGGMAYTFLKAQGYEIGKSLCDDSKLDYCKEMMAKAQEKGVKLLLPVD
                     AACIKDFPDPIDAPIETTIVPVSAIPADQEGCDIGPETMKLFADAVKASKTVVWNGPM
                     GVFENPTLAAGTLAVAKAMAESDATTVIGGGDSAAAVQQMGLGDKMTHISTGGGASLE
                     YLEGKELPGIAVIQKA"
     gene            1400..2170
                     /gene="tpiA"
                     /locus_tag="ACZPAX_08205"
     CDS             1400..2170
                     /gene="tpiA"
                     /locus_tag="ACZPAX_08205"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005945612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HIR1113917.1"
                     /translation="MDKQNRKAIIAGNWKMNKTATEAKALISELIPAVKDAGCEVVIC
                     VPFTDLVTAVEMTKGTNIHVGAENVHFEKSGAFTGEISADMLTDLGVEYVVIGHSERR
                     QYFAETNETVNKRTKAALAGGLKPIICVGESLDQREQGVTEELVRMQVKIALNGVTAD
                     ELKNVVIAYEPIWAIGTGKTATADQAEEVCAAIRKVVGELYGEDAAKALTVQYGGSMN
                     PKNAEELLNKPDVDGGLIGGASLKADQFAVIVDAATKG"
     gene            2249..>3089
                     /gene="gpmI"
                     /locus_tag="ACZPAX_08210"
     CDS             2249..>3089
                     /gene="gpmI"
                     /locus_tag="ACZPAX_08210"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005922312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HIR1113918.1"
                     /translation="MSKKPVMLCIMDGFGWTPDKTFGNAVAAANKPNLDKLFANYPMT
                     TIEASGMAVGLPDGQMGNSEVGHTNMGAGRIVYQQLTLITKSIKDGEMLKNPVLVKNM
                     KAAIDAGKAIHLMGLVGTGGVHSHADHWYGVLEMAKHMGAKDVYLHCIMDGRDTDPHS
                     GKGFLADLQAKLDELGIGKIASVSGRYYAMDRDNNWDREEKAYAAFVYGEGNHAANAQ
                     EAIEASYAADVTDEFVIPVVTCEGGRVEDGDTVIFMNFRPDRARQMTRIFCDDAFTGF
                     ERRG"
BASE COUNT          688 a          950 c          884 g          557 t
ORIGIN      
        1 ttaaaaatat atcaaaaaca cttgcgcaaa ttccttttta tgcgtataat atgggtattg
       61 ggcgcaaaac gtccccataa caatgtgtaa taccgagagg agcttttact atgggtttgg
      121 gtaaaaagac cattgacgat gaaaactacg ctggcaagcg cgtgctggtt cgctgcgact
      181 tcaacgtccc gatgaaggac ggcgtgatca ccaatgacaa ccgtatcaac gcggctctgc
      241 cgaccatcaa aaagctgatc aatgatggcg ccaaggtcat tctgtgcagc cacctgggca
      301 agccgaagaa cggccccgag gccaagttca gcctggcccc tgttgctgtc cgcctgagcg
      361 agaagctggg ccagcccgtc atttttgctg acgatgacac cgttgtcggn nnnnnnnnng
      421 ggcaatggcg atgttgttct gctgcagaat acccgtttcc gcaaggaaga gaccaagaat
      481 gagcccgctt tcagcgagga gctggccagc ctggccgatg cttacgtgga tgacgctttc
      541 ggctcctgcc atcgcgccca ctgctccacc gccggtgtta ccgactacat taaggatacc
      601 gctgtcggtt acctgatgga aaaggagatc aagtacctgg gcaacgcagt gaacgatccc
      661 gagcgtccct tcaccgccat tctgggcggc gctaaggtcg ctgataagct gaacgttatc
      721 tccaacctgc tggaaaaggt cgacaccctg atcatcggcg gcggcatggc ctacaccttc
      781 ctgaaggccc agggctacga gatcggcaag agcctgtgcg acgactccaa gctggactac
      841 tgcaaggaaa tgatggccaa ggcccaggag aagggcgtca agctgctgct gcctgttgac
      901 gctgcctgca tcaaggactt ccccgatccc atcgatgctc ccatcgagac caccattgtt
      961 ccggtcagcg ccatccctgc tgaccaggaa ggctgcgaca tcggccccga gaccatgaag
     1021 ctgtttgctg atgccgtcaa ggccagcaag accgttgtct ggaacggccc catgggcgtg
     1081 ttcgagaacc cgaccctggc tgccggcacc ctggctgttg ccaaggccat ggcagagagc
     1141 gacgccacca ccgtcatcgg cggcggcgac tccgcagctg ctgtccagca gatgggcctg
     1201 ggcgacaaga tgacccacat ctccaccggc ggcggcgcat ctctcgagta cctcgagggt
     1261 aaggagctgc ccggcatcgc cgtcatccag aaggcttgat caacaatacc cgcaggagcg
     1321 cggcggcaga gccgccgcgc tttttatgta aaggcttccc cctcaagggg gaaggcttat
     1381 tatggaagga gtaattacca tggataaaca gaaccgcaaa gccatcattg ctggcaactg
     1441 gaaaatgaac aagaccgcta ccgaggccaa ggccctcatc agcgagctga tccccgctgt
     1501 caaggacgcc ggctgcgagg tcgttatctg cgttcccttc accgacctgg tcaccgctgt
     1561 cgagatgacc aagggcacca acatccatgt cggtgccgag aatgtccact ttgagaagtc
     1621 cggcgccttc accggcgaaa tttccgccga tatgctgacc gacctgggcg ttgagtacgt
     1681 cgtgatcggc cacagcgagc gccgccagta ctttgccgag accaacgaga ctgtcaacaa
     1741 gcgcaccaaa gctgcgctgg ccggcggcct gaagcccatc atctgcgttg gcgagagcct
     1801 ggatcagcgt gagcagggcg tcaccgagga actggtccgt atgcaggtca agatcgccct
     1861 gaacggcgtg accgctgacg aactgaagaa cgtcgttatc gcctacgagc ccatctgggc
     1921 catcggcacc ggcaagaccg ctaccgctga ccaggccgag gaagtctgcg ccgccatccg
     1981 caaggtcgtc ggcgagctgt acggcgagga cgccgccaag gccctgaccg tccagtacgg
     2041 cggcagcatg aacccgaaga acgctgagga actgctgaac aagcccgacg ttgacggcgg
     2101 cctgatcggc ggcgcgtccc tgaaggctga ccagtttgct gttatcgtgg acgctgctac
     2161 caaaggctaa attgcgtttt cccggaatgt ttgtagggaa cgcatatatg ccgcccctac
     2221 aaccctatta ttaaggagtt tcaattttat gtccaaaaaa cctgttatgc tctgcattat
     2281 ggacggcttt ggctggacac ccgacaagac cttcggcaat gccgtggccg ccgccaacaa
     2341 gcccaacttg gataagctgt tcgccaacta ccccatgacc accatcgagg catccggcat
     2401 ggctgtcggc ctgcctgatg gccagatggg caactcggaa gtcggccaca ccaacatggg
     2461 cgcgggccgc attgtctacc agcagctgac tctcatcacc aagtccatta aggacggcga
     2521 gatgctgaaa aacccggtgc tggtcaagaa catgaaggcc gctatcgacg caggcaaggc
     2581 catccatctg atgggcctgg tcggcaccgg cggcgtccac agccacgccg accactggta
     2641 tggcgtgctg gaaatggcta agcacatggg cgcgaaggac gtctacctgc actgcatcat
     2701 ggacggccgt gacaccgacc cccacagcgg caagggcttc ctggctgacc tgcaggccaa
     2761 gctggacgag ctgggcatcg gcaagatcgc cagtgtctcc ggccgttact acgccatgga
     2821 ccgcgacaac aactgggacc gtgaggaaaa agcctacgcc gcctttgtct acggcgaggg
     2881 caaccacgcc gccaacgcgc aggaggccat tgaggccagc tacgccgccg acgtgaccga
     2941 cgagttcgtc atccccgttg tcacctgcga gggcggccgc gtcgaggacg gcgacaccgt
     3001 catcttcatg aacttccgcc ccgaccgtgc ccgccagatg acccgcatct tctgcgatga
     3061 cgccttcacc ggctttgagc gccgcggcg
//
LOCUS       DBMDSQ010000236         3085 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_7088_length_3085_cov_1.457440, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000236 DBMDSQ010000000
VERSION     DBMDSQ010000236.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 3085)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3085)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3085
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7088_length_3085_cov_1.457440"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1379
                     /locus_tag="ACZPAX_08215"
     CDS             <1..1379
                     /locus_tag="ACZPAX_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HIR1113919.1"
                     /translation="LKNLGVSHVQLQPIYDYATVDEARPDGGYNWGYDPLNYNVPEGS
                     FATDAFHGEVRVRECRAMVAALHRAGLGVVMDVVYNHTYHGESNLERTVPGYWNRRWP
                     NGMTTNGSGCGCDLASERPMVRKYIVESVLYWATTYHIDGFRFDLMALEDVDTMNAIR
                     AALDSLPGGENILMYGEPWMGGGTNIEGGARPADKRALDALSDRIGFFCDDTRDCIKG
                     NVFDAANAGYVNGAPQHGYDVIHSISAWRNGAHGFWPRRAGQVVQYVSAHDNYTLWDK
                     LAAVARRGDYDWPDADLLAQNRMTAGIYLTCQGLPFMQAGEEWGRTKYGDHNGYKGPL
                     STNRLDWARAHRPEFAALTAFYRGMLAIRRAYPRLSGAAGEPEPFLLALPGWLIGFVP
                     EHDDTAAKGQLAVYYNPERTRQWASLPTGTWRKLSDGVHADASPFGPCFRDALELAPT
                     SVTVLVAE"
     gene            1660..3015
                     /locus_tag="ACZPAX_08220"
     CDS             1660..3015
                     /locus_tag="ACZPAX_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HIR1113920.1"
                     /translation="MSDYAAIEKEARIFTEECVTNNRIDPTLFAQYDIKRGLRDKNGK
                     GVLAGITNISRIDAFEERDGQKVPCEGKLWYRGYNVYDLIRGLRGKRYAFEGAAYLLL
                     LGDLPNKEQLESFTACLAKCRDLPTNFVRDVIMKAPSHDLMNSLTRSVLTLANYDDDI
                     GKTDLQTQLEQCIKLISVFPMLAVYGYHAYNYYECGGSMYIHRPDPSLSTAENLLKML
                     RPDQKYTDLEAHVLDIALLLHMEHGGGNNSTFTTRVVTSSGSDTYSVIAAALSSLKGP
                     KHGGANIKVMKMMDDIRAHVADPLDEEAMADYLGRIVDRQAFDQKGLIYGMGHAVYSL
                     SDPRAVVFKSFVHQLADAKGRSRDFEYYNTIERLAPQVIAEKRHIYKGVSTNVDFYSG
                     FVYDMLGIPVELYTPIFAVARIVGWSAHRMEELVNVDKIIRPAYQSIMQPRDYVPLEE
                     R"
BASE COUNT          612 a          973 c          917 g          583 t
ORIGIN      
        1 atttaaaaaa tctcggcgtc agccatgtgc agctgcagcc catctatgac tacgccaccg
       61 tggatgaggc ccgccccgac ggcggctaca actggggcta tgacccgctg aactacaacg
      121 tgcccgaggg cagctttgcc actgacgcct tccacggaga ggtgcgtgtg cgggagtgcc
      181 gcgccatggt ggccgcgctg caccgcgcgg ggctgggcgt cgtgatggat gttgtctaca
      241 accacaccta ccacggcgag agcaacttgg agcgcaccgt gccaggctac tggaaccgcc
      301 gctggcccaa cggcatgacg acgaacggtt ccggctgcgg ctgcgaccta gccagcgagc
      361 gcccgatggt gcgcaaatac atcgtggaat cggtgctgta ctgggccacg acctaccaca
      421 ttgacggttt ccgatttgat ttgatggcgc tggaagatgt ggacacaatg aacgccatcc
      481 gcgctgcgct ggacagcctg cccggcggcg agaacatcct gatgtacggc gaaccctgga
      541 tgggcggcgg cacgaacatt gagggcggcg cacgccctgc cgacaagcgc gcactcgatg
      601 cgctgagcga taggatcggc tttttctgtg acgatacgcg cgattgtatc aagggcaatg
      661 tctttgatgc cgccaacgcc ggctatgtca acggtgcgcc ccagcacggc tatgatgtca
      721 tccactcgat cagtgcatgg cgcaatggtg cgcacggctt ttggccccgg cgcgccgggc
      781 aggtggtgca gtatgtctcc gcccacgaca actataccct gtgggacaag ctggccgccg
      841 tggcccgccg cggggattat gactggcccg atgccgacct gctggcgcag aaccgcatga
      901 cggcaggcat ctacctgacc tgtcaggggc tgccctttat gcaggcgggc gaggaatggg
      961 gccgtaccaa gtatggcgac cacaacggct acaagggccc tctcagcaca aaccggctgg
     1021 actgggcccg cgcccaccgc ccggagtttg ctgcgctgac ggccttttac cgcggtatgc
     1081 tggccatccg ccgcgcctac ccgcgcctga gcggcgcggc aggggagccg gagccgttcc
     1141 tgctcgcgct gccgggatgg ctcatcggct ttgtgccgga gcatgacgac actgctgcca
     1201 agggtcagct ggctgtctac tacaaccccg agcgcacccg ccagtgggcc agtctgccca
     1261 ccggcacatg gcgcaagctc agtgacggtg tccatgcgga cgcgtcaccg ttcggcccct
     1321 gcttccgcga tgcccttgag ctggctccga ccagcgtgac ggtgctggtg gcagaataac
     1381 aatttacagt ctgacgggag aggaatgacc tctcccgttt tttgtatccc ggtaggggcg
     1441 gggcatgccc cgccctcaac catcccgtaa atgtccgttt tccattacgc cgagggccgg
     1501 gcatgcccgg cccctaccgt gtggtttgtt cacaaattat ccatttgaca gattgttcga
     1561 ttttccgtat aattattctt atacgatttg taaagaatcc tgcatctgct acgatttgtt
     1621 tacccaccgc ggacaaaaag ggaaagggag gccgtcacca tgagcgacta cgcagccatt
     1681 gaaaaggaag cccgcatttt taccgaggaa tgtgtcacca acaaccgcat tgatcccacg
     1741 ctgtttgccc aatacgacat caagcgcggt ctgcgcgaca aaaacggcaa gggcgtgctg
     1801 gcgggcatca cgaacatttc ccgcatcgac gcctttgagg agcgcgacgg ccagaaggtt
     1861 ccctgcgagg gcaagctctg gtaccgcggc tacaacgtct atgacctgat ccgcggtctg
     1921 cgcggcaagc gctatgcgtt tgagggcgcg gcctatctgc tgctgctcgg cgacctgccg
     1981 aacaaagagc agctcgagag tttcaccgcc tgccttgcca aatgccgcga cctgccgaca
     2041 aactttgtgc gcgacgtcat catgaaggcc cccagtcacg acctgatgaa ctcgctgacc
     2101 cgcagcgttc tgacgctggc caactacgat gacgacatcg gcaagaccga cctgcagacc
     2161 caactggaac agtgcatcaa gctcatcagc gttttcccga tgctggcggt ctacggctac
     2221 cacgcctaca attactatga gtgcggcggc agcatgtaca tccaccgccc cgacccgagc
     2281 ctgtccacgg cggaaaacct gctgaagatg cttcgccccg accagaagta caccgacctc
     2341 gaggcccacg tgctggacat tgccctgctg ctgcacatgg agcacggcgg cggcaacaac
     2401 tcgaccttca cgacccgcgt cgtcacctcg tccgggtcgg atacctactc cgtcatcgcg
     2461 gcggcgcttt ccagcctgaa gggccccaag cacggcggcg cgaacatcaa ggtcatgaag
     2521 atgatggacg acatccgcgc ccacgtcgcc gacccgctgg acgaggaggc catggctgat
     2581 tatctgggcc gcattgtgga ccgccaggcg ttcgaccaga agggcctgat ctacggcatg
     2641 ggccatgcgg tctactcgct gagcgacccg cgcgcggtgg tgttcaagag ctttgtccac
     2701 cagctggccg acgccaaggg ccgcagccgc gactttgagt attacaatac gatcgagcgg
     2761 ctggccccac aggtcattgc cgagaagcgc catatttata agggcgtctc gacgaacgta
     2821 gacttctact ccggctttgt gtatgacatg ctgggcatcc cggtggagct gtacaccccg
     2881 atctttgcgg ttgcgcgtat cgtcggctgg tctgcccacc gcatggagga gcttgtcaat
     2941 gtggacaaga tcatccgccc cgcctaccag agcatcatgc agccgcgtga ctacgtcccg
     3001 ctggaagaac gctgaaaata aaaccgggaa gcgccgtttt gacgcttccc ggttttgctt
     3061 atcggtggat atacatacgg gttat
//
LOCUS       DBMDSQ010000237         3041 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_7209_length_3041_cov_1.787415, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000237 DBMDSQ010000000
VERSION     DBMDSQ010000237.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 3041)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3041)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3041
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7209_length_3041_cov_1.787415"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            70..2022
                     /locus_tag="ACZPAX_08225"
     CDS             70..2022
                     /locus_tag="ACZPAX_08225"
                     /inference="COORDINATES: protein motif:HMM:NF013235.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HIR1113921.1"
                     /translation="MVPKDKTSSPIDSLYPNAPIRGPKKSPGRPPKKDPAKAQVEAPV
                     ATYSGRGSQTIVRKSNDLIQNAMSTLTLSQQKLMLHIFAMIKPSDTELPRYEMSIYEF
                     LKLCGVDPHNGSMYAQVRNNIDAIANAKVQWIRLAGTQKITMFRWLSGATIDEGTGKI
                     VLTLDQALKPHLIQLKEFYTTMNITYTLPMKSQYSLKIYELCKSYQNLYLTKKQKGEP
                     LVWNIETLKKQVDCNATNWAHVRRTVLDKAKSEINGHTDIYFDYEVYEKDRQRVIAVA
                     VTIEPVDKQVADDKLNEITKSMSSKSRGKKKLTPAETGSLDDDPSILTLEYVSVPETT
                     IPYSYGATPDLLRQELEVKAELDKLNRELTADEMEAVHTMIGAMVKMAGTPRGNDKMI
                     DGGNAHFFQTVNNVIDNCGGLRRWFEGAAARYATRVIPVARTKSAPLPYLSKAILDDL
                     ENYRMYVTSGMPEPLGGYDAAPEQPDIVETTDFVETDAEPVNQLVPEDAATKKQVTAA
                     LLRYIDRDALSARLTTGQMEAFDDILQMTAYFCRRNVKGKDDGMMEGKANMQFLNALN
                     KVIARYESLTPLYEAMAVMMDYDTYWKDLMKNPKIKNPKLVFQSEVERALLMPAAVIG
                     EYTARKAQNDPRAVHKEYDWVKAFEE"
     gene            complement(2131..>3041)
                     /locus_tag="ACZPAX_08230"
     CDS             complement(2131..>3041)
                     /locus_tag="ACZPAX_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005927447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HIR1113922.1"
                     /translation="GFLGSGKTTFLRKYVQYLVAQGHNVCILENDFGAVNVDAMLLQD
                     LIGDNCDLETISGGCDCDTHQRRMRTKLISMAMRGFDRVVIEPSGIFDVDEFYDILRD
                     EPLDHWYRLGSVIAIVDAKLESELSPQADYLLASEAASAGLVVMSRVQQAGAGECAAV
                     VDHLNHALEGCHCARRFGEDVLCKDWAALTDADFARIDGCGWRQTSYVKLHFDEHEAF
                     TSLYFLKAGRTVEQIKAAAQLLLHDAKYGHILRIKGFIPDGGGWVELNAARDTMTVQK
                     IAQGQEVLIVIGEGLEKEAIERVIKG"
BASE COUNT          725 a          926 c          841 g          549 t
ORIGIN      
        1 caataattga cacttgacgc acaatctgct atcatacgtg taatgtgttg aagaagatga
       61 ggtcacgcca tggtacccaa agacaaaact agttccccca tagattcgct ataccccaac
      121 gccccgatac gcggaccgaa aaaatccccc ggccgcccgc cgaagaagga ccccgccaaa
      181 gcccaggtcg aggccccggt tgccacatac agcggccgcg gcagccagac cattgtgcgc
      241 aaatccaatg acctgatcca gaacgccatg tccaccctga cattgagcca gcaaaaattg
      301 atgctgcaca tttttgcaat gatcaagccg tcggataccg agctgccccg gtacgaaatg
      361 tccatctatg agtttctgaa gctctgcggc gtcgacccgc acaacggctc gatgtatgcc
      421 caggtgcgca acaacatcga cgcgatcgct aacgcgaagg tgcagtggat acgcctggca
      481 ggcacgcaaa agattacgat gttccgttgg ctttccggtg cgaccatcga cgagggcacc
      541 ggcaagatcg tcttaacgct ggaccaggca ttaaagcccc acctgatcca gctaaaagag
      601 ttctacacca ccatgaacat cacctacacg ctgccgatga agagccagta cagcctgaaa
      661 atttatgagc tgtgcaagag ctaccagaac ctttatctca cgaaaaagca aaaaggcgag
      721 ccgctggtct ggaacatcga gacgctgaaa aagcaggtgg actgcaacgc caccaactgg
      781 gcccacgtgc gccgcactgt gctggacaag gccaagagcg agatcaacgg ccacaccgac
      841 atttattttg actacgaagt ctacgaaaaa gaccgccagc gcgtcattgc cgtggctgtc
      901 acgatcgagc ctgtggacaa gcaggtggcc gacgacaagc tgaacgagat cacgaagtcc
      961 atgtccagca agtcccgcgg caagaagaag ctgacccccg ccgaaaccgg ctcgctggac
     1021 gacgatccga gcatcctgac gctggagtat gtctcggtgc ccgagacgac gatcccctat
     1081 tcctacggtg ctacgccgga tctgctgcgc caggagttgg aggtcaaagc cgagctggac
     1141 aagctgaacc gcgagctgac cgccgacgag atggaggccg tacacacgat gatcggcgcg
     1201 atggtcaaga tggccggtac gccgcgcggc aacgacaaga tgatcgatgg cggcaacgcg
     1261 catttcttcc agaccgtgaa caacgtcatc gacaactgcg gcggactgcg ccgttggttt
     1321 gagggggcgg ccgcccgcta cgcgacccga gtcatcccgg tggcccgcac gaagagcgcc
     1381 cccctgcctt atctgtccaa ggccattctg gacgatttgg aaaattaccg tatgtacgtt
     1441 acttccggca tgccggagcc gctgggcggc tacgatgccg cgcccgagca gcctgatatc
     1501 gtcgagacga ccgactttgt ggagaccgat gccgagccgg tcaaccagct ggtgcccgag
     1561 gatgccgcca cgaaaaagca ggtcacagcg gcgctgctgc gctacatcga ccgcgatgct
     1621 ctgtccgccc ggctgacgac cggccagatg gaggcattcg acgatatttt gcagatgacg
     1681 gcctattttt gccgccgcaa cgtcaaaggt aaggacgacg gcatgatgga aggcaaggcc
     1741 aacatgcagt tccttaacgc gctgaacaag gtcattgccc ggtacgagag cctgaccccg
     1801 ctgtatgagg ccatggccgt gatgatggac tatgacacct actggaaaga cctgatgaag
     1861 aacccgaaaa tcaaaaaccc caagctggtg ttccagtctg aagtggagcg tgcgctgctg
     1921 atgcccgccg ccgtcattgg cgagtacacg gcccgcaaag cccagaacga ccctcgcgcc
     1981 gtccacaagg agtacgattg ggtcaaggca tttgaggaat aaacgcagaa ttttttgaaa
     2041 atgtgcgctg aataacaata tcccccgcca gaaaattccg gcgggggatg tgttttggaa
     2101 aggcgtcccc caagggggaa ggcaaaggga ttacccctta ataaccctct caatcgcctc
     2161 tttctccaga ccttctccaa tcacgatgag aacttcctgt ccttgggcga ttttctgcac
     2221 ggtcatggta tcccgggcgg cgttcagctc gacccagccg ccgccatcgg gaataaatcc
     2281 cttgatgcgc agaatgtgcc cgtactttgc atcgtgcaaa agcagttgtg cggcggcttt
     2341 gatttgctcg accgtgcggc ccgccttgag gaaatacagc gaagtaaatg cctcatgctc
     2401 gtcgaaatgc agcttgacat agctcgtctg ccgccagccg cagccgtcaa tgcgggcaaa
     2461 atcagcgtcg gtcagcgcgg cccagtcttt gcacagcacg tcttcgccaa agcggcgggc
     2521 gcagtggcag ccttccagcg catggttcag gtgatccacc acggcggcac actcccctgc
     2581 cccggcctgc tgcactcggc tcatgaccac cagcccggcg ctggcggcct ccgacgccag
     2641 caggtaatcc gcctggggcg aaagctcgct ctcgagcttc gcgtctacga tagcaatgac
     2701 gctgcccaag cggtaccagt ggtccagggg ctcatcccgc aggatgtcgt agaactcgtc
     2761 cacgtcgaaa ataccgctgg gctcgatgac gacccggtca aagccgcgca ttgccatcga
     2821 gatgagcttg gtgcgcatac ggcgctggtg ggtgtcgcag tcgcagccgc cgctgatcgt
     2881 ttccagatcg cagttgtccc caataaggtc ctgcaaaagc atggcgtcca cgttcaccgc
     2941 gccgaagtcg ttttccagaa tgcagacgtt atgaccctgc gccacaagat actgcacgta
     3001 tttacgcaga aaagtcgtct ttccggaacc caagaacccg g
//
LOCUS       DBMDSQ010000238         3023 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_7265_length_3023_cov_2.021903, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000238 DBMDSQ010000000
VERSION     DBMDSQ010000238.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 3023)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3023)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3023
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7265_length_3023_cov_2.021903"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..355
                     /locus_tag="ACZPAX_08235"
     CDS             <1..355
                     /locus_tag="ACZPAX_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005920739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HIR1113923.1"
                     /translation="PSAPAALGPYSQAVDTGSTVYCSGQLGLDPATGNLADGVQAQTH
                     QALKNLQAVLNEAGLSLDNVVKTTVFVQDLADFGTVNEIYGTYFHGGFPARSCVQIAA
                     LPKNALVEIECIAVR"
     gene            complement(407..1189)
                     /locus_tag="ACZPAX_08240"
     CDS             complement(407..1189)
                     /locus_tag="ACZPAX_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005938624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HIR1113924.1"
                     /translation="MALLEVNGIGKSFGTTKVLRDISFELAEGEALAIIGSSGSGKTT
                     LLRCLNFLETADAGSITVAGETLWSAADTRTQSEEAIRKKRLHFGLVFQNFNLFPQYT
                     ALQNVMLAGQLLAKERPDYKQNKKQILADIETQARQLLAQMGLSDREGHYPHQLSGGQ
                     QQRVAIARALALHPDILCFDEPTSALDPELTGEVLRVLRELAEHKTTMIIVTHEMKFA
                     RDVADRILFMDGGVVVEQGDAKEVIDHPKEERTRQFLASYGK"
     gene            complement(1202..1861)
                     /locus_tag="ACZPAX_08245"
     CDS             complement(1202..1861)
                     /locus_tag="ACZPAX_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HIR1113925.1"
                     /translation="MGEAAVIWGRLTEAFWLNCQLFGLTLLFALPLGLVVSFGSMSKF
                     APLRGVVKTFVWIIRGTPLMLQILVIYLGPGLMGFTSPWPSGGSGRLVAAVVAFAINY
                     ACYFSEIYRGGIEAVPKGQTEAGQVLGMTKAQIFFRVTLLQVIKRILPPMGNEIITLV
                     KDTSLANVIANKDIIMMAKEYSAKGLIWPLFSTAVFFLVFVGALTLLFGWLEKRLDYF
                     K"
     gene            complement(1861..2691)
                     /locus_tag="ACZPAX_08250"
     CDS             complement(1861..2691)
                     /locus_tag="ACZPAX_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005938948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HIR1113926.1"
                     /translation="MKKLISTVLALGMAASLAACGSSASTAASTAESTVESTAAAGDS
                     DLDYIKGKGKMTIGYTVYEPMNYTDADGNFTGFDTELATAVCEKLGVEPEFVEINWDT
                     KIVELDAKSIDCIWNGMTLTDDIQANTACTKAYAKNAQVVVMKADADYSSTADLVGKT
                     VVAEAGSAGESAINDDESLAQAEYVSKSVQTDCLMEVAAGTADAAVLDVTLASAMIGD
                     GTDYANLAIKDELNAEEYGVAFRKGSDAADAVNAAFDELKADGTMQALADKYSLALAD
                     "
BASE COUNT          596 a          998 c          807 g          622 t
ORIGIN      
        1 cccgagcgcc cccgccgccc tcggccccta ttctcaggcc gttgacaccg gctccaccgt
       61 ctattgcagc ggccaactcg gcctggatcc cgccaccggc aatctggccg atggcgtgca
      121 agcccagacc caccaggccc tcaaaaatct gcaggccgtc ctgaacgagg ccggcctgag
      181 cctcgacaac gtcgtcaaaa cgacagtttt cgtgcaggat ctggccgatt tcggcaccgt
      241 gaacgagatt tacggcacct acttccacgg cggcttcccc gcccgcagct gcgtccaaat
      301 cgccgccctg cccaagaacg ccctcgtcga gattgagtgc atcgcggtac ggtaattgcc
      361 cacttttaaa aaaacggcag accgccatcc aacggtctgc cgttttttat ttcccataac
      421 tcgccaagaa ctgtctcgtc cgctcctctt tggggtggtc gatgacctct ttcgcgtcgc
      481 cctgctccac gaccacgccg ccgtccataa acaggatgcg gtcggccacg tcgcgggcaa
      541 acttcatctc atgggtcaca atgatcatcg tggtcttgtg ctcggccagc tcgcgcagca
      601 cccgcagcac ctcgccggtc agctcagggt ccagcgcact cgtcggctcg tcaaaacaca
      661 aaatatccgg gtgcagcgcc aaggcgcggg cgatcgccac ccgctgctgc tgcccgcccg
      721 aaagctggtg cgggtagtgt ccttcgcggt cagaaagccc catctgcgcc agcagctgcc
      781 gcgcctgcgt ctcgatatcg gccaggatct gctttttgtt ctgcttgtag tccgggcgtt
      841 ccttcgccaa aagctggccc gccagcatca cgttttgcag cgccgtgtac tgcgggaaca
      901 ggttgaaatt ctggaacacc agcccgaaat gcagccgctt tttgcggatg gcctcctcgc
      961 tctgggtgcg ggtatcggcg gcgctccaca gcgtctcgcc cgccaccgta atgctgcctg
     1021 cgtccgccgt ctccaaaaag ttcaagcagc gcagcagcgt cgttttgccg gagccggacg
     1081 acccgatgat ggccagggcc tcgccctcgg ccagctcaaa cgagatatcg cgcagcacct
     1141 ttgtggtgcc aaagctcttg ccgatgccgt ttacttccaa caatgccata gccgcgcccc
     1201 cttacttgaa atagtccagc cgcttttcca gccaaccgaa cagcagcgtc agcgcaccca
     1261 cgaacaccag gaagaacacc gccgtggaaa acagcggcca gatcagccct ttggcactgt
     1321 actctttcgc catcataatg atgtctttgt tggcaatgac attcgccagc gaggtatcct
     1381 tgaccagcgt gatgatctca ttgcccatcg gcggcagaat gcgcttgatg acctgcaaaa
     1441 gcgtcacgcg gaagaagatc tgcgccttgg tcatgcccag cacctgccct gcctcggtct
     1501 ggcctttggg gaccgcctcg atgccgccgc ggtaaatttc cgaaaaatag caggcgtaat
     1561 tgatggcaaa cgccaccacc gccgccacca ggcggccgct gccgcccgac ggccaggggc
     1621 tggtaaagcc catcaggccc ggccctaagt agatgaccag aatttgcagc atcagcggcg
     1681 tgccgcggat gatccagaca aaggtcttga ccacgccgcg cagcggggca aacttgctca
     1741 tggagccaaa cgacaccacc agccccagcg gcagtgcgaa caacagcgtc agaccgaaca
     1801 gctggcagtt gagccagaat gcctcggtca ggcggcccca gatgacagcc gcctcgccca
     1861 tcagtcggcc agcgccaggc tgtacttgtc ggccaaagcc tgcatcgtgc cgtcggcctt
     1921 cagctcgtca aaggcagcgt tcacggcgtc ggcagcatcg ctgcccttgc ggaaggccac
     1981 gccgtactcc tcggcgttca gctcgtcttt aatagccagg ttcgcgtagt cggtgccgtc
     2041 accaatcatg gcggaggcca gcgtcacgtc cagcacagcg gcatccgccg taccggcggc
     2101 gacctccatc aggcaatcgg tctggacgga tttgctgaca tactcggcct gggccaggct
     2161 ctcgtcgtcg ttgatggcgc tctcaccggc ggaaccggcc tctgcgacca cggtcttgcc
     2221 gacgaggtcc gcagtggagg aatagtcggc gtcggccttc atcacgacga cctgcgcgtt
     2281 cttggcgtag gccttggtgc aggcggtgtt ggcctggata tcatccgtca gcgtcatgcc
     2341 gttccagatg cagtcaatgc tcttggcatc cagctcaaca attttggtgt cccagttgat
     2401 ttccacaaat tccggctcca cgcccagctt ctcacacaca gcggtcgcca gttcggtgtc
     2461 aaagccggtg aaattgccgt cggcgtcggt gtagttcatc ggctcatata ccgtgtagcc
     2521 gatggtcatt ttgcctttgc ccttgatgta gtccaggtca ctgtccccgg ctgctgcggt
     2581 agattccacg gtgctctcgg ccgtggacgc cgccgtggat gcgctgctgc cgcaggctgc
     2641 caggctggcc gccataccca atgccagaac ggtgctgatc agctttttca tgatgacttc
     2701 ctccttgtat acactttaca caaaccctca caccaccgct ctaaagcggt agcattcttt
     2761 aatagtaatg caaaacctct ttttctgcaa gaccttttgc aaaaaaagag gtgaaaagta
     2821 tatttatgca gttttacgcc cgccccgccg catactgcgg cataaaaaga ataccatgtc
     2881 tcggcagttt ttgcagctgc ggaacatggt attttttatt ttaggcaata ccgcacaatt
     2941 cccgccttac ggcttaagca ccgctccggc ggctgcggca cggcatctgc gttgccaaaa
     3001 tgctcgataa tacacaaagt att
//
LOCUS       DBMDSQ010000239         3006 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_7310_length_3006_cov_1.532530, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000239 DBMDSQ010000000
VERSION     DBMDSQ010000239.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 3006)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3006)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3006
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7310_length_3006_cov_1.532530"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            125..385
                     /locus_tag="ACZPAX_08255"
     CDS             125..385
                     /locus_tag="ACZPAX_08255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113927.1"
                     /translation="MKVHDLLNTCGNGFAAYRFYGRHDDDMIGYWVKRNRIPELLVET
                     KLFTWRVERGRLCLQVDDETLEKVLYTIDGGDGRHNQPISAC"
     gene            399..563
                     /locus_tag="ACZPAX_08260"
     CDS             399..563
                     /locus_tag="ACZPAX_08260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113928.1"
                     /translation="MQIANEYKAQQTAVCSLLGGIVADYFKDADHRAAFESWYLERYG
                     KPYQWKKLEV"
     gene            578..859
                     /locus_tag="ACZPAX_08265"
     CDS             578..859
                     /locus_tag="ACZPAX_08265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113929.1"
                     /translation="MTRKRCIKILMEAIGSSKYRQTEKAFLVCQKIAREDVPNASNEN
                     VLIAMLFQGANMAGDMEDLEIELRCIARGTWLRMRQSMKHRRLFQGGTA"
     gene            856..1143
                     /locus_tag="ACZPAX_08270"
     CDS             856..1143
                     /locus_tag="ACZPAX_08270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113930.1"
                     /translation="MKIFDLLNTMRRVPQYVGVHGPGLVLEHTAWTDWHAMPKDVLKA
                     TVMTWWIDNEMDELRIAVHPATAAKLLEEYKVGENVQQIIPIAHNKKELKL"
     gene            1140..1292
                     /locus_tag="ACZPAX_08275"
     CDS             1140..1292
                     /locus_tag="ACZPAX_08275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113931.1"
                     /translation="MSDKENRSRNLLEQLEKLPEKAQEQIGHVIQGAALVTEAREKEN
                     KNGGAA"
     gene            1289..1570
                     /locus_tag="ACZPAX_08280"
     CDS             1289..1570
                     /locus_tag="ACZPAX_08280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113932.1"
                     /translation="MKRGDRVDKKTAAVQEIVENAVQSKRLYRKTLHETNGVRNAGTR
                     GALICRAAELAGMVQAYRECLLLMQRGGMIPPELELFVGAMLDDSPKQQ"
     gene            1923..2150
                     /locus_tag="ACZPAX_08285"
     CDS             1923..2150
                     /locus_tag="ACZPAX_08285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113933.1"
                     /translation="MKITEVDKPTISVQMACRVLRGSGVQMGVEKMRAGIAQGVYPFG
                     EIVTRGEHLKNDCYTVYTKLLADWMKERGIV"
     gene            2147..2368
                     /locus_tag="ACZPAX_08290"
     CDS             2147..2368
                     /locus_tag="ACZPAX_08290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113934.1"
                     /translation="MKYAKEFLVGVWVVGKLCGVYLLVLAVAMLGSMHGGVAAVLGAV
                     MTAVCCAVGGLTLFCMGQAAKQILQEGRH"
     gene            2368..2685
                     /locus_tag="ACZPAX_08295"
     CDS             2368..2685
                     /locus_tag="ACZPAX_08295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113935.1"
                     /translation="MAENKFSREELDIMKWDWIHCRNVQQVAELHACRKQDVIDALNL
                     DEKPRPARKRRWEELHPAKAQRFVKQVESGVSMAKASREAGIRSAHTGYVVYRRMTQE
                     SWM"
     gene            2824..>3006
                     /locus_tag="ACZPAX_08300"
     CDS             2824..>3006
                     /locus_tag="ACZPAX_08300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113936.1"
                     /translation="MIMQQCLYTFKITHPECRACNGLKATCESYVPNPSEKARREIDP
                     DARKKLPEKLFPAGMQG"
BASE COUNT          747 a          753 c          980 g          526 t
ORIGIN      
        1 gccaagggta tgataccgcc gcacggcaag acaaatttta aattcagtat cagtcaatag
       61 aaacacaacc tttctgccgc cattgtaaca cagcggggcg gtgtgggcaa ggaggaaaac
      121 caagttgaaa gtccatgact tgttgaatac ctgcggcaat gggtttgccg cgtacagatt
      181 ttacggacgg catgacgacg acatgatcgg ttattgggtg aaacgcaacc gaatcccgga
      241 gttgcttgtg gaaacgaagc tgtttacatg gcgcgttgag cgcggccggc tgtgcttgca
      301 ggtggatgat gaaacgctgg aaaaggtgct gtacaccata gacggcgggg atggccgaca
      361 caatcagcct atttccgcct gctgaaagag gtgaaaaaat gcagattgca aacgaataca
      421 aggcccagca gacggcggtc tgctctttgc tgggcggtat cgtggcggac tactttaagg
      481 acgcggacca ccgggccgcg tttgaaagct ggtatctgga acgatacgga aagccgtacc
      541 aatggaagaa gctggaagtt taagtggagg taaagctatg acacggaaac gctgcattaa
      601 aattttgatg gaggctatcg gcagttccaa gtacaggcaa accgagaaag cctttctggt
      661 atgccagaag attgcccggg aggatgtgcc gaacgcaagc aacgagaatg tacttattgc
      721 catgctgttc caaggcgcga acatggcggg cgacatggaa gatttggaaa ttgaactgcg
      781 ctgcattgcg cggggaacgt ggctgcggat gcgccagtcc atgaagcacc gcaggctgtt
      841 ccaaggcggc acggcatgaa aatttttgac ctgctgaaca ccatgcgccg cgtgccgcag
      901 tatgtgggcg tgcatgggcc gggtcttgtg ctggaacaca cggcctggac agactggcac
      961 gccatgccga aagatgtgct gaaagccacg gtcatgacct ggtggattga caacgaaatg
     1021 gacgaactgc gcattgccgt ccaccccgcc acggcggcaa aactgctgga agaatacaaa
     1081 gtgggtgaaa atgtccaaca gataatccct attgcccaca acaaaaagga gctgaaatta
     1141 tgagtgacaa ggaaaatcgt tcccggaacc tgttggagca gctggaaaag ctgcccgaga
     1201 aggcgcagga gcaaatcggc catgtgatcc agggcgcggc cctggtgacg gaggcacgcg
     1261 agaaggaaaa caagaacggc ggcgcagcat gaaacgaggc gaccgagtag acaagaaaac
     1321 cgccgccgta caggaaatcg tagaaaatgc ggtgcagagc aagcgactgt accgaaagac
     1381 cctgcacgag acaaacggcg tgcgcaatgc cggaacacgc ggggcgctaa tctgccgcgc
     1441 ggccgagctg gcgggcatgg tgcaggcgta ccgcgaatgt ctgctgctga tgcagcgcgg
     1501 cggcatgata ccgccggaac ttgaactgtt cgtcggtgcc atgctggacg attcccctaa
     1561 acagcaatga ccccgccccg gcggggcata tgagccgccg acggctgcaa gaggcccggg
     1621 gcggatggtc catgcaatgg acctcacaac ctttctcccg cggctgcccg gcgggtaagt
     1681 tcggcagccc ccggctgtgc ggggaatgca caggcgcggt gctctcttta ctttctctgc
     1741 accgcacaag aggcagcgca gcgggcctgt tccgcgttgc gcaaagcctg gccgcccggt
     1801 cccctatacc gggcggcaca catgaggtgc gccgatggtt cgcgggcggg ctcgtgaccc
     1861 gaccgatgcg gttcaattcc gcagcgcacc accaaaggaa caaacacaaa ggagcgcgga
     1921 acatgaaaat caccgaagtg gacaagccga cgatatcagt acagatggcg tgcagggttt
     1981 tgagaggatc cggcgtgcag atgggcgttg aaaagatgcg ggccggtatc gcccagggcg
     2041 tctacccttt cggcgagatt gtgacgcgcg gcgaacatct gaaaaatgac tgctacacag
     2101 tgtacacaaa actgctggca gactggatga aggagcgggg tatcgtatga aatacgcgaa
     2161 ggaatttctt gtgggcgtct gggtcgtggg caagctgtgc ggtgtgtacc tgctggtgct
     2221 agccgtggct atgctgggca gtatgcacgg cggcgtggcc gctgtgctgg gcgcggtgat
     2281 gacagccgtg tgctgcgccg tgggcgggct gacactgttc tgcatgggcc aggctgccaa
     2341 acagattttg caggaggggc ggcactgatg gcggaaaaca agttcagccg cgaagaactg
     2401 gacatcatga agtgggactg gatacattgc aggaacgtcc agcaggtggc cgagctgcac
     2461 gcttgcagaa agcaggacgt tatcgacgcc ctgaatcttg acgaaaagcc gcggcccgcg
     2521 cggaaacgcc gctgggaaga attgcacccg gccaaggcgc agcggtttgt gaagcaggtt
     2581 gaaagcggcg tatccatggc aaaagcaagc cgcgaagcag ggatacggag cgcccatacc
     2641 ggctatgtcg tgtataggcg gatgacacag gaaagttgga tgtgatttaa caaagtagtg
     2701 gaacgacgtg aagattgaaa ttattgcaga accgcccggc ggggcgtggt atctgcggtg
     2761 gatgcaccgg gcgcggcgct gtccacgcgg cgacaccacc tgcggaagga tggaaccagt
     2821 tgaatgatta tgcagcagtg tttgtacaca tttaaaatca cgcacccgga gtgccgggcc
     2881 tgcaatgggc tgaaggcgac gtgtgagagc tacgtgccga acccaagcga gaaggcccgg
     2941 cgtgagattg acccggatgc gcggaagaag ttgccggaaa agctcttccc tgccggtatg
     3001 cagggc
//
LOCUS       DBMDSQ010000240         3006 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_7311_length_3006_cov_1.364544, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000240 DBMDSQ010000000
VERSION     DBMDSQ010000240.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 3006)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3006)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3006
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7311_length_3006_cov_1.364544"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     misc_binding    complement(58..303)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(343..1659)
                     /locus_tag="ACZPAX_08305"
     CDS             complement(343..1659)
                     /locus_tag="ACZPAX_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HIR1113937.1"
                     /translation="MNIILLSGGSGKRLWPLSNDIRSKQFIKIFKTVDGYESMVQRVY
                     RQIKEVDAEANVTIATSKTQVSSIHNQLGSAVGVCVEPCRRDTFPAIALATAYLHDVK
                     GVGLDDAVVVCPVDPYVNNDYFAALKGLSDLAEQGNANLSLMGIEPTYPSEKYGYIIP
                     ESADAVSSVKTFKEKPDAATAEKYIARGALWNGGVFAYKLRYVLDKAHELIDFTDYHD
                     LFNKYETLKKISFDYAVVENESKIQVMRFAGQWKDMGTWNTLTEAMEEPSIGKAILND
                     TCEGVNVLNELDVPVLCMGLKNVVVSASPEGILVSDKEQSSYIKPYVDSIHQQIMFAE
                     KSWGSFRVLDIEDESMTIKITLNPGHGMNYHSHENRDEVWTIISGTGRAILDGAEKQV
                     KAGDILTMQAGCKHTIFADTELKIIEVQLGKAISVEDKQKYPLPQA"
     gene            complement(1671..2615)
                     /locus_tag="ACZPAX_08310"
     CDS             complement(1671..2615)
                     /locus_tag="ACZPAX_08310"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014424865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase family protein"
                     /protein_id="HIR1113938.1"
                     /translation="MMEKNAKIYVAGHRGMVGSAIVRELQRQGYTNIITRTHKELDLC
                     RQEDVEKFFAEEKPEYVFLAAAKVGGIVANQEALADFMWFNMTLEMNVIHSAWQNGCK
                     KLEFLGSSCIYPRMAPQPMKESCLLTSELEKTNEAYALAKISGLKYCEFLNRQYGTDY
                     ISVMPTNLYGPNDNYHPTHSHVVPALIRRFHEAKINGVDSVTCWGDGSPLREFLYVDD
                     LANLCVFLMNNYSGNETVNAGTGKELTIKELTELVAKVVGYTGKIKWDTSKPNGTPRK
                     LLDVSKATNLGWTYKTELEDGLRLSYEDFLHNPMRAER"
     gene            complement(2640..>3006)
                     /locus_tag="ACZPAX_08315"
     CDS             complement(2640..>3006)
                     /locus_tag="ACZPAX_08315"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HIR1113939.1"
                     /translation="DFVIATGEQHSVREFCQLAFHDVGIELEFTGEGMNEKGIDKATG
                     RVIIEVSPDFYRPTDVVNLWGDPSKAKRELGWNPTKTTFEELVKIMVDHDMKKVAVER
                     AEEHIQTNLAEYLEKGVIK"
BASE COUNT          580 a          840 c          860 g          726 t
ORIGIN      
        1 tacacctctg tttgactttg ggccggagca aataaaaaag ccccgtccct gtgtgaaatc
       61 agggacgaag ccttgtacat acaattctcc gcggtaccac ccgtgttccg catataaaaa
      121 gcggcactct ttgctgcggg taacggtgca gggccgttgc gcactaccgg gccggaacgg
      181 cctttcccgc gcaccactcg ggagggaact tccgcacaat ttctttgcag gcggcttgca
      241 gccgacgacc gccctctctg gcccaaagaa ccttgcgcgt actttctccg tcattgtgtt
      301 tttgcgaata ctagattagt atagcgcgaa ttttgcgcac tgtcaagcct gcggcagcgg
      361 atacttctgc ttatcttcca cgctgatagc tttgccgagc tgcacctcaa tgattttcag
      421 ctcggtatcg gcaaagatcg tgtgcttgca gcctgcctgc atggtcagga tatcccctgc
      481 cttgacctgc ttctctgcac cgtccagaat cgcgcggccg gtgccggaaa tgatagtcca
      541 gacctcgtcg cggttttcgt ggctgtggta gttcatgccg tggccagggt tgagggtgat
      601 tttgatggtc atgctttcat cctcaatgtc caacacgcgg aaggaacccc aggacttttc
      661 ggcaaacata atttgctggt gaatgctgtc cacatagggc ttgatgtagc tggattgttc
      721 cttgtcggag acgaggatgc cctccgggct ggcggagaca acgacgtttt tcagccccat
      781 gcagagaacc ggcacgtcca gctcgttcag cacattgacg ccctcgcagg tgtcgttcag
      841 gatcgccttg ccgatgctgg gttcttccat ggcctcagtc agtgtgttcc aggtgcccat
      901 gtccttccac tggcccgcaa agcgcatgac ctggattttg gactcattct cgacaacggc
      961 gtagtcgaag ctgatttttt tcagcgtctc gtacttattg aacaagtcgt gatagtcggt
     1021 aaagtcgatc agctcgtggg ctttgtccag cacatagcgc agcttgtaag caaaaacgcc
     1081 gccgttccag agcgcccccc gggcgatata tttttccgcc gtggcggcgt caggcttctc
     1141 tttgaaagtc ttgaccgagc tgacggcgtc ggcactctcg ggaatgatgt agccgtactt
     1201 ttcgctgggg taagtcggct caatgcccat cagagagagg ttcgcgttcc cctgctcggc
     1261 caggtcagac aagcctttga gagcggcaaa atagtcattg ttgacatagg gatccaccgg
     1321 gcagacgaca acagcgtcat ccagtccgac gcccttgaca tcgtgcaggt aggccgtcgc
     1381 cagtgcgatg gccgggaagg tatcgcggcg gcacggctca acgcagacac ccaccgcact
     1441 gcccagctgg ttgtggatgg acgacacctg ggtcttgctg gtggcaatcg tcacattggc
     1501 ctcggcgtcc acctctttga tttggcggta gacacgctga accatcgatt catagccatc
     1561 gacggtcttg aaaattttga tgaactgctt ggaacggata tcgttggaca gcggccacag
     1621 acgcttgccg gagccgccgg acagcaggat aatgttcatg gcgtttcctt tcagcgctcg
     1681 gcgcgcatgg ggttatgcag gaaatcctca taggaaaggc gcaggccgtc ttccaactcg
     1741 gtcttatagg tccagcccag gttcgttgcc ttgctgacgt ccagcagctt gcggggcgtg
     1801 ccgttgggct tggaggtatc ccacttgatt ttgccggtgt agcccacgac cttggcgaca
     1861 agctcggtca actccttgat ggtcaactcc ttgccggttc ccgcgttgac ggtctcattg
     1921 ccggaatagt tgttcatcag gaacacgcag agattggcaa ggtcgtcgac gtataaaaac
     1981 tcgcgcagcg ggctgccgtc accccaacag gtcacagagt ccacgccgtt gattttggct
     2041 tcatggaagc ggcggatgag tgccggcacc acatggctgt gggtcggatg gtagttgtcg
     2101 ttggggccat agaggttcgt cggcatgacg gagatgtagt cggtgccgta ctggcggttc
     2161 agaaactcgc agtatttcag gccgctgatt ttggcgagag cgtaggcctc gttcgtcttt
     2221 tccagctcgg aggtcaacag gcagctttcc ttcatcggct ggggtgccat gcgggggtag
     2281 atacaggaag aacccaaaaa ttccagcttt ttgcagccgt tctgccaggc agagtggatg
     2341 acgttcattt cgagcgtcat gttgaaccac atgaaatcgg ccagggcctc ctggttggcg
     2401 acgataccgc cgaccttggc ggctgccaga aagacatact cgggcttttc ctcggcaaag
     2461 aacttctcga catcctcctg gcggcagagg tcaagttcct tgtgggtgcg ggtgatgata
     2521 ttggtgtatc cctggcgctg caattcgcgc acgatggccg accccaccat gccgcggtgg
     2581 ccggcaacgt agatttttgc attcttttcc atcataataa aataccttct cccgataaat
     2641 tatttgataa cgcccttttc caggtactcg gcaaggttcg tctggatatg ctcctcggcg
     2701 cgctcgacgg cgaccttctt catgtcgtgg tcgaccatga tcttgacgag ctcctcgaac
     2761 gtcgtcttgg tggggttcca gcccagctcg cgcttggcct tgctggggtc gccccacagg
     2821 ttgacgacgt cggtggggcg gtagaagtcg ggactgacct caataatgac cctgccggtg
     2881 gccttgtcga tgcccttctc gttcatgccc tcgccggtaa attccagctc gatgcctaca
     2941 tcgtggaagg ccagctggca gaactcgcgg acggaatgct gctcgccggt ggcaatgacg
     3001 aaatcc
//
LOCUS       DBMDSQ010000241         2981 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_7388_length_2981_cov_1.686806, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000241 DBMDSQ010000000
VERSION     DBMDSQ010000241.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 2981)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2981)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2981
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7388_length_2981_cov_1.686806"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..684
                     /locus_tag="ACZPAX_08320"
     CDS             <1..684
                     /locus_tag="ACZPAX_08320"
                     /inference="COORDINATES: protein motif:HMM:NF013614.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufB/SufD family protein"
                     /protein_id="HIR1113940.1"
                     /translation="VSASLTRIRAAENAHVTLVQVQLLGNRARRWNAVAIEQGKSARV
                     EQVRIELGGCVVACGARTLLNHNKCEYDLDAVYFGHGNDVLDFNDVSVHTGRDTLCEM
                     HTAGVLAGSADKILRGTVDFQRGAKRGVGHESEDVLLFSPNARNRTAPLILCGEEEVE
                     GQHAASVGRLDENKLYYLRSRGLSEAQARRLMVDARFAPAIDKIPLESLQDEVRENVA
                     GRLNDELDG"
     gene            668..1879
                     /locus_tag="ACZPAX_08325"
     CDS             668..1879
                     /locus_tag="ACZPAX_08325"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004623946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HIR1113941.1"
                     /translation="MNWTADFPILAADENGKRLVYLDSAATTQHPTQVLSAVVNYYNK
                     ENANPHRGVYELAMRATDAHEGARHNVAQFFNAEDDEIVFTQNTTESLNLVAYSYGMN
                     FLHEGDEIVISVAEHHSNMVPWQRVAKATGAKLVYMYPGENGRLTTEELDKKITSKTK
                     VVAVAMVSNVLGLRAPVEEIVKRAHAVGAVVVLDCAQSAPHTPVDVKKLDVDFAACSA
                     HKLYAPMGVGALYARAELLEKMPPFLSGGDMIAAVHESGATWADGPRKFEAGTRNVGG
                     EVGFAAAIDYMNGIGWQAMEAHEHMLLDRMLAGMRAMPWLTVYGEPVAEGRYGVVSFN
                     VNEVHPHDVATILDAGGVAVRAGHHCAQPLMEFLGIGSCCRASVAIYNTPEDVDALLE
                     NLENVRKVMGL"
     gene            1876..2337
                     /gene="sufU"
                     /locus_tag="ACZPAX_08330"
     CDS             1876..2337
                     /gene="sufU"
                     /locus_tag="ACZPAX_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004603603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly sulfur transfer protein
                     SufU"
                     /protein_id="HIR1113942.1"
                     /translation="MNLNELYTQIITENSRSKENRHPVEGATHSLEGVNPSCGDDITL
                     QLRVKNGKIEDAGFIGSGCAISQASASLMIDLVKGRTVKDAHRLISLYFQMIKGKITP
                     EELDDLEDVAALQGIAHVPARVKCAVLAWHTLEEALDGEGKTDKVTTEDKE"
     gene            2521..>2981
                     /locus_tag="ACZPAX_08335"
     CDS             2521..>2981
                     /locus_tag="ACZPAX_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007048395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HIR1113943.1"
                     /translation="MCGFVGFTGLREQREAILHKMADRIIHRGPDMEGYHISGDDPRT
                     AIALGFRRLSIIDLAAGKQPMYNEDGTVVCVFNGEIYNFMDLRAELQAKGHIFKTHCD
                     TEVIIHGYEEYGTKLAGMLRGMFAFVVWDTKTNEMYGARDIFGIKPFYYTQT"
BASE COUNT          615 a          913 c          897 g          556 t
ORIGIN      
        1 gtttccgcca gcctgacccg catccgcgct gccgaaaacg cccatgtaac tttggtgcag
       61 gtgcagctgc tgggcaaccg cgcccgccgc tggaacgctg ttgccattga acagggcaaa
      121 tccgcccgcg tcgagcaggt gcgcatcgag ctgggcggct gtgtcgtcgc ctgcggtgcc
      181 cgcaccctgc tgaaccacaa caagtgcgag tacgacctgg acgccgtcta tttcggccac
      241 gggaacgatg tgctcgactt caacgatgtc tccgtccaca ctggcaggga caccctgtgc
      301 gagatgcaca ccgccggtgt gctggccggc agcgccgata aaattctgcg cggcacggtg
      361 gattttcagc gtggtgccaa gcgcggcgtc ggccacgaga gcgaggacgt gctgctgttc
      421 agccccaacg cccgcaaccg taccgcgccc ctcatcctct gcggtgagga ggaagtcgaa
      481 ggccagcatg ccgcgtccgt gggccgcctg gacgagaaca agctgtacta tttgcgctcc
      541 cgcggtttga gcgaggcgca ggcccgccgc ctgatggtgg acgcccgctt tgccccggcc
      601 atcgacaaga tcccgctgga atctttgcag gacgaagtac gtgaaaatgt tgcagggagg
      661 ctgaacgatg aactggacgg ctgatttccc cattctggct gccgacgaga acggcaagcg
      721 ccttgtctac ctggacagcg ccgccaccac ccagcacccc acccaggttc tgagcgcggt
      781 ggtcaactat tataataaag agaacgcaaa cccccaccgc ggcgtctatg agctggccat
      841 gcgtgccacc gacgcccacg agggtgcccg ccataacgtg gcgcagttct ttaatgccga
      901 ggacgacgaa attgtgttca cccagaacac gacggagagc ctgaaccttg tcgcgtacag
      961 ctacggcatg aatttcctgc acgaaggcga cgaaatcgtc atttccgtgg ccgagcatca
     1021 ctccaacatg gtaccctggc agcgcgtggc gaaggccacc ggcgcaaagc tcgtttatat
     1081 gtatcccggc gagaacggcc gcctgacgac cgaggagctg gacaagaaaa tcaccagcaa
     1141 aaccaaggtt gtcgccgtgg cgatggtctc caacgtcctg ggcctgcgtg cccccgtgga
     1201 ggagattgtc aagcgtgccc atgccgtggg tgccgtcgtc gtgctggact gcgcccaaag
     1261 cgccccacac accccggtgg atgtcaaaaa gctggacgtg gattttgcgg cctgctctgc
     1321 ccacaagctg tatgcgccga tgggcgtggg tgccctgtac gcccgcgccg agctgctgga
     1381 aaagatgccg ccgttcctgt ccggcggcga tatgatcgcc gccgtgcatg agagcggtgc
     1441 cacctgggcc gacggtcccc gcaagtttga ggccggcacc cgcaacgtcg gcggtgaggt
     1501 cggctttgcc gccgccatcg actacatgaa cggcatcggc tggcaggcca tggaagccca
     1561 cgagcacatg ctgctggatc gcatgctggc cggtatgcgt gccatgccgt ggctgaccgt
     1621 ctacggcgag ccggtggccg agggccgtta cggcgtcgtc agcttcaacg tcaacgaggt
     1681 acacccccac gacgtggcga cgattctgga tgcgggcggc gtggccgtgc gcgccgggca
     1741 tcattgtgcc cagcccttga tggaattcct gggcatcggc agctgctgcc gcgccagcgt
     1801 ggcgatctac aacacgcccg aggacgtgga cgccctgctg gaaaatcttg aaaatgtcag
     1861 aaaggtgatg ggcctgtgaa cctgaacgaa ctgtataccc agatcattac cgagaacagc
     1921 cgcagcaagg agaaccgcca cccggtcgag ggggcgaccc acagcctgga gggcgtgaac
     1981 cccagctgcg gcgacgatat cacgctgcag ctgcgcgtga aaaacggcaa aattgaggac
     2041 gccggtttca ttggctccgg ctgcgcgatc tcccaggctt cggcttcgct gatgatcgac
     2101 ctggtcaagg gccgcaccgt caaggatgcg caccgcctca tcagcctgta tttccagatg
     2161 atcaagggca agatcacgcc ggaggaactg gacgatttgg aggatgttgc cgctttgcag
     2221 ggcatcgccc acgtccccgc ccgtgtcaag tgcgccgtgc tggcatggca caccctggaa
     2281 gaagcgctgg acggcgaagg caagaccgat aaggttacga ccgaggataa agagtaattg
     2341 taatgcaggg gctttgctac ctcgcccctg ccacccacat aaccctcgca agggcgcagc
     2401 attccgctgc gtcctttttt ctttacgacc acttttcttt tcctctattc tgtggtacaa
     2461 tatacaaata tataagttaa aatttattat tgtgcctata tagagaaagg gaaatttacc
     2521 atgtgtggtt ttgttggctt taccggtctg cgtgaacagc gcgaggccat cctgcacaaa
     2581 atggcggacc gcatcatcca ccgcggtccc gatatggaag gctaccacat cagcggcgat
     2641 gacccgcgca cggccattgc gctgggcttc cgccgcctga gcatcattga cctggccgcg
     2701 ggcaagcagc cgatgtacaa cgaggacggc accgttgtct gcgtgttcaa cggcgaaatt
     2761 tacaacttta tggatctgcg tgccgaattg caggccaagg gccatatctt caagacccac
     2821 tgcgacaccg aggtcatcat ccacggctat gaggagtacg gcacaaagct ggccggtatg
     2881 ctgcgcggca tgtttgcctt tgtcgtgtgg gataccaaaa cgaacgagat gtacggcgcg
     2941 cgtgatattt tcggcatcaa gccgttctac tacacccaga c
//
LOCUS       DBMDSQ010000242         2980 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_7392_length_2980_cov_1.902397, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000242 DBMDSQ010000000
VERSION     DBMDSQ010000242.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 2980)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2980)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2980
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7392_length_2980_cov_1.902397"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..508)
                     /locus_tag="ACZPAX_08340"
     CDS             complement(<1..508)
                     /locus_tag="ACZPAX_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008979936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HIR1113944.1"
                     /translation="MDTKKKRRVVITGLGTVNPTGNTVAESWAAVKAGRCAVGPITAF
                     DATDFKVKLAAEVKDFDPAERIDKREARKMARFTQFAVAAAEEAVRDSGLALEEIDRA
                     RFGVILSSGIGGLPTIEEEHTRGQQRGFEKVSPYFVPMAIGNMAAGQIAIRFGLQGMC
                     SCPTTACAG"
     gene            complement(517..1269)
                     /gene="fabG"
                     /locus_tag="ACZPAX_08345"
     CDS             complement(517..1269)
                     /gene="fabG"
                     /locus_tag="ACZPAX_08345"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005921014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HIR1113945.1"
                     /translation="MPQTENLRRAALITGGAKGIGRAICRALAENGMNIAVNYAGSAA
                     AAEETAAACRALGVQAITLQADVRSPEACQKLVEDTAAAFGRIDVLVNNAGITADKLL
                     LRMTEADFDNVLATNLKGAFFCTKAACRFMMHQRYGRIISVSSVVGLHGNAGQANYAA
                     SKAGLIGLTKSTAKEYAARNITANAVAPGFIATDMTAVLSEPARAAAEAAIPAGKIGH
                     AEDVAAAVAFLAGEQAGYITGQVLCVDGGMGM"
     gene            complement(1272..2210)
                     /gene="fabD"
                     /locus_tag="ACZPAX_08350"
     CDS             complement(1272..2210)
                     /gene="fabD"
                     /locus_tag="ACZPAX_08350"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015537416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HIR1113946.1"
                     /translation="MKLAFLYAGQGSQHVGMGQDLYETFPAFRAAFDEADAAVDFDLK
                     EVCFTDAAGVLNETQYTQPCMTAFAAGLTAVLREAGVTPDYAAGLSLGEYSALTCAGV
                     FDVRTAVALTAFRGKAMAAAAAGRTCGMTAVLGLDEAPLQACCNAAADKGVVQICNYN
                     CPGQLVIGGDKAAVDEAAALALAAGARRCLPLNVSGPFHTSLMHPAGDALREKFKATH
                     FAPMQFPVLFNCLGHEMEETDTIAALLEKQVQSGVRWEATVRRLAELGVDTAIEIGPG
                     RALTGFVKKTVGSAISCYPVETATQLQQLLRTLKGA"
     gene            complement(2207..>2980)
                     /locus_tag="ACZPAX_08355"
     CDS             complement(2207..>2980)
                     /locus_tag="ACZPAX_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006574831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF561 domain-containing protein"
                     /protein_id="HIR1113947.1"
                     /translation="CRALTDKPFGVNIMLMHPQADEFAKIVVEEGVKIVTTGAGNPGK
                     YMAAWKAAGITVIPVVAAAILAKHLEPLGIDAVIAEGTESGGHVGEMTTMALVPQVVD
                     AVHVPVIAAGGIADGRGLAAALALGACGVQVGTCLLASTECPIHENYKAALLKARDSD
                     TIVTGRISGTPVRVLKNRMSREYVRQEKAGADKMALERFTLGSLRKAVFDGDTESGSL
                     MAGQAAGLLHEVRPVEEIFASMVNDAQNCIGGLHKEITL"
BASE COUNT          556 a          956 c          864 g          604 t
ORIGIN      
        1 cgcctgcaca ggcggtggtg gggcagctgc acatgccctg caagccaaaa cggatggcaa
       61 tctgccccgc cgccatattg ccaatggcca tggggacaaa atagggactg actttttcaa
      121 agccacgctg ctggccgcgg gtgtgttctt cctcgatcgt gggcaggccg ccgatgccgc
      181 tggaaaggat aacaccgaac cgtgcgcggt cgatttcttc cagtgcaaga ccgctgtccc
      241 gaaccgcttc ctcagcggcg gccacggcga actgggtaaa gcgcgccatt ttgcgggctt
      301 cacgtttgtc gatacgttcg gcggggtcaa aatccttgac ctctgcggcc agctttacct
      361 taaaatcggt cgcatcaaaa gcggtgatcg gcccaacggc gcagcggcca gccttaacag
      421 cggcccagct ttcagccacg gtattgccgg taggatttac cgtgccaagg ccggtgataa
      481 cgacccggcg tttctttttt gtgtccattg cacacctcac attcccattc cgccgtcaac
      541 gcaaaggacc tggccggtaa tgtatccggc ctgctcccct gccagaaaag ccaccgccgc
      601 tgcaacatcc tcggcatggc cgattttgcc tgccgggatg gctgcttccg ctgcggcacg
      661 ggctggttct gataacacgg cggtcatatc cgttgcaata aagccgggag ccacggcgtt
      721 ggcggtgata ttgcgggcag catactcttt ggcagtgctt ttcgtcaggc cgatcagtcc
      781 cgccttgctg gccgcgtagt tggcctgacc cgcgttgccg tgcagaccga caacgctgct
      841 gacgctgatg atgcgcccat atcgctggtg catcataaag cggcaggccg ctttggtgca
      901 gaaaaacgcc cctttcaggt tggtggcaag cacgttgtca aaatcggctt cggtcatgcg
      961 cagaagcaat ttgtcggcgg taatgccggc gttgttgaca agcacatcga tccgcccaaa
     1021 agcggccgcg gtgtcttcta ccagtttctg acatgcttcg gggctgcgca cgtccgcctg
     1081 cagtgtgata gcctgcacac caagggcgcg gcaggctgcg gccgtttcct cggcagcggc
     1141 agcgctgccg gcatagttga cggcaatgtt catgccgttt tcggccaaag cccggcaaat
     1201 ggcacggcca atgcctttgg ccccgcccgt aatcagggcg gcacggcgca agttttcggt
     1261 ttgcggcatg gtcaggctcc tttcagggtg cgcagcagct gttgcagctg cgtggccgtt
     1321 tccaccgggt agcacgagat ggcacttccc acagtctttt tgacaaaccc ggtcaacgcc
     1381 ctgccggggc cgatctcaat ggcagtgtcc acgcccaatt ctgccagacg gcggacggtc
     1441 gcttcccaac gcacgccgga ctgcacctgt ttttccagca gcgcggcaat ggtgtcggtt
     1501 tcctccatct cgtgtcccaa gcagttgaac agcaccggga actgcatcgg cgcaaagtgg
     1561 gtagccttaa atttctcccg cagtgcatcc ccggccgggt gcatcaatga ggtatggaaa
     1621 gggccgctga cattcagcgg caggcagcgc cgtgccccgg cagcaagcgc cagcgcggca
     1681 gcttcgtcca cggcggcttt gtcaccgcca atgaccagct ggccggggca gttgtagttg
     1741 cagatctgca caacgccttt gtcggcagct gcattgcagc atgcctggag gggagcttcg
     1801 tccagcccta aaacggctgt catcccgcag gtgcgcccag ccgcagccgc cgccattgcc
     1861 ttaccgcgga aagcggttaa agcgactgcg gtccgcacat cgaacacccc cgcgcaggtc
     1921 agggcggaat actcccccag tgacagcccc gccgcataat ccggtgtgac acctgcctcc
     1981 cgcagtacgg cagtaaggcc agcggcaaac gccgtcatgc agggctgggt gtattgggtc
     2041 tcgttcaata cgcctgctgc gtcggtaaag cagacttctt tcaaatcaaa atccaccgcc
     2101 gcgtcggctt cgtcaaaagc ggcgcggaaa gccgggaaag tctcgtaaag atcctggccc
     2161 atgccgacgt gctggctgcc ctgcccggcg tataaaaatg ctagtttcat aaggtgatct
     2221 ccttgtgcag cccaccaatg cagttttggg catcgtttac catcgaggca aagatttctt
     2281 ccaccgggcg gacttcgtgc agcagccctg cggcctgtcc ggccatcagg ctgccactct
     2341 cggtgtcgcc gtcaaacaca gccttgcgca gactgcctaa agtaaagcgt tccagcgcca
     2401 ttttgtcagc cccggccttt tcctggcgga cgtactcgcg gctcatgcgg tttttcagta
     2461 cgcgcacggg ggtcccgctg atgcgcccgg tcacgatggt atcgctgtca cgggccttca
     2521 acagggccgc tttatagttt tcgtggatcg ggcactcagt gcttgccagc agacaggtac
     2581 cgacctgcac accgcacgcc cccaatgcca gcgctgcagc caacccgcgc ccatcggcga
     2641 tgccgcccgc agcaatgaca ggcacatgga cggcatcgac gacctgcggg acaagtgcca
     2701 tcgttgtcat ctcacccaca tgaccgccgc tctcggtgcc ctcggcaata acggcgtcaa
     2761 tgccgagcgg ttccagatgt ttggccaaaa tggctgccgc caccacgggg atgaccgtga
     2821 tgccggccgc tttccacgcg gccatgtact ttcccggatt tcccgccccg gtggtgacga
     2881 tctttacgcc ctcttccacc acgattttgg caaattcatc cgcctgcggg tgcatcagca
     2941 tgatgttcac gccaaagggc ttatcggtca gggcgcggca
//
LOCUS       DBMDSQ010000243         2971 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_7420_length_2971_cov_2.017770, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000243 DBMDSQ010000000
VERSION     DBMDSQ010000243.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 2971)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2971)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2971
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7420_length_2971_cov_2.017770"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1416)
                     /locus_tag="ACZPAX_08360"
     CDS             complement(<1..1416)
                     /locus_tag="ACZPAX_08360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113948.1"
                     /translation="MFFLITIFLTALVSALFGLLPGKLQRRSVARQDIVWAVGLWLAT
                     WLFAVFAYHRPGLAIGFNVFRLVAVTALNGWTGFVTAGLRSFGRKGLKAVVPLLLVTA
                     LGLEVFVGNVAYFNTHSYEPFQLVDYLDENINVGRGLGTISLDEGRTYLRFLNIDRPI
                     YNLRFDGLVNDDTDLLHADSAVTFTIEGTDAANTQLTRFGSWQVGLQAPRTHTISLDL
                     TGNVSMMTLTTAGYSSTYAQFPVALTFSGITANAPRALNFSVLRFIAVFLALLAVYGL
                     RPASGLWHRRWLAGNVCDRAAAAVLGLVLAAFVVVVPFWEPGNTGLATKDYNTAFWDG
                     KSTVSFVYQQYGALAHSLLNGRLDLEADPPAELLALENPYDAGARDAAQINDIHWDHA
                     FYNGRYYVYFGIVPCLLFQLPFEALTGIQNLAYAPCMVVLGLLLLAACFGVVGQTVRR
                     WFPQTSAAAYLLLLPRKWHGGR"
     gene            complement(1476..2528)
                     /locus_tag="ACZPAX_08365"
     CDS             complement(1476..2528)
                     /locus_tag="ACZPAX_08365"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR1113949.1"
                     /translation="MNPIVINGTVLCDNITGIPRYVYETVVRLDKLIEGTGLDVRIAY
                     RDDGRPIHLPELKNIQLVPLKAVKYFYNLAVLPAYLRRTHAFYVGLASDMLLTKRSVT
                     VLHDIRPLVMDTDKGFFRFKFWVHCLSTKWFAQRVFTVSDNQRHLISEKLGIPLDKIG
                     ITYNGWEHMKGVTPDESIFEKMPGVQKGEYYYALGSLAKHKNFKWIREVARRNPDKTF
                     VVAGGKDLRAFGDDNEAKDTHNVFYPGYVSDAENAALMQHCKLFLHPAVFEGFGIPPL
                     EALALGAPIALANATCLPELYGDTARYFDPYDYDVDLDALAAQPVAAPDAVLQKYSWD
                     KTADFWLHEIEKYAKQ"
     gene            complement(2525..>2971)
                     /locus_tag="ACZPAX_08370"
     CDS             complement(2525..>2971)
                     /locus_tag="ACZPAX_08370"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR1113950.1"
                     /translation="ELDKLKAMAGPTVEFLGGGLSDEEVRSYYLRAKAFLFPGEEDFG
                     ITPVEAQSAGTPVLAYGRGGACESVVPGKTGYWFKEQTVESLADCIERFERDGVAYSK
                     EEIREHSRSFSEERFERELKEYCERRMADWQQELLDCSHWEKEELD"
BASE COUNT          595 a          893 c          930 g          553 t
ORIGIN      
        1 ccgtccgccg tgccacttcc gaggcaggag cagcaggtac gcggcggcgg aggtctgcgg
       61 gaaccaccgg cgcacggtct gccccaccac gccgaagcag gcggcgagga gcagcagccc
      121 cagaacgacc atgcagggcg cataggccag attctggatg ccggtcagcg cctcaaaggg
      181 cagctggaac aacaggcacg gcacaatgcc gaaatagacg taatagcgcc cattgtagaa
      241 ggcgtgatcc cagtggatat cgttgatctg cgccgcatcc cgtgccccgg cgtcgtaggg
      301 gttctccaac gccagcagct cggcgggcgg gtcggcctcc aggtccaggc ggccattcag
      361 caggctgtgg gccagtgcgc cgtactgctg gtagacgaag ctgacggtgc tcttaccgtc
      421 ccagaatgcg gtgttgtaat cttttgtagc aaggccggtg ttgcccggct cccagaaggg
      481 cacgaccacc acaaaggcgg ccagaaccag gcccagcacg gcggctgcgg cgcggtcgca
      541 tacattgcca gccagccagc ggcggtgcca taacccgctg gccgggcgca ggccatagac
      601 agccagcaac gccagaaata ccgcgataaa acgcagaaca gagaaattca gcgcccgcgg
      661 ggcgttcgcc gtgatgccgc tgaacgtcag cgccaccgga aactgcgcgt aggtggagct
      721 gtacccggcg gtggtcagcg tcatcatgct gacattgccg gtcagatcca ggctgatggt
      781 gtgggtgcgc ggggcctgca ggccgacctg ccagctgcca aagcgggtca gctgggtgtt
      841 ggccgcgtca gtgccctcga tggtgaaagt gacggcgctg tctgcgtgga gcaggtcggt
      901 atcgtcattg acgaggccgt caaaccgcag gttgtagatg gggcggtcga tgttcagaaa
      961 ccgcagatag gtccggccct cgtccagcga aatcgtgccc agcccgcgcc cgacgttgat
     1021 gttctcgtcg aggtagtcaa cgagctggaa cggctcatag ctgtgggtgt tgaagtaggc
     1081 aacgttgccg acgaatactt ccaaccccag cgccgtgacg agcagcagcg gaacaacggc
     1141 tttcagcccc ttgcggccaa agctgcgcag cccggcggtg acgaaaccgg tccagccgtt
     1201 cagcgctgtg acggccacga gacggaacac attgaagccg atggccagcc cggggcggtg
     1261 gtaggcaaag accgcgaaca gccaggtggc caaccatagg ccgacggccc agacgatgtc
     1321 ctgccttgca acgctgcggc gctgaagctt tccgggcaaa agcccgaaca gcgcagagac
     1381 cagcgccgtg aggaagatcg ttatcaggaa aaacatgggg cgcagtcggc tcctttcagt
     1441 gtgaataagg cttccccctt gggggaagga ttttatcact gtttcgcgta cttttcaatc
     1501 tcatgcagcc agaaatcagc ggttttgtcc cagctgtatt tctgcaggac cgcatcgggc
     1561 gcggctacgg gctgggcggc cagcgcgtcc aggtctacat catagtcgta ggggtcgaaa
     1621 taccgcgctg tatcgccgta cagttccggc aggcaggtgg cgttggcaag tgcaatgggc
     1681 gcgcccaagg ccagcgcttc cagcggcggg atgccgaacc cttcaaacac ggccgggtgc
     1741 aaaaatagct tgcagtgctg catcagcgcg gcgttctcgg cgtccgagac atagcccggg
     1801 tagaatacgt tgtgtgtgtc cttggcttcg ttatcatcgc cgaaggcccg aaggtccttg
     1861 ccgcctgcca ccacaaacgt cttgtcaggg ttgcggcggg cgacctcgcg gatccatttg
     1921 aaattcttgt gcttggcaag gctgccgaga gcgtagtagt actccccttt ctggacgccg
     1981 ggcatctttt caaaaatgct ctcgtcgggg gtcacaccct tcatatgctc ccagccgttg
     2041 taggtgatgc cgattttatc cagcgggatg ccgagctttt cactgatgag gtggcgctgg
     2101 ttgtcgctga ctgtgaacac ccgctgggca aaccacttcg tggacagaca gtgtacccag
     2161 aacttgaagc ggaaaaagcc cttgtcggtg tccatgacca gcgggcggat atcgtgcagc
     2221 acggtgacgc tgcgcttggt cagcagcata tcgctggcca ggcctacata aaacgcatgg
     2281 gtgcggcgca ggtaggcggg cagcacagcc aaattgtaaa aatatttgac cgctttcagc
     2341 ggcaccagct ggatgttttt cagctccggc aggtgaatgg ggcggccgtc gtcgcggtag
     2401 gcaatccgca catccaggcc ggtgccctca atgagtttat ccagccggac gacggtctca
     2461 tagacatagc gcgggatgcc ggtgatatta tcacacagta ccgtgccgtt gatgacgatg
     2521 ggattcaatc cagttcctcc ttctcccagt gggagcagtc gagcaattcc tgctgccagt
     2581 cggccatgcg gcgctcgcag tattctttca gctcacgctc aaagcgttcc tcgctgaaac
     2641 tgcggctgtg ctcgcgaatt tcttctttag aataggcaac gccgtcgcgc tcaaaccgct
     2701 cgatgcagtc ggccaggctt tcgacggtct gctctttgaa ccagtagccg gtcttgccgg
     2761 gcacaacact ctcgcacgcg ccgccgcggc cataggccag caccggcgta cccgcgctct
     2821 gggcttccac cggcgtgatg ccgaagtcct cttcacccgg gaaaaggaac gccttggccc
     2881 gcaggtagta gctgcgcact tcctcgtcgg aaaggccgcc gcccagaaat tccacggtcg
     2941 gcccagccat agccttgagc ttatccagtt c
//
LOCUS       DBMDSQ010000244         2967 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_7430_length_2967_cov_1.687020, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000244 DBMDSQ010000000
VERSION     DBMDSQ010000244.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 2967)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2967)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2967
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7430_length_2967_cov_1.687020"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..852
                     /locus_tag="ACZPAX_08375"
     CDS             <1..852
                     /locus_tag="ACZPAX_08375"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009323818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HIR1113951.1"
                     /translation="EHDCPANKRVAPAVRLATPRVIIPVFPGTNCEYDTARAFRRAGG
                     DPHILVLKNLSPADVAESCEALVKELDQAQILMLPGGFSGGDEPDGSAKFIASFFRNP
                     AVADAVNRLLNQRDGLALGICNGFQALIKLGLVPYGEIRPITADDPTLTFNTIHRHQS
                     MLVRTRVASNKSPWLSRCDINDEHLIAISHGEGRFVCNETLLNQLIDNGQIATQYVDL
                     TCRPTMDMRYNPNGSVLAIEGITSPDGRVFGKMGHSERSGEQLYKNVTGDKYQPIFEG
                     GVNYFKL"
     gene            873..>1562
                     /locus_tag="ACZPAX_08380"
     CDS             873..>1562
                     /locus_tag="ACZPAX_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015519861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HIR1113952.1"
                     /translation="MAQHDRYISPFSTRYASDEMQYIFSDDNKFRTWRKLWIALAKAE
                     QKQGLAITDEQLAELEAHKDDINYEDAIAREKLVRHDVMSHVYAYGLQCPKAKGIIHL
                     GATSCYVGDNTDVIVMREALQLVRKKIIGVLANLAKFADEYKSMPCLAYTHCQPAQLT
                     TVGKRATLWMNELYMDLEAIDYQISQLALRGVKGTTGTQASFMELFNGDSAKIKAVET
                     DVCAQMGFTKVV"
     assembly_gap    1563..1572
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1573..2289
                     /locus_tag="ACZPAX_08385"
     CDS             <1573..2289
                     /locus_tag="ACZPAX_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015519861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HIR1113953.1"
                     /translation="VDYNVLSAVAGLGQSAMKMATDIRLLANFKEMEEPFEKNQIGSS
                     AMPYKRNPMRCERICALSRYLMVDVLNPAMTAGTQWFERTLDDSANKRIAMAEGFLAA
                     DAILNILLNVSDGLVVYPKVVRARVMAELPFMASENIMMKAVKKGGDRQELHERLREH
                     AVAAAAVVKQEGKPNDMIARVEADPAFGLTREEIEAELSPEAFTGRSSEQVTEFLRDV
                     IQPVLDANKEDLGQHVELSV"
     gene            complement(2413..>2967)
                     /locus_tag="ACZPAX_08390"
     CDS             complement(2413..>2967)
                     /locus_tag="ACZPAX_08390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113954.1"
                     /translation="DQSRDTNFAVQTRFWLLTDDEIRGKELEPDHVFYVDHRWTTAYR
                     LGDEAAQQAAAYLTVQAPELNFASVYGAKGNTVEIDLCYTEQDGMTPANYVDDLPLDA
                     AFDKALLQKVPSRLCAQVLCDGKPTEADMQTVLHTLKQVLESNDMPITYYDITLVPTG
                     DYADHSAFMADVLESGLTSADTIA"
BASE COUNT          610 a          902 c          858 g          587 t
ORIGIN      
        1 gagcatgact gccctgccaa caagcgtgtg gcccccgctg tgcgcctggc gacgccgcgg
       61 gtcatcatcc cggtgttccc cggcacgaac tgcgaatacg acacggcccg cgccttccgc
      121 cgtgccggcg gtgatccgca cattctggtt ttgaagaacc tctcccccgc tgacgtggcc
      181 gagagctgcg aggcgctggt gaaggagctt gaccaggcgc agattttgat gctgccgggc
      241 ggtttctccg gcggcgacga gccggacggc tccgcaaagt tcattgcttc cttcttccgc
      301 aacccggcgg tggccgacgc cgtcaaccgt ctgctcaacc agcgtgacgg cctggccttg
      361 ggcatctgca acggtttcca ggcgctcatc aagctgggcc tggtgcccta cggcgagatc
      421 cgccccatca cggcggacga cccgacgctg accttcaaca caattcaccg ccaccagtcc
      481 atgctggtcc gcacccgcgt tgccagcaac aagagcccct ggctgagccg ctgcgatatc
      541 aacgacgagc atctgatcgc catcagccac ggcgagggcc gctttgtctg caacgagacg
      601 ctgctgaacc agctgatcga caacggccag attgcaacgc agtatgtgga cctgacctgc
      661 cgtccgacaa tggatatgcg ctacaatccc aacggttctg tgctggccat tgagggcatc
      721 accagcccgg acggccgtgt ctttggcaag atgggccaca gtgagcgcag cggcgagcag
      781 ttgtacaaga acgtcaccgg cgacaaatac cagccaatct ttgagggcgg tgtgaattac
      841 tttaagctgt aagaaagtag aggacttttc ccatggcaca acatgaccgt tatatttccc
      901 ctttctccac ccgctatgct tcggacgaga tgcagtatat cttctcagac gacaacaagt
      961 tccgcacctg gcgcaagctg tggatcgcgc ttgccaaggc tgaacagaag cagggcctgg
     1021 caatcaccga tgagcagctt gctgagctgg aagcccacaa ggacgacatc aactacgagg
     1081 atgccatcgc ccgcgagaag ctggtgcgcc acgacgttat gagccatgtc tacgcctatg
     1141 gcctgcagtg ccccaaagca aagggcatca tccatctggg cgcgaccagc tgctatgtcg
     1201 gcgacaacac cgatgttatc gtcatgcgcg aggctttgca gctggtgcgc aagaagatca
     1261 tcggcgtgct ggcaaaccta gctaagttcg ccgacgagta caagtctatg ccctgcctgg
     1321 cctacaccca ctgccagccc gcgcagctga ccaccgtcgg caagcgtgcc acactctgga
     1381 tgaacgagct gtacatggat ctggaagcca tcgactacca gatttcccag ctggcgctgc
     1441 gcggcgtcaa gggcaccacc ggcacccagg ccagctttat ggagctgttc aacggcgact
     1501 ccgccaagat caaggccgtc gagaccgacg tctgcgccca gatgggcttt accaaggtcg
     1561 tgnnnnnnnn nngtggacta caacgtcctg tccgccgtgg cgggtctggg ccagtctgcc
     1621 atgaagatgg cgaccgacat ccgcctgctg gcaaacttca aggaaatgga ggagccgttc
     1681 gagaagaatc agattggttc ctcggccatg ccctacaagc gcaaccccat gcgctgtgag
     1741 cgcatctgcg ccttaagccg ctacctgatg gtggatgtgc tgaaccccgc catgaccgct
     1801 ggtacccagt ggtttgagcg cacgctggac gactctgcca acaagcgcat tgcgatggcg
     1861 gagggtttcc tggctgctga cgcaattctg aatattttgc tgaatgtctc tgatggtctc
     1921 gtcgtctacc cgaaggttgt ccgcgcccgt gtcatggccg agctgccctt catggccagc
     1981 gagaacatca tgatgaaggc tgtcaagaag ggcggcgatc gtcaggagct gcacgagcgc
     2041 ctgcgcgagc acgccgtggc tgctgctgcc gtcgtcaagc aggagggcaa gcccaacgac
     2101 atgattgccc gcgtcgaggc tgaccctgcc ttcggcctga cccgcgagga aattgaggct
     2161 gagttgtccc ccgaggcctt caccggccgc agcagcgaac aggtcaccga gttcctgcgc
     2221 gacgtcatcc agcctgtgct ggacgccaat aaagaggact tgggccagca tgtagagctg
     2281 agcgtctgat ttattgcatc gtaaattgta ggggcgaatg tttgcatccg cccgcaggtc
     2341 gatgacaagc atcgacccct acagaacagc aaaatacccg tattgtctta ctaaaggcaa
     2401 tacgggtatt ttttatgcaa ttgtatcggc ggacgtcagg ccggattcca acacatccgc
     2461 cataaacgcg ctgtggtcag cgtaatcgcc ggtgggcaca agggtgatgt cgtaataggt
     2521 gatcggcatg tcattgcttt ccagcacctg cttgagggtg tgcaggacgg tttgcatgtc
     2581 cgcctcggta ggcttgccat cgcacagcac ttgtgcgcac aggcggctgg gcaccttttg
     2641 cagcagggct ttgtcaaagg cagcatccag cggcagatca tccacatagt tagccggggt
     2701 catgccgtcc tgctcggtat agcaaaggtc gatctctacc gtgttgccct ttgcgccgta
     2761 gacagatgca aagtttaatt ccggggcctg cacggtcaga tacgcggctg cctgctgggc
     2821 ggcttcatcc cccaggcggt aggcggtggt ccagcggtgg tctacataga acacatggtc
     2881 tggctcaagc tccttacccc ggatttcatc atcagtcagc agccagaacc gcgtctgcac
     2941 cgcaaagttt gtatcacggc tctggtc
//
LOCUS       DBMDSQ010000245         2964 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_7447_length_2964_cov_1.730353, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000245 DBMDSQ010000000
VERSION     DBMDSQ010000245.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 2964)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2964)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2964
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7447_length_2964_cov_1.730353"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(490..981)
                     /locus_tag="ACZPAX_08395"
     CDS             complement(490..981)
                     /locus_tag="ACZPAX_08395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HIR1113955.1"
                     /translation="MPGPQLPHNCIFTTKMPIGFVPMGVLLYLSELKQFAHRDIQHPC
                     NAKDHIQRDRTVTVLDAGHMGAAYVDLIRQLNLRQPRRLAVVGVVIPYSADGWPIVVN
                     TDYVLKSAPSVNSDDVVWVSAGDKLYGHYGDGDWVFVADYDFNVYGWIPEPYQEFDGS
                     TVQ"
     gene            complement(1027..2124)
                     /locus_tag="ACZPAX_08400"
     CDS             complement(1027..2124)
                     /locus_tag="ACZPAX_08400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113956.1"
                     /translation="MNTENLNGILAQYIQYCKTRTAAEEGTVWRAADCFGVNWDIEDS
                     DFPGMFADAMQQAQLALDNPALQPIGGVQTLMLRETEVELVRECFRWLFKEDDGDIAK
                     RRGRAELFADQINGRFRRVFPRMPKYTMNTANAVFFLNLREPHDNYFYNAAEARAWAE
                     YFDYEADFGGGTALDLPRYYTMCNDLLAELESYPELIALHKAYVAQELGGINDQLHLL
                     VYDILHTAYTQQFYPCGYARSATTKERAKAAKEKATRAELCIRIGECEQKLQEMQANP
                     AKLPDLTDCKISHKMFGAGTVRPAEGQFMVIDFNGTLKKFSYTASMNSGYLTAEDPAV
                     EAELQAYQDYNKEKDRLEKELKNMKAELVKL"
     gene            complement(2311..2949)
                     /locus_tag="ACZPAX_08405"
     CDS             complement(2311..2949)
                     /locus_tag="ACZPAX_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008402004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HIR1113957.1"
                     /translation="MAMNLVKLPTGKPNLQLRVSKGHYATSHSHINYYIDVTLTKFRL
                     SEARAAAAELIRAYKATTIVDTILCLDGTEVIGACMASELTKSGYVNMNAHQTIYVVT
                     PEHTTGSQLLFRENTAPMISGKHILVLAASVTTGYTVQGAIEAIRYYGGEAVGIAAIF
                     ASVNECAGYPVASIFNTHDLPDYETHDSHSCPWCKQGKKIDALVNSFGYSSL"
BASE COUNT          606 a          823 c          841 g          694 t
ORIGIN      
        1 tcgactccca tcagccgctc caaaaagaga cccctgcgtc gggacacgac acaggggtct
       61 ctttttggat tcagggatga ggagtcgaac aggacgacgg caccgcagtg ccggggcgaa
      121 cagtcctgcg gactgttcgc cagcccgcgg gaggcggaca tcaaccgctc tggaaaactc
      181 cctatgtcat attccggcat ggggagtttt gcttattatg ccagcaaaaa attctgaaat
      241 ctgcttgaca gtatcttcac ctgccggggt ggcggttctt gctttttacg accaacctta
      301 gcgtaacggt cttgaaaccg atatggaaag tcaatgtaaa acgcattgcc tcaccccctt
      361 tcgggaagtg tggctgaccg cctgccgttt tgtgcagcgc cttaccagcg aagcggtatg
      421 tttagaatat catatttcga cacaccacga aagttattca tgcaaaaagt tcccgacgct
      481 ttttgcattt tattgtacgg tgctcccgtc aaattcctga taaggttcgg gtatccaacc
      541 gtatacgtta aagtcgtagt ctgcgacaaa gacccagtca ccgtcgccat aatgaccgta
      601 cagtttatca cccgcgctga cccacactac gtcatcacta ttgacgctgg gcgcggattt
      661 taacacatag tccgtattca cgacaatggg ccatccatcc gcactatacg gaatcacaac
      721 tcctactacc gcaaggcggc ggggctgtcg caaattcagc tggcggataa gatcgacata
      781 agccgcaccc atatgtcccg catcgagaac agtgactgtg cggtctcgct ggatgtggtc
      841 tttcgcattg cacgggtgct ggatgtccct gtgtgcaaac tgtttgagtt ccgataaata
      901 cagcaaaacg cccatcggca caaaaccgat gggcattttc gttgtaaata tgcagttatg
      961 gggaagttgc gggccgggca tgcccggccc ctacggggcg gcgacataga cgtggtattg
     1021 tctgccttac agcttcacca actccgcctt catgtttttc agttcctttt ccaggcggtc
     1081 tttctctttg ttgtagtcct ggtaggcttg cagttcagcc tcgacagcgg ggtcctcggc
     1141 ggtcaggtag ccgctgttca tcgaggccgt gtagctgaac tttttcagcg tgccgttaaa
     1201 gtcgatgacc atgaactggc cttcggcggg ccgaaccgta cctgcgccga acatcttatg
     1261 gctgatctta cagtcagtca ggtcgggcag tttggcgggg ttggcttgca tctcctgcaa
     1321 tttctgttcg cattcgccga tgcggatgca cagctcggcg cgggttgctt tctccttagc
     1381 ggccttggcg cgttcctttg tagtggcgct gcgggcatag ccgcacgggt agaactgctg
     1441 ggtgtaggcc gtgtgcagga tatcgtacac caacagatgt agctggtcat tgatgccgcc
     1501 aagttcctgc gccacatagg ccttgtgcag ggcaataagt tccgggtagc tttccagctc
     1561 ggccaacagg tcgttgcaca tagtatagta acgtggcagg tccagtgccg tgccgccgcc
     1621 gaaatcagcc tcgtagtcaa aatattctgc ccaggccctg gcctcggccg cattataaaa
     1681 atagttgtcg tgaggttccc tcaggttcag gaaaaacacc gcgttggccg tgttcatcgt
     1741 gtacttgggc atccgcggga acacgcgacg gaaccggccg ttgatctggt cggcaaacag
     1801 ctcggcgcgg ccgcggcgct tggcgatatc gccgtcatcc tctttgaaca gccagcggaa
     1861 acactcccgc accaactcta cttctgtctc ccgcagcatc agagtctgca cgccgccgat
     1921 gggctgcaag gccgggttgt ccagcgccag ctgtgcctgc tgcatggcgt ccgcaaacat
     1981 accggggaag tcgctgtcct cgatatccca gttcacgccg aagcagtcgg ccgcacgcca
     2041 gaccgttccc tcttcggcgg cggtgcgggt cttgcaatat tgaatgtact gtgccaaaat
     2101 cccattcaga ttttccgtgt tcaagtgaac tcctcctcgt aaaaatcagc cttcctatta
     2161 taaaaggttg aacaggatgc gtcaacctgt aaaaacgaca aaaaagcccc cttgggtaag
     2221 ggggcaaaat gtatagaatt gtaaggctcg gggccgggca aagtccgccc tcatccggcc
     2281 tgcggccacc ttctccttca ggggaaggct ttacaggctc gaatagccga agctgttgac
     2341 gagggcatcg atttttttgc cctgtttgca ccaggggcag ctgtgggaat cgtgcgtctc
     2401 atagtcgggc aggtcgtggg tgttgaagat gctggccacc gggtagcccg cgcactcatt
     2461 caccgaggcg aagatcgccg caatgccgac cgcctcgccg ccgtagtagc ggatggcctc
     2521 gatcgcgcct tgtacagtgt agccggtggt gacggacgcc gccagcacca ggatatgctt
     2581 gccggagatc atgggggccg tgttctcgcg gaacaacagc tggctgccgg tggtatgctc
     2641 cggcgtgaca acgtagatgg tttggtgggc gttcatgttg acgtagccgg acttggtcag
     2701 ctcactggcc atgcaggcac cgatgacttc agtgccgtcc agacagagga tggtgtccac
     2761 gatggtcgtc gccttgtagg cgcggatcaa ctctgcggcg gcggcacggg cctcagacag
     2821 gcggaacttg gtcagcgtga cgtctatgta atagttgata tggctatggc tggtagcata
     2881 gtgccccttg ctgacgcgca gctgcaggtt cggtttgccg gtgggcagtt taacaagatt
     2941 catcgccatg gtggagtcct ccct
//
LOCUS       DBMDSQ010000246         2963 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_7450_length_2963_cov_1.946890, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000246 DBMDSQ010000000
VERSION     DBMDSQ010000246.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 2963)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2963)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2963
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7450_length_2963_cov_1.946890"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..558)
                     /locus_tag="ACZPAX_08410"
     CDS             complement(<1..558)
                     /locus_tag="ACZPAX_08410"
                     /inference="COORDINATES: protein motif:HMM:NF012869.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HIR1113958.1"
                     /translation="MIFGKYINRYYLKHAPVLLLGILSLLTVDYIQLLIPELYRLVIN
                     GVNLGQVVVDGQTMAFTKEVLFQHICLPMIYIVVLMVIGRFLWRVCFFGSAVRVAADL
                     REQMFDHSRQLSQQYYQVNKVGNLMSLYTNDLDTIQECFGDGILMFFDASVLGILALV
                     KMWRMDIKLTLLALIVVAMNAVFWGM"
     gene            complement(705..1364)
                     /gene="fsa"
                     /locus_tag="ACZPAX_08415"
     CDS             complement(705..1364)
                     /gene="fsa"
                     /locus_tag="ACZPAX_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012198047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HIR1113959.1"
                     /translation="MKFYIDTANVDEIRKANDMGIIAGVTTNPSLIAKEGRDYVETLK
                     EIATIVDGPISGEVKATTTDAEGMIAEGRAIYALDPKHMVVKIPMTAEGLKAIKVLSA
                     EGIPTNCTLIFSANQALLAARAGAAYVSPFLGRLDDIGQPGIDLVETIHDMFLNYPDI
                     ETQIIAASVRNPIHVTDCALAGADIATVPYKVIEQMLHHPLTDAGIEKFKADYTKVFG
                     A"
     gene            complement(1378..2073)
                     /locus_tag="ACZPAX_08420"
     CDS             complement(1378..2073)
                     /locus_tag="ACZPAX_08420"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006574218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HIR1113960.1"
                     /translation="MLEALKQQVYEANMELPRRSLVTYTWGNVSGIDRERGLFVIKPS
                     GVEYDELTSEMLVVMDLDGNKVEGDLNPSSDTKTHLELYKAFPQLGGIVHTHSTHAVA
                     FAQARRDLPAFGTTHADYFYGPVPCTRELTPEEIDEDYEKNTGKVIIETFTERGIDPV
                     HVPGVLCASHGPFTWGKDAAQAVYHAVVLEEVAKMAILTLTIDPNAHPAPQHVLDKHF
                     MRKHGPNAYYGQK"
     gene            complement(2077..>2963)
                     /locus_tag="ACZPAX_08425"
     CDS             complement(2077..>2963)
                     /locus_tag="ACZPAX_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006574217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="L-arabinose isomerase family protein"
                     /protein_id="HIR1113961.1"
                     /translation="GWQVNTWPVGQLVDTMNAVTDAEIDALMDEYRAAYDFASEDTDF
                     IETVRYQAREEIAMKKMLDTEGCKAFSNTFQDLYGMRQLPGIASQHLMAQGYGYGGEG
                     DWKVAAMTAIIKAMAEGKTGGTAFMEDYTYNLVPGAMYSLGAHMLEVCPSVAAARPRV
                     EVHPLGIGDREPPARLVFEGHEGDAIVVSLIDMGGRLRLICQDIHCVKPILPMPNLPV
                     ARVMWQAMPDLTTGLECWITAGGAHHTTLTYDVTADQMKDWANMMGIEFVHITKDTTV
                     ESLEQQLFLNDLAWKLKG"
BASE COUNT          591 a          868 c          867 g          637 t
ORIGIN      
        1 cataccccaa aacacagcgt tcatggccac cacgatgagg gccagcagcg tgagtttgat
       61 gtccatgcgc cacattttga ccagcgccag aatgcccagc accgacgcat caaagaacat
      121 gaggatgccg tcgccgaagc actcttggat ggtgtccagg tcgttggtat acaggctcat
      181 caggttgccc actttgttga cctggtaata ctgctgggac agctggcggc tgtggtcaaa
      241 catctgctcc cgcaggtcgg ccgccacccg gaccgccgag ccgaagaaac aaacgcgcca
      301 cagaaaacgc ccgatgacca tgagcaccac aatgtagatc atgggcaggc agatgtgttg
      361 gaacagcact tctttggtga aggccatcgt ctggccgtcc accaccacct gccccaggtt
      421 tacaccgttg atgaccagcc ggtacagctc cgggatgaga agctggatat aatccaccgt
      481 cagcagcgac agaataccca aaagcagtac aggcgcgtgc ttgaggtagt agcggttgat
      541 atacttgccg aaaatcacgg cggttctccc tccttttata cacacattgc ggccagagcc
      601 gtaaatattt agtatatcgt accagcgcgg attcgtcaag cgtcgcacaa agaaaagcgc
      661 cctgtgccga acaatggcac agagcgcgga gaaggcaacg tgttttacgc accgaacacc
      721 ttggtgtagt cggccttaaa cttctcaatg ccggcatccg tcagcgggtg gtggagcatc
      781 tgctcgataa ctttgtaggg caccgtggca atgtccgccc cggccagggc gcagtccgtc
      841 acatggatgg ggttgcgcac gctggcggca atgatctgag tctcgatatc gggatagttg
      901 aggaacatat catggatcgt ctcgaccaga tcaatgcccg gctggccgat atcgtcaaga
      961 cgacccagga aggggctgac gtaagctgcc cccgcgcggg cggccagcaa tgcctggttg
     1021 gcactgaaaa tcagcgtaca gttggtgggg atgccctcgg cggacagcac cttgatggcc
     1081 tttaacccct cggcagtcat ggggattttt accaccatgt gcttcgggtc cagcgcgtag
     1141 atggcgcggc cctcggcgat catgccttcg gcgtcggtgg tggtagcctt gacctcgccg
     1201 gagatggggc cgtccacgat ggtggcaatc tccttcagcg tctcgacgta gtcgcggccc
     1261 tccttggcaa tcaggctcgg gttggtggtg acaccggcga taatgcccat gtcgttggcc
     1321 ttgcgaatct cgtccacatt ggcggtatca atgtagaatt tcataagaca aacctcctta
     1381 tttctgccca taataggcat tcggcccatg cttgcgcata aaatgcttgt ccaacacgtg
     1441 ctgcggtgcg ggatgggcgt tggggtcgat ggtcagggtc aggatcgcca tctttgccac
     1501 ttcttccagc acaaccgcgt gatagacggc ttgggccgca tccttgcccc aggtgaacgg
     1561 cccatggctg gcgcagagca cgccgggcac gtgtaccggg tcgatgccgc gctctgtaaa
     1621 cgtctcgatg atgaccttgc cggtgttctt ctcgtagtcc tcgtcgattt cctcaggcgt
     1681 cagttcccgc gtgcagggca ccgggccgta aaagtagtca gcatgggtcg tgccaaaggc
     1741 gggcaggtcg cgccgcgcct gggcaaaggc caccgcgtgg gtggaatggg tgtgtacgat
     1801 gccgcccagc tgcgggaaag ctttgtacag ttccaaatgt gttttcgtgt cgctgctggg
     1861 gttcagatcg ccctcgacct tgttgccgtc caggtccatg acaacgagca tttccgacgt
     1921 aagctcgtca tattcaacgc cggagggctt gatgacgaac aggccgcgct cccggtcaat
     1981 gcccgagaca ttcccccagg tgtaggtcac aaggctcctg cggggcagtt ccatattggc
     2041 ttcataaact tgctgcttta atgcttccag cataggttat cctttcaatt tccacgctaa
     2101 atcgttcaag aacagctgct gttccagact ttccaccgtg gtatcttttg tgatatggac
     2161 aaattcaata cccatcatgt tggcccagtc cttcatctgg tcggcggtca catcgtaggt
     2221 cagtgtcgtg tggtgtgcgc cgcccgccgt gatccagcat tccagcccgg tggtcagatc
     2281 aggcatagcc tgccacatga cgcgggccac cggcaggttg ggcatgggca gaatcggctt
     2341 gacgcagtgg atgtcctggc aaatcagccg caggcggccg cccatgtcga tgaggctcac
     2401 gacgatggcg tcaccctcgt ggccctcaaa caccaggcgg gcaggcggct cgcggtcgcc
     2461 gatgcccagg gggtgaacct ccacgcgggg cctggcggcg gccacggacg ggcagacttc
     2521 cagcatatgg gcacccaggc tgtacatggc cccgggcacg aggttgtagg tgtaatcctc
     2581 cataaaggcg gtgccgccgg tcttgccctc ggccattgcc tttattatag cagtcattgc
     2641 ggcaactttc cagtcccctt cgccgccgta gccatagcct tgggccatca gatgctggct
     2701 ggcgatgccc ggcagctggc gcataccgta caagtcctgg aaagtattgg aaaatgcctt
     2761 gcacccttcg gtgtccagca ttttcttcat ggcgatttcc tcgcgggcct ggtagcgcac
     2821 ggtctcgata aagtccgtgt cctcgctggc aaaatcgtag gcggcgcggt attcatccat
     2881 cagggcgtca atttccgcat cggtcacggc gttcatcgtg tccaccagct gtcccacggg
     2941 ccaggtgttg acctgccagc cga
//
LOCUS       DBMDSQ010000247         2962 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_7455_length_2962_cov_2.163579, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000247 DBMDSQ010000000
VERSION     DBMDSQ010000247.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 2962)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2962)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2962
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7455_length_2962_cov_2.163579"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..916)
                     /locus_tag="ACZPAX_08430"
     CDS             complement(<1..916)
                     /locus_tag="ACZPAX_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005940102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohexomutase domain-containing protein"
                     /protein_id="HIR1113962.1"
                     /translation="MYLDEYKRWLAADLEDADLKPELAKVEGNDDEIKDRFAVALKFG
                     TAGLRGVLGAGTNRMNIYVVRQATQGLANWVLTQGGTQTVAISYDSRLKSDVFAKTAA
                     GVLAANGIKVRIYDALMPVPALSFATRYYNCNAGIMVTASHNPAKYNGYKAYGPDGCQ
                     MTDDAAAIVYDEIQKTDVLTGAKYISFAEGVENGMIRFVGDDCKKALYDAIEARQVRP
                     GLCKTAGLKLVYSPLNGSGLVPVTHVLNDMGITDITIVPEQEYPNGYFTTCSYPNPEI
                     FEALKLGLELATKTGADLMLATDPDADRV"
     gene            complement(1042..1389)
                     /locus_tag="ACZPAX_08435"
     CDS             complement(1042..1389)
                     /locus_tag="ACZPAX_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015538317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113963.1"
                     /translation="MTNGECCRYIRTYSELEGLQHACTLVYCAASTPQGVLAQLRREQ
                     GGKVRSSTVLAPADSFGRVMRLLRYLCENGVGPEQWLEVLEDVHQPYQLLDTSKNAIN
                     IAEEPVFCGICRF"
     gene            1545..>2962
                     /locus_tag="ACZPAX_08440"
     CDS             1545..>2962
                     /locus_tag="ACZPAX_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015538316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HIR1113964.1"
                     /translation="MYSVTNSFGLNGLRGFAVAVEADVSGGLPAFSIVGLPDSAVRES
                     ADRVRAAIKNLGFRFPDRRITINLAPADVRKTGPVYDLPLLLALLTASGQLEEVPADA
                     AFLGELALDGSLRPVSGVLPMALAAAEHGIRALYVPTENAAEAAEACADTMTVYPAHT
                     AREVVEALKGIRPLSPAAAIPFDPEDAWRQVPDFADVMGQSLARRAMVIAAAGGHNVL
                     LTGAPGTGKSMLAKRLPGILPPLTREEAVETTKIYSIAGQLPQGRGLISARPFRSPHH
                     SASAAALAGGGAQFRPGECSLANCGVLFLDELPEFSRESLEVLRQPLEDGQITVSRAA
                     GSATYPSHFQLVAAMNPCKCGYYGHPTRACTCSPSAVRQYRSRVSGPLLDRIDLCVEM
                     DPIAFDELHTAAPSESSAELRKQVLAARAIQAKRYAAPGFKGVHCNAQLNAGQVRRIC
                     RMTPGAEQLRRTCPAFSWALQCT"
BASE COUNT          527 a          962 c          872 g          601 t
ORIGIN      
        1 ccacgcggtc ggcgtctggg tcggtagcca gcatcaggtc ggcaccggtc ttggtggcca
       61 gttccagacc cagcttcaaa gcctcgaaga tctcggggtt ggggtaggag caggtggtga
      121 agtagccgtt ggggtactcc tgctcgggca cgatggtgat atcggtgatg cccatatcat
      181 tgagcacatg ggtcacgggc acaaggccgg agccgttcag cgggctgtac accagtttca
      241 ggccggcagt cttgcacaga cccgggcgca cctgacgtgc ttcaatggca tcgtacaggg
      301 cctttttgca gtcatcgccc acaaagcgga tcatgccgtt ctccacgccc tcggcgaagg
      361 agatgtactt tgcgccggtg agcacatcgg tcttctggat ctcgtcgtag acgatggcgg
      421 cggcgtcatc ggtcatctgg cagccgtccg ggccgtaggc cttgtagccg ttgtacttgg
      481 ccgggttgtg ggaggcggtg accatgatac cggcgttgca gttatagtaa cgggtagcaa
      541 aactcaaagc gggcacaggc atcagggcat catagatgcg cactttgatg ccgttggctg
      601 ccagaacgcc tgcggcggtc ttggcaaaga catcgctctt caggcggctg tcgtagctga
      661 tcgccacggt ctgggtaccg ccctgggtca gcacccagtt tgccagaccc tgcgttgcct
      721 gacggacaac gtagatgttc atgcggttgg tgccggcacc cagcacgccg cgcagacctg
      781 cggtaccaaa cttcagcgcc acagcaaagc gatccttgat ctcatcgtca ttgccctcga
      841 cctttgccag ctccggcttc aggtctgcat cctccagatc cgctgccagc cagcgcttat
      901 attcatccag atacatcttg tacaccttac ctttgtttaa aatctttttc cccggatcga
      961 aatggtttgt ataaacctac ttatactata aactcttgct ccgcttatgt caattgcgta
     1021 aaatggccga agtttgtgcc cttaaaaccg acaaatgcca caaaaaaccg gttcttctgc
     1081 tatatttatt gcgtttttcg aagtatccag cagctggtag ggctggtgta catcctccag
     1141 cacctccagc cactgctccg gccccacgcc gttttcacac aggtagcgca gcagccgcat
     1201 cacccggcca aagctgtccg ccggggcaag caccgtgctg ctgcgcacct tgccgccctg
     1261 ctcccggcgc agctgtgcca gcaccccctg cggggtggat gcggcgcagt acaccagcgt
     1321 acaggcgtgt tgcaagccct ccagttctga ataggtacgg atatagcggc aacattcccc
     1381 atttgtcatg ttctctcgtt ctcctttctg ctgcattgac aactttcctg tgctttacta
     1441 taatgatttt aaacagaaaa aactgtcgaa gtatggcaaa tatatccatt attttccact
     1501 tttggcattt ttgacaaatc atgcgccatg cagggaggga caccatgtat tctgtgacca
     1561 acagctttgg cctgaacggg ctgcgcggct ttgccgttgc ggtggaagcc gatgtttccg
     1621 gcgggctgcc cgcgttttcc atcgtcggcc tgccggactc tgccgtgcgg gaaagcgccg
     1681 accgggtgcg cgccgccatc aaaaaccttg gcttccgctt cccagaccgg cgcatcacca
     1741 tcaaccttgc ccccgccgat gtgcgcaaaa ccggcccggt atacgacctg ccgctgcttc
     1801 tggcgctgct gaccgccagc ggacagttag aagaagtacc tgccgatgct gcctttctgg
     1861 gcgagcttgc actggacggc agtctgcgcc cggtgtccgg cgtgctgccc atggcgcttg
     1921 ccgctgccga gcacggcatc cgggcgctgt acgtcccgac agagaacgcc gccgaggcag
     1981 ccgaggcctg tgccgacacc atgaccgtct atcccgccca caccgcccgg gaggtggtgg
     2041 aggcactgaa gggcatccgc cccctctccc ctgccgccgc catccccttt gacccggagg
     2101 acgcatggcg gcaggtgccg gatttcgcgg acgtaatggg gcagtcgctg gcgcggcgcg
     2161 ccatggtcat tgcggccgca ggcgggcaca atgtactttt aacgggtgcg cccggcaccg
     2221 gcaaatccat gctggcaaag cggctgccgg gcatcctgcc gcccctgacc cgggaggagg
     2281 cggtggagac gactaagatc tactccatcg ccgggcagtt gccgcaggga cgcgggctga
     2341 tctctgcccg ccccttccgc agcccgcacc actcggccag cgctgccgcc cttgccggcg
     2401 gcggagcgca gttccgcccg ggcgagtgca gtcttgccaa ctgcggcgtg ctgtttttgg
     2461 acgagctgcc ggagttttcc cgcgagagct tagaggtgct gcgccagccg ctggaggacg
     2521 gtcagatcac cgtgagccgc gccgccggaa gcgccaccta ccccagccac ttccagctgg
     2581 tagccgccat gaacccctgc aaatgcggct actacggcca ccccacccgc gcctgcacct
     2641 gctcgcccag tgcggtgcgg cagtaccgca gccgggtgtc cggcccgctt ttggaccgca
     2701 tcgacctgtg cgtggagatg gatcccatcg cctttgacga gctgcacacc gctgccccct
     2761 ccgagagcag cgcggagctg cgcaaacagg tgctggctgc ccgcgccatt caggccaaac
     2821 gctacgctgc gccgggcttt aagggagtgc actgcaacgc ccagctgaac gccggacagg
     2881 tgcggcgcat ctgccgcatg acccccggtg ccgagcagct gcgccgcacc tgtccggcgt
     2941 tcagctgggc gttgcagtgc ac
//
LOCUS       DBMDSQ010000248         2890 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_7701_length_2890_cov_2.166726, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000248 DBMDSQ010000000
VERSION     DBMDSQ010000248.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 2890)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2890)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2890
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7701_length_2890_cov_2.166726"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(14..1456)
                     /locus_tag="ACZPAX_08445"
     CDS             complement(14..1456)
                     /locus_tag="ACZPAX_08445"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015536841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HIR1113965.1"
                     /translation="MACTTILVGKNASYDGSTMIARNDDSGSGHFTAKKFTVFQPQDY
                     PAVYKSVISGVEIPLPGDGLRMTAVPNAVEGKGLWAASGVNAANVGMTATETITSNPR
                     VLGADPLVKGGIGEEDIVYLVLPYVRTAREGARRLGSLLEQYGTYEMNGIAFQDVDEI
                     WWLETIGGHHWMARRVPDDVYVVMPNQLGLDTLDLDDALGEQKDFMCSADLRDFIKKY
                     HLDLSQDGALNPRDTFGSHDDADHVYNTPRAWYMLRYFNPNTFVWDGPDADFTPRSDD
                     LPWCMVPEKKVTPEDVKYVLSSHYQGTPYDPYAAAAAEKGIYRPIGVNRNDFMALLQM
                     RPDVPEDFRAVEWLAFASNAFNTMLPFYANVDTTPEYLSNTTGDVSTDNFYWASRLLA
                     AMADASYAKSVFHIERYTLSVGAKSNGFINRYDDAQRAEADPAARATLREKANEEIAA
                     MAKAETTDALNKVLFELSSGMKNAYSRSDA"
     gene            complement(1518..>2890)
                     /locus_tag="ACZPAX_08450"
     CDS             complement(1518..>2890)
                     /locus_tag="ACZPAX_08450"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015538572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HIR1113966.1"
                     /translation="FDRGPRPDRILDSLMAHHQVDIQWGNHDVVWMGAAAGSPLCIMT
                     VLKTTLAYNNLDTLEGGYGISLRQLERFAQHTYADSDVSRWLPHADPRTAAGDLTAAA
                     RMHKAVTVMMLKLEAQVIARNPDFDLQGRDFLNHIDFDAGTVDYFGTTYPLLDCDFPT
                     VDPAHPTVLTADEGKVIAGLVRSFAESERLQRHVQFLYAHGAFYKMCNGNLLYHGAVP
                     MTENGAFAAITFEGVARSGKALFDYCDRRARQGYSAPQGSPARLAGQDFLWYLWCGAK
                     SPLFGRSAMTTFERLYIADPAAHVEAKDPYYKHIADPRTAEHILREFGLEGEGSHIVN
                     GHVPVRAIEGESPIKGGGRLIIIDGGFCRAYHQRTGIAGYTLVYNSRGLILRTHQPFE
                     NTDKAIHEDEDITSKSEYIYTAPQRILVRDTDEGGRKQALIRDLTALVEAYQTGVIPQ
                     GVAQEL"
BASE COUNT          536 a          845 c          932 g          577 t
ORIGIN      
        1 atgtaggaat tatttacgca tccgaacgcg aataggcgtt cttcatgccg ctgctcagct
       61 caaacagcac cttgttcagc gcgtcggtcg tctcggcctt ggccatggcg gcaatttcct
      121 cgttggcctt ttcgcgcagg gtggcgcggg cggcggggtc agcctcggcg cgctgggcgt
      181 cgtcgtaccg gttgatgaag ccgttggact ttgcgccgac gctcagggtg tagcgctcga
      241 tgtggaacac cgactttgcg taggacgcat cggccatggc ggccagcaag cggctggccc
      301 agtagaagtt gtctgtggag acatcgccgg tcgtgttgga cagatactcc ggcgtcgtgt
      361 ccacgttggc gtagaagggc agcatcgtgt tgaaggcgtt ggacgcaaag gccagccact
      421 ccacggcgcg gaaatcctcc ggcacgtcgg ggcgcatctg caaaagcgcc ataaagtcgt
      481 tgcggttcac gccgatgggg cggtagatgc ccttctcggc ggctgcggcc gcataggggt
      541 catagggcgt gccctgataa tgggacgaca gcacgtactt tacatcctcg ggcgtgacct
      601 tcttctcggg caccatgcac cagggcagat cgtcggagcg cggcgtaaag tcggcgtcgg
      661 ggccgtccca tacgaaggtg ttgggattga aataccgcag catataccac gcgcggggcg
      721 tgttgtagac gtggtcggca tcgtcgtggc tgccgaaggt atcccgcggg ttcagcgcac
      781 cgtcctggga caggtcgagg tgatactttt tgataaagtc acgcagatca gccgagcaca
      841 tgaagtcctt ctgctcgccc aaagcgtcgt ccaggtccag cgtgtccagg cccagctggt
      901 tcggcatgac gacatagaca tcgtcgggta cgcggcgggc catccaatgg tggccgccga
      961 tggtctccag ccaccaaatc tcgtccacat cctggaaggc aatgccgttc atctcatagg
     1021 tgccgtactg ttccagcagg ctgcccaggc ggcgggcacc ctcgcgggcg gtgcgcacgt
     1081 agggcagcac aaggtagacg atatcttcct cgccgatgcc gcccttgacc agcgggtcag
     1141 cgcccagcac gcgcgggttg gaggtgatgg tctcggtcgc ggtcatgccg acgttggcgg
     1201 cgttcacacc gctggccgcc cacaggccct tgccctccac ggcgttgggc acggcggtca
     1261 tccgcaggcc gtcgccgggc agggggatct cgacgcccga aatcacggat ttgtagacag
     1321 ctgggtagtc ctgcggctgg aacacggtaa attttttcgc ggtaaaatgg ccggagccgg
     1381 aatcgtcgtt gcgggcaatc atcgtggagc cgtcatacga cgcatttttg ccgaccagaa
     1441 tggtagtgca agccatgttt gaacatctcc ttatgtatta tataacccta ttataaacct
     1501 gttttcccaa atttcaatca caattcctgt gccacgccct gggggataac gccggtttgg
     1561 taggcctcca ccagggcggt caaatcgcgg atgagcgcct gcttgcggcc gccctcgtca
     1621 gtgtcgcgca ccaggatacg ctgcggtgcg gtgtagatgt actcgctctt ggacgtgata
     1681 tcctcgtcct cgtggatggc cttgtcagtg ttttcaaacg gctggtgggt gcgaagaatc
     1741 aacccgcggg aattgtagac cagtgtgtag cccgcaatgc cggtgcgctg gtggtaggcg
     1801 cggcagaagc cgccgtcgat gatgataagc cgcccgccgc ccttgatggg gctttcgcct
     1861 tcaatagcgc ggaccggcac atggccgttg acgatatggc tgccctcgcc ttccaggcca
     1921 aattcgcgca aaatatgttc ggcggtgcgc gggtcggcaa tgtgcttgta gtaggggtcc
     1981 tttgcctcaa cgtgggcggc ggggtcggca atgtacagcc gttcaaacgt cgtcatggcg
     2041 ctgcggccaa acagcggcga ttttgccccg caccacagat accacaaaaa gtcctgccca
     2101 gccagccgcg cagggctgcc ctgcggcgca ctgtaacctt ggcgggcgcg gcggtcgcag
     2161 tagtcgaaca gcgccttgcc gctgcgggca acgccctcaa aggtgatagc cgcaaacgcg
     2221 ccgttttcgg tcatgggcac agcgccgtgg tagagcagat tgccgttgca cattttataa
     2281 aatgcgccgt gagcgtacaa aaattgcaca tggcgctgca gacgctcgct ctcggcaaag
     2341 ctgcgcacca gcccggcaat gaccttgccc tcgtcggcgg tcagcacggt ggggtgcgcc
     2401 ggatccacgg tgggaaagtc gcagtccagc aggggatagg tcgtgccaaa ataatccact
     2461 gtgcccgcgt caaagtcgat gtgatttaag aagtcgcggc cctgcaaatc aaaatccggg
     2521 ttgcgggcaa tgacctgggc ttcgagcttg agcatcatga cggtcacggc tttgtgcatc
     2581 cgcgcggcgg cggtcaaatc gcccgcagcg gtgcgcgggt ctgcgtgggg cagccagcgc
     2641 gacacatcgg aatcagcgta ggtatgctgg gcaaaacgct cgagctgccg caggctgata
     2701 ccgtagccgc cctcaagggt gtcgagattg ttgtaagcca gtgtcgtttt cagcaccgtc
     2761 atgatgcaga gcggactgcc cgccgccgcg cccatccaga cgacatcgtg gttgccccac
     2821 tggatatcca cctggtggtg ggccatcagg ctgtccagaa tgcggtcagg gcgcgggccg
     2881 cggtcgaata
//
LOCUS       DBMDSQ010000249         2875 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_7760_length_2875_cov_1.887167, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000249 DBMDSQ010000000
VERSION     DBMDSQ010000249.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 2875)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2875)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2875
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7760_length_2875_cov_1.887167"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..499
                     /locus_tag="ACZPAX_08455"
     CDS             <1..499
                     /locus_tag="ACZPAX_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005945638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HIR1113967.1"
                     /translation="AIQAVKNGEVDFAVVTTPAEPGGELKMVELMPFNEILVGGQTFA
                     PLAGRTLSLRELTAYPLIMLGSESMSRTFYRQFFLENRAELKPDIEAATTDQMLTLVR
                     SELGLAFVPEPRARGNLARGRIVQLDLREHIPPRSVCLVYDHHRPLNTAAREFRLRLL
                     NTAKK"
     gene            complement(566..1009)
                     /locus_tag="ACZPAX_08460"
     CDS             complement(566..1009)
                     /locus_tag="ACZPAX_08460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113968.1"
                     /translation="MQYTDLTKKELQREYNRMARRYTGFVREPLQLDMAEEDPLLGKS
                     ESLRAARHFGTVLNKELSDSGAARPTDAQDAVAALSTMPGCAHRTAELCRQAGVRFDA
                     VDHSTILARIIPVDAGSEQLAHAASVFAISARIAALESLQGMLRA"
     gene            complement(1176..2078)
                     /locus_tag="ACZPAX_08465"
     CDS             complement(1176..2078)
                     /locus_tag="ACZPAX_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008399765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113969.1"
                     /translation="MAKKIVHYHLPGLFEFYELYARFLPLTRAHPEYFYDWCDIASIY
                     GAPANCLWGGGRVGAGDVPPRRVLALLRAYGISARLTFSNSLLGPEHLADARCNRLCR
                     LLVEDGNVANGVIVHSDLLADYLRQTWPQLYLVSSTTKVLTDFPALRRELERPEFRYV
                     VPDFRLNKAFAQLAVLPAPLRDKVEFLCNECCRPACPDRKACYEAVSRKNLGLPGPEH
                     RCTAPDASEGYRFSRAMASPAFIGVGDIRDTYLPMGFTNFKIEGRGLGSAVVLEFLLH
                     YLTKPAYHLAVREAVYLDAELDLF"
     gene            complement(2301..2516)
                     /locus_tag="ACZPAX_08470"
     CDS             complement(2301..2516)
                     /locus_tag="ACZPAX_08470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113970.1"
                     /translation="MNKTLSISAMQYDFHTLLKVSDICGLTGEIGFHDTPNGYLISFP
                     DGDGKADKRMAEYQSRLKDLENNIWNR"
     gene            complement(2588..>2875)
                     /locus_tag="ACZPAX_08475"
     CDS             complement(2588..>2875)
                     /locus_tag="ACZPAX_08475"
                     /inference="COORDINATES: protein motif:HMM:NF019287.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113971.1"
                     /translation="DTQSHGLVMELPPYHKPKWRHILRSSLMKAWDIFWRAFTVIYAV
                     SIAFNVPCTMTVAATYRENHSAKWIVLSIGYYVAFSLAISCVFYHIGLLIW"
BASE COUNT          617 a          871 c          827 g          560 t
ORIGIN      
        1 ggccatccag gccgtgaaaa acggcgaggt agatttcgcc gtcgtgacca cccccgccga
       61 gccgggcggc gaactgaaaa tggtcgagct gatgcccttc aacgaaatcc tcgtcggcgg
      121 gcagaccttt gcgccgctgg cggggcggac actctctttg cgagagctga ccgcctaccc
      181 acttatcatg ctgggcagcg agagtatgag ccgcacgttc taccgccagt ttttcttgga
      241 aaaccgcgct gaactcaagc cggatatcga ggccgccacc accgaccaga tgttgacgct
      301 ggtgcgcagt gagttgggcc tggcctttgt gccggagccg cgggccaggg gcaacctggc
      361 ccgcgggcgc attgtgcagc ttgacctgcg ggagcacatc cccccgcggt cggtctgcct
      421 ggtttacgac caccaccgcc cactgaacac tgccgcccgt gagttccggc tgcggctgct
      481 gaatactgcc aaaaaataga atgcgcccgc cccagttcca cagggaaccg gggcgggcat
      541 attttgtatc acgtatcaca aaatcttatg cacggagcat gccttgcagg ctttccagtg
      601 cggctatgcg ggcgctaatg gcaaacacgc tggctgcgtg ggccagctgt tccgaaccag
      661 catccaccgg gatgatgcgg gccaggatcg tgctgtgatc caccgcgtcg aaccggacgc
      721 cagcctggcg gcacaactcg gcggtgcggt gggcacagcc gggcatcgtg gacaaggctg
      781 ccacggcgtc ctgggcgtcg gtggggcgcg ctgcgccgct gtcactcaac tccttgttta
      841 gcacggtgcc aaaatgccgc gccgcacgca ggctctcgct tttgcccagc agggggtcct
      901 cctcagccat gtccagctgc aaaggttccc gcacaaagcc ggtgtagcgc cgggccatgc
      961 ggttgtattc tcgctgcaat tcctttttcg tcaagtccgt gtattgcata ttctccactc
     1021 ctcttgcatg gtatagtata cgcaaggagc ggcagtatgt aaaaagtatt ttttatatgc
     1081 ttgttatata aaaatgatat ggcagagcca ttttgtaggg aggggtcttg acccctccgc
     1141 ggggcgtcga ggacgccgcc tcctacaaca aaaatctaaa acaaatccaa ctccgcgtcc
     1201 aggtacaccg cttcacgcac ggcaaggtgg tacgcgggct tcgtcaggta gtgcagcaaa
     1261 aattccagca caaccgcgct gcccaagccc cggccctcaa ttttaaagtt cgtgaacccc
     1321 atcggcaggt aagtgtcgcg gatatccccc acgccgataa acgcggggct tgccatcgcg
     1381 cgggagaagc ggtatccctc gctcgcgtcg ggcgcggtgc agcggtgttc cgggccgggc
     1441 aggccgagat ttttgcggct gacggcctcg taacaggctt tgcggtcggg gcaggcgggg
     1501 cggcagcact cgttgcacaa aaattccacc ttgtctcgca gcggcgcggg caggacggcc
     1561 agttgtgcaa acgccttgtt caggcgaaaa tccggcacaa catagcgaaa ttccggccgt
     1621 tccagctctc ggcgcagcgc gggaaaatcc gtcagcacct tggtggtgga cgacacaaga
     1681 tacagctgcg gccaggtctg ccgcaggtag tccgccagta ggtccgaatg cacaatgacg
     1741 ccgttcgcca cgttgccgtc ctcgaccaac aggcggcaga gccggttgca ccgtgcgtcg
     1801 gccaggtgct ccggccccaa cagagagttg ctgaaagtca gccgggccga aataccgtac
     1861 gcccgcagta gggccagcac gcggcggggc ggcacatccc cggcacccac gcgtccgccg
     1921 ccccacagac agttggccgg ggcaccgtaa atcgacgcta tatcgcacca atcgtaaaaa
     1981 tattccgggt gggcgcgggt tagcggcaaa aaccgggcgt agagttcgta aaattcaaac
     2041 aagcccggca ggtgataatg tacaattttt tttgccatgg tgtgcccctt cccacaaagt
     2101 ttctacccgc attataccac cccgcacggg agcagggcaa cggcaaattt acaccgccca
     2161 agacggaagg cggcaagatg aacgaaaaca ttactctttc cggcgtgccg ccccctgcaa
     2221 cagcgcgata acacaaaaac acccccagtc gggtacatgc atcacgcgtt cccgaccggg
     2281 ggctatttca ttctcttatc ttaccgattc caaatattat tttccaaatc cttcaatcgg
     2341 ctctggtact cagccatccg cttgtcggcc ttgccgtcgc cgtctggaaa actgatgagg
     2401 taaccgttcg gcgtgtcgtg aaagccaatt tccccggtca ggccacagat atcggaaacc
     2461 ttgagcagcg tgtggaaatc atactgcatg gcgctgatgg aaagtgtctt gttcatagcc
     2521 gactcctacc ttgtaaaatc atttctctga ttataccgca gccggcgcaa aaatgcaaat
     2581 ggacttttta ccaaatcagc agcccgatgt ggtagaatac gcagctgatg gccagcgaga
     2641 acgccacata atacccgatg gacagcacga tccacttggc agagtggttc tcgcggtagg
     2701 tcgcggctac ggtcatggtg cagggcacat taaaggcgat ggacacggcg tagatgaccg
     2761 tgaacgcgcg ccagaagata tcccacgcct tcatcaaact ggaacgcagg atgtgcctcc
     2821 acttcggctt atggtagggc ggcagctcca tcacaaggcc gtggctctgg gtgtc
//
LOCUS       DBMDSQ010000250         2867 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_7787_length_2867_cov_1.574476, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000250 DBMDSQ010000000
VERSION     DBMDSQ010000250.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 2867)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2867)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2867
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7787_length_2867_cov_1.574476"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            52..2373
                     /locus_tag="ACZPAX_08480"
     CDS             52..2373
                     /locus_tag="ACZPAX_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005931613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HIR1113972.1"
                     /translation="MDPLSNKTSTTFHYAQQAQPAPAPAPQEDFELQLQPAEEPAVPV
                     YTPPITPAPTPAPAQEPMDELDALINRAVSGHAPYYAPQEVSAETTLDLPQPTEFETP
                     LTPVPQFDTPLIPVEDEPEPEPVQLPQLDMTGRVQGDTMVISPADLAAQTTAPLGGTP
                     APSVPVAPVAPAPAPAAIPTLGGSFTDTGSAVSAAWQSGRSVSEMLDELAAEPAAQPA
                     PTMTRPVTETTQDSFTVPQQGEAARVRVSTAVGNSASAPIHTPPSAAKADPNAMRLPT
                     SEEQPPEPYCYPSVNLFNPTRPDDEAGAAREMKKNADILVNTLDSFGVKTTMLDICRG
                     PSVTRYELQPQAGIKVSRITSLADDIALNLATAGVRIEAPIPGKPAVGIEVPNKIRST
                     VNIRSVFESQNYVNMRSPLTMALGKDIAGTAQVADLCKMPHLLIAGSTGSGKSVCVNS
                     IIISFLFRSGPEDVKLILIDPKVVELAEYNGIPHLLMPVVTEPRKAAGALGASVAEME
                     RRYKLFAENNVREIKAYNKLAAQTGMEHLPYIAIVIDELADLMMVAGKEVEDYICRIA
                     QKARAAGIHLIVATQRPSVDVITGLIKANIPSRIAFAVSSQIDSRTILDSGGAEKLLG
                     NGDMLFLPVGASKPVRVQGTFVTDEEIGAVLSFIKSTSSAQYDEEMIAEMERRAVAEK
                     GSKKGSDDDGDAGGALDTMFEQAVECVIDAGQASTSLLQRRCKLGYARAARIMDQMEQ
                     EKIIGPYEGAKPRAVLVTKAEWEERKLGGQYDE"
     gene            2363..2839
                     /locus_tag="ACZPAX_08485"
     CDS             2363..2839
                     /locus_tag="ACZPAX_08485"
                     /inference="COORDINATES: protein motif:HMM:NF012955.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR1113973.1"
                     /translation="MTSNRFKKAASIAVAALLLVLLCAALVIQFAGGKGPVPTWQQLR
                     ACFGVPLQTEESAPQIADGSTVVYVLDVGQGDAVLLCQDGRWCLIDTGPAEAEDALLY
                     DLDALEVETLDYLVLTHPHADHTGNARAVLQNHPECPENGNNHPDTKYTLAGIGNV"
BASE COUNT          558 a          967 c          835 g          507 t
ORIGIN      
        1 acccacgacc ctctggagcc ggtcaacatc ggcccgggcg gcaccttcgg catggacccg
       61 ctctccaaca agacaagcac cactttccat tacgcccagc aggcacagcc cgccccggcc
      121 cccgcgccgc aggaggattt tgagttgcag ttacagcctg ccgaggaacc cgctgtgccc
      181 gtctacacgc cgcccatcac gcccgcgccc accccggcac ccgcgcagga gccgatggat
      241 gagctggatg cgctgatcaa tcgcgccgtg agcggccacg cgccttatta tgctccccag
      301 gaggtcagcg ccgagacaac gctggacctg ccgcagccca ccgagtttga aactccgctg
      361 acgccggtgc cccagttcga cacgccgctg atcccggtgg aggacgaacc cgagccggag
      421 ccggtgcagc tgccgcagct ggatatgacc ggccgcgtgc agggtgacac aatggtcatt
      481 tccccggctg atttggccgc ccagactacc gccccactgg gcggtacgcc tgctccatct
      541 gttcctgttg cacctgtcgc cccggcccct gcgcccgcag ctatcccgac gctgggcggc
      601 agctttaccg ataccggcag tgcggtcagt gccgcctggc agtccggccg ctctgtctcg
      661 gagatgctgg atgagctggc cgctgagcct gccgcgcagc ctgcgcccac catgacccgc
      721 ccggttaccg agacgacgca ggacagcttc accgtgccgc agcagggtga ggccgcccgc
      781 gtgcgggttt ccaccgccgt aggcaactcg gccagtgcgc ccatccatac accgccatcc
      841 gccgctaagg cagaccccaa cgccatgcgg ctgcccacat cggaggagca gccccccgag
      901 ccgtactgct atccgtcggt caacctcttt aaccccacac gccccgacga tgaagccggt
      961 gccgcccgcg aaatgaagaa gaacgccgac atcctcgtca acacgctgga cagcttcggc
     1021 gtcaagacca cgatgctgga tatctgccgc ggtccctctg tcacccggta cgaactgcag
     1081 cctcaggctg gtatcaaggt cagccgcatc acaagcctgg ccgatgatat cgccctgaac
     1141 ctggccactg cgggtgtccg tatcgaggct cccatccccg gcaagcccgc cgtcggcatc
     1201 gaagttccca ataagattcg ttctaccgtc aatattcgtt ctgtctttga gtcgcagaac
     1261 tacgtcaaca tgcgcagccc gctgacgatg gccctgggca aggatatcgc cggtacggcc
     1321 caggtcgccg acctttgcaa gatgccccac ctgctgattg caggttccac cggcagcggt
     1381 aaatccgtct gcgtcaactc tatcatcatc agcttcctgt tccgttccgg gccggaggat
     1441 gtcaagctca ttctcattga ccccaaggtc gttgaattgg ccgagtataa cggcatcccg
     1501 catctgctga tgcctgtcgt caccgagccg cgcaaggccg ccggtgcgct gggtgccagc
     1561 gtcgccgaga tggagcgccg ctacaagctc tttgccgaaa ataatgtccg cgagatcaaa
     1621 gcctacaaca aactggccgc ccagaccggc atggaacacc tgccttatat cgccatcgtc
     1681 attgacgagt tggccgattt gatgatggtt gccggcaaag aggtcgagga ctacatctgc
     1741 cgtatcgcgc aaaaagcccg cgccgccggt atccacctga ttgttgccac ccagcgcccc
     1801 agcgttgacg tcatcaccgg cctgatcaag gccaatatcc ccagtcgtat cgcttttgct
     1861 gtttccagtc agatcgacag tcgaaccatt ctggacagcg gcggtgccga aaagctgctg
     1921 ggcaacggtg atatgctctt cctgccggtt ggtgcctcca aacccgttcg cgtgcagggt
     1981 accttcgtga ctgatgaaga gattggcgcg gtgctgagct ttatcaagtc cacttcctcc
     2041 gcccagtacg acgaagaaat gattgccgaa atggagcgcc gcgctgttgc tgaaaagggc
     2101 agcaagaagg gcagcgacga tgacggcgac gcaggcggcg cactggatac catgtttgag
     2161 caggccgtcg aatgcgtcat cgatgccggg caggccagca ccagcctgtt gcagcgccgc
     2221 tgcaagctgg gctacgcccg cgcggcccgc atcatggatc agatggagca ggaaaagatc
     2281 atcggcccct atgagggtgc caagccccgc gccgtgctgg tgaccaaggc cgagtgggag
     2341 gagcgcaagc tgggcggcca gtatgacgag taaccgcttc aaaaaggctg ccagcattgc
     2401 ggtggctgct ttgctgcttg tgctgctctg cgccgcgctc gtcatccagt ttgcgggcgg
     2461 caaaggccct gtgcccacct ggcagcagct gcgcgcttgc ttcggtgtgc cgctgcaaac
     2521 cgaggaaagt gccccgcaaa tcgccgacgg cagcactgtt gtctatgtgc tggacgtggg
     2581 gcagggcgac gccgtgctgc tctgccagga tggccgctgg tgcctgatcg acaccggccc
     2641 cgccgaagcc gaggacgcac tgctgtacga tttggatgca ctggaggtcg aaacactcga
     2701 ctaccttgtg ctgacccatc cccacgctga ccacaccggc aatgcccggg cggtgctgca
     2761 aaatcacccg gaatgcccgg aaaatgggaa taaccacccc gatacaaaat acaccctcgc
     2821 tggcattggg aacgtttgaa gtaaaggtca ttgacatggc ggcggca
//
LOCUS       DBMDSQ010000251         2859 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_7814_length_2859_cov_1.706309, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000251 DBMDSQ010000000
VERSION     DBMDSQ010000251.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 2859)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2859)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2859
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7814_length_2859_cov_1.706309"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(42..1454)
                     /locus_tag="ACZPAX_08490"
     CDS             complement(42..1454)
                     /locus_tag="ACZPAX_08490"
                     /EC_number="3.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004625482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HIR1113974.1"
                     /translation="MRTPEEQIRYAASITPSLRQLAWQQLEFYAFTHFGMNTFTNREW
                     GDGTEDPALFNPDALDADQWVAAVKSAGMKALILTCKHHDGFCLWPSAYTEHSVKNSP
                     WKDGKGDVVREVSDACRRGGIKFGVYLSPWDRHDARYGQGKPYDDYFVSQLTELATNY
                     GDIFCFWFDGACGEGKNGKKQIYDWERYYAVLRKLQPNAVLNVCGPDVRWCGNEAGHC
                     RKAEWSVVPAELRDAERTASKSQQADDGKFSRKIDSQDEDIGSRAVIRNARELVWYPS
                     EVDTSIRTGWFYHPEEDTDVRTAGELLDIYLDAVGANASLLLNIPPDTHGRIAAADCA
                     SLAELGAKIRQIFADNVTEKAEITADSAIAQHPITQAVDGMANTYWQAAYGQESDTIT
                     LKFPELQKVSCVVLGEHLPTGQRIESGEIWADGSKAAEFTVVGHKRICRFTPVDVQTL
                     TIKITASRTEPTLRLLEVYQ"
     gene            complement(1459..>2859)
                     /gene="gnpA"
                     /locus_tag="ACZPAX_08495"
     CDS             complement(1459..>2859)
                     /gene="gnpA"
                     /locus_tag="ACZPAX_08495"
                     /EC_number="2.4.1.211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015564994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050500 -
                     1,3-beta-galactosyl-N-acetylhexosamine phosphorylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,3-beta-galactosyl-N-acetylhexosamine
                     phosphorylase"
                     /protein_id="HIR1113975.1"
                     /translation="RPEFIIDQGYYNNQYRVPTKEYKDFQAFQRREVAGLMKEMTDIV
                     HAYGKEAMMFLGDHWIGCEPFMPEFQKSGVDAIVGSVGNGSTLRLISDIPGVKYTEGR
                     FLPYFFPDTFHEGGDPVREAKENWVTARRAILRKPIDRIGYGGYLKLACEFPEFLDYV
                     ESVCNEFRELYENIKGTTPYCVKTVAVLNSWGQQRAWGCHMVHHALYQKQNYSYAGVI
                     EALSGAPFDVKFISFDDIKADPKVLDGIDVLINVGDGDTAHTGGKVWEDPDVSATVKG
                     FVHRGGGLIGVGEPGGHQYQGRYLQLAAPLGVEKETGFTLNYDKYNWDEHRDHFILAD
                     CPDHDVDFGEGKKSIFALEGTEILIQRDKEVQMAAHEYGQGRGVYISGLPYSFVNNRV
                     LYRAILWAAHDEADLHKWFSTNYNVEVHAYVKNGKYCVVNNTYEPQDTTVYTGDGSSF
                     DLHLDANEIKWYSIEG"
BASE COUNT          563 a          853 c          798 g          645 t
ORIGIN      
        1 gcgtgccgca gcaacgcagc acgcctcttt tgcctgcatc cttactgata gacttccagc
       61 agccgcagag ttggttccgt gcgagaagcg gtgatcttta tggtaagcgt ctgcacatcc
      121 acgggggtaa agcggcagat acgcttatgt cccactaccg taaattcggc agccttgctt
      181 ccgtctgccc aaatctcgcc gctttctatg cgctgtcccg ttggaagatg ctcgcccagc
      241 acaacacagg aaaccttctg cagctccggg aatttcaaag taatcgtatc ggattcttgt
      301 ccgtaggcag cctgccaata ggtatttgcc ataccgtcca cagcctgtgt gatggggtgc
      361 tgcgcaatcg cagaatctgc cgtaatttcc gccttttctg ttacgttgtc ggcaaaaatc
      421 tgccggattt tagcacccag ctccgcaagg ctggcgcagt ccgccgcggc aatacgccca
      481 tgtgtatcag gcggaatgtt cagcagcagg ctggcgttcg caccgacggc atccagatag
      541 atgtccagca attcgcctgc cgtgcgcacg tcagtgtcct cctccgggtg gtaaaaccaa
      601 cctgttcgga tggaggtatc cacctcggac ggataccaga caagttctct tgcattgcgg
      661 ataaccgctc tgctgccgat gtcctcgtcc tgagagtcaa ttttacggga aaattttccg
      721 tcatccgcct gctgcgattt ggatgccgtg cgctctgcat cccgcagctc tgcgggaacc
      781 acgctccatt cagctttccg gcaatgccct gcctcgttgc cgcaccagcg cacatcgggg
      841 ccgcagacat tcaaaaccgc attcggttgc aatttacgca gcactgcata gtagcgttcc
      901 caatcgtaaa tttgcttttt gccgtttttg ccctcgccgc aggccccgtc aaaccagaag
      961 cagaaaatat caccatagtt tgttgcaagc tcggtcagct gggaaacgaa ataatcatca
     1021 tacggttttc cctgcccata gcgtgcatcg tggcgatccc acggggagag atagacgcca
     1081 aacttgatac ctccgcgccg acaggcatcc gagacttcgc gcacaacatc gcccttaccg
     1141 tctttccacg ggctgttctt gacgctatgc tccgtgtagg cgctcggcca caggcagaag
     1201 ccgtcatggt gcttacaggt caaaattagc gccttcatgc cggcactttt gactgcggca
     1261 acccactggt cggcatccag cgcatcgggg ttgaacagcg ccgggtcttc ggtgccgtcg
     1321 ccccactcgc ggttcgtgaa ggtattcatg ccgaaatggg taaaggcgta aaattcaagc
     1381 tgctgccatg ccaactggcg caggcttggc gtgatagagg ctgcatagcg gatctgttct
     1441 tcgggtgttc tcattctatt acccctcaat gctgtaccac ttgatctcgt tggcgtccag
     1501 atgcaggtca aaactgctgc cgtcgccggt gtaaaccgtg gtatcctgcg gctcgtaggt
     1561 gttgttgacc acacagtact tgccgttctt gacgtaggca tgaacttcca cgttgtagtt
     1621 cgtgctgaac cacttgtgca ggtcagcttc atcgtgggcg gcccacagaa tggcgcggta
     1681 cagcacgcgg ttgttcacga agctgtaggg cagaccgctg atatagacgc cgcggccctg
     1741 gccgtattcg tgggcggcca tctggacttc tttgtcacgc tgaatgagga tttcggtgcc
     1801 ttccagcgcg aagatactct ttttgccctc gccaaagtcc acgtcatggt cggggcagtc
     1861 ggccagaata aagtggtcgc ggtgctcgtc ccagttgtac ttgtcgtagt tcaaggtaaa
     1921 gccggtttcc ttctccacgc caagcggcgc ggccagctgc aaataacggc cctgatactg
     1981 atggccgccc ggctcgccga caccgatcag accgccgccg cgatggacaa agcccttgac
     2041 ggtagcggaa acatccggat cctcccaaac cttgccgccg gtgtgggcgg tgtcgccgtc
     2101 accgacgttg atgaggacgt caatgccgtc cagcaccttc gggtcagcct tgatgtcgtc
     2161 aaagctgatg aacttcacat caaacggcgc accggacagc gcctcgataa cgcccgcata
     2221 gctgtaattc tgcttctggt acagcgcatg gtggaccatg tggcagcccc aggcgcgctg
     2281 ctgcccccag ctgttcagaa cagccacggt cttgacgcag tagggcgtgg tacccttgat
     2341 gttctcgtac agctcgcgga actcgttgca gacgctctcg acataatcga gaaattccgg
     2401 gaactcgcag gccagcttca ggtaaccgcc gtagccgatg cggtcaatgg gcttgcgcag
     2461 gatggcgcgg cgcgcggtga cccagttttc cttggcctcg cgcacagggt cgccgccctc
     2521 gtggaaggta tcggggaaga agtagggcag gaagcggccc tcggtgtact tgacaccggg
     2581 gatgtcggaa atcaggcgca gggtgctgcc gttgccgaca ctgccgacaa tggcatccac
     2641 gccggacttc tggaattcgg gcatgaacgg ctcgcagcca atccagtggt cgcccaggaa
     2701 catcatggct tccttgccgt aggcgtggac gatgtcggtc atttccttca tcagcccggc
     2761 gacctcgcgg cgctggaacg cctgaaagtc cttgtactcc ttggtgggca cgcggtactg
     2821 gttgttgtag tagccctgat caatgataaa ctccgggcg
//
LOCUS       DBMDSQ010000252         2839 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_7875_length_2839_cov_1.391892, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000252 DBMDSQ010000000
VERSION     DBMDSQ010000252.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 2839)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2839)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2839
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7875_length_2839_cov_1.391892"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..785)
                     /locus_tag="ACZPAX_08500"
     CDS             complement(<1..785)
                     /locus_tag="ACZPAX_08500"
                     /inference="COORDINATES: protein motif:HMM:NF019310.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR1113976.1"
                     /translation="MNTTFYRLLICQIVTLFANAALRFALPLYLLRKTGSAALYGAIT
                     AAALVPMLAGTLYGGILADSCRKQTLLAVLNFITALGLLAAAICFRQIPSTAVVLGTL
                     CLLYAAEGLLQPAVQASLPLLLRDPALTQGNAAIQLADTIDELLGPLLGSLLLQSVGL
                     RTLLIFCAVCLAATALFFQTLPIPQPTRQPPAAPGQRAAVGPLLRHDPQLFGMVGVLA
                     LLNLAVVPAITVAGHKNILRCHNSPSMFGGTQKHPPYHKNYPS"
     gene            complement(892..1527)
                     /locus_tag="ACZPAX_08505"
     CDS             complement(892..1527)
                     /locus_tag="ACZPAX_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015536624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR1113977.1"
                     /translation="MSRNKYPEQTVEKILDAAACLFAQKGYAHTTLQDVIDETGLSKG
                     AVYHHFKSKEEIAAKVGDRMGAEFTATLTHIRDDDAMTGLQKLQAVFAASLLPQRQQA
                     LGRALPRLWDDPQFLSIELHDLLEKTVPVYITPMIQQGTTDGSIHTADAAALGEALFL
                     LADLWLHPLTRPTTPDQQRARCAVFRQVTQGIGLDLLTEEQSAQMVKMCEE"
     gene            1675..2514
                     /locus_tag="ACZPAX_08510"
     CDS             1675..2514
                     /locus_tag="ACZPAX_08510"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006599878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HIR1113978.1"
                     /translation="MEKIGVIDVGGGLRGIYAAGVFDWCMDNGVQFDYGIGISAGSAN
                     LASYLSSQRGRNYLFYEEYSMRKEYMGAGNMLHGGSFLNLQYIYGTLSNRECENPLDF
                     ERMTANPMEWYMIATDATTGKPKYFTRGDMRQDDYRVLMASCCIPVACKPIEIGGVPY
                     YDGALGDTIPLQKAFADGCDKVVLILTKPVGTIRADGTDRKLARVIHRKYPHAAEQLA
                     LRAQHYNEGIALAQKLAAEGKVLIIAPDDTCGVKTLTRDQKALKKLYSKGMKDAERIR
                     DFL"
     gene            complement(2515..>2839)
                     /locus_tag="ACZPAX_08515"
     CDS             complement(2515..>2839)
                     /locus_tag="ACZPAX_08515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1113979.1"
                     /translation="LGIAHFTHAVCIGQLTQQRMLISIAAYFVIGFAGQMAALVTAFF
                     VALYGYQYFSNINFSTASSIFSYDNSLPFLLLSMLAVFALIALVDALMWALTQWFMTK
                     RLNLA"
BASE COUNT          655 a          717 c          869 g          598 t
ORIGIN      
        1 taggaaggat agtttttgtg gtatggcggg tgcttttgtg tgcccccgaa catggagggt
       61 gaattatggc aacgaagtat atttttgtga ccggcgaccg tgattgccgg aacaacggcc
      121 aggttgagca gcgccagcac accgaccatg ccgaacagct gcggatcgtg gcgcagcagt
      181 gggccgacgg cagcccgctg cccgggagct gcaggcggtt ggcgtgttgg ctgtgggatg
      241 ggcagcgttt ggaaaaatag cgccgttgca gccaggcata ccgcacagaa aatcagcaat
      301 gtacgcagcc ctactgactg cagcagcaga ctgcccagca agggaccgag cagttcatca
      361 atggtgtcgg cgagctgtat ggcggcgttc ccctgcgtaa gtgccgggtc acgcagcaac
      421 aacggcagac tggcctgtac agcaggctgg agcagacctt cggcggcgta caaaaggcag
      481 agtgtgccca atacgacggc ggttgatggg atttgacgga aacagattgc ggcggccagc
      541 aggcccagcg cagtgatgaa atttaaaacg gccagcagcg tttgcttgcg gcagctgtct
      601 gccagaatgc ccccatacag tgtgcctgcc agcatcggca ccagggccgc cgccgtgatg
      661 gcgccgtaca gcgcagcgga accggtcttg cgcagcaggt acagcggcag cgcaaaccgc
      721 agtgccgcat tggcgaacag cgtaacgatt tggcagataa gtaatctata aaaggtagtg
      781 ttcatgggtg tgtctcctgt ttttgtgaaa actgttttat ataaaccgac cggcggtttg
      841 taaatgcgaa ataaaattgg cggtgcaagc cgccaaatgc aggtttttac cttattcctc
      901 gcacatcttt accatctgtg cactctgttc ctcggtcagc agatccagcc caatcccctg
      961 cgttacctgg cggaacaccg cgcaccgtgc ccgttgctgg tctggtgtgg tggggcgcgt
     1021 cagcgggtgg agccagaggt ctgccagtaa aaacagggcc tcccccaatg cggcggcgtc
     1081 cgcggtgtgg atactgccgt cggttgtccc ctgctgaatc atcggtgtta tgtaaacggg
     1141 gacagtcttt tccaacaaat cgtgcagctc aatggaaaga aactgagggt catcccagag
     1201 gcgcggcagt gcgcggccca gcgcttgctg gcgctgcggc aatagcgacg cggcaaagac
     1261 ggcctgcaat ttttgcagcc cggtcatggc gtcgtcgtcg cggatatggg tcagcgtggc
     1321 cgtaaactcc gcccccatgc ggtcgcccac cttggcggca atttcctctt tggatttaaa
     1381 gtggtggtac acggctccct tggaaagtcc tgtttcgtca atgacatcct gcaaggtggt
     1441 gtgggcatag cccttttggg caaacaggca tgcggcagcg tcaaggattt tttctacggt
     1501 ttgctccgga tatttgttgc gcgacatagg atacactcct ttttacagac taatggtctg
     1561 taaattgaat atacgcgctt tcacaccaaa agtcaataca tttgccctgg attgtccggt
     1621 gatttgcggc agcgggggtg gtatactttt gagagtagaa agaggggtta ttgtatggaa
     1681 aaaatcggag tcatcgatgt aggtggcggt ctgcgcggca tctatgcggc gggcgtgttt
     1741 gactggtgca tggacaacgg cgtacaattt gattacggca tcggcatttc ggcaggaagc
     1801 gctaaccttg cctcctattt aagcagccag cgcgggcgga actatctgtt ctatgaggaa
     1861 tactccatgc gcaaagaata tatgggcgca ggaaatatgc tgcacggcgg gtcgtttttg
     1921 aacctgcaat atatctacgg cacattgagt aaccgcgaat gtgaaaaccc gctggatttt
     1981 gaaaggatga cggctaatcc gatggagtgg tacatgatcg ccacagacgc cacaactggc
     2041 aagccgaagt actttacacg cggcgatatg cgccaggacg actaccgcgt gctgatggca
     2101 tcctgctgca tcccggtggc gtgcaagcct attgagattg gcggtgtgcc ttattatgac
     2161 ggtgcgctgg gtgatacgat ccctttgcaa aaggcttttg cggacggctg cgacaaggtt
     2221 gtgctgattt tgacaaagcc ggtgggcacc atccgcgcgg atggcacgga ccgcaagctg
     2281 gcgcgggtca tccaccgaaa atacccccac gccgccgaac agctggccct gcgggcgcag
     2341 cattataacg aaggtattgc gttggcccaa aagctggccg ccgagggcaa ggtgcttatt
     2401 attgcgccag atgatacctg cggcgtcaag acgctgaccc gcgaccagaa ggcactgaaa
     2461 aaactgtaca gcaaaggcat gaaagacgca gagcgcatcc gggactttct gtaattacgc
     2521 cagattcagc cgcttggtca tgaaccactg ggtcagtgcc cacataaggg catcgacgag
     2581 ggcgatgagc gcaaagacgg ccagcatgga aagcagcagg aaaggcagac tgttgtcata
     2641 gctaaaaatg gacgatgcgg tgctgaaatt gatgttgctg aaatattggt atccatagag
     2701 ggcaacgaaa aacgcagtta ccagtgccgc catctgcccg gcaaaaccga tgacgaagta
     2761 agcggcgatg ctgataagca tgcgctgctg ggtgagctgc ccgatgcaaa cggcgtgggt
     2821 gaagtgggca atcccaagt
//
LOCUS       DBMDSQ010000253         2834 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_7899_length_2834_cov_1.733260, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000253 DBMDSQ010000000
VERSION     DBMDSQ010000253.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 2834)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2834)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2834
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7899_length_2834_cov_1.733260"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..166)
                     /locus_tag="ACZPAX_08520"
     CDS             complement(<1..166)
                     /locus_tag="ACZPAX_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015518459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HIR1113980.1"
                     /translation="MLTLDKIYHAKFILKQVARKTDLIAAPRLCPGTDLYLKTENLQV
                     TGSFKVRGAYY"
     gene            450..1295
                     /gene="kduI"
                     /locus_tag="ACZPAX_08525"
     CDS             450..1295
                     /gene="kduI"
                     /locus_tag="ACZPAX_08525"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005931438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HIR1113981.1"
                     /translation="MDIRYSCNQRDFKRYTTEETRNEMLITGLYKADEVVAVYSHVDR
                     MVTLGCMPVHEEVPIDKGIDVWANFGTHYFLERREIGIFNIGDGAGTITADGQEYHLN
                     YKDCLYITKGNEKVTFKSDDPEHPAKFYMVSAPAHCSYENKLITIEQAAKRPLGSVET
                     CNKRTINQFIHPSVLKTCQLSMGMTALEPGSNWNTMPSHTHERRMEIYTYFEVPEGQV
                     VFHMCGEPTETRHIVMHNYDAVISPSWSIHSGVGTSNYTFIWAMGGENMEFDDMDNIA
                     TTDLR"
     gene            1445..2254
                     /locus_tag="ACZPAX_08530"
     CDS             1445..2254
                     /locus_tag="ACZPAX_08530"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015564554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HIR1113982.1"
                     /translation="MSVCTPKSFDLTGKVALITGASYGIGFAIATAMANCGAKIVFND
                     IKQELVDKGLAAYKEAGIEAHGYVCDVTNEDAVNAMVKQITEEVGHINVLVNNAGIIK
                     RIPMIEMTAAQFRQVIDVDLNAPFIVAKAVIPDMIKQGGGKIINICSMMSELGRETVS
                     AYAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLREIQPDGSRHPFDQF
                     IVAKTPAARWGEAEDLGGPAVFLASEASDFVNGLILYVDGGILAYIGKQPG"
     gene            complement(2535..>2834)
                     /locus_tag="ACZPAX_08535"
     CDS             complement(2535..>2834)
                     /locus_tag="ACZPAX_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005920783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase"
                     /protein_id="HIR1113983.1"
                     /translation="PAAPETITLTRKEGSADSIRLLTGVKSPMQLTDAGNGHKVTVAF
                     DESVFSNGVLWQQDAESFVCMEPWNGWANSVNEEGRHIELAPGATKEFKWSVTIE"
BASE COUNT          637 a          836 c          753 g          608 t
ORIGIN      
        1 tgtagtacgc gccgcgcacc ttgaagctgc cggtgacttg caggttctca gttttcaggt
       61 agaggtcagt gcccgggcag aggcgcgggg cggcaattaa atccgttttg cgggcgacct
      121 gctttaaaat gaacttggcg tggtagattt tatccagtgt aagcatggcg tgctccttca
      181 tttttgacaa gaattcagcc cgacccggga cttgccggat cgggccgggc cgcacccttg
      241 cggccgggac actgacatga gtatagttga aatttgctaa tttgtaaagt gaaatatttt
      301 catgattaaa ctgcacaatt aggctgctta attattgtgc aattctacca ctatgttcta
      361 acccgaggat gttgtataat aggactaacg atggcaaaat atgtttgctt tttaacaatc
      421 atctgttacg tcaaggaggc agtttccgta tggatatccg ttattcctgc aaccagcgcg
      481 atttcaagcg ctacaccacc gaggaaaccc gaaacgaaat gctcatcacc ggcctgtaca
      541 aggcggacga ggtcgtggcc gtgtacagcc acgtggaccg catggtgacg ctgggctgca
      601 tgcctgtaca tgaggaagtc cccatcgaca agggcatcga cgtctgggcc aacttcggca
      661 cccattactt cctggagcgc cgcgagatcg gcattttcaa tatcggcgac ggggccggca
      721 ccatcacggc ggacggccag gagtaccacc tgaactacaa ggactgcctg tacatcacga
      781 agggcaacga aaaagttact tttaagagcg acgatcccga gcacccggcc aaattctaca
      841 tggtttctgc cccggcccat tgcagctacg agaacaagct catcaccatt gaacaggccg
      901 ccaagcgccc cctgggcagc gttgagacct gcaacaagcg taccattaac cagtttatcc
      961 accccagcgt cctcaagacc tgccagctgt ccatgggtat gaccgccctg gagccgggta
     1021 gcaactggaa caccatgccc agccacaccc acgagcgccg catggagatc tacacctact
     1081 ttgaagttcc cgagggccag gtcgttttcc atatgtgcgg tgagcctacc gagacccgcc
     1141 acatcgtcat gcacaactac gatgccgtca tcagcccctc ctggagcatc cacagcggcg
     1201 taggcacgtc gaattacaca tttatctggg ccatgggcgg cgagaacatg gagtttgacg
     1261 acatggacaa tatcgccacc accgacctgc gctgaacgca aaaaacgcac atatcttgcg
     1321 ttgacttgcg caagaaatgc gcaacaaatt ttgcgcacca cgcatcccgt ttacttaaat
     1381 tcatgcgccc gagatacgga cgcttgaata tatgactgtt acaaacttta ggaggttttg
     1441 catcatgtct gtttgcaccc cgaaatcctt cgacctgacc ggcaaggtcg cgctcatcac
     1501 cggcgcttcc tacggcatcg gctttgccat tgctaccgct atggcaaact gcggtgccaa
     1561 gatcgtcttt aacgacatca agcaggagct ggttgacaaa ggcctggccg cctacaaaga
     1621 ggctggcatt gaggcccacg gctatgtctg cgacgtcacc aatgaggacg ccgtcaacgc
     1681 catggtcaag cagattaccg aggaagtcgg ccacatcaat gtgctggtca acaacgccgg
     1741 tatcatcaag cgcatcccga tgatcgagat gaccgctgcc cagttccgcc aggtcatcga
     1801 cgttgacctg aacgctccct tcatcgttgc caaggccgtt atccccgata tgatcaagca
     1861 gggcggcggc aaaatcatca acatctgctc catgatgagt gagctgggcc gcgagactgt
     1921 ctccgcctat gctgctgcca agggcggcct gaagatgctg accaagaaca tcgcttctga
     1981 gtacggccag tacaacatcc agtgcaacgg catcggacct ggctacatcg ccaccccgca
     2041 gactgccccc ctgcgcgaga tccagcccga tggctctcgt catcccttcg accagttcat
     2101 cgttgccaag acccccgctg cgcgctgggg tgaagccgag gatctgggcg gccccgccgt
     2161 cttcctggcc tctgaggctt ccgactttgt caacggcctg atcctctacg tcgatggcgg
     2221 catcctggcc tacatcggca agcagccggg ctgatacttt cttcggagaa gaaagtaatc
     2281 aaagaagctt tcaaaattta tcccatgaaa gggatatata ataaagcaaa aaggacatcc
     2341 gctcgtaatt ggcggatgtc cttttttgtt gcacggtagg ggccgggcat gcccggcccg
     2401 caactgtacg gcaaaagcgc ttttacggta aggctgcggg ccgcacacgt gcggccccta
     2461 cctcgcaacg cttgtagggg cggcgtcctt gatgccccgc agaagggctg aatctctccc
     2521 ctactgccgt aactttactc gatggtcacg ctccacttga actctttcgt cgcgccgggg
     2581 gccagctcga tgtggcggcc ttcctcattg acggagtttg cccagccgtt ccacggctcc
     2641 atgcagacga agctctcggc gtcctgctgc cagagtacgc cgttggagaa aacgctctcg
     2701 tcaaaggcaa ccgtgacctt gtggccgttg ccggcatcgg tcagctgcat cgggcttttt
     2761 acgccggtca gcagacggat ggagtcggcg ctgccttcct tgcgggtcag cgtgattgtc
     2821 tcgggcgcgg cggg
//
LOCUS       DBMDSQ010000254         2829 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_7917_length_2829_cov_1.428886, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000254 DBMDSQ010000000
VERSION     DBMDSQ010000254.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 2829)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2829)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2829
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7917_length_2829_cov_1.428886"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..378
                     /locus_tag="ACZPAX_08540"
     CDS             <1..378
                     /locus_tag="ACZPAX_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR1113984.1"
                     /translation="RAYYITGDEAHGSSIFAKITQAEQDGKKTFPFTSGKNLYDFIDV
                     DELANMIVAASVQDEINGIINVCTGKPMSLAERVEQFLKDKNYKIRLNYGAFPDRPYD
                     SPGTWGDPAKINKILSMDTYKKA"
     gene            402..2345
                     /locus_tag="ACZPAX_08545"
     CDS             402..2345
                     /locus_tag="ACZPAX_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnan synthesis F family protein"
                     /protein_id="HIR1113985.1"
                     /translation="MKRLGIFFFYEKNGDVDDFITYYLADLNKNLTELVVVCNGKLSA
                     QGRAAFEQFTDNIIVRENKGLDVWAYKTALDSYGWAKLTEFDEIVMTNSTLMGPVRPL
                     KEMFDAMWENQDLDFWGLSIHHGAKSNPFKGKHLYNYLPVHIQSHFIVYRQRFVKAPE
                     LQNYWDNMPMIESYADSVQRYEAVFTKQFEDKGFKWDVYVKTDDLKEFTDYPLLVCPT
                     RLLREKKCPLFKRRSFMHELEAYLNYTAGEPVQELYAYLRDETDYPMALIWKNMIRTM
                     HPHDFTRSLALTRIIEPTVLDAVTAESICKNRRIALAMHLYFMDMLDQSKAFAAKFPP
                     ETDVFISTSSAEKKPQIEAAFADLNLHSVTVTVVENQGRDVAAFLCDLAPQLKDYDYA
                     CFMHDKKAIQTKPGSVGASFGYVCNENVCKNAAHVLNVLCEFEKDPYLGILCPPYPTH
                     GLYFMNMCSGGWGPNFENTKKLMKDLGIDIPISGEKSPIAPYGSVFWFRPKALEPLFA
                     HGWQHSDFPPEPLPQDGTISHAIERIYPFVAQSAGYYPAVVMSKNYAVTHNDTMQAYA
                     SGMIRPLARVFDCTTFYGAENSATGFAYKKHHLFSHYGPYSDSKRRHARNWLRDNLPA
                     GSYKVIINTKRAIFGPHHGPYED"
     gene            2351..>2829
                     /locus_tag="ACZPAX_08550"
     CDS             2351..>2829
                     /locus_tag="ACZPAX_08550"
                     /inference="COORDINATES: protein motif:HMM:NF016903.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnan synthesis F family protein"
                     /protein_id="HIR1113986.1"
                     /translation="MKRLIVYFHYDPLGQIDTACRVAMEAMAHYGEVFFISNGTLRPA
                     DRAWAASVTLTCRERENKGLDVGAYKEALAVIGRGRLARYDELVLMNFTLAGPVCSLA
                     SMFAAMEARPELAFWGLTRHYAMKSRRFGGRSGEVPEHLQSHFLAVRAPLLHSEDFW"
BASE COUNT          653 a          851 c          768 g          557 t
ORIGIN      
        1 cgggcctact acatcaccgg cgacgaagcc cacggcagct ccatttttgc aaaaatcacc
       61 caggccgagc aggacggcaa aaagaccttt ccgttcacca gcggcaagaa cctctatgat
      121 ttcatcgatg tggacgagtt ggcaaacatg attgtggcgg ccagcgtgca ggatgagatc
      181 aatggcatta tcaacgtctg caccggcaag ccgatgagcc tggccgagcg ggtcgagcaa
      241 ttcctcaaag acaagaacta taaaatcagg ctcaactacg gcgcattccc ggatcgcccc
      301 tacgacagcc cgggcacttg gggtgacccg gccaagatca ataagattct gtccatggat
      361 acctacaaaa aagcataaaa gagcataaaa ggaaaaacgc aatgaaacga ttgggcatct
      421 ttttctttta cgaaaaaaac ggcgatgtgg atgatttcat cacctactac ctcgctgacc
      481 tgaataagaa cctgaccgag ctggtcgtcg tctgcaacgg caagctcagc gcacagggcc
      541 gcgcggcctt tgaacagttt accgacaaca tcatcgtgcg cgagaacaag ggcctcgacg
      601 tctgggccta caaaacggcg ctggattctt acggctgggc gaagctcact gaatttgacg
      661 agatcgtcat gacgaactcg accttgatgg gtccggtgcg cccgctcaag gaaatgtttg
      721 acgccatgtg ggaaaatcag gatctcgatt tctggggtct gtctatccac cacggcgcaa
      781 agtcgaaccc cttcaagggc aagcaccttt ataactatct gccggtgcac atccagtccc
      841 atttcatcgt ctaccgccag cgctttgtca aggcccctga actgcaaaat tattgggaca
      901 atatgccgat gatcgaaagc tacgccgact cggtgcagcg gtacgaggcc gtgttcacca
      961 aacagtttga ggataagggc ttcaagtggg acgtctatgt caagaccgac gatttgaagg
     1021 agttcaccga ctatccgctg ctcgtctgcc cgacgcgcct tctgcgggag aaaaagtgtc
     1081 cgctgttcaa gcgccgcagc tttatgcacg agctggaagc ctacctcaac tatacagcgg
     1141 gggaaccagt gcaggaactg tacgcttacc tacgggatga gacagactat ccgatggccc
     1201 tcatctggaa gaacatgatc cgcacgatgc acccccacga cttcacccgc agcctggccc
     1261 tgacccgcat cattgagccg actgtgctgg atgccgttac ggcggagagt atctgcaaaa
     1321 accgccgcat tgcgctggcc atgcacctct attttatgga tatgctcgac cagagcaagg
     1381 cgtttgcggc caagttcccg ccggagacgg acgtttttat ctcgacgagc agcgccgaaa
     1441 aaaaaccgca gatcgaagcg gcctttgctg atttgaacct gcacagcgtc accgtgaccg
     1501 tggtggaaaa ccagggccgc gatgtggccg cgtttctgtg cgacctggcc ccgcagctca
     1561 aagactacga ctatgcctgc tttatgcacg acaaaaaggc catccagacc aagccgggca
     1621 gtgtgggggc cagcttcggc tatgtctgca acgagaacgt ttgcaaaaat gcagcccatg
     1681 tgctgaacgt gctgtgtgaa tttgaaaagg atccctacct cggcattctc tgcccgccgt
     1741 acccgacgca cggcttgtac tttatgaata tgtgctccgg cggctggggc ccgaactttg
     1801 agaacacgaa aaagctgatg aaagatctgg gcatcgatat acccatttcc ggcgaaaaat
     1861 ctcccattgc gccctacggc agtgtgttct ggttccgccc caaggcgctg gaaccgctgt
     1921 ttgcccacgg ctggcagcat tcggacttcc cgcccgaacc cttaccgcag gacggcacca
     1981 tcagccacgc catcgagcgc atttacccct ttgtggccca gagcgcgggc tactacccgg
     2041 cggtggtcat gagcaaaaac tatgccgtga cccacaacga cacgatgcag gcctacgcca
     2101 gcggcatgat ccgcccgctg gcccgggtgt tcgactgcac gaccttctac ggtgcggaaa
     2161 actcggccac aggctttgcc tacaaaaagc accacctgtt cagccactat gggccgtact
     2221 ccgacagcaa gcgccgccac gcgcggaact ggctgcggga caacctgccc gcaggttcct
     2281 acaaggtcat catcaacacc aagcgtgcca tcttcggccc ccaccacggc ccgtatgagg
     2341 attaagcccc atgaaacggc tgatcgttta tttccattat gacccgctgg gccagatcga
     2401 caccgcctgc cgtgtggcca tggaagccat ggcccattat ggcgaggtgt tctttatctc
     2461 caacggcacg ctgcgcccgg cggaccgtgc ctgggcggcg tcggtgacat tgacctgccg
     2521 cgaacgggaa aataagggcc ttgacgtggg tgcctataaa gaagcgctgg ctgtcatcgg
     2581 ccggggccgc ctggcccgct acgacgaact ggtgctgatg aacttcacgc tggcaggccc
     2641 cgtctgctcg ctggcctcga tgttcgctgc catggaggcc cgcccggagc tggccttttg
     2701 gggcttgacc cgccactacg ccatgaagag ccgacgcttt ggcggccgca gcggggaagt
     2761 gccggaacat ctgcaatccc actttttggc cgtccgcgca ccgcttttgc acagcgagga
     2821 tttctggca
//
LOCUS       DBMDSQ010000255         2820 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_7947_length_2820_cov_1.823832, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000255 DBMDSQ010000000
VERSION     DBMDSQ010000255.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 2820)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2820)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2820
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7947_length_2820_cov_1.823832"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            185..655
                     /locus_tag="ACZPAX_08555"
     CDS             185..655
                     /locus_tag="ACZPAX_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005939792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIR1113987.1"
                     /translation="MDITERKITKIAREAEKLVLCTLREQGVGTAEIDLIHALRHHPG
                     CTQAALAELLHADKAAIARRTKNLEAKGYLVREDAPNDRRSQLLYPTGKAENLKSSKA
                     EIEAAFYGYLTAALPADEAAAFAATLDKLYHRSKTESRAGFPHFQRECKTDDEA"
     gene            645..2387
                     /locus_tag="ACZPAX_08560"
     CDS             645..2387
                     /locus_tag="ACZPAX_08560"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005939795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1113988.1"
                     /translation="MKREKQFRPDKIGDYFRIEWLPLVLVTVSGLIYNIGLLAAPWFE
                     GRLAQCLADILGGNAAASAMAMLVLAYILVTLLVQAARFIKRFYVRRFANNINRRMKG
                     VLYANLVRQSRAALEKEGAGELMTKAISDVDDCVEGMRKFTTEVFDTGVALVGYIVML
                     LVYDWRLALLSLLFTPVSYVCAAGMKKPVQREGAAYKKATGALRDATLDRARNAATYR
                     NHGCETAREERYEDVLKNYERTAVRSNVWQSALPPLYLAVSEAGVLFILWFGAKNVLG
                     AGWCAWNIAAFTTFLSCFTKLVVKSSKVAKLFNSVQKAEVSWRRIKPLMKLPEQLAPL
                     EVPAPAAVTLENLSFAYADEPIFAGLTLTAHPGDIIGITGPVACGKSTLGRVFLCEAP
                     YGGSAKFGDKEFSNLTPRQISATVGYLGHDPELSADTVKNNLLCGGAQDIAPYLAAAA
                     LDGEVRAMEQGRDTVIGPSGTRLSGGQAQRLALARTLAHPRPVLVLDDPFSALDRHTE
                     DTVFANLQIYAKDKVVFLISHRLYHFPQMRQVIFMEDGKTTVGTHDKLMAAVPAYRQL
                     YESQTGGQQNENEQ"
     gene            2371..>2820
                     /locus_tag="ACZPAX_08565"
     CDS             2371..>2820
                     /locus_tag="ACZPAX_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005939798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HIR1113989.1"
                     /translation="MKTSNKNGVAAALRTAAMAHCFLTVGVILCVAASVAASLLPPLL
                     LARCIDSLTAGLPLTIWAVLAYFASLALEGVLSSAQESLLVVFGQKMTHALRLEMSHK
                     LTRLPAATLVDQNPGEVAARFSGDVDTVEALFTSGIISMVADACRIIS"
BASE COUNT          555 a          848 c          872 g          545 t
ORIGIN      
        1 gaaaagtgcg aacataaaaa cagtttcatc aatattctcc tttatatata aattccgatt
       61 tatagttcta agtataccat atccagacct tgtgcgccac tctattgaca tggcgcaaaa
      121 ccgggctata atggttgtag gtacaacgat gtagatacat ctttctgaag cccgggaggc
      181 gtctatggac attaccgaac gaaaaatcac aaaaattgcg cgtgaggccg aaaaactcgt
      241 cctctgcacc ctgcgggaac agggcgtggg tacggcggaa atcgacctga tccacgccct
      301 gcgccaccac cccggctgca cccaggcggc cttggccgag ctgctgcacg ccgacaaggc
      361 ggccatcgca aggcgcacga aaaaccttga ggccaagggc tatcttgtcc gcgaagatgc
      421 ccccaacgac cggcgcagcc agcttttgta cccgaccggg aaggccgaga acctgaaatc
      481 ctcgaaggca gagatcgagg cggccttcta tgggtatctg accgccgcgc tgcccgccga
      541 cgaggccgct gcctttgcgg caacgctgga taagctgtac catcggtcca agaccgagag
      601 ccgcgcgggg tttccgcatt tccaaaggga gtgcaagaca gacgatgaag cgtgaaaaac
      661 aattccgccc ggacaaaatc ggcgactatt tccgcatcga gtggctgccg ctggtgctgg
      721 tcacggtgtc gggccttatt tataacatcg gcctgctggc cgcgccctgg tttgaggggc
      781 ggctggccca atgcctggcc gacattctgg gcggcaacgc ggcagcgtcc gccatggcga
      841 tgctcgtgct ggcttacatc ctcgtcacgc tgctggtgca ggcggcgagg ttcattaagc
      901 ggttttacgt gcgccgcttt gccaacaaca tcaaccgccg gatgaagggc gtgctctacg
      961 ccaaccttgt gcgccagagc cgcgccgcgc tggaaaaaga gggcgcgggc gagctgatga
     1021 ccaaggccat ctccgatgtg gacgactgcg tcgaagggat gcgcaaattc acgaccgagg
     1081 tgttcgacac cggcgtggcg ctggtgggct acatcgtcat gctgctggtg tatgactggc
     1141 ggctggcact gctcagcctg ttgtttacgc cggtctccta cgtctgcgcc gctgggatga
     1201 aaaagccggt gcagcgcgaa ggggcggcct acaaaaaggc gacgggcgct ttgcgcgacg
     1261 ccacgctgga tcgtgcccgg aacgccgcga cctaccgcaa ccacggctgt gagactgccc
     1321 gcgaggaacg gtacgaggat gtgctgaaaa actacgagcg gaccgccgtg cgcagcaatg
     1381 tctggcagtc ggcgctgccg ccgctctacc tggctgtgtc cgaggcgggc gtgctgttta
     1441 tcttgtggtt cggcgcaaaa aatgtcttgg gcgccggttg gtgcgcctgg aatattgccg
     1501 cgttcacgac gtttttgtct tgcttcacga aactggtcgt caagtcatcg aaagttgcaa
     1561 agctcttcaa ctccgtccaa aaagccgagg tttcctggcg gcgcatcaag ccgctgatga
     1621 agctgccgga gcagctggct ccgctggagg tcccagcccc cgccgccgtg acgctggaaa
     1681 acctctcctt cgcctacgcc gacgaaccga tttttgcagg gcttactctg acggcgcacc
     1741 ccggcgatat catcggcatc accggtccgg tcgcctgcgg taaatccacg ctggggcgtg
     1801 tgttcctgtg tgaagcgccc tacggcggct cggcaaagtt cggggacaaa gagttctcga
     1861 acctgacacc gcggcaaatc tccgccacgg tcggctacct gggtcatgac ccagagctaa
     1921 gcgccgacac cgtgaaaaat aacctcctct gcggcggtgc gcaggacatc gcgccctatc
     1981 tggccgccgc cgcgcttgac ggcgaggtgc gggccatgga gcagggccga gataccgtca
     2041 tcggccccag cggtacgcgg ctttccggcg ggcaggccca gcgtttggcg ctggcccgca
     2101 cgctggccca cccccgcccg gtgttggtgc tggacgaccc gttctcggcg ctggaccgcc
     2161 ataccgagga taccgtgttt gcaaatttgc agatctacgc caaagataaa gtcgtgttcc
     2221 tgatttccca ccggctgtac cacttcccgc agatgagaca ggtaattttt atggaggatg
     2281 gaaagaccac tgtgggcacc cacgacaagc tgatggcggc ggtccccgcg taccgccagc
     2341 tgtacgaaag ccagacagga gggcagcaaa atgaaaacga gcaataaaaa cggtgtggct
     2401 gccgccctgc gcacggcggc catggcacac tgttttttga cagttggcgt gattttgtgc
     2461 gtggctgcgt cggttgcggc gtcgctgctg ccgccgctgc tgctggcacg ctgcatcgac
     2521 agtctgacgg cgggactgcc gctgacgatc tgggccgtgc tggcctactt tgccagcctg
     2581 gcgctggagg gcgttttatc cagtgcacag gagagtctgc tggtcgtttt tggtcagaag
     2641 atgacccacg cgctgcgctt ggaaatgtcc cacaagctga cccggctgcc cgccgccacg
     2701 ctggtggatc agaaccccgg cgaggtggct gcgcggttct ccggcgatgt ggacacggtg
     2761 gaggcactgt tcacgtcggg catcatcagc atggtggcgg acgcctgccg catcatttct
//
LOCUS       DBMDSQ010000256         2800 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_8014_length_2800_cov_1.823638, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000256 DBMDSQ010000000
VERSION     DBMDSQ010000256.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 2800)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2800)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2800
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8014_length_2800_cov_1.823638"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..463
                     /locus_tag="ACZPAX_08570"
     CDS             <1..463
                     /locus_tag="ACZPAX_08570"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR1113990.1"
                     /translation="VILSSHILSEVQSVCDRVLIIAHGKLVAQGTPEELAAQLTAKGT
                     ITATAQGSRDAVVAAASAVPGLTDVQVTDEKGGEVSFTAVSTAGTDLRGALSLALAQA
                     GCPVLSLSAETMSLEDVFLQLTEAPDAAGETTPAPETQTEEKEANGDDSNL"
     gene            447..1310
                     /locus_tag="ACZPAX_08575"
     CDS             447..1310
                     /locus_tag="ACZPAX_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005932810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1113991.1"
                     /translation="MTAIFKREVKSYFTGMIGWVIAAVSLFFLGLYYTNRNLLYASSD
                     FASVLYTMTMILLFLLPAISMRSFAEERKNKTDQLLLTSPVSIPAIVAGKFLAEVAVF
                     AIPLAAAVLMPLILKAFGTVSLVAAYSALLGYVLLGAACLAVGTWISALTENQILAYL
                     ATFGALLVAYLMNGIKTMFTTGNLLAFIVFTVVLLIAAILVGVICKRLTAGASVFCVG
                     AVVLFILFQLRPAWLLAAFNAVLSALALFEPFADIVGGMFSIPAIAYYLSVIGLFLFL
                     TGQALERRRWN"
     assembly_gap    1428..1437
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1438..2750
                     /locus_tag="ACZPAX_08580"
     CDS             <1438..2750
                     /locus_tag="ACZPAX_08580"
                     /inference="COORDINATES: protein motif:HMM:NF021351.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HIR1113992.1"
                     /translation="LVIRALPTKYTQYDISTTSLFTLSETTENLLHELNADVTAYYLA
                     ESGQEDDNITRLLDRYAGESSHFSWQQRDPVLYPTFAQQYDGASTGSVVLVSGDNSDV
                     LSYNDMYELDLESYYSTGSANYSFQAENAITSGIAKVTRETSYQLYELTGHGEAALSG
                     DFTDTLNNAGVTTAELNLTTAGSIPADAAAVLINAPGADLTDAETTILTDYVANGGKL
                     FVATDFTTDTPNLDTLLAACGMARQPGLLIETDTDHYPYGYPQTYLLPTLASNEITAG
                     VSQNMMVYTPIAQGITTNEDSEFSFTDLLTTGDAAYSMENYATAETAQKADTDPEGTF
                     AVAVAAENVTTGARVVWVNCPNLLLSNINQAVSGGNAQFLGSVVNWMNGEQTTAVISA
                     KSMSAASLSVPTSAALPLGVLFTLVLPIICLIAGAVVCVVRRRR"
BASE COUNT          528 a          895 c          836 g          531 t
ORIGIN      
        1 ggtcattttg tccagccata ttttgagcga ggtccagtcc gtctgtgacc gggttctcat
       61 cattgcccac ggcaagctgg tggcccaggg gacgccggag gaactggccg cccagctgac
      121 cgcgaaggga accatcaccg ccacggctca gggcagccgc gatgccgttg tggccgctgc
      181 atccgctgtg cccggcctga ccgatgtaca agtcaccgac gaaaagggcg gcgaggtcag
      241 ctttaccgcc gtcagcactg cgggaacaga cctgcgcggt gcactcagcc tggcgctggc
      301 ccaggcgggt tgcccggtgc tgagcctgag cgccgaaacc atgagcctgg aggatgtgtt
      361 cctgcagctg accgaagccc cggacgcggc aggggagacg acccccgcac ccgagacgca
      421 aaccgaggaa aaggaggcga acggcgatga cagcaatctt taagcgtgag gtcaaaagtt
      481 actttaccgg tatgatcggc tgggtcatag cggcggtcag tctgttcttt ttgggactgt
      541 actacacgaa ccgcaacctg ctctacgcct cgtcggattt cgcgtcggtg ctctacacca
      601 tgacgatgat cttgctcttc ctgctgccgg ccatcagcat gcgcagcttt gccgaggaac
      661 gcaagaataa gaccgaccag ctgcttctga ccagcccggt cagtatcccg gccatcgtgg
      721 cgggcaaatt tctggccgag gtcgccgtgt tcgccatccc gctggcggcg gcggtgctca
      781 tgccgctgat tttaaaagcc ttcggtacgg tatcactggt ggcagcgtac agcgccctgc
      841 tgggctatgt cctgctgggc gcagcctgcc tggccgtggg tacttggatc tcggctttga
      901 ccgagaacca gattctcgcc taccttgcca ccttcggtgc gctgctcgtc gcttatctga
      961 tgaacggtat caaaacgatg ttcaccaccg gcaacctgct ggcgttcatc gtgttcaccg
     1021 tggtgctgct gattgcggcc atcctcgtgg gtgtcatctg caagcggctg acggcaggcg
     1081 cttcggtgtt ctgcgtcggc gcggtcgtgc tgttcatcct gttccagctg cgcccagcgt
     1141 ggctgctggc ggccttcaac gccgtactgt cggcgctggc cctgtttgaa ccctttgcgg
     1201 acatcgtggg cggtatgttc agcatcccgg ccattgccta ctatctgtcc gtgattgggc
     1261 tgttcctctt tttgaccggg caggcgctgg aacgccgccg ctggaactaa gggaggtgcc
     1321 gcacaatgaa aaaaccaaat ctgaaattcc cgcagggcgg cagcaaacgc gccctgcaca
     1381 gcggcctgta cgcaagcgtg ctggccgccg tggcgctggt cgtggtcnnn nnnnnnnacc
     1441 tggttatccg cgccctgccc accaaataca cccagtacga tatttctacg acctcgctgt
     1501 ttaccttgag tgagacgacc gagaacctgc tgcatgagct gaacgccgac gtgacggcct
     1561 actacctggc cgagagcggg caggaggacg acaacatcac ccgcctgctg gaccgctatg
     1621 cgggcgagag cagccatttc agctggcagc agcgcgaccc ggtgctttac ccgaccttcg
     1681 cccagcagta cgacggggcc tccaccggca gtgtcgtcct cgtcagcggc gacaacagcg
     1741 atgtgctgtc ctataacgat atgtacgagt tggatctgga gagctactac tccaccggca
     1801 gcgccaatta cagcttccag gcggaaaacg ccattacctc cggcattgcc aaggtcacgc
     1861 gggagacgtc ctaccagctg tacgagctga ccggccacgg cgaggcggcc ctgtccggcg
     1921 actttaccga cacgctgaac aacgcgggcg tcaccactgc cgagctgaac ctgaccacgg
     1981 cgggcagtat cccggcggat gccgctgcgg tgctcatcaa tgcacccggg gccgacctga
     2041 ccgacgcaga aacaacgatt ttgactgact acgttgcaaa cggcggcaag ctgtttgtgg
     2101 ccaccgactt taccaccgac acgcccaacc tggacacgtt gctggccgcc tgcggcatgg
     2161 cccgtcagcc gggtcttttg atcgagacgg acaccgacca ctacccctat ggctaccctc
     2221 agacctacct gctgcccacg ctggccagca acgaaattac ggcgggtgtg tcccagaata
     2281 tgatggtcta cacacccatc gcccagggca tcaccaccaa tgaggacagc gagttcagct
     2341 ttaccgatct gctgaccacc ggcgacgcag cctactcgat ggagaactac gccaccgccg
     2401 agaccgccca gaaagccgac accgaccctg agggcacctt tgctgtggcc gtggcggcgg
     2461 agaacgtcac cactggcgca cgggtcgtct gggtcaactg cccgaacctg ttgctgtcca
     2521 acatcaacca ggccgtctcc ggcggcaacg cgcagtttct gggcagcgtc gtcaactgga
     2581 tgaatggcga acagacgacc gccgtcatca gcgctaagag catgagcgcg gcctcgctga
     2641 gcgtgccgac ctcggccgca ctgccgctgg gcgtgctgtt cacgctggtg ctgcccatca
     2701 tctgcctgat tgcgggtgcg gtggtttgcg tcgtgcgccg ccgcagataa ggagccgcca
     2761 tgaaaaacac aaaaaagatg ctgccgctgg tggtgctgat
//
LOCUS       DBMDSQ010000257         2799 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_8019_length_2799_cov_1.543365, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000257 DBMDSQ010000000
VERSION     DBMDSQ010000257.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 2799)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2799)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2799
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8019_length_2799_cov_1.543365"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..380
                     /locus_tag="ACZPAX_08585"
     CDS             <1..380
                     /locus_tag="ACZPAX_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HIR1113993.1"
                     /translation="IISEILEPNNIPYVETRSMEEALPDLDVLYMTRVQRERFFNEED
                     YIRLKNSYVLTKEKLALAPADMPVLHPLPRVNEITLDVDDDPRAAYFDQVQNGVYMRM
                     ALIMTLLGLKDPKTGEVAFNVEG"
     gene            367..795
                     /locus_tag="ACZPAX_08590"
     CDS             367..795
                     /locus_tag="ACZPAX_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HIR1113994.1"
                     /translation="MLKVDEIQNGVVIDHITAGTGLALYHMMELEKLTNASVALLQNV
                     RSQKDGKKDIIKIEGDVSGLNFDILAYVDPKISITFIKNGHVTEKKHPEQPKRLVNVI
                     KCTNPRCITSIEEGCDHIFALTPSGRYRCIYCEQEFRVKP"
     gene            809..1012
                     /locus_tag="ACZPAX_08595"
     CDS             809..1012
                     /locus_tag="ACZPAX_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016730 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-thioredoxin reductase catalytic
                     domain-containing protein"
                     /protein_id="HIR1113995.1"
                     /translation="MDKSRFHLNPDAEIVKNIREGLKRTGGYCPCRLQHIPENICICK
                     EFKEQLADPNYHGPCHCGLYVKD"
     gene            complement(1057..>2799)
                     /locus_tag="ACZPAX_08600"
     CDS             complement(1057..>2799)
                     /locus_tag="ACZPAX_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR1113996.1"
                     /translation="QEGRLDPVLCREAELDRMIEILCRRQKNNPCLLGEPGVGKSALA
                     EALAQRIASGQITPALRGKRLLALDMASMVAGTKYRGDFEERFKNLLEELYRDRSTVL
                     FIDEIHIIAGAGAAEGAIDAASILKPMLARGEIQLIGATTPEEYRKTIQKDSALERRF
                     GKVMVEEPAPTDAEKILNGLMPRYERYHGIAIPPQAIHAAVELSVRYLPGRFLPDKAI
                     DLLDEAAAARRIADTGGEKKALSPADIAHVVSKASGVPAERVGEAERERLAHLEERLS
                     AEVIGQSRAVAAVASAIRRSRTGLRESGRPIGAMLFLGPTGVGKTQLARTLAKCWFGS
                     EKALLRFDMSEYMERHTVARLIGAPPGYLGHDEGGQLTEAVRRRPYSVVLFDEIEKAH
                     SDIQNLLLQILEDGSLTDAQGRKSDFSNTIILLTSNLGARCLSGQTAPLGFGAATSET
                     SRRSQQAVQEAKEFFRPELMGRLDETVLFDPLGPAQLEGIAGRLLCELEQRAAAQGYT
                     LRHTAAAAKTLAGDKVPPYGARELRRTVSRAVEQALADRIAAGTAHPGVVYTADADAE
                     GNIILTEGTLAACV"
BASE COUNT          569 a          849 c          811 g          570 t
ORIGIN      
        1 acatcatcag cgagatcctc gaacctaaca acatccccta tgttgagaca cgcagcatgg
       61 aggaggctct gcccgatttg gacgtcctct acatgacccg cgtccagcgt gagcggttct
      121 ttaatgaaga agattacatc cgcctgaaaa acagctatgt cctgacaaag gaaaagctgg
      181 ctctggcccc cgcggatatg ccggtgctgc atcccctgcc ccgtgtgaat gagatcacgc
      241 tggatgtaga cgacgatccg cgtgcggcct actttgacca ggtgcagaac ggcgtgtata
      301 tgcgtatggc gctgatcatg actttgctgg gtctgaaaga ccccaagacc ggggaggtag
      361 cgttcaatgt tgaaggttga tgagattcag aacggtgtgg ttattgacca catcaccgca
      421 ggcaccggcc tggccttgta ccacatgatg gaactggaaa aattaaccaa tgccagtgta
      481 gcacttttgc agaatgtccg cagccaaaag gacggcaaaa aggatataat aaagattgag
      541 ggcgacgtgt ccgggctgaa ctttgacatc ctcgcctatg tcgatccgaa aatctccatc
      601 acctttatca agaacggcca cgtgacggag aagaagcacc ccgagcagcc aaagcgcctg
      661 gtcaatgtca tcaagtgcac gaacccgcgc tgcatcacct ccattgagga aggctgtgac
      721 cacatctttg ctttgacgcc cagcggccgt taccgctgca tctactgcga acaggagttc
      781 cgcgtcaagc cgtaaacgag gtaacattat ggataagtca agatttcacc tgaatcctga
      841 tgccgagatc gtgaagaaca tccgcgaggg gctcaagcgc accggcgggt attgcccctg
      901 ccgcttgcag cacatcccgg agaatatctg catttgcaaa gagttcaaag aacagctggc
      961 cgacccaaac taccatgggc cgtgccactg tgggctgtac gtgaaagatt gacagagaaa
     1021 cgaggtatct gctgagatgg caggtacctc atttttttat acacacgccg ccaacgtacc
     1081 ctcagtcaaa ataatattcc cttccgcatc ggcatcggcg gtgtaaacga cgccggggtg
     1141 ggcggtcccg gcggcaatac ggtcagcgag ggcttgctcc actgcgcggc tgacggtgcg
     1201 gcgcagctcc cgcgcaccgt agggcggtac tttgtccccc gccagcgttt tggcagcggc
     1261 agcggtatgg cgcagcgtgt agccctgtgc ggcggcgcgc tgttccagtt cgcacagcag
     1321 ccggccggca atgccctcca gctgtgccgg gccgagcggg tcaaacaaaa ccgtctcgtc
     1381 cagacggccc atcagttcgg ggcggaaaaa ttcttttgcc tcctgcacgg cctgctggct
     1441 gcggcggctc gtctcggagg ttgccgcacc gaagcccagc ggggccgtct gtcctgaaag
     1501 acaccgcgcc cctaaattgg acgtgagcaa aataattgtg ttggaaaaat cagattttcg
     1561 cccctgcgcg tcggtcaggc tgccatcctc gaggatttgc agcaatagat tttggatatc
     1621 gctgtgtgcc ttttcgattt cgtcaaacag tacgacgctg tatggtcggc ggcgcacggc
     1681 ctcggtcagc tggccgccct cgtcatggcc caaatagccc ggcggcgcac cgatgagccg
     1741 cgccacggtg tggcgctcca tatactccga catatcaaag cgcagcagag ctttctcgct
     1801 gccgaaccag cattttgcca gcgtgcgggc cagctgcgtt ttacccacac ccgtcggccc
     1861 caaaaacagc atggcaccga tggggcgccc gctctcccgc aagccggtac ggctgcggcg
     1921 gatggctgat gccacggcgg cgacggcccg tgactggccg atgacctcgg cagaaaggcg
     1981 ctcttccaag tgtgccagcc gctcacgctc ggcctcgcct acccgttcgg cgggcacgcc
     2041 gctggccttg ctgaccacat gggcgatgtc ggcggggctt aacgcttttt tctcgccgcc
     2101 ggtgtcggca atgcggcggg cggcggctgc ttcatccagc aggtcaatgg ccttgtcggg
     2161 caggaaacgc cccggtaaat agcgcacgct caactccacc gccgcatgga tggcctgcgg
     2221 tgggatagca atgccgtggt agcgctcgta ccggggcatc aggccgttta agattttttc
     2281 ggcgtcggtg ggggcgggtt cctccaccat gacctttcca aaacggcgtt ccagcgccga
     2341 atctttttga attgttttgc ggtattcctc cggcgtcgtt gcgccgatga gttgtatctc
     2401 cccgcgggcc agcatcggtt ttaggatgct cgcggcgtcg atggctccct ccgccgcacc
     2461 ggccccggca ataatgtgta tctcgtcgat gaacagcacg gtggagcggt cgcggtacag
     2521 ttcttccaat agatttttga accgttcctc gaaatcgccg cggtactttg tacccgcaac
     2581 catggatgcc atgtccaacg ccaacaaccg cttgccccgc agcgccgggg tgatttgccc
     2641 gctggcaatg cgctgggcca gcgcctcagc cagggcactt ttgccgacgc caggctcccc
     2701 cagcaggcag ggattatttt tctggcggcg gcagagaatt tcaatcatcc ggtccagctc
     2761 ggcttcccgg cagagcaccg ggtccagccg cccctcctg
//
LOCUS       DBMDSQ010000258         2789 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_8051_length_2789_cov_1.271205, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000258 DBMDSQ010000000
VERSION     DBMDSQ010000258.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 2789)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2789)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2789
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8051_length_2789_cov_1.271205"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..616)
                     /locus_tag="ACZPAX_08605"
     CDS             complement(<1..616)
                     /locus_tag="ACZPAX_08605"
                     /inference="COORDINATES: protein motif:HMM:NF024085.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3794 domain-containing protein"
                     /protein_id="HIR1113997.1"
                     /translation="MELKVFKDTIASYGGRWETRLELPVETEILIPDYLPAVFKIVKC
                     LIAPVVLQNRVAGGRWQVEGYLRCTVFYQSEEPGTRLLRTEQKFAFEKSVELPAGQYA
                     EGPAQVWGEPEYCNCRAVSEHRIDLRGAYILCAAVAVRKELELLTSLADCGIEQYTRT
                     MQGLQCAVTEEKTLTAETVAALPGAGESVLDITGSFAPLGTIWVC"
     gene            complement(843..1475)
                     /locus_tag="ACZPAX_08610"
     CDS             complement(843..1475)
                     /locus_tag="ACZPAX_08610"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015538483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HIR1113998.1"
                     /translation="MSKRAIILSAVPVSTAVGRYVRPGDFIVACDAGYRNAERLDLRP
                     DLIVGDFDSAPRPRAEHETIVLPHVKDDTDTQYAARWLLEHGYDEITLLGALGGARLE
                     HTLANLATGLYLAKNGVKVLLANERSELHFLVPNTPMELHRSDWKYVSVFPLEGQLTG
                     LAIRGAFYPLENAKLTADYPLGVSNEFVAETAHLQCSGGTGVVVLTRDDA"
     gene            complement(1472..2332)
                     /gene="rsgA"
                     /locus_tag="ACZPAX_08615"
     CDS             complement(1472..2332)
                     /gene="rsgA"
                     /locus_tag="ACZPAX_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HIR1113999.1"
                     /translation="MAYITKGIGGFYYVKTPDGIVECKARGIFRKRGITPVAGDNVAL
                     SADGTMIDEILPRKNVFIRPPIANLDILFIVTSTTQPVPSTLVLDELAAAAIYKDVQP
                     VLVVTKADLAAADMLRTAYTGSGIPLIQLNYETGEGLDEIRGYIQGHLCAFCGNSGVG
                     KSTLLNTLAPELKRETGSISQKLGRGRHTTREVEIFEICGGRLADTPGFASLEAQKLC
                     RIPKDDIQHTFPEFAPYFGQCRFTGCSHRSETGCAVREAVDSGTISKTRYASYLAMYE
                     EASARKEWEK"
     gene            complement(2347..>2789)
                     /locus_tag="ACZPAX_08620"
     CDS             complement(2347..>2789)
                     /locus_tag="ACZPAX_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HIR1114000.1"
                     /translation="SQPSSGETVEGGTTVILYVSRSEVAKESQVPSLTGKTIEEARNE
                     VKGLNLSIRTVEQPSEQPEGTVLSQSPDAGTNVRKSSVITLVVSTGIPEAAVEPETPS
                     GGDGGIQESTWWSADGSHQITQHTDGSMWNENGQQCDAQGNPIA"
BASE COUNT          512 a          858 c          857 g          562 t
ORIGIN      
        1 cacaaaccca aatagttccc aacggggcaa aactgccggt gatgtccaga acgctctccc
       61 ctgcaccggg cagtgcggcg acggtctcgg cggtcagggt cttttcctcg gtcacggcgc
      121 actgcaggcc ctgcatcgtg cgggtgtact gctcaatgcc gcagtcggcc agcgaggtca
      181 gcagctccag ctctttgcgc acggctacgg cggcacacag gatgtacgcc ccgcgcaggt
      241 cgatacggtg ctcgctgaca gcgcggcagt tacagtattc cggttcgccc cagacctgcg
      301 ccggaccctc ggcgtactgc ccggcgggca gctcgaccga tttttcaaag gcaaatttct
      361 gttcggtgcg caaaaggcgc gtgccaggtt cctcgctctg gtaaaagacc gtgcagcgca
      421 ggtagccctc cacctgccag cgcccgcccg ccacccggtt ttgcagcacc accggcgcaa
      481 tcagacactt gacgatcttg aatactgcgg gcagatagtc gggtatcaaa atttcggttt
      541 cgacaggaag ttccagccgc gtttcccagc gaccgccata ggatgctatg gtgtctttga
      601 acaccttgag ttccatattg cttgcccctt tccggttatg ttctggggca ttatatgcgg
      661 cggggcaaaa aaatagcccc tgcccgggta cgggcagggg aatttttcaa acttcagacc
      721 ttgaaggctg cgcgcaggag ccagcggaag gcgcgcgggc agcggaccac gaccacatgc
      781 atggcataca cctcactttc catgtctgtg tacgccatac tatgcgggcg gcgggtcagg
      841 tgttacgcat cgtcgcgagt cagcaccaca acgccggtgc cgccgctgca ttgcaggtgg
      901 gcggtctcgg cgacaaattc attgctgacg cccaaaggat aatcggcggt cagcttcgcg
      961 ttttccagcg ggtagaacgc accgcggatg gcaagcccgg tgagctggcc ttccagaggg
     1021 aacaccgaga cgtacttcca gtcgctgcgg tgcagctcca tcggggtgtt cggcacaagg
     1081 aaatgcagct cactccgctc gttcgcaagc aggaccttca cgccgttttt ggcaaggtac
     1141 agcccggtgg caaggtttgc cagcgtatgt tccagccggg caccgcccag ggcacccagc
     1201 agggtgatct cgtcatagcc atgttccagc agccagcggg cagcgtactg ggtgtcggtg
     1261 tcgtccttca catggggcag cacgatcgtc tcgtgctcgg ccctggggcg cggggcagaa
     1321 tcaaaatcgc caacgatcag atcaggccgc aggtcaagcc gctcggcgtt gcggtagccc
     1381 gcatcgcagg ctacaatgaa atccccaggg cgcacatagc ggcccacggc ggtggatacc
     1441 ggcacggcgg aaaggataat ggcacgcttg ctcatttttc ccactccttt cgtgcgctgg
     1501 cttcttcgta catggcaagg tagctggcat agcgtgtttt gctgatggtc cccgagtcca
     1561 cggcctcacg cacggcgcag ccggtctcgc tgcggtggga gcagccggtg aaccggcact
     1621 ggccgaagta gggcgcaaat tccgggaagg tatgctggat atcatccttg gggatgcggc
     1681 agagcttttg cgcttccagg ctggcaaagc ccggtgtgtc ggccagacgg ccgccgcaaa
     1741 tctcaaaaat ctccacctcg cgggtcgtgt gacggccacg gcccagcttt tggctgatag
     1801 acccggtctc ccgcttcagc tccggtgcca gtgtgtttaa cagtgtggat ttgcccacgc
     1861 cggagttgcc gcagaaagcg cacaaatgcc cctggatata cccgcgtatc tcgtccagtc
     1921 cttcgccggt ttcgtagttc agctgtatca gcgggatgcc gctgcctgtg taggcggtgc
     1981 gcagcatatc cgcagcggcc aagtcggcct tggtcacgac cagcaccggc tgcacatcct
     2041 tgtagatggc ggcggcggcc agctcgtcca acaccaaggt gctgggcaca ggctgggtcg
     2101 tgctggtgac gatgaacaaa atatccaggt tggcgatggg tggacggata aagacattct
     2161 tgcgcggtaa aatctcatca atcatcgtgc catcggcgga cagggccacg ttatcccccg
     2221 ccaccggcgt gatgccccgc ttgcggaaga taccgcgggc tttgcattcc acgatgccgt
     2281 ccggcgtttt tacatagtaa aagccgccga tgccttttgt aatataagcc atggttccct
     2341 cacttgtcag gcgatgggat tgccctgggc gtcgcactgc tggccgttct cgttccacat
     2401 cgagccatcg gtgtgctggg tgatctggtg gctgccgtcg gcactccacc aggtgctctc
     2461 ctggatgccg ccgtcaccac cggaaggcgt ttccggctcg accgcggcct cggggatacc
     2521 ggtggagacg accagcgtga taacgctgga cttgcgcaca tttgtaccgg catcgggaga
     2581 ctggctcaga accgtgccct cgggctgctc gctgggctgc tccaccgtgc ggatggacag
     2641 attcagaccc ttgacctcgt tgcgggcttc ctcaatggtc ttgccggtca ggctcggaac
     2701 ctggctctcc ttggcgacct cggaacggct gacatacagg atgaccgtgg tgccgccctc
     2761 gaccgtctca ccggaggaag gctgggagt
//
LOCUS       DBMDSQ010000259         2777 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_8083_length_2777_cov_2.326607, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000259 DBMDSQ010000000
VERSION     DBMDSQ010000259.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 2777)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2777)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2777
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8083_length_2777_cov_2.326607"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..610
                     /locus_tag="ACZPAX_08625"
     CDS             <1..610
                     /locus_tag="ACZPAX_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase-like domain-containing protein"
                     /protein_id="HIR1114001.1"
                     /translation="RYILEDDYDSEFRFDTRPLPSLQGMAGPDGPVVDLTTFSKSLAP
                     GIRIACMVLPQSLLARYRRDFATYANTVSRFEQQTLCEFMAGGYFTRHLARMRLAYKR
                     RMEAFAAALRAALPGVELDGVHSGLHFLLTLPGAGGERAMVAAAAQQGVRLRGLSEYY
                     LARPELCREDTVVAGYSALREEDVERVAQALARAWLCAPSDG"
     gene            complement(683..1939)
                     /gene="glyA"
                     /locus_tag="ACZPAX_08630"
     CDS             complement(683..1939)
                     /gene="glyA"
                     /locus_tag="ACZPAX_08630"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005926763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HIR1114002.1"
                     /translation="MYKDIIDTIGYVESYDPELGAAMNRELGRQRQNIELIASENIVS
                     PAVMAAMGSVLTNKYAEGYPGHRYYGGCQYVDQVEQIAIDRACKLFGAKYANVQPHSG
                     AQANLAVYFALLNLGDTVMGMDLSQGGHLTHGSPVNMSGKNYNFVSYGVSAEDGRIDY
                     AALAKQVAKVRPKLIVAGASAYPRAIDFEKLAEIAHGYGAMLMVDMAHIAGLVAGGQH
                     QNPVPYADVVTTTTHKTLRGPRGGLILTNNEYLIKRINSAIFPGTQGGPLEHVIAAKA
                     VCFGEALKPEFKEYARKIVENAKALEAELTARGVKLVSGGTDNHLLLIDLTDEDCTGK
                     ELEHNLDEVHITANKNTVPGEKRSPFVTSGVRIGTPAVTTRGMGVDEMKVIADCIADC
                     IFDFAGKKDDIAKRVADLTAKFPLYE"
     gene            complement(2063..2650)
                     /gene="hisB"
                     /locus_tag="ACZPAX_08635"
     CDS             complement(2063..2650)
                     /gene="hisB"
                     /locus_tag="ACZPAX_08635"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007045561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HIR1114003.1"
                     /translation="MARTATVTRNTRETQITLTIDLDGTGKADLHTGIGFFDHMLDGF
                     ARHGLFDLSVTCHGDLDVDCHHTIEDIGIALGTAIKEVLGDKAGLVRYGSCLLPMDET
                     LALCAVDLGGRPYFVYDAAFAGQSCGGMDTQMAREFFYAVSYSAMMNLHLKVLYGEND
                     HHKLEAMFKAFAKALDAATRKDARIDGVLSTKGTL"
BASE COUNT          494 a          853 c          871 g          559 t
ORIGIN      
        1 gcggtatata ttggaggacg actacgactc ggagtttcgc tttgacacgc ggcctttgcc
       61 cagcttgcag ggcatggcag gcccggacgg cccggtcgtc gacctgacga ccttttccaa
      121 aagccttgcg ccgggcatcc gcattgcctg tatggtgctg ccccaaagcc tgttggcccg
      181 ctaccgccgc gattttgcga cctatgcgaa cacggtcagt cggtttgagc agcagactct
      241 ttgcgagttc atggcgggcg ggtattttac ccggcacctg gcccggatgc gccttgccta
      301 caagcgccgt atggaggcat tcgcggcggc gctgcgcgcg gccctgcccg gcgtcgagct
      361 ggacggcgta cacagcggcc tgcactttct gctgacgctg cccggcgctg gcggcgagcg
      421 ggccatggtg gccgccgccg cgcagcaggg cgtgcggctg cggggattga gtgagtatta
      481 cctggcccgc cccgagctgt gccgggagga tacggttgtg gcggggtatt ccgcgctgcg
      541 agaggaagat gtggaaaggg ttgcccaggc gctggcaagg gcgtggcttt gcgcaccaag
      601 cgatgggtaa agccccaacc acccacaaaa aaatcccgcc ggggcgtaca cctcggcggg
      661 acggtttatt tattgctcaa aatcactcat acagcgggaa cttcgcagtc aaatcggcaa
      721 cgcgcttggc aatatcgtcc ttcttgcctg caaagtcaaa gatgcagtct gcgatgcagt
      781 ccgcaatcac cttcatctcg tcaacgccca tgccgcgggt cgtcacggcg ggggtgccaa
      841 tgcgcacgcc gctggtcaca aaggggctgc gcttctcacc ggggacggtg ttcttgttgg
      901 cggtgatgtg tacctcgtca aggttatgct ccagctcctt accggtgcag tcctcatcgg
      961 tcaggtcaat cagcagcaga tggttgtcgg tgccgccgga gacaagcttc acgccgcggg
     1021 cggtcagctc ggcctccagt gccttcgcat tttcaacgat cttgcgggca tactccttga
     1081 actcgggctt cagcgcctcg ccaaagcaga cggccttcgc agcgatgacg tgttccagcg
     1141 ggccgccctg ggtgccgggg aagatggccg agttgatgcg cttgatgaga tactcgttgt
     1201 tggtcaggat caggccgccg cgggggccgc gcagcgtctt gtgggtcgtg gtggtcacaa
     1261 cgtcggcgta ggggaccggg ttctggtgct ggccgcctgc taccagaccg gcaatgtggg
     1321 ccatatccac catcagcatc gcgccgtagc cgtgggcgat ctcggccagc ttctcaaagt
     1381 cgatggcgcg ggggtaggcg gacgcaccgg ccacgatcag cttggggcgg accttcgcca
     1441 cctgcttggc caacgcggcg tagtcgatgc ggccatcctc ggccgagaca ccgtaggaga
     1501 cgaagttgta gttcttgccg ctcatgttca cgggggagcc gtgggtcagg tggccgccct
     1561 gggacagatc catgcccatg acagtgtcac cgaggttcag cagcgcaaag taaaccgcca
     1621 ggttcgcctg tgcgccggag tggggctgca cgttggcgta cttggcaccg aacagcttgc
     1681 aggcgcggtc aatagcgatc tgttccacct ggtccacgta ctggcagccg ccgtagtagc
     1741 ggtggccggg gtagccttcg gcgtatttgt tggtcagcac gctgcccatg gccgccatca
     1801 cggcagggga gacgatgttc tcactggcaa tcagctcaat gttctggcgc tggcggccca
     1861 actcgcggtt catggccgcg cccagctcgg ggtcatagct ttccacatag ccgatggtgt
     1921 caatgatgtc cttgtacata ctgctttccc tcctattata tctgccgacg gcggaaaacg
     1981 ggcacgggcg gatatggaat gcgcccctac cgtaggggcg cattctatat gcgcccgtcg
     2041 tttccatcgc cgaacatttc ccttacagcg tcccctttgt cgataacacg ccgtcgatcc
     2101 gcgcatcctt gcgggtggca gcgtccaggg ctttggcaaa ggctttgaac atggcctcca
     2161 acttgtggtg gtcgttctcg ccgtacagca ctttcaaatg cagattcatc atggcgctgt
     2221 acgataccgc gtagaaaaac tcccgcgcca tctgggtgtc cataccgccg cagctctgcc
     2281 cggcaaaggc agcgtcgtac acaaagtagg gccgcccgcc cagatccacg gcgcagagcg
     2341 ccagcgtctc atccatgggc aaaaggcagc tgccgtaccg cacaagacct gccttgtcgc
     2401 cgaggacctc cttgatggcg gtgcccagcg caatgccgat atcctcgatc gtgtggtggc
     2461 agtcgacgtc cagatcgccg tggcaggtca cgctcaaatc aaacaggccg tggcgggcaa
     2521 agccgtccag catatggtca aaaaagccga tgccggtgtg caggtctgcc ttgccggtgc
     2581 cgtccaagtc gatggtcagc gtgatctgcg tctcccgcgt gttgcgggtc acagtcgcgg
     2641 tgcgtgccat gaaaaattcc ccctctttag tgcgttaaac taaggcaaca ccgcacaatt
     2701 cccgcctgac agcttaagca ccgctccggc ggctgcggca cggcatctgc gttgccaaaa
     2761 tgctcgataa gacatcc
//
LOCUS       DBMDSQ010000260         2767 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_8117_length_2767_cov_1.735184, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000260 DBMDSQ010000000
VERSION     DBMDSQ010000260.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 2767)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2767)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2767
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8117_length_2767_cov_1.735184"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(123..506)
                     /locus_tag="ACZPAX_08640"
     CDS             complement(123..506)
                     /locus_tag="ACZPAX_08640"
                     /inference="COORDINATES: protein motif:HMM:NF015898.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HIR1114004.1"
                     /translation="MPKIKRLPDAELEIMNALWDAAAPLTAAELETALPGPPRARTTL
                     LTLLARLEEKGCVTREKQGRGYLYTATLTRSEYLPAESKSAWHRLFGGSPRSFVAALA
                     ETDTLTDADIDELTAYLEELKKERK"
     gene            complement(519..2576)
                     /locus_tag="ACZPAX_08645"
     CDS             complement(519..2576)
                     /locus_tag="ACZPAX_08645"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017390.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HIR1114005.1"
                     /translation="MFLPLFIAFLVQGLLLGLVVLALLAAAPKLQKRYGPRWLCRLWV
                     VLAVLFLVPVRALLPQAPAAVTVSAQSFYTQMTALTEETTTKPAANVPYRTTTADDNT
                     MHYIVPRAELQTGRRTVLERLAIDTNWGNLAALAWELGLTAVALYQFGGYAVWRIRVG
                     RNAQPVAKSWRDALPQGQCPQMQATPLVRSPMVAGALHPVLLVPAGEALKGAECMLAH
                     ELTHIKRHDVAKKLLLTLVCILHWYNPAVWMLAARAGRDIEEACDAETLHGRDAAYRT
                     AYADALMTAVRRSRGPALTSGFALNKRQFKQRLAALWDTAPKRRGRTLLAALALTACC
                     AGGLVACQVADTPTQETPEPAPTATPSTEEALAEIKDINRQLPTEQQLTATRRVDSYF
                     WDECLPTLWGYKFNGGMTSLVEDPAAESAENENLAAILQEPRADGTTWQDFLNAGKGT
                     FAKVNFEAELEPEKLFLGPQYGEGDFYIYIAVPYDESEPCELVSGWQQNNTGETAHFG
                     TIDPARELRLTNRQYRTLVHQCNALAQAGARDFGTPADWPQAELETYLYYRNQDFHTF
                     ADALLMLNIDFTGGRDWNSLPYFLTYDELNALDFTAPKTDAIPDYVVAEGDDCGVWAF
                     TRDGDYLTATWAGVQEYIFYVYNGDTAARTVIVGPNGEQTAPITEMPYDARTVIAGGH
                     SLD"
BASE COUNT          483 a          793 c          924 g          567 t
ORIGIN      
        1 tgcagatcac cgggcatagc gcgtcacctc acttgcagat actatgggac atattattat
       61 actacaagtt gagggagaat tgcaagtagg ggcgagcagt gctcacccct acaaatttta
      121 cgctattttc tctctttttt cagttcctcc agatacgccg tcagctcgtc tatgtcggca
      181 tcggtcaggg tatcggtttc tgccagggcg gcgacgaagc tgcgggggct gccgccgaac
      241 aggcggtgcc acgcgctttt gctctcggcg ggcaggtatt cactgcgggt cagggttgcg
      301 gtgtaaaggt accccctccc ctgcttttcg cgggtgacac agcccttttc ttccagccgg
      361 gccagcaggg tcaacagcgt ggtgcgggcg cggggcgggc cgggcagggc ggtttccaat
      421 tcggcggcgg tcaggggcgc ggcggcatcc cacaaagcgt tcatgatttc cagctctgca
      481 tcgggcaggc gtttgatttt tggcatgggc gtctcctttt aatccaagct gtggccgccg
      541 gcaatgaccg tgcgggcgtc gtacggcatc tcggtaatag gtgcagtctg ctctccgttc
      601 gggccgacaa tcaccgtgcg ggcggcagtg tcgccgttgt agacatagaa gatgtattcc
      661 tgcacgcccg cccacgtggc ggtcaggtag tcgccgtcgc gggtgaacgc ccatacgccg
      721 cagtcgtcgc cctcggccac aacgtagtcg gggatggcgt cggtcttggg cgcggtgaaa
      781 tccagcgcat tcagctcatc gtaggtcaaa aagtacggca ggctgttcca atctcgaccc
      841 ccggtaaagt cgatgttcag catcagcagg gcatcggcaa aggtgtggaa atcctgattg
      901 cggtaatata ggtaggtttc cagctcggcc tgcggccagt cggcgggggt gccaaaatcc
      961 cgtgcgcccg cctgggccag cgcgttgcat tggtgtacca gggtgcggta ctggcggttg
     1021 gttagccgca attcccgcgc ggggtcgatc gtgccgaagt gggccgtctc gccggtgttg
     1081 ttctgctgcc agccactgac aagctcgcag ggttcgctct cgtcataggg cacggcgata
     1141 taaatataaa aatcaccctc gccatactgt gggcccaaga aaagtttttc cggctcgagt
     1201 tctgcttcaa agttcacctt ggcgaacgtg cctttgcctg cgttcagaaa gtcctgccag
     1261 gtcgtgccgt ccgcgcgggg ttcctgcaaa atggcggcca gattttcgtt ttcggcactt
     1321 tcggcggcgg ggtcctcgac cagcgaggtc ataccgccgt tgaacttgta gccccacagg
     1381 gtcggcaggc actcgtccca gaagtaactg tccacgcgcc gtgtggcggt gagctgctgc
     1441 tccgtgggca gctgacggtt gatgtccttg atctccgcaa gggcttcttc tgtcgagggc
     1501 gtcgcagtgg gcgcgggttc gggtgtctcc tgtgttgggg tatcggcgac ttggcaggct
     1561 accaggccgc ccgcgcagca ggcggtcagg gccagtgcgg ccagcaaagt gcggccccgg
     1621 cgtttggggg cagtgtccca tagcgcggca aggcgctgtt tgaactggcg cttgttcagc
     1681 gcaaaaccgc tggtcagagc cgggccgcgg ctgcggcgca cggcggtcat cagggcatcg
     1741 gcgtaggccg tacggtaagc agcgtcgcgg ccgtgcaggg tctcggcgtc gcaggcttcc
     1801 tcgatatcac ggcccgcgcg ggcggccagc atccagacag ccgggttgta ccagtgcagg
     1861 atgcacacca gcgtgagcag cagctttttc gcgacgtcat gtcgttttat gtgggtcagt
     1921 tcatgggcca gcatacattc cgcacccttg agggcttcgc ccgcagggac gagcaggacg
     1981 ggatgcagcg cccccgccac catggggctg cgcaccagcg gcgtggcctg catttgcggg
     2041 cattggccct gcggcagggc gtcacgccag cttttagcca ccggctgcgc gttgcggccc
     2101 acgcgaatac gccagacggc atagccgccg aactggtaca gcgccacggc ggtaaggcca
     2161 agctcccacg cgagggccgc caggttgccc cagtttgtat cgatggcaag acgttccagc
     2221 acggtgcgcc gtccggtttg cagttccgcg cggggcacga tgtaatgcat cgtgttgtcg
     2281 tcggcggtgg tcgtgcggta gggtacgttg gcggcgggct tcgtggtggt ttcctccgtc
     2341 agggcggtca tctgggtata aaacgactga gcgctgaccg tcaccgccgc cggggcctgg
     2401 ggcaaaagtg cccgaaccgg cacgaggaac agcaccgcca gcaccaccca caggcggcag
     2461 agccaccgcg ggccataccg tttttgcagc ttgggggctg cagccagcag ggccagcacc
     2521 acaaggccca gcagcaggcc ttgcaccaga aaggcgatga aaagcggcag gaacatggtg
     2581 ggcctccttt tgattctaca gttgtagaag gttggatttg attttattct acaagtgtag
     2641 aattgtattg tcaagtggca tgggtgacga attattgggg gagtttttgg ggaaagtgcg
     2701 cagtggtggc atggcacacc ctggaagaag cgctggacgg cgaaggcaag accgataagg
     2761 ttacgac
//
LOCUS       DBMDSQ010000261         2765 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_8125_length_2765_cov_1.750751, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000261 DBMDSQ010000000
VERSION     DBMDSQ010000261.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 2765)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2765)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2765
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8125_length_2765_cov_1.750751"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..680)
                     /locus_tag="ACZPAX_08650"
     CDS             complement(<1..680)
                     /locus_tag="ACZPAX_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HIR1114006.1"
                     /translation="MSKEFRTSVGGQALMEGIMMRGPEKICCAVRKPDGSIDLTYDTV
                     TTRWYNKVPLVRGVCNMAENLYKGYKYLMHAADVAMEGETEPAESKLDKWFEEHATPA
                     VQNAMMICAAFVGVALALFLFTFLPTFLTGLVMRAAPLGRWPRVILEGVLKMVIFLGY
                     MFLCTRFKELHRVFEYHGAEHKTIACYEAGLPLTVENIRKQSRFHPRCGTSFMILVII
                     ISIFLYAVL"
     gene            complement(901..2349)
                     /locus_tag="ACZPAX_08655"
     CDS             complement(901..2349)
                     /locus_tag="ACZPAX_08655"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR1114007.1"
                     /translation="MQPSSIIRRWIRGSLLITVLVLAIAEGLFLYYSYNDLYGGVERA
                     MESRFSTVVGRLQATGTAGNTATTAESRGQALRRVVEQFDEKDKFEFMLLDGQGVILA
                     TSSGTMSRDLTDGTDYGRALTSANGMGAAIFTTPNGERVMAVTMLVPYAAGSIAAMRM
                     VTSLSLVDARWWRTIAICIGLGVLVLTFTVWSGLFFVRSIVRPLGEVEATATKIAKGD
                     MKVRLPDTRYNDEIGRLCKTINQMAEDLAETERLKNEFISLVSHELRTPLTSIKGWVE
                     TIENIDDPTNENYRRGLSVIGTETDRLYTMVEELLDFSRLQNGIKMNCQVLDFVAEAT
                     DAALFVEARIRQEGMQLVYSEPEEPYPVWADPARLRQVFVNLFDNAIKYSEPSGTIFL
                     ELSRTPVTVTASIRDQGRGIAADDLEKVKQKFFKAKNSVRGSGIGLAVVDEIVQTLGG
                     RFDIDSTLGQGTTVTVTLPIYHPGQEHLHEKI"
     gene            complement(2577..>2765)
                     /locus_tag="ACZPAX_08660"
     CDS             complement(2577..>2765)
                     /locus_tag="ACZPAX_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HIR1114008.1"
                     /translation="FRNPATALVREKILEGVWGKNYFGDVKIVDVNIRRLRMKIEDEP
                     SSPQHIMTVWGYGYRWNA"
BASE COUNT          525 a          824 c          863 g          553 t
ORIGIN      
        1 agcaccgcat acaaaaagat gctgataatg atgacgagaa tcataaagct ggtgccgcag
       61 cgggggtgga agcggctctg tttgcggatg ttttccaccg tcaggggcag cccggcctcg
      121 taacaggcga tggtcttatg ctcggcaccg tggtactcaa agacgcggtg cagctccttg
      181 aagcgggtgc agaggaacat atacccaagg aagatgacca ttttcagtac gccttccagg
      241 atgacgcgcg gccagcggcc cagcggcgcg gcccgcatga cgaggccagt caaaaacgtg
      301 ggcaggaagg tgaacaggaa cagcgccagc gccacgccga cgaaagccgc gcagatcatc
      361 atggcattct gcacggcggg ggtggcgtgt tcctcgaacc atttgtccag cttggactcg
      421 gcgggttcgg tctccccctc catggcgacg tcggcagcgt gcatcaggta tttgtaaccc
      481 ttatagaggt tttcggccat gttgcagacg ccgcgcacca gcgggacttt attgtaccag
      541 cgggtcgtga cggtgtcgta ggtcagatca atgctgccgt ccggcttgcg caccgcgcag
      601 cagattttct ccggtccgcg catcatgatg ccctccatca gggcttgccc gccgacggag
      661 gtgcgaaatt ctttggacat aagtgctctc ctttgtttct ttgccttctc ccttgggact
      721 gcgcccgcag gcgcgtgtcg gagcgcaacc gccgaaggtg gctcttagcg cggaggctga
      781 aggtggcccc gcagggccgg atgaggggcg ggtgtgccgt cattgcccgg taaagggtgc
      841 tgcggcaagg tcgcccctca tcagtccgct tcgcggacag cttccccctc gggggaggcc
      901 ttaaatcttt tcatgcagat gttcttgccc ggggtggtag atgggcaggg tgacggtgac
      961 ggtcgtgccc tgacccaggg tcgagtcgat atcgaatcgg ccgcccaacg tctggacgat
     1021 ctcatcaacg acggccaggc cgatgcccga gccgcgcacc gagttctttg ccttgaagaa
     1081 tttctgcttg accttctcga ggtcatctgc cgcgatgccc cggccctggt cgcggatgga
     1141 cgccgtgacc gtgaccgggg tgcggctcaa ttccagaaaa atcgtaccgc tcggttcgga
     1201 atattttatc gcattatcga agagattcac aaacacctgc cgcaggcgtg cgggatcggc
     1261 ccagaccggg tagggttcct ccggctcgga atagacgagc tgcatcccct cctggcggat
     1321 acgtgcctcg acaaaaagcg ctgcgtcggt ggcctcggcc acaaaatcca gcacctgaca
     1381 gttcattttg atgccgtttt gcaggcggga gaagtcgagc agctcctcca ccatcgtgta
     1441 gagacggtcg gtctcggtgc cgatgaccga caggccgcgg cggtaatttt cattcgttgg
     1501 gtcgtcgata ttttcaatcg tctcgaccca gcctttaatg gaagtcagcg gggtgcgcag
     1561 ctcatggctg accaacgaga taaactcgtt tttcagccgc tcggtctcgg ccaggtcctc
     1621 ggccatttgg ttgatggtct tgcacaggcg gccaatttca tcgttgtatc gtgtatccgg
     1681 aaggcggact ttcatgtcac ccttggcaat tttcgttgcg gtggcttcca cctcacccag
     1741 ggggcgcacg atggagcgca caaaaaacag gccgctccag acggtaaagg tcagcaccag
     1801 cacgcccagg ccaatgcaga tggcaatggt gcgccaccac cgcgcgtcca ccagcgagag
     1861 gcttgtcacc atccgcatgg cggcgatgct gcccgccgcg tagggcacga gcatcgtcac
     1921 ggccatgacg cgctcgccgt tgggcgttgt gaagatggcc gcccccatgc cgttggccga
     1981 ggtaagcgcc cggccatagt cggtgccgtc ggtcaagtcg cggctcatgg tgccgctgct
     2041 ggtcgccaga atgacgccct ggccgtccag cagcatgaac tcgaacttgt ccttctcgtc
     2101 gaactgctca accacgcggc gcaaggcctg gccgcggctt tcggcggtgg tcgcagtatt
     2161 ccccgccgtg ccggtggcct gcaagcggcc cacgacggtg gaaaaacggc tttccatcgc
     2221 acgttccacc ccgccgtaga ggtcattgta gctgtaatac aggaacagcc cctcggcaat
     2281 cgccagcacc agcacggtga tgagcaggct gccgcgtatc cagcggcgga tgatactgct
     2341 aggctgcatg agggggacct cctttctggg gggatttttt gggggctgaa gtgtcaggtc
     2401 gtgataaagg caaggtgcgg cggggtgagg gcaccccgcc ctacggtgct aatgcaaagg
     2461 ggatgtgacg gcaaggcagc ggggcgtcga ggacgccgcc ccctacgaca aaggcaagga
     2521 ggatatgcgg cggccagagg actggccgcc ctacgaaagc agccgtcaaa cccttattac
     2581 gcgttccacc ggtacccgta cccccacacg gtcatgatat gctgggggct gctgggctcg
     2641 tcctcgattt tcatgcgcag gcggcggatg ttcacatcga caatttttac gtcgccaaaa
     2701 tagttcttgc cccagacacc ctccaggatt ttttcacgta ccaacgccgt agccgggttg
     2761 cggaa
//
LOCUS       DBMDSQ010000262         2758 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_8149_length_2758_cov_1.931125, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000262 DBMDSQ010000000
VERSION     DBMDSQ010000262.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 2758)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2758)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2758
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8149_length_2758_cov_1.931125"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..759
                     /gene="prmA"
                     /locus_tag="ACZPAX_08665"
     CDS             <1..759
                     /gene="prmA"
                     /locus_tag="ACZPAX_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HIR1114009.1"
                     /translation="LLDKPRDVVIVHMYLAPDENPAEVLPLFEERLQNSGINYQLNTT
                     GVEQEDWQNGWKKYYHAMDIGDRLAIVPGWEDHQTDRIKIIMDPGMAFGTGTHETTSL
                     CLETLDSLVKGGERVLDIGTGSGILAIAALKLGAAEAEGVDIDPMCVRTAGENAQRNG
                     VADHFKVLVGDLSDQASGQYNIITANIVAAAILSLAPHVPPLMAPGARFIASGIITER
                     RDEVLTGLQAAGLAPIEVKEKRGWVCIICEKKEA"
     gene            763..1491
                     /locus_tag="ACZPAX_08670"
     CDS             763..1491
                     /locus_tag="ACZPAX_08670"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005943737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HIR1114010.1"
                     /translation="MPHRYFTRELADGRAALTGSDAHHLADVMRAKLGEEVVLCGPDG
                     LEYLGTVTAIEPGRVEFSVTDGTTSKAEPDCAVTLFAGYPKAGKLEEVIRHSVELGVT
                     EVVPFFSRYCVAAPKKEDVKCERYNRIAAEAAKQAGRATLPHVAMPLPDFGAVCKAFA
                     DFDLVLFFYEGGGAPLREILHPSRYKRIAIVTGSEGGFSVEEAAAAKAAGAVTVGLGP
                     RILRCETAPLAALTATMLLTGNLE"
     gene            complement(1666..2589)
                     /locus_tag="ACZPAX_08675"
     CDS             complement(1666..2589)
                     /locus_tag="ACZPAX_08675"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007492150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000701 - purine-specific mismatch base
                     pair DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HIR1114011.1"
                     /translation="MLQQTRVAAAIPYYERFIAALPNPAALAACEPDALRKLWQGLGY
                     YNRVNNMQKAARIVCEQYGGDLPADYDALRSLPGIGDYTAGAVASIAFGIPAPAVDGN
                     VLRVFARLYNDDADVMNPATKRLFTERVLAQMPKATPGPYNEALMELGALVCVPGTPR
                     CGECPLADLCQGYAAGRAAELPVKPAPKAKSKVPVTVALIESERGLLLQRRPAHGLLA
                     GLWQPAAWEKALTRDELCAALAKLGVQAALAAPLPPAKHVFTHKVWELGGWKGTAKAC
                     ELPDGYVWAAAEDLAEVYPVPNAFGAYVKAK"
BASE COUNT          524 a          876 c          879 g          479 t
ORIGIN      
        1 ctgctggaca agccccgcga cgtcgtcatc gtacatatgt accttgcacc cgatgaaaac
       61 cccgccgagg ttctgcccct gtttgaggaa cgtctgcaga acagcggcat caactaccag
      121 ctcaacacca ccggcgtcga gcaggaggac tggcagaacg gctggaaaaa atactaccac
      181 gcgatggata ttggcgaccg ccttgccatc gtgcccgggt gggaggatca ccagaccgac
      241 cgcatcaaga tcatcatgga cccgggcatg gccttcggca ccggcacgca cgagacgact
      301 tcgctctgcc tggaaacgct ggacagcctg gtcaagggcg gcgagcgggt gctggatatc
      361 ggtaccggct ccggcattct ggccattgcc gcactgaagc tcggcgcagc cgaggccgag
      421 ggcgtggaca tcgacccgat gtgcgtgcgc actgcgggtg agaacgccca gcgcaacggc
      481 gtggccgacc actttaaagt cctcgtcggc gatctgtccg accaggccag cgggcagtac
      541 aacatcatca cggccaacat cgtggcggcg gccatcctgt cgctggcccc ccacgtgccg
      601 ccgctgatgg ccccgggcgc acgtttcatc gccagcggca tcatcaccga gcgcagggat
      661 gaagtgctca ccgggctgca agccgcaggt cttgcgccca tcgaggtcaa ggaaaagcgc
      721 ggctgggtct gcatcatctg cgaaaagaaa gaggcgtaaa gtatgccgca tcgttatttt
      781 acccgggagt tggccgatgg ccgcgccgcg ctgaccggca gcgatgccca ccacctggcc
      841 gacgtcatgc gtgccaaact gggtgaggaa gtcgtcctct gcggcccgga cgggctggaa
      901 tatctcggca cggtcacggc cattgagccg gggcgggtgg agttttccgt caccgacggt
      961 acgaccagca aggccgagcc ggactgcgcc gtaacgctgt ttgcgggcta ccccaaggcg
     1021 ggcaaattag aagaagtcat ccgccacagc gtcgagctgg gcgtgactga agttgtaccg
     1081 tttttcagtc gatactgcgt agcagcgcca aaaaaagagg atgtgaagtg cgagcggtac
     1141 aaccgcattg ccgccgaagc cgccaagcag gcgggccgcg caacgctgcc ccatgtggcg
     1201 atgccgctgc cggattttgg tgcggtgtgc aaggcatttg cggattttga ccttgtgctg
     1261 ttcttctacg agggcggcgg tgcgccgctg cgggagatcc tgcaccccag ccgatacaag
     1321 cgcattgcca tcgtcaccgg gagcgagggc ggtttttccg tcgaggaggc cgccgctgcg
     1381 aaagctgccg gggccgtgac ggtaggctta ggcccgcgca tcctgcgatg cgagaccgcg
     1441 cccctggccg cgctgacagc gacgatgctg ctgacgggga atttggagta accacaaacc
     1501 atagtatgta aaaagaaaga ggagcgccac ccgcatgtgg gtagcgctcc tctttgtgtt
     1561 attattgccc ttgtaggggc cgggcatgcc cggcccgcag gtaaaaggct aacggacact
     1621 aatgggaaac ccgcgggcgg catatatgcc gcccctacag ctctactatt tcgccttcac
     1681 atacgcccca aacgcattcg gcacggggta tacctcggcc aagtcctcgg cggctgccca
     1741 gacatagccg tcgggcagct cacacgcttt cgccgtgcct ttccacccgc caagttccca
     1801 aaccttgtgc gtaaatacgt gctttgcggg cggcagcggc gcggccagcg cggcctgcac
     1861 gcccagtttc gccagcgcag cgcacagttc gtcccttgtc agagcctttt cccatgcggc
     1921 gggctgccac aatccggcca gcaggccgtg ggcgggccgc cgctgtaata gtaacccccg
     1981 ctcgctttca atcagcgcca ccgtcactgg cactttgctc ttggccttgg gcgcgggctt
     2041 cacgggcagt tccgccgcgc ggcccgccgc atacccctgg cacaggtcgg ccagcgggca
     2101 ttccccgcac cggggcgtgc cgggcacgca gaccagcgcc cccagttcca tcagggcctc
     2161 gttgtagggg ccgggggtgg ctttgggcat ctgcgccaaa acccgctctg taaacagccg
     2221 cttcgtcgcc ggattcatca cgtcggcgtc gtcgttgtac agccgcgcaa agacgcgcag
     2281 gacgttcccg tccaccgcag gggcggggat gccaaaggca atgctcgcca ccgcgcccgc
     2341 cgtgtagtcg ccgatgcccg gcaggctgcg cagggcgtcg tagtcagcgg gcaggtctcc
     2401 gccgtactgc tcacagacga tgcgggcggc tttctgcata ttgttgacgc ggttgtaata
     2461 gccaaggccc tgccacagct tgcgcaaggc gtccggctca caggccgcca gggcggcagg
     2521 gttcggcaaa gctgcgataa aacgttcata atagggtatc gccgctgcca cgcgggtctg
     2581 ctgcagcata atttcgctga cccagatgtg gtacgcagaa ggctcctgcc ggaaaggcag
     2641 gagcctcttg tttgcgttaa accattgcag cagcggtgca gcaatgggcc gcattaaaaa
     2701 ccaccgcagg tacgggggaa cgggatgaca tcgcggatgt tggggatgcc ggtgacat
//
LOCUS       DBMDSQ010000263         2756 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_8155_length_2756_cov_1.774011, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000263 DBMDSQ010000000
VERSION     DBMDSQ010000263.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 2756)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2756)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2756
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8155_length_2756_cov_1.774011"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..60)
                     /locus_tag="ACZPAX_08680"
     CDS             complement(<1..60)
                     /locus_tag="ACZPAX_08680"
                     /inference="COORDINATES: protein motif:HMM:NF014542.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HIR1114012.1"
                     /translation="MVQVDLITGFLGSGKTTFLR"
     gene            complement(64..1035)
                     /locus_tag="ACZPAX_08685"
     CDS             complement(64..1035)
                     /locus_tag="ACZPAX_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015566102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR1114013.1"
                     /translation="MQNYRWATLGCGVIANELAQALASRGQTLYSVANRTHAKAEAFA
                     EKYGIEKVYDQIDDVFVDPDVDIIYISTPHNTHIQYLRKALAAGKHVLCEKSITLNSD
                     ELDEAIRLAEEHHVVLAEAMTLYHMPIYRKLQEKLDTGALGELRLIQMNFGSYKPYDM
                     SNRFFNRNLAGGALLDIGVYALSFVRWFMTSAPDEVVSQVRFAPTGVDEQAGILLKNK
                     EQEMASVILSLHAKQPKRGTVAFDKGYVEFYDYPRGQEAVITYTEDGSKETLRGGSTA
                     DALLYEVEDMERAIAGQGTMHLDYTRDVMRIMTDIRRQWGMTYPEEE"
     gene            complement(1453..2409)
                     /locus_tag="ACZPAX_08690"
     CDS             complement(1453..2409)
                     /locus_tag="ACZPAX_08690"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015564949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HIR1114014.1"
                     /translation="MKIQKVALLGAGAVGSYVIWGLSRCPGVQFGVIAEGSRAERLRQ
                     GCAVNGEILRPVVWTPQEAQGADLLIVALKYGALPGALDTICTAVTESTTVMSLMNGV
                     DSEERIAAAVGTEHLLYSLIKIASCHDGNCYRFDPEATIGIIYGELQEPCQSERVQAV
                     AELFAAAGLHARVTEHIREEMWSKFRLNVCNNLPQAILGAGVGCYQDSVHMKAISDGL
                     RAELETIAAAKGIDLRLVEASSRHGSAVPPTARYSTLQDLDAGRHTEIDMFSGALIRM
                     GKELGIPTPYNEYTYHMIKALEEKNDGLFDYDKANRRSTWAI"
     gene            complement(2484..2557)
                     /locus_tag="ACZPAX_08695"
     tRNA            complement(2484..2557)
                     /locus_tag="ACZPAX_08695"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2521..2523),aa:Val,seq:cac)
BASE COUNT          581 a          780 c          808 g          587 t
ORIGIN      
        1 acgcagaaaa gtcgtctttc cggaacccaa gaacccggtg atgagatcga cctgtaccat
       61 cgcttattcc tcctctgggt aggtcatgcc ccactggcgg cggatgtcgg tcatgatgcg
      121 catgacgtcg cgggtgtagt ccaggtgcat tgtgccctgc ccggcaatgg cgcgctccat
      181 gtcctcaacc tcgtagagca gggcgtcggc ggtgctgccg ccccgcagcg tctccttact
      241 gccgtcctcg gtgtaggtga tgacggcctc ctgcccgcgg gggtagtcgt agaattcgac
      301 atagcccttg tcaaaggcga ccgtgccgcg cttgggttgc ttggcgtgca gcgagagaat
      361 gaccgacgcc atctcctgct ccttgttttt caaaaggatg cccgcctgct cgtccacgcc
      421 cgtgggggca aaccgcacct gggagacgac ctcgtccggt gcggaggtca tgaaccaccg
      481 cacgaaggac agcgcgtaga cgccgatatc cagcagcgca ccgcccgcga gattgcggtt
      541 gaagaagcgg ttggacatat catagggctt gtaactgcca aaattcatct ggataagccg
      601 cagctcgccc aacgcgccgg tgtcgagctt ttcctgcaat ttgcggtaga tgggcatatg
      661 gtagagggtc attgcctcgg ccagcacgac gtggtgctcc tcggccaggc ggatggcctc
      721 gtccagctcg tcagagttca gggtgatgga tttctcgcac agaacgtgct tacccgcggc
      781 cagtgccttg cgcaggtact ggatgtgggt gttgtgcgga gtggaaatat agatgatatc
      841 cacgtccggg tcaacgaaca catcgtcgat ctggtcgtag actttttcaa tgccgtactt
      901 ctcggcgaag gcctcggctt tggcatgggt gcggttggcg acggaataca gtgtttgccc
      961 gcggctggcc agcgcctgcg ccagctcatt ggcgataacg ccgcagccca gggtggccca
     1021 acggtagttt tgcataaaca aaatctcctt tcccggttct gccaacagta tagcgcagtt
     1081 gcggggaaat gtccatcggc ccccaaaaga aaaagccgcc cggcatacac cgggcggcga
     1141 gaggtggggt tacagtgggg ctgtttagcc cagcagttgg ctgagcagca cggaggggcc
     1201 gacgcgatag ccaggtacag cacggtggca aggccgcgct tctgggcctc atccaggtag
     1261 ccttgtgggt aaaaatacag gcagttgcgc ccgctgtccc gcgccaggaa ctttactatc
     1321 aaaagaaaca gcgggggcag tataacagga tttgcaggga taacataggg gcctcccgtt
     1381 agcggtaaag agatttttgt gaagaatttt tatttattat agcaaatatt cgcaaagaaa
     1441 gaggcttgcc aatcagatag cccacgtaga tctacggttg gctttgtcgt aatcaaacag
     1501 cccgtcgttc ttttcttcca gcgccttgat catgtggtag gtatactcgt tgtagggcgt
     1561 ggggatgccg agctctttgc ccatgcggat cagcgcaccg gagaacatat cgatctcggt
     1621 gtgccgcccg gcgtccaggt cctgcaaggt cgaatagcgg gccgtcggcg ggacggcgct
     1681 gccgtggcgg gaagatgcct ccacaaggcg cagatcgatt cctttggcgg cggcaatcgt
     1741 ctccagttcg gcccgcagcc cgtcgctgat ggccttcatg tggacgctgt cctggtagca
     1801 gccgaccccg gcacccaaaa ttgcctgcgg gagattgttg cagacgttca gccggaactt
     1861 gctccacatt tcctcgcgga tatgctcggt gacgcgggcg tgcagcccgg cagcggcaaa
     1921 cagctctgcc acggcctgca cccgctcgct ctggcagggc tcttgcagtt caccgtaaat
     1981 gatgccgatg gtggcttccg ggtcaaagcg atagcagttg ccgtcatggc aggaggcaat
     2041 cttgatgagc gaatacaaca ggtgctccgt gccgaccgcg gcggcaatgc gttcctcgct
     2101 gtccacgccg ttcatcaagc tcatgaccgt ggtgctttcg gtgacagcgg tgcagatggt
     2161 gtccagtgca ccgggcagtg ccccatattt cagcgcgacg atcagcagat cagccccctg
     2221 tgcctcctgc ggcgtccaga cgacgggacg caagatttcg ccattgactg cgcagccctg
     2281 gcgcaggcgc tcggcgcggc tgccctcggc aatgacgccg aactgaacgc ccgggcagcg
     2341 ggaaagcccc caaatcacgt aggagccgac agccccggcc cccagcagtg caactttttg
     2401 aatcttcata gatgtcccct cgcctctccc aaaaacgcaa aaaagacaaa accccatacc
     2461 attacggtat ggggttttcc aagtggagga tgggggactc gaaccctcga cctctgcgat
     2521 gtgaacgcag cgctctaacc agctgagcta atcatccata tgcgattgac aaatgatatt
     2581 atataggatt tgcctgcatt tgtcaagcgc aaattttgaa atgttttgca cagaatcttg
     2641 acctggacgc ctattaaaat ttttccaaaa attttgcaaa aaaccgagac aaattcctaa
     2701 agttgtgcta tactgtgtca agaataacaa cccacacggt tttatcagga ggtttg
//
LOCUS       DBMDSQ010000264         2755 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_8158_length_2755_cov_1.859457, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000264 DBMDSQ010000000
VERSION     DBMDSQ010000264.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 2755)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2755)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2755
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8158_length_2755_cov_1.859457"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..827)
                     /gene="asnB"
                     /locus_tag="ACZPAX_08700"
     CDS             complement(<1..827)
                     /gene="asnB"
                     /locus_tag="ACZPAX_08700"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0070981 - L-asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HIR1114015.1"
                     /translation="MCGIAGFISPARADAAALVPMLARIAHRGPDGQGTFVEGPAALG
                     HCRLAIIDLQGGAQPLYSEDKNFVVVFNGEIYNYRELTAELTALGHTFATRTDTEVLL
                     HGWEQWGRELLPRLRGMFAFALWDRRAQVLFCARDMFGIKPLYYCRCADGTLLFASEI
                     KAFLDHPSFEKQLNTAQLPLYLSCQYSPGRDTFFAGVQKLLPGHFLEFSEGIVRTTRW
                     VQPAFLPGDTPPAPDELEAVLRQSAEAHKIADVEVAGFLSGGVDSAYLTALARPART"
     gene            950..2131
                     /locus_tag="ACZPAX_08705"
     CDS             950..2131
                     /locus_tag="ACZPAX_08705"
                     /inference="COORDINATES: protein motif:HMM:NF024949.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1114016.1"
                     /translation="MTLGQNIQTARRAKGLSQEALAERIGVSRQALGKWEKDTALPGL
                     DNLQALAAELGTGVDALLGAEQADPAAPAVTLDALRDLLAARDAEEKRRRRLWGCAGG
                     AAVVILAGLLAAVAWQYERQIAALNQNYAAIQSSYAAQQAELSAQISELQDAVRMGEA
                     TVLDWRWLPADKLHRDADKSWMPVQVQVTPRTVQDGMTARLAVRSGDDTQLYDMTAAP
                     DGSFAAQGIVFTVGSTYDLSVQWTADGTTTSEGLGTVDFNDEMTEPTIVWGAAGSSLD
                     FGYCVNRVNGKRYLTLTCYPVELQVDAPPWMQIAKVEIDLRLNGDAGEPTATAALDCE
                     GEYSYGSSARTESVWNGTFYDDAVINGWEYDGDAAPKYVVRVTDSNGGVWTEEMPLVE
                     K"
     gene            2336..>2755
                     /locus_tag="ACZPAX_08710"
     CDS             2336..>2755
                     /locus_tag="ACZPAX_08710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate--amine ligase"
                     /protein_id="HIR1114017.1"
                     /translation="MPQILPVLLGGDANVYGMARSFYETYGVRSLAVCRRALPALAHS
                     RIVQVAAQDPAFERDSVFTATLLGLAEQYPGRTRLLISCADGYTQLMARCAAELRGTY
                     RFACPPPAALALADKQQFAAACRAVGLRTPQSITVYPG"
BASE COUNT          509 a          864 c          933 g          449 t
ORIGIN      
        1 taggtccgcg cgggccgggc caatgccgtg aggtaggccg agtccacccc gccggagagg
       61 aaccccgcga cctcgacgtc ggcaattttg tgggcctcgg cactttggcg cagcacggct
      121 tccagctcat cgggggcggg cggcgtatcg cctggcagaa aggcgggctg tacccaccgc
      181 gtggtgcgca cgatcccctc ggaaaattcc aaaaaatgcc cgggcagcag cttttgcacc
      241 ccggcaaaaa acgtgtcccg ccccggggaa tactggcagg ataagtagag cggcagctgc
      301 gcggtgttga gttgcttttc aaacgaggga tgatccagaa acgccttgat ctcgctggca
      361 aacagcagcg tgccatcggc gcagcggcag tagtagagcg gctttatgcc gaacatatcc
      421 cgggcgcaga aaagcacctg cgcccggcgg tcccacagcg caaaggcgaa catcccccgc
      481 agccggggga gcagttcgcg gccccactgc tcccacccgt gcagcagcac ctcggtgtcg
      541 gtgcgggtgg caaaggtgtg gcccagggcg gtcagctcgg cagtcagttc gcggtagttg
      601 tagatttcgc cgttgaacac gacgacgaaa tttttatcct cgctgtacag cggctgtgcg
      661 ccgccctgca aatcaatgat ggccaggcgg cagtggccaa gcgcggcggg tccctccaca
      721 aacgttccct ggccgtccgg cccgcggtgg gcgatgcgcg ccagcatcgg gaccagcgct
      781 gcggcgtccg cccgggcggg ggagataaaa ccggcaattc cgcacatatt ccagcatcct
      841 ttcccggcag tgcaacctgc cgttaccgtt ttgtacactt gacttgctgg cattttacca
      901 cacgctgtgg taaaattatg tcaaatcaaa gcggaaagca ggtgagcctg tgacactggg
      961 gcagaatatc caaacagcgc gcagggccaa gggcctgagc caggaggcat tggccgagcg
     1021 catcggcgtc agccgccagg ccctgggcaa gtgggagaag gacaccgccc tgccggggct
     1081 ggacaatttg caggcgctgg cggccgaact gggcaccggt gtggatgccc tgctgggcgc
     1141 agagcaggcc gatcccgccg cgcctgccgt gacgctggat gccctgcggg acctgctggc
     1201 cgcccgcgac gccgaggaaa agcgccgccg cagactgtgg ggctgcgccg ggggcgcggc
     1261 ggtcgttatc ctggccgggc tgctggcggc ggtggcctgg cagtatgagc ggcagatcgc
     1321 cgcgctgaac cagaactatg cggccatcca gtccagctac gcggcccagc aggcagaact
     1381 ttcggcgcag atctccgaat tgcaggacgc cgtgcggatg ggcgaggcca ccgtgctgga
     1441 ctggcgctgg ctgcccgccg acaagctgca cagggacgcg gataagagct ggatgcccgt
     1501 gcaggtgcag gtcacgccgc gcacggtgca ggacggcatg accgcccggc tggctgtgcg
     1561 cagcggcgac gacacgcagc tctatgatat gaccgccgcg cccgacggca gctttgcggc
     1621 gcagggaatt gtttttacgg tgggcagcac ctacgatttg agtgtgcagt ggaccgccga
     1681 tggtacgacc accagtgagg gcttaggcac ggtggacttc aacgacgaaa tgaccgagcc
     1741 gacgattgtc tggggtgcgg cgggcagcag cctggatttc gggtactgcg tcaaccgggt
     1801 caacggcaag cggtatctga ccttgacctg ctacccggtg gaacttcagg ttgacgcccc
     1861 gccgtggatg cagattgcga aggtggaaat tgacctgcgc ctgaacggcg acgccggcga
     1921 acctaccgcc accgccgcgc tggattgcga gggcgagtac agctacggca gctccgcccg
     1981 caccgagagc gtctggaatg gcacgtttta cgatgatgcc gtcatcaacg gctgggagta
     2041 cgacggcgat gccgcgccca aatacgttgt acgggtgacg gacagtaatg gcggggtatg
     2101 gacggaggag atgccgctgg tagagaaata atgtgccttc ccccttgggg gaaggtgccc
     2161 gaggccccga ccgagggcgg atgaggggca gccttgccaa cagaacccat ttgccgggcg
     2221 gctgtggtat acctgcccct catccggccc tgcggggcca ccttctccct aggggagaag
     2281 gcaaaaagaa taaacgcccc cttgccgcat acactagcgg taagggggaa tttctatgcc
     2341 gcagatattg ccggtgctgc tgggcgggga tgccaatgtc tacggtatgg cccgcagctt
     2401 ttatgagaca tacggcgtgc gcagcctggc cgtctgccgc cgtgcgctgc ctgcgctggc
     2461 ccacagccgt attgtgcagg tggccgccca ggacccagcg tttgagcggg acagcgtttt
     2521 caccgccacg ctgctgggcc tggccgagca ataccccggc cgaacgcggc tgctcatctc
     2581 ctgcgccgac ggctacaccc agcttatggc ccgctgtgcc gccgaactgc gggggacgta
     2641 ccgctttgcc tgcccgccac ccgccgcgct ggccctggcc gataaacagc agtttgccgc
     2701 cgcctgccgc gccgtgggcc tgcgcacgcc gcagagcata acagtatacc cgggg
//
LOCUS       DBMDSQ010000265         2744 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_8217_length_2744_cov_1.615588, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000265 DBMDSQ010000000
VERSION     DBMDSQ010000265.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 2744)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2744)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2744
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8217_length_2744_cov_1.615588"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1357)
                     /locus_tag="ACZPAX_08715"
     CDS             complement(<1..1357)
                     /locus_tag="ACZPAX_08715"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR1114018.1"
                     /translation="MKFATKLSLGCIALLSCALGAAGLLLTGQSFSGSLASTRTALQA
                     QQEKEKYALERFIFQATDSAQFENYILASAAQQYAEQTADAGSSMALWLDGAYALYSG
                     LPAALPHTALKQALTDGENAWQLTRAAGRWYLLLTQPLDLPGVRADMLCAYDVSAVFA
                     TRDAQLRAWLAASAALLVLGGGASVLFSRRVTRPLTALQTASEKIADGEYTQRTQVKT
                     DDEIGALSRSFDAMAAAVEGKINELSKTTQSQQDFIAAFTHEVKTPMTAMLGYADLMR
                     ARPDDTETQREAAGYIYHETQRLENLSRSLLALMGLDQAGALELVPCQDAGLLLQTVR
                     SLPQGSTPVPRVISAGCVVRVDRSLWVDMLRNLILNAQRACQGIEGAAITLRCERRAG
                     QAVFTVTDTGCGIPAADLPRVTEAFYMVDKSRSREYGGAGLGLSLAWEIANLHGGNIW
                     VE"
     gene            complement(1368..>2744)
                     /locus_tag="ACZPAX_08720"
     CDS             complement(1368..>2744)
                     /locus_tag="ACZPAX_08720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1114019.1"
                     /translation="KQRGITTMKHTARLTVCLLALALTACTAAPQDMPAGTTTETAAA
                     PAQTAEPTTETPAVQDTLTPLYTTWGAAVGRDAVYSAMNIDDAGQYFATTIDFSTGEQ
                     RILCNKFGCNHADESCPAYMTAIRNGCVPEAMLLPMGDRIYWLVDGRYNESDGAYLDV
                     SNTDGSDRRRVAEGDDLPDLTNAFVWYTDGTALYTFVRSYSEFTVLRLDEGGAACFFT
                     HSIPDGEDYVAVGCWQDKIVLQHSACYQQQPLNPAGEAATDEELRAWLAADEQARNEQ
                     TKNLCLLDTAGNMTDTDMTWSGDAGNVQLVRNGAAYLLNESGLVTKFDLTAGTTQTRQ
                     LDLQGTLVLYGVVEGARPDGWIPVTVRDDSEGLLNPETGVYTPLPTTWLKDQAVERSP
                     FIVAASDTMLLVKYGEIYYTTNDIGTDGAPYSFTTCRGEYGIISAADYLAGSQDWVQV
                     TLQGRDLV"
BASE COUNT          482 a          895 c          814 g          553 t
ORIGIN      
        1 tctccaccca aatattgccg ccatggagat tggcgatctc ccacgccaaa gaaagcccca
       61 aaccagcgcc gccgtactcc cggctgcggg acttgtccac catataaaat gcctctgtca
      121 cgcggggcag gtcggcggcg ggtatgccgc agcccgtgtc cgtgacggtg aaaaccgcct
      181 gcccggcgcg gcgctcacac cgcagggtga tggccgctcc ctcaatgccc tgacaggcgc
      241 gctgggcgtt gaggatcagg ttgcggagca tatctaccca caggctgcgg tctacccgca
      301 ccacgcaccc cgcgctgatg acacgcggca caggcgtgct gccctgcggc aggctgcgca
      361 cggtctgcag caaaagcccg gcatcttggc agggcaccag ctccagcgcc ccggcctgat
      421 ccagccccat caaggccagc agactgcggc tcaggttctc cagccgctgc gtctcgtggt
      481 agatatagcc cgctgcttcc cgctgagtct cggtgtcatc gggccgggcg cgcatcaggt
      541 cggcatagcc aagcatcgcg gtcatcgggg tcttgacctc atgtgtaaac gccgcaataa
      601 agtcctgctg gctttgcgtt gttttcgata attcgttgat cttgccttcc accgctgcgg
      661 ccattgcatc aaagctgcgg ctcagtgcgc cgatttcgtc atcggtttta acctgagtac
      721 gctgggtgta ctcaccatcg gcaattttct cgctggccgt ctgcagcgca gtcagcgggc
      781 gggtcacacg gcggctgaac agcacagatg ccccaccgcc caataccagc agcgctgccg
      841 atgccgccag ccacgcccgc agctgggcgt cacgggtggc aaacaccgcg ctcacatcgt
      901 aggcgcagag catatcggcc cgcacgccgg gcagatccag cggctgggtc agcagcagat
      961 accaccgccc ggccgcccgg gtcagctgcc aggcgttttc gccgtcagtc agggcctgct
     1021 tcagtgcggt gtgcggcagg gccgcaggca ggccggaata gagcgcatac gcaccgtcca
     1081 gccagagtgc catgctgctg cccgcatcgg cggtctgctc ggcatactgc tgggccgcgg
     1141 acgccaaaat gtaattttca aactgcgcac tgtccgtggc ctgaaagatg aaccgctcca
     1201 gcgcgtattt ttctttttcc tgctgggcct gcagggccgt gcgggtgctg gccagactgc
     1261 cggaaaagct ctgcccggtc agcagcaggc ctgctgcgcc cagcgcgcag gacagcagcg
     1321 caatgcagcc cagagatagc ttggttgcaa atttcatcac tgtttcctca taccagatcg
     1381 cggccttgca gtgtcacctg cacccaatcc tgactgcccg ccagatagtc tgctgccgaa
     1441 atgatcccgt actctccgcg gcaggtggta aagctgtaag gtgcgccgtc cgtgccgatg
     1501 tcgtttgtcg tgtagtagat ctcgccgtat ttcacaagca gcatcgtgtc gctggctgcc
     1561 acaataaacg ggctgcgctc cactgcctga tccttgagcc atgtggtcgg caacggggtg
     1621 tagacgccgg tctcggggtt cagcagcccc tcgctgtcat cgcgcaccgt cacagggatc
     1681 cagccgtccg gccttgcgcc ttccacgaca ccgtataata ccagtgtccc ctgcaggtca
     1741 agctgtcggg tctgggtcgt gccagcagtc aggtcgaatt ttgtcaccag cccggattcg
     1801 ttcagcagat aggccgcacc gttgcggaca agctgaacat tgcctgcatc gccgctccat
     1861 gtcatgtcgg tgtcggtcat gttgcccgcc gtatccagca gacagaggtt tttcgtctgc
     1921 tcgttccggg cctgctcatc ggcagccagc catgcgcgca gttcctcatc cgtggcggcc
     1981 tctccggccg ggttcaaggg ctgctgctga taacatgcgc tgtgctgcaa gacaattttg
     2041 tcctgccagc agcctacggc gacataatcc tcgccgtccg gtatgctgtg ggtaaagaag
     2101 cacgccgcac cgccctcatc cagacgcagg acggtaaatt cactgtagct gcgcacaaaa
     2161 gtatacagtg ctgtgccatc cgtgtaccag acaaaggcat tggtcaaatc aggtaggtcg
     2221 tctccctcgg ccacgcggcg gcggtcactg ccgtccgtat tgctcacatc cagatatgcg
     2281 ccgtcactct cattgtaacg gccatccaca agccagtaaa tccggtcgcc catgggcagc
     2341 agcatggctt ccggcacgca gccgttccgg atggccgtca tgtaggccgg gcagctttca
     2401 tccgcatggt tgcagccgaa tttattgcag aggatgcgct gctcacccgt ggaaaaatca
     2461 atcgttgtgg cgaagtattg gccggcatcg tcaatgttca tcgcgctgta gacggcatcc
     2521 cgccccaccg ccgcgcccca ggtcgtgtaa aggggcgtaa gcgtgtcctg cacggcgggt
     2581 gtttctgtgg tgggctctgc agtctgcgcg ggcgcggcgg ctgtctcggt ggtcgtgcct
     2641 gcaggcatgt cctgcggcgc agccgtgcag gccgtcagcg ccagcgccag cagacagacg
     2701 gtcagccttg cggtgtgttt catcgtggtt atcccccttt gttt
//
LOCUS       DBMDSQ010000266         2733 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_8247_length_2733_cov_2.407675, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000266 DBMDSQ010000000
VERSION     DBMDSQ010000266.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 2733)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2733)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2733
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8247_length_2733_cov_2.407675"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1277
                     /gene="mgtE"
                     /locus_tag="ACZPAX_08725"
     CDS             <1..1277
                     /gene="mgtE"
                     /locus_tag="ACZPAX_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HIR1114020.1"
                     /translation="TLPAPDLAAVFEDLPPEKLPVLFRLCPKDLAAEIFAELTPETQQ
                     NLIDGLTDKELKAVVDELFVDDATDLVEEMPANVVKRILGQADPATRRMINELLKYPE
                     DSAGGVMTTELMELRPDWTVAQAMDAIRKNGFDKETINNCYVTDRDRTLIGVVSLRAL
                     VLSKDPQNEIIRDMMDDNVVSVATTTDQEDVSQMFEKYGYLAIPVVDNEKRLVGIVTI
                     DDAISIMQDEASEDIAKMNAIGPSDKPYFKQSMWDLYKSRAPWLLFLMISSTFSSMVI
                     RGYEDALAAVTVLTAYIPMLTDAGGNAGSQSTSTIIRGLAVGDIEPHDLPKILWREFR
                     IAILCGGTLALCNFAKLIFLDGIKAPVAAVVCLTLVCTIILSQLIGGLLPVVAEKFKM
                     DPAVMASPLITTIVDTTTLLVYFNIARVLLKI"
     gene            1521..>2733
                     /locus_tag="ACZPAX_08730"
     CDS             1521..>2733
                     /locus_tag="ACZPAX_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009326055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HIR1114021.1"
                     /translation="MNMQDFYTGHAFDAYEFFGAHTYFGGTHFAVWAPSAQHIAVETE
                     IGTFDMHRTQSAVWECDAPGVNAGMVYQYWVRGANGQSVAHCDPYGTAMTLRPDGRSI
                     VSDRPQGFDDDQWMQARTKCFDKPLNIYEVHAGSWRQKEDGSWYTYAELAELLPAYCR
                     ENGFTHIELMPLAEHPFDGSWGYQITGFFAPTSRYGTPDELAEFVNACHKQGIGVILD
                     FVPVHFAVDAYGLKEFDGTPLYEYPAAAVGQSEWGSCNFMHSRGEIQCFIQSCADYWL
                     RVFHADGLRMDAVSRLIYWQGDPNRGVNGSTLDFLKGMNCGLQQRHPTAILIAEDSTS
                     FPKVTAPVEYGGLGFDYKWDLGWMNDTLDYFKKTPDERRENLGKLTFSMMYAWNEHYI
                     LPFSHDENVHGK"
BASE COUNT          553 a          855 c          785 g          540 t
ORIGIN      
        1 ccaccctgcc cgcgcccgat ctggccgccg tgtttgagga tctcccgccc gaaaagctcc
       61 ccgtgctgtt ccgcctgtgt cccaaggacc tggcggccga gattttcgcc gaacttaccc
      121 ccgagaccca gcagaacctc atcgacggcc tgaccgataa ggaattgaag gccgttgtgg
      181 acgaactgtt cgtcgatgat gcgacggatt tggtcgagga aatgcccgcc aatgtagtca
      241 agcgtatcct tggccaggcg gaccccgcga cccgccgcat gatcaacgaa ctgctcaaat
      301 accccgagga ctccgccggt ggcgtcatga ccactgagct gatggagctg cgccccgact
      361 ggaccgtggc ccaggccatg gacgccatcc gcaagaacgg ctttgacaag gagaccatca
      421 acaactgcta cgtcaccgac cgtgaccgca ccctcatcgg tgtcgtctcc ctgcgcgcac
      481 tggtcctctc gaaggatccg cagaacgaga tcatccgcga catgatggat gacaacgtcg
      541 tcagcgttgc caccaccacc gaccaggagg acgtctccca gatgttcgag aagtacggct
      601 acctggctat tcccgttgtg gacaacgaga agcgtctggt cggtatcgtc accatcgacg
      661 acgctatctc gatcatgcag gacgaggcca gcgaagatat cgccaagatg aacgctatcg
      721 gcccgtcgga taagccctac tttaaacagt ctatgtggga tctgtacaaa agccgtgcgc
      781 cgtggctgct gttcctgatg atttcctcca ccttctccag catggtcatc cgcgggtatg
      841 aggatgccct cgccgccgtg acggtgctga ccgcctacat cccgatgctg accgacgcag
      901 gcggcaacgc gggcagccag agtacttcca ccatcatccg cggcctggcc gtcggtgaca
      961 ttgagcccca cgacctgccg aagattctgt ggcgcgagtt ccgtattgcc atcctctgcg
     1021 gcggcacgct ggcgctgtgc aactttgcca aactgatttt cctggacggc atcaaggctc
     1081 ctgtggcagc ggtcgtctgc ctgacgctgg tctgcacgat catcctgtcc cagctgatcg
     1141 gcggcctgct gcctgttgtg gccgaaaagt tcaagatgga cccggccgtc atggcgtcgc
     1201 cgcttatcac caccatcgtc gataccacga cgctgctggt ctacttcaac attgcacggg
     1261 ttttgctgaa aatttaaatt ttggacaccg tgttcaaaat tgtaggttgt accacacctt
     1321 tgggtgtggt acaatttttt tagagaaaag gccttcccct gagggggagg gtgccccgaa
     1381 ggggcggatg aggggcagac ttcccgcagc aatccattac cgggctatgg cggaaccgcc
     1441 gccccttatc agtcagcggt cggggccgct gacagcttcc cccagggggg aagccatcct
     1501 tacaatagga gactttcatc atgaatatgc aggattttta caccgggcac gcctttgatg
     1561 cgtatgaatt tttcggggcg catacctatt tcggcggcac acattttgca gtgtgggcac
     1621 cgtccgcgca gcatatcgct gtcgagaccg agatcggcac ctttgacatg caccgcaccc
     1681 agtccgccgt atgggaatgc gacgcccccg gtgtgaacgc gggtatggtc taccagtatt
     1741 gggtgcgcgg cgcaaacggc cagagcgtgg cccactgcga cccctacggc acggctatga
     1801 cactgcgccc cgacggccgc agcatcgtgt ccgaccgccc gcagggcttt gacgacgacc
     1861 aatggatgca ggcacgcaca aaatgcttcg ataaaccgct gaatatctac gaggtccatg
     1921 ccggcagctg gcggcagaag gaggacggca gctggtatac ctacgccgaa ctggccgagc
     1981 tgctgcccgc ctactgcagg gagaacggct ttacccacat tgaactcatg cccctggccg
     2041 agcacccctt cgacggcagc tggggctacc agatcaccgg cttttttgcc cccaccagcc
     2101 gctacggcac gccggacgag ctggccgaat ttgtcaacgc ctgccacaag cagggcatcg
     2161 gcgttattct ggattttgta ccggttcact ttgccgtgga cgcctacgga ctgaaagaat
     2221 ttgacggtac accgctgtat gagtacccgg cagccgctgt gggccagagc gagtggggga
     2281 gctgtaactt tatgcactcc cgcggtgaga tccagtgctt catccagtcc tgcgccgatt
     2341 actggctgcg ggtgttccac gccgacggcc tgcgcatgga cgcggtgtcc cggctcatct
     2401 actggcaggg cgacccgaac cgcggcgtca atggctcgac actggacttt ttgaagggca
     2461 tgaactgcgg tttgcagcag cgccacccca cggccatcct gattgccgag gactccacca
     2521 gcttcccgaa agtgaccgcg ccggtcgagt acggcggcct gggctttgac tacaagtggg
     2581 atctgggctg gatgaacgat accctagact actttaaaaa gaccccggac gagcggcggg
     2641 agaacttggg caagctgacc ttctcgatga tgtacgcctg gaacgagcac tacatcctgc
     2701 ccttcagcca cgacgagaac gtccacggca agg
//
LOCUS       DBMDSQ010000267         2718 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_8309_length_2718_cov_1.344287, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000267 DBMDSQ010000000
VERSION     DBMDSQ010000267.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 2718)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2718)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2718
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8309_length_2718_cov_1.344287"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..947
                     /locus_tag="ACZPAX_08735"
     CDS             <1..947
                     /locus_tag="ACZPAX_08735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1114022.1"
                     /translation="SIIRESYAKCRLSGAKQVGGIAGNGATIENCRAMVIIDNAAEQL
                     GAIAGYVEDPLDGSVTGNTFVDEGVAGIDSVSYKNIAEPLSYADFAAQENLPSDFASI
                     CVRFVTEDDTLVQQYYIPYGSDFPTDQLPPVPNHTDQYGSWEDVDLTNMTFDATIHAE
                     YSDMNTVRQSQEKRDGRPIVLVEGSFDTTDELMLHEVDDAPAALGVLVEAWGLELPAD
                     TGHTLRYMPPETTDNTELWVKTDAGWQQAETSVDGSYLTCTAPAGTTEFAAVKIPASK
                     VPLAAAAYGAAAALLLVILFIVRKRKKRKAKKAAEKAE"
     gene            complement(1138..1839)
                     /locus_tag="ACZPAX_08740"
     CDS             complement(1138..1839)
                     /locus_tag="ACZPAX_08740"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013624227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HIR1114023.1"
                     /translation="MKRPLIGIVPLVDAQRESYWMLPGYMRGVEQAGGLPIMLPLTDD
                     DAALRQLADTCDGFLLTGGQDVSPALYGAAPVPACGETCPARDAMETKLLALALEQDK
                     PVLGICRGIQFLNVYLGGTLYQDLPTEHPSAAEHHQKPPYDVPVHSVTLVPARPLYQL
                     LGKCELAVNSLHHQAIRKLAPTLQVMALSEDGIPEAICLPGKKFVWAVQWHPEFSFPI
                     NADSRKIFAAFVGAC"
     gene            complement(1941..2387)
                     /locus_tag="ACZPAX_08745"
     CDS             complement(1941..2387)
                     /locus_tag="ACZPAX_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR1114024.1"
                     /translation="MNLLFFLTPKQDVLYIYEDFTLRQTLEKWSNQRYATIPVLKRNG
                     EYVGSITEGDILWGMKNLHGLDLEASEDVPISSFPRRRDYKAVTVTTDMPTLLKAAID
                     QNFVPVVDDRNVFIGMVRRTVLLRELYEKYTTLPTDKRFKANHPPM"
BASE COUNT          594 a          834 c          776 g          514 t
ORIGIN      
        1 agtccatcat ccgcgaaagc tacgccaagt gccgcctgtc cggcgcaaag caggtaggcg
       61 gcatcgccgg aaacggcgcg acgattgaga actgccgcgc tatggtcatc atcgacaacg
      121 ccgccgagca gctcggcgcg attgccgggt atgtggagga cccgctggac ggcagcgtca
      181 ccggcaacac cttcgtggac gagggcgtgg ccggcatcga cagcgtcagc tacaaaaaca
      241 ttgccgagcc gctgagctat gcggattttg ccgcacagga gaacctgccc agcgactttg
      301 ccagcatctg cgtccgcttc gtgaccgagg atgacacgct ggtgcagcag tattacatcc
      361 cctacggcag cgacttcccg acagaccagc tcccgccggt gcccaaccat acggaccagt
      421 acggcagctg ggaggacgtt gaccttacaa acatgacctt cgacgcgacc atccacgccg
      481 agtacagtga catgaacacc gtgcgccaga gccaagagaa gcgcgatggc cgccccatcg
      541 tattggtgga gggcagcttt gacaccaccg acgagctgat gctccacgag gtggacgacg
      601 cacccgcagc cctcggcgtt ctggtcgagg cctggggcct ggaactgccc gccgacacgg
      661 gccacacgct acgctacatg ccgccggaga cgaccgacaa caccgagctg tgggtcaaga
      721 ccgacgcagg ctggcagcag gccgaaacga gcgtggacgg cagctacctg acctgcactg
      781 cccccgccgg gacgacggag tttgccgccg tcaaaattcc cgccagcaag gtcccgctgg
      841 ctgccgctgc ctacggtgcc gccgccgcgc tgctgctggt gattttgttc atcgtgcgca
      901 agcgcaaaaa gcgaaaagca aaaaaagctg ctgaaaaggc agaataaaag aaagtccctt
      961 ctgtggtgtg acaccacaga agggactttt ttggcttccc ccttggggga agctgtcacc
     1021 gaaggtgact gatgaggggc agccttccca cggcaaccta tttatgggca ataatggcaa
     1081 acttgcccct catccggcgc tccgcgccac cttccccctt gggggaaggc ttttccttta
     1141 acacgcaccc acaaacgccg caaaaatctt gcggctgtcc gcattgatgg ggaacgaaaa
     1201 ctccgggtgc cactgcacag cccagacaaa cttcttgccc ggcagacaga tggcctcggg
     1261 gatgccatcc tccgacaggg ccattacttg cagcgtcggg gccagtttgc ggatggcctg
     1321 gtggtgcagg ctgttcacgg ccagctcaca cttgcccaac agctggtaga gcggccgcgc
     1381 cggcaccagc gtgacactgt gtaccggcac atcgtagggc ggcttctggt ggtgctccgc
     1441 cgccgagggg tgctcggtgg gcaggtcctg gtacagcgtg ccgcccaggt agacgttcaa
     1501 aaactggatg ccccggcaaa tgcccagcac cggcttgtcc tgttccaggg ccagcgccag
     1561 cagctttgtc tccatggcgt cacgcgcggg gcaggtctcg ccgcaggcgg gcacaggggc
     1621 cgcgccgtac agggcagggg agacatcctg gccgccggtc agcaaaaatc cgtcgcaggt
     1681 gtcggccagc tggcgcagag cggcgtcatc gtcggtcagc ggcagcataa tgggcaggcc
     1741 gcccgcctgc tccacacccc gcatataacc gggcagcatc cagtaactct cccgctgggc
     1801 atccaccagc ggcacaattc caatcaaagg ccgtttcatt cttcccacac ctcactgtaa
     1861 aaaataaaac agagaagccc ttgcgtgact tctctgtccg ttgtgctgat tataccgcgc
     1921 cgcgcggcaa aatgcaagcc ctacatcggc gggtggttcg ccttaaaccg cttgtccgtc
     1981 ggcagggtcg tgtatttttc gtacagctcc cgcagcaaaa cggtacggcg caccatgccg
     2041 ataaacacgt tgcggtcatc caccaccggc acaaagttct ggtcgatggc cgcctttaac
     2101 agcgtcggca tatcggtcgt gaccgtcaca gccttgtagt cacggcggcg gggaaaactg
     2161 gaaatgggta cgtcctcact ggcttccaga tccaggccgt gcaggttttt catgccccag
     2221 aggatgtcac cctcggtgat gctgcccaca tattcgccgt ttcgtttcag caccgggatg
     2281 gtagcgtagc gctggttcga ccatttttcc aacgtctggc gcagggtgaa atcctcgtaa
     2341 atgtacaaga catcctgctt gggtgtcaaa aagaacaaaa ggttcatatc ttaactactc
     2401 tcctttggta gtgttgtcat acttctgtaa ccagtatata cgtttgcact gtaaactttg
     2461 tgaatggaat gttaaatccg cgcaacaaaa taaatttctc cgaaatgccc tgttcaagcg
     2521 ggaaaaaatg gtgtataata gctaatgatt catatagtgc agctgcggct ttagtcgtgg
     2581 ctaaccccga aaaggaggat cgcagatgat caccaagctc aatccctatg gacatatttc
     2641 gctgaccaat gactacttct ccggcctggt cgagcaggcc gccaagcagt gctacggcat
     2701 tgccgccatg ggtcaggc
//
LOCUS       DBMDSQ010000268         2709 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_8344_length_2709_cov_1.836656, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000268 DBMDSQ010000000
VERSION     DBMDSQ010000268.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 2709)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2709)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2709
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8344_length_2709_cov_1.836656"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..512
                     /locus_tag="ACZPAX_08750"
     CDS             <1..512
                     /locus_tag="ACZPAX_08750"
                     /EC_number="3.2.2.-"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015565129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase family protein"
                     /protein_id="HIR1114025.1"
                     /translation="IIGQQISMKAQATIWQRMRDALGTVDAEHLLAAGVPQLQQLGMT
                     FRKAEYITDFSQKIHDGTFDLQAVWQMPDDEAIRALSSLKGIGVWTAEMILLFCMQRP
                     DVFSYDDLAIQRGLRMLYHHRNIDRKLFEKYRRRFSPYGSVASLYLWAISGGAIPGMR
                     DYAPKKPAK"
     gene            complement(659..1723)
                     /locus_tag="ACZPAX_08755"
     CDS             complement(659..1723)
                     /locus_tag="ACZPAX_08755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1114026.1"
                     /translation="MPDYQKDTPRQAALRREIETHAIWQYPPEERLQHNREWQSLYLL
                     VFEFKNFLQKIEHRLQNTDASALAQDEEAMLDARYTRRSQLRGKLDTLTFTQAMRTAV
                     AKFDTTSGKEFLAYFDAIYSNALHENVNKQATRDQGDIGLSSREGKLWKRVLDLCDKQ
                     GLDARRLPAPFYKQLADSLGLDEDALRRLIRNATSARRTFSLNEDGGDNGPAFDMADP
                     GQEDLQLRLERTTEAMRVIALFAGKDIKDYPRLFFTTDVLGPLCAAKPTFPPECYCAL
                     LEKQEDLLWRHIFVRGYIDFLFDPAHPARLRILPDTPLAHPLRDSSVAAYQHVTPAAV
                     SSQRKQYTALLHKMMAQMQG"
     gene            complement(1727..2344)
                     /locus_tag="ACZPAX_08760"
     CDS             complement(1727..2344)
                     /locus_tag="ACZPAX_08760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TM1812 family CRISPR-associated protein"
                     /protein_id="HIR1114027.1"
                     /translation="MKRIYITAIPLDSNFAIAPYAAQAANYQQTYTAPSCYPITPIIA
                     DTARPGDEITVLAVRQKNSPRSDNLDAFRRELDTLGTPHTLIDVTTPENQQRDALLTL
                     FETLTDAMQDDACYYADATFGTKTYPLVLASALRYAEKILDDTEVCGIYYRELTRENG
                     QVKTARQYDISTLFTLDGIVDMAAGEDIADKRAFIKLMLHPDREV"
     gene            complement(2461..>2709)
                     /locus_tag="ACZPAX_08765"
     CDS             complement(2461..>2709)
                     /locus_tag="ACZPAX_08765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1114028.1"
                     /translation="VCDTCGNTTTTVVGPKGVKGYVGSGAIAAVAPKASAPETRYATA
                     AEAQAAADKREAAYAAAVAAFQKQIDAQNAAYLAALGK"
BASE COUNT          471 a          766 c          901 g          571 t
ORIGIN      
        1 acatcatcgg gcagcagatt tcgatgaaag cgcaggccac catctggcag cggatgcggg
       61 acgccctggg caccgtggac gccgagcacc tgctggcggc gggggtgccg cagctgcaac
      121 agctgggcat gaccttccgc aaggcggaat acatcacgga tttttcacag aaaatccacg
      181 acggcacgtt tgatttgcag gccgtttggc agatgcccga cgacgaggcc atccgggcgt
      241 tgagcagctt aaagggcatc ggcgtctgga cggcggagat gattttgctg ttttgtatgc
      301 agcggcccga tgtgttcagc tacgacgatt tagcgataca gcgcggtttg cggatgctgt
      361 accatcaccg aaacattgac cgcaagcttt ttgaaaagta ccgtcgccgg ttcagcccct
      421 acggcagcgt cgccagcctc tatctctggg ccatctccgg cggtgccatc cccgggatgc
      481 gggactacgc gccaaaaaag ccagccaaat aaaaaagaag cgggagcagc tttgtgggaa
      541 agctgctctc gcgtttgggt tatgatgggt aacggcaagg ccgcgcagac ggagcaaagt
      601 cacacccctg tatttcgcta aattcgtctt tagcgaaatt agatgttgct ctcccccttc
      661 acccctgcat ctgcgccatc atcttgtgca gcagcgcggt gtattgctta cgctggctgc
      721 tgacggcggc aggggtcacg tgctggtagg cggctacgct gctgtcccgc agggggtgcg
      781 ccaagggcgt gtccggcagg atgcgcagcc gcgcggggtg ggcggggtca aacaggaaat
      841 caatgtaccc gcgcacaaaa atgtggcgcc aaagcaagtc ctcctgcttt tccagcaggg
      901 cgcagtagca ctctggcggg aaagtcggct tcgcagcgca gagtggtccc agcacgtcgg
      961 tggtgaaaaa cagccgcggg taatccttaa tatccttgcc cgcgaacagc gcaatcaccc
     1021 gcatggcctc ggtggtgcgc tccaggcgca gctgtaaatc ttcttgcccg gggtcggcca
     1081 tgtcgaaggc cgggccgttg tcgccgccgt cctcgttcag cgagaaagtg cgccgggcgc
     1141 tggtggcgtt gcggatgagc cgtcgcagcg cgtcctcgtc caggcccagg ctgtcggcca
     1201 gttgcttata aaagggggct ggcaggcggc gggcgtccag gccctgcttg tcgcacagat
     1261 ccagcacccg cttccacagt ttgccctccc gagaggaaag cccaatgtcg ccctggtcgc
     1321 gggtggcctg cttgttgacg ttctcgtgca gggcgttact gtaaatcgcg tcaaaatacg
     1381 ccagaaactc cttgccagag gtggtgtcaa acttggccac ggcggtgcgc atggcctggg
     1441 tgaaggtcag ggtgtccagc ttgcctcgca actggctgcg gcgggtgtag cgggcgtcca
     1501 gcatggcttc ctcgtcctgg gccagggcgc tggcgtcggt gttttgcagg cggtgctcga
     1561 ttttctgtaa gaagttttta aactcaaaca ccaacagata caggctctgc cactcgcggt
     1621 tgtgctgcag gcgctcctct ggcgggtact gccagatggc gtgcgtctcg atttcgcggc
     1681 gcagggcggc ctgcctgggg gtgtcttttt ggtagtccgg catggctcac acctcccggt
     1741 cggggtgcag catcaacttg ataaaggcgc gtttatcggc aatgtcctcc cccgctgcca
     1801 tgtccacgat gccgtccagc gtgaacagcg tgctgatatc gtactggcgg gcggttttga
     1861 cctggccgtt ctcgcgggtc agctcgcggt aatagatgcc gcaaacctcc gtatcatcga
     1921 ggattttctc agcataccgc agcgccgagg ccagcaccag cgggtaagtt ttggtgccga
     1981 aggtcgcgtc ggcgtagtag caggcgtcgt cctgcatggc gtcggtcagc gtctcgaaca
     2041 gggtcagcag ggcgtcgcgc tgctggtttt ccggcgtagt tacgtcaata agtgtgtggg
     2101 gcgtgcccag ggtgtccagc tcgcggcgga acgcatcgag gttgtcgctg cggggactgt
     2161 ttttctggcg cacggccagt actgtgatct cgtcgccggg acgggcggtg tcggcgatga
     2221 tgggcgtgat gggatagcag gacggcgcag tgtaggtttg ttggtagttg gccgcctgcg
     2281 ccgcgtaggg tgcgatggca aaattgctgt ccagcgggat agcggtgatg tagatacgtt
     2341 tcataggatg cgtcctttcc tgccgtgcgc ggcaacactt ttttgtatta tagcaaattt
     2401 aggcggggga ttcaatgaga aaaggggcgc accctcccgg tgtgcccctt ccctattggg
     2461 ttacttgcct aacgcggcca ggtaggcggc attctgcgcg tcgatctgtt tctggaacgc
     2521 agccacggcg gcggcgtaag cggcctcgcg cttgtcagcg gctgcctgtg cctcggcggc
     2581 ggtggcgtag cgggtctcgg gggcggaagc cttgggcgca acagcggcga tcgcaccgct
     2641 gccgacatag cccttcacac ccttggggcc gacgactgtg gtggtcgtgt tgccgcaggt
     2701 gtcgcaaac
//
LOCUS       DBMDSQ010000269         2704 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_8373_length_2704_cov_2.076450, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000269 DBMDSQ010000000
VERSION     DBMDSQ010000269.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 2704)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2704)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2704
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8373_length_2704_cov_2.076450"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..354)
                     /locus_tag="ACZPAX_08770"
     CDS             complement(<1..354)
                     /locus_tag="ACZPAX_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015537430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HIR1114029.1"
                     /translation="MFNALFTKTEEYAKLAQALGGPGACALFGIPGAGRALVYAALSQ
                     ALDRPLCIVTPGEAEATRFAGDLNALGVAAAVFPARDYVLRPIEGTAREYEYRRLAVL
                     GDLVGGRLQAVCVPDE"
     gene            complement(361..903)
                     /locus_tag="ACZPAX_08775"
     CDS             complement(361..903)
                     /locus_tag="ACZPAX_08775"
                     /inference="COORDINATES: protein motif:HMM:NF019844.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HIR1114030.1"
                     /translation="MSELRKKAMTRLAICAVALVASIIFSVLLLTGTFDGKTDSGDAA
                     PATSDTTSAAAGVNDPTADSFTAEATGDTAEPPAEEQPAEEQPAEPEPTPEPTPVPTF
                     DGLSVQPTTYYNEGEQPSYTTPGDSGMNLRAGPGTDYDKVTSVPAGTGVTALGTNEDG
                     SWVVVNYDGKYGWLKTEFLS"
     gene            complement(918..1505)
                     /gene="pth"
                     /locus_tag="ACZPAX_08780"
     CDS             complement(918..1505)
                     /gene="pth"
                     /locus_tag="ACZPAX_08780"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - peptidyl-tRNA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HIR1114031.1"
                     /translation="MLGMTSGADWLIAGLGNPEPKYDGTRHNAGFEALDYLAAQWGCS
                     ITKAKWQGLYGTAQVGQHKVVLLKPLTYMNLSGQSIAPAANFYKIPADHVIVLCDDIT
                     QEPGHLRIRPHGSAGGHNGLKSIIASLDTENFNRIRIGIGAKPNPQYDLAAWVLGKLP
                     PADRKAMTDRYPDIEDACKLLMDGNLQYAQNKFNH"
     gene            complement(1571..2305)
                     /locus_tag="ACZPAX_08785"
     CDS             complement(1571..2305)
                     /locus_tag="ACZPAX_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine diphosphorylase"
                     /protein_id="HIR1114032.1"
                     /translation="MSTAKEKFELTQQRYVATFERHLDNGVNFISRDVYIEPEVVIAP
                     GATILPGCILRGKTVIGADCVIGPNTLLENTVVDAGSTINASQCYDSHIGPNNKIGPF
                     THVRTGTKTAEGCHLGAYVETKNADFAEGNTVSHLTYIGDATVGKYCNFGCGTVTCNY
                     DGEGKFHTTIGDYAFIGCNTNLVAPVTVGDHAFTAAGSTIGHDVPAGALSIERAKQAE
                     IEGWGEKKLEKYIAKKQKLEAEKKAK"
BASE COUNT          490 a          768 c          857 g          589 t
ORIGIN      
        1 ctcgtccggc acacagacgg cctgcagacg gccgccgacc agatcgccca gcacggccag
       61 gcggcggtac tcgtactcgc gggctgtacc ctcaatgggg cgcagcacat agtcgcgggc
      121 ggggaacacc gccgccgcca cgcccagggc gttcaggtcg cccgcaaagc gggtggcctc
      181 ggcctcgccg ggggtcacga tgcagagcgg cctgtccagt gcctgggaaa gcgcggcgta
      241 aaccagcgcc cgccctgccc cgggaatgcc aaataaggcg caagcccccg gtcccccgag
      301 cgcctgcgcc agttttgcgt attcttcggt tttggtaaaa agtgcgttga acatagctta
      361 tcagctcaaa aattcggtct tgagccagcc gtatttgccg tcgtagttga ctacgaccca
      421 gctgccgtcc tcgttggtgc ccagggcggt cacgccggtc ccggcgggca cgctggtcac
      481 tttgtcgtag tcggtgccgg ggcctgcacg caggttcatg ccggaatcac cgggggtggt
      541 gtagctgggc tgctcgccct cattgtaata ggtggtgggc tgcacactca ggccgtcaaa
      601 ggtgggcacg ggggtcggct ccggggtagg ctccggctca gcgggctgct cttcggcagg
      661 ctgctcctcg gcagggggtt cggcggtgtc gccggtggcc tctgcggtga agctgtcggc
      721 agtcgggtca ttcacgcctg ctgcggcaga ggtggtgtcg ctcgtcgcag gggccgcgtc
      781 accgctgtcg gtcttgccgt caaaagtgcc ggtgagcagc agcacgctga aaatgatgct
      841 ggcaaccagg gccaccgcac agatggccag gcgggtcatg gcttttttgc gtaattcact
      901 catggttaaa cgtcccctta gtggttgaac ttgttttggg cgtactgcaa attgccgtcc
      961 atcagtagtt tgcaggcgtc ctcaatgtcc gggtagcggt cggtcatggc tttgcggtcc
     1021 gcggggggca gcttgcccag cacccaggcg gccagatcgt actgcgggtt tggcttggcc
     1081 ccgatgccga tgcggatacg gttgaagttc tcggtgtcca ggctggcgat gatgcttttc
     1141 agcccattgt gcccgcccgc cgagccgtgg ggccggatgc gcagatgccc cggctcctgg
     1201 gtgatatcgt cacacaggac gatgacgtgg tcggcgggga ttttgtaaaa gtttgcggcg
     1261 ggggcaatgc tctggccgct gaggttcata taggtcagcg gtttgagcag cacgaccttg
     1321 tgctgcccca cctgagcggt gccgtacagg ccctgccact tggcttttgt gatgctgcac
     1381 ccccactgag cggccagata atccagcgct tcaaacccgg cgttgtggcg tgtgccgtcg
     1441 tatttcggtt cggggttgcc cagcccggca atcagccagt ctgccccgga ggtcattcct
     1501 aacatagtgt gtcctttgtc gtgttgatac gctttgccgc ggggcgtcga ggacgctgcc
     1561 ccctgcaata ttatttcgct ttcttttccg cttccagctt ctgctttttg gcgatgtact
     1621 tttccagctt cttctcgccc cagccctcga tctcggcctg cttggcgcgc tcaatgctca
     1681 acgcaccggc gggcacgtcg tggccgatgg tggaaccggc ggcggtgaag gcgtggtcgc
     1741 ccaccgtgac cggcgcgaca aggttcgtat tgcagccgat gaaggcatag tcgccgatgg
     1801 tggtgtggaa cttgccctcg ccatcatagt tgcaggtgac ggtgccgcag ccgaagttgc
     1861 agtacttgcc cacggtggcg tcgccgatgt aggtcaggtg gctgacggtg ttgccctcgg
     1921 caaagtcggc gttcttcgtc tcgacataag cgcccaggtg gcagccttcg gcagtcttgg
     1981 tgccggtgcg tacatgggtg aagggaccga tcttgttgtt ggggccgatg tggctgtcat
     2041 agcactggct ggcgttgatg gtggagcctg cgtccacgac ggtattctcc agcagggtgt
     2101 tggggccgat gacgcagtcc gcgccaatga cggtcttgcc gcgcaggatg cagccgggca
     2161 ggatggtggc accgggggca atgacaacct ccggctcaat atagacatcg cggctgatga
     2221 aattcacgcc gttatccagg tggcgctcaa aggtggcaac gtagcgctgc tgcgtcagtt
     2281 caaatttctc ttttgcggta gacatgagca tcttccttta tatacggttt gcagtcaggg
     2341 gcgggccgct gtgcccgcga aactctgata cccatatatt gtataacacc cggcggattt
     2401 tagcaagccg aaaaacgcct ttcgacgcaa tttcccagaa aaagttctgt gaaagggaaa
     2461 gaatcatgaa aaagtacatc tttttcataa gaaaatttat tatgctttgc agcgggaaat
     2521 gttatactgt agccgtaaga tgcaatagaa gaaggagaga acccccatga aaccccgaaa
     2581 caccctgatg cagaaaatcg cagcgctggc cctggccggt atggcggtaa catactacaa
     2641 aaagaaaacc agatgaaaaa agggaacgga caccgcatcc gttccctttt gtatttacca
     2701 caaa
//
LOCUS       DBMDSQ010000270         2702 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_8388_length_2702_cov_1.600923, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000270 DBMDSQ010000000
VERSION     DBMDSQ010000270.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 2702)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2702)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2702
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8388_length_2702_cov_1.600923"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..676
                     /locus_tag="ACZPAX_08790"
     CDS             <1..676
                     /locus_tag="ACZPAX_08790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1114033.1"
                     /translation="STQRENQKNANIWQLKCHDLQQQVDELQKQLASTRQLNTTAAPA
                     TPTSDLVREAKLEAGKILADARLYADSAEKELKQQADLQKSRMAENARSIASGVMLVR
                     ERLARVDERLSAATLDLDGLTQAIYQALDQTEAELKELGTDMRSFAQGTPETDIPVSA
                     PRPAAKAAAPAGDKPAERLRVKAKAQPVQHKPAAPAPVHRLRNTRRPVSQLLQDQIDR
                     LNGDDQ"
     gene            1093..>2702
                     /locus_tag="ACZPAX_08795"
     CDS             1093..>2702
                     /locus_tag="ACZPAX_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007045847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HIR1114034.1"
                     /translation="MRVGLDIGSTTIKCVVLNDADQIIYSTYERHFSHILEKGKALLE
                     KVAAEYLPDGRAYLAISGSAGMGLADSCKVPFVQEVFATRVAANRLAPGTDCIIELGG
                     EDAKILFLTNGTEVRMNGSCAGGTGAFIDQMATLLKMGADEMDKAAQGATRTYTIASR
                     CGVFAKSDIQPLINQGAQAGDIAASIYQAVVNQTIAGLAQGRPIKGNILYLGGPLTFA
                     KTLRESFDRTLGVTGTLPENSLLFVAMGAAFYADEESDLREVAKRLDDYSATATYVSL
                     PPLFANKQAYEEFHARHLKATVPCLPFGADCGPVHIGIDSGSTTIKLVVIDNDANILF
                     ESYRPNLGNPIPLVKETLLKLYQEHPGLQIASVTTTGYGEELVKNAFHCDRGLVETVA
                     HFTAAKHFLPDVDFIIDIGGQDMKCFKIEDGAISNIFLNEACSSGCGSFLQTFAQALG
                     YDVKEFAALGLFADKPVDLGSRCTVFMNSSVKQAQKDGASIENISAGLSISVVKNALY
                     KVIRASSPEELGRNIVVQGGTFYNEAVLR"
BASE COUNT          566 a          856 c          757 g          523 t
ORIGIN      
        1 gtccacccag cgcgaaaacc agaagaacgc caacatctgg cagctgaaat gccacgactt
       61 gcagcagcag gtggacgagc tgcaaaagca gctggcttcg acccggcagc tgaacactac
      121 cgctgccccc gctacgccca cgtccgattt ggtgcgggaa gccaagctgg aagccggtaa
      181 gattctggcc gacgcccgcc tgtatgcgga cagcgccgaa aaagaactga agcagcaggc
      241 cgatttgcag aaaagccgca tggccgagaa cgcccgcagc attgcgtcgg gcgtcatgct
      301 tgtgcgggag cgcctggccc gcgtggacga gcgtttgagc gctgccacgc tggatctgga
      361 cggcctgacc caggccatct accaggcact ggatcagacc gaggccgagc tgaaagagct
      421 gggcaccgat atgcgcagct ttgcccaggg caccccggag acggatatcc ccgtcagtgc
      481 cccacgcccg gctgccaagg ctgccgcccc ggcaggggac aagcccgccg agcgcctgcg
      541 cgtgaaggca aaggcccaac cggtgcagca taagcccgct gcccccgccc cggttcaccg
      601 cctgcgcaac acccggcggc ctgtgtcgca gctgttgcag gatcagattg atcggctgaa
      661 tggggacgat cagtaaataa agtacatgac cacctggcat aaagccaggt ggtttttgct
      721 gtaagagcct cccgtttgtt cacaaaccct tcttaatttg ttgaattttc cttcacgact
      781 ttgacacatt tgccggatat actataagtg ttctccggga agggagcagg gacccggccc
      841 cccagaacag gaccccaagt ttttcatcca tactcctctt ttcttttgac gcgaaagcgg
      901 tgcggatcgt ctccgcaccg ctttcgtgtt ttttttgtac aaattttcgc atttgacaac
      961 gttttttcgg cgccgcgccc cttgccgaat gccgccgaaa cctgtataat ataagctgat
     1021 attgtatcgc catggggcag acccggccta cggtatcccg cagacactca tacccgcagg
     1081 aggcaaaatc ctatgagagt tggtctggac attggttcca ccacgattaa atgtgtcgtg
     1141 ctgaacgacg ccgatcaaat catctattct acctacgaac gtcatttcag ccatatttta
     1201 gagaagggca aagccctttt ggaaaaggtc gcggcagagt acctgcccga cggccgcgca
     1261 tatttggcta tctccggctc ggccggcatg ggcctggccg acagctgtaa ggtgcccttt
     1321 gtgcaggagg ttttcgccac ccgcgtggca gccaaccgcc tggcccccgg caccgactgc
     1381 atcattgaac tgggcggcga ggacgcaaaa atcctgttcc tgaccaacgg caccgaggtc
     1441 cgcatgaacg gcagctgtgc cggcggcacc ggcgcgttca tcgaccagat ggctaccctg
     1501 ctgaagatgg gtgccgacga gatggacaag gccgcccagg gcgcgacccg cacctacacc
     1561 atcgccagcc gctgcggcgt gtttgccaaa agcgacatcc agcccctcat caaccaaggt
     1621 gcgcaggcgg gcgacatcgc ggcgtccatc tatcaggccg tcgtcaacca gaccattgcg
     1681 ggcctggccc agggccgccc catcaagggc aacattctgt acctcggcgg tccgctgact
     1741 ttcgccaaga ccctgcgcga gagctttgac cgcacgctgg gcgtcaccgg cactctgccg
     1801 gagaacagcc ttctcttcgt cgccatgggc gctgcgttct acgccgacga ggaatccgat
     1861 ttgcgcgagg ttgccaagcg cctggacgat tacagtgcca cggccaccta cgtcagcctg
     1921 ccgccgctgt ttgccaataa gcaggcgtac gaggaattcc acgcccgcca cctgaaagcc
     1981 accgtgccct gcctgccctt tggtgcagac tgtgggccgg tacatatcgg cattgactcc
     2041 ggctccacca ccatcaagct ggtggtcatc gacaacgacg ccaacattct gttcgagagc
     2101 taccgtccga acctgggcaa ccccatcccg ctggtcaaag agacgctgct caaactctat
     2161 caggagcacc cgggcctgca aattgccagc gtgaccacca ccggctacgg cgaggaactc
     2221 gtcaagaatg cgttccactg tgaccgcgga ctggttgaga cggtggccca cttcaccgcc
     2281 gctaagcact ttttgcccga tgtcgatttc atcatcgaca tcggcggcca ggacatgaag
     2341 tgcttcaaga tcgaggatgg tgccatcagc aacatcttcc tgaacgaggc ctgttcctcc
     2401 ggctgcggca gctttttaca gacgtttgcc caggcgctgg gctacgatgt caaggagttc
     2461 gccgcgctgg gcctgtttgc cgacaagccg gtcgatttgg gttcccgctg caccgtgttc
     2521 atgaactcct ctgtcaagca agcacagaag gacggtgcat cgattgaaaa tatcagcgcc
     2581 ggattgtcga tttccgtcgt caagaatgcg ctgtacaagg tcatccgcgc gtccagcccg
     2641 gaagagctgg gccgtaacat cgtcgtgcag ggcggtactt tctataatga agctgtcctc
     2701 cg
//
LOCUS       DBMDSQ010000271         2698 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_8404_length_2698_cov_1.693107, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000271 DBMDSQ010000000
VERSION     DBMDSQ010000271.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 2698)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2698)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2698
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8404_length_2698_cov_1.693107"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2698)
                     /gene="rpoB"
                     /locus_tag="ACZPAX_08800"
     CDS             complement(<1..>2698)
                     /gene="rpoB"
                     /locus_tag="ACZPAX_08800"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005926187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HIR1114035.1"
                     /translation="TITDNVSSLHEVFHDIAAIEDYTGNLVLEFVDYRLDKNPKYSIK
                     ECKERDATYAAPLYVTARLFNKQTGEVKEQEIFMGDFPLMTDAGTFISNGAERAIVSQ
                     LVRSPGVFYGSSKDRTGKDLFTATMNPNRGAWLEYETDSSDVYYVRIDKNRKLPVTTL
                     LRALGLSTDEQIKQFFGDSEPKINASLEKDITHNTEEGLLEVYRKLRPGEPPTVENSR
                     AHLNNLFFDPRRYDLARFGRYKMNNKLSLTRRIAGYKAAEDIIAPLTGELLAAKGEKI
                     NMAKAEEIDNAGVTRVVILVEKKGEEPRPFIVISNGCVNAQNFFSFDVEAEAGVNERA
                     NFAEIRKILDTTSDVEEQKELLRQNHDVLISRTVTVDDIFASVNYLLGLDHGIGTTDE
                     IDHLGNRRVRSVGELLQNQFRIGFSRMERVIRERMTLQNQENGEITPQSLVNIRPVVA
                     AIKEFIGSSPLSQFMDQNNPLAELTHKRRLSALGPGGLSRDRAGFEVRDVHYTHYGRL
                     CPIETPEGPNIGLISYLATYAKINKYGFVEAPYRKVDKATGTVTDEVIYMTADEEDEY
                     IVAQANEPLDENNHFVRTRVSGRHRNDIQEFEASQVDFMDVSPRMMVSVATACIPFLE
                     NDDCNRALMGSNMQRQAVPLMVTQQPLVATGMEYKAATDSGVCVLAAHDGTVEYVDAE
                     KVVVRCADGSADTYELIKFMRSNQGNCNNQRPIVNVGETVKAGDVLADGPATRNGEIS
                     LGKNALVGFMTWEGYNYEDAVLLNEKLVREDVYTSIHIEEYETEARDTKLGPEEITRD
                     IPNVGDDALKDLDDRGIIRIGAEVKTGDILVGKVTPKGETELTAEERLLRAIFGEKAR
                     EVRDTSLRVPHGAYGIVVDVKVFTPENSDELQPGVRMCVRCY"
BASE COUNT          493 a          752 c          882 g          571 t
ORIGIN      
        1 atgtagcagc gcacgcacat acgcacgccg ggctgcagtt cgtcgctgtt ctcgggggta
       61 aagaccttga cgtcaacaac gatgccgtaa gcgccgtggg gcacgcgcag ggaggtgtca
      121 cggacttcgc gggccttctc gccgaagatg gcgcgcagca ggcgttcctc ggcagtcagc
      181 tcggtctcgc ccttcggggt gaccttgccg acgaggatgt cgccggtctt gacctcagca
      241 ccgatgcgga tgatgccgcg gtcgtccaga tctttcagag cgtcgtcgcc gacgttcggg
      301 atatcgcggg tgatttcctc aggtccgagc ttggtgtcgc gggcctcggt ctcgtactct
      361 tcgatatgga tggaggtgta gacatcctcg cggacgagct tctcgttcag cagaacggcg
      421 tcctcgtagt tatagccttc ccaggtcatg aagccgacca gggcgttctt gcccaggctg
      481 atctcgccgt tgcgggtcgc ggggccgtcc gccagcacgt cgccggcctt gactgtctcg
      541 cccacgttga cgatggggcg ttggttgttg cagttgccct ggttggagcg catgaacttg
      601 atgagttcgt aggtatcggc agagccgtct gcgcagcgga caacgacctt ttcagcatcc
      661 acatactcaa ccgtgccgtc gtgggcggcc aggacgcaga caccggagtc ggtggcagcc
      721 ttgtactcca tgccggtggc gaccagcggc tgctgggtga ccatcagagg cacggcctgg
      781 cgctgcatgt tggagcccat cagagcacgg ttacagtcat cattctccag gaacggaatg
      841 caggccgtag cgacggaaac catcatgcgg ggagaaacat ccataaagtc gacctgagag
      901 gcctcaaact cctggatgtc gttgcggtga cggccggaaa cgcgggtacg gacgaagtgg
      961 ttgttctcgt ccagcggctc gttggcctgg gcgacgatgt actcgtcctc ctcgtcggcg
     1021 gtcatataga taacttcatc ggtgacggtg ccggtagcct tgtccacctt gcggtagggg
     1081 gcctcgacaa agccgtactt gttgatcttg gcgtaggtag ccaggtagga aatcagaccg
     1141 atgttcgggc cttcaggcgt ctcgatgggg cacagacggc catagtgggt gtaatgaacg
     1201 tcgcggacct caaagcctgc gcggtcacgg gacagaccgc cggggcccag agcggacaga
     1261 cgacgcttgt gggtcagctc ggccaggggg ttgttctggt ccatgaactg ggacagcggg
     1321 ctggaaccga tgaactcctt gatggcggcg acgaccgggc ggatgttgac caagctctgc
     1381 ggggtgattt cgccgttctc ctggttctgc agggtcatgc gctcacggat gacgcgctcc
     1441 atgcgggaga agccgatgcg gaactggttc tgcagcagct caccgacgct gcggacgcgg
     1501 cggttgccca ggtggtcgat ctcatcggtg gtgccgatgc cgtgatccag gcccagcagg
     1561 tagttgacgg aagcgaagat gtcatcgacg gtgacggtgc ggctgatcag aacatcgtgg
     1621 ttctggcgca gcagctcctt ctgctcttcc acgtcggagg tggtgtcgag gatcttgcgg
     1681 atctcggcga agttggcacg ctcgttgaca ccggcctcgg cctcaacgtc aaaactgaag
     1741 aaattctggg cgttgacgca gccgttggaa atgacgatga acggacgcgg ctcctcgccc
     1801 ttcttctcga ctaggataac aacgcgggtg acaccggcgt tgtcgatttc ctcagccttg
     1861 gccatgttga tcttctcgcc cttggcggcc agcagctcgc cggtcagggg ggcgatgata
     1921 tcctcagcag ccttgtagcc ggcaatgcga cgggtcaggc tgagcttgtt gttcatcttg
     1981 tagcggccga aacgagccag gtcgtagcgg cgcgggtcga agaacaggtt gttcagatgg
     2041 gcgcgggagt tctcgaccgt cgggggctca ccggggcgca gcttgcggta aacctccagc
     2101 aggccttcct cggtgttgtg ggtgatgtcc ttctcgagag aggcgttgat cttcggctca
     2161 gagtcgccga agaactgctt gatctgctca tcggtggaca ggcccagggc acgcagcagc
     2221 gtggtgaccg gcagcttgcg gttcttatcg atgcggacat agtacacatc ggaggaatcc
     2281 gtctcatact ccagccatgc gccgcggttg gggttcatgg tggcggtaaa caggtccttg
     2341 ccggtacggt ccttgctgga gccatagaaa acaccggggg aacggaccag ctgagagacg
     2401 atcgcacgct ctgcaccgtt ggaaataaag gtgccggcat cggtcatgag cgggaagtcg
     2461 cccatgaaaa tctcctgctc cttcacctca ccggtctgct tgttgaacag acgggcagtg
     2521 acgtacaggg gtgccgcata cgtggcatcc cgctctttac attctttaat agaatatttg
     2581 gggttcttgt ccagccgata atcaacgaac tccagcacca ggttgccggt gtagtcctcg
     2641 atcgcggcaa tgtcgtggaa aacctcgtgc aggctactga cattatcggt tatcgtat
//
LOCUS       DBMDSQ010000272         2684 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_8464_length_2684_cov_2.077816, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000272 DBMDSQ010000000
VERSION     DBMDSQ010000272.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 2684)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2684)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2684
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8464_length_2684_cov_2.077816"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            28..807
                     /locus_tag="ACZPAX_08805"
     CDS             28..807
                     /locus_tag="ACZPAX_08805"
                     /inference="COORDINATES: protein motif:HMM:NF014582.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HIR1114036.1"
                     /translation="MQTSPIVLTTIAGLSTGLGGALAVLCTPTERLLAASAGFAGGVM
                     LTASLTDLLPEALDFYAAYLPPLGSGGAAVSLLALGMLTAALLGRLLPEESELADRFG
                     QGRDPARAAAMRTALITGAALLLHNFPEGVLTFFAGTADPALGLRTAAAIALHNIPEG
                     LAVAVPFAYATRSRAAGVLAALVSGLAEPLGAVLAWLFLRRFFTPGFLTGTVVLAAGI
                     MVWVALAQLLPQAMAAKHRAAGIFGAAAGTLLMLLGIAALP"
     gene            857..2158
                     /locus_tag="ACZPAX_08810"
     CDS             857..2158
                     /locus_tag="ACZPAX_08810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1114037.1"
                     /translation="MMKKITALLFVPLLLAGCSAEGNVETLLRAPQLSGESAALQKAL
                     NSYLGGSATLKYPANGDFLSPFAFGDWDGDGTDEAAILYTTDTTSSNVWLAVLEPSGD
                     SGWRVSHAIEGLTSEVQSFSAAHLRDTTSQQLLAGYISPQGDQYLVVYQYDGATLSTV
                     ISKSYTDMIVADFTGKGDTQDLVLALPPDSELGGVTLQLLTANDGEFRSTQTLNLGEG
                     VYSSCAALHAGTGSDDGMYLVMDAWTGASGLVSDIILYDAETGFLQPYRPSGLSDIQR
                     STLRYHSELLSCDIDNNGTVDIPVEIDDGGTLQTPMDKRLSFALWKDYTSAAGGNSRF
                     GVYDSEYNFFMQLPESMHGSVLIRSNQSGTGWLLCNAEGTTVYCEMRVVDPADNAATG
                     VGNYLRIANIGSQQLQARVITPYYGLSLDTISQNTVLLGSN"
     gene            2181..>2684
                     /locus_tag="ACZPAX_08815"
     CDS             2181..>2684
                     /locus_tag="ACZPAX_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR1114038.1"
                     /translation="MKRVLLVEDEASIREMVALNLKMAGWEVVEAPSAERALELMHGG
                     ERCDAALLDIMLPGMDGLSLCETIRRDDSDIGIIIVSAKGQESDKIRGLSIGADDYIT
                     KPFSVSELIARLEALTRRIHRGSSGSVPKPTEEPEQLVSGPFVLDEKSRILYKSGTPI
                     DLTQVEFQ"
BASE COUNT          496 a          822 c          882 g          484 t
ORIGIN      
        1 attgccctaa agattggggg cgtacctatg caaacttcac cgattgtact taccacgatt
       61 gcggggctgt ccacgggatt gggaggggcg ctggcggtgc tctgcacacc gacagaacga
      121 ctgctggcgg ccagcgcagg ctttgcggga ggggtcatgc tcacggcgtc gctgaccgat
      181 ctgctgcccg aagcgctgga tttttacgcc gcttacctgc cgccgctggg cagcggcggg
      241 gcggcggtgt cgctgctggc actgggaatg ctgaccgctg cgctgctggg acggcttttg
      301 cccgaggaat ccgagctggc cgaccgcttt ggtcagggcc gcgacccggc ccgcgcggcg
      361 gccatgcgca cggccctcat cactggcgcg gcactgctgc tgcacaactt ccccgagggc
      421 gtactcacat tcttcgccgg gacggcagac ccggcgctgg gtctgcgcac tgcggcggcc
      481 attgcactgc acaacatccc ggaggggctt gccgtggcgg tgccctttgc ctacgcgacc
      541 cgcagccgcg cagccggtgt gctggcggcg cttgtctccg ggctggcgga acccttggga
      601 gcggtgctgg cctggctgtt cctgcgccgt ttttttacac ccggcttttt gacggggacg
      661 gtcgtgctgg cggcgggtat tatggtatgg gtggccctgg cccagctgct gccccaggcc
      721 atggcagcca aacatcgggc ggcgggcatt tttggtgcgg cggcaggcac cctgctgatg
      781 ctgttaggca ttgcggcgct gccgtaaatg tggtatacta tattcgtata tttatgtgag
      841 aaaggagcag accgccatga tgaaaaaaat caccgcgctg ctctttgtgc cgcttttact
      901 ggcggggtgc agcgcggagg ggaacgtgga gaccctgctg cgcgcgccgc agctttccgg
      961 tgagagcgcg gccctgcaaa aggcgctgaa cagctatctg ggcggcagcg ccaccctgaa
     1021 ataccccgcc aacggtgact tcctctcgcc tttcgccttc ggcgactggg acggcgatgg
     1081 caccgacgag gcagccatcc tctacaccac cgacacgacc agctccaacg tctggctggc
     1141 tgtgctggag cccagcggcg acagcggctg gcgcgtcagc cacgccatcg agggcctgac
     1201 cagcgaggtg cagagctttt ctgccgcgca cctgcgggac accaccagcc agcagctgtt
     1261 ggccgggtac atctcgcccc agggggatca atatctggtg gtctaccaat atgacggtgc
     1321 tacgctgtct acggtcataa gcaagagcta taccgacatg atcgtcgccg acttcaccgg
     1381 caagggcgac acgcaggatc ttgtgctggc cctgccgccg gacagcgaac tgggcggcgt
     1441 gacgctgcaa ttactgaccg ccaacgatgg cgagttccgc tcgacccaaa cgctgaacct
     1501 gggcgagggc gtgtacagca gctgcgcagc tctgcacgcg ggcaccggca gcgacgacgg
     1561 tatgtacctg gtcatggacg cctggaccgg ggccagcggc ctggtgtcgg acattatttt
     1621 gtacgacgcc gagaccggct ttttgcagcc ctaccgcccc agcggtttga gcgacatcca
     1681 gcgcagcacc ctgcgctacc acagcgaatt gttgagttgc gacattgata acaacggcac
     1741 cgtggacatc ccggtggaga tcgacgacgg cggtacgctg caaacgccga tggacaagcg
     1801 cctgagtttt gcgctctgga aggattacac ctcggcggcc ggcggcaaca gccggtttgg
     1861 cgtgtacgac agcgagtaca actttttcat gcagctgccg gagtcaatgc acggcagtgt
     1921 gctgatacgc tcgaaccaga gcggcacggg ctggctgctc tgcaacgccg agggcacgac
     1981 agtctactgc gaaatgcggg tcgtggaccc ggccgacaac gcggccaccg gcgtggggaa
     2041 ctacctgcgc attgccaaca tcggcagcca gcagctgcag gcgcgggtga tcacacccta
     2101 ttacggattg agtttggaca ccatcagcca gaacaccgtg ctgttgggtt ccaattaaaa
     2161 taaggaaggg aacctgttgt atgaaacgag ttttactggt cgaggacgaa gccagcatcc
     2221 gcgaaatggt agccctgaac ctgaaaatgg cgggctggga agtggtggaa gcccccagcg
     2281 ccgagcgtgc gctggagctg atgcacggcg gcgagcgctg cgatgcggcg ctgctggaca
     2341 ttatgctgcc gggcatggac ggcctgagcc tgtgcgaaac catccgccgc gacgacagcg
     2401 atatcggcat catcattgtg tcggccaaag ggcaggagag cgacaagatc cgcggcctgt
     2461 ccatcggcgc ggacgattac ataaccaagc cattttccgt gtcggagctg attgcccgcc
     2521 tggaggcact gacccggcgc attcaccgcg gcagcagcgg cagtgtgccc aaacctaccg
     2581 aggagccgga gcagctggtc agcgggccgt tcgtgctgga cgaaaagagc cgcattctgt
     2641 acaagtccgg cacgcccatc gacctgaccc aagtggagtt ccag
//
LOCUS       DBMDSQ010000273         2651 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_8595_length_2651_cov_1.660784, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000273 DBMDSQ010000000
VERSION     DBMDSQ010000273.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 2651)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2651)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2651
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8595_length_2651_cov_1.660784"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(91..2157)
                     /locus_tag="ACZPAX_08820"
     CDS             complement(91..2157)
                     /locus_tag="ACZPAX_08820"
                     /inference="COORDINATES: protein motif:HMM:NF039722.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6311 domain-containing protein"
                     /protein_id="HIR1114039.1"
                     /translation="MKTLKKYRWPLTGALLGALIFLLLYGVRVLDPTSVDWILNNPSP
                     DPAQHYLGWELFRRSPVHLPYIGANYNAVYPFRTSVLFTDSLPLAALLFKLLGGILPT
                     RFQYFGWWGLFCYMMQGGLAQAVIARIAGVQPTFGRGDKSKAAIAIIMSPGQTAKLWG
                     SVLGAGVLVLFPALTMRTFAHEALAGTWLVLLALYLWLRSDELMSATRRACLLWGLMG
                     LLCAGIHLYYLPMVGLVLAGYAVRRALQKRGPAAVLLPIAAFCAAALAELFLLGAFAV
                     NFAGYSNGYLSGADYLGLFVPWLAPSWEQNVYMGLGAVLAVVLAIFSVVCNARKAERF
                     FTAHHDWVVAGVVVLVLDLLAAGGNAITVGGKTLFTVPIPQFLMDFWAMFSSCARLAW
                     VAGLLLAVAACGLVLRFWQKGVVPALMLAVCAVAQGWGQRSELFNRWTDYHYYGFRYE
                     NKTLLTDPVWEQIAASGRYSHLAFATFDFEHDEFWDLVDFAADHGWTSNSFYMAHMDG
                     NLAAVTLPGELNELSADTLYAFIDEDELARNSYDLHYYRLDGILIGSVEPIDGLEEEP
                     APEVPAHTMDLTKSDVDPLFLAADGIVLGMNGEMMTEEWTLFPGRYRVTLTGSGFDHS
                     YIYARYGLINEETYKLDIDFTGIDPNEMTFEFTATEMLHYWRVAVHTLDDANVTVNSV
                     TVEKIV"
     gene            complement(2170..>2651)
                     /locus_tag="ACZPAX_08825"
     CDS             complement(2170..>2651)
                     /locus_tag="ACZPAX_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005922036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HIR1114040.1"
                     /translation="ELRFGIGYHPAFAFPFDDRHVTEDYEIRFDGIESPLCVSAQPNG
                     LLNGESYYLARNVEAIPLTDDLFDNDSHAMVNLRSGHVSVIEKDTGRSVICDVSDFPY
                     TLIWSAAKKPLHFICIEPWHSLPGEENGPLEWEQRPCAASLKRGESWSTTLSTTFVR"
BASE COUNT          572 a          800 c          816 g          463 t
ORIGIN      
        1 ttgccaaaaa ggttgcgttt taagtaagta tttaagtaag taccgtataa aaaactgcct
       61 gcaaatggaa tcgcatttgc aggcagtttt ttatacgatt ttttctaccg tcacactgtt
      121 gacggtgaca ttggcatcat ccagtgtgtg gacggcaacg cgccagtagt ggagcatctc
      181 ggtggccgtg aactcaaagg tcatttcgtt ggggtcgatg ccggtgaagt cgatgtccag
      241 cttgtaggtt tcttcgttga tgaggccgta ccgcgcgtag atgtagctgt ggtcaaagcc
      301 gctgccggtc agcgtcacac ggtagcgccc ggggaacagc gtccactcct cggtcatcat
      361 ctcaccgttc atgcccagaa caatgccgtc cgccgccaga aagagcgggt caacatccga
      421 cttcgtcaaa tccatcgtgt gggcgggcac ttcgggcgca ggctcctcct ccaggccgtc
      481 gatgggctcg acgctaccga tcaggatgcc atccagccgg taatagtgca ggtcgtaact
      541 gttccgcgcc agctcgtcct cgtcgatgaa ggcatagagc gtgtcagcgg aaagctcgtt
      601 cagctcgccc ggcagcgtga ccgccgcaag gttgccgtcc atatgcgcca tgtagaagct
      661 attggaggtc cagccgtggt cggcggcaaa gtcgaccaga tcccagaact cgtcatgctc
      721 aaagtcaaat gtggcaaagg ccagatggct gtacctgccg ctggcggcga tctgctccca
      781 tacggggtcg gtcagcaggg tcttattctc gtagcggaag ccgtagtagt ggtagtccgt
      841 ccagcggttg aacagttccg accgctggcc ccagccctgc gccacagcgc agacggccag
      901 catcaaagcg ggtacaacgc ccttttgcca aaagcgcagc accaggccgc aggcggcgac
      961 agccagcagc aggcccgcca cccacgccaa acgcgcacag gaggaaaaca tcgcccaaaa
     1021 gtccatcaag aactgcggaa tgggcacggt gaacagcgtc ttgccgccca ccgtaatggc
     1081 gttgccgccc gcggccagca ggtcgagtac aagcacgaca acacccgcca cgacccagtc
     1141 atggtgcgcg gtgaagaacc tctccgcctt gcgcgcattg cagaccacgc taaaaatcgc
     1201 cagcacaaca gccagcactg cgccgaggcc catgtagaca ttctgctccc agcttggcgc
     1261 gagccacggc acaaacaagc cgaggtagtc cgcaccgctt aaatatccgt tggagtagcc
     1321 tgcaaagttg acggcaaacg cgccgagcag gaatagctca gccagcgccg ccgcgcagaa
     1381 cgcggcaatg ggcagcagca ccgcagccgg gccgcgcttt tgcagggcgc ggcgcaccgc
     1441 gtaccctgcc agcaccagcc cgaccatggg caggtaatac agatggatgc ccgcgcagag
     1501 cagccccatc agcccccaga gcagacaggc gcggcgggtc gcagacatga gttcgtcgct
     1561 gcgcagccag aggtagagcg ccagcaatac aagccaggtt ccggccagcg cctcatgggc
     1621 gaaagtgcgc atcgtcagcg ccgggaagag cacaagcacc cccgcaccca acacactgcc
     1681 ccacagtttg gcggtctggc cggggctcat gatgatggcg atggcggcct tgctcttgtc
     1741 gccccggcca aatgtgggct gcacgcccgc gatgcgggca atgacggcct gcgccagccc
     1801 gccctgcatc atgtagcaga acagccccca ccagccaaaa tattggaacc gggtgggcag
     1861 gatgccaccc agcagcttaa acagcagcgc cgccagcggc agtgaatcgg taaacaggac
     1921 gctggtgcgg aagggataga ctgcgttgta gttcgcgccg atatacggca gatgtaccgg
     1981 gctgcggcgg aacagctccc acccgaggta gtgctgcgcg gggtcggggc tggggttgtt
     2041 caagatccag tcaacgctgg tggggtccag cacccgcaca ccgtacagca gcaaaaagat
     2101 cagcgctccc agcagcgcac cggtgagggg ccagcgatat tttttcagtg ttttcatctt
     2161 tgccaatttt taccgcacaa acgtcgtgga cagggtggtg gaccagcttt caccgcgctt
     2221 gagggatgcg gcgcaggggc gctgctccca ttcgagggga ccgttttcct cgccgggcag
     2281 actgtgccaa ggctcgatgc agataaagtg cagcggcttt ttggcagcag accaaatcag
     2341 cgtgtagggg aagtccgaca catcgcagat gacgctgcgg ccggtgtcct tctcgatgac
     2401 tgagacatgg ccgctgcgca ggttgaccat agcgtgactg tcgttgtcaa acaaatcgtc
     2461 cgtcaggggg atggcctcga cgttgcgggc gagataatag ctctcgccgt tgagcaggcc
     2521 gttgggctgg gcgctgacgc agagcggcga ctcgatgccg tcaaagcgaa tctcgtagtc
     2581 ctcggtgaca tggcggtcat cgaaggggaa ggcgaacgcc gggtggtagc cgatgccgaa
     2641 gcgcagttct t
//
LOCUS       DBMDSQ010000274         2650 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_8597_length_2650_cov_1.849353, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000274 DBMDSQ010000000
VERSION     DBMDSQ010000274.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2650)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8597_length_2650_cov_1.849353"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="ACZPAX_08830"
     CDS             complement(<1..252)
                     /locus_tag="ACZPAX_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015537136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HIR1114041.1"
                     /translation="MKHTDSITKLNLEQKCALLSGDTVFTTRGYKNAGVPSITLSDGP
                     NGVRKQAGAADHLGLNPSVPATCFPTAATVACSWDPALGE"
     gene            complement(322..>1551)
                     /locus_tag="ACZPAX_08835"
     CDS             complement(322..>1551)
                     /locus_tag="ACZPAX_08835"
                     /inference="COORDINATES: protein motif:HMM:NF013126.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HIR1114042.1"
                     /translation="PKANTPTRAMTDNVKVLNLYTADDANGYINDADETITTGAKNDM
                     KIEDNGKVTELGLQLGSDFNDPQWDALLDELTIDEMENLYINAYGGLAELKSVGKQKS
                     KDADGPAQIGGFTGMGAGTGFPSSSTLAQTWNADLAQEEGRTIGTQALQNGYTGWYAP
                     ATNMHRSPFNGRNYEYYSEDSLLSGIICGNTVTGANQAGVYTYVKHFICNDGESGIYR
                     DSVYTWMTEQALRETYLRPFQMLVEDYDAVGLMSSYNRIGAVWAGGSEALLTGILRDE
                     WGFDGAVITDYCDHHSYMNGDQALRAGGSLWMAGFTGGEMAYETGSNSYMQALRRATK
                     ETLYMYLHVRVTNQAYADSIGDTSVVRHNFTASVFGWRHLVALIDIVAVLLFALAVRG
                     VVIDVKLRKAAKAEKKN"
     assembly_gap    1552..1561
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1562..>2650)
                     /locus_tag="ACZPAX_08840"
     CDS             complement(<1562..>2650)
                     /locus_tag="ACZPAX_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015564569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HIR1114043.1"
                     /translation="IVIDDTRTMLELTTEEENLLNYVGANYAHVVVLINSTNVMELGQ
                     IETIPGIDSCLIVGLSGSEGATAVPEVLWGDKEPSGRTADTWAYDLTTAASYANAGLE
                     GVGKYSNADGLYPADGTTTGNLDTPYAYEQVSYVDYAEGIYIGYKWYETADAEGYWDA
                     ESNEHGTGYDAVVQYPFGYGLSYTSFDWEVTDASANGSTLTKDGNVTVKVTVTNTGER
                     AGKDVVELYYTAPYIAGEIEKSSVELGAFAKTKELQPGESEEVTLTVPVSDMASYDAY
                     DANHNNFTGYELDAGDYIFTVRHNAHDVDAAANATITCNLPSGIQYAEDTVTGNVVSN
                     KFTGSDAIDGVSLDGSDSEQNITYMTRA"
BASE COUNT          464 a          740 c          844 g          592 t
ORIGIN      
        1 ctcgcccagc gccggatccc agctgcacgc cacggtggcg gctgtaggga agcaggtggc
       61 cggcacgctg gggttcagcc ccaggtgatc cgccgccccg gcctgcttgc gcacgccgtt
      121 ggggccgtcg gacagggtga tggacggcac gcccgcgttt ttgtagcccc gcgtcgtgaa
      181 tacggtatca ccggacagca gagcgcattt ttgttcaagg tttagtttgg ttatactatc
      241 tgtgtgtttc atggggtagt cactccttac acataacagt gtgggcgcac acgcaggtgt
      301 gcccgcattg cccacatggg gttagttttt cttttcagcc ttggccgcct tgcgcagctt
      361 gacgtcaatg acgacgccgc ggacagccag tgcgaagagc agaacagcca caatgtcgat
      421 cagggcgaca aggtgacgcc agccgaacac agaagccgtg aagttgtggc gaacaacgct
      481 ggtatcgccg atgctgtcag cataagcctg gttggtcaca cggacatgca ggtacatata
      541 cagcgtctcc ttggtggcgc ggcgcaaagc ctgcatatag ctgttggagc cggtctcata
      601 ggccatctcg ccgccggtga agccagccat ccacaggctg ccgcctgcgc gcagggcctg
      661 gtcgccgttc atgtagctgt ggtggtcgca gtagtcagtg atgaccgcgc cgtcaaagcc
      721 ccactcatcg cgcaggatgc cggtcagcag cgcctcgctg ccgcctgccc agacagcgcc
      781 gatacggttg tagctgctca tgaggccgac cgcgtcataa tcctcgacca gcatctggaa
      841 ggggcgcagg taggtctcgc gcagagcctg ctcggtcatc caggtgtaga cagagtcacg
      901 gtagatgccg ctctcgccat cgttgcagat gaagtgcttg acgtaggtgt aaacgcctgc
      961 ctggtttgcg ccggtcacag tgttgccgca gatgatgccg gacagcaggc tgtcctcaga
     1021 ataatactcg tagttacgtc cattgaaggg gctgcggtgc atgttggtgg caggggcata
     1081 ccagccggtg taaccgttct ggagtgcctg ggtgccgatg gtgcggcctt cctcctgggc
     1141 aaggtctgca ttccaggtct gggccagcgt gctggagctg gggaaaccgg tgccagcacc
     1201 catgccggtg aagccgccga tctgggcagg gccgtcagcg tctttgcttt tttgcttgcc
     1261 gacactcttc agctcggcca gaccgccgta agcgttgatg tacaggtttt ccatctcgtc
     1321 aatggtcagt tcgtccagca gagcgtccca ctgggggtcg ttgaagtcgg aaccaagctg
     1381 caagccaagc tcggtgacct taccgttgtc ctcaattttc atgtcgttct ttgcgccggt
     1441 ggtgatagtc tcatcggcgt cgttgatgta gccgttggca tcatcagcag tatagaggtt
     1501 cagcaccttg acgttgtcgg tcatggcgcg ggtcggggtg ttggccttgg gnnnnnnnnn
     1561 ncagcacggg tcatgtaggt gatgttctgc tcagagtcgc tgccatccag gctcacgccg
     1621 tcgatagcat cgctgccagt gaacttgttg gaaacgacgt tgccggtgac ggtatcctcg
     1681 gcatactgaa tgccggaggg caggttgcaa gtgatggtgg cgttggcggc ggcgtccacg
     1741 tcatgggcgt tgtggcgtac ggtaaagatg tagtcaccgg cgtccagctc ataaccggta
     1801 aagttgttgt ggttggcgtc gtaggcgtca tagctggcca tgtcagagac agggacagtc
     1861 agcgtgactt cctcgctctc accgggctgc aattccttcg tcttggcgaa agctcccagt
     1921 tcgacactgg atttttcgat ttcgcctgcg atgtagggcg cagtgtagta gagttccaca
     1981 acgtccttgc ccgcgcgttc gccggtgttg gtgacggtga ctttcacggt cacattgccg
     2041 tccttggtca gcgtgctgcc gttggcggag gcatcggtga cctcccagtc aaagctggta
     2101 tagctcaggc cgtagccaaa ggggtactgt acaacggcat catagccggt gccgtgctca
     2161 ttgctctcgg catcccagta gccctcggcg tcggcggtct cgtaccactt gtagccgatg
     2221 taaatgccct cggcatagtc gacgtaggag acctgctcgt aggcgtaagg agtgtccagg
     2281 ttgcctgtgg tggtgccgtc agccgggtac aggccgtcgg cgttgctgta cttgcccacg
     2341 ccctcaaggc cggcgttcgc gtagctggca gcggtggtca ggtcgtaggc ccaggtgtca
     2401 gcggtacggc cgctgggttc tttgtcgccc cacaggactt cgggcacggc ggttgccccc
     2461 tcggagccgg aaagaccgac aatcaggcag gaatcaatgc cagggatcgt ctcgatctgg
     2521 cccagctcca taacgttggt agagttgatg agcacaacga cgtgggcata gttcgcgccg
     2581 acatagttca gcaggttttc ttcctcggtg gtcagttcca gcatcgtgcg ggtgtcgtcg
     2641 ataacaatgt
//
LOCUS       DBMDSQ010000275         2645 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_8626_length_2645_cov_1.260613, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000275 DBMDSQ010000000
VERSION     DBMDSQ010000275.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 2645)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2645)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2645
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8626_length_2645_cov_1.260613"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1385)
                     /gene="asnS"
                     /locus_tag="ACZPAX_08845"
     CDS             complement(<1..1385)
                     /gene="asnS"
                     /locus_tag="ACZPAX_08845"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015537626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HIR1114044.1"
                     /translation="MQRTELASLYTATPADGTKVTVCGWAKTVRDSKNIGFISLSDGS
                     CYKPVQIVFQADKLANYAEIAKCGLYTSFEVTGTLVVTPGTKQPFEINADEITVLGPC
                     GGDYPLQKKKMGMDYLRTMTHLRPRTNTFNAAFRVRGQAAFALHQFFHEHGFTYVHTP
                     IFTGSDCEGAGEMFQVTTLDLNDIPRNDEGGVDYTKDFFKRPVNLTVSGQLEAEAMAM
                     ALGNVYTFGPTFRAEKSYDTRHAAEFWMIEPEMAFADLNTYLETAEAMTRYVIRYLLD
                     NCHDELAFFNQFFDKGLIERLELVASSDFARVTYTDAIELLKKNNDNFQYKVEWGTDL
                     QTEHERYLTEQIFKKPVFVTDYPKEIKAFYMRQNEDGKTVAAADMLVPGIGELIGGSQ
                     REERLDVLEARMKELGLNTADYDWYLDLRRYGSVKHAGYGLGFERLLMYVTGIPNIRD
                     VIPFPRTCGGF"
     gene            complement(1497..>2645)
                     /gene="spoIIP"
                     /locus_tag="ACZPAX_08850"
     CDS             complement(1497..>2645)
                     /gene="spoIIP"
                     /locus_tag="ACZPAX_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061783 - peptidoglycan muralytic activity
                     [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein P"
                     /protein_id="HIR1114045.1"
                     /translation="GVCAMGKLRRWAAAALCGAVFAGGVLAVAAACAACPGWAWLIRG
                     ALFLQDPGAAAAVSWPADAAQTVYKPQRPDRRPTPLPTVTPSPAPTQAPTAEPTPGPT
                     ATPLSSAVPELPKSESAGRIRSVTLKQGSGDGYVQLAAGSIKNSTEHPDADLRAAVTT
                     QSLPFAIEKNSGEPQILIVHTHATESYQTWPDPVFDENYTARSKNTALNMVAVGAEMA
                     RVLNAAGINTLHDTTLYDAPGYTSSYQRSRAGVQDYLARYPSIKIVLDVHRDAIEDSD
                     GTRVKPVCEIDGESTAQVMVIAGCDNGGSAALPNWRLNLRFAAAWEAAMEGAYPGLTR
                     PVLCGYRFYNQDLTTGSLLIEIGGHANTLDEALRAGRYAAEGLVKAVG"
BASE COUNT          462 a          792 c          844 g          547 t
ORIGIN      
        1 caaaaaccac cgcaggtgcg ggggaagggg atgacgtcgc ggatgttggg gatgccggtg
       61 acgtacatca gcaggcgctc aaagcccaag ccgtaaccgg cgtgcttgac actgccataa
      121 cggcgcaggt caagatacca gtcgtagtcg gcggtattca ggcccagttc cttcatgcgg
      181 gcttccagca cgtccaggcg ctcctcacgc tgggagccgc cgatcagctc accgatgccg
      241 ggcaccagca tatcggctgc agcaacagtc ttgccgtcct cgttctggcg catatagaac
      301 gccttgattt ccttcgggta gtcggtgacg aacaccggct tcttgaagat ctgctcggtc
      361 aggtagcgct cgtgctcggt ctgcagatcg gtaccccact cgactttgta ctggaagttg
      421 tcgttgttct tcttcagcag ctcgatggcg tcggtgtagg tcactcgggc aaagtcgctg
      481 ctggcaacca gttccaggcg ctcgatgaga cccttgtcaa agaactggtt gaagaaggcc
      541 agctcgtcgt ggcagttgtc cagcaggtag cggatgacgt agcgggtcat ggcctcggcg
      601 gtctccaggt aggtgttcag atcggcgaaa gccatctccg gctcgatcat ccagaactcg
      661 gcggcgtggc gggtatcgta gctcttctcg gcgcggaagg tagggccgaa ggtatagacg
      721 ttgcccaggg ccattgccat ggcctcggcc tccagctggc cggagacggt caggttgacc
      781 gggcgcttga agaagtcctt cgtatagtca acgccgccct cgtcgttgcg ggggatatcg
      841 ttgaggtcga gggtcgtgac ctggaacatc tcaccggcgc cctcgcagtc agagccggta
      901 aaaatcggcg tatgcacgta ggtaaatccg tgctcatgga agaactgatg cagggcaaac
      961 gccgcctggc cgcgcacgcg gaacgccgcg ttgaaggtgt tggtgcgggg gcgcaggtgg
     1021 gtcatggtgc gcaggtaatc catgcccatt ttctttttct gcagcggata atcaccgccg
     1081 caggggccaa gcaccgtgat ctcgtcggcg ttgatctcaa agggctgctt ggtgcccggg
     1141 gtcacgacca gcgtgccggt gacctcaaag ctcgtgtaca ggccgcactt ggcgatttcg
     1201 gcatagttgg cgagcttgtc agcctggaaa acgatctgga cgggcttata gcagctgccg
     1261 tcggacagcg agataaagcc gatattttta gaatcgcgca cagttttggc ccagccgcag
     1321 acagtgacct tggtaccatc ggcaggggta gcggtgtaca gcgatgcaag ttccgttctc
     1381 tgcatggata gtccactcct ctttatacga tgaatatagc tattgtagca ggtattttat
     1441 aaaaagtaaa gaggggacta tggcaaacgt tgctcttgcc gcctttcctt tatctctcac
     1501 cccaccgcct tcaccagtcc ttccgctgca taccttcccg ctcgcagcgc ttcgtccagc
     1561 gtgttcgcgt ggccgcctat ttctatcaaa aggctgccgg tcgtcaggtc ttggttataa
     1621 aacctgtacc cgcacagcac cggccttgtc agcccgggat acgcaccctc catggccgct
     1681 tcccacgcgg cggcaaatcg caggttcagc cgccagttgg gcagggcggc gctgccgccg
     1741 ttgtcgcacc cggcaatgac catgacctgc gccgtgctct cgccgtcaat ttcacacacc
     1801 ggcttcaccc gcgtgccgtc gctgtcctca atggcatcgc ggtggacgtc cagcacgatt
     1861 ttgatggacg gatatcgcgc cagatagtcc tgcacgcccg cgcggctgcg ctggtagctg
     1921 ctggtgtagc cgggcgcgtc gtagagcgtt gtatcgtgca gggtgtttat gcctgccgcg
     1981 ttcagcaccc gggccatctc ggcccccacg gccaccatgt tcagcgcggt atttttgctg
     2041 cgggccgtgt aattttcgtc aaacaccggg tcgggccagg tctggtagct ctcggtagcg
     2101 tgggtgtgca cgatgagaat ttgcggctca ccgctgtttt tctcgatggc aaacggcagg
     2161 ctctgggtcg tcacggcagc gcgcaggtcg gcgtcggggt gctcggtgct gtttttgatg
     2221 ctgcccgccg ccagctgcac atagccgtcg ccgctgccct gtttgagggt cacgctgcgg
     2281 atgcgcccgg cagattctga tttcggcagc tccggcacgg cgctggacag gggcgtggcc
     2341 gtgggtccgg gcgtcggttc tgcggtggga gcttgtgtcg gggcagggga gggcgtcacc
     2401 gtgggcaacg gcgtcggtcg gcgatcgggc cgctggggtt tgtagacggt ctgggcagcg
     2461 tcggcgggcc agctgacggc agctgccgcg ccggggtcct gcaaaaaaag tgcaccgcgg
     2521 ataagccatg cccatccggg gcaagccgca caggccgccg ccacggccag cacaccgccc
     2581 gcaaacacag ccccgcacag ggccgcagcg gcccaacggc gcagctttcc catggcacac
     2641 acccc
//
LOCUS       DBMDSQ010000276         2639 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_8645_length_2639_cov_2.207644, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000276 DBMDSQ010000000
VERSION     DBMDSQ010000276.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 2639)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2639)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2639
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8645_length_2639_cov_2.207644"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1698
                     /locus_tag="ACZPAX_08855"
     CDS             <1..1698
                     /locus_tag="ACZPAX_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009002687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HIR1114046.1"
                     /translation="GAAVKNIRLGDLLIELGYITPDQLTAALAYQKEHRDLRVGQALQ
                     ELGYVNERQVLEALAKRLQIQLIDIEHTTVDVTAVEKVPQELAQKYDMLPIAQSARTL
                     TVAANDPLNYYAIEDIRQLTGMEPEIVLAELEPLRRAIRYYYAEVRARKAARTANDSD
                     MAAAQTEDFAIDMTAEGGDLDAPIIRLLNSLVQRAVTTTASDIHIEPFEGETKVRMRI
                     DGVIIDYVTLQRALHQPLIARIKIMSNLDIAEHRIPQDGHFRARLETGPDVNVRVSIL
                     PTVFGEKAVLRILSSNTYIKNADHFGMNDATYKRFQPLLNRPNGIVYLTGPTGSGKTT
                     TLYMVLQAIAERQVNISTIEDPVERNLARINQTQVNNIAGLTFESGLRALLRQDPDII
                     MVGETRDAETASISVRAAITGHMVFSTLHTNDALSSIVRLEDMGVERYMIANSVAGLV
                     AQRLMRRVCPHCARQMPVTEEERTYLGPDIPFVRRGTGCTQCNGTGYRGRVAIHELVI
                     INRELREMISAGATQEELTAAARRNQGMTSLREAALQLVREGETTPEELLKITFYEE"
     gene            1702..>2639
                     /locus_tag="ACZPAX_08860"
     CDS             1702..>2639
                     /locus_tag="ACZPAX_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013382398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HIR1114047.1"
                     /translation="MQIHELTALARQMKASDIHISEGLPLMFRIDGRLVNAPVQMSAD
                     ETRSLILGLMDEAHREAVTTERIDADFALVAPDGTRSRVNVFYQQNKAAATLRLLNNS
                     IPTLAELSMPPVLTKLADEPRGLILVTGPTGSGKSTTLAAMIDHINETRSDHIITIEN
                     PIEYVYQGKQALIHQREVGQDVSSFAAALRSALREDPDVILVGEMRDYETISAAVTAA
                     ETGHLVMSTLHTIGAAQTIDRIIDVCPAGAQNQIRGQLAAVLRGVITQQLLPLASGQG
                     RCAATEILVGTDAVANLIREGKYYQIPSVLQSGAALG"
BASE COUNT          528 a          901 c          779 g          431 t
ORIGIN      
        1 ggtgcagccg tgaagaacat ccgactgggc gatctgctga tcgagctggg ctacatcacg
       61 cccgatcagc tgaccgccgc cctggcctac cagaaggagc accgcgacct gcgcgtcggc
      121 caggccctgc aggagctggg ctatgtcaac gaacgccagg tgctggaagc ccttgccaag
      181 cgtttgcaga ttcagctcat cgacattgag cacacgaccg tcgatgtgac cgccgtggaa
      241 aaggtcccgc aggagctggc gcaaaagtac gatatgctcc ccattgcgca gagcgcccgc
      301 accctgaccg tggccgccaa cgacccgctg aactactacg ccatcgagga tatccgccag
      361 ctgaccggca tggagccgga aattgtgctg gccgagctgg agcccctgcg ccgcgccatc
      421 cgctactatt acgccgaggt ccgcgcccgc aaggccgccc gcaccgccaa tgacagcgac
      481 atggccgccg cccagaccga ggatttcgcc atcgacatga ccgccgaggg cggcgatttg
      541 gacgcaccca tcatccgcct tctgaacagc ctggtccagc gcgccgtcac gacgaccgca
      601 tcggatatcc acattgagcc gtttgagggc gaaacaaagg ttcgtatgcg catcgacggc
      661 gtcatcatcg attatgtgac gttacagcgc gcattgcatc agccgctcat cgcgcgtatc
      721 aagatcatgt cgaatcttga cattgccgag caccgcatcc cgcaggacgg ccacttccgc
      781 gcacgtctcg agaccggccc cgacgtcaac gtccgtgtct cgattttgcc gacggtcttt
      841 ggcgaaaagg ccgtgctgcg tattttgtcg tcgaacacct acattaaaaa cgccgaccac
      901 ttcggcatga acgacgcgac ctacaagcgg ttccagcctc tgctcaaccg ccccaacggc
      961 atcgtctacc tgaccgggcc gacaggttcc ggcaaaacga cgacgctgta catggtcttg
     1021 caggccatcg ccgaacggca ggtcaatatc tcgacgattg aggaccccgt ggaacgcaac
     1081 ctggcccgta tcaaccagac gcaggtcaac aacattgcgg gcctgacctt tgagagcggt
     1141 ctgcgcgccc tgctgcgcca ggaccccgac attatcatgg tgggcgagac ccgcgatgcc
     1201 gagacggcgt ccatctcggt ccgcgcggct atcacgggcc acatggtgtt ctcgaccctg
     1261 cacacaaatg acgcactatc gtccattgtc cgtctggagg atatgggcgt ggagcgctac
     1321 atgatagcga actccgtcgc gggcctcgtc gcccagcgtc tgatgcgccg cgtctgcccg
     1381 cactgcgcca ggcagatgcc cgtcaccgag gaggagcgca cctatctcgg ccccgacatc
     1441 ccctttgtgc ggcgcggcac cggctgtacc cagtgcaacg gcacgggcta ccgcggccgc
     1501 gtggcgatcc acgagctggt catcatcaac cgtgagctgc gggagatgat atccgccggg
     1561 gccacgcagg aggaactgac cgccgccgcc cgccgcaacc agggcatgac gagcctgcgc
     1621 gaagccgccc tgcagctggt gcgtgagggt gagaccaccc cggaagaatt gctgaaaatt
     1681 acgttctatg aggaataagt gatgcaaatc cacgagctca ccgcgctggc gcggcagatg
     1741 aaggccagcg atatccatat ttccgagggg cttcccctga tgtttcgcat cgatggccgc
     1801 ctggtcaatg caccggtgca gatgagcgcc gacgagaccc gctcgctgat tctgggcctg
     1861 atggatgagg cccaccgcga ggccgtcacc accgagcgca tcgacgcgga ctttgccctc
     1921 gtcgcgcccg acggcacccg cagccgtgtg aacgtgttct accagcagaa caaggccgcc
     1981 gcgaccctgc gccttttgaa caacagcatc ccgacactgg ccgagctttc gatgcctccg
     2041 gtgctgacga aactggccga cgagccgcgc gggttgatcc tcgtcacagg ccccaccggc
     2101 agcggcaaat ccacgacgct ggccgccatg atcgaccaca tcaacgagac acgcagcgac
     2161 cacatcatca cgatcgagaa ccccatcgag tatgtctatc aaggcaagca ggcgctcatc
     2221 caccagcgcg aagtcgggca ggatgtgtcc agctttgccg ccgccctgcg cagcgctctg
     2281 cgtgaggacc cggacgtcat tctggtcggc gagatgcgcg actatgagac gatctccgcc
     2341 gccgttaccg ccgccgagac cggccacctg gtcatgagca cactgcacac catcggcgcg
     2401 gcgcagacca tcgaccgtat catcgacgtc tgcccggccg gagcacagaa ccagattcgc
     2461 ggtcagctgg ccgccgtgct gcggggcgtt atcacccagc agctgctgcc gctggcaagc
     2521 ggccagggcc gttgcgccgc cacggaaatt cttgtgggca ccgacgccgt ggcgaacctg
     2581 atccgcgagg gcaagtacta ccagatcccg agcgttttgc aatccggcgc ggcgctggg
//
LOCUS       DBMDSQ010000277         2632 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_8668_length_2632_cov_1.511260, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000277 DBMDSQ010000000
VERSION     DBMDSQ010000277.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 2632)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2632)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2632
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8668_length_2632_cov_1.511260"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            36..824
                     /locus_tag="ACZPAX_08865"
     CDS             36..824
                     /locus_tag="ACZPAX_08865"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HIR1114048.1"
                     /translation="MRAFCDDNTPLAFADVTYRFYTVLCDLLHIPQHVIPLESDLTID
                     LKKYCGLHETIVIANPNAPTSLLAPVDAIEEVLKTNPDNIVIVDETYVEFAPAGSSCL
                     PLLETYDNLVITHTFSKTHNLAGARLGFCIARPELIADMNRVKFSYSPYNVNSMTQAA
                     GVAAISDEAYFADVTAKVIAERTHTTDELRRRGFTAFDSATNFLFATTDRMPCKEIFE
                     KLRARGILIRHFNAPRISDYLRITIGTPQQMQRFFAALDEILGV"
     gene            857..1474
                     /gene="hisH"
                     /locus_tag="ACZPAX_08870"
     CDS             857..1474
                     /gene="hisH"
                     /locus_tag="ACZPAX_08870"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005929647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HIR1114049.1"
                     /translation="MIAIVDYGVGNLFSLSSSVKSLGAEVCVTRDADDLRAADHILLP
                     GVGAFADAMAKLEATGLVPVLREETQKKPLLGICLGMQLLFEKSYEYGEHAGLGLIPG
                     AVCPLADDLADPNLKVPHIGWNALDIVPGRENDPLFKYIKNGEYVYYVHSYYAKNCAA
                     STLSTSEYSIPVTGAVRRGLVYGTQFHPEKSGDTGLRLLKAFAEL"
     gene            1490..2209
                     /gene="hisA"
                     /locus_tag="ACZPAX_08875"
     CDS             1490..2209
                     /gene="hisA"
                     /locus_tag="ACZPAX_08875"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HIR1114050.1"
                     /translation="MKLYPAIDLRGGQAVRLYQGDYDQMTVYNADPVAQAQQFIDAGA
                     KYLHVVDLDGAKDDTTANMQTIAAIAKLGGLKIEVGGGIRDEARIHQYLDLGVDRCIL
                     GTVAVKNFAFTQEMAQKYGAQIAVGVDMKDGFVAVNGWKEITPEPGVAFCRRCAEAGV
                     KAIIATDISRDGTMQGTNMALYRELLTIPGLEITASGGIAAMAELAELQAMHCHAAIL
                     GKSIYTGAIDLAKAVRLYEGE"
     gene            2214..>2632
                     /gene="hisF"
                     /locus_tag="ACZPAX_08880"
     CDS             2214..>2632
                     /gene="hisF"
                     /locus_tag="ACZPAX_08880"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HIR1114051.1"
                     /translation="MITKRIIPCLDVKNGRVVKGVNFEGLQDMADPVEMARYYNASGA
                     DELVFYDITASVEGRGLFTDILRQVASQIFIPLTVGGGINTLDDFDRVLKCGADKVSV
                     NSGAIRNPGIIPAAAQKYGNQCVVLSADIKRVDGKFM"
BASE COUNT          559 a          799 c          761 g          513 t
ORIGIN      
        1 agcgatgagc tggatgatta aggccggcac cttcggtgcg ggccttttgc gatgacaata
       61 cgccgctggc ctttgccgat gtgacatacc gcttttacac ggtgctgtgt gatttgctgc
      121 acatcccgca gcacgtcatc ccgctggaaa gtgacctgac catcgacctt aaaaagtact
      181 gcggcctgca tgagacgatc gtcattgcca acccgaacgc cccgacaagc ctgctggccc
      241 cggtggacgc tatcgaggaa gtgctgaaaa cgaaccccga caacatcgtc attgtggacg
      301 agacctacgt cgagtttgcg cccgccgggt cgagctgcct gccgctgctg gaaacctacg
      361 acaacctcgt catcacccac accttctcca aaacccacaa cttggcgggg gcgcggctgg
      421 gcttctgcat cgcgcggccc gagctgattg ccgatatgaa ccgcgtcaag ttcagttaca
      481 gcccctacaa tgtcaattcc atgacccagg cggcgggtgt ggcggccatt tccgacgagg
      541 cctattttgc cgatgtaaca gcaaaggtca ttgccgagcg cacccatacc accgacgagc
      601 tgcgcaggcg cggtttcacc gcgtttgact ctgccacgaa ctttttgttc gccaccaccg
      661 atcgaatgcc ctgcaaggaa atttttgaaa agctccgcgc ccgcggcatc ctcatccgtc
      721 atttcaacgc gccgcgcatc tcggattacc tgcgcatcac catcggcaca ccccagcaga
      781 tgcagcgttt cttcgcagcg ctggatgaga ttttaggggt ttaaggggtc gctcttttta
      841 ggggagggaa tttcacatga ttgctattgt tgactacggc gtgggcaacc tgttcagcct
      901 gtcgtccagt gtcaaaagcc tgggcgcaga ggtctgcgtg acccgcgatg ccgacgattt
      961 gcgggcggcg gaccacattc tgctgcccgg cgtcggggcg tttgccgacg ctatggccaa
     1021 gctcgaggcc accggccttg tgcctgttct gcgcgaagaa acacaaaaaa agccgctgct
     1081 gggcatctgc ctgggtatgc agctgctgtt tgaaaaaagc tatgaatacg gcgagcacgc
     1141 gggcctcggc cttatccccg gcgcagtctg ccccctggca gacgatctgg ccgaccccaa
     1201 tctgaaagta ccacacatcg gctggaacgc gctggacatc gtacccggtc gggaaaacga
     1261 cccgcttttt aagtacatca aaaacggcga gtacgtttac tacgtacaca gctactacgc
     1321 caaaaactgc gctgcatcaa cgctttccac cagcgagtac agcatcccgg tcaccggcgc
     1381 ggtgcggcgg gggctggtgt acggcaccca gttccacccg gaaaaatccg gcgacaccgg
     1441 cctgcggctg ttaaaggcct ttgccgaact gtaaagggga gggaatacca tgaaattata
     1501 ccccgctatt gacctgcgcg gcggccaggc cgtgcggctg tatcagggcg attacgacca
     1561 gatgaccgtc tacaacgccg acccagtggc ccaggcacag caatttatcg acgcaggtgc
     1621 gaagtatctg cacgtcgttg acctggatgg ggccaaggat gacaccaccg cgaatatgca
     1681 gaccatcgcc gccattgcaa aactcggcgg cctgaaaatt gaggtcggcg gcggcatccg
     1741 cgacgaggcg cgcattcatc aatatctcga tttgggcgtt gaccgctgca ttcttggcac
     1801 ggtcgccgtg aaaaattttg cctttaccca agaaatggcg cagaaatacg gcgcacagat
     1861 tgccgtcggt gtggacatga aggacggttt tgtggccgtc aacggctgga aggaaatcac
     1921 ccccgagccg ggcgtggcgt tctgccgccg ctgtgccgaa gcgggcgtca aggccatcat
     1981 cgccacggat atttcccggg atggcacgat gcagggcacc aacatggcgc tgtaccgcga
     2041 acttctgacc atccccgggc tggagatcac cgccagcggc ggcatcgccg ccatggcaga
     2101 actggccgaa ttgcaggcca tgcactgcca cgcggccatc ctgggcaagt ccatctacac
     2161 cggggccatc gatctggcca aggctgtgcg gctgtacgag ggggagtgaa cgcatgatta
     2221 ccaaacgcat tattccttgt ctggacgtca aaaatgggcg cgtcgtcaag ggtgtgaatt
     2281 ttgagggtct gcaggacatg gccgacccgg tcgaaatggc gcggtactat aacgcatccg
     2341 gcgcggacga gctggtcttc tacgatatca ccgccagcgt cgaggggcgc ggactcttca
     2401 ccgatattct gcgccaggtc gccagccaaa ttttcatccc gctgaccgtc ggcggcggca
     2461 tcaacacgct ggacgacttt gaccgcgtgc tgaaatgcgg cgcggacaag gtcagcgtca
     2521 actcgggcgc catccgcaat cccggcatca tcccggctgc ggcgcagaaa tacggcaacc
     2581 agtgcgtggt gctctccgcc gatatcaaac gtgtggacgg caaatttatg tt
//
LOCUS       DBMDSQ010000278         2625 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_8700_length_2625_cov_1.866086, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000278 DBMDSQ010000000
VERSION     DBMDSQ010000278.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 2625)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2625)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2625
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8700_length_2625_cov_1.866086"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1180
                     /locus_tag="ACZPAX_08885"
     CDS             <1..1180
                     /locus_tag="ACZPAX_08885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1114052.1"
                     /translation="FSYNVDRSGLFQGALAPRRIVDLMLQGYDVTNFEQMDERQVVQL
                     YAQDVPETPEAIGIGSSRVLQFNRENTGVESFFNMGVTGADVRDNMTSYYKMVTYGKA
                     PKVLLWSLDPWVFYGSEAAFDARADADLYNEFLTKVLNVPTDYEEPNQVELWKALADP
                     AYFQGNVDYYFKNRGQATVTDDEGNTIEFNPVEGDPYNQPTSIKRSDGSVLYDVAFRS
                     QTEDQIRTLAAEACMSFNSVHMEGFDEMSQTQIQAFDSFIKYAQSQGTTVVLVLSPWH
                     PYLYGYLLTEPEMHKGFFQVENWLRQYCADNNIPLYGSYDPACIEGLQETDFFDGLHC
                     GGTGIAQFFPGIPQALADLQNGTLANPLDVHPRTSLEDPNAQPAEGETPDGETPTQEG
                     "
     gene            1184..1723
                     /locus_tag="ACZPAX_08890"
     CDS             1184..1723
                     /locus_tag="ACZPAX_08890"
                     /inference="COORDINATES: protein motif:HMM:NF036822.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PglD-related sugar-binding protein"
                     /protein_id="HIR1114053.1"
                     /translation="MAFIEIGFNAKRPLVLGRGGFGTQLNDWLMDEGWGSADFLDDNA
                     PGAVGGLRDYVDPAILKRGRPAFVALGDNALRVELLQKLAAAGYATPVFISDAASVSP
                     SAVLEPGCIILPQAYVGADAHLGVGCIVNAGAIVDHNAQLGRGVHVAPGGIVKAGAEV
                     AEFAKVDSGEIIRSPWESK"
     gene            1720..>2625
                     /locus_tag="ACZPAX_08895"
     CDS             1720..>2625
                     /locus_tag="ACZPAX_08895"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HIR1114054.1"
                     /translation="MTQRRDYIDELKGLGILLVVFGHFMEQYRMGYSFVSASFFCIYA
                     FHMALFCTCSGLVARFHPRKLVTQQLWLYLVGQVIMFVFRAAVLRENFAESGGALAAL
                     LLPWRHMWYLYALVFWHLTLPVLCWLRDRWRLPGACLGLALAVGLAFAGGLVEWPFTL
                     VRVVAFYPFYAFGVLFRPQLDALATAAAEKCSLRRAALAALLVGYGAYFLRVLLADPI
                     LDNSPQLFHDVSYADGDTVGYRVVFYLAGILTTAALAVVFSSRHRLEGLGRHTLAIYL
                     LHMPILAALIEFKFYDLMRGKPLAVV"
BASE COUNT          475 a          773 c          841 g          536 t
ORIGIN      
        1 gttcagctat aacgtcgacc gcagcggcct gttccagggt gcgctggccc cccgccgcat
       61 cgtggatttg atgctgcaag ggtacgatgt aacaaacttc gagcagatgg acgagcgcca
      121 ggtggtacag ttgtatgcac aggatgtgcc cgagacgccc gaggctatcg gtattggctc
      181 cagccgcgtt ttgcagttca accgcgagaa caccggcgtg gagagcttct tcaacatggg
      241 cgtcaccggc gcggatgtgc gtgacaatat gacgagctac tacaaaatgg tcacctacgg
      301 caaagcgcca aaggtgctat tgtggagcct ggatccctgg gtcttttacg gcagcgaggc
      361 cgcctttgac gcccgtgctg acgccgattt gtacaatgaa tttttgacaa aagtcctgaa
      421 cgtacccacc gactatgaag agccgaacca ggtcgagctg tggaaagccc ttgccgaccc
      481 ggcatacttc cagggcaacg tggactatta tttcaagaac cgcggccagg ccaccgtcac
      541 cgacgacgaa ggcaacacca tcgagtttaa cccggtggag ggcgacccct acaaccagcc
      601 cacgagcatc aagcggtctg acggcagcgt tttgtacgat gtagcgttcc gcagccagac
      661 tgaagaccag atccgcacgc tggctgccga agcctgcatg agcttcaaca gcgtacatat
      721 ggaaggcttt gacgagatga gccagaccca gattcaggcg tttgactcct tcatcaaata
      781 cgcccagtct cagggcacca cggtcgtttt ggtgctgtcg ccgtggcatc cctatctgta
      841 cggttacctg ctgaccgagc cggagatgca caagggtttc ttccaggtag aaaactggct
      901 gcgccagtac tgcgccgaca acaatattcc gctgtacggc agctatgacc ccgcatgtat
      961 cgagggcttg caggaaacgg atttcttcga cggtctgcac tgcggcggca ccggcattgc
     1021 ccagttcttc cccggtatcc cacaggcatt ggccgatttg cagaacggta cactggcgaa
     1081 cccgctggac gtacaccccc gcaccagtct tgaggacccc aacgcgcaac ccgccgaggg
     1141 cgagaccccc gacggagaga cacctacaca ggagggatga tctatggcat tcatcgagat
     1201 cggttttaac gcaaagcgac cgctggttct gggccgcggc ggctttggca cccagctgaa
     1261 cgactggctg atggacgaag gctggggcag cgcggacttt ttggacgaca acgcccccgg
     1321 cgccgtgggc ggcctgcggg actacgttga ccccgcgatt ttgaaacgcg gccgccccgc
     1381 ctttgtggcg ctgggtgaca acgccctgcg ggtagaactg ctgcaaaagc tggccgctgc
     1441 cgggtacgcg acgccagtct ttatcagtga tgccgcgtcg gtcagcccca gcgccgtgct
     1501 ggagccgggc tgcatcatcc tgccgcaggc ctacgtgggt gcagacgcgc acctgggcgt
     1561 gggctgcatc gtgaacgccg gggccatcgt ggaccacaac gcccaactgg gtcgcggcgt
     1621 acacgtggca ccgggcggca tcgtcaaggc cggggcagag gttgccgagt tcgccaaggt
     1681 ggactccggc gagatcatcc gctccccctg ggagagcaaa tgacccagcg ccgcgattat
     1741 attgacgagc tgaagggcct gggtatcctg ctggtggtgt tcggccattt tatggaacag
     1801 taccgcatgg gatattcctt cgtcagtgcg tcatttttct gtatctatgc gtttcacatg
     1861 gcgctgttct gcacctgcag cgggctggtg gcgcggttcc atccgcgcaa gctggtcacg
     1921 cagcagctat ggctgtatct ggtggggcag gtcatcatgt ttgtgttccg cgcggcggtg
     1981 ctgcgggaaa attttgccga gagcggcggc gcactggccg cgctgctgct gccctggcgg
     2041 catatgtggt acctgtacgc gctggtgttc tggcacctga cgctgccggt tttgtgctgg
     2101 ctgcgggacc gctggcggct gcccggggcc tgcctggggc tggcgcttgc cgtggggctg
     2161 gcgtttgcgg gcggcctggt ggagtggccg ttcacgctgg tacgcgtggt agcgttctat
     2221 ccgttctacg cttttggagt gctgttccgc ccccaactgg atgccctggc taccgcagcg
     2281 gcggaaaagt gcagcctgcg gcgggcggcc ctggccgcgc tgttggtggg gtatggcgcg
     2341 tatttcctgc gcgtgctgct ggctgacccg attttggaca acagcccaca gctgttccat
     2401 gatgtgagtt atgcggacgg cgacacggtg ggctaccgcg ttgtgttcta cctggcgggc
     2461 atcctgacca cagctgcact ggcggtggtg ttcagcagcc gccaccggct ggaagggctg
     2521 gggcggcaca cgctggcgat ttacctttta catatgccga ttctggcggc actgatcgag
     2581 ttcaagttct acgacttaat gcgcggcaag ccgctggcag tggtg
//
LOCUS       DBMDSQ010000279         2619 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_8716_length_2619_cov_1.636616, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000279 DBMDSQ010000000
VERSION     DBMDSQ010000279.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 2619)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2619)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2619
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8716_length_2619_cov_1.636616"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..594)
                     /locus_tag="ACZPAX_08900"
     CDS             complement(<1..594)
                     /locus_tag="ACZPAX_08900"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005926656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HIR1114055.1"
                     /translation="MAYYFSEPSRTFGEYLLVPGYSSSECVPTAVSLKTPLVKYRKGE
                     EACPLEMNIPMVSAIMQSVSGEKLAIALAKQGGVSFIYGSQTIEQEADMVRRVKAYKK
                     GFVTSDSNLPPEATLGDVLDLKTRTGHSTVAITDDGSAHGKLLGIVASRDYRLSRMTM
                     DLKVTEFMTPLDKLVTAPATTSLHDCNDIIWDNKINSL"
     gene            complement(985..2175)
                     /locus_tag="ACZPAX_08905"
     CDS             complement(985..2175)
                     /locus_tag="ACZPAX_08905"
                     /inference="COORDINATES: protein motif:HMM:NF024743.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAS signaling domain-containing protein"
                     /protein_id="HIR1114056.1"
                     /translation="MKKYEYLSELEKRLSALPADARRDALNYYEEYFDAAGTDNEDAT
                     AAELGDPAEAARKILEGEGLTPDDATPEETPLTPPTAPEPPAADPQPAPPRRSPKAKT
                     MWLIFAVVIVAALIIQLGVLVLSFAKPGGGTASMVVAPTEEETAASGLVRTEDFYNGT
                     AATEDADSGSPLEKMDFTTGLADAKNITFDIDYGAVTVVVDSGAAEPTLSCTNLRQDW
                     FNFTNTGDNTSPVRVSYKVPANYNLGKELGPEPEFVLSVPDANTFHFKRLSITAAMGD
                     AEFDNSNTIAADSIDLDLAMGNFTGSTVQAEQFTANISMGDFDLGLLAGVETAKVEAA
                     MGNIGLTVDGRPDDYALDLQTSMGNVVFNGRTMSSIYTQTAAAPRSVTLTASMGDVVL
                     TTTQ"
     gene            complement(2172..2492)
                     /locus_tag="ACZPAX_08910"
     CDS             complement(2172..2492)
                     /locus_tag="ACZPAX_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009260358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HIR1114057.1"
                     /translation="MAINTTAGLLDAIVLAVVGREETGTYGYKITQEVRAVLELSEST
                     LYPVLRRLQKDGCLAVHDEAFNGRNRRYYQITPAGRARLAELTAEWEAYTAKINKLFK
                     GEPQ"
BASE COUNT          494 a          719 c          827 g          579 t
ORIGIN      
        1 cagggagttg atcttgttgt cccagatgat atcattgcag tcgtgcaggc tggtggtggc
       61 gggggccgtg accagcttat ccagcggagt catgaactca gtgaccttga gatccatcgt
      121 catgcgggag aggcggtagt cgcgggaggc gacgatgccc agcagcttgc catgggcaga
      181 gccgtcatcg gtgatggcaa cggtggaatg gccggtgcgg gtcttgaggt ccagcacgtc
      241 gcccaaggta gcctccggcg gcaggttgga gtcggaagtg acaaagccct tcttgtaagc
      301 cttgacacgg cgaaccatgt cggcctcctg ctcgatagtc tgggagccgt agatgaagga
      361 gacgccgccc tgcttggcca gggcaatcgc cagtttctca ccggagacgg actgcatgat
      421 ggcggagacc atcgggatgt tcatttccag cgggcaggct tcctcgccct tgcggtattt
      481 gaccagcggg gttttcaggc tgacagccgt agggacgcac tctgaagagg aatacccggg
      541 aacgagcaga tactcaccaa aggtgcggga cggctcggaa aaatagtatg ccataataca
      601 ctctcctgac tttccttttt tcgacggttt ttacttatta gaaagattgt accacaaccg
      661 cggacaaaat acaaaggatt cggcgaaaat ctttcttgca ccgcgaaaag gaaagttgtg
      721 gattttgaac aatggattgg gtgtgtagta tgactttggg gtggccgggc gggccgatgc
      781 gagcatcggc cccctataag ggcggcagct atggcgaggt ggaagagccc ctcaggcctt
      841 gctgcgcgcg gccagctccc cacttcgtga ggagcctttt tgcgcgggca aagcccgcgt
      901 gaaagtctcc tcgctttgcg gggaggtggt cgagcgtcag cgatgaccgg aggggctctg
      961 gccgtttgct atacgcgggc gggattactg cgtcgtcgtc agcactacat cgcccatcga
     1021 cgcggtaagg gtcacgctgc ggggcgcggc ggccgtttgg gtgtagatac tgctcatcgt
     1081 cctgccgttg aacaccacat tccccatgga cgtttgcagg tcaagcgcgt aatcgtcggg
     1141 cctgccgtcc accgtcaggc caatattgcc catggcagct tcgactttag cggtttcaac
     1201 gcccgccagc agccccaaat caaaatcgcc cattgagata ttcgccgtaa actgctctgc
     1261 ctgcacggtg ctaccggtaa agttgcccat cgccagatct aagtcgatgc tgtcggcggc
     1321 gatggtattg ctgttgtcaa attctgcatc gcccatagcg gcagtgatgg agagcctttt
     1381 aaaatggaag gtattcgcat ccggcacgga caggacaaac tcaggctcgg gccccagctc
     1441 tttgccgagg ttgtagttgg cgggcacctt gtagctgaca cgcaccgggc ttgtgttgtc
     1501 gcccgtgttg gtaaagttga accagtcctg acgcaggttc gtgcaggaaa gggtcggctc
     1561 cgccgcacca ctgtccacaa cgactgtgac cgcgccgtag tcgatgtcga aggttatatt
     1621 tttggcgtcg gccagaccgg tggtgaaatc catcttctcc aatgggctgc cgctgtccgc
     1681 gtcctcggtg gcggcggtcc cgttgtaaaa gtcctcggtg cggacaagac cggatgcagc
     1741 agtttcttcc tccgtcggcg caacaaccat cgacgccgtg ccgccgccgg gctttgcaaa
     1801 actcagcaca aggacgccca gctgaatgat cagcgccgca acaatgacaa cggcaaaaat
     1861 cagccacatt gtttttgctt tggggctgcg gcgcggcggc gcaggctgtg ggtcggcggc
     1921 gggcggttcg ggtgcagtcg gcggggtcag gggcgtttcc tcgggcgtgg catcgtcggg
     1981 cgtcaggccc tccccttcca ggatcttgcg ggcggcctcg gcagggtcgc ccagctcggc
     2041 ggcggtagcg tcctcgttat cggtacctgc ggcgtcgaag tattcctcat aatagttcag
     2101 cgcgtcgcgg cgcgcatcag ctggcagggc agacagccgt ttttccagtt cggataagta
     2161 ctcgtatttt ttcattgcgg ctccccctta aagagcttgt tgattttggc ggtgtaggct
     2221 tcccactcgg cggtaagttc cgccagccgt gcgcggcccg cgggcgtgat ttgatagtag
     2281 cggcggttgc ggccgttgaa ggcttcgtcg tggacggcca gacagccgtc cttttgcagg
     2341 cggcgcagca ccgggtagag cgtggattcc gataattcca gcaccgcgcg gacttcctgg
     2401 gtgattttgt aaccgtaggt gcccgtttcc tcccgcccga cgacggccaa aacgatcgcg
     2461 tccagcagcc ccgccgtggt attgatggcc atagggtgtc ccccttccgg gtgtatggtg
     2521 tatagggtta tatattatat agcatataat attgtagatt gcaataggct gccgcaacaa
     2581 aattatgtag gggcggattc catatccgcc cgtggacgt
//
LOCUS       DBMDSQ010000280         2617 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_8723_length_2617_cov_1.893879, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000280 DBMDSQ010000000
VERSION     DBMDSQ010000280.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 2617)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2617)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2617
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8723_length_2617_cov_1.893879"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1820
                     /locus_tag="ACZPAX_08915"
     CDS             <1..1820
                     /locus_tag="ACZPAX_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HIR1114058.1"
                     /translation="KPGRYTGGEPGCIYKDKSKVDVRMAFCFPDTYEIGMSFLGEKIL
                     YDILNRHENWWCERAFMPWTDMKEQMERLDIPLYCLESKDPLCDFDIVGFSLEYELAY
                     TNVLAMLRLSGIPLRAADRDERWPLIIAGGPCVCNAEPMADFFDIMQLGEGEQMLQDI
                     CATVEQGKKQGWNKEQLLLALAKIPGVYVPSFYDVTYKEDGRIEAVTPNRPGIPARVT
                     KAIVKDMDSFTPPENFVVPLVGAVQDRACVEVLRGCVRGCRFCQAGQLYRPFRERSPK
                     LISDSARALCRNTGYDELSLSSLSTSDHSRLEEILDNLNSWAEADHVSLSLPSLRVDN
                     FSESLVEKTTKVRKSGLTFAAEAGTQRLRDVINKNVTWEQIERGCRIAFSEGYTSVKL
                     YFMMGLPTETLDDIKGIADTAQQVVDLFYKIPNRPKGKGVQVTISVACFVPKPDTPFQ
                     FCAQDRREALREKQKYLLSCVHSRKIKVNYHDSTTSVLEGVFAKGDRRMGRVIETAFE
                     NGAFFDTWEEYFDYDRWLDAFKTCGIDPDFYNYRAIGLDEVTPWDHLDVGVTKAHLVR
                     EYKKALEARTTQPCNRQCSACGANKLIGGPCFDYNQDIL"
     gene            1795..2445
                     /locus_tag="ACZPAX_08920"
     CDS             1795..2445
                     /locus_tag="ACZPAX_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005925644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03936 family radical SAM-associated protein"
                     /protein_id="HIR1114059.1"
                     /translation="MTTIRIFFEKCGEAAYISLLDLQRVFHRMLKMSSLPVYYTQGFN
                     PHIYLSFTCPLSLGQESLCESCEVKTEQETIDPQTWIDALQPLMPRGIVITKIAPAVE
                     KVGEIETAAYEVTMPASSAIALDAYNNAEHAEVTKKTKRGQKTLELKNYLDRIAYTVE
                     GDTVRFTAMLPCGSGEALSLNPSLLTDFLRTQGAAPVWQCRVLRTHLYNKAGKDFE"
BASE COUNT          591 a          773 c          754 g          499 t
ORIGIN      
        1 aaaagccggg ccgttacacg ggcggcgagc cgggctgcat ctacaaagac aaatccaaag
       61 tggatgtgcg catggcgttc tgcttcccgg acacttacga aatcggtatg tcgttcctgg
      121 gcgaaaaaat cctgtacgac attctgaacc gccacgaaaa ctggtggtgt gagcgtgcct
      181 ttatgccctg gaccgatatg aaagaacaga tggagcggct ggacattccg ctgtactgcc
      241 tcgagagcaa ggatccgctg tgcgattttg atatcgtcgg cttctcgctg gaatatgagc
      301 tggcctacac aaacgtcctg gctatgctgc gcctgtcggg tattccgctg cgggcggctg
      361 accgcgacga gcgctggccg ctcattattg cgggcggccc ctgcgtctgc aacgccgagc
      421 cgatggcgga tttcttcgac attatgcagc tgggcgaggg cgaacagatg ctgcaagata
      481 tctgcgcaac ggttgaacag ggcaaaaagc agggctggaa caaagaacag ctgctgctgg
      541 cgctggcgaa gatccccggt gtctacgtgc cgtccttcta cgatgtgacc tacaaggagg
      601 atggccgcat tgaggccgtg accccgaacc gcccgggcat cccggcccgc gtgaccaagg
      661 ccatcgtcaa ggatatggac agtttcaccc cgccggaaaa cttcgttgta ccgcttgtcg
      721 gtgcggtgca ggatcgcgcc tgtgtcgagg tgctgcgcgg ctgcgtgcgc ggctgccgct
      781 tctgccaggc gggccagctc taccggcctt tccgcgagcg cagccctaag ctcatcagcg
      841 acagcgcccg tgcgctctgc cgcaacaccg gctatgacga actgagcctg tcgagcctgt
      901 ccacctccga ccattcccgg ctggaagaaa ttttggataa tttgaactcc tgggccgagg
      961 ctgaccatgt cagcctgtcg ctgccgtcac tgcgcgtgga caatttctcg gaatctctgg
     1021 tcgagaaaac gacgaaagta cgcaaaagcg gcctgacctt tgccgccgaa gccggcaccc
     1081 agcgcctgcg cgacgttatc aacaaaaacg tcacctggga gcagatcgag cgcggctgcc
     1141 gcattgcctt ctcggaaggg tacacctcgg tcaagctcta ctttatgatg ggcctgccca
     1201 ccgaaacact ggacgatatc aagggtattg ccgataccgc ccagcaggtc gtggatctct
     1261 tctacaaaat ccccaaccgc ccgaagggca agggcgtaca ggtgacgatc agcgtggcct
     1321 gctttgtgcc caagcccgac acgccgttcc agttctgcgc ccaggaccgc cgcgaagccc
     1381 tgcgcgagaa gcagaaatat ctgctcagct gcgtgcattc ccgcaaaatc aaggtcaact
     1441 accacgacag caccaccagc gtgctggaag gcgttttcgc caagggcgac cgccgcatgg
     1501 gccgcgttat cgagacagcg ttcgagaacg gcgcgttctt cgatacgtgg gaagaatact
     1561 ttgactatga ccgctggctg gatgccttta agacctgcgg tatcgacccg gacttctaca
     1621 actaccgcgc catcggcctg gacgaggtca caccctggga tcatcttgac gtcggcgtga
     1681 caaaggccca tctggtgcgg gagtacaaaa aagcgctgga agccaggacc acccagccct
     1741 gcaaccgcca gtgcagcgcc tgcggtgcca ataaactgat cggaggaccg tgctttgact
     1801 acaatcagga tattctttga aaagtgcggt gaggccgcgt atatctcgct gctggatttg
     1861 cagcgggtgt tccaccggat gctgaaaatg agcagcctgc cggtgtacta tacccagggt
     1921 ttcaaccccc atatctacct gtccttcacc tgcccgctgt ctctgggaca ggagagctta
     1981 tgtgagagct gcgaggtcaa gaccgaacag gagacaatcg acccgcaaac ctggatcgac
     2041 gctttgcagc ccttgatgcc gcggggcatt gtcatcacaa aaattgcccc ggcggtggaa
     2101 aaagtcggcg agatcgagac agcggcctat gaggtcacga tgccggcaag cagtgccatt
     2161 gcgctggatg cctacaacaa cgccgagcac gccgaggtca cgaaaaagac gaagcgcgga
     2221 caaaaaacgc tggaactgaa gaactactta gaccgcattg cctacacggt ggagggggac
     2281 acggtgcgct ttacggcaat gctgccctgc ggcagcggcg aggcactgag cctgaacccc
     2341 tcgctgctga cggatttcct gcgcacccag ggtgccgccc cggtctggca gtgccgtgtg
     2401 ctgcgcacgc acctttataa taaggcgggc aaagacttcg agtaagaaaa gccttccccc
     2461 gcagaggatg gcaaaagttg aaaaaatctt cgattttctg tcaaaacccc cttgccaaac
     2521 ttcctgcaca atgatataat actaaagcgt tagtgccgcc gagccatcgg cgggataatg
     2581 acacatcatt atacaggcgg tcctctgacg aaatatc
//
LOCUS       DBMDSQ010000281         2617 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_8724_length_2617_cov_1.596184, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000281 DBMDSQ010000000
VERSION     DBMDSQ010000281.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 2617)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2617)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2617
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8724_length_2617_cov_1.596184"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(81..156)
                     /locus_tag="ACZPAX_08925"
     tRNA            complement(81..156)
                     /locus_tag="ACZPAX_08925"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(121..123),aa:Thr,seq:tgt)
     gene            complement(210..1073)
                     /gene="rsmA"
                     /locus_tag="ACZPAX_08930"
     CDS             complement(210..1073)
                     /gene="rsmA"
                     /locus_tag="ACZPAX_08930"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0000154 - rRNA modification [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HIR1114060.1"
                     /translation="MPTLTDLSTIRDLCARYDFALSKRFGQNFIINPGICPRIAEAAG
                     IGPGWGALEVGPGIGVLTEQLCKRADKVVSIEVDKRLPPLLEETMADYDNFKLVLNDV
                     LKVNLKALLAEEFGDKPVAVCANLPYYITSPILMRLLEEKLPIRNITVMVQKEAAQRL
                     CAAPGTRDAGAISYAVAYYAEPKLLFSVQPGSFYPAPKVTSAVIRLDVREKPAVQVPN
                     GDEAAFFALIRAAFSQRRKTAANAIANGLHLPKAQVIAALQAANLDERARPEQLTLED
                     YCALQTAMMQE"
     gene            complement(1105..2322)
                     /locus_tag="ACZPAX_08935"
     CDS             complement(1105..2322)
                     /locus_tag="ACZPAX_08935"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HIR1114061.1"
                     /translation="MSKKISLGVAATIAIIAMAVTFSLTMVVSMKMFNTTVSSVKNKE
                     RQYNKLSEIDRFVRSEEFFTIDEDTLNDRLAAGYMNGINDKYAVYYTAKEYSEKQAVE
                     KGTLTGIGVAVVNDASSGYARIIRLYDNSPAAEAGMQVGGFITAINDESTRNITSSAR
                     LTSKLLGEEGTTTAVTYLTPDRQEQQFNLVHSNYKTPSIYAAQMVADTCGYIRIDSFS
                     AGTASEFKSAVDDLLQQGATAFVFDLRDNTGENLNAALVAADYCVPSGDIAKKQDKDG
                     TVTTLRMSDEDEITVPMVCLVNGSTAGSAELFANALRKMGGATIVGTKTAGKGVVMSD
                     AQSFSDGSAAYITVGLLLDNEDQTWNGEGLSPDIDASLSVDEQNAYYDYTLNTDPQIN
                     KAVNAAMTLTGQN"
BASE COUNT          544 a          692 c          767 g          614 t
ORIGIN      
        1 cgccttattc atgaacagcg tcacaataag gtttgttcta tattgcactg tacattgcat
       61 ctttattttt cacactcctt tggagcgagt aataggactc gaacctacaa cctgctgatt
      121 acaaatcagc tgcactgcca attgtgctat actagcggga acttatttag tttaacacaa
      181 ataagccctg tttgtcaatg gcttaatcgt tattcctgca tcattgctgt ctgcaatgca
      241 caataatctt ccagtgtcag ctgctcaggg cgggcgcgct cgtcgagatt agcagcttgc
      301 aaagcagcta tgacctgggc tttgggcagg tgcaggccgt tggcaatggc gttggcggca
      361 gtcttgcggc gctgggagaa ggccgcacgg atgagggcga agaaggcggc ctcgtcgccg
      421 ttgggtacct gtacggcggg cttctcgcgc acatccaggc ggatgacggc gctggtcact
      481 ttgggggccg ggtaaaagct gccgggctga acgctgaaca gcagtttcgg ctcagcgtaa
      541 taggccacgg cgtagctgat ggcacccgcg tcccgcgtgc caggggcagc acacaggcgc
      601 tgtgcggcct ccttttgcac catgacggta atgttgcgga tgggcagctt ttcttccagc
      661 aggcgcatta aaatcgggct ggtgatgtag tacggcaggt tggcgcagac agccaccggc
      721 ttatcgccaa actcttccgc cagcagagct ttcaagttga ccttcagcac atcgtttaag
      781 acgagcttga agttgtcgta atcagccatc gtctcttcca gcaggggcgg cagacgtttg
      841 tcgacctcaa tggagacgac cttatccgca cgcttgcaga gctgttcggt caatacaccg
      901 atgccggggc cgacttccag cgcaccccag ccggggccga taccggcggc ctcggcgatg
      961 cgagggcaga tgccggggtt gatgataaag ttctggccaa agcgcttgga tagggcaaaa
     1021 tcgtaccgtg cgcacaggtc acggatggta gacaaatcgg tcagtgtggg cacggcggac
     1081 ttcctttccc catggtcaag aggttcaatt ctggccggtc agcgtcatgg ccgcgttgac
     1141 ggccttgttg atctgcgggt cagtgttgag ggtgtaatcg tagtaggcat tctgctcgtc
     1201 gacggacagc gaggcatcaa tatccgggct caggccctcg ccgttccaag tttggtcttc
     1261 attgtccagt agcaggccga ctgtgatata ggcggcagag ccgtcggaaa agctctgggc
     1321 atcgctcatc acaacgccct tgccggcagt cttggtgccg acgatggtcg cgccgcccat
     1381 cttgcgcagg gcgttggcaa acagctctgc actgcccgcc gtgctgccgt tgaccagaca
     1441 gaccatcggg acggtgattt catcttcgtc ggacatacgc agcgtagtca cggtgccgtc
     1501 cttgtcctgc tttttggcaa tatcaccgga gggcacacaa taatcagcgg cgaccagggc
     1561 tgcgttcaag ttctcgccgg tgttgtcacg caggtcaaag acaaacgcag tcgcaccctg
     1621 ctgcagcaaa tcgtccacag cggatttaaa ctcactggct gtgccggcag agaaggaatc
     1681 gatgcggata tagccgcagg tatccgcgac catctgggcg gcgtaaatcg aaggcgtctt
     1741 gtagttagag tgtacgaggt tgaactgctg ctcctggcgg tcgggcgtca ggtaggtgac
     1801 ggcggtggtc gtgccctctt cgcccagcag tttggaggtc aagcgtgcgc tggacgtgat
     1861 attgcgagtg gactcatcat tgatggcggt gataaagccg ccgacctgca taccagcctc
     1921 ggccgcaggt gagttatcat acaggcggat aatgcgggcg tagccggagg aggcgtcatt
     1981 gacgacggcc acgccgatgc cggttagggt gcctttttcg acggcctgct tttcgctgta
     2041 ctccttggcg gtgtagtaga cggcgtactt atcgttgatg ccgttcatat aaccggcagc
     2101 caggcggtcg ttcagcgtat cctcatcgat ggtgaagaat tcttccgaac ggacgaagcg
     2161 gtcgatttcg gagagcttgt tgtattgacg ctccttattt ttgacactgg aaacggtggt
     2221 gttgaacatt ttcatcgata cgaccatcgt cagcgagaag gtgacggcca ttgcgataat
     2281 ggcaatcgtg gcggccacgc caagactgat ttttttgctc atgggtctga tggctcctta
     2341 ctgtttttgc gttttatgcc ccgtaggcaa gcgagaaaat taccgtagag aaaaggctca
     2401 gcaccatcag aacggcgacc accaggcaaa aaatgcgcac ggaggtctta tgcttctgcg
     2461 gtttcatagc aaactcccct ttcgtttaag agagataatt catgacattg accaggacgt
     2521 tattgacctt catctccaag tgcaggtggt tgcccgtgga attgccgcct tcccttcata
     2581 tcccaaacct tcacaaggga cgaaatcccc ctttgca
//
LOCUS       DBMDSQ010000282         2601 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_8792_length_2601_cov_1.468000, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000282 DBMDSQ010000000
VERSION     DBMDSQ010000282.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 2601)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2601)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2601
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8792_length_2601_cov_1.468000"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            126..1082
                     /locus_tag="ACZPAX_08940"
     CDS             126..1082
                     /locus_tag="ACZPAX_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1114062.1"
                     /translation="MVNHLKKYFPIKGGLLSQTVGYVKAVDGVTFNLRRGCTMGLVGE
                     SGCGKSTTGRTILRLGGEKTGGQVLFNGKEVYDMTPAEMRALRTQMQIIFQDPFSSLS
                     PRLPVGEIIGEAVREHNLVPKNEFNDYIDQVMDDCGLQPYHKTRYPHEFSGGQRQRIC
                     IARALALNPEFVVCDEPVSALDVSIQAQIINLLKSLQEKRNLTYLFISHDLSVVEHIS
                     DTVGVMYLGNLVEYGETDDIFAHPLHPYTKALFSAIPIPDPTVKRERIVLQGSIPSPA
                     NPPSGCKFHTRCPYATERCKTEAPKQREAEPGHYVVCHLYDK"
     gene            1079..1354
                     /locus_tag="ACZPAX_08945"
     CDS             1079..1354
                     /locus_tag="ACZPAX_08945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1114063.1"
                     /translation="MRKHDDFEDDGRTIADMSDIGPAPMLRPHRRKAGTPPPEPANDR
                     PWEQAPDWTAKEKFWAVLGAMKATLLIAGVYLAGLAVLLLILFLLWK"
     gene            complement(1431..>2601)
                     /locus_tag="ACZPAX_08950"
     CDS             complement(1431..>2601)
                     /locus_tag="ACZPAX_08950"
                     /inference="COORDINATES: protein motif:HMM:NF020939.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HIR1114064.1"
                     /translation="RDLQRLQTMERYCKTTACLRNYILEYFGEHPAAPCGNCGSCNNV
                     YEQADLTAAAKWMINCVAETRGRFGKAIVFGTLQGANRARLKEIGATNYKSYGQLKDL
                     PRDILDRLLAQLLEDGYLVQTDDKYAVLRMGDITPLKQPGAQVLVKLPPRQEPERVQR
                     PKKRSVMDALTKAGLELYQQLRTLRFEIAQREGLPPYVVFGDKSLANMCLRAPATVEG
                     MIGIYGMGERKYEKYADQFFAVIDAYRADHPDAVLSLDAPDSEPAPETPAKTAKPGSK
                     ATKAAFCLTEERAAAFAYQDALSPAEMKEALAAVAAPDMKAPTIKDMEGWLLDERLIA
                     MEKLPTKGFYYVPTPAGVDAGLRSEDRVSARGTPYSVLQFTPPAQKLIVEHYIKK"
BASE COUNT          491 a          828 c          768 g          514 t
ORIGIN      
        1 aggtcagctg ctaccgctac tatgaagaaa acagaaaggc gggtgagtga cgcatggcaa
       61 agaaggaaaa acttctggtt gaaaacgact acacccccgt cacctacgac cctcaatata
      121 ttctgatggt caaccacctc aaaaagtatt tccccatcaa gggcggcctg ctgtcccaga
      181 ctgtgggcta tgttaaggct gtggacggcg ttaccttcaa cctgcgccgc ggctgcacga
      241 tgggcctggt cggtgagtcc ggatgcggaa aatccacgac tggccgtacc attctgcgcc
      301 tgggcggtga aaagaccggc ggccaggtgc tcttcaacgg caaggaagtc tacgacatga
      361 ccccggccga aatgcgtgcg ctgcgtaccc aaatgcagat catcttccag gatccgttct
      421 ccagcctgtc cccgcgtctg ccggtcggtg aaattatcgg cgaagctgtg cgcgagcaca
      481 acctggtacc gaaaaacgaa tttaacgatt atatcgacca ggtcatggac gactgcggtc
      541 tgcagcccta ccacaagacc cgctacccgc acgaattttc cggcggccag cgccagcgta
      601 tctgcattgc ccgcgccctg gcgctgaacc ctgagttcgt cgtctgcgac gagccggtct
      661 ccgcgctgga cgtttcgatt caggcgcaga tcatcaacct gctcaagagc ctgcaggaaa
      721 agcgcaacct gacctatctc tttatctccc acgacctgtc cgtcgtcgag catatctcgg
      781 acacggtggg cgttatgtac ctgggcaacc tggtggagta cggcgagacc gacgatatct
      841 tcgcccaccc gctgcacccc tacaccaagg cactgttctc ggccatcccc atcccggacc
      901 cgacggtcaa acgcgagcgt atcgtgctgc aaggctcgat cccgtccccg gccaacccgc
      961 cgtccggctg caagttccac acccgctgcc cttacgccac cgagcgctgc aagaccgaag
     1021 caccgaagca gcgtgaggcc gagccgggcc attacgtggt ctgccacctg tacgacaaat
     1081 gaggaagcac gacgattttg aggacgatgg ccgcaccatc gccgatatga gtgatatcgg
     1141 ccctgcgccg atgctgcgcc cccaccgccg caaggcgggc accccgcccc cagaaccggc
     1201 caacgaccgc ccatgggagc aagcccctga ctggaccgcc aaggaaaagt tctgggccgt
     1261 gctgggtgcc atgaaagcaa ccctgctgat tgccggggtc tacctggcgg ggctggcagt
     1321 gctgctgctc atcctgttcc tgctgtggaa ataatggcat aaaactccaa aaggcacaca
     1381 ccgattttgc gtcggtgtgt gcctttttcg caatttacgc gatgtacagc ttattttttg
     1441 atgtaatgtt ccacgattag tttctgcgcc gggggtgtga actgcaaaac cgagtagggc
     1501 gtgccgcggg cgctgacgcg gtcctcgctg cgcaggccgg catctacacc ggcgggggtg
     1561 ggcacgtaat aaaagccctt ggtggggagt ttttccatgg caatcaaccg ctcgtccagc
     1621 agccagccct ccatatcctt gatggtgggg gctttcatgt cgggcgcggc gacggcggcc
     1681 agcgcctcct tcatttcggc aggggagagg gcgtcctggt aggcaaacgc cgcggcgcgt
     1741 tcctcggtca gacagaatgc ggccttggtg gccttgctgc cgggcttggc ggtttttgcg
     1801 ggcgtttccg gtgcgggctc cgaatcgggg gcatccaggc tcagcaccgc gtcggggtgg
     1861 tcggcgcggt aggcgtcgat gaccgcaaag aactggtcgg catacttctc gtacttgcgc
     1921 tcgcccatgc cgtaaatacc gatcatccct tccaccgttg cgggggcacg caggcacata
     1981 ttggccagcg atttgtcgcc gaacacgacg tagggcggca gcccctcgcg ctgggcgatc
     2041 tcgaaccgca gggtgcgcag ctgttggtac agctccagcc cggccttggt cagggcgtcc
     2101 atcacgctgc gctttttggg gcgctgcacc cgctccggct cctgccgggg cggcagcttg
     2161 accagcactt gcgccccggg ctgtttcagc ggcgtgatat cgcccattcg cagcacggcg
     2221 tacttgtcgt cggtctgcac caaatatcca tcttccaaca gctgggccag cagccggtcc
     2281 agtatgtccc tgggcaggtc tttcagctgg ccgtagctct tgtaattcgt cgcgccgatc
     2341 tctttcagcc gggcgcggtt agccccctgc aaggtgccaa agacgatggc cttgccaaac
     2401 cggccccgcg tctcggccac gcagttgatc atccacttgg ccgcggcggt caggtcggcc
     2461 tgttcataca cattgttgca gctgccgcag ttgccgcagg gcgcggcggg gtgctcgccg
     2521 aaatattcca aaatatagtt gcgcaggcag gccgttgtct tgcagtaacg ctccatcgtc
     2581 tgcaaccgct gcaagtcgcg c
//
LOCUS       DBMDSQ010000283         2581 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_8881_length_2581_cov_1.752419, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000283 DBMDSQ010000000
VERSION     DBMDSQ010000283.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 2581)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2581)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2581
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8881_length_2581_cov_1.752419"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            88..927
                     /locus_tag="ACZPAX_08955"
     CDS             88..927
                     /locus_tag="ACZPAX_08955"
                     /inference="COORDINATES: protein motif:HMM:NF018914.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HIR1114065.1"
                     /translation="MKWAGGDTIRAALYILQTGDTDPRTMARTLGLPSIEAAKRALQY
                     WAGAGLLENERGGSPAPAAEPRHADLASMANDPYVAVLSQESQTIFGKGISRNELQRL
                     VDLYQIDGWKPDVILLCCAEVARLGRRTVGAVAHLLSRWREDGVETGEDAERWLKRAK
                     QREDWCSDAAAQFGIAPRDLTNWERRAIARWHEEMGVGHEMIDEALLHANGKNTVRYV
                     DGILRAWRAQGITTVEAARKQGQLEGSNIVMTERPGTQQPAASAQKDLFNRNWAAMFD
                     EEG"
     gene            931..1890
                     /locus_tag="ACZPAX_08960"
     CDS             931..1890
                     /locus_tag="ACZPAX_08960"
                     /inference="COORDINATES: protein motif:HMM:NF013828.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR1114066.1"
                     /translation="MRTKDELFRAAQREVAGYRQQAVMQAETTRRAAYAAHPALAEAD
                     AAHIRAGLGLAKAAALGSSMDAARAALARADETLAAAVKEAGYDESAFKPAYRCPLCE
                     DTGMRGGIPCRCVADVARRLRREEINAASPLGLCQFSSFEVERYGTNVEPELGISPRE
                     YMGKLLNYCKNYAARFSANSPNLLFMGHSGLGKTHLALAIADAVLEGGHDVLYTSAAA
                     LAAQLGKEHFDYTNNEEWLAACQEADLLILDDLGTEYITPLTISVLYELINTRMLTHR
                     PTIYTTNIIDQNIFVARYTEKVASRMLGGCKMFKFFGEDQRLK"
     gene            complement(2041..>2581)
                     /locus_tag="ACZPAX_08965"
     CDS             complement(2041..>2581)
                     /locus_tag="ACZPAX_08965"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HIR1114067.1"
                     /translation="PLGGSRCSTAKKDRNLILTFLVSGLWHGAGLTYLVWGGLHGVCQ
                     AVENHLPHRRSGKLRHALRVLGTFCILAGLFMIFRASSLGNAADVFKGILLNGGHAVF
                     SNYWELGLTSRLEQLMLFAGIAILLGVDAAHERGVSLRGKIAALPTPARWAVYEVCIF
                     AFLFMGRFLGGGSFLYARY"
BASE COUNT          536 a          795 c          826 g          424 t
ORIGIN      
        1 cggaacaaga ggctcgtgac attgccaaca agcgtgaaca gttgcagcgt gagcagggct
       61 tgcgtactca gaaactgctg acattggatg aagtgggccg gcggcgacac gatccgcgcg
      121 gcgctgtaca ttttgcagac cggcgacacc gacccccgca cgatggctcg gacgctgggg
      181 cttcccagca tagaagccgc caagcgtgcc ctgcaatact gggctggtgc cggattgctg
      241 gaaaacgagc gcggcggctc ccctgccccg gcggcagagc cgcgtcacgc cgatttggcc
      301 agcatggcaa acgaccccta tgtggcggtg ctcagccagg aatcccagac catctttggc
      361 aagggcatca gccgcaatga gttacagcgg ctggtggacc tgtaccagat cgacggctgg
      421 aaacccgacg taattttgct ctgctgcgcc gaggtcgccc gcctgggacg gcgcaccgtg
      481 ggcgcggtag cccacctgct gtcccgctgg cgggaggacg gcgttgagac cggagaggac
      541 gccgagcgtt ggctgaaacg cgcaaaacag cgggaggact ggtgcagtga tgccgccgcc
      601 cagtttggca ttgcgccgcg cgatttgacg aactgggagc gccgcgccat cgcccgctgg
      661 cacgaggaaa tgggcgttgg gcatgagatg attgacgagg cgttgctgca tgccaacgga
      721 aaaaacaccg tgcggtatgt ggacggcatc ctgcgggcct ggcgtgccca gggcatcacc
      781 accgtggagg ctgcccgcaa gcagggccag ctggaaggca gcaacattgt gatgaccgag
      841 cgccccggca cccagcagcc ggccgccagc gcccagaaag acctgttcaa ccgcaactgg
      901 gccgctatgt ttgacgagga gggctaaccc atgcgcacaa aagatgaact attccgcgcc
      961 gcccagcggg aggttgccgg gtaccgccag caggctgtaa tgcaggccga gacgacccgc
     1021 cgcgccgcct acgccgcgca ccccgccctg gccgaagcgg acgctgccca catacgggcg
     1081 ggcctaggcc tggcaaaggc cgccgcactg ggcagtagca tggacgccgc ccgtgccgcg
     1141 ctggcccgcg ccgatgaaac gctggccgcc gctgtgaaag aggccgggta cgacgagagc
     1201 gcctttaaac ccgcttaccg ctgccccctg tgcgaggaca ccgggatgcg gggcggcatc
     1261 ccctgccgct gtgtggccga tgtggcccgc cgcctgcgcc gggaggagat caacgccgcc
     1321 agtccgctgg ggctatgcca gttttccagc tttgaggtgg agcggtacgg caccaatgtg
     1381 gagccggagc tgggcatttc cccgcgggaa tacatgggca agctgctgaa ttactgtaag
     1441 aactacgcgg cccgattcag cgccaacagc ccgaatctgc tgttcatggg ccacagcggc
     1501 ctgggcaaga cacatctggc gctggcgatt gcagatgccg tgctggaagg cgggcatgat
     1561 gtactgtaca ccagtgctgc cgcgctggcc gcccagctgg gcaaggagca ctttgactac
     1621 accaacaatg aggaatggct ggccgcctgc caggaggctg acctgctgat tctggacgac
     1681 ctgggcaccg agtacatcac gccgctgacc atcagtgtgc tgtatgagct gatcaacacc
     1741 cggatgctga cccaccgccc aacaatctat accacaaata ttattgacca gaatattttt
     1801 gtagcccgct acaccgagaa agttgccagc cgtatgctgg gcggctgcaa gatgttcaag
     1861 ttctttgggg aagatcagag attaaaataa aaatgggata ggaaccgcac aggttcttat
     1921 cccacttttt tattaccgct attgtagagg cctggtatgc tcggcctgcg cgtacaaggc
     1981 agatgcgcaa ttatggggcg aggctgcggg ccgcacatat gcaacccctc aaccatcagg
     2041 tcaatagcgt gcgtagagga agctgccgcc gccaaggaac cgccccataa agaggaatgc
     2101 gaagatgcag acctcgtata cagcccagcg ggccggggtg ggcagggcgg caatttttcc
     2161 gcgcaggctg acgccgcgct cgtgggcggc atccacgccc agcagaatgg caatgcctgc
     2221 aaagagcata agctgctcca gcctgctggt caggcccagc tcccaataat tgctgaacac
     2281 cgcatggccg ccattgagta ggatgccttt gaatacatct gctgcattgc ccaggctgct
     2341 ggcccggaaa atcataaaca gtccagccag aatgcagaac gtgcccagca cccgcagtgc
     2401 gtgacgcagt ttgccgctgc ggcggtgcgg caggtggttt tccaccgcct ggcagacgcc
     2461 atgcaggccg ccccagacga ggtacgtcag ccctgcgccg tgccacaagc cgctgacaag
     2521 gaaggtcaga atcaggttgc ggtccttttt tgcggtgctg caccggctgc cgcccagcgg
     2581 g
//
LOCUS       DBMDSQ010000284         2577 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_8898_length_2577_cov_1.414782, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000284 DBMDSQ010000000
VERSION     DBMDSQ010000284.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 2577)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2577)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2577
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8898_length_2577_cov_1.414782"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(232..1395)
                     /locus_tag="ACZPAX_08970"
     CDS             complement(232..1395)
                     /locus_tag="ACZPAX_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009324108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR1114068.1"
                     /translation="MPKQNRGPYILAAGAAAQLLTGIPAAWGVFQQPVMQGYGFSRGQ
                     AMLGFAVLVAAYGVGCAVGGLLQDARGPRFAGLWGTALLAGGFFAAAFVPPANAALFL
                     VVYSLPAGLGSAFLAPAVLACAQKWYQDKKGWATGVAGVAMGLSGAFFTVFVKGVGGA
                     WGIRVCFAALGVVMLVVCGAGALILQDPPPQPQRGKPQPGPDFDYKQMLRTPQYKLCV
                     AAVALSAPAVLLFSPEIFKIATERGLPESAAPYSIVLGSAASAAGRMLLPALSDKLGR
                     KPVLYAVYLGLAAGSAWFAFAGQWWVLAAYAVLTFFYSGGAAVQPSFNTDLFGLPHAG
                     VNYGFIALGMSGGSILSYIGAQALPLAARHWLAGGCAAAGLVCVSLVKPVENG"
     gene            1639..>2577
                     /locus_tag="ACZPAX_08975"
     CDS             1639..>2577
                     /locus_tag="ACZPAX_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005922099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HIR1114069.1"
                     /translation="MKTQPLKGMRDLLPREQALRDYIQGQILATYRAAGFQRISTPIL
                     EDMENLDKSDGGDNLNLIFKVLKRGDKLEHALASGNEKQLSDMGLRYDLTLPLSRYYA
                     ANRNELPNPFKVIQTDRVYRAERPQKGRLREFVQCDIDILGDSSPNAEVELIDVTARA
                     LLKIGFTGFTVNINDRRILRAMLEKMGFAEDQLDSVCISFDKMDKIGADGVKAELTEK
                     GFAPEAVQALDDFLRAGDFSLDTVAAKVDDEALTADLRYVIAASEKVANGRYGIAYAP
                     SLVRGQGYYTGMVFEVTCPQFSGAVAGGGRYDNMGGK"
BASE COUNT          551 a          867 c          757 g          402 t
ORIGIN      
        1 aaaaatgccg tggcctactg tgcagccagg cggcgaagct gttgggtttg ggtcatagtg
       61 aaaacctctt aaagcacgta atgtattgcc ggagctgcta ggtaggggcc gcacatgtgc
      121 ggcccgcaac gcaacggaaa tcgaccgtct gcgggaaggc cgcgggccgg gcatgcccgg
      181 cccctacaag ttcccggcaa atccatattg taccctgccg cgcataaaaa ttcaaccatt
      241 ctccacaggt tttacaagac tgacacaaac cagccccgcc gcagcacatc ccccggccag
      301 ccaatgacgg gcggccaggg gcagggcctg cgccccgata tagctcaaaa tactgccgcc
      361 gctcatgccc aaggcgatga atccatagtt taccccggcg tggggcaagc caaacagatc
      421 cgtgttgaag gacggctgca cggccgcgcc gccggagtag aaaaacgtaa gcaccgcata
      481 ggccgccaac acccaccact gccccgcaaa ggcaaaccat gcgctgcccg ccgcaagacc
      541 caggtacacg gcatacaaaa ccggcttgcg gcccagcttg tcactgaggg cgggcagcag
      601 catccgcccc gccgcactgg ccgccgaccc cagcacgata ctgtacggcg cggcgctctc
      661 gggcaggccg cgctcggtgg caattttgaa aatttccggg ctgaacagca gcaccgccgg
      721 ggcggacagc gctaccgccg ccacacacag cttgtactgc ggcgtgcgga gcatctgctt
      781 gtagtcaaaa tccggccccg gctggggctt gccgcgctgg ggttgaggcg gcgggtcttg
      841 caaaatcagc gcccccgcgc cgcagacgac gagcatgacc acccccagcg ccgcaaagca
      901 aacgcggatg ccccacgccc cgccgacgcc cttgacaaac accgtaaaaa acgcgccgga
      961 cagccccatg gccacacccg ccacgccggt ggcccagcct tttttgtcct gataccattt
     1021 ttgggcgcag gccagcaccg ccggggccag aaacgcactg cccagcccgg cgggcaaact
     1081 gtacacgaca agaaacaatg cggcgttggc gggcggcacg aacgccgccg cgaagaaccc
     1141 gcccgccaat agggccgtgc cccacagccc ggcgaaacgc ggaccgcggg catcctgcag
     1201 caagccgccc accgcgcagc ccacaccata ggccgcgacc agcaccgcaa accccagcat
     1261 agcctgcccg cgggaaaagc cgtacccctg catgaccggc tgctggaaca cgccccacgc
     1321 cgccgggatg ccggtgagca gctgtgccgc cgcccccgcc gctaaaatat acggcccgcg
     1381 attttgtttt ggcacacgca tcccctcccc ttttcgggat agtatgcccg gtttggattg
     1441 acaaaatgcg gtttgggcga tatgattata tataaaaagc cttcccctgt gggggaaggt
     1501 ggccgaaggc cggatggggg caggcttgct tctattgccc atttgtaggt cactatggca
     1561 aacgcgcccc tcatcagtca ccttcggtga cagcttcccc ccaagggggg aagccaattt
     1621 gcaataagga gaaccaccat gaagacacaa cctttaaaag gtatgcgcga tctgctcccg
     1681 cgggaacagg cgctgcgtga ttatatccag ggccagatcc tggccaccta ccgcgccgca
     1741 ggcttccagc gcatctccac cccgattttg gaggatatgg agaacctgga caagagcgac
     1801 ggcggcgata atctgaacct gattttcaag gttctcaagc gcggcgacaa gctggagcac
     1861 gcgctggcaa gcggtaacga gaagcagctg tccgacatgg gtctgcgcta tgacctgacg
     1921 ttgccgctgt cccgctacta cgcggccaac cgcaacgagc tgcccaatcc cttcaaggtc
     1981 atccagactg accgcgtcta ccgcgccgag cgcccgcaga agggccgcct gcgcgaattc
     2041 gtccagtgcg acattgacat tctgggcgat tcttccccca acgccgaggt cgagctgatc
     2101 gacgtgacgg cccgcgccct gctgaagatc ggctttaccg ggttcaccgt caacatcaat
     2161 gaccgccgca tcctgcgcgc catgctggaa aagatgggct ttgccgagga ccagctggat
     2221 tccgtctgca tcagctttga caagatggac aaaatcggcg cggacggcgt caaagccgag
     2281 ctgaccgaga agggctttgc ccccgaggcc gtgcaggcgc tggacgactt cctgcgtgca
     2341 ggggatttca gcctggacac cgtcgccgcc aaggtggacg acgaggcact gaccgccgac
     2401 ctgcgttatg tcattgccgc cagcgagaag gtcgcaaacg gccgctacgg cattgcttac
     2461 gcccccagcc tggtgcgcgg ccagggctac tacaccggta tggtgtttga ggtcacctgc
     2521 ccgcagttca gcggtgccgt ggccggcggc ggccgctatg acaacatggg cggcaag
//
LOCUS       DBMDSQ010000285         2575 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_8906_length_2575_cov_1.837510, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000285 DBMDSQ010000000
VERSION     DBMDSQ010000285.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 2575)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2575)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2575
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8906_length_2575_cov_1.837510"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1247)
                     /locus_tag="ACZPAX_08980"
     CDS             complement(<1..1247)
                     /locus_tag="ACZPAX_08980"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HIR1114070.1"
                     /translation="MVFNSVVFLFCFLPLSLLLYYLVPGRAKNAVLLAESLIFYCWTG
                     IEFLPLIAALIVFNYLWALLTAKTGAKLRGLLPAVAVLVNLGVLVYFKYANFLIDTLN
                     QIGHFGIAALNIGAVLPLGISYYIFKIISYEVDVYTGKIKPEKNFLTFAVYVLFFPQL
                     IVGPIVKYREMADQLHDGQNRCTLAKAQRGAELFVFGLAKKVILADSIGALWTDIIGA
                     GGVGLANVSTPLAWLGIIAYSLQLYFDFAGYSEMSNGLAALLGFDCPANFNLPYISGS
                     ITEFWRRWHITLSGWFRDYIYIPLGGNRKGAARQLFNMFLVWAATGIWHGASWNFIAW
                     GLYYFVLLTIEKNFLMKYLKRGKVWPHIYTLFFVVLGWGIFTANQPGAPLGLLLQKLF
                     VPQGGIAPLYYLRNYGVLLALGCV"
     gene            complement(1324..1920)
                     /locus_tag="ACZPAX_08985"
     CDS             complement(1324..1920)
                     /locus_tag="ACZPAX_08985"
                     /inference="COORDINATES: protein motif:HMM:NF025628.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4358 domain-containing protein"
                     /protein_id="HIR1114071.1"
                     /translation="MKKLIPVILCCAMLLAACGSSADSTSTAVSSESSSAAESTIGGA
                     DGPTDIVISESTDASTGEEANTANLDAAVAAIEAVNPIANPRALDDFSVENELMLTMD
                     NLVGYKGDVTNDQADCGLVFVAQAKDGQVDAVKAELEAYKESLSANDLYAEFADKIAL
                     AKDARIVTNGNYVAIVIAGIANPEYAPIDTALETALNF"
     gene            2213..>2575
                     /locus_tag="ACZPAX_08990"
     CDS             2213..>2575
                     /locus_tag="ACZPAX_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005924012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HIR1114072.1"
                     /translation="MRNPKARETAWTGMLFALAIALSWLESMVSPLLGLMPAIKLGLS
                     NIVVMYALLFLRTRTAVLLVVLKALFAFLTRGVTAGFLSLCGGALALAVYLLLLHLPG
                     VSGYIFCTCGALAHNLGQL"
BASE COUNT          588 a          716 c          716 g          555 t
ORIGIN      
        1 acgcagccca gcgccagcag cacaccatag ttgcgcaggt agtacagcgg ggcaatgccg
       61 ccctggggca caaacagctt ttgcagcagc aagcccagcg gtgcgccggg ctggttggcc
      121 gtaaagatgc cccagcccag taccacaaag aacagcgtgt agatgtgcgg ccagactttg
      181 ccccttttca ggtatttcat caaaaagttc ttttcaatgg tcagcagcac aaagtagtaa
      241 agtccccagg cgataaagtt ccagcttgcg ccatgccaga tgccggtagc cgcccagacc
      301 aggaacatat taaaaagctg ccgcgccgcg cctttacggt tgccgcccaa gggaatgtag
      361 atgtaatccc ggaaccagcc cgaaagcgtg atatgccagc gccgccaaaa ttcggtgatg
      421 ctaccggaga tatacggcaa attgaagttg gccgggcaat caaagccaag cagagcagcc
      481 aggccgttgc tcatctcact gtaaccggcg aagtcgaaat acagctgcaa gctgtacgcg
      541 ataatgccca gccatgccag cggggtcgag acattcgcca gccccacgcc acccgcaccg
      601 atgatatcag tccacagcgc accaatggaa tccgccagaa tgactttttt tgcgaggccg
      661 aacacgaaca gttccgcacc tcgctgggcc tttgccagcg tgcagcggtt ttggccatcg
      721 tgcagctggt cggccatctc gcgatatttc acgataggcc cgacaataag ctgcgggaag
      781 aagaggacat ataccgcaaa cgtcagaaaa tttttctccg gtttgatttt gcccgtgtag
      841 acatcgacct catagctgat gattttaaag atgtaatagc tgatgcccag cggcagcacc
      901 gcgccgatat tcagggctgc aatgccaaaa tgtccgattt gattcagcgt gtcgatcaga
      961 aagttggcat acttgaaata caccaatact cccaggttga ccagcaccgc cacggctggc
     1021 agcagaccgc gcaacttagc cccggtcttg gctgtcagca gcgcccacag gtagttgaac
     1081 acaataagcg ctgcaatcag cggcagaaat tcaatgccgg tccagcagta gaaaatcagg
     1141 ctttctgcca gcagcactgc atttttggcg cggccgggca ccagataata cagcagcagg
     1201 gaaagcggca gaaagcagaa caggaacacc acactgttaa aaaccatggc gtactccctt
     1261 cttttgaatc atgttaaaaa agggccgttg ccgaacggca gcggccctgc atcgggtcat
     1321 cttttagaag ttgagggccg tctccagcgc ggtgtcgatg ggggcgtatt ccgggttagc
     1381 gataccggct atgacgatgg cgacatagtt gccgttggtc acaatgcggg cgtccttcgc
     1441 cagggcaatc ttgtcagcaa actcggcgta caggtcgtta gcggacaggc tctctttgta
     1501 ggcttccaac tcggccttca cagcgtcaac ctggccgtcc ttggcctggg ccacaaagac
     1561 aaggccgcaa tctgcctggt cgttggtgac gtcgcccttg tagccgacga ggttgtccat
     1621 cgtcagcatc agctcattct ccacagagaa gtcatccagc gcacgagggt tggcgatggg
     1681 gttgacagcc tcgatagcgg caacggcggc atcaaggttg gcggtgttgg cctcctcgcc
     1741 ggtggaggca tcggtgcttt cgctgatgac gatatccgtc gggccatcgg caccgccgat
     1801 ggtgctttcc gcagcagagc tgctctcaga ggagacagca gtggaggtgc tgtctgcaga
     1861 ggagccgcag gcggccagca gcatagcgca gcaaagaatt acaggaatca gctttttcat
     1921 aggtgaaatt ctccttttat atttcggtaa ggttttccct cacgtatagg cacagttttc
     1981 ccatactttt ataaggatac actatttggc tgtttttttc aagatgtttt gggcgaaaca
     2041 cgagccgccc ggcagcaaaa atatgtaaac tttttatgcc ttgacaaacc gcccttcggc
     2101 agggtatatt caagacaaag taaaaaccag atttgtaagg gcggatgctc gcctccgccc
     2161 gcgggtcgat ggtgagcatc gacccctaca ctatgaggaa gtgaactgcc agatgcgcaa
     2221 tccaaaagcc cgcgagaccg catggaccgg gatgctgttt gcccttgcca tcgcactgtc
     2281 ctggctggaa tccatggtat cgcccctgct ggggctgatg cctgccatta agttagggct
     2341 gtccaatatt gtagtcatgt acgcgctgct gttcctgcgc acccgcacgg cggtattgct
     2401 ggtggtgctc aaggcactgt ttgcctttct gacgcgcggc gtgaccgctg gttttctttc
     2461 cttatgcggc ggggcgctgg cgctggccgt gtatcttttg ctgttgcatc tgcccggggt
     2521 cagcgggtac atattctgca cctgtggggc gctggcgcac aacctcggcc agctg
//
LOCUS       DBMDSQ010000286         2568 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_8937_length_2568_cov_1.635184, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000286 DBMDSQ010000000
VERSION     DBMDSQ010000286.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 2568)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2568)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2568
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8937_length_2568_cov_1.635184"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..956
                     /locus_tag="ACZPAX_08995"
     CDS             <1..956
                     /locus_tag="ACZPAX_08995"
                     /inference="COORDINATES: protein motif:HMM:NF013126.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HIR1114073.1"
                     /translation="TIDDPRADGVTQVTDAMRETLQQYPVGGICQFGKNIESPSQLAR
                     FNKDLQAASATPLFITVDEEGGMVARLANNDAFDLPRYASAAAVGASGNPENARAMGQ
                     TIGGYLKTYGFNMDFAPDADVNTNPDNPIIGERAFSSDAATAAQMAAAAAEGLRQNGI
                     LPTLKHFPGHGDTAEDSHTDLAVTYKTSEELENCELLPFAADTGLHAVMVGHIAAPNV
                     IGSDTPATLSAELIAMIPDTENTLIVTDSLSMDAITKAYTPGEAAIQAIQAGCDVLLM
                     PNSLPEAYDAVLAAVQNGTISEARLDKSVNKILHYKQQFAV"
     gene            1018..2070
                     /locus_tag="ACZPAX_09000"
     CDS             1018..2070
                     /locus_tag="ACZPAX_09000"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR1114074.1"
                     /translation="MSNTIWAVDGSFFAQRVSGIQRYSIELLAALDRIVPPDFVEIVT
                     PQGVTTPAYQNIRAVPFGTRRGLAWQQLDYPEYLKRRGAKGLATCNIIPWFGFRGIAV
                     VHDVCYRARRDFYTSGRDRLSAAWHCLQYRRIAQKAERIITVSEFSKSEIHKYYGVPL
                     EKMNIVYNAWQQMQRLTPDDGIFAKHPQLQRGAYYFSMANLLKNKNFPWVLRAAKAKP
                     DAVFAIAGGGSLAAEAERLGLADLPNVLYLGYVADGEAKALMAHCKAFLFPTLYEGFG
                     IPPLEAVACGAPEIIVSDTPCMREVYGDCAAYIDLSTNPGDVSAVTPPAAVPAVLLEK
                     YSWDKSAAKLLEILER"
     gene            2081..>2568
                     /locus_tag="ACZPAX_09005"
     CDS             2081..>2568
                     /locus_tag="ACZPAX_09005"
                     /inference="COORDINATES: protein motif:HMM:NF025639.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK-like family protein"
                     /protein_id="HIR1114075.1"
                     /translation="MTDEEALKKFLLDIDCLNELLPWTGKFNLFDVLKISRAEIRHSN
                     MLAWLLNANENHGMGDAYVKALVQKLVANDSAGRYDVFQLALMDFYSFTVQREWSDID
                     ILLTSSEEKTLIAFENKVGAHEHSDQLNRYRKILEKAYPDYCRIYLYLTPDGELPSDE
                     EY"
BASE COUNT          578 a          755 c          720 g          515 t
ORIGIN      
        1 agaccattga cgatcccagg gccgacggtg tgacccaagt gaccgacgcc atgcgggaga
       61 cgctgcagca atacccggtg ggcggcatct gccagtttgg caaaaatatc gagtcaccgt
      121 cccagctggc gcgcttcaat aaagacttgc aggccgccag cgccacgccg ctgttcatca
      181 cggtggatga ggagggcggc atggtcgcgc ggctggcgaa caacgacgcc tttgacctgc
      241 cccgctatgc cagtgccgcc gcagtgggtg catcgggcaa tcctgagaac gcccgcgcta
      301 tggggcagac catcggcggg tacctgaaaa cctacggctt caatatggac tttgcgcccg
      361 acgctgacgt gaacaccaac cccgataacc ccatcatcgg agagcgtgct ttctcgtcgg
      421 atgccgccac cgctgcccaa atggccgccg ctgcggcgga gggcctgcgg caaaacggca
      481 tcctgcccac gctcaagcat ttccccggcc acggcgatac tgccgaggac agccataccg
      541 acctggctgt gacctacaag accagcgaag aactggaaaa ctgcgaactg ctgccctttg
      601 cggcggatac cggcctgcac gctgtcatgg tggggcacat cgccgcgccg aacgtgatcg
      661 gcagcgacac ccccgcgacc ctctcggcgg agctgattgc gatgattccc gacacagaga
      721 acacgctgat cgtcaccgac tcactttcga tggacgccat cacaaaagcc tacactcccg
      781 gagaggctgc cattcaggcc atccaggcgg ggtgtgatgt acttttgatg ccaaactccc
      841 tgccggaagc ctacgatgct gtccttgccg ctgtgcaaaa cggcacgata tccgaagccc
      901 gccttgacaa aagcgtcaac aaaatcctgc attataaaca acaatttgca gtgtaggggc
      961 ggggctctgc tccgcccgca tctgatgcgc actattaatg tataaacgag gtaccctatg
     1021 tccaacacca tctgggccgt cgatggctct tttttcgctc agcgcgtctc gggcatccag
     1081 cgctattcca ttgagctgct ggccgccctg gatcgcattg tgccgcccga ttttgtggag
     1141 atcgtcaccc cgcagggcgt gaccaccccg gcctaccaga acatccgggc cgtgcccttc
     1201 ggcacccgcc ggggccttgc ctggcagcag ctggattacc ctgaatattt aaagcggcgc
     1261 ggggctaagg gccttgctac gtgcaacatt attccatggt tcggcttccg cggcattgca
     1321 gtcgttcacg atgtctgcta ccgtgcccgg cgcgattttt atacgtccgg ccgcgaccgc
     1381 ctgagcgccg cctggcactg cctgcaatac cgccgcattg cccaaaaagc cgagcgcatc
     1441 atcacggtgt cggagttttc caaatctgaa attcacaaat attatggtgt accgctggaa
     1501 aaaatgaaca tcgtctacaa cgcctggcag cagatgcagc gcctgacacc cgatgacggc
     1561 atttttgcca agcatccgca gctgcaaagg ggtgcgtact atttcagcat ggcgaacctg
     1621 ctgaaaaaca aaaacttccc ctgggtgctg cgcgcggcca aggcaaagcc cgacgcggta
     1681 tttgccattg cgggtggcgg tagcctggcc gccgaggccg agcgcctggg gctggccgac
     1741 ctgcccaacg tgctctacct cggctatgtc gccgacggtg aggccaaagc gctgatggcc
     1801 cactgcaaag cgttcctttt cccgacgctc tacgaggggt tcggtatccc gccgttggag
     1861 gccgtcgcct gcggtgcgcc tgagatcatc gtcagcgata ccccctgtat gcgcgaagtc
     1921 tacggcgact gcgccgccta tattgatttg agcaccaacc ccggcgatgt ttctgccgtc
     1981 accccgcccg ccgccgtccc tgccgtcctg ctggaaaaat acagctggga caaaagcgct
     2041 gcgaaactgc tggaaattct ggaaaggtag ggtgtaagca gtgacagacg aagaagcatt
     2101 gaaaaaattt ttgctggata ttgattgcct aaatgaactg ctgccgtgga caggaaaatt
     2161 taatctgttt gatgtgctga aaatttcgcg ggccgaaatt cgccacagca atatgctggc
     2221 gtggcttttg aacgcaaatg aaaatcacgg catgggtgat gcgtatgtaa aagcgctggt
     2281 gcaaaaactg gtggcgaatg atagtgctgg gcggtacgat gtgttccaac tggcattgat
     2341 ggatttttac agctttacag tacagaggga gtggagcgat atagatattt tgctcacctc
     2401 cagcgaagaa aaaacactga tagcatttga aaataaggtt ggtgcgcatg agcacagtga
     2461 ccaactgaac cggtatcgaa aaatccttga aaaagcgtac ccggactatt gcagaatcta
     2521 tctgtatctg acaccagacg gggaattgcc gagtgatgaa gagtactg
//
LOCUS       DBMDSQ010000287         2563 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_8961_length_2563_cov_1.154346, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000287 DBMDSQ010000000
VERSION     DBMDSQ010000287.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 2563)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2563)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2563
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8961_length_2563_cov_1.154346"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..689
                     /locus_tag="ACZPAX_09010"
     CDS             <1..689
                     /locus_tag="ACZPAX_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HIR1114076.1"
                     /translation="RGYSPGRFSFNVNGGRCEACKGDGQIKIEMHFLPDVYVPCEVCG
                     GARYNRETLQVTYRGKNISEVLDMTVEDALAFFENIPGIKRKLQTLFDVGLGYIRLGQ
                     PATTLSGGEAQRVKLASELQRRQTGKTFYILDEPTTGLHVADVHRLVKVLDKLVDAGN
                     TVIVIEHNLDVIKRADYIIDLGPEGGSGGGTIVATGTPEEVAQNPNSFTGQYLKPVLE
                     RAAELQQSQK"
     gene            832..1758
                     /locus_tag="ACZPAX_09015"
     CDS             832..1758
                     /locus_tag="ACZPAX_09015"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HIR1114077.1"
                     /translation="MTVLRYTVPPENEGCKLGLFLRMQGVTAGLIKSVKHEGSGFFAD
                     DDPLHTNQPVHTGQCISFALPPEHDTSVTPQPVPFSIAYEDEFAAVLNKPAGIAVHPT
                     LNYPDGTLANGWLYHLQQEGCTGIFRPVNRIDKNTSGLVLCAQNAFAAPLLAASAHKC
                     YLAIVQGAMPLGRGRVEAPIARRGDSIIGRCVREDGKYSLTEYQVLAVGRSHSLVACW
                     LCTGRTHQIRVHMAYIGHPLAGDTLYGGSDAVIARHALHCAILHFAHPLTGKPIRVEC
                     PLPADMAALAETENLLDGWQLSSLPCGEDALT"
     gene            1792..>2563
                     /locus_tag="ACZPAX_09020"
     CDS             1792..>2563
                     /locus_tag="ACZPAX_09020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1114078.1"
                     /translation="MNPIQTDATPKSENLAGQSESAENVRFAHSSQEKKRNENRRKAS
                     RYMKDLQRRSVVGDMLYMVGFWVEYVFVCVGRRLGAVLHGVADTALNLLLIVLRPILI
                     GLITLVEDLTSPFVRMASGIRHIQALPDTMEESEAGTVRAAKAKYLASGIRKYAIVVW
                     NAITYILPAVAAAGLVVIVRGGLGLKFVLNVQVNGENVGYVESEQVFESAREDVQSRI
                     NTAKRMLQNSGSDITDTNWDVYPTYSLSIGNQTMTESEI"
BASE COUNT          508 a          789 c          778 g          488 t
ORIGIN      
        1 cgcgcggcta cagccccggg cgcttcagct tcaacgtcaa cggcggacgc tgcgaggcgt
       61 gcaagggcga cggccagatc aagatcgaga tgcacttcct ccccgacgtc tacgtgccct
      121 gcgaggtgtg cgggggggcg cgctacaacc gcgagacgct gcaggtcacc taccgcggca
      181 agaacatctc ggaggtgctc gacatgacgg tggaggacgc gctggcgttc ttcgagaaca
      241 tccccggcat caagcgcaag ctgcagacgc tgttcgacgt cggcctgggc tacatccgcc
      301 tgggccagcc cgcgacgacg ctctcgggcg gcgaggcgca gcgcgtcaag ctcgccagcg
      361 agctgcagcg ccgccagact ggcaagacgt tctacatcct ggacgagccg accaccggcc
      421 tgcacgtcgc ggatgtccac cgcctggtca aggtcctgga caagcttgtc gatgcgggta
      481 acacggtcat cgtcatcgaa cacaatctcg acgtcattaa gcgtgctgac tatatcatcg
      541 accttggccc cgagggcggc agcggcggcg gtaccatcgt cgcgaccggc acgcctgagg
      601 aagtcgcgca aaatcccaac tccttcaccg ggcaatacct gaaacccgtg ctggaacgcg
      661 cggcagaatt gcagcaatca caaaaataac acagacggct ttcctgcggg aaagccgttt
      721 tctttgttgt gccgctgttt ttctgcctgc gcattgccag acattggggt ttgcatcaca
      781 gcacgttttg tggtataata atcgcatata ggctttgagg agggcggcgc tgtgaccgtt
      841 ttgcgatata ctgtgccgcc ggagaacgag ggctgcaagc tggggctgtt cctgcgaatg
      901 cagggcgtca cggcagggct gatcaagtcc gtcaagcacg agggcagcgg cttttttgcc
      961 gatgatgacc ccctgcacac caaccagccg gtgcacaccg ggcagtgtat ttcctttgcg
     1021 ctgccaccgg aacatgacac atcggtgacg ccgcaaccgg taccgttttc catcgcctat
     1081 gaagatgaat ttgccgcagt gctcaacaag cctgcgggca tcgccgtaca ccccacgctg
     1141 aactacccgg atggcacgct ggccaacggc tggctgtacc atctgcagca ggaggggtgc
     1201 accggcattt tccgccctgt caaccgcatt gacaaaaaca ccagcggtct tgtgctctgc
     1261 gcccagaacg cctttgccgc accgttgctg gctgcctcgg ctcacaagtg ctaccttgcc
     1321 attgtgcagg gggcaatgcc attgggccgc ggcagggtag aggcccccat tgcgcggcgg
     1381 ggtgattcca tcattggccg ctgcgtgcgg gaggacggca agtacagcct gacggaatat
     1441 caggtgctgg ccgtgggccg cagccacagt ttggtggcct gctggctctg caccgggcgc
     1501 actcatcaga tccgcgtcca tatggcgtat atcggccacc cgctggcggg ggacaccctc
     1561 tacggcggct cggatgcagt cattgcccgc cacgcactgc actgcgccat cctgcatttt
     1621 gcccatccgc tgacaggcaa gcctatccgg gtcgagtgcc cgctgcccgc cgacatggcc
     1681 gccctggccg agactgaaaa cctgctggac ggctggcagc tgagcagtct gccctgcggc
     1741 gaagatgcac ttacttaaac tttgttacac acggaaagga gcttccgtat tttgaatccc
     1801 atccaaaccg atgcgacgcc aaagagtgaa aacctcgccg gtcagtcgga aagcgcggaa
     1861 aatgtgcgct ttgctcattc ctcccaggag aaaaaacgca acgagaaccg ccgcaaggca
     1921 tcccgctaca tgaaagattt gcagcgccgc agtgttgtcg gcgatatgct gtacatggtc
     1981 ggtttctggg tcgagtatgt ctttgtctgc gtcggccgca ggctgggcgc tgtactgcac
     2041 ggtgtcgccg ataccgcgct gaacctgctg cttattgtgc tgcgcccgat tttgattggg
     2101 ctaatcacgc tggtggagga tctgaccagt cccttcgtgc gcatggcgtc gggcatccgc
     2161 cacatccaag ccctgccgga cacgatggag gagagcgagg ccggcactgt ccgtgccgcc
     2221 aaggccaagt atcttgccag cggcatccgc aaatatgcca tcgtcgtatg gaacgcgatt
     2281 acatacatcc tgcccgccgt ggcagctgcg ggcctggtgg tcattgtgcg cggcggcctg
     2341 ggcctgaagt tcgtgctgaa cgtgcaggtg aacggcgaaa acgtcggcta tgtggagagc
     2401 gagcaggtgt ttgagagcgc ccgcgaggat gtgcagtcgc gtatcaacac cgccaagcgt
     2461 atgctgcaaa attccggctc ggacatcacc gacacaaact gggacgtcta cccgacctat
     2521 tcgctgtcca tcggcaacca gacaatgacc gagagcgaga ttg
//
LOCUS       DBMDSQ010000288         2555 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_8989_length_2555_cov_2.368786, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000288 DBMDSQ010000000
VERSION     DBMDSQ010000288.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 2555)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2555)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2555
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8989_length_2555_cov_2.368786"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..349
                     /locus_tag="ACZPAX_09025"
     CDS             <1..349
                     /locus_tag="ACZPAX_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009324068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HIR1114079.1"
                     /translation="TVITNAGRLRLKESDRIEAMRDELQKMGARIEVDGDTVHIQGVA
                     LHAPNAPLSGHNDHRIVMALAVAAIASGLPALLCGAEAVDKSWPAFWNVLRGLGAKVE
                     LSTDKTNEFDMKA"
     gene            356..1210
                     /locus_tag="ACZPAX_09030"
     CDS             356..1210
                     /locus_tag="ACZPAX_09030"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007045998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HIR1114080.1"
                     /translation="MLDKSKNYLIVGLGLLGGKYAQVLSQKGYNVTGITHSQATLDYA
                     LQHDYIRAGKNADFEDLIRAADCIVFGLYPTILLEWVAQYGQYIRPGTMVTDVSGVKR
                     GVVEPVQAALPDGVEFIASHPMAGRETSGIAHSAEVNFAPANFIITPTEKNTQAGIDW
                     CRDLAEALGFKRISVLTPAEHDHMIGYVSQLCHAIAVSLMCAADNSELANYTGDSFRD
                     LTRIARINDKMWAELFLWNKDNLISEIDMFDSALQDMKEKLVNDDREGLEEMFRLSTK
                     RREAFDKK"
     gene            complement(1663..2349)
                     /locus_tag="ACZPAX_09035"
     CDS             complement(1663..2349)
                     /locus_tag="ACZPAX_09035"
                     /inference="COORDINATES: protein motif:HMM:NF039407.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6320 domain-containing protein"
                     /protein_id="HIR1114081.1"
                     /translation="MNTCPHCHIQVGGDARYCPLCQNRLPGSAGEPYFPATAPRIHRA
                     SLLYKIVAFVLSALVVVGGAFDFLLLEETPHRHFSVLMAVWAVALLRVLRAVLRRRFN
                     GPRQIFNLLLLVSALLVFTDWFNGYTGYSLDLVVPILCCVALACNFIFAFLRSNFTAN
                     ALVYLLMNIGIGVLPYILLFFRIDYDGKLDAHSIPWVICLILSIITFLGLVIFRGRDL
                     KNELVKRLHM"
     gene            complement(2346..>2555)
                     /locus_tag="ACZPAX_09040"
     CDS             complement(2346..>2555)
                     /locus_tag="ACZPAX_09040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1114082.1"
                     /translation="MPEELRPYIHSFAAFSSCKTLFTVVCSYGDDLVLGTASALRSTA
                     ILQRFYRELTKAGLDVTLYATEVEK"
BASE COUNT          612 a          707 c          747 g          489 t
ORIGIN      
        1 aacagtcatc accaacgcgg gccgtctgcg cctgaaagag tccgacagaa tcgaagctat
       61 gcgggatgag ctgcaaaaga tgggcgcacg cattgaggtg gacggcgaca ccgtgcatat
      121 tcagggcgtg gcgctccacg cgccgaacgc gccgctgtct ggccataacg accaccgcat
      181 tgtcatggca ctggccgtgg cagccattgc cagcggcctg cctgctctgc tctgcggggc
      241 cgaggctgtg gacaagagct ggcccgcgtt ctggaacgtg ttgcgcggct tgggtgccaa
      301 ggtggagctt tccacggaca aaacgaatga atttgacatg aaggcgtgat tttgtatgct
      361 ggacaaaagc aagaactatc tgattgtcgg cctgggactg ctgggcggca aatacgccca
      421 ggtgttgagc cagaagggtt acaacgtgac cggcattacc cacagccagg cgacgctgga
      481 ctatgccttg cagcatgact atatccgcgc aggcaaaaac gcggattttg aggatttgat
      541 ccgggcggcc gactgcatcg tgttcggcct ctatcccacc attttgctgg aatgggtcgc
      601 gcagtacggc caatatatcc gccccggcac aatggtgacg gacgtctccg gcgtcaagcg
      661 cggcgtggtt gagcctgtgc aggctgcttt gcctgacggg gtagagttca tcgccagcca
      721 cccgatggca ggccgcgaga cgagcggcat tgcccacagt gccgaggtca actttgcccc
      781 ggccaacttc atcatcacgc cgacggagaa aaacacccag gcgggcatcg actggtgccg
      841 cgatctggct gaggcgctgg gcttcaagcg aatcagcgta ctgacccccg ccgagcacga
      901 ccacatgatc ggctatgtca gccagctgtg ccacgcgatc gccgtcagcc tgatgtgtgc
      961 ggcggataac tccgaacttg cgaattacac cggcgattcc ttccgggatt tgacaagaat
     1021 tgcgcgtatc aacgacaaaa tgtgggcgga gttgtttttg tggaacaagg acaacctgat
     1081 ttcggagatc gatatgtttg acagcgcctt gcaggatatg aaggaaaagc tcgtcaacga
     1141 cgaccgggaa ggcctggaag agatgttccg cctgtccacc aagcgccgcg aagcgtttga
     1201 taagaaataa gcgttgacga aacgaaagat ttgtgggata acataaggca ataccgcaca
     1261 attcccgcct tacggcttaa gcaccgctcc ggcggctgcg gcacggcatc tgcgttgcca
     1321 aaacgctcga taagacatcc agtattatct gcgcttttgg cttagcagct gccgcacctc
     1381 actcgccgta tcggcactta gaattatgcg gtattgccat aaatagaaaa ggcactgcga
     1441 caagcggttg gcttttagta acaggaacca gataactcta agaaccgtca cttggccgag
     1501 tggcggttct tgttttttac aacagaaaac gacaaaaagc aagtacatcg gacaaaatcc
     1561 ggcgtacttg cttttctttt tgcatgttta tacgttgatt tttctgccca cgcgcagtac
     1621 cagcacccca atcactaccc tcccctacaa ctggggataa aatcacatat gcagtctctt
     1681 gaccagttcg tttttcaagt cgcggccgcg gaagatgacc agccccaaaa atgtgatgat
     1741 gctcaaaatc aggcagatga cccaggggat gctgtgggcg tccagcttac cgtcgtaatc
     1801 aatgcggaag aacagtaaaa tatacggcag aacgccgata ccaatgttca tgagcaggta
     1861 caccagtgcg ttggcggtga aattgctgcg caagaacgca aaaataaagt tgcaggccag
     1921 cgccacacag cacaaaatcg gcacgactag atcaaggctg tacccggtgt agccgttgaa
     1981 ccagtcggta aagaccaaca gcgccgagac gagcagcagc aggttgaaaa tctggcgcgg
     2041 gccgttgaat cggcggcgca gcacggcgcg aagtacccgc aggagggcga cggcccagac
     2101 ggccatcagc acactgaaat ggcggtgggg tgtttcttcc agcagcagaa agtcgaatgc
     2161 gccgcccacg acgacaagcg ccgacagcac gaacgccacg attttgtaca gcagcgacgc
     2221 gcggtggatg cgcggcgcgg tggccgggaa gtatggctcc cccgccgagc cgggcaggcg
     2281 gttctggcac agcgggcagt atcgggcatc cccaccgacc tggatatgac agtgcggaca
     2341 ggtattcatt tctccacctc cgtggcatag agcgtgacat ccagcccggc cttggtcagc
     2401 tcgcggtaga accgctgcaa aatggccgtg ctgcgcagcg ccgatgccgt acccagcaca
     2461 aggtcgtcgc cgtaggagca gacgaccgtg aacagcgttt tgcagctgga aaacgctgca
     2521 aagctgtgga tgtagggtct cagttcctcg ggcat
//
LOCUS       DBMDSQ010000289         2553 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_8998_length_2553_cov_1.758972, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000289 DBMDSQ010000000
VERSION     DBMDSQ010000289.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 2553)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2553)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2553
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8998_length_2553_cov_1.758972"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1030)
                     /locus_tag="ACZPAX_09045"
     CDS             complement(<1..1030)
                     /locus_tag="ACZPAX_09045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1114083.1"
                     /translation="MSDSWYKVDNVAKVFLATASQRDPRVFRVSCTLQEDIDPDTLNT
                     ALRATAQEWPQFQVTLHRGLFWHYFESTDLVPTAQPENKAPCAPLYTPERRNKLIYRV
                     TYFGARINLEMFHAITDGNGGLLYLKSIVRNYLALRHPGQLDDVPSPNSASAADLVQD
                     SFRNFYGGTKRDNAAKKVKAYRPRGLRLPYDQLQFFEGHLSAKQVLERCHALGVSMTS
                     YLGASFMLAIYHDMPALERKKPICISLPVNLRNYYPSETARNFFNSVYVTHTLTDADT
                     LETVAPVFDAKLKEVLKPENIRAQMDEFEKLEHMPGIRPVPLVVKNLTVKFFTALEKK
                     HVTAVISNM"
     gene            complement(1094..2071)
                     /locus_tag="ACZPAX_09050"
     CDS             complement(1094..2071)
                     /locus_tag="ACZPAX_09050"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007045995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR1114084.1"
                     /translation="MPRKKTDLSQRLEAVIKTLVPQQEQNPDDLGPMMRAIKAVHSIS
                     DHTSTTPEDLEHQRAAQELFGRLVTPSIGIRNDSLTVGSIPAEWVSLEHGHDRHHAVL
                     YCHGGGYTCGQLGYARILASKLALATGFDVLSFEYRLAPEHPFPAAIEDAVAMWDYLM
                     YMGYGARDVIIAGDSAGGNLALELALKLKEQGRAQPRGLLLFSPWTDMTASGKSYRTC
                     KTLDPMLTMEYIAAVREAYTGPDADWSRPCYSPLFADLRGLPPVLVQVGTNEILKSDS
                     EQLVENLQKAGVYAQLEVYPECWHVFQQMPIRRAAVAMESAGRFVQKII"
     gene            complement(2081..>2553)
                     /locus_tag="ACZPAX_09055"
     CDS             complement(2081..>2553)
                     /locus_tag="ACZPAX_09055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1114085.1"
                     /translation="DAVESGGAPHSLTHFGGELVAGSKARLADFMALCQQYFDELQAR
                     GITPKEGDEAVWCGAAYRSLRAGQPVRAANAYIFRYWLGGRFYYVSTNYQLDPVCILH
                     LPGAAKDRQLKLIYKKYTKRGAFPPPDAIHRLCGLPAAHPPLLRTLWTRLLAKL"
BASE COUNT          474 a          740 c          817 g          522 t
ORIGIN      
        1 ccatgttgga gatgaccgct gtgacgtgct ttttctccag cgcggtaaaa aactttacgg
       61 tcaggttttt gacaaccagc ggcaccgggc ggatgccggg catatgttcg agcttttcga
      121 actcgtccat ctgggcgcga atgttctcgg gttttaagac ctctttcagc ttggcatcaa
      181 acaccggggc gacggtttcc agcgtgtcag catcggtcag cgtgtgggtg acatagaccg
      241 agttgaagaa gttgcgggcc gtctcgctgg ggtagtagtt gcgcaggttg accggcagac
      301 tgatacagat aggcttttta cgttccagcg cgggcatatc gtgatagatc gccagcatga
      361 atgacgcgcc gaggtagctt gtcatcgaga cgcctagcgc gtggcagcgt tccagcacct
      421 gtttggcgct cagatgccct tcaaaaaatt gcagctggtc gtagggcagg cgcagtccgc
      481 gcgggcggta ggccttgact tttttggccg cattatcgcg ctttgtgccg ccgtaaaagt
      541 tgcggaagct gtcctgcacc aagtcggccg ccgaagcgct gttgggcgaa ggtacgtcgt
      601 ccagctggcc ggggtggcgc agggccagat agttgcgcac gatggatttt aagtagagca
      661 gcccgccgtt gccgtcggta atggcgtgga acatttccag gttgatgcgt gcgccgaaat
      721 aggtcacgcg gtagatgagt ttattgcggc gctccggcgt gtacagcggc gcacaggggg
      781 ccttattctc cggctgggcc gtggggacaa ggtcggtgga ctcaaaataa tgccagaaca
      841 aaccgcggtg cagtgtcacc tggaactgcg gccattcctg cgcggtggcg cgcagggcgg
      901 tgttcagcgt gtcgggatcg atgtcctcct gcagtgtgca ggagacgcgg aacacccgcg
      961 ggtcgcgctg ggacgccgta gccaggaaca ccttggcgac gttatcaact ttgtaccatg
     1021 aatcggacat gtttttcctt tctctgctaa aggattaagg ggaggctccc gggaggtgtt
     1081 gtattatgtt tcctcaaata atcttctgca caaatcgccc cgcgctctcc atcgcgacgg
     1141 cggcgcggcg gatgggcatt tgctggaaaa cgtgccagca ctcggggtaa acttccagct
     1201 gggcgtagac cccggctttc tgtaaatttt cgaccaactg ttcactgtcg gacttcaaaa
     1261 tttcgtttgt gcccacctgc acaagcacgg gcggcaggcc gcgcaggtcg gcaaacagcg
     1321 ggctgtaaca cgggcgggac caatcggcat cggggccggt gtaggcttcg cgcacggcgg
     1381 cgatgtactc catcgtcagc atcgggtcca gcgttttgca ggtgcggtag ctcttgccgc
     1441 tggccgtcat gtcggtccag gggctgaaca gcaaaagccc ccgcggctgg gcgcggccct
     1501 gctctttcag ctttaacgcc agctccaaag ccaggttgcc cccggcagaa tcgcccgcaa
     1561 taatcacgtc ccgcgcaccg tagcccatgt acatcaggta atcccacatg gcaacggcgt
     1621 cctcaatagc ggcggggaac ggatgctcgg gcgcaaggcg atactcaaac gagagcacgt
     1681 caaacccggt ggcaagcgcc aacttgctgg ccagaatgcg ggcatagccc agctggccgc
     1741 aggtgtagcc gccgccgtgg cagtacaaaa ccgcgtggtg gcggtcgtgg ccgtgctcca
     1801 ggctgaccca ctcggcgggg atactgccca ccgtcaggct gtcattgcgg atgccgatgc
     1861 ttggcgtgac cagccgccca aacaactcct gcgcggcgcg ctggtgctcc agatcctccg
     1921 gcgtggtcga ggtatggtcg ctgatgctgt gtacggcctt gatggcccgc atcatagggc
     1981 cgaggtcgtc ggggttctgc tcctgctggg gcaccagcgt cttgatgaca gcctccaaac
     2041 gctggctgag gtcggtcttt tttctgggca tagcggctcc ttacagcttg gccagcagcc
     2101 gcgtccaaag ggtgcgcagc agcggcggat gggcggcggg cagaccgcac aggcggtgga
     2161 tcgcgtccgg cggcgggaac gccccgcgct tcgtgtactt tttatagatc agtttcagct
     2221 ggcggtcctt ggctgccccg ggcagatgca ggatgcatac cgggtcaagc tgataattcg
     2281 tcgatacata gtaaaaacga ccgcccagcc agtagcggaa aatataggcg ttggcggcac
     2341 gcaccggctg ccctgcccgc agactgcggt aggccgcgcc gcaccagacg gcctcgtcac
     2401 cctccttggg ggtgatgccc cgggcctgca attcgtcaaa atattgctgg cacagcgcca
     2461 taaaatcagc caaccgggcc ttgctgcccg ccaccagttc gccgccgaaa tgcgttaagc
     2521 tgtggggtgc accgcccgat tctaccgcgt cat
//
LOCUS       DBMDSQ010000290         2552 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_9004_length_2552_cov_1.440636, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000290 DBMDSQ010000000
VERSION     DBMDSQ010000290.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 2552)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2552)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2552
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9004_length_2552_cov_1.440636"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(88..178)
                     /locus_tag="ACZPAX_09060"
     tRNA            complement(88..178)
                     /locus_tag="ACZPAX_09060"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(142..144),aa:Ser,seq:tga)
     gene            complement(256..378)
                     /locus_tag="ACZPAX_09065"
     CDS             complement(256..378)
                     /locus_tag="ACZPAX_09065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1114086.1"
                     /translation="MKRIAALTLTAVLCFGALTACSAAKDTTPCAPRWKMRNKI"
     gene            complement(498..1325)
                     /locus_tag="ACZPAX_09070"
     CDS             complement(498..1325)
                     /locus_tag="ACZPAX_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HIR1114087.1"
                     /translation="MAGEYLKSSVHVHSKLCDGKNTPEEIAVTAWRAGLQTLGFSGHS
                     HTPHDLEYCMTQSRTALYKAQVAKLKERYAGKLDILCGLEWDLYSDDDPTQYDYWIGS
                     AHYVKGPKTGKYYEIDWREEDLRACIDDDFDGDALAVVEAYFANVAKVAEKKPTILGH
                     FDLIKKINGDGKFFDENDPRYTAAANAALMTAARNRCVLEVNTSAAYRGFRKDYFPSD
                     AILKDWLILSGNVVITADAHDAKALTYGFEEAAAKLKELGYTKVQVLGKDGFIPCAL"
     gene            complement(1329..1985)
                     /gene="hisIE"
                     /locus_tag="ACZPAX_09075"
     CDS             complement(1329..1985)
                     /gene="hisIE"
                     /locus_tag="ACZPAX_09075"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HIR1114088.1"
                     /translation="MEITENSKTLKFDANGLIPAIVQDHYTKEVLTLAYMNAETLALT
                     IAEGRTVFWSRSRQEVWRKGDTSGNVQRVVSITADCDKDALVIDVVKSGPACHTGAES
                     CFFNPVYVSDELKQFTWQGLYELIEGRKTNPKEGSYTTYLFDKGLEKILKKVGEESTE
                     VIIAGAKRDKEETIYEISDLAYHVMVLMIELGISVEDITKELEKRHVIDHKVKQERMQ
                     "
     gene            complement(2187..>2552)
                     /locus_tag="ACZPAX_09080"
     CDS             complement(2187..>2552)
                     /locus_tag="ACZPAX_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015537578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HIR1114089.1"
                     /translation="KRVDGKFMLFAKGGRENTGIDALSWLEQGVKNGAGELVVNSIDT
                     DGVKNGFDLELLDAVAARCAVPIIASGGAGKKEDFLELFRNHPAVDAGLAASIFHTKQ
                     VEINNLKKYLRTNGVEMRV"
BASE COUNT          504 a          688 c          738 g          622 t
ORIGIN      
        1 acgcccttag caggggcgcc tcttcgacct cttgagtatt actccacaag tcaaagtgat
       61 ataaaatatt cacttgttat tacaaaatgg cggagaggat gggattcgaa cccatggtcc
      121 gctcgcgcga atcgctggtt ttcaagacca gttccataaa ccactcggac acctctccac
      181 agtggctgcc gccagaatgc aggtattatt atacaaaaaa gcggaggggt tgtcaaccct
      241 tccgtgcaaa cttttttaaa ttttattccg cattttccag cgcggtgcgc acggcgtggt
      301 atccttggcg gcagagcagg cggtcagtgc gccaaagcag agaacggcgg tcaaagtcaa
      361 agcggcaata cgtttcatgg taaaaaccct tttgaaattc ggttggtttc aaaatatcca
      421 gttcagaggt cgctcactca tagaaacgca aaagccgccg cagtgttaca ctgcggcggg
      481 aaattttaaa aaatatttta cagtgcgcag gggatgaagc cgtccttacc cagtacctgc
      541 accttggtgt agcccagctc tttcagcttg gcggcggctt cctcaaagcc gtaggtcagc
      601 gccttggcgt cgtgggcgtc ggcggtgatg accacgttgc cggacagaat gagccaatcc
      661 ttcaggatgg cgtcggaggg gaaataatcc ttgcggaagc cgcggtacgc tgcggaggtg
      721 ttgacctcca gtacgcagcg gttgcgggcg gcggtcatca gggcggcgtt ggcagcagcg
      781 gtgtagcgcg ggtcgttctc gtcaaagaac ttgccgtcgc cgttgatttt tttaatcagg
      841 tcaaaatggc ccaaaatcgt cggtttcttc tcggcgacct tggccacatt ggcaaagtat
      901 gcctcgacca cggcgagcgc gtcaccgtcg aaatcgtcgt cgatgcaggc gcgcagatcc
      961 tcctcgcgcc agtcgatttc ataatatttg ccggtcttgg ggcctttgac atagtgggcg
     1021 ctgccaatcc agtagtcgta ctgggtcggg tcgtcatcgc tgtacaagtc ccattccagg
     1081 ccgcacagaa tgtccagctt accggcatag cgctctttca gcttggcgac ctgcgccttg
     1141 tacagggcgg tgcggctctg ggtcatgcag tattccaggt cgtggggggt gtggctgtgg
     1201 ccgctgaagc ccagcgtctg cagcccggcg cgccatgcgg tgactgcgat ctcctcgggg
     1261 gtgtttttgc cgtcgcacaa tttggaatga acgtgtacag agcttttcag gtattcgcca
     1321 gccatagttt actgcatcct ttcctgcttg accttgtggt cgataacgtg tcgtttttcg
     1381 agttctttgg tgatgtcctc aacagaaatg cccagctcga tcatcagcac catgacgtgg
     1441 taggccaggt cgctgatttc gtagatggtc tcttctttgt cgcgctttgc gcccgcaatg
     1501 atgacttcgg tggattcctc gccgactttt ttgagaatct tctccaagcc cttgtcgaac
     1561 agatacgtgg tgtagctgcc ctccttgggg ttggtcttgc ggccctcgat cagctcatac
     1621 aggccctgcc aggtgaactg tttcagctcg tcggagacgt agaccgggtt gaagaagcag
     1681 ctctccgcgc cggtgtggca ggcggggcca gactttacaa cgtcgatgac gagggcatcc
     1741 ttgtcgcagt cggcggtgat ggagacgacc cgctgcacat tgccggaagt atcgccctta
     1801 cgccagactt cctggcggct gcgggaccag aacacggtgc ggccctcggc aatggtcagg
     1861 gccagcgtct cggcgttcat gtacgcgagg gtcaatacct ctttggtata atgatcctgc
     1921 acgatggcgg ggatcaggcc gtttgcgtca aatttgaggg tcttggaatt ttcggtaatt
     1981 tccatgtcgg acactcctaa tgtaatatag tttagtgtac ctctttttgt gggttttggc
     2041 aagaggtaaa gtgtaggttt gctatagtta cgcagtaggg gccgggcatg cccggcccgc
     2101 ggctttcccg caagaggacg cttttgggaa tgttgcgggc cgcacatgtg cggcccctac
     2161 cgggtggctg catggcgaag ggggaatcac acccgcatct ctacgccgtt cgtgcgcaga
     2221 tatttcttca aattgttgat ttccacctgc ttcgtgtgga aaatcgacgc ggccagcccg
     2281 gcgtccacgg cagggtgatt gcggaacaat tcaaggaaat cctctttttt gcccgcgccg
     2341 ccggaggcaa tgatgggcac ggcacagcgg gcagccacgg cgtccagcag ctccaaatca
     2401 aagccattct tgacgccgtc ggtgtcgatg gagttgacga ccagctctcc tgcgccgttt
     2461 ttgacgccct gctcgagcca gctaagcgca tcgatgccgg tgttctcgcg cccgcccttg
     2521 gcaaacaaca taaatttgcc gtccacacgt tt
//
LOCUS       DBMDSQ010000291         2498 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_9230_length_2498_cov_1.778056, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000291 DBMDSQ010000000
VERSION     DBMDSQ010000291.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 2498)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2498)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2498
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9230_length_2498_cov_1.778056"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(114..827)
                     /gene="purC"
                     /locus_tag="ACZPAX_09085"
     CDS             complement(114..827)
                     /gene="purC"
                     /locus_tag="ACZPAX_09085"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HIR1114090.1"
                     /translation="MNYEVKEQLYEGKAKQVFATNDPEIVMVHYKDDATAGDGEKKGT
                     IRDKGIVNNKLSNALMQKLEKEGIPTHYVKEVNDRDTFVKKVDIVPLEVIIRNVAAGH
                     FSLRMGVPEGKVFKTPILEYSYKNDDLHDPFINHYYALALDLATQEELDTIAKYAFKV
                     NEVLKKIMLDANIRLVDFKLEFGRLSDGTIILADEISPDTCRFWDATTGAHLDKDLFR
                     RNMGGEADAYQEVMKRLLG"
     gene            complement(881..1378)
                     /gene="purE"
                     /locus_tag="ACZPAX_09090"
     CDS             complement(881..1378)
                     /gene="purE"
                     /locus_tag="ACZPAX_09090"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002851000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HIR1114091.1"
                     /translation="MKKVAIIMGSDSDWPVVKSACTVLKDFGVPYEAHILSAHRTPEA
                     AAAFAKSARANGYGVILCAAGMAAHLAGAFAANTTLPVVGIPMKGGAMDGLDALLATV
                     QMPSGFPVATVALNGAKNAAYLAVAILAVADDELAEKLDKFRADTAAAIAKKDAAIAA
                     EAAAL"
     gene            1772..2275
                     /locus_tag="ACZPAX_09095"
     CDS             1772..2275
                     /locus_tag="ACZPAX_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HIR1114092.1"
                     /translation="MSEPRRRLTRRALHGREEWFKRVFRDQIAEAAGILEVVLSGIVL
                     VALLLSIVPLVELMPGLLTDAESIEVHTFLERALDIVIGIEFIKMLAKHSPGSVLEVL
                     LYAIARHMIVGHEDAVQNLVSVGAIALIFIIRKYFFVPSFGQKLPGGETAPDMKKAPE
                     ECPEADA"
BASE COUNT          535 a          659 c          734 g          570 t
ORIGIN      
        1 agtgtattgg gaaaattcgc tcatgttttg ctccgtttgt atgttgtaga gggcggcgtc
       61 cccgacgccc cgcggcagat accgccctac gcgtcacccg aattgtcgga aaatcagccc
      121 agcagccgct tcatgacttc ctggtaggca tcggcctcgc cgcccatgtt gcggcggaac
      181 agatccttat ccaggtgtgc gccggtggtg gcatcccaga aacggcaggt gtcagggctg
      241 atttcgtcag ccaggatgat ggtgccgtcg gacaggcggc caaactcgag cttgaagtcg
      301 accagacgga tgttggcgtc cagcatgatc ttcttcagca cttcgttgac cttgaaggcg
      361 tatttggcga tggtgtccag ctcttcctgg gtagccaggt cgagggccag ggcgtagtag
      421 tggttgatga acgggtcatg caggtcgtcg ttcttgtagc tgtactcgag gatgggggtc
      481 ttgaacacct tgccctcggg tacgcccatg cgcaggctga aatgaccggc ggcgacattg
      541 cggatgatga cttccagcgg gacgatgtct accttcttga cgaaagtatc acggtcgttg
      601 acttccttaa cgtaatgggt ggggatgccc tctttctcca gcttctgcat cagagcgttg
      661 gacagtttat tattgacgat gcccttgtcg cggatggtac ccttcttctc gccgtcgcct
      721 gcggtggcgt cgtccttgta gtggaccatg acgatttcgg ggtcattggt agcgaaaacc
      781 tgcttggcct tgccttcgta cagttgctct ttaacttcgt aattcatgaa aaacgctcct
      841 tttactttat tataatatgg ttgtggttgg tatgcaagtg ttacagcgcc gcagcctcgg
      901 cggcaatggc ggcgtccttc ttggcaatcg cggcggcggt gtcggcgcgg aatttatcga
      961 gcttttccgc cagctcgtcg tcggcaacag ccaggatcgc cacggccagg taggcggcgt
     1021 tcttggcacc gttcagcgcc acggtggcga ccgggaaacc gctgggcatc tgcacagtgg
     1081 caagcaatgc gtccaggccg tccatcgcgc cgcccttcat cgggatgccg acgaccggca
     1141 gggtggtgtt ggcggcaaag gcaccggcca ggtgtgcggc cataccggca gcgcacaaaa
     1201 tcacgccgta cccgttggcg cgggcagact tggcaaacgc ggcggcagcc tccggcgtgc
     1261 ggtgggcgga aagaatgtgt gcctcataag gcacgccgaa atccttcagt accgtgcagg
     1321 cggatttgac caccggccaa tcactgtcgg aacccatgat gatcgctact tttttcatgc
     1381 gtttagaaac ctcctttacg aagcaaaaca gcgcagtgcc ccgttaggat acactgcgct
     1441 gcggttttga tgtgaaaaga gtatgacggt acagaacacc cacgtgaaaa ccaagacgct
     1501 gcattgcgtt tgccacggaa gatccccctt taatataaaa gtaagatgat gccctgtgaa
     1561 accagtgtaa actctgggaa aagaaattgc aagcgtccgg cggacatttc agccaaaaaa
     1621 ctatgccgtc cggttttgac cggtgctttt tgtcgctttt gatggagttt tgaaggtgat
     1681 tgtcatactt taaaacggcc tgtataatta tgctggtata tcggggcaaa atatgctatg
     1741 ataataaaaa tagcacaaaa aggagaaagt tatgagtgaa cccagacgac gcctgacacg
     1801 ccgcgccctg cacggcaggg aggaatggtt caagcgcgtt ttccgcgatc agatcgccga
     1861 ggccgctggt attttggagg ttgtgctctc cggcattgtg ctggtggccc tgctgctcag
     1921 catcgtcccg ctggtggagc tgatgccggg tcttttgacc gacgcggaaa gcatagaggt
     1981 acatacgttc ttggaacgtg cgctggacat cgttatcggc atcgaattca tcaagatgct
     2041 ggcaaagcac agccccggca gcgtgctgga agtgctgctt tacgccatcg cccgccatat
     2101 gattgtcggc cacgaggacg ccgtgcagaa cctcgtcagc gtcggtgcca tcgcgctgat
     2161 tttcatcatc cgcaaatatt tctttgtgcc gtccttcggc cagaagctac ccggcggcga
     2221 gaccgcaccg gatatgaaaa aggcaccaga ggaatgcccg gaagccgatg cctgacaccg
     2281 ccacgccacg ggcatagaat acccggtctg gaaagagcag ggaaaaggtg tggcaaatgt
     2341 ccccatgggc ggacttgaag cccgccccta cggtataatg aacaaagcgg gcatccgttt
     2401 ggatacccgc tttgcttgat tgatgtaata agttagggca ataccgcata attctaagtg
     2461 ccgatacggc gagcgaggtg cggcagctgc taagccaa
//
LOCUS       DBMDSQ010000292         2484 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_9299_length_2484_cov_1.987830, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000292 DBMDSQ010000000
VERSION     DBMDSQ010000292.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 2484)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2484)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2484
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9299_length_2484_cov_1.987830"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..729
                     /locus_tag="ACZPAX_09100"
     CDS             <1..729
                     /locus_tag="ACZPAX_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HIR1114093.1"
                     /translation="SEAVSTEEAASADAAELTTVTAGKLTMSTNAAFPPYEMTTDSGD
                     FEGIDVEVAGAIAEKLGLELQVDDMDFDAALLAAQNGKSDIVMAGVTVTDERQKVMDF
                     SDTYAEGIQSIIVPEDSDIASADDLTGKAIGTQRGTTGYIYCTDDFGEDNVIAYDDGL
                     TAVQALNNGQVDAVVIDNAPAKSFVEANPGLKILDTAYAQEDYAIGVAKGNTALLDAI
                     NSALEELQADGTLQAIVDKYITAE"
     gene            959..1726
                     /locus_tag="ACZPAX_09105"
     CDS             959..1726
                     /locus_tag="ACZPAX_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HIR1114094.1"
                     /translation="MEAQFEALSELAKSGEKLNFGQDFFVKFYQAFLYSDRWQQYIKG
                     VGTTLLVTAIALVLGVLLGAIVAMIRVGHAQQKPHHRNPILGVLNAVCKVYVTVIRGT
                     PMMVQLLIMSMVIFASSRNFTMVGALALGINSGAYVSEIIRGGLMAVDPGQMEAGRSL
                     GLNYMTTMFEIIIPQAIRSILPSLGNEFIMLLKDSSLITVIGGKELLYAAQGIMNRTY
                     EPMFPLLGVAAVYLVLVMLFSALLAKFERRLAQSDRR"
     gene            1713..2429
                     /locus_tag="ACZPAX_09110"
     CDS             1713..2429
                     /locus_tag="ACZPAX_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HIR1114095.1"
                     /translation="MIDVKGLKKNYGGLQVLKGVDLTINKGDCVVLVGPSGCGKSTFL
                     RCLNRLEEPDGGHIIFNGKEVTDHDIDHVRQKMGMVFQHFNLFPHLTVKKNITLAPVR
                     LGLMKQPEADAKAMELLERIGLADKADTYPNMLSGGQKQRIAIVRALAMNPDVLLFDE
                     PTSALDPEMVGEVLELMKELARGGMTMVCVTHEMGFAREVANRIIFIDEGVVKVDKPP
                     KEFFANPENERLKAFLSKVL"
BASE COUNT          519 a          742 c          722 g          501 t
ORIGIN      
        1 tccgaggccg tttccaccga ggaggccgct tctgccgacg ctgccgaact gaccaccgtc
       61 accgccggca agctgacgat gtccaccaac gccgccttcc ccccgtatga gatgaccacc
      121 gacagcggtg actttgaggg catcgatgtt gaggtcgccg gtgctattgc tgagaagctc
      181 ggcctggagc tgcaggttga tgacatggac tttgacgccg ccctgctggc tgcccagaac
      241 ggcaagagcg acatcgtcat ggccggtgtc accgtcaccg acgagcgcca gaaagtcatg
      301 gatttctctg acacctacgc cgagggcatc cagtccatca tcgtgcccga ggattccgac
      361 attgcctctg ctgacgatct gacaggcaag gccatcggca cccagcgcgg caccaccggt
      421 tacatttact gcaccgacga cttcggtgag gacaacgtca tcgcctacga cgacggtctg
      481 accgctgtgc aggccctgaa caatggtcag gtcgacgccg tcgttatcga caacgccccg
      541 gctaagtcct tcgtggaggc taaccccggc ctgaagatcc tggacaccgc ttacgcccag
      601 gaagactatg ccatcggcgt tgccaagggc aacaccgctc tgctggacgc catcaacagc
      661 gccctggaag agctgcaggc tgacggcacc ctgcaggcca tcgtggacaa gtacatcacc
      721 gctgaataag cgcttttgta tcgcacagaa aggcacgccg ggccccggcg cgcttttctt
      781 ttatttagtg tgatttttcc cggaaatatg gtataataca aatatcgcgc tgtaggggcc
      841 gcatatatgc ggccctcaac cttcccgcag gcaagagttt gccggaaagc cgcgggccgg
      901 gcatgcccgg cccctacagg tgtgctgaat tcaaacagaa agaaaggttg tgttgcggat
      961 ggaagcacag tttgaagcgc tctctgagct ggcaaaaagc ggcgagaagc tgaacttcgg
     1021 gcaggacttc ttcgtaaaat tctaccaggc atttctctac tccgaccgct ggcagcagta
     1081 tatcaagggc gtgggcacca ccctgctggt cacggccatt gcgctggtgc taggcgtgct
     1141 gctgggggcc atcgtggcta tgatccgcgt gggccacgcc cagcagaaac cccaccaccg
     1201 caaccccatc cttggcgtgc tgaacgctgt gtgcaaggtg tatgtcacgg tcatccgcgg
     1261 cacgcctatg atggtgcagc tgctcatcat gagcatggtc atcttcgcca gcagccgcaa
     1321 ctttacgatg gtcggtgcgc tggcgctggg tatcaactcc ggcgcgtatg tctccgaaat
     1381 catccgcggc ggcctgatgg ccgttgaccc cggccagatg gaagccggcc gcagcctggg
     1441 ccttaactac atgaccacga tgtttgagat catcattccc caggccatcc gctccatcct
     1501 gccgtccctg ggcaacgagt tcatcatgct gctgaaggac tcttccctga ttaccgtcat
     1561 cggcggcaaa gagctgttgt acgcggccca gggcatcatg aaccgtacct atgagcctat
     1621 gttcccgctg ctgggcgttg cggctgtgta cctggtgctg gtcatgctgt ttagcgcatt
     1681 gctggcaaaa tttgagagga ggctggcgca aagtgatcga cgttaaagga ctgaaaaaga
     1741 actacggcgg cctgcaagtc ttgaaaggcg tagatctgac catcaacaag ggtgactgcg
     1801 ttgtattggt cggcccctcc ggctgcggca aatccacgtt cctgcgctgt ttgaaccggc
     1861 tggaagagcc ggatggcggc cacatcatct tcaacggcaa ggaagtcacc gatcacgaca
     1921 ttgaccatgt gcgccagaag atgggcatgg tgttccagca tttcaacctt ttcccccacc
     1981 tgacggtgaa gaagaacatc acgctggccc cggtgcgtct ggggctgatg aagcagcccg
     2041 aggccgacgc caaggccatg gagctgctgg agcgcatcgg tctggccgat aaggcggaca
     2101 cctaccccaa catgctgtcc ggcggccaga aacagcgcat cgccatcgtg cgtgcgctgg
     2161 ccatgaaccc cgacgtgctg ctgtttgacg agccgacctc cgcactggat cccgagatgg
     2221 tcggcgaggt tttggaactg atgaaggagc ttgcccgcgg cggcatgacg atggtctgcg
     2281 tcacccacga aatgggcttt gcccgcgagg tggcgaaccg aattatcttt atcgatgagg
     2341 gcgtcgtcaa ggtcgacaaa cccccgaagg agttcttcgc caaccccgaa aacgagcgct
     2401 tgaaagcatt cttgtcgaag gtgctgtaat tgcatagaaa aagcggtctg ccgatagatt
     2461 ttcggcaggc cgcttttcgc tttt
//
LOCUS       DBMDSQ010000293         2477 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_9332_length_2477_cov_1.406145, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000293 DBMDSQ010000000
VERSION     DBMDSQ010000293.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 2477)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2477)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2477
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9332_length_2477_cov_1.406145"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..555
                     /gene="rsmD"
                     /locus_tag="ACZPAX_09115"
     CDS             <1..555
                     /gene="rsmD"
                     /locus_tag="ACZPAX_09115"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006353342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HIR1114096.1"
                     /translation="GTMRVIAGTARGKNLQALPGEDITRPTINRVKEAMFSSVQFLVP
                     GARVLDLFAGSGQLGIEALSRGAKSCLFVDHSAEALAIVRANCKTAGVDRQSDIRQGE
                     AESFLANIRGPFDLVLLDPPFHHDTVAAVLPLLAAKMAPGGVVLCETEREADLPEQAG
                     SLTLVKQYNYGKIKVSRYERGEEA"
     gene            552..1022
                     /locus_tag="ACZPAX_09120"
     CDS             552..1022
                     /locus_tag="ACZPAX_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HrpE/YscL family type III secretion apparatus
                     protein"
                     /protein_id="HIR1114097.1"
                     /translation="MNVEELLELMEETLEAGTAVPFAASKRVVDVDRMRDIIDEVRNN
                     LPDEVRESKKIVNDREQIMKNARIESENIIQQAEERARTLTSEQEIVKRSQQRAVEIL
                     TAAQTQAKELSRNATVYCEGILKNSEEVLARSVADIKNTRMNLRNAARPNRGQK"
     gene            1435..1851
                     /gene="mraZ"
                     /locus_tag="ACZPAX_09125"
     CDS             1435..1851
                     /gene="mraZ"
                     /locus_tag="ACZPAX_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HIR1114098.1"
                     /translation="MLVGQYDYAIDAKGRLNFPARFRDAMGETFIVTRWLDHCLAAFP
                     AEEFEKVAAKIEEKGLVKGRKVSRMLYASAVEVTPDKQGRIQLPAKLREYAGLDHDVT
                     IIGSRSFAEIWNTEAWNASQETSDEDFTAEMENLDF"
     gene            1870..>2477
                     /gene="rsmH"
                     /locus_tag="ACZPAX_09130"
     CDS             1870..>2477
                     /gene="rsmH"
                     /locus_tag="ACZPAX_09130"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000154 - rRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HIR1114099.1"
                     /translation="MEFSHIPVLLQPCLDGLNIDPAGIYLDGTAGGAGHSREIAKRLT
                     TGRLISLDQDPDAVAVATERLKGLPAQVVQVNFREAARVLAELGIPAVNGALLDLGVS
                     SHQLDDAARGFSYHADAPLDMRMSQQGPTAADLVNTLDREELTRILRDYGEEPYAWQI
                     AGKIIAARETRPITTTMQLADIVAAAVPPAERRKAKNPARRT"
BASE COUNT          568 a          709 c          740 g          460 t
ORIGIN      
        1 ggcaccatgc gcgtcattgc cggaacagcc cgcggtaaaa acctgcaggc cctgccgggc
       61 gaggatatca cccgcccgac catcaaccgc gtgaaggagg ccatgttctc cagtgtgcag
      121 tttcttgtgc cgggagcacg cgtgctggat ctgtttgcag gcagcggtca gctgggcatc
      181 gaggccctgt cacgcggggc gaaaagctgc ctctttgtcg accactctgc cgaagcattg
      241 gccatcgtaa gggctaactg caagacggcg ggcgttgacc gccagagtga tatccgccag
      301 ggcgaggccg agagcttcct tgccaacatc cgcgggccgt ttgacctggt cctgctggat
      361 ccgccgttcc accatgacac ggtggcggcg gtgctgccgc tgctggccgc caagatggcc
      421 ccgggcggtg tggtcctctg cgagacggag cgtgaggccg acctgcctga gcaggcaggc
      481 agcctgacac tggtaaaaca atacaactac ggcaagatca aggtcagccg ctatgagaga
      541 ggggaagagg catgaacgtt gaagaattgc tagaactgat ggaagaaaca ctggaagcgg
      601 gtacggctgt gccgtttgcc gcctccaagc gcgtggtcga cgtggatcgt atgcgcgata
      661 tcattgatga ggtgcgcaac aatctccccg atgaagtgcg cgagagcaag aaaatcgtga
      721 atgaccgcga gcaaattatg aaaaatgccc gcatcgagtc ggaaaatatc attcagcagg
      781 ccgaggagcg cgcccgcacc ctgaccagtg agcaggagat cgtcaagcgc agccagcagc
      841 gtgcagtgga aattctgacc gccgcccaga cccaggccaa ggagctgagc cgcaacgcta
      901 cggtctactg cgagggcatc ctgaaaaact ctgaggaagt gctggctcgc agcgtggcgg
      961 atatcaagaa tacccggatg aacctgcgca atgccgcccg ccccaaccgt gggcagaagt
     1021 aaggaaggcc ttgaaaaacg ttgttatcca cggcacattt ccctaaaaat ctttataagc
     1081 tctaaaaata tcgtcgcacc gtgtaatacg gtgcggcgat atttttgtct gcggcaaaaa
     1141 ttttgccgcc gtgctgtttt gatgcaaacc gctgtccctg gggtaggggc ctttgtacaa
     1201 agtgtgaaaa atccattcgc ggcgcagttt tttccagttc gcccttgctt tttgccgggg
     1261 aaaatggtac tataatttta ccacgagtgg gtaattgtgg tgagcttaac aacaaaagtg
     1321 gtgaatagtg ggtagttttc cacgttctcc acagagtttt ccacaaagag aacgctgcga
     1381 cgtaaaaaat cttgctgcaa gtttgtacag tttgacgaag ggaggggaac acagatgctc
     1441 gttggtcagt atgattatgc catcgatgcc aagggccgtc tgaatttccc cgcccgtttc
     1501 cgcgacgcga tgggggaaac cttcattgtg acccgctggc ttgaccattg cctggccgct
     1561 ttcccggcag aagagttcga aaaggttgcc gccaaaatcg aggaaaaggg cctcgtcaag
     1621 ggccgcaagg tcagccgtat gctgtacgcc tctgcggtgg aggttacccc ggacaagcaa
     1681 ggccgcatcc agctccccgc caaactgcgg gagtacgcag gccttgacca cgatgtcacc
     1741 attatcggca gccgcagctt tgccgagatt tggaacaccg aggcatggaa cgccagccag
     1801 gaaaccagcg atgaggactt caccgctgag atggaaaacc ttgactttta ataaacagga
     1861 cgataaaaca tggaattttc acatatcccc gtactgctgc agccctgcct ggatgggctg
     1921 aacatcgacc ccgccggcat ctacctggat ggcacggccg gcggcgcggg ccacagcaga
     1981 gaaattgcaa aacgactgac cacaggccgc ctgatttcac tcgaccagga cccggatgcg
     2041 gtggcagtag ccaccgaaag gctcaagggt ctcccggcgc aggtggtgca ggtcaacttc
     2101 cgcgaggcag cgcgagtcct cgcggagctg ggcatcccgg cggtgaacgg agccctgctt
     2161 gatctcggcg tgtccagcca ccagctggac gatgcagcgc gcggtttttc ctaccatgcc
     2221 gacgcgccgc tggatatgcg catgagccag cagggcccca ccgccgcgga cctggtcaac
     2281 actctggatc gtgaggaact gacccgcatc ctgcgcgact atggcgagga accctacgcc
     2341 tggcaaatcg cgggcaaaat catcgccgcg cgggaaacgc ggcccatcac caccaccatg
     2401 cagttggcgg atattgtggc cgccgcagtg cccccggctg agcgccgcaa ggccaagaac
     2461 ccggcgcgcc gcacttt
//
LOCUS       DBMDSQ010000294         2470 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_9364_length_2470_cov_1.604052, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000294 DBMDSQ010000000
VERSION     DBMDSQ010000294.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 2470)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2470)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2470
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9364_length_2470_cov_1.604052"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            397..1725
                     /locus_tag="ACZPAX_09135"
     CDS             397..1725
                     /locus_tag="ACZPAX_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005940287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015851 - nucleobase transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-xanthine permease family protein"
                     /protein_id="HIR1114100.1"
                     /translation="MAEKIDYSQGIYDAKQLGTPRLLILGAQHMFAMFGATVLVPLLT
                     GLSVSTTLLCAGLGTLLFHFLCKGKVPAFLGSSFAYLGGFTIVTNGGANPENLPYACA
                     AVALSGLVYVLFSALISAFGIRKMMKFFPPVVTGPIIIAIGLILAPSAINNCQSNWLL
                     AFVALATVIVCNIWGKGMIKILPILIGVLVSYAVALVTGAIDFQAIGAAAWFGIPLHK
                     DSMGLFAIDGSETFISAVFTIVPIALATMMEHIGDIAAISATTGKNYIRDPGLNKTLL
                     GDGLATAMAGLLGGPANTTYGENTGVLALTKIYDPLVIRIAAVFAMILSFCPKFEAVI
                     NTIPSGIVGGISFVLYGMISAIGVRNVVENKVDFTNSRNLIIAAVILVSALGFNSVGG
                     ISFGLGNVTINLSGLAIAAIAGILLNAILPGNDYEFDEGSNEPESASLRV"
     gene            1844..2197
                     /locus_tag="ACZPAX_09140"
     CDS             1844..2197
                     /locus_tag="ACZPAX_09140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1114101.1"
                     /translation="MTASDFTNLHLQYKSAQADGEVPAVIEHDFPGGRMVDHYFVTPS
                     PAFWADEGVQSLDGVSGILFLQQPSGAPWKILVHEQSMIKEVVFDFPEEEFRKMLADN
                     GVTLPGEPGFAPITD"
BASE COUNT          481 a          770 c          660 g          559 t
ORIGIN      
        1 gttctgcgaa aggtttcctg cgatttcatt caccgcttct gcctttgtct gggtctgaca
       61 atagaacaac ttttgttcaa gaatgcctcg aattttttgc tcgccatctg caaaggcgag
      121 agccatctca tatctgatat aataacacac tttccgacaa aaagatagta gggggccggg
      181 catacccggc ccgcgcgtgt aaggcggacg gccatttgtg ggaaagttga gggccgggcg
      241 tgcccggcca ctacatgacg acaaaatttt cctaacatct tgcactttcc cgctatttct
      301 gctatcctta tagagataag caagggggct gtggtttttg ggtaaaaaac gttcagtctc
      361 ccttttttgc ggccattgtg aggaggaatt tcatttatgg cagagaaaat cgattactcg
      421 cagggcatct atgatgccaa gcagctgggc acgcccaggc tgctgatcct cggcgcacag
      481 catatgttcg ccatgttcgg cgctaccgtg ctggtgccgc tgctgaccgg cctgagcgtc
      541 agcaccaccc tgctctgcgc aggtctgggc acgctgctgt tccacttcct gtgcaagggc
      601 aaggtccccg cgttcctggg ttccagcttc gcgtatctgg gcggctttac catcgtgacc
      661 aacggcggtg ccaacccgga gaaccttccc tacgcctgcg cggccgtggc cctgtccggc
      721 ctggtctatg tgctgttcag cgcactgatc tccgcgttcg gcatccgcaa gatgatgaag
      781 tttttccccc cggtcgtcac cggccccatc atcatcgcca tcggtctgat cctggccccc
      841 agcgccatca acaactgcca gagcaactgg ctgctggcct tcgtcgcgct ggcaaccgtc
      901 atcgtctgca acatctgggg caagggcatg atcaagatcc tgcccatcct catcggcgtg
      961 ctggtctcct acgcggtcgc gctcgtgacc ggtgctatcg acttccaggc catcggtgcg
     1021 gcggcctggt tcggtatccc cctgcataag gattccatgg gtctgttcgc catcgacggc
     1081 agcgagacct tcatcagcgc cgtgttcacc atcgtgccca tcgcgctggc aaccatgatg
     1141 gagcatatcg gcgatattgc cgctatctcc gccaccaccg gcaagaacta catccgtgac
     1201 ccgggcctga acaagaccct gctgggcgac ggccttgcca ccgccatggc cggcctgctg
     1261 ggcggccccg ccaacaccac ctacggcgag aacaccggcg tgctggccct gaccaagatc
     1321 tacgaccctc tcgtcatccg cattgcagct gtgtttgcca tgattctgag cttctgcccg
     1381 aagtttgagg ccgtcatcaa cacgatcccc tccggcatcg tcggcggcat cagctttgtg
     1441 ctgtacggta tgatctccgc catcggcgtg cgcaatgtcg ttgagaataa ggttgacttc
     1501 accaactccc gcaacctgat tatcgccgcc gttattctgg tcagcgcgct gggcttcaac
     1561 tcggtgggcg gcatcagctt tggtctgggc aatgtcacca tcaacctgtc cggcctggcc
     1621 atcgccgcca tcgccggtat cctgctgaac gccatcctgc ccggcaacga ctacgagttc
     1681 gacgagggca gcaacgagcc tgagtccgcc agcctgcgcg tatgataaac gactgccagg
     1741 cggcggggtg aggacacccc cctacgacca gcccaaatgt gcggcgtagg gcggtatacc
     1801 cacataccgc cgcaggcagc actttacgga aaggaatgct tctatgaccg cttccgactt
     1861 caccaacctg cacctgcaat acaaatccgc ccaggctgac ggcgaggtcc ctgcggtcat
     1921 tgaacatgac ttcccgggcg ggcggatggt ggaccactac tttgtcaccc cctcccctgc
     1981 tttctgggct gacgaaggcg tgcaaagtct cgacggcgtg tccggcatcc tgtttttgca
     2041 gcagcccagc ggtgcgccgt ggaagatact cgtccatgag cagagcatga ttaaagaagt
     2101 tgtctttgac tttcccgagg aagagttccg aaaaatgctg gccgacaacg gcgtgaccct
     2161 gcccggcgag ccgggctttg cccccatcac cgactaaggg caacaccgca caattcccgc
     2221 ctgacggctt aagcaccgct ccagcggctg cggtacggca tctgcgttgc caaaatgctc
     2281 gataatacac aaagtattat ctgcgctttt ggcttagcag ctgccgcacc tcgctcgccg
     2341 tatcggcact tagaattatg cggtattgcc ctgagatttc ccccggatgt gagaattttc
     2401 acatccgggg gattcttttg tgtttttcta ttgacaaagt gtatattagg atgtatcata
     2461 tgaacaaacg
//
LOCUS       DBMDSQ010000295         2469 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_9365_length_2469_cov_1.835726, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000295 DBMDSQ010000000
VERSION     DBMDSQ010000295.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 2469)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2469)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2469
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9365_length_2469_cov_1.835726"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..459
                     /locus_tag="ACZPAX_09145"
     CDS             <1..459
                     /locus_tag="ACZPAX_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006874260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HIR1114102.1"
                     /translation="NMTVLDNCTAGLRYVLHQDKETAQKTALENLEKVGMAPYIHAKP
                     RQLSGGQKQRVAIARALAMQPEVLLFDEPTSALDPQMVGEVLAVMRQLAKEGLTMIVV
                     THEMAFARDVSSHVVYMADGVICEEGTPADIFEHPQNPKTVEFLSRFRQA"
     gene            complement(836..>2469)
                     /locus_tag="ACZPAX_09150"
     CDS             complement(836..>2469)
                     /locus_tag="ACZPAX_09150"
                     /inference="COORDINATES: protein motif:HMM:NF024117.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HIR1114103.1"
                     /translation="IPLAAVLLSPLFPYTADDLVALRCACPEGSLYGAVLHGGQAQFA
                     VFIEALTEYRRLARTLPVDELIEELLARTGYLAAVGALPDGPRCREDLLSFTAWAAGA
                     GRAGLPALIRAMDAAAANGGLSQSVGGQTRPGCVSIMTVHRSKGLEFPVVFVADTDHK
                     FNQSDAIRPVLTHSRLGVGVMLRAPRAAKRFKTLPYAALAQAIRTETLSEEMRVLYVA
                     LTRAQDALIITVPLKKTASALKTPALCAAAEATGSESMRSMGSWAGWILTAAMLHPDS
                     DALWGYTSFLPHHRPTEVPLTIRLVALPENAEQPPAPRAPQPDPATVEMLKASFAWHS
                     PRAALEKVPAKVSVSAVAHAARPVALERPAFLQKTGMTGAERGTAIHAFMQSVPFDGP
                     APDLDTEVKRQTDLQLLDAALADKLDLDAVRPFFESAVWRRIRHAKQILREEPFITAL
                     PAAEVTPAAQQGSAAEAEVLVQGIADLVLVFDEHAEILDYKTDRSRDAQYYIDEYAAQ
                     LRLYRRAFAQRLSVPVTKLTIYSFAIGDEIDVPLA"
BASE COUNT          432 a          766 c          825 g          446 t
ORIGIN      
        1 aacatgactg tgctggacaa ctgcacggca ggcctgcgct acgtgctgca ccaggataaa
       61 gaaactgcgc agaagaccgc gctggaaaac ctggaaaagg tcgggatggc cccctatatc
      121 cacgccaagc cccgccagct ttccggcggc cagaagcagc gcgtcgcgat tgcccgcgcc
      181 ctggccatgc agcccgaagt gctgctgttc gacgagccga cgtctgcgct tgacccccag
      241 atggtaggtg aggtcctggc cgttatgcgc cagttggcga aggagggtct gacgatgatc
      301 gtcgtcaccc acgagatggc ctttgcccgg gatgtttcga gccatgtggt ctacatggcc
      361 gacggcgtca tctgtgagga aggcaccccc gccgatatct tcgagcaccc gcagaacccc
      421 aagaccgttg agtttctgag ccggttccgg caagcgtaaa ttggtataaa tactaaaggt
      481 cgtgctgacg atggattgtc ggcacgacct tttttgtttg tgatattggg atacggcccg
      541 gaagggagga atcccgcccc tacggatggc ggaaagctca tgacgaaaga tgtagggggc
      601 ggcgtcctcg acgccccgcg ggtcagcagg tatttccggg tgggacgtag ggcggcctgc
      661 cctcaggccg ccgcgcccgt ttgcagttgt ggaaaagttt ccgtattgcc gttgtagggg
      721 gcggcgtcct cgacgtcccg cggaggggtc aagacccctc cctacgatgc aaaagataaa
      781 ggggcagtaa ttgcaaggtc gcgggccggg catgcccggc ccctacagcc tgtttttacg
      841 ccagcggcac gtcgatctcg tcgccgatgg caaagctgta aatcgtcagc ttcgtcacgg
      901 ggacgctcag ccgctgggca aacgcacggc ggtagagccg cagctgggcg gcgtactcgt
      961 cgatataata ctgcgcgtcg cggctgcggt cggtcttgta gtcgagaatt tccgcgtgct
     1021 catcaaacac cagtaccaaa tcggcgatgc cttgcacgag cacttccgct tcggcggcac
     1081 tcccctgctg tgcggcgggg gtgacttcgg cggcgggcag ggccgtgatg aacggttcct
     1141 cccgcaaaat ttgtttcgca tggcggatgc gccgccagac tgcactctca aagaagggcc
     1201 gcacggcgtc caggtcgagc ttgtctgcca gcgcggcatc caacagctgc aaatccgtct
     1261 ggcgtttgac ctcggtgtcc aggtcggggg ctgggccgtc gaagggtaca ctctgcataa
     1321 aggcgtggat ggcggtaccg cgctcggccc cggtcatgcc ggttttctgc aaaaaggccg
     1381 ggcgttccaa tgccaccggg cgggccgcat gggccacagc gctgacgctg accttggcgg
     1441 gtactttttc cagcgcggcg cgggggctgt gccaggcaaa gctggctttc agcatttcga
     1501 ctgtggccgg gtcgggctgg ggtgcgcggg gggcgggcgg ctgctcggca ttttccggca
     1561 gcgccaccag acgtatcgtc agcggcacct ccgttgggcg gtggtggggc agaaagctcg
     1621 tgtaccccca cagcgcgtcg ctgtccgggt gcagcatggc ggcagtcagt atccacccgg
     1681 cccagctgcc catactgcgc atcgactcac tgccggtggc ctcggctgcc gcgcagaggg
     1741 ccggtgtttt cagcgcactg gcggtctttt tcagcggcac ggtgatgatg agcgcatcct
     1801 gggcacgggt cagcgccaca tacagtacgc gcatttcctc gctcaatgtc tcggtgcgga
     1861 tagcctgggc cagcgccgca tagggcagcg ttttgaaccg cttggccgcc cggggcgcac
     1921 gcagcataac gcccacgccc agacggctgt gggtcagcac cgggcggatg gcatcgctct
     1981 ggttaaattt gtggtcggtg tccgccacaa aaacgaccgg gaattccaga cccttggagc
     2041 gatgtactgt catgatggag acgcaccccg gccgggtctg gccgcccacg ctttggctca
     2101 ggccgccgtt ggctgctgcg gcgtccatgg cgcggatcag cgccggcagg cccgcgcggc
     2161 ctgcaccggc ggcccaagcc gtaaagctca acaggtcctc acggcagcgc gggccgtcgg
     2221 gcagtgcgcc cacggcggcc aggtagccgg tgcgggccag cagttcctcg atcagttcat
     2281 ccaccggcag tgtgcgggcc aggcggcggt actcggtcag tgcctcgata aagacggcaa
     2341 attgtgcctg cccgccgtgc agcaccgcac cgtacaggct gccctccggg caggcacagc
     2401 gcagtgccac taaatcatcc gccgtgtacg ggaacaaagg gctgagcagc accgccgcca
     2461 gcgggatat
//
LOCUS       DBMDSQ010000296         2465 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_9392_length_2465_cov_1.504230, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000296 DBMDSQ010000000
VERSION     DBMDSQ010000296.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 2465)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2465)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2465
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9392_length_2465_cov_1.504230"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            85..1605
                     /gene="asnB"
                     /locus_tag="ACZPAX_09155"
     CDS             85..1605
                     /gene="asnB"
                     /locus_tag="ACZPAX_09155"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0070981 - L-asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HIR1114104.1"
                     /translation="MSREWGADCVERCNGIFAFAVWRVNAGTLFLARDRCGVKPLFYT
                     QAEDSLIFGSELKTLLAHPAVPPRIDANGLAEVLLLGPGRTPGCGVFQNVRELLPGQC
                     ATYDADTARLTLHTYWQLTDHDHPDDFAATTRKVRDLVTDAIERQLVSDVPVATFLSG
                     GLDSSLISAIADTHFAAQGKQLQTFSVGYRDNKKYFHATKFQPGADAPYIRKMNDFLH
                     ARHHWVTLDTPELVPALYAAVDARDLPGMADVDASLLVFCQHIKPHATVALSGECADE
                     IFGGYPWYRDPAVREKYGFPWAQSTAYRASFLKPGVLRGIDPASFVDARYQQTLAETS
                     IRPGLPATERRMRQMINLNFKWFMQTLLDRKDRMSMYSGLEVRVPFCDYRIAEYLYSV
                     PWEFKDWGGQEKGLLREAMRGVLPDEVLWRRKSPYPKTWNPSYLAAVIAELRGILVDP
                     AAPILRIIKADALETLLASGTDNPIPWYGQLMTTPQTIAWFVQLNYWLEKYKVELV"
     gene            1788..>2465
                     /gene="guaA"
                     /locus_tag="ACZPAX_09160"
     CDS             1788..>2465
                     /gene="guaA"
                     /locus_tag="ACZPAX_09160"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008374035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HIR1114105.1"
                     /translation="MQQETVIVLDFGGQYNQLIARRVRECNVYCEIYSYRTDLAKIKA
                     KNPKGIIFTGGPNSAYLPDSPTIDPEIYNWGVPILGICYGSQLMMHMLGGKVCKAPER
                     EYGKTLVDLDTASPLFDTLPAQNVCWMSHNDYIEQAAPGFEIVAHTPNCPVAAAQDKA
                     RGLYAVQFHPEVLHTENGTKILHNFVYNVCHCTGDWKMDSFVENSVKALREKIGDGKV
                     LCALSGGV"
BASE COUNT          524 a          737 c          693 g          511 t
ORIGIN      
        1 aacctttgat agccgtttcg gaaacctacg tggacggaaa acaagtagac ggacaacatt
       61 actattacga agggggcata caacatctca cgggaatggg gcgcggattg cgtggaacgg
      121 tgcaacggta tttttgcctt cgcagtctgg cgggtgaacg caggtacact ctttctggcc
      181 cgtgaccgct gcggcgtcaa gccactgttc tatacccagg ccgaggacag cctgattttt
      241 ggaagcgaac tgaaaaccct gctggcccac ccggcagtac cgccgcgcat cgacgccaac
      301 ggtttggctg aagtactgct gctaggccct gggcgcacgc cgggctgcgg tgtttttcaa
      361 aacgtgcggg aactgcttcc cggccagtgc gcaacttacg atgccgacac cgcccgcctg
      421 acgctgcaca cctactggca gctgaccgac cacgaccacc ccgatgattt tgcggccacg
      481 acgcgaaaag tccgtgacct ggtgacggat gccattgagc gccagcttgt ctctgacgta
      541 cccgtggcga cattcctctc gggcgggctg gattccagcc tgatttccgc cattgccgac
      601 acccattttg cggcgcaggg aaagcagctg caaacttttt cggttggcta ccgcgacaat
      661 aaaaagtact ttcatgcgac aaaattccag cccggtgcgg atgcacccta catccgcaag
      721 atgaacgatt ttctccacgc ccgccaccac tgggtcacgc tggacacccc ggagcttgtc
      781 cctgcgctgt atgcggcggt ggatgcccgc gacctgcccg gcatggccga tgtggatgcg
      841 tcactgcttg tgttttgcca gcacatcaag ccccatgcca ctgtggccct gtcgggggaa
      901 tgcgccgatg agattttcgg cggctatccg tggtatcgtg accccgccgt gcgggaaaag
      961 tacggctttc cctgggcgca gtccacagcc taccgcgcgt cgttcctcaa gcccggcgtg
     1021 ctgcgcggca ttgacccggc ttcctttgtg gatgcacgct accagcagac cctggcagaa
     1081 acatccatcc gccccgggct gcccgcaacg gaacgccgta tgcgccagat gatcaacttg
     1141 aatttcaagt ggttcatgca gacgctgctc gaccgcaagg atcgcatgag tatgtacagc
     1201 ggcctggaag tgcgcgtgcc attctgcgat taccgtattg cggagtatct ctactcggtg
     1261 ccgtgggagt tcaaagactg gggcgggcag gagaagggct tgctgcgcga agccatgcgc
     1321 ggagtgctgc cggatgaagt actgtggcgg cgcaaaagcc cctaccccaa aacttggaac
     1381 cccagctacc tggcggcggt tatagccgaa ctgcgaggta ttctggtcga ccccgccgca
     1441 ccaattttgc gcattatcaa ggccgacgca ctggaaaccc tgctggcgtc gggcacggat
     1501 aaccccatcc catggtacgg tcagcttatg accactccac agaccatcgc atggtttgtg
     1561 caactgaatt attggttgga gaaatataag gtggaactgg tatgaacttg cggcggggtg
     1621 ggggcacccc gccctacata ttttgcttgt tgggaaaggt aggacggtat gccctcatgc
     1681 cgccgcgggc cgccacccgc aaatattttt gtatcccatc tgttcaaacc cgcccaaacg
     1741 tgttataatc atataattag gaatacaaaa gagaagggag acccctcatg cagcaggaaa
     1801 ctgtcatcgt tctggatttc ggcggtcagt ataatcagct gattgcccgc cgtgtccgcg
     1861 aatgcaacgt atattgcgag atctattctt accgtaccga ccttgccaaa atcaaggcca
     1921 agaacccgaa aggcattatc tttaccggcg gtcccaacag cgcttacctg ccggattcgc
     1981 ccactatcga ccccgaaatt tacaactggg gcgtgcccat cctcggcatc tgctacggca
     2041 gccagttgat gatgcacatg ctgggcggca aggtctgcaa agcgcctgag cgcgagtacg
     2101 gtaaaacgct ggttgacctg gacaccgcca gcccgctgtt tgatacgctc cccgctcaga
     2161 acgtctgctg gatgagccac aatgactaca tcgaacaggc tgcccccggc tttgagatcg
     2221 tcgcgcatac ccccaactgc ccggtggctg ccgcgcagga taaggcccgc ggcctgtatg
     2281 cggtgcagtt ccatcccgaa gtactgcaca ccgagaacgg cacgaaaatt ctgcataact
     2341 tcgtctataa tgtgtgtcac tgcactggtg actggaagat ggattctttc gtcgagaact
     2401 ccgtcaaggc cctgcgcgag aagatcggcg acggcaaagt gctgtgtgcc ctgtccggcg
     2461 gcgtt
//
LOCUS       DBMDSQ010000297         2458 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_9417_length_2458_cov_1.917692, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000297 DBMDSQ010000000
VERSION     DBMDSQ010000297.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 2458)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2458)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2458
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9417_length_2458_cov_1.917692"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..592
                     /locus_tag="ACZPAX_09165"
     CDS             <1..592
                     /locus_tag="ACZPAX_09165"
                     /inference="COORDINATES: protein motif:HMM:NF024097.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SpoIIIAH-like family protein"
                     /protein_id="HIR1114106.1"
                     /translation="ALGAAVYLNWSFSREAPSSLVVSEDIAADAVETSAAVSEILTDP
                     LAVETAADAEPAAEELGDKNYGEAQLVSVNQDSGTEFFESARLTRSKARDEALDTLKK
                     SLKDAKLSDAEKDQLTTELSARISNITLETKLETLIKSKGFADCVVNLEGSKANVTVM
                     TENDALTAEEVTRIRDALLNQCKGLTAQDITIVEVK"
     gene            691..1053
                     /locus_tag="ACZPAX_09170"
     CDS             691..1053
                     /locus_tag="ACZPAX_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005942042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HIR1114107.1"
                     /translation="MDVHNTIGGSLQISTEVIAKIARLAALEVDGVSDVATGTSQNVR
                     SLLGRTGLQKPVTVVMEDGIATVTVHITVVYGSKVMPLCEKVQENVKQAIQNMTGITV
                     ARVDVLVVGLTDADETEK"
     gene            1085..1498
                     /gene="nusB"
                     /locus_tag="ACZPAX_09175"
     CDS             1085..1498
                     /gene="nusB"
                     /locus_tag="ACZPAX_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HIR1114108.1"
                     /translation="MEKKLTRRESREMAFLTAFAATFEPEEPTVPAEDHAAPDAFANQ
                     LLAAMNDHAAELDEIITAHLKGWTLPRVPRVSLVALRLALAEMLYGEEQKPGVAINEA
                     VELVKKYGADDDYQFVNGLLGSVARERGEAPESQC"
     gene            1492..2439
                     /locus_tag="ACZPAX_09180"
     CDS             1492..2439
                     /locus_tag="ACZPAX_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HIR1114109.1"
                     /translation="MLTLGIDTSNYATSLAVFDTDAGEVVCDCKKFLPVKEGQLGLRQ
                     SDALFHHTTALPEMLAELSGKADLTKVAAVGVSAKPRPVEGSYMPCFLAGVSAATAFA
                     LAKGLPMVKLTHQQGHIAAALYATGDLTLFEREALVFHVSGGTTDLLLCHEAESITPL
                     GTSSDLYAGQAVDRLGVKLGFPFPAGVYVSDQAALCTEKVRPKVSVKGLTCSLSGLEN
                     QCAKLLAEGHDAPYVCKYCLLCVGETLVRMANNALAECPGLPVVFAGGVMSSDLIRTY
                     VTNRVPNAHFVPGKFASDNAIGISILAARECGAWPTTSM"
BASE COUNT          533 a          722 c          748 g          455 t
ORIGIN      
        1 ggcgctggga gccgccgtgt atctgaactg gtccttctcc cgcgaggccc cgtcgagcct
       61 ggtcgtctcg gaggatattg ccgccgatgc cgtggagaca tccgccgctg tatccgaaat
      121 cctgaccgac ccgctggctg tagagactgc agcagatgcc gaacccgccg cagaggaact
      181 gggcgataaa aactatggcg aagcccagct ggtcagtgtg aaccaggatt ccggcacaga
      241 gttttttgag tccgcccgct taacgcgcag caaggcccgg gacgaagcgc tggacacctt
      301 gaaaaagtcc ctgaaggacg ccaaactcag cgacgccgaa aaagaccagc tgaccactga
      361 gctcagcgcc cgcatcagca atattacgct ggaaaccaag cttgaaacgc tcatcaagtc
      421 caagggcttc gccgactgtg ttgtcaacct ggagggcagc aaggccaacg tcaccgtcat
      481 gaccgagaac gatgccctga cggcggagga ggtcacccgc atccgcgacg cgctgctgaa
      541 ccagtgcaag gggctgaccg cgcaggatat caccatcgtg gaagtaaaat aactgaacat
      601 tctgttgcaa acgccgccgt tttgtggtac actgaatcat attagtagaa ctatggccaa
      661 aaggccgttt gcaacgggag ggtacacatt atggatgtac acaataccat cggcggcagc
      721 ttacagatct cgaccgaagt tatcgcaaaa atcgcgcgtc tggccgcgct ggaggttgac
      781 ggtgtttctg atgtagccac cggcaccagc cagaacgtcc gttcgctgct gggccgtacc
      841 ggcctgcaga agcccgtcac cgtcgttatg gaggatggca ttgccaccgt cacggtacac
      901 atcaccgttg tgtacggcag taaggtcatg cctctgtgcg agaaagtgca ggagaacgtc
      961 aagcaggcca tccagaacat gaccggcatc accgtcgccc gcgtcgatgt gctggtcgtt
     1021 ggcctgacag acgccgacga gactgagaaa taagagagaa tcaggaaaga caggaatttt
     1081 gagtatggag aaaaagctga cccgccgcga gtcccgcgag atggcctttc tgacggcatt
     1141 tgccgccacg tttgaacccg aagagcccac cgtgcccgct gaggaccacg ccgcccccga
     1201 cgcctttgcc aaccagctgc tggccgccat gaacgaccac gccgccgagt tggatgagat
     1261 catcaccgcc cacctgaagg gctggacgct gccccgcgtg ccccgcgtga gcctggttgc
     1321 cctgcgcctt gccctggcgg aaatgctgta cggcgaggag cagaagcccg gcgtcgccat
     1381 caatgaggct gtggagctgg tcaaaaaata cggtgctgac gacgattatc agtttgtcaa
     1441 cggcctgctg ggttccgtcg cgcgggagcg cggagaggcc ccggaatccc aatgctgacg
     1501 ctgggcatcg acaccagcaa ttacgcaacc tctctggcag tgtttgacac agacgccgga
     1561 gaggttgttt gcgattgcaa aaagtttttg cctgtcaaag aaggtcaact gggcctgcgc
     1621 cagagtgacg cgctgttcca ccacacgacg gccctgccgg agatgctggc tgagctttcc
     1681 ggcaaggccg acctgaccaa ggttgccgcc gtcggcgttt cagccaagcc acgcccggtg
     1741 gaagggtcgt atatgccgtg ctttctggct ggtgtcagcg ccgccacggc gtttgcactg
     1801 gccaaagggc tgccgatggt caagctgacc caccagcagg ggcatattgc cgccgcactg
     1861 tacgccaccg gcgatcttac gctgtttgag cgggaggcgc tggtgtttca cgtatccggc
     1921 ggcacgactg acctgctttt gtgccatgag gccgagagca tcacaccgct gggcaccagc
     1981 agcgatttat atgccgggca ggccgtggac cggctgggcg tcaaacttgg ctttccgttc
     2041 ccggcgggcg tctatgtcag cgatcaggcc gctttatgca ccgagaaagt gcgccccaag
     2101 gtcagcgtaa aagggctgac gtgcagttta tcaggtcttg aaaaccagtg cgccaagctg
     2161 ctggcggaag ggcacgacgc accctacgtc tgcaaatact gcctgctctg cgtgggtgag
     2221 acactggtgc ggatggcgaa caacgcttta gcagagtgcc cgggcctgcc ggtcgtgttt
     2281 gcgggcggcg tgatgagcag tgatttaata cgcacctatg tgacgaaccg cgtgccgaac
     2341 gcgcattttg tgccgggcaa atttgccagt gacaacgcca tcggcatctc gatcttagcg
     2401 gcaagggagt gtggcgcatg gccgactaca tcaatgtaac cagcctgaac cggctggc
//
LOCUS       DBMDSQ010000298         2454 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_9435_length_2454_cov_1.456014, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000298 DBMDSQ010000000
VERSION     DBMDSQ010000298.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 2454)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2454)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2454
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9435_length_2454_cov_1.456014"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1224)
                     /locus_tag="ACZPAX_09185"
     CDS             complement(<1..1224)
                     /locus_tag="ACZPAX_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015565126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HIR1114110.1"
                     /translation="MANLNPLLLVGGVIGTVTAILIFAYALVKDKKETMGFERTMKDG
                     EILRRLAGYAKPYWAKFVVVLFLMLFSIAYDIISPLIVGALEELVAGEFALPRLFAGV
                     AVYAGVLVFSMASTYLQAVILQRVGQRIISDLREDLFTHIESLAHEQLNEIPVGKLVT
                     RVTNDTNAISMMFTNLLVTLAKNIFVILGILIAMLSLNYELTLMVLCFVPFIVIFTVI
                     FRKFSRRAYRKVKDATTDINTYLSENLSGIKVTQIFGREDEKMAEFREKSQRLAKANQ
                     EQIFVFGVFRPLVYMLYICSILCLFYLGGMGHLTGATFLGQTISGGTIVTFYMYISKF
                     FTPIQNLAEQFNWLQSALASSEKVFSIMDIQPKMVDAPDTVELDEVKGEIEFRDVWFS
                     YIPGEWVLQGVSFHVS"
     gene            complement(1217..>2454)
                     /locus_tag="ACZPAX_09190"
     CDS             complement(1217..>2454)
                     /locus_tag="ACZPAX_09190"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1114111.1"
                     /translation="GTVMSQVMTKRWEERQQAFSDLSDFAQENFSGIAVIKAFVKELK
                     ELMAFRKLNKENEEINVTYTKIATLLEVLVTLFVESVICVILGYGGWLVYRGQFNAGQ
                     LVEYIGYFEAIVWPIMAISMLIEKTARGKASLNRITELLDAPIDVADRDGVADLKNPR
                     GGIEFRHLNFRYPDGEYDVLKDISFTIAPGESVGIVGKTGAGKTALVDLLLRTYNVPD
                     GTLFVDGQDVNTVSIHSVRNACAYVPQDNFLFSDTIAHNIGFGVDDASREDIDRAAAL
                     ADVRDNIVDFKDGYETVLGERGVTVSGGQKQRISIARALLKNAPILILDDSVSAVDTR
                     TERIILDNLKTSRAGKTTLLIAHRISTVEQLDKIIFIEDGRVEAVGPHDELYRTCNEY
                     RRMVDLQKLEDEVGGDGNG"
BASE COUNT          541 a          710 c          700 g          503 t
ORIGIN      
        1 gctcacatgg aacgacacgc cctgcaagac ccactcgccg gggatgtagc tgaaccagac
       61 atcgcggaac tcaatttcgc ccttcacctc gtccaactca acggtgtcgg gcgcgtccac
      121 catcttgggc tgaatgtcca tgatggagaa caccttctcc gacgaggcca gcgccgattg
      181 cagccagttg aactgctcgg ccagattctg gatgggggtg aagaacttgg agatatacat
      241 ataaaaggta acgatagtgc cgccggaaat cgtctgcccc aaaaacgtcg cgccggtcag
      301 gtggcccatg ccgcccaggt aaaacagaca caggatcgag cagatgtaca gcatatacac
      361 cagcggccgg aacacgccga aaacaaaaat ctgctcctgg ttggccttgg caagccgctg
      421 gcttttctcg cggaactccg ccattttttc atcctcgcgg ccgaaaatct gcgtgacctt
      481 gataccggaa agattttccg acaggtaggt gttgatatcc gtcgtcgcgt ccttgacctt
      541 gcggtaggcg cggcgggaga atttgcggaa aatcaccgtg aaaatcacga tgaacggcac
      601 aaagcacagc accatcagcg tcagctcata gttgagggac agcatggcga tgagaatgcc
      661 gaggatgacg aagatgttct tggccagcgt gaccagcagg ttcgtgaaca tcatcgagat
      721 agcgttcgtg tcgttggtca cgcgggtgac gagcttgccg accgggattt cgttcagctg
      781 ctcgtgggcc agcgactcaa tgtgggtgaa cagatcttcg cgcaggtcgg agataatgcg
      841 ctggccgacg cgctgcaaaa tgacggcctg gaggtaggtg cttgccatcg aaaacaccag
      901 cacgcccgca tagacggcca cgcccgcaaa gaggcgcggc agcgcaaact ccccggccac
      961 cagttcttcc agcgcaccga cgatgagggg cgagataatg tcgtaggcaa tcgagaacag
     1021 catcaggaac agcacaacga caaattttgc ccagtaaggc ttggcgtacc cggccaggcg
     1081 gcgcaggatt tcgccgtcct tcatcgtgcg ctcaaagccc atcgtctcct ttttgtcctt
     1141 gacaagggcg taggcgaaaa tcaaaatggc cgtgaccgtg ccgatgacgc ccccgacgag
     1201 aagcagcgga ttcaggttag ccattgccgt cacctcccac ttcatcttcc agcttttgca
     1261 ggtctaccat gcggcggtat tcgttgcagg tacggtacaa ttcgtcgtgc gggcctacgg
     1321 cctccaccct gccgtcctcg ataaagatga ttttgtccag ctgctccact gtggaaatac
     1381 ggtgggcaat cagcagggtg gtcttgcccg cgcggctcgt tttcaggttg tcgaggatga
     1441 tgcgctcggt gcgggtatcg actgctgaga ccgagtcgtc cagaatcaaa atcggcgcgt
     1501 ttttcagcag tgcccgggca atcgaaatgc gttgcttctg cccgccggaa acggtcacac
     1561 cgcgctcgcc gaggacggtt tcgtagccat ctttaaagtc cacgatgttg tcccgcacat
     1621 cggccagcgc agcggcgcgg tcaatgtcct cccggctggc gtcgtccacg ccgaagccga
     1681 tgttgtgggc aatcgtgtcc gagaacagga agttatcctg cggcacatag gcgcaggcgt
     1741 tgcgcaccga atggatggac acggtgttca cgtcctgccc atccacaaac agcgtgccgt
     1801 cgggcacatt gtaggtgcgc agcagcagat ctaccagcgc ggttttgccc gcgccggtct
     1861 tgcccacgat gccgacgctc tcccctggtg cgatggtaaa ggaaatatcc ttcaaaacat
     1921 cgtactcgcc gtccgggtag cggaagttca gatggcggaa ctcgatgcca ccgcggggat
     1981 ttttcagatc ggccacgccg tcacggtcgg ccacatcgat gggcgcgtcc agcagctcgg
     2041 taatgcggtt cagcgatgct ttgccgcggg cggtcttttc aatcagcatt gaaatcgcca
     2101 tgatgggcca gacgatggcc tcaaagtagc cgatgtactc caccagctgg cccgcgttga
     2161 actggccccg gtagacgagc cagccgccgt agccgaggat aacgcagatg accgattcaa
     2221 caaacagagt gacgaggacc tccaacagtg tggcaatttt tgtataggta acgttgattt
     2281 cctcgttttc cttgtttaac ttgcggaacg ccatcagctc tttcagctct ttgacaaacg
     2341 ccttgatgac cgcaatgccg gagaaatttt cctgcgcaaa gtccgatagg tcggaaaatg
     2401 cctgctggcg ttcctcccaa cgcttggtca tgacctggct catcacggtg ccaa
//
LOCUS       DBMDSQ010000299         2452 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_9442_length_2452_cov_1.441514, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000299 DBMDSQ010000000
VERSION     DBMDSQ010000299.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 2452)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2452)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2452
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9442_length_2452_cov_1.441514"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(181..975)
                     /locus_tag="ACZPAX_09195"
     CDS             complement(181..975)
                     /locus_tag="ACZPAX_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015564878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1114112.1"
                     /translation="MLEIKNVYKTFNAGTVNEKVALKGLDLTLEDGDFVTVIGGNGAG
                     KSTMLNAVAGVWPVDMGKIVIDGQDVTRLSEHQRAKYIGRVFQDPMMGTAATMQIDEN
                     LALAARRGLARTLKTGITKKEHDEYYELLKTLDLGLENRMTSKVGLLSGGQRQAVTLL
                     MATLKKPKLLLLDEHTAALDPKTAAKVLTLSEKIVEENHLTTLMITHNMKDAIKYGNR
                     LIMMYEGHVIYDVKGEEKKNLQVSDLLQRFEQVSDGEFANDRMLLS"
     gene            complement(968..1915)
                     /locus_tag="ACZPAX_09200"
     CDS             complement(968..1915)
                     /locus_tag="ACZPAX_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007491899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1114113.1"
                     /translation="MDFLTSLINSMPGAVAQGLIWGIMAIGVYITYRILDVADLTVDG
                     SLGTGGAVCVVLVLNGAPVGVALVAATLAGMLAGLVTGLFHTACGIPAILAGILTQLA
                     LYSVNLRIMGTGTGGGKANLPISVDKYSLLVSARYVRQLALNNPILVLVIFCAVLIGV
                     LYWFFGTELGCSLRATGANQHMARAQGINTNITIVLGLMVSNGLVALAGGLLAQYQGF
                     SDINMGRGAIVIGLAAVIIGEVIFGKIFCNFALKLTAAVIGAIIYYIVMNFVLRMGLS
                     TDDLKLLTALVVAIFLTIPYWKGKYFTKTPVKKGGKDNA"
     gene            complement(2014..>2452)
                     /locus_tag="ACZPAX_09205"
     CDS             complement(2014..>2452)
                     /locus_tag="ACZPAX_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR1114114.1"
                     /translation="VYQCDVIEGYLTEEGYTVARYAFTDTNDVTSVAQSAADNSDVIY
                     IPTDNTAASNTEAIANVVLPAQVPVVAGEEGICSGCGAATLSISYYDLGYATGEMAAK
                     ILAEGADISTMPVEFAPNVTKKYNAANCEALGITPPDDYVAIG"
BASE COUNT          504 a          690 c          734 g          524 t
ORIGIN      
        1 cgagtacctc cttagtctat ggcaagtggg agccacaagg ccatatccca cctagtatac
       61 atactagaga ttatatcata tttagcgggc tttgaacatc gttttcaaaa aattgtcacg
      121 tattacaatt ttattacaaa gagaatgccc gggatggtgc atcccgggca aaattgtgga
      181 ttacgaaagc agcatacggt cgttggcaaa ctcgccgtcg ctgacctgct caaagcgctg
      241 cagcaggtcg ctgacctgaa ggttcttctt ttcctcgccc ttcacatcgt agatgacgtg
      301 gccctcgtac atcatgatca ggcggttgcc gtacttgatg gcgtccttca tgttgtgggt
      361 gatcatcagg gtggtcaggt ggttttcctc gacgattttt tcggagaggg tcagcacctt
      421 ggcggccgtc ttggggtcca gggccgcggt gtgctcgtcc agcaacagga gctttggctt
      481 tttcagcgtt gccatcagca gcgtgaccgc ctggcgctgg ccgccggaga ggagtcccac
      541 cttgctggtc atgcggtttt ccaaccctaa gtccagcgtt ttgagcagtt cgtagtattc
      601 gtcgtgctct ttcttcgtga tgccggtttt cagtgtgcgg gccaagcccc ggcgggccgc
      661 cagggccagg ttttcatcga tctgcatggt agcggcggtg cccatcattg ggtcctggaa
      721 cacgcggcca atgtacttgg cacgctggtg ctccgacagg cgggtcacat cctgcccatc
      781 aatgacgatc ttgcccatat ctacgggcca gacaccggcc acggcattca gcatcgtgga
      841 cttgcccgcg ccgttgccgc cgatgaccgt gacaaagtcg ccgtcctcca gcgtcaagtc
      901 gaggcccttt aaggcgacct tctcgttgac agtgcccgcg ttgaaggtct tgtacacatt
      961 tttaatttca agcattgtcc ttgcctccct ttttcaccgg cgttttcgtg aagtatttgc
     1021 ctttccagta cgggatggtc aggaagatgg ccacgaccag cgcagtcagc agcttgaggt
     1081 cgtcggtgga cagacccatg cgcagcacga agttcatgac aatgtagtag atgatagcac
     1141 cgatgaccgc cgccgtcaac ttgagggcaa agttgcagaa gattttgccg aagatgacct
     1201 caccgatgat gacagctgcc agaccgataa cgatagcgcc gcggcccatg ttgatatcgg
     1261 agaagccctg gtactgcgcc agcagaccgc ctgccagcgc gaccaggccg ttggagacca
     1321 tcaggcccag cacgatggta atgttcgtgt tgatgccctg ggcacgggcc atatgctggt
     1381 tcgcacccgt ggcacgcagc gagcagccaa gctcggtgcc gaagaaccaa tacagcacgc
     1441 cgatcagcac cgcgcagaag atgaccagca ccagaattgg gttattgagc gccagttggc
     1501 gcacatagcg tgcggagacc agcaggctgt atttgtccac gctaatgggc aggttggctt
     1561 tgccgccgcc tgtgccggtg cccatgatgc gcaggttgac agaatacagc gcgagctggg
     1621 tcaggatacc ggccagaatt gccgggatgc cgcaggccgt gtggaacagg ccggtgacaa
     1681 ggcccgccag cataccggcc agcgtggccg ccaccagggc gacgcccacc ggtgcgccgt
     1741 tcaggaccag cacaacgcag actgcgccac cggtaccgag ggagccgtcc acggtcagat
     1801 ccgccacatc cagaatacga taggtgatgt agacgccgat agccataatg ccccagataa
     1861 ggccctgcgc gacggcaccg ggcatcgagt ttatcaggga ggtcaggaaa tccataaaaa
     1921 catcctcatt tcataaagca ccaaaacagc ggggaaagag aggcgtctcc ccttcctcgc
     1981 tgttatgatg atttttagtc gctgttccgg ttatcagccg atggccacgt agtcgtccgg
     2041 cggcgtgatg ccgagggctt cgcagttggc ggcgttgtat ttcttggtga cgttgggggc
     2101 gaactcgacc ggcatggtgg agatgtcagc accctcggcc agaatcttag cggccatctc
     2161 accggtggcg tagcccagat cgtagtagct gatggacagt gtggcagcgc cgcagccgga
     2221 gcagatgcct tcctcaccag ccacaacagg aacctgcgcg ggcagcacga cattggcgat
     2281 agcctcagtg ttggaggcgg cggtgttatc ggtggggatg tagatgacat cggagttgtc
     2341 ggccgcgctc tgggcaacgg aggtaacgtc gttcgtgtcg gtgaaggcgt agcgggcgac
     2401 ggtgtagccc tcctccgtca ggtagccctc gatgacgtca cactggtaga ca
//
LOCUS       DBMDSQ010000300         2447 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_9472_length_2447_cov_1.631714, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000300 DBMDSQ010000000
VERSION     DBMDSQ010000300.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 2447)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2447)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2447
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9472_length_2447_cov_1.631714"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..971
                     /locus_tag="ACZPAX_09210"
     CDS             <1..971
                     /locus_tag="ACZPAX_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HIR1114115.1"
                     /translation="YPLIGNYGMNSEDTESGKIWAKGYIVREACKTPSNFRSEETLDS
                     FLKKNGIIGIEGIDTRSLTRTLRESGVMNGAITTEFDPDAEPEKKAALLPEIAAYAVV
                     NAVKTVTCREAKTYEPTQAGEWGDTPLHVALLDLGCKNNIVRCLQKRGCRVTVLPGMT
                     TAAELAKLNPDGLMLSNGPGDPAENVEIIANIREMLNTGIPTFGICLGHQLTALAAGA
                     KTCKLKYGHRGANQPVSHISKSRTFITSQNHGYAVEDETLSKDVGQMSYFNANDGTCE
                     GVDYFKWNCFTVQFHPEANGGPKDTEFLFDEFVSRMLAAKGVIDNA"
     gene            964..>2447
                     /gene="carB"
                     /locus_tag="ACZPAX_09215"
     CDS             964..>2447
                     /gene="carB"
                     /locus_tag="ACZPAX_09215"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005354269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HIR1114116.1"
                     /translation="MPKDPRIKKVLVIGSGPIIIGQAAEFDYSGTQACRALKSEGVET
                     VLVNSNPATIMTDPDIADHVYIEPLTAEVVERIIEKEKPDAILPNLGGQMGLNLSMEL
                     ARSGFLDRSGVRLLACKPDTIDRAEDRQLFKDTMEKLHQPIIPSKVVEDLDDALDFAE
                     VIGYPVIVRPAFTMGGTGGGICEDREKLHEIATNGLRLSPIHQILVEKCISGWKEIEY
                     EVMRDSKGNVITVCNMENLDPVGIHTGDSIVVAPSQTLSDKEYQMLRTAALDIITELG
                     IEGGCNCQFALKPDSFEYAVIEVNPRVSRSSALASKATGYPIAKVATKIALGYTLDEI
                     TNDVTGETCACFEPALDYVVVKYPKWPFDKFVYADKSLGTQMMATGEVMAIGNNFEHA
                     MMKAVSSTELGLDTMTLPDFEKLTTEEVIEHLHVQDSERAFCVYEALKRGVPHDVIYD
                     ITKIDWWFLDKLQHLADIEMGLKTGELTTEKYLNAKRFGFLDKT"
BASE COUNT          547 a          781 c          689 g          430 t
ORIGIN      
        1 cctatccgct catcggcaac tacggcatga acagcgagga tactgagagc ggcaagattt
       61 gggccaaggg ctacattgtg cgcgaggcgt gcaagacccc ttccaacttc cgcagcgagg
      121 aaacgctgga ttccttcttg aagaaaaacg gcatcatcgg cattgagggc atcgacactc
      181 gcagcctgac ccgcaccctg cgggagagcg gcgtcatgaa cggtgccatc accaccgagt
      241 ttgaccctga cgccgagccg gagaagaagg ccgcgctgct gcccgagatt gccgcttatg
      301 ccgtcgtcaa cgccgtcaag accgtgacct gccgcgaggc caagacctac gagccgaccc
      361 aggcagggga gtggggcgac acgcctctgc acgttgcact gttggatctg ggctgcaaga
      421 acaacatcgt gcgctgtctg caaaagcgcg gctgccgcgt gaccgtgctg cccggcatga
      481 ccaccgccgc cgaactggct aaacttaacc ccgacggcct gatgctctcc aacggccccg
      541 gcgacccggc agagaatgtg gaaatcattg caaacatccg cgaaatgctg aacaccggca
      601 tcccgacctt cggtatctgc ctgggccacc agctcaccgc gctggccgcg ggcgctaaga
      661 cctgcaagct gaaatacggt caccgcggtg ccaaccagcc ggtcagccac atcagcaaga
      721 gccgtacctt catcacgagc cagaaccacg gctacgcggt ggaggacgag acactgtcca
      781 aggacgtagg ccagatgagc tacttcaacg ccaacgacgg cacctgcgag ggtgttgact
      841 actttaaatg gaactgcttc accgtgcagt tccatcccga ggccaacggc ggccccaaag
      901 ataccgagtt cctgtttgac gagtttgtca gccgtatgct ggctgcgaaa ggagtgatcg
      961 acaatgccta aagacccgcg tatcaaaaag gtgctggtca tcggttccgg ccccatcatc
     1021 atcggtcagg cggcggagtt cgactactcc ggtacccagg cctgccgtgc gctgaaatcc
     1081 gagggcgtag agacggtgtt ggtcaactcc aaccccgcca ccatcatgac cgaccctgac
     1141 atcgccgacc atgtatatat tgagccgctg accgccgagg tcgtcgagcg catcattgaa
     1201 aaagaaaagc ccgacgccat cttgcctaac ctgggcggcc agatgggcct gaacctctcc
     1261 atggagttgg cccgcagcgg tttcctggac cgcagcggtg tccgtctgct ggcctgcaag
     1321 cccgacacca tcgaccgcgc cgaggatcgc cagctgttca aggacacgat ggaaaagctg
     1381 caccagccca tcatcccctc caaagtcgtg gaggatctgg acgacgcact ggactttgcc
     1441 gaggtcatcg gctacccggt catcgtgcgc cctgctttta ccatgggcgg caccggcggc
     1501 ggcatctgcg aggaccgcga gaagctgcat gaaatcgcca ccaacggtct gcgcctgtct
     1561 ccgatccacc aaatcctcgt agagaagtgc atttccggct ggaaggaaat cgagtacgag
     1621 gtcatgcgcg actccaaggg caacgttatc accgtctgca acatggagaa cctggatccc
     1681 gtcggcatcc acaccggcga ctccatcgtc gtggccccca gccagaccct ctccgataaa
     1741 gagtaccaga tgctgcgcac cgccgcgctg gatatcatca ccgaactggg catcgagggt
     1801 ggctgcaact gccagtttgc cctgaagccg gacagctttg agtatgctgt catcgaggtc
     1861 aatccccgcg tgtcccgctc ctccgcgctg gcatccaagg ccaccggcta tcccatcgcc
     1921 aaggtcgcca cgaagatcgc cctgggctat acgctggacg agatcaccaa cgacgttacc
     1981 ggcgagacct gcgcctgctt tgagccggcc ctcgactacg ttgtcgtcaa atacccgaag
     2041 tggccgtttg acaagttcgt ctatgcggac aagtctctcg gcacccagat gatggccacc
     2101 ggcgaggtca tggccatcgg caacaatttc gagcacgcca tgatgaaggc cgtctcctcc
     2161 accgagctgg gccttgacac gatgacgctg cccgactttg agaagctgac caccgaggaa
     2221 gtcattgaac acctgcacgt gcaggactcc gagcgcgcct tctgcgtcta cgaagccctc
     2281 aagcgcggcg tgccacatga tgtgatttat gacatcacca agatcgactg gtggttcctg
     2341 gacaaattgc agcacctggc cgacattgag atgggcctga aaaccggcga actgacgacg
     2401 gaaaaatatc tgaacgcgaa gaggttcggt ttcctggata agaccat
//
LOCUS       DBMDSQ010000301         2446 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_9481_length_2446_cov_1.374840, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000301 DBMDSQ010000000
VERSION     DBMDSQ010000301.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 2446)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2446)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2446
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9481_length_2446_cov_1.374840"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..381
                     /locus_tag="ACZPAX_09220"
     CDS             <1..381
                     /locus_tag="ACZPAX_09220"
                     /inference="COORDINATES: protein motif:HMM:NF013152.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HIR1114117.1"
                     /translation="PAGALTIGYGHNLDANPIEGLDALSVISEARAREILIADAAVFA
                     AALDEEIPWWRRLNAPRQAVLLDMAFNMGVGGLLKFGNTLRAAREMRWKDACDGMLAS
                     KWAGQVGRRASELAEQMMTGEWQG"
     gene            369..947
                     /locus_tag="ACZPAX_09225"
     CDS             369..947
                     /locus_tag="ACZPAX_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HIR1114118.1"
                     /translation="MAGLTVAFSLDMGGWAAAGVLAVALGARIMKIKASTARVMFYVY
                     AALMGFTLSSIFLVYDYGTLILAFLVGAVYFGVMAVYGNVTDRDLTGWGPKLMGGLVA
                     LIVCALVGSLLSMFGLNFGIADLLLCAVGLLIFMGLTAYDTQMLKYYYSYFGGDAAML
                     HKASIIGALNLYLDFINIFLYIVRMLGRRNND"
     gene            1048..1123
                     /locus_tag="ACZPAX_09230"
     tRNA            1048..1123
                     /locus_tag="ACZPAX_09230"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1081..1083,aa:Ala,seq:ggc)
     gene            complement(1371..2036)
                     /locus_tag="ACZPAX_09235"
     CDS             complement(1371..2036)
                     /locus_tag="ACZPAX_09235"
                     /inference="COORDINATES: protein motif:HMM:NF016961.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HIR1114119.1"
                     /translation="MENSNNTFLAAKAAIAAVCGAFTAAFGWLGWLVVAWAVCMALDW
                     LSGSAAAASRGKWSSAVARAGIWHKAGMLVVVVVAALTDAVLSIAVANLPGLGLTYSS
                     LILPVVLVWYIFTELGSIAENAAEMGANVPEWLLKLLAAGKSAADKSAGGITVETGKN
                     ADGSPIGHLDTAQLDELTTEDLEQLAIDMGLTVQDGAKRADLIAQISTEPVKVLGSGK
                     QKV"
     gene            complement(2051..>2446)
                     /locus_tag="ACZPAX_09240"
     CDS             complement(2051..>2446)
                     /locus_tag="ACZPAX_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HIR1114120.1"
                     /translation="GGYKHCHLEVRATAGGTGLDPTAYAGCENAVGIYGAADDGQTEQ
                     ISETPTGKTMQCLMIGPLDTRGMNKCDALAVKLRLISASRYYVLPVGTETYCVCVGAV
                     SNGDAVSFYQLAEAEGWTKDNKYLARYVG"
BASE COUNT          453 a          777 c          688 g          528 t
ORIGIN      
        1 cccgccgggg cgctgaccat cgggtacggg cacaatctgg acgccaaccc catcgagggg
       61 ctggacgcgc tttccgtcat ttccgaggcg cgggcgcggg aaatcctgat tgcggacgca
      121 gccgtgtttg ccgccgcgct ggatgaggag atcccgtggt ggcgcaggct caatgcgccg
      181 cgtcaggccg tgctgctgga catggccttc aacatgggcg tgggggggct tctgaaattc
      241 gggaacaccc tgcgggcggc gcgcgagatg cgctggaagg acgcctgcga cggcatgctg
      301 gcctcgaagt gggccgggca ggtcgggcgg cgcgcgagcg agcttgcgga acagatgatg
      361 accggcgaat ggcagggctg accgtggcgt tctctctgga tatgggcggc tgggcggccg
      421 ccggagtgct ggccgtggcg ctgggcgccc gcatcatgaa gatcaaggca tcgaccgccc
      481 gtgtcatgtt ctacgtgtac gcggcgctga tgggattcac tctcagctcc atcttcctgg
      541 tgtacgacta cggcacgctg atcctggcgt tcctcgtcgg tgcggtctac ttcggcgtca
      601 tggctgtgta cggcaatgtc accgaccgcg acctgaccgg ctggggtccc aagctgatgg
      661 gcggcttggt cgcgctgatc gtctgcgcac tggtgggcag tctgctgagt atgttcggcc
      721 tgaacttcgg cattgcggac ctgctgctct gcgccgtcgg tttgctgatt ttcatgggcc
      781 tgacggcgta tgacacccag atgctgaaat attactactc ctacttcggc ggggatgcag
      841 ccatgctgca caaagcgtcc attatcggcg cgctgaatct ttacctcgat ttcatcaata
      901 tcttcctgta tattgtccga atgctgggcc gccgtaacaa cgactaaaaa ccttgaaaaa
      961 attcaggaaa acgcttgaca ttctgaaaac ggtttgctat aataaatggc gtcgtcagga
     1021 gcgggtgctt cacgacgacg ccagcatggg ggattagctc agctgggaga gcgcttgcat
     1081 ggcatgcaag aggtcaccgg ttcgatcccg gtattctcca ccattagcta cgaatccgaa
     1141 ccaaatactt attattggtt tggttctggt tcagacaagg aagcatctta tggtttagtg
     1201 ccgtaaggtg cttttgcttt tcatatcaac aaagagaatg ccccgcgctg ttacagtgca
     1261 gggcattctc tttgttggaa taaatctcca actaggattg ttagcatttc ctagtatacc
     1321 cgactattcc aatcattgca accacattcc aaatttgctt caaacttgct tcaaactttc
     1381 tgcttaccgc taccaagcac cttcaccggc tctgtactga tctgcgcaat caaatccgca
     1441 cgcttcgcgc cgtcctgcac agtcagaccc atgtcaatcg ccagctgctc caagtcctcg
     1501 gtcgtcagtt catccagctg tgccgtgtcc agatgcccaa taggggagcc gtccgcattt
     1561 ttcccggttt ccacggtgat accgcccgcg ctcttgtccg ctgcgctttt cccagcggca
     1621 agcagcttca acagccactc cggcacgttc gcgcccattt cggcggcgtt ttcggcaatg
     1681 gagcctagct cggtaaaaat gtaccacacc agcaccaccg gcagaatcaa gctcgagtac
     1741 gtcagcccca gccccggcag gtttgccacg gcgatgctca acacagcgtc cgtcagcgcg
     1801 gccactacga ccaccaccag catccccgcc ttgtgccaga tacctgcgcg ggcaacggcg
     1861 ctcgaccact tgccgcggct ggccgccgct gcactgccgc tgagccagtc cagcgccatg
     1921 cagacagccc aggcgactac cagccacccc agccagccaa acgccgccgt gaacgcccca
     1981 cagaccgccg caatggccgc tttcgccgcc aaaaaggtat tattgctgtt ttccatatct
     2041 ttgccctcca tcagcccaca taccgggcca aatatttgtt gtctttcgtc cagccctcgg
     2101 cctcggccag ctggtagaag ctcaccgcgt caccgttgct gacagcgccg acgcagacgc
     2161 agtacgtctc cgtgccgacc ggcagcacat agtagcggct tgcgctgata agccgcagct
     2221 taacggccag cgcgtcgcat ttgttcatgc cccgcgtgtc cagcggccca atcatcaagc
     2281 actgcatcgt ttttccggtc ggcgtctcgc tgatctgctc ggtctggccg tcgtccgccg
     2341 cgccgtagat tcccaccgcg ttctcacacc cggcgtaggc cgtggggtcc agcccggtgc
     2401 cgcccgccgt ggcccgcacc tccagatggc agtgcttgta gccgcc
//
LOCUS       DBMDSQ010000302         2440 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_9515_length_2440_cov_1.383497, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000302 DBMDSQ010000000
VERSION     DBMDSQ010000302.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 2440)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2440)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2440
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9515_length_2440_cov_1.383497"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1459)
                     /locus_tag="ACZPAX_09245"
     CDS             complement(<1..1459)
                     /locus_tag="ACZPAX_09245"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF013100.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HIR1114121.1"
                     /translation="MPQPNNQPKKHRTRANAAMKTRTLFCVTLFIVGAFGLLIYQLYA
                     LQLRDAELYRTEAVTQQLKDTTLPALRGSIYSVNGKLLAKSSTVWNIVADPSSIAKSG
                     ASESQLRTAAENIAALLGGDTTADTVYEVLTAKNSSGEPYQYRMLAKGVDKPVVDSIL
                     NYADEYRMDPEDGAATGKRILYFSTEQATTRSYPYGEFLSSVLGFCNSDGEGAYGLEK
                     YYDETLAGTPGRSVAETDVYGNALAAGQADVHEAIDGNNLTLTIDENVQSIVEEYLTE
                     AMNTFTVHGRGSAIVMNVKTGAILAMASIEQFDPNDPYKITDTKMTDILEKEEIDAND
                     IDWLEGRLGEKAVADIVADGKISREKTIGEDGKEVASEYTQLQGMMREAQWKNKNITE
                     LYMPGSVFKLITASAGLDSGVMSTSQTFYCGGSLTVNEGSELWEHTYRCANGEVHRMQ
                     DMAGALNNSCNLWFIQAAETLKPQIFYDYIQAFGFT"
     gene            complement(1545..2021)
                     /locus_tag="ACZPAX_09250"
     CDS             complement(1545..2021)
                     /locus_tag="ACZPAX_09250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0032153 - cell division site [Evidence
                     IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsB/FtsL family cell division protein"
                     /protein_id="HIR1114122.1"
                     /translation="MTTVLLHSCAAAPKLETPRPQVKVVRGGNRGLNRARRFAQATLR
                     TLALAIVLGLVVSVLYSHAKLTELSGQINEVNTQLTAAQSEYDYLSTKMSDITSRASL
                     QEVAEGQLGLVKLDPSQITYVQLEDQSIIEKSSSSAVRLLDKVRTAALSLLDGLNP"
     gene            complement(2032..>2440)
                     /gene="mraW"
                     /locus_tag="ACZPAX_09255"
     CDS             complement(2032..>2440)
                     /gene="mraW"
                     /locus_tag="ACZPAX_09255"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015558502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="16S rRNA
                     (cytosine(1402)-N(4))-methyltransferase"
                     /protein_id="HIR1114123.1"
                     /translation="KRVLVRSLNNPTVGSTKASVIRAKNPARRTFQAIRIAVNSELDA
                     LNEGLDAIFNCLAPGGRLCVITFHSLEDRLVKNKFRRWAQRCTCPPEQPICTCGGVAK
                     AKLITRKPIEADAAELETNRRSRSAKLRVLEKV"
BASE COUNT          415 a          696 c          748 g          581 t
ORIGIN      
        1 gcgtgaagcc aaacgcctgg atatagtcgt agaagatctg cggtttcagt gtctcggcag
       61 cctgaatgaa ccagaggttg cagctgttgt tcagcgcacc cgccatgtcc tgcatacggt
      121 gaacctcgcc gttggcgcag cggtaggtgt gctcccatag ttcgctgccc tcattgacgg
      181 tcaggctgcc gccgcagtag aaggtctggc tcgtgctcat gacgccggag tccagtcccg
      241 ccgatgcggt gatgagctta aagacagagc cgggcatata cagttcggtg atgtttttat
      301 tcttccactg ggcctcgcgc atcataccct gtaattgggt gtactcgctg gcgacctcct
      361 tgccgtcctc gccgatggtt ttttcgcggc tgatcttgcc gtcggccacg atatcggcca
      421 cggctttttc gcccaggcgg ccttccagcc aatcaatatc gttggcgtcg atttcctctt
      481 tttccaaaat gtcggtcatt ttggtatcgg tgattttgta cggatcgttg gggtcaaact
      541 gctcgatgga cgccatggcc aaaatcgcgc cggtcttgac gttcatgacg atagcgctgc
      601 cgcggccatg caccgtgaag gtgttcatcg cctcggtcag gtattcttca acgatggact
      661 gcacattttc gtcgatggtc agggtcaggt tgttgccgtc gatagcctcg tgcacgtcgg
      721 cctgcccggc ggccagggcg ttgccataga catccgtctc ggccacactg cggcccggcg
      781 tgcctgccag ggtttcatcg tagtattttt ccaggccgta agcgccctcg ccatcgctgt
      841 tgcagaagcc cagcaccgag gagaggaact cgccgtaggg gtagctgcgg gtcgtggcct
      901 gttcagtcga aaagtacaaa atgcgtttgc cggtcgcggc gccgtcctcg ggatccatgc
      961 gatactcatc ggcatagttc aggatggagt ccaccaccgg cttatccacg cccttggcga
     1021 gcatacggta ctgatacggc tcgccgctgc tgtttttagc ggtcagaacc tcatagacgg
     1081 tatccgcagt ggtgtccccg cccagcagtg cggcaatgtt ttccgccgcc gtgcgcagct
     1141 ggctctccga cgcgccgctc ttggcgatag aggaagggtc tgcaacgata ttccataccg
     1201 tgctgctctt ggcaagcagc ttgccgttga cgctgtaaat cgagccgcgc agcgcgggca
     1261 gcgtcgtatc cttcagctgc tgggtcacgg cctcggtgcg gtagagttcg gcatcacgca
     1321 gctgcagggc gtagagttga taaatcagca gaccgaatgc gccgacgata aagagcgtca
     1381 cgcagaagag cgtgcgggtt ttcatcgctg cgttggcgcg ggtgcggtgc ttttttggtt
     1441 ggttgtttgg ttgtggcata gggtgttcct tatttcaaaa agcctttttg ccgtcggcca
     1501 tcgcgccgcg cagcaaaaag gctcgtagct gcataatttt attgttacgg attcaaccca
     1561 tccagcaagc tcagggcggc ggtgcggacc ttgtccagca ggcggacggc ggaactgctg
     1621 cttttttcga ttatgctctg atcttccaac tggacatagg tgatttggct cggatcgagc
     1681 ttgaccagtc ccagctggcc ctcggccacc tcctgcaggc tggcgcggct tgtgatatcg
     1741 ctcattttgg tgctcaggta gtcgtactcg ctctgggcgg cggtcagctg ggtgttgacc
     1801 tcattgatct gaccgctcaa ctcggtgagc ttggcgtggc tgtacagcac gctgacaacc
     1861 agccccagca caatggcaag agccagcgtg cgcagcgtgg cctgcgcaaa acggcgcgcg
     1921 cggttcagcc cgcggttgcc gccgcgcacc acttttacct gcgggcgcgg ggtctctaat
     1981 ttaggtgcag ccgcacagct gtgcagcaaa acggtcgtca tggtttgcct cttatacttt
     2041 ctccaacact ctcagtttgg cgctgcggct gcggcggttg gtttccagct ccgcagcgtc
     2101 agcttcgatg ggtttgcggg taatgagctt tgcctttgcc acacccccgc aggtgcagat
     2161 gggctgttcc ggcgggcagg tgcagcgctg tgcccagcga cggaacttgt tcttgactaa
     2221 gcggtcctcc aagctgtgga aggtgatgac gcagagccgc ccgcccgggg ccaggcagtt
     2281 gaaaatcgca tccaggccct cgttcagggc gtccagttcg gagttgacgg cgatgcggat
     2341 ggcctggaag gtgcggcggg ccgggttttt ggcgcgtatc acggatgcct ttgtcgatcc
     2401 tacagtggga ttattgagtg accgcaccaa gacacgtttc
//
LOCUS       DBMDSQ010000303         2438 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_9524_length_2438_cov_1.881044, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000303 DBMDSQ010000000
VERSION     DBMDSQ010000303.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 2438)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2438)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2438
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9524_length_2438_cov_1.881044"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1371)
                     /locus_tag="ACZPAX_09260"
     CDS             complement(<1..1371)
                     /locus_tag="ACZPAX_09260"
                     /inference="COORDINATES: protein motif:HMM:NF012321.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HIR1114124.1"
                     /translation="MQHKFLTLAAAVAAAWVFGLPALAAGGAGVYGTVQGQDAVLYLR
                     SGSADAAYTCMVGNTPVTPAKATPLSGLDTPAHTVILLDNSAAIPESQRDTIHDILNG
                     LAGTRLNGETFTLDTLGAEVTPLCDGETDYLQLKTVVDSLTYNDHTAQLTDCVYRVLD
                     SLVQQNSPALTRVVLITTGAEEEQYGYTRQELADLIRKAGFPLYIIGADDGTDAARAQ
                     LQELFALARLTDGDTYALSETQDVAAIVNGIRAWDRGVRVDVPLPAALCDGSDRAMQL
                     SDGGANTATLTLTMPFAAADNTTSDVGRDAPIPPQDTADTAPTAEPEPLVQPTAPDED
                     KPGVLPFALGGAGLLAALAAAFMALRRKKPAPAAPLPVQPAASDAPATMMAEATMFGG
                     TAAIFGGDAAPLTVTLTDLADPAHTVTFPLDGMVSLGRDPGLCRIVLDYDPSISRHQC
                     DLCLRGG"
     gene            complement(1373..1594)
                     /locus_tag="ACZPAX_09265"
     CDS             complement(1373..1594)
                     /locus_tag="ACZPAX_09265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1114125.1"
                     /translation="MNDTTKNMRTAEQLAALADTLERQAAAPLGEAAAALHLAWQSDA
                     AVQFLRRQQAVTDDLRAAARTLRQLAEEA"
     gene            complement(1607..1825)
                     /locus_tag="ACZPAX_09270"
     CDS             complement(1607..1825)
                     /locus_tag="ACZPAX_09270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1114126.1"
                     /translation="MADYNETLRALRRDAAELERIAGGTGACWTGAAAENFRRQFTAQ
                     TCQLADLLDTLGAAAGDETPALPTEFIL"
     gene            complement(1837..2085)
                     /locus_tag="ACZPAX_09275"
     CDS             complement(1837..2085)
                     /locus_tag="ACZPAX_09275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1114127.1"
                     /translation="MAANMKVNTRRLGEDIAALSDRLAAMQRTAAELAPALDAPRADA
                     LTEQLDRLDALLDRLDEIQTTYDVCETAVTRAVESIRL"
     gene            complement(2099..>2438)
                     /locus_tag="ACZPAX_09280"
     CDS             complement(2099..>2438)
                     /locus_tag="ACZPAX_09280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1114128.1"
                     /translation="FTVHTGALRTMADSCTAAARAVESVCADLPPAARTLADRLESDT
                     AALRRLADAAEEIAALYEQAEQRAAADELRTLGAFAPARPGMEPPAPDPAPALTPMLG
                     ETVMARFRDA"
BASE COUNT          354 a          790 c          879 g          415 t
ORIGIN      
        1 tccgccgcgc aggcagaggt cgcactggtg gcgcgagatc gaggggtcat agtccagcac
       61 gatgcggcac agccccgggt cgcgcccgag ggagaccata ccgtccagcg ggaaggtgac
      121 agtatgggcc gggtcggcca ggtcggtcag ggtcacggtc agcggggcgg cgtccccgcc
      181 aaaaattgcc gcagttccgc caaacatggt cgcctcggcc atcatggtgg cgggtgcgtc
      241 cgacgcggcg ggctgcaccg gcagcggcgc ggcgggggcg ggctttttgc ggcgcaatgc
      301 cataaacgcg gcggccaagg cggccagcag acccgcgccg cccagggcaa aaggcaggac
      361 accgggtttg tcttcatctg gggcggtggg ctggaccagg ggttctggct cggcagtcgg
      421 ggcggtgtcg gcagtgtcct gcggcgggat gggggcatcc cgccctacat cggaggtggt
      481 attgtccgcc gccgcaaacg gcatggtcag cgtcagcgtc gcggtgttgg ctccgccgtc
      541 ggaaagctgc atcgcccgat cagagccatc gcaaagggct gcgggcagtg gtacatccac
      601 ccgcacgccg cggtcccagg cacggatgcc gttgacgatg gcggccacat cctgtgtctc
      661 gctcagtgcg taggtgtcgc cgtcggttag gcgggccagg gcgaacagct cctgcagctg
      721 cgcccgggcg gcgtcggtgc cgtcgtccgc gccgatgatg tacagcggga acccggcctt
      781 gcggatgagg tcggccagtt cctgccgggt gtagccgtac tgttcttcct cggcaccggt
      841 ggtgatgagc acgacccgcg tcagagcggg gctgttttgc tgcaccaggc tgtccaacac
      901 gcggtaaacg cagtcggtca gttgggcggt gtggtcgttg taggtcaggc tatccaccac
      961 ggttttcagc tgcaaatagt ccgtctcacc gtcacaaagc ggcgtgacct cggccccgag
     1021 ggtgtccagc gtgaacgtct cgccgttcag gcgcgtgccg gccaggccgt ttaaaatatc
     1081 gtggatggta tcccgctggc tctccgggat ggcggcagag ttgtccagca gaatgacggt
     1141 gtgggccggg gtgtccagcc cggacagcgg cgtggccttg gcgggcgtga ccggcgtgtt
     1201 gcccaccatg caggtgtagg cggcatcagc gctgccgctg cgcaggtaca aaacggcgtc
     1261 ctggccctgc acggtgccat agacccccgc cccgcctgcg gccaacgcgg gcaggccaaa
     1321 cacccaggca gccgccacag ccgccgccag ggtcaggaat ttgtgttgca tcctatgcct
     1381 cctccgccag ttggcgcaat gtgcgggcgg cggcacgcag gtcgtcggtc acggcctgct
     1441 ggcggcgcaa aaactgcacg gcagcgtcac tctgccaggc caggtgcagc gcggcggcag
     1501 cctcgcccaa tggggcggca gcctggcgct ccaaggtgtc tgccagggcg gccagctgtt
     1561 cggcagtgcg catgtttttc gttgtatcgt tcatattcaa gttcccttac agaataaatt
     1621 ccgttggcag ggcgggggtc tcgtccccgg ctgccgcgcc caatgtgtcc agcaggtccg
     1681 ccagctggca ggtctgggca gtgaactggc ggcggaaatt ctccgccgct gcgccggtcc
     1741 aacaggcccc ggtgcccccg gcaatgcgct ccaactcggc cgcgtcgcgg cgcagagcgc
     1801 gcagagtttc gttgtaatct gccatcgttg ccctcctcac agccggatgc tctcgaccgc
     1861 gcgggtcacg gcggtctcgc agacatcgta ggtggtctgg atttcgtcca ggcggtccag
     1921 cagggcgtcc agccggtcca gctgctcggt cagcgcatcg gcgcggggtg cgtccagcgc
     1981 gggggccagc tcggcggcgg tgcgctgcat cgcggccaaa cggtcgctga gggcggcgat
     2041 gtcctcgccc aagcggcggg tgtttacctt catatttgct gccattcctc tgccctcctt
     2101 atgcgtcgcg gaaccgcgcc atgaccgtct cgcccagcat cggcgtcagg gccggggcgg
     2161 gatccggggc gggcggctcc atgccgggcc gggccggggc aaaggccccc agggtacgca
     2221 gttcgtcggc agcggcgcgc tgttcggcct gctcatacaa tgcggcgatc tcctccgccg
     2281 catctgccag gcggcgcagg gcagcggtat cgctttccag cctgtcggcc agggtgcggg
     2341 cagcgggcgg caggtcggcg cagacgcttt ccactgcacg ggcggcagcg gtgcagctgt
     2401 cggccatcgt gcgcagggcc ccggtatgta cggtaaat
//
LOCUS       DBMDSQ010000304         2419 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_9621_length_2419_cov_1.837360, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000304 DBMDSQ010000000
VERSION     DBMDSQ010000304.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 2419)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2419)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2419
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9621_length_2419_cov_1.837360"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          487 a          610 c          771 g          551 t
ORIGIN      
        1 cggtttcctt attcagacga tgtatgtgtc tcctgttgct gttcttaatc ttgcgcggct
       61 ttcttgcgtg caatcagcac aactgccgca actccgataa ccagcagcgc caatgctccg
      121 taggcgatgc tttccgttac atgcaggctc ttggggtcga agcgcatctc gatggtatgt
      181 ttgccggcag gcacatatag ggtgcgcagc acgtaatccg cacgtcccag ttcggccggt
      241 tgcccgtcga tggttacctg ccagccgtcc ggatagtata tctcggagaa tacggcgata
      301 ccgtcgcccg cattgtccgt ttcgtaaacc aggcggttgg gctcatagct tgtcaggcgg
      361 atggaggaaa ggctgtcttt gtaggcttcg gcagtacctt tcagcacatc tttgaaacgg
      421 gtgtcgacaa cggcggtttc ggtgggaaga atggttttga gcgcatcgat ttcctcattg
      481 gcgttgttta cgtattgcac gttctttaca aaccaggcgt tgccgttggc ataggggttg
      541 aggacgggaa cggtctgccc ctgctgtccg gcggggaaga tgaagtactt ggtgttcagc
      601 atgttcagta cgcggaattt ggaagcgtcc acgctgtcca tttctccgcc tgcggcggct
      661 atcgcttggt aggcggcctg catctcggga gagatatgac gctctatcag ttcctggtag
      721 cggcgcagtt tggcggcatg atacccgcct atgttcttgt gccagtagga agtattgttc
      781 tcgttgaacg cgtcggtggc aaagttcagc acacggtagt cgggagcttt gtcctgcaga
      841 atcagctcgt cggttttggt tttggtgaat gtttccgtcc ggatggaacg gggcacgaac
      901 tggtcgtcgt gcaggtaacg tttgtttacg gtccacatgt cggcaaggca gagcgccgta
      961 atgcctgcaa cggtcagtga ccggcgcagc ttgccggccg agtggagcca aagcagtgcg
     1021 catccgatgc ctataatgat gaagctccgc aatgcgtcgg cgctgacaag cgcgccccgc
     1081 atttccgcaa ggttggcgag gatgccggac agctcgttgg cgggtatcat ctgctggtcg
     1141 acggcatttt gcagcatctg cgcctcttgc gcgggcacga agcctgagcc gaggctgccc
     1201 ggcgcaatga caagcagcag ggctacgccg gcggtgagcg taaggctgat gccgaaggct
     1261 ttcttgttcg ttttccatat gccgggctct tccaatacac gcttcagggc gaaaatcgcg
     1321 agcaggggaa tggtaaactc agctatgacg aggatggacg atacggcgcg gaacttgttg
     1381 tacatcggga tgtagtcgat gaagaagtcc gtcagcggca tgaagttctt accccatgcc
     1441 aaaacgatgg agaagaaggt tgctcccagc aacgcccact tcagagggcc tttgacgatg
     1501 aagcagccca gcacgaagag gaacaggacg aatgcgccca cgtagacggg gccggccgtc
     1561 cagggctggc tgccgaagta ctgcgggaac gagttgtaga ggctgccgta catcggattg
     1621 gcctttccca tggcggtttc gctctggctg aggggggcgg cggaagagcc gcctttgaag
     1681 ttggggacga ggagtgtcca ggtttcgccg atgccgtagc tccagttggt gatgtagtcg
     1741 cggtccagtc cgctgctggt ttgcttggcg gcattgccgg tctgcacgag ctcgctcttg
     1801 ccgcgcatgg tttccttgct gtacgtgtag gtgtggtaga ggttggagag gttggcggcc
     1861 acgccgataa gggcggcgac gagcagtacg gcgcttgcct tgaagaatcc gggcagcgtc
     1921 ttgttgcgcc atgcgtcttc aaagtatgca cctgcgatga acagaatgac gaacaggaaa
     1981 tagtaggaca tctgcacatg gttcgacatg atttgcaggg cgatgaaaaa agccgtcagg
     2041 ataccgcccg ccagcagcct gccccggtag gcgagtacga tgcccgctat tgtgggcggg
     2101 atataggcga gggtgatgaa cttccagatg tgtcccgccg aaatcagaat gaagaagtag
     2161 gacgagaaag cccacatgat gccgcccaac cctgccagcc atgccggtat gccgaatacc
     2221 cgcaacagga tgtagaagcc cagcatcagg atgaacgtca ggttgacgta ggtgggcagg
     2281 aagagctggt atattttctg tgtccattgc aacggcacgg tagagtcgta agacggcgct
     2341 atctggtaag tcggcattcc gccgaaaagg gaattggtcc agcgggtgcg ttcgcctgtc
     2401 tgttcgtaat actctttgg
//
LOCUS       DBMDSQ010000305         2419 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_9622_length_2419_cov_1.766178, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000305 DBMDSQ010000000
VERSION     DBMDSQ010000305.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 2419)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2419)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2419
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9622_length_2419_cov_1.766178"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1052
                     /locus_tag="ACZPAX_09290"
     CDS             <1..1052
                     /locus_tag="ACZPAX_09290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1114129.1"
                     /translation="CAVTEEKTLTAETVAALPGAGESVLDITGSFAPGGVTLATGQAS
                     VQGTLQIQICSQNADSGELTVRSKELPIQQNLELPGAAEDDTALVRGEVLACTLSADD
                     GAGEAALTVTWKLRIEVWRSVQYSVVADAYSTLCKTQTLQTVCRLLQQTTALAGRIPI
                     SIEDDLPDAGGTVLGCFVTLGALCAAPAEGDKAETHLRLSGKGTAHVFISDARGELTC
                     YDKTFSWQPGGLWSGVADAAWPSVGASVVRVSSSKNGARLRVDLEIEVNGALLQAQSF
                     DALCGVELGEAYDDSDSPALYIYYAQQGERIFDIAKRYHARARDLAAANHLESSEPTR
                     ETTADAACLLIPAAL"
     gene            1068..>2419
                     /gene="spoIVA"
                     /locus_tag="ACZPAX_09295"
     CDS             1068..>2419
                     /gene="spoIVA"
                     /locus_tag="ACZPAX_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage IV sporulation protein A"
                     /protein_id="HIR1114130.1"
                     /translation="MTQEQIYHNIAQRTGGDIYLGVVGPVRSGKSTFIKRFAELMLLP
                     RIKNEARRARAHDELPQSAAGRTIMTTEPKFIPEKAVKIDLSGGGSFKARLIDCVGYM
                     VDGALGHEENEVPRLVKSPWFDHEVPFDLAAETGTRCVIREHATIGLVVTTDGSVADL
                     PREAYLDAERRIIAELNDSGKPYVILLNCAEPDSDDARRLATELAERYAHAVVPLNCT
                     TMTVETLDSLLQTLLYEFPIREIAVRMPGWVTMLESGHWLQSAIYTAMLEFAASVHKM
                     ADLAGRRPQLGCEYITSASLTGMDLASGSVGINAVVAPDIFYKILGEQTGLDIVDEAS
                     LLPCVVSLARAKRAYDKIKSALDQVEATGYGIVMPGIDELTLEEPEIVRQGGQYGVRL
                     SASAPSLHIIRANIHTELSPTVGSEQQGEELIKSLLSDFETAPGKLWSTNIFGKSLNE
                     L"
BASE COUNT          479 a          795 c          748 g          397 t
ORIGIN      
        1 agtgcgccgt gaccgaggaa aagaccctga ccgccgagac cgtcgccgca ctgcccggtg
       61 caggggagag cgttctggac atcaccggca gttttgcccc tggcggcgtg acccttgcca
      121 ccggtcaagc cagtgtgcag ggcactttgc agatacaaat ttgcagccag aacgccgaca
      181 gcggcgagct gaccgtgcgc agcaaggagc tacccatcca gcagaatttg gaactgcccg
      241 gcgccgccga ggacgacacc gcccttgtgc ggggcgaggt gctggcctgc actttaagtg
      301 ccgacgacgg cgcaggggag gccgccctca ccgtcacctg gaagctgcgc atcgaggtct
      361 ggcgcagcgt gcagtacagc gtcgtggcgg acgcctactc cacgctctgc aagacccaga
      421 ccctgcaaac cgtctgccgc cttttgcagc aaaccacagc gcttgcaggc cgcatcccca
      481 tcagcattga ggatgatttg cccgacgcag gcggcacggt cctgggttgt tttgtgacac
      541 tgggtgcgct ctgcgctgcg cccgccgagg gcgacaaggc cgaaacgcat ctgcggctgt
      601 cgggcaaggg gaccgcccat gttttcatca gcgatgcccg gggcgaactg acctgctatg
      661 acaaaacttt cagctggcag cccggcggcc tgtggtccgg tgtggccgat gccgcctggc
      721 ccagcgtggg ggcgtccgtc gtgcgggtct ccagcagtaa aaacggtgcc cggctgcggg
      781 tcgatctgga aatcgaagtc aacggtgcgc tattgcaggc acagagcttt gacgcgctgt
      841 gcggcgtgga gctgggcgag gcctacgatg acagcgacag cccggccctc tacatctact
      901 atgcccagca aggggagcgt atctttgaca ttgccaagcg ctaccacgcc cgcgctcggg
      961 acctggccgc cgccaaccac ctggaaagca gcgagccgac ccgggagacc acggccgacg
     1021 ccgcctgcct gctgataccg gctgcgctgt aaaggagggg aacatccgtg acacaggagc
     1081 aaatttacca caacattgcc cagcgcacag gcggggatat ctaccttggc gttgtggggc
     1141 cggtgcgcag cggaaaaagc acctttatca agcgctttgc cgaattgatg ctgctgcccc
     1201 gcatcaagaa cgaagcccga cgcgcccgcg cccacgacga gctgccccaa tccgccgccg
     1261 ggcgcactat catgaccacc gagccaaagt tcatccccga aaaagccgtc aaaatcgacc
     1321 tttcgggcgg cggcagtttc aaagcgcggc tcatcgactg cgtcggctac atggtagacg
     1381 gtgccctcgg acacgaggaa aacgaggtcc cgcgcctcgt caaaagccct tggttcgacc
     1441 acgaagtccc ctttgacctg gcagccgaaa ccggcacccg ctgcgtcatc cgggagcacg
     1501 ccaccatcgg gctggtcgtc acgacggacg gctcagtggc cgacctgccg cgggaagcct
     1561 accttgacgc cgagcggcgc atcattgccg agctgaacga cagcggcaag ccttatgtca
     1621 tcctgctcaa ctgcgccgag ccagacagtg acgacgcccg ccgcctcgcc actgaactgg
     1681 ctgagcgcta cgcccacgcc gttgtgccgc tgaactgcac gacgatgacc gttgagacgc
     1741 tggattcact cttgcagacg cttttgtacg agtttcccat ccgggagatc gccgtgcgga
     1801 tgcccggctg ggtcacgatg ctggaaagtg gccactggct gcaaagcgcc atctatacag
     1861 ccatgctgga atttgccgcc tcggtacaca aaatggccga cctggcaggc cgccgccctc
     1921 agctggggtg cgagtatatc acatccgcgt cgctcaccgg catggacctt gccagcggca
     1981 gtgtgggcat caacgccgta gtggccccgg atatctttta caaaatcctt ggtgagcaga
     2041 ccgggctgga catcgtggac gaagccagcc ttttgccctg cgtcgtcagc ctggcccggg
     2101 ccaagcgcgc ctacgacaag atcaaatccg ccctcgacca ggtcgaagcc accgggtacg
     2161 ggatcgtcat gccgggcatc gacgagctga cgctggaaga gccggagatt gtgcgccagg
     2221 gcgggcagta cggcgtgcgg ctttccgcca gtgcgcccag tctgcacatc atacgggcga
     2281 atatccacac cgagctttcg cccaccgtcg gcagcgagca gcagggcgaa gagctcatca
     2341 agagcttgct ctccgacttt gagaccgccc ccggcaagct gtggagcacg aatatcttcg
     2401 gcaagagcct gaacgagct
//
LOCUS       DBMDSQ010000306         2418 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_9629_length_2418_cov_1.755287, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000306 DBMDSQ010000000
VERSION     DBMDSQ010000306.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 2418)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2418)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2418
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9629_length_2418_cov_1.755287"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..806)
                     /locus_tag="ACZPAX_09300"
     CDS             complement(<1..806)
                     /locus_tag="ACZPAX_09300"
                     /inference="COORDINATES: protein motif:HMM:NF013324.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HIR1114131.1"
                     /translation="MRLLKIDVKTPETGVKTKEGVSLWLDSVVTVQVYSENSTVADEE
                     VRNAGYDDVKKYINSRQQAAISNFLGMDEAHINEKINDVLQGNLREIVSEMTVDQILT
                     NRKQMAISVVENARPDLAKMGLEVVTFNVQDVKDAIDAQGHNHGVIEAIGVEQEELVK
                     KRASIAKAEAERDVSCAKAAAAMAANEKEIEAQTAIAKRNNELNLAKSKLKAEADKAA
                     ADADAAGMIQTNLRAKEVKESEADAEIAKQKKMVDLAAQEAEVQQRKLDA"
     gene            1235..1885
                     /locus_tag="ACZPAX_09305"
     CDS             1235..1885
                     /locus_tag="ACZPAX_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008116292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR1114132.1"
                     /translation="MNNTPFTIAQMRPIRDGMTVSRPAHLGAVPVTWFSLGAGTTITP
                     ESYDCTTLYIAAEGAGCFVLGDDARPTPICPGELLVMPPKTLCGAAADTGMIYTEIIL
                     KKENFTMNSLIKAGHPTALKDLISYEEGSIANLDVVHADNMKFVLMAFDEGTGLTPHR
                     APGNAILTALEGKAIIGYEGRDYELNAGESFRFDKNGLHSVTPQGKFKMSLLLVLE"
     gene            2046..>2418
                     /locus_tag="ACZPAX_09310"
     CDS             2046..>2418
                     /locus_tag="ACZPAX_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005945638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR1114133.1"
                     /translation="MYVDWEYYKIFYYVAKYQNFTRAARVLGNNQPNITHTMNKLEDQ
                     LHCVLFIRSNRGVTLTPEGETLYARIASAAVQIQDAEAELSASATLEHGAISISATET
                     ALNICLSEQLRAFHAQYPGIRL"
BASE COUNT          501 a          710 c          671 g          536 t
ORIGIN      
        1 tccgcatcca gcttgcgctg ctgcacctcg gcctcctggg cggccaggtc gaccatcttt
       61 ttctgctttg caatttcggc gtccgcctcg gactccttga cctcctttgc acgcaagttt
      121 gtctggatca taccggcggc gtccgcgtcg gcagcggcct tgtcggcctc ggctttcagt
      181 ttggacttcg ccaggttcag ctcgttgttg cgcttggcaa tggcggtctg ggcttcgatt
      241 tccttttcgt tggctgccat ggcggcggca gccttcgcgc aagagacgtc gcgctcagct
      301 tcggccttgg cgatggacgc gcgcttcttg accagctcct cctgctcaac gccgatggcc
      361 tcgatgacgc cgtggttgtg gccctgggcg tcgatggcgt ccttgacgtc ctgcacgttg
      421 aaggtcacga cttccaggcc catcttggcc aggtcgggcc gggcgttctc gacgacgctg
      481 atggccatct gtttgcggtt ggtcaaaatc tggtcaacgg tcatctcgga gacgatttca
      541 cgcaggttgc cttgcagcac gtcgttgatc ttctcgttga tgtgggcctc atccatgcct
      601 aagaagttgg aaatcgccgc ctgctgacgg gagttgatgt actttttcac gtcgtcgtac
      661 ccggcgttgc ggacttcctc atccgcgaca gtggagttct cgctgtacac ctgcacggtc
      721 acgacgctgt ccagccagag ggagacgccc tccttggttt tgacaccggt ttccggcgtt
      781 tttacatcga ttttgagtaa gcgcatattc agccggtccg cccgctgcac gatgggcagc
      841 acaaacacgc cgcgcccgca gacgaccttg tgtcggcggg gcctttttgt agcagagaaa
      901 aaagaacaca atgagcgccg ccacggcagc ggcaataatg attacggtca acatatacca
      961 tatcctcctt tgtggttggt ggctttatta tagccgccgc tcacgccgca aaacaggacg
     1021 aaaacggcag aataagcgca gtattttgct gctggaggag gattttgttg agtgaagaaa
     1081 gtacgggcga gcattgctcg cccgtggtga ggcgggtaac tagggaaaac ctccatttaa
     1141 tcacggaacc cctaataatt tcttgtaatt gctgtttcta caacatcccc gccgcctcaa
     1201 atgtggtata ctattaaaaa aggagtgact tcccatgaac aacaccccct tcaccatcgc
     1261 gcagatgcgc cccatccgcg acggcatgac cgtctcgcgc cctgcccatt tgggcgcggt
     1321 gccggtgact tggttctcgc tgggcgcggg cacgactatc acgccagaaa gctacgactg
     1381 caccacgctc tatatcgcgg ccgagggcgc aggctgcttt gtgctgggcg acgatgcacg
     1441 ccccacgccg atatgccccg gtgaattgct cgttatgccg ccaaaaacat tgtgcggtgc
     1501 agcggcggac accggcatga tctacaccga aatcattttg aaaaaggaga attttactat
     1561 gaacagtctc atcaaagcgg gccatcccac ggccctgaaa gacctgattt cctacgagga
     1621 aggcagcatt gccaatctgg acgtcgtcca cgccgacaac atgaagtttg tgctgatggc
     1681 cttcgacgaa ggtaccggcc tgaccccgca ccgtgccccc ggcaacgcta tcttgaccgc
     1741 gctggaaggt aaggccatca tcggctacga gggccgggac tacgagctga acgccggcga
     1801 gagtttccgc tttgacaaga acggtctgca cagcgtcacg ccgcagggca agttcaagat
     1861 gagtctgctg ctggtgctgg aataatattt aacgataaag tccgctgccc cgcaaaaaca
     1921 aaacccctgc cgcccattgc atttgtggcg gactgctggt atactgggaa catacaagat
     1981 ataaaatatg tatcctgtat atagacgcca tgtaaataat acatgggaag agaggggccg
     2041 catccatgta cgtggattgg gaatactaca aaatttttta ttatgtcgcc aaatatcaga
     2101 actttaccag ggcggcgcgg gtgctgggca ataaccagcc taacatcacc cacaccatga
     2161 acaaactcga ggaccagctg cattgcgtgc tgttcatccg ttccaaccgg ggcgtgacgc
     2221 tgacgccgga aggggagacc ctttacgccc gcatcgcgtc ggccgccgtg cagatccagg
     2281 acgctgaggc cgaactgagc gccagcgcta cgctggaaca cggcgctatc agcatcagcg
     2341 ccaccgagac cgcgctgaac atctgcttgt ccgagcagct gcgggcgttc catgcgcagt
     2401 accccggtat ccggctgc
//
LOCUS       DBMDSQ010000307         2411 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_9660_length_2411_cov_1.992208, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000307 DBMDSQ010000000
VERSION     DBMDSQ010000307.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2411)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9660_length_2411_cov_1.992208"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..727
                     /locus_tag="ACZPAX_09315"
     CDS             <1..727
                     /locus_tag="ACZPAX_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019162279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HIR1114134.1"
                     /translation="TALQPADETATGAARFADVLVEGCYVAHVSRWGIAVGYTYAHAQ
                     FRGAELAEKTFLQYGHENVVLRDNYVKAAGGDGLTVMYALRPLVEHNTADSVACEMND
                     RVYREPGNRMGKVAAAIWPWKCKDALFRYNEAVDTRLNQDGMAYDADSGDGTVYEHNY
                     SRMNEGGCVMFCLEEAIHNTFRDNVSYDDLGGTISPSQNPDARIEENTFYVRNGVPFV
                     RPHMGGGSYTERDNTVIPLQEKE"
     gene            complement(817..1845)
                     /locus_tag="ACZPAX_09320"
     CDS             complement(817..1845)
                     /locus_tag="ACZPAX_09320"
                     /inference="COORDINATES: protein motif:HMM:NF024214.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HIR1114135.1"
                     /translation="MLLTGGRGSGKTTLLRALMPSLCPDAPMLLTAAVPGRWVEMRDT
                     AAGAAAVIGRFDAALPPGENRMRPVPAGFAAVGLPALQRMAAAGGWAVLDELGYLESG
                     CADFQQSVLDLLKVCRVLAVVRKQDTPFLRALCADPDAFVYDLDRPVPPLGCIVMASG
                     LGRRFGGNKLMAELNGRPLAAHALALAAAPVFAGRIAVTRSAEVEALCRAEGFPVLRH
                     AEPRRSDTVRLGLTALLAQQPDLQGCVFLPGDQPCLTRQTLEALAIGAAPDTIRRPAA
                     PDGTPGSPVLFGRDYFAALLHLPEGSGGSAVLRAHPQAIRLLPTPAAELRDIDTRSDL
                     EALRGGKG"
     gene            complement(1853..2194)
                     /locus_tag="ACZPAX_09325"
     CDS             complement(1853..2194)
                     /locus_tag="ACZPAX_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015553259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HIR1114136.1"
                     /translation="MKPALRVMLCDDTGERFFGEGPCRLLHLIEETGSLRSAAAQMGL
                     SYSKALRLVQHAEKELGFALTCKTIGGRGGGGSTLTAEARQFLERYEAYRDACVQTSR
                     ELYSAFFPGQR"
     gene            complement(2204..>2411)
                     /locus_tag="ACZPAX_09330"
     CDS             complement(2204..>2411)
                     /locus_tag="ACZPAX_09330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1114137.1"
                     /translation="VCLHDPALALDSCDILVVLQGGGVAAVLHPRTDPPAVLQAALAA
                     VYGPLELLPVTDCRGRRRLALLPL"
BASE COUNT          452 a          800 c          812 g          347 t
ORIGIN      
        1 gaccgccttg cagcccgcag atgagaccgc taccggcgcg gcacgctttg ccgatgtgct
       61 ggtcgagggc tgctatgtgg cccatgtcag ccgctggggg atcgcggtgg ggtataccta
      121 cgctcatgcg cagttccggg gggccgagct ggcagaaaaa acctttctgc agtacggcca
      181 cgagaatgtc gttctccgtg ataactacgt gaaagcggcg ggcggcgacg gcctgacggt
      241 catgtacgcc ctgcgcccgc tggtcgagca caacacggcg gactccgtcg cctgcgagat
      301 gaacgaccgt gtttatcggg agcctggcaa tcgcatgggc aaggtagctg ctgccatctg
      361 gccgtggaag tgcaaggatg cgttgttccg ctataacgaa gccgttgaca cgcggctcaa
      421 tcaggacggc atggcctacg atgccgactc cggcgacggc acggtgtatg agcacaacta
      481 cagccgcatg aacgagggcg gctgcgtgat gttctgtttg gaggaggcca tccacaacac
      541 tttccgtgac aacgtcagct atgacgacct cggcggtacg atcagcccct cccaaaaccc
      601 ggacgcacgg atagaggaga acaccttcta tgtccgcaac ggcgtcccct ttgtccgccc
      661 ccacatgggc ggcggcagct ataccgagcg ggacaacaca gtgatccccc tgcaggaaaa
      721 agaataagaa aaaagagcgc cccggatgct gagagcttca acatccgggg cgctcctttg
      781 tggaaaaacg gcgggcaata aatcaaaaat acggcattat cccttgcccc cgcgcagcgc
      841 ctcaaggtcg ctgcgggtgt cgatgtcccg cagctcggcg gcgggggtgg gcagcaggcg
      901 gatcgcctgc gggtgggcgc gcagcacggc gctgccgccg ctgccctccg gcaggtgaag
      961 gagggcagca aaataatccc gaccgaacag cacggggctg cccggtgtgc catcgggtgc
     1021 ggccggccgc cggatcgtgt ccggcgcggc accgatcgcc agcgcctcca gcgtctggcg
     1081 ggtcaggcag ggctggtccc ccggcagaaa cacacacccc tgcaggtcgg gctgctgcgc
     1141 cagcagcgcg gtcaggccaa ggcgcaccgt atcgctgcgg cgtggctcgg catggcgcag
     1201 cacggggaag ccctctgcac ggcagagggc ctcgacctcg gccgagcggg tcacggcaat
     1261 gcgcccggca aacaccggcg cggcggccag cgccagcgca tgggcagcca gcggccggcc
     1321 gttcagctcg gccatcagct tgttgccgcc gaaccgccgc cccagccccg aggccatgac
     1381 gatgcagccc agcggcggca caggacggtc cagatcgtac acaaaggcgt ccgggtcggc
     1441 gcagagcgcg cgcaggaacg gggtgtcctg cttgcggacg accgcgagaa cgcggcagac
     1501 tttcaacaga tccagcacgc tttgttgaaa atccgcgcag ccgctctcca gatagccaag
     1561 ctcgtccagc acggcccagc cgccggccgc cgccatccgc tgcagggcgg gcagccccac
     1621 ggcggcaaag ccggccggca cggggcgcat ccggttttca ccggggggca gggcggcatc
     1681 aaaccggccg atgaccgccg ctgccccggc ggctgtatcg cgcatctcga cccatctgcc
     1741 cgggacggca gcggtgagaa gcatcggggc atcggggcag agggatggca taagtgcgcg
     1801 cagcagcgtt gttttgccgc tgccgcgccc gccggtcagc agcaagtggc gcttaccgct
     1861 ggcccggaaa gaacgcagaa tagagctcgc ggctggtctg tacacaggca tcacggtagg
     1921 cctcatacct ttctaaaaat tggcgggcct cggcggtcag ggtgctgccg ccgccgcccc
     1981 gcccgccgat ggtcttgcag gtcagggcaa agcccagttc tttttcggcg tgctgcacaa
     2041 gccgcagcgc cttggagtag gaaagcccca tctgcgccgc tgcgctgcgc agcgagccgg
     2101 tctcctcgat caggtggagc aggcggcagg gcccctcgcc aaaaaagcgc tcgcctgtgt
     2161 catcgcagag catcacgcgc aaagcaggtt tcatggtatc acctcacagc ggcagcaggg
     2221 ccagacggcg tcggccccgg cagtcggtaa cgggcagcag ctccagcggg ccgtacacgg
     2281 cggcaagggc tgcctgcagc acggcaggcg ggtcggttct ggggtgcagc accgccgcaa
     2341 ccccgccgcc ctgcaatacc accagtatat cacagctgtc cagcgccagc gcggggtcgt
     2401 gcaggcagac c
//
LOCUS       DBMDSQ010000308         2404 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_9687_length_2404_cov_1.975684, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000308 DBMDSQ010000000
VERSION     DBMDSQ010000308.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 2404)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2404)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2404
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9687_length_2404_cov_1.975684"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..796
                     /locus_tag="ACZPAX_09335"
     CDS             <1..796
                     /locus_tag="ACZPAX_09335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1114138.1"
                     /translation="ITVYPGDIVPALPFGWPVVVKPADSPAWWNCHFPGKRKVYLARD
                     AAELQEILSAAARSSYRGAMLLQEYIPGPDTRLGVVNAYCAADGSVPWLVQGQPLLQE
                     RTPEGIGNYAAVLVEPARQDTALLEALRGLLQTAGWRGFANFDLKYDRRSEPVLFELN
                     PRQGRAAYYCDAAGAPLARPLVEDLLFGGPVTPPALRPAAWYTAPWGVVRRHCPNKLL
                     LRRAALLRRRGRGYPHLLAPGEGMARQIWFAARQAGYGRKLRKNGG"
     gene            786..>2404
                     /gene="asnB"
                     /locus_tag="ACZPAX_09340"
     CDS             786..>2404
                     /gene="asnB"
                     /locus_tag="ACZPAX_09340"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0070981 - L-asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HIR1114139.1"
                     /translation="MGAESMCGIVGFTTAAGHDPAAAKRTAQAMADLIRHRGPDGEGY
                     YADAHAALGHRRLSIIDLAGGGQPMFNEDGTLVVVFNGEIYNYKALHAELTARGHTFA
                     TDSDTEVLLHGYEAWGGDLPKHLRGMFAFALWDRTAETLFCARDLFGIKPLYYYKKGD
                     VLLFASEIKAFLAHPAFEKRLNEARLPDWLSMEYLPDDETMFLGVYELPPAHTLTWHD
                     GRLTTRRYAAPRFRARRGRSLGAWAEKIGQTLAESADAHRIADVAVGCFLSGGVDSSL
                     VTRETARSQPNVQCFSVGYAEESYSELPAARAAARALGVPLTETTVTAAEFFAANRAI
                     QWYLDEPMPNPAEVPLYFLCKTARQRVKVVLSGEGADELFGGYPLYRQAVWAERWQAL
                     PRSLRRGLAAALPGCGFLRRGAQPRWQRCARANYVFETAQERDKYLKRPSNTPSPARR
                     CKPYFDAVRGLDEPTALQWVDLQTWLPRDILRKADRMSMAHSLELRVPFLDRRVLAVA
                     LGLPRRYRCTGRRGKIALRAAAARRLPGALAA"
BASE COUNT          365 a          804 c          818 g          417 t
ORIGIN      
        1 cataacagta tacccggggg acattgtgcc cgcgctgccc ttcggctggc cggtcgtcgt
       61 caagcctgct gactcgcctg cctggtggaa ctgccatttc ccgggcaagc gcaaggtcta
      121 tctggcccgg gatgccgccg aattgcagga gattttgtcc gcagccgccc gcagcagcta
      181 ccgcggggcc atgcttttgc aggagtacat ccccggcccg gatacgcggc tgggggtcgt
      241 gaacgcctac tgcgccgccg acggcagtgt gccgtggctt gtgcaggggc agcccctttt
      301 gcaggagcgt acacccgagg gcattggcaa ctacgccgct gtgctggtgg aacccgcccg
      361 gcaggacacc gccttgctgg aggctctgcg cggccttttg cagacggcgg gttggcgcgg
      421 atttgccaat tttgatttga aatacgaccg ccgcagcgag ccggtgctgt ttgaactgaa
      481 cccccggcag ggccgcgcgg cttattactg cgatgctgcc ggtgccccgc tggcccggcc
      541 tttggtggag gatctgctgt tcggcggtcc ggtcacgccg cccgccctgc gcccggcggc
      601 gtggtacacg gccccctggg gcgtggtgcg ccgccattgc cccaacaagc tgctcctgcg
      661 ccgtgccgcc ctcctgcgac gccgcgggcg cgggtatccc catttattgg ccccggggga
      721 ggggatggca aggcagattt ggtttgcggc aaggcaggcg ggatacggca gaaaattacg
      781 aaagaatggg ggctgaatcc atgtgcggca tcgtcggctt taccaccgcc gccgggcatg
      841 acccggcggc ggccaaacgc acggcgcagg ctatggcgga cctcatccgc caccgcgggc
      901 cggacggcga gggctactac gccgacgccc acgccgcgct gggccatcgg cggctgtcca
      961 tcatcgacct tgcgggcggc ggccagccga tgttcaacga ggacggcacc cttgtcgtcg
     1021 tgttcaacgg cgaaatttac aactacaagg ccctgcacgc cgagctgacg gcccggggcc
     1081 atacctttgc taccgactcc gacaccgagg tgctgctcca cggctacgag gcctggggcg
     1141 gcgacctgcc caagcacctg cgcgggatgt tcgcctttgc cctgtgggac cgcacggccg
     1201 agacattgtt ctgcgcccgc gacctctttg gcatcaagcc gctgtactac tacaaaaagg
     1261 gcgatgtact gttgtttgcc agcgagataa aggcctttct ggcccacccg gcctttgaaa
     1321 agcggctaaa cgaagcccgc ctgcccgatt ggctcagcat ggagtacctg cccgacgatg
     1381 agacgatgtt tttgggtgtg tacgagctgc cgcccgccca cacgctgacc tggcatgatg
     1441 gccgtctgac cacccgccgc tatgccgcgc cgcgcttccg ggcgcggcgc ggccgcagcc
     1501 tgggggcctg ggcggaaaaa atcggccaga ctttggccga gagcgccgat gcccaccgca
     1561 ttgccgatgt ggccgtgggc tgctttttat ccggcggcgt cgattcgtcc ctcgtcaccc
     1621 gcgagacggc ccgcagccag cccaacgtgc agtgcttttc ggtcggctat gccgaggaaa
     1681 gctactccga gctgcccgct gcccgcgccg ccgccagggc actgggcgtg cccttgaccg
     1741 agacgaccgt gactgcggcg gagttcttcg cggcaaaccg ggccatccag tggtatctgg
     1801 atgaaccgat gcccaacccg gcggaagtgc cgctgtattt cctgtgcaaa accgcacggc
     1861 agcgtgtaaa agtggtgctt tcgggcgagg gcgcggatga actgttcggc gggtacccgc
     1921 tgtaccggca ggccgtctgg gccgagcgct ggcaggcgct gccccgcagc ctgcggcgcg
     1981 ggttggcggc ggccctgccg gggtgcggct ttctgcgccg gggtgcccag ccccggtggc
     2041 agcgctgcgc ccgggctaac tatgtgtttg aaactgcgca ggaacgcgac aaatatttga
     2101 agcgcccgtc caatacaccc agcccggccc ggcggtgcaa gccttatttt gacgcggtgc
     2161 gcggcctgga tgagccgaca gccctgcaat gggtggatct gcagacctgg ctgccccggg
     2221 atatcctgcg caaggccgac cgaatgagca tggcccacag cctggaactg cgggtgccgt
     2281 ttctggaccg gcgggtgctg gccgtggcgc tgggcctgcc ccggcggtac cgctgcaccg
     2341 ggcggcgcgg caagatcgcc ttgcgggctg cggcagctcg ccgcctgccc ggggcactgg
     2401 ccgc
//
LOCUS       DBMDSQ010000309         2400 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_9703_length_2400_cov_1.716398, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000309 DBMDSQ010000000
VERSION     DBMDSQ010000309.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2400)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9703_length_2400_cov_1.716398"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..>2400
                     /locus_tag="ACZPAX_09345"
     CDS             <1..>2400
                     /locus_tag="ACZPAX_09345"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015565625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HIR1114140.1"
                     /translation="IYQNWCDKGYFHTQIDRSKKPFTIVMPPPNVTGQLHMGHAMDET
                     WQDILTRYKRMQGYAALWVPGTDHASIATEAKVVAKMREEGLTKEMVGRDGFLERAWD
                     WKNTYGNRIVSQLKKLGCSCDWQRERFTMDEGCSNAVKEVFVRLYNEGLIYRGNRMVN
                     WCPHCNTSISDAEVEYEAKEGSFWHLLYPVKETGEMLELATTRPETMLGDTAVAINAE
                     DPRYKHLHGCHVVLPLLGREIPIVCDEHADMEKGTGVVKITPAHDPNDFEVGKRHDLP
                     MIRVLTYDGHMTGAADKAAVDAEKAAGRAAADEPDVLDCGKYAGMTALEARKAILADL
                     EACGALKETEPLTHDVGTCYRCHSVIEPMVSKQWFVKMEPLAKPAIESVEKGEIKFVP
                     ERFTKNYINWMKGGRDWCISRQLWWGHQIPAWYCDDCGETVVAKEAPCTCPKCGSTKL
                     TQDPDTLDTWFSSALWPFSTLGWPNENAEDYNYFYPTNTLVTGYDIIGFWVSRMIFSG
                     LAYTGKAPFDTVLIHGIVRDSQGRKMSKSLGNGIDPLEVIEQYGADALRMMLIIGSTA
                     GNDMRYSDEKVLACRNFANKLWNASRFVQMNLPEDFEPGLPAEDLLDMSDKWILSELA
                     KVAAEATANLDKYELGLAAEKVENFIWEVYCDWYIEICKTRLNGEDAAAADAARKVLV
                     YVLDKALKLLHPFMPFITEEIYQALPGSAETIMNEKWPGDENMKVWAEDCADFEKLMD
                     YIKAVRAMRAEMNVHPAKKTSMVIETASPAAFEKGGAYLARFAFATDVTVTAKYEGST
                     DG"
BASE COUNT          518 a          765 c          711 g          406 t
ORIGIN      
        1 atctaccaga actggtgcga caaggggtat ttccataccc agatcgaccg cagcaaaaaa
       61 ccgtttacca tcgtcatgcc gccgccgaac gttaccggcc agctgcacat gggccacgcc
      121 atggacgaaa cctggcagga catcctgacc cgctacaagc ggatgcaggg ctacgccgcc
      181 ctgtgggtgc ccggcaccga ccatgcctcc attgcgaccg aggccaaggt cgttgccaaa
      241 atgcgcgagg agggcctgac caaagagatg gtcggccgcg atggcttttt ggagcgcgcc
      301 tgggactgga agaacaccta cggcaaccgc atcgtcagcc agctgaaaaa gctgggctgc
      361 tcctgcgact ggcagcgcga gcgcttcacg atggacgagg gctgctccaa cgccgtcaag
      421 gaagtgttcg tccgcctgta caatgagggc ctgatctacc gcggaaaccg catggtcaac
      481 tggtgccccc actgcaacac ctctatctct gacgccgagg tcgagtacga ggccaaggag
      541 ggcagcttct ggcatctgct ctaccccgtc aaggagaccg gcgaaatgct ggagctggct
      601 accacccgcc ccgagacgat gctgggcgat actgccgttg ccatcaacgc cgaggacccg
      661 cgctacaagc acctgcacgg ctgccatgtg gtactgccgc tgctgggccg tgaaatcccc
      721 atcgtctgcg acgagcacgc cgacatggaa aagggcaccg gcgtcgtgaa gatcaccccc
      781 gcccacgacc ccaacgactt tgaggtcggc aagcgccacg acctgccgat gatccgcgtg
      841 ctgacctatg acggccacat gaccggtgct gccgacaagg ccgccgtcga tgccgagaag
      901 gctgcaggcc gcgctgccgc cgatgaaccg gacgtgctgg actgcggcaa atatgccggc
      961 atgaccgcgc tggaagcccg caaggccatc ctggctgacc tggaagcctg cggtgcgctg
     1021 aaagagaccg agccgctgac ccacgacgtg ggcacctgct accgctgcca ctctgtcatt
     1081 gagccgatgg tctccaaaca gtggttcgtc aagatggagc cgctggcaaa gcctgccatt
     1141 gagagcgtcg agaagggcga gatcaagttc gtgcccgagc gcttcaccaa gaactacatc
     1201 aactggatga agggcggccg cgactggtgc atcagccgcc agctgtggtg gggccaccag
     1261 atccccgcct ggtactgcga cgactgcggc gagaccgttg ttgccaaaga ggctccctgc
     1321 acctgcccca agtgcggcag caccaaactg acccaggacc ctgacacgct ggacacttgg
     1381 ttcagcagtg cgctgtggcc gttctccacc ctgggctggc ccaacgagaa cgccgaggat
     1441 tacaactact tctatcccac caacaccctc gtcaccggct acgacatcat cggcttctgg
     1501 gtttcccgta tgattttctc cggccttgcc tacaccggca aagcgccgtt tgacacggtg
     1561 ctcatccacg gcattgtccg tgacagccag ggccgcaaga tgtctaaatc tctgggcaac
     1621 ggcattgacc cgctggaagt cattgaacag tacggcgcgg acgctttgcg catgatgctg
     1681 atcatcggct ccaccgccgg taatgatatg cgttacagcg acgaaaaggt actcgcctgc
     1741 cgcaactttg ccaacaagct gtggaacgct tcccgttttg tgcagatgaa cctgcccgag
     1801 gactttgagc cgggcctgcc tgccgaagac ctgctggaca tgagcgacaa gtggattttg
     1861 agtgagcttg ccaaggttgc cgccgaggcc accgcaaacc tcgacaagta cgagctgggc
     1921 ctggccgccg agaaggtcga gaacttcatc tgggaagtct actgcgactg gtatattgag
     1981 atctgcaaga cccgcctgaa cggcgaggac gcagctgccg ccgacgccgc ccgcaaggtg
     2041 ctcgtctatg tgctggacaa ggcgctgaag ctgctgcacc ccttcatgcc gttcattacc
     2101 gaggaaatct atcaggccct gcctggcagc gccgagacca tcatgaacga gaagtggccc
     2161 ggagacgaga acatgaaggt ttgggccgag gactgcgccg attttgagaa actgatggac
     2221 tacatcaagg cggtccgcgc catgcgcgcc gagatgaacg tccaccccgc caaaaagacg
     2281 agcatggtca ttgagacagc cagccccgcc gcattcgaga agggcggcgc gtatctggcc
     2341 cgtttcgcct ttgccacaga cgtgacggtc actgccaagt acgagggcag caccgacggc
//
LOCUS       DBMDSQ010000310         2400 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_9704_length_2400_cov_1.551544, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000310 DBMDSQ010000000
VERSION     DBMDSQ010000310.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2400)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9704_length_2400_cov_1.551544"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="ACZPAX_09350"
     CDS             <1..336
                     /locus_tag="ACZPAX_09350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1114141.1"
                     /translation="TDFLTQSGQELEETTGTVKYDFGFTVPDAAVEAVRYDCGSYQTI
                     FAQIDYDCGETVYVTAAARTSDYEPILDLLQDVWPTGHAPENAHTADTTTNAVTEEDV
                     NGGRSKSLA"
     gene            complement(442..1062)
                     /locus_tag="ACZPAX_09355"
     CDS             complement(442..1062)
                     /locus_tag="ACZPAX_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004458734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HIR1114142.1"
                     /translation="MNVLLINGSPHKNGCTNAALAEVAKTLNEAGIETTIFHIGAAPV
                     GGCVSCGGCGKAGKCVFGGPVADVLPLVEKADGIVFGAPVHYATAAASMLGFMHRLAI
                     SGGKYLRHKPAAVVTSARRAGTTTALEAMEKVPQFFEMPLISSTYWPMVHGGKAEDVL
                     SDEEGLQIMRNLGRNMAWMLKCIEAGKAAGIMAPTAENDKRTNFIR"
     gene            1238..>2400
                     /gene="rlmD"
                     /locus_tag="ACZPAX_09360"
     CDS             1238..>2400
                     /gene="rlmD"
                     /locus_tag="ACZPAX_09360"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005923076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000154 - rRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HIR1114143.1"
                     /translation="MTTTCLNFTKKCGGCPLLAMPYREQLAQKQARLQELLGGFAPVK
                     AVQGMADPLHYRNKAIASFAAQHGKLVCGLYAEGTHKVLPGADCLLQEDILNKTLAAV
                     LDAARTCRWTAYDEDRGTGLLRHTVLRSSADGKVLVTLVTPGPELPGSKNFCAAMRKK
                     APWVVSIVQNINPTRTSAVLGNREKTLYGPGFVRDTLCGTQFAISSRSFYQVNRTQTE
                     VLYKKALELAKLTGRETVVDAYCGIGTIGLCAAPQAKQVIGVERNPAAVKDAAANARR
                     NKIANAHFVCADATEWMTAAAGEGLRPDVVFLDPPRAGSTPECIAAVNKMKPRRVVYV
                     SCDPETLARDVAAFTRLGWRAAKFCPVDLFPQTRHVETVVLLSKGEVDSKKIRV"
BASE COUNT          476 a          775 c          701 g          448 t
ORIGIN      
        1 acggactttc tgacccagtc cggccaggaa ctggaagaaa ccaccggcac ggtcaagtat
       61 gattttggtt ttactgtgcc cgatgccgct gtggaggctg tgcgctacga ctgcggcagc
      121 tatcagacga tttttgcgca gatcgactac gactgcggtg agacggtcta tgtgaccgct
      181 gctgcccgca ccagcgacta tgagccgatt ctggaccttt tgcaggatgt ctggcccacc
      241 ggccatgcgc cggaaaacgc ccacacggcc gacaccacga ccaacgccgt gaccgaggag
      301 gacgtgaacg gcgggagaag caaaagcttg gcgtaaaggc aaagaggcaa agcccgcgaa
      361 aaaagcctcc cctcacaggg aaggcggtcg ggcgatagcg atgaccggaa gggctttgcc
      421 gccttcccgt aagaaccaac tttaccttat aaaattcgtc cgcttatcat tctccgccgt
      481 cggggccatg atccccgcag ccttgccagc ctcgatgcac ttgagcatcc acgccatgtt
      541 gcggcccagg ttgcgcatga tctgcaagcc ttcctcgtcg gagagcacgt cctcggcctt
      601 gccgccatgg accatcggcc agtaggttga gctgatgagc ggcatctcaa agaactgcgg
      661 taccttctcc atcgcttcca gtgcggtggt cgtaccagcg cggcgggcac tggtgacgac
      721 tgcggcaggc ttgtggcgca ggtacttccc gccgctgatg gccagccggt gcataaagcc
      781 cagcatactg gccgccgccg tggcgtaatg taccggtgcg ccaaacacga tgccgtcagc
      841 cttttccacc agcggcagca cgtcggccac cggcccgcca aagacgcatt tgcccgcctt
      901 gccgcagccg ccgcagctta cacagccgcc caccggggcc gcgccgatgt ggaagatcgt
      961 cgtttcgatg cccgcttcgt tcagcgtctt ggccacctcg gccagtgctg cgttggtgca
     1021 gccgtttttg tgcgggctgc cgttgatcag tagtacgttc atagtcaata cccccgtttt
     1081 tgatttgctc tgtataccat tatagcatag tacgcaagca tttgtgtagg agcggcatat
     1141 aggccgcccg cggttttccg gcagacgacc acttgcgggc cgcatatatg ccgcccctgc
     1201 tatgtttaaa cgaatcacta aagaaaggtc catccccatg accactacct gcctgaattt
     1261 taccaaaaaa tgcggtggat gtccgctgct ggccatgccc taccgggaac aactggccca
     1321 aaagcaggcc cgcctgcaag aactgctggg cggctttgcg ccggtcaagg ccgtgcaggg
     1381 catggccgac ccgctgcatt atcgcaacaa ggctatcgcc agctttgctg cccagcacgg
     1441 caagctcgtc tgcggcctgt acgccgaggg cacgcacaag gtcctgcccg gtgcggactg
     1501 cctgctgcag gaggatattt tgaacaaaac gctggccgct gtgctggacg ccgcacgaac
     1561 ctgccgctgg accgcctacg acgaggaccg cggcaccggg cttttgcgcc atacggtgct
     1621 gcgcagttcc gctgacggca aagtgcttgt cacactggtc acgcctggcc ccgagctgcc
     1681 cggcagcaag aatttttgcg ccgcgatgcg aaaaaaagcg ccgtgggtcg ttagcatcgt
     1741 gcagaacatc aaccctaccc gcaccagcgc tgtgctgggc aatcgcgaaa aaacgctcta
     1801 cggccccggc tttgtgcggg atacgctgtg cggcacgcag tttgcgattt cctcccgcag
     1861 cttttaccag gtcaaccgca cacaaacgga agttctgtac aaaaaggcgt tggaactcgc
     1921 caaactgacc ggccgggaga cagtcgttga tgcctactgc ggcatcggaa cgatcggcct
     1981 gtgcgctgcg ccgcaggcaa aacaggtcat cggcgtcgag cgcaatcccg ccgccgtgaa
     2041 ggacgccgcc gccaatgccc gccgcaacaa aatcgccaac gcccactttg tctgcgccga
     2101 tgccaccgaa tggatgacgg ccgccgcagg cgagggcctg cgcccggatg tggtgttcct
     2161 cgacccgccc cgcgcgggca gcacaccgga gtgtatcgcc gccgtgaaca agatgaagcc
     2221 gcgccgtgtc gtctacgtca gctgcgaccc cgagaccctg gcccgtgacg tcgccgcctt
     2281 cacccgcctg ggctggcgcg ccgccaaatt ctgcccggta gacctcttcc cacagacacg
     2341 gcatgtggag acggttgttt tgctttccaa gggtgaggtc gactcgaaaa agattcgggt
//
LOCUS       DBMDSQ010000311         2398 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_9715_length_2398_cov_2.109708, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000311 DBMDSQ010000000
VERSION     DBMDSQ010000311.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2398)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9715_length_2398_cov_2.109708"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..389
                     /locus_tag="ACZPAX_09365"
     CDS             <1..389
                     /locus_tag="ACZPAX_09365"
                     /inference="COORDINATES: protein motif:HMM:NF013700.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HIR1114144.1"
                     /translation="PGIWDAKPLAAFSGDELVYSATLGDWRTHNGADYAAPAGESVYP
                     AKAGKVDSVTEDALWGYAVEITDKNGVTWRYCGLTDTEVKAGDSVTTATSIGKAGSVP
                     AETSGEAHLHLECIKDGAYLDPEGLL"
     gene            complement(477..2339)
                     /locus_tag="ACZPAX_09370"
     CDS             complement(477..2339)
                     /locus_tag="ACZPAX_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HIR1114145.1"
                     /translation="MTKKQKKTLKRIIIAAVLTAVLALLFHFAELPWFVQLVLWLVPY
                     LVIGHDVLRKAFMGIKSGEVFDENFLMAVATVGAMGCGEYAEGVAVMLFYQIGELFQS
                     YAVGKSRSSISALMDIRPDSANLEAEDGTVSVVDPDDVPIGSTIVVKPGEKIPLDGVV
                     LSGESTLNTAALTGESRPRTVRPGDEVISGCVNDDSVLRVRTTKIYGESTVAKILDLV
                     ENSSLKKSPVENFITKFARYYTPAVCIGALVLAIVPPLFLGGWLDWIMRALTFLVISC
                     PCALVISIPLTFFGGIGGASKCGILVKGGNYLEALSKTGVAVFDKTGTLTKGVFKVTH
                     TAPADGVSEKDLLESAALAESFSSHPISKSLREACPDLDARRASDAQEIAGHGVKTQV
                     DGHTVLAGNARLMESEGVTYTAAEGAGTIVYVARDGQFLGSILISDEEKPTAKAAMQG
                     LQAQGVRTVMLTGDTPAVAELVAKDLGIDEVHAGLLPADKVEWVEKLLGQKPAKKELA
                     FVGDGINDAPVLMRADIGIAMGALGSDAAIEAADVVLMDDDPAKISLAMRISRKCMTI
                     AYQNIVFALAIKALCLILTALGITNMWWGVFADVGVMVLAVLNATRMLNVKQYQ"
BASE COUNT          476 a          743 c          752 g          427 t
ORIGIN      
        1 cccctggcat ctgggacgcc aagccgctgg cagcattcag cggggatgaa ctggtgtaca
       61 gtgcgacgct gggcgactgg cgcacccaca acggcgcgga ctacgctgcc ccggcagggg
      121 agagtgtgta cccggccaag gcgggcaagg ttgattccgt aaccgaggac gcactgtggg
      181 ggtatgcggt ggaaatcacc gacaagaacg gtgtgacctg gcggtactgc gggctgactg
      241 acacggaagt caaagccggg gacagcgtga cgaccgccac ctccatcggc aaggcgggga
      301 gcgtccccgc cgaaaccagc ggcgaggcgc atttgcacct ggagtgcatc aaggatggtg
      361 cgtacctcga cccggagggg ctgctgtaat agcagacggc aaaagccgtt ccccactgcc
      421 cgaaacccaa acaggcgtgt acccacatcg ggcacacgcc tgttcttatg caaattttac
      481 tggtactgct tcacattcag cattctcgtc gcgttcagca cggccagcac catgacgccg
      541 acgtcggcaa aaacgcccca ccacatattg gtaatgccca gcgcggtcag aatcaggcac
      601 agcgccttga ttgccagcgc gaacacaatg ttctggtagg caatcgtcat gcacttgcgg
      661 gaaatccgca tggcaaggct gatttttgcc gggtcgtcgt ccatcagcac aacatcggcg
      721 gcctcgatgg cggcatcact gcccagcgca cccatggcaa tgccgatatc ggcacgcatc
      781 agcaccggcg cgtcgttgat gccgtctccc acgaaggcaa gctctttctt tgcgggtttc
      841 tggcccagca gcttctcgac ccactcgacc ttgtcggcgg gcagcaggcc cgcgtggacc
      901 tcatcgatgc ccaaatcttt cgcaaccaac tcagccaccg caggggtgtc gccggtcagc
      961 atgacggtac gcacgccctg ggcctgcagg ccctgcatgg cagccttggc cgtgggcttt
     1021 tcctcgtcgg aaatcaggat ggagccgagg aactgtccgt cgcgggcgac gtagacgatg
     1081 gtgcctgcgc cctctgcggc ggtgtaggtg acgccctcgc tctccatcag gcgggcgttg
     1141 ccggccagca cggtatgacc atcgacctgc gttttcacgc cgtgacccgc gatttcctgc
     1201 gcgtcagagg cgcggcgggc atccaggtcg gggcaggcct cgcgaaggct cttgctgatg
     1261 ggatggctcg aaaaactctc ggccagcgcg gcactctcga gcagatcctt ctcgctcacg
     1321 ccgtcagcag gggcggtgtg ggtgactttg aaaacgccct tcgtcagggt gccggtcttg
     1381 tcaaacacgg caacgccggt cttggacagg gcctcgaggt agttgccgcc cttgaccagg
     1441 atgccgcact tggacgcgcc gccgatgccg ccgaagaagg tgagggggat agaaatgacc
     1501 agcgcacagg ggcagctgat gaccaggaag gtcagcgcac gcatgatcca gtcaagccag
     1561 ccgcccagga acagcggcgg cacaatggcc agcaccagtg cgccgatgca gacggcgggg
     1621 gtgtagtagc gggcaaactt cgtgatgaag ttctcgacgg gggacttttt caggctggaa
     1681 ttttccacca ggtcgagaat tttcgcaacg gtggactcgc cgtaaatttt tgtcgtccgg
     1741 acgcgcagca cactgtcgtc gttcacgcag ccgctgatga cctcatcgcc ggggcgcacg
     1801 gtgcgcgggc ggctctcgcc ggtcagggcg gcggtgttca gcgtggactc accggagagg
     1861 accacgccgt ccagcgggat cttttcgccg ggcttgacga cgatggtgga accgatgggt
     1921 acatcgtcag ggtcaacgac ggaaaccgta ccgtcctcgg cctccaaatt cgcgctgtcg
     1981 gggcgaatat ccatcagcgc cgagatgctg gaacggctct tgccgacggc gtaggactgg
     2041 aacagctcgc cgatctggta gaacagcata acggccacgc cctcggcata ctcgccgcag
     2101 cccatggcac caacggtggc gaccgccatc aggaagttct cgtcaaagac ctcgccgctc
     2161 ttgatgccca tgaacgcctt gcgcaggaca tcgtggccga tgaccagata cggcaccagc
     2221 cacagcacca gctgcacgaa ccagggcagc tccgcaaagt ggaacagcag cgccagcaca
     2281 gccgtgagca ctgccgcgat gataatgcgc ttcagtgtct ttttttgctt cttcgtcatg
     2341 gatatgacct ccattttggg tataaaaagg gcccatcgtc gggatgggcc gctttatg
//
LOCUS       DBMDSQ010000312         2379 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_9821_length_2379_cov_1.810799, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000312 DBMDSQ010000000
VERSION     DBMDSQ010000312.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 2379)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2379)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2379
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9821_length_2379_cov_1.810799"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1393)
                     /gene="pknB"
                     /locus_tag="ACZPAX_09375"
     CDS             complement(<1..1393)
                     /gene="pknB"
                     /locus_tag="ACZPAX_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HIR1114146.1"
                     /translation="MDNLIGKRLDGRYSIEGLVGVGGMANVYRGTDLKTGNRIAVKVL
                     KEEFLDNEELVRRFKNESKAISILSHPNIVKVYDVSVTDKLQYIVMEYVDGITLKEYL
                     KQRGGALTWKEVVHFATQVLSALQHAHSKGIIHRDVKPQNIMLLADGSIKMMDFGIAR
                     FSRAQSQTVSDKAIGSVHYISPEQAKGERTDARTDIYSVGVMLYEMLSGRLPFDGDGA
                     VSIAIMQISEKPKPLAEIAPNTPAGLRQITEKAMEKDPDKRYQSAQEMLDAIEEFKRN
                     PSIRFEYEYRNAEDNPERNINRVVNSAKPGAKAANIRTGDARRVGTANPGRSKSGKKK
                     KKASKGFSLFPIFFGMAVAFVIGAAILIYLIFTNSSNLLFSNRADVQIISFVGMTKDE
                     FLATDYNSLLRAEFPEEYSSEPAGTIIRQTPKAGRTVKEKQRIVLTVSLGTQYVTIPE
                     TKNMVAEDAEQTLK"
     gene            complement(1386..2135)
                     /locus_tag="ACZPAX_09380"
     CDS             complement(1386..2135)
                     /locus_tag="ACZPAX_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HIR1114147.1"
                     /translation="MKIAAITDIGSCRQENQDNYCAQQLAGGTAWGIVCDGMGGVNGG
                     RIAARIATDTMQQYFARHMKALQPGMEKTFIMRGFDITNRAVYEKSTSDPEMQGMGTT
                     GVCAYASRGLAHVVHAGDSRAYLFHAGALRQITRDHSMVQQLVDSGQITREQAAVHPQ
                     KNLITRALGVSANIVPEYNRCEIEDGDILLLCSDGLTNMVSDEDIAQVLREVPFFDAT
                     SILVDRALQAGGQDNITVLLMGVETTEVNNG"
     gene            complement(2171..>2379)
                     /locus_tag="ACZPAX_09385"
     CDS             complement(2171..>2379)
                     /locus_tag="ACZPAX_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005932700.1"
                     /note="23S rRNA m2A2503 methyltransferase; methylates the
                     C2 position of the A2530 nucleotide in 23S rRNA; may be
                     involved in antibiotic resistance; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dual-specificity RNA methyltransferase RlmN"
                     /protein_id="HIR1114148.1"
                     /translation="IKGMGAHVNLIPINPVDGSPYSATDDANVHRFQKELEALGVNAT
                     VRRRLGTDISAACGQLRREDAQGK"
BASE COUNT          432 a          675 c          724 g          548 t
ORIGIN      
        1 ccttcaaggt ctgctcggca tcctcggcga ccatgttctt tgtctcgggg atcgtgacat
       61 actgggtgcc caggctgacc gtcagcacga tgcgctgctt ttccttcacg gtacgcccgg
      121 ccttgggagt ctggcggatg atggtgcccg ccggttcgct ggaatattcc tccgggaact
      181 cggcacgcag caggctgttg tagtcggtgg caaggaactc gtccttcgtc atgcccacaa
      241 agctgatgat ctgcacgtcg gcacggttgg agaacagcag gttgctggaa ttggtgaaaa
      301 taagataaat cagaatggcc gcaccgatga cgaacgccac ggccatgccg aagaaaatcg
      361 ggaaaagcga gaagcccttg gaggctttct tcttcttttt gccggacttg ctgcggcccg
      421 ggttagcggt gcccactcgg cgggcgtcgc cggtgcggat gttggcggct tttgcgccgg
      481 gcttggcact gtttaccacg cggttgatgt tcctttcagg gttatcttcg gcattgcggt
      541 actcatactc aaagcggatg gagggattgc gcttgaactc ctcaatggcg tccagcattt
      601 cctgggcact ctggtagcgc ttgtcaggat ccttttccat ggccttttcg gtgatctggc
      661 gcagaccggc cggggtgttg ggggcaattt ccgccagcgg cttcggcttc tcggaaatct
      721 gcatgatggc aatggacact gcgccgtcgc cgtcaaaggg caggcggccg gacagcatct
      781 catacagcat gacaccgacg gagtagatgt cggtgcgggc gtcggtgcgc tcacccttgg
      841 cctgctcggg gctgatataa tgcactgagc cgatggcttt gtcgctgacg gtctggctct
      901 gtgcgcggga gaagcgcgca atgccgaagt ccatcatctt gatggagccg tccgccagca
      961 gcatgatgtt ctgcggcttg acgtcgcggt ggatgatgcc cttggagtgg gcgtgctgca
     1021 aggcgctcaa gacctgggtg gcaaagtgta cgacctcttt ccaggtcagg gcaccgccgc
     1081 gctgcttgag atattctttc agcgtgatgc cgtccacata ctccatgacg atgtactgca
     1141 atttgtcggt gacagataca tcatagacct tgacgatatt ggggtgactg aggatggaga
     1201 tggccttgga ctcgttcttg aaacggcgca ccagttcctc attgtcaaga aattcttcct
     1261 ttaaaacttt gacggcgatc cggttgccgg ttttcagatc ggtgccgcgg taaacattgg
     1321 ccatgccgcc aacgcccacc aggccttcga tcgaataccg gccgtccagc cgcttgccaa
     1381 tcagattatc cattgttgac ctccgtagtt tccacgccca tgagcagaac cgtaatgtta
     1441 tcctgaccgc ccgcctgcag ggcgcggtct accagaatgc tcgttgcatc aaagaaaggt
     1501 acctcacgca aaacctgcgc gatatcttca tccgacacca tgttggtcaa gccgtcggaa
     1561 cacaagagaa gaatgtcgcc atcctcaatc tcgcagcggt tgtactccgg cacgatgttg
     1621 gccgagacac ccagtgcgcg ggtgatgagg tttttctgcg ggtggacggc cgcctgttcc
     1681 cgggtgatct ggccgctgtc caccagctgc tgcaccatcg agtggtcgcg ggtgatctgc
     1741 cgcagtgccc ctgcgtggaa caggtatgcg cgggaatccc cggcgtgtac cacatgggcc
     1801 agtccgcggc tggcgtaggc gcagacaccg gtggtgccca tgccctgcat ttccgggtca
     1861 gaggtggact tttcatacac ggcgcggttc gtgatatcaa acccgcgcat gatgaaggtc
     1921 ttttccatgc ccggctgcag ggctttcatg tggcgggcaa aatactgctg catggtgtcg
     1981 gtggcaatac gtgcggcaat gcgcccgccg ttgacaccgc ccataccgtc gcagacgatg
     2041 ccccaggcgg tgccgcctgc cagctgctgc gcacagtagt tgtcctgatt ttcctgccgg
     2101 cagctgccga tgtccgtgat cgcggcaatc ttcattcagg ttggttcctt tccgttgcta
     2161 aaagcaagac ttattttccc tgtgcatcct cgcggcgcag ctggccgcag gcggcactga
     2221 tatcggtgcc caagcggcgg cgcaccgtgg cgttgacgcc gagcgcttcc agttctttct
     2281 ggaaacggtg gacattggcg tcatccgtgg cagaataagg gctgccgtcc accgggttga
     2341 tggggataag gttgacgtgt gcgcccatgc ccttgataa
//
LOCUS       DBMDSQ010000313         2360 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_9922_length_2360_cov_1.702966, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000313 DBMDSQ010000000
VERSION     DBMDSQ010000313.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 2360)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2360)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2360
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9922_length_2360_cov_1.702966"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            49..354
                     /locus_tag="ACZPAX_09390"
     CDS             49..354
                     /locus_tag="ACZPAX_09390"
                     /inference="COORDINATES: protein motif:HMM:NF014092.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HIR1114149.1"
                     /translation="MRFFLISDNSDAMNGLRLAGIEGTVARSEADLRHALADAANQPD
                     IAVLLVTEKIAETYTATLDSARAAGGPLLITVPSTGGGAAGKDRLTRYIREAIGVKI"
     gene            366..956
                     /locus_tag="ACZPAX_09395"
     CDS             366..956
                     /locus_tag="ACZPAX_09395"
                     /inference="COORDINATES: protein motif:HMM:NF014093.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033178 - proton-transporting two-sector
                     ATPase complex, catalytic domain [Evidence IEA];
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E family protein"
                     /protein_id="HIR1114150.1"
                     /translation="MNELDTRAERFLESIRAEGEAACAAIHEETEREISSQLDATRKA
                     ENARVERTLHFETERARTRANRDLSAARMAARATLADQRQKIADETFAEARSQLAAFA
                     AGDKYAAWLQNEAAALAEALGENANIAARPADLPLLQNVKLPVGTTLTADESIELGGL
                     KGANAAKGLVADDTLAARLNAQHEWFLQNAGLEINI"
     gene            968..>2360
                     /locus_tag="ACZPAX_09400"
     CDS             968..>2360
                     /locus_tag="ACZPAX_09400"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006356005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HIR1114151.1"
                     /translation="MENKIYSINGPVVTIKGKTDLEMMEMVYVGNARLVGEVIRMNDK
                     ETTIQVYEETGGLKAGEPVESTGGPLSVTLGPGILRNIYDGIQRPLPAIEQHMGSFIE
                     RGAHEPSLDESAKWDVTVTVKAGDTLTPGQIYATCPETPAIQHRCMVPPTISGTVTWV
                     AANGSYTVHSPIVKLTDAKGKEHTLTLCQKWPIRTPRPSAERMTSPRPLITGQRVIDT
                     MFPLAKGGAAAIPGGFGTGKTMTQHQIAKWCDADIIIYVGCGERGNEMTQALQEFSEL
                     VDPRTGKSLMDRTVLIANTSNMPVAAREASIYTGITLAEYYRDMGYHAAMMADSTSRW
                     AEALREISGRLEEMPADEGYPAYLPSRLAEFYERAGYVKTLNGAEGSVSVIGAVSPQG
                     NDFSEPVTQNTKRFTRCFWALDKSLAYARHYPAINWNDSYSEYLGDLGGWYFPSLKEL
                     ALLCAEDSWEKPLP"
BASE COUNT          480 a          785 c          706 g          389 t
ORIGIN      
        1 acggcctggt tatctccatc atgatcctgt ctaagctggt gtaatgctat gcggttcttt
       61 ctcatcagcg acaactccga cgccatgaac ggcctgcgtc tggcgggcat tgagggcacc
      121 gtagcccgca gcgaggccga cctgcggcac gcgctggcgg atgccgccaa ccagccggac
      181 attgccgtgc tgctcgtcac cgagaagatc gccgagactt acacagccac gctggacagt
      241 gcccgcgctg ccggtgggcc gctgctcatc accgtgcctt ccaccggcgg cggtgctgcc
      301 ggtaaggacc gcctgacccg ctatatccgc gaggccatcg gcgtgaagat ttgagaggtg
      361 tgaccatgaa cgaattagat acccgcgccg agcgctttct ggagtccatc cgcgcggaag
      421 gcgaggccgc ctgcgccgcc atccacgagg agaccgagcg ggaaatttcc agccagctgg
      481 acgctacccg caaggccgaa aacgcccgtg tggagcgcac cctgcacttt gagaccgagc
      541 gtgcccgtac ccgcgctaac cgcgatttga gcgctgcccg catggccgcc cgcgccacgc
      601 tggctgacca gcgccagaag attgccgacg agacctttgc cgaggcacgc agccagctgg
      661 ccgcctttgc cgccggcgac aaatacgccg cctggctgca aaacgaggct gccgcgctgg
      721 cggaagccct gggcgagaat gcgaatatcg ccgcccgccc ggcggatctg ccgctgctgc
      781 aaaacgtcaa gctgcccgtc ggcacgacac ttaccgccga tgaaagcatt gaactcggcg
      841 gtctgaaagg cgcaaacgcc gccaaaggct tggttgccga cgacacgctg gcagcacgcc
      901 tgaacgccca gcacgaatgg ttcttgcaga acgccgggct ggaaatcaac atttgagggg
      961 caaagccatg gaaaataaaa tttacagcat caacggcccc gttgtcacca tcaagggcaa
     1021 aaccgacctg gaaatgatgg aaatggtcta tgtgggcaac gcccgccttg tgggcgaggt
     1081 catccgcatg aacgacaagg aaaccacgat ccaggtatat gaggagaccg gcggcctgaa
     1141 agcaggcgag cctgtcgaga gcacgggcgg cccgctgtcc gtcaccctcg gccctggcat
     1201 cctgcgcaac atctatgacg gtatccagcg cccgctgccc gccattgagc agcacatggg
     1261 cagctttatc gagcgcggtg cccatgagcc gtccctggac gagagcgcca agtgggacgt
     1321 gaccgtgacc gtcaaggcag gggacaccct gacccccggc caaatctacg ctacctgccc
     1381 ggagacaccc gccatccagc accgctgtat ggtaccgccg acgatttccg gcaccgtgac
     1441 ctgggtcgcc gctaacggca gctacaccgt ccattcaccc attgtaaagc tgaccgacgc
     1501 caagggcaag gaacacacgc tgaccttgtg tcagaagtgg cccatccgca ccccgcgtcc
     1561 gtcggccgag cgcatgacct cgccccggcc gctgattacc ggccagcgcg tcattgatac
     1621 gatgttcccg ctggccaagg gcggtgcggc ggccatcccc ggcggcttcg gcaccggcaa
     1681 aacgatgacc cagcaccaga tcgcaaagtg gtgcgacgcc gatatcatca tctatgtcgg
     1741 ctgcggcgag cgcggcaacg agatgaccca ggcgctgcag gagttcagcg aactggtcga
     1801 cccccgcacg ggcaagagcc tgatggatcg taccgtgctg attgccaaca cgtccaatat
     1861 gcctgttgct gcgcgtgagg cgtccatcta caccggcatt acactggctg agtattaccg
     1921 cgacatgggc taccacgccg ccatgatggc tgactccacc tcccgctggg ccgaggctct
     1981 gcgtgagatc agcggccgtc tggaggaaat gcccgccgat gagggctatc ctgcgtacct
     2041 gcccagccgt ctggcggagt tctacgagcg cgccggttat gtcaagacgc tgaacggcgc
     2101 agaaggttcc gtctcggtca tcggtgcagt cagcccgcag ggcaacgact tctccgagcc
     2161 tgtcacccag aacaccaagc gcttcactcg ctgcttctgg gcgctggata agtccttggc
     2221 ttatgcccgc cactacccgg ccatcaactg gaacgactcc tacagcgagt atttggggga
     2281 cctcggcggc tggtatttcc cgtctctgaa agaactggca ctactctgcg ccgaggattc
     2341 atgggaaaag ccgctacccg
//
LOCUS       DBMDSQ010000314         2359 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_9926_length_2359_cov_1.596988, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000314 DBMDSQ010000000
VERSION     DBMDSQ010000314.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 2359)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2359)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2359
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9926_length_2359_cov_1.596988"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(89..1900)
                     /locus_tag="ACZPAX_09405"
     CDS             complement(89..1900)
                     /locus_tag="ACZPAX_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007048667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HIR1114152.1"
                     /translation="MTIFNVFTLMGGIAMFLYGMDLMGKALEQTAGSKLQGILSTMTS
                     SPIRALLLGVAVTAVIQSSGATTVMAVGFVNSGLMELHQAIGVIMGANIGTTVTGWLL
                     SLSGLEGESFITKMLNPNAWSPILGFIGIWLYMVGKDRKRGVGKILLGFAILMAGMNT
                     MSNAMSPLADEPWFMNLFLSFSNPVLGVIAGAVLTAVLQSSSAAVGILQALTSTGAVT
                     YSAAVPIIMGQNIGTTVTALISSAGANKNAKRTAFVHLYFNVIGTIIFLCGFYGLHSL
                     ICFSFFNATANTFGIAMVHTVFNIVTTAILLPFNRLLEKLAIMTVPDSPADKKQENSL
                     LDDRLLATPSVAVARAMLTGGDMAEICRTSLLQAMSTTHKWNDAIADEVERKEDAVDH
                     YEDVLGTYLVKLSAKHLSTEDNRTINTLLHTIGDFERVSDHAVNLIKTAEEIRDKSIQ
                     FSEEALGDLGVLEAAVQDIVNRTVDAFQKGDVYAAGKIEPLEQVVDGLVREVKTRHIA
                     RLQAGVCTIEYGFVLDDLLTNYERIADHCSNIAVAMIEVAADKFDTHEYLASVKHGGS
                     VKFEQRYEKYRGRYTFPPETYAEMQPVSAPGNAEEDA"
BASE COUNT          501 a          648 c          704 g          506 t
ORIGIN      
        1 tgctggcgtc atcctccaca atgtagatca tataagttcc tctctcttac aaaaacaggg
       61 gccgggccat gcgcccagcc cctttcggtt atgcgtcttc ttccgcgttg ccgggggccg
      121 aaacaggctg catctcggcg taagtctccg gcgggaaggt atagcggccg cggtatttct
      181 cgtaccgctg ctcaaatttc acgctgccgc catgcttgac ggaagccagg tactcgtggg
      241 tatcgaactt atcggcggcg acctcgatca tcgcaacagc gatgttggaa cagtggtcag
      301 cgatacgctc gtagttggtc agcagatcat ccagcacaaa gccgtactcg atggtgcata
      361 caccagcctg caggcgggca atatggcggg tcttgacctc gcgcaccaga ccgtccacga
      421 cctgctccag cggctcgatc ttgccggcgg cgtagacatc gcccttctgg aaggcatcga
      481 cggtgcggtt gacgatgtcc tgcacggcgg cttccagcac gcccaggtcg cccagggctt
      541 cctcgctgaa ctggatggat ttatcacgga tctcctcggc cgtcttgatc aggttgacgg
      601 catggtccga gacgcgctcg aaatcgccga tggtgtgcag cagggtgttg atcgtgcggt
      661 tatcttcggt ggaaagatgc ttggcactga gcttgaccag gtaggtgccc agcacatcct
      721 cgtaatggtc gacggcgtcc tctttgcgct cgacctcgtc ggcgatggca tcgttccact
      781 tatgagtggt ggacatggcc tgcagcagcg aggtgcggca gatctcggcc atatcgccgc
      841 cggtgagcat ggcgcgggcg acagccacag acggagttgc cagcagacga tcgtccagca
      901 gggagttctc ctgctttttg tcggcggggg agtcgggcac ggtcatgatg gccagctttt
      961 ccagcaggcg gttgaagggc agcaggatgg cggtggtgac tatgttaaaa acggtatgca
     1021 ccatggcgat gccgaaggta ttggccgtcg cattaaagaa gctgaaacag atcagcgaat
     1081 gcaggccgta gaagccgcac aggaagataa tggtgccgat gacgttgaaa tagagatgga
     1141 cgaaggcagt gcgcttggcg tttttgttcg caccggccga ggagatcagc gctgtaacgg
     1201 tggtgccgat gttctggccc atgatgatgg gcacggcggc ggagtaggtg acagcacccg
     1261 tgctggtcag cgcctgcagg atgcccaccg ccgccgagga cgactgcagc acggcagtca
     1321 gcacagcgcc ggcaatgacg cccagcaccg gattgctgaa gctgaggaag agattcatga
     1381 accagggctc atcggccagg ggagacatgg cgttggacat agtgttcatg ccggccatca
     1441 gaatggcgaa acccagcagg atcttgccta cgccgcgctt gcggtcctta ccgaccatat
     1501 acagccagat gccgatgaag cccaggatcg ggctccaggc attggggttg agcattttgg
     1561 tgataaagct ctcgccctcg aggcccgaca aagacagcag ccagccggta acagtggtac
     1621 caatgttggc acccatgata acgccgatgg cctggtgcag ttccatcaga ccggagttga
     1681 cgaagccgac cgccataacg gtggtagcgc cgctggactg aataacggcg gtgaccgcca
     1741 cgcccagcag cagcgcccgg atggggctgg aagtcatggt ggagaggatg ccctgcaatt
     1801 tgctgccggc ggtctgctcc agcgccttgc ccatcaggtc catgccatac aggaacatag
     1861 cgatgccgcc catcagggtg aacacattaa aaatggtcat cggattacac gtccattctc
     1921 tttttaggca gcgccgccgt tccccgcgcc caaaacgcag gcctatgcgg tgctgctttt
     1981 atttctctat accgttgtat attatagctg gtttcggggg catttcacca gcagagatgc
     2041 gtaaaggttt tgtaaagttt gtgtaaaatg ccgcttctgc gtacattttg tcgtctgcca
     2101 ccttatataa actgtacagc cgctgggata tgacccaaaa ttttaccaat tttacagctt
     2161 ttttgcaaaa cagctgcctg cttttcacaa gctttacggg gattgtacct gactatggtc
     2221 gaaaccgccc cggcggtgca gtacaatagc cttgtacccg ggaaaaccca gtaccggcct
     2281 gtgtgccgca atgcaatgcc tctttgggca aaggagaagt acatctatga aacgcaaatc
     2341 tgttgctgct gttgttacc
//
LOCUS       DBMDSQ010000315         2356 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_9942_length_2356_cov_1.485588, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000315 DBMDSQ010000000
VERSION     DBMDSQ010000315.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2356)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2356
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9942_length_2356_cov_1.485588"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..273)
                     /locus_tag="ACZPAX_09410"
     CDS             complement(<1..273)
                     /locus_tag="ACZPAX_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007491899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HIR1114153.1"
                     /translation="MGFFASLINSMPSAVAQGLIWGIMAIGVYLTYRILDVADLTVDG
                     SLGTGGAVCVVMVLNGAPVGLALLAATAAGMLAGLVTGLFHTACGIP"
     gene            complement(393..1436)
                     /locus_tag="ACZPAX_09415"
     CDS             complement(393..1436)
                     /locus_tag="ACZPAX_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR1114154.1"
                     /translation="MKKAVSLMMAAAMTMGLAACGSSASTDTAASTADAASTTEAAES
                     TADSAAAADGKVYNVGICQLVQHEALDAATQGFKDALVEKLGEGNVKFDEQNASGDSA
                     NCATIVNGFVSNGVDLILANATAPLQAAAQATADIPVLGTSITDYATALDISDWTGTV
                     GNNISGTSDLAPLDQQAAMIQEMFPNAKTVGLLYCSAEPNSVYQCDVIEGYLTEMGLE
                     TTRYAFTDTNDVTSVTQAAAAASDVIYIPTDNTAASNTEAIANVVIPEKVPVVAGEEG
                     ICKGCGVAALSISYYDLGYKTGEMAAEILADGADVSTMPVAFAPNVTKKYNAANCEAL
                     GITPPDGYEAIAD"
     gene            complement(1677..2150)
                     /locus_tag="ACZPAX_09420"
     CDS             complement(1677..2150)
                     /locus_tag="ACZPAX_09420"
                     /inference="COORDINATES: protein motif:HMM:NF012579.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HIR1114155.1"
                     /translation="MKVSELMDPKGVLLYAKIDNSADALGILVELQEGIGVITNGNAY
                     YNAVSQRETFGGTTAIGGGIALPHASNAGVSAPGISALLLRRGVDWGAPDGQPVDLVF
                     MIAVPPKSESLHLQILARLVNLLGRRDLVEALRTASNPQRFVELIAKTENACFAD"
     gene            complement(2175..>2356)
                     /locus_tag="ACZPAX_09425"
     CDS             complement(2175..>2356)
                     /locus_tag="ACZPAX_09425"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019887.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR1114156.1"
                     /translation="VVIGDYYNDLEIMREAGYAVAVANAPAEVKAAADHVTTCTCAEG
                     AVGEYLYSLIGKTDE"
BASE COUNT          479 a          682 c          705 g          490 t
ORIGIN      
        1 cgggatgccg caggccgtgt ggaacaggcc cgtaacaagg cccgccagca tacctgccgc
       61 cgtagccgcc agcagggcca gccccaccgg cgccccgtta agcaccataa caacgcagac
      121 cgcaccgccg gtaccgagag agccgtcgac cgtcaaatcg gccacatcca ggatgcggta
      181 ggttaggtag acgccgatgg ccataatgcc ccagataagg ccctgggcca cagcactggg
      241 cattgagttg atcagggaag caaagaatcc catgaataaa acctccgaac ttttttggaa
      301 tcatgctggg gaaacaatgc accaaaacag cggggaaaga gccgtttcaa ccctctcctc
      361 gctgtttatt cgtgcgtgtg attcaggatt ttttagtcgg caatggcctc atagccgtcc
      421 ggcggggtga tgcccagtgc ttcgcagttg gctgcgttgt actttttggt aacattgggg
      481 gcaaacgcaa caggcatggt ggagacatcg gcgccatcgg ccaggatctc ggcagccatc
      541 tcaccagtct tgtagcccag atcgtagtag ctgatggaca gggcggcaac accgcagccc
      601 ttgcagatgc cttcctcacc ggcaaccaca ggaaccttct ccgggataac cacattggcg
      661 atggcctcag tgttggaagc agcggtgtta tcggtgggga tgtagatcac gtcagaggca
      721 gcagcagcgg cctgggtgac ggaggtgaca tcgttggtat cggtgaaagc gtagcgggtg
      781 gtctccaggc ccatctccgt gagatagcct tcaatgacat cgcactggta gacggagttg
      841 ggctcggcgc tgcagtacag caggccaaca gtcttagcgt tcgggaacat ctcctggatc
      901 atggctgcct gctggtccag cggggccagg tcagaggtgc cggagatgtt gttgccgacg
      961 gtgccggtcc aatcgctgat atccagcgcg gtggcgtagt cggtaatgga ggtgcccagc
     1021 accgggatat cggcagtagc ctgggcggcg gcctgcagcg gcgcggtagc gttggccagg
     1081 atcaggtcaa cgccgttgga gacaaaaccg ttcacaatgg tagcgcagtt ggcggaatcg
     1141 ccggaggcgt tctgctcatc aaattttaca ttgccctcgc ccagcttctc gaccaaagcg
     1201 tccttgaagc cctgggtcgc agcgtccagc gcttcgtgct gaaccagctg gcagatgccg
     1261 acattgtaga ccttgccgtc ggcggcagca gcgctgtctg cggtgctctc ggcggcttcg
     1321 gtggtggaag cggcgtcagc ggtggatgcg gcggtatccg tagaagcgga agatccgcag
     1381 gcggccaggc ccatggtcat ggcggctgcc atcataaggg aaactgcttt tttcatgtct
     1441 attcctccaa atagtaccca ctgcgcaacc ttttgtttca ggtgtacact tgcttcctca
     1501 aacaagtccc tccagcgcag ccggtttagt gctttaaagt atagcacact ttagtggttt
     1561 aaagcaagaa caaatgcgta taataccgcc acgctttttg atgttttttg tggatattgc
     1621 cagttcttgt taaaaacgaa catcccggcc tcctgtaaaa gccgggacat tccctgtcaa
     1681 tctgcaaagc aggcgttttc ggttttggca atcagttcca caaagcgctg ggggttgctg
     1741 gcggtgcgca gggcctccac caggtcacgg cggcccagca ggttcacgag ccgcgccaaa
     1801 atctgcaaat gcaggctctc gcttttgggc ggcacggcaa tcatgaacac caggtccacc
     1861 ggctgtccat cgggcgcacc ccagtccaca ccgcggcgca gaagcagtgc cgagatgccc
     1921 ggcgcggaca cacccgcgtt gctggcgtgg ggcagcgcga tgccgccgcc gatggccgtg
     1981 gtaccgccga aggtctcccg ctggcttacc gcgttatagt aggcgttgcc gttggtgatg
     2041 acgccgatac cctcctgcaa ttccaccaga atgccgagcg cgtcggcgga gttatcgatt
     2101 ttggcgtaga gcagcacgcc cttcggatcc atcagttcac tgactttcat cgtcaaaccc
     2161 tctttgtgat gcggttattc gtctgtctta ccaatcaggc tgtacagata ctcgcctacc
     2221 gcgccctcgg cgcaggtgca ggtcgttaca tggtcggcgg cagctttcac ctcggccggg
     2281 gcgttggcaa cagccaccgc gtatccggcc tcccgcatga tctccaaatc gttgtaatag
     2341 tcgccgatga cgacgg
//
LOCUS       DBMDSQ010000316         2349 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_9989_length_2349_cov_1.734431, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000316 DBMDSQ010000000
VERSION     DBMDSQ010000316.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 2349)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2349)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2349
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9989_length_2349_cov_1.734431"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1047
                     /gene="purF"
                     /locus_tag="ACZPAX_09430"
     CDS             <1..1047
                     /gene="purF"
                     /locus_tag="ACZPAX_09430"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HIR1114157.1"
                     /translation="TSDTEVIAYLLTQNRLLTPNIEMAVSRTMDDIEGAYSLVIMTHT
                     KLIAARDPNGFRPLCIGELPDGNGWAFASESCALDAVGAKFVRDVQPGEIVIADHNGL
                     RSIVDHCGTAPHTMCVFEYIYFARPDSIIEGTCVHEARLQAGRFLAQEHPVEADVVIG
                     APDSGLDAALGYAQESGIPYGVGFIKNKYVGRTFIQGSQAQRESSVRIKLNAISSTVK
                     GKRVVLVDDSIVRGTTSARTIRLLREAGAAEVHYRISAPPFVHPCYFGTDIPDEKDLI
                     ATGHTVEEINKMVGSDTLGYLSIEHVQQLAIHSKCGFCTGCFSGKYPVNPPTETMDIV
                     YDKPLSVSNPNKKL"
     gene            1058..2098
                     /gene="purM"
                     /locus_tag="ACZPAX_09435"
     CDS             1058..2098
                     /gene="purM"
                     /locus_tag="ACZPAX_09435"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HIR1114158.1"
                     /translation="MEKSYSASYAAAGVDITAGYRSVELMKQYVARTMTENCIGGLGG
                     FGGLFELDCTGMEHPVLISGTDGVGTKLRIAMLLDKHDTIGIDCVAMCVNDVICAGAK
                     PLVFLDYIACGKNIPEKIAEIVKGVAEGCVQAGCSLVGGETAEHPGMMPEDEYDLAGF
                     TVGVVDKAKILDNSTMQPGDVIIALPSTGVHSNGFSLVRKIFDIDNNPDVLKKTFDGM
                     DKPLGEALLAPTRIYVKPVLAVMDEITVKGVSHITGGGFYENIPRSLKKGCAARIAKA
                     DVRTPALFDVMQREGSISEHDMFNTFNMGVGMVLTVAPEDADKALEILKVHGEDAYRL
                     GTIVEGDGVVLV"
     gene            2095..>2349
                     /gene="purN"
                     /locus_tag="ACZPAX_09440"
     CDS             2095..>2349
                     /gene="purN"
                     /locus_tag="ACZPAX_09440"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005944461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HIR1114159.1"
                     /translation="MKRVAVLVSGGGTNLQALLESEQRGENPNGKIVLVVASKPGVYA
                     LERAANFGVESAVVSRRDYASSADFDAALLGVLQAHDIDVV"
BASE COUNT          468 a          733 c          692 g          456 t
ORIGIN      
        1 acatcggaca ccgaggtcat tgcctatctg ctgacccaga accggctgct gacccccaac
       61 attgagatgg ctgtcagccg cacgatggac gacattgagg gcgcgtactc cctcgtcatc
      121 atgacccaca ccaagctgat tgcggcgcgt gaccccaacg gtttccgccc gctctgcatc
      181 ggtgaactgc ctgacggcaa cggttgggcc tttgccagcg agagctgcgc actggacgct
      241 gtgggcgcga agttcgtccg cgatgttcag cccggcgaaa tcgtcattgc cgaccacaac
      301 ggcctgcgtt ccatcgtgga tcattgcggc accgcgcccc acacgatgtg cgtgtttgag
      361 tacatctact ttgcccgccc ggattccatc atcgagggta cctgcgtcca cgaggcccgc
      421 ctgcaggctg gccgtttctt ggcacaggag cacccggtcg aggctgatgt tgtcatcggt
      481 gcaccggact ccggcctgga cgccgcgctg ggttacgccc aggagagcgg catcccctac
      541 ggcgttggct tcatcaaaaa caaatacgtc ggccgtacct tcattcaggg cagccaggcc
      601 cagcgcgaga gtagcgtgcg catcaagcta aatgccattt cgtccacggt caaaggcaag
      661 cgggtcgtcc tcgtcgatga ctccatcgtg cgcggcacca ccagcgcccg caccatccgc
      721 ctgctgcgcg aagccggggc cgccgaggta cactaccgca tcagcgcccc gccgtttgtc
      781 cacccctgct acttcggcac cgacattccc gacgagaaag atttgattgc caccggccat
      841 accgtggagg aaatcaacaa gatggtcggt tccgatacgc tgggatacct gagcatcgag
      901 catgtccagc agctggccat ccacagcaag tgcggcttct gcaccggctg ctttagcggc
      961 aaatacccgg tcaacccgcc caccgagacc atggatatcg tctacgacaa gccattgagc
     1021 gtgtcgaacc cgaataagaa attgtgagga tttcaagatg gaaaaaagct attctgccag
     1081 ctatgctgcg gccggtgtag atatcaccgc cggttaccgc tctgtggagc tgatgaaaca
     1141 gtacgtggcc cgcacgatga ccgagaactg tatcggcggt ttgggcggct tcggcggcct
     1201 gtttgagctg gactgcaccg gcatggagca tcctgttctg atttccggca ccgacggcgt
     1261 cggcaccaag ctgcgtatcg ccatgctgct ggacaagcac gacaccatcg gcatcgactg
     1321 cgttgccatg tgcgtcaatg acgtgatctg tgcaggcgcg aagcccctgg ttttccttga
     1381 ctacattgcc tgcggcaaga atatccccga gaagattgcc gagatcgtca agggcgtggc
     1441 cgagggctgc gtgcaggcgg gctgctccct ggtgggcggc gagactgccg agcaccccgg
     1501 catgatgccc gaagatgagt acgacttggc aggcttcact gtcggcgtgg tggacaaggc
     1561 caagattttg gacaactcca cgatgcagcc gggcgacgtc attattgcgc tgccctccac
     1621 cggtgtgcat tctaacggtt tctcgctggt tcgcaagatt ttcgacattg acaacaaccc
     1681 cgatgtgctg aaaaagacct ttgacggcat ggataagccc cttggcgagg ccctgctggc
     1741 ccccacccgc atctatgtca agcccgttct ggccgttatg gacgaaatca cggtcaaggg
     1801 agtttcccac atcaccggtg gcggcttcta cgaaaacatt ccccgcagcc tgaagaaggg
     1861 ctgcgccgcc cgcattgcca aggccgatgt acgcacccct gccctgttcg acgtgatgca
     1921 gcgtgagggc agcatctccg agcacgatat gttcaacacc ttcaacatgg gcgttggcat
     1981 ggtgctgacc gtcgcgcccg aggacgccga caaggccctc gagattttaa aagtccacgg
     2041 cgaggacgcc taccgtctgg gcaccatcgt cgagggtgac ggggtcgtgc tggtatgaag
     2101 cgtgtagcag ttctggtctc cggcggcggc acaaatttac aggccctgct ggaaagcgag
     2161 cagcgcggcg agaaccccaa cggtaaaatc gttctggtcg tcgccagcaa gcccggcgtc
     2221 tatgcactgg agcgcgccgc gaacttcggc gtggaatccg ccgtcgtcag ccgcagggac
     2281 tacgccagca gcgccgattt tgacgccgcc ctgctgggcg ttttacaggc ccatgacatt
     2341 gatgtggtc
//
LOCUS       DBMDSQ010000317         2346 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_10002_length_2346_cov_1.885969, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000317 DBMDSQ010000000
VERSION     DBMDSQ010000317.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 2346)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2346)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2346
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10002_length_2346_cov_1.885969"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..545
                     /locus_tag="ACZPAX_09445"
     CDS             <1..545
                     /locus_tag="ACZPAX_09445"
                     /inference="COORDINATES: protein motif:HMM:NF014657.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HIR1114160.1"
                     /translation="SACVYYLSNLLRCTMQFNLRTFLESGRKPYMAHFEADFSQADFG
                     GYSVNAPAICDFTATLTEDGAALLLCVKADVDAECARCLEPITRDYDFQREYFVRDRD
                     LEDPDFELPCNDNGCLDLEELSYQELIFEVPAVLLCSADCQGLCPICGRKKAAGCSCQ
                     PADNAAPVDARLSILKQLLS"
     gene            570..755
                     /gene="rpmF"
                     /locus_tag="ACZPAX_09450"
     CDS             570..755
                     /gene="rpmF"
                     /locus_tag="ACZPAX_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HIR1114161.1"
                     /translation="MAIVPKRKHSQARRDKRRSNVWKLEAPTLVKCPQCGELKVPHKV
                     CGKCGYYKGQEVIKKEA"
     gene            821..2152
                     /locus_tag="ACZPAX_09455"
     CDS             821..2152
                     /locus_tag="ACZPAX_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HIR1114162.1"
                     /translation="MALYVTAVDENSPAEKFGITVGCALLAIDGHPLNDALDYQFYTT
                     PKSFSLELCKDEQHRTVQVEKGEYEPLGCNFKTYLGDEKHSCANHCMFCFIDQLPPGM
                     REPLYFKDDDERLSFLFGNYITLTNLTEHEIDRIIQMHISPIFVSVHTTNPELRVRML
                     ANKRGGEVLKYLPKLAAGGIELNCQLVLCRGVNDDDELRRTLTDLLALRPQVGSIAAV
                     PAGVTDYRDKLFKLTPYDKESAAATLDILEEFARKCRTEYGRSVVYPSDEWYLTAERP
                     LPPAEFYDEFAQLEDGVGMWRLYHDTFLEELENYSGLVLPHSMDVVTGTLAGPLIQDC
                     ADALMQRYPQVKITVHEIKNEYFGGNVSVAGLITGTDIIKQCGGNLHSDLLGVPEVMI
                     RDEKGQFLDDITLEELGRTLGCRAISIPTDGGGCCKAMLTSHKRKTIKFKR"
     gene            2179..>2346
                     /locus_tag="ACZPAX_09460"
     CDS             2179..>2346
                     /locus_tag="ACZPAX_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015563709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HIR1114163.1"
                     /translation="MAKPIVAIVGRPNVGKSTIFNKLTGQRLAIVEDTPGVTRDRIFC
                     DCEWSGHKFLLV"
BASE COUNT          537 a          644 c          678 g          487 t
ORIGIN      
        1 cttccgcttg tgtatactat ttaagcaacc ttttgaggtg tactatgcaa tttaatcttc
       61 gcacttttct ggaaagcggc cgcaagccct acatggcaca ttttgaggct gacttttccc
      121 aggcggactt tgggggatac agcgtaaacg ccccggcaat atgtgacttt acggcgacgc
      181 tcaccgagga tggcgcagcg ttgctgttgt gtgtaaaggc cgatgtagat gctgagtgtg
      241 cccgttgcct ggaacccatc acccgcgatt acgatttcca gcgcgagtat ttcgtgcggg
      301 atcgtgattt ggaagacccg gattttgaac tgccgtgcaa cgataatggc tgtctcgacc
      361 tcgaagaact ctcctatcag gagttgattt ttgaggtacc cgcggtactg ctgtgcagtg
      421 ctgattgtca aggtctatgc cccatctgcg gacgaaagaa ggcggcaggg tgttcctgcc
      481 aaccggcaga caatgctgcc cctgtggatg caaggcttag catattaaaa caactattaa
      541 gttgaatttc accaaggagg tgtccgatta tggctatcgt acccaagaga aagcattctc
      601 aagcccgccg cgataaacgt cgctctaatg tctggaaact ggaggcgcct acgctggtga
      661 agtgcccgca gtgtggcgaa ctgaaggttc cccataaggt ttgcggcaag tgtggctact
      721 acaaaggcca ggaagtcatc aagaaagagg cgtaagtctc ttgcgtgggg agggcgatgc
      781 cttccccatt ttttgttgtg attacaagga gtgtgaagcg atggccctgt acgtgacggc
      841 ggtggacgaa aattccccgg ctgaaaaatt cggcatcacc gtaggctgcg cactgctggc
      901 catcgacggc caccctttga acgatgcgct ggattaccag ttctatacaa cgccgaaaag
      961 tttctctctg gagctctgta aggacgaaca gcaccgcacc gtgcaggtgg aaaaaggtga
     1021 gtatgagccg ctgggctgca acttcaagac ctacctgggc gacgaaaagc acagctgcgc
     1081 caaccattgt atgttctgct tcatcgacca gctgccgccc ggcatgcgcg aaccgctcta
     1141 ttttaaagat gacgacgagc gcctgagctt ccttttcggc aactatatca cgctgacgaa
     1201 cctgaccgag cacgagatcg accgtatcat ccaaatgcac atcagcccga tttttgtctc
     1261 agtccacacg acaaacccag aactgcgggt gcggatgctg gcgaacaagc gcgggggcga
     1321 ggtgctgaaa tacctgccca agctggcggc gggcggaatc gagctgaact gtcagcttgt
     1381 gctctgccgc ggcgttaacg acgatgacga actgcgccgc accctgacag atctgctggc
     1441 cctgcgcccg caggtgggca gtattgccgc cgtgcccgcc ggcgtgacgg actaccgcga
     1501 taagctgttc aagctgacgc cctacgataa ggaatctgcc gccgctacac tggatatcct
     1561 ggaggagttc gcccggaaat gccgcactga gtacggccgc agcgtcgtct accccagcga
     1621 tgagtggtat ctgaccgcgg agcgcccgct gccgcccgct gagttctacg acgagtttgc
     1681 ccagctggag gacggcgtgg gcatgtggcg gctctaccac gataccttcc tggaagaact
     1741 ggaaaattac tccggcctgg tcttgcccca cagcatggac gttgtcaccg gcacgctggc
     1801 ggggccgctg attcaggatt gcgcagacgc actgatgcag agatacccgc aggtaaagat
     1861 taccgtgcat gagatcaaaa atgagtattt cggcggcaac gtcagcgtgg cggggcttat
     1921 taccggtacg gatatcatca agcagtgcgg cggcaatctg cacagcgacc tgctgggcgt
     1981 gcccgaggtc atgatccgcg acgaaaaagg ccagtttctc gacgatatca cgctggaaga
     2041 actgggcagg acgctgggct gccgggccat cagtatcccc accgacggcg gcgggtgctg
     2101 taaggccatg ctgaccagcc acaagcgcaa aacgataaaa ttcaaacgat aagagagtag
     2161 agagggagga gagcagacat ggcaaaaccg attgtagcca tcgtgggccg ccccaatgtg
     2221 ggcaagtcca ccatcttcaa taaactgacc ggccagcgcc tcgccatcgt cgaggacacc
     2281 cccggcgtca cccgcgaccg cattttctgc gattgcgagt ggtccggcca caaatttttg
     2341 ctggta
//
LOCUS       DBMDSQ010000318         2343 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_10016_length_2343_cov_1.690455, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000318 DBMDSQ010000000
VERSION     DBMDSQ010000318.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 2343)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2343)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2343
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10016_length_2343_cov_1.690455"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(27..449)
                     /gene="mscL"
                     /locus_tag="ACZPAX_09465"
     CDS             complement(27..449)
                     /gene="mscL"
                     /locus_tag="ACZPAX_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009984129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HIR1114164.1"
                     /translation="MKKFFEEFKAFALKGNMMDMAIGVIIGGAFSTLVTSLTDNLISP
                     ILGILGKVNFDAMMWDVFGDGKLVFKYGAFITAVINFLIMAFVLFCIVKAMNKLMEMG
                     KHEEAAPAEPEAPAAPTQEELLADILAELKKQNEEKTA"
     gene            complement(487..1308)
                     /locus_tag="ACZPAX_09470"
     CDS             complement(487..1308)
                     /locus_tag="ACZPAX_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009324941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HIR1114165.1"
                     /translation="MSECTHDCSSCSASCDHRAPQHEPPHERSRIQKVIGVVSGKGGV
                     GKSMTSAMLAVSMRRLGFKAGVLDADITGPSIPRLFGVKGPATGDGTSINPVCSRTGV
                     EIMSINLLLDDPEAPVVWRGPVIAGAVKQFWQEVVWDVDFLFVDMPPGTGDVPLTVFQ
                     TLPVDGIVIVSSPQELVGMIVGKAVQMAQMMHVPILGLVENMSYAVCPDCGKHINVFG
                     DSHVDEIADKYKLPVLAKMPIDPELAKEADAGMIEVFAGDYLDNAAQTVAKLLKK"
     gene            complement(1409..2218)
                     /locus_tag="ACZPAX_09475"
     CDS             complement(1409..2218)
                     /locus_tag="ACZPAX_09475"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010292243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HIR1114166.1"
                     /translation="MNQATTIAAYLREKLPFAPDIALVLGSGLGGLAEQIENAVIIPY
                     RSVPGFPVSTAPGHAGQFVAGQLAGKNVLCMQGRFHYYEGHDMAAIALPVRVFKALGC
                     RALVLTNAAGGVNWDFNVGDIMLITDHINFMGANPLRGTNDDSIGPRFCDMTHTYTPE
                     LQEKALSVAKKQNILLQKGVYLGYMGPSFETPAEIRAFRVLGADAVGMSTVPEAIAAS
                     HCGLPVVALSLITNMAAGMAGKRLDGAEVIEIADQRGPVFQKLVKGIVEVL"
BASE COUNT          487 a          690 c          673 g          493 t
ORIGIN      
        1 cgctgttgtt ggtaataaag cgaaaattac gccgtttttt cctcgttctg ctttttcagc
       61 tcagcaagga tgtcggccag cagctcttcc tgcgtggggg cggcgggggc ctccggctca
      121 gcgggggcgg cttcctcgtg cttgcccatc tccatcagtt tgttcatggc tttgacgatg
      181 cagaacagca caaaagccat gatcaggaag ttgatgacag ccgtgataaa ggcaccatac
      241 ttaaagacca gcttgccatc gccgaaaacg tcccacatca tggcgtcaaa gttcaccttg
      301 cccaaaatgc ccaggatggg gctgatgagg ttgtcggtca gggaagtgac cagcgtggag
      361 aacgcaccgc cgatgataac accgatggcc atatccatca tgttgccctt cagggcgaag
      421 gccttgaact cttcaaagaa ctttttcata agcaggtgcc tcctcaaaat ttaacgccgc
      481 agcgttttac ttcttcagca gctttgccac ggtctgggct gcattgtcga ggtaatcacc
      541 cgcaaagacc tcgatcatgc ccgcgtcggc ctccttggca agctcgggat cgatgggcat
      601 tttcgccagc accggcagct tgtacttgtc ggcaatctcg tccacatggc tgtcgccaaa
      661 cacgttgata tgcttgccgc agtcggggca gacggcgtag ctcatgttct cgaccaagcc
      721 gaggatgggc acgtgcatca tctgggccat ctggacggct ttgccgacga tcatgccgac
      781 cagttcctgc gggctggaca cgatgacgat gccgtccacc ggcagcgtct ggaagacggt
      841 cagcggcaca tcgccggttc ccgggggcat atcgacgaag agaaagtcaa cgtcccacac
      901 aacctcctgc cagaactgct tgacagcgcc cgcaatgacg gggccgcgcc agacaacggg
      961 ggcctcgggg tcatcgagca gaaggttgat gctcataatt tccacgccgg tgcggctgca
     1021 aaccggattg atgctggtgc cgtcgccggt ggcgggaccc ttgacgccga acaggcgggg
     1081 gatggaagga ccggtgatgt cagcgtccag cacaccggcc ttgaagccga ggcggcgcat
     1141 cgagacggcc agcattgcgc tggtcatgct tttgccgacg ccgcccttgc cggaaacgac
     1201 gccgatcact ttttggatac ggctgcgctc gtgcggcggc tcatgctgcg gtgcgcggtg
     1261 gtcacagctg gcactgcagc tgctgcaatc gtgggtacat tcgctcatgg tatgtttgct
     1321 cctttaaaaa ttgctctttc tccagtttac caaaaatcgc gccggaatgc aagaacactc
     1381 acaaatttaa aagcgacctg gcatgatttt ataacacttc cacgattccc ttgaccaact
     1441 tctggaacac cggaccgcgc tggtcggcaa tctcgatgac ttcggccccg tccagccgct
     1501 tgcccgccat cccggcggcc atgttggtaa tcaggctcag tgccaccacc ggcagaccgc
     1561 agtggctggc ggcgatagcc tcgggcacgg tgctcatgcc cacggcgtcc gcacccaaaa
     1621 cgcgaaacgc gcgtatctcg gcgggagtct caaagctcgg ccccatgtag ccgaggtaga
     1681 cgcccttttg cagcaggatg ttttgctttt tggcaacgga aagcgctttt tcctgcaatt
     1741 ccggcgtgta ggtgtgggtc atatcgcaaa agcgcggacc aatgctgtca tcgtttgtgc
     1801 cgcgcagggg gttcgcgccc ataaagttga tgtggtcggt gatgagcatg atgtcgccga
     1861 cattgaaatc ccagttcacg ccgcccgccg cgttggtcag caccagcgca cggcagccca
     1921 gtgccttgaa cacccgtaca ggcagggcaa tggcggccat gtcgtggccc tcataatagt
     1981 ggaaccgccc ctgcatacac aggacgtttt tgcccgccag ctgcccggcc acaaactgcc
     2041 cggcgtgccc cggcgcggtg gacacaggga accccggcac gctgcggtag gggatgatga
     2101 cggcattttc aatttgctct gccagcccgc ccaggcccga ccccagcaca agggcaatat
     2161 ccggggcgaa cggtaatttt tcccgcaagt atgcggcaat ggtagtggct tgattcatgg
     2221 taaaaatctc ccattttatt tgaggcaata ccgcataatt ctaagtgccg atacggcgag
     2281 cgaggtgcgg cagctgctaa gccaaaagcg cagataatac tggatgtctt atcgagcatt
     2341 ttg
//
LOCUS       DBMDSQ010000319         2320 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_10142_length_2320_cov_1.629563, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000319 DBMDSQ010000000
VERSION     DBMDSQ010000319.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 2320)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2320)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2320
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10142_length_2320_cov_1.629563"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1042
                     /locus_tag="ACZPAX_09480"
     CDS             <1..1042
                     /locus_tag="ACZPAX_09480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1114167.1"
                     /translation="LSGVEFSISLLLSRLTLILHDPQYNFTTAQRMALLAGQALTVAR
                     SLLWPLLASAAVCAALYLIKKQPVTVGRLLNLWAAFAMVQCLLRAVKDGSLDERQFVP
                     VVVLAGAWAFWRGRGRPGNAELFWLGYLPGLAAYAMILRSTLLGLAPTFMYLTWPAVC
                     GMLALVNHADGGDNAAKSRRAEGVLCLAAMLAFLLVCRVWCVQTTGWKSADITDTPLV
                     RITTGPAKGIYADAKAADMQECLYEALAPYAGQPILQAIGEQHGLGFLMADGTLTVAQ
                     ASVISGTDSDPRFEQYYTELPDKQPDVILYDDAEVRDMTEFHEWIEANFTITQRYTVQ
                     HGTASLQVLVCG"
     gene            1124..1918
                     /locus_tag="ACZPAX_09485"
     CDS             1124..1918
                     /locus_tag="ACZPAX_09485"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004052254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR1114168.1"
                     /translation="MEKLLVFIPAYNCEKQVTRVLSQLLDKRIAPWVGECIVVNNRAT
                     DGTESAVQDWMARHPDAPVRLLRNDQNYGLGGSHKVAFGYAAAHGYEHLVVLHGDDQG
                     AIADLLPILEDGTYKNYDCCLGSRFMKGSKIGGYSALRVVGNHGFNWLFSAVARKKIT
                     DLGSGLNLYAVEPLKTEYYKKFPDTLYFNDCMILALCQMKQRVLFFPISWREEDQVSN
                     NKLTNFGVSLLKLCGKYLAGPKAFVEREWRDNIIENYSYEVVASNL"
     gene            1933..>2320
                     /locus_tag="ACZPAX_09490"
     CDS             1933..>2320
                     /locus_tag="ACZPAX_09490"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007048315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR1114169.1"
                     /translation="MKVLVTGAAGFIGGQLWHTLWKRGDEVVGIDNFSYGSMDNLKFD
                     DHDFGGEVRKMDIRDKAGMLALFEAEKFDVVYNIAGIAPLPDCQSDPVQAIEVNTLGL
                     VNLLDVTVDEVGILTPSFIILEPDYLI"
BASE COUNT          444 a          661 c          742 g          473 t
ORIGIN      
        1 gctctccggc gtggagttca gcatctcgct gctgctcagc cgcctgacgt tgattttgca
       61 cgacccgcag tacaatttca ccaccgccca gcgcatggcc ctgctggccg gccaggcatt
      121 gaccgtagca cggtcgctgc tctggccgct gctggcctcc gccgccgtct gcgcggcgct
      181 ctaccttatt aaaaagcagc ctgttacggt ggggcggctg ctcaacctgt gggcagcctt
      241 tgccatggtg cagtgcctgc tgcgtgccgt taaggacggc agcctggacg agcgccagtt
      301 tgtgccggtg gtggtgctgg ccggggcgtg ggcgttctgg cgcggccgcg gcaggcccgg
      361 caacgccgag ctgttctggc tgggttatct gccggggctg gcggcctacg ccatgatcct
      421 gcggtcgacg ctgcttggat tggccccgac ttttatgtac ttgacatggc ccgccgtctg
      481 cggtatgctg gcgctggtga accatgcgga tggcggggac aatgccgcaa aatcccgccg
      541 cgccgagggt gtgctttgcc tggccgcgat gctggcgttt ttgctcgtct gtcgcgtctg
      601 gtgcgtgcag acaaccggct ggaaatcggc tgacatcacc gacacgccgc tggtgcgcat
      661 caccaccggc ccggccaagg gcatctatgc cgacgccaaa gccgccgaca tgcaggagtg
      721 tctgtatgaa gcgctggcgc cctacgcggg ccagccgatt ttgcaggcca tcggcgagca
      781 gcacggcctg ggctttttga tggcggacgg cacgctgacc gtggcgcagg cgtcggtcat
      841 ttccggcacc gacagcgacc cgcggtttga acagtattac accgaactgc ccgataaaca
      901 gcccgacgtc attttgtacg atgacgccga ggtgcgggat atgacggagt tccacgagtg
      961 gatcgaagcg aattttacca tcacacagcg ctataccgtg cagcatggca ccgcgagttt
     1021 gcaggtgctg gtctgcgggt gatgggttgt agttgtgccc ggtgtgatgt ataataatgc
     1081 agagcggtat gtccgccccg ataatgatgc aaaggagcag aacatggaaa aactgctggt
     1141 atttatccct gcctacaact gcgaaaagca ggtcacgcgt gtgctttccc agctgttgga
     1201 taagcgcatt gccccctggg tgggggagtg catcgtggtg aacaaccgcg ccaccgacgg
     1261 caccgagagc gccgtgcagg actggatggc ccgccaccct gacgccccgg tgcgcctgct
     1321 gcgcaacgac cagaactacg gcctgggcgg cagccataag gtggcctttg gctatgcggc
     1381 ggcccatggg tatgagcatc tggtggtgct gcacggcgac gaccaggggg ccattgcgga
     1441 cctgctcccg attctggaag atggcacata caaaaactac gactgctgcc tcggctcccg
     1501 ctttatgaag ggcagcaaga tcggcgggta cagcgccctg cgcgtcgtgg gcaaccacgg
     1561 cttcaactgg ctgttcagtg cagtggcccg caagaaaatt accgatctgg gcagcgggct
     1621 gaacctgtac gcagtcgagc cgctgaaaac cgagtactac aaaaagttcc cggatacgct
     1681 gtattttaac gactgcatga tcctggcgct ctgccagatg aaacagcggg tgctgttctt
     1741 ccccatcagc tggcgcgagg aggatcaggt cagcaacaac aagctgacca acttcggcgt
     1801 cagcctgctc aagctctgcg gtaagtatct ggccgggccg aaggcgtttg tagagcgcga
     1861 gtggcgcgac aacatcattg agaattacag ctacgaggtc gtagcgtcca acctgtaagg
     1921 agggtcataa gtatgaaagt tctggtaacg ggtgcagccg gttttatcgg cggtcagctg
     1981 tggcatacgc tgtggaagcg cggggacgag gtcgtcggca tcgacaactt ctcctacggc
     2041 agcatggaca atctgaagtt cgacgaccat gatttcggcg gtgaagtgcg gaaaatggac
     2101 atccgcgaca aggccgggat gttggccttg tttgaggcgg agaaattcga tgttgtgtac
     2161 aatattgcgg gcattgcgcc gctgccagac tgccagagcg acccggtgca ggccatcgag
     2221 gtcaacaccc tgggcctggt caatctgctg gatgttacgg tggacgaagt cggcatactc
     2281 accccttcct ttattatact tgagcccgat tacttgatag
//
LOCUS       DBMDSQ010000320         2306 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_10220_length_2306_cov_1.602268, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000320 DBMDSQ010000000
VERSION     DBMDSQ010000320.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 2306)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2306)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2306
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10220_length_2306_cov_1.602268"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(127..1449)
                     /locus_tag="ACZPAX_09495"
     CDS             complement(127..1449)
                     /locus_tag="ACZPAX_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HIR1114170.1"
                     /translation="MWYDEAVVYQIYPLGLCGAPLQNDGDQSQADNHRILRVLDWVEH
                     IKRLGATCVLFNPLFESDAHGYDTRDYNKVDCRLGTNDDLKQVCAALHAAGIKILFDG
                     VFNHVGRGFWAFRDVQEKRWDSPYKDWFHISFDGNTNYNDGFWYEAWEGCNELVKLNL
                     QNPAVKNYLFDVVRGWVRDYDIDGLRLDVAYCLDLGFLAELRGLANDIKPEFALVGET
                     LHGDYNRWMNDHACHSVTNYECYKGLYSSFNTGNMHEISYSLNRQFGAEQWCLYTGKH
                     LLSFVDNHDVTRIATILTNKNCLKPIYGLLFGMPGVPSVYYGSEWGMEGDKRNGDPTL
                     RPAVEKPTENDLTAWVAALAHARTGSKALCRGSYRNVLVQPKQLIFERYADGERVLVA
                     INADGNPFDAHFDAGCGRAVDLITGVDHDFGGGSTLEPYSCHFWKCER"
     gene            complement(1468..2007)
                     /locus_tag="ACZPAX_09500"
     CDS             complement(1468..2007)
                     /locus_tag="ACZPAX_09500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1114171.1"
                     /translation="MRKTESQKIALCGMLGALSVVLLLIGSALQIGTYAAPMLAAFLI
                     IPVLEEYGPRYALTLYATVSILAVLFVPETELAFFYVLVMGYYPVLRVKLNTLKNTVL
                     RWVIKFAVFNAATVLVYLLLFALLGPAVLDELLADGTAMLVALLAAGNLSFWLCDRAL
                     AALTRYYHLVVKPKLKKKF"
     gene            2069..>2306
                     /locus_tag="ACZPAX_09505"
     CDS             2069..>2306
                     /locus_tag="ACZPAX_09505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1114172.1"
                     /translation="MLDILHNPAWVRWILSVVFGKVLGTTPFYGDKTGLPLFPATYNP
                     LCRTLWAVSLQGFHLCNCTKGLPVIWTLATLGCST"
BASE COUNT          486 a          714 c          590 g          516 t
ORIGIN      
        1 attatctgcg cttttggctt agcaactgcc gcacctcgct cgccgtatcg gcacttagaa
       61 ttatgcggta ttgccctagt acaaaaatgc acctgcccac acggacaggt gcatttttat
      121 atcgtattat cgttcacatt tccagaaatg acaactgtat ggctctaatg tgctgccgcc
      181 gccaaaatcg tgatcgaccc cggtgatgag gtccaccgcg cggccgcagc ctgcgtcaaa
      241 gtgggcgtca aacggattgc cgtccgcatt gatggctacc agcacccgct caccgtcggc
      301 atagcgttca aaaatcagct gcttcggctg caccagtaca ttgcggtagc tgccccggca
      361 cagcgctttg ctgcctgtac gggcatgggc cagcgcggcc acccaagccg tcaggtcgtt
      421 ctcggtgggc ttctccaccg ccgggcgcag cgtcgggtcg ccgttgcgct tgtcgccctc
      481 catgccccac tcactgccgt agtagaccga cggcacaccg ggcataccga acagcaaacc
      541 gtagatgggt ttcaggcagt ttttattggt cagaatcgtc gcaatacgcg ttacatcgtg
      601 gttatccaca aaactcaaca gatgcttgcc ggtatacagg caccactgct ccgcgccaaa
      661 ctggcggttc aggctataac tgatctcgtg catattgccg gtgttgaagc tggaatacag
      721 ccccttgtag cactcgtagt tcgtcacgct gtggcaggcg tggtcgttca tccagcggtt
      781 gtagtcgccg tgcagcgtct cgcccaccag cgcgaactcc ggcttgatgt cgtttgccag
      841 cccgcgcagc tccgccaaaa agcccagatc caggcagtac gcgacgtcca ggcgcaggcc
      901 gtcgatatcg tagtcccgca cccagccgcg cacgacgtca aacaggtagt ttttcaccgc
      961 tgggttctgc aagttcagct tgaccagttc gttgcagccc tcccaggcct cgtaccagaa
     1021 accgtcgttg taattggtgt tgccgtcaaa actgatgtga aaccagtctt tgtaagggct
     1081 gtcccaacgc ttttcctgca cgtcgcggaa cgcccagaag ccgcgcccca catggttgaa
     1141 cacgccgtcg aacaaaattt tgatgcccgc cgcatgcagc gccgcgcaga cctgcttgag
     1201 gtcgtcgttc gtgcccaggc ggcagtcgac cttgttatag tcgcgggtgt cgtagccgtg
     1261 ggcatcgctc tcaaacagcg ggttgaacag cacgcaggtc gcgcccaggc gcttgatgtg
     1321 ctccacccag tccagcacgc gcagaatgcg gtggttgtcc gcctgggact ggtcgccgtc
     1381 gttttgcagt ggtgcgccgc acagccccag cggataaatc tggtaaacta ccgcttcatc
     1441 ataccacatt ttatgttcct ccttgcatca gaacttcttc ttcagttttg gcttgaccac
     1501 cagatgataa tatcgcgtca gcgccgccag cgcccggtcg cacagccaga atgacagatt
     1561 tcccgcggcc aacagcgcca ccagcatggc cgttccgtcg gccagcagct cgtccaatac
     1621 agcaggcccc agcagcgcga acaacagcaa atacaccagc accgtggccg cattgaacac
     1681 cgcaaatttt atgacccaac gcagtaccgt gttttttagt gtgttgagct tcacgcgcag
     1741 caccgggtaa taccccatga ccagcacata gaaaaacgcc agctcggttt ccggcacaaa
     1801 cagcacggcc aaaatcgata cggtcgcgta gagggtcaac gcatagcgcg gtccgtactc
     1861 ctccagcact gggatgataa ggaacgccgc cagcatcggc gcagcgtagg tgccaatctg
     1921 cagcgcactg ccgatgagca gaagcaccac cgacagcgcc cccagcatcc cgcacaacgc
     1981 aattttctgg ctttccgtct tgcgcacagc cccacctcct tgccgattct tgtacattat
     2041 accctatttt gtttcacatt gcaagaaatt gctggatatt ttgcacaatc cggcgtgggt
     2101 gcgttggatt ttatcagttg tttttggcaa ggtactgggt actacaccat tttatggtga
     2161 taagaccgga ttaccacttt tcccagcaac atacaatccg ctgtgccgca cactttgggc
     2221 ggtttcactt caaggcttcc atctgtgcaa ctgtacaaaa ggtttgccgg ttatctggac
     2281 tttggcaacc ttgggttgct caacat
//
LOCUS       DBMDSQ010000321         2277 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_10385_length_2277_cov_1.109835, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000321 DBMDSQ010000000
VERSION     DBMDSQ010000321.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 2277)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2277)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2277
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10385_length_2277_cov_1.109835"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..320)
                     /locus_tag="ACZPAX_09510"
     CDS             complement(<1..320)
                     /locus_tag="ACZPAX_09510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1114173.1"
                     /translation="MRYIFAKDAKDLTQYPVPEKWNVGYVGKILLLFDDVNGDNKTSK
                     EDITDEVLQRYYSNILDNSTPDAPTGLKIERSDATGSGNESIYGRYNVTWNAPTTGSP
                     AIYY"
     gene            complement(550..>2277)
                     /locus_tag="ACZPAX_09515"
     CDS             complement(550..>2277)
                     /locus_tag="ACZPAX_09515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1114174.1"
                     /translation="NTDGTAAATGKVKDCKVGYIELKVQGASNVGASQDAATKMNSAA
                     HIGGIVGRNRGEITGSTVGTKDKTGSIITARSGFVGGVAGSNSGSISTSGGLDTQPLV
                     KQINAWLDVPYKDETTTDENGNEVTAQVVDTDKQNANLNQMVRVLTGKASGEAETKLQ
                     IDFKAMQGFDTLTYGDKNYNTVYTNRRDNQLLVSLRGSTSHGNGLADGYLGGITGFND
                     VTGQITNSASGQWFVYADNINQSWGAVGGVIGQNESNADSTSGLVNFAAVRRFVRGTR
                     GTADDNSNANNDRYATTKGDNAADNYVGGVIGTQENRTGDRWTLEKCINVGTVFNSRS
                     NNAGGVLAYWLSYGGTLTNCYNFGEITTNSNNGESSGTVGGVAGYFNNPVAGTAANLY
                     RCSNYGSVKKMTNGANDVGGVFGKVQLANDHNSDAMTINVIECVNGSNVELQAQSMSV
                     GIFCYIGPWAAIPNVEVNIDRCRNYCTEMWGPVRSAGIWGNRGKGSTSNKKTTITNCF
                     ALYKSKNNTGSIWYPIAYRHTANEQLTGSDNYYMQLDKSFDGYSGINGLQEGLGRTKA
                     TGGMEPGYQ"
BASE COUNT          404 a          628 c          661 g          584 t
ORIGIN      
        1 tggtagtaga tggccgggct gccggtggtg ggggcattcc aggtcacgtt ataacggccg
       61 taaatactct cgttaccgga accggtagca tcactgcgtt cgattttcag tcctgtgggg
      121 gcatccgggg tgctgttgtc caggatattg ctgtagtagc gttggagcac ctcatcggta
      181 atatcttcct tgctggtctt gttgtcaccg ttgacgtcgt cgaagaggag caggatcttg
      241 ccgacatagc cgacattcca tttctccggc acaggatact gggtcagatc cttggcatct
      301 tttgcaaaga tataacgcac agacgaagct tccgagaagc tttggcccgg caaggtggta
      361 atatacgagc tgctcttttt tacagcctgc atcgagacga ccggtctgcc atcgcttgaa
      421 atctttggca gcattgcaaa gaagtgatag aagttgtcgc catcctgggc ggcgttggca
      481 ccgctgtcca caccggcata caggcgatgc gcgtttacgg cggtagtcgc accgccgcct
      541 tcttcgcttt cattggtacc ctggctccat cccccctgta gctttggtgc ggcccaggcc
      601 ctcttgcagc ccattaatgc cggaataacc atcgaagctc ttatccagct gcatgtagta
      661 gttgtcggaa ccggtcagtt gttcattggc tgtatggcga taggcaatcg ggtaccagat
      721 gctgcccgta ttgttcttgc ttttatacaa ggcaaaacaa ttggtgatgg tcgttttctt
      781 gttagaggtg ctgcctttgc cgcggttgcc ccagatgcca gctgaacgga cggggcccca
      841 catttcggtg caatagttac ggcagcggtc aatgttgacc tcaacgttgg ggatagccgc
      901 ccacggacca atatagcaga agatgccgac agacatcgac tgtgcctgta gttcgacatt
      961 ggagccgttg acgcactcga tcacgttgat ggtcatggca tcgctgttat ggtcattcgc
     1021 cagctgtacc ttaccaaaaa cgccgcctac gtcgtttgca ccattggtca tcttttttac
     1081 gctgccatag ttgctgcagc gatacaggtt ggccgccgta ccggcaacag gattattgaa
     1141 gtaacctgcc acgccgccaa cggtgccgga gctttcgccg ttgttggagt tggtggtgat
     1201 ctcgccaaag ttatagcagt tggtcagcgt accgccgtag ctgagccagt aggccagcac
     1261 accacccgcg ttgttggagc ggctgttgaa caccgtgcca acattgatac atttttccag
     1321 cgtccagcgg tcgccggtgc ggttttcctg cgtaccaata acgccgccta catagttatc
     1381 tgctgcattg tcacctttgg tggtagcata gcggtcattg ttagcgttgc tattgtcatc
     1441 cgccgttccc cgcgtgccgc gcacaaagcg gcgcacggcg gcaaagttga ccaggccgct
     1501 ggtggaatcc gcattggatt cgttctggcc gatgacgccg cccacggcgc cccagctctg
     1561 gttgatgtta tcggcgtaga cgaaccactg gccgctggca ctgttggtga tctggccggt
     1621 gacatcgtta aagccggtga tgccgcccag gtaaccgtcg gccaggccat tgccatggct
     1681 ggtgctgccc cgcagcgaga ccagcagctg attgtctctg cggttggtat aaactgtatt
     1741 atagttttta tcgccatagg tcagggtatc aaagccctgc atggccttaa agtcgatctg
     1801 cagttttgtc tctgcctcac cgctggcctt gccggtcagt acgcggacca tctggttcag
     1861 gttggcgttc tgtttgtcgg tgtctaccac ttgggcagtg acttcgttgc cgttttcgtc
     1921 ggtggtggtc tcgtctttat agggcacatc cagccaggcg ttgatctgtt ttacaagggg
     1981 ctgtgtgtcc aggccgccgc tggtggagat gctgccgctg ttggagccgg ccacgccgcc
     2041 cacaaagccg ctgcgggcgg tgatgatact acccgttttg tctttggtgc cgacggtgct
     2101 gccggtgatc tcgccgcggt tgcggcccac gataccgccg atatgggcgg cgctgttcat
     2161 cttggtggca gcgtcctggc tggcgcccac gttggaggca ccctgcactt tcagctcgat
     2221 atagccgacc ttgcagtcct tcaccttgcc ggtggcggcg gcagtgccgt cggtgtt
//
LOCUS       DBMDSQ010000322         2273 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_10397_length_2273_cov_1.734807, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000322 DBMDSQ010000000
VERSION     DBMDSQ010000322.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 2273)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2273)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2273
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10397_length_2273_cov_1.734807"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..897)
                     /locus_tag="ACZPAX_09520"
     CDS             complement(<1..897)
                     /locus_tag="ACZPAX_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009324720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HIR1114175.1"
                     /translation="MKRNGVRRWRSFCGRQKMPPRKGWAAVYCRLSKEDEDKAAAESE
                     SIRNQRALLLDWAATHGYHVYKVYTDEDYSGIDRARPGFNAMLNDARQGKFEVILAKT
                     QSRFTRDMELVERYLHGLFPEWGVRFIAVLDHVDTCDPAGKKARQINGLINEWYLEDL
                     SGNVRAVLDHKRHSGCYIASFALYGYRKDPADHNRLVIDPPAAAVVRRIFALYVQGAG
                     AGKIAQTLNAEAIPPPSVYRAAQSGQPAAKPTLWSKATVRRILSNSTYVGDMEQGRVR
                     KLNYKSKKAIRLPRDEWIIVPGT"
     gene            complement(1023..1985)
                     /locus_tag="ACZPAX_09525"
     CDS             complement(1023..1985)
                     /locus_tag="ACZPAX_09525"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR1114176.1"
                     /translation="MAAVTVVAAVLAAVLLAVSVYLVNYAIGRSGSGGDRDVALEVEA
                     SPDSVEAVIAANKAQCKGKIDAFTQENPGENLAITSQDGLTLHGVCYTHADADSHRWA
                     IVLHGYRGDYTGALCLAEPYYEAGYQVIAPDLRACGESEGSYVGMGWLDREDILQWID
                     YITVRDPQAEIVIHGISMGAATTMMTAGETTPDAVKAYVEDCGYTSVWDVFSSELQLR
                     FGLPEFPILYTASAVAKLQAGYSFTEASALQQVAHCEKPMLFIHGTADDFIPYEMMDA
                     LYNAKPGDNKAELTAEGAGHGEALYALGGTYWDTVFAFLEPYMN"
BASE COUNT          447 a          719 c          596 g          511 t
ORIGIN      
        1 agtgcccggt acgatgatcc actcgtcgcg gggcagccgg atggcctttt ttgatttgta
       61 gttcagcttg cgcacgcgcc cttgctccat gtcgccgaca taggtgctgt tggacaggat
      121 gcgccgcaca gttgccttgc tccacagtgt tggctttgcg gcgggttggc cgctttgcgc
      181 tgcgcggtaa acggacggcg gggggatggc ttcggcgttc aatgtttgcg cgatctttcc
      241 cgcgccagcc ccctgcacat acagggcaaa gatacgccgc accaccgccg ctgcgggcgg
      301 gtcgataacc agccggttgt ggtcggcggg gtccttgcgg tagccgtaca gcgcaaagct
      361 ggcgatgtag cacccgctgt gccgcttgtg gtccagcaca gcccgcacat tgccggataa
      421 gtcttcaaga taccactcgt tgataaggcc gttgatctgc cgcgcctttt tcccggccgg
      481 gtcgcaggta tccacatgat ccagcacggc gataaatcgc acgccccact cggggaacag
      541 gccgtgcaga taacgctcca ctagctccat atcgcgggta aagcgggact gcgttttggc
      601 taggatgacc tcaaatttcc cctgccgcgc gtcgttcagc atggcgttga accccggccg
      661 cgcccggtcg atgcctgagt agtcttcatc ggtgtagact ttgtaaacat ggtagccgtg
      721 cgtcgccgcc caatccagca gcagtgcccg ctgattgcgg atactctcgg actccgccgc
      781 ggctttgtcc tcgtcctcct tgctcaggcg gcagtatacc gccgcccacc ccttacgcgg
      841 gggcattttc tgcctgccgc agaagctgcg ccagcgccgc acgccgttcc gtttcatttt
      901 ggtgcaagaa tttttctatg acgatatatt ccatctgtac tccctccctc tgctgcattg
      961 tacgccgggg cgcggcacgg taacacaaaa acggcgcagc ccgcaggccg cgccgttttg
     1021 catcagttca tatagggttc aaggaacgca aacacggtat cccagtacgt gccacccagc
     1081 gcgtaaaggg cctcaccgtg ccccgcgccc tctgcggtca actccgcttt gttgtccccg
     1141 ggcttcgcgt tgtaaagcgc atccatcatc tcatagggga taaaatcatc tgccgtacca
     1201 tgaatgaaaa gcattggctt ctcgcagtgc gccacctgct gaagcgcact ggcctcggta
     1261 aagctgtacc ccgcctgcag ctttgccaca gcgctggccg tgtacaggat cggaaattcc
     1321 ggcaacccga accgcagctg cagctcgctg gaaaaaacat cccagacgct tgtgtacccg
     1381 caatcctcga catacgcctt cacggcgtcg ggcgtggttt cacccgcagt catcatcgtg
     1441 gtggctgcgc ccattgaaat gccatgaatg acgatttctg cctgcgggtc gcgcaccgtg
     1501 atatagtcga tccactgcaa gatatcctcg cggtcgagcc agcccatgcc cacatagctg
     1561 ccttcgcttt caccgcaggc gcgcagatcc ggtgcaatga cctggtagcc agcctcatag
     1621 taaggttctg ccaggcacag cgccccggtg tagtctccgc ggtagccgtg cagcacaatg
     1681 gcccagcggt ggctgtctgc gtccgcatgg gtgtagcaga caccgtgcag cgttagtcca
     1741 tcttgggaag tgatcgccag gttttccccg ggattttcct gtgtgaaagc gtcaattttt
     1801 cccttacact gcgctttatt cgctgcaatg actgcctcca cgctgtccgg cgaggcttcc
     1861 acttccagcg ccacatcacg gtcgccgcca ctgccgctgc ggccaatggc ataattcacc
     1921 agatacacgc tgaccgccag cagcaccgcc gccaatacgg cagcaacgac tgtaaccgcc
     1981 gccataactt tctttttaaa atttgttttt actttcaatc tgcccatacc tttcaataat
     2041 tactcaacaa taggtgttag tatactgcat tattattgat ttgtcaacaa tgcaggcaca
     2101 aaatatagtc catccggcca cttcgccgtt attgcaattt aaaaccgaag gccgggcaga
     2161 cccggcccct acaacgctat aataaacaaa aacgccctgc tcatcactag gatgaacagg
     2221 gcgtttttac gagaagccgg catctaccta ttttcacagg ccgtttccag cca
//
LOCUS       DBMDSQ010000323         2271 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_10410_length_2271_cov_1.817051, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000323 DBMDSQ010000000
VERSION     DBMDSQ010000323.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 2271)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2271)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2271
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10410_length_2271_cov_1.817051"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            94..1359
                     /locus_tag="ACZPAX_09530"
     CDS             94..1359
                     /locus_tag="ACZPAX_09530"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR1114177.1"
                     /translation="MHILWLVKTTLPQAAVACGLAGASDVSGSWLTGQLAALRTHADL
                     HLTVLCVGKTDASSAADGVDYRIVADAAAFAPLLQAGAFDLVHIWGTEYDAAAMMADA
                     ARAQNLPVLFSIQGVMRDCAAHLCDGVPDAYRHSGAVWHAIDRAVPGELLDNMQANFD
                     ALAAKEAALLHDARHVTGRTGFDRAACAALAPSARYYPCNETLRPLFYTGTLWHAREF
                     AAAPMLFLPQGNYPLKNLHTVIKALPAVLAQYGNAQLQIAGWPPLDKGPLLRPVIDRM
                     FPYKLYCKRLAAQLGVAEHIHYTGPLDASAMRQAYLEADVFLLPSSCENSPNSLGEAM
                     LLGLPCVAAAVGGIPSMLEDDREGYLYGDALDEKALSNAILRVLQSPDGGTAIGQAAR
                     ARALQTHDAARNADDLIHIYETILREEHK"
     gene            1356..>2271
                     /locus_tag="ACZPAX_09535"
     CDS             1356..>2271
                     /locus_tag="ACZPAX_09535"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR1114178.1"
                     /translation="MSIRNVSIIIPCYKAAATLRRAVASALTDAPSDIEILLVDDGSP
                     DDTGALCDELAAADPRVKALHRENGGAGAARNTGLDAASGEWVLFLDADDALLPGLWS
                     ALDGVITDADMILFGLTRVSTGDIQPTDYLPAGEYADLASLGGALSPLLFDTGLLAAP
                     YPKLFRRRTLGGLRFDDRLQINEDVLFNIQFLQISSAIYCLHGVYYKQYDMESGSLSR
                     RLRGDLLDAERITRPALAALLRKSGLDPAPYEHTSRLRACLNQYGLLTGCRGDLPYAR
                     RRALFAEILADNDARAALQERLRNDPNKI"
BASE COUNT          400 a          752 c          700 g          419 t
ORIGIN      
        1 gttatggaat aggctcattg ctggtcacta cggtccgcca tcgttatatc ccttatctgg
       61 tggcaatcat tttggttata gaaggagcac cccatgcaca ttctctggct tgtaaaaacg
      121 acgctgccgc aggcggcggt ggcgtgcgga ctggcagggg ccagcgacgt gagcggcagc
      181 tggctgaccg gccagctggc cgcactgcgc acccacgccg atctgcacct gacggtgctc
      241 tgcgtcggca agaccgatgc cagcagcgca gcggacggcg tggactaccg catcgtggcc
      301 gatgccgccg cctttgcgcc gcttttgcag gccggggcgt ttgaccttgt gcacatctgg
      361 ggcaccgagt acgatgccgc cgccatgatg gccgacgccg cccgggcgca aaacctgccg
      421 gtgctgttca gcatccaggg tgtcatgcgg gactgcgccg cccacctgtg cgacggcgtg
      481 ccagacgcct accgccattc gggcgcggtg tggcacgcca ttgaccgcgc cgtgcccggg
      541 gagctgttgg acaatatgca ggcaaatttt gacgcactgg cggcaaaaga agcggctctg
      601 ctgcacgatg cccgccatgt gaccggccgc acaggtttcg accgtgccgc ctgcgccgcc
      661 ctggccccga gcgcccgcta ctacccctgc aacgaaacac tgcgcccgct gttttacacc
      721 ggcacgctct ggcacgcgcg ggagtttgcc gccgcgccaa tgctgtttct gccccagggc
      781 aactacccac tgaaaaattt gcacaccgtc atcaaggcac tgcccgccgt gctggcccaa
      841 tacggcaatg cccagctgca aattgcgggc tggcccccgc tggacaaagg cccgctgctg
      901 cgcccggtca tcgaccggat gtttccttac aaactctact gcaagcggct ggccgcgcag
      961 ctgggcgtgg cggaacacat ccactatacc ggcccgctgg acgcctccgc catgcgccag
     1021 gcgtatctgg aggccgacgt tttcctgctg ccgtccagct gcgagaacag ccccaacagc
     1081 ctgggcgaag ccatgctgct gggcctgccc tgcgtggccg ccgccgtggg ggggattccc
     1141 tcgatgctgg aagatgaccg cgagggctat ctgtacggcg acgcgctgga tgaaaaagcg
     1201 ctgtccaacg cgatcctgcg tgtgctgcaa agcccagacg gcggcacggc cataggccag
     1261 gccgcccgcg cccgggcgct gcagacccac gacgccgccc gcaacgccga tgatttgata
     1321 catatctacg agactatttt gcgcgaggaa cacaagtgag catccgtaac gtttccatca
     1381 tcatcccctg ttataaggct gcggccacgc tgcgccgcgc cgtagcaagt gccttgacag
     1441 acgctccctc cgacatagag atcctgctgg tggacgacgg cagccccgat gacaccgggg
     1501 cgctttgtga cgagctggcc gccgcagacc cacgcgtaaa agccctgcac cgcgaaaacg
     1561 gcggcgcggg cgcagcccgc aataccgggc tggacgcggc aagcggcgaa tgggtactgt
     1621 ttttggatgc cgacgacgcg ctgctgcccg ggctgtggtc tgcgttggac ggcgtaatca
     1681 ccgacgccga tatgattttg ttcggcctga cgcgggtttc cacaggtgat atccagccca
     1741 cggactacct gcctgcgggc gaatacgccg acctggcctc actgggcggg gcgctctctc
     1801 ccctgctgtt tgacaccggg ctgctggctg cgccctatcc taagttgttc cgccgccgca
     1861 cgctgggcgg cctgcggttt gacgatcggc tgcaaatcaa tgaggatgtt ttatttaaca
     1921 tacaattttt acaaatttcc tctgccattt attgcctgca tggtgtatac tataagcagt
     1981 atgatatgga gtctggcagt ctgtcccgcc gtctgcgggg ggatttgctg gacgccgagc
     2041 gcatcacccg cccggcgctg gccgccctgc tgcggaaaag cggcctggac cccgcgccct
     2101 acgagcacac cagccgtctg cgggcctgcc tgaaccagta cggtttgctg actggctgca
     2161 ggggggatct tccgtatgca aggcgtcgcg cactgtttgc tgagatcctg gccgacaatg
     2221 acgcccgcgc cgccctgcaa gagcggctgc ggaatgaccc aaacaagatt c
//
LOCUS       DBMDSQ010000324         2261 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_10481_length_2261_cov_1.862963, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000324 DBMDSQ010000000
VERSION     DBMDSQ010000324.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 2261)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2261)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2261
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10481_length_2261_cov_1.862963"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..591)
                     /locus_tag="ACZPAX_09540"
     CDS             complement(<1..591)
                     /locus_tag="ACZPAX_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR1114179.1"
                     /translation="MAKKMPEAMDYTPVTYDPQYILMVNHLKKYFPIKGGLLSQTVGH
                     VKAVDGVTFNLRRGCTMGLVGESGCGKSTTGRTILRLSGDKTGGQVLFNGKEVYDMNS
                     AELRELRTKMQIIFQDPFSSLSPRLPVGEIIGEAVREHNLVPKNEFDDYIDQVMDDCG
                     LQPYHKTRYPHEFSGGQRQRICIARALALNPAFVVCD"
     gene            complement(595..1614)
                     /locus_tag="ACZPAX_09545"
     CDS             complement(595..1614)
                     /locus_tag="ACZPAX_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1114180.1"
                     /translation="MKDPANAVEFDNLHTYFFTDTGVVKSVDGVSFEVPIGKTVGVVG
                     ESGCGKSVTSLSLMQLLQRPQGQIVEGEIRLNLGNDKAYDIAKTPTDKMQGLRGNYIS
                     MIFQEPMTSLNPVFRIGAQIEEVIALHEGEGKTKEDIKKRTIEMLEMVGIANSEGVYA
                     MYPHELSGGMRQRVMIAMALACNPKIIIADEPTTALDVTIQAQILDLLRNLKDRINSS
                     IMLITHDLGVIAEMADYVVVMYAGRVVEKGTVEEIFAHPSHPYTIGLMASKPVVGKKV
                     DRLYSIPGKVPNPVNMPDYCYFKDRCEMQLPCCSGEYPCEISLSPTHKVSCYRYYEEN
                     RKAGE"
     gene            complement(1769..>2261)
                     /locus_tag="ACZPAX_09550"
     CDS             complement(1769..>2261)
                     /locus_tag="ACZPAX_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1114181.1"
                     /translation="MPVIIIIGAAMDAMRVDSWKRMLYLMLILGFLGWPSIARLVRGQ
                     ILSLREQEFMLATEACGIKVWHRIFRHLIPNVIPQLIVTCTMGLGSTILTEATLSFLG
                     LGVKYPFASWGNIINDVNNAYVMTNYLFIWIPAGICLLLTVLGFNFVGDGLRDAFDPK
                     MKR"
BASE COUNT          430 a          629 c          714 g          488 t
ORIGIN      
        1 gtcgcagaca acgaacgccg ggttcagggc caaggcgcgg gcgatgcaga tacgctggcg
       61 ctggccgccg gagaactcat gcggatagcg ggtcttgtgg tagggctgca gaccgcagtc
      121 gtccatgacc tggtcgatat aatcatcaaa ctcattcttc ggcaccaggt tatgctcgcg
      181 cacagcctcg ccgatgatct cgccgacggg cagacggggg gacaggctgg agaacggatc
      241 ctggaagatg atctgcatct tggtgcgcag ttcccgcagc tcggcgctgt tcatatcgta
      301 gacttctttg ccgttgaaca gcacctggcc accggtctta tcgccgctca ggcgcaggat
      361 ggtgcggccg gtggtggact tgccgcagcc ggactcgccc accaggccca tggtgcagcc
      421 gcggcgcagg ttaaaggtaa cgccgtccac tgccttgaca tggcccactg tctgggacag
      481 caggccgccc ttgatgggga aatatttttt gaggtggttg accatcagga tgtactgagg
      541 gtcataggtg acgggggtgt aatccattgc ttcgggcatt ttctttgcca tacgtcactc
      601 acccgccttt ctgttttctt cgtagtagcg gtagcagctg accttatggg tgggggacag
      661 gctgatctcg caggggtact cgccgctgca gcagggcagc tgcatttcgc agcggtcctt
      721 gaagtagcag taatccggca tattgaccgg gttaggcacc ttgccgggga tggagtacag
      781 gcggtccact tttttgccga cgaccggctt ggacgccatc aggccaatgg tgtaggggtg
      841 gctcgggtgg gcaaaaattt cctccaccgt gcccttctcg accacgcggc ccgcatacat
      901 gacaacgaca tagtcggcca tttctgcgat aacgcccagg tcgtgggtga tcagcataat
      961 ggacgagttg atgcgatcct tcaggttgcg cagcaggtcc aggatctggg cttggatcgt
     1021 cacgtccagc gcggtggtcg gctcgtcggc aatgatgatt ttcgggttgc aggccagcgc
     1081 catggcgatc atgacgcgct ggcgcatacc gccggacagc tcgtgggggt acatcgcgta
     1141 aacaccctca ctgttggcaa tgccgaccat ctccagcatt tcaatggtgc gcttcttgat
     1201 gtcctctttt gtcttgccct cgccctcgtg cagggcaatg acctcttcga tctgtgcgcc
     1261 aatgcggaac accgggttca ggctcgtcat aggctcctgg aagatcatcg agatatagtt
     1321 gccgcgcagg ccctgcatct tgtcggtcgg ggtcttggca atgtcgtagg ccttgtcatt
     1381 gccgaggttc agacgaatct cgccttcgac gatctgcccc tgggggcgct gcagcagctg
     1441 catcagggaa aggctggtga cagacttgcc gcagccggac tcaccgacga cgccgacggt
     1501 cttgccgatg ggcacttcaa acgaaacgcc gtcgacgctc ttgacgacgc cggtgtcggt
     1561 gaagaaatag gtgtgcaggt tatcgaactc caccgcgttg gcggggtcct tcatctgggt
     1621 ggtgtactca ctctcgggca cgttgcggcg cttgcggcgc ttctcatact ggtcggtgat
     1681 gcggcggttt tccttggaga tacgccgcga ctctttggcg gaaagatatc cgtcctgatt
     1741 tttagccatc gtgtgtttct ccttccaatc agcgtttcat cttggggtca aacgcatcgc
     1801 gcagaccgtc gcccacaaag ttgaagccca gaacggtcag cagcaggcag ataccggcgg
     1861 ggatccagat gaacaggtag ttcgtcataa cgtaggcgtt gttgacatcg ttgatgatgt
     1921 tgccccagga ggcgaacgga tacttgacgc ccaggcccag gaaggacagg gtggcctcgg
     1981 tcaggatggt ggagcccagg cccatcgtgc aggtaacgat aagctgcggg ataacgttgg
     2041 ggatcaggtg gcggaaaatg cggtgccaga ccttgatgcc gcaggcctcg gtggccagca
     2101 tgaactcctg ctcacgcagg gagaggatct ggccgcgtac caggcgggca atgctgggcc
     2161 agcccaggaa gccgagaatc agcatcaggt acagcatacg tttccaggaa tccacacgca
     2221 tggcgtccat ggccgcgccg atgatgatga tgaccggcat c
//
LOCUS       DBMDSQ010000325         2255 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_10532_length_2255_cov_2.209842, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000325 DBMDSQ010000000
VERSION     DBMDSQ010000325.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 2255)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2255)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2255
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10532_length_2255_cov_2.209842"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            136..1689
                     /locus_tag="ACZPAX_09555"
     CDS             136..1689
                     /locus_tag="ACZPAX_09555"
                     /inference="COORDINATES: protein motif:HMM:NF014895.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative PEP-binding protein"
                     /protein_id="HIR1114182.1"
                     /translation="MHTYTGVPASPGIVAGPVEQIDHGTTGLHRIVCDPFRERALYDV
                     AVVLAKDELRRLSQRAKGPDADILLFQIALLEDESFTNEIGDYIAAGAGGAAAVERAE
                     QIFANRLKNVDDEYIRERSVDVCDVCRRVVDILDGRPRRRLHLKRPSILVADRFFPSD
                     LFSLDRRMVLGLASDKDSTVSHAAIMARSMGIPAVVQLGEGASAMAAGRRAILDADSG
                     TLVVEPDGAQIAQADCKIALSRLHGAPPDPADTLPCLTKNGTAFRLMPTCNLSDNDLP
                     HTHSAAGVGIVRTETALLYDQSTATQTTRLRELLKNAEGVPLLVRSCDTRADDDAPWT
                     GEMQACMKGHRLFKPQIKAMLQAAPDGDLRVVFPMITDVSDWDDCLREVQDCRDELLE
                     AGCETGPLTMGCVVDMPSAALMAGDLMDHGAQLLAVDIEDLTRYTLGLVQDPTAAIRQ
                     LANPAVLRLVQHILEEAQARGMQVYLCGITVAAVSAMPAYLKLGVRTFSAEPAAIMPL
                     KRLLMEQEV"
     gene            complement(1969..2235)
                     /gene="rpsT"
                     /locus_tag="ACZPAX_09560"
     CDS             complement(1969..2235)
                     /gene="rpsT"
                     /locus_tag="ACZPAX_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HIR1114183.1"
                     /translation="MPNIKSQKDRVVQAKKEALHNKTIKSNLKTVVKKANAAIDSNAA
                     DKETVVKVAVSAIDSAKSKGVIHKNTAARKISRMAKRANKAAAQ"
BASE COUNT          424 a          693 c          702 g          436 t
ORIGIN      
        1 cagataatac tggatgtctt atcgagcatt ttggcaacgc agatgccgtg ccgcagccgc
       61 cggagcggtg cttaagccgt caggcgggaa ttgtgcggta tggcctaagg aaattcaata
      121 aaggaggcct actctatgca cacctacacc ggcgtgccgg cgtcgcccgg cattgtggcc
      181 ggcccggtcg aacagatcga ccacggaacc accggcctgc accgcatcgt atgcgacccc
      241 ttccgggaac gcgcactcta cgatgtggcc gttgtgctgg ccaaggacga actgcgccgt
      301 ttgagccagc gcgccaaagg ccccgacgcg gatatcctgc ttttccaaat cgcgctgttg
      361 gaggatgaat cctttaccaa cgaaatcggc gattacatag ccgccggtgc aggcggtgcc
      421 gccgccgtgg aacgcgccga gcagattttc gccaaccgct taaaaaatgt cgatgatgag
      481 tacatccggg agcgcagcgt cgatgtctgc gatgtctgcc gccgcgtggt agatatcctg
      541 gatggccgcc cgcgccgccg cctgcattta aagcggccca gcattctggt ggctgaccgg
      601 ttcttcccca gcgatttgtt cagcctggat cgccgcatgg tgctgggcct tgccagcgac
      661 aaggacagca cggtcagcca cgccgccatt atggcgcgca gcatgggcat ccccgctgtg
      721 gtgcagctgg gcgagggtgc ttctgccatg gcggccgggc ggcgggctat tctggacgcc
      781 gacagcggca cgctggtcgt ggagccggac ggcgcacaga tcgcccaggc tgactgtaaa
      841 atcgccctga gccgcctgca cggcgcaccg cccgacccgg cggataccct gccctgcctg
      901 acgaaaaacg gcacggcgtt ccgcttaatg cccacctgca acctgagcga taacgacctg
      961 ccccacaccc acagcgcggc gggcgtgggc attgtgcgca ccgagacggc gcttctgtat
     1021 gaccagagca cggccacaca gaccacccgc ctgcgcgagc tgctgaaaaa cgccgagggg
     1081 gtccccctgc tggtgcgcag ctgcgatacc cgcgccgacg acgacgcccc ctggaccggg
     1141 gagatgcagg cttgtatgaa aggccaccgg ctgttcaagc cgcagatcaa ggccatgctg
     1201 caggctgccc cggacggcga tctgcgggtg gtattcccga tgatcaccga cgtgagcgac
     1261 tgggacgact gtctgcgcga ggtgcaggac tgccgcgatg agctgctgga agcgggctgc
     1321 gagaccggcc cactgacgat gggctgcgtg gtggatatgc ctagtgccgc cctgatggcg
     1381 ggcgacctga tggaccacgg tgcgcagctg ctggccgtgg atatcgagga cctgacccgc
     1441 tacacgctgg gccttgtgca ggaccccacc gctgccatca ggcagctggc aaaccccgcc
     1501 gtactgcggc tggtgcagca tattctggag gaggcccagg cccgcgggat gcaggtgtac
     1561 ttatgtggca tcaccgtggc cgccgtcagc gccatgcccg cgtatttgaa gctcggtgtg
     1621 cgcaccttca gcgccgaacc ggcggccatt atgccgctga aacgactgct gatggagcag
     1681 gaagtatgac cacaaaaaag taggggaagg atttgtccct cccacagcgt gcgccatgat
     1741 tacgatgtta aatcgcagca gagttgtagg ggccgcatat atgcggcccg cgcgtgtgcg
     1801 gcagatggcc gcctgcgggc ggcatatatg ccgcccctac ccagtggcgt tgtttttgcc
     1861 gcgtgcctgc aacaaggtcc ccgggaggga taaatctctc ccctacacac atttaataaa
     1921 tgaaaatgcc cgccgtattt ctacggcggg cattgttcat gcgatcaatc actgagcggc
     1981 agccttgttg gcgcgcttag ccatgcggct gatcttgcgg gcagcggtgt tcttatggat
     2041 gacacccttg ctcttagcag agtcgatggc agagacagcg accttaacga cggtctcctt
     2101 gtcagcagca ttgctgtcga tggcagcgtt tgccttcttg accactgttt tcaggttgga
     2161 cttgatggtc ttgttgtgca gagcctcttt cttggcctga acaacgcgat ccttctgaga
     2221 tttaatatta ggcattcgtt ccacctcctt ggttc
//
LOCUS       DBMDSQ010000326         2250 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_10571_length_2250_cov_1.292694, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000326 DBMDSQ010000000
VERSION     DBMDSQ010000326.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 2250)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2250)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2250
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10571_length_2250_cov_1.292694"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(201..686)
                     /gene="rlmH"
                     /locus_tag="ACZPAX_09565"
     CDS             complement(201..686)
                     /gene="rlmH"
                     /locus_tag="ACZPAX_09565"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005922107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HIR1114184.1"
                     /translation="MQNIDLIAIGKMNAAYFAQGVAEYQKRLGGFCNFRIIELPEVQI
                     ADKNASDKQIAKALQKEGEAILASVRKGAYLVALCVEGRQVSSEDLAAMIAERAMSGA
                     GDMAFVIGSSHGLDDSVKRAAQARISLGRITLPHQLARLVLTEQLYRACTINAGMKYH
                     K"
     gene            complement(700..1488)
                     /locus_tag="ACZPAX_09570"
     CDS             complement(700..1488)
                     /locus_tag="ACZPAX_09570"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005925559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR1114185.1"
                     /translation="MAYFTTLYSGSSGNCGLVRCGDQYLLIDMGKSCRTTLTALKSLG
                     LAVSDCAGILITHEHSDHVAGLDTFLKKYPTPLYGCADTLDTLQSRGTIPPTVDAIPV
                     DGQTLEIGEFTVTSFPTSHDVPCCGYRVRTTDGHVMALATDLGYLTPVVQDNLIGCDL
                     VALEANYDAFSLHGGPYPYYLKVRIASDRGHLDNKACAAEILDLIQDGCKKFALCHLS
                     QTNNSPELALTTVYNVLLAAGIQPGRDVLIQAQHRSEVSPYIEF"
     gene            complement(1546..>2250)
                     /locus_tag="ACZPAX_09575"
     CDS             complement(1546..>2250)
                     /locus_tag="ACZPAX_09575"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HIR1114186.1"
                     /translation="DVANFLDSMGANIKGAGTDVIRIKGVEKLHRTEYSIIPDQIEAG
                     SYMVAAAITKGNITLHNVIPKHMEPITAKLRAAGCEVEEFDDALRITRTGELRPLKLK
                     TMPHPGFPTDMQPLMTALLTLAKGTSIVTEGIWENRFRYVDELIHMGANIQVDGQVAV
                     IEGVKELHPAPLRATDLRAGAAMVMAALAADGTSEVDETIHIERGYENIVEKLQALGA
                     DIRRVEIPANAVSRAM"
BASE COUNT          444 a          648 c          644 g          514 t
ORIGIN      
        1 ttctgcttgg ggctacaccg atttttgtag actctgatcc caatacctac aatatggatt
       61 ctctcgacct gctgcactac taatttgtaa atagaaactg ccctgtggag tgaatgactc
      121 cacagggcag tttttgtttt ggggtggcgg ttggttttcg ggccgatgct tgtcatccgc
      181 ccgggacgtc gaggacgccc ctacttatgg tatttcatcc ctgcattgat ggtgcaggcg
      241 cgatagagct gctctgtcag gaccaggcga gccagttggt ggggcaatgt gatacgcccc
      301 agactgatgc gggcctgcgc ggcacgcttg acgctgtcgt ccaacccatg ggaggagccg
      361 atgacaaacg ccatatcccc tgcgccgctc atggcacgct cagcaatcat ggcagccagg
      421 tcctcgctgg acacctgcct gccctcgaca cagagggcaa caagatacgc ccccttgcgc
      481 acgctggcta aaatcgcctc tccctccttt tgcagggctt tggcaatctg cttatcgctg
      541 gcgtttttgt cggcaatctg cacctccggc agctcgataa tgcggaaatt gcagaaacca
      601 cccagccgct tctgatattc ggctaccccc tgcgcgaagt aggcggcgtt catcttgccg
      661 atggcaatca aatcaatatt ctgcatggtc tttccccgct taaaattcaa tgtaggggct
      721 gacttcgctg cggtgctgcg cctgaatcag cacatcccgc ccgggctgga tgcccgctgc
      781 cagcagcacg ttatagaccg tagtcaacgc cagctccggc gagttgttcg tctggctcaa
      841 atggcagagg gcaaactttt tgcagccgtc ctggatcagg tcaagaattt ccgccgcgca
      901 ggccttgttg tccaagtggc cccggtcgct ggcaatgcgg actttcaagt agtagggata
      961 cggcccgccg tgcaggctga aagcgtcgta gttggcctcc agcgctacca ggtcacaccc
     1021 gatcaggtta tcctgtacga ctggcgttag atagccaagg tcagtggcaa gggccatcac
     1081 atggccgtcc gttgtgcgga cgcggtaacc gcagcagggc acgtcatggc tggtgggaaa
     1141 agatgtcacg gtaaactcac caatttcaag cgtctggccg tccaccggaa ttgcatccac
     1201 cgtaggcggg atggttcccc tggattgcag ggtatccagc gtatccgcac agccgtacag
     1261 cggggtggga tattttttca ggaaggtgtc cagtccggcg acgtggtcgg agtgctcgtg
     1321 cgtgatgagg atgccagcac agtcgctgac ggccagtccc agggatttca gcgcagtcag
     1381 agttgtgcgg cagcttttgc ccatatcgat gagcagatat tggtcgccgc agcgcaccag
     1441 cccacagttg ccggaggagc cagaatacaa ggtggtaaaa tatgccattt gtctgtccta
     1501 acgctttcca taaaaattgg gccgcctaag cggcccagga gttttttaca ttgcccggct
     1561 cacggcattg gccggaattt ccacacgacg gatatctgcg cccagggcct gcagtttctc
     1621 cacaatgttc tcatagccgc gctcgatgtg gatggtctca tccacttcgc ttgtgccgtc
     1681 ggctgccagc gcagccatga ccatggcggc accggcacgc aggtcagtag cccgcagcgg
     1741 cgcggggtgc agttccttga cgccctcaat gacggcgacc tggccatcca cctggatgtt
     1801 tgcacccatg tggatcaact catccacata gcggaaacgg ttttcccaga tgccctcggt
     1861 cacgatggaa gtacctttgg caagggtcaa cagcgccgtc atcagcggct gcatatccgt
     1921 cggaaagccg gggtgcggca tcgtcttgag tttcagtgga cgtagctcac cggtgcgggt
     1981 gatgcgcagg gcatcgtcaa attcctccac ctcgcagcct gcggcacgaa gcttggctgt
     2041 gataggctcc atatgcttgg ggatgacgtt gtgcagcgtg atattgccct tggtgatggc
     2101 ggcggctacc atgtagctgc ctgcctcgat ctggtccgga atgatggaat actctgtgcg
     2161 gtgaagcttc tcaacaccct tgatacggat cacatctgta ccagcacctt tgatattggc
     2221 tcccatgctg tccaggaagt tggcaacatc
//
LOCUS       DBMDSQ010000327         2246 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_10594_length_2246_cov_1.752448, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000327 DBMDSQ010000000
VERSION     DBMDSQ010000327.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 2246)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2246)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2246
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10594_length_2246_cov_1.752448"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1498)
                     /locus_tag="ACZPAX_09580"
     CDS             complement(<1..1498)
                     /locus_tag="ACZPAX_09580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1114187.1"
                     /translation="MTAQNARLRRCGAALLCLVCAAVMVRSAFVGLEIDEEYALSLGF
                     RLVSGDRLFYSMWEPHQLSSLPSAALLAAFMAVTGGTTGVLLFFRLVVLVCKAAMSYA
                     FYREFRRDLGGRGALLAALVLFAFVPKWFLGPDYTGQQFHWTVAAFLCLHHYVSRGFQ
                     KPWLVAVGAVCACFGYLAFPQSIAAFAVLWVGLFVLGRRGGERCRWGIPRGVWLLTGS
                     CAGCGAAFLLYALWGVGFDPALLAARLQLILHDPQYDFTTGARLAALAAQAWSVAKSL
                     VWPLAAAALVCALMWALRLRGVGDAALYDKGDALCRVLSLWAALAALQCLVRAVADRS
                     LDERQFVPVLVLAGAAAFRKGRGTPGSAALFWLGYLPGLAAYAMILRSTLLGLAPTFM
                     YLMWPAVCGALAMLNARDKCGAARRAQGAICLAEALAFLLLCRVWCVEVTGWKPASVA
                     DTPLVRIESGPAKGIYADEKAADMQECLVEALEPYAGQAVLQAIGEQHG"
     gene            complement(1495..>2246)
                     /locus_tag="ACZPAX_09585"
     CDS             complement(1495..>2246)
                     /locus_tag="ACZPAX_09585"
                     /inference="COORDINATES: protein motif:HMM:NF015379.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1114188.1"
                     /translation="CEKEYFVFTDAPAIEYEDAPRVHRIYQEAYPWPYSTLKRFSIFL
                     TQEDALRGMDYLFFFNANLTCKKTVTAEEFLPRPEKGENLLLVQQPGFWNKKPPFYTY
                     DRNPKSTAYIPYNCGKDYVSGGLNGGTADAFLVLCHELERRTEQDLQNGVIALWHDES
                     QLNRYAAERSDYRLLTPAYWYPEGWDMPFEEKISVRDKARYFDVAAVKNKETKQRGFL
                     ARKWGAFCENYLPCWYCLRDTLLHKTLGAGR"
BASE COUNT          462 a          808 c          637 g          339 t
ORIGIN      
        1 ggccgtgctg ctcgccgatg gcctgcaata cggcctgccc tgcgtagggt tccagcgcct
       61 caaccaggca ctcctgcata tcggcggcct tctcgtcggc gtaaatgccc tttgcggggc
      121 cgctctcaat gcgcaccagc ggcgtgtcgg ccacactggc gggcttccag ccggtcacct
      181 ccacgcacca gacgcggcag agcagcaaaa acgccagtgc ctctgccagg caaatagccc
      241 cctgcgcacg gcgggccgcg ccgcacttgt ctctcgcgtt cagcattgcc agcgcaccgc
      301 agacagcggg ccacatcagg tacataaagg tcggggccag gcccagcagc gtcgagcgca
      361 ggatcattgc gtaggccgcc agcccgggca ggtaacccag ccagaacagc gccgcgctgc
      421 ccggcgtgcc gcggcctttc cggaacgctg ccgcgcccgc cagcaccagc accggcacaa
      481 actgtcgttc gtcaaggctg cggtccgcca ccgcacgcac cagacattgc agtgccgcca
      541 gtgccgccca caggctcagc acccggcaca aagcatcccc tttgtcatag agggcggcgt
      601 ccccgacgcc ccgcaaacgg agcgcccaca tcaaagcaca gacgagtgcc gctgccgcca
      661 acggccacac cagcgatttt gccacgctcc acgcctgggc ggccagcgcc gccagccgcg
      721 caccggtggt aaaatcatac tgtgggtcgt gtaaaatcag ctgcaaacgc gccgctagca
      781 gcgccgggtc aaacccaacg ccccacagcg catagagcaa aaatgccgcg ccgcacccgg
      841 cacagctgcc ggtcagcagc cagacgccgc ggggaatgcc ccagcggcag cgctcgccgc
      901 cgcgccgccc cagcacaaac agccccaccc acagcaccgc aaacgccgca atactctggg
      961 ggaacgccag atagccgaag caggcgcaga ccgctcccac cgccacgagc cacggttttt
     1021 gaaacccgcg gctcacataa tggtgcaggc acaaaaacgc cgccacagtc caatggaact
     1081 gctggccggt gtaatccggc cccaaaaacc acttgggcac aaaggcaaac agcaccagcg
     1141 ccgccagcag cgccccgcgc ccgcctagat cccggcggaa ctccctgtaa aacgcatagc
     1201 tcatcgcggc cttgcacacc agcaccacaa gccggaaaaa cagtagcacg ccggtcgtgc
     1261 cgccggtcac agccataaac gccgccagca gcgccgccga cggcaggctg gaaagctggt
     1321 gcggctccca catcgagtag aacagcctgt ccccgctgac gagccggaac cccagcgaca
     1381 gcgcgtactc ttcgtcgatt tccaacccca caaaggcgct gcgcaccatg accgccgcac
     1441 acaccaggca gagcagggcc gcaccgcacc ggcgcagccg cgcgttctgc gccgtcatcg
     1501 gccggccccc agcgttttgt gcagcagggt atcccgcagg cagtaccagc agggcaggta
     1561 attttcgcag aaggcgcccc attttcgcgc caggaatccg cgctgcttcg tttctttgtt
     1621 tttcaccgcc gccacatcaa agtagcgggc cttgtcccgc accgatatct tttcctcaaa
     1681 cggcatatcc cagccctcgg gataccagta ggccggggtc agcaggcggt aatcgctgcg
     1741 ctcagccgcg tagcggttca gctggctttc gtcatgccac agggcgatca caccgttctg
     1801 caagtcctgc tcggtgcggc gttccagctc atggcacagc accagaaacg catccgccgt
     1861 gccgccgttc agcccgccgg agacgtagtc cttcccgcag ttgtagggga tgtaggccgt
     1921 gctcttgggg ttgcggtcgt aggtataaaa cggcggcttt ttgttccaga agcccggctg
     1981 ctgcacgagc agcagatttt cgcccttctc gggccggggc agaaattcct ccgctgtaac
     2041 ggtctttttg caggtaagat tcgcattaaa gaaaaagaga taatccatcc cccgcagcgc
     2101 atcctcctgc gtcagaaaga tggagaaccg cttcagtgtg ctgtacggcc aggggtaggc
     2161 ctcctggtaa atgcggtgta cgcggggcgc gtcctcatat tcaatggcag gggcatccgt
     2221 aaagacaaag tattccttct cgcacc
//
LOCUS       DBMDSQ010000328         2238 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_10654_length_2238_cov_2.303697, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000328 DBMDSQ010000000
VERSION     DBMDSQ010000328.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 2238)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2238)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2238
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10654_length_2238_cov_2.303697"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..>2238
                     /locus_tag="ACZPAX_09590"
     CDS             <1..>2238
                     /locus_tag="ACZPAX_09590"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sortase family protein"
                     /protein_id="HIR1114189.1"
                     /translation="YILSRVFDHQESYDAALAHSEAVFDFGTESYADPQKARRIAVPE
                     QLLKRFPGDVVQAAAGRALAAMQVTGADYAAAYMPASVGQCPFVLVCDRRGAVACALP
                     PDMNDTFIANQILDLLRRRLFGLQLTDSCITFRPGHDRPLLIVSEAAKSRGNTVRFSL
                     RRRTPPTRDADHTADFEPMLDFDRPVAPPKFAAPAQEVEAAAPETDSAPQHRTLRRHE
                     AAPTPPPAAPAVPDHEPTGVIQFESDDAPAPAMPDPLAVQTAGPENARHARIRRARPV
                     TADPAPSVPLMPVDLPDFSENPPETEPQAAPRSVLDDDIPDFSADLDPAAIAAAQAAD
                     DAAEAAGKSASVEDFTRAATQLFDASAIEEAAREKAKPRKKRKKNADSDADLPPKQPA
                     PPSIKNRSLEIIEKSERRRRRTVTLILVVLLLAVLAGAGALWVFFRNDLGASPAAKSY
                     GTALYDSTAESYLDKALDRRAGVVAYLGWPGFDGALVYSADTPTPETPESGAEPGSIV
                     RFATPSALDAATPGNTVLECTGDDYKALADQDTLKQNGGFTLYTADRTYRFKALAVYY
                     CDPAEQGEGAFDLYGSTDLSNYYDYLTFVAGIQARSLFDTGVEVGDESHFLTVTTQSG
                     ENGALLCITGRLVEDGEAEVLNTAAFTATEEPLLTAAQYQSKGQPMPTVSALMRTSVE
                     RYAQQSAATQAARKSSGTARDDTIAAADLAQRADELQNITDSLLASTDKMLKGLTDVA
                     GSTNAV"
BASE COUNT          392 a          818 c          702 g          326 t
ORIGIN      
        1 tacatccttt cgcgggtgtt cgaccaccag gagtcctacg acgcggcgct ggcccacagc
       61 gaggccgtgt tcgactttgg caccgagagc tatgccgacc cgcagaaagc ccgccgcatc
      121 gccgtgccgg aacagctgtt gaagcggttc cccggcgatg tggtgcaggc cgccgcaggc
      181 cgcgccctgg ccgccatgca ggtgaccggc gcggactacg cggctgccta tatgcccgcc
      241 agcgtgggcc agtgcccctt tgtgctggtg tgcgaccgcc gcggcgcggt ggcctgcgcc
      301 ctgccgcccg atatgaacga cacgttcatc gccaaccaga ttttggatct gctgcgtcgc
      361 cgcctgttcg gcttgcagct gaccgactcc tgcatcacct tccgccccgg gcacgaccgg
      421 ccgctgctta tcgtgtcgga ggccgccaag agccgcggca atacggtccg attctcgctg
      481 cgccgccgca cgccgcccac ccgcgacgcc gaccatacgg cggattttga gccgatgctg
      541 gactttgacc gcccagtggc cccgccgaaa tttgccgccc ctgcgcagga agtggaagca
      601 gccgcgccgg agacggactc cgccccgcag caccgcacgc tgcgccgcca cgaagccgcg
      661 cccaccccgc cgcccgcggc cccggctgtg cctgaccatg agccgaccgg tgtcatccaa
      721 ttcgagagcg acgacgcccc ggccccggcg atgcctgacc cgctggcagt ccagacggct
      781 ggccccgaaa atgcccgcca tgcccgcatc cgccgcgccc ggcctgtgac cgctgacccg
      841 gcccccagcg tgccgctgat gccggtagac ctgccggact tttcggaaaa tccgccggag
      901 accgagccgc aggctgcgcc gcgctcggtg ctggacgatg atatccccga tttctccgct
      961 gaccttgacc ccgccgccat tgccgctgcc caggccgccg atgacgccgc cgaggccgca
     1021 ggcaagagcg ccagcgttga ggactttacc cgcgcggcga cccagctgtt tgacgccagc
     1081 gccattgagg aggctgcccg ggaaaaagca aagccccgca aaaagcgcaa aaagaacgca
     1141 gattcggacg ccgacctgcc cccgaagcag cccgcaccgc ccagcatcaa aaaccgcagt
     1201 ttggaaatta ttgaaaagag cgagcgccgc cgcagacgca cggtcacgct gatcctggtc
     1261 gtgctgctgc tggctgtgct ggccggtgcc ggggcactgt gggtgttctt ccgcaatgat
     1321 ctgggtgcca gccctgccgc caagagctac ggcacggcgc tgtacgattc caccgccgag
     1381 agctatcttg acaaggcgct ggaccgccgc gcaggcgtgg tggcctacct gggctggccc
     1441 ggctttgatg gcgcgttggt ctactctgcc gacacgccca cgcccgaaac gccggaatcc
     1501 ggcgcagagc cggggtccat cgtccgtttt gcaaccccca gcgccctgga cgccgccacc
     1561 cccggcaaca ccgtgttgga atgcaccggt gacgactaca aggcgctggc cgaccaggac
     1621 acactgaaac agaacggcgg cttcacgctg tatacggcag accgcaccta ccgcttcaaa
     1681 gcgctggcgg tctactactg cgatcccgcc gagcagggcg aaggtgcgtt tgacctgtat
     1741 ggcagcaccg acctgtccaa ctattacgac tacctgacct ttgtggcggg catccaggcc
     1801 cgcagcctgt ttgacaccgg ggtggaggtg ggtgacgagt cccacttctt gaccgtgacg
     1861 acccagagcg gcgagaacgg tgcgctgctc tgcatcacgg gccgcctggt cgaggacggc
     1921 gaggccgagg tgctgaacac cgctgccttt accgccacag aggagccgct gctgacggcg
     1981 gcccagtacc agagcaaggg ccagccgatg cccacggtca gcgccctgat gcggaccagc
     2041 gtggagcgct atgcccagca gagcgccgcc acccaggccg cccgcaagag cagcggcacg
     2101 gcccgggacg acaccatcgc cgccgccgat ctggcccagc gcgccgacga attgcagaac
     2161 atcaccgaca gcctgctggc cagcaccgac aagatgctca agggcctgac cgatgtggca
     2221 ggctcgacga acgctgtg
//
LOCUS       DBMDSQ010000329         2229 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_10711_length_2229_cov_2.155545, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000329 DBMDSQ010000000
VERSION     DBMDSQ010000329.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 2229)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2229)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2229
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10711_length_2229_cov_2.155545"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..630
                     /locus_tag="ACZPAX_09595"
     CDS             <1..630
                     /locus_tag="ACZPAX_09595"
                     /inference="COORDINATES: protein motif:HMM:NF013086.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HIR1114190.1"
                     /translation="IWGVAIDAATGARYASEALGRNPISTASLSAMAKGGAKPYIIWD
                     EKGLTKFHNMARVARSMNGLNGRNGTMYKFATLDEVAAFYNTDAAKLKATITEYNRMI
                     AAGKDEQFMKPLERSGRKVEPLSETGPFYTALMIPRWDYCPGGIATDTTARALRLADS
                     EPVPGLFVVGEAAGGIHGAERLTACSMPDCCVTGMIAGETAAKLQKRTI"
     gene            645..1037
                     /locus_tag="ACZPAX_09600"
     CDS             645..1037
                     /locus_tag="ACZPAX_09600"
                     /inference="COORDINATES: protein motif:HMM:NF025890.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HIR1114191.1"
                     /translation="MNIFKSAAIVLALAASLAVNAQPTTIKHKGLECTTCHVDGKLTP
                     PDAKTCLACHREESIVKAGERFNFESYFKDPRDGSEIRKEVAINPHDNFHYGRTDQCV
                     NCHREHRPSTVTCEKCHDIEPWKMKAPR"
     gene            1417..>2229
                     /locus_tag="ACZPAX_09605"
     CDS             1417..>2229
                     /locus_tag="ACZPAX_09605"
                     /inference="COORDINATES: protein motif:HMM:NF013086.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR1114192.1"
                     /translation="MSRHFFKLTTTALVVGLVSACAQAGVDPATLPVKNLKADVIVVG
                     AGGTGLAAAAAARLAGAEVLVIEKLPMVGGSSAFAGGAIAAGDSNAQKRVGSQGTSTE
                     EFVKIWLNDQKRSSAGGDPSYPDERLVRAMAREFTVTANWLEDKIGHKYAPPRPFGYG
                     GPDFAHAPAESPVPASGRGSSPAGGRFVIQNLMKFLDAHQVPVMKGTPADDLILNEKG
                     DVVGVKAHNGKLRYEISGKAVVLATGGFPHNRAMLEKLVPDYAPYADLSVASI"
BASE COUNT          442 a          667 c          657 g          463 t
ORIGIN      
        1 atttggggcg ttgcgatcga tgccgctacg ggtgctcgct atgccagcga ggctcttggc
       61 cgcaatccga tttcgacggc ttctctctcc gccatggcca aggggggtgc caagccctac
      121 atcatctggg atgaaaaggg cctgacaaag ttccacaaca tggctcgagt tgcacgaagc
      181 atgaacggtc tgaacggtcg caacggcacg atgtacaagt tcgcgacgct cgacgaggtt
      241 gcggctttct acaacaccga tgccgccaag ctcaaggcca cgatcaccga atacaaccgc
      301 atgatcgctg caggcaagga tgaacagttc atgaagcctc ttgagcgttc aggacgaaag
      361 gttgaaccgc tttccgaaac gggtccgttc tataccgccc tgatgattcc tcgctgggac
      421 tactgcccgg gcggcatcgc gaccgacacg acggctcgtg ccctgaggct ggctgacagc
      481 gagcccgttc cgggtctttt cgtggtggga gaggccgctg gtggcattca cggtgctgaa
      541 cgacttacgg cctgctcgat gcctgactgc tgcgttaccg gcatgattgc cggcgaaacg
      601 gccgccaaac tgcaaaagcg aaccatttaa gggagacagc aatcatgaac atcttcaaat
      661 ccgccgctat cgtgctggcg ttggctgcct ctctcgccgt caatgctcag cctacgacca
      721 tcaagcacaa ggggctcgag tgcacgacct gccatgtcga cggcaagctt acgccgcccg
      781 acgccaagac ttgccttgcg tgccaccgcg aagagtccat cgtcaaggct ggcgagcgct
      841 tcaacttcga gtcctatttc aaggacccgc gcgacggatc ggaaatccgc aaggaagtcg
      901 ccatcaaccc gcatgacaac ttccactacg gccgtaccga tcagtgcgtc aactgccatc
      961 gcgaacatcg tccgagcacc gtcacctgcg aaaagtgcca cgacatcgaa ccttggaaga
     1021 tgaaggcgcc gcgttaagcg tgttgagcac ctatagtgcg acatgaaaac cgcccgcgtt
     1081 gtgaacagcg cgggcggttg ttgttttatc gacggccagt gtgtcgggga gggcaactct
     1141 tgtcttttcc cctgacctgt cgggtcaggt caatgccgcc gattttgagg tcggggcgat
     1201 taatgggcgc caactgtcca tcagcttgag attgtgattg agcgccttgc ctctgggctt
     1261 ctggaacgcc agcttcttgg aaaagacctc gatgggcggc ttgcgctgca ggcagtacgg
     1321 atgctgccat agggcatgtc cgcattgatg gggttcgcgc tccgaactag acttcctctg
     1381 tctcttataa acatcccgaa caggggagaa accctcgtgt cccgacattt tttcaagctt
     1441 acaaccacag ctcttgtcgt cggtcttgtc agtgcctgcg ctcaggcggg cgttgatccc
     1501 gcaacgcttc ccgtcaaaaa cctcaaggcc gacgtgatcg tcgtcggtgc gggtggtacg
     1561 ggtctggccg ctgctgcggc cgcgcgcctt gccggcgccg aggtgcttgt cattgaaaag
     1621 ctcccgatgg tgggcggcag ctctgctttt gcgggtgggg ccattgctgc tggagattcc
     1681 aatgctcaga agcgagttgg aagccagggg acctcgaccg aggagtttgt caagatctgg
     1741 ctcaatgacc agaagcgctc ctccgcgggc ggcgatccct cctatcctga tgagcgtctt
     1801 gtgcgcgcca tggccagaga gtttacggtg actgccaact ggctcgaaga caagatcggc
     1861 cacaagtatg cgcccccgcg tccatttggc tacggtgggc ccgattttgc gcatgcgcct
     1921 gctgaatccc ccgtacccgc atccggccgc ggttcctctc ctgccggcgg caggttcgtg
     1981 atccagaacc tgatgaagtt ccttgatgcc catcaggtgc ccgtcatgaa ggggacgcct
     2041 gcggacgacc tcattctcaa tgaaaagggc gacgtagtcg gcgtcaaggc gcacaacggc
     2101 aagcttcgct atgaaatttc gggcaaggcg gtcgtgctcg ccacgggcgg cttcccgcac
     2161 aacagggcga tgcttgagaa gctggtgccc gactatgctc cctatgccga cctgtccgtt
     2221 gcgagcatt
//
LOCUS       DBMDSQ010000330         2229 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_10712_length_2229_cov_1.742481, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000330 DBMDSQ010000000
VERSION     DBMDSQ010000330.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 2229)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2229)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2229
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10712_length_2229_cov_1.742481"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(152..1546)
                     /locus_tag="ACZPAX_09610"
     CDS             complement(152..1546)
                     /locus_tag="ACZPAX_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HIR1114193.1"
                     /translation="MRYSKHLTYSEHMFLRSRLNRVRHITLDPDGPGVVRIHIVPCHK
                     PDHETPFTVILNGQDILPLNVSWAILLTNLIEALKPCTGKEIRPEEWAAINAQAVAAT
                     RKIYRKTEYAQIESDLQLLVDSLCTIARGGEPPLTVQPMSLAEYAPRMVAPHRMDLMV
                     SSMVKDGAWHCNQKCLHCYAANQPLSAVPELDTDQWLAVIEKCRNAGIPQLTFTGGEP
                     TLRHDLVKLVQAAQWFVTRLNTNGRMLTSMMCKELHEASLDAVQITFYSADADIHNEL
                     VGVDGYNDTLNGIKNALAAGLNVSLNTPLCSLNRDYLSTVKLAHELGIRYLTCSGLIP
                     AGNAETAASRAVRLTPEELEATLRPAMEYAAANGLEINFTSPGWLSEDTLRGLGFTQI
                     PSCGACLSNMAIAPDGTVLPCQSWLTGKGLGNLLRTPWQRIWRSDECRAIRGQSAKME
                     RRCQLGETPMQEGC"
     gene            complement(1818..>2229)
                     /locus_tag="ACZPAX_09615"
     CDS             complement(1818..>2229)
                     /locus_tag="ACZPAX_09615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HIR1114194.1"
                     /translation="TSMPVGRALMVSDVGIVLVGAYLYGPATGLYCILGMILKSTVAD
                     SAIESINMRKVCTIVTSNPTPVRDFIVNDLHRSATMEQAEGAYTHDEKWVLTTVLTRG
                     QAQKLRLYVRGLDDHAFITIVNSSEIVGKGFRAI"
BASE COUNT          415 a          671 c          681 g          462 t
ORIGIN      
        1 acggtcacga tgtagctgcg gatataatcc atgtcgccgc tctcggcgta gggggcgtcc
       61 acctcggccg ccgcagcggg cagagccagc agactgccgc acagcaggac ggcccacaaa
      121 agcgccagac ggcgcagaac actcgttttc atcagcagcc ctcctgcatc ggggtctctc
      181 ccagctggca gcggcgctcc attttggcgc tctgcccgcg gatggcgcgg cattcgtcgc
      241 tgcgccagat gcgctgccag ggtgtgcgca gcaggttccc caggcccttg ccggtcagcc
      301 agctctggca gggcagcacg gtgccgtccg gcgcaatcgc catgttggac agacaggccc
      361 cacagctggg gatctgggta aagcctaaac cgcgcagcgt gtcctcgctg agccagccgg
      421 ggctggtaaa gttgatttcc agaccgtttg cggcggcgta ttccatggcg gggcgcagcg
      481 ttgcttccag ctcctccggc gtcaggcgca cggcgcggct ggcggcagtt tcggcgttgc
      541 ccgcagggat cagcccgctg caggtcaggt agcggatgcc cagctcgtgg gcaagtttga
      601 ccgtggacag ataatcccgg ttcaggctgc acagcggagt gttcaggctc acattcagcc
      661 cggcggccag cgcgtttttg atgccgttca acgtatcatt gtagccgtcc acgcccacca
      721 gctcattgtg gatatcggcg tcggcggagt agaaggtaat ctgcacggcg tcgagactgg
      781 cctcgtggag ttccttgcac atcatgctgg tcagcatacg gccgttggtg ttcagccggg
      841 tgacgaacca ctgcgcagcc tgcaccagct tgaccaaatc atggcgcagc gtcggctcgc
      901 cgccggtaaa ggtcagctgc gggatacccg cgttgcggca tttttcaatc acggccagcc
      961 attggtctgt gtccagctcc ggcacggcgg aaagcggctg gtttgcagcg tagcagtgca
     1021 ggcatttctg gttgcagtgc cacgcgccgt ccttgaccat cgagctgacc atcagatcca
     1081 tgcggtgggg ggccaccatg cggggcgcgt actcggccag gctcatgggc tgcacggtca
     1141 gcggcggctc gccgcccctg gcaatcgtac acaggctgtc caccagcagc tgcaaatcgc
     1201 tctcgatctg cgcatactcg gtcttgcggt aaattttgcg ggtggccgcc acagcttggg
     1261 cgttgatggc cgcccattct tcaggacgta tttctttgcc ggtgcagggc tttaaagcct
     1321 cgatgaggtt ggtcagcaga atggcccagc tgacgttcag cggcagaata tcctggccgt
     1381 tcaaaatcac ggtgaacggc gtctcgtggt cgggcttatg gcagggtacg atatggatgc
     1441 gcaccacacc ggggccgtcc gggtccagtg tgatatgccg cacccggttc agccggctgc
     1501 gcaggaacat atgttccgaa taggtcaaat gttttgaata tctcataaaa agaggttccc
     1561 ctcgttcaag tttcttatac aggttgctta cgatacgtat aaaaagtata gcatatttcg
     1621 ggggtgatta caacctggtt acaatagaaa aagcacccat ccgaagatag gtgcttccta
     1681 aaaggcttcc ccctaagggg aagcggactg atgagggaca ggtctgccct attgaccgtt
     1741 ttcggtttct tgcgggcagc tcacccctca tccggccctg cggggccgct ttctcccagg
     1801 ggagaaggct ttggagttca aatcgcccta aaccccttgc ctacgatctc gctgctgttg
     1861 acgatggtta taaacgcgtg gtcgtccagg ccgcgcacat acaggcgcag cttctgcgcc
     1921 tggccgcggg tcaacacggt ggtcagcacc cacttttcgt cgtgggtgta ggcaccctcg
     1981 gcctgctcca tcgtagccga tcggtgcagg tcgttcacga taaaatcacg caccggcgtt
     2041 gggtttgacg tcacgatcgt gcagaccttg cgcatattga tactctcaat cgcgctgtca
     2101 gcgacggtgc tctttaaaat catgcccaga atgcagtaca gcccggtggc ggggccgtag
     2161 agatacgcgc ccaccagcac aatgcccaca tcgctgacca tcagcgcacg gccgaccggc
     2221 atactggtg
//
LOCUS       DBMDSQ010000331         2201 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_10902_length_2201_cov_2.270476, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000331 DBMDSQ010000000
VERSION     DBMDSQ010000331.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 2201)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2201)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2201
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10902_length_2201_cov_2.270476"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1989)
                     /locus_tag="ACZPAX_09620"
     CDS             complement(<1..1989)
                     /locus_tag="ACZPAX_09620"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005925813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HIR1114195.1"
                     /translation="MKTVNQSMRKKDAMQLVTGQPVYMDDVIPQDCLIVKLLRSPHAN
                     AIVQEIDTSRALLVPGIEAIYTWKDVDQQGRRYTQAGQTYPEPSPYDRLVIDRHVRFA
                     GDVVAILAGKDEKCVDKAMKLIKVRYEVLPAVLDYHTALDNPVLVHPEENWESLAPVG
                     ADNKRNLCAHDESGAGDIEAVLAGCDVVIDHTYHTRACQQAMMETFRTYCSIDAYGRL
                     NVLSSTQIVFHCRRILANALHIPKSMIRVAKPRIGGGFGAKQTSVCEVYPAFVTWKTK
                     KPSKIIFSRYESQIASTPRHEMELHVRLGATKDGIVRGIDLYTLSNTGAYGEHGPTTV
                     GLSGHKSIPLYGKAEAFRFVSDVVYTNHMSAGAYRGYGATQGLFAVESAVNELADKLG
                     IDPFVIRQRNIVHEGDVMPAYYGQVNTSCALDRCLQAVHDNIGWDEKYPVRDMGNGKV
                     RAVGMGMAMQGSGITSVDVGSASLKINDDGFYTLSIGAADMGTGCDTILAQIAAEVLD
                     CPLDNVTVLGADTDSSPYDSGSYASSTTYVTGKAVEQCAEQLKQKICQVGAGLLGLDE
                     RAVVFAGDAVTSEDGTQRATLAQIAAASQCGSNTALEAVVTHSSEISPPPFMVGAAEV
                     EVDLETGEAQVIRYEAAVDCGTPVNPNLARVQAEGGILQ"
     gene            complement(1986..>2201)
                     /locus_tag="ACZPAX_09625"
     CDS             complement(1986..>2201)
                     /locus_tag="ACZPAX_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015538514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HIR1114196.1"
                     /translation="FIADQGAEQCGFCNPGFIMNALALFRENPDPGEDEIKEYLAGNL
                     CRCSGYEGQLRGILNFLAWKKGKEAAQ"
BASE COUNT          377 a          701 c          695 g          428 t
ORIGIN      
        1 ctgcaggatg ccaccctcgg cctgcacccg cgcgaggttc gggttgaccg gtgtgccgca
       61 gtcaacggcc gcctcatagc ggatgacctg cgcctcgccg gtttccagat cgacctcgac
      121 ctcggccgcg cccaccatga acggcggggg cgagatctcc gagctgtggg tcacgactgc
      181 ctccagcgcc gtattgctgc cgcactggga ggctgcggcg atctgcgcca gcgtggcgcg
      241 ctgcgtgccg tcctcgcttg tcacggcatc gcccgcaaag acgaccgcac gctcatcaag
      301 gcccagcagc cccgcgccga cctgacagat cttctgcttg agctgctcgg cgcactgctc
      361 aaccgccttg ccggtgacat aggtcgtgct ggatgcgtag gagccggaat catagggcga
      421 ggaatcggtg tccgcgccga ggaccgtcac attgtcgagc gggcagtcca gcacctcggc
      481 ggcaatctgc gccagaatcg tgtcgcagcc ggtgcccatg tcggccgcac cgatcgagag
      541 ggtatagaac ccgtcatcgt tgatcttcag gctggcgctg cccacatcca cactcgtaat
      601 gccggagccc tgcatcgcca tgcccatgcc cacggcgcgc accttgccgt tacccatgtc
      661 gcgcacgggg tatttttcgt cccagccgat gttgtcatgc acagcctgca ggcagcggtc
      721 cagcgcgcag ctggtgttga cctgcccgta gtaggcgggc atcacatcgc cctcatggac
      781 gatgttgcgc tggcggatca caaacgggtc gatgcccagc ttgtcggcca gctcattgac
      841 ggcggactcc accgcaaaca ggccctgggt cgcaccgtag ccgcggtaag cgcccgcgga
      901 catgtggttc gtgtagacga catcgctgac aaagcggaat gcctcggcct tgccgtagag
      961 cgggatggat ttgtggccgg acaggcccac cgttgtaggc ccatgctcgc cgtaagcacc
     1021 ggtgttggac agggtgtaga ggtcgatgcc cctgacgatg ccgtcctttg tcgcaccaag
     1081 gcggacatgc agctccatct catggcgcgg ggtggaggcg atctggctct cgtaccggct
     1141 gaagatgatc ttgctgggct ttttggtttt ccatgtcaca aaggcggggt agacctcgca
     1201 gaccgaggtc tgcttggcgc cgaagccgcc gccgatgcgg ggcttggcca cccggatcat
     1261 cgacttgggg atgtgcagcg cattggccag gatccggcgg cagtggaaaa cgatctgggt
     1321 cgagctgagg acattcagcc gcccgtaggc atctatgctg cagtaggtgc ggaaggtctc
     1381 catcatggcc tgctggcagg cgcgggtgtg gtatgtatgg tcgatgacga catcgcaccc
     1441 cgccagcacc gcctcaatat cgcccgcccc gctctcatcg tgggcgcaga ggttgcgctt
     1501 gttgtcggcc ccgaccggtg ccagcgactc ccagttttcc tccgggtgga caaggacggg
     1561 gttatccagc gccgtgtggt agtccagcac ggcgggcagg acctcatacc ggaccttgat
     1621 gagcttcatc gccttatcga cgcatttttc atccttgccg gccaaaattg cgacaacatc
     1681 ccccgcaaag cggacatggc ggtcgatgac aagccggtca taggggctcg gctcggggta
     1741 ggtctggccc gcctgggtgt agcggcggcc ctgctggtcc acatccttcc atgtgtagat
     1801 cgcctcgatg cccggcacca gcagggcacg gctcgtgtcg atctcctgca cgatagcgtt
     1861 ggcatggggg gagcgcagca gctttacgat caggcagtcc tgcgggatga catcatccat
     1921 gtagacgggc tggccggtca caagctgcat agcgtccttt ttgcgcatgg actggttcac
     1981 ggttttcatt gggcagcctc cttccccttt ttccacgcta aaaagttcaa aatgccccgc
     2041 agctggccct catagccgga gcagcggcag aggttgccgg ccagatactc cttgatctcg
     2101 tcctcgccgg ggtcggggtt ttcccggaaa agcgccagcg cgttcataat aaagcccggg
     2161 ttgcagaagc cgcactgctc cgcaccctga tcggcgataa a
//
LOCUS       DBMDSQ010000332         2194 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_10943_length_2194_cov_2.223125, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000332 DBMDSQ010000000
VERSION     DBMDSQ010000332.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 2194)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2194)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2194
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10943_length_2194_cov_2.223125"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            64..1284
                     /locus_tag="ACZPAX_09630"
     CDS             64..1284
                     /locus_tag="ACZPAX_09630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1114197.1"
                     /translation="MKNHTRLPLLVTAAVFALTACTPVADSTPAAEPTTTPEITAEPT
                     SKPAAAETGPLYTYSHRYAQDVVYDVMPAFNDQLYHIGTNVYKNDLNAGQVSLFYSCD
                     DCCIADPYVTSDAVYLLTYNRDRDNKIIALSPDGTKTHEIPFDPYASTVVLYSDRYFY
                     CMDGHAPYDTASGFRLDLQTGETTPWDLPAETTGAQDATGDFAVTARLISDHPVPFTS
                     DPEISDALLQNSMLEYDLTDATTGKPATKIAEFPYKGADDGSGYVYYTYLGKSGDDFY
                     FTGEHSRSEEEGIKMSVLRVGSDGTQEDLGLMIHVSLRELRQNGEIQWLFAMSSNPGT
                     LTVYDLQGTQIAQVDPPAGVESYYPISMLDDGRILLNIGYDLDHDYMTRYATIDADAF
                     LNGSTEYTEMEFVG"
     gene            complement(1544..>2194)
                     /locus_tag="ACZPAX_09635"
     CDS             complement(1544..>2194)
                     /locus_tag="ACZPAX_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HIR1114198.1"
                     /translation="PETCTECGGRGYVVTQQRTPFGVMQSQQPCPHCGGRGTIIKNPC
                     KTCRGTGKTSARKTLEVKIPAGIDDDQNIALRGQGDAGTNGGPAGDVIVHVTVKPDAV
                     FERDGYDVYVRVPITYSQAVLGAEIEVPTVDGKVAQKIPEGTQSGTKFRLRGQGIQYL
                     NGRGRGDQYVIVDVEIPKKLNRTQREALKAFEDSLKDENYEKRKNFFKNLKDRFMS"
BASE COUNT          449 a          683 c          627 g          435 t
ORIGIN      
        1 acaagtagga cacaactggg gtgtagaatg acgacaaacc acaaatgaaa ggagttatca
       61 cccatgaaaa accacacccg cctgcccctg cttgtcaccg ccgccgtgtt cgccctgacc
      121 gcctgcaccc cggtggcgga ttccacgccc gccgcggaac ccaccaccac gccggaaatc
      181 accgctgaac cgacaagcaa gcccgctgct gctgagaccg gcccactcta tacttacagc
      241 caccgctatg cgcaggatgt tgtctatgat gtcatgcctg cctttaatga ccagttgtac
      301 cacatcggaa cgaacgttta caaaaacgac ctgaacgccg ggcaggtttc gctgttttac
      361 agttgcgacg actgctgcat cgccgacccc tatgtgacga gcgacgcggt gtacctgctc
      421 acctacaaca gagaccgcga caacaaaatc attgccctgt cgccggatgg caccaaaacc
      481 cacgagattc cctttgaccc atacgcctcg accgttgtct tatacagcga ccgttatttt
      541 tattgcatgg acggtcatgc cccctatgat actgcaagcg ggttccgcct tgacttgcag
      601 acaggcgaga caacaccctg ggacttgccc gccgagacaa ctggcgcaca ggacgccacc
      661 ggtgattttg ccgtgaccgc gcggctcatt tcagaccacc ctgttccctt cacgagcgac
      721 ccggaaattt ccgatgccct tttgcagaac tctatgctcg agtacgacct gacggacgcc
      781 acaaccggaa agcccgccac gaaaattgca gagtttccgt ataaaggtgc ggacgacggc
      841 agcggctatg tctattacac ctaccttggg aaatccggag acgatttcta cttcaccggc
      901 gagcactccc ggtcggagga ggagggcatt aaaatgtctg tgctccgtgt gggcagcgac
      961 ggcacgcagg aagatttagg gcttatgatc catgtgtcgc ttcgcgaatt gcgccaaaac
     1021 ggagagattc agtggctttt cgcaatgagc agcaacccgg gcaccctcac agtatacgac
     1081 ctgcaaggca cgcagattgc gcaggtggac ccgcccgccg gggtggagtc ctactatccg
     1141 atttccatgc tggacgatgg acggattttg ttgaacattg gctatgatct cgaccacgac
     1201 tacatgaccc ggtacgccac catcgacgcc gacgcgtttt tgaatggcag caccgagtat
     1261 accgagatgg aatttgtggg gtaaagggca cccccctgcg gcggggtgag ggcaccccgc
     1321 cctacaagtt acggtacggt gggccgtagg gcggcatgcc cccatgccgc cgggcacgtt
     1381 tccgccgccg caatgctccc gggtcgtcgg ggacgccgac ccctgcaacg gtgggcgcag
     1441 ctgcggcggc cagaggactg gccgccctac aacatgccat tcataaaata cagccccgcc
     1501 ccggcaatgt ggaaaacatt gccggggcgg ggctattcaa aatttaagac atgaaccgat
     1561 ccttcaggtt tttgaagaag ttcttgcgct tctcatagtt ctcgtccttc aggctgtcct
     1621 caaaggcctt cagggcctcg cgctgggtgc gattcagctt tttgggaatc tccacatcca
     1681 cgatgacata ctggtcaccg cggccgcggc cgttcagata ttggatgccc tggccgcgca
     1741 ggcggaactt cgtgccgctc tgggtgccct cggggatctt ctgggcgacc ttgccgtcca
     1801 cagtaggcac ttcaatttca gcgcccagga cggcctggga gtaggtaatc ggcacgcgga
     1861 cgtaaacgtc gtagccgtcg cgctcaaaca cggcatcggg cttgaccgtc acatggacga
     1921 tcacgtcacc ggcggggccg ccgttggtgc ctgcgtcgcc ctggccgcgc agcgcgatgt
     1981 tctggtcgtc gtcgataccg gcgggaatct tgacttccag cgtcttgcgg gcgctggtct
     2041 taccggtgcc gcggcaggtc ttgcaggggt tcttgatgat ggtgccgcgg ccgccgcagt
     2101 gcgggcaggg ctgctggctc tgcatcacgc caaagggcgt gcgctgctgg gtcacgacat
     2161 agccgcggcc gccgcattcg gtacaggtct cggg
//
LOCUS       DBMDSQ010000333         2194 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_10946_length_2194_cov_1.565695, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000333 DBMDSQ010000000
VERSION     DBMDSQ010000333.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 2194)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2194)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2194
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10946_length_2194_cov_1.565695"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(12..1118)
                     /locus_tag="ACZPAX_09640"
     CDS             complement(12..1118)
                     /locus_tag="ACZPAX_09640"
                     /EC_number="4.2.1.51"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HIR1114199.1"
                     /translation="MDLLQQARAEIDMVDAEMAALFERRMCAVADVARYKAETGKPVF
                     DAAREAAVLDKNTARIADETLRPYYRAFLNDAMAVSRAYQRAQLGRDTAAYQGVPGAW
                     SHITLRRLFPFARESAFATWGEVFDAVQKGDAQFGVLPFENSSAGDVSTVLDLLYTHP
                     EIIVSRMCDMHIRQDLVAVPGATMETIKTVVSHPQALAQSSVFVQQHGFKTATWGNTA
                     DAARHVAELGDPTVAAIASAETAELYGLQILSPGINAEGDNTTRFIVIERADTAPTMT
                     GEGQRLALLFTARHKPGQLAAALDQIGARGFNMECIKSRPLPHVPFEYYFYVQLVCPA
                     GSTGAECQALLDTLTPVCSTLRLLGAFTLDGTEK"
     gene            complement(1285..2127)
                     /locus_tag="ACZPAX_09645"
     CDS             complement(1285..2127)
                     /locus_tag="ACZPAX_09645"
                     /inference="COORDINATES: protein motif:HMM:NF012905.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HIR1114200.1"
                     /translation="MANALTDFTKKREFLVCMDSDGCVMDTVRIKHSTVMCPELIRVF
                     ALDDYAEFITAAWDEINLHTITRGISRFESVRLVFDRLKNRGIEIPGSEDIAAWVDTA
                     NELSTASLQRELQKTGSLALRKLQEWNNACNRRIQALEPTFEPFPGVEESLRQLHAVA
                     DVAVVSAANESAIASEWKRYGLARHADVIFGQEVGSKANSIATMLACGYESRKVMMVG
                     DAMGDAQAAAANGVAFVPILPGREADSWRRLQEEALPKLLHGTFSPEYQAELLAALRS
                     ALHG"
BASE COUNT          418 a          685 c          653 g          438 t
ORIGIN      
        1 ttcttcatgg cttatttctc cgtaccgtcc agggtgaacg cacccagcag ccgcagggtg
       61 ctgcaaacag gggtcagggt gtccagcagg gcctggcact ctgcgccggt actgcccgcc
      121 gggcagacca gctgcacata gaaataatat tcaaacggaa catggggcag ggggcggctc
      181 ttgatgcact ccatgttaaa gccccgtgcg ccgatctgat ccagcgcggc ggccagctgg
      241 ccgggcttgt ggcgtgctgt gaacagcagc gccagccgct ggccctcgcc ggtcatggtg
      301 ggggcggtgt cggcgcgttc aatgacgatg aaccgcgtcg tgttgtcgcc ttcggcgttg
      361 atgccggggg agagaatttg cagcccgtac agttccgccg tctcggcgct ggcaatggcc
      421 gcaacggtgg ggtcgcccag ctcagccaca tggcgggcgg catcggccgt gttgccccag
      481 gtggccgttt taaagccgtg ctgctgcaca aaaacgctgc tttgggccag ggcctgcggg
      541 tggctgacaa cggtcttgat cgtctccatc gtcgcgccgg gcacagctac caggtcttgc
      601 cggatgtgca tatcgcacat tctggaaacg atgatttcgg ggtgggtgta cagcaggtcc
      661 agcacggtgg aaacatcgcc cgcgctggaa ttttcaaacg gcagaacgcc gaattgcgcg
      721 tcgcccttct gcacagcgtc aaagacctcg ccccaggtgg caaaggcgga ttcccgcgca
      781 aaggggaaga gccgccgcag cgtgatatgg ctccacgcgc cgggcacacc ctgataggcg
      841 gcggtgtccc ggcccagctg tgcccgctga tacgcacggg aaaccgccat ggcatcgttt
      901 aaaaatgcgc ggtagtaggg gcgcagcgtc tcatcggcga tgcgggccgt gtttttgtcc
      961 agcacagcag cctcccgcgc ggcgtcaaag accggcttgc cggtctcggc cttatagcgt
     1021 gccacatcgg ccaccgcgca catccgccgc tcaaacaggg cggccatctc cgcatcgacc
     1081 atatcaattt ctgcccgggc ctgctgtaat aagtccatac atgaggctcc ttttcccaga
     1141 tgaattttgc atttccgcag cgccccgccc ctacggtaca tcagcatgac acgcgctgtt
     1201 tatttatgca gataattata gtcagtatac cataaataaa gcgccgtcac aagagtgacg
     1261 gcgcgaattt tggcgtattt tagcttaccc gtgcagggcg ctgcgcaacg cggccagcag
     1321 ttcagcctga tattccgggc tgaaggtgcc gtgcagcagc ttcggcaggg cttcctcctg
     1381 caagcgccgc cagctgtcgg cctcgcggcc cggcagaatc ggcacaaagg ccacgccgtt
     1441 ggcggcggcg gcctgggcgt cgcccatagc gtcacccacc atcatgacct tgcggctctc
     1501 atacccgcaa gccagcattg tggcgatgga gtttgccttg ctgcccactt cctggccgaa
     1561 gatgacatcc gcgtggcggg ccaggccgta acgcttccac tcactggcaa tggcgctctc
     1621 gttcgcggcg ctgacaacgg ccacatcggc gaccgcgtgc agctggcgca ggctttcttc
     1681 tacaccgggg aacggctcaa acgtcggctc caaggcctgg atgcggcggt tgcaggcatt
     1741 gttccactcc tgcaatttgc gcagggccag gctgcctgtc ttttgcagct cccgctgcag
     1801 gctggccgta gaaagctcgt tggcggtatc gacccaggcg gcaatatcct cgctgccggg
     1861 aatctcgatg ccgcggtttt tcagccggtc aaacaccagc cgcacgctct caaaccggga
     1921 gatgccccgg gtgatggtgt gcaggttgat ctcgtcccag gctgccgtga taaactccgc
     1981 gtaatcatcc agcgcaaaca cgcggatgag ctcggggcac atgacggtcg aatgcttgat
     2041 gcgcactgtg tccatcacgc agccgtcgga gtccatacag accaaaaatt cacgtttttt
     2101 ggtaaaatcc gtcagtgcgt ttgccataaa accgtccccc tctatgtact gttccctata
     2161 aaaacgctat gaatctatca taacaaaaat cccc
//
LOCUS       DBMDSQ010000334         2176 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_11077_length_2176_cov_1.585542, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000334 DBMDSQ010000000
VERSION     DBMDSQ010000334.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 2176)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2176)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2176
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11077_length_2176_cov_1.585542"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..549
                     /locus_tag="ACZPAX_09650"
     CDS             <1..549
                     /locus_tag="ACZPAX_09650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HIR1114201.1"
                     /translation="DAVQAALDAVDRTKSKAQQAEVAVMAKNIEDAIAALTYKPADYT
                     AVDAAIAKAGALNRDDYTDFSAVDAAINAVDRGKNITQQADVDAMAKAIETALAALVV
                     KPTATPAPTEAPTPVPAAPAATAQPTAAPTATPAPVVTATPQPPVTIPQTGDSMNVTL
                     LFALALCSGAALGLAAHKKNRS"
     gene            697..1215
                     /locus_tag="ACZPAX_09655"
     CDS             697..1215
                     /locus_tag="ACZPAX_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004602741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HIR1114202.1"
                     /translation="MSKILVAYFSRAGENYFGGAIKSVAVGNTEICADLIKQAFPDAD
                     LFKIEMAAPYADNYRKCVSQVTADMMLHKRPALTALPESIAAYDAIVLGYPNYCGTIP
                     MAVATFLEAFDFTGKKLLPYCTNEGSGMGRSEKDLRKLCPTAVIAPGLAITGSAAAQS
                     QAALVGWMQENL"
     gene            complement(1286..1888)
                     /locus_tag="ACZPAX_09660"
     CDS             complement(1286..1888)
                     /locus_tag="ACZPAX_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HIR1114203.1"
                     /translation="MQDYHAEFVEIYNKNITRPGADRLLNWLETTDFFTAPASTRFHG
                     ACECGLVMHSINVYNAMMQHFYTEGENAESYAICGLLHDLCKANFYKVSTRNVKNDAT
                     GQWEKVPFYQVADQLPYGHGEKSVYLIEHFMRLKTAEAIAIRWHMGGFDDAARGGCFA
                     VSEAYNTYPLAVKLHIADLTATYLMEQGTSEVENGHPKNK"
     gene            complement(1896..2117)
                     /locus_tag="ACZPAX_09665"
     CDS             complement(1896..2117)
                     /locus_tag="ACZPAX_09665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015536721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HIR1114204.1"
                     /translation="MAEFTYEVTERIAVLSTNARGWERQLNMVSWNGNPPKYDIRDWS
                     PDGSRMAKGISLTEDELKTLKGILDDMEL"
BASE COUNT          429 a          688 c          617 g          442 t
ORIGIN      
        1 gacgccgtac aggccgcgct tgacgccgtc gaccgcacca agagcaaggc acagcaagcc
       61 gaggtcgctg tcatggcgaa gaacattgag gatgccatcg ccgcattgac ctacaagccc
      121 gccgactaca ccgcggtcga tgccgcgatt gccaaggcgg gcgcattgaa ccgcgacgac
      181 tacacggatt tctccgctgt ggatgccgcc atcaacgccg tggaccgcgg caagaacatc
      241 acccagcagg ccgatgtgga tgccatggca aaggccatcg agaccgccct cgctgcgctg
      301 gtcgtcaagc ccacggccac cccggccccc acagaagccc ccacgcccgt acctgctgcc
      361 ccggcggcca cggcacagcc caccgccgca cctacggcca ccccggcccc ggtcgttacg
      421 gccacgccgc agccccctgt gaccattccc cagaccggcg acagcatgaa cgtcacgctg
      481 ctgtttgcgc tggccctgtg cagcggtgca gcgctcggcc tggccgccca caagaaaaac
      541 cgttcttgat accgcataaa aacagcgccg cgcaggcttt ttcgggcctg cgcggcattt
      601 tgtctgccgg ggcgtaaaat tttgcattta ccccttgcac ttaaagttgc ctttcatggt
      661 atactgccat atagaatcca acggaggtgc tgttttatgt ctaaaattct ggttgcttat
      721 ttctcccgcg cgggcgaaaa ttactttggc ggcgcaatca aaagcgtggc cgtcggcaat
      781 accgagatct gcgccgacct tatcaagcag gctttccccg acgccgacct gttcaaaatt
      841 gaaatggcag cgccttatgc ggacaactac cgcaaatgcg tcagccaggt caccgcagac
      901 atgatgctgc acaagcgccc ggcgctgacc gccctgcccg agagcattgc ggcctacgac
      961 gccattgtgc tgggctaccc caactactgc ggcaccatcc ccatggcggt ggcgaccttt
     1021 ttggaggcgt tcgactttac cggcaaaaag ctgctgccct actgcaccaa cgagggcagc
     1081 ggcatgggcc gcagcgagaa agacctgcgc aagctctgcc ccacagcggt aattgccccg
     1141 ggtcttgcca tcaccggcag cgcggcggca cagtcccaag ccgcgctggt cgggtggatg
     1201 caggaaaatt tgtaaccccc cctgcggggc gtcggggacg ccgcaaaaaa accagggcac
     1261 agactgcatc gtctgtgccc tggttttatt tgtttttcgg gtgaccgttt tctacttcgc
     1321 tggtgccctg ctccatcagg taggtggcgg tcaggtccgc aatgtgcagc ttgaccgcca
     1381 gcgggtaggt gttgtaggct tcgctgacag caaagcagcc gccgcgcgcg gcatcgtcaa
     1441 agccgcccat gtgccagcgg atggcaatag cctcggcggt tttcagccgc ataaaatgct
     1501 cgatgaggta gacactcttc tcgccgtggc cgtagggcag ttgatccgcc acctggtaaa
     1561 acgggacctt ctcccactgg ccggtggcgt cgtttttgac attgcgggtg ctgaccttat
     1621 agaagtttgc cttgcacagg tcgtgcagca ggccgcagat ggcataactc tcggcgttct
     1681 cgccctcagt gtagaagtgc tgcatcattg cgttgtacac gttgatgctg tgcatgacca
     1741 ggccgcactc gcacgcgccg tggaaccggg tggaggcagg cgcggtgaaa aagtcggtgg
     1801 tttccagcca gttcagcagc cggtccgcac cggggcgggt gatgtttttg ttgtagatct
     1861 ccacaaattc agcgtggtag tcctgcatac gaacctcaca gttccatgtc gtccagaatg
     1921 cccttcagcg ttttcagctc atcctcggtc aggctgattc cctttgccat gcggctgccg
     1981 tccggggacc agtcgcggat atcgtacttc ggcgggttgc cgttccagct gaccatattc
     2041 agctgacgct cccagccgcg ggcgttggtg gaaagaactg cgatgcgttc ggtaacttca
     2101 taggtaaatt ctgccatgat acagactcct tttataaatg atgttgcaag ctccatcata
     2161 gacgattctg cccaaa
//
LOCUS       DBMDSQ010000335         2167 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_11143_length_2167_cov_1.377541, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000335 DBMDSQ010000000
VERSION     DBMDSQ010000335.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2167)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11143_length_2167_cov_1.377541"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1370
                     /locus_tag="ACZPAX_09670"
     CDS             <1..1370
                     /locus_tag="ACZPAX_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014117891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase-related protein"
                     /protein_id="HIR1114205.1"
                     /translation="RNIVVQGGTFYNEAVLRAFEKEMGVNVIRPDIAGLMGAYGAALY
                     GKSRAGENAHSTVLTEAELEAFSQKVNTVQCNGCGNHCQLTVNVFADGKRFISGNRCD
                     KPVTGKANNEDLDLYAYKLKLLDDYRKAAAPASPRAKIGIPLCLNMYELLPFWHTLFT
                     KLGFEVVVSPFSNRKLYQSGQATIPSDTACFPAKLTHGHIRWLCEQGVDAIFYPCMSY
                     NLDEHLGDNHYNCPVVAYYPEVLEGNCPELNATKFIYDYFNLERRKDFYKKFQQALDK
                     YFPGLDKKAVHEAIDAAYDEYARHMQQLRDKGTEIIAAARKEGKHIVVLAGRPYHVDP
                     EVNHGINQLIIRQGAAVVSEDSVSWHEQKFQTAVLNQWTYHSRLYAAAKYCTEQPDMD
                     LVQLVSFGCGLDAVTTDETREILQAGGKLYTQLKIDEITNLGAVNIRLRSLFAALEER
                     KEDHK"
     gene            1381..>2167
                     /locus_tag="ACZPAX_09675"
     CDS             1381..>2167
                     /locus_tag="ACZPAX_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005925121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyglutaryl-CoA dehydratase"
                     /protein_id="HIR1114206.1"
                     /translation="MEYMTPNFTKDMVKTHKILIPNMAVTQFRLMQAALASEGYQTEV
                     LGNCGSEVAQLGLKYVHNDTCYPALLVIGQFLDALNSGKYDLDHTALLITQTGGGCRA
                     SNYIKLLRKALVKAGYGNIPVASLNFSGLEKGSGLPLTVPLLRKVIASIFYGDMLVAL
                     RSQTHAYEDHKGDADAMTEKWIATIQSWIRGDRNYSAHDMKRRFYDIAKDYATIPITR
                     VPKVKVGVVGEIYVKYSPLGNNDLEKFLESQDCWSTRTKCSTSR"
BASE COUNT          490 a          692 c          611 g          374 t
ORIGIN      
        1 gccgcaacat cgtcgtgcag ggtggtactt tctataatga ggccgtcctc cgcgcctttg
       61 aaaaagagat gggcgtcaac gtcatccgcc ccgacattgc gggcctgatg ggcgcttacg
      121 gcgcagcctt gtacggcaag agccgcgcgg gcgagaacgc ccacagcacc gtcctgaccg
      181 aggccgagct ggaagcgttc agccagaagg tcaacaccgt gcagtgcaac ggctgcggca
      241 accactgcca gctgaccgtc aacgtttttg ccgatggcaa gcggtttatc tccggcaacc
      301 ggtgcgataa acccgtgacc ggcaaggcca acaatgagga tctggacctc tacgcctaca
      361 agctgaagct gctggacgac taccgcaagg cggccgcccc ggccagcccc cgcgccaaaa
      421 tcggcatccc gctctgcctg aatatgtacg agctgctgcc gttctggcac acactgttca
      481 ccaaattggg ctttgaggtc gtcgtcagtc ccttctccaa ccgcaagctg taccagagcg
      541 gccaggcgac catacccagc gacacggcct gcttcccggc caagctgacc cacggccaca
      601 tccgctggct gtgtgagcag ggcgtggatg cgatcttcta cccctgcatg agctataacc
      661 tggacgagca cctgggtgac aaccattata actgcccggt cgtcgcctat taccccgagg
      721 ttttggaggg caactgcccc gagctgaacg cgacgaaatt catctacgac tacttcaacc
      781 tcgagcgccg caaggatttc tacaagaaat tccagcaggc gctggacaaa tatttcccgg
      841 ggctggacaa aaaggccgtg catgaggcca tcgatgcggc ttacgacgag tacgcccgcc
      901 atatgcagca gctgcgcgac aagggtaccg agatcatcgc cgctgcccgc aaggaaggca
      961 agcatatcgt cgtgctggcg ggccgcccct accacgttga cccagaggtc aaccacggca
     1021 tcaaccagct tattatccgc cagggcgcgg ccgtcgtcag cgaggattcc gtcagctggc
     1081 acgagcagaa gttccaaacc gccgtgctga accagtggac ctaccacagc cgcctgtatg
     1141 ccgccgccaa gtactgcacc gaacagcccg acatggatct ggtgcagctg gtgtccttcg
     1201 gctgcggcct ggacgccgtg accaccgacg agacccgcga gattttgcag gcgggcggca
     1261 agctgtacac ccagctgaaa atcgacgaga tcacgaacct gggcgcggtg aacatccgcc
     1321 tgcgcagcct gtttgcagca ctggaagaac ggaaagaaga ccataagtaa gaggcaaccc
     1381 atggaataca tgactcccaa ttttaccaag gatatggtaa agacccataa aatcctgatt
     1441 cccaacatgg cggtcaccca gttccggctc atgcaggcgg cgctggccag cgagggctac
     1501 cagaccgagg tgctgggcaa ctgcggcagc gaggtcgccc agctgggcct gaaatacgtc
     1561 cataacgaca cctgttaccc ggcgcttctg gtcatcggtc agttcttgga tgcgctgaac
     1621 agcggcaagt acgacctgga ccacacggcg ctgctcatca cccagaccgg cggcggctgc
     1681 cgcgcgtcca actacatcaa gctgctgcgc aaggccctcg tcaaggcggg ctacggcaat
     1741 atcccggtcg cgtcgctgaa cttctccggt ctggaaaagg gcagcggcct gccgctgact
     1801 gtgccgctgc tgcgcaaagt cattgcatcg attttctacg gtgatatgct ggtggccctg
     1861 cgcagccaga cccacgccta tgaggatcac aagggcgacg ccgacgccat gaccgaaaag
     1921 tggatcgcca ccatccagag ctggattcgc ggcgacagga actactccgc ccacgatatg
     1981 aaacgccgct tctacgacat cgcgaaggac tacgccacca tccccatcac ccgcgtaccg
     2041 aaggtcaagg tcggcgttgt cggcgaaatt tacgtcaagt attcgccgct gggcaataac
     2101 gatttggaaa aatttcttga gagccaggac tgctggtcaa cccgcacgaa atgctcgaca
     2161 tcacggc
//
LOCUS       DBMDSQ010000336         2164 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_11161_length_2164_cov_1.661173, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000336 DBMDSQ010000000
VERSION     DBMDSQ010000336.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 2164)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2164)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2164
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11161_length_2164_cov_1.661173"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="ACZPAX_09680"
     CDS             <1..243
                     /locus_tag="ACZPAX_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005925913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HIR1114207.1"
                     /translation="LLHQTAKGHQVLCITHTPQVAAFADNQLLIQKNVRNDRTFTEIH
                     TLDMDGRVEVLARMISGDKVSELSLASARELIEKSK"
     gene            666..1886
                     /gene="tyrS"
                     /locus_tag="ACZPAX_09685"
     CDS             666..1886
                     /gene="tyrS"
                     /locus_tag="ACZPAX_09685"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005925907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HIR1114208.1"
                     /translation="MTIYDELKARGLIAQVTDEDEIKELINNGKATFYIGFDCTADSL
                     TAGHFMALTLMKRLQQAGNRPIALIGGGTTMIGDPSGRTDMRKMLTKEDIDHNAECFK
                     RQMEKFIEFGEGKAMMLNNADWLLNLNYIELLREVGPCFSVNNMLRAECYKQRMEKGL
                     SFLEFNYMIMQSYDFYHLFQNYGCNMEFGGDDQWSNMLGGTELIRRKLGKDAYAMTIT
                     LLTDSQGKKMGKTAGNAVWLDPNKTSPFEFYQYWRNVGDADVLKCIRMLTFLPLEQID
                     EMDHWEGEQLNKAKEILAYELTKMVHGEAEAEKAQATARGLFSGAADHEHMPSTALDA
                     ALVKDGAVGLLAAMVAAGLCGSNREARQLVQQGGVLVDGEKVTDPKAVLTVDALNKGV
                     VIKKGKKVYHKVTL"
BASE COUNT          521 a          578 c          622 g          443 t
ORIGIN      
        1 cttttacacc agacagccaa ggggcatcag gtgctgtgca tcacccacac gccgcaggtg
       61 gctgcgtttg cggacaacca gctgctcatc cagaaaaacg tccgcaacga ccgcacgttt
      121 acggaaattc atactctcga tatggatggt cgcgtggaag tgctggcgcg gatgatttcc
      181 ggcgacaagg tcagcgaatt gagccttgcc agtgcgcggg agcttatcga aaagtcgaaa
      241 tgacggcggc gggcttgacg cgagcatcgg cccctacaat gcgtagaggc acattccata
      301 tgtgcccgcc tcttgacaat ccgtttagaa tatgatacag taaaccgtgt taaatgcagt
      361 gatgggaacc agtacccatc tgccacgctt cagagaagcc ccggtcggtg caaggggtca
      421 gcaggtgcag atggcgaata cagcccggag cagcaggctg aacgcttagg ctagtaggct
      481 gtgccgtgtg cgcacgttac agcgtttggg tgtacgcagt cctttgtctg ccgcacccgc
      541 gaaggcccgc cgccgtgagg cacgggcaaa cagaggtggt accgcgaact ttcgccctct
      601 gccaaattca catttggcag gggcttttat ttttgccctt gccgcaacaa acggaggtaa
      661 aacccatgac gatttacgac gaactgaaag cccgcggcct gattgcacag gtcacggacg
      721 aggatgagat caaggagctg atcaacaacg gcaaggcaac cttctacatc ggctttgact
      781 gcacggccga cagcctgacc gccggtcact ttatggccct gacccttatg aagcgcttgc
      841 aacaggccgg caaccgcccg attgccctga tcggcggcgg cacaacgatg attggcgatc
      901 cctccggccg caccgacatg cgcaagatgc tgaccaagga ggacatcgac cacaacgccg
      961 agtgctttaa gcgtcagatg gaaaaattca ttgagttcgg cgaaggcaag gccatgatgc
     1021 tgaacaacgc cgactggctg ctgaacctga actatatcga gctgctgcgc gaggtcggtc
     1081 cctgcttcag cgtaaataat atgctgcgcg ccgagtgcta caagcagcgc atggagaagg
     1141 gcctgtcctt cctcgagttc aactacatga tcatgcagag ctacgatttc taccatctgt
     1201 tccagaatta cggctgcaac atggaatttg gcggcgatga ccagtggagc aacatgctgg
     1261 gcggcaccga gctgatccgc cgcaagctgg gtaaggatgc ctacgccatg accatcacgc
     1321 tgctgaccga ctctcagggc aagaagatgg gcaagaccgc aggcaacgct gtctggcttg
     1381 accccaacaa gacgagcccg ttcgaattct accagtactg gcgcaacgtc ggcgatgcgg
     1441 acgttctcaa gtgcatccgt atgctgacct tcctgccgct ggagcagatc gacgagatgg
     1501 accactggga gggcgagcag ctgaacaagg ccaaggagat tctggcctac gagctgacca
     1561 agatggtcca cggcgaggca gaagccgaga aggcgcaggc taccgcccgc ggcctcttca
     1621 gcggtgccgc cgatcatgag catatgccca gcacggcgct ggatgcagcg cttgtcaagg
     1681 acggcgctgt cggcctgctg gctgccatgg tagctgccgg tctgtgcggc tccaaccgcg
     1741 aggcccgcca gctggtccag cagggcggcg tccttgttga cggtgagaag gtcaccgacc
     1801 cgaaggcagt tctgaccgtt gacgcgctga ataagggcgt tgtcatcaag aagggcaaga
     1861 aggtttacca caaggtcacg ctgtaaagca aagttgcttc acagtcaatt tcatcacaca
     1921 ctataagtaa acaagccccc tgtccgcacg gcaaatgttg ttggacaggg ggcttttggt
     1981 gtagtataat ggaacaaatc ggaagttgtg gaggtagaaa aacatgtata gaatatatag
     2041 tgcacttatc gaaattgttg cggcagctgt ttttataatt cctatttggt gcatatataa
     2101 caagttatgt tttcatagct ggaaacgaac tattatttat atggtattgg ggttttattt
     2161 tact
//
LOCUS       DBMDSQ010000337         2158 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_11199_length_2158_cov_1.680603, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000337 DBMDSQ010000000
VERSION     DBMDSQ010000337.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 2158)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2158)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2158
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11199_length_2158_cov_1.680603"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..608)
                     /locus_tag="ACZPAX_09690"
     CDS             complement(<1..608)
                     /locus_tag="ACZPAX_09690"
                     /inference="COORDINATES: protein motif:HMM:NF012742.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 periplasmic-binding domain-containing
                     protein"
                     /protein_id="HIR1114209.1"
                     /translation="MGEETRARVLAYAGLTGREAAPPHPVTQGATHNLSLIIPKHFIR
                     LDLPFLRKCMGGICAMAAQRGYDLLLCYVSETDTDQLERQLASHKVDGVILSRTMSDD
                     PCIDLLNQYGVPFVAIGRIDDPEIPQADNDQLGAACEMTRLLLQLGTRRIAYLGGSGT
                     YTVNADRLRGYLRGLAEFGVQADQTLIHTGLESDEQRTDALEA"
     gene            complement(624..893)
                     /locus_tag="ACZPAX_09695"
                     /pseudo
     CDS             complement(624..893)
                     /locus_tag="ACZPAX_09695"
                     /inference="COORDINATES: protein motif:HMM:NF012576.6"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            1151..1615
                     /locus_tag="ACZPAX_09700"
     CDS             1151..1615
                     /locus_tag="ACZPAX_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015564847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HIR1114210.1"
                     /translation="MMVPYMFNDGLMDDLFSDAWAHDFDRAMRAAEPRSMFGKRAANV
                     MKTDVRENKDSYDVFVDLPGFKKEDVKLDLDNGYLTISAQRNEELDEKDNEGRYIRQE
                     RATGSCARSFYVGKELEPKDISAKFENGILSLHLPKADAKKLEPKQTLIEIE"
     gene            1784..>2158
                     /locus_tag="ACZPAX_09705"
     CDS             1784..>2158
                     /locus_tag="ACZPAX_09705"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007048620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HIR1114211.1"
                     /translation="MPYSDEHLRYLRLLSQKYKTVAAAASEIIRIEALLRLPKGTEHF
                     MSDLHGEHEAFVHILNSASGVIREKIDTVLGPGVPADERADLATLVYYPNQKLPELKA
                     RQKDLHAWYRLTLLRLIDLCRFV"
BASE COUNT          506 a          584 c          597 g          471 t
ORIGIN      
        1 gcttccagtg cgtcggtgcg ctgttcgtcg ctctccaatc ctgtgtggat cagcgtttgg
       61 tcggcctgca caccgaactc ggccagacct cgcagatagc cacgcagccg gtcggcgttg
      121 acggtataag tgccgctgcc gcccaggtag gcgatgcgcc gggtgcccaa ctgcagcagc
      181 aagcgggtca tttcacacgc cgcacccagc tggtcgttgt cggcctgggg aatctccggg
      241 tcgtcgatgc ggccgatggc cacaaaaggc acaccgtact gattcaacaa gtcgatgcag
      301 gggtcatccg acatggtgcg ggaaaggatg acaccatcaa ctttatggct tgccagctgg
      361 cgttccagtt ggtcggtgtc ggtctcgctg acatagcaga gcagcaggtc atacccgcgc
      421 tgcgcagcca tggcgcagat gccgcccata catttgcgca ggaagggcaa atccaggcgg
      481 atgaagtgtt tcgggatgat gagtgacaga ttatgtgttg cgccctgtgt caccggatgg
      541 ggcggcgcgg cctcgcgccc ggtcagcccg gcgtaggcaa gcacccgggc gcgggtctcc
      601 tcccccacgc gtccttttcc ggaaagcgca cgtgataccg tggttttcga gattccgagc
      661 gcgtctgcga tctctgcaat tttcatgggt gtagtattat tcgccatggt gcgcacgttc
      721 cctcccttgc tgaaaatgtt gcgcatgata gttgcgcaca gttcaaggat accgttgcat
      781 tcaaaaagtg tcaatccgca aaccgcacaa aaaaacaggc gaaaatttgg caagggtggc
      841 gggcatgttc ttgttttata tggtatgtac acacttgtgc aggggctgcg catattgatc
      901 gctgcacaaa atcacgtatt atcgctgcat ttcaaaactt attgcatatt ttatgaacaa
      961 tcattacaca acttgtaaac attagcactc ttctattgac agtgctaatt tgctgtgcta
     1021 ttatagaggc gttccaaggg gaacaggggc gaaagaacca gaaccccaaa ggaacaacaa
     1081 agcttaattc aaaagtcaac tgtacgcaca gtctacgttc agccgatgaa aattggagga
     1141 atgacttatg atgatggttc cgtatatgtt caatgatggt ttgatggatg atttgttcag
     1201 cgatgcctgg gcgcatgact ttgaccgtgc aatgcgcgct gccgaacccc gcagtatgtt
     1261 tggcaaacgt gccgccaatg tgatgaagac cgacgtccgc gagaacaagg acagttacga
     1321 tgtgtttgtc gacctgcccg gctttaagaa ggaggatgtc aagctcgacc ttgacaacgg
     1381 ctatctgacc atcagcgccc agcgcaatga ggaactggac gagaaggaca acgagggccg
     1441 ctacattcgc caggagcgtg ccaccggcag ttgtgcccgc agcttctacg tcggcaagga
     1501 gctggaaccc aaggatatca gcgccaaatt tgagaacggc atcctgagcc tgcacctgcc
     1561 caaggccgac gccaagaaat tggagccgaa gcagacgtta attgagatcg agtaatcgct
     1621 catcttccgg aacgccgcag cggcggatgt gagcatcgac ccctgcaagg catactcttc
     1681 cgggccgcaa ccctaaattc ttagggctgc ggcccttttg ttttgcccaa aagtgtgtta
     1741 taattccata tagatgaaat aaaagtacag ggaggtcctg cccgtgccat attccgacga
     1801 gcatctgcgc tatctgcgcc tgctttccca gaaatacaag accgtggccg ccgcggccag
     1861 cgagatcatc cgcattgagg cactgctgcg tctgcccaag ggcaccgagc attttatgag
     1921 cgacctgcac ggcgagcatg aagcgtttgt acatatttta aacagcgcgt caggcgtcat
     1981 ccgggaaaag atcgacacgg tgttaggccc cggcgtcccc gccgacgaac gcgccgattt
     2041 ggcaacacta gtctattatc ccaatcaaaa attgcctgaa ctgaaagccc gccaaaaaga
     2101 cctgcacgcc tggtatcggc tgacactgct gcggctcatc gatctgtgcc ggttcgtg
//
LOCUS       DBMDSQ010000338         2150 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_11258_length_2150_cov_1.684724, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000338 DBMDSQ010000000
VERSION     DBMDSQ010000338.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 2150)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2150)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2150
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11258_length_2150_cov_1.684724"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..90)
                     /locus_tag="ACZPAX_09710"
     CDS             complement(<1..90)
                     /locus_tag="ACZPAX_09710"
                     /inference="COORDINATES: protein motif:HMM:NF018648.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HIR1114212.1"
                     /translation="MGVFFGLLVGKPLGIFLASWLAVKTKLAEM"
     gene            89..673
                     /locus_tag="ACZPAX_09715"
     CDS             89..673
                     /locus_tag="ACZPAX_09715"
                     /inference="COORDINATES: protein motif:HMM:NF020260.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair MutL family protein"
                     /protein_id="HIR1114213.1"
                     /translation="MPTASEPLRLVGEVFKTYIITERGGELCLIDKHAAHERILFEQL
                     AKDYGNVPSQMLLVPVQVNLTAAEKQAVLQNIDLLTDAGIEAEDFGGSTVVVRAVPAD
                     VPVDDVEDMIVEVAAKLIDGGRDALREKTEWVLHSIACRAAIKAGDHTNAAEMLALAQ
                     HIMDGTVPPFCPHGRPCVLKITRKELEKQFGRLV"
     gene            657..1598
                     /gene="miaA"
                     /locus_tag="ACZPAX_09720"
     CDS             657..1598
                     /gene="miaA"
                     /locus_tag="ACZPAX_09720"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HIR1114214.1"
                     /translation="MADLSKPRVVAVGGPTASGKTTLSVALAKAFDGEIINADSMQIY
                     KNLDVGTAKPSAEERQGIPHYLLDFLGPEMPYSVADFTAAADPLIRKLTARGKLPVVV
                     GGTGLYITSLLNGMNFAPEKIDPAIRARLQARADAVGGAALYAQLQSIDPDYAAQVHP
                     NNLPRVIRALELYEATGRKMSAERISARAAEPPYRSLCLCLTCRDRAALYDRIGRRVD
                     LMVKNGVLDEAKQVYDHRESYRMAAQAIGYKEFFPYFEGTAPLEECTEKLKQATRNYA
                     KRQLTWFRRQNDAVWLYIDEENVIDRACSLVREFLQN"
     gene            1617..>2150
                     /locus_tag="ACZPAX_09725"
     CDS             1617..>2150
                     /locus_tag="ACZPAX_09725"
                     /inference="COORDINATES: protein motif:HMM:NF024112.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HIR1114215.1"
                     /translation="MEKQPNRTAAKRIFHVVGLLALALVSVYIIVQCFVIFHRSYKTE
                     TAIKYTLAESITLDGVVAFDSVDVAGSGDLGYLVQDGERVTNGTIVAEVYTDDSQGLQ
                     RERLDRLERTITLLTKSQNSTGSDLSVLTNQTKQALYNLLDKINTAQYSGITDAEDTF
                     LLAQNRLQVSTGQTAGFA"
BASE COUNT          467 a          640 c          647 g          396 t
ORIGIN      
        1 catttcggcc agtttcgtct tcacggcaag ccaactggcc aggaaaatac cgagcggctt
       61 gcctacgagc agtccgaaga agacacccat gccgaccgca agcgaaccgc tgcgcctggt
      121 gggcgaggtg ttcaagactt acattattac cgagcgcggc ggtgagttgt gcctcatcga
      181 caagcacgct gcccacgagc gcattttgtt tgagcagctg gcgaaggatt acggcaacgt
      241 gccgtcccag atgctgttgg tgccggtgca ggtcaatctg accgccgccg aaaagcaggc
      301 ggttttgcag aatatcgacc tgttgaccga cgcgggcatc gaggcggagg atttcggcgg
      361 ttccaccgtc gttgtccgcg ctgtgcccgc cgatgtgccg gtggacgacg tggaggatat
      421 gatcgtggaa gtcgcggcga agctgatcga cggcggccgc gacgctttgc gggaaaagac
      481 cgagtgggtg ttgcattcca tcgcctgccg cgccgccatc aaggcgggcg accacacgaa
      541 cgctgccgaa atgctggcgc tggcccagca catcatggat ggcacggttc cgccgttctg
      601 cccccatggg cggccctgcg tgctgaaaat cacccggaag gagctggaaa agcagtttgg
      661 ccgacttgtc taaaccccgc gttgtcgcgg tcggcggccc cacggccagc ggcaagacca
      721 cgctttccgt cgcgctggca aaggcgttcg acggtgagat catcaacgcg gactctatgc
      781 agatttataa aaatcttgac gtaggaacgg caaaacctag cgcagaagag cggcagggga
      841 taccacacta cctgctggac tttttagggc cggaaatgcc gtacagcgtg gcagatttca
      901 ccgccgccgc cgatccgttg attcggaaac tcacggcacg cggcaagttg cccgtcgttg
      961 tggggggcac aggactttat atcaccagcc ttttgaacgg catgaacttt gccccggaaa
     1021 aaatcgaccc ggctatccgc gcccgcctgc aagcccgggc cgacgccgtg ggcggcgcag
     1081 cgctgtacgc ccaactgcaa agcattgatc ccgattacgc cgcgcaggtc cacccaaaca
     1141 acctgccccg cgtcatccgt gcgctggaat tgtacgaagc cacaggccgc aaaatgagtg
     1201 cggagcgcat cagcgcccgg gctgccgagc caccgtaccg ttcgctctgc ctttgcctga
     1261 cctgccgcga ccgcgcggcg ctctatgacc gcatcggccg cagggtggac ttaatggtga
     1321 aaaacggcgt gttggacgag gcaaaacagg tctatgacca tcgcgagagc taccgcatgg
     1381 cggcccaggc catcggctac aaggagttct ttccgtattt tgaaggcacg gcgcccttgg
     1441 aggaatgcac cgaaaaactc aagcaggcca cccgcaacta cgccaagcgg cagctgacct
     1501 ggttccgccg ccagaacgac gcggtctggc tttatattga tgaagaaaac gtcatcgacc
     1561 gcgcctgttc gctggtgcgt gaatttttgc aaaactgaca gaaaggggag tgtgcagtgg
     1621 aaaaacagcc gaaccgcaca gcggcaaaga gaatttttca tgtggtcggg ctgctggcac
     1681 tggcactggt ttctgtctac attattgtac agtgtttcgt tatttttcac cgctcctaca
     1741 aaacagagac tgccatcaag tacacgttgg ccgagagcat taccctcgac ggcgttgtgg
     1801 cgtttgactc ggtggatgtg gcaggcagcg gcgaccttgg ctaccttgtc caggacggtg
     1861 agcgcgtcac aaacggaacc atcgtggccg aagtgtacac cgatgattcc cagggattgc
     1921 agcgcgagcg gcttgaccgg ctcgagcgca ccatcacgct gctgacaaag tcccagaact
     1981 ccaccggcag cgacctgtcg gtgctgacga accagacaaa acaggcgctg tacaacctgc
     2041 tggataagat caacacggct cagtacagcg gcattaccga cgccgaggat accttcctgc
     2101 tggcgcagaa ccgtctgcag gtcagcaccg gccagacggc gggctttgca
//
LOCUS       DBMDSQ010000339         2147 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_11279_length_2147_cov_1.921310, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000339 DBMDSQ010000000
VERSION     DBMDSQ010000339.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 2147)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2147)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2147
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11279_length_2147_cov_1.921310"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            44..166
                     /locus_tag="ACZPAX_09730"
     CDS             44..166
                     /locus_tag="ACZPAX_09730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1114216.1"
                     /translation="MGERFGKEAVNGITVYPLQVSAVETAALRWMRTMAATEYE"
     gene            224..1234
                     /gene="trpS"
                     /locus_tag="ACZPAX_09735"
     CDS             224..1234
                     /gene="trpS"
                     /locus_tag="ACZPAX_09735"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HIR1114217.1"
                     /translation="MAEEKRKRILSGIQPTGTPTLGNYIGAVRNWALLQSDYDCLYMV
                     ADLHSLTVRQVPADLRRRTRELAALLLAVGIDPKEHVLFVQSHVPAHTELSWVLACNT
                     QFGELSRMTQFKDKSAKHPDNVNGGLFTYPVLMAADILIYNADLVPVGQDQTQHLEIA
                     RDIAGRFNGIYGDTFTLPEGYVPAAGAKIMSLAEPTKKMSKSDTNVNSFILMTDDKDA
                     IVRKFKRAVTDSDGVVRFDRETKPGVSNLMCIYSTFTGKSNDEIAAEFEGKGYGDFKL
                     AVAEVTADALAPVQAEYGRILADKAYVDEVLKNGAERASRLANRTVSKVYRKVGLLQL
                     DK"
     gene            1346..1834
                     /gene="greA"
                     /locus_tag="ACZPAX_09740"
     CDS             1346..1834
                     /gene="greA"
                     /locus_tag="ACZPAX_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HIR1114218.1"
                     /translation="MAKEVVVTREGYNKLEQELENLRTVKRKEVADKIKVARGYGDLS
                     ENAEYDAAKEEQASVEARIADLEATLKLARIMDESELSNDTASIGMHVKILAEGDDLE
                     DAEEYDLTGSTEADMDLNRISDESPVGAAMIGCKVGDSFNVTLPNGNIITYKLLEVSR
                     SK"
     gene            1873..>2147
                     /locus_tag="ACZPAX_09745"
     CDS             1873..>2147
                     /locus_tag="ACZPAX_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HIR1114219.1"
                     /translation="MEQNKPQTEQEISQQAAVRRQKLADLCEAGHNPFEITKYAQDTY
                     SADLKQEFADLPAEAETGKIVSLAGRMMSKRVMGKASFAHLRDQKGD"
BASE COUNT          509 a          639 c          605 g          394 t
ORIGIN      
        1 acacgaaaac acatgaactg cctagtcctg cgtgaaaatt tgcgtgggag aacggttcgg
       61 caaggaggcg gtcaatggca tcaccgtata tcccttgcag gtgtctgccg ttgagacggc
      121 tgctctgcgc tggatgcgca cgatggcggc gacggaatac gaataaagca tattcttccc
      181 ttgtattgaa aaagtgattt ataacgaatg gagataaagt accatggcag aagaaaaacg
      241 caaacgcatt ctttcgggca ttcagcccac cggcacgccc actctgggca actatatcgg
      301 cgcggtgcgc aactgggcac tgctgcaatc tgactatgac tgcctgtaca tggtggccga
      361 cctgcacagc ctgaccgtgc gccaggtgcc tgccgacctg cgccgccgca cccgtgagct
      421 ggccgccctg ctgctggccg tgggcatcga cccgaaggag cacgttctgt tcgtgcagag
      481 ccatgtgccg gcgcatacag agctttcctg ggtgctggcc tgcaacaccc agttcggcga
      541 gctgtcccgc atgacccagt tcaaggacaa gagcgccaag caccccgaca acgtcaacgg
      601 cggcctgttc acctaccctg tgctgatggc ggcggatatc ctgatttata acgcagacct
      661 cgttcccgtg ggccaggacc agacccagca cctggagatt gcccgcgata tcgcaggccg
      721 cttcaacggc atctacggcg acactttcac cctgccggag ggctacgtcc ctgccgccgg
      781 cgcgaaaatc atgtcgctgg ccgaaccgac taaaaagatg agcaagtccg acacgaacgt
      841 caacagcttc atcctgatga ccgacgacaa ggacgccatc gtgcgcaagt tcaagcgcgc
      901 cgtgaccgac tccgacggcg ttgtccgctt tgaccgtgag acgaagcccg gcgtgtccaa
      961 cctgatgtgc atttacagca cctttaccgg caagtccaac gatgagatcg ccgccgagtt
     1021 tgaaggcaag ggctatggcg acttcaaact ggctgtggcc gaagtcaccg ccgatgcgct
     1081 ggcccccgtg caggccgagt atggccgtat tctggccgac aaagcctatg tggatgaggt
     1141 gctcaagaac ggcgccgagc gagcctcccg cctggccaac cgcaccgtca gcaaagttta
     1201 ccgcaaagtt ggcctgctgc agctggataa atgacgttgt gacttgacaa atcccattta
     1261 aacctatata atatctaagt tggtgcggct ccccagttgg tgccgcacta cttttactat
     1321 ccattttacg ttaggagaca ctgctatggc taaagaagtt gtcgttaccc gcgagggcta
     1381 taataaactg gagcaggaac tggaaaacct gcgcaccgtc aaacgtaagg aagtcgctga
     1441 caagatcaag gtcgcccgcg gctacggcga tttgagcgag aatgccgagt acgacgccgc
     1501 caaggaggag caggcttctg tggaggcccg catcgccgac ctggaggcca ccctgaagct
     1561 ggcccgcatc atggatgaga gtgagctgtc caacgacacg gcttccatcg gtatgcacgt
     1621 caagattctg gccgagggcg atgacctgga ggacgccgag gagtacgacc tgaccggctc
     1681 caccgaggcg gatatggacc tgaaccgcat cagcgacgag agcccggttg gcgctgccat
     1741 gatcggctgc aaagtcggcg acagctttaa tgtcaccctg cccaacggca atatcatcac
     1801 ctacaagctg ctggaagttt cccgttccaa gtaagcattt gaaatcaaaa aatcacgaaa
     1861 ggacgatctc acatggagca gaataagccc cagaccgagc aggaaatcag ccagcaggcc
     1921 gccgtgcgcc gccaaaagct ggctgacctg tgcgaagccg gtcacaaccc ctttgaaatc
     1981 acaaaatatg cgcaggatac gtactcggcc gacctcaagc aggagtttgc cgacctgccc
     2041 gccgaagccg agaccggcaa gatcgtctct ttggccggcc gcatgatgtc caagcgcgtc
     2101 atgggcaagg ccagctttgc ccatctgcgc gaccagaagg gcgacat
//
LOCUS       DBMDSQ010000340         2144 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_11305_length_2144_cov_1.945668, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000340 DBMDSQ010000000
VERSION     DBMDSQ010000340.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2144)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11305_length_2144_cov_1.945668"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(32..107)
                     /locus_tag="ACZPAX_09750"
     tRNA            complement(32..107)
                     /locus_tag="ACZPAX_09750"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(72..74),aa:Gly,seq:gcc)
     gene            complement(214..1125)
                     /gene="hslO"
                     /locus_tag="ACZPAX_09755"
     CDS             complement(214..1125)
                     /gene="hslO"
                     /locus_tag="ACZPAX_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HIR1114220.1"
                     /translation="MEQNHNMLRGISENGGIVFYGVDSTEIVREMERLHKTSAVTTAA
                     LGRLLTAASMMGIMLKSTQDSVTLQIKGGGPAGRLLAVSDGTGNVKGYVEHPVVELPP
                     RADGHLNVGAAVGKDGTLDVIRDLGMREPYIGQVPLTSGEIAEDVTTYFAISEQVPTV
                     CALGVLVDKDLSVRCAGGFIVQLLPGATEEEIEHLEKNIKAMPSVTTMLEEGKSVRDM
                     LELALAGFQPDILDSYHVTYKCDCSLERVEKMIRSLGKKKVERMRDEDPIAEVNCQFC
                     NKNYKVDLNELLKNWPAIDEKPADENA"
     gene            complement(1126..1884)
                     /locus_tag="ACZPAX_09760"
     CDS             complement(1126..1884)
                     /locus_tag="ACZPAX_09760"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HIR1114221.1"
                     /translation="MAYNEFAYFYDEFNGEADYDALYGYVTDELKAHGVADGILADLG
                     CGTGDLTLMLTQAGYDVIGIDRSEEMLSVLREKADELGLTGRLLLLKQDLLDLDLYGT
                     IRAAVSTFDTYSHIGPLDNFEKAIRKAAYFMEKGGVFIFDLNTPYKHQKILAGQTFDI
                     EAEDADCHWSNRYDEAAQRVDITIDIDYHETGEHFREQFSEYSYALDTVTALLEKYGF
                     AVAKMADGESFGPVRADSPRWIFTAVKQYTQEVQ"
     gene            complement(1888..>2144)
                     /locus_tag="ACZPAX_09765"
     CDS             complement(1888..>2144)
                     /locus_tag="ACZPAX_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005924021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HIR1114222.1"
                     /translation="RHAPSADDVQMLEYLHYHRIPFVAALTKGDKLKKSEMATRKEEF
                     ERICAPYGCQAVVLTSAENGLGMDELRALLEASMAVEDED"
BASE COUNT          405 a          638 c          592 g          509 t
ORIGIN      
        1 gctctcccag ctgagctaaa cttccccata atggagcggc cgacgaggct cgaactcgct
       61 acctccacct tggcaaggtg gcgctctacc agatgagcta cggccgcata ttggagagtt
      121 tttgctcagt cgctctctcc gggcgacgca tgttattata tactatgtga gcagaaaagt
      181 caagcgtttt ttgcgaactt ttttcaaagt tttttatgcg ttttcgtccg caggtttttc
      241 atcgatggca ggccagtttt tgagcagttc gtttaggtcc accttataat ttttgttgca
      301 gaactggcag tttacctcgg caatggggtc ctcgtcgcgc atacgctcga cctttttttt
      361 gccaagggag cggatcattt tttccacacg ctccaagctg cagtcgcact tgtaggtcac
      421 gtggtagctg tccaggatat cgggctggaa accggccagg gccagctcca gcatatcgcg
      481 gacgctcttg ccctcttcca gcatcgtagt cacactgggc atggccttga tgttcttttc
      541 cagatgttcg atttcttcct cggtcgcacc gggcagcagc tgcacgatga aaccgcctgc
      601 gcagcgcaca ctcaggtctt tatccaccag cacgcccagg gcgcagacag tggggacctg
      661 ctcactgatg gcaaagtagg tcgtgacgtc ctcggcaatc tcgccggagg tcagcggcac
      721 ctgtccgata tatggctcac gcatgcccaa atcgcggata acatccagcg tgccgtcttt
      781 gccgacggct gcgcccacgt tcaggtggcc gtccgcccgc gggggcagct ccacgactgg
      841 gtgctccaca tagcccttga cattgccggt accgtcggag acagccagca aacggcctgc
      901 ggggccgccg cccttgatct gcaaagtcac cgagtcctgg gtgcttttga gcataatgcc
      961 catcattgac gcggcggtca gcaggcggcc cagcgcggcg gtggtaacag cactggtctt
     1021 gtgcaggcgc tccatttcgc ggactatttc cgtagaatct acgccgtaaa atacgatgcc
     1081 gccgttctcg gagataccgc gcagcatatt atgattctgt tccatttatt gtacctcttg
     1141 tgtatattgc ttgacagccg tgaagatcca gcgcgggctg tcggcgcgta cagggccgaa
     1201 gctctcgccg tcggccattt tagccactgc aaagccgtac ttttccagca gggcggtcac
     1261 ggtgtccagc gcatagctgt actcgctgaa ctgctcacgg aagtgctccc ctgtttcgtg
     1321 gtagtcgata tcgatggtga tatccacccg ctgtgcggcc tcgtcatagc ggttcgacca
     1381 atggcagtcg gcgtcctcag cctcaatgtc aaaagtctgc ccggccagga ttttttggtg
     1441 cttgtagggg gtgttcaaat cgaagatgaa cacgccgccc ttctccataa aataggccgc
     1501 cttgcggatg gccttttcaa aattgtccag cgggccgatg tggctgtacg tgtcaaaggt
     1561 ggacaccgcc gcgcggatag tgccgtacag gtccaggtct agcaggtcct gttttaacag
     1621 cagcagacgg ccagtcaggc ccagctcgtc ggctttctcc cgcaggacgc tgagcatttc
     1681 ctccgagcgg tcgatgccga tgacatcgta cccggcctgg gtcagcatca gcgtcaaatc
     1741 accggtaccg cagcccaggt ctgccaggat gccgtcggca acaccatggg ctttcagctc
     1801 atcggtcaca tagccgtaca gcgcgtcgta gtcggcctcg ccgttgaact catcgtaaaa
     1861 ataggcaaac tcgttgtagg ccatcggtca atcctcgtcc tctactgcca tgctggcttc
     1921 cagcagagcg cgcagttcgt ccatacccag accgttctcg gcgctggtca gcacaacggc
     1981 ctggcagccg taaggggcgc agattctttc aaattcttcc ttgcgggtgg ccatctcgct
     2041 ctttttcagc ttatcgccct tggtcagcgc cgccacaaag ggaatgcggt ggtagtgcag
     2101 gtactcaagc atctgcacat cgtcggcgct cggggcatgg cggc
//
LOCUS       DBMDSQ010000341         2143 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_11314_length_2143_cov_1.985798, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000341 DBMDSQ010000000
VERSION     DBMDSQ010000341.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 2143)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2143)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2143
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11314_length_2143_cov_1.985798"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            189..1634
                     /locus_tag="ACZPAX_09770"
     CDS             189..1634
                     /locus_tag="ACZPAX_09770"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HIR1114223.1"
                     /translation="MLDVKRNLTTMTDFYELTMSAGYLDEGYVDKIAVFDMFFRRVPD
                     GGGYAIMAGLQQFIDAVDHLKFTAEDIDYLRSTKAFDEKFLDYLKNFKLHCNIWAIEE
                     GMPIFPQEPIVTVEGPAIECQLLETLLLVTFNHQCLIATKANRIVRSAAGRPVMEFGA
                     RRAQGYDAAYFGARASYIGGCGSTSCVMAARDFGIPASGTMAHSWVQMFPTEYDAFKK
                     YAEMYPDACVLLVDTYNVLRHGVPDAIKVFDEVLKPMGKRPKGIRIDSGDIAYLSKKA
                     RKMLDEAGYPDCTICASNSLDEYIVRDLILQGARVDSFGIGENMITAKSDPVFGGVYK
                     LAAVKEDDGSYTPKMKLSESAEKMTIPCLKKVWRIYDEDGKAMADLITMADEQVETLH
                     GITLFDPIETWKECTYVNCTARCLSTPIYENGKRVYNSPSLDDIKKFCKAQVGTLWDE
                     VKRFENPHRYYVDLSQKLWDTRSTLLKKLSK"
     gene            1719..2114
                     /locus_tag="ACZPAX_09775"
     CDS             1719..2114
                     /locus_tag="ACZPAX_09775"
                     /inference="COORDINATES: protein motif:HMM:NF018271.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease"
                     /protein_id="HIR1114224.1"
                     /translation="MLERLSLFCLGGGAYAALELAWRGTTHWTMFLAGGVCLCLLQAL
                     AAGPLPLVVAAALGAAGVSGLELAVGAVCRRLLHIAVWDYADEWGNLAGLVCPRYTFY
                     WFLLCGWVVFVLRGVESTVYHPQKSVQKS"
BASE COUNT          429 a          630 c          616 g          468 t
ORIGIN      
        1 cacgattacc cgggtgagcc tttttttttc aagcacatat ttcaaagtcg tcgttatcct
       61 gaccatgctc gtcatcggtg cttacctgat tttcaccgtt ggccgcatcc tgcgcatctg
      121 gagcaaagag gtctgaatat aggcgtgccg cccggtgcgc tgcaaaattc cacaggaggg
      181 tttctaccat gttggatgta aaacgcaatt tgaccactat gaccgatttc tatgagctga
      241 ccatgtcggc gggctatctg gatgagggat atgttgacaa gatcgccgtc tttgatatgt
      301 tcttccgccg tgtgccggat ggcggcggct acgccatcat ggcgggcctg cagcagttca
      361 ttgacgccgt ggaccacctg aaatttaccg ccgaggatat cgactacctg cgctccacga
      421 aggcgttcga cgaaaaattc ctcgactacc tgaagaattt caagctgcac tgcaacatct
      481 gggccattga ggaaggtatg cccatcttcc cgcaggagcc gatcgtcacc gtcgaaggcc
      541 cggccatcga gtgccagctg ctggaaacgc tgctgctcgt caccttcaac caccagtgcc
      601 tgattgccac caaggcaaac cgcatcgtgc gctctgccgc aggccgcccg gtcatggagt
      661 tcggcgcccg ccgcgcccaa gggtatgacg ccgcctattt cggcgcacgc gcctcttata
      721 tcggcggctg cggctccacc tcctgcgtca tggccgcccg cgatttcggc atcccggcct
      781 ccggcaccat ggcccacagc tgggtgcaga tgttcccgac tgagtatgat gctttcaaga
      841 aatacgccga gatgtacccc gacgcctgcg tgctgctggt ggacacctac aatgtgctgc
      901 gccacggcgt gcccgatgcc atcaaggtat ttgatgaggt cttgaagccg atgggcaagc
      961 gcccgaaggg catccgcatt gactccggcg atatcgccta cctgtccaag aaggcccgca
     1021 agatgctgga cgaggccggc taccccgact gcacgatttg cgcatccaac tctctggatg
     1081 agtacatcgt ccgcgacctg attttgcagg gtgcccgcgt ggacagcttc ggtatcggcg
     1141 aaaacatgat caccgccaag agcgaccctg tgttcggcgg cgtgtacaag ctggccgccg
     1201 tgaaagagga cgacggcagc tacacgccga agatgaagct gtccgaatcc gccgagaaaa
     1261 tgacgatccc ttgcctgaaa aaggtttggc gtatctacga cgaggacggc aaggccatgg
     1321 ccgacctcat cacgatggcc gatgagcagg tcgagaccct gcacggcatc acgctgtttg
     1381 accccatcga gacgtggaag gaatgcacct acgtcaactg cacggctcgc tgcctgtcca
     1441 ccccgattta tgagaacgga aagcgcgtct acaactcccc gagcctggat gatatcaaga
     1501 agttctgcaa ggcccaggtg ggcacgctgt gggacgaagt caagcgtttt gagaacccgc
     1561 accgctacta cgtcgacctg agccagaagc tgtgggatac cagaagcaca ctgctgaaga
     1621 agctgtcgaa gtaaaatttc ccactatcct tacaaaacgg ttacaaccat cactccctgc
     1681 acatactgtt gctaacagct tgtgcagggg gattttgtat gttggaacgt ctttctctgt
     1741 tttgtctggg tggcggggcc tatgccgcac tggaactggc ctggcgtggc accacccact
     1801 ggacgatgtt tctggcgggc ggggtctgcc tctgcctttt gcaggcactg gccgccgggc
     1861 cgctgccgct ggtggtcgcg gcggccctgg gcgcggcggg cgtcagcggg ctggagctgg
     1921 ctgtgggcgc ggtctgccgc cgccttttgc atatcgccgt ctgggattat gccgatgagt
     1981 ggggcaacct ggcgggccta gtctgcccac gctacacctt ttactggttt ttgctctgcg
     2041 ggtgggtcgt tttcgtgctg cggggcgttg aaagtaccgt ttatcaccct caaaaatcgg
     2101 tacagaagag ttaaagtcca aattatcgta ctttccgtct ggt
//
LOCUS       DBMDSQ010000342         2130 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_11411_length_2130_cov_1.695416, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000342 DBMDSQ010000000
VERSION     DBMDSQ010000342.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2130)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2130
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11411_length_2130_cov_1.695416"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..347)
                     /locus_tag="ACZPAX_09780"
     CDS             complement(<1..347)
                     /locus_tag="ACZPAX_09780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1114225.1"
                     /translation="MKIPAKKRTALELLAAVLWCLVTLGTDRLFFKYDWKTPAFFVYK
                     ALFVLLAFLLVHGVVTLVQKVRAGNSFARRWLAWTLPYLAVNLVVLLIVWPGIWGNDD
                     LAVLSLARTLQPNS"
     gene            complement(363..1115)
                     /locus_tag="ACZPAX_09785"
     CDS             complement(363..1115)
                     /locus_tag="ACZPAX_09785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007048316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HIR1114226.1"
                     /translation="MSKLHLILPMAGRGSRFFENGFVCPKPLIEIGGKPFFYWATRSV
                     EKYVDCADITFVVLEEHIRDFGIDAKIKQYWPEARIIALPEVTEGAAITALKGCEGLP
                     DGEPILFNDCDHLFICSAFNAFCEKGDFADGPAGALLTFESDSPAYSYLQYGADGNVC
                     HTVEKQVVSHDAICGAYYFKDKKTYADACAEYLKNCEYKEFFVSGIYNVLAAQGAKIA
                     GFACDLHLPFGTPDEYRTAEAPENRAGFDELV"
     gene            complement(1331..>2130)
                     /locus_tag="ACZPAX_09790"
     CDS             complement(1331..>2130)
                     /locus_tag="ACZPAX_09790"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007048315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR1114227.1"
                     /translation="YDFATRGLFYRCEYKRSEYPTAIRPAFPRECARRTGVKQVVQAS
                     TNAMYENETEFPTVESKFNPPTLIYPNTKYCAERFAQSFADTYGMTVTCLRFANVYGP
                     HIDCLRKQPPFVGYMIRELYYDRTPEFHSDGNQRRDYIYVDDLIALALRVVERPQKGF
                     DAVNVSSNQSYSVRELYAIANKLMGKNIEAKYCPSSHYWAKYPELYGGAYGIKPEILD
                     HEVNKFSQCDNTHAREAYGWEPKVDIETGLSRVVEAETKMLAEKDKR"
BASE COUNT          437 a          632 c          576 g          485 t
ORIGIN      
        1 gagtttggct gcagcgtccg cgccagagac agcaccgcca agtcgtcgtt gccccagata
       61 ccgggccaga cgatgagcag caccaccaag ttcacggcca ggtacggcag cgtccaggcc
      121 agccagcgcc gggcaaagct gttccccgcc cgcactttct gcaccagcgt cacaacgccg
      181 tggaccagca aaaacgccag cagcacaaac aacgccttgt agacgaaaaa tgccggcgtt
      241 ttccagtcat atttaaaaaa cagcctgtcc gttcccagcg tcaccaggca ccacagcacc
      301 gccgccaaca gttccagcgc cgtccgcttt tttgcaggta tcttcattcg tactcctttg
      361 cgtcagacaa gctcgtcaaa cccggcgcga ttttcaggcg cttcggcggt gcggtactcg
      421 tccggcgtgc cgaaaggcag gtgcaggtcg caggcaaagc ccgcgatttt tgcgccctgc
      481 gccgccagca cattgtagat gccggagaca aaaaactcct tgtattcgca gtttttcagg
      541 tactcggcgc aggcgtcggc gtaggttttt ttgtctttga aatagtacgc tccacaaatc
      601 gcgtcgtggc tgacgacctg cttttccacc gtgtggcaga cattgccgtc cgcgccgtac
      661 tgcaagtagc tgtatgccgg ggaatcgctc tcaaacgtca gcagcgcccc cgccgggccg
      721 tcggcaaagt cgcccttttc gcagaacgca ttgaacgcgc tgcagataaa caggtggtcg
      781 cagtcattga acaggatcgg ctcaccgtcg ggcagcccct cgcagccctt cagcgccgtg
      841 atcgccgcac cctcagtcac ttctggcagg gcaatgatgc gtgcctcggg ccaatactgt
      901 ttaattttgg cgtcgatgcc aaagtctcgg atgtgttctt ccagcacaac aaaggtgata
      961 tcggcgcagt ccacatattt ttccacgctt cgcgtcgccc agtaaaagaa cggcttgccg
     1021 ccaatttcaa tcagcggttt cgggcagacg aacccatttt caaaaaatcg gcttccccgc
     1081 ccggccatgg gtaaaatcaa atgcagcttg ctcataagaa gctccttttt agataaaaga
     1141 taatagataa taaaaatggt ggttcgcggt gccggcgcat cgcttcaaaa tttctctacg
     1201 accatagggg cggattccat atgcgcccgt tctgctttgg gcaacgccgc gggcggatat
     1261 ggaatccgcc cctacaattt tgttttgacc ttcaggtcaa aactccacat tttttattct
     1321 cttttatcta ttatctctta tctttttccg ccagcatctt cgtctcggcc tcaacgaccc
     1381 ggctcaagcc ggtctcaata tccaccttcg gctcccagcc gtaggcttcg cgggcgtggg
     1441 tgttgtcgca ctggctgaac ttgttgacct cgtgatccag aatctccggt ttgatgccgt
     1501 aagccccacc gtacagctcg gggtacttgg cccagtaatg gctgctgggg cagtatttgg
     1561 cctcgatgtt cttgcccatc agcttgttgg caatggcgta cagttcccgc accgagtagc
     1621 tctggttgct ggacacattc accgcgtcaa agcccttctg ggggcgctcc accacgcgca
     1681 gcgccagcgc gatcaggtca tccacataaa tataatcccg gcgctggttg ccgtcggagt
     1741 ggaactccgg cgtgcggtca tagtacagct cgcggatcat gtagcccaca aacggcggct
     1801 gcttgcgcag gcagtcgatg tgcgggccgt agacgttggc aaagcgcagg caggtcacgg
     1861 tcatgccata agtgtcggca aagctttggg caaagcgctc ggcgcagtat ttggtgttgg
     1921 ggtagatcag ggtgggcggg ttgaacttgc tttccacggt cgggaactcg gtctcgttct
     1981 cgtacatggc gttggtggag gcctgcacca cctgttttac gccggtgcgg cgggcacatt
     2041 ccctggggaa cgctggccgg atggctgttg gatattccga ccgcttatat tcgcagcgat
     2101 aaaaaagacc acgggttgca aaatcgtatc
//
LOCUS       DBMDSQ010000343         2120 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_11484_length_2120_cov_1.484398, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000343 DBMDSQ010000000
VERSION     DBMDSQ010000343.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 2120)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2120)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2120
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11484_length_2120_cov_1.484398"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..695
                     /locus_tag="ACZPAX_09795"
     CDS             <1..695
                     /locus_tag="ACZPAX_09795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1114228.1"
                     /translation="PAPENAEAVTAYLRQMGVREGNILVDIGSGGTTQLLLERLLGVQ
                     LHGLQLSADERLRSRFDETRTEVFLFGGQPAPRLYWAGQPMLERLISEDVGATLGYRA
                     AEDKIEAVAASQPVEPLLAGIQQGVRNFAVAWRDSVLHDWPIPPEQAIAPFLQLVESP
                     TALQVKLLGDLTVEDGGVYPLAAPQSAAHYLAHPRDVKRDLAEARWKIGFLKRLAPLP
                     LPYGKIYLKLKK"
     gene            715..1731
                     /locus_tag="ACZPAX_09800"
     CDS             715..1731
                     /locus_tag="ACZPAX_09800"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR1114229.1"
                     /translation="MAKISVILPAYNAEKTLRTAVESILSQQVPGMEIIIVNDGSADG
                     TARLCHVLASENDCIHVITQKNAGICAARNRGLEAAGGEFIAFCDDDDLFWQGALKLL
                     LQTAEDSHADIVRGGYELLRQRPDGTFAEQRHPTGQPCTIELGKGGYGAFLENSGPQF
                     VWNALYRRTALLGLRFNERCSFGLEDFVFNAAAYRRICKAVYIPQVVYRHFEGAQSTS
                     CARTAQALMGRIRALEPWMEAEFHAAQRWCSPDEMQAVWKNRRAQAVTFLMHQLRDAR
                     APDPLRRQAWRTLREVLAPYPGGMLDILHDAGNNKKQTAALLLYQLRLQNLYDLLTIR
                     EEQL"
     gene            1728..>2120
                     /locus_tag="ACZPAX_09805"
     CDS             1728..>2120
                     /locus_tag="ACZPAX_09805"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR1114230.1"
                     /translation="MKTILVTGAAGYIGRHVVKNALDRGYKVIAADFNFKGVDERAEF
                     CDVPLFNGDADLYRKLGSPDICIHLAWRDGFRHNSLAHMKDLSSHIVFLNALIDGGLK
                     DLSIMGTMHEVGYWEGAINESTPCKPQSQ"
BASE COUNT          431 a          644 c          656 g          389 t
ORIGIN      
        1 tgcccgcccc ggaaaacgcc gaggccgtga ccgcctacct gcgccaaatg ggcgtgcggg
       61 agggaaatat cctggtggat atcggaagcg gcggcacgac ccagctgctg ctggaacggt
      121 tgctgggcgt gcagctgcac ggcttgcagc tctcggcaga tgagcgcctg cgcagccgct
      181 ttgacgagac gcgcaccgag gtgttcctgt tcggcggtca gcccgctccg cgcctgtact
      241 gggcaggtca gccgatgctg gaacgactca tttctgagga tgttggcgcg acgctcggct
      301 atcgggcggc agaggacaaa atagaagcag tcgcggcgtc ccagcctgtc gagccgctgc
      361 tggcgggtat ccaacagggc gtgcggaact tcgccgttgc ctggcgggac agcgtgctgc
      421 atgattggcc catcccgccg gaacaggcca ttgcaccatt tttgcagctg gtggaaagcc
      481 cgactgcttt acaggtaaaa ttgctgggag atttgactgt cgaggatggc ggtgtgtatc
      541 cgctggccgc cccccagagc gccgcccact acctggccca ccccagggac gtaaaacgcg
      601 atctggcaga agcgcggtgg aaaatcggct ttttaaagcg gctggcccca ctgccgctgc
      661 cctacggaaa aatctatctg aaactcaaaa aataatcagg aaaggagaga taccatggca
      721 aaaatcagcg tcattttacc ggcctacaac gccgaaaaga ccctgcgcac cgccgtggag
      781 tccatccttt cccagcaggt gccggggatg gaaatcatca tcgtcaacga tggctccgcc
      841 gacggcacgg cccggctctg ccatgtgctt gccagcgaga acgactgcat ccacgtcatc
      901 acccagaaaa acgccggcat ctgcgcggcc cgcaaccgcg ggctggaagc cgcgggcggc
      961 gagtttatcg ccttctgcga cgacgatgat ctgttctggc agggggcact taaactgctt
     1021 ttgcagactg cagaggattc ccacgccgac attgtgcgcg gcgggtatga gcttttgcgc
     1081 cagcgcccgg acggcacctt tgccgagcag aggcatccca ccgggcagcc ttgcacaata
     1141 gaattgggca agggcggcta tggtgccttt ttggagaaca gcggcccgca gtttgtctgg
     1201 aacgcccttt accgccgcac agcgctgttg ggcctgcgct ttaatgagcg gtgcagcttc
     1261 ggcctggagg atttcgtctt taacgctgcg gcctaccggc gcatctgcaa ggcggtctac
     1321 atcccgcagg tagtctaccg ccactttgag ggagcgcaga gcacctcctg cgcgcggacg
     1381 gcgcaggcac ttatggggcg catccgggcg ttggaaccct ggatggaggc ggaatttcac
     1441 gccgcccagc gctggtgcag ccccgacgaa atgcaggctg tgtggaaaaa ccgccgcgcc
     1501 caggccgtga catttttgat gcaccagctg cgggatgccc gcgcccccga tccgctgcgt
     1561 cggcaggcgt ggcgcaccct gcgggaagtt ctggccccat acccaggcgg aatgcttgac
     1621 attttgcacg atgcagggaa caataaaaag cagacggccg cgctgctctt ataccagctg
     1681 cggctgcaaa atctgtacga tttgctgacc atcagagagg aacaactatg aaaacgattc
     1741 ttgtcaccgg cgcggccggg tatatcggcc gccatgtggt caaaaatgcc ctggatcgcg
     1801 gctataaggt cattgcggca gacttcaact ttaaaggcgt ggacgagcgc gccgagttct
     1861 gcgatgtgcc gctgttcaac ggcgacgctg acctgtaccg caaactgggc agcccggata
     1921 tctgcatcca tctggcctgg cgcgacggct tccgccacaa ttcgctcgcc cacatgaagg
     1981 atctgtccag ccatatcgtg ttcctgaacg ccctcatcga cggcggcctc aaagatttga
     2041 gcatcatggg cacgatgcac gaggtcggct actgggaggg ggctatcaac gagagcacgc
     2101 catgcaagcc ccagagccag
//
LOCUS       DBMDSQ010000344         2114 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_11524_length_2114_cov_1.526080, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000344 DBMDSQ010000000
VERSION     DBMDSQ010000344.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 2114)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2114)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2114
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11524_length_2114_cov_1.526080"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..595
                     /locus_tag="ACZPAX_09810"
     CDS             <1..595
                     /locus_tag="ACZPAX_09810"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HIR1114231.1"
                     /translation="VEDADLDFGKTETVQAGQSGSEQVTSEIVTVDGAVVSCQAVDVQ
                     LLQAPVPEIIHRGTRLKSGMIGKLGTGSFLWPVPGYSGISRWANLPYGHRGVDITAPY
                     GTPIYAADAGTVIAAQWHNHPTASWGYYVEIDHGNGYKTLYGHMSSFVVQTGQTVTKG
                     QLIGYVGATGTATGNHCHFEMYYNNALISARNVFPDI"
     gene            783..1229
                     /locus_tag="ACZPAX_09815"
     CDS             783..1229
                     /locus_tag="ACZPAX_09815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HIR1114232.1"
                     /translation="MDHIDRKIIDILQHDARIPLKEIADQVFLSSPAVSARIARLEHS
                     GTITGYQAQVDAPKLGYMVKAFINLDMDPQRKPEFYPYIESCAHVVECNCVTGDYSML
                     IEVLFGTTQELDQFINHLQQFGRTRTQIVFSTPVEHRGVPVAEPEE"
     gene            complement(1270..>2114)
                     /locus_tag="ACZPAX_09820"
     CDS             complement(1270..>2114)
                     /locus_tag="ACZPAX_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HIR1114233.1"
                     /translation="FCRASDKLTFKITLPIMLFLDMGSVDILHDFQPKFVLFCFGATF
                     IGILVVWAGAKRFLKDKSLVGEFVQAGYRSSAAVLGVAFIQNIYGNAGMAPLMILGSV
                     PLFNIFAVLILMLESPQQRGIPTPKQLLRGVATNPILLGIVSGTVYALLPFTLPAVLT
                     KTLNNIASVTTPLALLSIGASFEGAKAIKKLGPTLAAAFIKTVGLAAVFLPCAVALGF
                     RDEELIALLIMLGSPTTPSAYVMSKNMGHEGVLTSSCIALTTLLSALTLTGWIFLLRS
                     GGYL"
BASE COUNT          474 a          623 c          609 g          408 t
ORIGIN      
        1 cgtcgaggac gctgacctgg attttggcaa gaccgagacg gtacaggccg ggcagagcgg
       61 cagcgagcag gtcacaagcg aaattgtgac cgtggacggc gcggtcgtca gctgccaggc
      121 cgtggacgtg cagcttttgc aggcccctgt gccggagatc atccaccggg gcacacggct
      181 gaaaagcggc atgatcggca agctcggcac cggcagcttt ctctggcctg tgccggggta
      241 ttcgggcatc agccggtggg ctaatctacc ctatggccac cgcggggtgg acatcaccgc
      301 gccctacggc acgccgattt acgccgccga cgcgggcacg gtcatcgctg cccagtggca
      361 caatcaccct accgcgagct ggggctatta tgtggagatt gaccacggca acggctacaa
      421 gacgctgtac ggccacatgt cgtcctttgt ggtgcagacc gggcagaccg tgacgaaggg
      481 ccagctgatc ggctacgtgg gggcgactgg caccgccacc ggcaaccact gccattttga
      541 aatgtattat aacaatgcac tgatcagcgc aagaaatgtg ttcccggata tataactgcc
      601 tgccagaaca ctgcgggcgg atgtgagcat ccgcccctgc aattctacaa ttcttttgca
      661 aaacgcttta ctttttactt tgtatatggt aaaattcttt ccatagtaaa gctacaaaag
      721 ctctcgttca ggtgcaatga cgcacacgaa cgggagcttt ttgcctaaaa tgaggtgtta
      781 ccatggatca catcgaccgc aagatcattg acatattgca gcacgacgcg cgtattcccc
      841 taaaagaaat cgctgaccag gtatttttgt ccagccctgc ggtgtcggcc cgcattgccc
      901 ggctggagca ttccggcacc atcaccggct atcaggccca ggtggacgca cccaagctgg
      961 gctacatggt caaggcgttc atcaacttgg acatggaccc ccagcgcaag ccggagttct
     1021 acccctatat tgagagctgc gcccatgtgg tggagtgcaa ctgcgtcacc ggcgattaca
     1081 gtatgctgat tgaggttttg ttcggcacga cgcaggaatt ggaccagttc atcaaccact
     1141 tgcagcagtt tggccgcacg cgcacgcaga tcgtgttctc gaccccggta gagcaccgcg
     1201 gtgtgccggt ggcggagccg gaggaataaa aaacgccttt ctcaatgggg gggtgaagcc
     1261 tttgggatat tacaggtatc ccccgctgcg cagcaggaat atccagccgg tcagcgtcag
     1321 tgccgagagc agcgtcgtca gggcgatgca gctcgacgtc agcacaccct cgtggcccat
     1381 gtttttgctc atgacatagg cgctgggagt cgtggggctg cccagcatga tgagcagcgc
     1441 aatcagttcc tcgtcgcgga accccagcgc caccgcgcag ggcagaaata ccgccgccag
     1501 ccccacggtc ttgataaagg ccgccgccaa cgtcggcccc agctttttga tggccttggc
     1561 cccctcaaac gaggcaccaa tggacagcag cgccaacggc gtagtgacgg aggcaatatt
     1621 gttcaacgtt ttggtcagca ctgcgggcag cgtgaacggc agcagcgcat agaccgtgcc
     1681 ggacacgata cccaataaaa tcgggttggt ggccacgccg cgcagcagct gctttggcgt
     1741 ggggataccg cgctgctgcg gggattccag catcaaaatc agcaccgcaa agatgttgaa
     1801 cagcggcacg ctgcccagaa tcatcagcgg ggccatgcct gcgttaccgt agatgttctg
     1861 gatgaacgcc acgcccagca cagccgcact gctgcggtaa cccgcctgca cgaactcccc
     1921 caccagcgat ttatctttga ggaaccgctt tgcccctgcc cacacaacaa ggatgccgat
     1981 gaacgttgcg ccaaagcaga acagcacaaa cttcggctgg aagtcgtgca ggatgtcaac
     2041 gctgcccata tccagaaaca gcatgatggg cagtgtgatt ttaaaggtca gcttatccga
     2101 cgccctgcaa aatt
//
LOCUS       DBMDSQ010000345         2088 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_11705_length_2088_cov_1.511324, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000345 DBMDSQ010000000
VERSION     DBMDSQ010000345.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2088)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11705_length_2088_cov_1.511324"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..690
                     /locus_tag="ACZPAX_09825"
     CDS             <1..690
                     /locus_tag="ACZPAX_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015537257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HIR1114234.1"
                     /translation="KTVFVENRVRQMIHANLDRIEDVKIVQNGPGAAGTAIIHMLQVA
                     GAKHIIAVDEHGILVPGRPAGLEGHKGKLAEETNPEKLSGGLAEAIKDADIFIGTSIA
                     GALTPALAATMAKDAIVFAMANPTPEIMPDLAKQAGIRVIGTGRSDFPNQVNNVLAFP
                     GIFKGALSVRARDINPAMKMAAAKAIAGLIPDDELNEENILPKAFDPRVADAVAAAVA
                     QAARESGVARV"
     gene            693..1532
                     /locus_tag="ACZPAX_09830"
     CDS             693..1532
                     /locus_tag="ACZPAX_09830"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005921212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HIR1114235.1"
                     /translation="MREISAAAITDAVEALCIEANTQLPADVKAALDKAEANEPWPLA
                     KETLGLLQQNLCVAADKDLPICQDTGMACVFIELGQDAHINGDLTDAVNEGVRRGYEK
                     AYLRKSITADPLQRVNTGDNTPAFLTVHLVPGSGCKITVAPKGAGSENMSRLAMLKPA
                     DGVEGVKKFVLDTVEQAGANPCPPVVLGIGIGGSFDHCAALAKKALLRPLDTPNADPY
                     YAALEKELLDAINATGFGPQGFGGATTCLGVAIEQKPTHVACLPVAVNISCHVTRRAS
                     REV"
     gene            1536..1751
                     /locus_tag="ACZPAX_09835"
     CDS             1536..1751
                     /locus_tag="ACZPAX_09835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1114236.1"
                     /translation="MAKPNIDISLDGVGESARSAKTKRASIDIPLDGEEPRRPVTDIT
                     MKELEFVNRHHEEYKELRRRAREQGGR"
     gene            1751..>2088
                     /locus_tag="ACZPAX_09840"
     CDS             1751..>2088
                     /locus_tag="ACZPAX_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012425205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase C-terminal domain-containing
                     protein"
                     /protein_id="HIR1114237.1"
                     /translation="MQYILHTPLTKADLAPLKAGDTVLLSGVVYTARDAAHARMMELL
                     DKGEPLPFPIEGAAVYYVGPTPERPGCAIGAAGPTPSGRMDAYTPRLLDLGLACMIGK
                     GKRSEAVKQSV"
BASE COUNT          425 a          672 c          645 g          346 t
ORIGIN      
        1 aagacagtat ttgtggagaa cagagtgcgt cagatgatcc atgccaatct tgaccgcatc
       61 gaggatgtca agatcgttca gaacggcccc ggtgcagcgg gcacggccat cattcatatg
      121 ctgcaggttg cgggggccaa acacattatc gctgtcgatg agcacggcat tctggtcccg
      181 ggccgccctg caggcctcga gggccacaag ggcaaactgg ccgaggagac gaacccggag
      241 aaactctccg gcggtctggc tgaggctatc aaggacgctg atatcttcat tggcacgtcg
      301 attgcaggcg cactgacgcc cgcgctggcc gccacgatgg cgaaagacgc cattgtgttc
      361 gccatggcca accccacgcc agagattatg cctgacctcg ccaagcaggc gggcatccgc
      421 gttatcggca ccggccgttc ggatttcccg aatcaggtca acaatgtgct ggcgttcccc
      481 ggcatcttca agggtgcgct gtccgtccgt gcgcgggata tcaaccccgc catgaagatg
      541 gctgccgcca aggccatcgc gggcctcatc cccgacgatg agctgaacga ggaaaatatt
      601 ctgcccaagg cgtttgaccc ccgcgtggct gacgctgtgg ccgcagccgt tgcccaggct
      661 gcacgggaaa gcggcgtggc gcgcgtatga gcatccgtga aatttccgcc gccgccatca
      721 ccgacgcggt cgaagctctc tgcatcgagg ccaacaccca gctgcccgcc gacgtaaagg
      781 ccgcactgga taaagccgaa gccaacgagc cgtggccgct ggcaaaggaa acgctgggct
      841 tattgcagca aaatttgtgc gttgcagcgg acaaagactt gcccatctgc caggacaccg
      901 gcatggcctg cgtctttatt gagctgggcc aggacgccca catcaacggc gatttgaccg
      961 atgccgtcaa cgagggcgtg cgccgcggct atgaaaaagc atacctgcgc aaatccatca
     1021 ccgctgaccc cttgcagcgc gtcaacaccg gggacaacac ccccgccttc ctgaccgtgc
     1081 atctggtgcc gggcagcggc tgcaaaatca ccgtcgcccc caagggcgcg ggcagcgaaa
     1141 acatgagccg ccttgccatg ctcaagcccg ccgatggggt agagggcgtc aaaaagtttg
     1201 ttttggacac cgtcgagcag gccggggcca acccctgccc acccgtcgtg ctgggcatcg
     1261 gcatcggcgg cagctttgac cactgtgctg cgctggcgaa aaaggcattg ctgcgcccgc
     1321 tcgatacccc caacgccgac ccctactacg cggcgctgga aaaagagctg ctggacgcca
     1381 tcaacgctac cggcttcggg ccgcagggct tcggcggggc cactacctgc ctgggtgtcg
     1441 ccattgagca gaagcccacc catgtggcct gtctgccggt ggcagtcaac atcagctgcc
     1501 atgtgacccg ccgcgccagc cgggaggtgt gagcgatggc gaaaccgaac attgatattt
     1561 cgctggatgg cgtgggcgag agcgcccgct cggccaaaac caaacgcgcc agcattgata
     1621 tccccttgga cggcgaggaa ccccgccgcc cggtgacaga tatcacgatg aaggaactgg
     1681 aatttgtcaa ccgtcaccat gaggaataca aagaattgcg ccgccgtgcg cgggaacagg
     1741 gaggccgctg atgcaataca ttttacatac gccgctgacc aaagcggatc ttgcgccgct
     1801 caaggcaggg gacactgtgc tgctttcggg cgtggtctac accgcccgcg acgccgccca
     1861 cgcccgtatg atggaactgc tggataaggg cgagccgctg ccgttcccca ttgagggcgc
     1921 ggcggtctac tacgtcggcc ccacgccgga gcgcccggga tgtgccatcg gggccgcagg
     1981 gcccacgccc tccggccgca tggatgccta tacgccgcgg ctgctggatc tgggccttgc
     2041 ctgcatgatc ggcaagggta agcgcagcga ggccgtcaag cagtcggt
//
LOCUS       DBMDSQ010000346         2080 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_11758_length_2080_cov_1.736230, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000346 DBMDSQ010000000
VERSION     DBMDSQ010000346.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 2080)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2080)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2080
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11758_length_2080_cov_1.736230"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..970)
                     /locus_tag="ACZPAX_09845"
     CDS             complement(<1..970)
                     /locus_tag="ACZPAX_09845"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007045999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HIR1114238.1"
                     /translation="MDAKIHVSRLSGTATVPPSKSAAHRAVLCAALADGTSHITNIEY
                     SKDIRATLGAAAQLGAKITEEPHSLTIKGHGTAGGFVTVTRPVFCNESGSTLRFMIPL
                     FSLTAQKVRFTGAGRLFDRPQAVYQMLFDRQGLHFEQTPEGITIFGRLRPGGFTLPGD
                     VSSQFISGLLFAAPLMDSESSIEVLPPYESHSYVDLTIDAMQQFGVKVSARARKNGSM
                     MYRVAAPQRYTASDFAVEGDYSQAAFLAVLGCVIGGITITGLNPDSQQGDKVILDILK
                     RCGGKFKEVEGGYHFDRSLLKATKIDLADCPDLGPILFTLGCFCSGE"
     gene            complement(972..1988)
                     /gene="aroF"
                     /locus_tag="ACZPAX_09850"
     CDS             complement(972..1988)
                     /gene="aroF"
                     /locus_tag="ACZPAX_09850"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005943520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HIR1114239.1"
                     /translation="MIISTKKGTPQVELDRIVDNFEKQGLSVTLIRGTDYNVFGLVGD
                     TTKIAEKDVLANPWVENVTRVSAPYKRANRLFHPEDTVVDVNGVKIGGKSPIAVMAGP
                     CSVEGEAHTIKMAKAVKAGGANFLRGGAYKPRTSPYSFQGLGTEGILDLVKAREETGL
                     PIVSELMDEIHIPEFEEYVDVVQIGARNMQNFQLLKAVGKMHKPILLKRGLANTIEEW
                     IMSAEYIMAGGNENVILCERGIRTFERATRNTLDLSAVPVIKEKTHLPIIVDPSHATG
                     DYKYIESMALAAVAAGADGLEIEVHDDPPHAWSDGAQCLKPDKFADVIAKCRKVAAAI
                     GREM"
BASE COUNT          373 a          619 c          645 g          443 t
ORIGIN      
        1 tttcgccact gcaaaagcag cccagcgtga acaggatcgg ccccaggtcc gggcagtcgg
       61 ccaggtcgat cttggtggct ttgagcaggc tgcggtcaaa gtggtagccg ccctcgactt
      121 ccttaaattt gccgccgcag cgctttaaaa tgtccaaaat gaccttatcg ccctgctggc
      181 tgtcggggtt taagccggtg atggtgatgc cgccgatgac gcagcccagc acagccagga
      241 atgccgcctg gctgtaatcg ccctcgactg caaaatcgct ggccgtgtac cgctgcggcg
      301 cggccacgcg gtacatcatg ctgccatttt tgcgggcgcg ggccgacacc ttgacgccga
      361 actgctgcat cgcgtcgatg gtcagatcca cataggaatg gctctcgtag gggggcagga
      421 cctcgatgct gctctcagag tccatcagcg gcgcggcaaa cagcaggccg ctgatgaact
      481 ggctggacac atcgccgggc agggtgaacc cgccggggcg caggcggcca aaaatcgtga
      541 tgccctctgg cgtctgctca aaatgcaggc cttggcggtc aaacagcatt tgatacactg
      601 cctgggggcg gtcaaacagc cgccctgcac cggtaaagcg cactttctgg gccgtcaggc
      661 tgaacagcgg tatcataaac cgcagcgttg acccgctttc gttgcagaac accgggcgcg
      721 tcaccgtcac aaagcccccc gctgtgccgt ggcctttgat ggtcagggag tggggctcct
      781 cggtgatttt ggcacccaac tgggccgctg cgcccaatgt ggcgcggatg tccttactgt
      841 actcgatatt cgtgatatgg cttgtgccgt ccgccaaagc agcgcacaaa actgcacggt
      901 gggccgcact tttgctgggc ggcactgtgg ctgtgccgct caggcggctg acatgaatct
      961 tcgcgtccaa tttacatctc ccggccgatg gcggcggcga ccttgcggca cttggcgatc
     1021 acatcggcaa acttatcggg tttcaggcac tgcgcgccgt cgctccaggc gtggggcggg
     1081 tcgtcatgga cctcaatttc caggccgtcc gcaccggcgg ccactgcagc cagggccatg
     1141 ctctcgatgt acttgtagtc accggtagcg tggctggggt cgacgatgat gggcaggtgg
     1201 gtcttttcct tgatgaccgg cacggcggac aggtccagcg tgttgcgggt cgcgcgctca
     1261 aaggtgcgga tgccgcgctc gcagaggatg acgttctcgt tgccgcccgc catgatgtac
     1321 tcggcagaca tgatccactc ctcgatcgtg tttgccaggc cgcgcttgag cagaatgggc
     1381 ttgtgcatct tgccgacggc cttgagcagc tggaagttct gcatattgcg tgcgccgatc
     1441 tgcacgacgt cgacatactc ctcgaactcg gggatatgga tctcgtccat cagctcactg
     1501 acgatgggca gaccggtctc ctcgcgggcc ttgaccaggt cgaggatgcc ctcggtgccc
     1561 agaccctgga aggagtaggg actggtgcgg ggcttgtacg cgccgccgcg caggaagttt
     1621 gcgccgcccg ccttgacagc ctttgccatc ttgatggtgt gggcctcacc ctcgacgctg
     1681 caggggccgg ccattacggc gatgggggac ttgccgccga tcttgacgcc gttgacgtcc
     1741 acgacggtat cctcgggatg gaacaggcgg ttggcgcgct tgtagggggc agacacacgg
     1801 gtcacgttct caacccaggg gttggccagc acgtcttttt cggcaatttt cgtcgtatca
     1861 ccgacaaggc caaagacgtt gtaatccgtg ccgcggatca aggtgacgga aaggccctgc
     1921 ttctcgaaat tgtcaacgat gcgatccagt tcaacctggg gcgtaccttt tttggtggaa
     1981 ataatcatgg gggaacctct tttttatata ctttttactc tataaaacaa acctttggct
     2041 tccccctcgg gggaaggctt ttacagcgtc tcatccgctt
//
LOCUS       DBMDSQ010000347         2078 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_11772_length_2078_cov_1.706626, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000347 DBMDSQ010000000
VERSION     DBMDSQ010000347.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 2078)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2078)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2078
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11772_length_2078_cov_1.706626"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..889
                     /locus_tag="ACZPAX_09855"
     CDS             <1..889
                     /locus_tag="ACZPAX_09855"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005944436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HIR1114240.1"
                     /translation="QKAQQAQWRNKAISDLYEPGSVFKLITASAALDSGACRATDYFT
                     CAGKISVAGTRFRCANGHIHGTETFARGLAVSCNPCFIQIGARLGKERFCDYFAAFGL
                     REATGIDLPGEIRRSEYYTADKMGPVELASCSFGQSSKVSYLQMLTAVCAVVNGGELM
                     QPYVVAKITAPDGTIIKEMAPTVKRRVISEETSATMRRLMEGVVTGGTGKQAALEGYR
                     VGGKSGTSQKLDSANEGARIASFVSVAPIDDPKIAVLICLDEPHSWTTSGGALSGPVC
                     AEVLRKALPYLGVEPESGG"
     gene            1051..1545
                     /locus_tag="ACZPAX_09860"
     CDS             1051..1545
                     /locus_tag="ACZPAX_09860"
                     /inference="COORDINATES: protein motif:HMM:NF024212.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIR1114241.1"
                     /translation="MHEPSPNDCGCCADVHFGALLNQSSRVIRRALDARISGVNPDLS
                     GVRGMVLGDIVRANRNGRDVYQRDIEQWFNIRRSSVTALLQGMEQDGFITRCAVEKDA
                     RLKRLVATDKGRACHAEIEASIAQFESDLQKGIDPQQAAVARAVLEQTLRNAQHILEE
                     GNTN"
     gene            1563..>2078
                     /locus_tag="ACZPAX_09865"
     CDS             1563..>2078
                     /locus_tag="ACZPAX_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008121034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HIR1114242.1"
                     /translation="MLKTLGRETKGIRLVSVLTPLFMIAEVIMEMIIPKLMASIIDNG
                     VTPGNMQVIYTVGAQMIVAALFGLLFGILGAVSGSHAATGFARNLRRAMFENIQTFSF
                     ANIDKYSTAGLVTRMTTDVTNIQNAYQMLLRMSIRAPASMIVALIMSYTINRQLANIY
                     LIAVILLGCALV"
BASE COUNT          466 a          603 c          618 g          391 t
ORIGIN      
        1 gcaaaaagct cagcaggccc agtggcgcaa caaggccatc agcgacctgt acgagccggg
       61 cagcgtgttc aagctcatca cggcgtcggc ggcgctggac tccggcgcgt gcagggccac
      121 agattatttt acctgcgcgg gtaaaatttc tgtcgcaggt acgcggttcc gctgcgccaa
      181 tgggcacatc cacggcaccg agacatttgc ccgggggctg gcggtcagct gcaacccctg
      241 ctttatccaa atcggcgcac gtctcgggaa agaacgtttc tgcgattatt ttgcggcctt
      301 cggcctgcgg gaggcaacgg gtatcgacct gccgggcgag atacgccgca gcgagtatta
      361 cacggcggat aaaatgggcc cggtggagct ggcgagttgt tcctttgggc agagcagcaa
      421 ggtcagctat ttacagatgc tcacagcggt ttgtgcggtg gtgaacggcg gcgagctgat
      481 gcagccctat gtggtggcga aaatcacggc cccggacggg actattataa aggaaatggc
      541 gccgaccgtg aagcggcggg tcatcagcga ggaaacctcg gccacgatgc gccgcttgat
      601 ggagggcgtc gtgaccggcg gcaccggcaa acaggccgcg ctggaaggct accgtgtcgg
      661 cggcaagagc ggtacgagcc agaagctgga cagcgccaac gagggggcgc gcattgcaag
      721 ctttgtgtcg gtcgcgccca tagacgaccc gaaaattgcg gtgctgatct gcttggatga
      781 accccacagc tggaccacca gcggtggggc actctccggc ccggtgtgcg ctgaggtact
      841 aagaaaggcg ctgccgtatt taggggtgga gccggaaagc ggcggctgat ggcaaacgcg
      901 cacgcaaagc cccgcagaca caacctaaaa acctcttgac attccccaaa cctgtgatat
      961 actagtaaag tacgtaaacg aacagttaga aagcaaacaa tccacaatct aactgattat
     1021 cgcaaaaacc aacagggaag ggaggaaatc atgcacgaac catcgccaaa tgactgcggc
     1081 tgctgtgccg atgttcactt cggcgcactg ctgaaccaaa gctcgcgggt catacgcagg
     1141 gcgctcgatg cccgcatttc cggcgtcaac ccggatcttt ccggtgtgcg gggcatggtg
     1201 cttggcgaca tcgtccgcgc caaccgcaac gggcgggatg tctaccagcg cgatattgaa
     1261 cagtggttca acattcgccg ttccagcgtc accgcccttt tgcagggcat ggagcaggac
     1321 ggtttcatca cccgttgcgc cgtcgagaag gacgcccgct tgaaacggct tgtcgccacc
     1381 gacaagggcc gcgcctgcca tgcggaaatt gaagccagca tcgcacagtt tgaaagtgac
     1441 ctgcagaagg gcattgaccc gcagcaggcc gccgtcgccc gcgctgtgct ggaacaaacc
     1501 ctgcgcaacg cgcagcacat cctagaagag ggaaatacca actaagagag gggaaatatc
     1561 acttgttaaa aacactcgga cgtgaaacca agggcattcg gctcgtctct gtgttgacgc
     1621 cgctgtttat gattgcggaa gtcatcatgg aaatgatcat cccgaagctc atggcctcca
     1681 tcattgacaa cggcgtcacg ccgggcaata tgcaggtcat ctacaccgtc ggcgctcaga
     1741 tgatcgtggc tgcgctgttc ggcctgttgt tcggcatcct gggtgcggtc agcggttccc
     1801 atgcggccac cggctttgcc cgcaacctgc gccgtgcgat gtttgaaaac atccagactt
     1861 tcagctttgc caacattgac aaatactcca cggcgggcct tgtcacccgt atgaccactg
     1921 atgtgacgaa cattcagaat gcctaccaga tgctgctgcg tatgtccatc cgcgcacccg
     1981 ccagcatgat cgtggcgctc atcatgtcct acaccatcaa ccgccagctg gccaacattt
     2041 acttgatcgc cgtcattctg ctgggatgcg cactggtg
//
LOCUS       DBMDSQ010000348         2069 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_11821_length_2069_cov_1.937500, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000348 DBMDSQ010000000
VERSION     DBMDSQ010000348.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2069)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11821_length_2069_cov_1.937500"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(336..1553)
                     /locus_tag="ACZPAX_09870"
     CDS             complement(336..1553)
                     /locus_tag="ACZPAX_09870"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014120145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HIR1114243.1"
                     /translation="MARFTLPRDLYHGPNALEALKTLPGKRAMICVGGGSMKRFGFLD
                     RAEAYLKEAGMEVELFEGIEPDPSVETVMKGAAAMEKFQPDWIVAIGGGSPIDAAKAM
                     WIKYEYPDITFEDMCKVFGIPPLRRKAHFCAISSTSGTATEVTAFSIITDYEKGIKYP
                     IADFEITPDVAIVDPELAHTMPKKLVAHTGMDAMTHAIEAYVSTANCDFTDPLALHAI
                     EMIQADLVGSYNGDMEKRDAMHNAQCLAGMAFSNALLGIVHSMAHKTGAAFADYGAHI
                     IHGAANAMYLPKVIAFNAKDPTAKARYGVIADKMHLGGANDDEKVDLLIKYLRGMNDD
                     LNIPHCIGHYGPDSYPAEQGFVPENVFLERLPEIAKNAIADACTGSNPRQPSQEEMEK
                     LLKCCYYDTEVDF"
     gene            1749..>2069
                     /locus_tag="ACZPAX_09875"
     CDS             1749..>2069
                     /locus_tag="ACZPAX_09875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1114244.1"
                     /translation="MRHITPQQNAKMRLRTRLLAAVLAVGCLGGLVVRLFILMLRDPG
                     GYAARAADQQLRGATLPAARGEIYSADGTLLAASKTCWTIRAAPREMADDKVEPAARA
                     LSEIL"
BASE COUNT          432 a          542 c          631 g          464 t
ORIGIN      
        1 ggggccagat cagggtaaaa agcgcacgtt tagtgaagag cggcgtttgc ggctttgcag
       61 tggctgccat gaaattactc ctttttttct tggattttac cgttattata ccgcaaaatt
      121 tatacgaaat caacgttttg acacttgtgc aaaatatagc gtaaaacatt gtaacggaag
      181 cgtttgcagc caaaataacg aaaaatcaaa tcgcaaggat attgcctatt ttacaaaaaa
      241 gcaaggcccc ggccgcagag ggacgcggcc ggggcagggg aacacagcag cttggaggtt
      301 actgcgggct tgttaaagtt aggaataaat ctggtttaga agtcgacctc ggtgtcgtag
      361 tagcagcact tcagcagctt ttccatctct tcctgactgg gctggcgcgg gttggaaccg
      421 gtgcaggcgt cggcaatcgc gttcttggcg atttcgggca gtctctcaag gaagacattc
      481 tccggcacga agccctgctc tgcggggtag gagtcggggc cgtagtgacc gatgcagtgc
      541 gggatgttca aatcgtcgtt catgccgcgc aggtatttga tgagcagatc gaccttctcg
      601 tcgtcgttcg cgccgcccag gtgcatcttg tcagcgataa cgccatagcg tgccttggcg
      661 gtcggatcct tcgcattgaa ggcaatgacc ttgggcaggt acatggcgtt ggctgcgccg
      721 tggatgatgt gggcaccgta atcagcaaag gccgcaccgg tcttgtgggc catgctgtgg
      781 acgatgccca gcaaagcgtt cgagaacgcc ataccggcca ggcactgggc gttgtgcatc
      841 gcatcgcgct tttccatatc gccgttgtag ctgccgacga gatccgcctg aatcatctcg
      901 atggcgtgca gggccagcgg gtcggtgaag tcgcagttgg cggtggaaac ataagcctcg
      961 atggcgtggg tcatggcgtc cataccggtg tgggcgacga gtttcttagg catggtgtgg
     1021 gccagctctg ggtcaacgat ggcgacatcg ggggtgatct caaagtcggc gatggggtac
     1081 ttgataccct tctcgtagtc ggtgatgatg gagaacgccg tgacctcggt cgcggtgccg
     1141 gaggtggaag agatggcgca gaaatgggcc ttgcggcgca gcggcgggat gccgaacacc
     1201 ttgcacatat cctcaaaggt gatatcgggg tactcgtact tgatccacat ggccttggcg
     1261 gcatcgatgg gggagccgcc gccgattgcg acgatccagt cgggctggaa tttttccata
     1321 gcagcagcgc ccttcatgac tgtctcgacc gacgggtcag gctcaatgcc ttcaaacagc
     1381 tcaacctcca tgccggcctc ttttaagtac gcctccgcac ggtccaggaa gccgaaacgc
     1441 ttcatcgagc cgccgccgac gcaaatcatc gcgcgtttgc cgggcagcgt tttcagtgct
     1501 tccagcgcgt tggggccgtg gtacaggtca cgaggtaaag taaatctagc catagggtta
     1561 tgcctcctta tataaataaa atgctccttt ttgactttga taattaaatg tcaaaatcta
     1621 ttatatacag tatacaccct ttatgtctaa atggtcaatc gacaatacgg agaatttact
     1681 gccgataatt cgtcattttg cccattgcgg cctcatacgc ttattgtatg gggaaggggg
     1741 cagagtgtat gcgtcatatt acgccgcagc aaaacgcaaa aatgcgcctg cgcacgcggc
     1801 ttttggcggc ggtgctggcg gtggggtgcc tgggcgggct ggtcgtgcgg ctgttcatcc
     1861 tgatgctgcg tgaccccggc ggctacgcgg cccgtgccgc cgaccaacag ctgcgcgggg
     1921 ccacgctgcc cgccgcccgc ggggagattt actccgccga cggcacgctg ctggctgcca
     1981 gcaagacctg ctggaccatc cgcgccgccc cgcgggaaat ggccgacgac aaagttgaac
     2041 ccgctgcccg ggcgctgagc gagatcttg
//
LOCUS       DBMDSQ010000349         2069 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_11825_length_2069_cov_1.060976, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000349 DBMDSQ010000000
VERSION     DBMDSQ010000349.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2069)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11825_length_2069_cov_1.060976"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            146..1399
                     /locus_tag="ACZPAX_09880"
     CDS             146..1399
                     /locus_tag="ACZPAX_09880"
                     /inference="COORDINATES: protein motif:HMM:NF019310.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR1114245.1"
                     /translation="MKQTLWTRDFTRITAATALGAAGGIISQFALSFLVFDETGSTLA
                     AALSVAIQLIPMVLLPLIIAPMMDRLPRKPFLVWGDLLNGVCYAGAGIFLLHNAFSYT
                     AYLAFSLLVSCLGAFDELAYNSFYPLLLPEGQEERAYTVSAMLYPILKVLMMPLAALL
                     YDTLGVGRLLLIQAVLSVLAALIENSIHVQENRRDQEPLLSLKTWWRDVREAADYLRQ
                     ERGLHGLYTYMAVTNGMAMGYAPLLIAFFRTAQGFTTAMYSFFSVAEFTGRTIGGVVR
                     YRFSLPERKRFGFLFGVYQTYELMDACLLWLPYPLMLVNRALCGFLGIQSATIRQAAV
                     QRYIPDRLRARLNAYESLLCTAAGAVLSLAIGALGEVLDYRLCMTICGLTTMLVCWLT
                     VFLRRRQVQQVLASRPNAAQPGVPQ"
     assembly_gap    1710..1719
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1804..>2069)
                     /locus_tag="ACZPAX_09885"
     CDS             complement(1804..>2069)
                     /locus_tag="ACZPAX_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014118902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HIR1114246.1"
                     /translation="SYRKIGDAVGLSPNTIRKHIRSLEERDLLVTEPTQVTTKDGHKR
                     NGNLLFTIRPIQFALQQDYDRQMRKLDEDAARRKYAGFIENTG"
BASE COUNT          342 a          630 c          586 g          501 t
ORIGIN      
        1 caggcgtgcc gcctgctgga ggagtttcct gcaaaatagc agttccgtgc atctaagcgg
       61 gtcgtcacgg gaatattttt cccatgatga cccacttttc ttttgtaagc ggctatgcga
      121 taatcatagc agtgaggtgg atgtcatgaa acaaacgctc tggacgcggg actttacccg
      181 catcactgcc gcgacggcac ttggcgctgc cggcggcatt atcagtcagt ttgccctgtc
      241 ctttctggtc tttgacgaga cgggcagcac actggccgca gcactgtcgg tggccattca
      301 gctgattccc atggtgctgc tgccgctgat catcgccccc atgatggacc gcctgccgcg
      361 caaaccgttt ctggtatggg gcgatctgct caacggcgta tgctacgccg gagcggggat
      421 cttcctgctg cacaacgcct tctcctacac cgcctatctg gccttttctc tgctggtgtc
      481 gtgtctcggc gcttttgacg agctggccta caacagcttt taccctctgt tgctgccgga
      541 ggggcaggag gagcgggcat acaccgtttc tgccatgctc taccctattt tgaaggtcct
      601 gatgatgcca ttggcggcac tgctgtatga tactctgggc gtggggcggc tgctgctgat
      661 ccaggctgta ctgtcggtgc tggccgccct cattgaaaac agcatccatg tgcaggagaa
      721 ccgccgggat caggagccgt tgctgtctct gaaaacctgg tggcgggacg tccgggaagc
      781 cgccgattat ctgcggcagg aacgcggcct gcacggtctg tatacgtaca tggccgtcac
      841 caacggtatg gctatgggct acgctcccct gctcattgct ttcttccgca cggcgcaggg
      901 cttcaccacc gccatgtatt cctttttctc tgtggcggag ttcaccgggc gtaccatcgg
      961 cggagttgtc cgctaccgct tttccctgcc ggagcgaaag cggttcggct tcctgttcgg
     1021 ggtatatcag acctatgagc tgatggacgc ctgcctcctg tggctgccgt accccttgat
     1081 gctggtaaac cgggctctct gcggctttct ggggatccag agcgccacca tccgtcaagc
     1141 agcagtacag cgctatatcc cagaccggct gcgtgcccgg ctcaacgcct atgaatcctt
     1201 gctctgcacg gcggcgggcg cggtgctgtc gctggccatc ggcgcgctgg gggaggtcct
     1261 ggactaccgg ctctgcatga ccatctgcgg tctgaccact atgctcgtgt gctggctaac
     1321 agtgttcctg cgtcggcgtc aggtgcagca ggtgctggcc agccgcccaa acgcggccca
     1381 acccggcgtc ccgcaataac gcgctatctc tgcgcactat ttccgctgaa ccccgtcgtt
     1441 ttccttgaaa cggcgggctt ttttcaaatc aagaccccac aggtgctatt tttacccgct
     1501 caacaactcc ttcgtaaatc cccgaaactg cccgcactgc gggcagaagt ggacggtcag
     1561 acgcagaaaa gcgtcaggcc tttctcctgc ttcgccgctt gcggcttttc cctctgccga
     1621 aagccgcctg caggcgtcct tcttctgcgt ttgccatttc cgcaacgctc ccgcacggcg
     1681 gtgcaacgtc cggtcttcgg gcttccggcn nnnnnnnnng gtacacgctc ggctgggcgg
     1741 cgaaagcggc acaaaccgcc aacacgggcg cgacaggctg ggattggcgt tttggctgtg
     1801 cgttcagcct gtgttttcaa tgaaaccggc atatttcctc cttgcggcat cctcgtccag
     1861 tttccgcatc tgccgatcat agtcctgttg gagcgcgaat tgaattggtc ggattgtgaa
     1921 tagcagattg ccatttcgct tgtgaccatc cttcgttgta acttgcgtcg gctctgtgac
     1981 gagcaggtcg cgttcttcga gtgagcggat atgcttacga atcgtgttcg gactcaaccc
     2041 aaccgcatca ccgatttttc ggtagcttg
//
LOCUS       DBMDSQ010000350         2064 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_11857_length_2064_cov_1.747326, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000350 DBMDSQ010000000
VERSION     DBMDSQ010000350.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 2064)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2064)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2064
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11857_length_2064_cov_1.747326"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..354
                     /locus_tag="ACZPAX_09890"
     CDS             <1..354
                     /locus_tag="ACZPAX_09890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047160.1"
                     /note="back-translocating Elongation Factor EF4; binds to
                     the ribosome on the universally-conserved alpha-sarcin
                     loop; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HIR1114247.1"
                     /translation="RLDFLLNGEVVDALSMIVFADNAYAKGRRICEKLKENIPRALFE
                     IPVQAAVGGKVIARETVKAMRKDVLAKCYGGDITRKKKLLEKQKEGKKKMRQLGSVTL
                     PSEAFTAVLKLDSDS"
     gene            complement(632..1780)
                     /gene="fucO"
                     /locus_tag="ACZPAX_09895"
     CDS             complement(632..1780)
                     /gene="fucO"
                     /locus_tag="ACZPAX_09895"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003477482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HIR1114248.1"
                     /translation="MADRIVLNSISYHGHGAIENIVPELTARGYKKAFVCSDPDLVKF
                     GVTKKVTDLLEAAGFAYALYSEIKPNPTIKNVQDGVAAFQAAEADCIVTIGGGSSMDT
                     AKAIGIIINNPEFADVRSLEGVAPTKKHAVFTIAVPTTAGTAAEVTINYVITDVEKKR
                     KFVCVDTNDIPEIAVVDPDMMSSMPKGLTAATGMDALTHAIEGYITKGHCTISDMFHL
                     EAIKLISENLRGAVQNTPEGREGMALGQYIAGMGFSNVGLGIVHSMAHGLSALYDTPH
                     GVACAIILPTGLEYNKTVAGERYRAVGKAMGVKGIDEMNDAEAADATIAEVKKLSADV
                     GIPADLKGILKEEDVQFLAESAFADACCPGNPRDTNVEEIKALYRSLM"
BASE COUNT          432 a          546 c          619 g          467 t
ORIGIN      
        1 cgtctggact ttttgctcaa tggcgaggtc gtggatgccc tgtcgatgat cgtgtttgcg
       61 gacaacgcct acgccaaggg ccgccgcatc tgcgaaaaat tgaaggagaa cattccccgc
      121 gcactgtttg aaattcctgt gcaggcggcc gtgggcggca aggtcattgc ccgcgagaca
      181 gtcaaggcca tgcgcaagga cgtgttggcc aagtgctacg gcggcgacat cacccgcaaa
      241 aagaagctgc tggaaaagca gaaggaaggc aaaaagaaaa tgcgccagct gggcagcgtg
      301 accctgccga gcgaagcgtt tacagcggtg ctgaaactgg attcggattc gtaacggggg
      361 ttttgttcct tctttgcaaa gaaggaaccg aagaaattcc aaacaaaata ttcgtagggg
      421 agggatttat ccctccccta cttattatcc cgccgcgccc tgcaaatttt gccactctgt
      481 aggggccgca catgtgcggc ccgcagcttt tcggcaaaat tgattttgcg ggaaggccgc
      541 gggccgggca tgcccggccc ctacaatgcc gcaaaataaa atcgggtatc catccaaacg
      601 atggataccc gattttgttg ttatgcggta attacatcag gctgcggtac agtgctttga
      661 tttcctcgac gttcgtgtcg cgtgggttgc cggggcaaca ggcatcggca aaggcggact
      721 cggccaggaa ctggacatcc tcttccttca gaataccctt caggtcagcg ggaatgccga
      781 cgtcagcaga gagcttcttg acctcggcga tcgttgcatc ggcggcctcg gcgtcgttca
      841 tctcgtcgat gcccttcacg cccatggcct tgccgacggc gcggtaacgc tctcctgcaa
      901 cggttttgtt gtattccagg ccggtgggca ggatgatggc gcaggcaacg ccgtgggggg
      961 tgtcatacag ggcagacaga ccgtgggcca tgctgtgcac gatgcccaga ccaacgttgg
     1021 agaagcccat accggcaatg tactggccca gggccatgcc ctcgcggccc tccggggtgt
     1081 tctgcacagc gccgcgcagg ttttcggaga tcagcttgat ggcctcgagg tggaacatat
     1141 cggagatggt gcagtgaccc ttggtgatgt agccctcaat cgcgtgggtc agggcgtcca
     1201 tgccggtggc agcggtcagg cccttgggca tactggacat catatcaggg tcaaccacgg
     1261 cgatctcggg gatatcgttg gtgtccacgc agacgaactt gcgcttcttc tccacgtcgg
     1321 tgatgacgta gttgatggtg acttcggccg cagtgcctgc ggtggtgggc acagcaatag
     1381 taaagacggc gtgcttcttg gtgggggcaa cgccctccag gctgcggacg tcggcaaact
     1441 cagggttatt gatgatgatg ccgatagcct tggcggtgtc catggaagaa ccgccgccga
     1501 tggtcacgat gcagtcagcc tcggcagcct ggaaagccgc cacgccgtcc tgcacgttct
     1561 tgatggtagg gttgggcttg atctcgctgt acagggcata ggcaaaaccg gcagcctcca
     1621 gcaggtcggt gactttcttg gtgacgccga acttgaccag atcagggtca gagcagacga
     1681 aagccttttt gtagccgcgg gcggtcagtt cgggaacgat gttctcgata gcgccgtggc
     1741 cgtggtaaga gatagagttc aaaacaatac gatctgccat agaatattct ctcctttaaa
     1801 catatcggct ttgggccgat gatttccata ttctacggtt attataccac cgccattttc
     1861 acagtacaat ccaaaaaagt ggcgcaaagt ttgtgaaaag tgtacaaggc ctgtccttag
     1921 gccctgctgt accggttcag cgtcatcgaa atcgtcaggt tgattttggt gacctgcatc
     1981 atgtggcggg aaaatgccgg cagcgtgctg tcttttttcg tgccgcccca ttccagcaat
     2041 tcttccggtt ttttcatggg tttg
//
LOCUS       DBMDSQ010000351         2054 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_11931_length_2054_cov_1.814132, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000351 DBMDSQ010000000
VERSION     DBMDSQ010000351.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 2054)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2054)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2054
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11931_length_2054_cov_1.814132"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            331..627
                     /locus_tag="ACZPAX_09900"
     CDS             331..627
                     /locus_tag="ACZPAX_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046192.1"
                     /note="This anti-anti-sigma factor, or anti-sigma factor
                     antagonist, belongs to a family that includes
                     characterized members SpoIIAA, RsbV, RsfA, and RsfB;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor antagonist"
                     /protein_id="HIR1114249.1"
                     /translation="MAKVTFIPGSGTLAAYLSGEIDHHAAQSLRKEIDAQIDDRLPEL
                     LTLDFSGVTFMDSSGVGLILGRGRHIGALGGRLTVQNPPRAVRRMLDLAHITYA"
     gene            644..1078
                     /gene="spoIIAB"
                     /locus_tag="ACZPAX_09905"
     CDS             644..1078
                     /gene="spoIIAB"
                     /locus_tag="ACZPAX_09905"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015565629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA];
                     GO_process: GO:0042174 - negative regulation of
                     sporulation resulting in formation of a cellular spore
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor"
                     /protein_id="HIR1114250.1"
                     /translation="MKMLNQVKITFAGRSVNEGFARAALAAFLVQLDPTVPQLADIKT
                     AVSEAVTNCIVHAYPDRIGPITMTAAIYEGGVVRITITDRGVGIPDIAKAMEPMFTTG
                     DAEERAGLGFAVMQSFMDKVKVSSAPGRGTRVTLTKRLDARA"
     gene            1111..1794
                     /locus_tag="ACZPAX_09910"
     CDS             1111..1794
                     /locus_tag="ACZPAX_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR1114251.1"
                     /translation="MPADIRDLPREEFIEKNLGLVHACAGRFKGRGIEYEELYAAGCL
                     GLVKACDGFDTGRGVCFSTYAVPVILGEIKKLFRDGGTVKVSRSLKELSLKINAERER
                     CLKRTGQEPSVTELAETLDTTPEQIALAIRAALPTLSLTPTDGEDGLKEFDVPVDSPE
                     EELSDRIGLAEVMDKLDTNDRQLIRLRFFAGRTQSETARVLGTTQVQISRRERRILKW
                     MREQLLDAL"
BASE COUNT          441 a          675 c          550 g          388 t
ORIGIN      
        1 agcagaaaga taacccgcgt cccgcgattt tgacaaaata aacaccccca agcccgtata
       61 ataacggtac gggctttttt tatttgccgc gattggcttt acatcagctt ttcaatcgct
      121 tgcaggggag ggattcatcc ctcccgcgca ccttgccttt atcgcgcaat taaaatttca
      181 ccacccaggt aggggccggg cacgcccggc cctccacgtt cccattatca ccctatcaaa
      241 atacaaacca ctccgtaggg gcggggctct gctccgcccg cacaacgtcc ccgaaaccgc
      301 aactttacct acaaaaagaa ggacaaacct atggccaaag taaccttcat ccccggcagc
      361 ggtaccctcg ccgcctacct cagcggcgag attgaccacc acgctgccca atcgctgcgc
      421 aaggaaatcg acgcccaaat cgatgaccgc cttcccgaac ttctgacgct ggacttctcc
      481 ggcgtcactt tcatggattc ctccggcgtc gggcttatcc tgggccgcgg ccgccacatc
      541 ggcgcactgg gcggccgcct gactgtccag aacccacccc gggctgtgcg gcgcatgctg
      601 gacctggccc acatcaccta cgcctgaacg gaggaaaaca cagatgaaaa tgctcaatca
      661 ggtaaaaatc acctttgcgg gccgctccgt caacgagggc tttgcccgcg cggcgctggc
      721 ggcgtttctg gtgcagctgg accccaccgt gccccagttg gctgatatca agaccgctgt
      781 gtccgaggcc gtgaccaact gcattgtcca cgcctacccg gaccgcatcg gccccatcac
      841 aatgacggca gccatctatg agggcggcgt cgtgcgtatc accatcaccg accgtggcgt
      901 cggcatcccg gatatcgcta aagccatgga accgatgttc accacaggcg acgccgagga
      961 acgcgcgggc ctgggctttg cggtcatgca gagctttatg gataaggtca aagtttcctc
     1021 tgcgccgggg cgcggaaccc gcgtgaccct gaccaagcgg ctggatgccc gcgcgtgaac
     1081 cccctacata tataaaagga ggggacagcc atgcctgctg acatccgaga tctgccgcgc
     1141 gaagaattta ttgagaagaa tttgggcctg gtccatgcct gcgccgggcg cttcaagggg
     1201 cgggggatcg agtacgagga gctgtatgcc gcgggctgcc tggggctggt caaggcctgc
     1261 gatggttttg atacagggcg gggtgtctgc ttttccacct atgccgtgcc ggtgattttg
     1321 ggagaaatca aaaaactgtt ccgcgatggc ggcacggtca aggtgagccg ttctttaaaa
     1381 gaattgagct tgaagatcaa cgccgagcgc gagcgctgcc tgaaacgtac cggccaggag
     1441 ccgagtgtca cagaacttgc agagacactg gacacaacac ccgagcagat tgcccttgcc
     1501 atccgtgcag ccctgcctac cttaagcctg acaccgaccg atggcgagga tggactgaaa
     1561 gagtttgacg tgccggtgga ttcgccggag gaagaactct ccgaccgcat cggcctggcc
     1621 gaggtgatgg acaagctgga caccaacgac cgccagctca tccgcctgcg ctttttcgct
     1681 ggccgtaccc aaagtgagac tgcccgcgtc ctgggcacca cccaagtcca aatctcccgc
     1741 cgcgagcgcc gcatcctaaa atggatgcgc gaacaactgc tggatgcact gtgaaataca
     1801 gctgtgtccc aacccacttc gccaacctgc ccagccgcca ctcggtaggg gccgggcatg
     1861 cccggacctc cgccttgccg ttactgcaat ttaatcttcc atattgtagg gaggggactt
     1921 gaccctccgc cgcacattgc ctttattgac ccttactccc gccaggtcgc ccatgtaggg
     1981 gcggcataca tgccgcccgc cccaccttcc catcacaacc ccttgcgcct atcaccaccc
     2041 accaccccaa tata
//
LOCUS       DBMDSQ010000352         2050 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_11959_length_2050_cov_1.815803, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000352 DBMDSQ010000000
VERSION     DBMDSQ010000352.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2050)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11959_length_2050_cov_1.815803"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            65..1630
                     /locus_tag="ACZPAX_09915"
     CDS             65..1630
                     /locus_tag="ACZPAX_09915"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009326074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0005998 - xylulose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HIR1114252.1"
                     /translation="MLTLRNCTLGIEFGSTRIKAVLVDDACKPVAQGDFTWENRLENG
                     VWTYPLDEVWRGVQAAYAALAADVQARYGQPLTTVGCIGISAMMHGYLPFDAAGNLLV
                     PFRTWRNTMTGQAAEALTAAFGFNIPQRWSVAHLYQAMLNREPHLAQLDFFTTLAGYV
                     HWQLTGRKVLGVGDASGMFPIDSATGGYNAAMLEKFNALAAKQGYPVDLASLLPTVLP
                     AGEDAGTLTAEGAKLLDPTGVLQPGVPFCPPEGDAGTGMAATNSVAPRTGNVSAGTSI
                     FAMVVLEKPLSKVYPEIDMVTTPDGAAVAMVHCNNCTSDLNAWVDLLAESAALCGAGI
                     DKGALFTRLFNESLKGAPDCGGVVPINYISGESVTHFDAGLPLLLRRPNSDFTLANFM
                     RANIYSAFATLALGLNILKQEHVALDTLLGHGGIFKTPGVAQRYLAAAAGAPVTCMET
                     AGEGGPYGMALLAAYRLYRRAGETLAAYLNRCVFADARSVTLAPDADDTDGFAKFLIE
                     YKNALNAERAVVS"
     gene            1776..>2050
                     /locus_tag="ACZPAX_09920"
     CDS             1776..>2050
                     /locus_tag="ACZPAX_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014117074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HIR1114253.1"
                     /translation="MLHTYEFWSVVGSQFLYGPEVLETVAARAAEMADKLNASGNLPC
                     KIVYKVTAKTNAEISDVVREANYDKHCAGIITWCHTFSPSKMWINGFA"
BASE COUNT          394 a          618 c          651 g          387 t
ORIGIN      
        1 tgaatgcaca tacggacata gccatgataa aaaacaaagc tccacttgtc aaggaggaaa
       61 atccatgctt accttacgaa attgtaccct cggcattgag tttggctcca cccgcattaa
      121 agcggtgctg gtggacgatg cctgcaagcc ggtcgctcag ggtgacttta cctgggagaa
      181 tcggctggaa aacggtgtct ggacctaccc gctggacgaa gtctggcgtg gtgtgcaggc
      241 cgcctacgcc gccctggccg ccgatgtgca ggcccggtac ggccagccgc tgaccacggt
      301 gggctgcatc ggcatttcgg ccatgatgca cggctatctg ccttttgacg cggcgggcaa
      361 tctgctcgtg ccgttccgca cctggcgcaa cacgatgacc ggccaagcgg cggaagcgct
      421 gaccgcggcg ttcgggttca acatccccca gcggtggagc gtagcgcacc tgtaccaggc
      481 catgctgaac cgcgagccgc acctggcgca gctggatttc ttcacaacac tggccgggta
      541 tgtacactgg cagctgaccg ggcgcaaggt gctgggcgtg ggcgacgcca gcggtatgtt
      601 cccgattgac agcgccaccg gcgggtataa cgctgccatg ctggaaaaat tcaacgcgtt
      661 ggcggcaaag cagggctatc ctgtagacct tgccagtctg ctgcccaccg ttttgcccgc
      721 aggtgaggac gcgggcacgc tgaccgccga gggcgcgaag ctgctggacc ccaccggcgt
      781 tttgcagccc ggcgtgccgt tctgcccacc cgaaggcgac gcaggcaccg gcatggccgc
      841 cacgaacagt gtggccccgc gcaccggaaa tgtcagcgca ggcacgagta tttttgccat
      901 ggttgtgctg gaaaagccgc tttccaaggt ctacccggaa atcgacatgg tcacgacccc
      961 cgacggtgcg gcggtggcca tggtccactg caacaactgc accagcgacc taaacgcctg
     1021 ggtcgatctg ctggccgaga gcgcggcgct ctgcggggct ggcatcgaca agggcgcgtt
     1081 gttcacccgg ctgttcaacg agtctttgaa gggtgcgcct gactgcggcg gcgtggtgcc
     1141 catcaactat atctccggtg agagcgtgac ccactttgat gcgggcctgc cgctgctgct
     1201 gcgccgccca aacagtgatt ttacgctggc gaactttatg cgggccaata tctattcggc
     1261 ctttgccacg ctggcgctgg ggctaaacat tttgaagcag gagcacgtgg cgttggatac
     1321 gctgctgggc cacggcggca ttttcaaaac gccgggtgtg gcgcagcggt atctggccgc
     1381 tgcggcaggt gcgcccgtca cctgcatgga gaccgcgggc gagggcggcc cttacggcat
     1441 ggcgctgttg gcggcctacc gcctgtaccg cagggcaggg gagacgctgg cggcgtatct
     1501 gaaccgctgc gtgttcgctg atgccagaag cgtaacgctg gccccggatg ccgacgatac
     1561 ggatgggttc gcgaagttcc tgatcgaata taaaaatgcg ctgaacgccg agcgggcggt
     1621 ggtgtcctga aaatttctcc cgccgtaggg gccgggcatg cccggcccgg tcgtgtaagg
     1681 caagtgtgcg cttccgggaa agttgcgggc cggacatatc cggcccctac cgagtactgc
     1741 aggcactggt tatttaaaag aaggaggaaa cttttatgtt gcatacctat gaattttggt
     1801 ccgtcgtggg cagtcagttt ttgtacggcc ctgaagtcct cgagaccgtc gccgcccgcg
     1861 cggcggagat ggccgataag ctgaacgcct ccggcaatct gccctgcaaa atcgtctaca
     1921 aagttaccgc caagaccaac gccgaaatct ccgacgtggt gcgcgaggcc aactacgaca
     1981 agcactgcgc gggcatcatc acctggtgcc acaccttcag ccccagcaaa atgtggatca
     2041 acggctttgc
//
LOCUS       DBMDSQ010000353         2021 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_12180_length_2021_cov_1.763542, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000353 DBMDSQ010000000
VERSION     DBMDSQ010000353.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 2021)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2021)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2021
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12180_length_2021_cov_1.763542"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1590
                     /locus_tag="ACZPAX_09925"
     CDS             <1..1590
                     /locus_tag="ACZPAX_09925"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015565595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIABC"
                     /protein_id="HIR1114254.1"
                     /translation="IDMLARMMQMLMNDDFVEKLRAAKTPAEFLDAIDAQEDAQFGEE
                     SFTQQEIPQSGYRVLAVTACPNGIAHTYMAAEALTKMGDKLGLPTKVETNGSDGAKNV
                     LTPEEIAACDGIIIAADKNVETARFDGKPVIFARVDDGIHKPEELIKKIAHGEAPIYR
                     AEAGAKAQQSAGGASDSLGHSLYKHLMNGVSHMLPFVVGGGIMIALAFLLDDYTIDPS
                     NFGMNTPVAAFFKTVGNAAFGYMLPILAGFIAMSIADRPGLAVGFAGGVLAMNGTNFA
                     GLASGDTTGISGGFLAALLAGFAAGYIVQFLKKITEKLPVSLNGIRPMLIYPLGGILV
                     TGLVMCGINPVMGIINTAMTDWLNAMGDTSKVMLGAIVAGMMSIDMGGPFNKAAYVFG
                     TAALAAGNYEVMAAVMVGGMVPPIAIALSTTFCPKKWSPDERRNGIVNYVMGLCFVSE
                     GAIPYAAADPLRVLPSCIIGSALAGSLSMVFGCALRAPHGGIFVFPVVDHALLYVIAL
                     AVGSAVGALILSLLKKEYVKE"
     gene            complement(1756..>2021)
                     /locus_tag="ACZPAX_09930"
     CDS             complement(1756..>2021)
                     /locus_tag="ACZPAX_09930"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005924931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HIR1114255.1"
                     /translation="TGAETRICVPGHMQRGGSPSAYDRVLATQFGSYAAGLVADEHYG
                     VTVAMVNRHVTANPLADIAGKTRGVPGDCDMLNVARAMGISLG"
BASE COUNT          367 a          621 c          651 g          382 t
ORIGIN      
        1 attgatatgc tggcccgcat gatgcagatg ctgatgaacg acgactttgt cgaaaagctc
       61 cgtgccgcca agacgcccgc tgaatttttg gacgccatcg acgcgcagga ggatgcccag
      121 ttcggcgagg aaagctttac ccagcaggaa atcccccaga gcggctaccg cgtgctggcc
      181 gtcacagcct gccccaacgg cattgcccac acctacatgg ccgccgaggc cctgaccaag
      241 atgggggaca agctgggtct gcccacgaaa gttgaaacga acggatccga cggcgcgaaa
      301 aacgtgctga cgcccgagga aatcgccgcc tgcgacggta tcatcattgc ggcggacaag
      361 aatgtcgaga cggcccgctt tgacggcaag ccggtcattt ttgcccgcgt ggatgacggc
      421 atccacaaac cggaggagct tatcaagaaa attgctcacg gcgaagcgcc catctaccgc
      481 gccgaggccg gggccaaggc ccagcagagc gcaggcggtg cgagcgacag ccttggtcac
      541 agcctgtaca agcatctgat gaacggtgta tcgcatatgc tgcccttcgt tgtgggcggc
      601 ggcatcatga tcgcgctggc atttttgctg gacgattaca ccattgaccc ttccaacttt
      661 ggcatgaaca cgccggtggc ggccttcttc aagaccgtgg gcaacgcggc gttcggctat
      721 atgctgccga ttctggcagg ttttatcgcc atgtccattg cggaccgccc gggcctggcc
      781 gtcggctttg cgggcggcgt gctggccatg aacggcacga actttgcggg ccttgccagc
      841 ggtgacacca ccggaatttc cggcggtttt ctggcggcgc tgctggccgg tttcgccgcg
      901 ggctacattg tgcagttcct caagaaaatc accgagaagc tgcctgtcag cctgaacggc
      961 atccgcccga tgctgattta ccccttgggc ggtatcctcg tcactggttt ggtcatgtgc
     1021 ggcatcaacc cggtcatggg catcatcaac acggccatga ccgactggct gaacgccatg
     1081 ggcgacacgt ccaaggtgat gctgggcgca atcgtcgcgg gtatgatgag cattgacatg
     1141 ggcggccctt tcaacaaggc ggcttatgtg ttcggcacgg cggcgctggc tgccggcaac
     1201 tacgaggtca tggcggctgt tatggtcggc ggcatggtcc cgcccatcgc catcgcgctg
     1261 tccaccacct tctgccccaa aaagtggagc cccgacgagc gccgcaacgg cattgtcaac
     1321 tatgtcatgg gtctctgctt tgtctccgag ggagcgatcc cctatgcggc agcagacccg
     1381 ctgcgtgtgc tgcccagctg catcatcggc tcggcgctgg ccggttcgct ttcgatggtg
     1441 ttcggctgcg ccctgcgcgc accccacggc ggtatcttcg tgttcccggt cgtggatcat
     1501 gccctgctct acgtcatcgc cctggccgtc ggttcggcgg tgggcgcgct gattttgagc
     1561 ctgctgaaga aagagtacgt gaaagaataa gcacagaaac catgcaaggc cgccccggca
     1621 aaggggcggc cttttggcgt gccgtagggg aggagacggc gttcccggcg acccggcaac
     1681 gccgcagacg taaaaatccc cgctccctgt gccgcgggcg gcaggggagc ggggattgga
     1741 tattttgcgg aaaaatcagc ccaggctgat gcccatagcg cgggcgacgt tcagcatatc
     1801 gcagtcgccg ggcacgccgc gggtcttacc ggcaatgtcg gccagcgggt tggcggtgac
     1861 gtggcggttg accatggcga cggtcacgcc gtagtgctcg tccgccacca gcccggccgc
     1921 gtaggagcca aactgggttg ccagcacgcg gtcgtaggcg ctggggctgc cgccgcgctg
     1981 catgtggccg ggtacgcaga tgcgggtctc ggcaccggtc a
//
LOCUS       DBMDSQ010000354         2014 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_12227_length_2014_cov_1.263461, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000354 DBMDSQ010000000
VERSION     DBMDSQ010000354.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2014)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12227_length_2014_cov_1.263461"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..459
                     /gene="recR"
                     /locus_tag="ACZPAX_09935"
     CDS             <1..459
                     /gene="recR"
                     /locus_tag="ACZPAX_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005926735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HIR1114256.1"
                     /translation="AIEHAKKDLHRCRICQDYTAGEVCKICASQKRDHSVICVVESPR
                     DIKSIERTHEYNGLYHVLHGLISPMDGIGADQLCVKELLARLNDTVKEVIMATSPTVE
                     GEATAMYIAKLIKPLGIKTTRLAYGLPVGSSLEYADETTLYRAMAGRGEM"
     gene            531..998
                     /gene="smpB"
                     /locus_tag="ACZPAX_09940"
     CDS             531..998
                     /gene="smpB"
                     /locus_tag="ACZPAX_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HIR1114257.1"
                     /translation="MAEDKSGKKMIAVNREVRHEYFVVEALETGIELVGTEVKSLRAG
                     GVNLKDSWADIDDGELIAKGIHISPYEQGNIFNKDPRRPRRLLAHKAEIRRLGQQIKL
                     QGYTLVPLSLYFKKGRVKLELGLCKGKKLYDKRATAAARDAKRDIDRALKTQR"
     gene            1047..1399
                     /gene="ssrA"
                     /locus_tag="ACZPAX_09945"
     tmRNA           1047..1399
                     /gene="ssrA"
                     /locus_tag="ACZPAX_09945"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(1563..>2014)
                     /locus_tag="ACZPAX_09950"
     CDS             complement(1563..>2014)
                     /locus_tag="ACZPAX_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR1114258.1"
                     /translation="QRVLDARPDAVLLRATWMYDLPSYGLPIRGNLPLNLIKATMYGE
                     KLRFSSRDYRGVTYVRQAVENLVPAANLPGGVYNYGSENREDMVTTALAFAEALRLPD
                     ADNLIEAVDWQRNLAMDCGKLRGYGIRFAATQEGICHCLNDYGVADL"
BASE COUNT          462 a          572 c          565 g          415 t
ORIGIN      
        1 gccattgagc acgcaaagaa agacctgcac cgctgccgca tctgccagga ctatactgcc
       61 ggtgaggtct gtaaaatttg tgcctcccaa aagcgggacc acagcgtcat ctgcgttgtg
      121 gaaagcccgc gggatatcaa atccatcgaa cgcacccacg aatataacgg cctgtaccat
      181 gtgctgcacg gcctgatttc gccgatggac ggtatcggtg cagaccagct ctgcgtcaag
      241 gaactgctgg cccgcctgaa cgacaccgtc aaggaggtca tcatggctac cagtcccacg
      301 gtggagggcg aggcaaccgc tatgtatatc gccaaactca tcaagccgct gggcatcaag
      361 acgacccgct tggcctatgg tctgccggtg ggttccagtt tggaatatgc cgatgagacg
      421 acgctgtacc gcgctatggc cggtcgcggc gagatgtgac gaaaagccct tgacttttcg
      481 ccgcagccgc gttacaataa atatcctatc accgcgaagg aggtgtgccc atggccgagg
      541 acaaaagcgg aaagaaaatg attgccgtca accgtgaggt gcgccacgaa tactttgtgg
      601 tcgaagcgct ggaaaccggc attgagctgg tcggcaccga ggtcaaaagc ctgcgcgccg
      661 gtggggtaaa cctcaaggat tcctgggcgg atatcgatga cggcgagttg atagcaaaag
      721 gtatccacat cagcccctat gagcagggca acatcttcaa caaagacccg cgccgcccgc
      781 ggcggctgct ggcgcacaaa gcggagattc gccgcctggg ccagcagatc aagctgcaag
      841 ggtatacgct ggttcctctt tcgctctatt tcaaaaaagg gcgcgtcaag ctggaattgg
      901 gtctttgtaa aggcaaaaag ctctatgaca agcgtgcaac tgctgcggca cgcgatgcca
      961 agcgcgacat tgaccgcgca ctgaaaacgc agcgataagt tgttttccct tatctttccc
     1021 ggcaggctca cacctgccga atatgcgggg ctgtaatggt ttcgacgggg agagagaggc
     1081 gtgagcagcg ggccgtggcg gtgacccacg ataaaagcgc caacttaaat taactgacaa
     1141 caattatcct gttgccgtag ctgcctaatt aggctctacc gttctgcccg cgttagtacc
     1201 gcgcggggtc agggcgtcga ttaggtactg aacatgaacg ggcttgagct tggccgcccg
     1261 tcaggtatta acgaagctac caaggtgtag tgcgtgtatg ctcgcccgca ccaagggaaa
     1321 tccaataaac atactgcgcc cggagatact ctaactaatt tcttttcgga catgggttcg
     1381 actcccatca gctccataaa caaaggcccc cttgccgtgt cccggcaaga gggccttcgt
     1441 ttatggctaa gtctgatgaa gagccgaaca gggcggcgat gccgcagtgt cgggagcgac
     1501 agtcctacgg gctgtcgctc agcctgcggg ggacagccac tttacccgat tcttttacta
     1561 ggttataaat ccgcaacgcc gtagtcattc aagcaatggc aaatgccctc ctgcgtggca
     1621 gcaaacctta tcccatatcc gcgcagtttt ccgcaatcca tcgctagatt ccgctgccag
     1681 tccactgctt ctatcaggtt atctgcatcc ggcagccgca aagcctcagc aaaggctaaa
     1741 gcggtcgtca ccatatcctc tctgttttcg ctgccgtaat tgtatacgcc gccgggcagg
     1801 ttggcggcag gcaccaggtt ttctaccgcc tgccgcacgt aggtgacgcc acggtagtca
     1861 cggctagaga agcgcagttt ttctccatac atcgtcgctt ttatcaggtt cagcggcagg
     1921 ttgccccgga tgggtagtcc atagctgggc aggtcgtaca tccaggttgc acgcagcagc
     1981 acagcatcgg gccgggcatc cagcacgcgc tgtt
//
LOCUS       DBMDSQ010000355         2011 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_12248_length_2011_cov_1.545026, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000355 DBMDSQ010000000
VERSION     DBMDSQ010000355.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 2011)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2011)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2011
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12248_length_2011_cov_1.545026"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(127..906)
                     /locus_tag="ACZPAX_09955"
     CDS             complement(127..906)
                     /locus_tag="ACZPAX_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigB/SigF/SigG family RNA polymerase sigma
                     factor"
                     /protein_id="HIR1114259.1"
                     /translation="MYAGKVELCGVDTARLPVLSEAEKARLIKAARAGDRQARQEMVQ
                     GNLRLVLSVVQKFASRGENMDDLFQVGCIGLIKAIDNFDPAQNVRFSTYGVPMIVGEV
                     KRYLRDNNAVRVSRSIRDLAYHSMQAREELQKQNGREPKISEIAAKVGQSPENVAMAL
                     ESAVTPASLYEPVYSDGEDSFAMVDQLRDNAGEENWISDMMFRDTVKALTPRERRIIA
                     LRYLRGQTQTQVAQEIGISQAQVSRLEKAALKYIKEQICAD"
     gene            1027..1548
                     /locus_tag="ACZPAX_09960"
     CDS             1027..1548
                     /locus_tag="ACZPAX_09960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1114260.1"
                     /translation="MMDSLWFVIVLILLAVVSVALVLVLVLRREELRGGRETRTEYLQ
                     PNYGQTGGFRVASAPNNEIIIPYRYWLIEGCVSEIDYNIVPGRRMSLRTAKQGQMLYP
                     AGFFDLAFEDVQQYEIDGVTVTQRQNAGRITGISWARDGYEYLLYSMQPEMNMVVGLA
                     VEFVTNTRVEPVV"
     gene            complement(1662..>2011)
                     /locus_tag="ACZPAX_09965"
     CDS             complement(1662..>2011)
                     /locus_tag="ACZPAX_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR1114261.1"
                     /translation="YLRKSSNRRQDASIDEPLNTDNDGNELLLMDVLTSDDPQVGEEL
                     ERNAERAALRGAVQRLSPRERRIMELRFGLNHEEEHTQKEVADTLGISQSYISRLEKR
                     IIRRLRRELEAVG"
BASE COUNT          333 a          585 c          635 g          458 t
ORIGIN      
        1 atcaccggta ttttgtataa gaacccgaag tctttcaccc gtgcgctggg agcgtgcggg
       61 atcgagattg agtaaagcat aaatgacccg cggcgtccga tttggatgcc gcgggttttg
      121 ttttgcttag tccgcgcaga tttgctcttt aatatacttt agtgcggcct tttccaggcg
      181 gctgacctgg gcctgactga tgccgatttc ctgcgccacc tgggtctggg tctggccgcg
      241 gagataccgc agcgcgatga tgcgccgttc ccggggtgtc agggccttga ccgtgtcacg
      301 gaacatcata tcgctgatcc agttttcctc gcctgcgttg tcccgcagct gatccaccat
      361 ggcaaagctg tcctcaccgt cggagtagac cggctcgtac agactggcgg gcgtgacggc
      421 ggattccagc gccatggcga cgttttcggg gctttggccc accttggcgg cgatctcgga
      481 tattttcggc tcgcggccat tctgcttttg cagttcctcc cgcgcctgca tcgagtggta
      541 ggccaaatca cggatggagc ggctgaccct cacggcgttg ttgtcccgca ggtagcgctt
      601 gacctcgccc acgatcatcg gcacgccgta ggtggaaaac cgcacgttct gcgccgggtc
      661 aaagttatcg atggctttga taagcccgat gcagcccacc tggaacaggt cgtccatatt
      721 ctcgccgcgg cttgcgaatt tctgcacgac gctgagcacc agccgcaggt tgccctgcac
      781 catctcctgc cgggcctggc ggtcacccgc gcgggccgcc ttgataaggc gggctttttc
      841 ggcttcgctc agcactggca ggcgggcggt atccacgccg cagagttcta cttttccagc
      901 atacatcctg ttgaccccca gtttttattt accgggagta tgggccggta tgcgccgcgg
      961 caaaccggta aattttgcac attgggctgc ggcggcgcat agcatacgaa aaaggagggg
     1021 gtttttatga tggattccct gtggtttgtc attgtgctga tactgctggc cgtcgtgtcg
     1081 gtggcgctgg tcctggtgct ggttttgcgg cgggaggaac tgcgcggcgg ccgcgagacc
     1141 cgcaccgagt atttgcagcc caactatggg cagacgggtg gattccgggt cgcgtccgcg
     1201 cccaacaacg aaatcatcat tccctaccgc tactggctca tcgagggatg cgtctcggag
     1261 atcgattaca acattgtacc ggggcggcgg atgagcctgc gcaccgcgaa gcaggggcag
     1321 atgttatacc ccgccgggtt ctttgacctg gcgtttgagg atgtgcagca gtatgagatc
     1381 gacggcgtca cggtcacaca gcggcagaac gccgggcgta tcaccggcat cagctgggcg
     1441 cgggatgggt acgagtatct gctgtattca atgcagcccg aaatgaacat ggtcgtcggg
     1501 ctggccgtgg agtttgtgac aaacacccgg gtggagccgg tggtgtagaa ggcattgccc
     1561 tgtaggggcc gcacatgtgc ggcccgcggc tttccgggga ctcctgctga cgggcaggcc
     1621 gcgggccggg catgcccggc ccctacgaag ctgcagattt tttagcccac cgcctccagc
     1681 tcccggcgta atctgcggat gatgcgcttt tccagccgcg agatatagct ctgggatatg
     1741 cccaacgtgt cggcgacttc tttctgggtg tgttcttcct cgtggttcaa gccgaagcgc
     1801 agctccatga tacgccgttc ccggggggaa agccgctgca ctgcgccgcg cagggccgca
     1861 cgttcggcat tgcgttccag ttcttcgccc acctgggggt cgtcgctggt cagcacatcc
     1921 atcagcaaaa gttcgttgcc gtcgttgtcg gtgttcagcg gctcgtcgat gctggcatcc
     1981 tggcggcggt tggaactttt gcgcaggtac a
//
LOCUS       DBMDSQ010000356         2007 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_12287_length_2007_cov_1.690451, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000356 DBMDSQ010000000
VERSION     DBMDSQ010000356.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 2007)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2007)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2007
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12287_length_2007_cov_1.690451"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1781
                     /locus_tag="ACZPAX_09970"
     CDS             <1..1781
                     /locus_tag="ACZPAX_09970"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HIR1114262.1"
                     /translation="DSICGHGYAVLYVIAVRLVAYSNAGGYQVGSRGSVGSSAVAHFS
                     GISEVNSMPPHYLCPNCKHSEWINDGIHFDGFDLPDKNCPVCGKKMIVEGHDIPFETF
                     LGFYGDKEPDIDLNFSGMYQSHVHRYTEELFGKENVFKAGTVSGLQDKTAYGYVKKYL
                     EERGKTVNRAEENRLVMGCTGVKRTTGQHPGGMVVVPDTFDIYDFCAIQHPADDVKGG
                     LLTTHFEFKYLHDTLLKLDELGHDVPTFYKFFEEYSGIPIDSVPMNDEKVYSLLTSPA
                     ALGVTEEQIGSKTGTFGIPEMGTNFVRQMLLDAQPKNFSELIQISGLSHGTDVWTGNA
                     DELIRSGTCTIAEVIGCRDSIMLYLLRKGLEPKMSFDIMEAVRKGKVAKGGFKPGWEE
                     AMREHDVPDWYIESCRKIKYMFPKAHAVAYLMAAIRLMWFKVYHPAIFYAVYFTVRGA
                     DIDYEAAVGGVRVAKQHLKDNEKIPKDERTAKDDDALVSLQLENEMLQRGCEFLPIEL
                     GKSYASKYVVEDGKVRLPFTAIRGLGDAAAVALEQATMHGQEDISVEELQQASGVAQS
                     VFDKLRAVGALGNLPETSQVDLFSMI"
     gene            1864..1939
                     /locus_tag="ACZPAX_09975"
     tRNA            1864..1939
                     /locus_tag="ACZPAX_09975"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1897..1899,aa:Gly,seq:ccc)
BASE COUNT          432 a          590 c          580 g          405 t
ORIGIN      
        1 tggattccat ctgcggccac ggctacgcgg ttttgtacgt catcgccgtg cgtctggtgg
       61 cttactccaa tgcgggcggc taccaggtcg gcagccgtgg ttccgtcggt tcgtcggctg
      121 tggcacactt ctccggcatt tccgaggtca actcgatgcc gccgcattat ctctgcccca
      181 actgcaagca cagcgagtgg atcaacgacg gcatccactt tgacggcttt gacctgccgg
      241 acaaaaactg cccggtctgc ggcaaaaaaa tgatcgtcga aggccacgac attccctttg
      301 aaaccttcct gggcttctac ggcgacaagg aacccgatat cgacctgaac ttctcgggta
      361 tgtaccagtc ccacgtccac cgctataccg aggaactgtt cggcaaggaa aacgtcttta
      421 aggcgggcac cgtctccggt ttgcaggaca aaacggccta cggctacgtc aaaaagtacc
      481 tcgaggagcg cggcaagacc gtcaaccgtg ccgaggaaaa ccgccttgtc atgggctgca
      541 ccggcgtcaa gcgcacgacc ggccagcacc ccggcggcat ggtcgtcgta ccggatacgt
      601 ttgatatcta cgacttctgc gccatccagc acccggcgga cgatgtcaag ggcggcctgc
      661 tgaccaccca ctttgaattt aaatatctgc acgacacgct gctcaaactg gatgagctgg
      721 gccacgatgt gccgaccttc tacaaattct ttgaggaata ctccggcatc cccatcgact
      781 cggtcccgat gaacgacgag aaggtgtaca gcctgctgac cagcccggca gcgctgggcg
      841 tgaccgaaga gcaaatcggc tccaaaaccg gcaccttcgg catcccggaa atgggcacaa
      901 actttgtgcg tcagatgctt ttggatgcac agccgaagaa tttctccgag cttatccaga
      961 tttccggcct gtcccacggc accgatgtct ggaccggcaa cgccgacgaa cttatccgtt
     1021 cgggcacatg taccatcgcg gaggttatcg gctgccgtga tagtatcatg ctgtacctgc
     1081 tgcgcaaggg cctggaaccg aagatgtcct ttgacattat ggaggccgtg cgtaaaggca
     1141 aggtcgccaa aggaggcttt aagcccggct gggaggaagc catgcgcgag cacgacgtgc
     1201 ccgactggta tatcgaatcc tgccgcaaga tcaagtatat gttccccaag gcccacgccg
     1261 tggcctacct gatggcggcc atccggctga tgtggttcaa ggtctaccat ccggccatct
     1321 tctacgcggt gtacttcacg gtgcgcggcg cggatatcga ctacgaggcc gccgttggcg
     1381 gcgtccgcgt ggcaaagcag cacctgaagg acaacgagaa gatcccaaaa gacgaacgca
     1441 ccgccaagga cgatgacgcc cttgtcagtt tgcagctgga aaacgagatg ctgcagcgcg
     1501 gctgcgaatt tttgcccatt gaactgggta aaagctacgc cagcaagtac gtggtcgagg
     1561 acggcaaggt ccgtctgccg ttcacggcca tccgcggcct gggcgatgcc gctgctgtgg
     1621 cgctggaaca ggccacgatg cacggccaag aggatatctc ggtggaggaa ttgcagcagg
     1681 ccagcggcgt ggcgcagtcg gtctttgaca agctccgcgc tgtcggtgcc ctgggcaatt
     1741 tgcccgagac cagccaggtg gatttgttca gtatgatata aagggattgc ccatccgcgg
     1801 ggcgtcgggg acgccgcccc cttgacaacc cacctgaatt gcggtataat agcagtcact
     1861 gatgcgggat tagctcatcc ggtagagtga ctgcttccca agcagtaggt ggcgagttcg
     1921 agactcgtat cccgctccaa aataccctga agcatttcgc ttcagggtat tttttgttgc
     1981 gaggtgcgca gattatgctc gatgctt
//
LOCUS       DBMDSQ010000357         1990 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_12418_length_1990_cov_1.470619, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000357 DBMDSQ010000000
VERSION     DBMDSQ010000357.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 1990)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1990)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1990
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12418_length_1990_cov_1.470619"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..793)
                     /gene="rapZ"
                     /locus_tag="ACZPAX_09980"
     CDS             complement(<1..793)
                     /gene="rapZ"
                     /locus_tag="ACZPAX_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HIR1114263.1"
                     /translation="MNFLIVTGLSGAGKSMAVNALEDIGFFCIDNIPVALLPRIVDFA
                     LQGENQLSRVAVVMDVRGVRSIEQLKAALDDLDAKKIDYDILFLDANDSVIQRRYKET
                     RRQHPITISEKVPITEAIAKERKLLQPLREKAKYVIDTSLLSAAQNRERVCSLFLDKG
                     ESPMELMVVSFGFKYGLPQEADLVLDVRCLPNPFYVPELKHKTGLDQEVVDYVMNSEA
                     SQELLRRYESMLEYALPLYVKEGKSQLTIAVGCTGGKHRSITFARK"
     gene            complement(810..1736)
                     /gene="murB"
                     /locus_tag="ACZPAX_09985"
     CDS             complement(810..1736)
                     /gene="murB"
                     /locus_tag="ACZPAX_09985"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HIR1114264.1"
                     /translation="MQEQTITALRESLAAAGLPFVQDEPLAGHTTFKIGGPAAFWCTP
                     HNAEELRSTLALCRENGARVYLLGNGSNTLFDDAGFDGAVIDMRGVAEMEHSDADNDV
                     VRITAGAGQTLGRLCSKAQALGLTGLEFACGIPGTVGGAVYMNAGAYGGELKDVLESV
                     TFLDDNLQLRTLPAADLAMGYRTSIFEQNPGWCILSVTVVLHRGDGAAVLAKMQELLG
                     KRKEKQPLEWPSAGSTFKRPVGAFAGKLIEDCGLRGFTVGGAQISEKHCGFVINRGGA
                     TCADVVELTEQVKQIVKQKTGFVLEREIRVVK"
     gene            complement(1760..>1990)
                     /locus_tag="ACZPAX_09990"
     CDS             complement(1760..>1990)
                     /locus_tag="ACZPAX_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HIR1114265.1"
                     /translation="EYVGVGIANVINTFEPEVICIGGGVSNQGEVLLAPVRAYAENET
                     KNITTGKFPKICACTLHNDAGVIGAAALGNSI"
BASE COUNT          379 a          634 c          556 g          421 t
ORIGIN      
        1 tcttccgagc aaaggtaatg gagcggtgtt tgccgcccgt gcagcccacc gcgatggtca
       61 gctggctttt gccctccttg acatagagcg gcagggcgta ttccagcata ctctcatagc
      121 gtcgcagcag ctcctgcgac gcttcggagt tcatgacgta gtccacgacc tcctggtcga
      181 ggccagtctt gtgcttgagt tccggcacat agaaagggtt tggcaggcag cgcacatcca
      241 acaccaggtc tgcctcctgt ggcagaccgt acttgaagcc gaaggacacg accatcaact
      301 ccatcgggct ttcgcccttg tccagaaata ggctgcacac ccgctcacgg ttctgcgccg
      361 ccgaaagcag cgacgtgtcg atgacatatt ttgccttctc acgcaaaggc tgtaatagct
      421 tgcgctcctt ggcgatagcc tcggtgatgg ggactttctc gctgatggtg atggggtgct
      481 ggcggcgtgt ctccttgtag cggcgctgga tgacggaatc gttcgcatcc aggaacagga
      541 tgtcgtaatc gattttcttg gcatccaggt cgtccagagc ggctttcagc tgctcgatgc
      601 tgcgcacgcc gcgcacatcc ataacgaccg ccacgcggct gagctggttc tcgccttgca
      661 gggcaaagtc cacgatacgc ggcagcaatg ccactgggat attatcaatg cagaaaaagc
      721 cgatatcctc cagcgcattg acagccatag acttgcccgc gccggacagg ccggtcacga
      781 tcagaaagtt catttccacc accgctctct tactttacca cgcgaatctc acgttccagc
      841 acaaagccgg tcttttgctt cacgatttgt ttgacctgct cagtcagttc taccacgtcg
      901 gcacaggtcg cgccgccgcg gttgatgaca aagccgcaat gtttctcgct gatctgcgcg
      961 ccgcccacgg taaagccccg caggccgcag tcctcgatca gcttgcccgc aaacgcgccc
     1021 acgggccgct tgaaggtgct gcccgcgctg ggccactcca gcggctgctt ttccttgcgc
     1081 ttgcccagca gctcctgcat cttggcaagc acggcagcac catcgccgcg gtgcagaacg
     1141 accgtgaccg acaggatgca ccagccggga ttttgttcaa aaatactggt acgatagccc
     1201 atagccagat ccgccgcagg cagcgtacgc agctgtaagt tgtcatccag gaaggtcacg
     1261 ctttccagca cgtccttcaa ttcgccgccg taggcaccgg cgttcatata gaccgcgccg
     1321 cccaccgtgc cggggatgcc gcaggcaaat tccagtccgg tcaggcccag cgcttgggcc
     1381 ttgctgcaaa gccggcccag ggtctgccct gccccggcgg tgatgcgcac gacatcgttg
     1441 tctgcgtcgg agtgctccat ctcggccacg cctcgcatat cgatgactgc gccgtcaaac
     1501 cctgcatcgt caaacaatgt gttggagccg ttgcccagca ggtagacccg cgcgccgttt
     1561 tcgcggcaga gggccagcgt gctgcgcagt tcctccgcgt tatgcggcgt acaccagaac
     1621 gctgccgggc cgccgatttt gaatgtcgtg tgccctgcca gcggctcgtc ctgcacaaac
     1681 ggcagtcctg ccgccgccag tgattcacgc agtgccgtga tcgtttgttc ctgcatggaa
     1741 acctccgtaa aaactgctgt taaatagaat ttcccagggc ggctgcaccg ataacacccg
     1801 cgtcgttatg cagcgtgcag gcacagattt tggggaattt gccggtggtg atgttcttcg
     1861 tctcgttctc agcatatgcg cgaaccgggg ccagcaggac ttcgccctgg ttggacacgc
     1921 cgccgccgat gcaaatgacc tccggctcaa aggtgttgat gacgtttgca atgccgacgc
     1981 cgacatactc
//
LOCUS       DBMDSQ010000358         1989 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_12424_length_1989_cov_2.171610, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000358 DBMDSQ010000000
VERSION     DBMDSQ010000358.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 1989)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1989)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1989
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12424_length_1989_cov_2.171610"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..489
                     /locus_tag="ACZPAX_09995"
     CDS             <1..489
                     /locus_tag="ACZPAX_09995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HIR1114266.1"
                     /translation="GEGILILGDSGVGKSETAIELVKRGHRLIADDAVELRRVSYRKI
                     LGTAPANIRHFIELRGIGIINVARVFGIGSVRSSVEVEMVVQLEPWDRTKNYDRTGLE
                     TEYYDILGVKVPSMLIPVMPGRNLAVILETAAINNRQKEMGYNAAKELLTQLGLADDI
                     SK"
     gene            508..1257
                     /locus_tag="ACZPAX_10000"
     CDS             508..1257
                     /locus_tag="ACZPAX_10000"
                     /inference="COORDINATES: protein motif:HMM:NF014826.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HIR1114267.1"
                     /translation="MQMIADLHTHTLCATHAYNTFNEMAVAAKEIGYCALAVTDHAPA
                     MPDAPHSWHFSNWSAMPHVINDVVMLYGAEANVMDTKGGLDMPESQLKDQDWVVASIH
                     SSCVPGLLTRKEANRLWLAVAENPYVDIIGHSEQENYRYDYDLVTKAFAKNHKVVELN
                     GNSFNVRKDGIPNMRALLAACLQSGCHIAVDSDAHSTHQLKHGLTALYAMLEEMDFPQ
                     ELIVNATRENLVRELKLHKKPCAEKVGGILL"
     gene            1254..>1989
                     /locus_tag="ACZPAX_10005"
     CDS             1254..>1989
                     /locus_tag="ACZPAX_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HIR1114268.1"
                     /translation="MSKYTIGIDLGGTTMTAGLVNENYEIVAKTTWATRLPRPAEDLE
                     KALADLCRTVAKENKVDFADINYVGIGTPGSVNFKTGFVGFNTNFGYYDWNLGPDMEK
                     MLGCKVYVENDANAAAYGEYIAGGAKGYNDAVVITLGTGIGSGIILGGKILRGFNFAA
                     GEMGHNVIVKDGIQCTCGRKGCWERYASASALTRETKAAMQANKNTIMWTMTQDIDHV
                     NAKLAFDAMAKGDETAKQVVDSWIEYV"
BASE COUNT          471 a          583 c          557 g          378 t
ORIGIN      
        1 ggcgaaggca tcctgattct gggcgactcc ggcgtcggca agagcgagac ggccatcgag
       61 ttggtcaagc gcggccaccg tttgatcgcc gatgacgccg ttgagctgcg ccgggtgtcc
      121 taccgcaaaa tcctcggcac cgcacccgcc aatatccgcc actttattga gctgcgcggt
      181 attggcatta tcaatgtggc ccgcgtgttc ggtatcggtt cagtgcgttc cagcgttgag
      241 gtcgagatgg tcgttcagct cgaaccctgg gaccgcacaa agaactacga ccgcaccggc
      301 cttgagaccg agtattatga tatcctcggt gtcaaggtgc ccagcatgct catcccggtc
      361 atgcccggcc gtaacctggc tgttattctg gaaacggcgg ccatcaacaa ccgccagaag
      421 gaaatgggct acaacgctgc caaggagctg ctgacccagc tgggcctggc ggacgatatc
      481 tcaaaataaa agaggttttt ccattccatg caaatgattg cagacctgca cacccatacc
      541 ctgtgtgcga cccacgctta caacaccttt aacgaaatgg ccgtagcggc caaggaaatc
      601 ggttactgcg ccctggctgt gactgaccac gcgcccgcta tgcccgatgc gccgcactcg
      661 tggcatttct ccaactggag cgccatgccc catgtgatca acgatgtcgt tatgctctac
      721 ggagccgagg ccaatgtcat ggacactaaa ggcgggttgg atatgcctga gagccagctc
      781 aaagaccagg attgggtcgt ggcgtccatt cacagctcct gtgtgccggg cctgctgacc
      841 cgcaaggagg caaaccgcct ctggctggcc gtggcggaaa acccttacgt agatatcatc
      901 ggccattccg aacaggaaaa ttaccgctac gactacgacc ttgtcaccaa ggcgtttgcc
      961 aaaaatcaca aggtcgtgga gctgaacggc aactccttca atgtccgcaa ggacggcatc
     1021 cccaatatgc gcgctctgct ggcggcctgc ctgcaaagcg gctgccatat cgctgtggac
     1081 agcgatgccc acagcacaca ccagttgaaa cacggcctga cagccctcta cgccatgctg
     1141 gaagagatgg atttcccgca ggagctcatc gtgaacgcca cgcgggaaaa cttggtccgt
     1201 gaattgaaat tgcataaaaa accctgtgca gaaaaagtag gaggaatttt attatgagca
     1261 agtacactat tggtatcgat ctgggcggca ccaccatgac cgccggcttg gtcaacgaga
     1321 actacgaaat cgtcgccaag accacatggg ctacgcgcct gccccgcccg gcggaggatt
     1381 tggaaaaggc attggcagac ctgtgccgca ccgttgccaa agaaaacaaa gttgactttg
     1441 ccgatatcaa ctatgtgggc atcggaaccc ccggcagcgt gaacttcaag accggctttg
     1501 tcggcttcaa cacgaacttc ggctactatg actggaacct tggccccgac atggaaaaga
     1561 tgctgggctg caaggtctat gtggaaaatg atgccaacgc cgccgcttac ggcgagtaca
     1621 ttgcgggcgg cgccaagggt tataacgacg cagtcgtcat caccctgggc accggcatcg
     1681 gcagcggcat catcctgggc ggtaaaatcc tgcgcggctt caactttgcg gctggcgaaa
     1741 tgggccacaa cgtcatcgtt aaggacggca tccagtgcac ctgcggccgc aagggctgct
     1801 gggagcgcta tgcctctgcc agcgcactga cccgcgagac aaaggccgcc atgcaggcca
     1861 acaaaaacac catcatgtgg acgatgaccc aggatatcga ccatgtcaac gccaagctcg
     1921 cctttgacgc catggctaag ggcgatgaga cggcaaagca ggtcgtggac agctggatcg
     1981 agtatgtcg
//
LOCUS       DBMDSQ010000359         1984 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_12461_length_1984_cov_1.230483, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000359 DBMDSQ010000000
VERSION     DBMDSQ010000359.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 1984)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1984)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1984
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12461_length_1984_cov_1.230483"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(229..1689)
                     /gene="scfB"
                     /locus_tag="ACZPAX_10010"
     CDS             complement(229..1689)
                     /gene="scfB"
                     /locus_tag="ACZPAX_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0031179 - peptide modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioether cross-link-forming SCIFF peptide
                     maturase"
                     /protein_id="HIR1114269.1"
                     /translation="MIHQYKLSGYNIVLDVYSGSVHAVDDVAYDAIALIDQGLTREAA
                     TAQLEQKYRDRADVTPADISDCFDDIEELTAAGQLFAPDAYADHAFDFKNRSNVVKAL
                     CLHVAHTCNLNCSYCFAAQGKFHGEAGLMSFETGKRALDFLIEHSGTRRNLEVDFFGG
                     EPLMNFEVCKQLVAYARSIEKEHNKNFRFTMTTNGIGITDEVIDWCNKECHNVVLSLD
                     GRKEVNDRFRVDLAGNGSYDRIVPKFQKLVKARGGQGYYMRGTFTHHNVDFTKDLFHM
                     ADDLGFTELSMEPVVAAPDSPEALTEEDLPMLFDQYELLANDMLRRQKAGKPITFYHY
                     ILDLKHGPCIYKRISGCGSGTEYMAVTPWGDLYPCHQFVGDPNYKLGNVWDGVTNTVL
                     RDEFKLCNVYARPDCKDCWARLYCAGGCAANALHATGDIHGTYEYGCKVFRKRMECAL
                     MMQVAQRLDPELAKNAVKFESDCDGCGEGNDGACEK"
BASE COUNT          406 a          524 c          596 g          458 t
ORIGIN      
        1 cgcatccatc ccgcggcggt cgtacacagt ggtaaaatcg tatttcatag tgatacctca
       61 agaatcggtt agattgttga aaatatactt gtagaggtgt tctataccga aaggctcccc
      121 tcaagggtag ccattaaagg caggatatat aaatgctttt tccaaaatca cacgtatgtg
      181 tgattttatt taaagttctt tgcgtcgctt tctttcaaga aagcgacatt acttctcgca
      241 ggccccgtca tttccctcac cgcagccgtc gcagtcgctc tcaaatttca cggcgttctt
      301 ggcaagttcg gggtccaggc gctgggcgac ctgcatcatc agggcgcact ccatgcgctt
      361 gcggaacacc ttgcatccgt actcataggt gccgtggatg tcgccggtgg cgtgcagggc
      421 gttggccgcg cagccgcctg cgcagtacag gcgggcccag caatccttgc aatccgggcg
      481 ggcatacacg ttgcacagct tgaactcatc ccgcagtacg gtgttggtca cgccgtccca
      541 cacgttgccc agtttgtagt tggggtcgcc cacgaactgg tggcaggggt acaggtcgcc
      601 ccagggggtc acggccatgt actcggtgcc ggaaccgcag ccggaaatgc gcttgtagat
      661 gcaggggccg tgcttcaggt ccaggatata atggtagaac gtgatgggct tgccggcctt
      721 ctggcggcgc agcatgtcgt tggccagcag ctcgtactgg tcaaacagca tgggtagatc
      781 ctcctcggtc agggcctcgg gggaatccgg cgcggctacg accggctcca tggacagctc
      841 ggtaaagccg aggtcgtcgg ccatgtggaa caggtccttg gtaaaatcaa cgttgtggtg
      901 cgtaaaggtg ccgcgcatat agtagccctg gccgccgcgt gccttgacca gtttttggaa
      961 cttgggcacg atgcggtcgt agctgccgtt gccggccagg tccacgcgga accggtcgtt
     1021 gacttccttg cggccgtcca gagacagcac cacgttgtga cattccttgt tgcaccagtc
     1081 gatgacctca tcggtgatac cgatgccgtt ggtggtcatg gtgaagcgga agtttttgtt
     1141 gtgctctttc tcgatgctgc gggcataggc gaccagctgc ttgcacacct caaagttcat
     1201 cagcggttcg ccgccgaaga agtcgacttc caggttgcgg cgggtgccgc tgtgctcgat
     1261 caggaaatcc agcgcacgtt tgccggtctc aaagctcatc aggccggcct caccgtggaa
     1321 cttgccctgg gcggcgaagc aataactgca gttcaggttg caggtgtggg ccacatgcag
     1381 gcacagcgcc ttgacgacgt tggagcggtt cttgaagtcg aacgcatggt cggcataggc
     1441 gtcgggggca aacagctgcc cggcagcggt cagctcctca atgtcgtcaa agcaatcgct
     1501 gatgtcggcg ggggtgacgt cggcgcggtc acggtatttc tgctccagct gggcggtggc
     1561 ggcttcgcgg gtcaacccct ggtcgatcag ggcgatggca tcgtaggcaa catcgtctac
     1621 ggcgtgcacg ctgccgctgt acacgtccaa aacaatgttg tagccgctga gtttatactg
     1681 atgaatcatt gtctgctcct ttaagttatg ttacttttct tataagaaaa gtaaccaaaa
     1741 gattttttga tcgaatagcg agggtgtata gaaaagcggt cgagaataag ggatggggga
     1801 ggtgagggga aagtacggga aaagggatac tataaaatgc ctgcaccgtg cattgttttt
     1861 cggccgcact gtacatagaa aatgccgggc tgccatatct ggcaccccgg catcaaagcc
     1921 agcaatacaa ttacttgtcg gtcttctcgc aaggctggtt ggcaatgccg cagctggtct
     1981 tgca
//
LOCUS       DBMDSQ010000360         1983 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_12467_length_1983_cov_1.646121, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000360 DBMDSQ010000000
VERSION     DBMDSQ010000360.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 1983)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1983)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1983
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12467_length_1983_cov_1.646121"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1020)
                     /locus_tag="ACZPAX_10015"
     CDS             complement(<1..1020)
                     /locus_tag="ACZPAX_10015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1114270.1"
                     /translation="MPSKRTNDSVDRILEDLNIQQAQAGLRDSVTDHQVDEILRSVGI
                     STAPAQPVQGGFAFEADDDLGALLNDPGPAPSQAAPQPRQAASAPRPVQPAPQPTAPQ
                     TRPAAPATQPPRQDLPMADTGTTTFSGDTTGTGIIKGFLVKMAPEGSNADSDALNLGK
                     NQFQKFFKNSVAVVPDEKGRLREPGKKKRGLFGMKYADDTGEFVPINVSLGGGSTAPK
                     QPTPEPPAQEEYYPDEEPAPARQPAAKKHGFFGGLFGGEGETEELMIPEERVEEPTLS
                     RPAVTQPAPAEDTSTQEVWRSKYTRPARRAAEETESRTLERIQSTVGYAQQHPAPPSN
                     SGATGT"
     gene            complement(1056..1856)
                     /gene="rlmB"
                     /locus_tag="ACZPAX_10020"
     CDS             complement(1056..1856)
                     /gene="rlmB"
                     /locus_tag="ACZPAX_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HIR1114271.1"
                     /translation="MEKFARDRELAPDRAERTDTLVWGKNAVTELLKSGGAVDTVYLT
                     DTMPQAVAGYYTALSKESGAVVKRVPANKLQKMCGTPDHQGVAARAAEVEYANLDDLF
                     KAADAKGEQPFFVLCDGVEDPHNLGAIVRSAYLCGAHGVVIPKRGGVGVTGTVMKASA
                     GAAARLPIARVPNLAQAIRTIKEHNIFVYCADLGGAPLAKTDLSGPVALVLGSEGGGP
                     GALTRKLCDAAVTLEMAPGQATGVDSYNVSVAAGILCYDVMRRRIAKK"
BASE COUNT          282 a          632 c          663 g          406 t
ORIGIN      
        1 cgtgccggtc gcgccgctgt tggacggcgg cgcggggtgc tgctgggcgt agcccacagt
       61 gctctggatg cgctccagcg ttcggctttc ggtttcctcg gcggcgcggc gggccgggcg
      121 ggtgtacttg ctgcgccaaa cctcctgggt ggaggtatcc tccgccgggg cgggctgcgt
      181 caccgccggg cgggagagcg tcggttcctc cacgcgttcc tcggggatca tcagttcctc
      241 ggtctcccct tccccgccga acaggccgcc gaaaaagccg tgctttttgg ctgcgggctg
      301 gcgggcggga gcgggttcct cgtcggggta gtattcctcc tgcgcgggcg gttcgggcgt
      361 gggctgcttg ggcgcagtgc tgccgccgcc taacgagaca ttgatgggca caaattcccc
      421 ggtgtcgtcg gcgtatttca taccgaagag gccgcgcttt ttcttgccag gctcccgcag
      481 gcggcccttt tcatcgggca cgaccgcgac ggagtttttg aagaatttct ggaactggtt
      541 tttgcccagg ttcagcgcgt cgctgtcggc gttgctgccc tccggggcca tcttgaccag
      601 aaagcccttg atgatgccgg tgccggtcgt gtcgccgctg aaggtcgtcg tgccggtgtc
      661 cgccatcggc aggtcctgcc ggggcggctg ggtcgccggg gcggctggtc gtgtctgcgg
      721 ggccgtcggc tggggcgcag gctgcacagg gcgcggggcc gaggcggcct gccttggctg
      781 cggggcggcc tggctcggcg cagggccggg gtcgttcagc agggcgccca ggtcgtcgtc
      841 cgcctcaaag gcaaagccgc cctggaccgg ctgtgcaggc gcggtggaaa tgccgacgct
      901 gcgcaaaatc tcatcgacct ggtggtcggt gacactgtcc cgcagcccgg cctgcgcctg
      961 ctgtatattc aggtcttcca gaatacggtc aaccgaatca ttggtacgtt tgcttggcat
     1021 ggtgattcct ctttgtaaaa ggatgaactt cgtttttact tcttcgcaat ccgtctgcgc
     1081 atcacatcat agcacaggat gccggcggcg acgctgacgt tgtagctgtc cacgccggtc
     1141 gcctggccgg gggccatttc cagcgtcaca gcagcgtcac acagcttgcg ggtcagggca
     1201 ccggggccgc cgccctcgct gcccagaacc agcgccacag ggccggacag atcggtcttg
     1261 gccagcgggg caccgcccaa gtctgcgcag tagacaaaga tattatgctc tttgatcgtg
     1321 cggatggcct gcgccagatt gggcacccgg gcgatgggca gccgcgcggc agccccggcg
     1381 ctggctttca tgaccgtgcc tgtcacgccg acaccgcccc gcttggggat gaccacgccg
     1441 tgcgcaccgc acaggtaagc cgagcgcacg atagcaccca ggttgtgcgg gtcctcgaca
     1501 ccgtcacaca gcacaaaaaa cggctgctcg cctttcgcgt cggcggcctt gaacagatcg
     1561 tccaggttcg cgtattcgac ctcggccgcg cgggcagcca cgccctggtg gtccggcgtg
     1621 ccgcacatct tctgcaattt gttggcgggc acgcgcttga cgaccgcgcc gctctccttg
     1681 gacagggcgg tgtaatagcc cgccaccgcc tggggcatcg tgtcagtcag gtagaccgtg
     1741 tccaccgcgc cgccgctttt cagcagctca gtcacggcat ttttgcccca taccagggtg
     1801 tcggtgcgtt cagcacggtc gggtgccagt tcgcggtcgc gggcgaattt ttccatagtg
     1861 tcctccatct gtatcgtaaa aagccgacag ttatcgacta tgctctataa tcagataaca
     1921 gtatagcaca ctcagggggg ctttgtcatc tttttttgcg ccatttgcgg cccgccgggc
     1981 gca
//
LOCUS       DBMDSQ010000361         1980 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_12499_length_1980_cov_1.170836, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000361 DBMDSQ010000000
VERSION     DBMDSQ010000361.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 1980)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1980)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1980
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12499_length_1980_cov_1.170836"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            109..453
                     /locus_tag="ACZPAX_10025"
     CDS             109..453
                     /locus_tag="ACZPAX_10025"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024639.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR1114272.1"
                     /translation="MAVFSNKADEFARAVVARYFDSSLFEIVRGALPDVPTKPAPQGT
                     CALMQKLGVTAESGEVLYVGDSNVDVATAHNAGLPCCGVLWGFRTQEELQQAGAEYLA
                     ATMQDLEKVILQ"
     gene            512..>940
                     /locus_tag="ACZPAX_10030"
     CDS             512..>940
                     /locus_tag="ACZPAX_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007048403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR1114273.1"
                     /translation="MIAQHYKDMLGAKSVIRQISEWSTARGAEIGYENVFDYSLGNPS
                     VPAPQKFTDVCLDLLEHVDPVTLHGYTPTLTLPDVRAKVAESLNRRFGMDYTGDHIFM
                     TTGAAGALAHAVRCIAVPGQNIVTFAPFFPEYKPYVEGAGL"
     assembly_gap    941..950
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <951..1687
                     /locus_tag="ACZPAX_10035"
     CDS             <951..1687
                     /locus_tag="ACZPAX_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007048403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR1114274.1"
                     /translation="VNFDVLDSLVDENTAAMLINSPNNPSGTAYSAETLTKLAAYLKK
                     KSAEYGHHIFLISDEPYREIAFGGKTIPYPAKFYDDTLTCYSFSKSLSVPGERIGYVA
                     ANPRCEDAAYIVPMCGQISRGTGHNCPSGLFQRAVAECLEETSDLSVYETNMELIYDE
                     LKALGFTVCKPDGTFYIFPKALEKDAKVFCQKAMKYDLALVPGDSFGCPGYFRMAYCI
                     ETEKVRRSFVAFEKFVSTEYGVTKQH"
     gene            1697..>1980
                     /locus_tag="ACZPAX_10040"
     CDS             1697..>1980
                     /locus_tag="ACZPAX_10040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1114275.1"
                     /translation="MTIVRRILPVLLAAVLLSACGETKDVLPEIEQEVKQEVETVLEE
                     VEPTPEPTQTPEEAKTMASTASNATKDAPQVTDPSTWNDAGKTIMEALEK"
BASE COUNT          460 a          575 c          572 g          363 t
ORIGIN      
        1 tccgggcata taccggcctg cttcatacgc tagcagttca tccggagatc atgcaggaaa
       61 aaacggcaag tctggtaaca gcggaaattg caaaaaaacg gcgtaaagat ggcagttttc
      121 tcaaataaag ccgatgaatt cgcccgcgcc gtggtggcac ggtacttcga cagcagcctg
      181 tttgagatcg tgcgcggcgc actgcccgat gtgcccacca aacccgcacc ccagggcacc
      241 tgcgccctga tgcaaaagct gggcgtgacg gccgagagcg gggaagtgct ctacgtcggt
      301 gacagcaacg tggatgtggc aacggcccat aacgccgggc tgccctgctg cggcgtgcta
      361 tggggcttcc gcactcaaga ggaattgcag caggccgggg ctgagtacct ggccgcgacc
      421 atgcaagact tggaaaaggt gattttgcag taaaaatcaa aaaaacggga ttcgcaggaa
      481 gaacgcattt tcgcaaagga gtgttgttat tatgatcgca cagcattata aagacatgct
      541 gggggcaaaa tcggtcatcc gccagatctc ggagtggagc accgcccgtg gcgccgagat
      601 cggatacgag aatgtgttcg attattcgct gggcaacccc tcggtgcccg caccgcaaaa
      661 atttaccgat gtctgcctgg atctgctgga gcatgtggac ccggtcacgc tgcacggcta
      721 cacgcccaca ctgaccctgc ccgacgtgcg cgccaaggtg gccgaaagcc tgaaccgccg
      781 ctttggcatg gactacaccg gcgatcatat ctttatgacc accggcgccg ccggcgcgct
      841 ggcccatgcg gtgcgctgca ttgctgtgcc ggggcagaac atcgtcactt ttgcgccgtt
      901 cttcccggag tataagccct atgtggaggg tgcaggcctg nnnnnnnnnn aggtcaactt
      961 tgacgtgctg gacagcctgg tggacgagaa caccgccgcc atgctcatca actcgccaaa
     1021 taaccccagc ggcacggcct acagcgccga gacgctgact aagctggccg cttacctgaa
     1081 gaaaaagagc gccgagtatg ggcaccatat cttcctgatc tcggatgaac cctaccgcga
     1141 aatcgccttt ggcggcaaga ccatcccgta ccccgctaaa ttttacgatg atacgctgac
     1201 ctgctactcc ttctccaaga gtctgagtgt gccgggcgag cgtatcggct atgtggcggc
     1261 caacccccgc tgcgaggatg ccgcctacat cgtgccgatg tgcggccaga tctcccgcgg
     1321 caccggccat aactgcccgt cgggtctgtt ccagcgcgcc gtggccgaat gtctggagga
     1381 gaccagcgac ctgtctgtct acgagacgaa catggagctg atctacgatg agctgaaagc
     1441 cctgggattt acggtctgca agccggacgg caccttttac atcttcccga aagcgctgga
     1501 gaaggacgcc aaagtcttct gccagaaagc catgaaatat gacctggcgc tggtaccggg
     1561 cgacagcttc ggctgcccgg gctacttccg catggcctac tgcatcgaga ccgaaaaggt
     1621 ccgccgcagc tttgtggcct ttgaaaaatt cgtaagcacc gagtacggtg taacaaaaca
     1681 acattaagga gaaactatga cgattgtgcg ccgtattttg ccggtactgc tggcggctgt
     1741 gctgctttct gcctgcggag aaactaagga tgtgctcccg gaaatcgagc aggaagttaa
     1801 gcaggaagtg gagaccgtgt tggaggaagt agaacccacg ccggaaccca cccagacacc
     1861 cgaggaggcc aagactatgg catcgacggc cagcaacgcc accaaggatg ccccgcaggt
     1921 caccgaccct tccacctgga acgacgccgg caaaaccatc atggaggcgc tggaaaagca
//
LOCUS       DBMDSQ010000362         1973 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_12560_length_1973_cov_1.503205, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000362 DBMDSQ010000000
VERSION     DBMDSQ010000362.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 1973)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1973)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1973
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12560_length_1973_cov_1.503205"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            108..>1973
                     /locus_tag="ACZPAX_10045"
     CDS             108..>1973
                     /locus_tag="ACZPAX_10045"
                     /inference="COORDINATES: protein motif:HMM:NF014647.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR1114276.1"
                     /translation="MNRNEGNELYLKAQKLALRDYREKMLAGQSPFLPVLDDILQNVP
                     VENQIPLGQVDIPLNLLVGTKTTGRTAAFASNFMPLLDLKTEFASKWVNLCLSHLEEG
                     IRDPIRCYEYMGRFYVQEGNKRVSVLKYFDATSILGNVIRVVPQYSDDPAVQMYYEFM
                     HFYPITQNYLLTFTKPGSYARLQKILGKGPDEKWSGDDRAEVLSLYNWVEKAFLAHGG
                     AKLRCTVGDVLLLLLRVYTKEELAKLSPNELSEKLDALWDDVLALQKADPVRVSDKPA
                     EPKQTGLLDRILPGKHTAPSHLKVAFVHERTPGTSSWTSQHEFGRTQLDTVFAGQVET
                     TAYFNAVPGENADTLVEQAIADGADVVFTTSPKLVGASLRAAVKHPQVRILNCSMEMP
                     YASIRTYYTRVYEAKFITGAIAGAMAGTDRIGYVADYPSFGVPANINAFALGARMANP
                     RARIELLWTCLPDQADPLHTFTQKGITVISGRDAPMPNRPQREFGTFLVRPGGVLQDL
                     ATPFWHWGQFYENVIRTVLNGGWVRDKSGTDGRAVNYWWGMNSGVMDVLLSRELPPDV
                     NHLAQILRSGVTSGMIDPFHCRITAQDGSVKNSGRHGLDLEQIAHMDYLCDAVDGH"
BASE COUNT          413 a          620 c          562 g          378 t
ORIGIN      
        1 ccgaaggcgg tgcatacagg gtaattgcaa aaatctttgt atattccgca catatgtgtt
       61 ataataattt tgtatctgtt gtataagaat cgaggaggcg accggctgtg aaccgcaacg
      121 agggaaatga actgtacctg aaagcccaaa agctggcact gcgcgattac cgggagaaaa
      181 tgcttgccgg gcaatcgccg tttctgcctg tgctggacga tatcctgcaa aatgtccctg
      241 tggaaaatca aattcccctc ggccaggtgg atatcccgct gaatctgctg gtgggcacca
      301 aaacgactgg ccgcaccgca gcgttcgcat ccaactttat gccgctgctg gacctcaaga
      361 ccgagtttgc cagtaagtgg gtcaacctct gcctgtctca tctggaagag ggcatccgcg
      421 accccatccg ctgctatgag tacatgggtc gcttctatgt gcaggagggc aacaagcgcg
      481 tcagcgtgct gaaatacttt gacgcgacca gcattctggg caatgtcatc cgcgtcgtgc
      541 cgcagtacag cgacgacccc gccgtgcaga tgtactacga gttcatgcac ttttacccca
      601 tcacacagaa ctatctgctc accttcacca agcccggcag ctatgcccgc ctgcaaaaaa
      661 tcctgggcaa aggcccggat gaaaagtgga gcggcgacga ccgcgccgag gtattatccc
      721 tttacaactg ggtcgaaaag gcttttttgg cccacggcgg cgcaaaactg cgctgcacag
      781 tgggcgacgt actgctgctg ttgctgcgcg tttacactaa agaggagctt gccaagctca
      841 gccccaatga gttgagcgag aagctcgacg ccctgtggga cgatgtgctg gccctgcaaa
      901 aggctgaccc cgtgcgtgtc agcgacaagc ccgccgagcc gaagcagacc gggctgttgg
      961 atcgcatcct gcccggcaaa cacaccgcac ccagccactt gaaagttgcc tttgtccacg
     1021 agcgcacccc cgggaccagc tcctggacga gccagcatga gtttggacgt acccagttgg
     1081 acaccgtatt tgcgggccag gtcgagacga ccgcctactt caacgcagtc cccggtgaga
     1141 acgccgatac cttggtcgag caggccatcg ccgacggcgc agacgttgtt ttcaccacca
     1201 gccccaaact ggtgggtgcg tccttacgcg ccgccgtcaa gcacccgcag gttcgcattc
     1261 tgaactgctc gatggagatg ccctacgcca gcatccgcac ctattacacc cgcgtgtacg
     1321 aggccaagtt catcaccggc gcgattgccg gtgccatggc gggcacggac cgcatcggct
     1381 atgtggccga ctacccgagc tttggcgtac ccgccaacat caacgccttt gcgctgggtg
     1441 cccgcatggc gaacccccgc gcccgcattg agctgttgtg gacctgcctg cctgaccaag
     1501 ccgacccgct gcacaccttc acccagaagg gcatcacggt catttcgggc cgcgacgcac
     1561 cgatgcccaa ccgcccgcag cgggagttcg gcacattttt ggtgcgcccc ggcggcgtat
     1621 tgcaggacct ggctacgccg ttctggcact ggggccagtt ctacgagaat gtcatccgca
     1681 ccgttttgaa cggcggctgg gtgcgggata aatccggcac cgacggccgc gccgtcaact
     1741 attggtgggg catgaacagc ggcgtcatgg acgtgctgct cagccgcgaa ctgccgcccg
     1801 atgtcaacca tctggcacag atcctgcgtt ccggcgtcac ttccggtatg attgatccgt
     1861 tccactgccg catcacggcg caggacggca gcgtcaagaa cagcggccgc catgggctgg
     1921 acctggagca gatcgcccac atggactatc tgtgcgacgc cgtggacggg cac
//
LOCUS       DBMDSQ010000363         1951 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_12722_length_1951_cov_2.011351, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000363 DBMDSQ010000000
VERSION     DBMDSQ010000363.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 1951)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1951)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1951
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12722_length_1951_cov_2.011351"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(518..>1951)
                     /locus_tag="ACZPAX_10050"
     CDS             complement(518..>1951)
                     /locus_tag="ACZPAX_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005922369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR1114277.1"
                     /translation="HGDRLNGCLCSTRMNHRTDKFGGSLENRVRFARMLTRAIRKAVP
                     DMVIDYKLSIVTPQRGKGGIDEADAVQFAQWLVEDGVDMFHVAQANHTGNMADTIPPM
                     GVQPYGFFVKIAGDIKKAVNVPVSAVGRIVDADMAARVIESGMADIVAMGRPLLADPD
                     WGTKIAAGKACDIRRCISCNKGCTDAIQNRQFLSCVLNAENGYENTRSIQPAAQKKKI
                     AGLGGGPAGLEAARVAALRGHDVTLFEKTTTLGGQLNIACVPPRKEEMRRATQDLIHA
                     VCNAGVHLCMGQTRTAEQLKDAGFEAVINAVGAHSAAPRIPGIDSVNVADAWKVLAGE
                     QQVYGTVAVIGGGMVGCETAEYLAARGCKVSVIEMMDKIAAGESTTILPTLLENYKTY
                     GVEQYPSHKVKEFRMDAVVCENKDGAEVTIPCDYIVLAMGARSNAFDAAALEAAGIPV
                     YSIGDAAGKAADISNAIRTGYDTACQL"
BASE COUNT          346 a          583 c          631 g          391 t
ORIGIN      
        1 agcggaaaaa ttattttata atttcggaat accggagcgt ctgcggacgc tccggtattc
       61 cattttcacg ggaagggttt ggaacgaact ccctcagtca aagcctgacg gctttgccag
      121 ctccctctag gagggagcct ctgacgcgcc cgcaagcttt gcgctttagc cggaaactat
      181 accatcatgc caaaggccct atcccagagg acgacttccc ccggagcggg ggaagatgtc
      241 accgtaggtg acaaaagggg gaatctggcg cgggagcgcc tgagagggcg aacaaggcta
      301 gcgggtgctg cccctcatcc ggcgctacgc gccaccttcc cccggtgggt gaaggcttaa
      361 gcggtggcgg tacagttctc ggctgacccg taaagtttcc gggcgtgtcc gctctcccct
      421 tgggggagct ggcgcgttag cgcctgagag agtaaaaaaa gtgggtcaga agcgcccaca
      481 gacgtttctg acccacaaat agaacgatat ttttgtttta cagctggcag gcggtatcgt
      541 aaccggtgcg gatggcgttg gagatatccg cagccttacc ggcggcatcg ccgatggaat
      601 acaccgggat accggcagct tccagcgcag cagcgtcaaa tgcgttggag cgtgcgccca
      661 tggccagcac gatgtaatcg caggggatgg tcacctctgc gccgtccttg ttctcgcaga
      721 cgacggcgtc catgcggaat tccttgacct tgtggctggg gtactgctcc acgccgtagg
      781 tcttgtagtt ttccagcagg gtgggcagaa tggtggtgct ctcgcctgcg gcgatcttgt
      841 ccatcatctc gatgaccgac accttgcagc cgcgggcagc cagatactct gcggtctcgc
      901 agcctaccat gccgccgccg atgacagcca ccgtgccgta cacctgctgc tcaccggcca
      961 gaaccttcca agcgtcggca acgttcacgc tgtcgatgcc ggggatgcgg ggtgcggcgc
     1021 tgtgtgcgcc cacagcgttg atgacggcct cgaaaccggc gtccttcagc tgctctgcgg
     1081 tgcgggtctg acccatgcac aggtgcacac cggcgttgca gacagcgtgg atcaggtcct
     1141 gtgtggcgcg gcgcatctcc tccttgcggg gaggtacgca ggcaatgttc agctggccgc
     1201 ccagagtggt ggtcttttca aacagggtca cgtcatggcc gcgcagcgct gccacgcggg
     1261 cggcttccag accggcaggg ccgccgccga ggccggcaat cttctttttc tgtgctgcgg
     1321 gctggatgct gcgggtgttc tcgtagccat tctccgcgtt cagcacgcag gagaggaact
     1381 ggcggttctg gatggcatcg gtgcagccct tgttgcagct gatgcagcgg cggatatcgc
     1441 aggccttccc ggcagcgatc ttggtgcccc agtcggggtc tgccagcagc gggcgaccca
     1501 tggcaactat gtcggccatg ccgctctcga tgacgcgtgc agccatgtcg gcgtccacga
     1561 tacgacccac ggcgctgacc ggcacattga cggctttttt gatgtccccg gcgatcttga
     1621 caaagaagcc gtagggctgt acgcccatgg gaggaatggt gtcggccatg ttgccggtgt
     1681 ggttggcctg ggcaacgtgg aacatatcca cgccgtcctc caccagccac tgggcaaact
     1741 gcacggcgtc ggcttcgtca atgccgccct tgccgcgctg cggagtgacg atggacagct
     1801 tgtagtcgat gaccatatcc ggcacagcct tgcggatggc gcgggtcagc atacgggcaa
     1861 agcggacgcg gttctccaga ctgccgccga acttatcggt gcggtggttc atgcgggtgg
     1921 agcacaggca gccgttcagg cggtcgccgt g
//
LOCUS       DBMDSQ010000364         1950 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_12738_length_1950_cov_1.478096, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000364 DBMDSQ010000000
VERSION     DBMDSQ010000364.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 1950)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1950)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1950
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12738_length_1950_cov_1.478096"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..101)
                     /locus_tag="ACZPAX_10055"
     CDS             complement(<1..101)
                     /locus_tag="ACZPAX_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007591302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HIR1114278.1"
                     /translation="MAQKVTGYIKLQIEAGKATPAPPVGPALGQKGV"
     gene            complement(235..765)
                     /gene="nusG"
                     /locus_tag="ACZPAX_10060"
     CDS             complement(235..765)
                     /gene="nusG"
                     /locus_tag="ACZPAX_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015563687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HIR1114279.1"
                     /translation="MEEQTNEALWYVVHTYSGYENKVANDLQTMVENRHLQDLICDIK
                     VPTEMVPEIKDGKERMVEHKLFPGYVLVKMVMNDDTWYVVRNTRGCTGFVGPASKPVP
                     LSAEEVEKMGVEKAAPLTVDFNVGDTVQITAGPLEGFMGLVEGIDTESFKVKLKVNMF
                     GRETPAEVDIAQVELP"
     gene            complement(794..1033)
                     /gene="secE"
                     /locus_tag="ACZPAX_10065"
     CDS             complement(794..1033)
                     /gene="secE"
                     /locus_tag="ACZPAX_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015563686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HIR1114280.1"
                     /translation="MADKTEKKPGFVARMKGFGANIAKFFRDTKSELKKVVWPSKADV
                     KTNTIVVLVTVAIAAVVMIALDAIFGGILGLIIGA"
     gene            complement(1050..1199)
                     /gene="rpmG"
                     /locus_tag="ACZPAX_10070"
     CDS             complement(1050..1199)
                     /gene="rpmG"
                     /locus_tag="ACZPAX_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004402360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HIR1114281.1"
                     /translation="MTVKITLACTECKQRNYNTTKNKKNNPDRLEMKKYCRFCKKHTV
                     HRETK"
     gene            1506..>1950
                     /locus_tag="ACZPAX_10075"
     CDS             1506..>1950
                     /locus_tag="ACZPAX_10075"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005920503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HIR1114282.1"
                     /translation="MQSEDKLCIVVLFGGMSSEHEVSRVSAGTFVDNLDRARYEVLTV
                     GITREGRWLCTEATSAQMADGSWEELPGNMPCVISPDRADHGMILFTPSGQVEKLHVD
                     VVIPALHGLWGEDGTVQGLLELAGIPYVGCGVLASAVCMDKAVANA"
BASE COUNT          380 a          573 c          530 g          467 t
ORIGIN      
        1 ttgacgccct tctgacccag agcagggcca acagggggag ccggagtcgc cttgccggcc
       61 tcgatctgca gcttaatgta gccagtaact ttctgtgcca tagtaaatgc acctccaaaa
      121 atctgtggtg agttgcggcg tggtctcccc acgcctccca cgagcgcgta cacgatgcac
      181 acgctctata agaaccgcac tgcggtctta ccttggtaaa cggatgccgg ttggttacgg
      241 cagctcgacc tgcgcgatat ccacttctgc gggtgtctcg cggccaaaca tgttgacctt
      301 gagcttgact ttgaagctct cggtatcgat gccctccaca aggcccataa agccttccag
      361 cgggccggcg gtgatctgca cggtatcgcc gacattgaaa tcgacggtca gcggagccgc
      421 cttttcaacg cccatcttct ccacttcctc agcagagagg ggaacgggct tggatgcggg
      481 accgacgaaa ccggtgcagc cgcgcgtgtt gcgcaccaca taccaggtat cgtcgttcat
      541 gaccatcttg accagaacgt agccggggaa cagcttgtgc tccaccatac gctccttgcc
      601 gtccttgatc tcaggcacca tctcggtagg aaccttgatg tcacagatca ggtcctgcag
      661 atggcggttt tccaccatgg tctgtaggtc gttcgcaacc ttattctcgt aaccggaata
      721 ggtgtggact acataccaaa gggcctcatt ggtctgttct tccattttgg ttaagcctcc
      781 gtttcaagat gaatcatgcg ccgatgatca ggcccaggat gccgccgaaa atggcatcca
      841 gcgcgatcat cacaacggct gcgatcgcca cggtcacgag cacgacgatg gtgttggtct
      901 taacgtccgc cttagacggc cacacgacct tcttcagttc gctcttcgtg tcgcggaaga
      961 acttggcgat gtttgcgcca aagcccttca ttctggctac aaagcccggc tttttctcgg
     1021 ttttgtctgc catgatgcgt ccgcctttct tacttcgtct cgcggtgaac cgtgtgcttc
     1081 ttgcagaaac ggcagtactt cttcatctca agacgatcgg ggttgttctt cttgttcttc
     1141 gtggtgttgt agttgcgctg cttgcactcg gtgcaggcca gagtaatttt aacggtcatt
     1201 tgttgacacc tccattataa cggttgtttt gaacctccct cggcttgcgg tgcacgccct
     1261 aaaaacaggg cgcacttaat aaccccgcaa aagaggtgat atcattgtat cacgcgtttt
     1321 gccattcgtc aagcggattt tttgcgtttt tgagactttt ttgaattttt tctctcctgc
     1381 ctgttttggc ctacgccttg aatccgccgc atagatatag tataataaag tgtctataac
     1441 tgcttataga aaatccggcc ccgggcaaag cgccccttcc ggccataaga tcaggagaga
     1501 tcatgatgca atctgaagat aaactctgta tcgtcgtgct gtttggcggc atgtccagcg
     1561 agcacgaggt cagccgcgtg tccgccggta cctttgtgga taacctggac cgcgcccgct
     1621 acgaggtgct gaccgtgggc atcacccggg agggccgctg gctgtgcacc gaggccacca
     1681 gcgcccagat ggccgacggc agctgggaag agctgcccgg caacatgccc tgcgtcatca
     1741 gccccgaccg cgccgaccac ggcatgatcc tgttcacccc gtccggtcag gtggaaaagc
     1801 tgcacgtgga tgtggtcatc cccgcgctcc acggcctgtg gggcgaggac ggcaccgtgc
     1861 agggcctgct ggagctggcc ggcatcccct atgtgggctg cggcgtgctg gcaagcgctg
     1921 tctgcatgga caaggccgtg gccaacgccc
//
LOCUS       DBMDSQ010000365         1949 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_12743_length_1949_cov_2.001082, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000365 DBMDSQ010000000
VERSION     DBMDSQ010000365.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 1949)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1949)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1949
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12743_length_1949_cov_2.001082"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(1..101)
                     /locus_tag="ACZPAX_10080"
                     /pseudo
     CDS             complement(1..101)
                     /locus_tag="ACZPAX_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890790.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase
                     N-terminal domain-containing protein"
     gene            98..1949
                     /locus_tag="ACZPAX_10085"
                     /pseudo
     CDS             98..1949
                     /locus_tag="ACZPAX_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015538204.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
BASE COUNT          402 a          613 c          595 g          339 t
ORIGIN      
        1 ccatgatttt cggcaatgga catttcgttg tcgtttacga tgacgataat gttgctgccg
       61 agaacggccg cattgctcaa cccttccagc gcctcgccgc cacacgacgt tcggcacgat
      121 tctcgacgat gtgaagatcg acgacgagct ggtacaggcc gcgttcgacc gttacatggc
      181 tctgcgtgcc gagaccggcc gcgacaagaa gaaccgcacc ctgatggact gcgcgacgat
      241 tggtgccaag gccctgaagc agcgcggcat tctgaagaac ctcgacgaga gcgaggaaat
      301 caacgcctgc acggtcaaaa tcaagtgcga tgtggacggc gagcagcagg attggctgtt
      361 cctgttcaag aacgagaccc acaaccaccc caccgaaatt gagcctttcg gcggtgcggc
      421 aacctgcatc ggcggtgcca tccgcgatcc gctgtccggc cgcagttatg tttaccaggc
      481 catgcgtgtg accggtgcag ctgacccgct gaagcctgtt tccgagacga tgcccggcaa
      541 gctgccccag cgcaagctgg tcaccaccgc tgccgccggc tattccagct acggcaacca
      601 gattggcctt gccaccggtc aggtagccga gctgtaccac cccggctacg ccgccaagcg
      661 catggagatt ggcgcagtcg taggcgcaac gcccgccagc catgtacgcc gtgaggaacc
      721 cgcccccggt gatgtggtca tcctgctggg cggccgcacg ggtcgtgacg gcgtcggcgg
      781 cgcaacgggt tcttccaagg cgcacaaact gacgagcctg gaaacctgcg gtgccgaggt
      841 gcagaagggc aacgcgccca ttgagcgcaa actgcagcgc ctgttccgcc gcgaggatgc
      901 ctgccgcctc attaagcgct gcaacgactt cggcgcgggc ggtgtgtctg tcgccatcgg
      961 cgaactggct gacggtctgc atattgacct gaacaaagtc acccgcaagt atgagggcct
     1021 ggacggcacc gagctggcaa tttccgagag tcaggagcgc atggccgtgg ccgtggctgc
     1081 tgaggatgcc gagaccttta tgaagtacgc cgccgaggaa aacctcgagg cgaccatcgt
     1141 tgccaccgtc accgaggaaa aacggatgcg tgaggtatgg aacggcaaga caattgttga
     1201 cctgtcccgc gagttcctga actccaacgg tgccgagcgc cacgccaacg cccacatcct
     1261 gcccggccat gtctggcagc cccagtttgc cggtgtgagc tttgaagaaa aaatggagtc
     1321 tatggtcagt gacctgaacg tttgcagcca gaagggcctg ggcgagcgct tcgactccac
     1381 catcggcgca gccaccgtgc tgatgcctta cggcggcaag taccagctga ccccgaccat
     1441 ggccatggcc gccaagctgc ctgtggacgg cgagacaacg acctgctccg gcatggcgtg
     1501 gggcttcaac ccctacctga ccgaagctga cccctaccgc ggtgcctact tggccgtggt
     1561 ggagagcgtg accaagctgg tctgcgcggg cttccaccat gaggatatgt acctgacctt
     1621 ccaggaatat tttgagcaca tgaacgacaa gcccgagcgc tggggcaagc cgctggccgc
     1681 cctgctgggc gcactggatg cccagatggg cctggggatt gcgtccatcg gcggtaagga
     1741 ctccatgtct ggcagctttg aggggctgga cgtgcccccg acgctggtca gctttgcaac
     1801 ggccatcggc aacacccgcg acgtgcagag ccccgagttc aagaaagcca acagctcgat
     1861 cgtcatcttg cgccccaact acaagaacgg tctgcccgag atcggcagtc tgattgccat
     1921 ttacaagacc gtcgaacaga tgatcgacg
//
LOCUS       DBMDSQ010000366         1940 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_12830_length_1940_cov_1.308864, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000366 DBMDSQ010000000
VERSION     DBMDSQ010000366.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 1940)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1940)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1940
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12830_length_1940_cov_1.308864"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(154..1521)
                     /locus_tag="ACZPAX_10090"
     CDS             complement(154..1521)
                     /locus_tag="ACZPAX_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HIR1114283.1"
                     /translation="MEPEKQREGFGSRLGFILVSAGCAIGIGNVWRFPTVTGQYGGGI
                     FVLFYLIFLVLMGLPVLTMELAVGRAGHGAARSAYKALEPQGSKWHIHGWFCMVGCIL
                     LMMYYTTVSGWMLDYFFRFLTGNFDGLTSEQASGIFGEMLSSPVEMVFWMAIITVAGF
                     IVCGRGLQGGLEKVGKWMMSALLALILVLVVHSFTLSGAGDGLAFYLLPDWSRASEQG
                     LGNVITAAMNQSFFTLSLGIGAIEIFGSYMDRRFTLPGEAARICVLDTFVAVCAGLII
                     FPACFSFGISPDAGPSLIFITLPNVFTNMAGGRLWGTLFFLFMTFASFSTVIAVFENI
                     IACACEDFGWDRHKACIVGTAALVLLGLPCALGYNVWSFIQPMGAGSTVLDFEDFLVS
                     NLLLPGGSLVYLLFCVTEWGWGFDKYAAECNTGDGLKMPRWVKPYFKYVLPVVIAVIL
                     VQGLV"
     gene            1743..>1940
                     /locus_tag="ACZPAX_10095"
     CDS             1743..>1940
                     /locus_tag="ACZPAX_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low specificity L-threonine aldolase"
                     /protein_id="HIR1114284.1"
                     /translation="MLRFECDYGEGAAPAVLDLLQQTNFDQTPGYGEDEYCAKAAAQI
                     RSLCDAPDADVHFFVGATQANL"
BASE COUNT          455 a          588 c          554 g          343 t
ORIGIN      
        1 cggggctttc aacttcctta ttgggggtct cgggggcgtg cagccccccc cccgaggcgc
       61 gggtccgctg cgtcgcaaca gcggcagttt tctttgccag ctttcttttg ctgccaaaag
      121 aaagctgcct tcgggcaggc tgccgccaat cctttagacg agcccttgca ccaaaatcac
      181 cgcgatcacc accggcagca catacttgaa atacggcttg acccaccggg gcatcttgag
      241 gccgtcgccg gtgttgcact cggcagcata cttgtcaaag ccccagcccc attcggtgac
      301 acagaacaaa agatacacca gactgccgcc cggcagcagc aggttgctga cgaggaaatc
      361 ctcgaaatcc aacaccgtgc tgcctgcgcc catgggctgg ataaagctcc acacattgta
      421 gcccagcgca cagggcagac ccagcagcac cagcgccgcc gtgcccacga tgcaggcttt
      481 gtgacgatcc cagccaaagt cctcgcaagc gcaggcgatg atgttctcaa acactgcaat
      541 gaccgtggag aagctggcga aggtcatgaa cagaaagaac agtgtacccc acaggcggcc
      601 gcctgccatg ttcgtaaaga cgttgggcaa ggtaataaaa atcaagctcg ggcctgcgtc
      661 gggtgaaatg ccgaaggaga aacaggccgg gaagatgata agccctgcgc agaccgccac
      721 aaaggtgtcc agcacgcaga tacgcgcggc ctcgcccggc agggtaaagc ggcggtccat
      781 gtaactgccg aaaatttcaa tcgcaccgat gcccaggctc aaggtgaaga acgactgatt
      841 catcgctgcc gtgatgacgt tgcccaggcc ctgctcactg gcgcgggacc agtctggcag
      901 caggtagaag gccaggccgt cgcccgcacc ggacagggta aagctgtgga cgaccaatac
      961 gaggatgaga gcgagcagtg cactcatcat ccatttgccg accttttcca ggccgccttg
     1021 caggccgcgg ccgcagacga tgaagcctgc caccgtgata atagccatcc agaacaccat
     1081 ttccacggga ctcgagagca tctcgccaaa gatgccggac gcttgttccg acgtaaggcc
     1141 gtcaaaattg ccggtcagga agcggaagaa gtaatcgagc atccagccgg agacggtggt
     1201 gtagtacatc atcagcagta tacagcccac catgcagaac cagccgtgga tgtgccactt
     1261 gctgccctgc ggttccagcg ccttgtaagc gctgcgggcg gcaccgtgcc cggcgcggcc
     1321 gacggccagc tccatggtca gaaccggcag gcccatcagc accaggaaaa tcaggtagaa
     1381 caagacaaag ataccgccgc cgtactggcc tgtgacggtg gggaagcgcc agacgttgcc
     1441 gatgccgatg gcacagcccg cgctgacaag gatgaagcca aggcgtgagc cgaagccttc
     1501 ccgctgtttt tcgggttcca taatattcct cttttctcac aaagtactat tattatagcg
     1561 tagtacagcg aaacttgcaa gcgcggaatc acagtgttat aattagtgca acacccgccg
     1621 tgcggcgtag gggccgctgc ttgcatcggc ccgggcactt tgcagcagcc accgcactgc
     1681 gcgggcgaac aatgtttgcc cctactagca gcgtacatac taacgtgtaa ggagttaaga
     1741 atatgcttcg ttttgaatgt gactacggcg aaggcgcggc cccggccgtg ctggaccttt
     1801 tgcagcaaac caattttgac cagacccccg gctatggcga ggatgaatac tgcgccaagg
     1861 ctgctgcaca gatacgcagc ctgtgcgatg cccccgacgc cgatgtgcat ttctttgtcg
     1921 gcgcaacgca ggcgaacttg
//
LOCUS       DBMDSQ010000367         1937 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_12853_length_1937_cov_1.118736, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000367 DBMDSQ010000000
VERSION     DBMDSQ010000367.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 1937)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1937)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1937
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12853_length_1937_cov_1.118736"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(56..553)
                     /locus_tag="ACZPAX_10100"
     CDS             complement(56..553)
                     /locus_tag="ACZPAX_10100"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF013391.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - ATP-dependent diacylglycerol
                     kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HIR1114285.1"
                     /translation="MRSDLARFARGFVYAWNGIRAAVKEERNFRFHLCAAFYAFVAAY
                     WAGLSAVETALIALCVAAVMALELMNSAVERAVDKPDTTHWWTAGAAKDMAAGAVLVM
                     AIGTVIVAVCLFGPRLGVLWAAFTARWPRPVGILLSLLIFSKFIFQSSNSTQKGEQPN
                     GKTDS"
     gene            complement(543..1043)
                     /gene="ybeY"
                     /locus_tag="ACZPAX_10105"
     CDS             complement(543..1043)
                     /gene="ybeY"
                     /locus_tag="ACZPAX_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HIR1114286.1"
                     /translation="MSNKVLITNDQNVVKVPSGLRILIRRSCNAVLDFEHFDGPAEIS
                     VTFVDNNRIHELNREYRSKDSATDVLSFPLGENGEYDIDEDNGCKLLGDIVISMERAM
                     EQAELYGHSLQREVAYLTVHSMLHLLGYDHEAGGLEAVRMREKEEAVLIQLGLPRTVS
                     YTSDEI"
     gene            complement(1060..>1937)
                     /locus_tag="ACZPAX_10110"
     CDS             complement(1060..>1937)
                     /locus_tag="ACZPAX_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HIR1114287.1"
                     /translation="VCRGSVLRFTGEQKDVDAAARAVEGMVTLMENHTPLEEQTVRYC
                     MSLAHGGEEKRLRELTDDFVAVTAKGRPIHPKTLGQKEYLAAIRKNAITFGVGPAGTG
                     KTYLAVAMAVKAFKSKDVSRIILTRPAVEAGEKLGFLPGDLQNKVDPYLRPLYDGLFD
                     LLGAETFQKLFEKQVIEVAPLAYMRGRTLDDAFIILDEAQNTTPEQMKMFLTRMGVGS
                     KVVVTGDVTQIDLPDKVRSGLIDALHVLRNVEGIAQVRLTEKDVVRHRLVQQIVKAYE
                     QAAEEKSGSPEHKKV"
BASE COUNT          383 a          601 c          555 g          398 t
ORIGIN      
        1 ggatttgccc acgttgggcc gccccaccag cgccacaaag acactgctgg gcatttcagg
       61 aatcggtttt gccattcggt tgttcgcctt tctgtgttga attggaagat tggaagataa
      121 atttactaaa aatcagcagc gagagcaaaa tgcccaccgg gcggggccaa cgcgcggtaa
      181 atgcagccca cagcacgccc aaccgcggcc caaacaggca gacggccaca atgaccgtgc
      241 cgatggccat caccagtact gctccggccg ccatatcctt ggcggccccg gcggtccacc
      301 agtgggtggt gtcgggcttg tccacggcgc gctccacggc agagttcatt agctccagcg
      361 ccataacagc cgctacgcaa agcgcaatca gcgccgtttc cacggcactc agcccggccc
      421 agtaggccgc cacaaacgcg taaaaggcag cacagaggtg aaaacggaag ttccgttcct
      481 cttttactgc ggcgcggatg ccgttccatg cgtagacaaa gccgcgcgca aagcgcgcta
      541 aatcagatct catcgctggt gtaggaaacg gtgcgcggca ggcccagctg aatcagcaca
      601 gcttcttctt tctcgcgcat acgcacagcc tccagtccgc cggcctcgtg gtcgtagccc
      661 agcagatgca gcatcgagtg aacagtcagg tatgcaacct cgcgctgtag gctgtggccg
      721 tacagctcag cctgctccat ggcgcgctcc atcgagatga cgatgtcgcc cagcagcttg
      781 cagccgttgt cctcgtcgat atcatactcg ccgttttccc ccagagggaa gctgagcaca
      841 tcggtggcgg agtccttgct gcggtactcg cggttcagct cgtggatgcg gttgttatcc
      901 acgaatgtta cactgatctc ggccggaccg tcaaagtgct cgaaatccag cacagcgttg
      961 cagctgcggc ggatcaggat gcgcaagccg gaagggacct tgactacgtt ctggtcattg
     1021 gtaatcaaca ccttattgga cacggaaatc tacctccatt tatacttttt tatgctctgg
     1081 gctgccggac ttttcctcgg cagcctgttc atacgctttt acgatctgct ggaccaggcg
     1141 gtggcgcaca acatccttct cagtcaggcg cacctgggca atgccctcaa cgttgcgcag
     1201 gacatgcagc gcgtcaatca ggccgctgcg gaccttatcc ggcaaatcga tctgggtcac
     1261 gtcgccagtg acaacgacct tgctgccgac gcccatgcgc gttaagaaca tcttcatctg
     1321 ctcaggcgtg gtgttctggg cttcatccag aatgataaag gcgtcgtcca gcgtgcggcc
     1381 gcgcatatag gcaagtggcg cgacctcgat gacctgcttt tcaaacaatt tctgaaacgt
     1441 ctccgcgccc agcaggtcaa acaggccgtc atagagcggg cgcaggtatg ggtcaacttt
     1501 attttgcaag tcgccgggca gaaaccccag cttctcgccc gcttcgaccg ccgggcgggt
     1561 caggatgatg cggctgacat cttttgactt gaacgccttg acagccatcg ccacggccag
     1621 ataggttttg ccggtaccag ccgggccgac gccaaaggtg atggcgtttt tgcggatggc
     1681 cgccaggtat tccttctgcc ccagggtctt ggggtggatg gggcggccct tggcggtgac
     1741 ggcgacaaag tcgtcggtca gctcccgcag gcgtttttcc tcgccgccat gggccaggct
     1801 catgcagtag cgcacggtct gttcctccag cggggtgtgg ttttccatca gcgtgaccat
     1861 gccctccacc gcgcgggcgg cggcgtccac atccttctgc tcaccggtaa agcgcagcac
     1921 cgagccgcgg cataccg
//
LOCUS       DBMDSQ010000368         1932 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_12895_length_1932_cov_1.001092, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000368 DBMDSQ010000000
VERSION     DBMDSQ010000368.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 1932)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1932)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1932
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12895_length_1932_cov_1.001092"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..610)
                     /locus_tag="ACZPAX_10115"
     CDS             complement(<1..610)
                     /locus_tag="ACZPAX_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005944588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR1114288.1"
                     /translation="MIFLAATTVKVKEPKRSRALVRQETIASYLFLLPSLIFFLGFVI
                     YPMILCVVTSFFDSTMNRADVFVGLANYKELFADKIFLGALKNTFIIVIVSVPVTCAF
                     SLWVSSAIVDLPEWATSLFRCVFYLPVVTGSVAVTVVWKWMYNNYYGIFNYLGKTLGL
                     IDKNINWLGDERFALGCIILILLTTSVGQPIVLYVSALGNVDQ"
     gene            complement(745..>1932)
                     /locus_tag="ACZPAX_10120"
     CDS             complement(745..>1932)
                     /locus_tag="ACZPAX_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005920665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR1114289.1"
                     /translation="WTYPIGGWGKDETVQELISSFNAKYPDIKVTVEYLDYTNGDDQV
                     NTAIEGGSAPDLIMEGPERLVANWGKKGVMAPLNDLWTDDAKKDIYASVESACRNEAG
                     DYYEYPLCMTAHCMAVNMTKVKEVGADQYIDTDKHTWSTDGFLKTVDALYNGGYENVA
                     AIYCSGQGGDQGTRAIINNMYGGTFTDAEHTKYTADSPENIKAIQALYDAKGVNFDAS
                     INGGEEITLFRNGTLQMAFCWNIAQQLNSDNNDAGLTNSGDEIFPMAFPTESGDPKLC
                     GGIWGFGIFDNGDEAKIEAAKTFIDFIAADPDQVGASVLASTYFPVRESVGDIYAGND
                     IMSEYTKFMGFLGDYYQITPGWATARTEWWNMLQRVGTGEPVEQAVATFVENANAAAT
                     AEA"
BASE COUNT          419 a          518 c          582 g          413 t
ORIGIN      
        1 actggtccac gttgcccagg gcggaaacat acagaacgat gggctggccc acagaggtgg
       61 tcagcaggat gaggatgatg cagcccagag cgaaccgctc gtcgcccagc cagttgatgt
      121 tcttgtcgat taggcccagc gtcttgccca gatagttgaa gatgccgtag taattgttgt
      181 acatccactt ccagacaacg gtaacggcaa cggaaccggt gacaacgggc aggtagaaca
      241 cgcagcggaa aaggctggtg gcccactcgg gcagatccac gatggcggag ctgacccaca
      301 gcgagaaggc gcaggtcaca ggcaccgaca cgataacgat gatgaaggtg ttcttcagag
      361 cacccaggaa gatcttatcg gcgaacagct ctttgtagtt tgcaaggccg acaaagacat
      421 cggcgcggtt catggtggaa tcaaagaagc tcgtgaccac gcagaggatc atggggtaga
      481 tgacgaaccc cagaaagaag atcaggctgg gcagaaggaa gagataggat gcgatggtct
      541 cctgccggac cagtgcgcgg ctgcgctttg gctctttgac ttttacggtg gtagcagcca
      601 agaaaatcac caaacctttc gtttagctcc ggtaaaatga ccggctcccg acggaacttg
      661 caaaaagaat gcccctccct cgccgggatt atcgaggaag gggcaaggtt tataacgtta
      721 cgataaaaaa ctcaggtggt gcgcttatgc ctcggcagta gcggcagcat ttgcgttctc
      781 aacaaaggta gcaacggcct gctcaacggg ctcgccggtg ccaacgcgct gcagcatgtt
      841 ccaccactcg gtacgagcag ttgcccagcc cggggtgatc tggtagtaat cgcccaggaa
      901 gcccatgaac ttggtgtact cgctcataat gtcgttgccg gcgtagatgt cgccaacgct
      961 ctcacgaacg gggaagtagg tggaagccag aacgcttgcg ccaacctggt cggggtcagc
     1021 agcgatgaag tcgataaagg tcttggctgc ctcgatcttg gcctcgtcgc cgttgtcgaa
     1081 gatgccaaag ccccagatac cgccgcacag cttgggatcg ccgctctcag tggggaaggc
     1141 catggggaag atctcgtcgc cgctgttggt caggccggca tcgttgttgt cggagttcag
     1201 ctgctgtgca atgttccagc agaatgccat ctgcagagta ccgttgcgga acagggtgat
     1261 ctcttcgccg ccgttgatgg aagcatcaaa gttcacgccc ttggcatcgt acagagcctg
     1321 gatggccttg atgttctcgg gggagtcggc agtgtacttg gtgtgctcag catcggtgaa
     1381 ggtgccgccg tacatgttgt tgatgatcgc gcgggtgccc tggtcgccgc cctggccgct
     1441 gcagtagatg gctgcaacat tctcataacc gccattgtac agtgcgtcaa cggtcttgag
     1501 gaagccgtct gtgctccagg tgtgcttatc agtatcaatg tactggtctg cgcccacttc
     1561 cttgaccttg gtcatgttca cggccatgca gtgtgcggtc atgcacaggg gatactcata
     1621 gtagtcgccg gcttcgttcc tgcaggcgga ctcaacggaa gcgtagatgt ccttcttggc
     1681 atcgtcggtc cacaggtcat tcaggggagc cataacgccc ttcttgcccc agttggcaac
     1741 cagacgctcg ggaccttcca tgatcaggtc gggtgcgctg ccgccctcaa tggcggtgtt
     1801 gacctggtca tcaccattgg tgtaatccag gtactcaacg gtgaccttga tgtcgggata
     1861 ttttgcgttg aagctggaga tcagctcctg cacagtctcg tccttgcccc agccgccgat
     1921 ggggtatgtc ca
//
LOCUS       DBMDSQ010000369         1920 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_12993_length_1920_cov_1.620121, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000369 DBMDSQ010000000
VERSION     DBMDSQ010000369.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 1920)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1920)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1920
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12993_length_1920_cov_1.620121"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1183)
                     /gene="obgE"
                     /locus_tag="ACZPAX_10125"
     CDS             complement(<1..1183)
                     /gene="obgE"
                     /locus_tag="ACZPAX_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HIR1114290.1"
                     /translation="MATSNFIDTATIWLHAGKGGDGAVTFHREKFVAAGGPDGGDGGR
                     GGDIIFVADDNLSTLMDFRYKRKYTAPDGENGRAKRMSGADGDDLVIRVPRGTVLKEA
                     ETDLVVADLTGSEPVVVAKGGRGGWGNSHFATPTRQIPKFAKPGLPGEDMHLKLELKV
                     IADVGLIGFPNVGKSTLISIISAARPKIANYHFTTLTPVLGVVRVGPEQSFVCADIPG
                     LIEGAADGVGLGHDFLRHVERCRLLLHVVDVSGCEGRDPKADFEQINHELAGFSAELA
                     DRPQIVLGNKCDIATPEQVEEFKQYIEAKGLTFLPISAATRQGVDGLPALVYNRLKDL
                     PPVKVYEAEYKRPDKNAQPTRPFTVTRTGEHEFTVDAPWLERILAGTNVEDYESLQYF
                     QT"
     gene            complement(1244..1495)
                     /gene="rpmA"
                     /locus_tag="ACZPAX_10130"
     CDS             complement(1244..1495)
                     /gene="rpmA"
                     /locus_tag="ACZPAX_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005923362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HIR1114291.1"
                     /translation="MAHKKGVGSTKNGRDSESKRLGVKRGDGQFVLAGNILVRQRGTH
                     IHPGEGVGIGSDDTLFALVDGRVHFERMGRDRKRCTVKQ"
     gene            complement(1510..1821)
                     /gene="rplU"
                     /locus_tag="ACZPAX_10135"
     CDS             complement(1510..1821)
                     /gene="rplU"
                     /locus_tag="ACZPAX_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015563947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HIR1114292.1"
                     /translation="MYAVIKTGGKQYSVKVGDVVYVEKLNAEDNSEVTFDQVLAVGEE
                     GSVKVGAPVVEGASVVAKVVKTGKGKKLNILTYRPKKGSMVRKGHRQPYTKVEITAIN
                     G"
BASE COUNT          352 a          602 c          587 g          379 t
ORIGIN      
        1 gcgtctggaa atactgcagg ctctcgtagt cctcgacgtt ggtaccggcc agaatgcgct
       61 ccagccacgg cgcatcgacg gtgaactcgt gctcgccggt gcgggtgacg gtgaagggcc
      121 gcgtgggctg ggcgttcttg tcggggcgct tatactcggc ctcgtagacc ttgaccggag
      181 gcagatcctt caggcggttg tagaccagcg cgggcaggcc gtccaccccc tgccgcgttg
      241 cggcggagat gggcaggaag gtcagaccct tggcctcgat atactgcttg aactcctcga
      301 cctgctcggg ggtggcgata tcgcacttgt tgcccagcac gatctgcggg cggtcggcca
      361 gctcggcgct gaacccggcc agctcgtggt tgatctgctc aaagtcagcc ttggggtcac
      421 ggccctcgca gccggaaacg tccacgacgt gcagcagcag gcggcagcgc tcaacatggc
      481 gcaggaaatc atggcccaga ccgacgccgt ccgccgcacc ctcgatcagg cccgggatat
      541 cggcgcagac aaagctctgc tcggggccga cgcggacaac gcccagcacc ggggtcagcg
      601 tcgtaaagtg gtagttcgcg atcttggggc gcgcggcaga aataatgctg atgagcgtcg
      661 atttgccgac gttcgggaaa ccgatcaggc cgacgtccgc gatgaccttc aattccagct
      721 tgaggtgcat atcctcgccg ggcaggccgg gcttggcgaa cttggggatc tggcgggtcg
      781 gggtggcaaa gtggctgttg ccccagccgc cgcggccacc cttggccacg accaccggtt
      841 cggatccggt caggtcagcc acgacaaggt cggtctcggc ctccttcagc acagtaccgc
      901 ggggcacgcg gatgaccagg tcgtcgccgt cggcaccgct catgcgctta gcgcggccat
      961 tctcgccgtc gggcgcggtg tacttgcgct tatagcggaa gtccatcagg gtggacaggt
     1021 tgtcgtccgc cacaaagatg atatcaccgc cgcggccgcc atcgccgccg tccgggccgc
     1081 ccgccgccac aaatttttcg cggtggaacg tcaccgcacc gtcgccgccc ttgccggcgt
     1141 gcagccagat cgtggcggta tcaataaaat tactggttgc cataaaatac ctctttacta
     1201 caaaaaacga gcgagccgcg aaggcccgcc cgtaccggtt cttttactgc ttgaccgtgc
     1261 agcgcttgcg gtcacggccc atgcgctcaa agtgaacgcg gccatcgacc agagcgaaca
     1321 gggtgtcatc gctgccgatg ccgacgccct cacccggatg gatatgggtg ccgcgctgac
     1381 ggaccaggat gttgccggcc agaacgaact gaccgtcgcc gcgcttgacg ccgaggcgct
     1441 tggactcgga atcacgaccg ttcttggtag aacctacgcc ttttttatgt gccattgtga
     1501 tttacctcct tagccgttga tcgcggtgat ttccaccttg gtgtagggct gacgatgacc
     1561 cttgcggacc atgctgccct tcttgggacg ataggtcagg atgttgagct tcttgccctt
     1621 gccggtcttg acgaccttgg caacaacgga agcgccctca acaacgggag cgccaacctt
     1681 gacagagccc tcctcgccaa cggcgagaac ctgatcgaag gtgacttcgc tgttgtcctc
     1741 agcgttcagc ttctcgacgt agacgacgtc gccgacctta acgctgtact gcttgccacc
     1801 ggttttgata actgcgtaca tagtggataa cctctttcaa taagtctcgc tggttcgtag
     1861 ggcggcatac cgataaaagc acacacttac agagcccata ccatgcggct ctagtattct
//
LOCUS       DBMDSQ010000370         1912 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_13065_length_1912_cov_2.303147, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000370 DBMDSQ010000000
VERSION     DBMDSQ010000370.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 1912)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1912)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1912
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13065_length_1912_cov_2.303147"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            10..1179
                     /locus_tag="ACZPAX_10140"
     CDS             10..1179
                     /locus_tag="ACZPAX_10140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015565091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR1114293.1"
                     /translation="MDFTLSKQQQMVQKMYKEFAENEVKPLAKKVDAEEYFPKETVEK
                     MAKLGMMGIYFPKSVGGAGGDVLSYVMAVEEMSKVCGTTGVILSAHTSLCAAPIYENG
                     TPAQKEKYLPKLCSGEWIGAFGLTEPGAGTDAQGQQTFAVDEGDHWKLNGSKIFITNA
                     GFADVFIIIAVTGFVTDKRGRKQKEISAFIVERTDPGFKVGKPEDKMGIRGSSTCELI
                     MEDCIIPKDRLLGVKGKGFQLAMATLDGGRIGIASQALGIAEGAIDETVAYVKERKQF
                     GRSIAQFQNTQFELAEMKARVDAVKYLVYAAAQKKQAAMDGQKVRYSVEAAEAKLIAA
                     RTASDVTRRCLQLFGGYGYTRDYPIERMMRDAKITEIYEGTSEVQMMVISGALLK"
     gene            1194..>1912
                     /locus_tag="ACZPAX_10145"
     CDS             1194..>1912
                     /locus_tag="ACZPAX_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005940175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta"
                     /protein_id="HIR1114294.1"
                     /translation="MKAIVCVKQVPDTQGKVAVKADGTMDRSAMATITNPDDLNAVEA
                     ALQLKDETGCEVVVVTMGPPPAEGMLRELMARGADRGVLVSGREFGGSDTFATSQILA
                     AAVNKIGVGPEDIVFCGRQAIDGDTAQVGPQIAEKLHLPQVTYVTDIQKDGNSLTVKR
                     QLEDGYMELKVQTPCLLTCIKELNTPRYMSVSGIFECYDKPLEVFDYNALKDDPLIET
                     DTIGLKGSPTNVYKSFAPPVKG"
BASE COUNT          401 a          580 c          573 g          358 t
ORIGIN      
        1 gattacgtaa tggattttac tctgtctaaa cagcagcaaa tggtccagaa aatgtacaag
       61 gagtttgctg agaacgaagt caagcccctg gccaagaagg tcgacgcgga ggagtacttc
      121 cccaaagaga ccgttgagaa gatggctaag ctgggcatga tgggcattta tttcccgaag
      181 tccgttggcg gtgcaggcgg cgacgtgctg tcctacgtca tggcggttga ggaaatgtcc
      241 aaggtttgcg gcacgaccgg tgttatcctg tctgcccaca cctctctgtg cgcggcgcct
      301 atctacgaga acggcacccc cgcccagaag gaaaagtacc tgcccaagct gtgcagcggg
      361 gagtggatcg gcgctttcgg cctgaccgag cccggtgccg gtaccgacgc ccagggccag
      421 cagaccttcg ccgttgacga gggcgaccac tggaagctga acggctccaa gatcttcatc
      481 accaacgccg gttttgccga tgtgttcatc atcatcgccg tcaccggttt tgtcaccgac
      541 aagcgcggcc gcaagcagaa ggaaatcagc gccttcatcg ttgagcgcac tgatcccggc
      601 ttcaaggtcg gcaagcccga ggacaagatg ggcattcgcg gttcttccac ctgcgagctg
      661 atcatggaag actgcatcat cccgaaggat cgcctgctgg gcgtgaaggg caagggcttc
      721 cagctggcca tggctacgct ggacggcggc cgtatcggca tcgcttccca ggctctgggc
      781 attgccgagg gcgccatcga cgagactgtc gcctatgtca aggagcgcaa gcagtttggc
      841 cgctccatcg cacagttcca gaacacccag tttgagctgg ccgagatgaa ggcccgcgtt
      901 gacgctgtca agtatctggt ctacgcagct gcccagaaga agcaggctgc catggacggc
      961 cagaaggttc gttacagcgt cgaggctgct gaggccaagc tcattgccgc ccgcaccgcc
     1021 agcgacgtga cccgccgctg cctgcagctg ttcggcggct acggctacac ccgcgattac
     1081 cccatcgagc gcatgatgcg tgatgccaag atcaccgaga tctacgaggg caccagcgaa
     1141 gtgcagatga tggtcatcag cggcgcgctg ctgaagtaag gaggacagac gtaatgaaag
     1201 caatcgtttg tgtcaaacag gttcccgata cgcagggcaa ggtcgctgtc aaggctgacg
     1261 gcacgatgga ccgttccgca atggcgacca tcaccaaccc tgatgacctc aacgccgttg
     1321 aggccgccct gcagctgaaa gacgagaccg gctgcgaagt cgtcgtcgtc accatgggcc
     1381 cgccgccggc agagggtatg ctgcgtgagc tgatggcccg cggcgctgac cgcggcgtgc
     1441 tggtttccgg ccgcgagttc ggcggttccg acaccttcgc cacctctcag atcctggctg
     1501 ctgccgtcaa caagatcggc gttggccctg aggatatcgt tttctgcggc cgtcaggcca
     1561 tcgacggcga caccgcccag gtcggccccc agatcgctga gaagctgcac ctgccccagg
     1621 tcacctatgt taccgacatc cagaaggatg gcaactccct gactgtcaag cgtcagctgg
     1681 aggacggcta catggagctg aaggttcaga ctccctgcct gctgacctgc atcaaggagc
     1741 tgaatacccc gcgctacatg agcgtttccg gcatcttcga gtgctacgac aagccgctcg
     1801 aggtctttga ctacaacgct ttgaaggatg acccgctcat cgagaccgat accatcggcc
     1861 tgaagggctc cccgacgaac gtttacaagt cctttgctcc tccggtcaag gg
//
LOCUS       DBMDSQ010000371         1912 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_13066_length_1912_cov_1.868581, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000371 DBMDSQ010000000
VERSION     DBMDSQ010000371.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 1912)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1912)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1912
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13066_length_1912_cov_1.868581"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(177..1085)
                     /gene="pfkB"
                     /locus_tag="ACZPAX_10150"
     CDS             complement(177..1085)
                     /gene="pfkB"
                     /locus_tag="ACZPAX_10150"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005924968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HIR1114295.1"
                     /translation="MIYTVTFNPAIDYVVRLDRELAVGDVNRAKGEDCVLGGKGINVS
                     GVLAQLGCESVVLGFVAGETGAWLERGLVAQGLKTDFVHLDHGMTRINVKIKAGQETE
                     LNGAGPDIPESAMQALEEKLDRLQKGDILILAGSIPASLAQTTYERILARLEGRDIRA
                     VVDATRDLLVNVLRYKPFLIKPNNHELSEIAGVKLTNDNEIVTAARMMQAKGARNVLV
                     SMAGDGALLLDEQGGVHRIGCPKGKVVNSVGAGDSMVAGFVAGYQQSGGDYAAALKLG
                     TACGSATAFSLGLATKADIDKLLKTM"
     gene            complement(1082..1837)
                     /locus_tag="ACZPAX_10155"
     CDS             complement(1082..1837)
                     /locus_tag="ACZPAX_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005946479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HIR1114296.1"
                     /translation="MLAEERFALILNLLAEKRTATVQELCEALNASESTIRRDLTELD
                     RQGRLNKVHGGATLPDGQFLADEPTMAAKETLAVGQKRSIAQAAADLVRAGDFVYIDA
                     GSTTLAMVRALSGPALDAHYVTNGIAHARLLAQKSCNVCVPGGLLRPKTEAIIGAAAL
                     TSLQQFNFTKAFLGANGVALGAGFTTPDPEEAAVKAMASRRARETWFLVDDSKFGRVY
                     PAVISELYGGAILTNRCPEPKYKQLTLVKETEL"
BASE COUNT          304 a          606 c          585 g          417 t
ORIGIN      
        1 gaacagttcc gtgatacgca tacgatttgt ctccttcgtt tgtttctatt gaggtgtagg
       61 ggccgggcat acccggccct cggctttatg ggaaatgcgc atttatggct aagttgcggg
      121 ccgcacgtct ttgcaggagc ggatacttgc atcggcccct gcaagtcacg gtctactcac
      181 atcgtcttga gcagcttatc aatatccgct tttgtcgcca gtcccaggct gaacgcggtg
      241 gcactgccgc aggcggtgcc gagcttgagg gctgcggcgt agtcaccgcc gctctgctga
      301 taaccggcca caaaaccggc caccatgctg tcccccgcac cgacgctgtt gacgaccttg
      361 cccttcgggc agccgatgcg gtgtacaccg ccctgctcgt ccagcaacag cgcaccgtcg
      421 cccgccatgc tgaccagcac gttgcgcgcc cccttcgcct gcatcatcct ggcggcggtg
      481 acgatctcgt tatcgttcgt cagtttgacg ccggcgatct cgctcagttc gtggttgttc
      541 ggcttgatga ggaacggctt ataccgcagc acattcacca gcaaatcgcg ggtggcgtcc
      601 acgacggcac ggatgtcgcg gccttccagc cgggccagga tgcgctcata ggtcgtctgc
      661 gccaggctgg ccgggatgct gcccgccaga atcaggatat cgcccttttg caggcgatcg
      721 agcttttctt ccaacgcctg catggcgctt tcagggatgt cggggcctgc gccgttcagc
      781 tcggtctcct gcccggcctt gatcttgacg ttgatgcggg tcatgccgtg gtcgagatgg
      841 acgaagtcgg ttttcagccc ctgggcgacc aggccgcgct ccagccacgc accggtctcc
      901 cctgccacaa agccgaggac cacgctctcg caccccagct gcgccagcac accggacacg
      961 ttgatgccct tgccgcccag cacgcagtct tcgcctttgg cgcggttcac gtcgccgacg
     1021 gccaactcac ggtccagccg caccacgtaa tcgatggccg gattgaaggt cactgtatag
     1081 atcatagttc cgtctccttt accagtgtca gctgcttgta tttcggttcg gggcagcggt
     1141 tcgtcagaat cgcgccgccg taaagttcgc tgatgaccgc cgggtagacc cgcccgaact
     1201 tggagtcatc cacgaggaac caggtttccc gcgcacggcg ggaggccatg gccttgacgg
     1261 cagcctcctc ggggtcaggg gtcgtgaagc ctgcgcccag cgccacgccg ttggccccca
     1321 gaaaggcctt cgtgaagttg aactgctgta aactcgtcag tgccgccgcg ccgatgatgg
     1381 cctcggtctt ggggcgcagc agaccgccgg gcacgcagac gttgcagctt ttttgcgcca
     1441 gcagccgcgc gtgggcgatg ccgttggtga cgtagtgggc atccagcgcg gggccgctga
     1501 gggcacggac catagccagc gtggtggagc ctgcgtcgat gtagacgaaa tccccggcgc
     1561 ggacaaggtc ggctgccgcc tgagcgatgc tgcgcttttg gccgacagcc agcgtttcct
     1621 ttgcggccat cgtcggctcg tcagccagga attggccgtc cggcagtgtt gcgccgccgt
     1681 ggaccttgtt caagcggccc tgccggtcaa gttcggtcaa atctcggcgg atcgtggact
     1741 cgctggcgtt cagcgcctcg caaagctcct gcacggtggc ggtgcgtttc tccgccagca
     1801 ggtttaaaat caaggcaaag cgttcttcgg ctaacatggg gtgttgctcc tttcccggtc
     1861 attgtttgac tgtttttggt gatttatgat gttttatgac tggctgtatc tg
//
LOCUS       DBMDSQ010000372         1892 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_13250_length_1892_cov_1.352317, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000372 DBMDSQ010000000
VERSION     DBMDSQ010000372.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 1892)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1892)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1892
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13250_length_1892_cov_1.352317"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(167..1315)
                     /locus_tag="ACZPAX_10160"
     CDS             complement(167..1315)
                     /locus_tag="ACZPAX_10160"
                     /inference="COORDINATES: protein motif:HMM:NF018591.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YqfD"
                     /protein_id="HIR1114297.1"
                     /translation="MKAEFWAADTFRFSVTGPGAFRFLNRAAASGVRLRRVRWQKDGY
                     TATASGPDWRRLEEIAKSGGWAFTVTARRGPGRRTEWLLRTRPGLAAGAVLFFVLLRF
                     LGGFVWCIDYGAMDTDLHAGVRTLLADCGIHEGVYLTKPLLQAAQAQALRRSETFGWV
                     SLNFTGGCLSVESTPAQTQTVRDPPSRQGLYAAADGEVLAVEIESGFAVVAVGERVTA
                     GQPLAAAEKLDRKGNAVPQGAWGRIVARVQKQYTTAQALEYEALVYTGRSSAAETLYL
                     PGYAHTQPAETPLAGEEQTEWLPLRLGRLALPACICRVTTWEQQTQPVRLTDTAAVAL
                     AVRTCRAQLLRDFPDAEIEAEQRETTTENGTAAAAVTYIFTADIARAR"
     gene            complement(1305..1622)
                     /locus_tag="ACZPAX_10165"
     CDS             complement(1305..1622)
                     /locus_tag="ACZPAX_10165"
                     /inference="COORDINATES: protein motif:HMM:NF019488.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YabP/YqfC family sporulation protein"
                     /protein_id="HIR1114298.1"
                     /translation="MKPKKPHPRKPYERPDLRRMAAPPPRDFYRLPELELRGGNLLTD
                     GCRRVLDFTPQRVCLDMGDTLVTLYGEGLRIESFAGKRLILAGQFTRVELRSKWEVPP
                     DEG"
     gene            1680..>1892
                     /locus_tag="ACZPAX_10170"
     CDS             1680..>1892
                     /locus_tag="ACZPAX_10170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1114299.1"
                     /translation="MNVCRNRRFFTAAAAAVLGVLVLAEPQAAKQGFASGTALCLDRV
                     LPALFPFFVVCELLMAAPPPAVLLRPL"
BASE COUNT          321 a          613 c          590 g          368 t
ORIGIN      
        1 tgtccagttc aagagatcgt tctgccaata tacgttcctc catgggatag ttggttatag
       61 gcccagctat acatttagat tatatcacag ttttccgcaa aagaaactgt gaatattgca
      121 caaaattttg cgtttcattt cgtcactgtc cgcaaaaatt gtcgaattac cgcgcccggg
      181 caatgtcggc ggtaaaaata taggtcacag cggcggcagc ggtgccgttt tcagtggtgg
      241 tttcacgctg ttcggcttcg atttccgcat ccgggaagtc ccgcaaaagc tgggcgcggc
      301 aggtgcggac agccagcgcg acggctgcgg tgtcggtcag gcgcaccggc tgggtctgct
      361 gctcccaggt cgtcacgcgg cagatacagg cgggcagcgc caggcggccc agccgcagcg
      421 gaagccactc ggtctgttcc tcgccggcca gcggcgtttc ggcgggctgg gtgtgagcat
      481 agcctggcaa gtagagcgtt tccgccgcac tgctgcggcc ggtgtagaca agggcctcgt
      541 attccaacgc ctgggcggtg gtgtattgtt tttgcacccg cgccacaatg cgcccccatg
      601 ccccctgggg tacggcgttg cccttgcggt cgagcttttc cgccgccgca agaggctggc
      661 ccgccgtgac ccgctccccc actgcgacca ccgcaaagcc gctctcaatt tccaccgcca
      721 gtacctcccc atctgcggca gcgtacaggc cctgccgtga cggtgggtcg cgcacggtct
      781 gggtctgggc gggcgtactc tccaccgaaa ggcagccgcc ggtaaagttc aggctgaccc
      841 agccgaacgt ctcgctgcgg cgcagcgcct gggcctgggc cgcctgcaaa agcggtttgg
      901 tcaggtacac gccctcgtgg atgccgcagt ccgccagcag tgtgcgcacg cctgcgtgca
      961 ggtcagtgtc catcgcgccg taatcgatgc accagacaaa gccgcccaga aaacgcagca
     1021 gcacaaaaaa cagcaccgca cccgccgcca gcccggggcg ggtgcgcagc agccattcgg
     1081 tgcgccgccc gggaccgcgc cgggccgtga ccgtaaacgc ccagccgccg ctttttgcaa
     1141 tttcttccag tctgcgccag tccgggccgc tggctgtggc ggtgtagccg tctttctgcc
     1201 agcggacgcg ccgcagccgc acgccgctgg ccgccgcgcg gttcaaaaat cgaaacgccc
     1261 ccggcccggt cacggaaaag cggaaggtat ccgccgccca aaattcagcc ttcatcgggt
     1321 ggcacctccc acttgctgcg cagttccacc cgggtaaact gcccggccag gatcaatctt
     1381 ttccccgcga aggattctat ccgcagtccc tcgccataca atgttaccag ggtgtcgccc
     1441 atgtccaggc agacgcgctg cggggtgaaa tccagcacgc ggcggcagcc atctgtcaac
     1501 aggttgccgc cgcgcagttc cagctccggc aggcggtaga aatcccgggg cggcggggcg
     1561 gccatgcggc gcagatcggg gcgctcatac ggtttgcggg ggtgcggctt tttcggcttc
     1621 atggctgttc cctccccgca aaaatctgtc agtccagtat acgcggtgag gtgcgcaata
     1681 tgaacgtttg cagaaatcgg cgttttttta cagcggccgc ggcggctgta ctgggggtat
     1741 tggtactggc cgagccgcag gccgcgaaac aggggtttgc cagcggcacg gcactctgtc
     1801 ttgacagggt gctgcctgcg ctgtttcctt tttttgtggt gtgtgaactg ctgatggccg
     1861 ccccaccgcc cgccgtgctg ctgcggcctt tg
//
LOCUS       DBMDSQ010000373         1890 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_13272_length_1890_cov_1.204584, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000373 DBMDSQ010000000
VERSION     DBMDSQ010000373.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 1890)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1890)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1890
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13272_length_1890_cov_1.204584"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..627
                     /locus_tag="ACZPAX_10175"
     CDS             <1..627
                     /locus_tag="ACZPAX_10175"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007050743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HIR1114300.1"
                     /translation="NFDYPYLACSIGEYWRRWHMSLSGWFRDYVYIPLGGSRCRLRRT
                     CLNLLIVFTLTGLWHGAAWQYVVFGLLHGLILCAERLGLRRALERLPRFFGHLYTVMV
                     LWLTLVIFGAPGLKEGLAALWGIFTWQSGAKGYTLAAFADAKLLLILAVSFLLCGPLQ
                     ALRPKLKEALYTRKAPSLAGAAGLLVLLFFGLMRVTAGTYSAFIYFQF"
     gene            643..1704
                     /locus_tag="ACZPAX_10180"
     CDS             643..1704
                     /locus_tag="ACZPAX_10180"
                     /inference="COORDINATES: protein motif:HMM:NF028132.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate O-acetyltransferase AlgX-related
                     protein"
                     /protein_id="HIR1114301.1"
                     /translation="MRKAGKIAFVGAFCAVIGLAFPVFNVFSKQLSMDSHENRLMTGL
                     PQVLNSPLRELPRNLDNFLVDNSPFRYQFVLVNAGLDYAMFGTSQSDQVLPGKQGWLF
                     YKDGPNAAQPMANYQGLTELNDSAEVLAEASAGLQILNDRLAANGCTLVLDLTPSKDR
                     IYREYMPDGYPIVNEQNRTDLMAAYLQSHTTVPVVWRYDVLRSQARQNPERLLYYKTD
                     THWNSIGALIGLDGIFEALDMQTLSPDAYPVEADGTTTGDMANVAALYASLPAEETYT
                     VPGYAQLFEKDGRAVRVIGDSFSEYYMPYLQARFTNSWREHIDTFTMDMVDHPGCDIL
                     ILEFNERSLDKLLAILEAF"
     gene            complement(1701..>1890)
                     /locus_tag="ACZPAX_10185"
     CDS             complement(1701..>1890)
                     /locus_tag="ACZPAX_10185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1114302.1"
                     /translation="FMRKTVRTVRLNNLLEEMLKSDKVRIQYASKYALHHQLSRIFLI
                     TLQKTACLRSFRRQAVPI"
BASE COUNT          345 a          550 c          587 g          408 t
ORIGIN      
        1 aacttcgact acccctatct cgcctgctcc atcggcgaat actggcggcg gtggcatatg
       61 tcgctgtccg gctggttccg cgactatgtg tacatcccgc tgggcggcag ccgttgccgt
      121 ctgcgccgca cctgcctgaa cctgctcatt gtgtttaccc tcaccggcct gtggcacggc
      181 gcggcgtggc agtatgtggt gttcggcctg ctgcacggcc tgattttatg tgccgagcgc
      241 cttggcctgc gccgggcgct ggaacggctg ccccggttct tcggccacct gtacaccgtc
      301 atggtgctgt ggctgacgct ggtcatcttc ggtgcgccgg gcctgaaaga aggcctggct
      361 gcgctgtggg gcatctttac ctggcagagc ggggccaagg gctacacgct ggccgccttt
      421 gcggatgcca agctgctgct gattctggcg gtgtccttcc tgctgtgcgg gccgctgcag
      481 gcgctgcgcc ccaagctgaa agaagccctt tacacccgca aagcgccgtc cctggccggg
      541 gcggcggggc tgttggtgct gctgttcttt ggcctgatgc gggtcacggc gggcacgtac
      601 agcgcgttca tctatttcca attttaaagg agggtgcaga ccatgcgtaa agcgggcaaa
      661 attgcctttg tgggcgcgtt ctgcgccgtc atcgggctgg cgttcccggt gttcaatgtg
      721 ttctccaaac agctgtcgat ggacagccac gaaaaccgcc tgatgaccgg cctgccccag
      781 gtgctgaaca gcccgctgcg ggagctgccg cggaatctcg ataactttct ggtggacaat
      841 tctcccttcc gctaccagtt tgtgctggtc aacgccgggc tggactatgc gatgttcggc
      901 acgagccaga gcgaccaggt gctgcccggc aagcagggct ggctgttcta caaggatggg
      961 cctaacgctg cccagccgat ggcaaattat cagggcctga ctgagctgaa tgacagtgcc
     1021 gaggtgctgg ccgaggccag cgcggggctg cagatcttaa atgaccggct ggcggccaac
     1081 ggctgtacgc tggtgttgga cctgacgcct agcaaggacc gcatctatcg cgagtacatg
     1141 cccgacggct accccatcgt gaacgagcag aaccgcaccg acctgatggc ggcttacctg
     1201 caaagccaca cgacggtgcc ggtggtgtgg cggtacgatg tgctgcgcag ccaggcacgg
     1261 cagaaccccg aacggctgct ctactacaag accgacacgc actggaactc catcggtgcg
     1321 ctgattgggc tggacggtat ttttgaggcg ctggacatgc agaccctctc ccctgacgcc
     1381 tacccggtgg aggccgacgg cacgacgacc ggcgacatgg cgaatgtggc ggcgctgtat
     1441 gccagtctgc ccgccgagga gacttacacc gtacccggct acgcccagct gtttgaaaaa
     1501 gacggccgtg cggtgcgggt catcggggat tccttcagtg agtattatat gccctatttg
     1561 caggcgcggt tcaccaacag ctggcgcgag cacatcgaca cgtttacgat ggatatggtc
     1621 gaccacccgg ggtgcgatat cctgattttg gaatttaacg agcgcagcct ggataagctg
     1681 ctggcaattc tggaagcatt ttaaatagga acggcctgcc tgcggaaact acgcagacag
     1741 gccgtttttt gcaatgtaat caaaaatata cgcgatagtt gatggtgtaa tgcatacttg
     1801 ctggcatact ggatgcgtac tttatcactt ttcagcattt cttcgagcag attgttgagg
     1861 cgtaccgtac gcactgtctt acgcatgaaa
//
LOCUS       DBMDSQ010000374         1877 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_13384_length_1877_cov_1.206081, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000374 DBMDSQ010000000
VERSION     DBMDSQ010000374.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 1877)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1877)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1877
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13384_length_1877_cov_1.206081"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..267)
                     /locus_tag="ACZPAX_10190"
     CDS             complement(<1..267)
                     /locus_tag="ACZPAX_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005938948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR1114303.1"
                     /translation="MKKLISMALAAGMAVSLAACGGAASSSAPAADSAAASTTEAAAD
                     SDLAYIQGKGKMTIGYTVYEPMNYTDADGNFTGFDTELATAVCEK"
     gene            complement(436..1449)
                     /locus_tag="ACZPAX_10195"
     CDS             complement(436..1449)
                     /locus_tag="ACZPAX_10195"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015536924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HIR1114304.1"
                     /translation="MNCAELLQAARGQMGPCRACPVCNGRACGGSIPGPGAKGSGTVA
                     IRNFDAWRNVRLNMDTIHENFTPDTSLQLFGRTFKYPFFAGPVGAMTLHYGDKYNDMD
                     YNAILVPACAAAGIAAFTGDGTNPKVMEGATAAIAANGGFGIPTVKPWDNATVAKKMA
                     MARESGCFALAMDIDAAGLPFLQNLTPPAGSKTVEQLQEIAREAGVPFILKGIMTAKG
                     AEKAVQAGVAGIVVSNHGGRVLDDCPATEEVLPEIVEAVAGRAKVFVDGGIRTGGDVF
                     KALALGADAVLVARPYVTAVYGGGAEGVACLTEKLGAELADTMRLCGAAALKDISRDM
                     VRV"
     gene            complement(1506..>1877)
                     /locus_tag="ACZPAX_10200"
     CDS             complement(1506..>1877)
                     /locus_tag="ACZPAX_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017826695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HIR1114305.1"
                     /translation="LHLLDRLGLAEKAEAYPAQLSGGQKQRVAIARALAMNPDIMLFD
                     EPTSALDPEMVGEVLQVMKQLAADGMTMVVVTHEIGFAREVASRVIFMEGGYIVEEGT
                     PEEVIDHPKQPRTIDFLSKVL"
BASE COUNT          366 a          601 c          547 g          363 t
ORIGIN      
        1 cttttcacag acggcggtgg ccagctcggt gtcaaagccg gtaaagttgc cgtcggcgtc
       61 ggtgtagttc atcggctcgt agacggtgta accgatggtc atcttgccct taccctggat
      121 gtaggccagg tcgctgtcgg cagcggcctc ggtggtggaa gcagcggcgg aatcggctgc
      181 cggggcagac gacgaagcgg caccgccgca agcggccagg ctgaccgcca taccggcagc
      241 cagcgccatg ctgatgagtt ttttcatagt tgtgacctcc tcaaaatact ttgcacagtt
      301 ccctctcacg cttgctttat cgattcaggg tgataaagca agcgtatgct acaattttac
      361 cgcaaactgt aattttatgc aagtttaaaa tgcaaaaagg tggataaatc ggcagttaat
      421 gcgcccaaaa tgcaatcaga cccgtaccat atcccgggaa atatccttaa gtgcagccgc
      481 accgcacagc cgcatggtgt cagccagctc ggcccccagc ttctcggtca ggcaggcaac
      541 gccctcggca ccgccgccat acaccgccgt aacgtagggg cgggcaacca gcacggcgtc
      601 tgcgcccagc gccagcgcct tgaacacatc gccgccggtg cggatgccgc catccacaaa
      661 cactttggca cggcctgcca cggcttccac gatctccggc agcacttcct cggtggctgg
      721 gcaatcgtcc aacacgcggc cgccgtggtt agacaccacg atgcccgcca caccggcctg
      781 cacagccttt tcggcgccct tggcggtcat gatgcccttc aaaataaagg gaacgcccgc
      841 ctcccgagcg atctcctgca gctgctccac ggtcttgctg cccgccggcg gggtcaggtt
      901 ctgcaaaaag ggcaagcccg ctgcatcaat gtccatggcc agcgcaaagc agccggactc
      961 gcgtgccatc gccattttct ttgcaacggt ggcgttatcc cagggcttga ccgtggggat
     1021 gccgaagccg ccattggccg caatggccgc ggtggccccc tccatcacct tggggttggt
     1081 gccgtcaccg gtaaaggcgg caatgcccgc agccgcacag gcagggacca ggatggcatt
     1141 ataatccatg tcattgtact tgtcgccgta gtgcagcgtc atcgcgccca ccggcccggc
     1201 aaaaaatggg tatttaaatg tacgcccaaa cagctgcaag ctggtatcgg gggtaaaatt
     1261 ctcgtggatg gtgtccatat tcagccgcac attgcgccag gcgtcaaagt tgcggatggc
     1321 cacggtgccg ctgcccttgg cgccggggcc ggggatgctg ccgccgcagg cacggccgtt
     1381 gcacacgggg caggcgcggc aggggcccat ctgcccgcgg gcggcctgca atagttctgc
     1441 gcaattcatg attttgcctc ctttaaataa agggcctccg gctttgcgcc agaggccgca
     1501 gtcccttaca gaaccttgct caaaaagtcg atggtacgtg gttgtttcgg gtggtcgatg
     1561 acctcttcgg gggtgccctc ctccacgatg tagccgccct ccataaagat cacgcggctg
     1621 gctacctcgc gggcaaagcc gatctcatgg gtgacgacga ccatcgtcat gccgtccgcc
     1681 gccagctgtt tcataacctg caaaacctcg ccgaccatct ccgggtccag cgccgaggtc
     1741 ggctcgtcaa acagcatgat gtcggggttc atcgccagcg cacgggcaat cgccacacgc
     1801 tgcttctggc cgccggaaag ctgtgccggg taggcttcgg ccttttcggc cagccccaag
     1861 cggtccagca gatgcag
//
LOCUS       DBMDSQ010000375         1864 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_13521_length_1864_cov_1.766307, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000375 DBMDSQ010000000
VERSION     DBMDSQ010000375.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 1864)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1864)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1864
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13521_length_1864_cov_1.766307"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1078
                     /gene="ilvA"
                     /locus_tag="ACZPAX_10205"
     CDS             <1..1078
                     /gene="ilvA"
                     /locus_tag="ACZPAX_10205"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007045715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006567 - L-threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HIR1114306.1"
                     /translation="VTGSFKVRGAYYKISQLSAEERAKGVIACSAGNHAQGVALAATR
                     MGIKSVVCMPDGAPIMKVESTKRLGAEVELVKGTYDDAHDRAVELQEQTGMTFIHPYD
                     DELVIAGQGTIGLEILDQLPDVDAVIVPIGGGGLISGVAFAIKSLRPEVKIYGVQAAG
                     APSMEHAFHDHKYETLDSAVTFADGIAVKTPGETTFDMVSQYVDDIVTVSEDEIAAAI
                     LALMENQKLVAEGAGATPVAAALFGKLPLAGKKTVCLISGGNIDVNILSRVITRGLVM
                     SGRKTNLMIALEDKPGQLSLVSDIVSACGANVVSVHHDRSDANMAITSCFLKLGLETR
                     DAAQIETIREKLTEAGFKLVTERA"
     gene            complement(1167..>1864)
                     /locus_tag="ACZPAX_10210"
     CDS             complement(1167..>1864)
                     /locus_tag="ACZPAX_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007045716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR1114307.1"
                     /translation="VRYPDPQRLSELPGYEQEREKVIANTRALLEGRPANNVLLYGDA
                     GTGKSSTVKAIANEFAPDGLRLIEVKKNQLYQIPALMDELAKNPLKFILFIDDLSFAS
                     NDDNFAALKAILEGGVGGRSHNVAVYATSNRRHLVKETMADRSGDDLHAMDTRQELMS
                     LSARFGLTVTFQQPDKDRYDRILLELAKQYNVQMPSDQLFIKGAAFALRAGGRSPRVA
                     KQFVELLAAGVKV"
BASE COUNT          364 a          564 c          584 g          352 t
ORIGIN      
        1 agtcaccggc agcttcaagg tgcgcggcgc gtactacaaa atcagccaac tttcggcgga
       61 agaacgtgcc aagggcgtca tcgcctgctc ggcgggcaac cacgcacagg gcgtggcgct
      121 ggccgccacc cgcatgggca tcaagagcgt tgtctgtatg ccggacggtg cccctattat
      181 gaaagtggaa agcaccaagc ggctgggcgc agaggtcgag ctggtcaagg gcacctacga
      241 cgacgcccat gaccgcgccg tggaactgca ggagcagacc ggcatgacct tcatccaccc
      301 ctatgacgac gagctggtca ttgcggggca gggaaccatc ggcctggaga tcctcgacca
      361 gctgcccgat gtggacgcgg tcatcgtgcc catcggcggc ggcggtctga tttccggcgt
      421 ggcgtttgcc atcaagagcc tgcgtcccga ggtaaaaatc tacggcgtac aggcggcggg
      481 tgcccccagc atggagcacg cgttccacga tcacaagtac gagacgctgg actccgccgt
      541 gacctttgcc gacggcattg cggtcaagac gcccggtgag acgacctttg acatggtgag
      601 ccagtacgtg gacgacattg tcacggtcag tgaggacgag atcgctgccg ccatcctggc
      661 gctgatggag aaccagaagc tcgtggccga gggcgcaggt gccacgccgg tggctgccgc
      721 gctgttcggc aaactgccgc tggcgggcaa gaaaaccgtc tgcctgattt ccggcggcaa
      781 cattgatgtg aacattctct cccgcgttat cacccgcggc ctggtcatga gcggccgcaa
      841 gacgaacctg atgattgccc tggaggacaa gcccggccag ctgagcctgg tcagcgacat
      901 tgtctcggcc tgcggcgcaa acgtcgtcag cgtccaccac gaccgcagtg acgcgaacat
      961 ggccatcacc agctgtttcc tgaagctggg gctagagacc cgcgacgccg cgcagatcga
     1021 gacaatcagg gagaagctga ccgaggccgg atttaagctg gtgacagaga gggcgtaaac
     1081 gtaaactaag tcaaaagaaa aaagaaaaga gattttctct tgccagactc tcttctctcc
     1141 tctctcttat ttcttcgctg tcaaggtcag accttgaccc ccgctgccag caattccaca
     1201 aactgttttg ccacgcgggg actgcggccg ccggcacgca gagcgaaggc cgcgcccttg
     1261 atgaacagct ggtcgctggg catctggacg ttgtactgct tcgccagttc cagcaaaatg
     1321 cggtcgtagc ggtccttgtc gggctgctgg aacgtcaccg tcaggccgaa ccgggccgac
     1381 aggctcatga gttcctggcg agtgtccatc gcgtgcaggt cgtcgccgct gcggtcggcc
     1441 atcgtctcct tgaccaggtg gcggcggttg ctggttgcgt agaccgccac gttatggctg
     1501 cgcccgccga cgccaccctc cagaatcgct ttcagcgcgg caaagttatc gtcgttggac
     1561 gcaaagctca aatcgtcgat aaacaggatg aatttcagcg gatttttcgc cagttcgtcc
     1621 atcagggcgg gaatctggta cagctggttt ttcttcactt cgatcaggcg caggccgtcg
     1681 ggggcgaact cgttggcaat ggccttgacc gtgctggatt ttccggtgcc cgcatcgccg
     1741 tacagcaaaa cgttattggc ggggcggcct tccagcaggg cgcgggtgtt ggcgatgacc
     1801 ttctcccgct cctgctcgta gccgggcagt tcgctcaaac gctgcgggtc ggggtagcgc
     1861 accg
//
LOCUS       DBMDSQ010000376         1856 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_13598_length_1856_cov_1.060399, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000376 DBMDSQ010000000
VERSION     DBMDSQ010000376.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 1856)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1856)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1856
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13598_length_1856_cov_1.060399"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            241..783
                     /locus_tag="ACZPAX_10215"
     CDS             241..783
                     /locus_tag="ACZPAX_10215"
                     /inference="COORDINATES: protein motif:HMM:NF027438.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4830 domain-containing protein"
                     /protein_id="HIR1114308.1"
                     /translation="MFLFTVTKPGLRQAGALALCAVCLCGTVAAAVHFTGGAVTVSAA
                     AVPGIETTQDIGTYFTGYGFDTDLASAAVDKVKIPKKWDDSFTAFNQIVQESGLDLAD
                     YKGKTVEKWTVLCPQLSTGDHDTYCVLLVYKAKAVGAYLLQKPSGEVTGLVTAAKASA
                     DAALEEGETVEISADAFPED"
     gene            complement(938..>1856)
                     /locus_tag="ACZPAX_10220"
     CDS             complement(938..>1856)
                     /locus_tag="ACZPAX_10220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1114309.1"
                     /translation="VYFNGQNIWWLGLCVALLAAKDVPLRRVMKAFLASGVPTLALVQ
                     VLHFAGIIAPNAASERDGSYRLMFGYGHPNTFGGVVLGLLLAWVLLRRARLTWAELGG
                     LAAVGVFLMAAPKSRSSALCTCLLVLLLALAKLYTRKGPRPVGRLAAGGCAALVPLLA
                     AVSYILPLFVVKIGPWANDIGPGWLKKLDDLLTCRISLAWAAYRMFDIKIAGQFLQEW
                     PALDNIFVYLLYLTGPVMVVLVCALMMAALYGYARRGAWQTVACLVTMLAYGYMESQV
                     IHLTSDPAVLLLCGAVFALPRDKWMFEDA"
BASE COUNT          398 a          589 c          568 g          301 t
ORIGIN      
        1 gcagccttgg gcgctatgat ggtcatgtgg acggccctgc ccgagcatga caccgccaag
       61 ctggaagcgg agaacgacgc cgttatcttc cagtggtacg cgccaaagca gaaataccgg
      121 gagtaacccc tgtgccgtag gggcgcattc cataggcgcc cgcaattcat gcacgataac
      181 cgcataaacg ccgcagcccc cgcatacatt gcattgacaa tgtgtgaggg ggctgcgttt
      241 atgtttttat ttactgttac caaaccgggt ctgcggcagg ccggggcgct ggccctgtgc
      301 gcggtgtgcc tgtgcggcac ggtggctgcg gcggtgcatt ttaccggcgg ggcggtcacg
      361 gtgtcggctg cggcggtgcc gggcatcgag acgacccagg acatcggcac ctactttacc
      421 ggctacggct ttgacaccga ccttgccagt gccgccgtgg acaaagtgaa gattcccaaa
      481 aagtgggacg acagtttcac ggcgttcaac cagatcgtac aggaaagcgg cctggatctg
      541 gccgactaca agggtaaaac ggtggaaaag tggacggtgc tctgcccgca gctttccacc
      601 ggcgaccacg acacttactg cgtgctgctg gtctacaagg ccaaggccgt gggggcttac
      661 ctgctgcaaa agccgtcggg tgaagtgacg ggccttgtca ccgccgccaa agccagcgcc
      721 gacgccgcgt tggaggaagg ggagaccgtg gaaatttcag cagatgcgtt cccggaggat
      781 taaacaaaaa gccttccccc atcgggggaa ggtggccgag caaagcgagg ccggatgagg
      841 ggcgcgttcg tcttgctacc catttagagg gcaatattgg caatgccgcc cctcatcagt
      901 ccgcttcgcg gacagcttct ctcgcaggag aagcctttta cgcatcttca aacatccact
      961 tatccctcgg cagcgcgaac accgcaccgc acagcagcag cacggcggga tcgctggtca
     1021 ggtggatgac ctggctctcc atgtagccgt aggccagcat cgtgaccagg cacgccacag
     1081 tctgccacgc gccgcgccgg gcgtagccgt acagggccgc catcatcagt gcgcagacca
     1141 gaacgaccat gaccgggccg gtcaggtaga gcagatacac aaaaatattg tccagcgcgg
     1201 gccattcctg taagaattgg cccgcaattt ttatgtcgaa catccggtag gccgcccatg
     1261 ccagcgaaat gcggcaggtc agcagatcgt cgagcttttt cagccagccg gggccgatat
     1321 cgttcgccca cggcccgatt tttacgacga acagcggcag aatatagctg accgccgcca
     1381 gcaacggcac cagcgccgca cagccgccag ccgccagccg cccgaccggg cgcgggcctt
     1441 tgcgggtgta caacttggca agcgccagca gcaacaccaa taaacacgta cacagcgctg
     1501 acgaacggct cttgggcgct gccatcaaaa acacgcccac ggcggccaga ccgcccagct
     1561 cggcccaggt cagccgggca cggcgcagca gcacccaggc cagcagcagg ccgagcacca
     1621 ccccgccgaa ggtgttgggg tggccgtacc cgaacatgag ccggtaactg ccgtcgcgct
     1681 cactggcggc gttgggtgca atgatgcccg caaagtgtag cacctgcacc agtgccagcg
     1741 tgggcacgcc gctggccaaa aacgctttca tcacacggcg cagcggcaca tccttggcgg
     1801 ccagcagcgc cacgcacagg cccagccacc agatgttctg gccgttgaaa tagacc
//
LOCUS       DBMDSQ010000377         1842 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_13718_length_1842_cov_1.515796, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000377 DBMDSQ010000000
VERSION     DBMDSQ010000377.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 1842)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1842)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1842
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13718_length_1842_cov_1.515796"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..642)
                     /locus_tag="ACZPAX_10225"
     CDS             complement(<1..642)
                     /locus_tag="ACZPAX_10225"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR1114310.1"
                     /translation="MEHIAISVIVPIYNTKPYLEECIESILAQKINVPIEVLLIDDGS
                     TDGCAEICDKYAARDERVRVIHQENQGLSAARNAGIDNAKGRYYSFVDSDDIVLPRFL
                     QTLYDACEEHDAYMSLCSVEDVQEDGKSCDPAYFTRPTQEGIFCGKNLLNEFYTPYGT
                     VYTVAWNKLYRAEVWKLLHYPEGRLHEDDFVAHRLFWRCDKVVCVDKPLYHYRL"
     gene            complement(645..1661)
                     /locus_tag="ACZPAX_10230"
     CDS             complement(645..1661)
                     /locus_tag="ACZPAX_10230"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR1114311.1"
                     /translation="MSDHPKISIISTVYNTGPYLRPAVESILAQTFTDFELLLVDDGS
                     HDGSAEVCDALAAQDARIRVFHQANGGPAHARNTGLDNVRGDYIGIVDSDDLIEPTMF
                     ETLYNAVQAPGVRLAACAADCIDADGSKIEGRMVTIRQTGVRDAQELLLEAFQTGSFY
                     GPLSWNKLFDARLFKEKGIHYDETMLFGDDASVLHRVFEGERCNCLGDVLYHYRTRAG
                     QITTAGFPPRKIDDLRMYWEWLQYFSARPGREEYYDWAVVRYWQIFYQFWCQSDAAGN
                     LPELKPKFLAHKNHLDAILPDIIASKHLPLGEKLRAAAFCASPTLTYGAAAAWGRLHK
                     RKGN"
BASE COUNT          347 a          550 c          565 g          380 t
ORIGIN      
        1 caggcggtaa tggtagagcg gcttgtccac acagacgacc ttatcgcagc gccagaacag
       61 gcggtgcgcc acaaaatcat cctcatgcag gcggccttcg gggtagtgca gcagcttcca
      121 gacctcggcg cggtagagtt tgttccaggc cacggtgtag accgtgccgt agggggtgta
      181 gaactcattc agcagattct tcccacagaa aataccctcc tgcgtggggc gggtaaagta
      241 ggccgggtca cagctcttgc cgtcctcctg cacgtcctcg accgagcaga gggacatata
      301 ggcgtcgtgc tcctcgcagg cgtcgtacag cgtctgcaaa aagcgcggca gtacgatatc
      361 gtcggagtcg acgaaggaat agtagcggcc cttggcgtta tcaatgcccg cattgcgcgc
      421 agcggacagg ccctggttct cctggtgaat gacccgcacg cgctcgtccc gggcagcata
      481 tttatcgcag atttcggcgc agccgtcggt ggagccatcg tcgatgagca gcacctcgat
      541 gggcacattg atcttctggg ccaggatgct ctcgatgcac tcttccagat acggcttggt
      601 gttgtagatc ggcacgatca cgctgatcgc aatatgttcc atggttagtt ccccttcctc
      661 ttgtgcagac gtccccaggc cgccgccgcg ccgtaggtca gcgtggggct tgcgcagaag
      721 gctgcggccc gcagcttctc gcccagcggc agatgcttac tggctatgat atccggcaaa
      781 atggcgtcca gatgattttt gtgcgccaaa aatttaggct tcagttcggg caggttacct
      841 gccgcgtcgg attggcacca gaactggtaa aagatctgcc agtaccgcac aacggcccag
      901 tcgtagtatt cctcgcgccc cggccgggcc gagaaatact gcagccattc ccagtacatc
      961 cgcaggtcat cgattttgcg gggcgggaag cctgccgtcg tgatctgccc ggcgcgggtg
     1021 cggtaatggt agagcacgtc gcccaggcag ttgcagcgct caccttcaaa gacgcggtgc
     1081 agcacactgg catcgtcgcc aaacagcatc gtctcatcgt aatggatgcc tttttccttg
     1141 aagagccgcg cgtcaaacag cttgttccag ctcagcggcc catagaaact gccggtctgg
     1201 aatgcctcta acagcagttc ctgggcgtcg cgcacgccgg tctggcggat cgtgaccatc
     1261 cggccctcga tcttgctgcc gtcggcgtcg atgcagtccg ccgcacaggc cgccagccgc
     1321 acgccggggg cctgaactgc gttataaagc gtctcgaaca tcgtcggctc gatgaggtcg
     1381 tcggaatcca cgatgccgat gtagtcgccc cggacattgt ccagcccggt attgcgggcg
     1441 tgggcggggc caccgttggc ctggtggaat acccggatgc gggcatcctg cgcggccagt
     1501 gcgtcacaga cttccgccga gccgtcgtgg ctgccgtcgt cgacgagcag cagctcaaaa
     1561 tcggtgaagg tctgcgccag gatgctttcc actgcggggc gcagataagg ccctgtgttg
     1621 tacaccgtgg agattatgct gattttggga tggtcagaca taggtacttc cttttattgt
     1681 aactgccttg tgggggccgg gcatgcccgg cccggtcgtg taaggcgagc gggcgtttat
     1741 gggaaggcgg agggccgcat tctatatgcg cccgcattac agcagccgct gtttgatgag
     1801 cgtatacgcc gccaataacc acggccaatt ccatgccaca cc
//
LOCUS       DBMDSQ010000378         1835 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_13784_length_1835_cov_1.708766, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000378 DBMDSQ010000000
VERSION     DBMDSQ010000378.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 1835)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1835)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1835
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13784_length_1835_cov_1.708766"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..650)
                     /gene="mutL"
                     /locus_tag="ACZPAX_10235"
     CDS             complement(<1..650)
                     /gene="mutL"
                     /locus_tag="ACZPAX_10235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005925598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HIR1114312.1"
                     /translation="MAEIRVLDKHTAELIAAGEVVERPASVAKELLENAIDAGATQIT
                     LSATRGGIQQLQIVDNGSGIEAEYIDKAFIRHATSKIATADDLGHIHTLGFRGEALAS
                     IASVAKVEVLTKTEADEYACCYRIEGGEPQGMEPGARPVGTTITVNDLFYNTPARMKF
                     LKKDASEGTFVAETVLHAALSHPEISFRFLRDGKQQYVTPGDGDLRSAVYAVLGRA"
     gene            complement(666..>1835)
                     /gene="mutS"
                     /locus_tag="ACZPAX_10240"
     CDS             complement(666..>1835)
                     /gene="mutS"
                     /locus_tag="ACZPAX_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HIR1114313.1"
                     /translation="FGYYIEVSRSYTDSVPDTFTRKQTLTTGERYITPELKELENKIL
                     GANERLLVLEHQLFADLLSSISAEIVRIQRTASAVAQLDVLAGFAEAALQNNYVMPTV
                     DESGVMEIKEGRHPVIEQMLKGSLFVPNDTLLDEDENRMLLITGPNMAGKSTYMRQNA
                     LIALMAQIGSFVPAASAHIGVVDAIFTRVGASDDLAAGQSTFMVEMTEVAEILKNATK
                     DSLVILDEIGRGTSTFDGMSIARSVVEFICENIGCKTLFATHYHELTSMEQDIHGVKN
                     YNIAVKKRGEDITFLRRIVAGPADDSYGIEVAKLAGLPGSVTKRAHAVLRQLEANAPG
                     RNSTMQLDFDTVEAYNNPSVPSEVVEKLHTVDIETLTPLEALNFLYELKNTLDKD"
BASE COUNT          330 a          544 c          555 g          406 t
ORIGIN      
        1 aacgcgcggc ctaagaccgc gtagacagca ctgcgcaggt cgccgtcgcc gggggtgaca
       61 tactgctgct tgccgtcgcg cagaaagcgg aagctgatct ccgggtggga cagcgccgcg
      121 tgcagcaccg tctcggccac gaaggtgccc tcgctggcgt ccttcttcag aaatttcata
      181 cgggcggggg tgttgtaaaa caggtcgttg accgtgatgg tcgtgcccac cgggcgagca
      241 cccggctcca taccctgggg ctcgccgccc tcgatgcggt agcagcaggc atactcgtcg
      301 gcttcggtct tggtcagcac ctcgaccttg gcaacgctgg caatcgacgc cagcgcctcg
      361 ccgcggaagc ccagggtgtg gatgtgcccg aggtcgtccg ccgtagcaat cttgctggta
      421 gcatggcgga taaacgcctt atcgatatat tctgcctcga tacccgagcc attgtccacg
      481 atttgcagct gctggatgcc gccgcgcgtt gcagacagcg tgatctgcgt tgcacccgcg
      541 tcgatagcat tttccaaaag ttcctttgcc acggaggccg gccgctctac gacctcgcct
      601 gccgcgatca attcagcggt atgcttgtcc agcacacgaa tttccgccat gggtctcacc
      661 tgcctttagt ctttgtccag ggtatttttc agttcgtaca aaaagttcag cgcttccagc
      721 ggggtcagcg tctcgatatc gacggtgtgc aacttttcca ccacctcgct ggggacgctg
      781 gggttgttgt aggcttcgac ggtgtcaaag tccagctgca tcgtgctgtt gcggcctggg
      841 gcgttggcct caagctggcg cagcacggcg tgggcgcgtt tggtcacgct gcctggcagg
      901 cccgcgagtt tggcgacctc gatgccgtag ctgtcatccg ccgggcctgc aacgatgcgg
      961 cgcaaaaacg tgatatcctc accgcgcttt ttgacggcga tgttgtagtt tttgacgccg
     1021 tggatatcct gttccatact ggtcagctcg tggtaatggg tagcgaacag cgtcttgcag
     1081 ccgatgttct cacagataaa ttctaccacg ctgcgggcaa tactcatgcc gtcaaacgtc
     1141 gaagtgcccc ggccgatctc atcgagaatg accaggctgt ccttcgtcgc atttttgagg
     1201 atctccgcga cctcggtcat ttcgaccatg aaggtcgact gcccggcagc caaatcgtcc
     1261 gacgcgccga cgcgggtaaa aatcgcatcc accacgccga tgtgggcgct ggcggcgggt
     1321 acaaagctgc cgatttgcgc catcagggca atcagcgcat tctggcgcat gtaagtagat
     1381 ttaccggcca tgttcgggcc ggtgatgagc agcattcggt tctcgtcctc gtccaagagc
     1441 gtgtcgttcg gcacgaacag gctgccttta agcatctgct caatgaccgg gtggcggccc
     1501 tctttaatct ccatgacgcc gctctcatcg acggtgggca tgacgtagtt gttttggagt
     1561 gcggcctcgg caaagcctgc cagcacgtcc agctgcgcca cggcggacgc cgtgcgctgg
     1621 atgcggacga tctcggcaga aatcgacgag agcaggtcag cgaacagctg atgttcgagg
     1681 acgagcagcc gctcattcgc gccgaggatc ttattttcca gctctttcag ttcgggcgtg
     1741 atgtagcgtt cacccgtcgt cagcgtctgc ttgcgggtaa aagtatcggg tacggagtcc
     1801 gtgtaagagc ggctgacctc gatgtaatag ccgaa
//
LOCUS       DBMDSQ010000379         1822 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_13912_length_1822_cov_1.510169, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000379 DBMDSQ010000000
VERSION     DBMDSQ010000379.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 1822)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1822)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1822
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13912_length_1822_cov_1.510169"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(31..903)
                     /locus_tag="ACZPAX_10245"
     CDS             complement(31..903)
                     /locus_tag="ACZPAX_10245"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005926216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - (3S)-3-hydroxyacyl-CoA
                     dehydrogenase (NAD+) activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HIR1114314.1"
                     /translation="MKVGVIGAGTMGQGIAKAFAQVEGYTVALCDIKQEWAEGGKNKI
                     AAGYAKLVAKGKLDQAEVDRRMAAITAGLKEDLCADCDLIVEAAFEDMKVKQTTFGEL
                     DKICKPECVFASNTSSLSITEIGKGLSRPLVGMHFFNPADRMKLIEVIAGVNTPVETV
                     EAIKKISTEIGKSPVQVNEAAGFVVNRILIPMINEAAFIKMEGVSDIAGIDTAMKLGA
                     NHPMGPLELGDFVGLDICLAIMDVLYKETGDSKYRACPLIRKMVRGGNLGCKTGKGFY
                     VYNADRTKTPVDAL"
     gene            complement(995..1780)
                     /locus_tag="ACZPAX_10250"
     CDS             complement(995..1780)
                     /locus_tag="ACZPAX_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015538446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HIR1114315.1"
                     /translation="MSFVTTEIQDAVAVLTIDRPKALNALNPEVLADLKAAFEAIDQN
                     TVRCVVLTGAGDKSFVAGADIGSMSTMTKAEGEAFGKLGNDVFLMIEHFPLPVIAAVN
                     GFALGGGNELAMSCDIRLCSDNAVFGQPEVGLGITPGFGGTQRLARLVGMGMAKQLVY
                     SALNIKADEALRIGLVNAVYTQEELMPAALKLAGKIAKNAPIAVRNCKKAINDGISLP
                     IEKAVEVEEKLFGDCFETHDQVEGMGCFLSREKPKPKPVFTNN"
BASE COUNT          378 a          531 c          528 g          385 t
ORIGIN      
        1 ttgccgcgcc aggcgcgtaa gtttacgaaa ttacagagcg tcgaccgggg tcttggtgcg
       61 gtcagcgttg tagacgtaga agcccttgcc ggtcttgcag cccaggttgc cgccgcggac
      121 catcttgcgg atcagcgggc aggcacggta cttgctgtcg ccggtctcct tgtacagaac
      181 gtccatgatg gccaggcaga tatccagacc aacaaagtca cccagctcca gggggcccat
      241 cgggtggttg gcacccagct tcatggcggt gtcgataccg gcgatgtcgg agacaccctc
      301 catcttgatg aaggcggcct cgttgatcat cgggatcagg atgcggttga cgacgaagcc
      361 ggcagcctcg ttgacctgaa ctgggctctt gccgatctcg gtgctgatct tcttgatggc
      421 ctcaacggtc tcaacggggg tgttgacacc ggcgatgacc tcgatgagct tcatgcggtc
      481 agcagggttg aagaagtgca taccgaccag aggacgagac aggcccttgc caatctcggt
      541 gatggacaga gaagaggtgt tggaagcgaa aacgcactcg ggcttgcaga tcttatccag
      601 ctcgccaaag gtggtctgct tgaccttcat atcctcgaaa gcagcctcca caatcaggtc
      661 gcagtcagcg cacaggtcct ctttcaggcc agcggtgata gcagccatgc ggcggtcgac
      721 ttcagcctga tccagcttgc ccttggcaac cagcttggca tagccagcgg cgatcttgtt
      781 cttgccgccc tcggcccact cctgcttgat gtcacacaga gcaacggtgt agccctcgac
      841 ctgtgcgaaa gcctttgcaa taccctggcc catagtgcca gcgccaataa cgcctacctt
      901 catagtagtt acctccaatt taagataaaa gataatagat aaaagataaa aaacctgtgt
      961 gcgcgctgcg cacgatttta agatttatct aaaattagtt gttcgtgaaa accggcttcg
     1021 gtttcggctt ctcgcggctc aggaagcagc ccatgccctc gacctggtcg tgggtctcga
     1081 agcagtcgcc gaacagcttc tcctcgactt caacagcctt ctcgatgggc aggctgatac
     1141 catcgttgat agcctttttg cagttgcgca cggcgatggg agcgttcttg gcaatcttgc
     1201 cagccagctt cagcgcggcg ggcatcagtt cttcctgggt gtacacagcg ttcaccaggc
     1261 cgatgcgcag ggcttcgtcc gccttgatgt tcagggcaga gtagaccagc tgctttgcca
     1321 tgcccatgcc gacgagacgt gccaggcgct gggtgccgcc gaagcccggg gtgatgccca
     1381 ggcccacttc cggctggccg aacacggcgt tgtcggagca gaggcggata tcgcaactca
     1441 tggccagctc attgccgccg cccagcgcga agccgttgac agctgcgatg acaggcagcg
     1501 ggaaatgctc gatcatcagg aacacatcgt tgcccagctt gccgaaagcc tcgccctcgg
     1561 ccttggtcat agtggacatg gagccgatgt cagcaccggc aacgaagctc ttgtcgccag
     1621 cgccggtcag cacgacacag cggacggtgt tctgatcgat ggcctcaaag gcggccttca
     1681 ggtcagccag aacctccggg ttcagcgcgt tcagcgcctt cgggcggtcg atggtcagga
     1741 cagcaacagc gtcctgaatt tcggtagtaa caaaggacat ttttaattcc tccggtaatt
     1801 acatctcaac gatggtggcg ca
//
LOCUS       DBMDSQ010000380         1819 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_13946_length_1819_cov_1.456345, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000380 DBMDSQ010000000
VERSION     DBMDSQ010000380.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 1819)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1819)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1819
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13946_length_1819_cov_1.456345"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(32..1105)
                     /locus_tag="ACZPAX_10255"
     CDS             complement(32..1105)
                     /locus_tag="ACZPAX_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007712846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HIR1114316.1"
                     /translation="MKSGVSLQQMLTEVKRQSESKEDYLIAPNRLRMESYGKEMFLHL
                     SDDSGTELIEPMTITGIAHRQIGTHLRIPAAYYDRMREERPDLLAYNANTWFKQENSQ
                     RMLRTLDGSARAYLSNRYRRIDNIDIAGVTLPILGGLPDIRFESCQITESRMYIKAVN
                     PRLQAEVSPGDIVQAGVIISNSEVGLGSVSIQPLIYRLVCSNGMVVNEAAARRNHVGR
                     VTDSEENFSVYSQATLDAEDKAFVMKIQDTVRAAVEEARFAQVVGKMQETKAAQMDTR
                     DVPAVVKLASKEFHITDSESTGVLQRLIESNDLTLYGLSNAVTRHSQDVESYDRASEL
                     ESIGYSILTMPTQQWSRINQAAA"
     gene            complement(1121..>1819)
                     /locus_tag="ACZPAX_10260"
     CDS             complement(1121..>1819)
                     /locus_tag="ACZPAX_10260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1114317.1"
                     /translation="CCKDASCRRCSIWELYVLHHLRSSLPAVPKKKAKAPRRTKFVVP
                     DIEDEPLSDILLLHVNQRVTAALRTADSNITWEWATKDPLAIVKEGGRCLIRVHGLDG
                     FERAEVVLDKQAHLRCELINTVPLRPNPTADTPEKPRIPAETPPLPDPRIWYETEGRA
                     TLEGIVADLSSRGHHSMTVQEDGHIQLTDGSTSVLPELHGFPPVGCWEKLKQVFAGDG
                     LAAEETSGKLVLSW"
BASE COUNT          346 a          517 c          532 g          424 t
ORIGIN      
        1 cataaaaaat cctcctatat gtagggcagc atcaagcagc tgcctggttg attctcgacc
       61 actgctgtgt gggcatggtc aaaatgctgt aaccgatact ttccaattca ctggcgcggt
      121 catagctctc cacatcctgg ctgtgtcggg tcacggcgtt ggataaacca tacagcgtca
      181 aatcgttgga ttcgatcagc cgctgcaata cgccagtgct ttcgctgtcg gtaatgtgga
      241 actctttgct ggcaagtttg acgacggccg gcacatcacg ggtatccatc tgtgcggctt
      301 tggtctcctg cattttgccg acgacctgtg cgaaccgcgc ttcttccacg gcagcgcgca
      361 cggtgtcctg aatcttcatg acaaaggctt tgtcctccgc gtccaaagtt gcttgggagt
      421 agacgctgaa attttcctcg ctatctgtaa cacgccccac atggttacgg cgggcggcgg
      481 cttcattcac gaccatgccg ttgctgcaca ccagccgata gatcagcggc tgtatgctga
      541 cggaaccaag tccgacctcg ctgttgctga tgatgacccc tgcctgcaca atgtcgccgg
      601 gggatacctc cgcctgcaag cgggggttca cggccttgat gtacatacgg ctttcggtga
      661 tttggcagct ttcaaagcgg atgtcaggca gcccgccgag aatcggaagc gtcactccgg
      721 caatgtcgat attgtcgatg cggcggtagc gattgctcag gtacgcacgg gcggagccgt
      781 ccaaggtgcg cagcatacgc tggctgtttt cttgcttgaa ccatgtgttg gcgttgtagg
      841 ccagcaggtc ggggcgttcc tcccgcatac ggtcatagta ggcggcgggg atgcgcagat
      901 gagtgccgat ctgccgatgg gcgatgccgg tgatggtcat gggctcgatt agttcagtgc
      961 cgctgtcgtc cgacaggtgc aaaaacattt ctttgccgta ggattccatc cgcaggcgat
     1021 tgggggcaat gaggtagtcc tccttggact cgctctgccg tttcacttcg gtcagcatct
     1081 gctgtaagga tacaccggat ttcatacgcg aaacctccct ttaccacgag agaaccagct
     1141 tgccggaagt ttcctcggcg gccagcccat ctcctgcaaa gacctgtttc aacttttccc
     1201 agcagcccac aggtgggaaa ccgtgcagtt ccgggagtac cgatgtgctg ccatccgtaa
     1261 gctggatgtg accgtcctcc tgcacggtca tcgagtgatg cccgcgggaa ctgaggtcgg
     1321 ccactatgcc ctccagcgtt gcgcgtccct cagtttcata ccaaatgcgc gggtcgggca
     1381 gtgggggagt ctcagcggga atacgcggtt tttcaggcgt gtctgccgtg ggatttgggc
     1441 gcagcggcac ggtgttgatc aactcgcacc gcaagtgagc ctgcttgtcc aaaacgacct
     1501 cggcacgctc aaagccgtcc aatccgtgaa cacgaatcag gcagcgccct ccctctttga
     1561 cgatagcaag cggatctttg gtggcccatt cccatgtgat gttgctgtcc gccgtgcgaa
     1621 gcgcagcggt gacgcgttgg ttcacatgaa gcagcagaat gtcgctgagc ggttcgtcct
     1681 caatgtccgg caccacaaac ttggtgcggc ggggtgcctt tgctttcttc ttggggactg
     1741 ccggtaggct gctgcgcaga tggtgcagca catacagttc ccagatggaa cagcgcctgc
     1801 aggaggcgtc cttacagca
//
LOCUS       DBMDSQ010000381         1808 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_14059_length_1808_cov_1.780316, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000381 DBMDSQ010000000
VERSION     DBMDSQ010000381.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 1808)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1808)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1808
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14059_length_1808_cov_1.780316"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..471
                     /locus_tag="ACZPAX_10265"
     CDS             <1..471
                     /locus_tag="ACZPAX_10265"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HIR1114318.1"
                     /translation="EKLAALGYKGILFDIDNTLVHHGDDSTPEVDALFRHIHSLGLKT
                     LLLSDNSAARIERFNRNIRTLFIAEAGKPDPAAYRRACAMLGLPPEQVVCVGDQVFRD
                     IRGANSAGLDSILVDFIRLPGETHYGKKRVLEKAILWFYHRDPRRRGRLDGIGK"
     gene            481..1290
                     /locus_tag="ACZPAX_10270"
     CDS             481..1290
                     /locus_tag="ACZPAX_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019680386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HIR1114319.1"
                     /translation="MFCDISPTCYKIALQKEICKRHIKDFFAQENYADTQDTAPLPCI
                     VAQYSSHLIKRGKGIDPVLQENKAVNIRLANEKLNGILIRPGETFSFWHRVGKTTKRK
                     GYRDGRILVRNHILPGIGGGLCNLANTIHRVVLVSPLTVTEFHKHSDALAPDEGPRVP
                     FSSGTSVFYNNGDYRFKNETDQTFQLLLWCDEDNLYAELRCERPLPCRYRIVEEGHHF
                     QKEGGKYYRVSKIYKETLDAQTGQLLHRELVLDNHSEVMYDPALIPGVEKL"
     gene            1303..>1808
                     /locus_tag="ACZPAX_10275"
     CDS             1303..>1808
                     /locus_tag="ACZPAX_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HIR1114320.1"
                     /translation="MYAKVTSLGVTGLAGYIVQVEADLSGGLPQFNLVGLPDSAVKES
                     SERVRSAIKNLHYEWPASRITINLAPADVRKTGPVYDLPVFVGILAAQGKLPVPGGER
                     AFLGELGLDGTLRPVTGVLPMAMAAVQNGVKELFLPAENAAEAAVAEGLTVYPARSAQ
                     DVVLHLCGA"
BASE COUNT          361 a          551 c          560 g          336 t
ORIGIN      
        1 gaaaagctgg ccgcgctggg ctacaaaggc attttgtttg acattgacaa cactcttgtc
       61 caccacggcg acgattccac gcccgaggtg gacgcgctgt tccgccatat ccacagcctg
      121 gggctgaaaa cactgctgct ctccgacaat tctgccgcgc gcatcgagcg attcaaccgc
      181 aatatccgca cgctttttat tgccgaggcg ggcaagcccg accccgctgc ctaccgccgg
      241 gcctgcgcca tgctgggcct gccgcccgag caggtcgtct gcgtcggcga ccaggtgttc
      301 cgggatatcc gcggcgcgaa cagcgccggg ctggacagca tcctagtgga tttcatccgg
      361 ctgcccggcg agacgcacta tggcaaaaag cgtgtgctgg aaaaagccat cctctggttt
      421 taccaccgtg acccgcgccg ccggggccgt ctggacggca taggaaagtg aggcagtttc
      481 atgttctgcg atatcagccc gacctgttac aaaattgcgc tgcaaaagga aatctgcaag
      541 cgccatatca aggatttttt cgcacaggaa aactatgccg acacgcagga taccgcgcct
      601 ctgccctgca tagtggcgca gtacagttcg cacctcatca agcgcggcaa gggcatcgac
      661 ccagtcttgc aggagaacaa ggccgtcaac atccgccttg ccaatgaaaa gctcaacggc
      721 attctcatcc gcccgggcga gacgttttcc ttctggcatc gggtgggcaa gacgaccaag
      781 cgcaagggct accgggatgg gcggattttg gtgcgtaatc atattctgcc gggcatcggc
      841 ggcgggctgt gcaacctggc aaacacgatt caccgcgttg tgctggtaag ccctttgacc
      901 gtgaccgagt tccacaagca ctccgacgcg ctggcccccg acgaggggcc gcgggtgccg
      961 ttcagttccg gcacgtcggt gttttacaat aacggcgact accgttttaa aaatgagacg
     1021 gaccagacgt tccagctgct gttgtggtgc gatgaggaca acctctacgc cgagctgcgc
     1081 tgtgagcgcc ccctgccctg ccgctaccgc attgtggagg aagggcacca cttccaaaaa
     1141 gagggcggta aatattaccg tgtgtcgaag atttacaagg agacactgga cgcacagacc
     1201 gggcagctgc tgcaccggga gcttgtgctg gacaaccatt ccgaagtcat gtatgacccc
     1261 gcgctgattc ccggcgtgga aaaactgtaa agggagccgc ctatgtatgc aaaagtgaca
     1321 agcctcggcg tgaccgggtt agccgggtac atcgtgcagg tggaggccga tctgtccggc
     1381 gggctgccgc agttcaatct ggtgggcctg ccggattcgg cggtgaagga gagcagcgag
     1441 cgggtgcgca gcgccatcaa gaacctgcac tacgagtggc ccgccagccg tattaccatc
     1501 aacctggccc ccgccgacgt gcgcaagacc gggccggtat atgacctgcc ggtgttcgtg
     1561 ggcattctgg ccgcgcaggg caagctgccc gtccccggcg gcgaacgggc gtttttaggg
     1621 gagctgggcc tggacggcac attgcgcccc gtgaccggcg ttctgcccat ggcgatggcc
     1681 gctgtgcaga acggcgtgaa ggagctgttt ctgcccgccg agaacgctgc cgaagccgct
     1741 gtggccgaag ggctgaccgt ctaccctgcc cgcagtgccc aggatgttgt gctgcacctg
     1801 tgcggcgc
//
LOCUS       DBMDSQ010000382         1803 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_14115_length_1803_cov_1.506463, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000382 DBMDSQ010000000
VERSION     DBMDSQ010000382.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 1803)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1803)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1803
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14115_length_1803_cov_1.506463"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1214)
                     /locus_tag="ACZPAX_10280"
     CDS             complement(<1..1214)
                     /locus_tag="ACZPAX_10280"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HIR1114321.1"
                     /translation="MKKFCAILFSFVLVLIIALPVAAEGESSTLADNAPSLTAPAAYV
                     VNLDTNIVVYEKNSETPLSAASLTKLMTTLLLLENYQDQLDSISLTAPSYVYDLIWEQ
                     STNASSADIRRGETQSLRNLLYAMLLPSGNEAAYIVADYMGGGSIDNFVAMMNDEAKA
                     VGCTGTTFVDPCGLNPNNITTARDAYLILRALTAYDVFSTVVGTPSYDMGTNDRYTTP
                     GTYIIQTTDKLITNSSYHRDYTKGGKTGSLGEWQNFAGWHSQDGESYISILLNVPYDA
                     DPEGMRPALVETATIMDWVFDTYTIAPALDTTQPITEVRVAYSTQADTVMLYPADNMM
                     TLLPRDGGAALTEQVFNVPDQLPAPIKQGDIVGTVTLTIEGETIGTADLIAGSDVSRN
                     QLLYTISRVSLF"
     gene            complement(1480..>1803)
                     /locus_tag="ACZPAX_10285"
     CDS             complement(1480..>1803)
                     /locus_tag="ACZPAX_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015565873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HIR1114322.1"
                     /translation="DSCCILISDNGNDAVRERLQFLCRTSDGFAVAKYDLETRGPGDF
                     FGSAQHGLPTLRVADLVQDTRTLRVAQDEAKALLAEDPNLIDPAHRALSDEVERLFET
                     AGAMN"
BASE COUNT          326 a          512 c          582 g          383 t
ORIGIN      
        1 aaaaaaaggc tcacccggga aatcgtgtac aaaagctgat tgcgggaaac atcgctgccg
       61 gcgatcaggt cggcggtgcc gatggtctcc ccttctatgg tcagggtcac ggtgcccaca
      121 atgtccccct gcttgatggg cgcgggaagc tgatccggca cattgaacac ctgttcggtc
      181 agagctgcgc cgccatccct gggcagcagg gtcatcatat tgtcagccgg gtagagcatg
      241 acggtgtcgg cctgggtcga ataggccacc cgcacttcgg tgatgggctg ggtcgtgtcc
      301 agcgccgggg caatggtgta ggtatcgaac acccagtcca tgatggtagc tgtctccacc
      361 agcgcggggc gcatcccctc ggggtcagcg tcgtagggca cgttcagcag gatgctgatg
      421 tagctctcgc cgtcctggct gtgccacccg gcaaagttct gccactcgcc caggctgccg
      481 gtcttgccgc ctttggtgta gtcgcggtgg tagctgctgt tggtgatgag cttgtcggtg
      541 gtctggatga tataagtccc gggggtcgtg tagcggtcgt tcgtgcccat atcgtagctg
      601 ggcgtgccga cgaccgtgga gaacacatcg taggcggtca gcgcccgcag aatcaggtag
      661 gcgtcccgcg cggtggtgat gttgttgggg ttcagcccgc aggggtcaac aaacgtcgtg
      721 ccggtgcagc ccacggcctt agcctcgtcg ttcatcatgg cgacaaagtt gtcaatgctg
      781 ccgccgccca tgtagtccgc cacgatgtag gcggcctcgt tgccgctggg cagcagcatg
      841 gcgtagagca ggttgcgcag gctctgcgtc tcgccccggc ggatatcggc cgaggatgcg
      901 ttggtgctct gctcccaaat caggtcgtag acatagctcg gggcggtcag gctgatggag
      961 tcgagctgat cctggtaatt ttccagcagc agcagcgtcg tcatcagctt ggtcaggctg
     1021 gcggcggaga gcggcgtctc gctgtttttc tcgtacacga cgatgttggt gtcgaggttg
     1081 accacatagg cagccggggc ggtcagcgag ggagcattgt ccgcaagggt gctgctctcc
     1141 ccttctgccg cgaccggcag cgctatgatc agcacgagca cgaaggaaaa tagtatcgcg
     1201 cagaattttt tcataggcaa aggtttcctt tgtaaagtta cctatcagta taccaaactt
     1261 tgcggggaaa taaaagggat atgcaggatt ttttggcggt gggagtggtg ttttcgggtg
     1321 cgctgccttc tcccttgggg gaaggtgtct gcggctccga ccgcagacgg atgaggggca
     1381 gcggttccat tattgcccat acatgggcgg ccgcagtaag tctgtcccct catcagtcac
     1441 cttcggtgac agcttccccc tcaggggaag ccaaaggcct cagttcatcg ctcctgcggt
     1501 ctcaaacagc ctctccacct catcggacag tgcccggtgc gccgggtcga tcaaattcgg
     1561 gtcctcggcc aataacgctt tggcttcatc ctgcgctacc cgcagcgtgc gggtgtcctg
     1621 cacaaggtca gccacccgca gcgtcggcag gccgtgctgg gcggagccga agaaatcacc
     1681 agggccgcgg gtttcaaggt cgtactttgc cacggcgaag ccgtccgacg tgcggcacaa
     1741 aaattgcagc cgctcccgca cggcatcgtt gccgttgtcg gaaatcaaaa tgcagcagga
     1801 atc
//
LOCUS       DBMDSQ010000383         1801 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_14148_length_1801_cov_0.872353, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000383 DBMDSQ010000000
VERSION     DBMDSQ010000383.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 1801)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1801)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1801
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14148_length_1801_cov_0.872353"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="ACZPAX_10290"
     CDS             <1..250
                     /locus_tag="ACZPAX_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004066074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1114323.1"
                     /translation="AMTLENISNIDGLFAVINQCTGNVELISDEGDCINLKSRLAQYM
                     TVAGAFSDGYIRSLRLRVEKDEDKVRIFDFILSGEAEK"
     assembly_gap    382..391
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            393..1322
                     /locus_tag="ACZPAX_10295"
     CDS             393..1322
                     /locus_tag="ACZPAX_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009987013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1848 domain-containing protein"
                     /protein_id="HIR1114324.1"
                     /translation="MQTGFRTDIPAFYSEWFANRLRAGYVLVRNPYNPQAVTRYELNP
                     DMVDLIGFCTKNPAPMLQKMDLLRSYGQYWFVTITPYGPEIEPHVPPKETVLCDFVAL
                     SEIVGPDSIGWRYDPIFLSDTYTVERHIQEFARMAAVLAGHTHTCVISFIDLYEKVRR
                     NFPQVQGVSRADRLTLGKAFVEIGRQYGLTIRPCAEGDELAPYGADCSGCMTQQIFET
                     ALHRRLHLPPGKNSRKECACFMTADIGAYNTCGHLCRYCYANYSKELVTQNMRQHDPQ
                     SPFLIGHSLPGDVIHQAQQESWVENQLSLFDLL"
     gene            1450..>1801
                     /locus_tag="ACZPAX_10300"
     CDS             1450..>1801
                     /locus_tag="ACZPAX_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019680371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HIR1114325.1"
                     /translation="MDFAQVIKDRYSCKKYDGKPVTAEQLSSILEAGRLAPTAKNLQE
                     QHIYVVQSAEGLAKIDKLTPCRYGAPTVLVVAFNKDSVFTYPGGKHSSGAEDAAIVAT
                     HLMLAATNAAVDSCW"
BASE COUNT          392 a          505 c          495 g          399 t
ORIGIN      
        1 cgctatgact ctggaaaaca tctccaacat cgacgggctg ttcgccgtca tcaatcagtg
       61 caccggcaac gtggaactga tctctgacga gggcgactgc atcaacctga agtcccgcct
      121 ggcccagtac atgaccgtgg ccggtgcttt ctccgatggt tacatccgca gcctacgcct
      181 gcgcgttgag aaagacgagg acaaggtccg catctttgac ttcatcctca gcggtgaggc
      241 cgagaagtaa ttttcgccat tctctccata cgttctatat cgcaaaggcc gccgtgtcct
      301 gctcccccag caggctgcgg cggtctttgt cttttctcgt aaaaaatgta taggggcata
      361 atttggtttt taaaagtata tnnnnnnnnn ntttgcagac agggtttcgc acggatatcc
      421 cggcatttta cagtgagtgg tttgccaacc ggctgcgggc cgggtatgtg ctggtacgca
      481 acccctacaa tccccaggct gtgacgcggt acgagctgaa cccggacatg gtggatttga
      541 tcggcttctg caccaaaaat cccgcgccga tgctgcagaa gatggatctg ctgcgttcct
      601 atgggcagta ctggttcgtc accatcacac cctatgggcc tgagattgag ccgcatgtgc
      661 cgccgaaaga gaccgtactg tgtgattttg ttgccttatc cgagattgtc ggcccggaca
      721 gcatcggctg gcggtatgac ccgatttttc tttccgacac ctatacggtt gaacgccaca
      781 ttcaggagtt cgcccgcatg gcagcagtgc tggccggaca cacccacacc tgcgttatca
      841 gctttattga tctgtacgag aaagtgcggc gcaattttcc gcaggtgcag ggtgtctccc
      901 gtgccgaccg gctcacgctg ggcaaggctt ttgtggagat tgggcggcag tacggcctta
      961 cgatacgtcc ctgtgccgag ggagacgaac tggcccctta tggcgcggat tgcagcggct
     1021 gcatgaccca gcagattttt gaaaccgcgc tgcatcgtcg cctgcacctg ccgccgggta
     1081 aaaactcccg caaagagtgc gcctgcttta tgaccgctga catcggcgca tacaacacct
     1141 gcggccacct gtgccgctac tgctacgcca actacagcaa ggagctggtg acgcagaata
     1201 tgcggcagca tgacccacag tctccttttt taatagggca cagtctgccg ggcgatgtca
     1261 tccaccaggc gcagcaggaa agctgggtgg agaatcagct ttcgctgttt gatcttttat
     1321 aaaaccggag tgaagaacag gtttctgcat acacagcggg cttagcccat agagggctgg
     1381 aaattttgcg gcaaaaagta tatacttatg gtatggaatg tccgcaaaaa tgtaaggggg
     1441 aattctacca tggattttgc acaggtgatc aaagaccgct attcctgcaa gaagtacgat
     1501 ggcaagcctg tcacagcaga gcaactgagc agcattctgg aggctggccg cctggccccc
     1561 accgccaaga acctgcagga gcagcatatt tacgttgtcc agtccgccga ggggctggcc
     1621 aaaattgaca agctcactcc ctgccgctat ggcgcgccca cagtgctggt agtggccttt
     1681 aacaaggaca gcgtgttcac ctaccccggc ggcaagcaca gttccggcgc tgaggatgcc
     1741 gccatcgtgg ctacccacct gatgctggcc gccaccaatg ccgcggtaga tagctgctgg
     1801 a
//
LOCUS       DBMDSQ010000384         1787 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_14274_length_1787_cov_1.320878, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000384 DBMDSQ010000000
VERSION     DBMDSQ010000384.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 1787)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1787)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1787
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14274_length_1787_cov_1.320878"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..769
                     /locus_tag="ACZPAX_10305"
     CDS             <1..769
                     /locus_tag="ACZPAX_10305"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR1114326.1"
                     /translation="TDGTADYLKTLDMPGLVCRNPGKNLGGAGGFAYGIREAAALGCK
                     AVWIMDDDTLPTPTALETLLAADAAHGGDYGWLSGRALAPDGADKPMNLQRKTMYRDI
                     DGFGDGKGEIPAVMASFVSLFLRIETVRKFGLPIAEFFIWSDDWEYTRRISREKSCYV
                     VPSSRVVHAMQNPGVVNIAKDVPARWARYRYFYRNDVVLYRREGVIGWLWLLAKDAWH
                     TVQVLRDGQGRRAERIKIIWKGFAAGVKFHPQTPYLP"
     gene            766..>1787
                     /locus_tag="ACZPAX_10310"
     CDS             766..>1787
                     /locus_tag="ACZPAX_10310"
                     /inference="COORDINATES: protein motif:HMM:NF012905.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR1114327.1"
                     /translation="MNLTEHICHMAARYDAVAFDVFDTLIRRDTAAPTNLFRLRGETF
                     YTARTRAEREARAAKSGEVTLAEIYARPCLAGYDPAQECAAELAACTAYAPVQAAVQK
                     LAQQGKKLYYISDMYLPQTQINAMLRRCGYAQFAGGFVSCTYGVQKRSGRLFKQFLHE
                     TELDARQVLFIGDSWRADVAGAALAGIASWHLPTGPAPASDLDAFIQNRLQLCKTAGE
                     ALGISVLGPLATAYCRWLHERRAARPDAKLYFLARDMYLMRAVYAALYPDEETEYLRV
                     SRRSLAPAFLAAGDYASVRAALPRQSLTGAQIAAYCAAVCPPELAVQKFDLKAPDTAE
                     LDIFLQ"
BASE COUNT          328 a          557 c          565 g          337 t
ORIGIN      
        1 caccgacggt accgctgact acctgaaaac gctggatatg cctgggcttg tctgccgtaa
       61 tccgggcaaa aatctcggcg gtgcgggcgg ctttgcctac ggcattcggg aggctgcggc
      121 cctcggctgc aaggcagtct ggatcatgga cgacgataca ctcccgacgc ccacggcgct
      181 ggaaaccctg ctggcggcgg acgctgccca cggtggggac tacggctggc tgtctggtcg
      241 cgccctggcc ccggatggtg ccgacaagcc tatgaacctc cagcgcaaga cgatgtaccg
      301 cgatatcgac ggtttcggcg atggcaaagg cgagataccc gccgtgatgg cgagctttgt
      361 ctcgctgttt ctgcgcatcg agaccgtgcg gaagttcggc ctgcccatcg ccgagttttt
      421 catctggtcc gacgattggg agtacacccg ccgcatttcc cgcgaaaagt cctgctatgt
      481 cgtgccatcc agtcgggtcg tgcacgccat gcagaacccc ggcgtggtga atatcgcgaa
      541 ggacgtgccc gcccgctggg ccagataccg ctacttttac cgcaatgatg tcgtgctcta
      601 ccggcgggaa ggagtcatcg gctggctgtg gctgctggca aaggatgcct ggcacaccgt
      661 gcaggttttg cgcgacggcc agggccgccg ggccgaacgc atcaaaatca tctggaaagg
      721 ctttgctgcg ggggtcaagt tccacccgca gacaccgtac ctgccatgaa tctaacggaa
      781 cacatctgcc atatggccgc ccggtatgac gcggtcgctt tcgacgtgtt tgacacgctc
      841 atcaggcgtg acactgccgc gccgacgaat cttttccgcc tgcgtggcga gacgttttat
      901 accgcccgca cccgggccga gcgcgaagcc cgcgccgcca aaagcgggga agtaacgctg
      961 gcggaaatct acgcccggcc ctgccttgcg ggttatgacc ccgcgcagga gtgcgctgcc
     1021 gaactggccg cctgcacggc ttacgcaccg gtgcaggccg ctgtgcagaa attggcgcag
     1081 cagggcaaaa aactctacta tatttccgat atgtacctgc cgcagacgca gatcaacgcc
     1141 atgctgcgcc gctgcggcta cgcccagttt gcggggggct tcgtctcctg cacctacggt
     1201 gtgcagaagc gcagcggcag actgttcaaa cagtttttgc acgagacgga actggacgcc
     1261 aggcaggtgc ttttcatcgg ggacagctgg cgcgccgatg tggccggggc cgcgctggca
     1321 gggatagctt cgtggcatct gcccacaggc cccgccccgg cgtctgacct cgatgccttt
     1381 atacaaaacc ggttacaact gtgcaaaacg gcgggggagg cgctgggcat ttcggtgctg
     1441 gggccgctgg ctacggccta ctgccgctgg ctgcatgaac gccgtgccgc gcgcccggac
     1501 gcgaaactct atttcctcgc gcgggatatg tacctgatgc gcgcagtata cgccgcactg
     1561 tacccggacg aagagaccga atatttgcgg gtctcccgcc gcagcttggc cccggcgttt
     1621 ttggcggcgg gggattatgc cagtgtgagg gcggcactgc cacggcaaag cctgaccggg
     1681 gcgcagatcg ccgcctactg cgctgcagtc tgcccgccgg agctggctgt acaaaaattt
     1741 gacttgaaag ctccggacac tgcagaactg gatatctttt tgcaaaa
//
LOCUS       DBMDSQ010000385         1786 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_14284_length_1786_cov_1.517507, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000385 DBMDSQ010000000
VERSION     DBMDSQ010000385.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 1786)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1786)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1786
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14284_length_1786_cov_1.517507"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..517)
                     /locus_tag="ACZPAX_10315"
     CDS             complement(<1..517)
                     /locus_tag="ACZPAX_10315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009326006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HIR1114328.1"
                     /translation="MEKYVIRGGKPLVGDVNIGGAKNAAVAILPATILAGGKCLIENL
                     PCISDVMASLQILSELGASIRMVSRSTYEIDTTHIDSTEVSNELSRQMRASYYFLGAL
                     LGRFGAGQVAMPGGCNLGPRPIDQHLKAFNAFGAEDSVEYGQIHVKSNHLVGGHVFFD
                     TVSVGATMNAML"
     gene            complement(701..>1786)
                     /locus_tag="ACZPAX_10320"
     CDS             complement(701..>1786)
                     /locus_tag="ACZPAX_10320"
                     /inference="COORDINATES: protein motif:HMM:NF013700.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HIR1114329.1"
                     /translation="MTESEIANAILRTASDEIINGTAVYVDDQLLYVTTEGDHLRTYL
                     ENIKAPFEDAMDSSIYTAFAHSIRLVDGVYLQESVSSYADVISALSEGGGIHTYTAVE
                     GDTVQSVIDTTGVNFDSLAQMNPDLLSLDQEIPEGTVITTGASSAELIKVKVVRRDTE
                     TVAIPFDTQNSESDQYDFGKVVTVQEGVDGSEEITYETTMIDGAVTDRQAVAYNVLQA
                     PTPEITVTGTKLKNGMVAKLGSGSFIWPVPGYKYVSRWMGNGHRGADICAAYHTTIIA
                     SDSGTVVAAGWHYSYGNYVEIDHGNGYRTLYGHMSEIAVTQGQAVSQGQTIGYVGSTG
                     NSTGNHCHFEMSYNGALFSAYTLFPGM"
BASE COUNT          371 a          488 c          524 g          403 t
ORIGIN      
        1 ccaacatagc gttcatcgtc gcgccgacgg agactgtgtc aaaaaacaca tggccgccga
       61 caaggtgatt cgacttgaca tggatttgac cgtactccac cgaatcctcg gccccgaagg
      121 cgttgaatgc cttcaagtgt tggtcgatgg ggcgcggacc caggttacat ccgcctggca
      181 tggcgacctg ccctgcccca aagcggccca gcagtgcgcc aaggaagtag tagctggcac
      241 gcatctggcg ggacaactca ttggagactt cggtgctgtc aatatgcgtc gtatcgatct
      301 cataggtgct gcggctgacc atgcggatgc tggcacctag ctcgctcaat atctgcagcg
      361 acgccataac gtcgctgatg cagggaagat tttcaatcag gcatttcccg cccgctaaaa
      421 tcgtggcagg cagtatggca actgcggcgt ttttggcacc gccaatgttg acatcgccga
      481 ccagcggctt gcccccacga ataacgtatt tttccaaatg tagctctcct ttgtgcagcg
      541 gttcgccgtc aaccctctgt cagacagcgt tccaatagta cacactttac atgattatac
      601 aacattttgc ggtgattgaa aagtcctgtt ctacctattt gcaaaatttt tgtgaaacag
      661 tacaagatgt gcttgttttc gacagcactt tcaagccaaa ttacataccg ggaaacagcg
      721 tataggcgct gaacagtgcg ccgttatacg acatctcaaa gtggcagtgg ttgccggtgg
      781 agttgccggt ggaaccgaca tagccgatgg tctggccctg gctgacggcc tggccctggg
      841 taacggcaat ttcggacata tggccgtaca gggtacggta gccgttgcca tggtcgattt
      901 cgacatagtt gccgtaggag taatgccagc cggcagcaac gaccgtgccg gagtcggacg
      961 caataatggt cgtgtggtag gccgcgcaga tatctgcgcc tcggtggccg ttgcccatcc
     1021 agcggctgac atacttgtag cccggcacag gccagatgaa ggaaccagag ccgagcttgg
     1081 cgaccatgcc gtttttgagc ttggtaccgg tgacggtgat ttccggcgtg ggggcctgca
     1141 gcacattata ggccacagcc tggcggtcag tgaccgcacc gtcgatcatc gtggtttcgt
     1201 aggtgatttc ttccgagccg tccacgccct cctgcacggt cacgaccttg ccgaaatcgt
     1261 actggtcgga ctcggagttc tgggtgtcaa agggaattgc cacggtctcg gtgtcgcggc
     1321 ggacgacctt gaccttgata agctctgccg aggatgcgcc ggtcgtaatg acggtgccct
     1381 cgggaatttc ctggtcaagg gagagcaggt cggggttcat ctgtgccagc gagtcaaaat
     1441 tgacgccggt ggtgtcgata acgctctgta cggtatcacc ctcgacagca gtgtaggtgt
     1501 ggataccgcc gccctcgctc agcgcgctga tcacatcggc gtaggagctg acggattcct
     1561 gcaggtaaac gccgtccacc aggcggatac tgtgggcaaa cgccgtgtag atgctgctgt
     1621 ccatggcatc ctcaaagggg gccttgatgt tttccagata ggtgcgcagg tggtcgccct
     1681 cagtggtgac atagagcagc tggtcgtcca cataaacagc cgtgccgttg atgatctcgt
     1741 cactggcggt acgcagaatg gcgttggcaa tctcgctctc ggtcat
//
LOCUS       DBMDSQ010000386         1782 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_14324_length_1782_cov_1.751338, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000386 DBMDSQ010000000
VERSION     DBMDSQ010000386.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 1782)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1782)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1782
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14324_length_1782_cov_1.751338"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..619
                     /locus_tag="ACZPAX_10325"
     CDS             <1..619
                     /locus_tag="ACZPAX_10325"
                     /EC_number="2.1.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005922295.1"
                     /note="Catalyzes the formylation of AICAR with
                     10-formyl-tetrahydrofolate to yield FAICAR and
                     tetrahydrofolate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolecarboxamide
                     formyltransferase"
                     /protein_id="HIR1114330.1"
                     /translation="GRQGLDISNETMLQNIVTENQFISDEQRRDLVISLIVLKYTQSN
                     SVCYVQDGQTIGVGAGQQSRVHCTRLAGQKADNWQLRHMPKVLALPFRDDVAKPNRDN
                     AIDVYIGDTPEDVIGDDVWAETFTEQPAPLTAEEKRTWLDAVTGVSLGSDAFFPFGDN
                     IERARRSGVTAIVQPGGSIRDQQVIDTCNKYGIAMAFCGIRLFHH"
     gene            687..>1782
                     /gene="purD"
                     /locus_tag="ACZPAX_10330"
     CDS             687..>1782
                     /gene="purD"
                     /locus_tag="ACZPAX_10330"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HIR1114331.1"
                     /translation="MKILVVGGGGREHAIIRALKKSPRCTEIWCAPGNGGISYDATCK
                     AIPATDVDAMVKFAKEEAFDYVVVAQDDPLALGIVDALAAVGIPAFGPDKAAARIEAS
                     KVFSKDLMKKYGIPTAKYETFDDPEKVMDYIKAEGKYPVVIKADGLALGKGVLICQNE
                     QEAADGVKEIMLDKKFGASGNHVVVEEFLTGPEVSVLSFCDGKTVKPMVSSMDHKRAL
                     DHDEGLNTGGMGTVAPNPYYTPEIAERCMKEIFLPTVAAMQAEGCPFKGCLYFGLMLT
                     PDGPKVIEYNCRFGDPETQVVLPLLESDLLTVMESTTNGTLDTTEVKFRDGAAACVIL
                     ASGGYPLSYEKGKEISGLAAGQLDAADVTVY"
BASE COUNT          365 a          542 c          555 g          320 t
ORIGIN      
        1 gggccgccag ggtctggata tctccaacga gacgatgctg cagaatatcg tgactgagaa
       61 ccagttcatc agtgacgagc agcgccgcga tctggtcatc agcttgatcg tgctgaaata
      121 cacccagtcc aacagtgttt gctacgtgca ggacggccag accatcggcg tcggcgcggg
      181 ccagcagagc cgcgtgcatt gcacccgcct ggcaggccag aaggccgaca actggcagct
      241 gcgccatatg ccgaaagtat tggccctgcc cttccgcgac gatgtggcaa agcccaaccg
      301 cgacaatgcc attgacgtgt acatcggcga cacgccggag gatgtcatcg gcgatgatgt
      361 ctgggccgag accttcaccg agcagcccgc cccgctgact gccgaggaaa agcgcacctg
      421 gctggacgcc gtgaccggcg tttccctggg cagcgacgcg ttcttcccct tcggcgacaa
      481 cattgagcgc gcccgccgca gcggcgtgac cgccattgtg cagccgggcg gctccatccg
      541 cgaccagcag gtcatcgaca cctgcaacaa gtacggcatt gcgatggcat tctgcggcat
      601 ccgcttgttc catcactaag caatattcaa cgcgcctgcg gcgtattttg cgtcgcgggc
      661 agttggtgtt ttacaggagg ttcctcatga aaattctggt cgttggcggc ggcgggcgtg
      721 agcacgccat catccgtgcg ctgaaaaaaa gcccccgctg cactgagatc tggtgcgcac
      781 ccggcaacgg cggcatcagc tacgatgcca cctgcaaggc catccctgcc accgatgtgg
      841 acgcgatggt caagtttgcg aaagaagaag ccttcgatta tgtagtcgtc gcccaggatg
      901 acccgctggc cctgggcatt gtcgatgcac tggctgctgt cggcatccca gccttcggcc
      961 ccgacaaggc cgcggcccgc attgaggcca gcaaggtgtt cagcaaagac ctgatgaaga
     1021 aatatggcat tcctacagcg aaatatgaga cgttcgacga tcccgaaaag gtcatggact
     1081 acatcaaggc cgagggcaag tacccggtcg ttatcaaagc ggacggcctg gcgctgggca
     1141 agggcgttct gatctgccag aatgagcagg aggccgccga cggcgtcaag gagatcatgc
     1201 tggacaaaaa gttcggcgcg tccggcaacc atgtggttgt ggaagagttc ttgaccggtc
     1261 ccgaggtcag cgtactgtcc ttctgtgacg gcaagaccgt gaagccgatg gtgtccagca
     1321 tggaccacaa gcgtgcgttg gatcacgatg agggcctgaa caccggcggc atgggcacgg
     1381 tggccccgaa cccctactac acgccggaaa tcgccgagcg ctgcatgaag gagattttcc
     1441 tgccgacggt cgccgccatg caggccgagg gctgcccctt caaggggtgc ctctactttg
     1501 gcctgatgct gacgcccgac ggccccaagg tcatcgagta caactgccgc ttcggcgacc
     1561 ccgagaccca ggtcgtgctg cccctgctgg aaagcgacct gctgaccgtg atggaatcga
     1621 cgaccaacgg cacgctggat acgaccgagg tcaagttccg cgacggcgcg gcggcctgtg
     1681 tgattctggc cagcggcggg tatccccttt cttatgagaa gggcaaggaa atttccggcc
     1741 tggctgcggg ccagcttgac gctgcggacg tgacggtgta tc
//
LOCUS       DBMDSQ010000387         1781 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_14345_length_1781_cov_0.920238, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000387 DBMDSQ010000000
VERSION     DBMDSQ010000387.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 1781)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1781)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1781
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14345_length_1781_cov_0.920238"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1623)
                     /locus_tag="ACZPAX_10335"
     CDS             complement(<1..1623)
                     /locus_tag="ACZPAX_10335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1114332.1"
                     /translation="MNKVSRIAIPPAVQGLLPAFFCIFSGVIDRLYLDIDSFLVSMTC
                     NGLYGSGAVCPVIHPLLAIVLGRLAPLAPSVDWFTLISRVLVALTAWWVGMVIAWCIG
                     SAPKRIFCLVSFSILLFRFPLFNANYTVHCAFFAMAGVTTLVLALRRTMPRGSLFWAA
                     WFLCLAILWRPEGAALMLPFLLLDLVVLALRHALPLTKVKKVLLPALIPPLLLVAFSA
                     LLPLFSAQYAAGKAYSDARRAFVDYPSRPWAEVADQVTALGLDENDYETLSSSVLIDT
                     SIADTATLQELAVIAKKGLSTEGLDTLGRDFVAAFSTLQLRVFCILTVFFLVVVCLSG
                     SPLLVKLESLLAVLGSLLITLYYLYSGRLPERLFVSIFLMQFSVLLPLFLGAASAPRA
                     VRIRFWKAACLLSACCLCLLLVKNRHNYHVTQLAIHAGTAAADDTALLPADDPDTVYL
                     WDSMSMALYMTEHYMMEGKLPSTAFVRQNLAWGEWNTSGQPFYQVLLADLDLENPMQC
                     LLIRPHTYLVVKSEFAPHLQTWLQEHYTLTATL"
BASE COUNT          468 a          488 c          549 g          276 t
ORIGIN      
        1 tagggtggca gtgagggtgt agtgctcttg cagccaggtt tgcaggtgcg gagcaaactc
       61 ggatttgacg accaggtaag tgtgggggcg gatcagcagg cattgcatgg gattttcaag
      121 gtcaagatcg gccagcagga cttgatagaa cggctggccg ctggtgttcc actcgcccca
      181 ggcaaggttt tgccgcacaa acgctgtgct ggggagctta ccctccatca tataatgctc
      241 ggtcatgtac agggccatcg acatcgagtc ccagaggtag acggtatccg ggtcgtcggc
      301 aggcagcagc gcggtgtcgt cggcagcggc ggtaccggca tggatggcca gctgtgtgac
      361 gtggtaatta tggcggttct tgaccagcag caggcacagg cagcaggcgg aaagcaaaca
      421 ggcggctttc cagaaccgaa tgcgtacagc gcgcggcgca gaggcagcgc ccagaaacag
      481 cggcagcagc acgctgaatt gcatcaaaaa gatcgagaca aacagccgtt ccggcaggcg
      541 gccgctgtac aggtagtaca gggtgatcag caggctgccc aatacggcca gcagggattc
      601 gagtttgaca agcagcggcg aaccggaaag acagaccacc accaggaaaa acaccgtcag
      661 aatgcagaat acccgcagtt gaagtgtgga gaacgcagcc acaaagtcac ggcccagcgt
      721 gtcgagcccc tcggtcgaaa ggcctttttt ggcgatgaca gccagttctt gtaaagtggc
      781 tgtgtcagca attgaagtat ctatcaggac gctggacgaa agtgtctcgt agtcgttttc
      841 gtccagcccc agcgctgtaa cttggtcggc gacctcggcc caggggcggc ttgggtaatc
      901 cacaaaggcc cgtcgggcat cgctgtacgc cttgcctgcg gcatactgtg ccgaaaacag
      961 cggcagcagc gccgagaagg caaccaacag cagcggcggg atcagggcgg gcagcaacac
     1021 ttttttgacc ttggtaaggg gcagcgcatg gcgcagggcc agcaccacaa gatccagcag
     1081 aagaaacggc agcatcaaag ctgccccctc gggccgccac aggatggcca gacagaggaa
     1141 ccatgcggcc cagaacaaag acccgcgcgg catagtcctg cgcagggcca gcaccagtgt
     1201 ggtgacacca gccatagcaa aaaaggcgca atgcaccgtg taattggcgt tgaatagcgg
     1261 aaaccggaac agcagaatgc tgaacgaaac caggcagaag atccgtttgg gggcggatcc
     1321 gatgcaccag gcaatcacca tgccgaccca ccaggccgtt agtgctacca gaacgcggct
     1381 gatcagggtg aaccagtcga ccgagggggc cagcggtgcc agccgcccca gcacgatagc
     1441 cagcagcgga tgaatgaccg ggcagacggc gccgctgccg tacagcccgt tgcaggtcat
     1501 gctgaccagg aaagaatcaa tgtccagata cagccggtcg atcacgcccg aaaagatgca
     1561 gaaaaaggcg ggcagcagcc cctgcacggc aggcgggatc gcaatacggg aaaccttgtt
     1621 caaagcatgc agctcctttt ggcaaatcaa tgtaccaaca ttttacatcc tgcggggaaa
     1681 aaacacaacc cgaatcgccc aaacacagcc cgaaaaagag cataaagaaa ggggcaaaga
     1741 acggcgtcaa atccagttca ttatacttag gaagcatata c
//
LOCUS       DBMDSQ010000388         1772 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_14434_length_1772_cov_1.435069, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000388 DBMDSQ010000000
VERSION     DBMDSQ010000388.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 1772)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1772)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1772
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14434_length_1772_cov_1.435069"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            28..>656
                     /locus_tag="ACZPAX_10340"
     CDS             28..>656
                     /locus_tag="ACZPAX_10340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005932827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine biosynthesis TtcA family
                     protein"
                     /protein_id="HIR1114333.1"
                     /translation="MERQLETYQKIERSIIKKFRKQLWTPFIVAVKRYELIQAGDKIA
                     VCISGGKDSMLMAKLLQELQRHSEVPFELVYLVMDPGYNEINRQKIESNAALLHIPIT
                     VFETNIFDVANNSDKSPCYLCARMRRGHLYKKAQELGCNKIALGHHFNDVIETTVMSM
                     FYGSQLQAMPPKLHSTNFPGMTLIRPMYCIREEDIIAGGGVSITQEQIQ"
     assembly_gap    657..666
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <667..986
                     /locus_tag="ACZPAX_10345"
     CDS             <667..986
                     /locus_tag="ACZPAX_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013497599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA 2-thiocytidine biosynthesis protein TtcA"
                     /protein_id="HIR1114334.1"
                     /translation="AWRRYNELEFIQCACRFTEACTICDNGGGGSKRQEVKILLRRLM
                     RDNPNIENSIFRSIHAVSLDTMPGYKTEGEEHSFLERFDRIEEALKAKKASPSGEAVR
                     EAD"
     gene            complement(1100..>1772)
                     /locus_tag="ACZPAX_10350"
     CDS             complement(1100..>1772)
                     /locus_tag="ACZPAX_10350"
                     /inference="COORDINATES: protein motif:HMM:NF024242.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1114335.1"
                     /translation="TLEYEGQTRALRAGDCAFLDCRRLYRHYTGDDLWTLRWVHFYGP
                     NMGAIYKKYQERGGQPSFHAEDAAAYTTSLETLYTLAGSDNYVRDMQICEKLMALLTL
                     LMQESWHPDTARTTAGKRQNLQEIKDYLDTHFAEKITLDALAERFYINKFYLTRVFKE
                     QFGQTVTNYLVQLRITQAKRLLRFSDKNVEAIAQECGLNDANYFSRLFKKVEGTTPGE
                     YRRQW"
BASE COUNT          398 a          490 c          481 g          393 t
ORIGIN      
        1 tccctgacaa ataaggagaa ttgaccgttg gaacgccaat tagagactta tcaaaaaatt
       61 gaacgcagca ttatcaaaaa attccgcaag cagctgtgga cgcccttcat tgtggcagtc
      121 aagcggtacg agttaattca agcgggggat aagatcgccg tctgcatttc cggcggtaag
      181 gactccatgc tgatggcaaa gcttttgcag gagttgcagc gccacagcga ggtgccgttt
      241 gagctggtct acctcgtcat ggatcccggc tataacgaaa tcaaccgcca gaaaatcgag
      301 agcaacgccg cgctgctgca cattcccatc acggtgtttg agacgaacat ctttgatgtc
      361 gccaacaaca gtgacaagtc gccctgctac ctctgcgccc ggatgcgccg cggccaccta
      421 tacaagaagg cgcaggaact tggctgcaat aaaatcgcgc tggggcatca cttcaatgac
      481 gtcatcgaga cgacggtcat gagtatgttc tacggctccc agctgcaggc tatgccgccg
      541 aagctgcaca gcacaaactt tccgggcatg accctcatcc gcccgatgta ctgcatccgc
      601 gaggaggata tcattgcagg cggcggtgtt tccattacac aggaacagat ccaggannnn
      661 nnnnnnttgc ctggcggcgg tataacgagc tggaatttat ccaatgcgcc tgccgcttta
      721 ccgaggcctg caccatttgt gacaatggcg gtggcggatc caagcggcaa gaggtcaaga
      781 tcctgctgcg ccgtttgatg cgcgacaatc cgaacatcga gaacagcatt ttccgcagca
      841 tccatgccgt ctcgctggac accatgcctg gttacaagac cgagggcgag gagcattcgt
      901 tcttggagcg gtttgaccgg attgaggaag cgttaaaagc aaaaaaggct tctccttcgg
      961 gagaagctgt ccgcgaagcg gactgacgag gggcggcttt atcgctataa ctcatttgag
     1021 gggggcggct atggccgcct cttccctcat ccggcccttc gggccacctt tccccaaggg
     1081 ggaaggcttt tttattatcc taccactgcc gccgatattc ccccggtgtt gtcccctcca
     1141 ccttcttgaa taaccgggaa aaataattgg catcattcag cccgcactct tgggcgatgg
     1201 cctctacatt tttatcactg aaccgcagca gccgcttcgc ctgggtgatg cgcagctgca
     1261 ctaaatagtt cgtgacggtc tggccgaact gctccttgaa cacgcgggtc aaataaaatt
     1321 tgttgatgta aaaccgctcg gccagggcgt ccagcgtgat tttctcggca aaatgggtat
     1381 caagataatc cttgatttcc tgcaaattct gccgtttgcc agcggtcgtg cgggctgtgt
     1441 cagggtgcca gctctcctgc atcagcagcg tcagcaaggc catgagtttt tcgcagatct
     1501 gcatatcgcg gacatagttg tcagaccccg ccagcgtata cagtgtttcc aaagacgttg
     1561 tgtaggccgc cgcatcttcg gcgtggaaac tcggctgacc accgcgctcc tggtattttt
     1621 tatagatggc ccccatgttc ggaccgtaaa agtgtaccca gcgcagcgtc cataagtcgt
     1681 cgccggtgta gtggcggtag aggcggcggc agtcgaggaa tgcgcagtcc ccggcgcgca
     1741 gggcgcgagt ctggccctca tattccaacg ta
//
LOCUS       DBMDSQ010000389         1755 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_14649_length_1755_cov_1.730955, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000389 DBMDSQ010000000
VERSION     DBMDSQ010000389.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 1755)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1755)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1755
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14649_length_1755_cov_1.730955"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="ACZPAX_10355"
     CDS             complement(<1..209)
                     /locus_tag="ACZPAX_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005945729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HIR1114336.1"
                     /translation="MPDLGLNVLLKGKNAARLLGGLWVALRISLISVVISIPLGILLG
                     VLMSGKNPIVKAILRVYLEIIRIIP"
     gene            complement(190..849)
                     /locus_tag="ACZPAX_10360"
     CDS             complement(190..849)
                     /locus_tag="ACZPAX_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005945726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HIR1114337.1"
                     /translation="MDWNVIAQYLPLYKKAAWLTLRLGLAGIVIAIVVGLVCALVQYY
                     KVPVLRRIVGAYIELSRNTPLLVQLFFIYYGLPKIGIKTNAEICGIVGLAFLGGSYMA
                     EAFRSGLESIEPIQTESAMSLGMDHAQTMRYIILPQAMSISVPAFVANVIFLLKETSV
                     FSAISLMDLMFTAKDLIGLYYKTTECLFLLVVFYLLILLPVSLLGSWLERRLRYAGFG
                     A"
     gene            complement(1322..>1755)
                     /locus_tag="ACZPAX_10365"
     CDS             complement(1322..>1755)
                     /locus_tag="ACZPAX_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EF-Tu C-terminal domain-related protein"
                     /protein_id="HIR1114338.1"
                     /translation="MTTTITGLEMFRKSLEFAQAGDNIGALLRGVDRDQIERGQCLCQ
                     PGSVHPHTTFDGHVYVLKKEEGGRHTPFFNNYRPQFYFRTTDVTGIITLPEGTEMCMP
                     GDNVDMHVELITPVAMEEGLRFAIREGGHTVGSGVVSKIEK"
BASE COUNT          401 a          491 c          501 g          362 t
ORIGIN      
        1 tgcggtatga tgcggatgat ttccagatac acgcggagaa tagctttgac gatgggattc
       61 ttgccgctca tcagcacacc cagcaggatg cccagcggga tgctgatgac gacggaaatc
      121 aggctgatgc gcagggccac ccacaggccg cccagcaggc gggccgcgtt tttgcccttg
      181 agcagtacgt taagccccaa atccggcata gcgcagcctc ctttccagcc aactgcccag
      241 cagcgacact ggcagcagaa tcagcaggta aaagaccacc agcaggaaca ggcactcggt
      301 ggttttgtag tacaggccga tgaggtcttt cgcggtgaac atcaggtcca tcaggctgat
      361 ggcgctgaac acgctggtct ccttcagcag gaaaatgacg ttggcgacga acgccggcac
      421 actgatggac atagcctgcg gcaggatgat gtaacgcatc gtctgcgcgt ggtccatgcc
      481 caggctcatg gcgctctcgg tctgaatcgg ctcgatggat tccaacccgc tgcggaacgc
      541 ctccgccatg tagctgccgc ccaggaacgc cagacccaca ataccgcaga tttcggcgtt
      601 ggtcttgatg ccgattttcg gcaggccata atagatgaaa aacagctgca ccagcaacgg
      661 cgtattgcgg ctgagctcga tgtaggcccc cacgatgcgg cgcagcacag gcaccttgta
      721 atactggacc agcgcacaga ccagcccgac gactattgcg atcacgatgc ctgccaggcc
      781 aaggcgcagg gtcagccacg cggccttttt gtacagtggc aggtattgtg cgatcacatt
      841 ccaatccacg aaagtctccc cttccctatc gggtcatgaa caaatttggg tacaaaaaaa
      901 tccgggcttt cgtaacgaaa gttcccggac gaaggtcaag ttcaacacat aggggtgctg
      961 gtgcgcccca tagaccatgc ccggtgacag tacatacgac aacaacacat agcagacatg
     1021 gcagcgttcc cctttctggt ttttggtttg cctttgttca aacccgatgc ctgtataata
     1081 tacctattta cccagaatgt caataggttt ataggattta tttgactttt tccgcaaaaa
     1141 aatccctctg cgtttgtgga tggtgcacaa acgcagaggg atgtgttcta ttgcagggag
     1201 ggagcttgtc ccctctgcgg ggcgtcgagg acgccgcccc ctacaaacat cggtttccaa
     1261 tacaaaaaag ggcgctgccc ttacggacag cgcccttagt attgcttttc agagcaagaa
     1321 attacttctc gatcttggaa acaacgccgg aaccaacggt gtggccgccc tcgcggatag
     1381 cgaaacgcag gccctcttcc atggcgacgg gggtgatcag ctcaacgtgc atatcgacgt
     1441 tgtcgccggg catgcacatc tcagtgccct cgggcagggt gatgatgccg gtaacgtcgg
     1501 tggtgcggaa gtagaactga ggacgatagt tgttgaagaa cggggtgtga cggccgccct
     1561 cttccttctt caggacgtaa acgtggccgt cgaaggtggt gtgggggtga acagaaccgg
     1621 gctggcacag gcactggcca cgctcgatct ggtcgcggtc aacaccacgc agcagggcgc
     1681 cgatgttgtc gccagcctga gcgaactcca ggctcttgcg gaacatctcc aggccggtga
     1741 tggtggtggt catct
//
LOCUS       DBMDSQ010000390         1754 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_14666_length_1754_cov_1.568663, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000390 DBMDSQ010000000
VERSION     DBMDSQ010000390.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 1754)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1754)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1754
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14666_length_1754_cov_1.568663"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1673
                     /gene="recJ"
                     /locus_tag="ACZPAX_10370"
     CDS             <1..1673
                     /gene="recJ"
                     /locus_tag="ACZPAX_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HIR1114339.1"
                     /translation="RPALESLAEKGFKLVVTVDNGISAIEEAACAKELGLDLIVTDHH
                     LPGDTLPDAIAVVDPKRPDDESPFKDLCGAGVAFKLCAALEGCDPAELLEMYGELAAL
                     GTVADVMPLVGENRTIVREGLSLLQDTMRPGLHALLESAGCSGKPVTADTVSYSLAPR
                     LNAAGRMDNAAVALKLLLCGDEDQATGIAERLAEINTERQQTEQQVFAAALQTLEADP
                     ARTRDRVLVVSGEDWHPGVIGIVAARLMERFSRPAIVISTHEGEGRGSGRAPDPFDLH
                     SALAGCAHYLIRFGGHAAAAGVEIEEENLSAFRQAINAWAADHAAQPGPVSLGMDAAV
                     TLAELSLSNVEELARLAPFGRENPTPVLLVQHAFVDGLWPMGAEGRHTRVRLRQGNDT
                     LFASIFGVAPQSFAYPVGSEVEAALEVSVFNGRSGPMVSAHLKAIRPADLGNTPSEQA
                     AWFEAFRTGGDLPADRAAALLPQRADTVALYRRIRTGQVVSADLQPVFAAQGPQNTGK
                     ALTSLTALQELGLIEEQNGHWQPVAVTAKQDLASAPVLQRLAAMAKQG"
BASE COUNT          291 a          579 c          600 g          284 t
ORIGIN      
        1 cccgcccggc gctggaaagc ctggccgaaa agggctttaa gctggtggtg accgtggata
       61 acggcatttc ggccatcgag gaagccgcct gcgccaagga actgggcctt gacctcatcg
      121 tcacggacca ccacctgccc ggtgacacgc tccctgacgc gattgccgtc gtagacccca
      181 aacggcccga cgacgaaagc ccgtttaaag atttgtgcgg cgcgggcgtg gcgttcaagc
      241 tctgcgccgc gctggaaggc tgtgaccccg ccgagctgct ggaaatgtac ggcgagctgg
      301 ctgccctggg caccgtggcc gacgtaatgc cgctcgtggg ggagaaccga accatcgtcc
      361 gcgaggggct gagccttttg caggacacga tgcgccccgg cctgcacgcc ctgctggaaa
      421 gcgcgggctg cagcggcaaa cctgtcacgg cggacacggt cagttacagc ctggccccgc
      481 ggctgaacgc ggcgggccgc atggataacg ccgccgtggc gctgaaattg ctgctttgcg
      541 gcgacgagga tcaggccacc ggcattgccg agcgcctggc cgagatcaac accgaacgcc
      601 agcaaaccga acagcaggtg ttcgcagcgg cgctgcaaac gctggaggct gaccccgccc
      661 gaacccgcga ccgggtgctc gttgtcagcg gtgaggactg gcaccccggt gtcattggca
      721 tcgtggcggc acggctcatg gagcggttca gccgcccggc catcgtcatt tcaacgcacg
      781 agggtgaggg acgcggcagc ggccgtgcac cggacccctt tgatctgcac agcgctctgg
      841 cgggctgtgc ccactatctg atccggtttg gcggccacgc cgccgccgcg ggtgtggaga
      901 ttgaggaaga aaatttatcc gccttccgtc aggccatcaa cgcctgggcc gccgaccacg
      961 ccgcccagcc cgggccggtc agcctgggga tggatgccgc tgttacgctg gcggagctga
     1021 gcctgtccaa tgtggaggaa ctggcccgtc tggccccctt tggccgggaa aacccgaccc
     1081 cggtgttgct ggtgcagcac gcctttgtgg atggtctgtg gcccatgggc gccgagggac
     1141 gccacacccg cgtgcggctg cgccagggta acgacacgct gttcgcctcg attttcggcg
     1201 tagcaccgca gtcgttcgcg tatcctgtcg gcagcgaggt cgaagctgcg ctggaagttt
     1261 ccgtctttaa cggccgcagc gggccgatgg tctccgcaca cctgaaagcc atccgcccgg
     1321 ccgatttggg caacacgccc agcgaacagg ccgcctggtt cgaggcgttc cgcaccggcg
     1381 gcgatttgcc cgccgaccgt gcagccgctc tgctgccgca gcgggccgac accgtcgcgc
     1441 tctatcggcg catccgcacc gggcaggtcg tttcggcgga tctgcagccg gtctttgcgg
     1501 cgcagggccc gcagaacacc ggcaaggcgc tgacaagcct gacggccctg caggagcttg
     1561 gccttatcga agagcaaaac ggccactggc agccggtggc cgtgaccgct aagcaggatc
     1621 tggcgtccgc gccggttttg cagcgtttgg cggccatggc gaaacaggga taaaatgcgg
     1681 ttttatccgg gattgcaggg gcggatgctc gcatccgccc gcggggcgtg gcggcaacgg
     1741 cgacgttccc gggc
//
LOCUS       DBMDSQ010000391         1743 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_14794_length_1743_cov_1.586480, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000391 DBMDSQ010000000
VERSION     DBMDSQ010000391.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 1743)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1743)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1743
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14794_length_1743_cov_1.586480"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..917)
                     /locus_tag="ACZPAX_10375"
     CDS             complement(<1..917)
                     /locus_tag="ACZPAX_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005926349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR1114340.1"
                     /translation="MKRTLLLTLPALLLAGCAAASEPTQTDALAIESRYPLDYAQQFT
                     VDECAGGYSLITIDGSRYLVVPEGAAVPAELDGDIVVLQQPIENIYLVSSSAMDPIIS
                     IGGLGAVALSGTQAENWYLDAARTAMEQGQIVYAGKYSAPDYETILVADCGLAIENTM
                     IYHTPEVKEQLEKFGVPVLVERSSYESGPLARMEWVKLYGILLGKTNEAERVFADAEQ
                     RIAPLADEAPTGKTVAFFSITSNNLATVRKGGDYVAEMIRMAGGSYAFADLTDSGNNL
                     ATVNISLEDFYAGAKDADVLIYNSTIEGEL"
     gene            complement(914..1690)
                     /locus_tag="ACZPAX_10380"
     CDS             complement(914..1690)
                     /locus_tag="ACZPAX_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005935013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron transporter"
                     /protein_id="HIR1114341.1"
                     /translation="METAPEATEAPAATEAPAAALPDGVYTAEFKTDSSMFHASEAVD
                     GMGTLTVENGVMTFHVSLQSQKIVNLYLGMAADAPDHESDWLQPTVDTVTYSDGTSDE
                     VYGFDIPVQAVDTDFQLAILGTKGTWYDHTVSVADAQPAADETAASAEAPADGSYTCA
                     VTLEGGSGRATVESPAALTVADGTMTATIVWSSPNYDYMLIDGEKYLPTNTEGNSTFE
                     IPVAALDTALAVTADTVAMSTPHEIDYTLTFDSATLTAAE"
BASE COUNT          299 a          502 c          580 g          362 t
ORIGIN      
        1 agttcaccct caatcgtgct gttataaatc agcacgtcgg catccttggc cccggcgtag
       61 aaatcttcca gcgaaatatt cacggtcgcc aggttgttgc cgctgtcggt caaatcggca
      121 aaggcgtaac tgccgcccgc catcctgatc atttcggcaa cgtaatcgcc gcctttgcgc
      181 acagtggcaa ggttgttgga ggtgatggag aaaaacgcga cggtcttgcc ggtgggggct
      241 tcgtccgcca aaggggcgat gcgctgctcg gcatccgcaa aaacacgctc tgcttcgttg
      301 gttttgccca gcagaatgcc gtagagcttg acccattcca tgcgggccag cggaccgctc
      361 tcgtagcttg agcgttcgac cagcaccggc acaccgaatt tttccagctg ctccttcacc
      421 tcgggggtgt ggtagatcat cgtgttttcg atggcaaggc cgcagtcggc gaccaaaatc
      481 gtctcgtaat ccggggcgct gtacttgccc gcgtagacaa tttgcccctg ttccatggcg
      541 gtgcgggcag cgtcgagata ccagttttcg gcctgcgtgc cagagagtgc caccgccccc
      601 agcccaccaa tgctgatgat gggatccatg gcggaggaag aaacgaggta gatattctcg
      661 ataggctgct gcaaaacgac aatgtcgccg tccagttcgg cgggcacagc ggccccttcg
      721 ggtacgacca gatagcggct gccgtcgatg gtgataaggc tgtacccgcc cgcgcattcg
      781 tccaccgtga actgctgcgc ataatcgaga gggtagcggc tttctatcgc aagtgcgtcg
      841 gtttgtgtcg gttccgatgc cgccgcgcag cctgccagca gcagggcggg cagggtcaaa
      901 agtaaagtgc gcttcattcg gctgcagtca gcgttgcgct gtcaaaggtc agcgtgtagt
      961 cgatctcgtg cggggtgctc atggcgacag tgtctgccgt gacggccagc gccgtgtcca
     1021 gcgcggcgac gggaatctca aacgtggaat tgccttcggt gttcgtgggc aggtactttt
     1081 caccgtcgat gagcatatag tcgtagttcg ggctgctcca cacgatggtg gcggtcatcg
     1141 tgccgtcggc cacggtcagg gcggcagggc tttcgacggt agcgcggccg ctgccgcctt
     1201 ccagcgtcac ggcacaggta tagctgccgt cggcgggtgc ctcagcggag gctgcggtct
     1261 cgtcagcggc gggctgggca tcggccacac tgacggtatg gtcgtaccag gtgcccttgg
     1321 tgcccaggat ggccagctgg aagtcggtat ccacggcctg caccgggata tcaaagccgt
     1381 agacttcgtc gctggtgcca tcagagtacg tcacggtgtc aacggtgggc tgcagccagt
     1441 cgctctcgtg gtcgggggcg tcggcggcca tgccaaggta caggttgacg attttctggg
     1501 attgcaggct gacgtggaac gtcataacgc cgttttccac ggtcagggtg cccatgccgt
     1561 ccacggcctc gctggcatgg aacatactgc tgtcagtctt gaactcggcg gtgtaaacgc
     1621 cgtcgggcag ggcggcggca ggagcttcgg ttgcggcggg ggcctcggtg gcttcggggg
     1681 cggtctccac ggtggaggca gccgcggatg cactctcggc ggtggtggat gcggatgcgc
     1741 cgc
//
LOCUS       DBMDSQ010000392         1743 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_14798_length_1743_cov_0.982339, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000392 DBMDSQ010000000
VERSION     DBMDSQ010000392.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 1743)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1743)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1743
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14798_length_1743_cov_0.982339"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            135..947
                     /locus_tag="ACZPAX_10385"
     CDS             135..947
                     /locus_tag="ACZPAX_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006876916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HIR1114342.1"
                     /translation="MSTTYSDNIILVAEAVAGDAATVNTWITDTQANDPKLVFVGEGL
                     LSITSILDPAKLQFTQDDFVFVENLYSSIFVLSADAKLNINSMDDLKAYVESGEQVSV
                     AVNGATSSEAFLAAALFGSMGAGEHLKLVPYTSAAEAAQAVAKGETNFAVSHQSQILE
                     TYEQGGVSIVCAFDEKAIENGPFSGVEGVGQYGYPYFRNRCFIMARAGTDAATVANLK
                     DLYDQILADDEVKTWLQDTMLLEVDTMSTEDVNAHVENVKGIVNEYKDIVAG"
     gene            1035..1550
                     /locus_tag="ACZPAX_10390"
     CDS             1035..1550
                     /locus_tag="ACZPAX_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006876917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HIR1114343.1"
                     /translation="MKNWVDRLDARLDAIGANLAKKEVHIPTDLAGGILFFLFAIVIL
                     LVTPQQVTISEKDIINGRMFPRLLMIVMMVCCALLIGKELYKIARKLPITYKTVNLLV
                     EIKALVILAILVLTYVVCRVTNLFAAGSVFCCIGFLLYFRCKKPLYYAVTVILAVAIW
                     AAFRFGLNVNF"
     gene            1563..>1743
                     /locus_tag="ACZPAX_10395"
     CDS             1563..>1743
                     /locus_tag="ACZPAX_10395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006876918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HIR1114344.1"
                     /translation="MLQYIIPASGLLFTLQNIIWINLGVFIGAVFAAIPGLSVILCVI
                     LFLPLTYQLTAIPGMM"
BASE COUNT          377 a          489 c          461 g          416 t
ORIGIN      
        1 ctgccccagc tgcaagcgat gggaacacgg ctggcggcgc aaacacgccc ggcggcaaaa
       61 ctgccacctg gaccgtcacc tgtccatggg cgccctccgg agtggccgcc atggtgagcc
      121 aaaaggctgc ggccttgtca acgacatatt cagacaatat cattctggta gccgaagccg
      181 ttgcgggcga cgcggctacc gtcaacacat ggatcactga cacacaggcc aatgatccga
      241 agcttgtctt tgtgggcgag ggcctgcttt caatcacctc gattctagac cccgccaagc
      301 tccagtttac acaggacgat ttcgtatttg ttgaaaattt gtactcctcc atcttcgttt
      361 tgtccgccga cgccaagctg aacatcaaca gcatggatga tctgaaggct tacgtcgaaa
      421 gcggcgagca ggtcagcgtt gccgttaacg gtgcgaccag ctcggaagcg ttcctggccg
      481 cggctttgtt cggttccatg ggcgccggcg agcatttaaa gctggttccg tacacctccg
      541 ccgccgaagc ggcccaggct gtcgccaagg gggagacaaa ttttgccgtg tcccatcagt
      601 cgcagatcct cgaaacctac gagcagggcg gtgtgagcat cgtctgcgcc ttcgatgaaa
      661 aggccattga aaacggcccc ttctccggcg ttgagggtgt gggacagtac ggttatccat
      721 acttccgcaa ccgctgcttc attatggcgc gcgccggtac cgacgccgcg acagttgcca
      781 atctgaaaga tctttacgat cagatactgg ccgacgacga ggtcaagacc tggctccagg
      841 acaccatgct gctcgaggtc gatacgatga gcaccgagga cgtcaacgcc catgtggaaa
      901 acgtcaaagg cattgtcaac gaatataagg atattgtcgc cggttgatcc cgctcaaaca
      961 cagaggatgg gatgggtttg ctcccacccc atcctttttt tacccaatct gttatcagaa
     1021 ggaggctgta ccgcatgaaa aactgggtgg atcgcctgga cgcccgcctg gatgccattg
     1081 gcgccaacct ggcaaaaaaa gaggttcaca tccccacgga cctggccgga ggaattttat
     1141 tcttcctctt tgcgattgtt atcctgttgg tcacgccgca gcaggtcaca atctcggaaa
     1201 aggatatcat caacggacgg atgttcccca ggctgctgat gatcgtcatg atggtctgct
     1261 gtgcgctgct gatcggaaaa gagctttata aaatagccag aaagctgccc attacataca
     1321 aaaccgtcaa cctgctggtt gaaatcaaag ccctggtcat tctggcaatc ctcgtgctga
     1381 cctatgtcgt ctgccgtgtt acgaacctgt tcgcggcggg ttctgttttc tgctgcattg
     1441 gctttttgct ctattttcgc tgcaaaaaac cgctttacta tgcagtcacc gttattttgg
     1501 cagtcgcaat ctgggccgcg ttccgcttcg gcctgaacgt aaatttctaa ggggggcgta
     1561 ttatgctgca atatatcatc cctgcgtcgg ggctgctgtt cacgctccag aatatcattt
     1621 ggatcaacct cggtgtgttc atcggcgcgg tattcgccgc catcccggga ttaagcgtta
     1681 ttttgtgcgt cattttgttt ttgccgctga cctatcagct caccgccatc ccgggaatga
     1741 tgt
//
LOCUS       DBMDSQ010000393         1740 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_14837_length_1740_cov_1.206223, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000393 DBMDSQ010000000
VERSION     DBMDSQ010000393.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 1740)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1740)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1740
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14837_length_1740_cov_1.206223"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..125)
                     /locus_tag="ACZPAX_10400"
     CDS             complement(<1..125)
                     /locus_tag="ACZPAX_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HIR1114345.1"
                     /translation="MRPADSWRDYELLDATNHNRLERWGQTLLIRPDPQVIWKND"
     gene            complement(162..1529)
                     /gene="glmM"
                     /locus_tag="ACZPAX_10405"
     CDS             complement(162..1529)
                     /gene="glmM"
                     /locus_tag="ACZPAX_10405"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HIR1114346.1"
                     /translation="MGKLFGTDGVRGVAGKDLTCELALQIGRGTAAVLTSLDGHRPKI
                     LIGKDTRVSGDMLESTLAAGLCSVGADVELLGVIPTPGVAYLVRKYNADAGIMISASH
                     NPMEFNGIKIFKGDGYKLPDEVENQIEAHVFNNCTDIRLAEGADIGRIKVCRTGVKDY
                     VDFLQEHIDADLTGLKVLFDCANGASARVAQELFPRLGCECGFIGTQQDGVSVNDGCG
                     STHLETLEAAVKQGGYDCGIAFDGDADRCLACDENGAEIDGDKIIALVGTDMKERGRL
                     DGNTVVVTVMSNLGFMKYMQSLGIETARTAVGDRYVLEEMRARGYAIGGEQSGHVIFL
                     HHSTTGDGELTAGKLLKVLARKKAENGAKMSDLNAVYTKFPQVLINLTADKAQKEAYK
                     EDECIAGFIEGQQKNLMGMGRVLVRVSGTEPKIRVMVEGEDQEAIKSSADRIADMIKQ
                     RILNK"
     gene            complement(1624..>1740)
                     /locus_tag="ACZPAX_10410"
     CDS             complement(1624..>1740)
                     /locus_tag="ACZPAX_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015537225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HIR1114347.1"
                     /translation="GMLTRTPRGRCVTRLAYEHLHMAVPRRFDEDDGQQSMF"
BASE COUNT          295 a          519 c          540 g          386 t
ORIGIN      
        1 tcgtcgtttt tccagatgac ctgcgggtcg gggcggatca gcaaagtctg cccccaccgc
       61 tccaggcggt tgtggttggt ggcgtccaac agttcataat cccgccagct gtcggcaggg
      121 cgcataggca attctccttt tattctatac ggtacaacgt cttatttatt caaaatacgc
      181 tgtttgatca tgtcggcgat gcggtcggcg ctggacttga tggcctcctg gtcctcgccc
      241 tcgaccataa cgcggatctt cggctcggtg ccggaaacgc ggacgaggac gcggcccatg
      301 cccatcaggt tcttctgctg gccctcgatg aagccggcga tacactcatc ctctttgtag
      361 gcttctttct gggccttgtc ggcagtcagg ttgatgagca cctgggggaa cttggtgtac
      421 acggcgttca ggtcgctcat cttggcgccg ttctcggcct tcttgcgggc cagcaccttc
      481 agcagcttgc cggcggtcag ttcgccgtcg ccggtggtgg agtgatgcag gaagatcacg
      541 tgaccgctct gctcgccgcc gatggcatag ccgcgggcgc gcatctcttc cagcacgtag
      601 cggtcgccca cggcagtgcg ggcggtctca atgcctaaag actgcatgta tttcatgaag
      661 cccaggttgg acatgacggt gacgacgaca gtgtttccat ccaggcggcc gcgctccttc
      721 atatcggtgc cgaccagtgc gatgatcttg tcgccgtcga tctcggcacc gttttcgtcg
      781 caggccaggc agcggtccgc gtcgccgtca aaggcgatgc cgcaatcgta gccgccctgc
      841 ttgacggccg cttccagcgt ttccagatgg gtggagccgc agccgtcgtt gacggaaacg
      901 ccgtcctgct gggtgccgat gaagccgcac tcgcagccaa ggcgcgggaa caattcctgc
      961 gcgaccctgg cggaggcgcc gttggcgcag tcgaacagca ctttcagacc ggtgaggtcg
     1021 gcgtcgatat gctcctgcag gaagtcgacg taatccttta cgccagtgcg gcacaccttg
     1081 atgcggccga tgtccgcgcc ctcggccaga cgaatgtccg tgcagttgtt gaagacgtgg
     1141 gcttcgatct ggttctcgac ctcatcgggc agtttgtagc cgtcgccctt gaagatcttg
     1201 atgccgttga actccatcgg gttgtgggag gcggagatca tgatgccggc gtcggcattg
     1261 tatttgcgga ccaggtaggc cacgccgggg gtagggataa cgcccagcag ctctacgtca
     1321 gcgccgacac tgcacaggcc ggcggccagg gtggattcca gcatatcgcc ggacacacgg
     1381 gtatctttgc cgatcaggat tttggggcgg tggccgtcca ggctggtaag cacggcagcg
     1441 gtgccgcggc cgatctgaag cgccagttcg caggtgaggt ccttgcccgc cacaccgcgg
     1501 acaccatcag taccaaacaa tttacccatt ctcgtcagat cctttcctat tggttgattc
     1561 ggttgattta tcattctatg ccgcttgtcg gcatggggtt agtgattttt cgcccgaagc
     1621 acgtcagaac atgctctgct gtccgtcatc ctcatcaaaa cggcgcggta cagccatgtg
     1681 cagatgctca taggccaggc gggtcacgca gcggccgcgg ggggtgcggg tcagcatgcc
//
LOCUS       DBMDSQ010000394         1737 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_14864_length_1737_cov_1.567848, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000394 DBMDSQ010000000
VERSION     DBMDSQ010000394.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 1737)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1737)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1737
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14864_length_1737_cov_1.567848"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="ACZPAX_10415"
     CDS             complement(<1..213)
                     /locus_tag="ACZPAX_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR1114348.1"
                     /translation="MKKLTALALAGCMAVSLAACGGSASTTPASSEAASTESAATSAA
                     ESAAESVTEATQGGKVGVCIYKFDDAF"
     gene            complement(642..1610)
                     /locus_tag="ACZPAX_10420"
     CDS             complement(642..1610)
                     /locus_tag="ACZPAX_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HIR1114349.1"
                     /translation="MTNCVKRLLPLALAAALAGCTMQATDSNATRYQVALVAKSKDSE
                     FWNAVFTGAEAAATEYNMQLTIAAPNSEEDYEEQDRMIEKAVEDGAQAIVFSAIDYEK
                     NAAAIDAAAAAGVTIIGIDSAVDSAHVAAYIGADNYGAGQMAAQSALDGMDGQLYVGL
                     INYEVNGANGRDREQGARDAFADSGRAEVVAAMPTHSNAVSARSEALSMLREHPEINV
                     LVAFNEATAVGAARAVQQMQRADDLWLVAFDSNIETIDALQTGVVDALIVQNTYGMGY
                     FGVESAYKLLAGQGSSVEESTVTTTRIITRDNVFAMDKQKALFPFE"
BASE COUNT          337 a          489 c          545 g          366 t
ORIGIN      
        1 gaaggcatcg tcgaacttat agatgcagac gccgacctta ccgccctgag tggcctcggt
       61 aacgctctca gctgcgctct cggcagcaga ggtagcggcg gactcagtag aggcagcctc
      121 gctggaagcg ggggtggtgg aagcggagcc gccgcaagcg gcgagggata cggccatgca
      181 gcccgcgagg gccagagcag tgagtttctt catgataagt ttctccttct ttttaagcag
      241 tggatttttt ccgggtcgct tgtagagggg cgaaccctgt tgacagttat tataatactc
      301 ggggtgcggg gaaaaaacaa ggtgaaattt caccgacgaa aaggggaatt ttccaccatt
      361 taggacagtt tgcccagttg aatcggttcc attttgtgca gcttgcgcag caaaagtgac
      421 aaaatggtta taggcgtttg atttttgtaa gaatttttag gggtaaaaaa gaaaatcctc
      481 cctcgtttac aagggaggta agcccgaagg gccggaggga gttgcgtaag gcagacggtc
      541 gtttatggga aagctgcgga ccgcacatgt gcggccccta cagcacggct gcgttttaat
      601 gcggcaacca tatggaaaac ggcccgggag ggatacatat ttcactcaaa cgggaacaat
      661 gccttttgct tatccatggc gaacacattg tcccgggtga tgatgcgggt ggtggtgacg
      721 gtggattctt ccacgctgct cccctgcccg gccagcagct tgtaggcgct ttcgacgccg
      781 aaatagccca tgccgtaggt attctgcacg atcagcgcgt ccaccacgcc ggtctgtaag
      841 gcgtcgatgg tctcgatgtt cgagtcgaac gccaccagcc aaagatcatc tgcccgctgc
      901 atctgctgca cggcgcgggc tgcgcccacg gcggtggcct cgttgaaggc aaccagcacg
      961 ttgatttcgg ggtgctcacg cagcatggac aaagcctcgc tgcgggcgct gacggcgttg
     1021 gagtgggtcg gcatggcggc gacgacctct gcccggccac tgtccgcaaa agcgtcgcgt
     1081 gcgccctgct cgcggtcgcg gccattggca ccgttgacct cgtagttgat aaggccgacg
     1141 tagagctggc catccatgcc gtccagggcg ctctgggcgg ccatctggcc cgcgccgtag
     1201 ttgtccgcgc cgatgtaggc cgccacatgg gcggagtcca ccgccgagtc aatgccaatg
     1261 atggtcacac ccgccgcggc cgcggcatcg atggcggcag cgttcttttc atagtcgatg
     1321 gcggaaaaga caatggcctg tgcaccgtcc tcgacggcct tctcgatcat gcgatcctgt
     1381 tcctcgtagt cttcctcact gttgggggcc gcgatggtca gctgcatatt gtactcggtg
     1441 gcggcagcct ccgcgccggt aaagacagcg ttccagaact ccgaatcctt ggattttgcc
     1501 actaacgcca cctggtagcg ggtcgcgttg ctgtccgtgg cctgcatggt acagcccgcc
     1561 agcgccgccg ccaaggccag cggcagcaga cgtttaacgc agtttgtcat agtctccctc
     1621 cctgtcctcc ggcacgcgcg gagtgcggat ggttacggtc gtgccctcgt agggggcact
     1681 gacgatggag atgccgtaag ccgcgccgaa gtagatgcgc agccggtcgt tgacgtt
//
LOCUS       DBMDSQ010000395         1728 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_14958_length_1728_cov_1.667486, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000395 DBMDSQ010000000
VERSION     DBMDSQ010000395.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 1728)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1728)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1728
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14958_length_1728_cov_1.667486"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..>1728
                     /gene="uvrA"
                     /locus_tag="ACZPAX_10425"
     CDS             <1..>1728
                     /gene="uvrA"
                     /locus_tag="ACZPAX_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HIR1114350.1"
                     /translation="GLVVIDVIGGEPMMFSQSYACPEHGVSVEELTPRMFSFNNPFGA
                     CEKCTGLGTFMKVDPARVIPDKRKSIRESAIKASGWYYAEGSISEMTYKALGKHYGFT
                     LDTPIKEFSKEALHALLYGTGDERIEMQRESMFGSGTYYNQFEGIIPNLERRFRETSS
                     EWVKEEIALVMSSEECPACHGRRLKPTSLAVTVGGINISDFCDKSVNEELDFINTAKV
                     SARDEMIGAPIFKEVKERLGFLKSVGLGYLTLSRGASSLSGGESQRIRLATQIGSALT
                     GVLYVLDEPSIGLHQRDNDKLIATMKRLRDLGNTLIVVEHDEDTMRQADYIVDVGPGA
                     GVHGGEIVAAGSVADICKAKRSITGAYLSGRKFVEVPATRREGNGKFLRVVKARENNL
                     QDITVDFPLGKMICVTGVSGSGKSTLVNEILYKTLAAALNGARSRPGQCEEVEGMEWV
                     DKVIGIDQSPIGRTPRSNPATYTGVFGDIRSLFANTQDAKMRGYGPGRFSFNVKGGRC
                     EACEGGGILTIEMHFLPDIYVPCDVCKGKRYNRETLEVKYKDKNISDVLDMTVEEACA
                     FFANIPKIAR"
BASE COUNT          370 a          500 c          531 g          327 t
ORIGIN      
        1 cggcttggtg gtcattgacg ttatcggcgg cgaaccgatg atgttcagtc agagctacgc
       61 ctgccccgag cacggcgtct cggtcgagga actgacccct cggatgttca gcttcaacaa
      121 cccattcggt gcctgcgaga aatgcactgg cctgggcacc tttatgaaag tagacccggc
      181 ccgcgtcatc cccgacaagc gcaagtccat ccgtgagagt gccatcaagg ccagcggctg
      241 gtactatgcc gagggttcaa tctcggaaat gacctacaag gccctgggca agcactacgg
      301 cttcacgctg gacacaccca tcaaggagtt cagcaaggag gccctgcacg ccctgctgta
      361 tggtaccgga gatgaacgta ttgagatgca gcgcgagagt atgttcggct cgggcaccta
      421 ctacaaccag tttgagggca tcattccgaa cctggaacgc cgcttccgcg agacgagcag
      481 cgagtgggtc aaggaggaaa tcgcccttgt catgtccagc gaggagtgtc ccgcctgcca
      541 tggccgccgc ttaaagccca ccagcctggc cgtgaccgtg ggcggcatca acatttccga
      601 cttctgcgac aagagcgtca atgaagaact ggatttcatt aacaccgcca aggtcagcgc
      661 ccgggacgaa atgatcggtg cgccgatttt taaagaggtc aaagagcgcc tgggcttctt
      721 gaagagtgtg ggcctgggtt acttgaccct gtcccgcggc gcgtcgtcgc tgtccggcgg
      781 cgagagccag cgcatccgcc tggcgaccca gattggcagt gcgctgactg gtgtgctcta
      841 tgtgctggat gagccgagta tcggcctgca ccagcgtgac aacgacaagc tgattgccac
      901 gatgaagcgc ctgcgcgact tgggcaacac cctgattgtc gtggagcacg acgaggacac
      961 gatgcgtcag gcagactata tcgtcgatgt tggccccggt gcgggcgtcc acggcggtga
     1021 gatcgtcgcg gctggcagtg tagcggatat ctgcaaggca aagcgcagca tcaccggcgc
     1081 gtacttgtcg ggccgcaaat ttgttgaggt tcccgccacc cggcgcgagg gaaacggaaa
     1141 gttcctgcgc gtcgtcaagg cacgcgagaa caacctgcag gatatcacag tagattttcc
     1201 gctgggcaag atgatctgcg tcaccggcgt gtccggctcc ggaaaatcga cgctggtcaa
     1261 tgaaattttg tacaagacgc tggcggcagc cctgaacggt gcccgcagcc gtcccggcca
     1321 gtgcgaggaa gttgagggca tggagtgggt cgataaggtc attggcattg accagtcgcc
     1381 cattggccgc acgccgcgct ccaacccggc cacctacacc ggcgtgttcg gggatatccg
     1441 cagcctgttc gccaacaccc aggacgccaa aatgcgcggc tacggacccg ggcgtttcag
     1501 ctttaatgtt aagggcggcc gctgtgaagc ctgcgagggc ggcggcatcc tgaccattga
     1561 aatgcacttc ctgcccgata tctacgtgcc atgcgatgtc tgcaagggca agcgctacaa
     1621 ccgcgagacg ctggaagtca agtacaagga caagaatatt tccgatgtgt tggatatgac
     1681 ggtggaggaa gcttgcgctt tcttcgccaa catcccgaaa attgcccg
//
LOCUS       DBMDSQ010000396         1725 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_14995_length_1725_cov_1.030172, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000396 DBMDSQ010000000
VERSION     DBMDSQ010000396.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 1725)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1725)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1725
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14995_length_1725_cov_1.030172"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..332
                     /locus_tag="ACZPAX_10430"
     CDS             <1..332
                     /locus_tag="ACZPAX_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013485178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HIR1114351.1"
                     /translation="PCSTCQKAKKWLDDHGIAYTARHIKADNPTADELRAWIAQSGLP
                     IKRFFNTSGLVYKNLGLKDKLPTMTEDEQIALLASDGMLVKRPLLVADDFVLPGFKPA
                     EWEKALL"
     gene            379..>1396
                     /locus_tag="ACZPAX_10435"
     CDS             379..>1396
                     /locus_tag="ACZPAX_10435"
                     /inference="COORDINATES: protein motif:HMM:NF013591.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HIR1114352.1"
                     /translation="MAEILKDRSELDTQFQWDLTPMFENDDAWEATLASLDADIESLS
                     AFAGKLNDAVTIGAYLDASTEVGRKVERLYCYASQRHDEDTRDDKAQSMYARVGSKYV
                     KMAAALSFAQPEILSLPEETLTAIVNDPAVADYKFNLEDLLRSKPHTLTKAEEKLLAS
                     FGEVMSAPSEAYHNLEDADLLFDAVKNGAGETVEVTGSNFVPLEMGTDRVLRENAFRS
                     YYKSYREHINTFAAMYSGAIKAATAEAAARNYPSSRAMAMAGENVPAEVYDNLIATVR
                     KHMPAMHRYVRLRKKMLGVDELHFYDVYAPLVGDLKKSYTYETAQEMVLKAVAPLGED
                     YQETV"
     assembly_gap    1397..1406
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1407..>1725
                     /locus_tag="ACZPAX_10440"
     CDS             <1407..>1725
                     /locus_tag="ACZPAX_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HIR1114353.1"
                     /translation="DVYPNRGKRGGAYSGGCYDSNPYILLNFSGTLDSVSTLAHEMGH
                     TIHSWRSRQHQPPQYADYTLFVAEVASTVNENLLIEQLLQNENDPQTRLYLLNQYLEN
                     FKGT"
BASE COUNT          380 a          575 c          455 g          305 t
ORIGIN      
        1 cgccgtgttc tacctgccaa aaggccaaaa aatggctgga cgaccacggc attgcctaca
       61 ccgcccgcca tatcaaggcg gataatccta ccgccgacga gctgcgtgcc tggattgccc
      121 aaagcggcct gcccatcaag cggttcttca acaccagcgg ccttgtctac aaaaatttgg
      181 gtctgaaaga caagctgccc accatgaccg aggacgagca gatcgccctg ctggcatcgg
      241 acggtatgct cgtcaagcgc cccctgctgg tggcggacga cttcgtcctg cccggcttca
      301 aacccgccga atgggagaag gcgcttttgt aaacgcatat cctctgttct gcaaaaccat
      361 attaataagg agaaaaccat ggctgaaatt ttaaaagacc gcagcgaact cgacacgcag
      421 ttccagtggg acctgacccc gatgtttgag aatgacgacg cctgggaagc caccctcgcc
      481 agcctcgacg cggatattga gagtctgtcc gcctttgctg gcaaactgaa cgatgccgtt
      541 accatcggtg cgtacctcga tgcctccacc gaggtgggcc gcaaggtcga gcgcctgtac
      601 tgctatgcct cccagcgcca cgacgaggac acccgcgacg acaaggctca gagtatgtat
      661 gcccgcgtcg gcagcaagta cgtcaagatg gctgccgccc tgtccttcgc ccagcccgaa
      721 atcctgtccc tgcccgagga gacgctgacc gccatcgtca acgaccctgc cgtcgccgac
      781 tacaagttca acctcgagga tctgctgcgc agcaaacccc acaccctgac caaggctgag
      841 gaaaagctgc tggccagctt tggcgaggtc atgtccgcgc cctccgaggc ctaccacaat
      901 ttggaggatg ccgacctgct gtttgatgcc gtcaagaacg gcgcaggaga gactgtcgag
      961 gtcaccggct ccaactttgt gcctctggaa atgggtaccg accgtgttct gcgcgagaat
     1021 gctttccgca gctactacaa gagctaccgc gagcacatca acaccttcgc ggccatgtac
     1081 agcggcgcta tcaaggccgc caccgccgag gctgctgccc gcaattatcc gtcctcccgc
     1141 gccatggcca tggcagggga gaacgtgccc gccgaggtct atgacaacct cattgcgacc
     1201 gtccgcaagc atatgcccgc catgcaccgc tatgtccgcc tgcgcaaaaa gatgttgggc
     1261 gttgatgagc tgcatttcta cgacgtctac gccccgctgg tcggcgacct gaaaaaatcc
     1321 tacacctacg agaccgcgca ggaaatggtg ctgaaagccg ttgcccccct gggcgaagac
     1381 taccaggaaa ctgtccnnnn nnnnnntcga cgtttacccc aaccgcggca agcgcggcgg
     1441 tgcctactcc ggcggctgct atgactccaa cccctatatc ctgctgaatt tcagcggcac
     1501 gctggacagc gtctccaccc tggcgcacga gatgggccac accatccaca gctggcgatc
     1561 ccgccagcac cagccgccgc agtacgcgga ctatacgctg ttcgtcgctg aggtcgcgtc
     1621 caccgtcaac gagaacctgc tcattgagca gctgctccag aacgagaacg acccccagac
     1681 ccgcctgtac ctgctgaacc agtacctcga gaacttcaag ggcac
//
LOCUS       DBMDSQ010000397         1724 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_14999_length_1724_cov_1.670980, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000397 DBMDSQ010000000
VERSION     DBMDSQ010000397.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 1724)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1724)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1724
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14999_length_1724_cov_1.670980"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            408..1370
                     /locus_tag="ACZPAX_10445"
     CDS             408..1370
                     /locus_tag="ACZPAX_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005922631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HIR1114354.1"
                     /translation="MLIPVLLFIVGLLFLIKGGDWFVDGASALARRFHLPELLIGATV
                     VSIGTTLPEVMVSTMSALSGHGEIAYGNAIGSVICNAALIAAITIAVRPGKVDPKTLK
                     TPVAFFFAAAAIYCIAAYVFGKFTRVMGFIMLAMFVAYMVVNVLQMKKAPVVDAETSE
                     EEEMSLARTLILLVLGAVLIAVGANLLVDNGTLIAQALGVPESVIALTFVALGTSLPE
                     LVTAITSLAKGHSDLSLGNVVGANVFNLVLVSGVSVSLAPFTIPQNSTLFGINSSLVL
                     ELPVMLAVMLILTVPALVKGKLNRGQGILLLAIYAAFCAVQFTL"
     gene            1392..1571
                     /locus_tag="ACZPAX_10450"
     CDS             1392..1571
                     /locus_tag="ACZPAX_10450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1114355.1"
                     /translation="MNLQELSAAINTLGAFCGPRGKNFLAHVEIPAEVQAAFAQLQTV
                     YDTDTRAANPAYATK"
BASE COUNT          316 a          537 c          480 g          391 t
ORIGIN      
        1 cccctctatg cacaaaggcc cgcaggtatc ccctgcgggc ctttgtgttt gtacagatag
       61 gacagatgcg ggcaatgtgt gcaaaatatg gagcaaacta aagccttccc ctcgggggaa
      121 ggtgacccaa agggctgaat gaggggcgcg tgtaccgcta ttgcccgcaa atgggttgct
      181 gctggaggtt tgcccctcct cagtccgctt cgcgggcagc ttcagtctcc gcgctaagag
      241 ccgccttcgg cggttacgct ccgacacgcg cctgcgggcg cagcccctcg gagggaagcc
      301 tttgcaaaat ttacaattta aactccaccg catttgacgc agcaccgttc tctatgctat
      361 aataaaaatt ccgacgtcac tcgtcatttt ttacggagga atcacctatg cttatccctg
      421 tactgctgtt tatcgtcggt ctgctgttcc ttattaaagg cggtgactgg tttgttgacg
      481 gtgcttctgc gctggcccgg cggttccacc tgccggaact gctcatcggt gcgaccgtcg
      541 tttccatcgg tacgactttg ccggaggtca tggtctccac catgtccgcg ctgtccggcc
      601 atggcgagat cgcctacggc aacgccatcg gctcggtcat ctgcaacgcg gcgctcattg
      661 cggctatcac catcgccgtg cgccccggca aggtcgaccc caaaacgctg aaaaccccgg
      721 tcgcattctt ctttgcggcg gcggccatct actgcattgc ggcgtatgtt ttcggcaagt
      781 tcacccgcgt catgggcttt atcatgctgg ctatgttcgt ggcctacatg gtcgtcaacg
      841 tgctgcagat gaaaaaagcc cccgtcgttg acgctgagac gtccgaagaa gaggaaatgt
      901 ccctggccag gacgctgatc ctgctggtgc tgggcgcggt gctgatcgcc gtgggcgcaa
      961 acctgctggt ggacaacggt accctgattg cccaggccct cggtgtgccg gagagcgtca
     1021 ttgcactgac ctttgtcgcg ctgggtacct ctctgccaga gctggtcaca gccatcacct
     1081 cgctggccaa gggccacagc gatctgtcgc tgggcaacgt cgtcggcgca aacgtgttca
     1141 accttgtgct ggtcagcggc gtgtccgtct cgctggcacc cttcaccatc ccgcagaact
     1201 ccacgctgtt cggtatcaat tccagccttg tgttggaact gccggtcatg ctggccgtca
     1261 tgctgattct gaccgtgccc gccctcgtca agggcaagct aaaccgcggc cagggtatcc
     1321 tgttgctggc catctacgcc gccttttgcg cagtgcagtt tacgctgtaa tatagtagaa
     1381 ggtagttccc tatgaacctg caagaacttt ccgccgccat caacacgctg ggggcgttct
     1441 gcggcccgcg gggtaaaaac ttcctggccc atgtggagat ccctgccgag gtgcaggcgg
     1501 cctttgccca gctgcaaacc gtctacgaca ccgacacccg cgccgccaac ccggcctacg
     1561 ccacaaaata aattttgccc aaaatgaaac gccctgccgc ggtacacacg gcagggcgtt
     1621 tggtttttcc ggcacacggc gtgccggggg tcatatttgc ctgcgcttgc ggcgcaatac
     1681 cattgcggcg gcaaggcttc ttcacaatac tccctctgat agca
//
LOCUS       DBMDSQ010000398         1718 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_15085_length_1718_cov_2.150897, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000398 DBMDSQ010000000
VERSION     DBMDSQ010000398.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 1718)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1718)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1718
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15085_length_1718_cov_2.150897"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            281..1246
                     /locus_tag="ACZPAX_10455"
     CDS             281..1246
                     /locus_tag="ACZPAX_10455"
                     /inference="COORDINATES: protein motif:HMM:NF025616.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HIR1114356.1"
                     /translation="MKKRITRITAALAAAALLAGCGSISSSDGGYYATEAAAQESYNY
                     DSAAGAVDSSLMPANAAEAATDETAQKIIYNASLSMESTDFDAARETLMAAVEANNAW
                     MESTSVYGTEKDHDRSADYTVRVPVDNYRAFLAAVGEAGSVRNVSENAQNITSSYIDV
                     ESRLAALEAQRTRLNELADQAETTADLLEIESQLSDVQYQLENYTRQLRNMDQQVSYS
                     TVDIYLEEVATLTPTGVTFTERIADAFGGGWDAFVGFVQGLVIALVYLWPVVLIVIAV
                     IAALRFWRKRHPKALKAPKAPKPIQTPPPAAEYPVKQDEEPKPKY"
     gene            complement(1413..>1718)
                     /locus_tag="ACZPAX_10460"
     CDS             complement(1413..>1718)
                     /locus_tag="ACZPAX_10460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HIR1114357.1"
                     /translation="SMRINDPALQDRMFHALGFTEEKARESFGFLMDAYKFGAPPHGG
                     MAYGLDRMVMLMLKKDSIRDVIAFPKVQNAGEPMSGAPDVVDEQQLKDLSIALTLKD"
BASE COUNT          385 a          530 c          502 g          301 t
ORIGIN      
        1 gccgaataag caaccaaaaa tttgtgggat gtgccaaaat tgcggcacat cccacttttt
       61 tgtatttggt ttgtaacaca cagctggcat tttccgtgtt atattggtga aaagggaaat
      121 gcctttcccc cgagggggaa ggtggccgaa ggccggatga ggggcggcgt tcccgacacc
      181 gagccgtaaa aaggcggcaa gacaaacttg cccccatcag tccgcttcgc gaacagcttt
      241 cccctcaggg gaagccttta cgtagaaagg agcatccaac atgaaaaagc gcatcacccg
      301 tatcaccgct gcgctggccg ctgctgcact gctggcgggc tgcggcagca tcagcagcag
      361 cgacggcggc tactacgcca ccgaggccgc cgcgcaggaa agctacaatt atgacagcgc
      421 cgcaggcgcg gtagacagca gcctgatgcc cgccaacgcc gccgaagccg ctaccgatga
      481 gaccgcgcag aaaatcatct acaacgccag cctgtcgatg gaatccaccg actttgacgc
      541 cgcccgcgaa accctgatgg ctgcggtcga ggccaacaat gcatggatgg agtccaccag
      601 cgtttacggc actgaaaagg atcacgaccg cagcgccgac tacaccgtgc gggtgccggt
      661 ggacaactac cgcgcgtttt tggctgccgt gggcgaggca ggcagcgtgc gcaatgtcag
      721 cgagaatgcc cagaacatca ccagcagcta cattgatgtg gaatcccggc tggcggcact
      781 ggaagcccag cgcacccgcc tgaatgagct ggcagaccag gccgagacga ccgccgacct
      841 gctggaaatc gagagccagc tttccgatgt gcagtaccag ttggagaact acacccgcca
      901 gctgcgcaat atggaccagc aggtcagcta ctcgaccgta gacatctatc tggaagaggt
      961 cgcaaccctg accccgaccg gcgtgacctt caccgagcgc attgccgatg cgttcggcgg
     1021 cggctgggac gccttcgttg ggtttgtgca ggggctggtc attgcgctgg tctacctgtg
     1081 gccagtggtg ctgatcgtca ttgcagtcat tgcggctctg cgattctggc gcaagcgcca
     1141 tccgaaagcc ctcaaggccc ccaaagcgcc caagcccatc cagaccccgc cgccagctgc
     1201 ggagtacccg gtaaagcagg acgaggaacc gaagccgaaa tattgagata acacaaaaac
     1261 gcccgaccgc ataaaatacg gtcgggcgta tattttttct atcctgtagg ggcgaggcat
     1321 gcctcgccct gcacctaccc gcaaaaggga atcctgcggg taggccgcgg gccgggcatg
     1381 cccggtccct acacagcaac caaattttca ggttagtcct tcagcgtcag cgcgatgctc
     1441 aaatccttga gctgctgctc gtccaccacg tccggtgcgc cgctcatcgg ctcgccggca
     1501 ttctgcacct tcgggaaggc aatgacgtcg cggatggaat ccttcttcag catcagcatg
     1561 accatacggt ccaggccgta ggccataccg ccgtgcgggg gggcgccgaa cttgtaggcg
     1621 tccatcagga agccaaagct ctcgcgggcc ttctcctcgg tgaatcccag ggcatggaac
     1681 atacgatcct gcaaagcggg gtcgttgata cgcatgga
//
LOCUS       DBMDSQ010000399         1717 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_15099_length_1717_cov_1.528465, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000399 DBMDSQ010000000
VERSION     DBMDSQ010000399.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1717)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15099_length_1717_cov_1.528465"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1569)
                     /locus_tag="ACZPAX_10465"
     CDS             complement(<1..1569)
                     /locus_tag="ACZPAX_10465"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing sensor histidine kinase"
                     /protein_id="HIR1114358.1"
                     /translation="MKRPPRTAPLAAALISAVAVAVLTLCTTLFLRSYRSAVVEAART
                     NSAQAVSQVVGAVGNYVSTMDSAVAIVMERLDDDTASRDAFLSAFLTARPEVAAITAY
                     DADGQLLDCWAQEGRKPKDAILRNLSFDLTTARRLGHGYISTPHVESIFEGYYPWVVS
                     VIRIVPEGDTARCWLSVDLSFKELAATISNVGIGQHGYCYLMDERGNMVYHPQQQLLY
                     AGLKKEEAGRLACLGDGTYVEDTVIYSVQRVPGSPWRVVGVSYIDETVNESFWQMARI
                     AVATAVLILFAALIVGWVLSRSLSRPLQQLETAMEQFEQDADHFAFQSVRGTREVQNL
                     SDSFGHMVGRIQRLMTTVREEEVVLRKTELKALQAQINPHFLYNTLDSIAWMCERGKN
                     ADAVQMVHALARLFRISISRGHELIPIEKELQHAEAYLQIQKYRYKNQFTYHFTVDES
                     CLHCLCNKITLQPIIENAIVHGLDLMVDSGHIEITVKPDGDDILLIVADDGIGMEPEQ
                     VAALLQNEPSDRTGI"
     gene            complement(1566..1715)
                     /locus_tag="ACZPAX_10470"
     CDS             complement(1566..1715)
                     /locus_tag="ACZPAX_10470"
                     /inference="COORDINATES: protein motif:HMM:NF024242.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HIR1114359.1"
                     /translation="MLTTDLQIQEIARRCGYTDQHYFSYCFKKYSGTSPVALRRARAA
                     GEVHA"
BASE COUNT          328 a          513 c          545 g          331 t
ORIGIN      
        1 gatgccggtg cggtcgctgg gttcgttctg caaaagtgcc gccacctgct ccggctccat
       61 gccgatgccg tcgtccgcca caatgagcag aatatcgtca ccgtcgggct tgaccgtgat
      121 ctcaatatgg ccggaatcga ccatcaaatc caggccgtgg acaatggcgt tttcgatgat
      181 gggctgcagg gtgattttgt tgcacagaca gtgcaggcag ctctcgtcca ccgtgaaatg
      241 ataggtaaac tggttcttat agcggtattt ttggatttgc aagtaggctt cggcgtgctg
      301 cagttctttt tcaatcggga tcagttcgtg cccacggctg atgctgatgc ggaagagccg
      361 cgccagcgcg tggaccatct ggacggcgtc ggcatttttg ccgcgttcgc acatccaggc
      421 gatggaatcg agcgtattgt agaggaaatg tgggttgatc tgagcctgca gcgccttgag
      481 ttcggtcttg cgcagcacaa cttcctcctc gcgcacggtg gtcatcagcc gctggatacg
      541 gcccaccata tgtccgaagg aatccgagag gttctgcacc tcccgcgtgc cgcgcaccga
      601 ctggaacgca aagtggtcgg cgtcctgctc aaactgctcc atggcggttt ccagctgctg
      661 caaggggcgg gacagcgagc gggacaacac ccagcccacg atgagcgccg caaacaggat
      721 caatacggcg gtagccacag cgatgcgggc catctgccag aagctctcgt tgacggtctc
      781 gtcgatgtag ctgacaccga cgacgcgcca cgggctgccc ggcacgcgct ggacgctgta
      841 aatgacggta tcctcgacat aggtgccgtc gccaaggcag gccagacggc ccgcttcctc
      901 ctttttcagg cccgcgtaca aaagctgctg ctgcgggtgg tagaccatgt tgccgcgttc
      961 gtccatcagg tagcagtagc cgtgctggcc aatgccaacg ttgctgatgg tggcagccag
     1021 ctccttgaag ctcaaatcca ccgagagcca gcaccgcgca gtgtcacctt ccggcacaat
     1081 acggatgacc gacacgaccc aggggtagta accttcaaaa atcgactcga cgtggggcgt
     1141 gctgatgtag ccgtggccca ggcggcgggc ggtggtcaaa tcaaaggaaa ggttccgcaa
     1201 aatcgcatcc ttgggtttgc ggccctcctg cgcccagcag tccagcagct ggccgtctgc
     1261 atcgtaggcg gtgatggctg cgacctcggg ccgggcggtg aggaacgcgc tcaaaaaggc
     1321 gtcgcggctg gcggtgtcgt cgtccagccg ctccatgacg atggcgacgg cgctgtccat
     1381 tgtgctgaca tagttgccca cggcccccac gacctggctg acggcctggg cactgttcgt
     1441 gcgggcggcc tcgaccacgg cgctgcggta gctgcgcaga aacagcgtcg tgcagagcgt
     1501 cagcaccgcc accgccacgg cagaaatcag cgcggcggcc aggggcgcag tgcggggcgg
     1561 gcgcttcatg cgtggacctc ccccgctgcg cgggcgcggc gcagggccac cggcgacgta
     1621 ccgctgtact ttttaaagca atagctaaag tagtgctggt ctgtatagcc gcagcggcgg
     1681 gcgatttcct gtatctgcaa atcagtggta agcaaaa
//
LOCUS       DBMDSQ010000400         1717 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_15100_length_1717_cov_1.460396, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000400 DBMDSQ010000000
VERSION     DBMDSQ010000400.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1717)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15100_length_1717_cov_1.460396"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1089
                     /gene="pepT"
                     /locus_tag="ACZPAX_10475"
     CDS             <1..1089
                     /gene="pepT"
                     /locus_tag="ACZPAX_10475"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012058642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HIR1114360.1"
                     /translation="ELGLTDAHVDEHCYVYATLPATPGQENAKPLGFIAHMDTADDAS
                     GENVQPQIHENYDGEAVVLPATGTVLDPEVFPFLRTMKGQTLITTDGSTLLGADDKAG
                     VAEIMTALERIIAENRPHGKLCIGFTPDEEIGEGASLFDVPGFGAAYAYTVDGEDVGE
                     ISYENFNAAAAVVTVHGFSVHPGSAKDTMINAQNVAMEFHAALPAFSRPEHTEGREGF
                     FHLTSMQGDVTTAHLGYIVRDHDAAKFAARKAQMQHIADCLNGRYGAGTVELDIKDSY
                     YNMLEKIQPHFHLVENARKAIRAAGLEPIESPVRGGTDGATLSYMGLPCPNLGTGGFN
                     FHGPCECITAEKMDQGVEILLNLVDLYK"
     gene            1236..>1717
                     /locus_tag="ACZPAX_10480"
     CDS             1236..>1717
                     /locus_tag="ACZPAX_10480"
                     /inference="COORDINATES: protein motif:HMM:NF044704.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009303 - rRNA transcription [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HIR1114361.1"
                     /translation="MFKAGDTVSYGTSGVCTIAEQKRVTLGGKLHDCFILKPVYDASM
                     KICVPCDSAPLLERMRPLPTRDDLLALLREPLPDHDPDPDARKLAYREALKSGNLHAL
                     VRMVRDIRTEQALRRAKGKQLSAYEDSALRDAQNVLHSEFAYVMGIEPNEVPKFIAKE
                     "
BASE COUNT          377 a          541 c          504 g          295 t
ORIGIN      
        1 gaactgggcc tgaccgatgc ccatgtggac gagcactgct atgtctacgc cacgctgccc
       61 gccaccccgg ggcaggagaa cgccaagccc ctgggcttta tcgcccacat ggacacggcg
      121 gacgacgcca gcggtgagaa tgtccagccg cagatccacg agaattacga cggtgaggcc
      181 gtcgtcctgc ctgccaccgg cacggtgctg gacccagagg ttttcccctt cctgcgcacg
      241 atgaagggcc agaccctcat caccaccgac ggctccacgc tgctgggtgc ggacgataag
      301 gccggcgtgg ccgagatcat gactgcattg gagcgcatca ttgccgaaaa ccgcccccac
      361 ggcaagctct gcatcggctt cacccccgat gaggagatcg gcgagggcgc gagcctcttt
      421 gacgtgcccg gcttcggcgc ggcctacgcc tacaccgttg atggcgagga cgtcggcgag
      481 atcagctatg agaacttcaa cgccgccgcc gcggtcgtga ccgtccacgg tttcagcgtc
      541 caccccggca gtgccaagga cacgatgatc aacgcccaga atgtggcgat ggagttccac
      601 gccgcattgc cggccttctc ccgccccgag cacaccgagg gaagggaagg cttcttccac
      661 ctgacgagta tgcaggggga tgtgaccaca gcgcatctgg gctacatcgt gcgcgaccac
      721 gatgccgcca agtttgccgc ccgcaaagcc cagatgcagc acatcgccga ctgcctgaac
      781 ggccgttacg gcgcgggcac ggtggaactg gacatcaagg acagctatta caatatgctg
      841 gagaagattc agccccactt ccacctggtg gagaatgccc gcaaggccat ccgtgcggcg
      901 gggctggaac ccatcgaaag cccggtgcgc ggcggcactg acggcgcgac cctgagctat
      961 atgggcctgc cctgcccgaa cctcggcaca ggcggcttca acttccacgg cccgtgtgag
     1021 tgcatcacag ccgaaaagat ggaccagggc gtggaaattc tgctgaacct ggtcgatctg
     1081 tataagtaaa acctaacccc ggcgcaatct cgtacatttt gtacaaaact gcgccggggc
     1141 atttttgtcc aaatcgccta tgacattttt tgacgaaaag gcgtatactt gcataggaaa
     1201 gaaatcattt catgagagga aaaggagtat gcgtcatgtt taaagcaggg gatacggtca
     1261 gctatggcac cagcggcgtc tgcacgattg ccgagcaaaa gcgtgtcacg ctgggcggca
     1321 agctccacga ttgttttatc ttaaaaccgg tctacgacgc cagtatgaaa atctgtgtgc
     1381 cctgcgacag tgcaccgctt ttggagcgaa tgcgcccatt gcccacccgg gacgacctgc
     1441 tggccctgct gcgggaaccg ttgcccgacc acgacccgga ccccgacgcc cgcaagctgg
     1501 cctatcgcga ggcactgaaa agcggcaatc tgcacgcgct ggtgcgcatg gtgcgggata
     1561 tccgcaccga acaagccctg cgccgcgcca agggcaagca gctttccgcc tatgaggaca
     1621 gcgccctgcg cgacgcccag aacgtcctgc acagtgagtt cgcctatgtc atgggcatag
     1681 aaccaaacga agtgccgaag ttcattgcaa aagaatt
//
LOCUS       DBMDSQ010000401         1711 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_15161_length_1711_cov_1.234783, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000401 DBMDSQ010000000
VERSION     DBMDSQ010000401.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 1711)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1711)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1711
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15161_length_1711_cov_1.234783"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..463)
                     /locus_tag="ACZPAX_10485"
     CDS             complement(<1..463)
                     /locus_tag="ACZPAX_10485"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007048262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HIR1114362.1"
                     /translation="MSKIFFHVDVNSAFLSWSAIKHLRDGEDVDLRTIPAVVGGDEAQ
                     RHGVVLAKSGPAKKYGIKTGESLFAARTKYPGIKVVPPDFDFYVQKSKAMIAILQDYT
                     PDVEQYSIDEAFLDMTGTEQLFGPPLTVAHAIRQRIYRELGFTVNVGVAPNR"
     gene            769..>1314
                     /locus_tag="ACZPAX_10490"
     CDS             769..>1314
                     /locus_tag="ACZPAX_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005920545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rossmann-fold NAD(P)-binding domain-containing
                     protein"
                     /protein_id="HIR1114363.1"
                     /translation="MDLIGRDFLTLLDFTPAEIGSLLDLAAELKAKKKAGIPHDTLRG
                     KNVALIFEKTSTRTRCSFEVAAHDLGMGTTYLDPTGSQIGKKESIADTAQVLSGMFEG
                     IEYRGYGQAIVEELARYASVPVWNGLTNEYHPTQILADFLTLREHFGKLQGLKFAYMG
                     DARYNMGNSLMIGCAKMGLDFV"
     assembly_gap    1315..1324
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1325..>1711
                     /locus_tag="ACZPAX_10495"
     CDS             <1325..>1711
                     /locus_tag="ACZPAX_10495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012058660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Rossmann-fold NAD(P)-binding domain-containing
                     protein"
                     /protein_id="HIR1114364.1"
                     /translation="WPDPALVEKCKEFAERSGASITLSEDTDAVAGADVIYTDVWVSM
                     GEPVEVWAERIEALSPYQVNAELMFKAKPSAVFMHCLPAYHDHKTAVGKEMGEKFNRD
                     AMEVTDEVFNGPQSIVFQEAENRMHT"
BASE COUNT          345 a          540 c          500 g          316 t
ORIGIN      
        1 ggcggttggg cgctacgccc acgttgacgg tgaaccccag ctccctataa atgcgctggc
       61 ggatggcgtg ggccacagtc agcggcgggc cgaagagctg ctcggtgccg gtcatgtcca
      121 ggaaggcttc gtcgatgctg tactgctcca catcgggcgt ataatcttgc aaaatcgcga
      181 tcatcgcctt gctcttctgc acgtaaaagt cgaagtccgg cggcaccacc ttgatgcccg
      241 ggtacttggt gcgggcggca aacaggcttt cgccggtttt gatgccgtat ttcttggcgg
      301 ggccgctctt ggccagcacg acgccatggc gctgggcttc gtcgccgccc accaccgccg
      361 ggatggtgcg caggtccacg tcctcgccgt cccgcagatg cttgatggcc gaccaagaca
      421 aaaacgccga gttgacatcc acatggaaaa agattttgga catgggcacc tcctttcccg
      481 gttataaaag gctccccttg aggggtaact ccccgcagtg cggggagatg tcgcgcagcg
      541 acagaggggc ccggccgcgt aagcgctggc ggcgcagccg actgaggggt ggcccacgtg
      601 ccgctcgttg ctaaagtagc cacccctcac ccgcttcgcg ggagctcccc tctaggggag
      661 ccatccttta ctttttatta taccacttgc aaccccacac ggaaagtggt ataatagcat
      721 gtataattat tcaattatac agtatatatt taaaggaggt tccccaccat ggacctgatc
      781 ggacgcgact ttttaacgct gctggacttt accccggccg agatcggcag cctgctggac
      841 ctggctgctg agctgaaagc caaaaagaag gccggcatcc cccacgatac gctgcgcggc
      901 aaaaacgtgg cgctcatttt tgagaaaact tccacccgca cccgctgcag ctttgaggtt
      961 gccgcccacg atctgggcat gggcaccacc tacctggacc ccaccggcag ccagatcggc
     1021 aaaaaggagt ccatcgccga caccgcccag gtgctttccg gcatgtttga gggcatcgag
     1081 taccgcggct acggccaggc catcgtggag gagctggcca ggtatgccag cgtgcccgtc
     1141 tggaacggcc tgaccaacga gtaccatccc acccagatcc tggccgactt cctcaccctg
     1201 cgggagcatt tcggcaagct gcagggcctc aagtttgcct acatgggcga cgcccgctac
     1261 aacatgggca acagcctgat gatcggctgc gccaagatgg gcctggattt cgtgnnnnnn
     1321 nnnnctggcc tgaccccgcc ctggtggaaa agtgcaaaga gtttgccgag cgcagcggcg
     1381 cctccatcac cctgtccgag gacaccgacg ctgtggccgg tgcggatgtc atctacaccg
     1441 acgtgtgggt cagcatgggc gagccggtgg aagtctgggc cgagcgcatt gaggccctgt
     1501 ccccctacca ggtcaacgcc gaactgatgt tcaaggccaa gcccagcgcc gtgttcatgc
     1561 actgcctgcc cgcctaccac gaccacaaga ccgccgtggg caaggagatg ggcgagaagt
     1621 tcaaccgcga cgccatggaa gtgaccgacg aggtcttcaa cggcccgcag tccatcgtct
     1681 tccaggaagc cgagaaccgc atgcacacca t
//
LOCUS       DBMDSQ010000402         1705 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_15224_length_1705_cov_1.859102, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000402 DBMDSQ010000000
VERSION     DBMDSQ010000402.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 1705)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1705)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1705
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15224_length_1705_cov_1.859102"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..525
                     /locus_tag="ACZPAX_10500"
     CDS             <1..525
                     /locus_tag="ACZPAX_10500"
                     /inference="COORDINATES: protein motif:HMM:NF016296.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HIR1114365.1"
                     /translation="DPAGLAYLAAVRTAGREAFLLVMAAATTSPLVYLKPELAPDSLI
                     LRPVTAEECRRAAGEIFDRLLAAQHEGADCFVVKSRDERIRVPYRKILYFEARERRLC
                     LRMAGEELTFTGTLEKLLETLPPEFRRVHRSFVVNADNIQRVVLSQNLVYLRGDVAVP
                     LSRSYKKEVREYDA"
     gene            515..1204
                     /locus_tag="ACZPAX_10505"
     CDS             515..1204
                     /locus_tag="ACZPAX_10505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1114366.1"
                     /translation="MTHDENTAMERSYHPTGAELWFLLSLLDDRPAPLFWPMGEPAEN
                     WPLVAVRLLDAGLAVRGENGLTPAAPLAPLLDTVKSADRAALVFLRTPGGAVDAVLYY
                     NRVGGVLLRRESAARYTLQALDTAALLAALPPLNGAGEGTLYPEFIPPADLPPLDADG
                     TPCREDAATAALVELFAPDCTRLARWQWLDTGCGCKVRRQTPDSVTTAPDGPDIRASL
                     AAALTGEDAPC"
     gene            1198..1443
                     /locus_tag="ACZPAX_10510"
     CDS             1198..1443
                     /locus_tag="ACZPAX_10510"
                     /inference="COORDINATES: protein motif:HMM:NF020396.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EsaB/YukD family protein"
                     /protein_id="HIR1114367.1"
                     /translation="MLNVEIRLPGTGQHYDFALDETAPVAAVTRAVAEAICAETHAPA
                     PGDAPLSLYDPARAARLDPCGTLPENGVGEGAVLILL"
BASE COUNT          274 a          581 c          556 g          294 t
ORIGIN      
        1 gaccctgcag ggctggctta cctggccgct gtgcgcacag ccgggcgcga ggcgtttttg
       61 ctcgtcatgg cggcggccac gacatcgccg ctcgtctacc tgaagcccga gcttgcgccg
      121 gattcactga ttttgcgccc ggtcacggcc gaggaatgcc gccgcgctgc gggggaaatc
      181 ttcgaccggc tgctggccgc ccagcacgag ggggcggact gcttcgtcgt gaaaagccgc
      241 gacgagcgca tccgcgtacc ctaccgcaaa attttgtatt ttgaggcccg ggagcggcgg
      301 ctctgcctgc ggatggcggg cgaggagctg actttcaccg gcacgctgga aaagctgctg
      361 gaaactttac cgccggagtt ccgccgcgtc caccgcagct ttgtggtgaa cgccgacaat
      421 atccagcgcg tagtgttaag ccaaaatttg gtctatctgc gcggggatgt ggctgtgccg
      481 ctctcccgca gctacaaaaa ggaggtgcgg gagtatgacg catgacgaaa atacagcgat
      541 ggaacgttcc tatcacccta cgggggccga gctgtggttt ctgctctcgc tgctggacga
      601 ccgtcccgcg ccgttgttct ggccgatggg tgaaccggcg gaaaactggc cgctcgtcgc
      661 cgtgcggctg ctggacgccg gccttgccgt gcgcggcgaa aacggcctta cccccgccgc
      721 gccgctggcc ccgctgctgg acaccgtgaa atccgccgac cgcgccgcac tggtctttct
      781 gcgcacaccg ggcggggccg ttgacgcggt gctctattat aatagggtag gcggcgtgct
      841 gctgcggcgc gagagcgcgg cccgctacac gctgcaagcc ctggacaccg ctgcgctgct
      901 ggccgccctg ccgccgctga acggtgcggg ggaggggacg ctgtaccccg agttcatccc
      961 gcccgcagac ctgccgccgc tggatgccga cggcaccccc tgccgggagg acgccgccac
     1021 cgccgcgctg gtggagctgt ttgcccccga ctgcacgcgg ctggcccgct ggcaatggct
     1081 ggacaccggc tgcggctgca aggtacgccg ccagacgccc gacagcgtga ccaccgcccc
     1141 cgacggtccg gacatccgcg cctcgctggc tgccgccctg accggggagg acgcgccatg
     1201 ctgaatgtag agatccgcct gcccggcacc gggcagcact acgattttgc cctggacgag
     1261 acggccccgg tggctgccgt gacccgcgcc gtggccgagg ctatctgcgc cgagacgcac
     1321 gcccccgccc ccggggacgc gccgctttcc ctctacgacc ccgcccgcgc ggcgcggctt
     1381 gacccctgcg gcaccctgcc cgaaaacgga gtaggggagg gcgcggtgtt gattttgttg
     1441 taactccttg gcccctgcgt ttacgggtcg gggcttgcat tgacccgcag gcgccgcacc
     1501 cacaccatac tacacgacct gccattcacg caggttcacc gaccattcat cccggttggg
     1561 ctgcacgcgc ggcccgcttg cgctatactg ggatgcagga aaggaaatca ctcaaaagca
     1621 attagctgca aggtcaggtg taagtcttgg ttcacttaag cgatttgagc agagcggcga
     1681 gatatccctg caatctttga ccaaa
//
LOCUS       DBMDSQ010000403         1701 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_15271_length_1701_cov_1.611250, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000403 DBMDSQ010000000
VERSION     DBMDSQ010000403.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 1701)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1701)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1701
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15271_length_1701_cov_1.611250"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..>1701
                     /locus_tag="ACZPAX_10515"
     CDS             <1..>1701
                     /locus_tag="ACZPAX_10515"
                     /inference="COORDINATES: protein motif:HMM:NF012789.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HIR1114368.1"
                     /translation="IMTFTNAAAAKLRADITARLAQEVRAHPGNMNLRRQQLLLQRAS
                     IGTVDAFCLHFVQQHFAMLDVPPDFETAEEADLARIEQETLAATLETAYTDPDFRAFA
                     DLYDRGRTDNTAGDAILDLYHFTRALPHPAASLNTFAEMWQQDAPPQETAWGKELLGI
                     ALARAQGAKTLLESGAAIAARDEKADAAYTAVMQDDAARVGNLCHWLAEGDWDKCLAA
                     LDTVFADWRRAGAVKGGKDANQAASAASELRDRAKKQMESLRKDALLCTADEFAADRR
                     RAAPLVAALVWATQTFADNCFAAKCAEKVLDYADYEHLTLDLLLTEDNERTPLCRTVS
                     ARYAAVLVDEYQDTNALQDAIYFALARPEADNLFFVGDIKQSIYRFRLADPSIFVGKQ
                     QQWRPYPQPMPKPATLALDANFRSAPGVIEGINYLFETFFSEGLGGVQYGDGQRLVVG
                     RKDSDYRGMCEIDVIDGADVDGDAAAIARRIAEMVETGFAVRGKDGVRPCKYDDFCIL
                     LRGRKGFAAYEGALRTAGIPVFADSATDLLDEPHIRPFAALLRVIDNPAQDIPLAAVL
                     "
BASE COUNT          298 a          574 c          545 g          284 t
ORIGIN      
        1 tcatcatgac cttcacgaat gccgccgcgg ccaagctgcg ggcggatatc acggcccgcc
       61 ttgcccaaga ggtccgcgcc caccccggca acatgaatct gcgccgccag cagctgctgt
      121 tgcagcgggc gtccatcggc acggtggacg ccttctgcct gcattttgtg cagcagcatt
      181 tcgccatgct ggatgtgccg cccgactttg agaccgccga ggaagccgac ctggcccgca
      241 ttgagcagga aacgctggct gccacgctgg agaccgccta caccgacccg gatttccgcg
      301 cctttgccga cctgtacgac cggggccgta ccgacaacac ggccggggac gccatcctcg
      361 acctctacca ctttacccgc gccctgccgc atccggccgc gtcgctgaat acttttgccg
      421 aaatgtggca gcaggacgcc ccgccccagg agaccgcctg gggcaaagaa ctgttgggca
      481 ttgcgctggc ccgcgcccag ggcgcaaaaa cgctgctgga aagcggtgcg gccatcgcag
      541 cccgggacga aaaagccgat gctgcctaca cagccgtcat gcaggacgac gcggcccggg
      601 tgggcaatct ctgccactgg ctggccgaag gcgactggga caagtgcctg gccgcgctgg
      661 acacggtctt tgcggactgg cggcgggccg gggcggtcaa gggcggcaag gacgccaacc
      721 aggccgcaag cgcggcatcc gaactgcgtg accgcgccaa aaagcagatg gagagcctgc
      781 gtaaggacgc gctgctctgc acagctgatg agtttgctgc cgaccgccgc cgcgccgcgc
      841 ccctggtggc cgcgctggtg tgggcgacgc agaccttcgc cgacaactgc tttgcggcca
      901 agtgcgctga aaaagtattg gactacgccg attatgagca cctgacgctg gatcttttgc
      961 tgaccgagga caacgagcgc acgccgctct gccgcacggt cagcgcccgc tacgccgccg
     1021 tactggtgga cgagtaccag gacacaaacg ctttgcagga cgccatctac tttgcactgg
     1081 cccggcccga ggccgacaac ctgttctttg tcggcgatat caagcagagc atctaccgct
     1141 tccgtctggc ggatcccagc atttttgtgg gcaaacagca gcagtggcgg ccctatccgc
     1201 agcccatgcc caagcccgcc acgctggcgc tggatgccaa cttccgcagt gcgcccggcg
     1261 tcatcgaggg catcaactac ctgtttgaga ccttcttctc ggaggggctg ggcggcgtgc
     1321 agtacggcga cggccagcgc cttgttgtgg gccgcaagga cagcgactac cgcggtatgt
     1381 gcgagatcga cgtcatcgac ggcgcggatg tggacggcga cgcggcggcc attgcccggc
     1441 gcattgccga aatggtcgag accggctttg ccgtgcgcgg caaggatggc gtgcgcccct
     1501 gcaaatacga tgatttttgt atcctgcttc gcggccgcaa gggctttgcc gcctacgagg
     1561 gcgcactgcg cacggcgggc atcccggtgt ttgcggactc tgccaccgat ttgctggacg
     1621 aaccgcacat ccgccccttc gcggcgctgc tgcgcgttat cgacaaccct gcgcaggata
     1681 tcccgctggc ggcggtgctg c
//
LOCUS       DBMDSQ010000404         1684 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_15480_length_1684_cov_1.077069, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000404 DBMDSQ010000000
VERSION     DBMDSQ010000404.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 1684)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1684)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1684
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15480_length_1684_cov_1.077069"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(108..746)
                     /locus_tag="ACZPAX_10520"
     CDS             complement(108..746)
                     /locus_tag="ACZPAX_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005920655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HIR1114369.1"
                     /translation="MKTHILCLGDSNTHGYCADPKDNADGGIRFNEDERWTCRLQTAL
                     GDKYLVTEEGLSGRTTVFVDPIHESMDALSVAYPLLKSHEVIDLLIIMLGTNDVKERF
                     NANAACIGAGMERLIQKCKTVDCWGGKEPNILVVAPPRIKEGFHDEVMGDGCVEKSRG
                     VSGWYKIIAERNNVHFLDAEGCEFNQIDFMHLTRKGHAQLAEKLAELVPTLL"
     gene            complement(777..1682)
                     /locus_tag="ACZPAX_10525"
     CDS             complement(777..1682)
                     /locus_tag="ACZPAX_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005934432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HIR1114370.1"
                     /translation="MGGTAVKLGIVDETGAVGPKAEESVNFDGYQTPVLATVLKAARA
                     FVETQNIDVNTLEGVGVSATGQIDSRKGIVAGTCGNFPNYINSPIQAELERLFGLPVT
                     VANDANCMTLGEVWVGGAKGYTDVIGVTLGTGVGGGILTGGRLLEGARGLGGELGHYR
                     THALDGVPCTCGATGCWERYAATTALVRAAQEKDPAWKDGRAIFSAAEAGNETVLALL
                     DAWTDEIAQGLAGMVHIFNPQLILIGGGVSAQQKLLIEPIAAKVRASVMPAFAEGLEI
                     RAAQLHNDAGMVGAVYYFRQQHGEA"
BASE COUNT          307 a          510 c          520 g          347 t
ORIGIN      
        1 acaaaagaaa acaaccctct ccgtcaaagc cttttgcttt gcctgcttcc cccgaaaggg
       61 tgaggcggca ttgcaaggca atgacggaga gggttgttct ttttgactca gagcagcgtc
      121 ggcaccagct cggccagctt ttcggccagc tgtgcgtggc ctttacgggt caggtgcatg
      181 aagtcgatct ggttgaactc gcagccttca gcgtccagga aatggacgtt gttgcgctct
      241 gcaatgatct tgtaccagcc ggaaacgccc ctggacttct ccacacagcc gtcgcccatg
      301 acctcatcgt ggaagccctc cttgatgcgc ggaggagcaa ctacaagaat gttgggttcc
      361 ttgccgcccc agcagtccac ggtcttgcac ttctggatca ggcgctccat gccagcgcca
      421 atgcaggcgg cgtttgcgtt gaagcgctct ttcacatcgt tggtgcccag catgatgatg
      481 agcaggtcga tgacttcatg gctcttgagc aggggatagg ccacgctcag agcatccatg
      541 gactcgtgga tggggtctac aaaaacggtg gtgcggccag aaaggccctc ttcggttacc
      601 agatacttgt cacccagagc agtctgcagg cggcaggtcc agcgctcgtc ctcgttgaag
      661 cggatgccgc cgtcggcatt gtccttgggg tcggcacaat aaccgtgggt gttggaatcg
      721 cccaggcaga gaatatgtgt tttcatctca atacactcct ttgatcgttc agatgatcag
      781 gcttcgccgt gctgctggcg gaagtaatag actgcgccta ccataccggc atcattgtgc
      841 agctgggcgg cgcggatctc cagcccctcg gcaaaggcgg gcataacgga ggccctgact
      901 ttggcggcaa tgggctcgat gagcagcttc tgctgggcgc tcacgccgcc gccgatgaga
      961 atgagctgcg ggttgaagat gtgcaccatg cctgcaaggc cctgtgcgat ctcatccgtc
     1021 caggcatcca gcagggccag aacggtctcg ttgccagcct ctgcggcaga aaaaatggcg
     1081 cggccgtctt tccaggccgg gtccttttcc tgtgcggccc gtaccagagc cgtggtggcg
     1141 gcgtagcgct cccagcagcc ggtggcaccg caggtgcagg gaacaccgtc cagtgcatgg
     1201 gtgcggtagt ggcccagctc accgcccagt ccgcgggcac cctccaaaag gcggccgccg
     1261 gtcaggatgc cgccgcccac gccagtgccc agggtcacgc cgatgacatc ggtataaccc
     1321 tttgcgccgc cgacccagac ctcgcccagc gtcatgcagt tggcatcgtt ggcgacggtg
     1381 acgggtaggc cgaagagccg ttccagttcg gcctggatgg ggctgttgat gtagttggga
     1441 aagttgccgc aggtaccggc cacgatgcct ttgcggctgt cgatctggcc ggtggccgaa
     1501 acgcccacgc cttccagtgt gttcacgtcg atgttctggg tctccacaaa ggctctggcg
     1561 gctttgagca cggtggccag caccggggtc tggtagccgt caaaattgac gctctcctcg
     1621 gccttggggc ctaccgcgcc ggtctcatcc acgatgccca gcttgacggc agtgccgccg
     1681 atat
//
LOCUS       DBMDSQ010000405         1683 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_15489_length_1683_cov_1.724399, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000405 DBMDSQ010000000
VERSION     DBMDSQ010000405.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 1683)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1683)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1683
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15489_length_1683_cov_1.724399"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..502)
                     /locus_tag="ACZPAX_10530"
     CDS             complement(<1..502)
                     /locus_tag="ACZPAX_10530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015564986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HIR1114371.1"
                     /translation="MLTNEYLKRVYEGLEKRNANEPEFLQAVREVLESIQPVVEKHPE
                     YEKAGLIERLVEPERIITFRVPWVDDAGKVQVNRGYRVQFNSAIGPYKGGLRFHPSVN
                     QGILKFLGFEQTFKNSLTTLPMGGGKGGSDFDPHGKSDMEVMRFCQSFMTELYRHIGQ
                     FVDCPAG"
     gene            complement(897..>1267)
                     /locus_tag="ACZPAX_10535"
     CDS             complement(897..>1267)
                     /locus_tag="ACZPAX_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005923452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HIR1114372.1"
                     /translation="GLCHDHAYQLLPVDGIVVVASPQELVSMIVAKAVNMAEMMKVPM
                     LGIVENMSYIVCPDCGKHINVFGNSHVDEVAAKHHLPVLAKCPIDPKLAELSDAGMIE
                     TYGGEFLEGAADACEKLLKK"
     assembly_gap    1268..1277
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1278..>1683)
                     /locus_tag="ACZPAX_10540"
     CDS             complement(<1278..>1683)
                     /locus_tag="ACZPAX_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015537868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HIR1114373.1"
                     /translation="PNSSVKKVIGVVSGKGGVGKSMTSALLACAMARRGYHCGILDAD
                     ITGPSIPKLFGIHGRAMADDKGCWPIQSRMGIDVMSINLLVENEEDPVVWRGPVIAGA
                     VKQFWTDVVWKDVDFLFVDMPPGTGDVPLTVFQ"
BASE COUNT          359 a          497 c          463 g          354 t
ORIGIN      
        1 caccggcggg gcagtccacg aactggccga tgtggcggta cagctcggtc atgaagctct
       61 ggcagaagcg catcacttcc atgtcgctct tgccgtgggg gtcaaagtca gagccgccct
      121 tgccgccgcc catgggcagg gtggtcaggc tgttcttgaa ggtctgctcg aagcccagga
      181 acttcaggat gccctgattg acagagggat ggaagcgcag gccgcccttg taggggccga
      241 tggcgctgtt gaactgcacg cggtagccgc ggtttacctg caccttaccg gcatcgtcta
      301 cccaaggcac acggaaggtg atgatgcgct ccggctccac caagcgctcg atcagaccgg
      361 ccttctcgta ctcggggtgc ttttccacaa cgggctggat gctctccagc acctcgcgca
      421 cagcctgcag aaactcgggc tcgttggcgt tgcgcttttc cagaccctcg taaacacgct
      481 tcagatattc attcgtcagc ataatagaat actccttttc ccaattactc tataaatttg
      541 aaaaagcgga aatgcccctg cccgccgcac agcgggtata tccttgcaga tgccgggcgc
      601 ttccggttct ccatgcttgc acggcacgcc cgcaatggcg ggcaatgctt actttattat
      661 tataaagctt tgcggtgggt tttacaatag caaaaagcag aatttgttta aaaaaacaga
      721 taaaaagtca cagctgcttc acaaaacgaa aattaaaagc cggaatgcgc cgcgaacctt
      781 gttttttaaa gtgcgctgcc gccccaaagc tgcacagaaa agaatgtctt ttgcacggca
      841 gcgggcttat ctaagcaaaa agccctgtgt cgcaacgggc acagggcagg gtgcagttac
      901 ttcttcagca gcttttcgca ggcgtctgcg gcgccttcca aaaattcgcc gccatatgtc
      961 tcgatcatgc cggcgtcgga aagctctgcc agtttggggt cgatggggca cttggccagc
     1021 acgggcaggt gatgctttgc ggcaacctcg tccacatggc tgttgccaaa cacattgatg
     1081 tgcttgccgc agtccgggca gacgatgtag ctcatgtttt ctacgatgcc cagcatgggc
     1141 accttcatca tttcggccat gttcacggcc tttgccacga tcatgcttac cagctcctgc
     1201 gggcttgcca ccacaacgat gccatccacc ggcaggagct ggtaagcatg atcgtggcaa
     1261 aggccgtnnn nnnnnnnctg gaacacggtc aggggcacat caccggtgcc cgggggcata
     1321 tccacgaaca gaaaatccac gtccttccag accacatcgg tccagaactg cttgacggca
     1381 ccggcaatga ccgggccgcg ccacacaacg gggtcttcct cgttctccac cagcaggttg
     1441 atgctcatta catcaatgcc catgcggctc tggatgggcc agcagccctt gtcgtccgcc
     1501 atggcacggc catgaatgcc gaacagcttg gggatggacg ggccggtgat atcggcatcc
     1561 agaatgccgc agtggtagcc gcggcgggcc atggcacagg ccagcagggc gctggtcatg
     1621 ctcttgccca cgccgccctt gccggaaaca acgccgatga cctttttcac actgctattg
     1681 ggg
//
LOCUS       DBMDSQ010000406         1678 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_15544_length_1678_cov_1.830057, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000406 DBMDSQ010000000
VERSION     DBMDSQ010000406.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 1678)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1678)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1678
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15544_length_1678_cov_1.830057"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            277..>1678
                     /gene="dnaK"
                     /locus_tag="ACZPAX_10545"
     CDS             277..>1678
                     /gene="dnaK"
                     /locus_tag="ACZPAX_10545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HIR1114374.1"
                     /translation="MAKMIGIDLGTTNSCVAVVEGGKPVVIPNAEGQRTTPSVVAFTK
                     AGERLVGEPAKRQAVTNAPRTVTSVKRLMGSETRVPIDGKCYTPQELSALILQKLKTD
                     AEAYLGESVTEAVITVPAYFNDAQRQATKDAGRIAGLNVRRIVNEPTAAALAYGLDNG
                     RAQTVMVYDLGGGTFDVSLIRIGDGIVQVLATCGDNFLGGDDFDERIMNWLAEQCKTE
                     HGVDLTNDRVAMQRLREEAEKAKKELSSAKQTDINLPFITTTAEGALHLQTTLTRAKF
                     EELCADLIERTALPVRNALSDAHISASDLDQVLLVGGSTRIPAVQAKVMRMTGLEPSK
                     SLNPDECVAMGAALQAGRLGGEMLAGPGEGLLLMDVTPLTLSIETVGGVATHLIERNS
                     TIPTRFSKVFTTAAPFQNTVQIKVLQGEREFAKDNKLLGTFTLRGIKRAWAGVPKIEV
                     TFDLDANGIVKVSARDM"
BASE COUNT          382 a          507 c          494 g          295 t
ORIGIN      
        1 atctgcgttg ccaaaatgct cgataagaca tccagtatta tctgcgcttt tggcttagca
       61 gctgccgcac ctcgctcgcc gtatcggcac ttagaattat gtggtattgc cctaaatata
      121 gcacgccaga caaaaagcgt caacggaaaa ttgcagataa cttactttta taaaaatgac
      181 tgcaatattt acgaaatcgc aacacatttt cctgcggcaa acgttacaat aaaaacaaga
      241 gttaaaattg tcttttttga aaggacaaac accaccatgg caaaaatgat cggaattgat
      301 ctgggcacca ccaacagctg tgtggcggtg gtcgaaggcg gcaagccggt cgtcatcccc
      361 aatgccgagg gccagcgcac aacgccctcg gtcgtggcct tcaccaaagc gggggagcgg
      421 ctggtcggcg agcctgccaa gcggcaggct gtaaccaatg ccccccgcac cgtgaccagc
      481 gtcaagcggc tgatgggcag cgagacccgc gtgcccatcg atggcaagtg ctacacgccg
      541 caggaactga gtgcgcttat cctgcaaaaa ctgaagaccg acgccgaggc ctacctgggc
      601 gagagcgtca ccgaggcggt catcactgtg cccgcctact ttaacgatgc ccagcgccag
      661 gccaccaagg acgccgggcg cattgcgggg ctgaatgtgc gccgcatcgt caacgagcct
      721 acggccgccg cgctggccta cggcctggac aatggccgcg cccagaccgt catggtctat
      781 gacctgggcg gcggcacgtt tgacgtgtcg ctcatccgca ttggcgacgg catcgtgcag
      841 gtgctggcca cctgcggtga caatttcctc ggcggcgacg actttgacga gcgcatcatg
      901 aactggctgg ccgagcagtg caaaaccgag cacggcgttg acctgacgaa tgaccgcgtg
      961 gcgatgcagc gcctgcggga agaagccgaa aaagcaaaaa aagaactttc cagcgcgaaa
     1021 caaacggata tcaacctgcc gtttatcacc acgaccgccg aaggtgcgct gcatttgcag
     1081 acgacactga cccgtgctaa gttcgaggaa ctgtgcgccg atctgatcga gcggacggcc
     1141 ctgccggtgc gcaacgcttt gagcgacgcg cacatttcgg ccagcgacct ggaccaggtc
     1201 ttgctggtgg gcggctcgac ccgcatcccc gccgtgcagg caaaagtcat gcggatgacc
     1261 gggctggaac cgtccaaatc cctgaacccc gacgaatgtg ttgccatggg cgcagccttg
     1321 caggccgggc ggctgggcgg cgaaatgctg gccggccctg gcgagggcct gctgctgatg
     1381 gatgtgactc cgctgaccct ctcgatcgag accgtcggcg gcgtggcgac acatctgatc
     1441 gagcgcaact caacgatccc gacgcggttc agcaaggtat tcaccaccgc cgcgccgttt
     1501 cagaacaccg tgcaaatcaa ggttctgcag ggcgaacgcg agttcgccaa ggacaataag
     1561 ctgctgggca cctttaccct gcggggcatc aagcgtgcct gggcgggcgt gcccaaaatc
     1621 gaggtgacct ttgaccttga cgccaacggc atcgtcaagg tcagcgcccg cgacatgg
//
LOCUS       DBMDSQ010000407         1675 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_15579_length_1675_cov_1.760483, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000407 DBMDSQ010000000
VERSION     DBMDSQ010000407.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 1675)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1675)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1675
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15579_length_1675_cov_1.760483"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1362)
                     /locus_tag="ACZPAX_10550"
     CDS             complement(<1..1362)
                     /locus_tag="ACZPAX_10550"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006574748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HIR1114375.1"
                     /translation="MKFSEMPYARPDLDAVKQQFADLRTRLESAKSYAEAREIFLEEE
                     TLNKHVDTAAQLASVRNSIDTRDKFYDEEMNFWNEAIPQLQECQNAWSQAMLASPFRK
                     EFEAEYGSLMFVNAEIADKAFSPAILDEMAQENKLTTEYGKLIASAQIPFEGGVYTLS
                     QLSPFKNDPDDARRLAAWKAEGAWYKEHGAEFDGLYDQLVHLRDTMGKKLGYEGYTTL
                     GYYRMGRNCYTKADVEKFRAAVVKYIVPLADSIYREQAGRLGKQYPMNAADNALMFRS
                     GNPRPAGDADAILKQGKKFYEELSPETGVFFNKMLDDQLMDVLSTPGKAGGGYCTSLG
                     DYEMPFIFANFNGTQHDVEVVTHEAGHAFAAYMNRDRIPYSYVWPSMEACEVHSMSME
                     FFAWPWADGFFGADARKFRYSHLAGALTFIPYGTMVDHFQHIVYENPELTPAQRHEEW
                     KKLA"
     gene            1541..>1675
                     /locus_tag="ACZPAX_10555"
     CDS             1541..>1675
                     /locus_tag="ACZPAX_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012703744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HIR1114376.1"
                     /translation="MDNIKILEVKQSIFASNDEQAAALRADLKAKKVFLLNLMSAPGS
                     G"
BASE COUNT          295 a          466 c          531 g          383 t
ORIGIN      
        1 agccagcttt ttccattcct cgtggcgctg ggccggggtc agctcggggt tctcgtagac
       61 gatgtgctgg aaatggtcca ccattgtgcc gtaggggatg aaggtcagcg cacccgccag
      121 atggctgtag cggaacttgc gggcatccgc gccgaagaag ccatcggccc acggccaggc
      181 gaagaactcc atcgacatcg agtggacttc gcatgcctcc atgctgggcc agacgtagga
      241 gtacgggatg cggtcacggt tcatgtaggc ggcaaaggcg tggccggcct cgtgggtcac
      301 gacctcgacg tcgtgctggg tgccgttgaa gttggcaaaa ataaacggca tttcgtaatc
      361 acccaggctc gtgcagtagc caccgcccgc cttgccgggg gtggacagca cgtccatgag
      421 ttggtcgtcc agcattttgt tgaagaacac gccggtctcg ggggaaagct cctcatagaa
      481 ctttttgccc tgtttgagga tggcgtcggc gtcgcccgcc gggcgggggt tgccgctgcg
      541 gaacatcagg gcgttgtcgg ctgcgttcat cgggtactgc ttgcccagcc gcccggcctg
      601 ctcgcggtaa atgctgtcgg ccagcggcac gatgtacttg acgacggcgg cgcggaactt
      661 ttccacatcg gccttcgtgt agcagttgcg gcccatgcgg tagtagccca gcgtggtgta
      721 gccctcatag ccgagctttt tgcccatggt gtcacgcagg tggacgagct ggtcgtagag
      781 gccgtcaaac tcggccccgt gctctttgta ccatgcgccc tcggccttcc aggcggccag
      841 gcggcgggca tcgtcggggt cgttcttgaa gggcgagagc tggctcaagg tatagacgcc
      901 gccctcaaag gggatctgcg ccgacgcgat gagtttgccg tactcggtgg tcagcttgtt
      961 ttcctgggcc atctcgtcca ggatggcggg cgagaacgcc ttgtcggcga tctcggcgtt
     1021 gacgaacatc aggctgccgt actcggcctc gaactccttg cggaaaggcg acgccagcat
     1081 agcctggctc catgcgttct ggcactcctg cagctggggg atggcctcat tccagaagtt
     1141 catctcctcg tcgtagaatt tgtcacgggt gtcaatgctg ttgcgcacgc tggccagctg
     1201 ggcggcagtg tcaacgtgtt tgttgagcgt ttcctcttcc aggaagattt cgcgggcctc
     1261 ggcgtaggat ttggcgcttt ccagccgggt gcgcaggtcg gcgaactgct gcttgaccgc
     1321 atccagatcc gggcgggcat agggcatttc tgagaatttc atggggtgtc tcctttgctt
     1381 gtttttgttt gtattatagc acggggaggg tgcgggcgca acgcctcgtt ttttcgtcaa
     1441 atcacccacc ccgccctttc ttttttggcg gattcgtcga ttgcggattt ttatacacaa
     1501 tgttaaaata taaataatac cactttataa ggagcatccc atggacaaca taaaaattct
     1561 ggaagtgaag cagagcatct ttgcttccaa cgacgagcag gccgccgcgc tgcgggctga
     1621 cctgaaagcg aagaaggtgt ttctgctcaa cctgatgagc gcaccggggt ccggc
//
LOCUS       DBMDSQ010000408         1675 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_15582_length_1675_cov_1.705845, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000408 DBMDSQ010000000
VERSION     DBMDSQ010000408.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 1675)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1675)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1675
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15582_length_1675_cov_1.705845"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..473)
                     /locus_tag="ACZPAX_10560"
     CDS             complement(<1..473)
                     /locus_tag="ACZPAX_10560"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015537257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0004473 - malate dehydrogenase
                     (decarboxylating) (NADP+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent malic enzyme"
                     /protein_id="HIR1114377.1"
                     /translation="MDYNKAALELHSKFPGKIAVQSLCECKDRDSLSTAYTPGVAEPC
                     RKIKANPDDVYTYTMKGRTVAVVTNGTAVLGLGNIGPEAGLPVMEGKCVLFKEFGGVD
                     AFPICLNAKTKEEVIAAVKAIAPTFGGINLEDIRSPECFEIEAELERELDIPVFH"
     gene            complement(610..>1675)
                     /locus_tag="ACZPAX_10565"
     CDS             complement(610..>1675)
                     /locus_tag="ACZPAX_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HIR1114378.1"
                     /translation="GVLTGSVAITADGMNNLSDASSNIVSLVGFKLGARPADSEHPYG
                     HARYEYLAGLAVSVMILVIGVELLKESFAKVLHPTAVAFNWVMVAVLAASILVKLWMS
                     ALNKHIGTAIESETLLATAADARNDVITTAAVLAATILTKLTGFDRIDGLMGLGVAAF
                     ILYSGAMLVKDTIDPLLGAAPDPELVEHIEKKALSYPGVLGVHDLMIHDYGPGSRLVS
                     FHLEMDAKDDVMHSHDVIDRVERDLLVEDGVIATVHFDPIVTGDAHVDELKTFLQNEV
                     KELEPLANIHDLRIVPGPTHTNVIFDCAVPAEYMADKQHRGVKLVNALRNSVQAKWPD
                     HFCVIKLEPDYAPVHHEGKT"
BASE COUNT          318 a          470 c          504 g          383 t
ORIGIN      
        1 tcatggaaca ctgggatatc gagttcacgc tcgagttcgg cctcaatttc aaagcactcg
       61 gggctgcgga tatcctcaag gttgatgccg ccgaacgtcg gggcgattgc cttgacggca
      121 gcgataactt cctctttggt tttggcattc aggcagatgg gaaatgcatc gacgccgccg
      181 aactccttga agaggacgca ttttccctcc atgacgggca ggccggcctc ggggccgatg
      241 ttgcccaagc ccagcactgc cgtaccgttg gtgacgaccg caacggtgcg gcccttcatc
      301 gtgtaggtgt agacgtcgtc gggatttgct ttgattttac ggcagggttc ggccacgccg
      361 ggggtgtagg cagtagagag tgagtcacgg tccttgcatt cgcaaaggct ctgcacggcg
      421 attttaccgg ggaatttgct gtgaagttcc agggctgctt tgttgtaatc catgctgtat
      481 gtctccttta tatggtttta ctttccgcaa ggccgctcga tagaggtcgg ctatgtccgg
      541 cccgctgctt tgcctttaca tcgctattat ttttacgccg agggcagggc ataccctgcc
      601 cctacgtggt tacgtttttc cctcatgatg caccggagcg taatcaggtt ccaactttat
      661 gacgcaaaaa tggtcgggcc atttagcctg tacggaattg cgcaaggcgt tgacgagctt
      721 gacgccgcgg tgctgcttgt cggccatgta ctcagccggc acggcacagt caaaaatcac
      781 gttggtatgc gtcgggccag gcacgatgcg caaatcgtgg atgtttgcca gcggttccag
      841 ctccttgacc tcgttctgta aaaaggtttt cagctcgtcc acgtgggcat caccggtgac
      901 gatggggtca aagtgtaccg tggcgatcac cccatcctcg acgagcaggt cacgctcgac
      961 acggtcaatg acatcgtggc tgtgcatgac gtcgtccttg gcgtccattt ccaggtggaa
     1021 gctgacaagg cggctgccgg ggccgtaatc gtggatcatc aagtcatgca cgcccagcac
     1081 gccggggtag ctcaatgcct ttttctcgat gtgctccacc aattccgggt cgggggctgc
     1141 gcccagcagc gggtcaatgg tgtctttcac aagcatggca ccgctgtaca ggatgaacgc
     1201 cgctacgccc aggcccatca ggccgtcgat gcggtcaaag ccggtgagct tcgtcaaaat
     1261 cgtagctgcc agcaccgctg ccgtggtgat gacgtcattg cgggcgtcag cggcagtggc
     1321 aagcagcgtc tccgactcaa tggctgtgcc tatgtgcttg ttgagggcac tcatccagag
     1381 cttgacgagg atggacgctg ccagcaccgc gaccatgacc cagttgaagg ccaccgccgt
     1441 ggggtgcagg actttggcga agctctcttt cagcagttcc acgccgatga cgagaatcat
     1501 caccgacaca gccagcccgg ctaggtattc ataccgggcg tggccatagg gatgttccga
     1561 gtctgccggg cgtgcgccga gcttgaaccc cacaaggctt acgatattgg aactggcgtc
     1621 ggagaggttg ttcatgccgt ctgcagtgat ggcaacgctg ccggtcagca cgccg
//
LOCUS       DBMDSQ010000409         1674 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_15597_length_1674_cov_1.779402, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000409 DBMDSQ010000000
VERSION     DBMDSQ010000409.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 1674)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1674)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1674
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15597_length_1674_cov_1.779402"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            23..1291
                     /locus_tag="ACZPAX_10570"
     CDS             23..1291
                     /locus_tag="ACZPAX_10570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015538425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016852 - sirohydrochlorin
                     cobaltochelatase activity [Evidence IEA];
                     GO_process: GO:0019251 - anaerobic cobalamin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sirohydrochlorin cobaltochelatase"
                     /protein_id="HIR1114379.1"
                     /translation="MRKRNHKKPAAVLAALALTTALLAGCGSTAATTESTAESTAESA
                     AASESEAVSSEETADEGDADEVAAKNCADLIDAIYVQERTDDTDAQCEAAKAAWDALT
                     DTQKEMVEGENADPEYFGRDTGDAGNDDPRNEDEIGENELLVVSFGTSFNSSRAADIK
                     GIEDALQAANPDWSVRRAFTAQIIINHVQARDGEKIDNMGQALDRAVANGVKNLVVQP
                     THLMHGTEYDEMCEAVEQYKDKFESVAIAEPLLGEVGEDATVINADKEAVAAAITAEA
                     VKVAGYDDAAAAAADGTAFVFMGHGTSHTAKISYSQMQTTMQTLGYDNVFIGTVEGEP
                     EDTACDAVIEKVKEAGYTKVILRPLMVVAGDHANNDMAGSDEDSWLSQFNAAGCFDSV
                     DTQIAGLGEIADVQQLYVDHTKAAIDSLNG"
BASE COUNT          361 a          541 c          504 g          268 t
ORIGIN      
        1 caacaggaaa ggaagcaaac acatgagaaa acgcaaccac aaaaagcctg ctgccgtgct
       61 ggcagccctg gccctgacca ccgcgctgct ggctggctgc ggcagcaccg ccgctaccac
      121 cgagagcacc gctgagagca ccgctgagag cgccgctgcc agcgagagcg aagccgtaag
      181 cagcgaggaa accgcagatg agggagatgc cgacgaagtg gccgcgaaaa actgcgctga
      241 tttgatcgac gccatctatg tgcaggagcg caccgacgac accgacgctc agtgcgaggc
      301 cgccaaggct gcctgggacg ccctgaccga cacccagaag gaaatggtcg agggcgagaa
      361 cgccgacccc gagtatttcg gccgtgacac cggtgacgcc ggcaacgacg acccccgcaa
      421 cgaggatgag atcggcgaga acgagctgct ggtcgtcagc ttcggaacct ccttcaacag
      481 cagccgcgcc gctgacatca agggcatcga ggatgcctta caggccgcta accccgattg
      541 gagcgtgcgc cgcgccttca ccgcgcagat catcattaac catgtgcagg cccgcgacgg
      601 cgagaaaatc gacaacatgg gccaggcttt ggatcgcgcc gttgccaacg gcgttaagaa
      661 cctggtcgtc cagcccaccc atctgatgca cggcaccgag tacgacgaga tgtgcgaggc
      721 cgttgagcag tacaaggaca agttcgagag cgttgccatc gccgagccgc tgctgggcga
      781 ggttggcgag gatgccaccg tcatcaacgc cgacaaggag gctgttgccg ctgccatcac
      841 cgccgaggcc gtcaaggtcg ccgggtatga cgatgccgct gctgccgctg ctgacggcac
      901 cgcctttgtg ttcatgggcc acggcacgtc ccacaccgcc aagatcagtt acagccagat
      961 gcagaccacc atgcagaccc tgggctatga caatgtgttc atcggcactg tcgagggcga
     1021 gccggaggac accgcctgcg acgctgtcat cgagaaggtc aaggaggccg gttacaccaa
     1081 ggtcatcctg cgcccgctga tggtcgttgc cggtgaccac gccaacaatg acatggccgg
     1141 ctctgacgag gattcctggc tgagccagtt caacgccgct ggctgctttg acagtgtcga
     1201 cacccagatc gccggtctgg gcgagatcgc cgatgtccag cagctctacg ttgatcacac
     1261 caaggcagct atcgattccc tgaacggtta agtttgatac attctgcccg aagggaacgc
     1321 cttcgggcag tttttggttt tgggggatag aaagccattt tcggtggaga ggggagggat
     1381 tcatcccgcc cgcgccgtat gccgttattg ctcgatttcc ccgtgtgccg cccatgcgcg
     1441 gcccctgccg tgcggtgttc ccttaattta tatatgttag aatagagaca ggaaggtaac
     1501 catgaaaaaa cgccaactcg catccatcac actggccgcc ctgctgctgg cgggctgcgg
     1561 cgcatccgca tccaccaccg ccgagagtgc atccgcggct gcctccaccg tggagaccgc
     1621 ccccgaagcc accgaggccc ccgccgcaac cgaagcccct gccgccgccc tgcc
//
LOCUS       DBMDSQ010000410         1671 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_15630_length_1671_cov_1.611465, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000410 DBMDSQ010000000
VERSION     DBMDSQ010000410.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 1671)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1671)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1671
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15630_length_1671_cov_1.611465"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="ACZPAX_10575"
     CDS             complement(<1..287)
                     /locus_tag="ACZPAX_10575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015537620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AD"
                     /protein_id="HIR1114380.1"
                     /translation="MATRRGDTLIFPKPPVIAAHACIGGKKEGESPLAAEFDELHSDN
                     RLGQASWEAAETQLQLQTARLCLKKAHATEKDVSLLLAGDLQAQCTASGYAA"
     gene            complement(423..863)
                     /locus_tag="ACZPAX_10580"
     CDS             complement(423..863)
                     /locus_tag="ACZPAX_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016320405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVA/SpoVAEb family sporulation membrane
                     protein"
                     /protein_id="HIR1114381.1"
                     /translation="MKMTAEEYARRVKQVGPRSPLGSDCLWAFCVGGGICLLGEVLRG
                     WYLGMGLEAQLAGTLTSCTLIVLSALLTTLGLYQKLATKAGAGSLVPITGFANAVVSA
                     AIEFKPEGRVCGTGAKMFTIAGPVIVYGTLAAVVYGGVLWLLGG"
     gene            940..>1671
                     /locus_tag="ACZPAX_10585"
     CDS             940..>1671
                     /locus_tag="ACZPAX_10585"
                     /EC_number="3.4.17.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005943565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HIR1114382.1"
                     /translation="MNQQQPIHRRRLTRAEIRRRRRNRAIRRIAGLTLVVCVAVGGVS
                     FLLSRRKAPVPTADAAGSAANSAASSLPSAAPSSKAESSAVSGAAAESVESRDNLLGL
                     TAAEAKAMLADPLMILVNHTNKMPENYTFETAECGSKTAVNKTLQTVACNAFLELQKA
                     AAAENVTVWMQSGYRSVSYQTNLYEKKTNYYKQQGYDDAKAKEMAAAIVNPPGYSEHN
                     CGLAADLNSPEHTGLDEGFENTAAFR"
BASE COUNT          324 a          544 c          516 g          287 t
ORIGIN      
        1 gccgcatagc ccgaagccgt gcactgggcc tgcaggtctc cggccagcag gaggctcaca
       61 tccttttcgg tggcgtgggc ttttttcagg cagagccgcg ccgtctgtaa ttgcagctgc
      121 gtttcagcag cctcccagct ggcctgcccg aggcggttgt cgctgtgcag ctcatcgaac
      181 tctgccgcca gcgggctttc gccctccttt ttgccgccga tgcaggcgtg tgcggcgatg
      241 acaggcggtt ttggaaagat cagggtgtca ccccggcggg tcgccatgaa aaaatccccc
      301 tttcagggct gtttttcttt gggatttgtg ctataatagc gctcgtgcga tattctgctc
      361 cagccgctct gccggaaaac tgcaattttt gcatcccggc aggcgtattg tagacagagc
      421 ggtcagccgc ccagcagcca gagcacccca ccgtagacca cagcggccag cgtgccgtac
      481 acgatgactg gccctgcaat ggtgaacatc ttggctccgg tgccgcagac cctgccctcg
      541 ggcttgaact cgatggcggc gctgaccacg gcgttggcaa agcccgtgat gggcaccagc
      601 gagcctgccc cggctttggt ggcaagcttc tggtagagcc ccagcgtggt cagcagggcc
      661 gacagcacga tgagggtgca gctggtcagg gtaccggcaa gctgggcttc cagccccatg
      721 ccaagatacc acccgcgcag cacctcgccc agcaggcaga tgccgccgcc cacgcagaag
      781 gcccacaggc agtcggagcc cagaggggag cgggggccga cctgtttcac ccgccgcgcg
      841 tattcttccg ctgtcatctt catggctgtc acctcgggct taggatgccc cccggagaaa
      901 cataatatac agaaaacgaa ccgagaaaga aggtctctta tgaatcagca gcaacccatc
      961 caccgccgcc gcctgacccg ggcagagatc cggcggcggc gacgcaaccg cgccatccgc
     1021 cgcatcgccg ggctgaccct tgtggtctgc gtggccgtgg gcggtgtgtc cttcctgctt
     1081 tcccgccgca aggccccggt gcccacggcg gatgccgccg ggagtgccgc caatagtgcg
     1141 gccagcagcc tgccgtcggc tgcgccgagc agcaaggcgg agagcagtgc cgtgagcgga
     1201 gccgccgccg agagcgtgga gagccgggac aacctgctgg gcctgactgc cgccgaggcg
     1261 aaggccatgc tggccgaccc gctgatgatc cttgtgaacc acaccaataa gatgccggaa
     1321 aactacacct ttgagaccgc cgagtgcggc tccaagaccg cagtgaacaa gaccctgcag
     1381 accgtggcct gcaatgcctt tctggagctg cagaaggctg ctgctgcgga gaacgtgacc
     1441 gtctggatgc agagcggcta ccgcagtgtg tcctaccaga ccaatctcta cgagaaaaag
     1501 accaactatt ataagcagca gggctatgac gatgccaagg caaaggaaat ggctgctgcc
     1561 atcgtcaacc cgccaggcta ttctgagcac aactgcggcc tggctgcaga cctgaacagc
     1621 cccgagcaca ccggcctgga cgagggcttt gagaacacgg ctgccttccg c
//
LOCUS       DBMDSQ010000411         1643 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_16018_length_1643_cov_1.522698, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000411 DBMDSQ010000000
VERSION     DBMDSQ010000411.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 1643)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1643)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1643
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16018_length_1643_cov_1.522698"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..689)
                     /locus_tag="ACZPAX_10590"
     CDS             complement(<1..689)
                     /locus_tag="ACZPAX_10590"
                     /inference="COORDINATES: protein motif:HMM:NF015431.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR1114383.1"
                     /translation="MDTERWQILLRAIDRGSLRAAADEMDVTVSGISRSVATLEKELG
                     FSLLYRAKSGVQPTEECRQLLPTVRDLLFAQERLEQTAAKVRGADCGTIVIGTAYNCY
                     YEWVTRMTAAFRTRHPGVAVRIVNGTSSELAEMVKDHRTDFCLISRRDGLPVWLPIRQ
                     DELLVLVPKSNPLAGMDAVPVQRMKTEPFIESYPGMETDITLLFDKLKTKPHTSYSTM
                     AITACTKRAGCT"
     assembly_gap    862..871
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<872..>1643)
                     /locus_tag="ACZPAX_10595"
     CDS             complement(<872..>1643)
                     /locus_tag="ACZPAX_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HIR1114384.1"
                     /translation="DTFEVNLRPRIFRKLQHHIAKVTGLSQGDLDAGLPMKEGLQKFL
                     DWAGPDAELAEWGLDDVPVLKQNLFLVGLDENWPDKWYDLQRIFLQAYPRKEGEGLTL
                     ESVVDRLGIPKEEPFHNALDDALYTAKICRKLPLAEGLATYPTEEELLTEALLGAEKT
                     GRDVKLFMNRMEHDDYRNEPELNQAACPECGAPLQNDEVWLKRGNTGYFTRATCPCCG
                     HYYLRFKLSRRDGLHWSFARCIDPATPESDARWDKQKAA"
BASE COUNT          289 a          494 c          482 g          368 t
ORIGIN      
        1 gtgcatcccg cccgtttagt gcaagccgtg atggccatgg tggaatagct cgtgtgcggc
       61 ttggtcttga gcttatcgaa cagcagcgtg atatccgtct ccatgccggg gtagctttcg
      121 atgaagggct cggttttcat gcgctgcacc gggacggcgt ccataccggc cagcgggttg
      181 cttttgggca ccagtaccag cagctcatcc tggcggatgg gcagccagac cggcaggccg
      241 tcgcggcggc tgataaggca gaaatccgtg cggtggtcct tgaccatttc ggccagctcg
      301 ctgctggtac cgttgacgat gcgcactgcc acgccggggt gccgggtgcg gaacgccgcc
      361 gtcatccggg tgacccactc ataatagcag ttatacgccg tgccgatgac gatggtgccg
      421 cagtctgcgc cgcgcacctt ggcggctgtt tgctccaaac gctcctgcgc aaagagcaga
      481 tcgcgcacag ttggcaacag ctggcggcat tcctcggtgg gctgcacacc gctttttgcg
      541 cggtacagca gcgaaaatcc aagttctttt tccagcgttg ccacgctgcg gctgatgcct
      601 gaaacagtaa cgtccatctc atccgccgcc gcgcgcaggc tgccccggtc aatggccctc
      661 aacaaaatct gccagcgttc tgtatccatg ctctacctct catgttgcta tatttgcaat
      721 tttgtaaaaa atgtgtttcg tttttatcaa gaatagacca gaatcacagt tttgtcaagg
      781 gcgtacaaca aaaaagcttt ctcccaaggc gcaggcagcc cttttatctt tccgcctttt
      841 tcttcattcg ttccagcagg gnnnnnnnnn ngggcggctt tctgcttgtc ccagcgggcg
      901 tcggattcgg gggtggcggg gtcgatgcag cgggcaaagc tccaatgcag accatcgcgg
      961 cggctgagct tgaaccgcag gtaatagtgc ccgcagcatg ggcaggtcgc ccgggtgaaa
     1021 tacccggtgt tgccgcgttt gagccagacc tcgtcatttt gcagcggcgc accgcattcg
     1081 gggcaggcgg cctggttcag ttccggctca ttacggtagt cgtcgtgctc catccggttc
     1141 atgaagagct ttacgtcgcg gccggtcttt tctgcgccca gcagggcttc ggtcagcagt
     1201 tcttcctcgg tcgggtaggt ggcaaggccc tcggccagcg gcagtttgcg gcagatcttg
     1261 gccgtgtaaa gcgcatcgtc cagcgcattg tggaatggct cctccttcgg gatgcccagg
     1321 cgatccacaa cactttccag cgtcaggccc tcgccctcct tgcgggggta ggcctgcaag
     1381 aaaatacgct gcagatcgta ccatttgtcg ggccagttct cgtccagccc caccaaaaac
     1441 aggttttgtt tcagcaccgg aacgtcgtcc agtccccatt cggccagctc ggcgtccggc
     1501 ccggcccagt ccaggaactt ctgcagcccc tctttcatcg gcaggcccgc gtccaaatcc
     1561 ccctgggaca ggccggtgac cttggcgata tggtgctgca atttgcggaa gatgcgcggg
     1621 cgcaggttga cttcaaacgt atc
//
LOCUS       DBMDSQ010000412         1636 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_16121_length_1636_cov_1.768730, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000412 DBMDSQ010000000
VERSION     DBMDSQ010000412.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1636)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16121_length_1636_cov_1.768730"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(42..830)
                     /locus_tag="ACZPAX_10600"
     CDS             complement(42..830)
                     /locus_tag="ACZPAX_10600"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005934360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HIR1114385.1"
                     /translation="MWEDAAPMQEREPPRPPARPRSEEDLVLWAQILLCLAAVGFVLA
                     AKLLGWPAYPVLRRAFAAATQPEQSLLLGEERGLLKFTEQTAEELANSARSMWDDFTA
                     APETARTPHGQPTPPAYASEDSYTPDFALCFPLPGHFARKTSGYGWRTDPMGGLGDDF
                     HIGNDLAAARGTAVLAAADGVVRTAGSHKSYGNYVRILHADGDETLYAHMQYLFVRAG
                     ERVTAGQTLGTVGQTGNATGPHLHFEILHKGLRYDPAQALQDAA"
     gene            complement(1038..1418)
                     /locus_tag="ACZPAX_10605"
     CDS             complement(1038..1418)
                     /locus_tag="ACZPAX_10605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1114386.1"
                     /translation="MKSCVVTANYACDPNKVWLYLTKPTLNNWRKDVSGYEESDDGMK
                     ATEKNTDGGETQIVFTRKDKPRCLSCSFQKGKISGTFTALLLGGGDSTSVECTLDVNG
                     LGLFTKPAKLMNERLEMLRQTLGA"
     gene            complement(1433..>1636)
                     /locus_tag="ACZPAX_10610"
     CDS             complement(1433..>1636)
                     /locus_tag="ACZPAX_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1114387.1"
                     /translation="QPPVDSQMAMFGYFQYGFEMAPEKPKAKPKPKTAAAKATAKPVA
                     RQHAALRPKRASLLQNLFGRGKK"
BASE COUNT          256 a          509 c          527 g          344 t
ORIGIN      
        1 caaacagcac cgccagcggc ctgcgccagg tgattttggg cctacgctgc atcctgtaaa
       61 gcctgggccg ggtcgtaccg caggcccttg tgcagtatct caaaatgcag gtgcgggccg
      121 gtggcgttgc cggtctgccc caccgtgccc agcgtctgcc cggcggtcac gcgctccccc
      181 gcgcggacaa acaggtattg catatgtgcg tacagcgtct cgtcgccgtc ggcgtgcagg
      241 atgcggacat agttgccata gcttttatgg ctgcccgccg tgcgcaccac gccgtctgcg
      301 gcggccagta ccgccgtgcc ccgggccgct gccaggtcat tgccaatgtg gaaatcgtcg
      361 cccaggccgc ccatggggtc ggtgcgccag ccatagccgg aggttttgcg ggcaaagtgc
      421 cccggcagcg ggaagcagag cgcaaagtcc ggggtgtagc tgtcctcgct ggcgtaggcg
      481 ggcggtgtcg gctggccgtg gggcgtgcgg gctgtctcgg gcgcggcggt gaagtcgtcc
      541 cacatactgc gggcggagtt tgccagttcc tcggcagtct gctcagtgaa tttcaaaagg
      601 ccgcgttcct cccccagcag cagcgattgt tctggctgtg tggcggcggc aaaggcacgg
      661 cgcagcaccg gatacgcggg ccagcccagc agtttggccg ccagcacaaa gccgactgcg
      721 gccaggcaca gcaaaatctg cgcccacagg accaaatctt cctcactgcg cggacgtgcg
      781 ggcggtctgg gcggctcgcg ctcctgcatc ggggctgcat cttcccacat ggcgtacacc
      841 tcccctgttt tttacaacag tatgcgggag gggaaaaaaa tatccgcccc gaaaggcgga
      901 tggaagattg gttataacgg cacacggcca cggcttttcc gcaggaggtc gttttcctta
      961 caggtcaggc ttcggcaagc ccgcctctta tccggcctcg cacactcggc caccttcccc
     1021 catcggggga aggctcttta cgcgcccagc gtctggcgca gcatttccag gcgctcgttc
     1081 atcagcttgg cgggcttggt gaacaggccc aggccgttca catccagcgt acactccaca
     1141 ctggtggagt cgccgccgcc cagcagcagc gccgtaaagg taccgctgat cttgcccttc
     1201 tggaagctgc agctcaggca gcggggctta tccttgcggg taaagacgat ttgggtctcg
     1261 ccgccgtcgg tgttcttctc ggtggctttc atgccatcgt cgctttcctc gtacccgctg
     1321 acgtccttgc gccagttgtt cagggtgggc ttggtcaggt agagccagac cttgttgggg
     1381 tcacaggcgt aatttgcggt cactacacag cttttcatgg tttgggttct ccttattttt
     1441 tcccgcggcc aaacaggttt tgcaaaaggc tggcgcgttt ggggcgcagc gctgcgtgct
     1501 ggcgggccac aggttttgca gtggccttgg cggccgctgt tttgggcttt ggcttggctt
     1561 tgggcttctc gggggccatc tcaaagccgt actggaaata gccgaacatt gccatctggc
     1621 tgtccaccgg gggctg
//
LOCUS       DBMDSQ010000413         1620 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_16321_length_1620_cov_1.023700, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000413 DBMDSQ010000000
VERSION     DBMDSQ010000413.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 1620)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1620)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1620
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16321_length_1620_cov_1.023700"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(82..1380)
                     /locus_tag="ACZPAX_10615"
     CDS             complement(82..1380)
                     /locus_tag="ACZPAX_10615"
                     /inference="COORDINATES: protein motif:HMM:NF024743.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HIR1114388.1"
                     /translation="MNKQTYLAKLAQLLAPLPEQERQDALNYCEEYFDAAGPEAEDAT
                     AAELGDPADAARKILEGEGLMLDSAQAAAGESSDAADTAQDAAPQPTGENPSTTKPKL
                     HGPAAIPPVMGVLLALAAVLVVVCVLLIGFFGVRRSSIANTAETFVVSAAESVVEQPQ
                     DASSTTATPDSEIVEENGTAAEGPSGAVDDNSPHTTDMSCDNITLQDAVLDIDYGRLT
                     IVIDPGATSAALHCENVYNKRLDVTYTENEPLTIRYKVPANCDLSSEPEPEFVLSVPD
                     TETFHFKRLSITSAMSDVEFDNNETITADSIELNLAMGDFTCSTVEAENFNATVSMGN
                     FTGGLVQSSSFTAEVAMGNFDLGRLESSKICTVKAGMGNIGLTVDGKADDYTLDLQAP
                     MGEVAFNGKALGTSYSQTPTTARRVTLTAGMGDVVLTTTE"
     gene            complement(1377..>1620)
                     /locus_tag="ACZPAX_10620"
     CDS             complement(1377..>1620)
                     /locus_tag="ACZPAX_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009260358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HIR1114389.1"
                     /translation="GYRITQDVRAVLDISESTLYPVLRRLQKDGCLTVHDEPFNGRNR
                     RYYQITPTGRAQLAGYRAEWDAYTAKIDRLFEGETQ"
BASE COUNT          290 a          448 c          532 g          350 t
ORIGIN      
        1 gcaccaaaga ttttaaggga agtatgactt tgacatgcga taatggaaat cgcgcggaag
       61 atgcaggggc atggaacggc tttattccgt cgtcgtcagc accacatctc ccatcccggc
      121 ggtcagggtc acgcggcggg cggtggtagg cgtttggctg tagctggtgc ccagcgcctt
      181 gccgttaaag gcgacctcgc ccatcggggc ctgcaggtcc agcgtgtagt catcggcctt
      241 gccgtccacc gtcaggccaa tgttgcccat cccggccttg acggtgcaga ttttggaact
      301 ctccagccgt cccaggtcaa agttgcccat ggcaacctcg gcggtaaagc tgctgctctg
      361 cacaaggccg ccggtaaagt tgcccatcga gactgtggcg ttgaaatttt ccgcttccac
      421 cgtgctgcag gtaaagtcgc ccatggccag gttcagttcg atgctgtcgg cggtgatggt
      481 ctcattgttg tcaaactcca catcgctcat ggcggaagtg atggagagcc ttttaaagtg
      541 gaaggtctct gtatccggca cggacaaaac aaactccggt tccggctcgc tgctcaaatc
      601 gcagttggca ggcactttgt agcggatggt cagcggttca ttctcagtgt aggtaacgtc
      661 gagccgcttg ttatacacgt tctcgcagtg cagggcggcg ctggtggcgc cggggtcaat
      721 gacgatggtc aggcggccgt aatcaatgtc cagcacggca tcctgcaggg tgatgttgtc
      781 acaggacatg tctgtcgtgt gggggctgtt gtcatccaca gcaccggagg ggccttcggc
      841 ggcagtaccg ttttcctcaa cgatttcact gtcgggggtc gcggtggtag aggaagcatc
      901 ctggggttgc tcgacgaccg actcggcagc cgagacgacg aaagtttcgg cggtgttggc
      961 aatagaactg cggcgcacgc cgaagaagcc gatcagtaag acgcagacca cgaccagcac
     1021 ggcggcaaga gccaacaaca cgcccataac cggcgggatg gcggccggac cgtggagctt
     1081 aggcttggta gtggagggat tttctccagt tggttggggg gcagcatcct gcgcggtgtc
     1141 ggcagcgtcg ctgctctccc ctgctgcggc ttgggcggag tccagcatca ggccctcgcc
     1201 ctccaaaatt ttgcgggcgg cgtcggcggg gtcgcccagc tcggcggcgg tggcgtcttc
     1261 agcttccggg cctgcggcgt caaagtattc ttcgcagtag ttcagcgcat cctggcgctc
     1321 ctgctcgggc agaggggcga gcagctgcgc cagcttggct aaataagttt gtttgttcat
     1381 tgtgtctccc cctcaaatag tcggtcgatc ttggctgtgt aggcgtccca ttcggcgcga
     1441 taccccgcca gctgggcacg gccggtgggt gtgatttggt agtagcggcg gttgcggccg
     1501 ttgaacggct catcgtggac ggtcagacag ccgtcctttt gcaggcggcg caaaacggga
     1561 tacagcgtgg attcggatat gtccagtacg gcgcgcacat cctgggtgat gcggtagccg
//
LOCUS       DBMDSQ010000414         1615 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_16375_length_1615_cov_1.654557, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000414 DBMDSQ010000000
VERSION     DBMDSQ010000414.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 1615)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1615)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1615
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16375_length_1615_cov_1.654557"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            259..>1615
                     /locus_tag="ACZPAX_10625"
     CDS             259..>1615
                     /locus_tag="ACZPAX_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002581359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1114390.1"
                     /translation="MAQNEYAVRLEHVTKTFGPVVANKDVSLGVRRGEILALLGENGS
                     GKTTLMNMIAGIYFPDEGEIYVGDKAVTIRSPRDALDLGIGMIHQHFKLVDVFSATEN
                     IALSMGGGEKFDLKKVHDKARAICEKYEFNLDLDQKVYEMSVSQKQTLEIVKVLYRGA
                     DILILDEPTAVLTPQETEKLFSVIRNMKADGKAVIIITHKLHEVLSISDRVAILRKGT
                     YVGDIATRDADEAKLTEMMVGEKVELNIDRPEPVDPVKRLDIQHLTVHSAEGITVLDD
                     ASFPVYGGEILGIAGISGNGQKELLEAIAGLQPTESGASIEYYAPEASAPVQLIGKSP
                     KAIREAGIHLSFVPEDRLGMGLVGSMGMTDNMMLKSYGKGHSPIVDRKAPHDLAETIK
                     KELGVVTPDLNTPVSRLSGGNVQKVLVGRELADYEIINEPDKVQASLKAVKLAHDGVA
                     NM"
BASE COUNT          349 a          495 c          491 g          280 t
ORIGIN      
        1 gacaagggag gctttaaaaa ggtttccttg tcaaaagggg ctgcccgcgt taagcgggct
       61 ggggggattt ttccccgagc cgttggccgc accacaatca caaaattgtt cctgccttga
      121 ttttttgaat ccctgtaggg gcgaggcatg cctcgcccgc agcgtgaccg cgattgcccg
      181 cttatgggtg gccgcgcaag gctgcgggcc gggcatgccc ggcccctacc tacttgactc
      241 ttgaaaggag cctgcataat ggcgcagaac gaatatgctg tgcgcttgga gcacgttacc
      301 aaaaccttcg gcccggtcgt ggccaacaag gatgtctccc tgggcgtgcg ccgcggtgaa
      361 attctggccc tgctgggtga aaacggcagc ggcaagacca cgctgatgaa tatgatcgcc
      421 ggtatctact tccccgatga gggcgaaatc tacgtcggag acaaggccgt gaccatccgc
      481 tccccgcgtg acgcgctgga tctgggcatc ggtatgatcc accagcactt taagctcgtg
      541 gatgtgttct ccgccaccga aaatatcgcc ctgtccatgg gcggcggcga aaaattcgac
      601 ctgaaaaaag tccacgacaa ggcccgcgcc atctgcgaaa aatatgagtt caacctcgac
      661 cttgaccaga aggtctacga gatgagcgtc agccagaagc agacgctgga gatcgtcaag
      721 gtgctctacc gcggcgcgga tatcctgatt ctggacgaac ccacggcggt tctgaccccg
      781 caggagaccg agaagctgtt cagcgtcatc cgcaacatga aggccgacgg caaggcggtt
      841 atcatcatca cccacaagct gcacgaggtg ctgtccatca gtgaccgcgt tgccatcctg
      901 cgcaagggta cgtatgtggg cgacattgcc accagggacg ccgacgaggc caagctgacc
      961 gaaatgatgg tgggcgagaa ggtcgagctg aacatcgacc gccccgagcc ggtcgacccg
     1021 gtcaagcggc tggacatcca gcatttgacc gtgcacagcg ccgagggtat caccgtgctg
     1081 gacgacgcct ccttcccggt ctacggcggt gagattctgg gcattgcggg catttccggc
     1141 aacggccaga aggagctgct ggaagccatc gcgggcctgc agcccaccga gagcggcgcg
     1201 agcattgagt attacgcccc cgaggccagc gccccggtgc agctcatcgg caaaagcccc
     1261 aaggccatcc gcgaggcggg catccacctc tccttcgtgc cggaggaccg cctgggcatg
     1321 ggcctggtcg gctcgatggg catgaccgac aacatgatgc tgaaaagcta cggcaaggga
     1381 cattcgccca ttgtcgaccg caaggccccg cacgacctgg ccgagaccat caaaaaagaa
     1441 ctgggcgttg tcacgcccga tttgaacacc cccgtctccc gcctgtccgg cggcaacgtg
     1501 cagaaggtgc tggtgggccg cgaactggcc gactacgaaa tcatcaacga gcccgacaag
     1561 gtccaggctt cgctgaaggc cgtcaagctg gcccacgacg gtgtggccaa catgt
//
LOCUS       DBMDSQ010000415         1602 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_16566_length_1602_cov_1.139907, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000415 DBMDSQ010000000
VERSION     DBMDSQ010000415.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 1602)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1602)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1602
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16566_length_1602_cov_1.139907"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..233
                     /locus_tag="ACZPAX_10630"
     CDS             <1..233
                     /locus_tag="ACZPAX_10630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HIR1114391.1"
                     /translation="DEEFLDKHPNSHIGAEVEVSELMGAEIYLYLTYQGQNLMARVAP
                     TSKSRRGDKIVVAMDTNKIHLFDPETELTLLN"
     gene            319..>1602
                     /locus_tag="ACZPAX_10635"
     CDS             319..>1602
                     /locus_tag="ACZPAX_10635"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: protein motif:HMM:NF012579.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIB"
                     /protein_id="HIR1114392.1"
                     /translation="MHITDHLQPCCVALRQQADSLPAAIHQLVELVQKSGCLTDTVQF
                     EQDVLEREKMGGICMGRGLAIPHAKSAGVAHAELAALTLDPPLPCDTPDGEPVRLLFL
                     IAAPADANDLHVQMLAGLATLLLDEEVCRQMLDAPTPTAFCSLIARHEEPAPPPAEES
                     PAAETPPPPPPAQYRLLAVTACPMGVAHTYLAAEALRKAAQERGVTIKVETNGAVGVN
                     DELESDEIAAADCIIIAADKGVSMARCVGKRLVTVSAGDAVRNADAVLNKALNSKVPV
                     YRGGNAFRTSDWRELGRETYRHLMNGISYMIPFVVVGGVMIALSLLLAQMGLAQKYCD
                     LLNNIGKEAFVLIYPVLAASIAVSIGDAPAFMPGLMGGYMAQLGTTAGNKLSWVSSGF
                     WGALIAGFAAGLLVRFLNGLGKKIPENLQQVRSSFL"
BASE COUNT          303 a          490 c          511 g          298 t
ORIGIN      
        1 acgatgagga attcctggac aagcatccca acagccacat tggcgctgag gttgaggttt
       61 ccgagctgat gggcgctgag atctacctgt acctgaccta ccagggccag aacctgatgg
      121 ctcgtgtggc tcccacctcc aagtctcgcc gcggcgacaa gatcgtggtt gccatggata
      181 ccaacaagat tcacctgttc gatcccgaga ccgagctgac cctgctcaac taatccacgt
      241 aaactttata aaagcggctc cgttctggcg gggccgcttt tttcggattg tacaaatttt
      301 ccaaaggggg acatcccaat gcacatcaca gatcacctgc agccctgctg cgtggcgctg
      361 cgccaacagg cggattctct gcctgcggcg atccaccaac tggtggaatt ggtgcagaaa
      421 agcggctgcc tgaccgacac cgtacaattt gaacaggatg tcctggaacg tgaaaaaatg
      481 ggcggcatct gcatgggccg cgggctggcc atcccccacg ccaaaagcgc aggggtggcc
      541 catgccgagc tggccgcctt aacgctggac ccgccgctgc cctgcgatac gccggacggc
      601 gagccggtgc ggctgctgtt tttgatcgct gcaccggcag atgccaacga cctgcatgtg
      661 cagatgctgg cgggcctggc cactctgctg ctggatgagg aagtctgccg gcagatgctg
      721 gatgccccca cgccaacggc tttttgcagc ctgatcgccc ggcacgagga acctgctccg
      781 ccgcctgcag aggagagccc tgccgccgag acgccgccgc ccccgccccc ggcccagtac
      841 cggctgctgg cggttacggc ctgccccatg ggcgtggccc acacctacct ggcggccgag
      901 gctctgcgca aagcggcgca ggagcggggc gtgaccatca aagtggagac caacggcgcg
      961 gtgggcgtga acgacgagct ggaaagcgac gagatcgccg ccgcagactg catcatcatt
     1021 gccgccgaca agggcgtgtc gatggcgcgg tgcgtaggca agcgtctggt gacagtctcg
     1081 gccggtgacg cggtgcgcaa tgctgacgcc gtgctgaata aggcgctgaa cagcaaggtg
     1141 cccgtctacc gcggcggcaa cgccttccgc accagcgatt ggcgggagct gggccgcgaa
     1201 acgtaccgcc acctgatgaa cggcatctcc tacatgattc cctttgtggt ggtgggcggc
     1261 gtcatgatcg ccctgtcgct gcttttggcg cagatgggcc tggcgcaaaa atattgcgac
     1321 ctgctgaata atatcggcaa agaggccttt gtgctgattt acccggtgct ggcggcgtcc
     1381 attgcggtca gcatcggcga cgccccggcg ttcatgccgg gcctgatggg cggctacatg
     1441 gctcagctgg gcaccacggc gggcaataag ctcagctggg tgtcctccgg gttctggggt
     1501 gcgctgattg ccggttttgc ggcaggcctg ctggtgcgtt ttttgaacgg gctgggcaaa
     1561 aaaataccgg aaaacctgca gcaggtgcgc agcagttttt ta
//
LOCUS       DBMDSQ010000416         1591 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_16702_length_1591_cov_1.407383, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000416 DBMDSQ010000000
VERSION     DBMDSQ010000416.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 1591)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1591)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1591
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16702_length_1591_cov_1.407383"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1591)
                     /gene="pheT"
                     /locus_tag="ACZPAX_10640"
     CDS             complement(<1..>1591)
                     /gene="pheT"
                     /locus_tag="ACZPAX_10640"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005924040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HIR1114393.1"
                     /translation="YGHDIQISTGAPNVYAGMHTAAALDGSTLPGGIKIKAKPLMGVE
                     SNGMLCSGEELGLNDDLYPGSEVYGLLDLPKDTVPGTPIQEVVGLDDYIFDISITANR
                     ADCQSVLGIAREVAAVLNKPLKMPATDYTTSDFTDPRLSISVEATDLCPRYIGHYVRN
                     ITPGESPRWMKRQLALCGLRSISNVVDITNYVMLEIGQPMHAFDMDTLESCQILVRRA
                     KDGEKITTLDEKEFTLTPNNLVICDGSKPVALAGVMGGLNSEIKDTTTQLLFESAKFA
                     RDNIRKTARGLGQNTDASSHYEKGISEYTTELGMARALHLIQELGCGEVTSTHFDCSA
                     GAPREGKHFTARISGINAILGITVPTDAILDILNRLQFEVKLEADGDTMQVVAPRWRE
                     DIEVGEPDLAEEVIREYGYDHIVPTFLKAATVTSGGLTAEQRAQAKAKRTMCAQGFYE
                     AETLAFYADADLDMLHIAADAPERNVIRILNPISSHLTIMRPLLAPSLLNACLPEMDN
                     LERFFYYIESFFNLINEDNWLT"
BASE COUNT          307 a          428 c          513 g          343 t
ORIGIN      
        1 gttaaccaat tatcttcatt tataagatta aaaaaacttt ctatatagta gaaaaatcgc
       61 tctaagttgt ccatttcggg caggcaggca ttcagcaggg acggtgccag cagcgggcgc
      121 atgatggtca ggtgggagga aatcgggttc agaatgcgga tgacattgcg ctccggggca
      181 tcggcggcaa tgtgcagcat atctaggtca gcgtcagcgt agaaagccag cgtctcagcc
      241 tcgtagaagc cctgggcgca catggtacgc ttggctttgg cctgagctct ctgctcggcg
      301 gtcaggccgc cgctggtgac ggtggcggct ttcaggaagg tgggcacgat atggtcatag
      361 ccgtactcgc ggatgacttc ctcggccagg tcaggctcac cgacctcgat atcctcgcgc
      421 cagcggggcg caacgacctg catcgtgtcg ccgtcggctt cgagcttgac ctcaaactgc
      481 aggcggttca gaatgtcgag aatggcgtct gtgggaacgg tgatacccaa aatcgcgttg
      541 atgccgctga tcctggcggt gaaatgctta ccctcacgcg gcgcaccggc cgagcagtca
      601 aagtgggtgg aagtgacttc gccgcagccc agctcctgga tcaaatgcag ggcacgggcc
      661 atgccaagct cggtggtata ctcggaaatg cccttctcgt agtggctgga agcgtcggtg
      721 ttctggccca ggccacgagc ggtcttacgg atgttgtcgc gggcaaactt ggcgctctcg
      781 aacaacagct gggtggtcgt atctttaatt tcgctgttca ggccgcccat gacaccggcc
      841 agtgcgaccg gcttggagcc gtcgcagatg acgaggttat tgggagtcag ggtgaactct
      901 ttttcgtcca gcgttgtgat tttttctccg tccttggcgc ggcggacaag aatctggcag
      961 ctttccagcg tgtccatgtc aaaagcgtgc atcggctggc cgatttccag catgacgtag
     1021 tttgtgatat ccacgacgtt ggagatgctg cgtaggccgc agagggccag ctggcgtttc
     1081 atccagcgcg gggactcgcc cggcgtgatg ttgcgcacat agtggccgat gtagcggggg
     1141 cacaggtcgg ttgcctccac gctgatggac aggcgcggat cggtaaagtc gctggtcgtg
     1201 tagtcggtgg caggcatttt caggggcttg ttcagcacag cggcgacctc gcgggcaatg
     1261 ccgagcacgc tctggcagtc ggcacggttg gccgtgatgg agatatcaaa gatgtagtcg
     1321 tcaaggccga caacttcctg aatcggcgtg ccagggacgg tgtccttcgg caaatcgagc
     1381 aggccgtaga cctcggaacc cgggtacagg tcgtcgttca gcccaagctc ctcaccgctg
     1441 cacagcatac cattggactc aacgcccatc aggggcttgg ctttgatctt gatgccgccg
     1501 ggcagcgtgg agccgtccag cgcggcggcg gtgtgcatac ccgcgtaaac gttgggcgca
     1561 ccggtggaaa tttgaatatc gtggccgtac t
//
LOCUS       DBMDSQ010000417         1580 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_16871_length_1580_cov_2.068289, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000417 DBMDSQ010000000
VERSION     DBMDSQ010000417.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 1580)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1580)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1580
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16871_length_1580_cov_2.068289"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(28..1383)
                     /locus_tag="ACZPAX_10645"
     CDS             complement(28..1383)
                     /locus_tag="ACZPAX_10645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008819191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1114394.1"
                     /translation="METKQKTTLTQGSVAKGMLLFALPIFVSNLFQQLYNAADSLIVG
                     NFLGGEALAAVGSSGSLIFLLTGFVNGVSLGAGVVVARYFGAKDWDRMRRTIHTTVAL
                     GLAAGVALTVVGVILTPQILRWMGTPADVLVNSISYFRMYFMGSIAVVMYNVGASILQ
                     SVGDSKSPMEYLITASIINIILDLILVGWLRFGVGAAAFATIASQTVSAILAFAKLMR
                     SQEEWAVRWREVRFDGPSLKAVIVQGLPSGVQNSVISIANVIVQSNINSFGAQAMAGC
                     GAYSKVEGFAFLPVTCFSMALATFVSQNVGAGQSGRVKKGMKFGSLCSVLMAEIVGLC
                     IYFGSPWLIGAFSREPEVIAFGVQQAHTAALFYCLLAFSHCSAGILRGLGRPVVPMMV
                     MLMVWCALRITYITITLHFIHQIGVVFWAYPITWSISSVLFAWYIKHCPIPRLGGGAP
                     E"
     gene            complement(1394..>1580)
                     /locus_tag="ACZPAX_10650"
     CDS             complement(1394..>1580)
                     /locus_tag="ACZPAX_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005945773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HIR1114395.1"
                     /translation="QENDRVTVTPARIKKLESRSFKTESGIEIKIPAELCSSDDAVEF
                     IHSATGGLSLLIKDVLV"
BASE COUNT          369 a          463 c          456 g          292 t
ORIGIN      
        1 ttcgcgatgc atggcagttt tttgtgatta ttccggcgct cccccgccca atcgggggat
       61 tgggcagtgc ttgatatacc aggcaaacag caccgacgag atgctccagg tgatgggata
      121 cgcccagaac acgacaccga tctgatggat aaagtgcagc gtgatggtga tataggtgat
      181 gcgcagcgca caccagacca tcagcatgac catcattggc acgaccgggc ggcccagccc
      241 gcgcagaatg cccgccgagc aatggctgaa cgccagcagg cagtagaaca gtgcggccgt
      301 gtgggcctgc tgcacgccga aggcaatgac ctccggctca cggctgaacg cgccgatgag
      361 ccacggcgac ccaaagtaaa tgcacaggcc gacgatctcg gccatcagca ccgagcagag
      421 ggaaccgaat ttcatgccct tttttacgcg gccagactgg cctgcaccaa cattctggct
      481 gacgaaggtc gccagcgcca tcgagaagca ggtcacaggc aggaaggcaa agccctccac
      541 cttgctgtac gcgccgcagc ccgccatggc ctgcgcaccg aacgagttga tgttggactg
      601 cacaataacg ttggcaatcg aaatgaccga gttctgcacg ccggagggca agccctgcac
      661 aatgaccgcc ttgagcgacg gaccgtcaaa tctgacttcc cgccagcgga cggcccattc
      721 ttcttgggat cgcatcagct tggcgaaggc gagaatggcg ctgaccgtct gggaggcaat
      781 ggtggcgaag gctgccgcgc ccacgccgaa gcgcagccag ccgaccaaaa ttaaatccag
      841 aataatatta atgatggacg ctgtgataag atactccatc gggcttttgc tgtcgcccac
      901 agactgtaaa atactcgcac ctacgttgta cataacaacg gcaatcgagc ccataaagta
      961 catacggaag taggaaatcg agttgacgag cacgtcggcg ggggtgccca tccaacgcaa
     1021 gatctgcggg gtcaggatga cgccgacgac ggtcagcgcc acaccggcgg ccaggcccag
     1081 cgccacggtg gtgtggatgg tgcggcgcat cctgtcccaa tctttggcac cgaaataccg
     1141 tgccactacg acgcccgcac ccagcgaaac gccattgacg aagccggtca gcagaaaaat
     1201 caaactgccg gatgagccga cggcggccag tgcctcgccg cccagaaagt tgccgacgat
     1261 cagggagtcg gcggcgttat acagctgctg gaacaggttg ctgacgaaaa tcggcagcgc
     1321 aaacagcagc atccctttgg ccacgctgcc ctgtgtcagt gtggtttttt gttttgtttc
     1381 catgttttgt tttttacacc aatacgtcct tgatcaacaa actcaacccg ccggtggccg
     1441 aatggatgaa ctcaacggca tcgtcggaac tgcacaattc ggcggggatt ttaatttcaa
     1501 tgccggattc ggtcttgaaa ctgcggcttt ccagcttttt gatgcgggcg ggggtcacgg
     1561 tcacgcggtc gttctcctgc
//
LOCUS       DBMDSQ010000418         1576 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_16919_length_1576_cov_1.955254, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000418 DBMDSQ010000000
VERSION     DBMDSQ010000418.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 1576)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1576)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1576
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16919_length_1576_cov_1.955254"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            117..860
                     /locus_tag="ACZPAX_10655"
     CDS             117..860
                     /locus_tag="ACZPAX_10655"
                     /EC_number="6.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA];
                     GO_function: GO:0061503 - tRNA threonylcarbamoyladenosine
                     dehydratase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HIR1114396.1"
                     /translation="MPDQYTRTRAQLGTPALDALRKAHVAVFGIGGVGGQAVEVLARS
                     GVGELSLFDSDIVALSNLNRQLVALHSTLGQYKVDAMAARIADIDPTIVVHANRMFYN
                     AETAADVDLTQFDYVLDCIDTVSAKLLLIRRCKEAGVSILCSMGAANKLDPTAFKVAD
                     IEKTTVDPLAKVIRIECRKRRLGKVKVVFSEEPPLPPLADETSETPVAARRTVPASNA
                     FVPAACGLVCGSEVVKDILRKAGAYRGTK"
     gene            complement(939..>1576)
                     /locus_tag="ACZPAX_10660"
     CDS             complement(939..>1576)
                     /locus_tag="ACZPAX_10660"
                     /inference="COORDINATES: protein motif:HMM:NF024242.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1114397.1"
                     /translation="LLLVCLFDEKLCEPLTHPYQLSTPLFQDHYNVLERLTAIRQELS
                     EQPRFFEVRTIAMIEELMTDIFRSEPLIANDQQPSEVTARYKQLLDRIDHEFYSITFY
                     DAALFMNVSESYFSRYFKRQAGMTFSQYLNVVRIEKAVQIINAEPDLKVTDVMSRCGF
                     NTIRSFNRAFKLITGYTPRTIPKGYVLNTRSFPAVQGTFDPTLAEAELLAE"
BASE COUNT          363 a          426 c          462 g          325 t
ORIGIN      
        1 ggggccgggt atgcccggcc ctcagccata ccgatacaga acgtttgccg tgaagccgcg
       61 ggccggaaat gtccggcccc tactgggcgg cataaaatat aagggaggaa ccattcatgc
      121 ccgaccaata cacccgcacc cgcgcccaac tgggcacacc cgcgctggac gcattgcgca
      181 aggcccatgt ggccgtgttc ggcatcggcg gcgtcggcgg ccaggccgtt gaggtgctgg
      241 cccgcagcgg ggtaggggag ctgagtttgt ttgacagcga catcgtcgcg ctctccaacc
      301 tgaaccgcca gctggtggcg ctgcactcca cgcttggtca atacaaagtg gacgctatgg
      361 ccgcccgcat tgcggatatc gaccccacca tcgtcgtaca cgctaaccgc atgttttata
      421 acgccgaaac agccgccgat gttgacctga cgcagtttga ttacgtgctg gactgcatcg
      481 acacggtcag cgccaagctg ctgctgatcc gccgttgcaa agaggccgga gtgtcgattc
      541 tgtgcagcat gggcgcagcc aacaagctgg acccgacagc gttcaaagtg gcggatatcg
      601 agaaaaccac cgttgacccg ctggcaaagg tcatccgcat cgagtgccgc aagcgccgcc
      661 tgggcaaggt caaggtcgtt ttttcggagg agccgcccct gccgccgctg gcagatgaga
      721 cgagcgagac cccggttgcc gcacggcgca ccgtgcccgc cagcaatgcc tttgtgcccg
      781 ccgcctgcgg actggtctgc ggatcggagg tcgtcaagga cattctgcgc aaggccgggg
      841 cctaccgcgg cacaaaataa taaaagcaag gataatccaa aaggtgccgt gcaaatggta
      901 gattcccatt cgcacggcac cttttaggtg acgttatatt attctgccag taattcagcc
      961 tcggcaaggg tggggtcaaa agtgccttgt actgcgggga aagatcgtgt gttcagtaca
     1021 tatcctttgg gaattgtacg cggtgtgtaa ccggtgatga gcttgaaggc ccggttaaag
     1081 ctgcgaatgg tgttgaagcc gcaacgcgac atgacatcgg ttaccttcaa gtctggctcg
     1141 gcattgatga tctgcacagc tttttcaatc cgcacaacat tcagatattg cgaaaaggtc
     1201 atgcccgcct ggcgtttgaa gtagcgggaa aagtaagatt ccgaaacatt cataaacagg
     1261 gcggcatcgt aaaaggtaat gctgtaaaat tcgtggtcga tgcggtccag aagctgtttg
     1321 taacgggcgg tgacttcgct tggctgttga tcgtttgcga taaggggttc gctgcgaaag
     1381 atgtccgtca tcaattcctc aatcatggca attgtgcgga cctcaaaaaa gcggggctgt
     1441 tccgaaagtt cctgccgaat ggcagtgagc ctttccagaa cattatagtg atcctgaaaa
     1501 agcggcgttg acagctgata aggatgtgtc agcggttcac agagtttctc atcaaataag
     1561 cataccaaca gcagac
//
LOCUS       DBMDSQ010000419         1570 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_17001_length_1570_cov_1.639210, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000419 DBMDSQ010000000
VERSION     DBMDSQ010000419.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 1570)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1570)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1570
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17001_length_1570_cov_1.639210"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1249
                     /locus_tag="ACZPAX_10665"
     CDS             <1..1249
                     /locus_tag="ACZPAX_10665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004067377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1114398.1"
                     /translation="ADTLMVSGVGEAAVSSVSLVDSLNILIIQILSALATGGAVIASQ
                     YLGRKDEENASHSAAQLYSVLGISTITVGVVCMLLSRPILRGVFGSIDDDVMRFAQQY
                     FLISAVSYPFIGLYNGGAALFRAQGNSRISMLASLVMNIINIAGNALLIYGFGMGVIG
                     AALATLIGRVFAAVFILWQNQNMHNPLRVQHFADLRPRREPIMKVLSIGIPSGLENGM
                     FQIGKLCVSSLTSTLGTSAIAANAVANSVSTLANIPGATMSLATIPVIGQCLGAGEKK
                     QAQRYTMTLMGVAITGLALANLALFILMPEVVTWFSLSAEASTMCTHVVHWFNLFSVF
                     FWATSFTLPNALRAGGDAKYTMTVSIISMWLFRVILSYVFVLQFHMGLTGVWFGMFID
                     WICRSLCFGVRFFRGKWMEHKVI"
     gene            complement(1313..>1570)
                     /locus_tag="ACZPAX_10670"
     CDS             complement(1313..>1570)
                     /locus_tag="ACZPAX_10670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HIR1114399.1"
                     /translation="SHLGEVYTGTVAGITPRGVFVELDNTVEGFVPAAQLCKGEPQVI
                     DGVSMLDPLTGKSWMLGSSMKIQVAAADVALGRIDFEYMVE"
BASE COUNT          286 a          481 c          462 g          341 t
ORIGIN      
        1 ggccgataca ctgatggtct ccggcgtggg cgaagccgct gtttccagcg tttcgctggt
       61 ggacagcctg aatattttga ttattcaaat tttgtctgcc ctggccaccg gcggcgcggt
      121 cattgccagc caatacctgg gccgcaagga cgaggaaaat gccagccata gcgccgccca
      181 gctgtacagc gtgctgggca tttccaccat cacggtgggc gttgtctgta tgctgctctc
      241 ccgcccgatt ctgcgcggcg tgtttggcag catcgacgac gacgtcatgc ggtttgccca
      301 gcagtatttc ctcatcagtg cggtgtcgta tcccttcatc gggctgtaca acggcggcgc
      361 ggcgctgttc cgggcgcagg gcaacagccg tatcagtatg ctggcaagcc tggtcatgaa
      421 catcatcaac atcgcaggca acgcactgct gatctacggc ttcggcatgg gggtcatcgg
      481 cgcagcactg gccaccctga tcggccgtgt ctttgccgcc gtgttcatcc tgtggcagaa
      541 ccagaatatg cacaacccgc tgcgggtcca gcactttgcc gacctgcgcc cccggcgcga
      601 gccgatcatg aaggtgctgt ccatcggtat cccctcgggg cttgaaaatg gtatgttcca
      661 gatcggcaag ctctgcgtca gcagcctgac gtccaccctg ggcacctcgg ccattgcggc
      721 caacgcagtg gcgaactccg tttcgacgct ggcgaacatt cccggcgcga cgatgagcct
      781 agcgacgatt cccgtcatcg gccaatgtct gggcgcgggc gagaaaaagc aggcccagcg
      841 ctacaccatg accctgatgg gcgttgccat aaccggcctg gcgctggcca acctggcgct
      901 gttcatcctg atgcccgagg tcgtgacctg gttcagcctc tcggctgagg cctcgacgat
      961 gtgtacccat gtggtccact ggttcaatct gttcagcgtg ttcttctggg cgacgagctt
     1021 taccctgccc aacgccctgc gcgcgggcgg cgatgccaag tacacgatga cggtcagcat
     1081 catcagtatg tggctgttcc gcgtgatttt gagttatgtt ttcgtcttgc agtttcacat
     1141 gggcctgacc ggcgtctggt tcggtatgtt catcgactgg atctgccgca gcctgtgctt
     1201 cggcgtgcgg ttcttccgtg ggaagtggat ggaacataaa gtgatataag ccacaaaaaa
     1261 tctccctgcc cggcgtggac agggagattt tttatttcat aagggggaaa ccttactcca
     1321 ccatatactc aaaatcaatg cggcccagcg ccacatcggc ggcggcgacc tgaatcttca
     1381 tggaggaacc cagcatccag cttttgccgg tcagggggtc cagcatactc acgccgtcga
     1441 tgacctgcgg ctcgcccttg cagagctggg cggcgggcac aaagccctcg accgtattgt
     1501 ccagctcgac aaaaacgccg cgcggggtga tgcccgccac cgtgccggtg tagacctcgc
     1561 ccagatggct
//
LOCUS       DBMDSQ010000420         1570 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_17007_length_1570_cov_1.277740, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000420 DBMDSQ010000000
VERSION     DBMDSQ010000420.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 1570)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1570)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1570
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17007_length_1570_cov_1.277740"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(55..612)
                     /locus_tag="ACZPAX_10675"
     CDS             complement(55..612)
                     /locus_tag="ACZPAX_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HIR1114400.1"
                     /translation="MELSELTAQARKATEELLEAAHLETGDVFVVGCSSSEIRGGRIG
                     HDSSMEVAAAVLAGVLPPLQEQGVYLAAQCCEHLNRSIVIEREAAKKYGYQIVAAVPQ
                     PHAGGSWATNCWRTFKDPVLVEEVKAAAGMDIGGTLIGMHLRRVAVPVRLSMDHIGEA
                     ILLCARTRPPFIGGSRAVYSDTEVR"
     gene            complement(688..>1570)
                     /locus_tag="ACZPAX_10680"
     CDS             complement(688..>1570)
                     /locus_tag="ACZPAX_10680"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HIR1114401.1"
                     /translation="AEKIIADEQEHLKDYLAEHAALVAECENERTIPGRVRPRLINMS
                     NCRNVWVHGLTLKNGASWNQHMIYSDNITTDHCRFVSEGVWNGDGWDPDSSTNCTLFA
                     CEFATGDDAVAIKSGKNPEGNKIGRPSAHIYVFDCRSTAGHGICLGSEMSGGIEDVQI
                     WDCDLTNSWSGIEIKATPKRGGYVRGVSVRDCTASRLLVHAVPYNDDGEAAPRQPVFS
                     HLSFERLTLTGRGLRDGSFENVEPIELAGFDAPGHELRDVVLDGITVENETGTMTLPV
                     QYCCGLTIRDLTCTARK"
BASE COUNT          266 a          510 c          485 g          309 t
ORIGIN      
        1 tctcgcggat cctggcaacg gcctcggcca ttgcctgctt ctgtgcttcg tccatcaacg
       61 cacctccgtg tcgctgtaca ccgcgcggct gccgccaata aacggcgggc gggtgcgggc
      121 gcacagcaaa atcgcttcac cgatgtgatc catcgacaag cgcaccggca cggctacccg
      181 gcgcaggtgc atgccgatca gagtaccgcc gatgtccata ccggcggcgg ccttcacttc
      241 ctccaccagc acagggtcct taaaggtgcg ccagcagttg gtggcccagc tgccgcccgc
      301 gtggggctgc ggcacggcag ccacgatctg atagccgtac tttttggcgg cttcgcgttc
      361 aatgacgatc gagcggttca gatgctcgca gcactgggcg gccaggtaga cgccctgctc
      421 ctgcagcggg ggcagcaccc cggccagcac ggcggcggca acctccatgc tggaatcatg
      481 gccgatgcgc ccgccgcgaa tctcgctgct ggagcagccg acgacgaaaa catcgccggt
      541 ctccagatgg gcggcttcca gtaattcttc ggtggccttg cgggcctggg cagtcagttc
      601 agaaagttcc ataataaacc tccttgatac gttttatttt gtaggcggaa acgagaacac
      661 gaaaacactt ccaccgtttt gctaaggtta tttgcgggct gtgcaggtca aatcccggat
      721 ggtcagcccg cagcagtact gcaccggcag ggtcatggtg ccggtctcgt tctccacggt
      781 gatgccgtcc agcaccacgt cccgcagctc gtggccgggg gcgtcaaagc ctgccagctc
      841 aatcggctcc acgttctcaa agctgccgtc ccgcaggccg cggccggtca gggtcaaccg
      901 ctcaaagctc aaatggctga acaccggctg gcggggggcc gcttcgccgt cgtcgttgta
      961 gggcacggcg tgtaccagca ggcgggaggc ggtgcagtcc cgcaccgaca cgccgcgcac
     1021 atagccgccg cgcttgggcg tggccttgat ctcaatgccc gaccagctgt tggtcaggtc
     1081 gcaatcccaa atttgcacat cctcgatgcc gccgctcatc tcgctgccca ggcagatgcc
     1141 gtgcccagcg gtgctgcggc aatcgaacac ataaatatga gcgctggggc ggccgatttt
     1201 gttgccctcg ggatttttgc cggacttgat ggccacagcg tcgtcgccgg tggcaaactc
     1261 gcaggcaaac agcgtgcagt tggtgctgga gtccgggtcc cagccgtcgc cgttccacac
     1321 gccctcggat acaaagcggc agtggtcggt agtgatgttg tcgctgtaga tcatgtgctg
     1381 gttccagctg gcgccgtttt tcagcgtcag gccgtgtacc cacacattgc ggcagttgct
     1441 catgttgatc agccgcggcc gcacccggcc ggggatggtt cgttcgttct cgcactcggc
     1501 caccagggcg gcgtgctcgg ccaggtagtc cttcaggtgc tcctgctcgt cggcgatgat
     1561 cttctcagcc
//
LOCUS       DBMDSQ010000421         1560 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_17152_length_1560_cov_1.781357, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000421 DBMDSQ010000000
VERSION     DBMDSQ010000421.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 1560)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1560)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1560
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17152_length_1560_cov_1.781357"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1221)
                     /locus_tag="ACZPAX_10685"
     CDS             complement(<1..1221)
                     /locus_tag="ACZPAX_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR1114402.1"
                     /translation="MSLEDQYDAWVRGLMGGGKYAADEQQAEAASDAVQQMQAQLEAL
                     SAAQKRQSAASSALGAVQKAGAATAENVRKMSSELTKSLKQDGLLGGTVAAPSPAPAA
                     HGKADFTGVTDKIKAQVLGQDAFVSALVKAFRRPFVLGTAEDTTRARSVMLLCGPNGT
                     GRHYALKCVVEELAARGILRSASIETLDLALYPGPAQEKLFLQDLYAALQSDDEVLAF
                     EHYEGCAANYLNMLSTLAIDGTLALSSRYVLQRGILVDVGTALAPGAIGELQAGGKYF
                     VFYSNKGEAALADHFGAKFVDAVAGDICRTEAFTPDALAAVAARELNFLVKRTRKQCG
                     LALSMGADVRDLLAAQYGKTSGMQAMRDYCETVYRAIAEYVLDADATPAEGTPAVLTA
                     VDGKLCMAVNGGKAI"
BASE COUNT          277 a          505 c          489 g          289 t
ORIGIN      
        1 aatggccttg ccgccgttca cggccatgca cagctttccg tccaccgccg tcagcacggc
       61 aggcgtgccc tcggcgggtg tcgcgtcagc atccagcaca tactcggcaa tggcgcggta
      121 gaccgtctca cagtaatcgc gcatggcctg cataccgcta gtcttgccgt actgggctgc
      181 cagtaggtcc cgtacatccg cgcccatgga cagtgccagc ccgcactgct tgcgggtgcg
      241 tttgacgagg aaattcaact cccgcgcggc cacagccgcc agcgcatcgg gggtgaatgc
      301 ctcggtgcgg cagatatcgc ccgcgacggc atccacgaac tttgcgccga agtggtccgc
      361 cagcgcagcc tcgcccttgt tggagtagaa cacaaagtat ttgccgcccg cctgcaattc
      421 gccgatggcc ccgggggcca gcgctgtgcc gacatcgaca aggatgccgc gctgcaagac
      481 gtaccggctg gacagcgcca gcgtaccatc gatggccagc gtggacagca tatttaaata
      541 gttggcggcg cagccctcgt aatgctcaaa ggccagcacc tcgtcgtcgg actgcagcgc
      601 cgcgtaaaga tcctgcaaaa acagcttttc ctgcgcgggg ccggggtaga gcgccaagtc
      661 cagcgtctcg atcgatgcgc tgcgcaggat gccccgggcg gccaattctt ccaccacgca
      721 tttcagcgcg tagtggcggc cggtgccgtt gggaccgcac agaagcatca cgctgcgggc
      781 gcgggtggta tcctccgccg tgcccagcac aaaggggcgg cggaacgcct tgaccagcgc
      841 cgagacgaag gcatcctgcc ccagcacctg ggctttgatt ttatccgtca caccggtaaa
      901 atctgccttg ccgtgggcag ccggggcagg ggagggcgcg gccaccgtgc cgcccagcag
      961 gccatcctgt ttcagcgact ttgtcagttc actgctcatt ttgcgcacat tctcggcggt
     1021 ggcggcaccg gctttctgca ccgcgcccag cgcactgctg gcggcggatt ggcgtttctg
     1081 ggccgcgctc agggcttcca gctgggcctg catctgctgc acggcatcgc tggcagcttc
     1141 ggcctgctgc tcatcggcgg cgtatttgcc gccgcccatc aggccgcgga cccaggcatc
     1201 gtactgatct tccaggctca ttttccatcc tcgatttccg ggtcgatgct gacatcggcc
     1261 cctgcatttt tcttagggca ataccgcata attctaagtg ccgatacggc gagcgaggtg
     1321 cggcagctgc taagccaaaa gcgcagataa tactggatgc tgcaaaggtg agcgccgcca
     1381 gtggcggata cagcgaaccg aagcaggggc agcggtcgca gagtgcgagg ggcttttgcc
     1441 cccgatgcac gatgcgggta ccgcaacccg acattatcga gcattttggc aacgcagatg
     1501 ccgtgccgca gccgccggag cggtgcttaa gccgtaaggt gggaattgtg cggtgttgcc
//
LOCUS       DBMDSQ010000422         1557 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_17217_length_1557_cov_0.768544, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000422 DBMDSQ010000000
VERSION     DBMDSQ010000422.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 1557)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1557)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1557
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17217_length_1557_cov_0.768544"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..247)
                     /locus_tag="ACZPAX_10690"
     CDS             complement(<1..247)
                     /locus_tag="ACZPAX_10690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR1114403.1"
                     /translation="MLANAPAHAYRGQCTYCGHCKPCVANLDIAMINKFYDLATMQPE
                     VPAAVQSHYELLEKTASACIDCHACESRCPFGVKIAER"
     gene            complement(345..1256)
                     /locus_tag="ACZPAX_10695"
     CDS             complement(345..1256)
                     /locus_tag="ACZPAX_10695"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HIR1114404.1"
                     /translation="MKIAVIGPGAMGLLFGSYLSQHHDVTLVGTNPAKMQSITENGVT
                     IRETDGNERIYRPHATADTTGLSPVDLVIVQVKASASEAALTKNKALIGPDTILMTLQ
                     NGMGHETLLSRFAAREQIAIGTTQQGSYKLGETAVCHSGLGKTFLGTVTGDSSRFAPL
                     AEMFAGCGFPCEVCSQVQGMIWDKLMINASSSVLSGILQVAQGYVEQDAHAWTLAEKL
                     IRELCAAATADGYPFDAEEQIARIRLHLQKAPDGFTSIYADLKAGRRTEVDVINGAVV
                     EAGHRHSIPVPTHEFVVELVHAMEGRA"
BASE COUNT          277 a          427 c          475 g          378 t
ORIGIN      
        1 tcctctcggc aatttttacg ccaaaggggc agcggctctc gcaggcatgg cagtcgatgc
       61 aggccgaggc ggtcttttcc agcagttcgt agtgactctg cacagcagcg ggcacttcgg
      121 gctgcatggt ggccaagtcg tagaacttgt tgatcatcgc aatgtccagg ttggccacgc
      181 agggcttgca gtggccgcaa taggtacact ggccgcggta ggcgtgagcc ggggcgttgg
      241 ccagcaccga agcgtagtcc ttatctttgt cttgcccaat ctgcgggttt tcataaggtt
      301 tgcctcctgc tcatttgcgt tgctccgttt atttgctctc gattttatgc tctgccttcc
      361 atggcgtgga ccagttctac cacaaactca tgggtgggca cggggatact gtggcggtgc
      421 ccggcctcca cgaccgcgcc gttgatgacg tccacctcgg tgcggcggcc cgccttcagg
      481 tcggcgtaga tgctggtgaa gccgtcaggc gccttttgca ggtgcagccg gatgcgggcg
      541 atctgctcct cggcgtcgaa agggtaaccg tcggcggtgg cggcggcgca cagctcacgg
      601 atgagcttct cggccagcgt ccaggcgtgg gcgtcctgct cgacgtagcc ctgggccacc
      661 tgcaaaatgc cggacagcac gctggaggat gcgttgatca tcagcttatc ccagatcatg
      721 ccctgcacct ggctgcagac ttcgcagggg aacccgcagc ccgcgaacat ctcggccagc
      781 ggggcaaagc ggctgctgtc gccggtgacg gtgcccaaaa acgttttgcc caggccgctg
      841 tgacagacgg cagtctcgcc caacttataa ctgccctgct gggtggtgcc gatggcgatc
      901 tgctcacggg cggcgaaacg gctcagcaag gtttcgtggc ccataccgtt ttgcagggtc
      961 atcaagatgg tgtcgggacc gatgagggcc ttgtttttgg tcagcgcggc ctcggaggcg
     1021 ctggccttga cctgcacgat gaccaggtcg acaggggaga ggccggtggt gtcggcagta
     1081 gcatgggggc ggtagatgcg ctcgttgccg tccgtctcgc ggatagtaac gccattttcg
     1141 gtaatggact gcattttggc ggggttggtg cccaccaggg taacatcatg gtgttgggac
     1201 aggtagctgc cgaacagcag ccccatcgcg ccgggaccaa tgactgcgat tttcatttac
     1261 gttgctcctt tgttgtgatt tttctattgt ttctattata caaaggtacg gcacagtttg
     1321 gcaagaattg ttaaaatttg cgttatttac ttgactttat caagtaacgg gtatatactt
     1381 gataaagtca agtacgtagg ttgttcccat gtaccacaaa actttatcct attactccac
     1441 cattttttac cgcagctttg ccgcgtttac cggcgagcag ctgcaagccg tagggctcaa
     1501 ctttgggctg ctgttcttcg ttatttacgt aggcaagcac ccgggctgtt caccgtc
//
LOCUS       DBMDSQ010000423         1554 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_17261_length_1554_cov_1.404680, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000423 DBMDSQ010000000
VERSION     DBMDSQ010000423.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 1554)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1554)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1554
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17261_length_1554_cov_1.404680"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..387
                     /locus_tag="ACZPAX_10700"
     CDS             <1..387
                     /locus_tag="ACZPAX_10700"
                     /inference="COORDINATES: protein motif:HMM:NF017452.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HIR1114405.1"
                     /translation="RGVSEGLENRLAAAVREANSMDDLYTRLKTKRYPHARLRRLVLD
                     AALQLPAELPMPPYLHVLGARKTALPRLKQATLPAGTALADLVRSSAEAEKIGRLHSK
                     ATDFSSLCREKIQPMGLAFTAKAVVI"
     gene            472..>1554
                     /locus_tag="ACZPAX_10705"
     CDS             472..>1554
                     /locus_tag="ACZPAX_10705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR1114406.1"
                     /translation="MALHVMDEANHCLGCKVPRCQQGCPIHTNIPEVIRLLKANKLDE
                     AGRMLFENNPLTTVCSLVCNHENQCEGHCVRGIKGTPVHFSVIENYISSTYASQMTQG
                     PAPSNGKKAAIIGSGPAGLTIAVILARYGYDVTIFESHDKIGGVLRYGIPEFRLPKSV
                     LDDFEYRHLRLKGIKVRPNTDVGKALKIEDLFRDGYKAIFIGTGVWRPNTLHIKGESL
                     GNVHYAINYLQNPDVYHLGERAIVIGAGNAAMDVARTAIRHGTRHIECFSLSRHITAS
                     EYEASYAKLEGVNFIFNKKPLEITDKGVVFIDLSEAEDGTLTEIPGTEKLYPADSDIV
                     SISQGPCTTITDSTKDLKTNTRGLFET"
BASE COUNT          375 a          470 c          435 g          274 t
ORIGIN      
        1 cgtggagtca gtgaggggct ggaaaaccgc ctggctgccg ccgtgcggga ggcaaacagc
       61 atggacgacc tgtatacgag actaaaaacc aagcgctacc cccacgcgcg gctgcggcgg
      121 ctggtgctgg acgcggcgct gcagctcccg gcagaattgc caatgccgcc ctacctgcat
      181 gtgctgggtg cacgaaaaac agcgctgccg cgtttaaaac aggcaactct cccggcaggc
      241 acagctctgg ccgacctggt gcgcagcagc gccgaagctg aaaaaatcgg cagactgcat
      301 agcaaggcaa cggatttttc gtcattatgc agagaaaaaa ttcagcctat ggggcttgcg
      361 tttactgcta aggcggtggt aatataaata tcatatggag aaaaaatgcg cataatacaa
      421 gattgttgca tttttaacgc gcaaaatcgg tacgaaatga aggagtaaaa catggcgctg
      481 cacgtaatgg acgaagcgaa ccactgcctt ggctgcaagg tgccccgctg ccagcagggg
      541 tgtcccatcc acaccaatat cccggaagtc atccgccttt taaaagccaa caagctggac
      601 gaagcgggcc gtatgctgtt tgaaaacaac cccctgacca ccgtctgctc gctggtctgc
      661 aaccacgaga accagtgcga ggggcactgc gtgcgcggca tcaagggtac gcccgtgcat
      721 ttctcggtca tcgagaacta catctcgtcc acctacgcca gccagatgac ccagggccct
      781 gcgccctcca acggcaaaaa ggccgccatc atcggttccg gcccggcggg cctgaccatt
      841 gcggtcatcc tggcacgcta tggctacgat gttacgatct ttgagagcca cgataaaatt
      901 ggcggcgtgc tgcgctacgg catcccggag tttcgtctgc cgaaatcggt gctggacgac
      961 ttcgagtacc gccacctgcg cttaaagggc atcaaggtcc gccccaacac cgacgtgggc
     1021 aaggcgctga aaatcgagga cctgttccgc gacggctaca aggccatctt tatcggcacc
     1081 ggtgtatggc ggcccaacac gctgcacatc aagggcgaga gcctgggcaa cgtacattac
     1141 gccatcaact acctgcagaa tcccgacgtc taccacctgg gtgaacgggc cattgttatc
     1201 ggcgcgggca acgcggccat ggacgtggcg cgcaccgcca tccgccacgg cacgcggcat
     1261 atcgagtgct tctcgctgtc ccggcacatc acggccagcg aatacgaggc cagctatgcc
     1321 aagctcgaag gcgtcaactt tatctttaac aaaaagccgc tggaaatcac cgataagggc
     1381 gttgtcttta tcgatctgag cgaggccgag gacggcacgc tgaccgagat ccccggcacc
     1441 gagaagctct acccggcgga cagcgatatt gtctccatca gccagggccc ctgtacgacg
     1501 atcaccgaca gcaccaagga tctgaaaacc aacactcgcg gcctgtttga aacg
//
LOCUS       DBMDSQ010000424         1553 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_17274_length_1553_cov_2.080579, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000424 DBMDSQ010000000
VERSION     DBMDSQ010000424.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 1553)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1553)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1553
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17274_length_1553_cov_2.080579"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1046)
                     /locus_tag="ACZPAX_10710"
     CDS             complement(<1..1046)
                     /locus_tag="ACZPAX_10710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015536893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase family
                     protein"
                     /protein_id="HIR1114407.1"
                     /translation="MSERKLHFETLQLHVGQEQADPVTDSRAVPIYQTTSYVFHSAQH
                     AADRFGLRDAGNIYGRLTNTTEDVFEKRIAALEGGVAALAVASGAAAINYTIQALAQN
                     GGHIVAQKTIYGGTSNLLEHTLPAFGVETTFVNAHDLAEVEGAVKENTRAIYLETLGN
                     PNSDIPDIDAIAEIAHKHNIPLVIDNTFGTPYLIRPIEHGADIVVHSATKFIGGHGTT
                     LGGVIVDSGKFDWKASGKFPAFADPNPSYHGVSFVDAAGPAAFVTYIRAIILRDTGAC
                     ISPFNAFLLLQGTETLSLRLDRHVENTKKVVEYLTHHPKVEKVNHPSLPDHPDHALYE
                     KYFPNGGASIFTFN"
BASE COUNT          321 a          391 c          516 g          325 t
ORIGIN      
        1 atgttgaagg tgaagatgga cgcaccgcca ttggggaagt acttctcata cagggcgtgg
       61 tcggggtggt caggcaggct ggggtggttg accttctcca ccttggggtg atgggtcagg
      121 tactccacga ccttcttggt gttctcgacg tggcggtcca ggcgcaggga gagcgtctcg
      181 gtgccctgca gcagcaggaa cgcgttgaac gggctgatgc aggcgccggt gtcacgcagg
      241 atgatagcgc ggatataggt cacgaacgcg gcgggaccgg cagcatccac gaagctgacg
      301 ccgtggtagc tggggttggg gtcagcaaac gcggggaact tgccgctggc cttccagtcg
      361 aacttgccgg aatcgacgat gacaccaccc agcgtggtgc cgtggccgcc gatgaacttg
      421 gtggcagagt ggacaacgat gtccgcgccg tgctcgatcg gacggatcag gtacggagtg
      481 ccgaaggtgt tgtcgatgac cagcgggatg ttgtgcttgt gggcaatctc ggcgatggcg
      541 tcgatgtcgg gaatgtcaga gttggggtta cccagcgtct ccagatagat ggcgcgggtg
      601 ttctccttga cagcgccttc gacctcggcc aggtcatggg cgttgacgaa ggtcgtctca
      661 acgccgaagg caggcagggt gtgctccaac agattggagg tgccgccgta gatggtcttt
      721 tgggcgacga tatggccgcc gttctgggcc agcgcctgga tggtgtagtt gatggcagct
      781 gcaccggagg caacggccag ggcagcaacg ccgccctcaa gagcggcaat gcgcttttca
      841 aagacatcct cggtggtgtt ggtcaggcgg ccgtagatgt tgccggcatc gcgcaggccg
      901 aaacggtcag cggcgtgctg ggcgctgtgg aaaacgtagg aagtggtctg gtagataggc
      961 acggcgcggg aatcggtgac agggtcagcc tgctcctggc cgacgtgcag ctgaagggtc
     1021 tcaaaatgca gtttacgctc gctcatgata aaatcctcat ttctgttttt attctttaag
     1081 ttgttgacgc aaaagaaaaa ccgggggtcg tacagttaca actcccgggc agaaaataca
     1141 gtgcggcgcg aaattttaca ggcaatgcaa agcaagccca cgcgcccgga cgttttccac
     1201 ccgggagtta ggcattcggc cacagcggaa attctgacgc agcagactgc cgaatacatg
     1261 ggccatatgc tttgcacctc ctttgaattt actaaataac ctataatttc cgtaggtttg
     1321 tctgtattat aatacggata aaccgatatg tcaataggga aaagggaaat ttataaattc
     1381 ctaagccttc cccctttggg ggaaggtggc cccgcagggc cggatgaggg gcgcgtgtgc
     1441 ggcgtcaacc cataaacggg ttatgcggcg aggaggcccc tcatcagtca gcggtcgggg
     1501 ccgctgactg cttcccccct gggggaagcc tcgctcttta taaaatcgtg ccg
//
LOCUS       DBMDSQ010000425         1550 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_17316_length_1550_cov_1.525190, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000425 DBMDSQ010000000
VERSION     DBMDSQ010000425.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 1550)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1550)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1550
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17316_length_1550_cov_1.525190"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            8..1201
                     /gene="coaBC"
                     /locus_tag="ACZPAX_10715"
     CDS             8..1201
                     /gene="coaBC"
                     /locus_tag="ACZPAX_10715"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015565515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HIR1114408.1"
                     /translation="MLLTGKTIVLGITGGIAAYKMPNVAHALVKLGADVHVLMTKNAT
                     EFISPLVFETLTHRRCQVDTFDRNFQYDVAHISLANAADLMLIAPATANVIAKMAHGQ
                     ADDMLTTVTLAATCPKLIAPAMNTHMLENQITQDNLKTLEHYGFTVIPSGSGMLACGD
                     VGSGRLPDEGVLVDYVLRELACEKDLKGKKVVVSAGATQEPMDPVRYLTNHSTGKMGY
                     AVARACMLRGADVTLLASTGCTLPPVPFVNIVPFTTAADLFEAVKANAMDADALVMAA
                     AVADYRPATVAVDKVKKHDGEMNIELERTDDILAWVGVHKPKKLFVCGFSMETRDLIE
                     NSTAKLKKKNMDMIVANNLKVPGAGFGVDTNVVTIITAQGITELPLQSKDAVAGHIAD
                     AIAGK"
     gene            1267..>1550
                     /locus_tag="ACZPAX_10720"
     CDS             1267..>1550
                     /locus_tag="ACZPAX_10720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HIR1114409.1"
                     /translation="MCGIAGQVGRDPRAIQAHYGVYCAMQQTLARRGPDQRGMYISGR
                     AALIHARLAVVDLENGLQPMQLDWKGETYVLVYNGELYNTSELRAALANRG"
BASE COUNT          338 a          471 c          446 g          295 t
ORIGIN      
        1 tgattttatg ttacttaccg gaaaaaccat cgtcctcggc atcaccgggg gcattgccgc
       61 ttacaaaatg cccaatgtgg ctcatgcgct ggtcaagctg ggcgcggatg ttcacgtatt
      121 gatgacgaaa aacgccaccg agtttatctc gccgctggtg tttgagacac tgacccatcg
      181 ccgctgccag gtcgatacct ttgaccgcaa cttccagtac gatgtggccc acatcagcct
      241 tgccaacgcg gcggacctca tgctgattgc cccggccacg gccaacgtca ttgctaagat
      301 ggctcacggc caggcggacg atatgctgac caccgtcacg ctggcagcga cctgccccaa
      361 gctcatcgcc ccggccatga acacccatat gctggaaaac cagatcaccc aggacaacct
      421 gaaaacgctg gagcattacg gcttcacagt cattccctcc ggatccggca tgttggcctg
      481 cggcgatgtc ggctcgggcc gcctgcccga tgagggtgtg ctggtggatt acgttctgcg
      541 agagttggcc tgtgagaagg atctgaaggg caaaaaggtc gttgtctctg ccggtgcgac
      601 ccaggaaccg atggatccgg tgcgctacct gacgaaccat tccaccggta aaatggggta
      661 tgccgtggcc cgcgcctgta tgctgcgcgg cgcagacgtc acgctgctgg cgtccaccgg
      721 ctgcacgctg ccgccggtac cgtttgtaaa catagtgccc ttcaccaccg cggctgacct
      781 gtttgaggct gtcaaggcca acgctatgga cgctgacgcc ctcgtcatgg cggccgctgt
      841 ggcggactac cgcccggcca ccgtggccgt cgataaggtg aaaaaacacg acggcgagat
      901 gaatattgaa ctggagcgca ctgacgatat cctcgcctgg gtcggcgtac acaagccgaa
      961 aaagctgttc gtctgcggct tctcgatgga gacgcgcgat ttgattgaaa actccaccgc
     1021 caagctgaaa aagaagaaca tggacatgat cgtcgccaac aacctgaaag tccccggtgc
     1081 aggtttcggt gttgatacca atgtcgtcac catcatcacc gcccagggca tcaccgaact
     1141 gcccctgcaa agcaaggacg ccgtcgccgg gcatattgcc gacgcgattg caggtaaata
     1201 aaacacaatc atacccctgc cccctcctgc atacacttct acgaatgatg caaagggggc
     1261 atttttatgt gcggaattgc ggggcaggtg gggcgcgatc cccgggccat ccaggcacac
     1321 tatggggtgt actgcgccat gcagcagaca ttggcacggc gtgggccgga ccagcgcggt
     1381 atgtatatca gcggcagggc agcgctcatc cacgcgcggc tggcggtcgt tgacctggaa
     1441 aacggcctgc agcccatgca gctggattgg aagggcgaaa cctacgtcct cgtctacaac
     1501 ggcgaacttt acaacacatc agagctgcgt gccgcgctgg caaaccgcgg
//
LOCUS       DBMDSQ010000426         1545 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_17377_length_1545_cov_2.063712, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000426 DBMDSQ010000000
VERSION     DBMDSQ010000426.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 1545)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1545)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1545
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17377_length_1545_cov_2.063712"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..274)
                     /locus_tag="ACZPAX_10725"
     CDS             complement(<1..274)
                     /locus_tag="ACZPAX_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005932902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HIR1114410.1"
                     /translation="MISAGEFRNGVTFEQDGQVLQVVEFQHVKPGKGAAFVRTKTKNV
                     ITGSVVETSYNPTAKFPQAFIERREVTYSYEDGDLYHFMDVETYDDI"
     gene            complement(392..1255)
                     /locus_tag="ACZPAX_10730"
     CDS             complement(392..1255)
                     /locus_tag="ACZPAX_10730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HIR1114411.1"
                     /translation="MSDTPRFGTAGLADSYTVKKFDPAAIAAYTAGFGLTAFEYQCGR
                     GVRLAHDKAAALGAACAEQNIALSVHAPYYISMSSLEEDKRLNSIDYLLQSCALVKAL
                     GGRRVIFHSGSCGKQSREAALEKALDTMARAVKACDEAGYGDCILCPETMGKINQLGT
                     LDEVLALCEVDKRITPCIDFGHLYARSCGEQFAAATAPADYAALLDTLAARLGDDRAA
                     HFHAHFSRIAYTKGGEKCHLTFADTEFGPPHAPLLALLKERGLAPTIICESAGTQAED
                     AATLAKTYAAL"
     gene            complement(1248..>1545)
                     /locus_tag="ACZPAX_10735"
     CDS             complement(1248..>1545)
                     /locus_tag="ACZPAX_10735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HIR1114412.1"
                     /translation="RVRPFAERIGLDWVPLACKPFTVGLRVARRRLGCQKSEMAMVGD
                     QIFADRMAAGLYGCPCLYLMPRTNHDKNKFVLLKRKFEPYWIDKYYQKGGKLYE"
BASE COUNT          287 a          457 c          478 g          323 t
ORIGIN      
        1 ggatatcgtc gtaggtctca acatccatga aatggtacag atcgccgtcc tcataagagt
       61 aggtgacctc gcggcgctcg atgaaagcct gcgggaactt agcggtcggg ttgtagctgg
      121 tctcaacaac agagccggtg atgacgttct tggtcttggt acggacgaag gcagcgccct
      181 tgcccggctt gacgtgctgg aactcaacga cctgaaggac ctggccgtcc tgctcgaagg
      241 tgacaccgtt acggaattcg cctgcagaaa tcatatagaa attcctccaa tattagatta
      301 tacatacctg tatttatttt acaggaaagt cggcattttt tcaagccctg ttcggacttt
      361 tttgcgtgaa atttgctaaa ttttgtgttt tttatagggc agcataggtt tttgccagcg
      421 tggcggcgtc ctcggcctgc gttccggcag actcgcagat tatggtgggc gctaaaccac
      481 gctccttcag cagcgccagc agcggcgcgt ggggtgggcc aaactcggta tccgcaaagg
      541 tcaagtggca cttttccccg cctttggtgt aggcaatgcg ggaaaaatgc gcgtggaagt
      601 gtgcggcgcg gtcgtcgccc aaacgcgccg ccagcgtgtc cagcagggcg gcgtaatcgg
      661 cgggcgcggt ggcggcggca aactgctcgc cgcagctgcg agcgtacaga tgcccgaaat
      721 cgatgcaggg cgtgatgcgc ttgtcgacct cgcacagggc cagcacctcg tccagcgtgc
      781 ccagctggtt gatcttgccc atcgtctcgg ggcagagaat gcagtcgccg tagccggctt
      841 cgtcgcaggc tttgacagcg cgggccatcg tgtccagcgc cttttccagc gccgcctccc
      901 ggctctgctt gccgcaggag ccggagtgga agatgacacg ccgcccgccc agcgccttga
      961 cgagggcgca gctttgcagc aggtagtcga tgctgttcag ccgcttgtcc tcctccaggc
     1021 tcgacatact gatatagtac ggcgcgtgga ccgagagggc gatattttgc tctgcacagg
     1081 cggcgcccaa ggcggcggcc ttgtcgtggg ccagccgcac gccgcggccg cactggtact
     1141 caaacgccgt caggccaaag cctgccgtgt aggcggcgat ggcggcgggg tcaaactttt
     1201 tgaccgtgta gctgtcggcc agcccggccg tgccgaaacg cggagtgtca ctcatacagc
     1261 ttgccgccct tctgatagta tttgtcgatc cagtacggtt caaacttgcg ctttaacagc
     1321 acaaatttgt ttttgtcgtg gttcgtgcgg ggcatcaggt acaagcaggg gcagccgtac
     1381 agcccggcgg ccatccggtc ggcaaaaatc tggtcgccga ccatggccat ctcgcttttt
     1441 tggcagccca gccgccgccg cgctacccgc aggccgacgg tgaacggctt gcaggccagc
     1501 ggcacccagt ccaggccgat gcgctccgca aagggacgca cccgc
//
LOCUS       DBMDSQ010000427         1538 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_17478_length_1538_cov_1.162143, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000427 DBMDSQ010000000
VERSION     DBMDSQ010000427.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1538)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17478_length_1538_cov_1.162143"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..505
                     /locus_tag="ACZPAX_10740"
     CDS             <1..505
                     /locus_tag="ACZPAX_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006599722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HIR1114413.1"
                     /translation="DTNGCYVVPAFTGLGAPHWDQYARGTIVGLTRGCNKNHIIRATL
                     DSLCYQVNDVLDAMSADSGIRLSTLKVDGGAAANNYLLQTQADISNAPVERPSSVETT
                     ALGAAYLAGLAVGFWSGPEELAKKHAIDKMFVPDISEEERRKRLRGWHKAVRCAYNWA
                     RDDDEDE"
     gene            635..>1538
                     /locus_tag="ACZPAX_10745"
     CDS             635..>1538
                     /locus_tag="ACZPAX_10745"
                     /inference="COORDINATES: protein motif:HMM:NF025104.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HIR1114414.1"
                     /translation="MHYHKSKFLRFANAAFEFLLLILTFIFAGWLRSYTPMGSQFFMW
                     DIWTFLPISALYALVIVGCNAVLGSYRTLHLRNSIKQLFLIALTSLMGLALMATAFYV
                     FRVSQLSRLLLVYYYLLSVIMIYLKRLVFDRLADAYVRKHDIVPRALLIGGGQLAHRF
                     FRDVIEGRGSTAMQYVGYLDAAPREGLPAYLGTVDSLYELLQTHKVDMIVLAQDVQDV
                     ATTQRIMMLADNFHLRVAAVPVYNDYVSSRTEISTLGGMRFVDLRLLNTCEIMGVNIV
                     VTDMEKTVRLLEEKLEDWRGKYICV"
BASE COUNT          300 a          468 c          447 g          323 t
ORIGIN      
        1 ggacaccaac ggctgctacg tggtgcccgc cttcaccggt ctgggcgccc cccactggga
       61 ccagtacgcc cgcggcacca tcgtaggcct gacccgcggc tgcaacaaaa accatatcat
      121 ccgcgccacg ctggacagcc tgtgctatca ggtcaacgac gtgctggacg ccatgagtgc
      181 tgactccggc atccgcctgt ccacgctgaa agtggacggc ggcgcggcgg ccaacaacta
      241 cctgctgcag actcaggccg acatcagcaa cgcgccggtg gagcggcctt cctcggtgga
      301 gaccaccgcc ctgggtgcgg cgtacctggc aggtctggcc gtcggcttct ggtccgggcc
      361 ggaggaactg gcgaaaaaac acgctatcga caaaatgttt gtgcctgaca tctccgagga
      421 agagcgccgc aagcggctgc gcggctggca caaggccgtg cgctgcgcct acaactgggc
      481 gcgggacgac gacgaggacg aataatgcac accccatggc tgcccctttt gccaggagca
      541 gccatttttc tattgaaaaa gcagccttat aatgttatta taaaatgaat ggttgtatcc
      601 ctgtaaccga cagcctggac aacggaggtc ctgcatgcat tatcacaaat ccaaatttct
      661 gcggtttgca aacgccgctt tcgagtttct gctgctgatc cttactttca tctttgccgg
      721 ctggctgcgc agctacacac ccatgggcag ccagttcttt atgtgggaca tctggacgtt
      781 cctgcccatt tcggccctgt atgcgctggt gatcgtaggc tgtaacgccg tgctgggcag
      841 ctaccgcaca ctgcacctgc gcaacagcat caagcagctt tttcttatcg cgctgaccag
      901 cctgatgggc ctggcgctga tggctactgc gttttacgtg ttccgcgtca gccagctttc
      961 ccgtctgctg ctggtatatt attacctgct gtcggtcatt atgatctacc tcaagcggct
     1021 ggtgtttgac aggctggcgg atgcttacgt gcgtaagcac gacatcgtgc cccgggcgct
     1081 gctcatcggc ggcgggcagc tggcccaccg cttcttccgc gatgtgatcg agggccgcgg
     1141 aagcaccgcc atgcagtatg tgggctacct ggacgccgcc ccccgcgagg gtctgcccgc
     1201 ttatctgggt accgtggaca gcctgtatga actgctgcag acccacaagg tcgatatgat
     1261 cgtactggcc caggatgtgc aggatgtagc tactacccag cgcattatga tgctggccga
     1321 caacttccac ctgcgggtag ccgcagtgcc ggtgtacaat gattacgtca gctcccgcac
     1381 cgagatcagc accctgggcg gcatgcggtt cgtggacctg cgcctgctga acacctgcga
     1441 gatcatgggc gtaaatattg ttgtcaccga catggaaaag accgtccgtc tactggagga
     1501 aaagctggag gattggcgcg gcaaatacat ctgcgtgg
//
LOCUS       DBMDSQ010000428         1525 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_17676_length_1525_cov_0.793539, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000428 DBMDSQ010000000
VERSION     DBMDSQ010000428.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1525)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1525
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17676_length_1525_cov_0.793539"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..>404)
                     /locus_tag="ACZPAX_10750"
     CDS             complement(<1..>404)
                     /locus_tag="ACZPAX_10750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1114415.1"
                     /translation="APQQPVAPKADPAATAAAAQAAINSQIEDDAGATAAYDPKSFAA
                     HDEEQDEEDERVRRYAPKGRLFADDDDDDEGTQMYDTDELDDDLDDDDDEDYAPRKKR
                     GRYADDDEVSEDDEMAERIIHWAPPITAIVCAL"
     assembly_gap    405..414
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<415..1050)
                     /locus_tag="ACZPAX_10755"
     CDS             complement(<415..1050)
                     /locus_tag="ACZPAX_10755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1114416.1"
                     /translation="MDQNFSLMAQSRANYYTAGSPVQFVRVELLKGDVTGEVAVCLTF
                     KNVGTEPLTGLVVHFKCKDAAGQVLCEDDFYYEQLNAQPGEVFGSDDAVYVSDTPVSS
                     VEVEQDRAFLNGRGVDLRAYKRVRLQLPRVLPASIARTLQNRTGNAQLTCVPQDNEYG
                     WFCACGAFHPNEENSSVCSECGGDRAQIKATLTNILEDARRAAERQQQEINA"
     gene            complement(1067..>1525)
                     /locus_tag="ACZPAX_10760"
     CDS             complement(1067..>1525)
                     /locus_tag="ACZPAX_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HIR1114417.1"
                     /translation="TGASSDLEQATKTARAMVTRYGFSERLGPVVYGGDPGETFLGRD
                     FGQGKGYSEAIASEIDNEIRDIVDEAYETARRILTEHMAELHKVSAVLMEREKISGEE
                     FKTLMEGGTLPPYDLANGKTAEPAAPAAQAEQPKSDTDAEQVTDQPDVQQ"
BASE COUNT          246 a          464 c          493 g          312 t
ORIGIN      
        1 agcgcacaga caatggcagt gatcggcggg gcccagtgga tgatacgctc ggccatctca
       61 tcgtcttcgc tcacctcgtc atcgtcggca tagcggccgc gctttttgcg aggggcgtaa
      121 tcctcgtcat catcgtcgtc cagatcgtca tctagctcgt cggtgtcgta catctgggtg
      181 ccctcgtcat cgtcgtcatc atcggcaaac agacggccct tgggggcgta gcggcggacg
      241 cgctcgtcct cctcgtcctg ttcctcgtcg tgggcggcaa agctcttggg gtcgtaggcg
      301 gcggtagcac cggcgtcatc ctcgatctgg ctgttgatgg cagcctgggc agcggcagcg
      361 gtggcggcgg gatcagcctt gggggccacg ggctgctgcg gggcnnnnnn nnnnggcatt
      421 aatttcctgc tgctggcgct cggccgcacg gcgggcatcc tccaggatgt tggtcaaggt
      481 cgctttgatc tgggcacggt cgccgccgca ctccgagcag accgaggagt tttcctcatt
      541 gggatggaat gcaccgcagg cgcagaacca gccgtactcg ttatcctgcg gcacgcaggt
      601 cagctgggcg ttgccggtgc ggttctgcag ggtgcgggcg atggaggcgg gcagcacgcg
      661 gggcagctgc aggcgcacac gcttgtaggc gcgcaggtcg acgccgcggc cgttcaggaa
      721 cgcacggtcc tgctcgacct ccacgctgga gaccggcgta tcgctgacat agacagcatc
      781 gtcactgcca aagacctcgc cgggctgggc attgagctgc tcgtaataga aatcgtcctc
      841 gcacaggacc tggcctgcgg cgtccttaca tttgaaatgg acgaccagac cggtcagcgg
      901 ctcagtgccg acgtttttga aggtcaggca aaccgcaact tcgccggtga cgtcaccttt
      961 cagcagttct acgcgaacaa actggaccgg gctaccggcc gtataatagt tggcgcggga
     1021 ctgcgccatc aaggaaaaat tctgatccat ggttcacacc tactttttac tgttgcacgt
     1081 cgggctggtc cgtaacctgc tcggcatccg tatcggattt cggctgttcg gcctgggcgg
     1141 caggtgctgc cggttcggcg gtcttaccgt tggccaggtc atagggcggc agggtgccac
     1201 cctccatcag ggtcttgaac tcttcgccgc tgatcttctc gcgctccatc agcacggccg
     1261 aaaccttgtg cagttcggcc atgtgttcgg tcaggatacg gcgggccgtc tcgtaggcct
     1321 catccacgat gtcgcggatc tcgttgtcga tctcggatgc aatcgcctcg gagtagcctt
     1381 tgccctggcc gaagtcgcgg cccaggaaag tctcgccggg atcgccgccg tagaccacgg
     1441 ggcccagccg ctcagagaag ccataacggg taaccatggc gcgggcagtc ttggtggcct
     1501 gctccaggtc gctggaagca ccggt
//
LOCUS       DBMDSQ010000429         1524 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_17681_length_1524_cov_2.018974, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000429 DBMDSQ010000000
VERSION     DBMDSQ010000429.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 1524)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1524)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1524
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17681_length_1524_cov_2.018974"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            27..1208
                     /locus_tag="ACZPAX_10765"
     CDS             27..1208
                     /locus_tag="ACZPAX_10765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020072711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine/succinylornithine family
                     transaminase"
                     /protein_id="HIR1114418.1"
                     /translation="MNTEKVISRDNDYILHTYGRSQVVLAEGKGMTARDADGKEYLDF
                     TSGIGVNSLGYCHPAWVKAVADQAATLQHTSNLYYTAPDGKLAKKICRRTGLDAVFFG
                     NSGAEANEGAIKCARKYSVDTYGESRSKVITLVNSFHGRTLATLTATGQDVFHHDFGP
                     FPGNFAYVPAGDFAALEKAADASACAVMMELVQGEGGVVALDADYVAKVAAFCKEKDI
                     LLIVDEVQTGVGRTGKFLACEHFDLHPDIVTLAKGLGGGLPIGAVVMNKKVAEHMKPG
                     SHGSTFGGNPVCCAGALAVLDTMDEEFLANVNERAAQLRAGLAKLPHVQQVSGLGLMV
                     GIAFDDGIKAADVRAACEKQGLLVLTAKTRLRLLPPLILTAEDVNAALDTLRTVLEKC
                     V"
     gene            1205..>1524
                     /locus_tag="ACZPAX_10770"
     CDS             1205..>1524
                     /locus_tag="ACZPAX_10770"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015537643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005543 - phospholipid binding [Evidence
                     IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0006593 - L-ornithine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HIR1114419.1"
                     /translation="MKHLLKLGGLTPEEITHILDVADEYKRLHRDGIDPKDLQGKAVA
                     LIFAKNSTRTRASLEVGIYQMGGLGTYLSANDLQTARGEPVQDTARVLGRYYDAIVCR
                     TYKQT"
BASE COUNT          303 a          483 c          475 g          263 t
ORIGIN      
        1 tgcacatgat aaagggggta ccctagatga ataccgaaaa agtgatttcc cgggacaacg
       61 actacatcct ccacacctac ggccgcagcc aggtcgtgct ggcagagggc aagggcatga
      121 ccgccaggga cgccgacggc aaggaatatc tcgattttac gtcgggtatc ggcgtcaact
      181 ccctgggcta ctgccacccc gcctgggtca aggccgtggc agaccaggcc gcgaccttgc
      241 agcacacctc caacctgtat tacactgccc cggacggcaa gctggcaaaa aaaatctgcc
      301 gccgcaccgg gctggatgcg gtattcttcg gcaactcggg tgccgaagcc aacgagggtg
      361 ccatcaagtg cgcccgcaaa tacagcgtag acacgtatgg cgagagccgc agcaaggtca
      421 tcacgcttgt caattccttc catggccgca cgctggcaac cctgaccgcc accggccagg
      481 atgtgttcca ccacgacttt ggacccttcc ccggcaactt tgcctatgtc ccggcgggcg
      541 attttgccgc gctggaaaag gccgccgatg cttctgcctg cgccgtcatg atggagctgg
      601 tacagggcga gggcggcgtt gtcgccctgg atgccgacta tgttgccaag gtcgcggcgt
      661 tctgcaagga aaaagacatt ctgctcatcg tcgatgaggt gcagaccggc gtgggccgca
      721 ccggcaagtt cctggcctgc gagcattttg acctgcaccc cgatatcgtc accctggcca
      781 agggtctggg cggcggcctg cccatcggcg cggtggtcat gaacaaaaag gtcgccgagc
      841 atatgaagcc cggcagccac ggttcgacct tcggcggcaa cccggtctgc tgcgccggtg
      901 cgctggccgt gctggatacg atggacgagg agttcctcgc caatgtcaac gagcgtgcgg
      961 cccagctgcg cgccgggctt gccaaactgc cccatgtgca gcaggtcagc ggcttgggcc
     1021 tgatggtggg catcgccttt gacgacggca tcaaggccgc cgatgtccgc gccgcctgcg
     1081 agaagcaggg cctgctggtg ctgaccgcca agactcgttt gcgcctgctg ccgccgctga
     1141 ttttgacggc ggaggatgtg aacgccgcgc tggataccct gcgcaccgtg ctggagaaat
     1201 gcgtatgaaa catctgctga aattgggcgg cctgacgccc gaggagatca cccacatcct
     1261 ggatgtggcc gacgaatata agcggctgca cagggacggc atcgacccga aggacttgca
     1321 gggcaaggcc gtggcgctga ttttcgccaa aaactccacc cgcacccgcg caagcctgga
     1381 agtgggcatc taccagatgg gcggcctggg cacctacctg agcgccaatg atctgcagac
     1441 tgcccgcggc gagcctgtgc aggacaccgc ccgcgtgctg ggacggtatt atgacgccat
     1501 cgtctgccgc acctacaagc agac
//
LOCUS       DBMDSQ010000430         1523 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_17699_length_1523_cov_1.561181, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000430 DBMDSQ010000000
VERSION     DBMDSQ010000430.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 1523)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1523)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1523
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17699_length_1523_cov_1.561181"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1523)
                     /gene="topA"
                     /locus_tag="ACZPAX_10775"
     CDS             complement(<1..>1523)
                     /gene="topA"
                     /locus_tag="ACZPAX_10775"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015536732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HIR1114420.1"
                     /translation="KYLGDDYEVTASMGHIRDLPASQLGIDVEHGYTPQYISIKGKEK
                     LIKELKSKAKHADGVLLATDPDREGEAISWHLANILGLDPHEPNRVTFDEITKKGVKE
                     GMAHPRAIDEDLFNAQQARRVLDRLVGYKLSPFLWRKVRRGLSAGRVQSVAVRLIDDR
                     EKEIENFKPDEYWNVDATLGAGHKSFVARLATDANGKKLLPKSEAEARAIEKGLEGAS
                     YVVSELKRGKRAKQPTPAFITSTLQQEASRRLGFTATRTMRAAQTLYEGVDIAGHGMM
                     GLITYMRTDSLRISDEAVAAAKEYIAGAYGEAYICPYKRTWKTKSATAAQDAHEAIRP
                     SVPSLTPDEVDKSISGDTAKLYRMIWSRFMASQMADCQQDTVSVTVSAADYHFKASGY
                     TVTFDGFTALYEEATDEKEKKETNLPPLEQGQVLKLRELKSEQKFTQPPARYTEATLI
                     KALEENGIGRPSTYAPIITTIIDRGYVERDQKKLKPTLLGRAVDGLMLEQFPHIVDV"
BASE COUNT          230 a          461 c          480 g          352 t
ORIGIN      
        1 tccacgtcca cgatgtgagg gaactgttcc agcatcagcc cgtccacagc ccgtcccagc
       61 agggtgggct tgagcttttt ctggtcgcgc tccacatagc cgcggtcgat gatggtggtg
      121 atgatgggcg cgtaggtaga gggacggccg atgccgtttt cttccagcgc cttgatgagg
      181 gtagcttcgg tatagcgggc gggcggctgg gtgaacttct gctcgctctt caactcccgc
      241 agcttgagca cctgtccctg ctccagcggg gggaggttcg tttccttctt ttccttttcg
      301 tcggtggctt cctcgtacag ggcggtaaag ccgtcaaagg tcacggtgta gccgctggcc
      361 ttgaagtggt aatccgccgc gctgacggtg accgacacgg tgtcctgctg gcagtctgcc
      421 atctggcttg ccataaagcg gctccagatc atgcggtaca gctttgcggt gtcgccgctg
      481 atgctcttgt ccacctcgtc cggggtcagg gagggcacag agggacggat ggcttcgtgg
      541 gcgtcctgtg ctgccgtggc gctcttggtc ttccatgtgc gcttataagg gcagatatac
      601 gcctcgccgt aggccccggc gatatactcc ttggcggcag ccacggcctc gtccgagatg
      661 cgcagactgt cggtacgcat ataggtgatc agacccatca tgccgtgtcc ggcaatgtcc
      721 acaccttcgt acagggtctg ggcggcgcgc atggtgcggg tggctgtaaa gcccaaccgg
      781 cgggaggcct cctgctgcag ggtactggta ataaaggccg gagtgggctg ctttgcgcgc
      841 ttgccgcgct tgagttcgct caccacatag cttgcgccct ccaagccctt ctcgatggcg
      901 cgggcctcgg cctcgctttt gggcagcagc tttttgccgt tggcgtccgt ggcaaggcgt
      961 gccacaaagc ttttgtgccc ggcacccagg gtggcgtcca cgttccagta ttcatcgggt
     1021 ttaaagtttt cgatttcctt ttcccggtcg tcgatcagcc gcaccgccac gctctgcaca
     1081 cggcctgcgg acaggccgcg gcgcaccttg cgccacagaa acgggctgag cttgtagccc
     1141 accagacggt ccagcacgcg gcgcgcctgc tgggcgttga acagatcctc gtcgatggcg
     1201 cgggggtggg ccatgccctc cttcacaccc tttttggtga tctcgtcaaa ggtgacacgg
     1261 ttgggctcat gggggtccag ccccagaata ttggccagat gccagctgat ggcctcgccc
     1321 tcgcggtccg ggtcggtcgc cagcagcacg ccgtcggcgt gtttggcctt gctcttcaac
     1381 tccttgatga gcttttcctt gcccttgatg ctgatgtatt gcggggtata gccatgctcc
     1441 acatcgatgc ccaactgaga tgcgggcaga tcgcggatat ggcccatgct ggcagtgacc
     1501 tcgtagtcat cgcccagata ttt
//
LOCUS       DBMDSQ010000431         1512 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_17861_length_1512_cov_1.456414, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000431 DBMDSQ010000000
VERSION     DBMDSQ010000431.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 1512)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1512)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1512
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17861_length_1512_cov_1.456414"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..616
                     /locus_tag="ACZPAX_10780"
     CDS             <1..616
                     /locus_tag="ACZPAX_10780"
                     /inference="COORDINATES: protein motif:HMM:TIGR03915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR03915 family putative DNA repair protein"
                     /protein_id="HIR1114421.1"
                     /translation="FGTREILTDRQRAKRVAVGLDRLGPQVKDRIVTGFLSTDPGKDL
                     TLLRFARRCFAQGPQAVQMLGDPDVAAAFAIERAVNNEAGKFIEFIRFEERDGMLGTV
                     IHPKHQVLPLLRGHFCSRLPDEDFLIFDATHGTALVRRGGQVQYLTMEHYTPGTDEDE
                     LNWQQLWKRFFKALTIEERRNEKAQMNHVQKRFWKDMVEMQGSV"
     gene            complement(617..1009)
                     /locus_tag="ACZPAX_10785"
     CDS             complement(617..1009)
                     /locus_tag="ACZPAX_10785"
                     /inference="COORDINATES: protein motif:HMM:NF015898.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HIR1114422.1"
                     /translation="MEQLSRSEEQVMAALWACNRAATRREIASHLPPECHWADSTLLN
                     FLLRLEKKNFVRTEKQGNKNLYTPLVRRLTYCGAVSQAHLQTLYDGDIRAMVRALADT
                     GRMSYADTERLLRFLTEYQAANPEYDYE"
BASE COUNT          320 a          390 c          476 g          326 t
ORIGIN      
        1 gttcggcacg cgggaaatcc tcactgaccg gcagcgcgcg aagcgggtgg cggtggggtt
       61 ggaccggctc gggccacagg tcaaggatcg cattgtaaca gggttcctca gcactgaccc
      121 gggcaaagat ctgactctgc tgcggtttgc gcggcgctgt tttgcccagg ggccgcaggc
      181 cgtgcagatg ctgggcgacc cggatgtggc agcggcgttc gccatcgagc gggccgtcaa
      241 caacgaggcg ggcaagttca tcgagttcat ccggtttgag gagcgggacg ggatgctggg
      301 cacggtcatc cacccgaagc accaagtctt gccgctgctg cgcgggcatt tttgcagccg
      361 cctgccggat gaggattttc tgatttttga cgcgacccac ggcacagcgc tggtgcggcg
      421 cggcgggcag gtgcagtacc tgacgatgga acactacacc cccggcacag acgaggacga
      481 actgaactgg cagcagttgt ggaaacgttt ttttaaggcg ctgaccatcg aggagcgccg
      541 caacgagaaa gcccagatga accacgtgca gaagcggttc tggaaagaca tggtggagat
      601 gcagggcagt gtatgactac tcgtaatcgt actccgggtt ggcagcttgg tactcggtca
      661 ggaagcggag caggcgctcg gtgtcggcgt agctcatgcg gccggtgtca gccagcgccc
      721 gcaccatggc gcgaatgtcc ccgtcgtaga gcgtctgcaa atgcgcctgg ctgaccgcgc
      781 cgcagtaggt caggcggcgc accagcggtg tgtagaggtt cttgttcccc tgcttctcgg
      841 tgcggacgaa gtttttcttt tcaaggcgaa gcagaaaatt gagcagcgtc gagtctgccc
      901 agtggcactc cggcggcagg tgtgacgcga tctcgcgccg ggtggcggcg cgattgcagg
      961 cccaaagtgc ggccatgacc tgttcttcac tgcgggaaag ttgttccatg ataggctcct
     1021 ttttcatagg cgacgatgta ggggccgggc atgcccggcc cggtcgtata agataagtag
     1081 acgattgcgg gaaaacagcg cgggcggagc aaagccccgc tcctacgggc tgcaatttta
     1141 caaatgtgga agtaatatct atatttattt tacatctgtg gtagattgaa atgcaacagg
     1201 aaatattgca caaatctcag ggacttattt tggcataaac agcaaataac ttttacaaat
     1261 gtcgtgcata gtgtccttgc aaagaaaaca atttgtcact ttacagaaac acttgccgga
     1321 ttttgggctt ttttcgtaac tttgcccgct tgctggggcg ggggcaaaat tcctataata
     1381 ttggcatacc gcaacggcgc ggcaagatgc gatccgccgt gtgcgggtcg catttcattt
     1441 actgccaggg aggaaattac gatggctaga gtttacaatt tcctgccagg cccttcgatg
     1501 ctcttctacc ag
//
LOCUS       DBMDSQ010000432         1506 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_17964_length_1506_cov_2.022776, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000432 DBMDSQ010000000
VERSION     DBMDSQ010000432.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 1506)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1506)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1506
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17964_length_1506_cov_2.022776"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            29..790
                     /locus_tag="ACZPAX_10790"
     CDS             29..790
                     /locus_tag="ACZPAX_10790"
                     /inference="COORDINATES: protein motif:HMM:NF017927.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HIR1114423.1"
                     /translation="MWTRTLLKNNAKQALRGRYWRSFWICLALSIAGLGGAGANSGSA
                     AHQAVSTVTDDTTAYDIVNSIPDSMLGAILVGMLIGSIAALCWALFVVYPLNVGRCRY
                     FMESRQSLTPASTVVSTFRRPYGNPIVVQLLTNLKVTLGYILLIVPGVYWSYCYALVP
                     YLLAENPYMTATRAMELSKEMMEGEKWNYFVLQLSFFGWLLLCAFTLGIGGFFLEPYM
                     QATYAEFYAAMRSKALATGMSTTDELGGFVRHDTF"
     gene            959..>1506
                     /locus_tag="ACZPAX_10795"
     CDS             959..>1506
                     /locus_tag="ACZPAX_10795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1114424.1"
                     /translation="MIEVEHLTKRYGGHTAVDDISFTVEDGCIYGLLGPNGAGKSTTM
                     NIITGYISATDGTVKIDGHDIADEPAAAKACIGYLPELPPLYQDMTVQEYLLFVAELK
                     GTRKKADRAAAVEKSAARAGLQGMERRLIRNLSKGYRQRVGIAAALLGTPKVIILDEP
                     TVGLDPAQMIEIRSLIRDLGKT"
BASE COUNT          318 a          459 c          418 g          311 t
ORIGIN      
        1 tagcaatgac tagggaggtg cgacacttat gtggacacgt acacttttaa aaaataacgc
       61 caagcaggca ctcagggggc gctactggcg cagcttttgg atctgcctgg ccctcagcat
      121 tgcaggactg ggcggggcag gggccaacag cggcagtgca gcgcatcagg ctgtcagcac
      181 tgttacggat gacacgacgg cctatgacat tgtcaacagt atccccgaca gtatgctggg
      241 ggcgatcctc gtcggtatgc tcatcggctc tattgccgcg ctttgctggg cactctttgt
      301 cgtctacccg ctcaacgtgg gccgctgccg ctattttatg gagagccgcc aatccctgac
      361 cccggcctcc actgtcgtca gcaccttccg ccgcccctac ggcaacccta tcgtggtgca
      421 gctgctcacc aatttaaaag tcacgctggg ctacatcctg ctcatcgtgc cgggcgtcta
      481 ctggtcgtac tgctatgcgc ttgtgcctta cctgctggct gaaaatccct acatgaccgc
      541 cacccgcgcc atggagctga gcaaggaaat gatggaaggc gaaaagtgga actacttcgt
      601 cctgcagctg tccttctttg gctggctgct gctctgcgcg ttcaccctcg gcattggcgg
      661 tttcttcctg gagccgtata tgcaggccac ctacgccgag ttttatgccg ccatgcgcag
      721 caaggccctt gccacgggta tgtccaccac cgatgaactg ggcggctttg tgcgccatga
      781 taccttttaa atagaactat gctataccgc cgcaggaacg ggccgaaaaa ccgtttctgc
      841 ggcgcttttt gtcggaaagg ggcgcaaggt ttcacacatc ttcaaaccat tatcgcaaaa
      901 ttatcacatt ttaaaagtat aatagaggca accaatgtaa aaagggagtg tgtgcacttt
      961 gatcgaagta gagcatctga caaagcgcta cggcggccac acagcggtgg atgatatcag
     1021 tttcaccgtc gaggatggct gcatctacgg cttgctcggc cccaacggcg cgggcaagtc
     1081 taccaccatg aatatcatca ccggctacat ttccgccacc gacggcacgg tgaaaatcga
     1141 cggccacgac attgccgatg aacctgccgc cgcgaaagcc tgcatcggct acctgccgga
     1201 gctgccgccg ctgtaccagg atatgaccgt gcaggaatat ctgctgtttg tggccgagct
     1261 gaaaggtacc cgcaagaaag ccgaccgcgc cgccgcggtg gaaaagtccg ccgcccgcgc
     1321 gggcttgcag gggatggagc gccgcctcat ccgcaacctg tccaagggct accgccagcg
     1381 cgtgggcatt gccgccgcgc tgctgggcac gccgaaagtc atcatccttg acgagccaac
     1441 cgtcggtctg gatccggcgc agatgatcga gatccgctcc ctcatccgcg acctgggcaa
     1501 gaccca
//
LOCUS       DBMDSQ010000433         1502 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_18031_length_1502_cov_1.558887, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000433 DBMDSQ010000000
VERSION     DBMDSQ010000433.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 1502)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1502)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1502
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18031_length_1502_cov_1.558887"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..945)
                     /gene="asnA"
                     /locus_tag="ACZPAX_10800"
     CDS             complement(<1..945)
                     /gene="asnA"
                     /locus_tag="ACZPAX_10800"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015564658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0070981 - L-asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HIR1114425.1"
                     /translation="MSSIIIPEGYQSLLGLYDTQKAIGLIKTIFQEKICMALHLKRVT
                     APLFVMQGSGLNDDLNGVERPVEFDVPSLNEHAEIVHSLAKWKRYALYKYGFRPGQGI
                     VTDMNAVRRDEELDNLHSIYVDQWDWERVITADQRTLEFLQETVRDIVDAVCATSDEL
                     RWKFPELKNNIHLGRKVTFVTTQELEDIYPDFTPKQRENAYAKEHGTICIMQIGGKLK
                     SGKPHDGRAPDYDDWALNCDILFWHKPLGCALELSSMGIRVDADALRRQLAEAGCPQR
                     AELPFHKMLLDGTLPLTMGGGIGQSRLCMLLLGKAHVGE"
     gene            complement(985..>1502)
                     /locus_tag="ACZPAX_10805"
     CDS             complement(985..>1502)
                     /locus_tag="ACZPAX_10805"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1114426.1"
                     /translation="RDSLRGNLGMVLQETWLKAGTIAENIAYGKPDATREEIIAAAKK
                     ARAHSFICRLPNGYDTQVAEDGGNISQGQRQLLCIARVMLRRPPILILDEATSSIDTR
                     TEVLVQDAFEELMKGRTSFIVAHRLSTIKNADQILVMKAGNIIERGTHDELLAQGGFY
                     KNLYESQFAKA"
BASE COUNT          275 a          448 c          437 g          342 t
ORIGIN      
        1 ttcacctaca tgggccttgc ccaacaacag catgcacagg cggctctggc caatgccgcc
       61 gcccatcgtc aggggcaggg tgccgtccag cagcatcttg tggaagggca gctccgcacg
      121 ctgcgggcag cctgcttcgg ccagctggcg gcgcagggcg tcggcgtcaa cgcggatgcc
      181 catgctggac agttccagcg cacagcccaa aggcttgtgc cagaagagga tatcgcagtt
      241 cagcgcccaa tcgtcatagt cgggggcacg gccgtcgtgg ggcttgccgc tcttgagctt
      301 gccgccgatc tgcataatgc agatggtgcc gtgctccttg gcataggcgt tctcgcgttg
      361 tttcggggtg aagtcggggt atatatcttc caactcctgg gtcgtgacaa atgtcacttt
      421 gcggccaagg tggatgttat ttttcagttc agggaacttc cagcgcagct cgtcagaggt
      481 cgcgcagacc gcatccacaa tatcccgtac agtctcctgt aaaaattcca gcgtgcgctg
      541 gtcggcggtg ataacgcgct cccagtccca ctgatccacg tagatgctgt gcaggttgtc
      601 cagttcctca tcacggcgga cggcgttcat gtcggtcaca atgccctggc cggggcggaa
      661 gccatacttg tacagggcat agcgcttcca ctttgccagg ctgtggacga tctcggcgtg
      721 ctcgttcagg ctgggcacat cgaactcgac cgggcgctcc acaccgttca gatcgtcgtt
      781 caggccgctg ccctgcatga cgaacagggg agccgtgacg cgcttaaggt ggagcgccat
      841 gcagatcttc tcctggaaaa tcgtcttgat caggccgatg gctttctggg tgtcgtacag
      901 gcccaacagg ctttggtagc cttccggaat gataatgctg ctcatattac attctccttt
      961 tcttttccaa atagacccta tttcttacgc tttggcaaac tggctttcgt acaggttctt
     1021 atagaaacct ccctgcgcca gcagttcgtc atgcgtaccg cgctcaatga tgttgccggc
     1081 cttcatcacc aaaatctggt cggcgttctt gatggtggac agacggtggg ccacgataaa
     1141 gctcgtgcgg cccttcatca gttcctcaaa ggcatcctgc accaatacct cggtgcgggt
     1201 atcgatgctg gacgttgcct cgtccaaaat cagaatcggc gggcggcgca gcatgacacg
     1261 cgcaatgcaa agcagctggc gctggccctg gctgatgttg ccgccgtcct cagccacttg
     1321 ggtatcatac ccgttgggca ggcggcagat aaaactgtgg gcgcgggcct ttttcgccgc
     1381 cgcgatgatt tcctcgcggg tagcgtccgg cttgccgtag gcaatgttct cggcaatcgt
     1441 accggctttc agccacgttt cctgcaagac catacccagg ttgccgcgca ggctgtcccg
     1501 gg
//
LOCUS       DBMDSQ010000434         1502 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Gemmiger sp. isolate MAG1304
            NODE_18032_length_1502_cov_1.478944, whole genome shotgun sequence.
ACCESSION   DBMDSQ010000434 DBMDSQ010000000
VERSION     DBMDSQ010000434.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508403
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmiger sp. (human gut metagenome)
  ORGANISM  Gemmiger sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Gemmiger.
REFERENCE   1  (bases 1 to 1502)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1502)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7831x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:36:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,133
            Genes (coding)                    :: 2,120
            CDSs (with protein)               :: 2,120
            Genes (RNA)                       :: 30
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1502
                     /organism="Gemmiger sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18032_length_1502_cov_1.478944"
                     /isolate="MAG1304"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2049027"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..667)
                     /locus_tag="ACZPAX_10810"
     CDS             complement(<1..667)
                     /locus_tag="ACZPAX_10810"
                     /inference="COORDINATES: protein motif:HMM:NF016159.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HIR1114427.1"
                     /translation="MEVTINQVILHLLDPGASEPMLSDRAMEMDADLFEYFTAVLEKA
                     FASDEVKNCRFLPDSAFAAEMAHNNDFVDISRRIAGVIFEQMLQYPTIPAGDLAVVDF
                     NYGAVPHYGVLKLNYRPGYTHNTQVLGNGQVSSMVPQRTLLPGTPKADEAALIDRANG
                     VVRLIEKKYTIDDKKDFYLSTRVFGCTEAMQEKAKLKAVCETAVAAVKEAYPETDDLD
                     DVPP"
     gene            821..1030
                     /locus_tag="ACZPAX_10815"
     CDS             821..1030
                     /locus_tag="ACZPAX_10815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1114428.1"
                     /translation="MKKKHIMSEETLTHTVPCRQKQAKEYLQGRSPGVMRSYAPDAHA
                     DDKDGFVESEDTVHRLAHWREDAIY"
     assembly_gap    1091..1100
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1104..1487)
                     /locus_tag="ACZPAX_10820"
     CDS             complement(1104..1487)
                     /locus_tag="ACZPAX_10820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HIR1114429.1"
                     /translation="MIQVGCKGHAALRVTTENTARAMGSGELPVLATPAVAALVEKTC
                     WQAIAPELELGMTTVGTSLQLAHTAPTPIGMVVQCDCEVLEVTDRRVRFGFRVYDDVT
                     EVASGEHERYLVDAERFLKKAESKI"
BASE COUNT          298 a          467 c          419 g          308 t
ORIGIN      
        1 aggggggcac atcgtccagg tcgtcggtct cggggtaggc ttccttcacg gcggccacgg
       61 cggtctcgca gacggccttc agcttggcct tctcctgcat ggcctcggtg cagccaaaaa
      121 cccgcgtaga caggtaaaaa tccttcttgt cgtcgatggt gtacttcttc tcgatcaacc
      181 gcaccacgcc gttggcgcgg tcgatcagcg cggcttcgtc ggccttgggg gtgccgggca
      241 gcagcgtgcg ctggggcacc atgctgctga cctgcccgtt gcccagcacc tgggtgttgt
      301 gggtgtagcc ggggcggtag ttcagcttca gcacgccgta gtggggcacc gcgccgtagt
      361 taaaatccac cacggccaga tcgcccgccg ggatggtggg atactgcagc atctgctcaa
      421 agatgacgcc cgcaattctg cgcgaaatat cgacaaaatc gttgttgtgg gccatctcgg
      481 cagcaaaggc agagtccggc aggaaccggc agtttttgac ctcgtcgctg gcgaaagcct
      541 tctccagcac ggcggtgaaa tattcaaata ggtcggcatc catctccatg gcccggtcgg
      601 acagcatcgg ctccgacgcg ccggggtcca gcaggtgcag gatgacctga ttgatcgtga
      661 cttccatttg gttcgctcct ttgtttggca taggaaacag tataccatat tttggcgggg
      721 atggccagtg gaattgcggg aacacttact ttatggaaag tatcccccac cgtaggggcc
      781 gatgctcgtc atcggcctgc ccgtgaaagg agcaatcacg atgaaaaaga agcatattat
      841 gtccgaggaa acattaaccc acaccgtccc ctgccgccaa aaacaggcca aagaatacct
      901 gcagggccgt tcccccggcg tgatgcgatc ctacgccccc gatgcccacg ccgatgataa
      961 agacggcttc gttgaatccg aagacaccgt ccaccgcctg gcccactggc gggaagacgc
     1021 gatttattga tccatgttct tatgccgcgg ccgcgctctt gccttaacgg aaacattcca
     1081 ttataaaagg nnnnnnnnnn acgtcaaatt ttactctcgg cttttttcaa aaaccgttcg
     1141 gcatccacaa ggtaccgttc atgttccccg ctggccacct cggtcacatc atcatacacg
     1201 cggaacccaa aacgcacccg gcggtcggtc acttccaaaa cctcacaatc gcactgcacc
     1261 accatcccga tgggcgtcgg cgcggtgtgg gccagctgca aactggtgcc tacggtggtc
     1321 atgcccagct ccagttcggg cgcgatggcc tgccagcagg tcttttccac cagcgccgcc
     1381 acggcggggg tcgccagcac cggcagctcg ccgctgccca tcgcccgggc ggtgttctcg
     1441 gtggtcaccc gcagcgccgc atgccctttg catccaactt gtatcatggt atcctcctat
     1501 aa
//
